LOCUS       DBNICI010000001        72252 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.001, whole genome shotgun sequence.
ACCESSION   DBNICI010000001 DBNICI010000000
VERSION     DBNICI010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 72252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.001"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(132..854)
                     /locus_tag="AC1ZCC_000001"
     CDS             complement(132..854)
                     /locus_tag="AC1ZCC_000001"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIQ1628293.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(844..1581)
                     /locus_tag="AC1ZCC_000002"
     CDS             complement(844..1581)
                     /locus_tag="AC1ZCC_000002"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1628294.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(1593..2585)
                     /locus_tag="AC1ZCC_000003"
     CDS             complement(1593..2585)
                     /locus_tag="AC1ZCC_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIQ1628295.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            complement(2600..3343)
                     /locus_tag="AC1ZCC_000004"
     CDS             complement(2600..3343)
                     /locus_tag="AC1ZCC_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1628296.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(3417..3521)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3584..4906)
                     /locus_tag="AC1ZCC_000005"
     CDS             complement(3584..4906)
                     /locus_tag="AC1ZCC_000005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase family protein"
                     /protein_id="HIQ1628297.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(4893..5858)
                     /locus_tag="AC1ZCC_000006"
     CDS             complement(4893..5858)
                     /locus_tag="AC1ZCC_000006"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1628298.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(5855..7393)
                     /locus_tag="AC1ZCC_000007"
     CDS             complement(5855..7393)
                     /locus_tag="AC1ZCC_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HIQ1628299.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(7809..8096)
                     /locus_tag="AC1ZCC_000008"
     CDS             complement(7809..8096)
                     /locus_tag="AC1ZCC_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628300.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            8230..8898
                     /locus_tag="AC1ZCC_000009"
     CDS             8230..8898
                     /locus_tag="AC1ZCC_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIQ1628301.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            complement(9189..9929)
                     /locus_tag="AC1ZCC_000010"
     CDS             complement(9189..9929)
                     /locus_tag="AC1ZCC_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628302.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(10059..11006)
                     /locus_tag="AC1ZCC_000011"
     CDS             complement(10059..11006)
                     /locus_tag="AC1ZCC_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIQ1628303.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(11135..11899)
                     /locus_tag="AC1ZCC_000012"
     CDS             complement(11135..11899)
                     /locus_tag="AC1ZCC_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIQ1628304.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(11903..12292)
                     /locus_tag="AC1ZCC_000013"
     CDS             complement(11903..12292)
                     /locus_tag="AC1ZCC_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628305.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            12427..12969
                     /locus_tag="AC1ZCC_000014"
     CDS             12427..12969
                     /locus_tag="AC1ZCC_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HIQ1628306.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13117..13749
                     /locus_tag="AC1ZCC_000015"
     CDS             13117..13749
                     /locus_tag="AC1ZCC_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628307.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(13883..14008)
                     /locus_tag="AC1ZCC_000016"
     CDS             complement(13883..14008)
                     /locus_tag="AC1ZCC_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HIQ1628308.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(14142..15035)
                     /locus_tag="AC1ZCC_000017"
     CDS             complement(14142..15035)
                     /locus_tag="AC1ZCC_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HIQ1628309.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(15217..16143)
                     /locus_tag="AC1ZCC_000018"
     CDS             complement(15217..16143)
                     /locus_tag="AC1ZCC_000018"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HIQ1628310.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(16228..16989)
                     /gene="pflA"
                     /locus_tag="AC1ZCC_000019"
     CDS             complement(16228..16989)
                     /gene="pflA"
                     /locus_tag="AC1ZCC_000019"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HIQ1628311.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(17233..19464)
                     /gene="pflB"
                     /locus_tag="AC1ZCC_000020"
     CDS             complement(17233..19464)
                     /gene="pflB"
                     /locus_tag="AC1ZCC_000020"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HIQ1628312.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(19741..22191)
                     /gene="parC"
                     /locus_tag="AC1ZCC_000021"
     CDS             complement(19741..22191)
                     /gene="parC"
                     /locus_tag="AC1ZCC_000021"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HIQ1628313.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(22203..24248)
                     /gene="parE"
                     /locus_tag="AC1ZCC_000022"
     CDS             complement(22203..24248)
                     /gene="parE"
                     /locus_tag="AC1ZCC_000022"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HIQ1628314.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(24414..24848)
                     /locus_tag="AC1ZCC_000023"
     CDS             complement(24414..24848)
                     /locus_tag="AC1ZCC_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIQ1628315.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            24975..25613
                     /gene="plsY"
                     /locus_tag="AC1ZCC_000024"
     CDS             24975..25613
                     /gene="plsY"
                     /locus_tag="AC1ZCC_000024"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HIQ1628316.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(25745..26620)
                     /locus_tag="AC1ZCC_000025"
     CDS             complement(25745..26620)
                     /locus_tag="AC1ZCC_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HIQ1628317.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(26702..27493)
                     /gene="codY"
                     /locus_tag="AC1ZCC_000026"
     CDS             complement(26702..27493)
                     /gene="codY"
                     /locus_tag="AC1ZCC_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HIQ1628318.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(27511..28911)
                     /gene="hslU"
                     /locus_tag="AC1ZCC_000027"
     CDS             complement(27511..28911)
                     /gene="hslU"
                     /locus_tag="AC1ZCC_000027"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HIQ1628319.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(28925..29473)
                     /gene="hslV"
                     /locus_tag="AC1ZCC_000028"
     CDS             complement(28925..29473)
                     /gene="hslV"
                     /locus_tag="AC1ZCC_000028"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HIQ1628320.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(29510..30415)
                     /gene="xerC"
                     /locus_tag="AC1ZCC_000029"
     CDS             complement(29510..30415)
                     /gene="xerC"
                     /locus_tag="AC1ZCC_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HIQ1628321.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(30613..31989)
                     /gene="trmFO"
                     /locus_tag="AC1ZCC_000030"
     CDS             complement(30613..31989)
                     /gene="trmFO"
                     /locus_tag="AC1ZCC_000030"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HIQ1628322.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(31958..34036)
                     /gene="topA"
                     /locus_tag="AC1ZCC_000031"
     CDS             complement(31958..34036)
                     /gene="topA"
                     /locus_tag="AC1ZCC_000031"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIQ1628323.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(34158..35015)
                     /gene="dprA"
                     /locus_tag="AC1ZCC_000032"
     CDS             complement(34158..35015)
                     /gene="dprA"
                     /locus_tag="AC1ZCC_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIQ1628324.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(35071..35838)
                     /locus_tag="AC1ZCC_000033"
     CDS             complement(35071..35838)
                     /locus_tag="AC1ZCC_000033"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIQ1628325.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(35831..36691)
                     /gene="ylqF"
                     /locus_tag="AC1ZCC_000034"
     CDS             complement(35831..36691)
                     /gene="ylqF"
                     /locus_tag="AC1ZCC_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HIQ1628326.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            36951..37349
                     /locus_tag="AC1ZCC_000035"
     CDS             36951..37349
                     /locus_tag="AC1ZCC_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIQ1628327.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            37586..38128
                     /locus_tag="AC1ZCC_000036"
     CDS             37586..38128
                     /locus_tag="AC1ZCC_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HIQ1628328.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(38211..38762)
                     /gene="lepB"
                     /locus_tag="AC1ZCC_000037"
     CDS             complement(38211..38762)
                     /gene="lepB"
                     /locus_tag="AC1ZCC_000037"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIQ1628329.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(38914..39141)
                     /locus_tag="AC1ZCC_000038"
     CDS             complement(38914..39141)
                     /locus_tag="AC1ZCC_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HIQ1628330.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(39138..39656)
                     /gene="msrA"
                     /locus_tag="AC1ZCC_000039"
     CDS             complement(39138..39656)
                     /gene="msrA"
                     /locus_tag="AC1ZCC_000039"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIQ1628331.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(39676..40389)
                     /locus_tag="AC1ZCC_000040"
     CDS             complement(39676..40389)
                     /locus_tag="AC1ZCC_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HIQ1628332.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(40392..41288)
                     /locus_tag="AC1ZCC_000041"
     CDS             complement(40392..41288)
                     /locus_tag="AC1ZCC_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIQ1628333.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(41446..42288)
                     /locus_tag="AC1ZCC_000042"
     CDS             complement(41446..42288)
                     /locus_tag="AC1ZCC_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIQ1628334.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            42420..43073
                     /gene="trhA"
                     /locus_tag="AC1ZCC_000043"
     CDS             42420..43073
                     /gene="trhA"
                     /locus_tag="AC1ZCC_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAQR family membrane homeostasis protein TrhA"
                     /protein_id="HIQ1628335.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(43136..43663)
                     /locus_tag="AC1ZCC_000044"
     CDS             complement(43136..43663)
                     /locus_tag="AC1ZCC_000044"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HIQ1628336.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(43683..44630)
                     /locus_tag="AC1ZCC_000045"
     CDS             complement(43683..44630)
                     /locus_tag="AC1ZCC_000045"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIQ1628337.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(44642..46525)
                     /locus_tag="AC1ZCC_000046"
     CDS             complement(44642..46525)
                     /locus_tag="AC1ZCC_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIQ1628338.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(46688..48766)
                     /locus_tag="AC1ZCC_000047"
     CDS             complement(46688..48766)
                     /locus_tag="AC1ZCC_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIQ1628339.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(48779..49054)
                     /locus_tag="AC1ZCC_000048"
     CDS             complement(48779..49054)
                     /locus_tag="AC1ZCC_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628340.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(49416..49871)
                     /locus_tag="AC1ZCC_000049"
     CDS             complement(49416..49871)
                     /locus_tag="AC1ZCC_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628341.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(50025..51233)
                     /locus_tag="AC1ZCC_000050"
     CDS             complement(50025..51233)
                     /locus_tag="AC1ZCC_000050"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HIQ1628342.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(51345..51677)
                     /locus_tag="AC1ZCC_000051"
     CDS             complement(51345..51677)
                     /locus_tag="AC1ZCC_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HIQ1628343.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            51825..52700
                     /locus_tag="AC1ZCC_000052"
     CDS             51825..52700
                     /locus_tag="AC1ZCC_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIQ1628344.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(52782..53363)
                     /locus_tag="AC1ZCC_000053"
     CDS             complement(52782..53363)
                     /locus_tag="AC1ZCC_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HIQ1628345.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(53369..54628)
                     /locus_tag="AC1ZCC_000054"
     CDS             complement(53369..54628)
                     /locus_tag="AC1ZCC_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIQ1628346.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(54630..55676)
                     /locus_tag="AC1ZCC_000055"
     CDS             complement(54630..55676)
                     /locus_tag="AC1ZCC_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628347.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(55911..56186)
                     /locus_tag="AC1ZCC_000056"
     CDS             complement(55911..56186)
                     /locus_tag="AC1ZCC_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIQ1628348.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(56429..57739)
                     /gene="der"
                     /locus_tag="AC1ZCC_000057"
     CDS             complement(56429..57739)
                     /gene="der"
                     /locus_tag="AC1ZCC_000057"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIQ1628349.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(57921..59150)
                     /gene="rpsA"
                     /locus_tag="AC1ZCC_000058"
     CDS             complement(57921..59150)
                     /gene="rpsA"
                     /locus_tag="AC1ZCC_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIQ1628350.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(59285..59965)
                     /gene="cmk"
                     /locus_tag="AC1ZCC_000059"
     CDS             complement(59285..59965)
                     /gene="cmk"
                     /locus_tag="AC1ZCC_000059"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIQ1628351.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(60014..60649)
                     /locus_tag="AC1ZCC_000060"
     CDS             complement(60014..60649)
                     /locus_tag="AC1ZCC_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HIQ1628352.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(60715..62118)
                     /locus_tag="AC1ZCC_000061"
     CDS             complement(60715..62118)
                     /locus_tag="AC1ZCC_000061"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HIQ1628353.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(62105..63136)
                     /locus_tag="AC1ZCC_000062"
     CDS             complement(62105..63136)
                     /locus_tag="AC1ZCC_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1628354.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            63167..63388
                     /locus_tag="AC1ZCC_000063"
     CDS             63167..63388
                     /locus_tag="AC1ZCC_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HIQ1628355.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(63553..64290)
                     /locus_tag="AC1ZCC_000064"
     CDS             complement(63553..64290)
                     /locus_tag="AC1ZCC_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HIQ1628356.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(64443..65783)
                     /locus_tag="AC1ZCC_000065"
     CDS             complement(64443..65783)
                     /locus_tag="AC1ZCC_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIQ1628357.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(66020..67543)
                     /locus_tag="AC1ZCC_000066"
     CDS             complement(66020..67543)
                     /locus_tag="AC1ZCC_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HIQ1628358.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(67921..68532)
                     /locus_tag="AC1ZCC_000067"
     CDS             complement(67921..68532)
                     /locus_tag="AC1ZCC_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIQ1628359.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(68655..68780)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(68862..69578)
                     /locus_tag="AC1ZCC_000068"
     CDS             complement(68862..69578)
                     /locus_tag="AC1ZCC_000068"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIQ1628360.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(69584..70153)
                     /gene="scpB"
                     /locus_tag="AC1ZCC_000069"
     CDS             complement(69584..70153)
                     /gene="scpB"
                     /locus_tag="AC1ZCC_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIQ1628361.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(70137..70907)
                     /locus_tag="AC1ZCC_000070"
     CDS             complement(70137..70907)
                     /locus_tag="AC1ZCC_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HIQ1628362.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(70904..71275)
                     /locus_tag="AC1ZCC_000071"
     CDS             complement(70904..71275)
                     /locus_tag="AC1ZCC_000071"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1628363.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(71294..72181)
                     /gene="xerD"
                     /locus_tag="AC1ZCC_000072"
     CDS             complement(71294..72181)
                     /gene="xerD"
                     /locus_tag="AC1ZCC_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HIQ1628364.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT        19923 a        15220 c        12320 g        24789 t
ORIGIN      
        1 tcttgggaag cggcgaaagc attctgggaa gcaaacggtt ggtactaaga ttcatttatt
       61 aatcaaacag tttactttta tacaaagaaa gccaaaaaga ggtgcgacac tagttgtgtc
      121 acatctcttt tttatttacc taatctaatg aaacgttcca agttttgata agtatttttt
      181 ttaacttctt caactgatag ctctttgatc tcagcgattg tctttaactg ttcttcaagc
      241 agtgagacat aatctttttc tgaaaaaatc ccccctaaag cccactctgc tgcatgtagt
      301 ccatccgttt caataagtaa gtggtcgatt gggactgctt tagctagttg ccagactgct
      361 ggattctttt tcaaatcaag accaatgctg aaataacagc ctaaatcaat atagtcttgt
      421 tgatagtcca aagaagaata ccaatggatc aaatagtgat tagggtaagc ttggatataa
      481 tttaagatct ctttttcgca tccttttgtg tgcagtacaa caggtttctc atttatggct
      541 gcaaaagcaa gctgtcgctc aaatactttt ttttgtgtag tcatcggtac attcgtccaa
      601 atattatcta accctatttc accgatgatc cttgcttttt tcaaaaaagg ctcaacttgt
      661 tcaaacgtat agctatctgc tttccaagga tggataccgt aacttaaggc ttgagtctta
      721 cttgctgcaa gctgtctgtt ttccttccat tcttctggcg aatcacaatt gatgatcgtc
      781 aaaatcgtgt gttcctgctg aaggagtgct aaactctgac tcccatctaa atgacaatga
      841 gcatcactta acatctaatt cacctgccaa taactgataa atcgtttgct tcaactctga
      901 ttgctgcagt agccattcac gtgaccgtcg tttttcaggc acttgaatct cagcaatcac
      961 atgtgccgga cgggcggaaa gcacaagaat cctgtctgat aaataaattg cttcttcgat
     1021 atcatgcgtc acaagtaaag tagtccgctt tgctttttta ggcagcttca acatccagtc
     1081 ttgcatctgc cctctggtca tagcatctaa cgctccaaat ggttcatcaa gaagcaagta
     1141 agaagaagga ttcatgattg ttcgtaacaa ggccgttcgc tgcttcatcc cgccagataa
     1201 ttgttccggc aaaaaatgtt catagccgtt taacccaaag atatccagat aatcccttac
     1261 ttgttcttca ttgattgtta gatgattgat tttttgatat aacgtaacgt tttgatagac
     1321 gctcagccaa ggaagtaaca tatcttcttg cggcatataa ctaaatgctg tatggagacc
     1381 agttactttt tgatcgtcta ctttgatcgt tccagaagtc ataggctgga ctcctgttaa
     1441 agttttcagc aatgttgact taccggctcc gcttggcgcg acaatcgatg taaaggtatc
     1501 tttagggata gtgaaatgga tgtcttctaa tattttcagg ttttcaatga ctacattcaa
     1561 gtggctgatt tggattgaca tatctatcac tcctatttta taaactgatt agtgaatagt
     1621 tcttgcgaag gtacatcttg cttgatcaat ccgttttcct gcataaaagc tgtataattg
     1681 cgccaaactt tttcttccat caatccccaa ttctcaggat catccgtata attttttgac
     1741 aagaatgctt gtgattcttc taaaaacgtc cgatcatttt cttttgcata tttttgcaac
     1801 agtttagcac tgtcatttgg atccttaata gcttcctgat agcctttttt cgtggcatcc
     1861 ataaaagact gtaccaattc tggatcactt ttgatcagtt gatcattcgt aataattacc
     1921 ggagtatagt aatctaaccg ttcatctaat tctttcaatg ggatatagtt tagttcaata
     1981 cccgcttctt ttgctttgat catatcccaa ccttcaaagt accattggat atctgaggat
     2041 tttcctaagc tttctggccc ttctccattg cttcctacca ttgtcagttt tgaaaagtcc
     2101 ccgccgtctt tttccatcac agcttttaag acagcttctt cacttggact ttgccaacct
     2161 gcatatgttt ttccttcaaa atcttttggt gaatggatgt tactgtctga tagggtggcg
     2221 aatccagaag tattatgttt gatcactgta gcaattgctt tgactggtat attctgtcca
     2281 tctgcgtgag cataagtgac atcttcttga taacttacac caaattgtgc tttgtccgca
     2341 cctactagac caatacttgt cgaattatct cctggttcga tgatttggac atttaaacca
     2401 gcctctttat aatagccttt ttcttttgct aggtagatgc cagtatgatt agtattcggt
     2461 acataatcca aaacaagtgt cacttttttc aaatccttta tttcttgttt gctacttgtt
     2521 tcttttgcag tgccacaagc ggataaaagc aacagtagcg gtaataaaag ccataatttt
     2581 ttcatttgat ccccttctct taaaaaatat aaagcatacg ataataccct tttttcaata
     2641 atgaacaagc gccattcagc aataaactca atagaatgac acaaatgatc gcagcaaaaa
     2701 ctttatcgat ctggtagctg ttttttacgc gtatcatgta ataccctaag cctgcttgag
     2761 cgcttagcca ttctccaacg actgctccgc tgacacaata agtagcagcc acttttaaac
     2821 cggaaaagaa gccggataaa cccgctggta ttttatatat tcgataaatc ttccctgtat
     2881 ctgcccccat cgttttcaaa aagatgattt gctcttttga tactttcctc aatgtatccg
     2941 aaaaagaaac tacgatcgga aaatagctta tcaagacaac aaggatcact tttggaagca
     3001 taccgaaacc aaaccatagg gtaagtaatg gccctaacac catgattggc aacatttgtg
     3061 aaatcactag taacggataa aaagacattt ctaaaaactt ccaataatcc attgccaagg
     3121 cagtaataaa agaaagaacg ctagctatta ccaaaccaag cactgcttca tttaatgtcg
     3181 tgacagaatg ggaaagtaat acttgtgcat ctgccgctaa tgcagaaaat acagcagcag
     3241 gtccgggtaa aataaaggca ggtattttcc aaactatgac aacaatctgc caaataatta
     3301 aaatgaatgc catactgact gctggataaa gatttttctt cattttgtta cctcctttct
     3361 tctaatatga ataacaaaaa acgcttgaaa tgggcacgac atagtaaccc gcttcaagcg
     3421 tctgctatgt ttctcccttc accagtatta actgacaggt tcaatgggtc taagttcaca
     3481 acttactctc atgtcgtcta cgacatcccc agaaacaggt attcattttt taaagtgtag
     3541 cacaagaagg aaaaagcaac aagcgcttta gttatcatgc tgattatttc aataaatagg
     3601 agagatcact tgccaatgtc ggatacccca tgatatagct atccaactct tttttagtca
     3661 atttttgatt gatgacgagt gtcaaatcat cgatcaattc gtctgcttgc tcgctaatca
     3721 ctgcagcacc aatcaaataa ttttgctggt caaaggtcaa ttcagcctta gcaactggtt
     3781 cattgacacg atgataggtg aaccaactgg taagatccat ctctaccact tgatctgacg
     3841 aagaggcgtg actttttgta actcctactt cagcaagttt aggactggcg tagacgatcg
     3901 taggtataat cggatatttg ataggttcgc ttgttgcatc cgttatccgc ttagctacat
     3961 aatttccttc aaaagtggcg actggtgtca attttggggt tttccttgaa acaatatctc
     4021 cacacgcaaa aatttttgga ttgctcgttt gcagataatc attgactgca attccgtgtt
     4081 tatcaaagac gatattcgct tgctcaagtg ctaaagattc tgtattcggc tgtctcccag
     4141 tcgcacaaaa aatcatatct gctactaatt ccgtctcttt tccgactaaa cgatagcgag
     4201 taccttctga aatgatcttt tgggtttcaa caccaaaagc aaactgaatg ccagatgctt
     4261 ccatttgatg gacggcttcc tctaccaaag acgcctcaaa ttcttttaat ggccgttgat
     4321 tatgatggac aatggttact ttgctccctg cagcattggc aatcgtagct agctcaaaag
     4381 caatataacc accaccgata aaaatgattt ctttaggcag tacaggcaat gataaaaagt
     4441 ctgcactagt tttcaagtat tcctttccct ccactggtaa gatcgtaggt ctttgcccgg
     4501 ttgccaaaac taagtaatcc gcatggaaga cttcttcgtt gacttcaata gatgaatcat
     4561 ccaaaaaacg agcggttcct gaaagatgat cgatttcagc ttctgctaat tgtttttttc
     4621 ggctttctgg gactggatcc gtaaatgttc gtttgaattt ttgaagttct tcccagttgg
     4681 cagttgggat ctcgttaaaa ccttttccac tcaattgttt tacccgatta cgtgcctcta
     4741 ctgcacttaa caagactttt ttaggatcac atccacggtt aggacaagtc ccgccccata
     4801 gatcttcttc gaccactagt actgttttac ctgcttcttt caatccataa gcagcactta
     4861 agccgctcac acctgaaccg atgatgattg catcatatgt tttcatttcc ttcctcctct
     4921 cgacttaaaa aagcaaagat ttctttactt gtcagcgacc caatacttct ttgcttttct
     4981 actatcacaa tgcttggata caccgaaaga agcgaatata cttctttcat aggcgtatca
     5041 aagcaaactt gttgacgagc attttctggt atttcattta agaaatatcc attgacgact
     5101 aaatcttcta ctcgccactc tttcgtctgt ttcggttcat caaagaaatg ccgaacgtag
     5161 tcgtttttag gattcatcaa taactgctct ggtgtgtcac attggataat ttctccatcc
     5221 ttcataaatg caatacgatc acctagcttg attgcttctt ccatattgtg cgtaacgaaa
     5281 acgatggtcg tgcccaattc ttcgtgtaag gacaaaacca attcttgtaa tgagtttctt
     5341 gaaatcggat ctaatgcgct aaatggctcg tccatcaaaa tcacatctgg gcttgcagca
     5401 atagctcgga tgatcccgat ccgttgctgt tctcctccag acaactctct aggcatacga
     5461 tctcgataaa tatcaggaga taagccaact ttttgaagaa gttcatctac acggtctgct
     5521 cttttctgct tttcccaacc taacatttca gggatgactt caatattttg cttgactgtc
     5581 atggtcggga acaaagcgat ttgctgcagc acatatccca tattccaccg cattttctgc
     5641 agattatatt catttaacga ttttcctttg aagtagatgt ctcctgctga tggtttagat
     5701 aatccattga tcattttcaa agtagttgtt tttccactac cggaaggacc aaccaaaaca
     5761 aagatttctc cttttttgat tgccagattt aggtcatcga tgacatactt tccatcatac
     5821 tttttttgca catgtttgaa ctcgattaat ttagtcactg atcttcctcc tttactaatc
     5881 cttcattttt caggaagatt cgtgctactt ctgtcggctt tttcttttcg acattgactt
     5941 ggtaattcat atcaatcatt tgatcctctg ttatttttcc gcttaattta ttcagtgctt
     6001 taacgatttc aggatgttct ttagcaaaat cttttttcat cagcggtgcg ccttgataag
     6061 ctgggaataa ccctcgatca tcttctagag tcactagatc gtactccttg agctggctat
     6121 ctgtggaata ggcatccaca atattgacat ctccattatt gattgcttgg taacgcaaac
     6181 ttggctccat gctttgtaca cttgaaaaat tcaagccata tgcttcttgg attcctttat
     6241 atccatctga acgatcaata aattccagtg tgaatcccgc tttgatctga tcagagatag
     6301 ctgccaaatc ggaaatcttt ttaagttgat gttcgttcgc aaaagatctc ttgactgcca
     6361 aggcatacgt attttggtaa gccattggtt ttaataatgt catatcgaat tgcttattca
     6421 ataattcttc tgcttcgtca tatgtttttt cttttgtcaa atcagcactt ttcattgctt
     6481 caggtacctt caccaagctt tctaggacag tacctgtgaa ctcaggatag atatcgattt
     6541 gaccttgatc cagtgcattg aacaaaaaac tcgtcttgcc aaaattcggt tttaattcaa
     6601 cttctgtttt tgtgttgtct tcaatcaatt ctttatacat ttggatcagg atttcaggtt
     6661 ccgctcctaa ttttccagca atcgtgatgg tttcattttt aaacaaggca gactgatata
     6721 aagaaacgcc gccaaataaa cacaccactg ctgctaaagt aatgatggaa tatcgaggtt
     6781 tcttatgctg caaccagcga atcagtccgc tgaatataat tgcaagcaag gcagaactga
     6841 tcgcaccaat caatgtcaaa acaggtgtat ttcggtcaat acctaacaaa ataaatgtcc
     6901 ctaatccacc tgctccgatc aatgctgcca aagtagccgt accgatgatt aaaactaagg
     6961 cagttcggat acctgagata atgacaggta atgcgatggg aagctctact ttcagcaagc
     7021 gtctcattct ggacatacca aatgctaccg ctgcttcttc aatagacggg tcaatttcag
     7081 ctagaccaat atacgtgttt tggaaaatcg gaagcaaagc gtaaataaca agtgcaatca
     7141 atgctggaac agttccgatc cctacaaatg ggataagtaa acctaataag gcaagtgaag
     7201 gaatcgtttg gagcacaccc gtgatctgca ataagacttc tgccattttt ttatgattgg
     7261 ctgcccaaat cgctaaagga atagcaatca gtgctgcaat cagcaaagaa atgagagaaa
     7321 tggacagatg ttgaaatgca gcttccagca actcgccttt tctttcactt aatgtttgta
     7381 taaaattatt catgcgacct tcctttctgt tattctttaa gtataaacat ttctttcctt
     7441 tttcccaact aacttgtctt gccttaacct tacagcacga acaaaaaaaa gaaaaaaata
     7501 cctctaaata tgctgacaaa aaaagaatcc aagcaacaaa gtcggatcaa ctttttggtt
     7561 ctcgtttttt cactacgaat accataaaaa tctatacgaa attgtcactt ggaatcagct
     7621 atatcttttt tacacacaca acttttattt taataaaaag aaacagccaa taaagtagaa
     7681 atctcgtggg ggagaacacc tttattggcc tgttcaacga atattatact gtatcatgac
     7741 acttttttgc caaaaatgta aaaaatggtt gttttgacat cataaacgtc ttgttttctt
     7801 atttttactt atatcaccac tttattttct atctctttca ctccataaag aggaatgagt
     7861 tcttcttttg actgataata aacagctttc tcacggttct tcatcaaaaa aagaaagatg
     7921 tctttcatag caagtggtgc tttgccgtgt tcaaataaaa agataaattg atttgcttta
     7981 tttatctgga ccattgtcac aggcaccagt ttttgttctt gagccaaaaa aactccttga
     8041 tactctgttt ttttgggtga ttttgtaaga attgtcaata agtctgctgc tcgcattttt
     8101 ccactcctta tttgataaat aaaaagaaac agccaaaaat aatttttttt cattttcctc
     8161 ttgaagtaag agtataagtc agtataataa agaagttgct gaaaattttc aaaagaaaag
     8221 aggctagaaa tgaaaaaatt aaatttattt acagaattgt gcggaatcat tttaggcgca
     8281 ggaatctacg gattagctgt gacaggaatc aatattccat ctaagctggc agatggtggg
     8341 gttactggta ttgctttgct tttgcataat ctttttggat tcgctccttc tgtcacttca
     8401 ctgcttttta atttaccttt attgcttatc tcattgtttc tttttggaaa acacgctttt
     8461 ctccggacga tcgtcggaac atttgcgcta gttttctttt tacatttatg ggagagcctt
     8521 catatacatt ttgctgtaga tagtctactg atcaatagtt tgatgacagg tattctttct
     8581 ggcattggtt gcggtctcgt ctttcgattc ggcggaagca caggcggaac agatatcatt
     8641 tatcaagcca ttgaaaaata ttttcatatt aagattggca aaagtctttt cttgattact
     8701 tttggtattt tagttgtctc cttgctttat ttggatctca cacattttct gtatacatta
     8761 cttagctgtt ctattctatc ttatacattg aataaagtga agtatctgga aattggatct
     8821 gttttgcccc aattggaagc aaaacagact acagaaacat ttgaaccact tgatcctctt
     8881 gaacagttag agcactgatt cttatttttc actactttat ttaatttaca gaaatatcat
     8941 tatgtaaact aaaatgatat tattcagtaa aatcttcttt ataaaaaaat aaactatttt
     9001 tgcaaacgat ctgcgaaaat agtttgtttt tttattttgc cattattttc ttacttattg
     9061 ggagaaaaaa ttcgcttaaa ctcaaaaaaa tttgaggata cgatataaac tttcggatag
     9121 agtataaaat aaggaaacgc cgcagcgttt cctttccaac tgtagccgaa tataaatttg
     9181 tatatcactt atttaaaata gctattttct cccaaagagt ttccttgatc atcgatcaat
     9241 ccttgtaatg agcgttctaa gataccatgg ctttttttcg attttttttc ttctgactgc
     9301 tttgtttcct ctaacgtttt tgttcgattg aatgttcgta cagacggctc accatttctt
     9361 ttttcttgat acggcgtcgg ttctgtatcg aacaacagat aagagtcatc cgtttttttc
     9421 atcgattcga ttaaatgatc ctcagaaatt ttgctttctc tagggtctgg gataatcgat
     9481 gcgggtacat atttaggaac aaaataagaa cgctcccgca ccttcgtgtc aacagatgcg
     9541 tttcgtgtag tatgcttgga ctctgtcctt gtttcacttt gtctgacaga acgagaggaa
     9601 tcaccaaaaa gtttttttcg tcctgtcgat gtgatttctg attggtaatg gccggtataa
     9661 tccggtaatt ttcctttatg cttttccaaa ttttccttac gcggagctga cttcaatcca
     9721 tttgttgcac gacgcttgcg acggattttc tcggtgttcg tgttcgtgaa atgaaattct
     9781 ttcatctgtt caaacgggga ctgattctgt tcagtcaaaa gatcttcttg tccgtcaaat
     9841 aatactcgct tcttgtcgtt ggttaatttt acgcctaact catcatcgta cgcaggaaaa
     9901 cggaaatgtt ttcgtctgat ttctttcatt caaaccatcc ttctcaaaaa tggaactttt
     9961 ctcaataata gcacagattc attgttttca gtagcaatgc gctgattatt ttaagaaaat
    10021 agcgatgaat ttcataaata tatatatagt aataagactt aattcgttgt ctctctgata
    10081 actagttcag gaaaaaaacg acgataacct tgactcgccc ctttttcttc tatttttttt
    10141 aacaacattt ctccacatac ttcacccata cgtataatat cttgcttgat cgttgtcaat
    10201 ttaggtgaac tgatttggtc taaaaatacc ccatcaaatc caatcacacc aaattcttcg
    10261 ggcaagtgtc ctccagcatt tataatccct cttactgctc ctagtgctag ccgatctgaa
    10321 ctacatacaa aacaagtttt atctgtaaaa tctgcccagt tttctgtcat atatctttct
    10381 acttctgtcg aatgatttgg aaaccgcaag attttactag gaagatcaac ttccttcatc
    10441 acagataaat aaccagtttc ccttgaaatt tcaaaaggct cgtccacttc gataccgata
    10501 taaataatcg tttgataatc tctttctaag gcatattgcg ttgcttttgc tgtaccatat
    10561 tcattatcgc tgtctacata atcatatcct tcagaatttt ccccaaacaa gacaactggt
    10621 ttttccaaac ggtcgatcca atcgaagtct ttttttctga cacctgtgat gatgtatcca
    10681 tcacaagaac cgacgtcgaa ggcatcattt gtaactaatt ggagagaata atgcgcttta
    10741 ccgatccgtt tagcgattcc catcagcaaa ttcatgtaat agggctcagt tgtgtcgatt
    10801 tcttccagta taaataattt gatgatctgc gaccgattcg tgacaagtgc ttttgcagca
    10861 atatttggac ggtaatccag ttcagccatt gctgagaaaa ctaactcttt caattcgtca
    10921 gtcacctgtt ccggatgatt gatgactcga gagaccgtca tttttgaaac attggcacgt
    10981 tttgcaacat ctgctaaagt gggcatgctc tcttcctttc taacatgttg tcattcttac
    11041 tatcaattat accaaatttt cctaacagag tttattatag cccaaaataa aaaacggaaa
    11101 atcctctatc gtcatgagtg attttccgtt tcaattattt atactttttt atcaaatttt
    11161 ttgctaaaaa agtggaagct tcttccatta gtggtatttg taccatatgt cgctgtgttt
    11221 ttgaggaacg aaaaacaatt tcttttccgt aagacttatg ccgatttttt tctacaaatc
    11281 gttccgtttg tatataaggg attttttcgt cagcttgtcc atgccaaaag aacagaggac
    11341 gtccagctaa cttttccggc tgcaatgaca aatcatagct cttaacccat gaaatcaaat
    11401 caaaatagtc ttttggtacg acataattat attccgatgc gcgccttacg atttcttttc
    11461 catattccaa aggtgcaggg gaacccatca aacacacgcc cgctactata tgaggatgtt
    11521 tagccaacaa ggcgcatgtt gtcattcccc ccatcgaaac cccgccaact gcaagctgat
    11581 catgtagaag acctcttttt tctaaatgct tgataagtgt gtcaaattca aacagatttc
    11641 cgtagatact atcccaaaat gtataggatg gaatagaaga aaccggctgt ttgcgctccc
    11701 catgattcat cgcatctggc aggatcacac gaatgccctg ctgcgcaatt tttcttcctt
    11761 gcgtcaaaac taactcctta cttgtctgcc atccgtgata atagatgaca acgggaatac
    11821 gttcctctct taattctttc agaacaactt ctaacactgg aatatgatgg acagaacgtt
    11881 tcaatataat tggattcaat tgttactcac ctattttcat taaatttcct gatcgccata
    11941 gcaaccatgc tccaacagtt gtctggaaca ggttcagtgt accaaataat ataaatgcag
    12001 gactgaatgg ctcgggaaat aagcacagag aacaaagtcc caccactcct aatgctagcg
    12061 aaaggagtgg ataaatagaa aaattcaaaa aactggcaac gctaccgaaa atattaccaa
    12121 taaacagtat caaaaagaaa aaaacgctta aaattaaacc atctgtcagc gacatctgaa
    12181 aaacaatgtt ctctacctca attggctgtt gaatcgaata gacacctaag tatatactga
    12241 gaaggagact tagcacgtgg ctggcacaaa acacacgatt gaccgttttc attttaatca
    12301 cctaacctat ctttataatt ctattataat cattttatca tttttttgta tgtataaagt
    12361 ttcaattttt cttcacttac gatttttttt gatattgttg ttatataaat gaaaggagtt
    12421 ttacaaatga ttgatccaaa aatgttgggg atgatcttcc tcattaattt tgcttatatc
    12481 actctgaaca cgttacgttt catgttgaca atgaaaggct atcgactgat tgctccactt
    12541 gtcagtatgg tcgagatcac gatttatatc gtcggcttat cccttgtttt agatcgttta
    12601 gataatccac tgaatttatt tgtctatgcg ttaggttacg cagtaggtat cagtgtcgga
    12661 attttgattg aagacaaatt ggcattagga tacatcatgg tcacaacgat tttgccagca
    12721 aatagcgcag aagataaaag tttgccgggt atactgagag aacacggtta tggtgtgaca
    12781 cagacttctg caatggggct ggaaggtgag cggctagttc tcgaaatact ttctcctaga
    12841 aaaagcgaac gtgaactgta tcgcctgatc aaagaaatcg aagagcgtgc ttttatcatt
    12901 tcttatgagc caaaatatat ttcaggtggt ttctggacta aaaaagtgag aaaacgacaa
    12961 ggaaaataac tcacaaactt aaaaactgtc taatatctaa attaaatttt aaatgttaga
    13021 cagtttttat ttattatttt gattaaaaaa catcgacata tactcatcaa tttgttatta
    13081 tactttatat acttatcaat ttaatttgga gatattatgg aaattcaacg tagaaccact
    13141 aattttttac atttaggctc tttttttgca actttgacga tcttattttt tctatgttat
    13201 tcatacctta agacctcaca tgcgccttct agtgcattag gctggttgct tttggatcat
    13261 tctgatatca cagaagccga agaaaatccg ttttttttga ttttgtcttc tctttgcggc
    13321 ttactgtttt ctgtttattt cggtgcctat ttttttcatc gacatcctgg tacgttaaac
    13381 gaagcttcaa gcgtattgat tgcatttaaa acgttttctt ttttagcaat tcttttttat
    13441 tcgtttaatc acaatacgct tttcttcttt atcatgaatg gtttagtcgt gattttatcc
    13501 atttttactt attttatttt ttcactcatt tttagccaat tgaaatgtcc gctcttcttt
    13561 cgattagttc ctgtcacctg ttttatttac agcttgtttt ccttttttgt ccttgcttta
    13621 atgccactga ccaacccttc gacgatccaa gtcgcaggaa atctgttgga tatgctcttc
    13681 atcgtcagtc taggtgtatt tatgtggcaa agaaaaaaca aacaaaataa aaagcatttt
    13741 gaagcctgac tattacgact tcataaatgc ttttttagtt ttaaacactt caatctaaaa
    13801 gaataaccca agattggata aattattaat gactcgccat tcgtttctta gtgattctgg
    13861 attatcaaat ggacttccta atctaatcag ctagttcttc ccataaaatg ccatacttga
    13921 ctgaatcata atattgatta tttaaaaatc taacttttct tatcactact tcctttgtca
    13981 ttccacattt ctcaccattt ttttgcattc ctttgtttcc agaccatgta gtgaatctaa
    14041 taggtggtag ataggcaaaa tactcaaata attcttttaa ccataaagga agagaaaggc
    14101 tcctcctgca agagtcagta taatttttgc ttactgctct cttaggtttt actcactaaa
    14161 actgttccat attctttgat ataagaaaat gcatgcggta taaatttctt gctcagctca
    14221 gaaaagattt gtcgtttaag aaaggccatt tcctccattg tcaatgaagg atccaatgtt
    14281 ggtaattcaa tctcaaaatt gtttttttcg acaataggta attctaatgt gaatgattct
    14341 tcttccttta tagtattcgt cacaagtaga gactgatgga aaatactagg ccggcgattt
    14401 ttatatttat tcttacgtat cctgtcaacg ataacttcta tttgatcaaa aaagtacgat
    14461 tcatttacgt ctccaataag ttcggctaat tctttgaatg ccttcgttac aggaagctga
    14521 ttcttctgta caaaaaagta attagccaaa gtaagcggga agtgttctat agaggaaagt
    14581 gtgataaaac ggtacttctc atttcctgca gttgtcctca gcaattctgt ctctcggctg
    14641 tttcttactc cataaaaata cgcttctttc cgttcatctt cgaaacaata cgcccaaata
    14701 acttctccca tcgctaattt ttgttcatca acagagagtc tcttcaattg ttcagctatg
    14761 gtttcagcag ccgaagtggc ttgttgcaag taatctttag gatcaaatat tggaaagttc
    14821 aaatgatatc ttcgttcttc cctcttgatt aatcctgcat cgatcaataa atcaagaaaa
    14881 tgggaaaatt tattttgcga aaattctttt ttcaattctc ttagaatcgt ttcttgttct
    14941 cgatgttcat atagataatc ggtcaatgaa gaaaaaagcg gatcatcggt cagtttttga
    15001 taaaagtttt ccttattttt tgaataatag ttcatatatg ttctcttttc tatccgtttt
    15061 attttactta aaaggctttt acgtaaaaag gaggaagaac aagagtcata attgactgtt
    15121 attctcctcc atttcttcgg cctttagtat gttctatgtt tctttttata tcaatcgctt
    15181 atccattcat tttgaatgct tcatactacc atttgattag ctaaatacag ccgtcaattg
    15241 aggtacgact tgttttttac gtgaaacgac acctttaagc aaagcacggt tgttggtcaa
    15301 agttgtttgg aaagcttctt ctactttttc ttttccttta ccagcaacaa gtaattctga
    15361 atcactgtct aagatgtttg tgacaactaa tacgaacaga tcgtagccat ttgctttcat
    15421 ttcatcttcc attgctgctt ctagctctgc ttgtcgatcc atcacttcat taaggtccac
    15481 tgtattgact tgagcgatac gaacgttgga actgcccatt gggaaacttt ttgcatccaa
    15541 atcaagaaga accgcagctg atttatcact tagatttgtt cctgctttca acatttcaag
    15601 tccataagtg tttagatcga cttcagcgat tgctgcaagt tcttttgcag ctgcttcatc
    15661 ttcaggcgta catgttggtg atttgaacaa caatgtatct gaaatgatag ctgagagcat
    15721 gattcctgcg atttctttag gaattgttac ttggttttct ttatatagtt ttaaaataat
    15781 cgtacttgta catccaactg gttctgcacg atagtacaat ggattagctg tttcaaagtt
    15841 tgcaatacga tggtggtcta ctacagctaa aatatttact tcgctgatat ctgaaatact
    15901 ttgttggaat tcattatgat ccacaagcat gacatctgca ttttctgctt tagctgactc
    15961 tactactcgt ggtgcagata gctgaaagtg gtctaatgcg tattgtgttt cttcattagg
    16021 tgtacctaga gcaactgctt ctgtttcttt acctaattca ttttgcaagt gtgaaaacgc
    16081 aatggctgcc ccgattgcat ctgtatctgg attttggtga ccaaaaacta aaatttttga
    16141 catgtcattc gctcctttgt gatttctcct atttaataat agcaaaaaaa cggaaagaag
    16201 caatacttat cttctttccg atgcgaccta tcgcgttaaa tattttgtat actcttctgt
    16261 atgaagcaat cgtttagcat tttcgacacg ttcttttgta ggtggttcga tcccttctaa
    16321 tggatatttc aaaccaagtg tttcccactt gtattttccc atcgtatgat aaggaagaat
    16381 ctctactctg tctacatttg acaaggtttg aataaagttg tctagtcgga tcaaatattc
    16441 atcataatca cttcgctcag gaactagtac atggcgaatc caaaccggtt tgttgatttt
    16501 tgacaaatat tgagccattt ctaaaatatt ttcattgtcc caatgcgtta gttttttatg
    16561 ctcttcatta tcgatgtgtt tgatgtcaaa taacagtaaa tctgtatact tcatcaattc
    16621 ttgaaaacgt gaaaagaaag gttcttcata cgtaaaaggt tttccgcatg tatccaatgt
    16681 agtatgcatc ttccgctctt ttgctttttt aaatagttcg atcaaaaaat cgatctgcaa
    16741 tagaggttct ccaccactaa cagtaatacc acctttatct ccccagtaag gtctgaagcg
    16801 ttctgcttta tctaaaagtt catctgctgt gtattctttc cctccgccaa tattccaagt
    16861 atctggattg tgacaaaatt ggcaacgcat gcggcatcct tgcataaaca cgacaaagcg
    16921 cagtccaggt ccgtctactg aaccgaaagt ttcgatagaa tgaacatagc cgattgtttt
    16981 ttcttccatt taagttcaca tcttttcttt gatttcattt taagtacaag ttagctgtcg
    17041 aagcgaagct ttctgaacaa aatttttcgc aattgccgct tcgttatgtg taaaatttac
    17101 agggacggaa catacaagtc ctgcccctgt aaatcgtttc tttcaaataa gcatactgat
    17161 tattcgattt tgatgcgatg gctattcaac gtcctttcga cgctaagtcg aaaaactttt
    17221 ttcacgaact ttttacattc tgtcgtgtga agttctcatc aagacatcta attgttgttc
    17281 tcttgtcaag tcacggaatt taactgcata accagatacc cggatcgtca agttcggata
    17341 tttcattgga ttttccattg catcacgcaa taaatcatta ctgaatacat tgacattcaa
    17401 atgataagct ccttttgaga agtatccatc taagacatta cgcaagtttt cgattcttgt
    17461 atcttgttct tttccaagac ctgaaggatt gattgtttgg gtatttgaaa ttccatccaa
    17521 tgcacagccg taattcattt tagctgttga gttcaggcta gctaataacc cactttgttc
    17581 cgcattgtaa cttggatttg ctcctggcgc aagtggtttt ccagcttcac ggccatcagg
    17641 agtatttcct gtatttttac cgtaaacaac atttgaagta atcgtcaata atgaagttgt
    17701 tggttttgag ttgcggtaag ttttatgacg tttgatttgt cccatgaagt attccaagat
    17761 ccatgaaact aattcatctg cacggtcatc atcatttccg tatttaggga agtcgccatc
    17821 cacttcaaaa tcaactgcta ttcctttttc attacggatt gcttttactt ttgcatattt
    17881 gatcgctgac aagctgtctg ccgcatgaga gatcccagca atacctgtag caaatgtgcg
    17941 ttgcaagtca gaatccatca aagccagctg agctgcttca taagaatatt tatcatgcat
    18001 ataatgaatg atgttcaacg tattgacata tagttctgct aaccaatcca tcatattcat
    18061 gaagttatgt ttcacttctt caaagtctag tatttctgat gtgattggac gatattctgg
    18121 tccaacttgg gcgccagtca cttcatcgac tccgccattg atggcatata atagtgtttt
    18181 agccaagtta gcacgagcgc caaagaattg catgtctttt ccgacaactg ttcctgatac
    18241 acagcaagca atcgcacaat catcagaacc ccattgttca cgaagctgat tatcattttc
    18301 gaattgaatc gaagaacttt cgatagcgat tctgctagca tatgttctga atccttctgg
    18361 aaggtgttca gaatacaaaa cagtcaagtt tggttctggt gaaggtccca tatttgttaa
    18421 tgtatgaagc agacggaaat cattctttgt aactaatgaa cgaccatcta atcccatacc
    18481 tgcaatggat aaagtagccc agatagggaa tcctgagaat aattggttat attcaggtgt
    18541 acgcgcgaat ttgaccatac gtaatttcat tactaagtgg tcgatcaatt cttgtgcttc
    18601 tttttctgtg attactccat tatccaaatc acgttggata tagatatcaa ggaatgctga
    18661 gatacgtccg attgacatcg cagcaccatt ttgtgatttg attgctgcta aatatccgaa
    18721 gtatagccat tggacagctt cttttgcatt tgcagctggt ttagaaatat catagccata
    18781 tttcgcagcc atttctttca tttgcgctaa tgcacgatat tgttcagtga tttcttcacg
    18841 taaacgaatc acttcttcac tcattgtatg ataaccagtc atatcatgat cttttttctt
    18901 ttgttccatc aaatagtcga ttccatacaa tgccacacga cgatagtcac cgatgatacg
    18961 tccgcgaccg taagcgtctg gaagccctgt aatgattttg tttttacgag cagcacgcat
    19021 ttcagcagta taagcgtcaa atacaccttg attatgtgtt ttgcgccagt cagtgaaaat
    19081 atgtgtcaat tcatcgtttg gtgtatagcc attgcttttt agcgcattgt tagccatatt
    19141 gattccgccg aacggcatga atgcttgttt caaaggtttg tctgtctgta gaccgacgat
    19201 tttttccaaa tctttatcca aatagcctgc atcgtgtgaa gtaatagttg atacgatttc
    19261 tgtgtccata tcatagacac cgttacgctc gaattggata tcatttaact tttgcaattc
    19321 atcccataat ttttctgttg cttctgttgg gccttccaaa aagctgtcat caccgcgata
    19381 ttcagtgtag ttattttgaa taaagtctct agtgtctacg ccttctttcc atttggtacc
    19441 tttaaatcca ttccattgct ccattgtatg gacctcctaa atttctgttt tatgtaacag
    19501 tttttacgtg attagtataa cacagcaccc tatctttgca agggttttct tgttttttat
    19561 ttaacaattt ttatgtttta tttacatata tttaaatcaa cgttgacata ccaacattta
    19621 agcattgtga acactttcat ctttttttgc aaacgtataa ggatttatta aaaatgaatg
    19681 aaaaagagac gccacctgta ttaatacaga tggcgtctct tcattaaaat actacaacag
    19741 ttacgactcg atgaaaatct caggagcatc gataacttcg atgacttctc cacctttttt
    19801 ctcatccatc gcaaatgatc cattcgatgt tcgatcacct atagggaaat cagttgggtc
    19861 aatcgttaac tgctgccctt tttgattcat caaggttatt tcttttgatc ttccttccga
    19921 catatagata atacggtgcg gatttttttt cagctcacgc atgaccgtca ctccccgctt
    19981 cgcacgtgaa gtttggctga tttcttgagc aagcatgcgt ttaacagctc ctcgctgagt
    20041 aacgattacg atcggcgtat caccttctgc taacacaagc agcccgttga taatcctatc
    20101 tccatctttc aaattcatag acttcactcc agctgccttt gctccaacaa cgggaacatc
    20161 tgttaaagga tagcgcaaac ccattccttg atgcgttact aagaaaacat ctaagcctgg
    20221 ctgatcttct ttcagataca cagcaatcag ttcatcttct tccgccttta gcttcataca
    20281 gctgagcgga cggctcttat aggtgcgcca tggttcgaac tctgtcattc gtgtttgctt
    20341 gatcaatcca ttcttgctga tgaatacaaa tgttttctct gcttgaattt tttgatatgg
    20401 gaaaacagct aaaatcgatt cgtctacaga cagattggag attgtttgag aaatgtgctc
    20461 accagcatct ttccatttca aatcgggtag ttcatggaca gggcggtaga tcacatttgc
    20521 tttatttgtg atcagaagaa catggtccaa tgtactcaat tcacctgagt atagcaagta
    20581 gtcgccttct cgcatcccga tttcttctgg tttggaagca ctataagaac gaaggcttgt
    20641 tcgcttcaca tagccttctc gcgtcacact gacaattacg tcttcttgtg ccactaagac
    20701 ttgcgtatca attttgattt cttcgatttc gtcttcgatt gtcgttagac gagcggaaga
    20761 ataatttttt ttcacttctc gcaattcttt tttcataaca gagaaaagct ctttgtcatt
    20821 tgaaaggatt ttattcaact ctgtgatttg cgcaatcaag ctttcggatt ccttttgaag
    20881 ctctgtgata tccgtgttcg tcaaacggta taattgcaat gtgacaattg cttctgcttg
    20941 ttcttctgta aattgaaaga cgtcgactaa attctttttg gcgtcttttt tgtctttact
    21001 ttcacgaatc gttgcgatta cttcatccag aatcgataaa gctttcatca atccttctac
    21061 gatatgctgt cttttttgag ctttggctaa atcgaattga ctacgttttg ttatgacttg
    21121 ctttcgatgc tcgatataac ttcttaaaat gtctttcagt ccaacttgat gcggagtcat
    21181 atggtcgata gcgaccatat tgaaattgta attgatctgt agctcagtgt ttttgaacaa
    21241 ataattcaaa attccttgag cattggcgtc ttttttcaat tcgacgacaa tttgcagacc
    21301 tgttctatca ctttcatctc gtacttcagc tattccatcg attttcttat tcaaacggat
    21361 ctcatccatt tttttcacca aggtcgcttt atttacttca tatgggattt ctgtgatcac
    21421 gatttgctgt ttattaccct tcattgcctc tatctttgtt ttcgaacgaa gaataacttt
    21481 tcctcgtcct gtttcataag cttttttgat ttcatctttt ccttgcaaga tccctcctgt
    21541 tggaaagtca ggtccaggaa tatattccat caatttttca aggctcgctt gtggatgatc
    21601 gatcatatag attgtgccat cgatcacttc agctaagtta tgagtcggga tttctgttgc
    21661 atatccagca gatatacctg tcgatccatt gacgagaagg tttgggtatc gagctggaag
    21721 gactgttggt tctttttctg tatcatcaaa attccacacg agatcgactg tttctttctc
    21781 gatatctgct agcatctctc cacttaactt ggataatctg gcttcagtat aacgcatcgc
    21841 agcaggtgga tcaccgtcca tactcccgtt attcccgtgc atttcgatca gtacttcccg
    21901 caacttccag tcttggctca tgcggaccat cgcttcataa atactgatgt caccatgagg
    21961 atgataattc cccatgatat ttccgacaga ttttgctgat ttacggaatc ctttgtcata
    22021 cgtattgcca tctttgttca tggcaaataa tatccggcgt tgcactggct tcaatccatc
    22081 acggatatca ggcagtgcac gttcttggat aatatatttg gaataacgtc cgaagcggtc
    22141 acccatcact tcttctaaag ttaattcttg tacttcttgt cgattttcca tcaggtgacc
    22201 tcctattcta cttcaaataa ctgattgttt tcatttttat ctgctcgttc ttcatctaat
    22261 aaatcattag aaactgaagg agagatttca gtatcctctt ttttatctaa gatactgcca
    22321 tcttcttcga gcgtaaattg cacgtgattt tcgatccatt ttcttcgagg ttctactttg
    22381 tcacccatca gtgtggttac tcgacgttca gcttgcgcag catcatcgat acggacgcgg
    22441 atcaacgtac gggaagtcgg atccatcgtt gtttcccaca attgttccgc attcatctct
    22501 ccaagtcctt tatatcgctg cagcatatat cctttgccga cttttttaat cattgctgcc
    22561 aattcgtcat ctgtccacgc atattcaatg acttgcttct ttccttgtcc tttggatact
    22621 ttatataaag gcggtaacgc gatatatact ttcccagctt caatcagcgg tttcatataa
    22681 cgataaaaga aagtaagcag taatacttga atatgggcgc cgtctgtatc cgcatcggtc
    22741 atgatgatta ctttgtcgta attacagtct tcgatagaaa actcaggacc gactcctgca
    22801 ccaatcgtat agatcatcgt attgatttct tcatttttca agatatcttg cattttagct
    22861 ttttcggtat tgatgacttt tcctcgtaaa ggtagaattg cttggaactt tctgtctctt
    22921 ccttgtttag cagatccgcc agcagaatcc ccttcgacta aatacaattc atttttctta
    22981 gggtttcggg attgtgcggg tgtcaatttt cctgaaagaa gagattcgcc ttttttacgc
    23041 ttcttcccat ttcggctttc ttctctggct ttacgtgctg cttctctcgc ttctctggct
    23101 ttgattgctt tacggaccaa catctggctc atttcgctat tttcctgcaa ataaaagccc
    23161 atttgttcac tgatgacgtt atctactacg gtacgagcaa ctggcgtacc aagtttttct
    23221 tttgtttgcc cttcaaattg cagaagattt tcaggtacac ggatagaaag tacagcagct
    23281 aacccttctc tgaaatcact accttccaga ttcttgtccc gttctttcag caaaccaact
    23341 tttctcgcat attcattata agatttcgtc atcgccgctt tcataccagc ttcatgggtt
    23401 cccccatctt ttgtccgaac gttattgaca aaagataaaa cattttctga ataaccgtca
    23461 ttgtattgat aagcgacctc tacttcgata ccttcttttt ctcctgaaaa ataaacaact
    23521 ggtgtcaatg tatctttttc ttcattcaga taatcaacaa attctttgat tccttcttcg
    23581 taatgaaaaa tttccttgac tggttcttct ccacgaagat cagaaagaga gattttaacg
    23641 ccttttagaa gaaaggcaga ttctcttaac cgttcggcta aaatttcata tgaaaatttc
    23701 gttgtactga atattgtatc atctggtaaa aaatggacag tcgtcccgtt tgcttttttt
    23761 gtttttccta tctttttcaa agtaccgtca ggtttaccgc cgtttttaaa tttctgctga
    23821 tactctacgc catctctaac gatcgttaca attagccatt tcgataaggc atttaccaca
    23881 ctggctccta caccgtgcag tccaccagaa gttttgtacc ctccttgacc aaactttccg
    23941 ccagcatgca gcacagtaaa aatcacttca actgttggta ttccggaagc atgcattcct
    24001 acaggcatcc cacgtccgct atctgcaaca gttatactat tgtcttcatg gatggtcaca
    24061 tcaatctcac tgccataacc tgataatgct tcgtcaactg cattatcgac gatttcatag
    24121 actaaatgat gcaagcctcg gctgtcagtg gatccaatgt acatccctgg tctttttcta
    24181 actgcctcta atccttccaa tacttggatg gacgagtcat tgtattcgtt gtttactttt
    24241 tttgccaagg caaacgctcc ttcaataata ctttgcggca aaaccgcacc acgattatca
    24301 tactagaaaa ggtcgtgaaa aaaaagaggg tagacgagaa atcaatttta attatctcct
    24361 aaccgctttt tcgacattcc cctctatcga tcatctcttc tttgtggtca tttttatttt
    24421 tggttttctt tcaacttacc aagttcaatc ttaatacaac gatccattac gatatctgta
    24481 cgtcctgctt ttcttagcat ctcagctgct tctttgtttt caatccctaa ttgtgcccag
    24541 aacactttag catcagtttt caaaaaatca cctgctattt caggtaaaaa ttcactccgt
    24601 ctaaatacat ctacgatatc gattggatca tcgatttctg tcaaattcgc atagactttc
    24661 tcgcctagaa tttcttcacc agccaataat ggatttactg gtatgatttt atatccattg
    24721 tcttgcaata cctgtgcaac catgtaactt gttcgttctg gtttattgct caagcctacg
    24781 acggcaatat tttttgcttc ttccaaatag ttgaaaatcg tttcttgtgt tggatttttt
    24841 acagacattt ctgccacccc ttgcttttct ttttattcca tctattatac ttgtatcttt
    24901 cattttttca attgttcctg ttacctattc atgctaaaat aaaggatagt tttcaacttg
    24961 aaagagggta aaaaatgaaa ttgatcttat tacttatttt agcatatctt ttaggttcta
    25021 ttccttccgg tgtctggatt ggcaaattgt ttttcaaaaa agatatccga caacacggga
    25081 gcggtaatac tggaacgacc aatacatttc gtgtattagg caaaacagcc gggattgttg
    25141 tcctgcttat ggatatttta aaaggtacat tggctacttg tttgccgatg atcttccacc
    25201 tttctgggat taaccctctt tggtttgggg tatgcgccat tcttggccat acatttccga
    25261 tttttgctgg gttcaaagga ggaaaagctg ttgcgacaag tgcaggaatg cttttaggat
    25321 ttaatcctgt attttttgtt tattcttgta tcatctttat cgttagttta ttttgtacaa
    25381 gtatggtgag tttgagcagt atgatcagtg cagtattgat cactttatct acgatcgtcc
    25441 ttccttatgt agctcctgtg attttggcta agcccaattg gttactgacg atcattgcct
    25501 ttttagtttc ttcctttatc ttttatcggc ataaagacaa tatcaagcgt atcagaaacc
    25561 atactgaaag tcgaatcccg tttggtttga atagcagcaa gaaaaaatcc tgatactttt
    25621 tcattccaaa gagataaaac gggagtgtga caaatcgatg tcacactccc ttattactta
    25681 caggatagct tttttacaca ccttggtaaa cggtgttccc tgctaacttc tcggtcacaa
    25741 cctcttactt cactgtgatg gaatattttg tcttgaagat ttcatgggca cctagttgat
    25801 tgatccctac tttttccatc aagtttccac tactgttcac cgtgtcagcg attccgcacc
    25861 acggttcgat acaaacgaaa ggcgcttctt ttggataagg agaccagatc cctacataag
    25921 gcatatcttt atagcttaaa gttacgctat gggaagattt ttcacttcga atggcgtacg
    25981 catttagacc ccttgtttca aaaaccaagg catcttgctc aaaaagttca cgattcaaag
    26041 ccagattcgt atttgtctga cctaacgtct tttgatccat atcgatgaat gggcctttca
    26101 atggtaggcg taatcttgat ttcaatggag aaaatgccag atagaaatct tcaaatgaca
    26161 atccttcttc aagaggtaca ttgaaagcag gatgtccacc gatagaaaag aacatttctt
    26221 tttcaccggt atttgccact tgataatgaa ccgtgatgcc gtctcctcct aattcatagc
    26281 tgatcactaa ttcaaaatca aatggatact tttctaatgt ctccggcgta ctccttaaga
    26341 tgaaactagc catttcttgt ccatgttcaa tgacttcaaa atccatatcc cgagcaaatc
    26401 cgtgttgccc catttgatat gttttccctt gataaacata ctgatcatct tttaaacgtc
    26461 caacaaaagg gaataaaacc ggtgcatgtc tgccccaata cgctggatca ccttgccaga
    26521 tatactctaa accatgttct tttgatttca gactgatcaa ttccgcaccg tgttcagcga
    26581 tttcggcgat cagctggtca ttttgaataa taacagtcat tgccattcct ccaacttcga
    26641 cttacttttt aaaatgggaa ggctggaaca aacatttcta tctgccccag tccccttact
    26701 tctaataact gtgttttgct aactcgtctt taaaacggga attcaatact tttaaataag
    26761 ttcccttcat gcctaacgaa cgagattcaa tgattccagc tgattccaat tttcgtaaag
    26821 cattgacgat cacagaacga gtgatgccga tactatcggc aatattagaa gcagtcagac
    26881 gaccttcatc tccatccaat gcttcaaaga tagcttgtac agccttcaat tcgctataag
    26941 ataatgtgtt gattgccatt tggacagccg ttgcactccg tacatctgct tcgatgcttc
    27001 gagacttctg ataaaggatc tgcataccga caaccgtagc tccatattct gctaagacta
    27061 gatcgtcatc tgcaaacgat tgttccatac gagataagat aatcgtaccc aaacgttcgc
    27121 cagctccaaa gatcggaacg acagtcgtca gaccaaatgg atatttctct cttgactcta
    27181 ccggaaatgc agtaatatca ctagtaattg ggatattagc ttcagttttc atcaactgtg
    27241 ccactgtatc agtataagat tcaggaaatt gtttctcaac aaacattgtt ttgattcgtt
    27301 cattgttcac atcgtgacgt tcattataac ctagtaatac tccgtcactt cctatgatat
    27361 aggtgttgct gtcaagaata ccacctagtg tgacagccat tttatcgtaa ggcaaatctg
    27421 cttgcatgtc aaaagtattc ttttgctgca aaagtgtgtt tatcttgcgg gttttcgtta
    27481 gcaagtctgt cattgtcgtc ctccgatttt ttataaaata tagcgactta aatcttcatt
    27541 tgccgcaatt gagccaagtt tgtcatccac atatgcttct gtgatcgtta tttctcccat
    27601 ttgcatatct ggtgcttcaa ataatagatc ttctagtagt ttttccaaaa tggtatgcag
    27661 tctgcgtgcg ccgatattat ctgtgtcacg gttgacatcg aaagcgattt gagcgatccg
    27721 ttcaattgct tcttttgtaa acgtaactga cacattttct gtaccgatca acgcgacgta
    27781 ttgttttact aaagcattat ttggttcggt cagaatcttc acaaaatctt cagctgtcaa
    27841 atcatctaat tctacccgta tcgggaagcg tccttgaagt tctggaatta gatcacttgg
    27901 tttagacaaa tggaaagctc ctgaagcaat gaataggacg tgatctgttt ggatgatccc
    27961 gtattttgtg tttacttgtg aaccttcgac aattggcaat atgtctcttt ggacgccttc
    28021 tctggatact tctccagatt gctgtgattt agaagtgatc ttatcgattt catcgataaa
    28081 aataattcca ctttcttccg ctaatttgat tgcctcacta tggatatcag catccttgac
    28141 taattttgct gattcttcct tgatcaataa ctctaaagct tctttgactg ttaccgtccg
    28201 ttccactttt ttcttcggtg tcaaagcacc tagagtttca tttaggtcga tccccatttg
    28261 ctccaatcca ttgttcatca ttggtgcagg tttcttttgg tcttcaactt caatggttac
    28321 ttcacgacta tccagtaaac ccttttgtag ttgttccaag actgtacggc gattgattcg
    28381 aatatcttct gtcacttctt cttgcggttc agcggtattt tgctgactag cattaaaaag
    28441 attcatcatt tgttcaaatt gattagtgga ttgtttctgt tcttttttga taccaggtac
    28501 caagattttt accaaacgtt tttctgcatt ttttttagca cttgtgtata cacggctgta
    28561 ctgctctttt tctacgattt ggatcgcatt ttctactaaa tcccgaacca ttgattctac
    28621 atctcttccg acataaccga cttcagtaaa ttttgttgct tcgactttga caaatggtgc
    28681 attgacgatt tttgccaaac gtcgtgcgat ttctgtttta ccgacccctg taggtccgat
    28741 catcagcata ttttttggtg taatatcttg ttgcattttt tcatctaact gcaaacggcg
    28801 atagcggtta cgcaaagcaa cggcaacaga tttttttgct gcgtgctgtc ctacgatata
    28861 ttcatctaat tcacttacga tttgtcttgg tgtttgtttt aattcataca tatctactca
    28921 cctcttacag ttcttctacg ataatattat gattagtaaa gatacagata tctgcggcaa
    28981 tatttaacgc attttctgca atttctttcg cactcatgtc gttttgagca tagtttttca
    29041 ttgctcgggc tgcagccaac gcaaaattcc ctcctgatcc gatagctaag ataccatcat
    29101 ctggtgtgat aacttctcct gtacctgaaa caagaagcat ttctttatca ttcattacga
    29161 tcagcatcgc ttcaagtttt tgcatagatt gctgtgtccg ccattcttga gctaattcga
    29221 cagctgcgcg ttgtagattc ccgttatatt cgtttagttt gccttcaaat ttttcttcta
    29281 aagtaaacgc atctgcaaca cttccggcga aacctacaac aacttctcca ttgtagatac
    29341 gtcgtacttt ctttgcagtg cctttcatga caacagattc acccattgtt acttggccat
    29401 ctccagccat agcaaatttc ccgtcttttt cgacggcaca gatagtggtt gaatgaaatg
    29461 gtgattcgac catgtttgtt tcctcctaaa tttgttcttt tccttatttt tacgctcttg
    29521 gatggaaaga acgatagttt ttttgtaaac tttcttttgt tacatgggca tagatctggg
    29581 tagtcgataa attggcatgt cctagcaatt cttggactgt ccgcaaatct gccccattat
    29641 ttaataaatg agtagcaaat gtatggcgca gcatgtgagg atggatatca ctatccaaac
    29701 tgcttttttt gatcatttga ttcaagacat attcaattcc agtaggcgtg attggttttc
    29761 catgatgatt cacaaaaacg atatcatgct cttcgtgata ttttgtcatc aatacctggc
    29821 ggccttccct gaaatattct tgcagagcat cttgagcaaa agaacctaga ggagcatatc
    29881 tctctttgtt ccctttacca tggatcaaca tgacctctga atcaaaatca atgtctgata
    29941 ccgacaaact gctacactca cttaaacgga tgcctgaccc gtaaaggact tccagtaaag
    30001 cgcggttcct caaatccaaa ggactatctc ctgctacgct gtcaaaaagt gcttgcattt
    30061 cattttcata aaaaaaacgt ggcagtctca aatttttctt tttgagatga acgtaagaaa
    30121 atggattttc ttttatcact tcttctttga gaagatactg ataaaacgaa cgtaggctgg
    30181 ctatcttgcg actgatagaa tttctgctat agctaggacg atgatcagct aatgcactca
    30241 aatagacacg aaccgtaaaa tattccgcct tcagcagatc agcgtctcca gattcctcca
    30301 aaaaatgttt gaaatggaga atgtcttctt catatgcttc tttcgttttt tctgaatatc
    30361 ctctttcgac aatcaaataa cgcaaaaatt tttgttccca cgtggcttct gtcattttct
    30421 caccttcctt ttgtcccaga gaataattta gcacagcgtt tataaaaaga caaataaatt
    30481 gtcagaattt aaccaatatt tctaagtttg ttcacaattg gcgcaattcc tcacattttg
    30541 tttgagaaat tcttaagaaa aacgcaagaa aaagagcgag actatccgtc tcaccctctt
    30601 catggagttt ccttactcat ttgggattgc agttttttct aattctgttt ttattgcctc
    30661 attagctttt aaagctcgtt gtgcaattgc ttcatatctt gcttttttgt ctcgaatgcg
    30721 ctctggcagt tcaggaaaca gaccatagtt ggcattcatc ggttggaaat gttttccttc
    30781 cgcatgagta atgtagtaag ccatacttcc aatagctgtt tcttgtggaa actcgactgg
    30841 ttccatttct tttgctaagc gtgcggcgtt gattccagca agtagtccgc tcgctgcact
    30901 ttcaacatag ccttctacac cagtcatctg acctgcaaag aacagatctt cgcgtttcgt
    30961 tgattggtaa gtcggtttca gcaattctgg cgaattcata aagctgtttc ggtgcatcac
    31021 accatagcga acgaattcag cattttctaa cccaggaatc atccggaaaa cacgtttttg
    31081 ctcgccccat ttcaagtgag tctgaaagcc cacgatgttg tataaagaag ctgcagcatt
    31141 gtcttgacgc agctggataa cagcataagg acgtttgcca gttttaggat cttccaatcc
    31201 aactggtttc ataggtccga aaagcatcgt tttgaatcct cgctgtgcca ttacttcaat
    31261 tggcatacat ccttcaaaat atttttcttt ctcaaaagat ttaagttctg ccacttcggc
    31321 attgattagt gcctcatgga aagctttgaa ttcctcttct gtcatcggac agttcaagta
    31381 agctgcttct cctttgtcat agcgggactt caaatatact ttatccatat cgatcgtagc
    31441 tttgtctacg attggtgcag ctgcatcata gaaataaaaa ccatctgaac cattgaatgc
    31501 tttgatgctt tctgctaatg gatgagaagt caaaggtcct gttgctacta cggtgattcc
    31561 ttccggtatt tcagtaattt cttcgtttat cactgttact aatggatgct ctttgacttt
    31621 tcgggtaatt gtttcagaaa aagaatcgcg gtcaacagct aaggcaccac cagctggcac
    31681 tgctgtttca tctgccgaag tgattactac cgaattcaat cggcgcattt cttctttcaa
    31741 taaccctact gcattagcca aactgtttcc tcttaatgaa ttggaacata ctagttcagc
    31801 aaaattactt gtgtgatgcg cctcagtcga tttgactgga cgcatttcat ataaacggac
    31861 agggactcct gcttgtgcga tctgccaagc agcttcactt ccggcaagcc ctgctccaac
    31921 aacatttacc attttcaatg aaagaacctc ttttctatta tttttggatg tcttcttcgt
    31981 aatctccatt tacacagact acttgtttac cgcctttgac tttcttctcg accagatatc
    32041 cgtcacattt tggacaattt ctgcccactg gtttatccca agaagtgaat tcacattctg
    32101 ggaaacggga acaaccatag aatagtctgt ttttcttaga ttttcgttca atgacttgtc
    32161 cttcatgaca tactgggcaa gtcacaccga tttcttttac gattggtttc gtattccgac
    32221 aatcaggaaa attactgcaa gcataaaact tcccatattt ccctaatttt atcaccatcg
    32281 gatgtccaca gacatcacaa tcgaatcctg ctggttcatc tttgatttga attttttcga
    32341 ttttttcttc cgcattcgtc agttcttttt caaacggttt atagaaacga tcaaccactt
    32401 tgacccacgc ttccgtgcct tcttcaattt tatccaaatc tccttccatt tctgctgtga
    32461 aatggatatc gacgatttga gggaagaatt cacagattaa tgaattgaca atttctccta
    32521 gttcagtcgg ctcaaatctt ttttgtgtca attttacata gtagcgtctt tggatcgtat
    32581 caagtgttgg tgcataagtg gaaggtcgcc ctaccccgtt ttcttctagt gcacgaatca
    32641 acgtagcctc actaaaacgt gctggtggtt gagtaaaatg ttgttttggt tcaatatcaa
    32701 cagattggac tacatcgcct tcttcaagtt ctggaagaat attttctttc tcttctttgc
    32761 cgtcatctct accttccaca tacacttgca tgaatccttt gaatttgatt tttgaaccgt
    32821 tcgcaatgaa gatgacacca ttttgttcta atgtcacttt cattgtatct aaaatagctg
    32881 gtgtcatctg actagccaca aaacgtgacc agatcaatgt ataaagtttc aattgatctt
    32941 tatctaaata ttgtttgagt tcatctggtg tacgaagcac actcgttgga cgcacagctt
    33001 catgggcatc ttgtgcgcct tgagcatttt tgatctttcg gtgatcatgt ctagaaaatt
    33061 cttttccata cgtatcttca ataaatgctg cggcctctgc ttttgctgta tcggcaatcc
    33121 gggtagaatc agtacgcatg tatgtaatca agccgactgt tccttgttta cccaaagtga
    33181 ttccttcata taattgctgt gctaccatca tggtttttct tgttcgaaag tttaacttac
    33241 gggcagcttc ttgttgcagg ctacttgtag tgaaaggatt cgcagggttt cttttgcgct
    33301 ctttcttttc tactttttta acttcgaagt ctgttccatc aattctagca gtgacttctt
    33361 ttacactttg tgcatctggt aatttctttt tcttaccgtc gattccccag aaatttgctt
    33421 tgaatttctt gcgagctttt ttaaaattac cgtcgatgct ccaatattct tctggaacaa
    33481 attctcggat ctctttttca cggtcaataa taatttttaa agcgacagat tggacacgtc
    33541 ctgcacttaa ccctttcttg accttacgcc agagaatagg actgatcgaa taaccaacga
    33601 ttctgtctaa ggtacgacga gcttgctgtg catctactaa atctacatcg atcgttcttg
    33661 gttccttaaa agctgctttg actgcgtctt ttgtgatttc attgaagacc acccgatttt
    33721 tttctttcag atccaaccca agtaggtacg ctaaatgcca agcaatcgct tctccttctc
    33781 tatccgggtc acttgccaag taaacttttt gagctttttt tgctgcggct ttcaggcttt
    33841 tgatgacatc gccttttccg cgtatagaaa tataatgggg ttcataattg ttttcaaaat
    33901 cgatacccat tttacttttc ggtaaatctc ttatatggcc aacactggcc attactttat
    33961 agtttcgtcc aagatatttc tcaattgtct tggcttttgc aggtgattct acaatcacta
    34021 aatatttata cgccattact gaatggactc ctttccattg tcgtgttcct actattaaaa
    34081 gaggattcat acaaatcgta gctaatcata tccattcaga aaacgtttgt caagaaaagc
    34141 aaatgaaaag tatggtgtta ttctttaaat tctgccaaga tatcttcacc cgaaaccgtg
    34201 caaactgcac cgtcttggat caaatgatga catccggtag aatgttctgt taaaatattt
    34261 cctggaacag aaaaaactgt tcttccaaga tccaatgctt gctgtgctgt tatcagagag
    34321 ccgctttttt tcttggcttc aaagacacat gttcctttag acaaagctgc gataatccga
    34381 tttctcattg gaaaacaatg ctttttcggg ccggattgag gaggatattc actcaaaaga
    34441 agatgatcat ttgcaatcac ctcttgcaag tcacgatttt cagcaggata gacttgctct
    34501 atccctgttc caatgactgc gattgtccgt ccaccatgag agattgctgc ttggtgaccg
    34561 tatgtatcta tccctttcgc gcagccactg acgatcacat atccagcttc tatcactggt
    34621 tttagtaaat aataaactgt ttttttggct aaagcagaag tgtgtcttcc accaacaacg
    34681 cttaacatgt tacaagaaag taaagataga tcaccattat aaaacagtac aattggcgga
    34741 taggtcaagt gaagcagctg agggggatat ttgggatcaa agcaagtgat aaacgactgt
    34801 ttcgattgga ttttgcccca gttagcgtga atttcctgcc aacttttgcg aaacagctcc
    34861 cggtgtttgg ttattttacc tatcttaatg atgtcttcaa atccgatttc tttataagga
    34921 tactgcatca tccagttagc aagtgcccat tttccttgac tactgattcc ttttgtataa
    34981 gcaagtttca ataaattttc ttctatttga tacatcataa aaccgccttt cgtctctctt
    35041 tctaataaga tacgtaaaag cggctgtttt ttattgtaaa tattttttta caggtgcaaa
    35101 acttttacga tggattggcg taattccttg tcgttcaagt ccttctaagt gttcctttgt
    35161 tccataacct gcattatgcg agaaaccata tccaggataa agcacgtcat attctttcat
    35221 caattggtct cgatatactt tagcaatgat acttgcagca gcaatcgaca cagatcgtgc
    35281 atcccctttg attatttttt cctgctgaat aggcaaatcc aaggtcatcg catcgatcaa
    35341 caaatagctt ggtacaatgg acaatttttc tactgcttgg atcatcgcta atttacttgc
    35401 ttcataaata ttcacatcat cgattgtctg ctgatcaacg atcccgatcc cgattgccag
    35461 ggctttttct tgaacttgtt gataaagaag ttctcgcttt ttctctgata attgtttgga
    35521 gtcattcagc cctaatatga tctcattttt cggcaagata actgcagctg ccacgacagg
    35581 tccagctaat ggtcctcttc ctacttcatc aatgccggca atccattggt gaccttgtgc
    35641 gtacccttgc ttttcaaagc aaaacatttc ttcatatttt tccttaatta atgcttcctt
    35701 tttcaatcgt cgttcaaaag aagccagtgc ttgctgcacg cctttgcgtg gatcatttct
    35761 taatgattct agtgtaggat catctattct ctgtatgctt ttcaatcgtt ctttgatact
    35821 ttggatggat tcagtcatca cttttttgct ccatttcttc gcaacgatcc agtgtgtatc
    35881 gtccaagttt tccttgacga atatcatgca cgactaattc gctgccacga tcatagtctt
    35941 ctttataccc acgttttgct gtaatatcca tcagtaattc cggtgccaat ttttcttctt
    36001 gctcgattga taaaccataa cgatcgctga ttcttcctga ataatatcgg gcaaaaaagt
    36061 ctaatccgta aatcgctaaa tcatctaaat ggacaagctg gtctttgatc gcgcctgtca
    36121 gcgctagttt tttgccgatt tcttcatctt caaatttggg ccataaaatc cctggtgtgt
    36181 ctaacaattc caactcatta cctaaacgaa gccactgctg accttttgtt acgccaggct
    36241 tatttcctgt ctgtgcaatt ttcttgccta ctagtcgatt catcaacgta gatttcccaa
    36301 catttggaat acctaagacc attgcccgaa tagcgcgagg cttgatcccc cgagcagcat
    36361 ctcttgcccg tttttctttc aatgcttctt tagccgcagg tactagacct ttaattcctt
    36421 tactttcttg tgccgtgata gctaatgcaa gatggccttg tttacgaaaa tattcttgcc
    36481 acagctttgt ctgttgtgga tctgccagat ccgctttatt caataaaaca aggcgaggtt
    36541 tttgctgaat gatctgatct agcattggat tacgagaaga taaaggtaat cgtgcgtcta
    36601 ccaattcaaa tacgatgtcc acatatttca ttttttcaga aacttctcgt cgggcttttg
    36661 ccatatgtcc cggaaaccat tggatggtca ttgttctctc cattcctttc ataccggaat
    36721 attccccggc tttacttgtt tttttctttt tcaatataaa ttcacttttt tattataaag
    36781 aaattccatc tattagttaa gtgaaagtga accagtcctc attctatcac atcttcactt
    36841 ttttgatgat gcaaattttt attttggttt ctatatatga atctaaattt ttgacagcgt
    36901 ttcctaaata agagatgata gacgaacaaa acagaaagga gacgaaacaa atgaatagta
    36961 tggtttttgt taattttcct gttgctgatg tcaatcgttc cgttgctttt tatgaaaagc
    37021 tgggttttaa aaaaaatgaa gaattttcta ctgaagaagc tggttcaatg gtatgggatg
    37081 acactttttg gataatgctt ctctctcatg atttttataa aaaatttata aaagataaag
    37141 aaatagctga taataaaaaa acaagtggtg ctttgatttc gtttagtgtt gaaaatgcgg
    37201 atgaagtaaa aaaatttgct caaattgcaa aagaaaatgg tggtactttc cacgaagtag
    37261 atatgggaat gcctgaagaa gaaatgtttt cattagaagt ccaagatcta gacggaaata
    37321 cattagaacc cgtatggatg aagatgtaag cagataacta atctgacgtt ttttataaca
    37381 aaaaaacaaa ggcggatgat acgataagta ttctccgcct ctattttttt tattcgtaca
    37441 atctcttttt tacagtttat aaaagcagtc ttctcaccct ttttttaatt gttagatgcc
    37501 agtgtatgta aatcaccaat gtgtcatgac acattggtga ttttatggta tccttttatt
    37561 attaactgac aaaggagaga atattttgaa cactttgatt gcaaaagacg tttttagatt
    37621 gcccgaaatg aatgcaagat tgttgtcgac ctctgctctt ttattagccc ttgatgtcat
    37681 cttatacaag atagcgattg gtcctgctta ttttcaaatc acattcggat ttttaagtat
    37741 ggcgcttatg ggttatctat atggaccgat ctgggctggt ttgcttgaaa tgttgtcgaa
    37801 cactttaaat ttcacgatat ttggttcagg aacgtttcaa gctgcttttc ttatcacggc
    37861 tttccttggt ggatttatta atggcttatt tttatatcat aaaaagataa acctgccttc
    37921 cgtcatcgct gcccaattat tgattatgat cccagtcagc ctttttatca attcttggct
    37981 gatgtctgtt ttgtttggtg cagatttcaa agtaattttt ctaggtagat tcatcaggaa
    38041 tatgatccaa atccctattc aaataatggt tgtttattca tttatcaaaa ctttagaggc
    38101 tagaaaagtg tttaaacgag ctgtttgatt ttacgtacta aggtagtaaa ctgattttgg
    38161 attaaacaat aaagcgtgca gtctcaacag actgcacgct ttattgtttc tcactggatc
    38221 cattttattt catcaagtgg ataatagaca aatcttgctt tacctagaat atcagtttta
    38281 ttgatcgtgc caaacgaacg actatctttt gaaactcttc gattatctcc aagtacaaaa
    38341 taattatctt ccggaagctt ggattgtccc gttagctctt gtagtgtaaa gtccgtcgta
    38401 taggatgcat ttgcatgatc tgatttcagg tttttagtta gataaggttc cttaactact
    38461 ttcccattga tcttcaattg atcattttgg tagctgacat tttctcctgg aagaccgatt
    38521 actcgtttga tataaatcgt gccatctgct aattgaaaaa caacaatatc aaatcgtctg
    38581 atctccgaaa atttttccat cacaaccata tcaccttgct tcaacacgtt ttccattgaa
    38641 ttgccttcca ctggtaccgg gatgaataaa aatccccgca acatgaacgc aaggaataca
    38701 gaaacaagta agtatttgaa tacaagccag aagcgattaa tatagcgttg tttttttgtc
    38761 aatctctatc aatccatccc tctttttttc attctttctt tattataaga aaaatatcaa
    38821 aaaaatgcga ctagatagct gaaataaaaa taattctttc aattaggcta ttggacaaaa
    38881 aacttttttg atcataaagt acaaaactat ctcttatatg tcatgattat tttctaaata
    38941 tttttcccat gcttcgtcaa agatatccat ggacggcaaa tagtcgacat tcagttccag
    39001 ataattagaa atttcttcgt agctagagct gtgcttagga aattggatgt ctttaccggc
    39061 ttctgtagcg aaaacagata aagcatcatg tgtgccaccg cctcgcagtg tcattaaata
    39121 atgaaaaaag cttcgattca cccttcgttc ctccaatttt cttcgataaa ggcagcccgg
    39181 cgtgcatggt ttcttttaaa attttcagga tgctttttat aatactcttg atggtcttct
    39241 tctgctagat aaaatggttg ggctggttcg atttttgtaa cgattggctg ttcgaaacgt
    39301 ccactttcag ctaactgttt cttacttttt tcagcaatga tccgctgttc ttctgtcaaa
    39361 taaaagatca ccgggcgata attgtcaccg cgatcctcga attgtccgaa agcgtcagtt
    39421 ggatcagttt gctgccagta aagttctact aattgttcat atgaaatcaa tgttggatca
    39481 tagataatct ccactgcttc tgtatgtcca gtcgtttggc tttttacttg ttcataagtc
    39541 ggatctggta catgccctcc agtatatcct gacatcactg tatggatacc aggaagcgtg
    39601 tcgaaaggct ggatcataca ccagaagcag cctcctgcaa atattgctcg ttccattatg
    39661 attccccctc taatactact catccgatga ttttttattt tcttttgtct tgctttcagt
    39721 actgctggtg ctagttttgt tggtatttga gttgtttgaa gcatcagagg tatcttttgg
    39781 caaatataga ctaaaccgaa tgtcatcatt tactaagtcg attttatctg ctttaatata
    39841 catcccgttt tgcattcgga attgatccaa cctaagcatg atagtcagat catctgtatt
    39901 gacttcgatc caatctggta atttatagtt tctcttgatc atattcatca catctttgat
    39961 tggcaaactc aatgttccga tagaaagact tctagctttt agttggacat ttccgttttc
    40021 catcacatac ggatcaaaat ataaatagaa atcgatagga aaacctagta cttcgaaagt
    40081 accattgagt agtgcttcat tttccaaata aaatttatat tcaatatcac tatctttttg
    40141 atactcacta aggaaaaagc tgatgatttg attgactttt tctttatttg aattgatcgt
    40201 taagacagga tctccttgtc tttccgtaag agctggtatt tctgggagtt ctggttcccg
    40261 attagcaaaa atacggctgc ctaaaaatac ggtcgaacca agtactaccc caagaaggat
    40321 aaggaaggcg aatttccagt aatttgtttt ctttgttttg tttgaagata cgtcagatct
    40381 tgttttcatg tttattgatc atcctccttc tctaaccatt ggttttgtgt ctcgatcagc
    40441 tgatctctca ctgcatttgc catcaattga taacctaaat tatttggatg gaaatgatct
    40501 tcttcataga gcacattatt tttgatgcca tttagatttt ctgtcgtgct agttgtttga
    40561 tctgttcctt cttctgtgac acctacttcc tgattcaatc cttgataaag caaatcattg
    40621 attgggataa agtagcagtt cggcattttt tttgtcaccg tttctgttcc tttgttccaa
    40681 ttgttaacaa ttgtctgcat gtcagtgatc tcaggaaagt tcaaataaaa tggattgtag
    40741 atgcctaaaa tgtaaatagg tgcggtttta ttgagggatc gaaattcttc caatagttct
    40801 gtgacatttt cctgatagtt tttcagtggc tttttaaagg aattgaccgt taacccgaat
    40861 aaattatttt ggataacttt catcaaatca ttaccgccaa cagtcagtgt gataaaatca
    40921 gctgttttga tattgtttcg aatttcttct tttgttttta ctcgctttaa gatctggtca
    40981 cttcgttcac catttacacc atagttttct gtttcaaccg cggtcaaatt ataacggtct
    41041 tttagatcat ttgcgacaag gggaacgaat cctccttgct tggtttgatc tcctactcct
    41101 tcagtcagcg aatctccgat agctatgtag tgaagttttt cttttttatt tggatcttct
    41161 ctcacagtag ttactggttt cattagtggg ccagctttcg gtatagaagc agctaaacct
    41221 gcatagatga tgatcgcaat taataatggg aacaaaacga ggatcaaatg tcgtttcgtt
    41281 aatttcattt tttcacctct tgtattcgat tggttctaat gttatcaaaa attttatcaa
    41341 aaaggtagtc tttatgaaaa aagaaaacaa ttattttcct aattttataa agaaaaagag
    41401 aataagctgc aagcctttca ggctgagggc ttatcctctt ttcgactaat ctacataata
    41461 catgattgca aacgcgtctt taccagtatg cgtagcgata accggattcg tatgcagtac
    41521 agggatatgc atgtccggga ataattcttg caaaccctct ttgaattctt gcgccaattc
    41581 ttttgcatcc gcatgtgaaa taccgatttg tcgcacattt ggcaattctt tcagttcatt
    41641 tttcaaatta ttaaaccatt tatcgaacgt tttctttcct cgccccttag ttacagggat
    41701 taattcagaa tgggcgaaat tcatcaccac gcgtatgttc aaaacactag acagcaaacc
    41761 agttgttcgg ctgattcttc caccttttac caagttatct agtgtggata ttccgatgta
    41821 taattttgta ttgtcttgta cttttttgac ttcttcaagg attttctcta ctgttttacc
    41881 ttcttttgct aattcagctg ctcgaattac ctgaaaagat aatccttggt cagtggtatc
    41941 actgtcgata accgttactt tcgtttttgt tagattactg gcttgtcttg ctgcttctac
    42001 tgttccactc aagccttttg tcatatggat cgaaatcact tcactgccgt cttctcctag
    42061 ctcatcatac atcgagatga attctccaat tggtggttgg cttgttttgg gcagattttt
    42121 tgctttcgcc atcatttcca taaatctttc gccgtctagt tggtcatcat cgggatatac
    42181 tgttccgtcg atcatgatgg atagcggcat gacacgaatg cctaaagcat cgcgtacatt
    42241 tttttccatc gtacatgaag aatctgttac gatttttaca tttgtcatta ttttctctct
    42301 ctctttcttt aacaagatgc cttattcatg ttaaactaca tgtaagacaa tttctatttt
    42361 tagtataaca gacaaataat gatttttcat taattacacc tagtaaagga gccgttttca
    42421 tggaaaaaac gaaatttaat cgaacgtatg aaatattaaa tgaagtgttt aatgcagtca
    42481 cccatggaat tgggactgga ttgagtatcg cgggactagt ggttctttta gtaaaaggtg
    42541 cccacttgca ttcctccctc catattgtct cttatgctat ctatggatcg atgatgattc
    42601 ttttattttt aacgtctact ttgtttcatt cattgatttt tacaaaagca aaaaaggtgt
    42661 tccaagtatt cgatcatagt tcgatttttc ttttgattgc aggaagttat actccttttt
    42721 gtttgctgag tatccgagga tggctaggtt ggactttatt tgtattaatc tggttgtttg
    42781 cgattcttgg cattatatat aaagcgttga cgttgcataa acgggagaga gtatcaaaag
    42841 tatctacgat catctatatt tttatggggt ggctctgtgt cgtggctgct gttcctcttt
    42901 atcactctct tggaatgact ggtgttattt tgatggtagc aggcgggctt tcttacacga
    42961 taggcgcatt cttttatagc ttgaattcta tccgctatat gcatgttgtt tggcatttgt
    43021 ttgttatgtt aggtgcaggt tttatgtact tctctgtttt attctttact taataaaaaa
    43081 aataaagcta tggatgggac agctctaaat aacagctagt ctatccatag cttttttata
    43141 tcgtcaattc gcctgtcccg ctttttacag gattctcatt acgctgatac gtttcaaatt
    43201 gatgcggata acgatttttt tcatccattt ctccttgact agtattgatc agtgaccaat
    43261 cagaccagtc aacaggtgga aaataagcat ctccatcgaa tacatgatgg atgactgtac
    43321 ggtataatat cgtacaaaat ggcagaaatt cttgataaac agctgaccct ccagcgatga
    43381 aaaagatacc gtctgtttct ttttccagag caagcacgtc ttccacacta tgcatgacat
    43441 gcacgccttc tgctttatag gaagaatctc tcgtcaaaac aatcgtctga cgattcggca
    43501 acggacggct acccatccct tcgaatgttt ttcgtcccat cactagtgta tttgcttctg
    43561 tcatttgttt gaagaatttc agatcatttg gcaaacgcca aggaagacgg tcttcttttc
    43621 caatcaatcc gttttcatcc tgtgcccaaa ttgcagctaa cattttctca tcctttctga
    43681 ttctaaacag cgataggagc tttgatcgcc ggatgtgggt catatccttc taattggata
    43741 tcttccatct caaaatcaaa tactgactgc ttttcctgat tcaaaactaa agtcggaaat
    43801 gcgcgtattt ctcggctcaa ttgttctttc atttgttcga tatgattgat ataaagatga
    43861 gcatctccta atgtgtgaac aaattcgcca accgctaaac ctgtttcatg tgcaattagg
    43921 tgagtcagca acgcataact tgcaatgttg aacggaacgc ctagaaacac atccccactg
    43981 cgttgataca attggcagct tagccgtccc tcttgaacat aaaattgaaa catcgtatga
    44041 caaggaggca aagccatgct aggaacatct tccggattcc atgcagaaac gatcaatctt
    44101 ctagaatcag gagtcttttt gattgcttcg atcacttgtt tgatttgatc gataaagcca
    44161 ccgtcttttg tttcccagtg acgccattga tagccgtaga tgtgtccaag atcgccgtat
    44221 gtttcagcaa aggaatcatc ggttaggatc ttctcgcaaa aaagcttgct ctgcttttta
    44281 tattcttcat tgaattcagg atctgtcaaa catctatggc caaaatcagt catatctggt
    44341 ccattatagt ctggactttt gatgtagcgt tcaaatgccc attcatccca aatatggttt
    44401 ttattttgta atagatagcg gatattcgta tctcctttta aaaaccaaag caattcgctt
    44461 ttgatcaatc caaaaggtac tcgtttcgtt gttaacaaag gaaacccttt ttgcagatca
    44521 aaccgcattt ggtatccgaa taagcttaat gtgcctgtgc ctgttctgtc ttctttacga
    44581 tgcccttctt ccaacaattt tcttcctaag tcaagatacg cttgttccat cctttttcct
    44641 cctaatcttc tacccattca ctcaagtatt cccaccgtgc cattttttct tcaagttgtg
    44701 cttctgtttc agatacgtcg ttttgcaatt cttgtaatcg agtgaaatta tctccttgat
    44761 gattcatctc ttcttggagc aatgagattt tttcttcaag ttctgcaatc tcatcttcaa
    44821 tcgtttccca ttctttttgt tccatgtaag ataatttctt cttttcagca ggttccttgc
    44881 tctgtatttt tggtttcgct ttttcagcag gctgctgggt ctctttttgc ttcagtaaat
    44941 aactggacat gtttccgtaa taaagttctt gttttccgtt tccttggaat actaataact
    45001 tatctgctac cttgtccaag aaataacggt catgggaaac agtgatcaca gcacctggaa
    45061 aacttctaaa ataatcttcc aaaatcgtca atgttgctat gtccaaatca ttggtcggtt
    45121 catccaataa taaaacatta ggttgctgaa tcagcagttt caacaaaaat aaccggcgtt
    45181 tttctccgcc tgaaagtttg cctatcagtg tcccatgcat aaatcttgga aataaaaatc
    45241 gttcaagtaa ttctgctact ccgatttgac tgccatctgc tgtttttgct tcttcagcag
    45301 cttcttgtaa gtaagcaatc atccgtttat ctggtgccat ttcttcattt tcttgtgtat
    45361 aatacgcaag atgaaccgtt tcaccgatag acattgtacc gctatccagg ggaattctgc
    45421 cagctagaat attaagcaat gtcgattttc ccgcaccatt tttaccagta atacctagtc
    45481 tttcccgaga ttgaatcaat aaatctaaat gttctagcag tgtttggtca tttatacggt
    45541 aactgccatc tttgatttcc agtacttttt tccctaaacg ttgtgtcgct aggttcagct
    45601 ctaaatcatc ttcttggttt acttggtaca agttctcctt tagatcttcg aaacggttga
    45661 tccgtgcttg ctgcttggtg ccacgtgcct gtacgccagc cctcatccaa gctaattcct
    45721 gtttataaag ccgcttgcgc ttttcttctt gttctacttc tacacgatca cgttctgctt
    45781 tttctaagac gtaagcttca taatttcctt tatactcgta taattttcca aatgaaagtt
    45841 cgaagattcg gtttgctact ctatctagaa aataacgatc gtgggtaacc atcaatagtg
    45901 cgccacggta ttgtttcaaa tagttttcca accactcgat cgcttcataa tctaaatggt
    45961 ttgttggttc gtctagtaat agtaaatcgg gttcatcaat caacacttgc gccagactaa
    46021 ctcgtttttt ctgaccacct gataaagtac tgattttttt atctaattca ctgattccta
    46081 atttttgcaa aataatcttt gcgttggtat ctgtagtcca agcatcttct ttattcattt
    46141 gttcttctgc tttagcataa cgtttttgca catcagggtc ttctccgttt tcccctagtt
    46201 cgatcaatgc ttcctcatat gctcgaacag tttggatcaa ggggctgttt ccttgaaaga
    46261 ctgcttggag aacagttaat tcatcggaaa attcagctgc ttgagaaaga taaccaatac
    46321 ggtaatcagt tggataaaac actgtttgcc gatcaccgtc gctgctatcg ataccggcaa
    46381 taatatttaa gagacttgtt ttacccgtcc cgttcgtccc aatcaacccg atacggtctt
    46441 tttcgtggac caaaaaagaa atctggtcaa acaaatcttt ttcaccataa gtttttttga
    46501 ggtcagttac ttttaattct ttcaacatgt tccctcctta tcttggatat tgtatcataa
    46561 aaatagtgaa cttcaggaaa atttttttgg tatttgttct ttttgtgttc tcaaaaaaag
    46621 aacaggaccg attcgatcct gttctgatat gttttgtttt ttataaaact tgaaaatttt
    46681 taccttttta ttttattgga ctcgtttttt ttgactttgc tcgaaacgca aatcggaaag
    46741 aaaagttatc agatggtttg ttcatcgtat aagattctaa caaattgata ttcctgcgat
    46801 agctgttcgc agaaaacgtc gtgatttttt cagcaacttc gtattcatca atgatttttc
    46861 tttcaagata ataacctctt agcatttttt tcgtaaacag actatcttgc tggatcatcc
    46921 acgaagaaat caaattcccc gagttcattt ttattccttc aagtttgcgt tgctggataa
    46981 aaaattgaac catcgtttca tcataaatat cagtcaagtt atctaaactt tcaataatca
    47041 actgagtatt tttttgatat gtctctctga tttcttggat atcttcatca ctaataaatg
    47101 tctgacgatc agcatatctt ctttgccaaa acattttcgg atggagaacg attcgcatta
    47161 acctacggac aacaatgaac caaaagccga taaaagacaa aatctggctt gtgatagaat
    47221 gttcgaatcg agaaagaaaa cgggagtaaa aacgataacc attagaagaa agcttgcccg
    47281 aacgataact ttcatccaat ccatcccgtt cgatcgacaa aatcagtgct tgtatttctt
    47341 gtacttcttg cttttcatca tcggttaaat cttccaagta cagatcacgt atccgttctt
    47401 gataattttc tatcactgct tcagtggcta aaaattcttt tgtattacga tcaatttcct
    47461 ttatttcttt tcgcaaaact tcaacgacct cttctaaaat catcaaagca ttcaaatccg
    47521 tgcttttcgc ttcctctgtt tccgtcagca taggcaagac aatgatccct atgaccaaag
    47581 tcactaagat gacacccgcc gtcagaaaca aaagcaaaga ccgctggtaa aacatcatat
    47641 tattgattga aaatggcaaa ataaaaatcg tagccaaact gactgttccc tttacaccgc
    47701 caaatgttaa gatcaagatc tcgttcaact gttttttgaa ctttttactg ccatctttga
    47761 atacataaaa aagactgata gaaatgaatc tgatcacaaa aagcatgaca gagattaata
    47821 cgataatcgc taataaaaga ccgtttggat ataaaccgtc tccccatact ggcgagaaca
    47881 cttgcgtcaa ttcgattcct aaaaatataa atactaatgc attcagtgta aaagcaatag
    47941 ttgtccatgt actgtgggac aagctcgaca attctgcatc aaacacagag attttcctaa
    48001 aaccagaagc ttggagaata cctgctgcca ctgcagcaat gattccagat acatcaaatg
    48061 cctctgccaa tacataggct agaaaaggaa gcaaaagttc gatcagcaga taccctgtga
    48121 catcttgtgc tgatgctttt tcgatcaaat gaattatttt ttgtttgaac cagacaagca
    48181 aaaagccgat caaagctccc ccaatactag aaactactag actcatcccc gcgtttactg
    48241 ctgaaaaaga tccggtgatc agcgcaccaa gagcaaactg gaaggcagta acaccagatg
    48301 catcattcaa aagaccttct ccttccaaaa tatgcatcgc ctttttaggg atattcactc
    48361 gtccagaaag agaactgact gctaccgcat ctgtcggacc taacgctgct ccaaaagcaa
    48421 agcaggcagc aagtgggaca cttggcaaca ggaatgataa cgtcgcacct accccgacaa
    48481 gggtcaaaat gactcctcca aatgccaaga acaaaattgt cccgaaattt ttgagaatcg
    48541 aagaaatatc tgctttttct ccttctctaa ataacaatgg tgcaataatc ataactaaga
    48601 acaattcagg ttcaaaatcg attgattgtc cccactctgt cagacctaat accactccaa
    48661 ggaaaatttg gatcagtgga gctggaatca ttggcaaaat gcggctagct atgttcgata
    48721 aggtgatcgt tactgcaaat acaataacta actcagcaaa ttccatcgtc catccccttt
    48781 attttttatt ttgtgtaagc atttttaaaa attgttcttt tttctttaaa attttggttt
    48841 cgactacttc aacctgtgtt tggttcccct ctaattcaca ctgtacacgt tttgactcca
    48901 aacgaagcag atcatgtttc agacgttcca actgacgata catcaatata tttcttttac
    48961 gcaacttaca aagtatttga cggcggtcat ccatcgaaag ggcgccatag cgatcatgca
    49021 acagtttttt ttgttgttca aaactctctc tcattttcca gatctcctat acacaaactt
    49081 ccatcattat agcaaagttc tttttggatg aaaaatattt cattgtttca tcgtttattg
    49141 ctagttagaa atttccataa atttttcaac gcctcattct attccaaata gtatcagata
    49201 tcaagtaaaa cttaagaaag ataaaagttt ctgacaaaag gatgtttact ttcccattcc
    49261 tgcctttctt gttcttttat cacaacaaaa aaatggggct gcgacaaaaa tcttgtcaca
    49321 gtcccttatt ctgaataaac ggtgtggaag agacagccta tcagtagtaa taagacacat
    49381 ctctttcaca gcctcatttt tgcgtaaaaa tcgatttatt cgaaaaattc cccgtataca
    49441 tcttctacac gttgatctgg attgatcacg atatacaagt tacctgtttt ttcgctgatt
    49501 tttgttgatt gatattgatt gtctaatcct tctgttcctt tatctagata tgggaagtac
    49561 acaagatcag gtgtgccatt tcggtaaagg atttccattc gctcgatatc ttcgtatttc
    49621 aatgcgcgat taaacatgcc catttccagt ccgcctaggt tgatatcatg tgtcgcaact
    49681 gcgtcccctt catggaagat ttcgatttta aatccagcac acggatggat ttcaacaaat
    49741 tcactgccat tgattcgtcc aaaacttgtt gtgatttgtt tgatccaaag atcgccgata
    49801 tgacggcggt tgatcgtcca ggtttctcca tttctaaaat aaaattttag cgctgtcatt
    49861 tctttactca ttaaaaagac tcctttatga tgtaatttat tcttgtacta cctcagttta
    49921 gcacaaattt atgtaagtgg tcacatctta tgttttttct tctaatgacg tatctgccca
    49981 ctttaagtag ccaacaaagc tgagcagata cagtctagtg ttttttaaat caatttttct
    50041 ttcgataaaa aatccaaaac ctgtttttta tcatttttta ttctttgttc tacgacggct
    50101 ttttcagcta attgcaaagc ttcactaacc cacttaccag gtttttttcc agtttcttct
    50161 agcaagattt taccatctac tgctaattcg cttaatgact tgataggtaa acgatcatag
    50221 gattctccta cggcatccat ctgaggtgtt tcgtcaaaat agatcatcaa ttcttctact
    50281 tgcagtgcat gttttttgcc taagaagtac aattcgaatg gttccaaagc tccagtttct
    50341 cgttgtcgta gtccttggac aagtgcttga acgtctttaa tcatttggtt agaacacttc
    50401 cacgatttca aaaaggaacg aatctcattt tcttttagat ccagttggct gatcaataat
    50461 gcccatgctt ggctctcttc tttcaatggg acggatggta attcagcaaa acgtaaaagt
    50521 gcttcgcctt cttctttcaa accgggacag caaatgtagc actctgtttc aataaatgca
    50581 tggattcccg cggcccgttc atttcccaag agcatcttga tgaattccac atttatacgt
    50641 tctacagata ttttacctaa tagctcgtgg ttttctttga tggcgtagaa ggtttcttcc
    50701 tctaatttga accctagttg actgacaaaa cgcaacccgc gcatcattcg taaggcatct
    50761 tcatggaacc gctcatgagg attgcctaca gctcgcaaga tctgttcttc tagatcttgc
    50821 agaccatcga acaaatcgat gatttctcca tcttctttca aagcaaacgc attgatcgta
    50881 aaatctcttc ttttcaaatc ttctgccaac gaacgaacga attcgacatg atctggtcta
    50941 cggtagtcct gataagttga ttccgtccga aatgtggtga tctcatattg ttcatcattt
    51001 tctaatacca acacagttcc atgttcgatc ccaatatcta tcgtttttgg aaaaagctgt
    51061 ttgatttctt ctggaaatgc gcttgttgcg atatccacat catggattgg ctggttcagc
    51121 aagatatcac gaacacttcc acctacaaaa taagcttcat atcctgcatt ctctaatttt
    51181 ttcagaactg gaattgcctt ttggtattcc agaggtaatt gttttaattt catttttcca
    51241 acctttcctg cgtgtttctt gtcctgagat aaagactcag ttgcatggtg actgtttgac
    51301 aagtccgttt cagagtcttc aaggccagga caacttcatc ttgtttactc gtccttacgc
    51361 tcaaagcgat tatggtctct ttgattgaat ttttccatgt ttttttcaaa aacttcagtt
    51421 aagtcgatat tcaatgaatt agccatgatc atcgtaacga ataaaacatc accaagttct
    51481 tctgctaccg agttaactgg ttcagaagcc tttttgcttt tttctccata agtgtggttg
    51541 acttctcttg ccaattcacc tacttcttca gttaatcgag ccatctgagc taatggagag
    51601 aaatacccca ccttgaattg ctggatatat tcatccactt cctgctgcat actgtataaa
    51661 gaacgctttt tatccatctt tttccctcca aaactatctt accaaaactt taacgcaaat
    51721 cctagataag agcaattgag aaaatcgaat ttccatgcta catttaggaa ggaatttcta
    51781 aatggaaatt ctgtgaaaat acataattga aagctggaat tcatatggtc aaacgaaaaa
    51841 cagtaaaaga agttcttttt atcattatag gaacaagtat ttatgcattt ggtcttgttt
    51901 acttgaacat cgctaatcat ttagcagaag ggggagtctc agggatcacc ttgatcttaa
    51961 gagctttatt tggtattgat ccagcttata cgactttatt aatcaacatc ccattgattt
    52021 tactaggagg aaagatcctc ggtaaacgtt ccttggccta tactgttcta ggtacacttt
    52081 ctttgtcatt ctttttatgg atctggcaaa gggttccact agaaatcaat ttacagcatg
    52141 atctactgat cgtttctcta cttgcgggtt tagttgcagg tgtaggaagc gggatcgtct
    52201 accgcatggg tggaacaaca ggcggcagtg atattatcgc tcggatttta gaacaaaatt
    52261 acggcatcac tatggggcgt tccctattgg catttgatat catcgtctta cttgcttcat
    52321 taacctatat cgacttgaaa cgtatgatgt atacgttgat tgtttcgtat gttttcagtc
    52381 gtgtgattga ttcgatctta gatggcggat attctgcaaa aggtctactt gtcgtctcca
    52441 acaaaagcga agagattgca ccactactga tgaccggtct gcaaagaggt gtgactttcc
    52501 tttctggaga aggcgcattc tctggtaatt caaaaaaaat catctatatg gtcgttagct
    52561 ctagtgaatt aactgaagct aagaggatcg tccacgagat tgacgaaaaa gctttccttt
    52621 caatcatcaa tgtgcatgaa gtggaagggc aaggctttac ttatttgaag ccacagacaa
    52681 gattactgaa aaaactataa gaaaaaagac aaagataggc tatgaggaaa gattccacat
    52741 ccagctgacc tttgtctttt ttagtactat actattttta actaattgtt atgttcactt
    52801 acccggttac ttttcttttg tgtctgcaaa aggatctttc ggttaacttc atcagataat
    52861 ttcaaaaaag catccctatc tccatcttct agtgcttgat cgatttgagc ataaagcatt
    52921 tttagttttg cctcttcttc tttttcttga aaatatcgtt caattaattc agtatcttct
    52981 tcactgactg aagaatccca agaagaaaat ggattgtctt ctaagataga tagatattct
    53041 cttgtctgcc aagcattttc gaatatacat tccacatata aaggcttttt ccaattcaaa
    53101 cggatttcat gaaaaatctg gtctgtgtcc gtaaattctt ttccctgcaa aaacaatttc
    53161 atgggttccc cttcaacaga tagatcactg atctgcagtc ctcgatccgc aaaattcgcc
    53221 ccttcaacaa agtggacatt gcttaaaatc gcttcatgat tggcaagata attcaagatc
    53281 caaaaaactt ctcttcggct aaaagataca tgattgacca tccacgttaa aaaattcttc
    53341 ttttcccgta catcaattag catttttctc acctctctgt taggtcatct aataatgaca
    53401 gtgcctccat atctcctggt tcaagtgtta aataataagt caataattct tgcgctcttt
    53461 tcagctgccc ttcttctctt aagaaaaagg catactcttt caagaaatct ggttcatgtg
    53521 atagttcatg ataagcctgt tcatagtgta cagctgcaac ggtaaagtct tctaattcat
    53581 tataagcatg ggctaggttc cattctgcat aaggattagc agtttcttcc atctgattga
    53641 tcacttgtat cacatcttca tatctttcat catccaagta aagattactt aaagtaagta
    53701 atgtttcatc ttgtttgtct cctacttcta aagctttcag taaaagttct tctgctcgct
    53761 gtctatcatg cagacggaaa gcattttccg aggccaggtg ataaagttcg acttggtaag
    53821 gattttcttt gattccttct tctaacactg tttgtgcttc ttcaatcatt tcttcttctt
    53881 gaagagcttg tcctagatat agatataacg attgataatg cggattaact tctcgaagtt
    53941 cttccagata gttgattgcc tgctgatttt ctttcaattg aagattcgta aaagctagct
    54001 ggaacagtcg atcatctgtc ctttcttctt tcagtgcttc ttctaaaggt acgatagctg
    54061 cttcgaattc tccttgcata cttaaggatt gtcctaaacg ttcctgcatc gagattcctg
    54121 aaatttcatt tatcccgctt gccaacaatg tttcataaac cactgctgcc tctttgaaac
    54181 gatcaactga aaaatacaat tcagctagcg caaactggat caatggttca tcaggaagca
    54241 acccagctgc ttctttcaat tttgcttcac tcacttcagg gattccgata acttggtaca
    54301 aatcagctat cgccaataag ctttgcggat agtaatcact ttgcttatcc actttttcca
    54361 ggtattcaaa agctaagtcg atctcattat cttcgattgc aatttctgct aacgggatgt
    54421 tcagtccgtc attttctggg tattggttga tcagttgttc aaaaatctgt tttgcttcga
    54481 tcaaaaagcc aatagccagc aattcttcgc ctagttcagc taatatatct gctgggtctt
    54541 cttttaatgc tttttctaaa aagatttgtg cttgtgacaa gtcttcatct gataaagctt
    54601 gcaacatttt ttcgctattt gtattcattt caattccctc ttatctcact gacgatttgt
    54661 tgaaataccg tcattgttct caataattct ttttcgtttg gatgttggta tccgtctatt
    54721 atacctattt tttctaaaac aagtattttt ttgctttttt ctgccaaaaa aagaactttt
    54781 tccaaatact ccatctttga aatctgttcc agccagtgga ttggatagcc aagcaagtcc
    54841 agccacacaa aaaagttttt tagttggaaa tcaaaaggat atgcttggag attccacacg
    54901 aaatgaaaca tcattcctaa aaattttttc atactagcag aaagtagatg gccattttcg
    54961 gtcagataaa aagcttgttc aaacagttta ccataatttg acaggttttc tgcctctaat
    55021 tgataaaaat agatcatttc ttcgatcatc ccggcaaacg gccgagtcgt taattgcttt
    55081 ttatttggat agtttttata cagattttgc aaaaaacgat gatcacaaat cattccgcaa
    55141 cagataaaag tcaaaaagtc aatctgcttt ccgtccacct gccgctcgct gattgtttga
    55201 tcataaacga tgcgttctgg cagattgatg gtttgcagca ctacacgatt tggtagctgc
    55261 aaaatcgtcc gctgcggtgt taatgctcgg cttagagaac ggatcgatac agggatcatc
    55321 catagtgatg aatcaagggt tgtatgtttt tgaaaaaaac ctgccagatc catgatccct
    55381 tcatttccaa atccgatgat aaacatggat tgattttcgg gatagcgttt gacgaaatca
    55441 agcaggttct ttaattctgt taaatgattg cattgagtgt tggcacaaat ataccagtca
    55501 ttgtttgctt gatttgcaaa taacgggttg atcttatctg caaacaaatc ataatagcgc
    55561 tgattcgtta aaaataagat tggctgatca atggaaagtt ctcttagctg actgactagc
    55621 gtttctccgt aaaacacttt cgtcaacgca tcttctactt gataatcaag ttccatacgc
    55681 atccctcctc ttcttatttt tattttacca ttagtaagca aattctagaa aagataagag
    55741 caaagctcac ctcataactt gcaaataaat aaaaattaaa taaaatacat gtgttttcta
    55801 tgtatgacat acggattcgg aatctctgtc tcttatttat tccttttctg ctaggaaata
    55861 aaaagaaaag cacttgtagc cctagagcca caagcgcttt ttcaactgtc ttatttaaca
    55921 gcgtctttta atgctttacc tggtttgaat gcaggtactt tgctagctgg aatttcgatt
    55981 tcttcaccag tttgtgggtt acgacctttg cgagctgcgc gttcgcgaac ttcaaagtta
    56041 ccaaaaccga ttaattgaac tttttcgccg ttagctaaag catcttgaat agttgaaaat
    56101 acagcatcta ctgctgcagt tgcatctttt ttagttaaat cagtagctga agcaactttt
    56161 tcgattaatt ctgctttgtt tgccatggat agattcacct cctccacaag agatatgata
    56221 gaaatatcat ttagttaaaa ctttctgagt ggtccagaag tttaaaaaga aatattgatt
    56281 aacttcaata ttctgttatc acgatatcat atgaaccgct taatagcaag gtttttacca
    56341 attttcacat gcttttttta gtcatagaaa cgtaacaaac gaaactgtct ttttcttttg
    56401 aaaaaaaaac gtcgatatgg taaaatgcct attttctcct acgagggata attcggatgg
    56461 acgtcccttc aaaagtaaat gcttttctga tttgattttc taagaaacgt tcataagaaa
    56521 aatgcatcaa ttcctcttca ttgacaaaaa cgacaaatgt aggtggtttg acagcgactt
    56581 gtgtagcata aaaaatcttc aggcgttttc ctttatctgt tggtgttgga ttgattgcta
    56641 cagcatccat aatgatatca ttcaacacag ccgaagagat acgaagattc tggttcatac
    56701 ttacttgttc gatcatttca ggcagacgtt ccaaccgttg tttagtcact gcagaaacaa
    56761 aaacgattgg agcgtaatca agataccgga attcatcacg aatctcttgt tcgaaatcac
    56821 gcattgtatt ggtttctttc tttacaagat cccatttatt cacgacgata ataattccac
    56881 gaccagcttc atgggcgtaa cccgctactc gcttgtcttg ttcccgaatc ccttcttcag
    56941 cattcagtac catcaaaacg atatcagaac ggtcaatggc acgcattgca cgcatcacgc
    57001 tgtatttttc agtattttcg tatacttttc cgcgtttacg cataccagct gtatctatca
    57061 ttaagaattt ctgaccactt tcagaaacga agtgggtgtc gattgcgtct cgggtcgttc
    57121 cttcaatatc cgaaacaatt acacgttctt ctcctaaaat cgcattgatc aaagaggatt
    57181 taccaacatt tgggcgacca atcaaactaa acttaatcgt gtcgtcgtcc tcttcttcag
    57241 tctcttcaga gaaatacttc actgcttcat ctaaaatatc accaatccct agtccatggc
    57301 ttcctgaaac aggataagga tcgcctagcc ctaaggaata aaattcatag atatcgtttc
    57361 tcatttctgg attatctact ttattgactg ctaaaatcac tggtttatta cttctgtata
    57421 aaattttcgc aaccatctca tctgcgtctg tcacgccttc gcgtccgctg actacacaaa
    57481 tgattacatc tgcttcttca atcgcgattt ctgcttgatg cttgatctga tccataaaag
    57541 gttcatcgcc aagatcaatt ccgcctgtgt cgatcacgct gaattctcga cctaaccatt
    57601 caccttttgc ataaatacga tcacgagtta ctccaggagt atcttcgaca atcgagatac
    57661 gctcaccagc gatgcggtta aaaatcgtag acttaccgac gttcgggcgg ccaacgattg
    57721 caattgttgg atttgccata ttatttcctc ctcacaagtt tccaatctca cttagtttaa
    57781 cgaatgcatt gccaactttc aagcatagat tgtcaattaa acgaagtatt tttattcctt
    57841 attgaaatac taatcgcttt tcaaaagaaa aactgagaca aaagcagctg ctttcatctc
    57901 agtcacagat acaaaatcaa ttatttttca gaatcatcgg ttgctttttc ttctacttct
    57961 tctttcaacg catcacctaa aatgtctccc atagtaaatc cagtattttc ttctggtaat
    58021 tcataagact caacattttt tggttcttct tctgaagctg gtttttcttc taaagctttg
    58081 atactcaatg caatacgatg ttcttctgga tggatctcta atactttcac tttcacttcg
    58141 tcaccttcat gaagcacttc atgtggtgtc gcaatatgtt tatgtgaaat ttgagagata
    58201 tggactagcc cttctactcc agggaacact tcaacaaagg caccgaagct tgtcaaacgt
    58261 ttaactgttc cgtctaatac tgtgccaact ggagcttttg tatcgatttc tgaccaaggt
    58321 ccaggctgtg tgtctttgat tgacaaggaa acacgttctt gttcaggatt gatagacaac
    58381 actttgacat ttacatcatc attgacagcc aatacatcac ttggttttgc tacatgacta
    58441 tgtgaaatct ctgagacatg gactagaccg tcaattccgc ctaggtcgac aaatgcgcca
    58501 aaatcagtaa gacgtgcaac acgtccactg agaacatctc cctcatggat agttgaaaga
    58561 atttcttttt tcttttcttc tctttcgttt tccactaccg ctttatgcga taagatcaaa
    58621 cgattttctg atggttcgat ttcgatgatt ttaaagctca atgttttgcc tttatagtct
    58681 gaaaagtctg caacaaaatg atcttccacc attgatgcag ggacaaaacc acgtacaccg
    58741 acgtcaacga ctaaaccgcc tttgacaaca tttgtaacag gtgcttcgat gaccttgccc
    58801 tcttggaaat ctctttcgat atcttcccaa acttttttag catctaaacg gcgttttgaa
    58861 agtagatagc tgccgttttc tttatctttg ccgatcgtac tgataacgac taaatctaat
    58921 acatcaccga cttttactac ttcgctgata tcttctaccg gcaaagttga taattctttt
    58981 gccggaacga ctccttcaac tccagcccct tcgattccga caatcacttg tttgtcttca
    59041 atgactaaaa cttcgccctt aacaacatcg ccaactttta cttcttgtac gcttttcatt
    59101 gcgtcttcca ttgactggtt gttctcttga ttttgatcaa attctgtcat aacacttgtc
    59161 ctcctaacca acattctgcc gtaagtacgg tactgttttt agtttaattg attattcaaa
    59221 aaaaataaag cgatttcgtt cgttttcctt gaaaaaacaa ttgtttttca ttttttttct
    59281 taatttaaaa aagaagataa ccttttttca aaacaacagc ttttattgct gcaacgactt
    59341 ctggaatcga tttgccagtc gtatcgatac gaacagcatc ctctgcttgc ttcagcggag
    59401 agacgtccct tgttgaatca taatggtctc tttcctgtat ctctttcgtc agtgtttcaa
    59461 gatcagtcgg aattcctttt tcttggttct ctttgaaacg tctttctgca cgctcactta
    59521 cgcttgctac taaaaagatt ttcacttctg cattcggtaa tacagctgtc ccgatgtctc
    59581 ttccgtccat gacaacgcca ccgcgttccc ctatttttcg ttggacagca actaattctt
    59641 ctctgacttt tccatgtgca gagacttcag aaacagcttt tgtgacatct ggttgacgga
    59701 tttctaaagt cacatctttt gatgctatga atacatgctg tccatcttct tgttgcgaaa
    59761 aagagatagg atactttttg atcagcgaaa ctagcccttc ttcatctgaa aaagatacat
    59821 tgttcgtaat agctaaataa gttactgcgc gatacatggc accggtatct gtataaatat
    59881 aattcaattc ttttgctaat atcttggcaa cggtactttt cccagatgaa gcaggcccgt
    59941 cgattgcaat actgatttgt gtcatgatat aactcctttt tctcagaaaa aaagccgcaa
    60001 agcgcggcta ttattattta attcttaatt cttgaccagg gtaaagcata aagttgtttg
    60061 gatctaagcc attcaattca aacaattgat cagtggtgat ccctgcacgc tcagccactt
    60121 gttttggacc ttcgcctgct tgaacagtcg ttgttgaacc ggctgcagct gatgaagaag
    60181 gtgttgtttc tgctgcattg ctgcttgaat cccctgcttg ataagttgcc tcacttgagg
    60241 aagtcggtgt tgtctctgct gcaatactgc tttctgacgt tacacctgta tcttgagaca
    60301 cagtagattc ttccgcgatt gtactagatt ctactgttga agtctcagtt gactcctctt
    60361 ttgttgtact tgtagaactt tctactactg atgtcgaagt ttcagaagaa gttgctgctg
    60421 ttgcattgtt gcttgatcca tttctgatcc agaaataagc acctgctgga atagcgatac
    60481 ataagaccag taaaatcaaa aatagcgaaa gaaataatgt atttcctttc ttttgttgtc
    60541 tctgagaact tcttgatgaa acttcatcat ctgtatcgta gattggttgt tcccatggtt
    60601 cttgtgtttc gttattttct gtagattgat gtctatcttt tctgctcact gttttacctc
    60661 ctaaactcta tcgtgtgtat tataccatag gaaaaagtgt cttgtcctaa aaaatcaatg
    60721 ttccatgttt tttttaaata aatttagtaa aatcgttccc caatgaagtt gttcagtctt
    60781 agaaaaaagc ttcctctttg gcagaagttc tgcttttgct ccatcaatat cacaacaatt
    60841 ctgcggcttt tcggagagtt tttcgccaaa ataagctaag atgaattttc tgcggcagtt
    60901 ttcttcatta atatagcgaa gcatctgctg caggcggaat tctttttctt tttcctgacg
    60961 tttgagccgt tccacccaat tttctggttt ttcagactgc tggatcagct ccatccattt
    61021 tttttgcagc tcatcaaagg gagcttgctc tgtggcatat tccaaataaa gctcaaaact
    61081 ttgtcgctgt tctctagaaa gttgattaaa gaaataatgg atacgttcat ccccactttg
    61141 ataaaaaaga attgctgcac tttcttcctg atctctgcct gctcgaccga tttcttgtac
    61201 atagttttct agactgtctg gcaaatcata atggaccaca taacggatat cagatttatc
    61261 gatccccata ccaaatgcat tcgtagcaat gagaaactgc agctgatttt ttacaaactg
    61321 ctgttgaagt tgtcttcttt gagctgcatc aagaccaccg tgatagtatc caactgtaaa
    61381 acgttccctg aataaatgat acaatcgttc gacttctttt tttgttgcac aataaatgat
    61441 tgccgctcct tgtgctcttt ccatgaagtg ttcaagttct tgttcttttt gctgcgtttt
    61501 ccgaacaaaa agcgagatat tttgccgatt gactgattgt ctgatctctt ttggtggtcg
    61561 atcaaatagt acatgacgta tatctcttgc tactaaatct gttgctgtcg ccgttagtgc
    61621 cagtgtgacc gggtttccta aaagctttcg gatttttcct aactgttgat attctggacg
    61681 gaaatcaact ccccattgag acacgcaatg agcctcatct actacatata acgcaatatt
    61741 ttgttttttc agatgctcta aaagagaagg attagtcaac atttcgggac tcaaaaacaa
    61801 aaatttatat tgcgatagat gacggaggac gtatcttctt tcagattgcg aaagtaaact
    61861 attgaaagca gctactcgtt tctcccctcg tttttgtaat tgcgtcactt gatcttccat
    61921 tagagaaagc agcggtgaca cgatgatgac cagaccttcc attaaatacc ccgtcaattg
    61981 atagcaaagg ctttttcccg ttcctgtagg caaaatagcg agagtatctt ctccatcaag
    62041 caacgcctga ataatttctt cttggccggg acgaaatgaa tcaaatccaa aatatttttt
    62101 taattcattt attaacattc tgtatcgctt cctttctcaa tagatagatt tgatacaaac
    62161 gaaattctcc ataatctaat tgtccatcta cattccattc gctaaagcgc cactctcgca
    62221 cggaatcctt atttggttcc aaacgttcga ttgtttctgc agagagaatc cactcgtatg
    62281 gaaaatcatc aaaaaacaag gcccactcaa taagatgatc tgttactgtt cctcttttta
    62341 aatgacggat tgccatgatc tggtcaattg tctgaccatt cataaacatc tgcttggtga
    62401 tcatcatgct ttgattgaaa ttttgctgta ataatgaatc gatcaaagcg tgccaaagcg
    62461 aatctggccg tttttcgatt tgtgctaaca aaaggtggat acatctgctt tgttgcagat
    62521 aaaccgctga ctcatcattg ttttttgcca gctgataagg aagcagccct gttcgctcat
    62581 ttccagataa ttggttagct aaaaaatctg ctgcttcttc tggtatctcc gaaaacagct
    62641 gcaccaaact ctcatacgca gtatcaatca aaatttcacg tggcaattgg ctttcactaa
    62701 gccatttttt tagttggaaa gtataaaaag gacttgtctc tgctggaaga tagtcttgaa
    62761 tacccgttgc cagattagaa attacttgaa cagcaaattt aatgagccgc caactagttt
    62821 cccctgttct gccatagcgg tcaaaacgca aaccagtatg ttctatttga tgttcggaca
    62881 acatatcagc ccctgcaggc gttagctttg cttcattttc atttatttcg atccaacctt
    62941 gctgcgcaat tttttgcaaa atttgataaa acttatcctg cttcaattct ggaaaactgt
    63001 tatggataaa caaaagttcg tgggtaaatc cataaataag tactgaagtc gttcgttttc
    63061 ccacaaggag ttggtaaaga gaactaatcc gcagcttgtg tcctgatgaa aatagcgata
    63121 aaataaaaac aatcatatta ttctccttaa gttaggatgg ttagaaatgt cactactatg
    63181 taaacttgta cctgaacgct gtattgcatg cggtctttgt caaatcgaag cgccagatat
    63241 tttcgattat gatgaagaag gaatcgtttt atttgcccaa gaaccagagg ctcatcaaca
    63301 gttcgtgatg ccggaagaag aaacatatgt aaaaaaagcg tatcaaaaat gtccagttcg
    63361 agcgatttta ttagaaaata aaatataaat ataaacgaaa ctttaaaaac gtacactaag
    63421 ttctctaaac ttgaaaataa gccgggatct gtttctcttg ttccgtgaaa tcccgactta
    63481 ttttgaaaga ttcatttgct atctatagtt aattgactag ttttttcacg gaccctatag
    63541 gacccattta tattatttca actttttcag ccagttttcc aatgtattga cgatcacagt
    63601 ttgctggtct gcgttagaaa tttctgcctc tccatctcct ttttgctcac catagctgcc
    63661 aaaacctcca tgatttccgc cattgattga tacatactct gttctttccg gtaaatactg
    63721 cttagcttct tgataagctt cctgattcaa tactttatct tttgtcgccg tgatcgataa
    63781 aacaggaaca gctgattctg tcaatgttcc ttttttgtcg ggataacttg ccatgaagaa
    63841 tacccctttt aaatgtttgt ctgaatgatt cttattgaca taacgacttg ccattacgcc
    63901 acctaatgaa tggccaccga tcacataatc ggaatttgga aaatcttctt tgaccttatc
    63961 cgctttgttt ccgcccatca ctgctagatc aagtggcatt ttgacaatag cgactgaaaa
    64021 gccgtttttt gctaaatctt gcgcccaaat actataactt tctggctcta cgagagcacc
    64081 aggataaaaa ataagtaatg gagactttcc atgattcttt ttctctcctt caaaaaataa
    64141 gtaatcttct ttgttttttc cttcttttac caacacttgc gcttctgacg atggttcgta
    64201 cgtattttgc tgtatatagt aataaccggc tccaccgagt aatcctaaaa tgatgactaa
    64261 gccaattagt attcgtctaa atattttcac tttctcccac cttttctatc tgagtgaccc
    64321 ctttttctta atcaagtatg attatacctt aaaaaaataa gagagaaatg caaactttcc
    64381 agcatcaaat aaaaaaagac ggaatatcca tttgaatatc cgcccttttt gtatgtttat
    64441 ttttatttag cacataaacg atacacagct tctgcataaa tttccattgc tttatacata
    64501 ctacttaatt cccatcgttc attcgcctgg tgcataaagt caggagtatc agggaacatc
    64561 gcaccgaatg ccacacactg gttcatcgta cgagcaaaag tagcgccgcc tgatacaaac
    64621 ggttcagtca tgtctccagt aatatcgcga tatgtcccca atagattttt cactaactca
    64681 ctgtctagtg gcacgtataa tgaatcaagg aagtcaaatt cttcataagt caacccatat
    64741 ttttctgctg ttttagttaa tttagctact aaatcatctt tcttgaatgt aacagggatt
    64801 ctcatatcta caccgatttt tgtttgttct ggtgtgattt ccaaacttgc aaagttcatc
    64861 gttagctcgc cggattgttc atcttctgtt ttgcccacga cagcttcacc agttgcattt
    64921 tcttgaacta attttcctaa gaaattgatt gggccaaaat caaatacttc tgaaagagca
    64981 attgccagac gagaaatagc attcactcct tgtgctgcat ctttcgcgtg gatactttta
    65041 ccaagaacga cgatgccttc accttgttct tcgaaatcga attcgtgttt tttcaatgct
    65101 tcttttactt cagaaagctt ttctccagag taaggagctg catcaggaac aacattcaac
    65161 gctcctccag cttttacaga aaactcgttc gttcctggtc ctgtcaagta agcttgcaat
    65221 aagccttttt ctgcataaat taaaggaaat tcagcatctg gcgcaaatcc ttgcgtaatt
    65281 ccttcttctt tttcgttata tttttctaaa caacgccata aattttcttc atctgtacca
    65341 aagataaaac gaatacgtgt cttgaattcc acgccagcat caatcaaagc tttgacggca
    65401 tacattgccg caatagaagg acctttgtca tcttgggatc cgcgaccaac taaccagccg
    65461 tcttttatag ttggatcgaa tggttttgtt tcccaattat tttcgtcccc tgctggaaca
    65521 acgtccatat gacacaagat accgaataat tcatctccag agccaatctc tgaataaccg
    65581 taatagcctt ctggatcttt aaatgttcta aatcctagct tttcactgat ttccaatact
    65641 ttatccaaag cttcaatcac ttttttaccg aagggatgtc cttgtcctga atctgcttca
    65701 tctagtacgg aaggaattcg gatcaattcg ctcaatgatt ctaatgcttg ttcatgatgt
    65761 tgttctgtca caaatttttt catgatatta tcctcctaat atgttaaaaa gggtcatgtc
    65821 aaattcctcc gacacaacct cttcttctca aataatgacg ttttaagcct gctccatcag
    65881 caagcagaat gttcttaaca aatgatgaaa gaaaaaacac tcactgaatg gagctattga
    65941 cttatccacg tgcttatcga tagccgttta tcggtttctt cattcttatg atagaccttg
    66001 tacctgtatt tgtctaggtt tagaacattg ctgctacacc taggatcaca cagatggcta
    66061 caaataatat cgcaattaat ttaccggtaa atttccacca cactgaaaca tcgaaacgag
    66121 caattgctac agcacccatt acgatagcag atgttggtgt aattaagttt actaaaccag
    66181 atgcagattg ataagcagtg attactaaat ccttaccgac gccggcaaat tcacccatag
    66241 gtcccatgat tcccattgtt gcagcagcta agccggaagt tgaaggaatc aagaatgaca
    66301 tagggatata gaaaataaat gtaataatga tgaatacact tgaagataat gaacttaatc
    66361 cttgttcgcc ccaatgtaag atggtgtctg tgattaatcc attgttcata accacttgga
    66421 taccacgagc aacagctact acgattgcta caccgatcaa atcagaagca ccagctaaaa
    66481 attcagagac aaattcagac tctttcatgc cataagtgat tgctactacg atggacatta
    66541 ctaagaataa catagtaatt tctgggaagt accagtcacc taatggaagc atgcttttac
    66601 ctagaaccgt acctagtacc ggaaggtttg ttaaccagtt tgtcaatgat tcaaaaatcg
    66661 tgaaattcgc attcaaggtt gtccaaggaa tcaaactgat gatcatgata ccaaaagtaa
    66721 taaagaacaa tacattaaca tgtttttgtt ttttcgtgat tacttcttga cgaccgattg
    66781 cttcaatatc gaaatgcttc atatcttctt tacgttggtt gtatactaat gattttgttg
    66841 gatctttttc gatctttgat gcataacgat acacatatac aatagaaata gctaacaaaa
    66901 taacgaacat caagaatcga agaccaatac cttcacccat cgaaatccca acagcttgtg
    66961 aagctacgcc tgttgcaaaa ggatttaccg ttgaagctag acagcctact tgagacccta
    67021 ctaatacgat tgctacagct actactgtat caaagcctac agccatcatg actgggatca
    67081 gtaaagcata gaatggaatc gtttcttctg ccattccgta agtagagccg cctaatgcaa
    67141 ataacagcat caatactgga ataagcattt tttcttttcc tttgaatttc ttcacaactg
    67201 aagcaatccc ggcatctagt gcccctgttt tgttgataat acctaaaaat ccaccaacaa
    67261 ccaaaatgaa gaaagagatt gggattgcag caggtgtttc tttagttagc tcccccgctg
    67321 gtacacctag catcccattg attggcgcca tgaaaatatc ccacagccct tgaggatttt
    67381 gttgtactcg ttcatatgat ccagcgacga tgttgcctgc atcgtctaca ctatagtgcc
    67441 cagctggtat aaaccaagta agtacagcga ttactgcaat aataataaat aagaccgtat
    67501 acgctgaagg catttgaaat tttttctttt ttgtttgttc catcttcttc ttcctttctt
    67561 ttccgttaga gacaacaatt ttggctctaa tgttatttta tcaaaaagag tatcagaaca
    67621 aaaataataa cacgcatttt gtaaaacttt tgttcaaaaa gttcgttttt ccctattccc
    67681 tcctcttttt tcaggttttt aaagctttct tctttttcat tgtagcgttt cctttttttt
    67741 ataaacaaac attttcgtct tttaaaagga gaagaatttc acatttgcaa agaattgatt
    67801 gatttttatg cataaatcaa aaaaatactc aaaacaaaca aataaaacgt ttacattatt
    67861 gcatgtcttg tatttttttc caaaaaaaaa gaccaagaaa tttcttgatc tttttgatat
    67921 ttatttgact gctgtatgac gttgttcgaa ctgatgttgt tttcgactta accatggaag
    67981 aagttgcgca tgcagcacta aaaagacagc actcacgatg aatcctttga tcaagttgaa
    68041 aggaacgatc ccaatcagta catagtttgc taaagacatg cccagcatct gtgttgcgtt
    68101 cagccctaaa aacattaagt aaagaggcgt aataacgaaa taatttgcga tactcataaa
    68161 aatagttaga gcgatggttc ctgtaatgac acctaaaaat ttgtttcttc ctttatgctg
    68221 tttctttaca ctaaagaaat aataaatcgg gaatgtaaag atcgttgttg ataagaaact
    68281 agcagtgtct cctaccaaat tatccggaga aaaacctgta gtcagcagat gtaatactga
    68341 acgcaagaac gctgttgcaa ctccggccgc tggaccgaat aagaacatgc tgatcaatac
    68401 cgagatatca ctaaaatcga tcttcaaaaa actaaaagcc ggcatgatcg gaaaagcaaa
    68461 atactgtaaa actaatgcga tcgcagcaaa catcgctact gcaaccattt tctgtacacg
    68521 ggtgttcttc atttgaaatc ctcctgttag gactcctctt cagcgaactt tttgacgttt
    68581 ctctccaaat gaaaaaactc tattttccag ggaaaataga gttagagtta tttgatcaag
    68641 cgtcgtcaaa taagctctat cttctttatc cagactatac tgtcggtatc ggaatttcac
    68701 cgattcagct atttttttgc aaaataggtc gtggactata accaccggtc gggaatttca
    68761 ccctgcccct gaagacgaac cttatataat ttttgtttac atattcatta tacatatcta
    68821 ttcaaaaaac gcaacatcta acacttataa aaagtaagtg ttcattttga gtggttcagc
    68881 aaactgctga cttctttttt gttcagttca cggtaatccc caggacgtag gccttgtaaa
    68941 gtcagatctc cgtatttttc acgttttagt ttttgcacag gaagaccaac tgcttgaagc
    69001 atatttttca cttgatgatt acgtccttcg tggatcgtca attccacgat actgtggttt
    69061 gtcttaggat ccactgataa aatttggaat ttagctggag atgtttttcg tccttcgatc
    69121 tttattcctt ttgtcaaagg tgcaagcatc gattttgtag cgatcccttt tacctttgca
    69181 acgtaaactt tatccacttc atgttttgga tgtgtcaggc gctgagcaaa atctccatca
    69241 tttgtcagta gcaagatccc cgaagtatcg taatccagtc gacctacagg atagatacgt
    69301 tcttttacca tcggcaaaag atccgtaaca acttttcttc ctttgtcatc tgcaacagca
    69361 gaaataactc ctttaggttt gtacaataaa tagtaaccgt attcttcttg ataaattggg
    69421 acttcatcca cttccacagt gtcatgtttc ccaactttta tccctaattc ccttacaact
    69481 tgtccgttta cttttacacg tcctgcaagg atgaattctt ctgcttttcg acgagaagtg
    69541 atgccagcgt gggcaattac tttctgcaat ctctccatta caattattct cctttattca
    69601 ttccatcaaa gaatagatct ggtgacacgt cttcttcaag tgaaatttcc atttcttgtg
    69661 tatcaggcag ttcttctaat gatttcaaac caaaataatc taagaagtag ccagttgttc
    69721 catataatat cggccgacct ggtccttcta ctctaccttt ttcttcaatc agtttatggg
    69781 cagctagacg ctgtaccgct cctgaaacct gaactcctcg aatctgctcg atttcagctc
    69841 ttgtgattgg ctgtttataa gcgatgattg ctaaggtttc gacggcggct tgagataatg
    69901 tgtttgatgt cccttgcgca tatcgcttaa gtaagggagc cagctctttt ttagtcgtta
    69961 atacataatg gctttccgtt tcaaaaatat gcaaagctga gtgcgagttt tgagtgtaag
    70021 attcttttaa ttctttcact aaacttgtga cttctgcttc gctgagattt aacagtccgc
    70081 ttaattcttc attactgatc ccttcctcgc caacaataaa aagaagggct tcaagttcac
    70141 ttaatttcgt catgttcggt atctcctact gctggataaa gcataattga ttcatagttt
    70201 tctttttgtt gcgcaatgat ctgccctttt ttcatcaatt ctagcaaagc cataaaagta
    70261 gtgacaactt cacttttaga aggtgactca aaaaaagaat cgaaagtaca gccatgcgca
    70321 tttcttttta tacgctcttc gataaatacc attcgttctt cgatcgttgt gtcatcgggc
    70381 gtgatcgttt tttccacttt ttgagaaaat ttcttacgtt ctaagatttt ctgcatggca
    70441 cggatcaaat cgattttttt gacttgtcct cgcttcaacg gcttttcagc atccgctaac
    70501 ccatcgatgt tcataggttc ttttgtgtaa taaagacttc gttcctctgc tttttctgtc
    70561 agtctttcag ctgcatattt atatttacgg tattctaaaa gctgagtgac caattcttct
    70621 cgaggatctt cttcccataa ttcttcatcg tctgtgacga attcttggac aggaagcaat
    70681 gtcttgcttt tgattgccat caatgtagca gccatcacta agtattctcc tgcaacagct
    70741 agctcgagtg ttttcattgt atggatataa ttcatatatt gctccgtgat cgtagcgatc
    70801 ggtatatcat atatatcgat ttccatttgc tgaatcagat gaagcagcag atcgagagga
    70861 ccctcgaata catctaattt aatattgatc tccgtcaact cattcactct ttcttctttt
    70921 tctttgctag ccattttcca atgtaaggct ctgtgatttc agttgcacgg atggagacat
    70981 ccgggtactt ttcctgtaat tcatctaaca tttgaaagcc catattctca ccgcgataag
    71041 acggattcaa agaaatgtca tggatgatca gctgatcgtt cgttgcccag taaataccaa
    71101 taatcccttg aatgttggtt gaaccttctg gcgtaaagaa aaaaagttca taatcgtctt
    71161 cagattcgta cgtatcaatc tccttcaaca aagaagtttt acctgtcagt ttcttatgga
    71221 aactcaatag tcccatagca attttacggt tttctttccg gtaatgtgtt aacatcttct
    71281 caccttccta tgactaagcc cttggaaaat gctgcttata aacatccgtc attcttttct
    71341 tcgtgatatg tgtataaatc tgagtcgtag agatatccgc gtgacctaac aattcctgta
    71401 ctgtccgcaa atcagctcca ttttctagca gatgtgtggc aaagctatgc cgcagcgtat
    71461 gtggtgttac ttctttgtaa atcccagcct ctcgtactaa ctgcttcaga tttttccaga
    71521 ttccttgccg agaaagacct ttcccatgat tattgacaaa tacatgcatt tcagatgcgt
    71581 cctttacaag taatggacgt gcttcgtcta agtagcgttc cagccattgg atcgcataat
    71641 cacctaaagg aatgatccgc tctttatctc cttttccaac tgtctgtacc agtccaagag
    71701 aaagatgaag atctcccaat ttcaatccaa tcaattcact gacgcgcatc ccagtagcat
    71761 acatgacttc caagattgca cgatctctta tccctaacgt ttttgtcgta tcaggtgctt
    71821 caatcagctt ttctacttct gtcagagtca atgtacttgg taatttctgt gctttcttcg
    71881 gcgtatcgat atgttgcatc ggatcatgat cagtgatacg ctcttgccgt aaaaactgat
    71941 ggaatcttct gagacttgag atcatccgag taacagttgc tgtcgctttt ttttcttcat
    72001 gcaacttctc cagaaaacgg atgactaaaa agcgatcgac atcctgccat gaagcaattt
    72061 tctgctcctg tagaaaatgc aagtactgtt ccaaatcacg ttcatagctt tgtcgtgtat
    72121 tggcggacaa tccccgttca atattcaagt agtggagata atcgttgatt tgctctttca
    72181 taataatccc cctcatgcct ctctatcata tcagataccg cttcattctg atagtctttt
    72241 tccataatct ga
//
LOCUS       DBNICI010000002        63235 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.002, whole genome shotgun sequence.
ACCESSION   DBNICI010000002 DBNICI010000000
VERSION     DBNICI010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 63235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.002"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            135..662
                     /locus_tag="AC1ZCC_000073"
     CDS             135..662
                     /locus_tag="AC1ZCC_000073"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIQ1628365.1"
                     /translation="MKTIPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIED
                     KEMSPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKA
                     DTKTYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNT
                     FVELKKKRRRTQKFL"
     gene            790..1473
                     /locus_tag="AC1ZCC_000074"
     CDS             790..1473
                     /locus_tag="AC1ZCC_000074"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIQ1628366.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1782..1855
                     /locus_tag="AC1ZCC_000075"
     tRNA            1782..1855
                     /locus_tag="AC1ZCC_000075"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1816..1818,aa:Arg,seq:tct)
     gene            2014..2373
                     /locus_tag="AC1ZCC_000076"
     CDS             2014..2373
                     /locus_tag="AC1ZCC_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HIQ1628367.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2496..4127
                     /locus_tag="AC1ZCC_000077"
     CDS             2496..4127
                     /locus_tag="AC1ZCC_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIQ1628368.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4189..4830)
                     /locus_tag="AC1ZCC_000078"
     CDS             complement(4189..4830)
                     /locus_tag="AC1ZCC_000078"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIQ1628369.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    5016..5092
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5112..5420
                     /gene="rplU"
                     /locus_tag="AC1ZCC_000079"
     CDS             5112..5420
                     /gene="rplU"
                     /locus_tag="AC1ZCC_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIQ1628370.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5447..5791
                     /locus_tag="AC1ZCC_000080"
     CDS             5447..5791
                     /locus_tag="AC1ZCC_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HIQ1628371.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5804..6097
                     /gene="rpmA"
                     /locus_tag="AC1ZCC_000081"
     CDS             5804..6097
                     /gene="rpmA"
                     /locus_tag="AC1ZCC_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIQ1628372.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6531..9338
                     /locus_tag="AC1ZCC_000082"
     CDS             6531..9338
                     /locus_tag="AC1ZCC_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIQ1628373.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9529..10020
                     /locus_tag="AC1ZCC_000083"
     CDS             9529..10020
                     /locus_tag="AC1ZCC_000083"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HIQ1628374.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10123..11112
                     /locus_tag="AC1ZCC_000084"
     CDS             10123..11112
                     /locus_tag="AC1ZCC_000084"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HIQ1628375.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSIGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11145..11948
                     /locus_tag="AC1ZCC_000085"
     CDS             11145..11948
                     /locus_tag="AC1ZCC_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HIQ1628376.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11969..12883
                     /locus_tag="AC1ZCC_000086"
     CDS             11969..12883
                     /locus_tag="AC1ZCC_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HIQ1628377.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVERWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12966..13340
                     /locus_tag="AC1ZCC_000087"
     CDS             12966..13340
                     /locus_tag="AC1ZCC_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HIQ1628378.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13479..13724
                     /locus_tag="AC1ZCC_000088"
     CDS             13479..13724
                     /locus_tag="AC1ZCC_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIQ1628379.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13896..14960
                     /locus_tag="AC1ZCC_000089"
     CDS             13896..14960
                     /locus_tag="AC1ZCC_000089"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIQ1628380.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15206..15631
                     /locus_tag="AC1ZCC_000090"
     CDS             15206..15631
                     /locus_tag="AC1ZCC_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIQ1628381.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15624..16073
                     /gene="nusB"
                     /locus_tag="AC1ZCC_000091"
     CDS             15624..16073
                     /gene="nusB"
                     /locus_tag="AC1ZCC_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIQ1628382.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16170..17024
                     /locus_tag="AC1ZCC_000092"
     CDS             16170..17024
                     /locus_tag="AC1ZCC_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HIQ1628383.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            17025..18362
                     /gene="xseA"
                     /locus_tag="AC1ZCC_000093"
     CDS             17025..18362
                     /gene="xseA"
                     /locus_tag="AC1ZCC_000093"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIQ1628384.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18367..18591
                     /locus_tag="AC1ZCC_000094"
     CDS             18367..18591
                     /locus_tag="AC1ZCC_000094"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIQ1628385.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18593..19483
                     /locus_tag="AC1ZCC_000095"
     CDS             18593..19483
                     /locus_tag="AC1ZCC_000095"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIQ1628386.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19492..20310
                     /locus_tag="AC1ZCC_000096"
     CDS             19492..20310
                     /locus_tag="AC1ZCC_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HIQ1628387.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDGLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20417..20866
                     /locus_tag="AC1ZCC_000097"
     CDS             20417..20866
                     /locus_tag="AC1ZCC_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HIQ1628388.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20884..22566
                     /gene="recN"
                     /locus_tag="AC1ZCC_000098"
     CDS             20884..22566
                     /gene="recN"
                     /locus_tag="AC1ZCC_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIQ1628389.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22621..22737)
                     /locus_tag="AC1ZCC_000099"
     CDS             complement(22621..22737)
                     /locus_tag="AC1ZCC_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HIQ1628390.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22810..23766)
                     /locus_tag="AC1ZCC_000100"
     CDS             complement(22810..23766)
                     /locus_tag="AC1ZCC_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HIQ1628391.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23937..24305
                     /locus_tag="AC1ZCC_000101"
     CDS             23937..24305
                     /locus_tag="AC1ZCC_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HIQ1628392.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24475..24906
                     /gene="mraZ"
                     /locus_tag="AC1ZCC_000102"
     CDS             24475..24906
                     /gene="mraZ"
                     /locus_tag="AC1ZCC_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIQ1628393.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24927..25886
                     /gene="rsmH"
                     /locus_tag="AC1ZCC_000103"
     CDS             24927..25886
                     /gene="rsmH"
                     /locus_tag="AC1ZCC_000103"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIQ1628394.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25891..26292
                     /gene="ftsL"
                     /locus_tag="AC1ZCC_000104"
     CDS             25891..26292
                     /gene="ftsL"
                     /locus_tag="AC1ZCC_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HIQ1628395.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26293..28485
                     /locus_tag="AC1ZCC_000105"
     CDS             26293..28485
                     /locus_tag="AC1ZCC_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIQ1628396.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28511..29473
                     /gene="mraY"
                     /locus_tag="AC1ZCC_000106"
     CDS             28511..29473
                     /gene="mraY"
                     /locus_tag="AC1ZCC_000106"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIQ1628397.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29487..30848
                     /gene="murD"
                     /locus_tag="AC1ZCC_000107"
     CDS             29487..30848
                     /gene="murD"
                     /locus_tag="AC1ZCC_000107"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIQ1628398.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30874..31962
                     /gene="murG"
                     /locus_tag="AC1ZCC_000108"
     CDS             30874..31962
                     /gene="murG"
                     /locus_tag="AC1ZCC_000108"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HIQ1628399.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFSENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31977..33197
                     /locus_tag="AC1ZCC_000109"
     CDS             31977..33197
                     /locus_tag="AC1ZCC_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIQ1628400.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33353..34678
                     /gene="ftsA"
                     /locus_tag="AC1ZCC_000110"
     CDS             33353..34678
                     /gene="ftsA"
                     /locus_tag="AC1ZCC_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIQ1628401.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34701..35942
                     /gene="ftsZ"
                     /locus_tag="AC1ZCC_000111"
     CDS             34701..35942
                     /gene="ftsZ"
                     /locus_tag="AC1ZCC_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIQ1628402.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35959..36636
                     /locus_tag="AC1ZCC_000112"
     CDS             35959..36636
                     /locus_tag="AC1ZCC_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIQ1628403.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36650..37249
                     /locus_tag="AC1ZCC_000113"
     CDS             36650..37249
                     /locus_tag="AC1ZCC_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HIQ1628404.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37273..37551
                     /locus_tag="AC1ZCC_000114"
     CDS             37273..37551
                     /locus_tag="AC1ZCC_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIQ1628405.1"
                     /translation="MALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLAKIC
                     EPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37584..38381
                     /locus_tag="AC1ZCC_000115"
     CDS             37584..38381
                     /locus_tag="AC1ZCC_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HIQ1628406.1"
                     /translation="MGGENFDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFL
                     DPRQSYILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYP
                     SKFASLSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVA
                     VRLEPIKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNW
                     TENTRPDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38402..39109
                     /locus_tag="AC1ZCC_000116"
     CDS             38402..39109
                     /locus_tag="AC1ZCC_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HIQ1628407.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39371..42169
                     /gene="ileS"
                     /locus_tag="AC1ZCC_000117"
     CDS             39371..42169
                     /gene="ileS"
                     /locus_tag="AC1ZCC_000117"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIQ1628408.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMCMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42218..43744)
                     /gene="zwf"
                     /locus_tag="AC1ZCC_000118"
     CDS             complement(42218..43744)
                     /gene="zwf"
                     /locus_tag="AC1ZCC_000118"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HIQ1628409.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43759..44406)
                     /locus_tag="AC1ZCC_000119"
     CDS             complement(43759..44406)
                     /locus_tag="AC1ZCC_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIQ1628410.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44590..44931)
                     /locus_tag="AC1ZCC_000120"
     CDS             complement(44590..44931)
                     /locus_tag="AC1ZCC_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HIQ1628411.1"
                     /translation="MIKKMIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFS
                     DSQQKQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDES
                     ADTSYKLLSEK"
     gene            45096..45824
                     /gene="sfsA"
                     /locus_tag="AC1ZCC_000121"
     CDS             45096..45824
                     /gene="sfsA"
                     /locus_tag="AC1ZCC_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HIQ1628412.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45840..46853
                     /locus_tag="AC1ZCC_000122"
     CDS             45840..46853
                     /locus_tag="AC1ZCC_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIQ1628413.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46853..48130
                     /locus_tag="AC1ZCC_000123"
     CDS             46853..48130
                     /locus_tag="AC1ZCC_000123"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIQ1628414.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48193..50895
                     /locus_tag="AC1ZCC_000124"
     CDS             48193..50895
                     /locus_tag="AC1ZCC_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIQ1628415.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAVIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            51047..51364
                     /locus_tag="AC1ZCC_000125"
     CDS             51047..51364
                     /locus_tag="AC1ZCC_000125"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIQ1628416.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51394..51714
                     /locus_tag="AC1ZCC_000126"
     CDS             51394..51714
                     /locus_tag="AC1ZCC_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HIQ1628417.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51822..53282
                     /locus_tag="AC1ZCC_000127"
     CDS             51822..53282
                     /locus_tag="AC1ZCC_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIQ1628418.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53350..53571
                     /locus_tag="AC1ZCC_000128"
     CDS             53350..53571
                     /locus_tag="AC1ZCC_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628419.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53602..53784
                     /locus_tag="AC1ZCC_000129"
     CDS             53602..53784
                     /locus_tag="AC1ZCC_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HIQ1628420.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53784..54197
                     /locus_tag="AC1ZCC_000130"
     CDS             53784..54197
                     /locus_tag="AC1ZCC_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HIQ1628421.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54320..55501
                     /locus_tag="AC1ZCC_000131"
     CDS             54320..55501
                     /locus_tag="AC1ZCC_000131"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1628422.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55572..55736)
                     /locus_tag="AC1ZCC_000132"
     CDS             complement(55572..55736)
                     /locus_tag="AC1ZCC_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628423.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55890..55963
                     /locus_tag="AC1ZCC_000133"
     tRNA            55890..55963
                     /locus_tag="AC1ZCC_000133"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55924..55926,aa:Arg,seq:cct)
     gene            complement(56032..57171)
                     /locus_tag="AC1ZCC_000134"
     CDS             complement(56032..57171)
                     /locus_tag="AC1ZCC_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIQ1628424.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(57285..57869)
                     /locus_tag="AC1ZCC_000135"
     CDS             complement(57285..57869)
                     /locus_tag="AC1ZCC_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HIQ1628425.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(57952..58374)
                     /locus_tag="AC1ZCC_000136"
     CDS             complement(57952..58374)
                     /locus_tag="AC1ZCC_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HIQ1628426.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFETEADKIAIMILLNIFIENELTDE
                     SQFKLENFMEFYAINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            complement(58392..58712)
                     /locus_tag="AC1ZCC_000137"
     CDS             complement(58392..58712)
                     /locus_tag="AC1ZCC_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1628427.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            59012..59152
                     /locus_tag="AC1ZCC_000138"
     CDS             59012..59152
                     /locus_tag="AC1ZCC_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628428.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            59450..59707
                     /locus_tag="AC1ZCC_000139"
     CDS             59450..59707
                     /locus_tag="AC1ZCC_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HIQ1628429.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(59678..59986)
                     /locus_tag="AC1ZCC_000140"
     CDS             complement(59678..59986)
                     /locus_tag="AC1ZCC_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628430.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            60064..60810
                     /locus_tag="AC1ZCC_000141"
     CDS             60064..60810
                     /locus_tag="AC1ZCC_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HIQ1628431.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            60825..61028
                     /locus_tag="AC1ZCC_000142"
     CDS             60825..61028
                     /locus_tag="AC1ZCC_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1628432.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            61044..61382
                     /locus_tag="AC1ZCC_000143"
     CDS             61044..61382
                     /locus_tag="AC1ZCC_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HIQ1628433.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            61369..61539
                     /locus_tag="AC1ZCC_000144"
     CDS             61369..61539
                     /locus_tag="AC1ZCC_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628434.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYKTKKPVGRD"
     gene            complement(61637..62302)
                     /locus_tag="AC1ZCC_000145"
     CDS             complement(61637..62302)
                     /locus_tag="AC1ZCC_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628435.1"
                     /translation="MSLIDFIKQFDWNNIVKTIKDFILPITSLVFSTFAFYISYTDRK
                     NKKFNLKLDFFAECEEWLIDRESDSKPDVYHQNKFRIIDSVLLTNNSSLPVTIIEFSI
                     SGIPDHLNAFTMIGDNYSVTVKSMYDELPHGIRAYSGKSLKKGADLSKFPPLSLPITI
                     PPYESKITTLVFRYDESLVEKNITINVLTSRGTAKFNRFVSSSQISQLDTGYAPPQLD
                     EFD"
     gene            62357..63055
                     /locus_tag="AC1ZCC_000146"
     CDS             62357..63055
                     /locus_tag="AC1ZCC_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HIQ1628436.1"
                     /translation="MQELVILKNKEAVTTSLQVADSFEKEHKNVLRDIEKLKEDVLNF
                     EQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNIKVMEVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDENE"
BASE COUNT        21031 a        10618 c        13304 g        18282 t
ORIGIN      
        1 aataaaaaat gagataaaga tgaggaaata gaattagaaa catataatta ttaaagggaa
       61 acatttttac agctataatt tgaaaaaaat ttatcgttat tatatgatta agaaggattg
      121 aataaagaaa gaagttgaaa acgatacctg attacaaaaa aataactttt ataagttcta
      181 taacgttgat tatattttta attcttattc ttctcgtcgt cttggttccc cgaatttttg
      241 gtttgactcc ttatattatt gaggacaaag aaatgagtcc atattatgca caaggaaatc
      301 tgatatatgt acgaccgaaa aatacaaatg agattttagt cggagatatt attacatatt
      361 atgaaaattc aggaaaaaaa atctcgacgc gtcgcgtaat agccgttgaa aacaaccacg
      421 aagattttta tacaaaagct gatacaaaga cttatattga gcctggagta gtaaaaaaga
      481 gaaatattat cggtagtccc atctttcaga ttccatatat tggactagtc ctttcaaaaa
      541 cggcatttgc atggattcag gggttattct ttacgattgc tttttgccta acaggaatca
      601 cagcatggaa tacctttgta gagttgaaaa agaagcggag acgaacacag aaatttttgt
      661 aagaatgtta aaaacataaa tgtattccag agatccaacg actcttctga acatgaaaca
      721 cgagaaaaca ctcatgcagc ttgtagattt agtcagttgt tcgtataatg tgtgaagagg
      781 aggagtagta tgcgcaatga aatgatgcat cgtcctgtag tcaaagaaga actattggat
      841 tttatgcgta cccagcaaaa aaaattaccg ggtgagctag gcaaactgga agaagaagct
      901 catgtagcag aagtaccagt tattccccat gaaacagttg tatttttgaa atttttactt
      961 ggacagcttc agcctgaacg aatcttggag atcggagcag ctatcggttt ttcatcaagt
     1021 gtcatggcca cgacaatcgg agaaaacgct catgttacga caattgatcg tttcgatgtc
     1081 atgatcgaga aagctaaaaa aaactatgaa cgtctaggac tgacagataa agtgactttg
     1141 cttgaaggtc aagcagcaga tatcttgccg gagttgtctg gtccgtatga ctttattttt
     1201 atggatagtg caaaatcaaa atatattgaa tttttacctg aatgcttacg tttgttaaga
     1261 aaaggtggcg ttttgatggt agatgacatc ttccaaggag gaacgatctt gctcccagat
     1321 gaagagattc cccgtggcaa acgagctatc catcgtaaat taaatgaatt tcttcgtgtt
     1381 gtgatggatc atcccgattt gacatcgaca atcttgccat taggtgatgg tgtgatctta
     1441 atgacaaagg aatcagaaac aatcgagttg tgatacatga ttatggcagc ctgtagataa
     1501 tcgattgaat cgagggctca attcgaactt gatgtaaaag tttctatatt tgtaagtcaa
     1561 ttaaaggata caggaaatct aaataaacag gagtagcatg aagtcaatgt agctcctgtt
     1621 ttcaattgat aaccttagaa cagctgaagg agaatgtctc gttttaaaag attgatttag
     1681 tagttgggcc aaaataattc attatttgct aattaaataa ctatttcgta ttgacagaag
     1741 caatttaatt atctataatt attgatgttc cgctgattct tgtcatagta gctcagctgg
     1801 atagagcatc cgccttctaa gcggacggtc gggggttcga atccctcctg tgacgctaaa
     1861 aaaccgttag aaactgaggt ttctaacggt ttttctttgt tttttgtgcc aatattgttt
     1921 gttttcaaag gcaaataaaa taaaaaaaca tccttatcga gtacaattat aaggagcaaa
     1981 aaaataaaac tcatcagaaa agaggaagaa gatatgacga tactatctag aattttagtg
     2041 actctagttg caattgaatt tttttatatc atgtatatcg aaacggtgag aactgattct
     2101 gacacgacaa gtagggtttt taaaatgagt aaagaagagt taagcagaaa atctgttcaa
     2161 acattgttta agaaccaagg agtgtacaat ggactattag gagtgggact actttatgga
     2221 gcttatcttt cttccgcatc taaagaaatc acaagtatgc tattaatatc tatttttttt
     2281 gttgcattat atggcagttt gactagcgat cgttggattt tactcaaaca aggtgggcta
     2341 gctctgttgg cattgctttc tattttctta taaatataga aaaaataaga atttatctaa
     2401 caggaaactt tgtgaaaaat attactttat ctcatgaaat atgatattct aatgaatggt
     2461 attttagaaa atttcatgaa aaaggagtta gataggtgtt caaaaattat gtttcctcac
     2521 taaaaaaaga attttcaggt tatgatcttt caagatttca gaaagatctt ttggctggga
     2581 tcacagtagc tgctgtagca ttgcctttag ccttggcttt tggggtatca agcggagctg
     2641 atgcggccgc aggtttgatt acggctatca ttgccggtat cgttattggc ggtttatcag
     2701 gcggttttta tcaaatatca ggtccgacag gtgcgatggc agctatcttg atgtctattg
     2761 cggcaacaaa gggaatgcaa ggtgtattgc ttgctacttt tttagcgggt ctatttttac
     2821 ttgcagcagg acttttccga ttaggaacac tgacttcttt tatcccagca cccgtaatta
     2881 cggggtttac ttcaggtatt gctatcatta ttgcattagg tcagatcgat aatttatttg
     2941 gtgttcactc ggaaggtaca aacgttattg aaaaactaag tagttatcaa acgataggat
     3001 ttccgataaa cacagcctct ttgatcatgg gagggctagt cattttaggg atgtttatct
     3061 atccaaaaaa atgggcgaaa cgaatgccta gttcgctgct ggctatcatt ttggcaacag
     3121 cagttatgct attgttccat ttaccagtag caactgtggg taagattcca caaacattga
     3181 tcagtagtaa tcgtttgaat atgggagatt ttagcttttc tgccttgcaa caagtggcag
     3241 ttccagctat tagcattgct ttattaggaa tgattgaaag cttgctatgt ggtgcttcgg
     3301 ctggccgaat ggcaaataag cccttagata gcaatcaaga attaatggcg caaggaattg
     3361 gaaatctgtt gcttcctttc tttggcggga ttcctgctac tgcggccatc gcccgtacaa
     3421 gcgtggccat caaatctggt gcacaaacta gattagctgg tatgatacat gcagttgtct
     3481 tactgctctc catgcttgtt tttgcgccag tgatgtcaca gattccaatg ccagcacttg
     3541 caggtgtttt gattgtgacg gcttggcgta tgaatgaatg ggaaacgatc aaggagctat
     3601 ttactaataa atattggtca gcagttctat tgtttatcct tacgatgatt tgtacagtta
     3661 tctttgattt aagtattgct atcgtgatcg gggtgatcgg cggctgtgta ttttttgttg
     3721 tcaaaagcgc tgctatcacg atttcattag aaacgatcga ttggcaaaga atggggctgc
     3781 cagaaacgga aaagctgaat aactggacag tagtgtatat tagtggtcca ctgtttttta
     3841 tgtcttcaga aaaactcaaa aataaattgg aagaactaaa tcatcacgat ggtgtcattt
     3901 tctccatgcg aggcgtacca agtattgatt tgacggcacg tgcagttttt gatgaatttc
     3961 aagaaaaagc agagcaaaga ggacaaacga ttatctatac ttccttgcag ccagaagtag
     4021 aaaaacaact ttctcacttg tgggaacata cattcacgca tcaacatcaa acagttgctc
     4081 atgcgttgac agctttacat gcgaaatata ctgcaattag ccattaaaac aatgaaaaag
     4141 aacagacaag actgtaaact gaagatttta tggtcttgtc tgttcttttt aatattgctg
     4201 taaaagtgaa gctagtactt gccagtcttg atggataaca ggtacaaggg agcgattgta
     4261 gaaaatagct gctggatgaa aaagtggaag aattcgatag tttttttctg accattcgta
     4321 ggcatccttt tgttctgtta attgtaagac aggccctgta tagagttgac catgattttt
     4381 ggtaaccgta tattctttgc caagtaaacg ttgaagacca atgtttccaa gtgtcgcaat
     4441 taattttggc tggaccttca tcagttcgta atccaatatt ggtgcatgcg caaatacttc
     4501 ggaacgagta ggtgtccgat ttggataaac agtttctgac tgttgtgtcc gtttattgat
     4561 ccgatgagtc acgcgatacg ggcggctcct tacagcactg gtgatataca cctcttctcg
     4621 tgttaagcca gttgaagaaa gtgcctgcat caattctttt ccagcttgac cgctaaaagg
     4681 aacatgcgta tcaatctctg ttttccctgg ggcttctccc acgagcatca gtttaggatg
     4741 aagaggtcct tgcccagcga taaatccttc tacaggacgg ccgttcattc ttaaagaagc
     4801 ttcttgtatc agttcttttg ggtattccat ttatgctcct cctttttctt ttattataag
     4861 aataaaagaa acacaatgcg actattttat ttggtagaac tgtgaggata gaaaagcaga
     4921 agtattgctt gaatttttgt tttacttttt gattttaaat ttagttgact aacggttgct
     4981 gttctggtaa gctagtactg ttgtaattgt ggactccaca gctacaaccg ctcagaacaa
     5041 gtaataagca cagcatatgc tgtcacttcg gatggcgagt ctaagtatat cagaaggagg
     5101 tgctttatag catgtacgca attattaaaa ctggtggtaa acaagtaaaa gttgaagtgg
     5161 gacaagcaat ctatgttgaa aaacttgacg ttgaagcagg agaaaaagtt gtttttgacg
     5221 aagttatctt agtaggcgga gaatctacaa aagtaggcgc tccaactgtt gcaggtgcaa
     5281 ctgttgaagg tactgttgaa aaacacggca aacaaaagaa agtcgttact ttcaaataca
     5341 aacctaaaaa acattcacac cgtaaacaag gtcatcgtca accatataca aaagtggtta
     5401 tcgacgcgat caacgcttaa tagcgtttga gaaagaaggc ctatatatga tcaaaggaac
     5461 atttaagcga aatgacgcag gtcagatcgt ttcattcact ttgaccgggc atgctgatgc
     5521 tggtccttat ggaagtgata tcgtttgtgc gggtgtttct gcgttagcga tcagcactgt
     5581 caacgggatt gcctcattag ccggttttga gccgattgtt gaaatgaatg aagaagaagg
     5641 cggttatctt tataccgaag tcacttcagg aatgacacaa gaacaaaaca atattgctga
     5701 gattctatta gaaaatcttt tattaggatt acaatcgata gaagctgaaa actcagaata
     5761 tatccaaatt aaaacaatta atgataaata ggaggtgcag accatgttgt tatctatgaa
     5821 tctacaatta tttgcccaca aaaaaggtgg cggttctaca tctaacggcc gtgactcaga
     5881 atcaaaacgt ctaggtgcta aacgtgctga tggacaaaca gttacaggtg gatcaattct
     5941 ttaccgtcaa cgcggtacaa aaatctatcc aggtgtaaac gtgggtatcg gtggagacga
     6001 cactttattt gcaaaagttg atggcgtagt acgtttcgaa cgtaaaggcc gtaacaaaaa
     6061 acaagtgtct gtttatccag tagctcaaga agcttaattg aatcttatga ttaaagaggt
     6121 tgtgacaaga cttttgtcac agcctttttt gtttataaaa ataaaagttg tagtggaggc
     6181 tacgaagttt actttgtctt tttacactta atattttttt gccaatttgg aatcaggatg
     6241 ttttgataac tatcgttttc aattgatgga agctatattg tttgctatca gtgaaataat
     6301 tatatggtaa acagtgttct ttttcttgtt tccattataa aaatcgtatc gggatttttc
     6361 gttcttttaa attgaacatt tctatctatc cgcgtgttat caacaaaaca aaatcaggta
     6421 aatagttgac gaaacagaaa taaatacata gtattatgtt ttgaagccta agtacaatag
     6481 tctatagaaa aggagcgata ccattggcaa aacgcattga ccgaatttat atgtatgtca
     6541 aagaaaaaac cgctcattta acaaaggcag aatatgatca aggagtaaca acacaggaaa
     6601 ttgctgaagt gttagggatt cagcgaacaa atagcagtaa agatctgaat cagctagtgc
     6661 gcgaaggaaa actgataaag acagacggac gaccagtacg ttacatatat caaacactcg
     6721 ttacgcacgt aaagccagtt acaaaacatg tagtcagtta caaagaaacg ccggtctctg
     6781 aagaaaaaaa tacactccaa atagatacta aggatatctt tgcaaaaatc attggagcta
     6841 acggcagcat gaaaaattca gtggaacagg caaaagctgc gattttgtat ccgccaagag
     6901 gattaaattg tttgattaca ggacctaccg gatcaggaaa gacttatttt gcacacgcga
     6961 tgtatcattt tgcaaaagcg catcatgtaa tagatgagga aacggaactg attgttttca
     7021 attgtgctga ttatgccaat aaccctgagc ttttgatgag tcatttattt gggtacgcaa
     7081 aaggtgcttt tacaggcgca gatgaagaaa aaatggggat tatcgatcaa gcggatggcg
     7141 gtatgctgtt tttggatgag attcaccggt tgccgcctga aggtcaagaa atgatctttt
     7201 actttatgga tcatggaact tacagtcgtc ttggggaaac aacgaaatcc catgaagcaa
     7261 atgtacggat cgtaggtgcg accacagaag acccaggttc ttctctatta gaaacttttg
     7321 ttcggcgtat cccaatcaat atcaaacttc cggcttttga aaaacgtcct gcaaatgaga
     7381 aagtcgattt agtaaaaatc atgattgccc acgaagcaaa ccgcacgcag cgaaagattt
     7441 ctttgacaga agatgtagca aaagcattga tcggcagtgt gacctatgga aatataggac
     7501 aattgaagtc aaatatccag ttagtatgtg cacgaggatt tctaaatcat atgcaatcac
     7561 ccgaaatctc gatcacaatc gatgatttga cagaagggat tcgtagcgga ttgattcaat
     7621 tagcaagcaa ccgcagtgca atggcagagc tttctaaaat attggagcca aagataactg
     7681 tagcaccaaa tgatacgatc atgaaaatcc aatcggactc ctatgagtta ccttataatc
     7741 tatatgatat catcggagat aaagcagctt tactgaagtc ggatggtttg gatcaagaag
     7801 caatcaatca tttcatttct acagatatca acatccatct gaagtctttc tataaagacc
     7861 atggtttttc ttttaatgca gacagcaaat tagcagaatt tgtagatccg aaaatcatag
     7921 aagtgaccaa tcagatttat gtaatggtca aaagatcttt accatatgag tttcagcaaa
     7981 actttattta tgctatgagc ttgcatatca gttctttttt gaaaaaaatc aacttaggag
     8041 aagaacggca tacgaatgat aacatccgtg agatggcttt tgattttcca gatgaatatg
     8101 ctgttgcgaa agaagtgcgt agttatatcg aaacgtattt tcaagtggag attccagaaa
     8161 gtgaagacta ttacttgacc gttctactcg tctcgttacg ggcaaatcaa gcaagcggca
     8221 gaatcggtgt agtcgttgca gctcacggaa acagcacagc aagcagtatg gtccaagttg
     8281 tccaacagtt gttagatgca gagaacgtac gagcagtaga catgcctttg gatatggatc
     8341 cgaaaacagc attggaccga attgaacgca atgtacaaga agtagatgaa ggaagcgggg
     8401 tcatactctt agtagatatg gggtcccttg cgtcgtttaa ttcgcaaatt caaagagaca
     8461 ctggaattcc agtacgtact gttgatatgg taacaacttc tctagttttg gaaactgtac
     8521 gaaaggtctc agtattaggg acagatctag atatgttgca tgaatcattg aaaaattttc
     8581 gaggttacgc agaaatatct tctgtagaat ctgttcagga agcaaaacct attcataaga
     8641 aaaaagctgt tttggctatc tgcgcatctg gcgaaggtac agcacaaaga atcaaagaat
     8701 tgatcgaacg agcggtaaac aagcgtcaag aaacagaatt agaagttcta gctttgtcga
     8761 ttgtggaagt gaaagaagaa ctgccagtta tccaaaaaga atatcagatc atagctacta
     8821 ctgggattac tgatttgaat atccaagccc catttattcc tttagaacgt tttattgatc
     8881 aaaatatcga agtcattttg gatcagttgc tgatggaatc agaattggag gaaactgaat
     8941 tcatctccct agatgaagag tcagctaaaa atacgtgtgt tgagtttatc tctgataact
     9001 tcatctttat taatggtagt aagttaatcg atccaatgtg gcaattttct acacaaatct
     9061 ctcagacgac gggaatcggc gatgaagaat atggattcaa aatcaaccta gtgatgcaca
     9121 cagcaggaat gatcgaacgt attattcgga atgaaccatt gactgtggaa gaaaacgaat
     9181 tgacaaatac aacgaatgat cctctttata gccaattggc agcctcagtt gttctgttgg
     9241 aagatcagat aaaagtaaaa gtgccgatag aagaaatgta ttatctgcta cgtctagtcc
     9301 ataatcagtt agataaaaaa gaatacactg tgccatgaat acacaaaaac gatacacttt
     9361 tatagtgtat cgtttttgtg tattggttaa gaatccttta aattcaatta gtgtatcgta
     9421 aaaaagttgg cacgcttttt gcttatagta gtgtgtgcta ggaggtggtt gttcacacag
     9481 aaatgatagg ggtttctgtg aagtagtgag gaagaaggag ttttgcagat ggatattcga
     9541 ttggcacgta tagatgatcg tttaatccac gggcaagtcg caacagtttg gtcgaagcaa
     9601 acaggtgttg aacgtatcat tgtgatcagc gatgtagtcg ctcaagataa attgagaaag
     9661 tttctactaa aagaagctgc accgccgggg atcacagcaa atgtaataac cgtgcagaaa
     9721 atgattcaaa tctatcatag tggattatta tcacgagcaa aagtgatgct tttattcacc
     9781 aatccccaag atgttgaagc ggttgtccgc tgcggtgtac agttgaagtc gttgaatatc
     9841 ggcggtatga gttttactga aggcaaatca atgattacca attttgtttc agtaaatcaa
     9901 caagatattg attcctttcg ttacttgaac agtcaagaag tggagttaga gattcgaaaa
     9961 gtacctgctg acaggaaaat ccttttgatg gaactactag cgaaaatcaa aaaggtgtag
    10021 caagaatggt tgttggaaaa ggtcaaataa ttctgttgta atggttagca attattaata
    10081 tttttgaatt tgttacaaca aaagtaaata ggaggtataa atatggtagg gattatccta
    10141 gcaagtcatg gacaatttgc tgaaggaatc ttacaatccg gttcaatgat ctttggcgaa
    10201 caagaaaacg tcaaagccgt gattttgaaa ccaagtgaag gtccagatga cttgagagcc
    10261 aaattggaag aagcggttgc ttcttttgat aatcaagacg aagtgttatt cttagttgat
    10321 ttatggggcg gaacaccgtt caatcaatca aacacattat tcgaagagca caaagataaa
    10381 tgggcaatcg tcagcggttt gaatttacca atgttgattg aagcctacgc atcacgcttt
    10441 tcaatggaat ctgctcatga aattgcagca cacatcattg aaacagcaaa agacggtgta
    10501 aaagtaaaac cggaagaatt agaaccggca gaagcaccga aagcagcagt tgaagacgca
    10561 caacctaaag gagcacttcc agaaggaact gtggtcggag acggaaaaat caaatatgta
    10621 ttagcacgtg ttgactcacg tctgttacat ggacaagttg cgacagcttg gacaaaagca
    10681 gtgcaaccaa accgtattat cgttgtttca gatgcagtat caaaggatga tctgcgtaag
    10741 agactaatcg aacaagctgc tcctccaggt gtaaaagcaa atgttattcc tattagcaaa
    10801 atgatcgaag tagcaaaaga ccctcgtttt ggaaatacca aagcattgct tttatttgaa
    10861 aatcctgaag acgtattgac agctgttgaa ggtggcgtag acatcaaaga attaaatgtt
    10921 gggtcaatgg cgcattcgat cggtaaagtc gttgtaagta aagtattgtc tatgggacaa
    10981 gaagacgttg aagcgtttga gaagcttgaa caaaaaggtg tgaaattcga tgtacgtaaa
    11041 gtacctaatg attcacgcga taacatggat gatattttga aaaaagcaaa agctgaatta
    11101 gcaaaagcgt aataaataat attaaaattt ataggaggct tatcatgtct attatttcaa
    11161 taattttagt cgtattagtt gcctttctag ctggtatgga aggtattctt gatgaatttc
    11221 aatttcatca accactagtg gcatgtacat tgatcggttt agttaccggt aacctcgaag
    11281 ctggtatcgt acttggcggt acgcttcaga tgatcgcttt aggttgggca aatatcggtg
    11341 ccgctgtagc accagatgct gcattagcat cagtagcatc agcaattatt ttagttttag
    11401 gtggacaagg tgttaaaggt gttccttctg cgatcgcgat tgccgttcct ttggctgttg
    11461 cagggttgtt cttaacaatg atcgtacgta caatcgctgt tccaatcgtt catttgatgg
    11521 atgcggctgc tgaagaagga aacatccgta aggttgaaat gtggcacatc attgctgtat
    11581 gtttacaagg ggttcgtatc gcggtcccag cagcagcgtt gctatttatc ccagcacaaa
    11641 ctgtgcaatc attccttgaa tcaatgccag catggttaac agatggtatg gctatcggtg
    11701 ggggaatggt cgtagctgta ggttacgctt tggtaatcaa catgatggct acaaaagaag
    11761 tatggccatt cttcgttatt ggtttcgttg tagctgcaat ttctcaatta acattgattg
    11821 ctctaggtgc tttaggtgtt gcattagctc ttatttactt gaacctttct aaaatgggtg
    11881 gttcatcaaa taatggaggc ggaagcaact ctggtgaccc tttaggcgac attttgaatg
    11941 actattaatc ttgaaggagg aaaataaaat ggcagaagaa aaaatcaaat tatcaaaaaa
    12001 agatcgttta gctgttgcgt ggcgctctac cttcattcaa ggttcatgga actacgaacg
    12061 tatgcaaaac ggtggctggg cattttctat gattccagca atcaaaaaat tatacaaaac
    12121 aaaagaagaa cgttctgcag cattaaaacg tcacttggaa ttctttaata cacacccata
    12181 cattgcttct ccaattttag gggttacatt ggctctggaa gaagaacgtg cgaatggcgc
    12241 gcctgttgat gatgtagcga ttcaaggggt taaagtaggt atgatgggtc ctttagctgg
    12301 tgttggtgac ccagtgttct ggtttacagt acgtccaatg ctaggtgcac taggtgcttc
    12361 attagctatg ggaggaaata tccttggacc aatcatcttc ttcttaggtt ggaacattat
    12421 tcgttgggca ttcatgtggt atacacaaga atttggctac aaagctggtt ctaaaattac
    12481 tgatgacctt tctggcggat tgttacaaga cgtaacaaaa ggtgcttcaa ttttagggat
    12541 gttcgtatta gctgcccttg tagaaagatg ggtatcaatc aaattcttac cagttgtatc
    12601 aactgtaaaa ttagataaag gtgcttacat cgaatgggat aaattgcctt caggtggtga
    12661 aggaatcaag agtgcatttg aacaagttaa taatggctta gccctttctc ctgaaaaggt
    12721 aacaacatta caagacaact tagaccaatt gattcctggt ttagctgcat taggattaac
    12781 attgttatgt atgtggttgc ttaagaaaaa ggtttctcca atcgttatca tccttggttt
    12841 attcgtagtc ggtgttgttg gacacgtcat cggactttta taatccataa aaatatagct
    12901 cgggcgttgt cccgagcttt ttttatatag tatactgttc ataaacaaat atctggaggg
    12961 agaaaatggt tcaatcaatc aatacaaaag tagacttggt aattgatgct acttcgttta
    13021 cgggtctgac agattacggg caaatcatga taggtgataa tggttttgag ttttaccatg
    13081 cgcgagatag acgcaaatat atccaaattc catgggaaga agtggattat gtactagcat
    13141 cagtcatgct aaagggaaaa tggattcctc gatatgccat tcaaacgaaa aaaaatggta
    13201 catacacatt ttcttccaaa gaacctaaaa aagtgcttcg tgcgatcaga gaatatgtag
    13261 atgaagatag aatggtacaa tcattgagtt tctttgatgt agtgaaacgt ggtattcgta
    13321 gtatgtttaa acgcaagtaa aaaataaaga aaacttctaa gaggaggttt tctttatttt
    13381 tttactttgc agaagaaaat atgaaatagc aaaaccaatt atttgtcccc tttaaatccc
    13441 atatgctaaa attgattagc aaagaaggag gttatattat gagttggtta tggacattga
    13501 ttgttggagc cgtgatcgga gcaattgcag gtgcgatcac cagtaaagga aattcgatgg
    13561 gctgtgtaac gaatatcatt gctggactag tcggttcaag tattggacaa gcgattttcg
    13621 gcgaatgggg accaaaagct gcaggtatgg cacttattcc atctatttta ggcgcagtga
    13681 ttttggtagc agttgtctca tttttctttg gaaaacgatc ataagttttt acaaaatagt
    13741 aaactaaaag gagttgcagc gagactgtga ctcctttttg gctttattta tttaaaataa
    13801 aaagaagttt tccggaaatt tttatttaac tgtcgtgtac gaagaaaagt ttgctataat
    13861 ggaaaaaagc gtgcaaagaa agagggagtt tcattatgat gctacgtgta gaaaaattgc
    13921 gcaaaaaaat gcaagaagaa aatttggatt catttttggt taccagtccg tacaatcttc
    13981 gttacttaac gaactttaca ggtacgacag gattagctgt tattactttg gaaaaagcat
    14041 tttttattac tgattttcgt tataccgagc aggcggcagc gcaggctcaa gggtttgaaa
    14101 ttattaaaaa tgtcggacct atttttgatg aggtagcaaa cttagtacgc aaagaagaac
    14161 tgcactcatt agcttttgag gaaacaacag tctcattttt agaatattcg gttttagaag
    14221 aaatcatcga tgcggaatta gtacctgttt cacagatgat cgaagaactc agagaagtga
    14281 aagatgaaga agaaatcgct attatcgaaa aagcatgtca tattgcggat atggcatatg
    14341 atcacatcct gaaaatgatc caaccgggca tgactgagat tgaagtcgca aatcaactag
    14401 acttttatat gcgttctctt ggcgcttcag gtgtttcttt tgatacgatc gttgctagcg
    14461 gattgcgttc ggctatgccg catggtgtag caagcaaaaa aatcatcgaa caaggtgact
    14521 tgatcacatt ggactttggt tgctattacg aagggtacgt ttccgatatg acacgtacat
    14581 tcgctattgg tgatcctggg gaaaaattaa aagaaattta tcaaatagtc ttagatgctc
    14641 aattagcagt tattgatgct gcaaaaccag gaatgactgg tgtccaatta gatgcagttg
    14701 ctagagatta tatttcaaaa catggttacg gggaagcatt tggacattct acaggacatg
    14761 ggatcggttt agagatccac gaaggaccaa acgtttcttc tcgtgcagaa aagcaatttg
    14821 taccaggaaa tgtgatcact gatgaaccag ggatctactt accaggaatc ggtggagtac
    14881 gtattgagga tgacttgttg atcacagaag atggaaatcg tgtgttgact cgttcaccaa
    14941 aagaattgat catcttatag tttcatcaat agaagaagta agcgtaaaaa tcagtgttca
    15001 cagactttgt tatagaaaat cactcgattt aatgatacac tatagaagaa cttttaagct
    15061 gtaagagata ataaagttgg gtgttttctc caattcatag cagtttgcag ctgatcggcg
    15121 gcagttttct taccacgtga tcactatgcc gcatcataga gaattcggat gtatctcgtg
    15181 cttaggcaaa gggaggaata aataaatggc agatgaaaaa aatttgattt taaacgcaaa
    15241 ccaagattta ggagagatcg tgatcgctcc tgaagtcatc gaagtaatta tcgggattgc
    15301 agcttctaaa gtagaaggcg tatacggaat gcgcggaact tttgccagca atgtcactga
    15361 attattagga cgtgcagctc acggaaaagg tgtttactta gcaaacgagg aagatggctt
    15421 gaaagtagat ctttactgtt atctagaata tggtgtttca gttcctaaag tagcaatgga
    15481 aatgcaggaa cgggtcagcc agcaagtcct tttcatgacg gatatcgatt tatctgaagt
    15541 caatatccat gtagtagctg ttgttcctga aaaattagaa caaccaacaa tcgaagaact
    15601 ttttgaagac gaagaggaag agaatgaata aagaattatc aagacacgag attcgggaaa
    15661 tggcattgca ggctttattt ccattagact tcaatgcaga cctaacaaaa gaagatgcga
    15721 ttttcaacgc aatcgaatta gaccaccgcg acatgatcaa cgaagatgaa tcagagtttg
    15781 ttcctgttta tcttgatacg ctagttggcg gtgtctgtgc aaaaaaagat gaattggata
    15841 aagtgattga aaaacattta aaaaacaatt ggcacatcaa tcggatctca aaaatggatt
    15901 tggtgatctt gcgtatcgcg atttttgaaa tgacttatgt gacagatgta ccagcggctg
    15961 ttgctttaaa tgaagcaatc gagttggcaa aaacatttag tgatgaccgt tcacgtaaat
    16021 tcgtcaacgg tgttttatcc aatgtattga aagaattaga agctggggcg taagtcctag
    16081 cttttttcta tgaatcatca taaacctgtt ataaatcggg aaaattctat ggtagaatga
    16141 aaacacaaaa aatatctggg agtggttaga tggcagaatt gattaacgga aaagaattag
    16201 ctgaaaaaat gcaggcagag attgctgaaa aaatccaaaa attgaaagat aatggtatcc
    16261 atcccggatt agtcgtttta ttggtagggg aaaatccagc cagccaaatc tatgtaagaa
    16321 ataaagaacg tgtagcaaaa gaaatcggaa tccattcatt ggtagagcgt tatcctgaga
    16381 cgatttctga agacgaacta cttgcagaaa ttgaaaaata caatcaagat cctagttttc
    16441 atggtatatt ggtccaatta ccattgccaa agcatataga tgaagaaaag atcctgttag
    16501 ctatccgccc tgaaaaagac gtagacggtt ttcatccgat gaatttaggc agactccttg
    16561 cgggtaaacc ggacaagatt ccttgtactc catacggcat catgaaaatg tttgaagctt
    16621 attctatcga tccggcgggt aaacgagcag tagttatcgg acgaagcaat atcgtcggta
    16681 aaccgatggc tcagctactc ttgatggcag atgcaactgt tactgttgct cattctaaaa
    16741 ccgaaaatct ggctgaactg gcacaagaag cagatatttt agtcgtagca ataggacgtg
    16801 gacattttgt cacaaaagaa ttcgtaaaac caggtgctgt agtgatagat gtagggatga
    16861 atcgtgatgc taacggtaag ctgatcggtg atgtgaaatt tgatgaagtg gagcctattg
    16921 caggctggat cactcctgtg ccaaagggtg tagggccaat gactatcacg atgctgatgt
    16981 atcaaaccgt aaaatctgcc gaagcttttg gagcaggtga atagatggca caagaatatt
    17041 taactgtcac tacattaacg aagtatttga aaagaaaatt tgatgccgat ccttatttag
    17101 aacgcgtata tttaacaggc gaaatatcga atttccgttt acgtgccaat gcccatcaat
    17161 atttcagctt aaaagatgac catgcaaaaa tatctgctat tatgttcaaa ggagcattcc
    17221 aaaaattacg attccagcca aaagaaggaa tgaaagtcct tgtcgttggg cgtatttcat
    17281 tatatgaagc gggaggttct tatcaaatct atgtagagca catggagcct gatggaatag
    17341 gtgctttata tcaagaattg gaagaacgaa aagaaaagct gatgaaggaa ggcctttttc
    17401 aagggcctaa aaaaatgctg ccacgtttcc caaagcgaat cgcggtcctt accagtccaa
    17461 gcggagcggt tatccgcgat atcatcacga cggtgaaaag aagatatcca attgtccaat
    17521 tggttttgtt cccaacagtt gtacaaggaa accaagcagc ggacaatgtt gtcaggaata
    17581 tccagagagt agaagagatg ggcaatttcg atacaatgat catcggtcgt gggggtggtt
    17641 cgattgagga tttatggccg tttaatgaag agcgggttgc tcgggcgatt tatcaagcta
    17701 atacgccggt gatttcatct gtaggacatg aaacagatac cacgatcgct gatttagtag
    17761 cagatgttcg ggctgccact ccaacagcag ctgcagaatt agctgttcct gttttgtcag
    17821 aagagttgat gcgcattcaa gaacggcaag ctagattgca gcaggcgttt gtacgccaga
    17881 tccagcgcaa acaagaacgg ttcgagcgtg ctcgacgttc ctatgtattt cggcagcctg
    17941 aaagattgta tgaaggtcag accatcaaat tagaccagtt gaaacagcga ttatatcaag
    18001 ctacacagca aatttatcat gcgaaagaaa aacaaacgat cgcattaagc caccaattgg
    18061 aacaagtggc tccgctttat cgagttaaag cagctcgcca agaaactgct tatctagaaa
    18121 aacgcttaga agagaaaatc caacaatata tgaaacaaca gcagcagcgt tttgctcaag
    18181 ctgttcaatc tcttgatttg ttgagtccat tgaaaatcat gggaagaggc tacagctata
    18241 cgacgttaaa tgatcaagta gtaaaaagtg tcaccgagat tcatcaaggg gatttgctga
    18301 cgatccatta tcaagatgga atcgttaaag gacaagtaca agagatagac aaaggagaat
    18361 aaaaatatgg aaaatccaac ttttgaagag tcactgcaag aattagaaaa aattgtcatg
    18421 cagttagaac aaggagacgt tccattagaa tcagctctag attcgtttaa acgaggaatg
    18481 gaattaagca aacattgcca agatacatta gctaaagcag aagagacctt gacgaaaatg
    18541 atgacagaat caaatgaaga agttgatttt gacggaaacg aggaaagctg agatgagatt
    18601 aactgacttt tcagctattc atctacctgc tgtagaaaaa gaaatcttgt cttttttaga
    18661 tgagcataca acggatagag gactctatga tgcgatgagt tattcagtga aagcaggcgg
    18721 gaaaagaata cgtcccttat tacttttaac ggccgttgct tcctttgatg agcctattga
    18781 tgttcccgtc tatcaagtag cagctgcttt agaaatggtg catacgtatt ccttgatcca
    18841 cgatgatttg cctgcgatgg acaatgatga tcttcgtaga ggaaaaccta ccaatcataa
    18901 agtattcggc gaagcattag ctatattggc cggagatggt ttgcttacag gagcattcca
    18961 gctaatcagt atggcacact taggtaattc tcctaagctt ctgctgcttc agcaattagc
    19021 tgtttgtgcg ggatcacaag gtatggttgc tgggcaagct gcagatatcg agggcgaatc
    19081 gaaaaagctt tctttagaag aattagcatt tattcatgaa agaaagacag ggcatttgat
    19141 tcgttatgcc ttgctggctg gtggcatatt agcgaagcaa cctgaagaaa tcttattgct
    19201 acttcaacga ttagccgaac atttaggttt agcttttcag ataagagatg atctactcga
    19261 tgtgattggt acaacgaaaa cattagggaa aacagctggt aaagatgaac gtatggaaaa
    19321 aaatacttac ccgcgtttat taggcctaga aaagacacgt gaggcgttgg agattgagct
    19381 gttatccgcc aacaagatca tagacaaatt agaagaggac gttttttctt ttgatggcgg
    19441 gctgatgcgc cagatgatca agcaatttga tgtggagaaa tgagggaaag aatggaaaaa
    19501 gaacgcgtag atgtattagc ggtcaaacaa ggattatttg aaacaagaga acaagccaaa
    19561 cgttcagtga tggctggttt gatttacaac gaaaaaaacg agcggtttga taaaccggga
    19621 gaaaaaattc cagtgagtag tgaattaaga gtcaaaggta aaaaacttcc ttatgtttct
    19681 cgagggggat taaaattaga aaaagcactc aaagcatttg atctatctgt tgaaggaaaa
    19741 acattgttag atattggtgc atccactggc ggatttactg atgcagcttt gcaaaatggc
    19801 gcaaaaatga gttatgcttt agacgttggc tacaatcagc tagcttggaa aataagacaa
    19861 gatcctagag tcgtcgtaat ggaaagagtg aattttcgtt atgcaaaacc cgaagacttt
    19921 accttaggaa ttccagaagt tgctgttatc gatgtttctt ttatttcact aaaactgatg
    19981 ctgcctccat tacatgctat tttaaaagaa gagggagaag tgattgcttt aatcaaaccc
    20041 caattcgaag caggcagaga agctgtaggg aaaaacggaa ttgttcgtga ccctcagacc
    20101 catcaaaaag ttttagaaga cattttgtca tttgcggcac aaggtggata tgatgggctg
    20161 gaattaagtt attctccgat cacgggggga gaaggaaata tcgagttctt ggctcacttg
    20221 aggaaagtac ccgaaagcgg aacaatcaat tcagctatta atatggcaga agtggtttca
    20281 aatgcacacg aacagtttga ccataaataa attgtaactg tgaaaatcaa gtgagaagta
    20341 tgcggattct tctttattat accaaggaaa taagctatca ttgattaaga agaatatgct
    20401 aaggagcgaa gacgtaatga gaaaaaaaga tcggcatcgt ttgattactc gattgttgag
    20461 tgagcaagat atccgaaagc aggaagaatt tgttgaaatc ctgaaaagca aaggaatatc
    20521 tgttactcaa gctacgattt ccagagatat aaaggaactg aaactgatca aggtaccggc
    20581 ttcagatggc ggctaccgtt atagcatacc agctgaaaca aacgaagatg taaatactaa
    20641 attggaaaaa ctattaaaag acgctttcgt ggcagttgac caaatggaaa aatttgttat
    20701 tttgaaaaca ttgccgggaa atgcttcggc agcagcaaat ttgattgata aacgttacaa
    20761 gaaagaatta ttttcgatta tcaatgatga cgataatgtt ttgatgatta cacgaactga
    20821 agaccaggca gttttattga agaaagactt cctgcattac ctttaagttg aggagcgaat
    20881 gtgatgctac aagaaatcag tattactaac tttgcgatta ttccggaatt gcggctttct
    20941 tttcacgaag gaatgaccgc gctgaccgga gagactggtg cgggtaaatc cattattatc
    21001 gatgctttag gtctgttagc aggaggaaga ggttcaagcg attatatacg tcaaggagca
    21061 gaaaaatgtg tgcttgaagg gttgtttgaa ctaccaaaac aagaaggatt ctctgaactg
    21121 atggttgaat tagggatcga aacagatgag gacaatctga ttgttcgacg ggatatgtcc
    21181 ttaactggaa aaaatgtttg tcgagtgaat ggtcatatta tcacattagc taatctgaga
    21241 agaatcggca gttatttagt agatatccaa ggacaaaacg aacatcagga actgctccag
    21301 cctgaatcac atcttgcttt attggatcgt tttggagatg tagctttcca acagaagaaa
    21361 aagagttatc agcaagaata tatctcttat cgtgagctgg aaaaaagggt acgaaaagtg
    21421 cagcaaaatg aaaaatccta tgttcagaga atagatatgc tccatttcca gcaagaagaa
    21481 atagctgctg ctgatttgca agtcggagaa gaagagaaat taaaagaaga aagagaaaaa
    21541 cttagcaatt atcaaaaaat tgctgatgga ttagctgcag gttacggagc actgacgaat
    21601 aatgaacaaa acagtgtaga tggtgttggc ttggccgttt cagaactgca agggatcgcc
    21661 catctagatg tggagtatga agcaatttat gaaaatatcc aaagtgctta ctatttgctg
    21721 caagatgcaa tcggcgatat gagtcgtcag attgatttat tggagttaga tgagagtcgt
    21781 ttagaagaag ttacgcaacg tttagaatta atccgacagt tgaaacgtaa atacggcgaa
    21841 tccattgagt caatattggc ttactatgac gaaatcaccg aagagctagc ttcttctgat
    21901 ttttcagaag gtcaattgga caaaatgaaa gaggaactag agcaaaaaga gcttctactt
    21961 cagcaacaag ccgcagatct tcatgaagcg cgtaaaaaaa tcgcaaaaga attggaaaaa
    22021 tccattctgc atgaattgaa aagtctgtat atggaaaaca ctgagtttga ggtacgtttc
    22081 ttaaaagaag aaaatagaca attgaatcat gatggatttg accaaatcga attttacatt
    22141 acgaccaatc caggagagcc actgaagcct ttggtgaaag tcgcttctgg tggcgaactg
    22201 tctcggatgt tgcttgcttt gaaaactatt ttttcttctg agcaaggtgt gacaagtatt
    22261 atttttgatg aagtggatac aggtgtgagc ggaagagttg cacaggccat cgcagataaa
    22321 atttctcaaa tatcgaaata ctctcaagtt ttgtgcatca ctcatctgcc tcaagttgct
    22381 gcagtcgccg actatcaata ttatattgtg aaagcagtga ttgatggacg tacgcaaact
    22441 tctgtttcag agttgaaaac aaaagaaaga gaagaagaaa ttgcacgtat gctggctgga
    22501 agtgagatta caaaactcac actcgaacat gcaaaagaac tattgaaact cgcaaaaagg
    22561 acctgagcca cctcgcagtg gttcaggtcc ttttcagcag ctgatagcta tattttattt
    22621 ttacatgaca ctcattacag cagtcaatac aacagatcca atcgttaaaa tcagcattgt
    22681 ccaaatgaca aattttgtta ctttagcaaa agtacttgtt tgtttttttt cacgcattgt
    22741 tttcaacctc gtcattaatt agttgattca agtgtacaat cttttttcca tttccacaag
    22801 agggcttttt tataaaaact tccctttttt taaataatag gtagttacga tacaaataaa
    22861 taatgttaat agaactagcc atataaaggc atcatccctt tcagcaatag gtaacttaat
    22921 gttcattcca tagatactgc cgacgattgt cggtatagtt aaaataatcg ttaaagaagt
    22981 taaaattttc ataatattat tcagattatt cgatacgatt gctgaaaaag tctgattcat
    23041 ttgttctact agttttaatt gaatgcgaga agtagtcatt gcctgctctg tttccaccaa
    23101 tacatcacgt aaatgatttt tcaaagcatg attttcttgg aaaagtttat tttgcgctag
    23161 ttctttcaat gcatctaaat tagcagaagt cgcttctctg aaataaacga ggcttttttg
    23221 gatatccatg atttgataaa gttgtttgtt ttcagtggat acttgaagtt tggcttccaa
    23281 agtatcgcat tgagattgaa gcgtagctaa ataactgttg taagagagtg tgacctgcca
    23341 aatgaattga aagaagatat tgagattagg cgaaatatca ctttttggaa aggtctggct
    23401 aaagactttc tttaaaaaca gtggttcatg attcgtaatt gtgataagct tctttttggg
    23461 cgtcaaaata atagagagcg ggtatgtatc cacttgaaaa taaccgctag gacttgtttt
    23521 agcaaaagga tattgtaaca ataataacgc tggctttgtc aactttgatt gattgaattc
    23581 ttctgtacgg gaattttctg catcgtccaa tatggaagtg atgtaatctg acggaatttc
    23641 aaattgatgt gtcagctgat gaatctcttc ttgattagga cgttctacac aataccagat
    23701 cgtttggtca gtttcctttc cggtattacg cagtttctca ttatctaatg taaaatattt
    23761 gatcatgatt ttcctcctgt tgcctgtttc ttttattata acggataggg aattggaata
    23821 catggaatgt gtttgtaaga tatagaatat aactgcataa atttaaataa aatgaaacag
    23881 ttgtgtaaag tatatacagt atacttgaag caacgataaa aaaggagttt acgttcatgg
    23941 gcacattcac accacttatc ttgttttggt atatttaccc ggtaatcgtt ttgtttggtt
    24001 gccaatttct tgtaaaatta ttttcgttaa atcgtcgttt taaaataaaa gcccctgatt
    24061 tggcgattcc ttttttgtgg ggcggtatgc atgcattgtc tagaaataca gggacaattt
    24121 ctatcttacc gtttttcttt atttctgttt tgctaatggg aatttgtatt gctgttttcc
    24181 aagcatatta ttatgaagaa attatttatc cgcgttattt caagatgact tggcgtttgg
    24241 tttttctgtt gacaatcgtc ttgtacgctg tcttgatcat tgtgaacata ctttcttacc
    24301 tatgaaaaaa atagaaaagc tgtaaaaagt cttttaaaag gctttttgca gcttgatttt
    24361 taaaaaaatg tttatgaata aaaaaagttg ttcccggatt tcccagaaaa ggtggtagaa
    24421 agtgggggat tgtggtagac taattttagt tagtggagga atggggggct ttcaatgttg
    24481 atgggtgaat tccaacataa tattgatgca aaaggacgtt taatcgtgcc ctcaaaactt
    24541 cgtgaagaac ttggcgaaaa gtttgttctg acacgcggat tggatggctg tcttttcggc
    24601 tatccgatga gcgaatggga aaatctggaa gctaaactga acgaaatgcc gttggctaaa
    24661 aaagacgcac gtacttttgt gcgattcttc tattcggctg ccactgaatg cgaacttgat
    24721 aaacaaggac gaatcaatat acccagcacg ttaagaaatt atgcagcttt gacaaaaggt
    24781 tgcgtagtca tcggtgtatc taaccgaatt gaaatttggg atgagacacg atggcaagat
    24841 ttttctgcag cagcagaaga gaactttgac gaaattgctg aaagtatgat tgatttcgga
    24901 ctataagaat aggaagaggc tgactaatga ctgaaacatt tcaacactat acagtcatgt
    24961 tgaaagaaac ggtagatgga ttgaacatca aaccggatgg cgtttatgta gactgtacac
    25021 tcggcggtgc cggacatagt gaatatttat taagccaact aagttctaaa gggcatctgt
    25081 atgcgtttga ccaagatcaa aaagcaatcg atcacgccaa gctgcgttta gcttcttatg
    25141 tcgaacaagg tatggtcaca tttatcaaag cgaacttcag agaattagaa gaacgcttga
    25201 gtgagcatgt agataaggta gacggcattt tgtatgatct aggggtttcc tctcctcagc
    25261 tagatgaagc agaacgtgga tttagttatc atcaagatgc accgttggat atgcgcatgg
    25321 atcagtcagc tcctttatcc gcttatcacg taattaatga atattcctac catgaattag
    25381 taaagatttt cttccgctat ggcgaggaga aattttccaa acaaattgct cgtgaaatcg
    25441 aacgagtacg aaaaacaaaa ccaatccaaa caactggtga gcttgtcgaa atcattaaac
    25501 aagtcatccc agctcccgca agaagaaaag gcgggcatcc tgcaaaaaga atttttcaag
    25561 ctgtgcggat tgcagtaaat gacgaactcg gtgccgaaga ggcctcttta gaacaggcaa
    25621 tccggttatt gaaagtcaat ggaagaatca gcgtaatcac attccattca ttggaagaca
    25681 gaattgtcaa aagtatgttt aaagaatata gtacggtaca agatctacca ccgggacttc
    25741 ctgtcatacc tgacgaattc caaccagaac tgaaagtcat tactcgtaaa ccaatccttc
    25801 cttcagagga agagctaaca gaaaacaatc gatcacgcag tgcaaagtta cgtattgcag
    25861 aaaaaataag aatgaaagag gagtagaatg atggcagaat taaagaaaat gcaagattac
    25921 gaatatgatt tacctgaatt ggtggatcag ccggaacaac aaatgcctga ttcttcttcg
    25981 aatcaagaaa tcattcttcc acaatcaccg gcaaaacgat taaagcagat ttctgctttg
    26041 gaaaaaattg ctgtggcaag cgtggcgatt gctattgttg ctttatgtgt tttaacagtt
    26101 atgctgcgca caaatatcag cggagctgaa aaagaaatca cgtcaataca aacagaaatc
    26161 aatgataaaa agcaagaaaa aacgagttta gaacaagaaa aaaacgaact ttctcgtact
    26221 gaacggatta aacaaatcgc agaagaaaaa ggattgtcta ttaatgacga caatttaagg
    26281 aaagtgaagt aaatgagtct taaaaataaa attcggcggt ttatggaaaa gaaaaatcta
    26341 aatccgatga acaatcgcaa aaaagtaggt atcattttat ttgctacgag tattggattg
    26401 ttctttttat ttgccgtccg tttctcgtat atcgttattg gaggtcatgt tgctggtacc
    26461 tcattagctg aaaaaacaaa acagttgtac caaggaagtg aagtagtaaa agcaaaaaga
    26521 ggaacgattt atgatcgtaa cggtgtggcg ctcgcagaag atgcctcctc ttattcaatt
    26581 aaagcgatac tctcaaaaac atatacctct ggggataaaa aactgtatgt tgaagaaaag
    26641 aattttgata aaatagcaga aatcctgcat aagaatcttt ctatagacaa aaaagatgca
    26701 ctgaatattt tagaggacgg cgcaaaaaaa gagctgtatc aagttgaatt tggtagctat
    26761 gggaaaaata tctcccaaga aaccaagcaa aacatcgaag cggacatgaa aaaagaaggt
    26821 gtcgctggtc tttattttgt cgaccatcag gctcggatgt atccaaatgg cgtcttttct
    26881 tctcatttta ttggatacgc agttccagac aaagatgaga atggcttagt tggtaaacta
    26941 ggtttggagt ctgcttacaa tgatatcttg agcggtaaag atggtaagat tatttatcaa
    27001 aaagataatt ttcaaaatcc tctgccaggt acagtggcag aagaagaaaa agctgtcgac
    27061 ggacaagata tctacacgac tttagacagc agactacaaa gttatttaga aacactgatg
    27121 gatcaagtaa atgaagaata tcagccagaa gaattgacag cagtcctgat gaaagcaaaa
    27181 accggagaaa tcttggcaat gggacaacga cctactttca atcctgaaac aatggaagga
    27241 ttgactggaa aagatgctat ctggcgtaat ttcttggtac aagatagtta tgaaccagga
    27301 tcaaccatga aagttttcac tactgcagct gctatcgaag aaggagaatt caacgagaat
    27361 gaaaccttcc aatctggaaa aatccaagta gaagatgcta cgatcaatga ccatgacttt
    27421 ggtgaaaaag gtgtgctgac catgcgtcaa gctctttcat ggtcaagtaa cgtcgggatg
    27481 gtcatactgg aacagcgatt aggcggacga tggtataact atttacaaaa actgggattt
    27541 ggacaaagta ctcattctgg attagatgat gaagtaaacg gtgctttacc tacattaaat
    27601 attgtcgacc gagcaatgag tgcttatgga caagcggtag gtgtgacgaa tttccaaatg
    27661 atgaaagctt tcacttctat tgcgaataac ggtacaatga ttcaacctcg atatatcagc
    27721 aaagtggtgg atccccaaac tggagaagag cgaacaactc aaacagaagt tctagggcaa
    27781 cctttttcaa aagaaacaac agaaaaagtc cgtgaatata tgagagatgt tgtcgaaagc
    27841 gaaaactacg gtagtgctta tggtgtatat agtgtacctg gatacaatgt atcggcaaaa
    27901 aacggaacag cccaaatcgc ttctgataca gggggatatc aaacaggaga tacagcctac
    27961 ctgtattcga ttgtggagat ggttccttct gaagatccag actatgtctt gtatctaaca
    28021 atgaaacacc ctaagacata tgatcgaatg gcattggcta aaatcgcaaa tccattgatg
    28081 aaacgggcaa tggattttaa agaaactgaa gaagattcag atacagaaac aaaaacagaa
    28141 aaagtatccg tagccgatta tcgtaatctt gaagcggacg ttgcagcagc tgatgctcaa
    28201 aaaagtggac tgcaacctgt agtgatcggt aatggtaaaa aagtacaaaa acaatctact
    28261 gccaacggtg atcagttgat ctctggagaa aaactgatcc tatatactgg tggtgataag
    28321 ctgatgcctg acgtaacagg atggtcaaaa gcagatatca tgaagctggg gaaaatcctc
    28381 ggtattgaag ttagttttga tggagatggt tattgtgtca aacaagaact tgctccatat
    28441 gaaaaaatca ctgaagacaa acttagtttt actttagaag aataagaaga gaaataggag
    28501 agaaaagagc atggaatgga cacaagcatt gattcccatt gtcagcagtt gtgcaatgac
    28561 aattgccgca atgccgctat ttattggtta ttttcaaatg aaaaagcaag gtcaggcgat
    28621 tcgtgaggag ggaccaaagt ggcacaatag caaagccgga actcctacaa tgggcggctt
    28681 agtgtttctg atcggctcta ttttgacagg aatttgggtt ggagcttggc aaaaacagct
    28741 aactcctact cttttcatct tgctttttgt attagcgtta tacggagtaa tcggctttct
    28801 tgatgatttt ataaaaatat tcaagaaaag aaacatggga ctgaactcga agcagaaatt
    28861 gattggacag attctagggg gaatcatctt ttatcttgtt taccgttctg aaggttatcc
    28921 aggtgtattg aatttcttcg gactagatct acctttagga atcatttatg gtattttcgc
    28981 tctcttctgg cttgttggat tttcaaatgc agtaaatctg acagatggca tcgatggatt
    29041 agttgctggc ttaggaagta tctcttttgc agcttacggt ctaattgctt ggcatcagca
    29101 tcagtatgat gtattaatca tttgcttaag tgttttagga ggtttactgg gtttcttcgt
    29161 ctacaacaga aaacctgcca agatttttat gggagatgtc ggttcattag cgttgggcgg
    29221 acttttagcg gcaatttcta taatgctaaa ccaagaatgg acattgcttt tgattggttt
    29281 gatctatgtt atggaaacag caagtgtgat gcttcaagtt acttccttta agcttacagg
    29341 aaaacgaatt tttaaaatgt ctccaattca tcatcacttt gaaatgtgcg gatggtcgga
    29401 gtggaagatt gatacagttt tttggttgat cagcattgtg acttcgttga tcacattatg
    29461 gatcgtttgg taggagatga cgggaaatga aaaaaatatc aacatatgaa aataagaaag
    29521 tgcttgtctt aggactcgca aaaagcggag taagtgcggc caaattatta catgaattgg
    29581 gcgcacttgt caccgttaat gatggaaaac cttttgacga aaatccggaa gcacaagagc
    29641 tgctttcttt agggataaaa gtaataacag gtagtcatcc aatcgaattg ttggatgaag
    29701 aattttcatt gatggtcaaa aatccaggga ttccctacag tcatccttta gtagcaaaag
    29761 cccaagaaat gggtattccg gtaattacgg aagttgagct agcttacgaa gtagcggaat
    29821 gcccaattat tgggattaca ggaacgaacg gaaaaacaac gacaacaacg atgacaggac
    29881 tactactgaa tgcaggagca gatcaaggaa ttgcacgttt agcgggtaat attgggtatc
    29941 cagcaagtgg tgtagcgcaa gaagcaaaat cagaagataa aattgtgatg gaattgtcta
    30001 gcttccaact catgggaatc acagatttcc gccctcatat agcggtgatt accaatatct
    30061 atgaagcaca tattgattat catggtacaa gaaaagaata tgtcaaagct aaatggaacc
    30121 ttcaaaaaaa catgacagaa aaagattatc tgatccttaa ctggaatcag tcagaactac
    30181 aagaattagc gcaaagaaca aaagcacgcg tactgccttt ctcgacaaaa gaagtgttgg
    30241 aagacggtgt gtatgctgac gattactcaa tatactataa aaaagaaaaa ataatggaaa
    30301 tatctgaact tggcgtacca ggtaagcata atgtagaaaa tgcactagct gctatttctg
    30361 tagcaaaatt gtatggtata tcaaatgaag ctatacgaga aacgttgcat tttttccatg
    30421 gcgtaccaca tcgtacgcaa tatgtaggag aaatccaagg cagaaaattt tataatgatt
    30481 caaaagcaac caatatttta gcgactaaaa tggctctaag tggatttgaa acgtctaagg
    30541 tcgtgttatt ggctggcgga ttagacagag gaaatacatt tgatgaatta attccttcat
    30601 taaaaggaat caaagcaatg gtcgtatttg gccaaacaaa agaaaagttg atggatgctg
    30661 gaaaaaaagc aggcatcgag acaatagtaa cagccgattc tgtagaacaa gcagttcctt
    30721 tagcattaga aaacagtaca gatggtgatg tagttttatt atcaccagcg aatgccagct
    30781 gggatcaata cccaaatttt gaaactagag gaaacagatt catggaagct gtgaaccgat
    30841 taaaatagaa aaagtagaag cgagcgataa aaaatgaaaa ttttggttac aggcggagga
    30901 acagggggac atatttatcc agctcttgcc tttgtcaatt atgtaaagac tcaagaaccg
    30961 aatgctgaat tcatgtatgt gggagcaaaa cgaggtctag aaaacaaaat cgtccctgat
    31021 acaggaatgc ctttccatac cttggagatt caaggattta aacgaaaaat ttccatgcat
    31081 aacatcaaga cggtccgatt gtttcttaag agtatccgtc aagcaaaaaa aatcctcagt
    31141 gattttcaac cagatatcgt tatcggtact ggaggatatg tttccggagc ggtagtgtac
    31201 gctgcagcca aaatgggtat tccaacagtt atccatgaac aaaacagtgt accaggtgtt
    31261 accaataaat ttttaagccg atatgtggat cggatcgctc tttcatttaa agatgcggct
    31321 tccttttttt ctgaaaacaa agcagttctt gtaggtaatc ctcgagcaca agaagtagca
    31381 gatactaaaa aatcagatat tttgaaaaca tttgatttgg atccagaaaa gaaaacagtc
    31441 ttgatttttg gcggtagtca aggagcactt aaaatcaatc aagcagtatc agttttttta
    31501 agtgaatata ttccttcttc ttaccaagtt ttgtatgctt caggagatag atattatgaa
    31561 gagatcctaa ataaaataaa tcctgaaata caaaatatta gtgtccagcc ttatatcaaa
    31621 aatatggcag aggtcatggc ttccagtgat ctgctggtgg ggcgtgctgg tgcaacttcg
    31681 atcgcagaat tcacagctct ggggttaccg gcagtgttgg ttcccagccc atatgttacg
    31741 aatgatcatc agactaaaaa tgcaatgagt cttgttcacg caggagcagc aaaaatgatt
    31801 gcagataatg aactaacagg tgaatcacta agtcagacag tcaacgaaat catgggtgat
    31861 gaagaattgc aaaagcaaat gtgccgagct tctaaagaac aaggaatccc tgatgcttca
    31921 aaacgtcttt atgacttagt aaaacaaata atcaaaaaat aaaggcggtg gtcttagtga
    31981 gtaagaaaaa agaaccaaaa aaattgtctc atccgtcttc tgatgaaaac aatttgactc
    32041 cttggcaaaa ggctaaccga aaatatctgg cagaacaaga gatgaaaaac caagagccaa
    32101 aaaaagaaga cataacatta gatacacccc tttcagattc tgaagaagaa aaaacggttc
    32161 aagaggaaca gcaagaagaa agccagtccg agaaaaaaat cgaatttttt gaagagatca
    32221 agcaaaagaa aaacggtgga ccatataacg gttctttttt gaaccggctg cccaatttaa
    32281 aaagtcaacg gcacaaagta ctatttcgtc ggttagcttt gatcatcact gtattgggta
    32341 taccgctgat ttttttgatc tattatgttt ctccttatag caaattacaa gcagttgcag
    32401 tatcaggcaa taagacagtc aacagccaag aaatcatctc tgatacaaaa ttgtcattag
    32461 gagaaaatgt ttggggacag tattttcatc gatctactta tatcgatcgc cttaaaaaag
    32521 cacagccaag aatcgaaaca gcaaatatac atttcaaagg gatgaatgaa ttcgatttgg
    32581 atgtaacaga atataaagag attgctttga tcgcaaaaaa caatcaatac tacccagtga
    32641 ttgaaaatgg aacagtatta gatgaaaaag tagcaaatcc tacaaaaaat ctccctatct
    32701 tggaagagtt caaagactct gcaaaaatca aagaattaac aaagcaatac aatcagttat
    32761 catcagaatt acaaaaagca atttctgaaa tcaagtacac gccaagagca agcaataaaa
    32821 atctgatcca attgaatatg aatgacggca atcaagttat tgtaaatatc aataatcttg
    32881 cgaaccaaat gaaatattat tcgcaagtag ccaaagatat ggatgaaaaa ggtgtgatcg
    32941 acatggaagt tggtatcttc tcgcatcctt acggtagcac tcaagaaacg gaagaatcgg
    33001 gccaatcgga agaaacaact gaaagtgaag aaaatgcgga tcaaacaaac gaatcaagtg
    33061 aagaggcaga aacgcaaaaa gcacaaacac ctggatcgtc tagcacaaac ggatcatctg
    33121 attcaaatga ttcaagtgaa acaagtcaaa tagaagataa tccaagtaca actccgtcaa
    33181 gtttaaatga aaattgataa aacaacttta aaaagtaaga atgtactttc taaaaaccag
    33241 taatttatgg tagaattgga agcagtgaat atatctatta ataagtatat tttaaaacgg
    33301 acgctagttg ttcgcttgta tatggaatag taagtaggag ggaatcccat tcatggcaaa
    33361 aacaggaatg tatgtaggcc ttgatattgg aacgacatct gttaaagttg tcgtggctga
    33421 atatatcgac agccaaatga atattattgg agtaggaaac gcaaaatcag aagggattaa
    33481 ccgaggcatt atcgttgata tcgataaaac ggtccaagcg atacaacgag cagtacgaca
    33541 agcagaagaa aaagcaggta tccagatcaa aggcgtaagt gtcggtttgc cagcaaacat
    33601 gcttgaagta gaaaattgcc agggaatgat tgcagttaac ggagattcaa aggaaatcac
    33661 cgatgaagat gtcagaaatg tggcttcagc tgcccttgtt cgttcaatac ctcctgaacg
    33721 tcaaatcgtg tcaatattgc cacaagattt tactgtcgat gggttcgagg gaatcaaaga
    33781 tcctcgtgga atgatcggcg taagattaga aatgtacggt ctgttgttta caggacctaa
    33841 aacgatcatc cataacatac gtaaatgcgt ggaaaatgcc ggattaatcg tcaacgaaat
    33901 ggtgattact cctttagcgc tgactgaatc aattctttct gacggagaaa aagattttgg
    33961 tacgatcgtc attgatatgg gtggcggcca aacaacaacg gctgttatgc atgataagca
    34021 attgaagttt accaatcttg accaagaagg tggcgaattt gtcactaaag atatctctat
    34081 tgtattaaat acgtcattta acaatgcaga agcgttgaaa ataaactacg gggatgcata
    34141 cccagaacgg acatcaccag atgaagaatt tccagtagat gtgattggtc agtcagaacc
    34201 agtgaaagtc gatgaacgct acttgtcaga aattatttca gctcgaatgg aacagatttt
    34261 caacaaagca aaagatgcat tggaccaaat cgaagcatta gagttgccag gtggcgtcgt
    34321 attgacaggc ggagctgcta gtcttcctgg tgttgtggac ttggcacaag aaatcttcgg
    34381 cgtaaatgta aaattgtatg ttccaaatca catggggctg agaaacccgg tatttacaaa
    34441 tgtaataagt atcgtggatt attcagcaaa cttgagtgaa gtgtatcaat tggcaaaaat
    34501 tgctgttacg ggtgaaacct cagcagctcg tcaaatggta gttgaacaag aaacaacaaa
    34561 cacatatgag agctatgaag cacctgaaga aacaatctac gatgagccgg aaccaaaaga
    34621 gtccggagaa aatgtaaaaa ataaaatcaa gggtttcttt acaaatattt ttgattaatt
    34681 catcctggga ggaataagat atggaattct caattgataa taatatcaat gacggcgcag
    34741 taatcaaagt catcggagtc ggtggtggcg gtggaaatgc tgtaaaccga atgattgaag
    34801 aaaacgttaa aggtgttgaa ttcattacag ccaacacaga cgtgcaagca ttaaaaaatt
    34861 caaaagctga aacagttatc caattaggcc ccaaatatac tcgtggttta ggtgctggtt
    34921 ctcagccaga agttggacaa aaagcggcag aagaaagtga acaatcctta cgcgaagctt
    34981 tagacggtgc agatatgatt ttcatcactg caggaatggg tggaggaacc ggtacaggtg
    35041 ctgcaccaat cgttgcaggt atcgcgaaag aattgggcgc attgactgtt ggtgtcgtga
    35101 cacgtccatt cacttttgaa ggaccgaaac gtggccgttt tgctgcagaa ggtatcgctc
    35161 gtttgaaaga aaatgtggat acattattaa ttatctcaaa taaccgttta ttagaagttg
    35221 tagataaaaa gacaccgatg cttgaagctt tccgtgaagc agataatgtt ttacgacaag
    35281 gtgtacaagg tatctctgat ttgatcacag cgccaggtta cgttaacctt gactttgctg
    35341 acgtgaaaac agtgatggaa aaccaaggta cagcattgat ggggatcggt gtggctagtg
    35401 gagaagatcg cgtagtagaa gcaacgaaaa aagcaatctc ctctccattg cttgaaacat
    35461 ctattgatgg agctgaacaa gtacttctta acatcacagg cggtttggat atgacattgt
    35521 tcgaagcaca agatgcttca gatatcgtag caaatgcagc aactggcgat gtaaatatca
    35581 ttctaggtac atctatcaat gaagaaatgg gcgatgaaat ccgtgtgacg gtcatcgcaa
    35641 caggtatcga tgaatcaaaa aaggaacgca agtctagccg accagctaga caagcacaaa
    35701 tgcaatcacc tgctcaaaag actgtattag atatggatca agctaaacca acttcttcag
    35761 aagaagaaaa cagttttggc gattgggata tcagacgtga acaaaatgtt cgtcctagag
    35821 ttgatgattc caattttgac aatatcgaaa aaaaagaatt tgatacattc aaccgcgaag
    35881 aaacaaaatc tcatggcgat gatgaattaa atacgccacc gtttttccgt cgtaaaaaat
    35941 aaaggagagg aagagcacat gattgctgac aacttgcaga aaatcaagca agaaattcag
    36001 gattcgtgtg ctcttgttca tcgtgatccg aaagaagtta cattagtagc tgtaacaaaa
    36061 tctgttgaat ccgctcaagc tgaagaattg gttagagctg gcgttatgaa ttgtgcagaa
    36121 aaccgagtag ataaattatt agaaaagaaa caagatttat ttgattttcc tgaaataaag
    36181 tggcatttaa ttggtaattt acagcgcaga aaggtaaaat tgattgtaaa tgaaattgat
    36241 tactttcatg cgttggacag tttaagatta gcagaagaaa tccagaagcg gttagagaaa
    36301 gaattatcct gttttattga agtaaatgtt tctggtgagg aatcaaaaca tggaatttca
    36361 ccagatgagc ttttaccgtt tgtggaatct ttagctgcct gcacaaaaat caaggttgtt
    36421 gggttgatga ctatggcgcc taaagaagct tctgatgagc aaatccataa agtgtttggt
    36481 acgctgaaag aattgcgtga tcttgttcaa gcaaaaggtt atgcccatgc accatgtagt
    36541 gaattaagta tggggatgag tcaagacttt caaattgcga ttgaagaagg tgcaacattt
    36601 atccgtgtag gaacagcgct attcaaagac gaataaagga ggaacaaaaa tgccgctttt
    36661 aaacagagga gcaattgcta attttttcgg attagctgat gaggaagagt acgaatacca
    36721 agattatcct gaaacaacaa aaaaggctgt acagccaact aaaaaaaata caacacagaa
    36781 tcaatcaact cattctgtat ctgctgcgaa tactcatgaa aaacaaaaaa cggcgacttc
    36841 acgtcctgta acgcatgaaa gtattcagcc ggctgaaaag aaaaaagtaa ttgaactaca
    36901 tcaatcacct acaaaaactg tagcgaaaga aagaacagct tcaagaacct ctacacaacc
    36961 taaagcaaat cttgctggaa aaatcactgt aatggaacca agagcttatt ctgaagctat
    37021 ggcgattgct aaacgaatca ttgctggaga agcaacctta gtgaattttc atttagtaga
    37081 agaaaaacaa gcgagaagaa tcgttgattt tctaacagga acagtttatg cactagatgg
    37141 agatatcaaa cgtgttggag atgagatttt tctttgtaca ccgactggga tggagattga
    37201 cagttcaacc gctcaatctt ttgcagatgc gaatttgttt gatctgtaag aggaggaact
    37261 acgatttata ctattgcact attacttcat ttacttcaac gggcagccta tttctatagc
    37321 attatcttga tggtatatgc attgctctct tggtttccag gcgggtatca atcagggcta
    37381 ggacgattat tagctaagat ctgcgaaccg tatatcagtt tgttcgatcg tctgcctttg
    37441 aggattggac ccttggattt cacaatttta ggggcaatcc tgatcttgaa cttagccgta
    37501 caaggtttag cacagatcgt tatatggata actgtccatt tagtttattg attgaagcaa
    37561 caaaatcttt attctcagaa tagataggag gggaaaattt tgatgcaaat gtataccagc
    37621 actttcgggc agatgaacgt acatttatcg attcggtagg cgattggatt gaacaggttc
    37681 aaggacaata tgcaccctat ctgaccgaat ttcttgaccc aagacaatcc tatattttag
    37741 aaacactgat tcgacaaagc agtgatttgc gtttcatgtt ttatggcgga tatgaacagg
    37801 cagaaagaaa aagatgtttg attttccccg attattacga acctgtagag gaagattttg
    37861 aaacagcatt gtatgaaatc cattatccta gcaaatttgc cagcctaagt catgggaaaa
    37921 tccttggaac tttagtggga acggggatca aacgctctta ctttggcgat atcatctcgg
    37981 acggtgatcg ctggcaagta tttattgcaa atgaggtgga tcattttgtt gctgcacaag
    38041 taacaaagat tggaaaggtt gctgtacgct tggaaccaat caaatacaca gaaatgatta
    38101 tgccgaaaga tagctggaca caagaaagag gaacagttag ttccatgcga ctagacagtg
    38161 tgatctctga gatttataat atttccagac agcggtccaa gcagctgatc gaagctggta
    38221 aagtaaaagt gaactggaca gaaaatacaa gacctgattt tgtacttgaa ctattagata
    38281 ttatttcagt cagaggttat ggcagagtcc agatccaaga acttgagggg aagacgaaaa
    38341 aagaaaagta cagggtactg ttcggtgtat tacgtaaata aaataaaaag gtggtttact
    38401 catggcttta actccattag atatccaaaa taagacattc ccgacaaaaa tgcggggata
    38461 taatcaagac gaagtagatg atttcttaga tttagttgtt cgtgactatg aggaattgac
    38521 tcaaagaaat cgagaattag aaaaagcagt caaacattct gaagaaaaac ttgagtactt
    38581 caatgaatta aaagatgctt tgaaccaatc aatcatcgta gctcaagata cggctgataa
    38641 agtaaaaaca agtgcaagca aggaatccga agtgatcgtt acttctgcac aaaacaaagc
    38701 agatgagtta gttgcgaacg ctgaaaaacg tgcacatcaa ttaacaactg atgcagaaga
    38761 aaaagcaaga aaaatcttga cagatgcaac agaaaaagca agacagcttg cgacagaaac
    38821 tgaagatctt aagaaaaaaa cacgagtatt ccatcaacgt atcagcttga tgcttgagtc
    38881 tcaattagaa caagtgaaaa gtccggaatg ggatgaaatt ttacaaccat tctccagcta
    38941 cgtgactgac agccatgaag tgatcaaaga agttttagca aaacagcttg acaatgaaaa
    39001 tgatactgat gtaaactcag acacaggagt catagaagca cctatgacgg catttgatac
    39061 acaggctatt gatatctcga tcgtgcctgg tgaaaatgaa aacgagtaga acagaaaagc
    39121 actttgttat tcttaaagcg agccgatgag agtggaagtt cggtaagaca ctgcatggtg
    39181 aaagatcctc tacttgagac ctgcttttcc gaaattagag taagaagcag cggctgacct
    39241 cgttattggt cacagagagt ttgcactaga tagatgcaac taaatttggg tggtaccacg
    39301 gcatgttcgt cccttgcggc tgaacatgct tttttgtgtc cggaaaacaa ataaaggagt
    39361 gtatggttga atgaaaatga aagaaacctt gcaattagga aaaacaaaat tccctatgcg
    39421 tggtaatttg ccaaaccgag aaggcgagtg gcaaaaagaa tgggaagaaa atcgaatcta
    39481 ccaaaaaaga caagagttga acgaaggaaa acctagcttt gttcttcatg atggacctcc
    39541 atatgccaat ggtaatatcc atttaggaca ttcattgaat aaaatcagta aagacattat
    39601 cattcgttca aaatcaatgt ctggctatcg tgcaccatat gttccaggat gggatacaca
    39661 tggattgccg attgaacaag tattggctaa taaaggcgtg aaacgtaaag aaatgtgcat
    39721 ggcagaatac ttggaaaaat gtcgtgaata tgcactttcc caagtagata agcaacgagc
    39781 agatttcaaa cgcctaggag ttgcaggaga ttgggaacat ccttatgtta cattagatcc
    39841 ttcttatgaa gctgcacaag tacgcgtatt tggtaaaatg gctgaaaaag gctatattta
    39901 caaaggattg aaaccaattt actggtctcc gtcaagtgag tcttctttag cggaagctga
    39961 aattgaatat aaagatgtaa aatctccatc tatttttgta gctttcaatg tcaaagacgg
    40021 caaagatatt cttgatgaag atacagcttt tgtcatttgg acgacaacac catggacatt
    40081 gccagcaaac caaggtattg ctgtaaatcc aaaatttacg tatgtattgg tggaagccga
    40141 tggccgtaag tttgtcgtag cgaaagattt gctagaaaca gtccaatcag aaatcggttg
    40201 ggaaaatgta acagttctaa aagaatttgc tggacaagaa atggaatata tgactgcaag
    40261 tcatccattc tatgaacgtg aatcacttgt gattttaggc gatcatgtta ctttagacgc
    40321 tggtactgga ttagtccata ctgctccagg acatggggaa gatgactata tcgctggtag
    40381 caaatataaa ctatctgtag tgtcacctgt agactcaaaa ggattgttta cagaagaagc
    40441 acctggattt gaaggtatct tttatgacaa agcaaatcct atgatcactg atctattaaa
    40501 agaaaaagga gctttgctga agctagattt ctttactcat agttatcctc acgattggcg
    40561 gacgaaaaag cctgtgatct atcgggcaac tccacaatgg tttgcatcta tcgataaatt
    40621 ccgtcagaat attttggatg aagtagaaaa agtagagtgg gttatcccat ggggtaaaac
    40681 acgtttgtat aatatgatcc gtgaccgcgg cgattgggta atttcccgtc agcgtgcatg
    40741 gggagtacca cttccgattt tctatgctga aaatggagaa gcaatcatca caccagaaac
    40801 aatcgaacat gtggcaaaat tatttgctga acatggatca attatctggt ttgaaaaaga
    40861 agcgaaagaa cttcttcctg aaggatttac ccacccaggt tctcctaatg gcgaatttac
    40921 aaaagaaaaa gatatcatgg atgtctggtt tgattctggt tcttctcatg aagctgtttt
    40981 acgtcagcga ccagaattat cattcccagc agatatgtat ttagaaggat cagaccaata
    41041 tcgtggctgg ttcaactcaa gtatcacgac tagtgtggct atcaatggtg tagcaccata
    41101 caaatcagtt atttctcaag gatttgcttt agatggtgaa ggccgtaaga tgagtaaatc
    41161 tttaggaaac atcattgcac cagaaaaagt gatcaaacaa tttggtgcag atatccttcg
    41221 cttatgggta tctagtgtag attatgaagc agatgttcgt gtttctatgg atatcttaag
    41281 tcaagtggca gaagtatatc gtaaaatcag aaatacgatg cgtttcttat tagcgaatac
    41341 agaagatttc gatcctaaaa aagatactgt ttcgtacaat gatttacgtt ctgtagacaa
    41401 atatatgacc gttcgattga accaagtaat caaagaaatc cgagaagaag ggtacgacaa
    41461 atataacttc atgcatattt accgtacagt tatgaacttc ttgacggttg atctatcgtc
    41521 attctacctg gattttgcta aagatgtagt ttatattgaa gcagaagatg attatcaacg
    41581 tcgttgcatg caaactgttt tctatcaaac agctgtagct ttgacaaaat tattgactcc
    41641 tatcattccg catacagcag aagaaatctg gtcattcctg aaagaagaag aggagtacgt
    41701 tcagttaagc gagtttcctg gttatgaaga gtttgcaaac caagaagaat tgatggatac
    41761 ctggactgct ttcatggact tccgtgataa tgtcttaaaa gcattggaag aagcaagaaa
    41821 cagcaaattg attggtaaat ctttggaagc aaaactaact gtttatccaa aacaacaagt
    41881 tcgtgaaatg ttaaaagctt tggatgcaga tattgcacaa ttattgatcg tttcaccaga
    41941 ctactttgaa gttatggcag cagatgaaca agtgccagat aacgctatgg tatttgatga
    42001 tgttgctatt acagtggaaa aagcagacgg ggaaacttgc gatcgctgtc gccaagttcg
    42061 taaagatgta ggagtagatg aaaaacttcc tcatctatgc ggtcgttgtg caaaaatcgt
    42121 tgaagacttc tatcctgaag ctgtagctga aggattcgaa gaaaagtaaa agggaaaagg
    42181 gggtgtgaca tgttctttgt cacaccccct tttgttatca atcgtttaat tttccacgtt
    42241 cacgatacca gttatctggt tgccagatcc attcgaatcc gtctttttca agtagttcga
    42301 aagctgcttt aggtcccatt gatccagctt tgtagttggg aaaatccggt tgtgtttggt
    42361 cccaagcttt tcgaatgaca tccacgatat gccaagattg agcgacttct tcccaatgag
    42421 agaagttcgt tccatcacca ttcaacgcat ctaatagtaa tttttcgtaa gcttctgggc
    42481 tattttcaac catttctgca ctatttctaa attccaattt gattggttcc gtttcaaatc
    42541 cttgccctac ttctttccca ttcaaggata aagaaaatcc ttcagttggc tgaatataga
    42601 tcgttagaac attaggtgga agtgtcgtat cgtcacatgg ttcatcgaca gatgttttga
    42661 atacattaac aggaacttgt ttgaatacga tgttgatccg agtccctttt tcagtcaaac
    42721 gtttgcctgt acgtacatag aaaggaacac ctgaccaacg gaagttatcg atagtgaatt
    42781 tgcctgcgac gaatgtttca gtctctgatt gagcatccac tttgtcttct tcgcgatagc
    42841 ccttgaattg tacaccatcc aattcacctt gagcatattg cccgcgaacg aagttttcta
    42901 acacttcttc ttcgttataa atgcggatag ctttcaatgc tttgatcttt tctgtacgga
    42961 tttctttttc ggaaaatgct actggcggtt ccattgcaag taatgctaca acctgtaaaa
    43021 tatgattttg caccatgtct ttaagtgcgc cactatgatc gtagtagccc ccgcgatctt
    43081 ctacaccgag gttttcgcca aaagtaattt ggatattgtc gatatagcga ttgttccatt
    43141 gagcctcaaa aatattattg gcaaaacgga tagctgagat gttttggatc atttcttttc
    43201 ctaaataatg atcaatcctg aaaatatctt gttctgggaa tacttctctg atttgctggt
    43261 tcaattcaaa agcagactgg taatcagaac cgaacggttt ttctatgatc aaacgattga
    43321 agccattttt tgtcataata ttttgtgatt tcaaatgaga aacgatcgtt ccaaaaaatt
    43381 gaggtgccat agccaaataa tagacacggt taccgtttaa ttgatagtgt ccatctaatc
    43441 ggtcagacaa ttcttttaat gtctgataat gcgttgaatc atttacatca tgggattgat
    43501 agtaaaaatg gcttgcaaag gtagttgctt cttcatctga aggattcaat gcacggattg
    43561 tttctttgac aacttcacgg tagtgctcat cgctccaggg acggcgagcg gttccgatta
    43621 cagcaaaatg ctcgcctaaa tttccttttc tgtaaaggcg gaacaaagac gggtacaatt
    43681 tacgctgggc taggtcacct gtaccgccga atatcgtaaa caatacattt ttttcattag
    43741 acatgtggat acactccttt atattttatc gacaaaaatc gtgctggccg ccttaaagct
    43801 gacggggatt ttctttctat cattttggat agtgatcggt ccttcataag ctgcgatgtc
    43861 aacgatctca tattcttcgt tgatattcag gtcaatagtg actaagtaat ccaacaattc
    43921 tttttcatca agcactcgtg cgatacgcac ttttgtaccg acaggatagg actccaatgt
    43981 ttgtcggcgt tcttcatgga caacttcctc ttttttagga atcgatcctc catgaggaca
    44041 atgttcagga tgattcaaat agttttctaa atgatctgca agatgttccg aagtcacatg
    44101 ttccaatact tcagcatcat catggacttc attccagcta tagtctaaat gttcaactaa
    44161 aaaaacttcc cataagcgat gtttgcgaat cagactgcta gctttttgca gtccgcttgc
    44221 agtcaattgt acaccttgat aaggggaatg ttctacaagc tcttctttca caagtttaga
    44281 aatcatttca ctgacagatg cagctgagac atctagacta gatacgattt gtttattgtt
    44341 gactttcgtt gtatctccac ctagttctaa aatgattttc aaataatctt ctcgatttgg
    44401 tgtcatattc gatcatcctt tcatggtaaa aaaccatcat tagtaagtgt aacaaaaaaa
    44461 acgttaattg actatttttc taaataaaaa acaaaggatt tttagacgtt tttacaacaa
    44521 aaaacgaaag atgatagaga agactcattc tttcgtttat aaaggatata tatatacatt
    44581 gggagggatc tatttttcag ataacaactt atatgaagtg tctgcagatt cgtcataaag
    44641 agtttgttcg ggataagaaa agataagata aaaaccatac tgatcacgta cgactaaacg
    44701 atattcatct atttctacca cagatgtgcc aagagactga ccgttgatca aaaacgtgta
    44761 atcagatgta atctgtaatt gttgtttttg ctggctatca ctaaagaacc atgtaccttg
    44821 atactttgag agtccttctg acttttccgc ttgttttttc tttttcaagt aaataaaact
    44881 tgagagaacg gtaatgagtg aaccaagaaa atagatcatt ttttttatca ccaaatcact
    44941 cctttatgag aaaataatcg catagacata ttctttcttc tttatttcat tgtaccgaat
    45001 aaaagctaaa atttctctaa gactttcgac tggttttttt actgtttttc tacaaattat
    45061 gataggatac atagtaggaa gaaaggtgac ataatttgaa gtatcagcaa gtggaaatag
    45121 ctttttttat tgaccgcccg aatcggttta tagcacattg tatgaaaaaa gacggagaaa
    45181 taattattgc gcatgtcaaa aatacaggta gaggaaaaga ggtcctgata ccaggcgctg
    45241 aagtggctat tgtatttgct cctggaccta aacggaaaac cgcctatgat ctgattgctg
    45301 taaaaaagaa gggtgactgg tttaatatcg atagtcaatt gcctaatcgg ctggcaattg
    45361 atggtatttt agacggcacg atccttttac caggattaac tggtccattt acggtataca
    45421 agcgtgaagt aacttatctt cactccaaat ttgatattta tttagagacg gtggtcggac
    45481 aaaaagcatt tgttgaagtc aaaggtatga cacttgaaaa taaagcgatt ggtgcttttc
    45541 ctgatgcgcc cacactcaga ggattgaaac atataggaga attaacgtat gccgcacaag
    45601 atggttatgc cgcttatgtc ttgtttattg ctcagttcga acatcttcat ctggcaacga
    45661 tccatgaaga aatgcaacca gcactagctg atatggttcg acatgcgcag caaagcggag
    45721 ttcaaatcct cgcctataat tgtcaagtat cggcagatga ggtaactgtc aagcaggcca
    45781 ttccatttga tgtagatatc ccttttgaag atcctataca atagaaagaa ggtaaaataa
    45841 tgatcaactt aggtattatc ggaacaaatt ggattactca tcaatttgtc caagcagctt
    45901 taagcacaaa aaaatatgaa ttgacagcgg tatattctag acatttggaa aaagcacagg
    45961 aatttggttc tcattatgaa ggggatatcg aattcgcaac agatttgaag acttttttca
    46021 atattactca tatggacacc atttatattg cttctccgaa tagtcttcac tttagtcaag
    46081 ctaagcaagc aattttggca ggaaaaaatg tgatagttga aaagccagct ttttcgacgc
    46141 caaaagaaat ggatgaaatc atccatttgg caaatgaaca caatgtgtat ttttttgaag
    46201 cagcaagaaa tatccatgaa tcaggtttta aaaagattgc cgattactta ccattaaaag
    46261 acgagataat cggtgcaaat ttcagctata tgaaatattc ttcgcgctat gaccaagtat
    46321 tagatggaga agaaccgaat atcttctcga ctcatttctc aggtggtgca ttgatggatt
    46381 taggtgttta ccttgtatat gcagctgttg cttggtttgg aatgccaaaa gaagtccatt
    46441 atttcccaag aaagatttct actggtgtag atggtcttgg atgggggatc ttacgttatg
    46501 agaattttga tgtatcgatc cagccaggta aaatcggtga ttcttattta ccgtcagaga
    46561 tttattttga ctcaggaacg ctagtgatga atggagtcaa tgcaattgaa caagcacaat
    46621 ttcatgatcg cgcgcataaa gaaatcattg atttggcggt aacagctgca gaaaatccaa
    46681 tggcagatga agcagaggat tttgccaatg tcatggctca tccgaaagat ccagcttggg
    46741 gattgttgta tgaagagtgg atcgagctag ctagaaatgt gaaccaagtg atttatgatt
    46801 taagaaaaaa tggcggcatc aaattcgatg cagataatga ggaagatttt taatgaacga
    46861 atttgtacaa gtatttcccg aaaattggca gaaaaaatat gaggataaag ggtttatctc
    46921 accttctctt attcaacgag cagtttttca gccactaagt aataaacaaa gtatagtagc
    46981 ggtttctcct acaggttcag gtaaaacact ggcttatctt tggcctctac tactaaacgt
    47041 ggaaccaggt gaagcaagtg cgttggtcat ttttgcttct tctcaagaac tagctattca
    47101 agtagcagat gttgcacgtg aatggggaaa agacaaagag ttgaaagtcc aatctttggt
    47161 gggcggagcc aacgtcaaac gtcagattga gggtttgaag gaaaaaccag aaatcttgat
    47221 tggtacacca ggaagaatat tggaattgat gaaagcaaaa aaaataaaag cacatcaagt
    47281 gaaaacaatg gtatttgatg aagcagacca gctatttgac gctgggaaca gccaaatcat
    47341 tgatcaaatt cttcatcaag ctccaacaga gtatcagcta gctttttttt cagcgacagc
    47401 agatcgttcc cttgaaagca ttgaaaaaat cactgggaaa acgtttgaga caattgacgt
    47461 gacagcagag gacgattctc gaaaaggact gagacattac tttttacgtg taccgatacg
    47521 aaagaaagat gactacttga gacgtctcac gcatatagaa gattttcatg gtctgatttt
    47581 ctttaatcaa ctgactgaat taggcgtaat ggaagaaaaa ttgctgtata gaggtgtacc
    47641 ggtaggaagt ctggcctctg atcagaacaa gctactccga aaagcagcgc tagaacaatt
    47701 caaaaagaaa atggtcagag cacttttgac cacagatgtg gcggctagag gtttagatat
    47761 tacaggtctt ccttatgtag tgaatgcaga agttccgtta agtgaagaag cttatttgca
    47821 tcgttcggga agaactggtc gaatgggtaa tgaaggaata gtaattacac tcgtacaaga
    47881 taataatcta agagatttaa aacggctttt acgtccatca aatataacac ttgaagagat
    47941 ttttttatac ggaggtaagc tgcaaactga gcagccaacc aaagaagatg cagctcctgc
    48001 agtaaataaa aagtatgcga aaactttttc tggaaaggag cagaacccag aatataacaa
    48061 agaaaaaata agaaaaaaag ttaaaaataa atcaaaaaaa gagagaaata aaggagcaag
    48121 gcgcaaataa gcgccttttt atgctatgat tatgaaaacg ggaacaaaat tggttgccag
    48181 aggaggggtt atgtgaaaga acgtcaagaa gagatcttgc agctattgac agaaaaccaa
    48241 caaggattga cagcaagtga tgtagctgag cgcttaacta tcgatcgaag caatgcaagc
    48301 cgatatttaa gtgaactata caaggcccat catattgtaa aaaccgctgg ccgaccagtc
    48361 gtttactctt tgccaacgga aaagtcaaaa tctgatgaag tgcatgtcga ctcttctacg
    48421 caagtgactt ttgaaacact cgttggagaa aatgattctt taaaagtcag catacagcag
    48481 gcaaaagctg tgattcttta tccaccgcgg gggttgcata cgattatttt tggagaaact
    48541 ggtacaggga agtcgatgtt tgctgaatgt atgtatcatt ttgcaatcga ttcagagatg
    48601 ctttctgcag atgcgccatt tgtttctttc aattgtgcgg attatgctca aaatccgcaa
    48661 ctgctctttg gacacatctt tgggatcaaa aaaggggctt acacaggagc agctcaagat
    48721 agtcccggtc taatagccaa agcagacggc ggtatattgt ttttagatga gatccaccgt
    48781 ttaccgccgg aagggcagga aatgctgttt actttcattg ataaaggtat ttaccgtccg
    48841 cttggagaaa gtgcacaagt acatgaagca tctgttcaga tcattggggc gacgactgaa
    48901 tcttcagaaa gtttcttatc tacgttcaat cgacggattc ctatggcaat caccttgccg
    48961 aatcttgctt cacgttcact ggatgagcgt tatgaaatca tctctctttt tatcaaacaa
    49021 gaagcgaatc gtttgaatca acgtatcgat atcgaaaaag aagcgattct tgccttcatg
    49081 ctttatgatg cggaaggaaa tatcggtcaa gtgaaacgag acttgaaact agtctgtgca
    49141 aaatcttttt tacattaccg tactcatcat gagaaaaagc taatcattcg aaaagaagaa
    49201 ctttcccttc aagtacaaaa aggattactg aaaatcaagg aaataccgga acgtctcgac
    49261 cgattcatgg acagcaaaag tcagtatttg acatttgaac caggatttgc tgatgttgtc
    49321 tggtcacaag atccagaacg aaacatgcag gtctataacg atattgagga aaaagtcact
    49381 tctctttccg aaaccggtgt ggagaacatt gatctagaga ccctcatttc aaaagatgtg
    49441 gatgcttatt tccagacata tgtcaaagaa ttgactaaaa gttcgattcc caaagatctt
    49501 ttgccagatg aaatctggcg gttaaccaat caattgtatg atgtggctga aaaacggtta
    49561 gaccgtattt ataatgaaaa agctcgtttt gcttttgctt tgcatctgca aagtacattg
    49621 gatcgtgtga gagaaggtca tatgatcgtc cacccagatt tgaataacgt acggaaaaat
    49681 tttaagaggg aattccaggt agcaatcgat ctttcaacga tcattgaaga agaagaacaa
    49741 gttgagattc cttttgatga aatcggcttt attagtatgt ttctttctat caatctagga
    49801 gaaagtgagc ctgttaaaga aaacaaagta gatgttctca tattgatgca cggcagacag
    49861 actgcttcaa gcatgttgga gatggcacaa gaactattag gaacaaaagt agggaaagct
    49921 tttaatatgc cactggttat ggaagttcaa acaatgtatg aagaagtgtt acaatatgtc
    49981 aaaaactatc aagagcattt gacgaatggc ctactcttgt taacagatat gggatcatta
    50041 aacaccttca gtgatttgat ttatgaagag actggcattc ggacgaaagc tatcacgatg
    50101 acaagtacca tgattgtgat agaagcgata cggatggcaa gtgttggaag aagcttagaa
    50161 gatatttatc aaaatatcca gctttccttt gaatcgatcg tacgtgatca gttcaaagcg
    50221 gaaaagtctg aaactttaca gcgtaaaaaa gccgtaatcg tgacatgctt tactggtgaa
    50281 ggagtagctg ccaaattata tcagcgtatt atgccagtga ttaatcaaaa taaggtagaa
    50341 ctgatccaga tgcagttttt agaacgtgaa tcattcaaaa aacacatcga tgccttattg
    50401 gaagaatatg agatcaaagc aattgcggga acagttgatg ttgattatca aaatatccca
    50461 tttttttcag cctatgacgt ttttgatgat gaaaaactaa atattttaaa gcgtatagct
    50521 gatgatgaga tcccgacagc taaaattgtc gattcacttg ctggatcatt aagcgatgtc
    50581 ttttctattg atgaactgat ccgttcattg caaaagacag ttcagcagat ccagaccgat
    50641 ttgtcgatta tcgtggaacc tggagtagat actggaatcg ttatccattt agccttcatg
    50701 ttagacggat tgatcaaaca agaaagccat cggcaatttg agaaactgtc tattttctca
    50761 aaaatttatc gtttggaaat ggatatcgtt cgggcaaact tgatggcact agaacgaaaa
    50821 tatcaggtaa aaattccaga agatgatatc gcatttctta cacaaatgtt tcttgaaaat
    50881 aaaataaaaa aatgaacaca ctaatcatac tgtttacaca ctaaacatct gtgtgcgcac
    50941 tataaaaaag agctaaaaag catgaaaacg ctatttcagc tctttttttt attggcatgc
    51001 ttcttgcgtt atataaaagt gtaaaaaaat aaaaggagtg ttaaaaatgg caaaaaaaac
    51061 aatcatgtta gtttgttcag cagggatgag tacgagttta ttggtcacaa aaatgcaaaa
    51121 agcagcagaa gaaaaaggga tggaagccga tatttttgcg gtctctgcct cagatgcaga
    51181 taataattta gaatctaaga atgtcgatgt attattatta ggtcctcaag tacgtttcat
    51241 gaaagctcaa tttgaacaaa aacttgcgcc taaaggtatt ccattagatg tcatcaatat
    51301 gcaagactat ggcatgatga acggagaaaa agttcttgca caagcagaaa atctaatgaa
    51361 ataaatcaag tatgcagttg ggggtataac aacgtggaag atcaacaaaa tctagaagcc
    51421 atcatggggt taatcatgtt tggcggtaat gcaaagagcg atgcaatgga agcgattgcg
    51481 gctgctaaaa tcggtgattt tgagttagcg gataggaaaa tcgctgatgc agaagaatca
    51541 cttatacaag cgcatcatgc acaaacagac atgttgacac aagaagcaca aggaaatcat
    51601 atgcaagtga cattattgac tgttcacagt caagatcact taatgacatc tattgcgttt
    51661 actgatttag caaaagaaat catcgaactt tatcgtcgcg tatacagcga ataaatacaa
    51721 cttctttttt gaacggaaga agaaaaattt ttgaacggat tttttatttt gttataagga
    51781 gaactctctt tatataaaaa acttatagga ggaagtaaaa aatgaacgga ctatcaagtt
    51841 ggatggagaa atacatcctg ccggttgccg caaaaatcgg agcgcaaaaa catttagttg
    51901 ctttgcgtga tgcttttatc ggcatactgc ccgcaacaat ggcgggatca gtcgctgtaa
    51961 tgatcaatgc aattattcgt gatttaccgc aacagtttaa cagtacctat gctgacagtt
    52021 tagcagcaga caaaggaatc tttgccgtat tgaatgtcat catcggaatc aatggttttg
    52081 tctggaacgg tacactagca atcgccggat tgatctttgc cttttcttgg gggtataatc
    52141 ttgcccgagc ttatggagta aatgatctag ctggggggat cgttggttta gctaccttga
    52201 ttcaaggtat ttcctttggt ccagcattat cagcagcatt agatgtgaat gttccttcaa
    52261 atgtcgcaga tgcaatcaat agttctgaga ttggtgcaac gattgcagat ggcacactgt
    52321 cagttggatt atggggttgg ctaaaactag atcatttgaa tggaaacgct tattttacag
    52381 ttatgatcat gggagctatt tcagtcatta ttttttgtaa attgatgctt gccaacatta
    52441 cgatcaaaat gcctgattca gtaccaccag cagtatctaa agcatttgca gccattttac
    52501 cagcgacgat cgctttatac gtcattgcta ttatcaatta tatcgtaggt cgtgtagctg
    52561 atgggcaact gatcattgat ttggtgcaaa aatatattgc agaaccattc ctaggattat
    52621 ctcaagggtt gggagcagta ttgatcgtca cagtatttgt tcaagtattc tggttctttg
    52681 gtattcatgg atcaaatgta ttggcaccag tgctagaggg aatctgggga caagcacaac
    52741 taatcaatat tgatattttc caaaaaggtt ataacggaaa aactggtaca ccagccgtgt
    52801 tagatgcaat cgaagatgga aaagcttata tgtgggtccg cggatcgttc gatgcttttg
    52861 catggtttgg cggatcaggc ggaacgatcg tcttactgat agctatcttg attttctcta
    52921 aacgtgcaga ttatctaaca gtagctaaat tatccattgg gcctggtatc ttcaatatca
    52981 acgaaccagt gatgtttggt ctgccaatcg tattgaatgc aatcatgttt atcccattct
    53041 tgattgctcc agtagtagca acagcaattg gatggacagc tacttatctt ggcctagtcg
    53101 ctccggtatc gcagcaagtc acatgggtag taccgccgtt cttattgtca ttcttagcaa
    53161 caggagcgga ttggcgggca ccgatcgtaa cattagtatg tatggtcgtg actttcttga
    53221 tttgggcacc atttgtcatc tctgcaaaca aaatggaccc tgccgcaggc gaacaacaat
    53281 aagatgaacg tgaggagcgg gacgctttaa gtttcgttcc tcataatgaa ttttcgagga
    53341 ggaactgaaa tgattacaat tgccagtgca atgcaacaag atgaaatcaa agaattgtta
    53401 gaaggataca atgaagaagg aatgactttt acatttaaag aaaaacaagg aatcaagctg
    53461 ttctttgaaa caagtgcaga agatccggaa gttgcggcta aaaaagcaaa ggaactaatc
    53521 aaagcacagc cttggggaac agtcctttat tttcaatcaa cagctgttta gaaaatggtt
    53581 taaaaagata ggatggttga gatgataaaa aacggtttat ttttatgtag tattggttct
    53641 ttgatcgtgt tgtatagttt gaatccacat tcgcaaaggt atgatttgta tagtatggtc
    53701 acaggtcttt ttctaacagg attaggaatc tatttcttca tgaaggggaa aaaaagagaa
    53761 gaagcaaagg agaaaaataa ctaatggaaa tcacgaaaaa attaaatatt ccagcatcgt
    53821 ttttttacga taaagttatg gactctgtcc ttttcgatgt acgtaaagcg accggcaaat
    53881 cagtaactag aaaacagctg aaaaatttgg agtacgtcaa acaattctcg caaaataacc
    53941 gggctcgcat aaaaatcgaa gaagtcattg aaaaccagtc ttataagttt cgtacatcga
    54001 caacgaagaa tgaattcgtc gttcattatc aaatcaaacc attggacgat tcgacctgtg
    54061 aggttcatta cacagaaaaa atggagtctt atggcatgct tcaaaagttg aatgatatgc
    54121 ttttaggaac aatcttgatg tattttaaac ggagacgttt caaaaaaatg ctagatatga
    54181 tcgaagcaac gtattaaatc aatgaaacat ggtactattt agttattgaa aaatgaagta
    54241 agctagaagg aatttttcat aggaagatag atctttcctg cgaatcctca ctagcttttt
    54301 tgtaggaagg ggcagcaaaa tgacaaacaa agtaagagag ctaggtgtat cttcgattga
    54361 ggaaatgttt gatttagcca cttatgcatt taatggggca gatacacctg aaaggcgaga
    54421 acgttttcgt acacttgcgg agaattcttg gaattacgga ttttttgatg aggaggatcg
    54481 gctaacaagt caagtcatgg cgaccccttt tccagtgaat ttttacggac aagagtacct
    54541 aatggccggc attggctatg ttgcttctta tccagaagca aggggacaag gcgggatcaa
    54601 tcagatcatg gaaaagatcc ttgaagattg ccgtgatcgt aaagtaagct tatcttatct
    54661 ggcgccattc tcctatccct tctatcggcg ctatggctat gaacaatcat ttgataaaat
    54721 cagttatcaa ctaaacagtc gtgatgtgcc gtttatcaaa aaaaaatctg gaaaaataaa
    54781 gcgtatggat tttgaagatg caaaagctgc aatcagaaga atttatgaac agatgccgga
    54841 aaaccaacga gctggtctga agcgcgaaga ctggtggtat acgtataaat tcaagcaaaa
    54901 aggcaattac cagtttgctt tgcattttga tgataatgag gaagcaacgg gatatatcgt
    54961 ttatcagctt gcaacgtctt cctttattat tgcggagtgg ggatttttgg atcatgatgc
    55021 tttttgcagc cttgttcgct tcgtaagttc tcacaatggt gcttttgaaa catttgaata
    55081 ttcatgcggc tacggcggta atgatcaaaa ttatctatta gaaaatccat ttgcttctgt
    55141 aatgatcaca ccttatatga tggcgaggat cgtagatatt tctttgtttt tggaaaatta
    55201 tccatttaaa aagagacaag ctgcatttgc cctacaaata gaagaagatc attatgcgaa
    55261 atggaatgaa ggaatcgtgg aagtaacaat cgaaaatgga aaaaatcatc tctcaaaagt
    55321 agaacaaaca aaactcccaa tcgttcgagg aagtatccaa catatcacgc agctattatt
    55381 agggtatcgt aaagtggaag agttggtatt ccaagaaaga atacaggtga caaaagaata
    55441 tgaagaaacg ttaagctcaa tcttgccaca gcaacagcca attcttaatg actatttttg
    55501 ataaaaaacg ggtgatgaca atatttttgt caccaccctt ttgtttttca tttggcattt
    55561 gaaggaaaat gttaaagtaa atcctctttt ttcgtaaatt tgaatttttt gttttctttt
    55621 gttttagggt tttctttttt gtggtcttgg attgcttctt tcgtaatctt ttcccgattt
    55681 tttttagatg aatctaagcg ttctgcgtat actttttctt ttggcttatc attcattgga
    55741 aaggacctcc ctacattatt tcattctacc tctagtttat catctgttaa ggactagtgg
    55801 aaacgttttg cctaacttat aagctgattt ttttagcaga taagcttgca agtaaaaaac
    55861 aaacaagcta caattgattt attgaaattg ttcctgtagc tcagtaggat agagcaatcg
    55921 cctcctaagc gataggtcgc tggttcaatt ccagtcagga acgtataaat aacccctgta
    55981 tcctttgcgg tacaggggtt tgttttgtat ttgtgccaaa aatgtgccaa actagttatc
    56041 atttttatat aaactatcga acacagtatt gattttttct ttatcctgat cttcaagctc
    56101 tcgaacaata tgaagataag tagacattgt agtttctaaa ctgctatgtc ctaaacgttt
    56161 cgatacgcta agtatattta ctccttgata aagtaaaata gatgcatgtg tatgccttag
    56221 tccatgcaaa gtaaattgct tttcaatccc taattctttt aattttttgc gcaaatattt
    56281 gctgacggcg tttgatgaaa ctaatccatt ttttaagttg aaaaaaacaa aattatgagg
    56341 gtttttgatt tcaaaattct cgaataattc tttttgattg attttaaact tttttaagag
    56401 atcaatcgta tgtttatcaa tggaaatttt cctatttgaa gtttcgtttt ttgtttttcc
    56461 ccattcattt aatttataat tccatgtttt attaatagtg atgatttgat cttcgaaatc
    56521 gatatcttcc catgttaatc ctagaagttc agcaaatcgc attccagtgg caccagcaac
    56581 taacacgagc ataggagaag agtactgcgc agaaaggttt tcttctacga gtttcattaa
    56641 agatttaaat tgatcataat ctaaatattt atcttctttt ggcttcaatg aatcttttcc
    56701 tttgattact gcttttctag ttggatcaaa cggaattaat ccttcttcta cggcatcttt
    56761 caacgatgct ctaatatggt tattaaactt aataacggtg gattttacat ggtctttcgc
    56821 atacttattt aaaaagcgtt gatatccaac tctatctaaa tcagaaatca ataccgctgg
    56881 catatatttc tttatgttca ttaacgtatc ttcgtatttt ctataagtga taggagatac
    56941 tgttccttct ttataaagtt gcatccaatc ctcaaagtaa tctgataata atagattttt
    57001 gcgatcccct ttaaggcctt tgttcaagtt atattctaat tcgttggcgg catcttttgc
    57061 ttcgcctttt gtcttaaatc ctgattttct tatcttcgtg tattttccgt catctttttt
    57121 gtatgaaatt tcgtattgcc aactatttcc acgtttgact aatcttgcca taattgaatc
    57181 tactctcttt ctctgataca ataggcacta taaagaagcc tactgtatag gtttgttttt
    57241 cttagaacac gcttgctttg gacggtgggc gtgtttttta tttattatac agttgcttta
    57301 aaaatagctg ctttttcaaa tgatactaaa ggagagaagt ggatttctat ttctcccttt
    57361 tggtttaacg tgaaatgagc agttacatcc atttgttttc ctggagcaac agatcccatt
    57421 gtattttcgt tggcatatgt ctctgatttt ttgtcatctg gtccatatac ttccacatct
    57481 gtacctacag ggatatctga atcgccatca ttttttacgg tataagtaat ttttactact
    57541 tgtgcaggtt ggttttcttc aaattgattt ctttcatcag ttagttctac actatttagt
    57601 gtatattcag catcaccaac ttttacagtg tcaccaatct tatagaaggt atcgcttttt
    57661 tcttctttag aagatgaagc agaggttgaa gatttagtta ctttttcgcc accattatca
    57721 cttgctttat tactatctga tccaccattt aaagcagaac caataattat aattaaaatt
    57781 actgctaata cccaaaacca tacttttttg taaaaaggtt tactaacttt atacattttt
    57841 ccgtcttgac ccataacttt ttttgccatt taaatattcc tcacttcttg ttataatata
    57901 tttgcgatct cagaaatgag gtatgagtcc gtgttgcagc acgggctttt tttactgtgc
    57961 ataagagtat tttttcttga aatatgactg gcaaacatta aaacattctg ttcttaactt
    58021 attattgata gcatagaatt ccataaaatt ttccaatttg aactgagatt catctgttaa
    58081 ttcattctca ataaagatat taagtagaat cataattgca attttatcag cttcagtttc
    58141 gaattttgaa tgaaaagttg tagagttatc gtacagtact gaatattcaa aatgtgaagc
    58201 aatgaaatga ccgagctcgt gggctaaatg aaaagcttca gaactatctt cgtgtagttt
    58261 ttcgtttaaa aatactattc ttggttttgg ataataaaaa cctggttctt ccatttccat
    58321 atagattaac tttaaattat actcactcag catttctttc aactttaaat acatacaacc
    58381 catcactcca actattcatt ttcctctaaa gctttagcaa ttgcaatcgc tttacgcatt
    58441 gtctccttag atatttcttt tccatcaaaa gaaaaaacag tatcgtcttc tgataaatcc
    58501 acatgtttag gggtttctct ttcttctcta cctagaaggt agtctacaga gactccaaag
    58561 taatcagcta accgagctaa ctcaatagaa ttaggagttt gtcttttcca acttcctaaa
    58621 tatccattcg aatacccgaa attcatttcg agctgtctaa tggataatcc tttttctttt
    58681 gctaactcct ttattatttc gtaagtattc attgataaat caacctttct gaatgcttac
    58741 aaaaaaagtt tagataaata atgtaaaaat agttgactta tatacattat taatctatac
    58801 tatgttttgt aaacaagttt aacaactaaa aagacaacaa aaaatactat tgattaataa
    58861 atgccaaccg ccaagaaagc tttttaaatc aaagtttaga tgtcttattt aactatgcat
    58921 ttagtataga ttattaatct aataaagtca acgcagtttt ttaaaaagtt gttaaatttg
    58981 tttacgaata taaaagaaag gagagaagaa tatggaaaaa acagtctcgg caaaaatcga
    59041 agatttgaaa ttagacatac tgaaacaagc aaaagtggcg atggaacacg cggtagataa
    59101 agaagactct gccatggttg cagccatagc agagatttta gctcacgttt agtcatcgtt
    59161 tttacggtct tctggaagct gaccatagat aacagcataa tgaatatcta aataagcttg
    59221 aacaatttct tttggcgaaa ttgattcgcc tttagtagat acttgtgatt cgtgatttag
    59281 ccatgcaaca actaaatccg cagcgatttc aaacggcgtt acactttgtg tcagttgcca
    59341 taaagaattt cacaatatgt atggtcgtgg taataatacc aaagagcaat ttgaggaatt
    59401 tatggaaatt aaaaaataca aaaaatcatt tattaaagga ggctaaacaa tgaacaagga
    59461 aatgagccaa acaatcaagg tacaaaaaat gatagatgat ctaacacatg gaattgatag
    59521 ccaagccgat aaaatcatca aggaattgca aggacaaaaa gttaaggatg caaaaatgct
    59581 tctcaagact atcaactttg aaatgaatcc aacaaaaaga aagctcgccg atgtgttgga
    59641 agaaaagttg gcttccgcta tcaacgagca agaattacta tttgaaaccg atacttttaa
    59701 cagctgatgt tttatagatg acagtttcat ccgaatctat atcatcgccg agagaaaaga
    59761 atgttgcttt tgacaaaaag ccgataagac ctacaagcgg tcttgatgtt gcaagagtag
    59821 ataccccaat atcaccttgc aaggtattca cgtaaatttg agaagaatag aacattccat
    59881 ctttttcttc gatatttgac gcatcatcgt atccaacgac caaagtatcg gcaaatattt
    59941 tgattttttc tccatcaagc atagtaatta caaattctgg tttcataaat tttcacctcc
    60001 ttaacaatta tttcagcctg tcacactgat aaggaaatta taccaaagaa aggaatgaga
    60061 aatatgaata caccacaaat ttttaatttc gagcaaaacg aagttcgaac tattttagta
    60121 aatgatgaac catattttgt aggaaaagat gtagcagacg ttctaggata ttcgaatccg
    60181 caaaaagcta ttcgagatca tgtggacttg gaggataaga cgcagaacga ttcgttcacc
    60241 gtcaatggaa cagcagttgt tttgattaac gagtcaggcc tttacagttt aatcttaaaa
    60301 tcaaaacttc cctctgccaa aaaatttaaa cgttgggtaa cgagtgaagt ccttccaaca
    60361 attagaaaaa caggtagcta ttcaaacgta cctcaaagtt ttgcacaagc attgcgttta
    60421 gcagcagatt tagaagaaaa gaaccaatta ctcgaacaac aaattgccga gtacgaacca
    60481 aagattagct acttagatac gattctttca tcgacagata cggtagctac ttctcaaatt
    60541 gcagctgatt acggaatgtc ggcaattgct ctaaacaaat tgctaaacga gttaggtgtt
    60601 caacataaag ttagcggaca atggatactt taccgaaaac atatgaacca aggatacaca
    60661 aaatcgcaca caagtgagat tccgaaagcc gatggcggca ctaaagttgt aatgaatacc
    60721 aaatggacac agaaagggcg agtgtttatt tacaacttat taatcgcaga gggctattac
    60781 cctcaaatgg atttagagga aattggttag aaaggagttt tagtatgact gacattgcag
    60841 aaatcactca acgagataga gaaaaaatca aagaatatgt cgaaagttcg aagttcttaa
    60901 cttacaccat gcttgctgaa agatttggaa ttagcaaaag ctacttatct ttaattttaa
    60961 acggtaaaaa gacttctgca gaagcaaata gaatcataga ttcgattatt actatgtacg
    61021 aattgtaaag ggaggaacag ctaatgcaat atctagaagc gaaaatccca gttccagaag
    61081 gctatgtaat tatctcccaa gtggattatg aggagttaaa aaaagctgat gatactggta
    61141 gatggatgac gttgccagaa gtgctagaac ggattaacag aaaatatgat tggtttactt
    61201 ctagagtttt aaagaaccca agatatagaa atattatcga tatcgaaaaa aataaaaatg
    61261 gatttgtcta ttatccagtt gaaggaagag acacatatct atttttaaga agtaaaacac
    61321 ttgaattttt agaaacaaat ttttcggaaa tcttaaggag gcaagcggat gggaaaattt
    61381 aatagagcgc tagtattcag cgcaccgcta atcatctacg ctttaggact ttggggaagc
    61441 agacaagcgt tgataggaac gattgtttac atggtttgga tttttatggg gctggatgaa
    61501 gctgagtaca aaacaaaaaa gccagtcggg agggactgac taatgaaaaa aagtttaatg
    61561 actataaacg aaaaacaatt gaaagaaaat tttaatgatc tcatcaaaga atttgtaaaa
    61621 gaaactggag aatttcctaa tcaaattcat ctagttgcgg aggggcatag ccagtatcaa
    61681 gctgtgaaat ttgagatgaa gaaacaaatc tattgaattt agcagtacct ctacttgtaa
    61741 gaacatttat tgtaatgttt ttttcgacta aagattcatc gtatctaaaa accaacgtag
    61801 taatttttga ctcatacggt ggaatggtaa taggtaatga caacggagga aattttgata
    61861 gatcagcacc tttttttaaa gatttaccag aatacgcacg tataccatgt ggaagttcgt
    61921 catacattga ttttaccgtt acagaataat tatcaccaat cattgtaaaa gcatttaaat
    61981 gatcaggaat tccagaaatc gaaaactcaa taattgtaac aggtaaagaa ctgttatttg
    62041 ttaacaatac agaatctata atcctgaatt tattttgatg gtaaacatct ggtttggaat
    62101 cactttctct atctatcaac cattcttcgc attcagcgaa gaaatccaat tttaaattaa
    62161 attttttgtt ttttctatct gtataggaga tataaaaagc aaatgttgaa aatactagag
    62221 acgtaatagg caaaataaaa tctttaatgg ttttaacaat gttattccag tcaaattgtt
    62281 taataaagtc aataagactc aatttatacc accaccattt tttatctaca ttatatcaaa
    62341 gaggagagaa gaaataatgc aagaattagt aattttgaaa aataaagaag ctgtgactac
    62401 gagtttgcaa gtcgcagata gttttgaaaa agaacacaaa aatgttttga gagatattga
    62461 aaagttaaaa gaagatgtgc tcaattttga gcagatgttt gtggaaggta atgaaccaga
    62521 ttcatacggc agaaatcgac gagttttctt cattagtaga gatggttttt tcttgttggc
    62581 tatgggtttt acaggaaaga aagctattta cttcaaacaa aaatacattg aagcattcaa
    62641 cgaaatggaa gatgttattc gtaagaatac tgttcctcaa acaattgaag atatgatgat
    62701 ctaccaacta gaagaaatga aagatgttaa aaaagatgtt tccatgctta aagatactat
    62761 gcgaattagt gggcaacaag agtttgaaat taaacaaaaa ggaaatataa aagttatgga
    62821 agttctaggg ggtaaagaaa gccgagctta tgaagaaatc agcaaaaaag tattctcaaa
    62881 attttggtct gaatttaaac gtaccttttc aatcccaaga tatggcgagt tacctcgtaa
    62941 gagattcgat gatgctgttt catttattga aatgtggtta ccagaaactg cgatccgcat
    63001 ggaaatcgat caactgaaca gacaacaaag acttttcggt gatgaaaatg aatagagctg
    63061 aagcgctaag aatcgggacg gtaattgcta atcgctggtg gagacacaat aaaccaatca
    63121 tcctaagcca acaacatatt gacaagcaaa aagcatggca acaaataaaa aagtgactcc
    63181 gccggcaagc aaagagtcac aaagaaaaca catcataagg agattttagc atatg
//
LOCUS       DBNICI010000003        49996 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.003, whole genome shotgun sequence.
ACCESSION   DBNICI010000003 DBNICI010000000
VERSION     DBNICI010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 49996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.003"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..1008)
                     /locus_tag="AC1ZCC_000147"
     CDS             complement(<1..1008)
                     /locus_tag="AC1ZCC_000147"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIQ1628437.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1188..1532)
                     /locus_tag="AC1ZCC_000148"
     CDS             complement(1188..1532)
                     /locus_tag="AC1ZCC_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HIQ1628438.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(1525..1959)
                     /locus_tag="AC1ZCC_000149"
     CDS             complement(1525..1959)
                     /locus_tag="AC1ZCC_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIQ1628439.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            2097..2834
                     /locus_tag="AC1ZCC_000150"
     CDS             2097..2834
                     /locus_tag="AC1ZCC_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1628440.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            2827..4038
                     /locus_tag="AC1ZCC_000151"
     CDS             2827..4038
                     /locus_tag="AC1ZCC_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1628441.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            4242..5024
                     /locus_tag="AC1ZCC_000152"
     CDS             4242..5024
                     /locus_tag="AC1ZCC_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIQ1628442.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            5094..5756
                     /gene="trmB"
                     /locus_tag="AC1ZCC_000153"
     CDS             5094..5756
                     /gene="trmB"
                     /locus_tag="AC1ZCC_000153"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HIQ1628443.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(5854..7608)
                     /gene="ade"
                     /locus_tag="AC1ZCC_000154"
     CDS             complement(5854..7608)
                     /gene="ade"
                     /locus_tag="AC1ZCC_000154"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HIQ1628444.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            7740..8669
                     /gene="comGA"
                     /locus_tag="AC1ZCC_000155"
     CDS             7740..8669
                     /gene="comGA"
                     /locus_tag="AC1ZCC_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HIQ1628445.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            8626..9675
                     /gene="comGB"
                     /locus_tag="AC1ZCC_000156"
     CDS             8626..9675
                     /gene="comGB"
                     /locus_tag="AC1ZCC_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HIQ1628446.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            9672..9950
                     /gene="comGC"
                     /locus_tag="AC1ZCC_000157"
     CDS             9672..9950
                     /gene="comGC"
                     /locus_tag="AC1ZCC_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HIQ1628447.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            9925..10395
                     /gene="comGD"
                     /locus_tag="AC1ZCC_000158"
     CDS             9925..10395
                     /gene="comGD"
                     /locus_tag="AC1ZCC_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HIQ1628448.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            10367..10714
                     /locus_tag="AC1ZCC_000159"
     CDS             10367..10714
                     /locus_tag="AC1ZCC_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628449.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            10677..11102
                     /gene="comGF"
                     /locus_tag="AC1ZCC_000160"
     CDS             10677..11102
                     /gene="comGF"
                     /locus_tag="AC1ZCC_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HIQ1628450.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            11074..11433
                     /gene="comGG"
                     /locus_tag="AC1ZCC_000161"
     CDS             11074..11433
                     /gene="comGG"
                     /locus_tag="AC1ZCC_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HIQ1628451.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            11605..12612
                     /locus_tag="AC1ZCC_000162"
     CDS             11605..12612
                     /locus_tag="AC1ZCC_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIQ1628452.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            12637..13821
                     /locus_tag="AC1ZCC_000163"
     CDS             12637..13821
                     /locus_tag="AC1ZCC_000163"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016774 - phosphotransferase activity,
                     carboxyl group as acceptor [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HIQ1628453.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14258..15841)
                     /gene="abc-f"
                     /locus_tag="AC1ZCC_000164"
     CDS             complement(14258..15841)
                     /gene="abc-f"
                     /locus_tag="AC1ZCC_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HIQ1628454.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(15946..16074)
                     /locus_tag="AC1ZCC_000165"
     CDS             complement(15946..16074)
                     /locus_tag="AC1ZCC_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628455.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(16419..16562)
                     /locus_tag="AC1ZCC_000166"
     CDS             complement(16419..16562)
                     /locus_tag="AC1ZCC_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HIQ1628456.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(16785..17264)
                     /locus_tag="AC1ZCC_000167"
     CDS             complement(16785..17264)
                     /locus_tag="AC1ZCC_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIQ1628457.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(17337..17621)
                     /locus_tag="AC1ZCC_000168"
     CDS             complement(17337..17621)
                     /locus_tag="AC1ZCC_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628458.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(17699..17893)
                     /gene="ssrS"
                     /locus_tag="AC1ZCC_000169"
     ncRNA           complement(17699..17893)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="AC1ZCC_000169"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(17965..19251)
                     /locus_tag="AC1ZCC_000170"
     CDS             complement(17965..19251)
                     /locus_tag="AC1ZCC_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIQ1628459.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            19389..20060
                     /locus_tag="AC1ZCC_000171"
     CDS             19389..20060
                     /locus_tag="AC1ZCC_000171"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HIQ1628460.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            20026..20514
                     /locus_tag="AC1ZCC_000172"
     CDS             20026..20514
                     /locus_tag="AC1ZCC_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HIQ1628461.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            20527..21474
                     /gene="prmA"
                     /locus_tag="AC1ZCC_000173"
     CDS             20527..21474
                     /gene="prmA"
                     /locus_tag="AC1ZCC_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIQ1628462.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            21475..22239
                     /locus_tag="AC1ZCC_000174"
     CDS             21475..22239
                     /locus_tag="AC1ZCC_000174"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HIQ1628463.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            22472..24685
                     /locus_tag="AC1ZCC_000175"
     CDS             22472..24685
                     /locus_tag="AC1ZCC_000175"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIQ1628464.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            24696..25142
                     /gene="dtd"
                     /locus_tag="AC1ZCC_000176"
     CDS             24696..25142
                     /gene="dtd"
                     /locus_tag="AC1ZCC_000176"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIQ1628465.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            25313..26572
                     /locus_tag="AC1ZCC_000177"
     CDS             25313..26572
                     /locus_tag="AC1ZCC_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIQ1628466.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(26661..27608)
                     /locus_tag="AC1ZCC_000178"
     CDS             complement(26661..27608)
                     /locus_tag="AC1ZCC_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIQ1628467.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            28002..29294
                     /gene="hisS"
                     /locus_tag="AC1ZCC_000179"
     CDS             28002..29294
                     /gene="hisS"
                     /locus_tag="AC1ZCC_000179"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIQ1628468.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            29309..31078
                     /gene="aspS"
                     /locus_tag="AC1ZCC_000180"
     CDS             29309..31078
                     /gene="aspS"
                     /locus_tag="AC1ZCC_000180"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIQ1628469.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            31187..32062
                     /locus_tag="AC1ZCC_000181"
     CDS             31187..32062
                     /locus_tag="AC1ZCC_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIQ1628470.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            32328..34211
                     /locus_tag="AC1ZCC_000182"
     CDS             32328..34211
                     /locus_tag="AC1ZCC_000182"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIQ1628471.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(34286..34570)
                     /locus_tag="AC1ZCC_000183"
     CDS             complement(34286..34570)
                     /locus_tag="AC1ZCC_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIQ1628472.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(34560..35294)
                     /locus_tag="AC1ZCC_000184"
     CDS             complement(34560..35294)
                     /locus_tag="AC1ZCC_000184"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HIQ1628473.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            35423..36046
                     /locus_tag="AC1ZCC_000185"
     CDS             35423..36046
                     /locus_tag="AC1ZCC_000185"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIQ1628474.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            36238..37359
                     /locus_tag="AC1ZCC_000186"
     CDS             36238..37359
                     /locus_tag="AC1ZCC_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0004529 - DNA exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HIQ1628475.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            37366..40494
                     /locus_tag="AC1ZCC_000187"
     CDS             37366..40494
                     /locus_tag="AC1ZCC_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIQ1628476.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            40513..43707
                     /locus_tag="AC1ZCC_000188"
     CDS             40513..43707
                     /locus_tag="AC1ZCC_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HIQ1628477.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(43853..44560)
                     /locus_tag="AC1ZCC_000189"
     CDS             complement(43853..44560)
                     /locus_tag="AC1ZCC_000189"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIQ1628478.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            45325..45723
                     /gene="spxA"
                     /locus_tag="AC1ZCC_000190"
     CDS             45325..45723
                     /gene="spxA"
                     /locus_tag="AC1ZCC_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HIQ1628479.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            46055..46708
                     /locus_tag="AC1ZCC_000191"
     CDS             46055..46708
                     /locus_tag="AC1ZCC_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HIQ1628480.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            46934..47932
                     /locus_tag="AC1ZCC_000192"
     CDS             46934..47932
                     /locus_tag="AC1ZCC_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HIQ1628481.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            48108..49916
                     /gene="pepF"
                     /locus_tag="AC1ZCC_000193"
     CDS             48108..49916
                     /gene="pepF"
                     /locus_tag="AC1ZCC_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HIQ1628482.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        16175 a         8741 c        10455 g        14625 t
ORIGIN      
        1 ttttgatgaa tcagttgttt cttcagttga agattctttt gttgaatcag ttgattttgt
       61 gtcagaactc tttgttgaag ctgtagtttc acttgagtct ttcgtacttg atgaagttgt
      121 tgtgaatgca ctcaacacat tttcaaaggc atcatctttg atttttacat ttgcatcttt
      181 taattcttca ccgattactt tagttgtgaa tgcattgtca cttagtttag tttcagtagc
      241 gatatctttt agttgatctt tgtatttatc catgtcattg cctttatttt ggtttttcac
      301 cattttgaca acgtagtatt ctgtcgcata agaagtagga tttgttgtag taataacatc
      361 ggagatctca ccgtctttca atttgaaagc tgcttctttt acttctgcag gaatagttgt
      421 tgtcgttgag tcgaatttaa ctttaccgcc atcttctttt gtctctgtat cagttgattt
      481 gtctttggct aatttagaga aatcatctcc atcatccgct gattttttca catcttttgc
      541 ttcatcttca cttgataatt tgatgatttg tgcttctact tctggatgga aagatttcca
      601 agcagttttc aaatcgtcat ctgtaatatc tacgtgagct ttcaagccag cttcaaaagc
      661 taaattgtta cgaatatatt ctttataagt atctttcgta tatccagcag cttctaattg
      721 tgattcaaaa gtatcaccta atgatttcgc ttgtttgtcg tattctgcat ccacttgctt
      781 atcggttact ttatcgccat atttgttatt gaatacttta taaatgatca tccgttgtac
      841 taaggattgg ttcgatgatt caagttttgc ttcatcatag aaatcagata cagtgatcgt
      901 tccgcctttc atcgttgcga tatctttatt agtatcgcct gaacaagctg ctagagttaa
      961 aacagccaac gcactagtag ctgccaaaat aattgatttt tttttcatta aaatttgcac
     1021 tcctgtaatt agttattatt tttatatatt aactatacca tatcccattt cctttaaaaa
     1081 cgttgtcgtg gtagtttcaa acaaaattca aaatttcatc acattcctag gaaatacgca
     1141 aacttttctc acttatatag ttaaatctgt tgagaaaata atcattatta tatttgttca
     1201 ggatactctg gcagatcagc ttttagtttt tctacttgtt cattgatttg tgtgatacgt
     1261 ggttcggctt ggaatttaaa atcttcgata tcttttttga ttgcttcttg gaaagcagga
     1321 atcacagatt cagctgtttc ttttgtttcg atcaatgcgc ttttaaaatg attcaggctg
     1381 ttatttaaat catttgtcag agttgttgct tcttccaatt cttcgatcag tttctttctt
     1441 gtttcattcc cgctcctggg cgccaataac agacctccga cagtgccagc agcagctcca
     1501 aataacagtc ctttgaaaaa gtttttaatc atcgcaggac acctgcttta cgattgtttc
     1561 ttgaatagct tttagttctt ccgatccata actatcttga tgattgacaa agtgaataga
     1621 aaaatcatcc tctttgctgt agcgagggac taaatggata tgtgaatgaa aaacagattg
     1681 ataggctagt tcacggttgt tattcaagat attaagacct ttcatttctg gaaaagcttt
     1741 ttccaatgct tgagcaatct taggcacacg cgaaaaaacc tctcctgcaa gttcgggatc
     1801 atattcaaaa atatctgcta catgacgttt aggcacaact aatgtgtgtc ctttcgttgc
     1861 ttgagagatg tctaaaaaag catagacctt gtcgtcttca tatattttat agcttgggat
     1921 ttcttgattg atgattttgc aaaaaataca gttttccata taaaacatcc tttctataaa
     1981 gaataaatac ttcagcttaa ctttaccata agttaaagct attgtaagga aaaaaacaaa
     2041 ataccgtcat tttcttcgtg gtataataaa taaaaataaa tggcggagga aaaagaatga
     2101 gtttaattat cgatcatgtc acaggggggt acggacatct ccctgtgtta aaagatatca
     2161 gtttcgatgt acagcctgga gagatagtag ggttgattgg actgaacgga gcagggaaaa
     2221 gtacgaccat caagcacatt atcggtctgc tggaagcttc aaaaggaacg atagaaatca
     2281 atcatctatc cttaaagaaa aatacggaag aatatcgcaa acaaattggg tatattccag
     2341 agactccttc tttatatgaa gaattgacat tgagagaaca tatcgaagtc actgcgatgg
     2401 cctacgatat acctatggat atagcaatga aaagagcaga aaagttgttg gagacttttc
     2461 ggttatctaa tcgtcttgac tggttcccag ctaatttttc aaagggaatg aaacagaaag
     2521 tgatgatctt atgcgccttt ttgatcgagc cttctttata tatcatcgac gaaccatttt
     2581 taggcttgga tccactagct atcaatgcat tgcttgaatt gatggtagaa atgaaaaaag
     2641 aaggagccgg tattttgatg tctacgcata ttttagctac tgcagaaaaa tattgtgatc
     2701 gttttgtggt cttacataac ggagagatac gagcagacgg ttctcttgag atgttgagaa
     2761 aagaatttca gctgccggat tcttctttgg acgaaatcta tattgcttta acaaaggagc
     2821 aggcacatga gtagtttttt tcaaaaacgt ttagcaagac atcaaaagaa aatgatgaaa
     2881 tatatgcgtt atgtattgaa cgatcatttt gttttagttt gtcttttctt gattggcggt
     2941 gtgggattat attattctaa ttggttgaaa accctgcagc caccgtttgc aattggaggg
     3001 ctcataatct ctgtattttg gatgctctgt ttattcgtgg gtaagatggc tacttttgca
     3061 gaggcagcag atattgtttt tttacttcct aaagaaaaag aaatgcaaac ctatttggaa
     3121 cgagcttttc gctattcttg catttttccg ttgatctttt tgatattggc atgcggattg
     3181 gccatgccat tagtggtggt tagtacggag caatcctttt cgatgttttt cgtctatctg
     3241 atcatattat ggagtttgaa attcagtcat ctgcaaatca agagagcaga attgtttcga
     3301 ctagatcaac aagttattcg ttattggaaa ttaggctggt ttttttcttc tctgatcttt
     3361 ttgtttctat cattttattg gctaatatgg gcaggaatag caggtgcatt agtacaggca
     3421 agtctatatt actggttttt gtggaagcaa caagaaaaag gattggattg ggagaaaatg
     3481 gtgcatgtag aagaacatag gttgcatcag atctatcaat ttatcaattt attcacagat
     3541 gttcctgaaa ttacggcaaa gatcaaaaga agacgctatt tggatcctat attgactcat
     3601 attaaaagag aatcaaaaaa cacttatctt tacttgtttg caagaagatt ggcaagaggt
     3661 tctgatttta gtggtcttta tttccgatta gtaattatag gtgggttatt gattgccgga
     3721 gtagtcgact tttattttag tctgattatc ggtttcttat ttttatattt gatcggtttc
     3781 caactgttac ctttagcgaa ccagttccgc tatatggttc tcgttcagct ttatccaatc
     3841 cataagaatc agaagaagca ggcgattcag tatctactca catgggtatt aattatcgcg
     3901 tcggttattt ttggtttgat tgctgcagtc gtattagatg ggcagcagcg atgggttccg
     3961 ctagcggctt atttgattgt tactatgctc tttgttaaac tatatgtacc aactcgtttg
     4021 aagaagatgt cagactgaaa acaaagctgt gtagggtaaa aatgagaatg aaaaatagag
     4081 cgttgacagt aagaatgctg ttcgttctat tttttttgag aaaagctggt tttttgaatg
     4141 gcaattaaat gaaaaagaac gtatatgtgc ttgacgtgta gtgttcaggc gactagaata
     4201 ataagtactg caaaacaaag agtaagagga cgggagaatg catgatggag tttcagttag
     4261 accaagagtg gcggttacgt cccatcaaag gcgacacagg aaaaacatac ataggcatgc
     4321 gtgatgagga caaagtcttt attaaacgaa acacgactcc gatgttggct gctctttcaa
     4381 gggaaggaat tacaccaaaa cttgtttgga caaaacgaac ggggaatggc gatactttga
     4441 cagcacaaga gtggctggaa ggtcgcttgc tgactcctaa tgagattggt caaagaaacg
     4501 acgtgattga tgttttgtac catttacatc attcaaattc tcttaaaacc atgctgaccc
     4561 gtatcggtgg tcgtgtcatg acgccggaga agatgctgca aaaatatgaa aaagaattac
     4621 cggaagaact ccagaaaaat caatttctgc aaattgtcca tcgttttctt cgtgagcaga
     4681 tcccgtcaat agataaggaa aacttatcag tagtacacgg agatgtaaac catcgtaatt
     4741 ggttagtatg taaaaattat ttatatctag tcgattggga ttcaatcatg tttgcagacc
     4801 ctgcggtgga tattgcaacg attttaggaa attatgtgcc actgtctagc tggaatcagt
     4861 ggctgatcag ttatggcata cgcccgactg acgaaatgct tgaaaaattt cattggtatg
     4921 cggccatgaa tttgctccaa gaaatcacca gatattgcaa acgtgaagat catcgacgga
     4981 tgaacgaaga aattttacaa ttaaaacgaa ttttcagtgg ctgaggtttg gcccaaactc
     5041 tgcggagatt tggccagacc tttttaatta gaggaaagga atggagataa ataatgcgtg
     5101 tccgaaatcg tccgggagca gcggaaatgt tagcggctca cccgaatttt gtaattagtg
     5161 atccaacatt gtggaaggga aaatggaatg aattgtttga aaacgatcat ccaatccaca
     5221 ttgagattgg tatggggaaa ggccagttca tcactggaat ggcaaaagcc catcctgaaa
     5281 tcaattatat tggagtagaa atgcaggtca gtgttgtttc gattgcacta gacaagttga
     5341 tcgaacaacc gcttcctaac ctgaaacttc tacatgtgga tggatcagcg ttgacagagt
     5401 atttcgctga cagcgaagta gatcaaatct atctgaattt ttccgatcca tggccaaaaa
     5461 aacgtcatga aaaacggcga ttgacatata agacttttct tgctgtagat gaacagattc
     5521 ttcgtccaaa tggagaaatc catttcaaaa cagataatca aggattgttt gagtattctc
     5581 tggcaagttt ttctcaatat ggcatgatcc tcaaacaggt atggttagat ttacatcaaa
     5641 gtcaatttga aggcaatatt atgacggagt atgaagaaaa attttcttca aaaggtcaac
     5701 gtatttatcg agtagaagct caatttcaag ataagaccaa aaagaaaagt ctgtgaaact
     5761 aactttctgc tttcataaaa acattaatat ccataaataa atcccagaaa atgaacatta
     5821 cagatgtaat gtattttctg ggatttattt gatttaaaat gtatttattt catcggtttt
     5881 tccacaaggt ggaatcaatt ggaagcgaga aaatgaaaat aatcctttgt cggtgatctt
     5941 caattcgggt atgacagata atgttaagaa agaaagtgtc aaaaaaggat caaaggtttt
     6001 agctgctcct agttgatgtg ctttagttat taattcattc aactgttcat agacgatcga
     6061 aaaaggttcc tgtgtcatga ttccgcctat aggaagagaa acacaagcta tttcctgctg
     6121 attgttgaca acggtcatcc cgccaccttt ttgaatcaaa agattagcag catacagcat
     6181 atcctcgtcg tttgtcccaa cgacaactag attatgcgag tcatgggcaa tcgttgtcgc
     6241 tatcgctcct tgtttcagct gaaatccttt cactatgcct aatccaatgt ttcccgtttg
     6301 atggtgacgc tcgatcaccg caatctttaa ttggtctttt tcgattgaag gaatgaatcg
     6361 ttcgccttgt cggtccactt tttctaccaa atctttagtc actagactat tcgggataat
     6421 ttcgatcaca tgggcttgtg aagaagaaag aagcagctct aacgaatgtg gatttaatgg
     6481 ttgagcgttt agttttggca gacaacacgt atatgaattt gaatgatcag caaataaatg
     6541 gtcttggagt tgcccagatt gaacaactaa tgttccctta tagaaaactt catgaattgg
     6601 cagcttggta agatcgtctg ttaagaagaa atcagcttga tacccagggg caatcgcacc
     6661 tagtgttttc aaatcaaaac attctgctgc attcaatgtt gccatctgaa tggcttgtaa
     6721 tggatcgatt ccaagtgcaa tagcttgacg gacgatatga tcgattgatc cttcttttac
     6781 taaatcattg atcagtttat catcggtgac aaagaagcat cttcttgaat tttcaggtgt
     6841 gatcgccggt agtagcgatg ccagatcttt tgcaacggtc ccttctcgaa ccattaaata
     6901 cataccaaga tccaaacgtt ctttagcttc ttttgcagtt gtagcttcat gatccgtccg
     6961 aatacctgct gcaggataaa catttaactc ttccatgccg ataccagctg catgcccgtc
     7021 aatttttttc ttatgctggt gcatcaattg gatcttttct aggatgtctg tttcattatt
     7081 tgctactgct tgatagttca tgacttcagc taagcctaaa acttcgggtt tttgtaaaaa
     7141 aggggctaat ttttttgcat ttaaatttgc accattggtc tcaaaagggg ttacaggtac
     7201 acaagaaggt agcataaaaa agatattcat gagtgtttgt cttgcatctt ctatcataaa
     7261 ctcaagacct gtactcccag caatgtttcc tatttcatgg ggatctgtca ctacagtagt
     7321 aactccatga agcaaggata cttttgaaaa ttcgcttggt gataaaagtg agctttcgat
     7381 atgcacatgg ccatcgatga atcctggcac tagatattgc ccattagcat tatgtacgct
     7441 ttttccttcg tattcaccga ttcctacgat cattccttcg cagattgcta catctttttc
     7501 catgatttct ttcgtaaaga cgttgacgat cttagcatgc ttgatcacta ggtcagcagg
     7561 ctttttttta cttgctactt cgatatattt ccttaatgtt gtttccaatg gagtgcctcc
     7621 caagataaat ttttataagt ataacataaa ttcggttttt gccaatattt ttttgtaaat
     7681 tcattttatt tgaaacaaaa agatcaaaaa gacgtatgtt ttttgtggag gggagaataa
     7741 tggaaataaa acgattatcg gatcaaatga taagtgaaag ttttgccaaa catgcccaag
     7801 acgtatatat attgccagct tcagaagtat tttacatcta ttttcgctat ggaaaaaaac
     7861 gaacattctg gaagaaagtc tcttcggcag atggacagca actgatctca agatttaagt
     7921 atttgggaca gatggatatc ggagaaaaaa gaaaagctca gttaggtgca tttacttatt
     7981 ctttagaaaa acaggaagtt cgtctgcggt tgtcttctgt aggagattac cttcaacgag
     8041 agagtttagt gattcggcta ttatacggta cttttgaaac accccatcgt tgctttagtt
     8101 ctcgagattt tgcaactatt ttacagaaaa ccgtcagacg tggattatac cttttcagcg
     8161 gaccagtcgg atcaggcaag acttctctaa tgtatcggtt ggctttggaa gaaaaattgc
     8221 aagtgattac tatcgaagat cctgtagaga tagaagaacc gttttttctg cagctacaga
     8281 tcaatccgaa aatagcacag acttatgata cacttttgag actcgcatta cggcatcgtc
     8341 cagacttgct aatcattggc gagataagag atgaatcaac tgcacatgct gcggtaagag
     8401 cagcactaac agggcaccgg gtttttgcaa ccgtacatgc acgcaatctt gaaggtacat
     8461 tggtcagaat ccatgaactg acaaagaaaa aagaagagct atctgaatgt ttagctggtg
     8521 tcatttatca agaacttttg gaagatgcca atcaccatcc ttctgtttta tggagttatc
     8581 aatttatgga ggaagaatca gtaaaaaaat catgggagga aagttatgaa gaagctcagg
     8641 aaaatatttg gcagtcagaa ctattctaaa ggattaacaa gaagccagca aaaccagttt
     8701 gcacaaacca tgggagaact gcttttatct ggttttagtc tgcaagaatc cctttattta
     8761 ttactcacga tccgttcgtt tccacctgtt attttggcaa atatccaaca tcagctaaag
     8821 gaagggcaag cgtttagtga ggtactttcg caaataggat atggagcaga gcaactggta
     8881 cagatcgagt tagctgaacg tcatgggaat ctgccgacaa ctttgttgga aattgccaaa
     8941 cagtttcggc ttgttgaagg atttcgcaat gatttgaaaa agctgcttgc atatccgatg
     9001 ctattgctca cctttttgct aggtatcttg tttagtatgc ggttgttcgt tttgcctcag
     9061 cttattggtt caggaatggt catgcaagag cattggggta ttcagctgat acaagtcttt
     9121 cattggtatg ttctactatt catcctagcg gttactctat tggctttact agtaaggtat
     9181 cgtctgatga agttgtctcc tgttgatcag agtgaatgga taagcagaca attattgatt
     9241 ggaccgatat attccttgta tcaatcggct tatttgtcac tagagattgg aaagcttttt
     9301 catgaaggtt tcgaattaag gcaaatcata gtctgcttaa aggaaacgaa acaagaaagt
     9361 ctgattcagc atcttgcctc taagttgatt caaggattgg aggcaggtat gtcattggct
     9421 gagcagttcc agaattatcg ttttctatca gaagaattca gtaaaattgt tttacaggga
     9481 gaggcaaagg gaagtttagg taaagaactg attttttaca gcgaatggac aagaaaacag
     9541 cttttcaaac gtcttcatca ctggatgcat tttattcagc caatcatttt tttgggcgta
     9601 gcggctttga ttttattagt ttatgccgct gttttattac ctgtttacgg aagcatagag
     9661 gaggttttgc catgaaaaag agaaaaatat cttattatag cggttttaca ttgatcgaaa
     9721 tgctaatcgt cttactgatc atcagtgtat tagtgttgtt gttcgtccct aatttgtcgc
     9781 gttataggaa tcatgtagac caagaaagtc gtgaagcgat catccagttg gtagacacac
     9841 aaaaagagct ctatgcgctt caaaataatg gaagagttcc tactgtggag gaattgttga
     9901 atgaaggcta tatcaaacga gaacatgcag aaatctatca gcggccttga aggtaaggtt
     9961 ttagcatcag gatatacgtt gtttgaatca ctcttactga ttttattact tacaacgatc
    10021 ctttcttttc cagttctcgc attttctgca tggaagaatg aattggcagt ctatcaattt
    10081 ttcggccaat ttgaaaaacg aatctattct acgcaaaaaa tcgctattgt gaatcagatc
    10141 caaacaggct tttattggaa tagtgaagag aatcaaatca tttttcatgt tccgaatcca
    10201 gataaaataa attgggaagt attagatatt cctgatgaga ttaccttgaa acgccatgct
    10261 tctattactt ttgctgctag aaccgggaat gaaagttcac taaaagctta tcaattttat
    10321 tgggaagaaa aagagcggac gattacgtat caattccaga tagggagtgg tcgctataca
    10381 aaaagaattg aataaaacag gatttgtttt gttggagtcc cttacagcgc ttatgatcgc
    10441 ttctgtctgt gtctacttag tgagctttgg ccagctgttt ttgcttcagg cagaaaagaa
    10501 aagtggggag gaactgctat ttattcgaat gatttatgaa gatatcaaga cgcagcggat
    10561 gtatcacaaa agaccagagc gaagggaaga aatgatccca tacgatatcc aaattgtcga
    10621 taataatgga gtaatcgaaa aagtgtttgt caatatggag aaagaagcga tcagtattga
    10681 aaaaacaagt aattccagca tttacgctga ttgaatgtct aattggactg atgatattga
    10741 gcagtctatt gctcacattc aatcttttga ttcagcaaac catccaagta agtgaatttt
    10801 tgcagcgtaa agatcaaaaa gagtggctaa tatttttagc acaattagaa gaagaactca
    10861 agagcagtca caatgtctct gtgaaaaatc agcaactgca ctacacgatc gaggataaac
    10921 agtatatcat cgaaagatat caggcaatga tacgtaaaag aagaacttcc ggcggacatc
    10981 aacctatgtt aacaagtatc actgagcttc aattattgga aaaagaacac acaatttctt
    11041 tttatgtaca ctttgaaaat ggagaagatg gatatgccat atggacaaaa aacgatcagt
    11101 agaaaatgtt ataagggctc tattttattt tctgtcttgc ttttatttct tttatgtacg
    11161 tttttattga tggcagcatt agaaagctac cgtttgtcaa tggatcttaa tatacgaatc
    11221 aaaaattatt atatagcaaa aataatgaag cagatggctt tatcggaaca gtctacgctt
    11281 gccgaaagtt cggaaggagt tttttactac acaactggat ctgtaacata tcagtgggta
    11341 caacagtccc tattcttaga ggtggaagtc tcaccgttta tctttcggtt cacagaagaa
    11401 gtaaaaaatg atacagatac tagtacagaa tgatggaatg ttcgtgaaaa cgccctcttt
    11461 tctgttataa cactgttttt aataaaaaaa tgtttgccgt ttcacaaata aaaaacagtg
    11521 aaaacgattg aaattttgtc aattgttcga tagaattaaa caggtgaaaa aattgttata
    11581 atatatctag aaatgaggtg caccatgtta ccagaaaaaa tggaacaagg gtttaatcaa
    11641 aacctggaag ctattcagct tttacagaat gcattgggaa cttccttttt agaagcctat
    11701 gtcgaaaatg cagaaaattt aatagatgat tatcaagtac gtgtggtgga tggagtgccg
    11761 acaaaagaga caacgcaacg gatcacagct ctttatgaag agttgaagaa actttctttc
    11821 gagccagaag aatggcgcag actgtctcaa ttacttttat tgaagggcag ccagacagaa
    11881 catttgcagg cgaaccatca actcactcct gatagtatcg gttttttatt tgtttttctt
    11941 attgaacaac tatacacaaa taaaaaagca ccggttaaga ttctggatat tgcggcagga
    12001 atgggcaact tgttattaac agttctttta aacctatcga atgcggggta ccaaacagaa
    12061 ggaattggtg tggatattga tgatacattg ttggctgtcg cagcttctac aagtgatttg
    12121 acacaagcaa atgttcaata ctttcatcaa gacgggttgc aggaattatt aatagatcct
    12181 gtcgattttg caatcagcga tttgccaatc ggttattatc cgaatgacga aaaagcaaaa
    12241 gagtttttaa caagtaccga agaaggccat agttatgcac atcatttatt gctagagcaa
    12301 tcaatgaaat atgtgaagcc ggatggtttt ggtttgtttt tgatgccgag tggattttta
    12361 gaaacggatc aaagtgaaga aatcaaaaaa tggtttaaag aagaaggtta tcttcaagga
    12421 atgattcaat tgccagatga attgttccgt aataaacaat cgcaaaaaag tattttgatc
    12481 ttacagaaaa aaggaccaca agcacaacaa gtaaaagaag tcttgcttgt gaaacttgct
    12541 tcattaaaag aaccagagaa ggtgacggaa tttttcaacg agtttaaaaa ctggaaatca
    12601 tctgctctgt aatcattcaa cgagaaggag agtcatatgt ctaaaacaat tgcaatcaat
    12661 gctgggagtt caagtttgaa atggcaatta tatcaaatgc cagaagaaac cgtagttgcg
    12721 aaagggatcg tcgaacgtat cggtttacaa gattctattt ttaccatcaa gtacggcgat
    12781 aatcaaaaat tcgaacaaat catggatatc gaaaaccacg aagtagcagt aaaaatgctg
    12841 ttagatcagt tgattgaact gcaaatttta agctcttatg atgaaatcac cggagtaggg
    12901 caccgtgttg ttcacggagg agaaacttat ggggattcag tagtgatcga tgagaaagta
    12961 atggaccgca ttgctgaatt agcagaattt gctcctttgc ataatcctgc aaacttaatg
    13021 gggatcaaag cgtttaaaaa gattttacct gacattttga gtgtggctgt attcgataca
    13081 tctttccact caacaatgcc aaagcaaaat tatttatata gcttaccggt gtcttattat
    13141 gaagatttca aagtaagaaa atatggcttc catggcacaa gtcatcgtta tgtgtcggaa
    13201 cgtgcggcaa aattattgaa caaaccgatc gaagaactaa aaatcatcac ttgccactta
    13261 ggaaatggtg tatcgatcac agcagtcgat ggaggtaaat ccatggatac ttctatggga
    13321 ttcacaccat tagctggggt gacaatgggt acacgttcag gtgatatcga tccagctgtt
    13381 ctcccatatt taatggaaaa attagagatt gatattgatg aaatgatcaa tgttttgaac
    13441 aagaaatctg gtctgctagg cctaacagga ttgtctagtg atatgcgtga tcttgaaaag
    13501 aactatgaaa aagaacatat tcgtttagct tatgacattt tcgtggaccg catccgaaaa
    13561 tacatcggcg gttatgtgac aacgatgaat ggtgttgatg caattgtctt tactgctggt
    13621 atcggcgaga atgacggaca tgtacgtagt gaaatcatca aaggtatgtc ttggtttggt
    13681 tgtgaaatcg atccagcgtt gaatgaattg cgtggtgaag aagtagatat ctctacaaaa
    13741 gaatcaaaag taagagtatt agtgatccca acagatgagg aactaatgat tgcccgtgat
    13801 gtggagcgtc tgcgcggcta agccaaagca ttcgttagga taaaagatga aacgcatgca
    13861 agagggaatt cttgcatgtg ttttttttgc ggagaagacc gcaaaatggt aatttaactt
    13921 caatttccct tcttttttac aggatcttct tagggaattt tgcttggaaa agtggtcaaa
    13981 agggaaaaga ctggcatttt atttattgat tttcaggaaa atcccaatat tttattttca
    14041 ttcatcttta taagggacat tacatatgta aaaagaaaaa acatttttag atcagcctcg
    14101 tgggctgtcg actttattct tcgctttatg tttagctaga aaaccgatcc gaaacattca
    14161 ttgatcgaat aataaaaact tgattttcca cctgtacttt gcctgaatcg tgaaagcaca
    14221 ggtatgaaaa tcaagttttt tgttgcgaaa aaacgtccta gtcaatcagt tggccgagcg
    14281 gctggcggct gatctgctcc tgatcaataa cgagtgcttc ttggaagtgt tgctgcaaaa
    14341 agtagcggtc atggctgact gctaggagcg ttccttgata gtcttgcagc atttcttcaa
    14401 tttcttctcg agcatagctg tcgaggtgat tggttggttc atccaaaatc aggacgttga
    14461 ttttttctaa aaagagtttc agcaacgcca aacggatttt ttcaccaccg gataaatctt
    14521 tgatccgttt acccacatcg ctttgataaa agccactttt ggctaattgc cgtcgagcga
    14581 cttcttcgtt tccagtcaat gtgatggcgt aagccagcaa acggctatcg ggatcggcaa
    14641 actgcagctg ctgcggcaaa taaccgattt gcaagctact acctaatttg atggtgccac
    14701 cgtaaggctt ttcctcaccg agtaataatt tgagtaaggt tgattttcct gcaccattgt
    14761 cgccgatgat ggctaaatga tctccacgat agactgcaaa actgctgtct ttgaatagta
    14821 cttgatcagc gtactgctgg tgcaagtttt gaatgatgaa aacttctttg ccagattttc
    14881 caccattaga caagctttgc agccgatttt tcgggggagg tggcggtttg acgagtgtca
    14941 gtttcgccag gcggcgttcc agttctttgg cttttttgaa gaaggactca ttgtctcctt
    15001 catgtgccca ctgacgaaaa cgacgaatca tgactttcaa ccgctggact tctttttgct
    15061 gcagttcgta attttttgtc agctcagcca agcgagcttc tttcaagact cgaaaacggc
    15121 tgtagtttcc gggatactca atcaatgcac cttcttccag ttcccatgtt tttgtggtga
    15181 cttgatcgag aaattgccga tcatgagaaa cgaccagaac cgattttttt gtattctgta
    15241 aatagttttc caaccagcga atactgttaa gatctaaatg attggtcggt tcatcgagta
    15301 aaagtaaatc ggcatcggct gctaaaattt tggctaaagc gactttaact cgttcgcctc
    15361 cgctaagttc tgttaaccgt gcttcctgac ggtgtggaat ccctaagcct tgcaagatcg
    15421 ctgcaatccg gtcttctaat acgtaaccgc ctagttcttc aaagcgatgc tgcagctcac
    15481 cgtaacgggc cagacacttc tctaagtttg tatctggttc tgtcatcaat gcttcgtagc
    15541 gactcatttg ctgtttgagg tcctgtatct cagcggcaga atggagcaag tagtggttga
    15601 cggtcgcttc ggaaactgta agttcttgtt cgaccgtccc gatctttaag ccttttttgc
    15661 gactgataac accgctgtcg acaccttctc tgccggtcaa gatttgtaaa aaggttgatt
    15721 tgccagttcc gttgataccg actaaggcaa ttttttcacc agcttcaatt tgacaggata
    15781 aatcctcaaa taatggattc ccgccgattg tttttttaat agaaattgct tgtatgatca
    15841 tgatctctct ccttgagacc actcaaaaag gccatggtta tccatggcct gtgttattta
    15901 aactcagatt atcatgagaa aaataccttg agatggccat tgattttacg tgaattcaac
    15961 tgttttggac aaacttgtcc cgtcaacagc aactgtaaaa ccaattgcat ggctaaatgc
    16021 gatatggtat cgttggtaat tttcattact tgttatttta gtcatgcgca tcatagtatt
    16081 ttccctcctt cgtgtagttg tcttttccag tgtacctgaa agtgtcagaa agttcaacta
    16141 gcgctgacat ttacttaata tttatcgatg tgtaattctg tcagagagct ttttttatct
    16201 gactgagggt aatgtctcgt ggaccacatc attgtattct gagctaaacc agcgttcagg
    16261 cgaaatcata tggtgtttga taaaaaactt tgtctacatg acttcctttt gccatttgct
    16321 taataccgtt gcattaaaca gctcttctct ttggaaatct caccaacatc gcgttcagga
    16381 gtatcgttgt ggttgcaatg gcaagcggta gcctttcatc aattcttgaa tgatttaccg
    16441 aatgtagcac ttgccttctc gcggtacccg aacccgaaat aaaattttag tttggcgata
    16501 aaatcggtat ccgccatcga tacttcatat tttttcgtat cagattcatc ggaaacttcc
    16561 acattgacga accaataagc tttggcatgc ttaggtcgtt ttaaaccaca ccagggcgac
    16621 taatcgccct ggtgttcact tttttttatt ttacttgaac tcattgactg ttttcccttt
    16681 attttaaaca ttataaagac cgatgagtgc ttgccgcgtc ttttgttttt atcacggttt
    16741 tttgtgcaaa aaaacctgcc gatagttggc aggtcgtcta cgtgttaagc tttggtgtgg
    16801 tctttagctg gcaattggtt ttctaaatca gttcggacaa caagcacatc gcaagctgcg
    16861 tttctaataa catattcaga aacagaacca ataaataaac gttctacagc attcaatcct
    16921 gtggctccta acataatcaa atctacttga ttatcttctg ggatttctct ggcaataatt
    16981 tgttttggtg agccatattc tacgacagag gttacattat tcaagccagc tttcttcgca
    17041 ttgctttcat aatctgccaa tgtttgcttt gccatttcag tagcttgttc tgcaagcatt
    17101 ccatcaaagg atgaaacggt ttggaaagaa cgtgtgtcaa tgacatgtgc taatagaagt
    17161 tctccgttat ttcggatagc tacattgacc gcctttttaa aagctaattc agcttcatca
    17221 gaaccatcta cggcaaccat gatttttttg tattgttgta acataagcat tcactcctca
    17281 agtcaatcgt attgtactct tttattgtag aacactttag ggaaaaagag aattatttac
    17341 tgctttggtt cagcaatttt ttttgcaaat cggatactga aggtggcaga aacaataatt
    17401 acgatcagta aaaagaccaa ggcaataaag cgttgattga aaaaagctgt taaaataccc
    17461 attacgccta aaacagcata tgcagctccg taaaattgta ggaatccttg atttttatca
    17521 tttttgttga tcaatacaaa aaagatatcc tgtttattaa ataaataata gctaacgaat
    17581 aataatacta aagtggcgat aatcattaaa atcctaatca tgaacaaatc ctccaaaata
    17641 agcaacaaat agaaaaagca atttgccaag agcaaattgc tttattcttt aaaagatggt
    17701 aatctccgtt aatgccgttg tttgtcctgt agtattgatc ccgcgaattc aagcaggtgg
    17761 gtcccatggt cgtagcttcg aactaccaaa tgataaggca tgttaccagc agtgacacaa
    17821 gacgggtccc aagatatcaa taaaaatgtt cggtcaacac acaaagagga caattcgaac
    17881 atcacagatt tcccacctct atagtagcat atgaatttaa aaagagcaat gagagagctc
    17941 gcagaaacag aaccgttttc ttgtctagtt ttttggagga tgctttttcc actcttttat
    18001 acggtaatac tgttgaccca gagcctgttc atatttccct gtatcttttg gctgataata
    18061 ctgagcattc ttcagtttat ctggtaaata ttgctgattg acccaagcct gatcaaaatg
    18121 atgaggatac tgataaccca cgccacggtt gagtgatttc gcacctttgt aatggctatc
    18181 tctcaaatga tcaggaacat ctccagcttt gccctctcga atatcagcaa gtgctgcatc
    18241 taatgccatg taggcagaat tggattttgg cgacaagcaa agatcgacga cgacgtctgc
    18301 tagtgggata cgtgcctcag gcaatcccaa tttttcagca gctaatacag cgttcactgt
    18361 acgagccgca gcagcaggat tacctaaacc tatatcttca taaccgataa ccatcagacg
    18421 ccggcaaata cttgccagat cacctgcctc aactaaacgg gcgagataat gaagtgcagc
    18481 atccacatca cttccacgaa tcgatttttg aaaagccgaa ataacgtcat aatgagcatc
    18541 tccatttttg tcatgggtaa gagcttttct ttggacacat tcttcaatga tcgacagtgt
    18601 aagatgaatc cttccttctt tatctgaagg agtcgatagt gtagccagtt ccagcccatt
    18661 caatgcactc cgcaaatcgc cgttcgttgc acgcgaaagg tgaagaagag catcttcgtc
    18721 taactggatt gcttgttgtc caaggccgcg ttctttatcc cttaaagcat gttcgactgc
    18781 caactggatg tcttgttctg ttaatggttt gacttcaaag atctgtgtac ggctgcggat
    18841 cgctggattg atggtaatat aaggattctc agtagtcgct ccgatcaaga tgattcggcc
    18901 actttctaaa tgaggaagca gaaaatcctg ttttgtttta tctaatcggt gtacttcatc
    18961 caataagagg atcactgtac cactcatttt tgcttcttcc gctacaactt gtagatcctt
    19021 ttttgaatct gtagcggcgt ttagcatgcg aaatgcataa ttagtagagc cggcgatcgc
    19081 acttgcgata ctcgtttttc cagtaccagg aggtccatat agaatcatgg aagagagcat
    19141 gcgagcatcg accatgcggc gtatgatttt tccttccccg actagatgct gctgaccaac
    19201 gacttcatcg atcgttcgag gtctcatgcg ataagccaat ggctgttgca taggattttc
    19261 ctccttcttg tttttacgtc ccttaattat atcatattta gcaagatgcc tagaaagaac
    19321 agccttctgt tgttttgttt tcatagctta tccaactaat attgtgtatg ataaagtagg
    19381 tgagaaaaat gacggataaa caaaagatag atgaattttt ttggaataaa acgacgccag
    19441 aagtggcacg ggatttattg gggatgtatt tggagcatga cacaccagaa ggaagattag
    19501 ctggatatat tgtagacgcg gaagcatacc taggtccaga agacgaggcc gcccatagct
    19561 atggtttaag aagaacaccg cgtgtgcggg caatgtatga aaaaccaggt acgatctacc
    19621 tttacacgat gcatactcat cggattttga acatcattac tcagccagaa gggattcctc
    19681 aaggagtaat gatacgtgca atcgaacccg ccgcaatgat tgatcaaatg agtaaaaatc
    19741 gtggcggaaa aactggtcca gatatcagca acggtccggg aaaattagtg gaagcattag
    19801 caattcctca agaactttat ggtcagtcta tagcagatag ttcgttacgc ttagtttttg
    19861 agaaaaaaaa gacaccaaaa aagatcaatg ctttgcctag aatcggtatc ccaaacaaag
    19921 gtgtatggac agaaaagccg ttgcgttttg ttgtgtcggg aaatccttat atctctttac
    19981 aaagaaaaaa ccaaatcgag aaagattggg gttggaggaa agaaaatgaa aaagaaggat
    20041 ctattaacat atttagatga aataattgaa aaacaagtga cagactatga tgtagcttta
    20101 gattgggata caaaaaatca tacgatcgag atcgttgttc gcttgtttgc agaaaataaa
    20161 gcacaatttg agattgatga tgcagaaggg atcgtttcgc aagaaccaat tatcgaattt
    20221 gaagacggcg tacttctttt taatcctcag aaatctgttt ttgatgaacg caattatcta
    20281 gcagtaatcc cttatgaagg aaaaaaaggg ttggcaaaat cagtagctga ttctttagtg
    20341 gaatatctaa atgaagtatt agctcaaggg caaagcgatt tgcttgattt tttggatgaa
    20401 gacgatgacg aagctgtatt cgagcttcac tgggataatc agcaactagc agaattagta
    20461 gaagtaaaac agcaaaatga agcagatact tatttaccgt acccaagtta ttaaggagtg
    20521 acagatatga aatggaatga agtaaaagta gaaactgaaa gtgaagcagt cgaagcagtt
    20581 gctaatattt tgatggaggc aggggcgagt ggtgtcgcga ttgaagattc tttagatgtt
    20641 gaacattttc agtcggatca atatggtgag attttagata aaaatgattt tacatcgctg
    20701 aaagaaggcg cactagtcat ggcttatttt ccggaaacga ctttcttacc ggaaatcttg
    20761 ccttttatca aagatcgtat cacacaatta cctgagtttg gtctagctat tggaaaaaat
    20821 aaagtaacag ttagtgaagt agaggaaagc aactgggcaa cagcctggaa gaaatactat
    20881 catccggtgc gtattacgcg aatgctgacg gttgcgccaa gctgggaaaa ctaccagaca
    20941 gaagatcctc tagaaaaaat tatcacacta gatcctggta tggcatttgg cacaggcact
    21001 catcccacga cttccttgac attgacagca ctagaaacag tcttgcgtgg tggtgaaaca
    21061 ttgctagatg tgggaactgg gtcgggcgtg ctaagtattg cagcaaaata tcttggtgca
    21121 aaagatgtct atgcctttga tttagacgaa gtagccgttc gttcggcgaa agaaaatatg
    21181 gatatgaatg aaatagccaa agatgtccat gtatcagcta acgatctttt gacaggaatc
    21241 gagattgaaa gtgatgtaat cgttgcgaac attttagcag atatcatctt attgatgatc
    21301 ccagatgctt ggcgtttact gaagcaaaca ggcacgctga tcgtttcagg aatcattgaa
    21361 gagaagaagc aaatggttct ggatgccatg ttagaacaag ggtttatcgt ggatcaaatt
    21421 ttccagcaaa aagactggta tgcaattatt ttgaaaaaac cagaggtgga atagatgcag
    21481 cggtattttt taaaagaatc atatgaatca aaagactatt ttgttttaga aggggaaaac
    21541 tatcaccaca ttgtacgggt tatgaggatg gaacccgagg atgaagtttt tttggcattc
    21601 aatgatcaaa tagccattgt tgcagaaatc acggagatta ctgaagaggc tgtttatctg
    21661 aaagaaatta aaaaagaaga aaatcaaaag gaactgccaa gttcgattac catagcgagt
    21721 ggctatccta aaggggaaaa gctggattgg atcgttcaga aatccactga attaggagcg
    21781 caagcttttc ttggtttccc ggcatcagcc tctgtcgtta aatgggatgc aaaaaaacgt
    21841 atgaaacgac aacaacggct tgaaaagatt gctcaagagg cagcggaaca atcccatcgg
    21901 cagatcgtgc catcagtaga gttattggaa acgaaacaag atttatttga gcggctagat
    21961 gattatgacg tggtcttggt tgcttatgag gaatcagcaa agcaaggcga aaaatcaaat
    22021 gcagccaaaa ttcttagtga aacaaaacca gatagtcgaa tcttagctgt ctttggacca
    22081 gaaggtggat tatcccgcga tgaagtcgat tattttgtag aacacaaagg gatactttgt
    22141 ggattaggac caagaatcct tcgtacagaa acagcacctc tttatttatt aagtgctgct
    22201 agctatcaat gggaattaat gaatccttct ttcagctaac gttcttcatt aataaaagag
    22261 tatcgacaga aagaattgaa aaaatagtca acaatttgta taatagaaac aattatttat
    22321 acaatggata cgaaaaatcg tttgaaaaag cttttaaaaa gcgaacattt ccgtaaaaaa
    22381 acgtataact gcacggattt tgggacaatt atcaaacaga tatttgtccc gattcccgtt
    22441 ttaccgaaaa aagaaagggg gatcgattcc tgtgacaaaa gaagaaattt taacgggacc
    22501 cggtgtcata aagctcgtta gtcaatacat gggtcccgaa catgtagcat ttgttgagaa
    22561 agcatgcgaa tacgcgactg ctgcacatga cggacagttt agaaaatcag gcgaacccta
    22621 cattattcat cctatccaag tcgcaggtat attagcagat ttaaaaatgg atccccatac
    22681 agtggctaca ggcttcttac atgatgttgt tgaagataca gaaatcactt tagaagatct
    22741 gagagaagaa tttggcgatg acgttgctat gttagtggac ggcgtaacca aattagggaa
    22801 aataaaatat aaatcccacg aagagcagct agcagaaaac caccgaaaga tgctgctagc
    22861 aatggctcaa gatttacgag ttatcatggt caaattagct gatagactac ataacatgcg
    22921 tacgttgaag cacctgcgag aagataagca aagaaggatc gctcaggaaa ctttagaaat
    22981 ctatgcgcct cttgcacatc gtctagggat cagccggatc aaatgggaat tagaagatac
    23041 agcacttcgt tatctaaatc caaaacagta ttaccgtatc gtccacttga tgcagacgaa
    23101 gagagaagaa cgtgaaaaat acgtaagtgg tactgttgaa gatattcgaa tagcgacaga
    23161 agagctgggg atttttgcag aaatctatgg acggccaaaa cacatttatt caatctatcg
    23221 taaaatgaaa gatcagaaaa agcagttcaa cgaaatttat gacttgctag ctattcgagt
    23281 gatcgtagat tcaatcaagg attgttatgc tgtattagga gcaatccaca caaaatggaa
    23341 accaatgccc ggcagattta aggattatat tgccatgcca aaagctaata tgtaccaatc
    23401 tttgcatact accgtgatcg gtccggcagg aaatccggta gaaattcaaa tccgaacaca
    23461 ggaaatgcat gaaatcgctg aattcggggt tgctgcacac tgggcctata aagaaggaaa
    23521 aaatgaaaaa gtagaaccag atggtatgac gaaacaatta agctggttcc atgagatact
    23581 cgaacttcaa gacgaaagct atgatgcttc tgaatttatg gaaggcgtaa aaggagatat
    23641 ctttagtgat aaagtctacg tcttcacacc aaaaggagac gttactgagt taccaaaagg
    23701 atccggacca ttggactttg catacagtat ccatacagat atcggtaaca aaaccactgg
    23761 tgcaaaagta aatggcaaaa tggtgcagct tgattacaaa ttgaaaaacg gagatatcat
    23821 tgagatcatg acttctccaa attcatttgg cccaagtcgc gactggttga aattagttgc
    23881 tactagcaaa gcaagaaata agatcaaacg tttcttcaaa gcccaagatc gagaagaaaa
    23941 tgtgatcaaa ggccacgaat ccgtggtcaa atgtattaca gatctaggat ttacgcctaa
    24001 agatattttg acgaagaaca aactgcaaga agcactcgat cgttttaatt atcaaacaga
    24061 agatgatctc tatgcagctg tagggtatgg agaagttagc cccttgacga tggccaatcg
    24121 tctgactgaa aaagaacgta aagaacaaaa aatcgagcag caaaagcaag aagcagaaga
    24181 aatcatgaat cagccgaaaa aagaacctga caaaatgaaa gtacgtcatg aaggtggcgt
    24241 tgtcattcaa ggggtagaaa acttactaat tcgtatcagt cgctgctgta atccgattcc
    24301 tggtgatgat atcgttggtt atatcactaa aggcagaggg atatccattc atcgtcgaga
    24361 ttgtccgaat gttcagcctg acaaaccaaa tgtagcagaa cgtttgattg aagtcgaatg
    24421 ggaagataca tcgaatacac gaaaagagta tgatgcagat ttggaaattt acggctataa
    24481 tcgttcaggc ttattgaatg atgtacttca aacagtcaat gcgctaacga aaaatctcaa
    24541 cagcgttgaa gcacggacga ataaagataa aatggcgacg atccatttga cggttggtat
    24601 ccagaattta tcccacctaa agagtatcgt ggataaaatc aaagcagtac ctgatgtcta
    24661 cagtgtacgc cggacgaatg gataggagga cagatatgag agcagttgtt caacgggtaa
    24721 gtaaagctgc tgtgtcagtc gatcagcaag aaattggaaa aattggctac ggcctacttg
    24781 ttttattagg cattcatgaa accgatacgc aaaaagatgt ggactatctg gtaaaaaaaa
    24841 ttgcacaaat gcgtattttt gaagatgaac aaggaaaaat gaatattagt gtagaagatg
    24901 tgggaggaca aattttaagt atctctcaat tcacactgtt tgcggatacg aaaaaaggaa
    24961 atcgaccaag ctttgtggaa gccgcacgac cagaaacagc tattccttta tacgaatcat
    25021 tcaatgaagg aatcagaagt cgcggcatca cagtcgctac cggacaattc ggagcagata
    25081 tgtcggtttc tctcgtcaat gatggacctg taacgatcat tattgattct aaaaacaaat
    25141 aaagtaatct ggttttcact agtaaaaact atgaaacacg tcgcttaagt tagactgata
    25201 aactctaact taagtggctt ttttgtctgc aaaaagcgct tcttttgtca gcagtcggaa
    25261 aaaaagttaa gcttaaatta tacaagttat tttttatagg aggttgctga agatgaaaga
    25321 agtaccagta gtagatatca aaatcacaga tttaaaaaaa ttgtatcaaa aaggatacaa
    25381 catgttggag gagttacgcc atgaagcgga cgctcccgtc gtcaaagcaa agattttcaa
    25441 taaagaagct atcacgatct acggatcatc agcagcaaaa gtattttatg atccaaggaa
    25501 tttcaaacga aaaggtgcta tgccaaaatt agtattgaag acactttttg gtcaaggtgg
    25561 agtacagact ttggatggag ctgctcatca ccatcggaaa aatattttta tggatctaat
    25621 gacaccggaa agaatggaag attatcatcg tatcttagat aagaatctga cacaagcact
    25681 tgaagcacag catggtcaat ttgaactatt tgatttaagt aagatggttt tctttacatc
    25741 tatttgcgag tgggcaggta tcaatttatc agcgatttct aaagatgagg tcgaaaaatt
    25801 agcagaatat caaatctcta tgatcagcgg aacgtttact tctccgattg atcacataaa
    25861 aggtgtcgaa aatcgcaaaa aatcagaaaa atgggcgcaa ggcttgattg aagaagctcg
    25921 acaaaatcct gtggctggaa aagaaaatgt ggcattatat gcttttgcca atgccacaga
    25981 tttagatggt cagcttttac ctctggaagt ggcagctgtt gaattattga acatcatccg
    26041 tccaacggtt gctctaactg tgtgggcggc attgatggga catgcacttt tcagtagacc
    26101 agatttgtat cagcaattaa aaaatgactt ttcgacgtta caagatccgt ttattcaaga
    26161 aatgagacgc tattatcctt tcttcccaat gctacctgcg atttcgctga aagaagtaga
    26221 agtagatggt tatcgtatac cagaaggcag ttgggttatt ttagatttat atggaacaga
    26281 ccatgatgaa cgaacagttg aagcgccaga ttcctttatg atcaaacggt atgtagggaa
    26341 agcaaaagat atttcctata aagaagaata tgagatgatt gcacaaggcg ggggaaattt
    26401 tcgacaaatg catcgctgtg caggagaatg gatcactctt catagtctac gtgtgttttc
    26461 cgatcagcta gtaaataagt ttgagttctc tgtacctgaa caagattgga cgattccatt
    26521 caatcagttt ccgacttatc cgaatagccg agcgctactt tacaagaatt agattagcat
    26581 aaacttatcg aaaaaaagga agatcaaaca gtgctgatct tccttttttc atattactat
    26641 aaattgataa ggtcaattga tcaaaatcca cgaaatgctt catcaatggt ttgataatct
    26701 tcattggata aatgcaaatc taaagcttgg acattgtcag atacttgctc tggacgctta
    26761 gctccaggta taactacaga cactcgtgga ttcttcatgt accaagctaa aacaagttgc
    26821 gcaggagtgg cttggtatct tttagcaatg gggcgaagta catcgacagc ggttaaaatt
    26881 tctctgaaac gttctccttg gaaatctgga tttctttttc gaggatctcc ttcaccaaat
    26941 tgtttttcct caccaagttc atattttcct gtcagcaaac cggaagcaag aggaaaatag
    27001 ggaacaaaac tgattttgtt tttttcaaga taagggaata attctttttc agcctgccga
    27061 tggatcaaac tgtatttatc ttctactaca tcgacatatc catctgcatt cgcttctttc
    27121 aattgttcta acgtgaagtt tgacacaccg actgctcgga tttttcctgc ttcttttaat
    27181 tcgtgcaatg tagcaactga ttcatttttt ggtgtgcttt catccggaaa gtggatataa
    27241 aaaatgtcga tgtaatcagt ttgcagacgt tttagtgcat cttccaccga ttgtttgaga
    27301 aactcaggag aattatcaaa tgttcgccct ttatttggta catgagcagc ttttgtagcg
    27361 atgacgacac gtgagcgatc atattctttt tccttcagta cctcgccaat cagttcttcg
    27421 gaacggccgt ttccgtaagc ataagcggta tcgatcaaat tgatcccgct attcaaagct
    27481 gtacgaacta cttgttttcc tgtctcatct tccaaaccgt caaaaagatt atgtcctcca
    27541 acggcatttg ctcctaatcc aagctgttct gcgtagactt gtgaatgtcc gattcttatt
    27601 tctgccatag ctgattcctc ctttttgtct agtctaaaac aaaataccaa aaagctgaac
    27661 taaaacgttt ttgtgagggg aaatagtcag atatctcttg actttgagac tggtttttag
    27721 taggttataa gtaatatcga atgtcaatga aagagcgagt aagtttttga aactttacaa
    27781 gagaggatca tcgaggctga aaatgatcta aagtttcatt agcctaaaga cactctggag
    27841 atggacgagt gaaaagccgt cccgcgtacg agcgttattc gtaagaggaa agagaaatct
    27901 ttcaaaatta ggtggtaccg tgtatgaatt tcattcaccc ttgttcatga tgaacaaggg
    27961 tgattttttt atttacatag ctaagaaagg gagagaaaac tatgagtttc caaagaccaa
    28021 aaggaacaaa tgatattttg ccaggcgaat cagaaaaatg gcagtttgtc gaagaaacag
    28081 cacgtttatt attcagagat tatcagtata atgaaatgcg tacaccgatt tttgaacact
    28141 atgaagtgat atcccgaagt gtaggggata caacggatat cgtttcaaaa gagatgtatg
    28201 atttttatga caaaggggaa cgccatgtga cgcttcgacc agaaggtaca gcaccaatcg
    28261 ttcgttcttt tgttgagcat aaattgttcg gcccagagta tgcgaaaccg ttcaaaactt
    28321 tttatatggg accaatgttt cgctacgaac gccctcaaaa aggacggtta cgtcaattcc
    28381 atcaaatcgg agtagaagca tttggatcag aaaaccctgc aactgatgtg gagagcatgg
    28441 tgatggcact tgatttcttc aaacagttgg ggatcacgca aatccggcta gtcatcaatt
    28501 cattaggtga taaagaaacg cgtcaagcct atcgtcaagc attgatcgac tatcttttac
    28561 cttttgaatc agagctgagt gaagactcta aacgccgcct tcatgaaaat ccattacgcg
    28621 tattggacag taaagacaaa cgagatcaaa aatttgtggc agaagctcca tctatcttag
    28681 attatctaag cgagcctgct caaaaacatt ttgataccgt tgtttccatg ctggatgcat
    28741 tggctgttcc ttatgaaatc gacagtaata tggttcgcgg actagattat tacacacata
    28801 cgatttttga aatcatgagt gacgcaccaa aaatgggggc acaagcaacg atttgcgcag
    28861 gcggacgtta tgacaatcta gtggaagaac taggtggtcc ttcaacacct ggcttcggat
    28921 ttgcaatggg tatcgagcga ttactcttga ctatggaggc agaggaagta gttatccctg
    28981 catttaatga gttggatgct tacgtggtcg cactcggaga cgaaacaaat atcgaagcat
    29041 tgaaagtggt ccaagcgatt cgaaactttg gtttttcagc tgaccgcgac ttcatgaacc
    29101 gtaaagcaaa agcgcaattt aagacagcag ataaagcgaa tgccaaatta gtattggtga
    29161 tcggtgaaga tgagcttgct agtcaaacga ttaatgtcaa agcaatggca agccgaaaag
    29221 aaaaaagttt tccattagaa gagatatatg aacattttga tgaagtctat gatgaaatga
    29281 ctttattcga gtaggaggaa aaaaataaat ggcacaaaga acgatctatt gcggattagt
    29341 ttccgaacaa caattagaac aaacagttac attaaaagga tgggtacaaa aaagacgaga
    29401 ccttggggga gtaatcttta tcgacctacg tgatcgtgaa ggaatcgttc aaatcgtctt
    29461 taatcctgaa atcaacaaag aagcatggga aatcgcggat aaatgtcgca gtgaatacgt
    29521 gatcgaagtg actggggaag ttagaagacg cggagaaaat gcagtaaatc ctaaaatggc
    29581 cactggtgaa ttcgaagtga ttgcaagtga aatcgtgatt ttgaatcaag caaaaacccc
    29641 gccatttcaa attgaagatg atcaagcaat cagtgacgaa atacgtttga aatatcgtta
    29701 tttggatctg cgtcgtccaa aaatgactaa aaacatcaaa ttaagaaatg cgacaacaaa
    29761 agcgattcgt cattatttag atgataatga ctttctcgat atcgaaacac cttacctagg
    29821 gaaatctaca ccagaaggcg caagagacta cctagttcct tcacgtgtcc atgctggaca
    29881 tttctatgcg ttgccgcaat ctcctcaatt gtttaaacaa ttgctgatga atgctggatt
    29941 cgaccgttat tatcaaatcg ttcgttgttt ccgcgatgaa gatttacgtg gagaccgtca
    30001 gcctgaattt acgcaagtag atattgaaac aacgttctta acagcagaag aaatccaaac
    30061 ttacacagag ggattgattg cgaaggtaat gaaagaagtt cgcggtatcg aagtcacatt
    30121 gccatttcct cgtatgacct atgatgaagc aatggctcgc tacggcagcg acaaaccgga
    30181 tacacgtttt gatatggaat tgatcgatct aagcgataca gtcaaagaag tagaattcaa
    30241 agtcttccaa atggctcttg aaaacggcgg tgtcgtgaaa gcattgaatg ctaaaggcgc
    30301 tgcagatcgc tactcacgaa aagacatgga tcagttaggt caatatgtag gacaattcgg
    30361 agctaagggg cttgcatggc tgaaagtaga agaagacgga ttgaaaggtc caattgctaa
    30421 attcatggga gaagcaacag aagcaatcat taaagcaaca gatgcaaaac caggtgactt
    30481 gctgatgttt ggcgcagata aaagtgaaat cgtcgctgct gcattaggtg ctatccggac
    30541 acgtttagga aaagaattag gactaattga tgaatcgaaa ttcaatttct tatgggtaac
    30601 tgattggcca caatttgaat tttcagaaga agaaggtcga tatgtctctg ctcaccatcc
    30661 attcacgatg ccaaaagaag aagatatccc gttattggct gaagaccctg caaaagtcta
    30721 tgcagaagct tatgatatcg tcttgaacgg ttatgaacta ggcggaggtt ctatccgtat
    30781 tcatactcgt gagcttcaag aaaaagtact agaaactcta ggctttacaa aagaacaaat
    30841 ggaagaacaa tttggtttct tgttaacagc tttagattac ggattccctc cacatggcgg
    30901 tattgcatta ggattggacc gttttgcaat gttattagcc ggagaagata atattcgtga
    30961 agtcattgcc tttcctaaaa acggaaaagc agcagatgtg atgagtgatg caccaagtat
    31021 ggtttcgcct ttacaattgt tcgaattgaa tattgatgta acgaatatag aagaatagcc
    31081 gataaaaagg taaaaaggat ttgtcgaata cgcttctttt gacaaccttg atttaattga
    31141 aaaacacttt ataatgaagc cataacatat aaagcggggc ttttccatga aaaaaacatt
    31201 cgaaaattgg gcttatcatg attacacgac caagctttcg gtatctatcg tttatgcgtt
    31261 gatcgcatca gtcgcattga atttctttta tcagccagga catatttatt caagtggtat
    31321 tactggtcta gctcagattt tgacgacttt gtcaaaatca ctgataggat tcgaagtacc
    31381 ggtatcaaca actttgtatt tactgaatat acctttgttt gtattggcgt ggttaaaaat
    31441 cgggaagaag tttacaattt tcacttttat cacagtcact ttgacctccc tttgtatcca
    31501 tattttaccg gaaacagtcc tatctaccga tccaatcatc tgtgcgttgt ttgggggggc
    31561 tgtgatggga ctaggtattg ggctagcgtt gagaaacggc ctatcatcgg gcggtctaga
    31621 tatcgtgagt atcacgattc gtaaaaaaac aggaaagtcg attggttcta tttcgatcta
    31681 cttcaatgca ttgatcgttt ttgttgccgg aattctattt ggctggcaat atatgtttta
    31741 cagtacgttg tctattttcg tcagtggaaa agtaactgat gtcgtttata ctaaacaaaa
    31801 gaagatgcag gtaatgatcg taacgaaaca tccagaaaaa gtagtaaatg aaatccaaaa
    31861 ctgtatgcgt agagggatca cgatcatcaa tgaagcagaa ggtgcttata agcatgataa
    31921 gcagacagta ttgatgacga tcgttacacg atacgaactg cctctactta gagaagcaat
    31981 gaaagcttct gatccacaag cttttgtaag tattgcagaa aacgtccaga tacttggtcg
    32041 tttctatgag gatggcattt agatcgagtt tgacaaaaat tcatgaaata ctgctgtaga
    32101 cagacgttcg tttgtctgca gtttttttct accatagtag aatcagcaaa gaaaaactgc
    32161 ttttttattt gaaattttct taaagttgaa aaaaagcatg tttatttttt taaatttatg
    32221 ggatgataga aaaaaatagt agagaaagga cacgattcta cttgctattt tcacttaata
    32281 cccgtatatt acgaatgaaa aaagtttatg aaaagttgga atattgaatg gaaaaacaaa
    32341 aaaggttaaa aaacagccgc tacatcactg ggtttgatgg gctccgttca ttagctgtgg
    32401 tgggtgttat cttctatcac cttctgccaa ctagtatgaa aggcggctat ttgggcgttc
    32461 cgattttttt cgtcgtatct ggttatttga tcacggatct gctgcggcag gaatgggaac
    32521 aaaatggaaa aatcaatatt tggcaatttt atgtcagaag aatgaagaga ctctatccgg
    32581 cacttgtttt tatgcttgtg acagcttcgg cttatatcac tttgtttcag cgagggctgt
    32641 taaacaatct tcgaggagtt gtggtcagca gcttactgta tgtcaacaac tggtggcaaa
    32701 tcaataacgg tttatcttat ttcgatcgat ttgctaatga gtcaccgttt acgcatatct
    32761 ggtctttagc agtagaaggt caaaactatt tggtttggcc gatcttgttt gtattgttga
    32821 tggtatttgt tcgtaaaaga aaatggatct tttatactgt gttaggtaca tccttcctct
    32881 ccgccatctt gatgatgatc ttgtatacac caggaggcga tccaacccgt gtttattacg
    32941 gtaccgacac tcgtctgttt tctatatggt tgggaagtgc gcttgcgttt gtttggccaa
    33001 gtacgcgttt aaagaaaaac atcccgaaac aagcgaaaaa tattctgaat atcgctggag
    33061 gaatctcgct tttggcactg attctttgct tcttcttttt agatgatcat tacaatttca
    33121 tctattacgg aggaatgctc ctagtgagtc tgttatgcgt agtgctagtt gcagtgacag
    33181 cacaccccgg agcaagtttg aatcgatggc tgacaaatcc agtatttact tggattggca
    33241 aacgaagcta tggcatttat ctgtatcagt ttccagttat gattttttat gaagcaaagg
    33301 tgactgatat tgccgatcat gttttgttgc atactttagt ggaaatcaca ttgattctaa
    33361 tcatcagtga actttcttat cgatatgtgg agcgtccgct agcacgtttt gattatggtc
    33421 gtacattcca agtgatgaaa ggatggttca gcaaaccgat catcagtaag agcaaaccat
    33481 gggttgttcc aggcacatta cttgtagcaa ttgcactcgt tggtttcgtt acggcaccga
    33541 aaaacgctgt aacagccgat caaaaacaac tgcaggcaaa aatcgctgaa agtaaaaaat
    33601 tagcagaaga aaccagaaac aattcagaaa acaccactag ttcggttgac caagcgatcc
    33661 ttgataagta cgagttgtcc aaggcacaag ggaataaggc acaaaaatta gaactaacgg
    33721 catttggtga ttcagttatg ttggatgctg caacggatct acaagaactt tatccaaaag
    33781 tagtggtaga tggagatgtc ggacgacaat tatatgccag tgtaccgtac attgaaaaat
    33841 taaaggaaca agatttgttg aaagacactg ttctgatcgg actaggaacg aatggctcat
    33901 tctcggaagc tcagtttgat agtgtgatgg atgcaatcgg tgatcgaaaa gtatactggg
    33961 tcaatgtccg agtaccgacg aagcgctggc aaaatgacgt gaatgcgatg cttgaacaga
    34021 tgacgaaaaa atacgacaat atgacgctga ttgattggta cagctacagt aatgaccata
    34081 gtgactggtt ttatgatgat caagtccatc caaatcctga agggatgaca cattacattc
    34141 acttagtcag cgaagccttg ctaggaggaa gttcaagtac gaacagcagt gagacaaacg
    34201 tgacagaatg atagctacaa aaaacttcag gcaaaaacat ttgtttttgc ctgaagtttt
    34261 tttatattta ccgtgttttt aacattcata tagattcagg aagcaaaacg gttgagtgtg
    34321 tctgtaaata acgttctttt tgttttcggg tgagtttttt gaatgcagct tctgcttttg
    34381 ttgcttcgct tcttgaagaa aatacttctg catggatcat cttaacaggt cttctttttt
    34441 gaagacgagt atatttagca ccagtaccat tgttatgttc ctctaatcgt ctagaaagat
    34501 cggttgtata tccaccataa aatgtctgat ctttacagaa taatacgtaa aaataatgtt
    34561 catttgccat agagcatctc acgaatctct gcagtgtatt ctccatccat attgtaagtg
    34621 atcaatggtg gagcgacttt gaatccgtct aatttgcctt ctttgattcc ttcaactaac
    34681 aaaatatttg cctcttttcc aggttttggg taaacaaatt ggatcctttt aggtgcaatt
    34741 cgatagtctt ccatgacctg aatgatttct aagaaccgat caggacgatg gaccatagta
    34801 aagcgtccat ttgtttttag tagcttactt gaaatatcga tcacttcttc taacgtcgta
    34861 tgaatctcat gacgggcgat tgccaggtgt ttattagggt tcgttttatt tgttggcagc
    34921 cctttgaaat aaggtggatt acagacgact aagtcacaag aattatgtcg aacgacagat
    34981 aagctgtctt tcagatcgat ggtgtggaca gtcatttgtt catttaaatg gttaagcgcg
    35041 atactacgtc ttgccatgtc tgctagacga ggttgaagtt cgatagcgtc gatagccgcc
    35101 tgggttcgtt tacttaaaaa aagtccaact gcgccatttc ctgcacaaag gtcaacaatc
    35161 ttcccttttt taggtacacg ggaaaagtga gcaagcagca cagcatctaa agaaaaagaa
    35221 aacactttgg aactttggat aatcttgata tcttctgcgt ataattggtc gattcgttcg
    35281 tcttcgtgaa gcatggattc cctccaaaat gaaaaaaata gtcgtctgta ttatactatt
    35341 tttattaaaa agaaccaagg tggcttgtca aacctctatt ttttatgcat actatgacca
    35401 taagaaggga aaggaagaga ctatgtttta tcgatttatg cgcggggtag ttcgagtact
    35461 attagcctta gttaatggaa atgcccgcta tgagaataaa gaagttttac cgcaagatga
    35521 gaattacata ctagttgctc cccatcgtac ttggtgggac ccattatatc tggcggtagc
    35581 tgcagcaccc aaggaattca gctttatggc aaaagaagaa ctgttcaaaa atccagttct
    35641 gcgatttata ttaactcgat ccaatgcttt tccagttaaa agagaaaatc caggaccaag
    35701 cgtcattaaa acacctgtaa agattttgaa atcgacaaat ttgagtttga tcatgtttcc
    35761 cagtgggaca agacatgcta cagaacttaa aggcggaatg gcattgattt ccagaatgtc
    35821 taaagcgaaa atcgtgccag ctgtatatca aggtccgttg acacttgggg atctgttcaa
    35881 gcgtcagaga gtgacagtgc gtttcggcga accaatcgac ttatcagata tcaagaaaac
    35941 ggataaagaa ggtatggcgg agatcgaacg acgaacacaa gcagcttttg acaaattgga
    36001 caaagaagtg aaccctgatt tcaaatatga agtcaaaaaa aaataaaatg ataagtttat
    36061 gcgtgggcgg tcttttgaat ctactgtcct gttcagttca gactttttca atgaaaacga
    36121 gggagagacg tcaagtctgc cctctttttg cgttccaata tgtaaaatag aagacaggtg
    36181 tcatgtaatg gatcaaagtg ttataataat tagaaggaca ttttttgagg tgattgaatg
    36241 cgttttttac atacagccga ttggcatatc ggaaaaaaac tacaaggtta tgacttgatt
    36301 gaagaccagc gtcatgtact atcttctata ctagatatcg cgaaagaaga aaaagtagag
    36361 gcaatcgtga ttgccggaga cttatatgat aggagtgtcc cagctgttga tgccgtagaa
    36421 ttatacaatg aattgatggt cgattggaat ttaaaggaaa agatcccgat ttttgctata
    36481 tcaggaaatc acgatagcag tacgagactt tcagcgggta cgccatggtt cagttattcg
    36541 aactattttt tgcgaacaca gctagcagaa gcatttgaac cagtcgagtt taaaaacgta
    36601 caatttttct ttcttcctta ctttgaacca attgcagcga gattatattt tgaagatgac
    36661 aatatccgaa cgatcaagca agcggtagcc aaggtgatag ataaaatgca ggagctattt
    36721 gattcagaaa aaaaacaagt attgattagc catttttttg taatgggaag tgtaaaatct
    36781 gattcggaaa cgaaaatcga agtaggcggg ttggattcga tccctggtga actacttaca
    36841 ccgtttgatt atgtagcact aggacatttg cataatcaag ctgctttgag acaggataat
    36901 gctcgctata gcggttcccc attgaaattc tctttatctg agatcaacca aaaaaaaggc
    36961 gtatggatcg ttgacctgac aacaggcctg gagttgaatt ttagggagat cgaacccctg
    37021 catgatattg aagaaataac agcgagtttc aaagaattgc tggatcctgc cttttatcag
    37081 tccgtcaatc gggagaatta catctatatc tatctcactg atagaacgat catcccgaat
    37141 atgatgaacc agcttcggca aatatatcct cgtattttgg ggattgaacg ggcaaacggt
    37201 cgagcagaac aaacaaagcg caaacgaaaa gtactagaaa tgaaagatcc tcaaaaatta
    37261 gcagaagcat tttttagtga aatgacggaa gagcagctaa ctgcaaacca agaaaaatgg
    37321 ctcggagaaa cgttgcaaga aatcatggaa atggaatgat caagaatgaa accgaaaaaa
    37381 ttacaactta aaaattttgg tccttttatc aatgagaccg tagatttttc acgtttgaca
    37441 gaagcaccgc tttttttgat cagcgggaag acaggagctg gcaaaacgac gatttttgat
    37501 gggatcacct tcgcactttt tggtgaaact tccggaagac ttcgctctgg aaaagagatg
    37561 cgttctttgt ttgctacacc agacgaagaa acaagcgtga ctttttcttt tgaacatcag
    37621 cagatgagct atgaaatcga acgaaaacca gaacaagttt tagcaaaacg aaaaggaaac
    37681 ggaacaaaaa aacaggcagc caaagtaacg ttgaccattt ttgatgggaa aggaaaagaa
    37741 ataaaacaaa tcacaaaacg tacagaagca gatcagttga tcagagaact gatgaatctc
    37801 gatgcgaaac agttttctca aatcgtttta ctaccccaag gagaattccg taattttttg
    37861 gtttcatcaa gcagtgataa agagactgtt ttacgtcatt tattcggtac tcaatttttc
    37921 caacagttca atgagcgatt aaaagaaaaa gcaaaacaac agcaacaaaa attggatcat
    37981 ttagagcaag agctgcatct tctgcaaaaa aggtttgttt tagtcaaaga agaggaacca
    38041 gcagcagggt ttgaagccgt tttagcccaa tggacaaacc atcaggcaat actcgaaaaa
    38101 caaatcacgg aagaaaaaga agctctaacg gaacttcagg aaaaacaaaa gcaactagaa
    38161 aattcctact atcgttttga aagcttgaaa aaaagttatg aagaaaaaac gatattaata
    38221 aacaagcaaa aggaattagc tgctcaaaaa gacgaaatag aggaaaagaa acgatggatt
    38281 gggtattatg aatttacgca acgattagtc gaaccaatcc agcgcctaca agaaataaaa
    38341 gttgaaaaaa tagaactgtc acaaaaaagt agtgagcaaa caaagaaact agcgatgact
    38401 acgcagaatt atgaagagtg gcaaaaaaat gaagcaaacc gcgtacgccg acaaacaaaa
    38461 atcaaagaag ctgctcaaaa actgcaaaac aaacagatga tggtgccaat tgtcgaatcc
    38521 ttacgtagta aaaaacaaga cgcagaacaa ttaacaattt cattggataa agaagaattg
    38581 aatgccgaga agctgtatca aaatctcgct atctatcagg aaagaactcg aattcttcaa
    38641 gaaaatttac tggatcagga aaaactgcaa gaagatcgtc ttgcttttgg acagttaaaa
    38701 tatctagatg aaaagcagca agaaatagaa aaacaaaaag caaaagacac tgggtcgctc
    38761 gcacagataa gagaagaact caaaaaagca actgaagaaa taacaaaaaa tagtcagcta
    38821 ttgaaagaac agacagaaca agcagaacag ctgaaaagcc aatgggcaaa gcagcaaatt
    38881 gctcgtcttc agcttttttt gcgtccaggg gagccatgtc ttgtttgtgg atcaacggaa
    38941 caccccatac aacaattaaa acacgaacag ccaaataagg aagatatctt aaaagtagaa
    39001 caacaactgg cagaagctga gtctgctgta gaaagaactc agcaaatcat tgccaaaagt
    39061 gaagcacatg aaaataatct tcaacaacaa ttatcagatt tagaacaaaa aatcatggca
    39121 acgaatatac aattggctga acaagaagaa cacttgcaac aggctttgaa aaaggcattt
    39181 cctgagataa cagaacaaaa aacaaaaaca actcttgtag taatcgaaca atctttagat
    39241 agacgaaaaa gcaagatggc caatgcaaga atagagctgg aagaaataag acaacaagca
    39301 gaaaagctcg aacaacaatt agctgtctac aaacagcagc agacagaaaa aaataatcag
    39361 ctccagcaac tgcaaggaga aatcaaaagt ttagaacaac aattgcaaga tacggatagt
    39421 aactgtttag aagaggagat acgacgactt gaaaaacagt tagaagaaga ctcgaatcaa
    39481 ctagctgttg agcagaaagc aggagtgcaa ttgttgcaag atcttcatac gcttcggaca
    39541 cagcttgatc ttcaacatga acaaatagaa aaaatagatg aaaaaataga aaaggaacaa
    39601 gaaagaatag ctaaaaagtt agaaagccag tcgtattttt caacaattga agaaatctgt
    39661 gcatttgcac aacagcaagc acaatataaa gaaaaccaac gtacagttac ttcttatcat
    39721 gaagagcaat tagtgacagc tgaccggctg cgtcaattag aaaatatcca gctaactgag
    39781 acattcccag atacagaaga gatcagccaa cagttagaag aaataagacg tatgatcgaa
    39841 ataaagcaag aaaagatata cggtttacaa gaacaaaaaa ttagtaatca aaaaatctac
    39901 gatgaatgtc tttcgattta tcacacaagc cagacgaaat tagatgagct tacgcaatta
    39961 cagcagcttt cgcaaacatt caacggagaa aatcctaaga agactagttt ggaacggtat
    40021 gtgcttcagg tttatcttca agaagtgtta caagtagcaa atgatcatct ccaacgttta
    40081 acgaaaaatc gttatcaatt tgagctagcg gataccattg gcagttatcg agggaaaacc
    40141 ggattagaaa tcaatatcta tgatgatcag gcaggtatgt ctcgaagtgc gcataccctg
    40201 tctggcggtg agagttttat tgcagcactt tctttagcat tagctcttgc cgatgtgatc
    40261 cagacacaag ctggtggtgt agcaatcgat gcattgttta ttgatgaagg atttgggtca
    40321 ttagatgaag aagctttgga aatggcaatg gaagctttag aaacgatcga aagtgaagga
    40381 agaatgatcg gcatcattag tcatgtaaga gaattgaaag aacgagtatt acaacaaata
    40441 agaattgata cagaaggtag cggacaaagc aaaatacgct atcaacttgg ataaaaaggg
    40501 agattggtaa ctatgaaatg gagtattcca gaaaaagtca ttgcacgtgg acgtcaatac
    40561 ttgcaagaag gccgagtgtt atcggttgtc cctgaccaag aagaaaacgt gtggcatgca
    40621 gaagtcttgg gcagtgaatt gtatatggtt gatttggatg gtacagcaaa agagatagat
    40681 tactgccaat gtccctattg ggaggaacat aaatattgta agcatacagt tgctgtggaa
    40741 ctttatttac ggtcgaaaaa tctatctcgt atcatgaaaa aagaccagcc aatgctagaa
    40801 ccggtaaaag caacaagtga gggagaaatc attgcaaaag gtttttctcg tctaaaaaca
    40861 caaaacaatc ctcaagcggt gcaacctttg attattgaat accaagtaga aacgattgag
    40921 actaatcagt atcacccaga gctttctatt ttggcacttt acttgcgtat cggttcattg
    40981 gcatcaccga aaaaaacata tatcgtaaaa aacatctatg attttttaca agcttacaca
    41041 gaaaaagaaa cttacactgt taataaacag taccagtttc gtctagataa acgggcgttt
    41101 caaaaagaag atcaaaaaat actcacgcat cttgcaggaa gtgctcagac gcatcaatta
    41161 ttaggtacga acggcttaca agtcaaagga aaactagata aaagatatct gcttctccca
    41221 gtagaacaag cccgcttcct gttagagcag atgaatcaga ctacacgttt gcatctgcag
    41281 ataaatgaac aaaacgatca aggaatacaa ttttccgatc aaaacaaacc tttatcattc
    41341 aaagtggaaa aatttggaga agattatttg ctgcaagcat tgaatgattt tgattttttc
    41401 tttatgcatt atcagtgggg agttatccaa gggacatttt attctctgac gaaatatcaa
    41461 caaacggttt atcaaacttg gaaacaattg attcgccgat tggagaaacc agaagtagtg
    41521 ttcaagaaaa aagaattacc gtctttgttt aaagagatta tccctctgct gtcagagatc
    41581 ggcgacgtag ctattgcccc tgcagtagaa gaggaagtag cagatatccc cgtcgaattt
    41641 attttctttt tccgaaaagc aaaaggaaaa atccaagctc gagtcgattt cgtctatggc
    41701 gaagtcatct attcgactga cccgaagcat gaggtctcga cagaacaggc ttttcaggtg
    41761 ttacgtgaca cagtagaaga gaaacgagta cttgatctat tcagaatgta tcgctaccag
    41821 aaaaatgaaa ctggttatga acgtccattg ccaagcgggg aagaactata tgcctttttc
    41881 cgcacagaac tatctgtctt ccgtcagttt ggtgaagtca gacttggaag aaaattgcga
    41941 gagttgtatt tagatgcgca taaacatcaa ccgaaaatcg aagtaaccga gaccggttca
    42001 tggctcgata ttcgattcga tgtgacaggg atagaggagc aagaaatcga tcatgtcctg
    42061 caaagcttgc tgagaaatga tgctttttat acattagaaa atggtcaggt tctttctttt
    42121 gattcagaag aatttcaaca aacaagccaa gtgttgcaac aattacggga atcgatacgt
    42181 acagaagaag gaacgatcca tgtacctaaa aatcaaggat tgatcattca aaatcaattg
    42241 gaaaaaagca atgctacttt tagtgaatcg ttccaaacga tggtacaaga tctcattcat
    42301 ccagaaagat accaggcaca gcttcccaaa ggattgaatg caacgatgag agattatcaa
    42361 aagcaaggtt ttcgatggtt gaagatgtta ggtcattatc agtttggcgg tattttagcc
    42421 gatgaaatgg gacttgggaa aacactacag acaattgcct ttttactttc ggaaaaagaa
    42481 gaacggaaaa gcttctctgc tttgatcgta gcgcctgcga gcttaattta caactggcag
    42541 gcagaggtga gaaaattcgc cccttcttta agtatccaag taatcaatgg gaataaaaaa
    42601 gaacgtgaag aattactggc taaagacaca gatatccgag tgacttctta tgccagctta
    42661 agacaggatc tagctaatta tcagtcgcaa aaaattgatt atttgattct tgatgaggca
    42721 caaatggtca aaaatagcag tacgaaaact gcacaagcat tgagagaatt agctgtacct
    42781 cagcggtttg cactgagtgg gacaccaatc gagaataatt tagaagaatt gtggtcattg
    42841 tttgcaacga ttatgccagg atttttcccg actaaaacaa aattcaggga acttgccgca
    42901 gaagaaattg cgcaaatgat tcgaccgttt attttacggc gagataaaca aactgtgttg
    42961 aaagacttgc cggaaaaaac agagatgaat ctatattctg cacttacaga agaacaaaaa
    43021 actgtttatt tagcttactt gcgtcaaatg cgtgaagaaa tcacatcaat ggattctgaa
    43081 gcattcaaga aaaaccggat cgggatactt gctggtctga caagactgcg acaaatttgc
    43141 tgcgatcctc gtctgtttat tgaagattac caaggcggtt ccggcaagct cgaacaagta
    43201 aaagatctac tggtagctgc caaagagaac aaacgaagag ttttgctgtt ttctcaattt
    43261 acagggatgc tgactatctt gcaagaagag ttagccgagc tagggatctc gacattttat
    43321 ttaagaggca gtacgaaacc acaagatcgg ttaagtatgg tagacgcatt taatgctggc
    43381 gaaaaagatg tttttctgat ttccttaaag gctgggggaa ctggattgaa tctcacggga
    43441 gcagatacgg tcatcttgta tgacctttgg tggaatcctg ccattgaaga acaagctgct
    43501 ggacgtgcac atcggatcgg acaaaaaaat gtggtagaag tgtggcgtat gatctctgaa
    43561 ggaacgattg aggaacgcat ggattcactc cagcaagaaa aacgcgaact gttccaaaaa
    43621 gtgatccaag gtaatgaaga acagttgacg aaactgactg aagaagacat ccgcatgatt
    43681 ttaagtgtag gagaaatcga ggagtaagag actcgaaaaa gcgttcagta atgggtaaga
    43741 aagcctttct aaagaagcga ttgtttattt gatttaaaaa atagcaagga ggctgggatt
    43801 ccattttatc ccagcttcct tgctatcatt tttttatttc tatttttaaa ggttatacaa
    43861 agattgtctt cattgtctct aacagctgat caaaagaaga aatctcgata tcgcaaattg
    43921 gagtctgttc aattttgcgt tctcgatggt tgaaccaaag cgcttgccag ttagcacttt
    43981 ttgcacctag aacatcattg tcgtaattat cgccaacgta taaggtctct tctggaagca
    44041 tatgaaattc tttctcagct agctgaaaaa tctctttttc tggtttttgg aaacctgttg
    44101 cttgagaaat gagcatattg cgggaaggaa tccaattgtt caattgaagt tgcttcagtt
    44161 tttttgtctg atggtcagtc ggcccatttg tgataattcc taatgggaca ttcttttcct
    44221 tcaagaaatc gagggttttt ttgacctcct catgcaagca gatattatca agttcttcct
    44281 cgtaaatttt ttgaaataag aggccatctt cttccgtaat atgagggtag tctaaatctt
    44341 ttagtgattg gctgatccga tgtgctcgca tatattctag tgtccaatct cctgccatta
    44401 cttttgggaa gttttcatca ctatgatggc ggaaccggat atatagtgga tgcatatctg
    44461 catctgaaac aagcggaacg actcttttga ccgcgtttcg aaatggctgc tgctgatcat
    44521 acatcgtatc atcaacatca aacacaactg atttaatcaa aattttacac gcctttcggt
    44581 tttcattgat tttttcacaa atccttgact attgtaacag aaaaaattgt ctaaaaaagc
    44641 tgataaataa aggtttttct tactatattt ctatttcctt gacagaataa ggtttttata
    44701 tttcagtttt tttcactaaa aatcgtgaaa cacgtcaata agtttgatga acaaattaat
    44761 cattatggta gaagtcgatt gatttatttt aatgcaatct ctgtggaatt ttcgaaagat
    44821 ttattttttt aatacagtct caatataact attcaaacat aaaataacgt tatttgattt
    44881 atttgtttat agtaaatttt atttagtaat cattgcttta aaatactacg cttatataac
    44941 agagaaaata agcgttataa taaagaacag tattatttaa aatgacagaa aacattacta
    45001 ttaaagaaat aaaaaacata aaaaatctta agaaatcggt ttcatttagc ttttgagata
    45061 aaataatagt acagttttat acgctgtttc atatgtttag aaaatttgaa ttagatttga
    45121 aaactctaat ttttaaatga taaaaaaagc gagaatgctg gttttactgt atattaacct
    45181 tagataactt tcattttttt aaaaaaatta tgaaaaaaag gtagctattt aaaagttgat
    45241 tatgtataat agaaacagat gggagattat tatgatcttc tgttacaaaa aagtgattct
    45301 tatcacgaaa aggagtggtg cacgttgtta acattatata cttccccaag ttgtacttcc
    45361 tgcagaaagg ctcgtgcgtg gttgcaagaa catcagattc catttgttga acgtaatatt
    45421 ttttcagaac cattgaacag ttctgaatta aaagcgattt tacaaatgac agaagacgga
    45481 acagaagaaa tcatctcgac acgttccaaa gtattccaaa aactgaacat ggatttagat
    45541 gatctgccgt tacaagaatt attggaactt gtgcaaaaca atcctggatt gcttcgccgt
    45601 ccaatcatga ttgatgataa acgtctgcaa gttggtttta atgaagatga aattcgtcgg
    45661 ttcctaccgc gcgatgtacg acaattagaa ttacgtcaag cacaattgat ggctggatta
    45721 tagaaggata atacgaaact tggaaaagac tattacaaag atagtttttc caagtttttt
    45781 tgtttttaca aaaagtattc ttgattagat gtataaaaag gtagtagaag atgatgattg
    45841 cacgaaaaat agagagggaa aattagctga aaagtcggct atatagtatt cgtctgccaa
    45901 aaatgttata gttaaataag atagcttgaa aggttgaaaa atccgctggt tttattaaat
    45961 tcgcttaaca gcgagatttt ttttctttac atttaagctg atttcgctac aatgaatcta
    46021 tattaaacat tgtggcaaag tgaggtgtag caaaatggaa atggaacata ttaatgaaaa
    46081 tacgatccgt gtactaattg gtcatgaaga tttagaggaa cggggcgttt catttttgga
    46141 tttacttggc aatcacaaag aaatcgaaaa tttcttttat agtattttgg aagaagttga
    46201 tatcgaagag gagttccgag gaagcgaagc tgtcactttc caagttttgc caaaaggaga
    46261 cggattagaa ctgtttatca gtaaaaatct tccggatgaa gaaatttctc aatcagaaga
    46321 ttctactgtt gattcagaag atgtgagtga ctttcttcgt cagcagatta ttggcagcga
    46381 tccggaggaa gaattatctg gaagcgccct agaagaacag gaaagacaag caacttttgc
    46441 ttttgaagat tttgaacaag tgatccaact tgcaacagat gtcgaactag agtctgcttg
    46501 gactgatttg tatttattcg aagagcgcta ttacctagtc gttcacttct ggatggaaaa
    46561 tctcaaccaa gcggatgtag agaatcagat tgctcgaatt ttggagtttg ctgaaaagag
    46621 cgatcgtaca ccagaaacat tgaatgaata tggacaatgt ctgatggcgc gtaatgctat
    46681 cgagcgaacg aaattttatt ttgtataaat cagcattata gtaagagact gtgacaaaaa
    46741 ttgtgtcgca gtcttttttt cgtataaatg tgttcaaaag cttgcatctg cggtaacaag
    46801 cccaaccaaa cgaaaaatat tgggaactat ttttgtttag ctgttcttat tacttgatgc
    46861 agaatagctt tttcacgccc tcttttttcg tgtaaataaa ttcttagacg taaataagtt
    46921 cagagggaga aaaatgctgc ttgcaatgaa tgaatataat aagtgctgtt ttgctaaaca
    46981 tgcagaaaaa ggaaagaaat attattgtcg tggctgtcga aaaagagtga ttttgaaaaa
    47041 gggaaagaaa aaatgtgctc attttgccca tcaaaaatca gataactgtt cggtctttag
    47101 tgaaagagaa tcaaaagagc acttacagtt aaaggaatgc ttcatggatt ggctgggaca
    47161 gtcagtagaa ccagtatttt tggaagccta tttgccgagg ttgcggcaaa ggccggatat
    47221 tttgttggcg aatctagcaa tagaaatcca atgttctaga ctgtctcacc agagatttat
    47281 tgaaagaacc caaaattatt tgaataacag ttaccaagta tggtggatac ttggtaattc
    47341 ttttcttgga caatcgcaat tctcattgat agaaaaaagt tgctgctact ataatagaaa
    47401 aagaggagtg cattgctgga aagctgatct caagaaacaa aagctttatt tgtatcatca
    47461 tattacagag actgtatcag ggcatatttc cttttgcagt agctgctgga ctttttctag
    47521 tagggacctt aaagaaatat tcactagtaa cgaaattaaa atgaaccaaa tgaaaaaaat
    47581 agaaaaagtg agtgaagatg ggaagaaatg gctgacacgc caacttattc acaagcaaaa
    47641 gaatacagta tccatacaag aacaatgcta cttgcgtcat aaacatcttt tgcatctatc
    47701 atcgtggatc tatcaaaaaa gtcggttttt cttttattta caagaacagg tttttcttta
    47761 tcgaatgttg tacgaagaag ccttggaaaa ccagaaagtt cctgatttca actcatggct
    47821 ttgccaaata aaagaacata aaagggaatg gctttttcct atgattgatg aagagatggt
    47881 ctatcagcag ttttttaacg aatgcataca cttgtcttct ttgaaaaaat agtaagtata
    47941 aaaataggct aggaatcggt tttctatgta aaacaagatt tagttatgct aaaattgatg
    48001 gaagcagaca catgaatgag tagaaaagtt agatgctgtg acatgtggat gttagactat
    48061 gatctatttt gatagataga gctaagggaa gaaaaggagt ggatttcatg gaagtaaagc
    48121 agttgccaaa acgagaagaa ttgcctgaaa atttaacttg ggacttgacc aagatctttt
    48181 caagcgacca agagtttgat gagaaatatt tggaattatc agaagagtta aaacaatctg
    48241 aaaaacacaa aggaacactt gatcaaggcg cttctcaatt tttaaatgcg attgaattcg
    48301 tattgagggt ttatcgccaa actgaagtca tttatgtata tgcgcacctt aaaaacgatc
    48361 aagacactgg aaatacagat taccaagcgc tttatgcaag agcaagcagt ctgttttcga
    48421 aagttagtga agccgtttcc tggtttgaac cagaaatatt gcaattgtca gatgaccaga
    48481 tttggcaata tttcaaagaa gaaccaaaat tggaagtcta tcgccattat atccagcaaa
    48541 tagtagataa tcgagcccat gtcttatctg ctgagcagga atctcttctt gctggagcag
    48601 gtgaaatctt tgatgcttca agtgatacat ttgctgtttt gaataatgca gatctagttt
    48661 ttccaacgat tgaaggagaa aatggtgaaa tagtccaatt atctcatggc gtgtatggtc
    48721 agttgctaga aagcacggat cgaagggtgc gcgaagcagc atttaagggg ttgtacagtg
    48781 tttacgaaca atttagaaat acatttgctt ctactttagg cacacatata aaaggacata
    48841 attttaaagc gaaagtccgt aattacagct ctgccagaga agcgtctttg agcaataatc
    48901 atattcctga aagtgtatac gatactttgg tagacgtggt aaacaagcat ttgcctttgt
    48961 tacatcgata catggaatta cggaaacgtt tattagaagt ggaaaaactg cacatgtatg
    49021 atctttatac accggtctta ggggaagctc caattacctt tacgtacgaa gaagcaaaag
    49081 aaaaagcttt agaagcactg aaaccaatgg gtgaagaata catggccatc gtagaaaaag
    49141 cattctctga acgttggatc gatgttgtcg aaaataaagg gaaacgaagc ggtgcttatt
    49201 cttcgggaag ctatgacaca aatccatata ttttattgaa ttggcatgat acgctggatc
    49261 agctatttac gcttgtccac gaaatgggac atagtgttca tagttatttc actcgttcga
    49321 accagcctta tgtgtacggc gactactcca tctttttagc agaaattgct tcgacaacga
    49381 atgaaaatat cctaacggag tatttattgg aaacagaaaa agaccctcgt gtacgggctt
    49441 atgtactcaa ccattacttg gatgggttta aaggaacagt tttccgccag acacaattcg
    49501 ctgaattcga gcatttcatg catacggaag atgaaaaagg tgtgccattg actagtgaat
    49561 acctaagtga tagttatggt aaattgaatg caaaatatta tggtccagca gtcgaagaag
    49621 acccagaaat taaattcgaa tggtcacgga taccgcattt ttattataat tactatgttt
    49681 tccaatactc gactggcttt tctgctgctt cagcactggc gaagaaaata ctaaaccagg
    49741 aaccagaggc actagagaac tacttggctt acctaaaagc aggtaacagc gattatcctg
    49801 tggaagtaat gaaaaaagca ggagttgata tgacacaggc tgcatatatt gaagatgcaa
    49861 tgtcgatgtt tgaacaacgt ctaaatgaat tagaagaatt gatcgatcgt ttataaggct
    49921 ttctattttt gattttctcg aatagaaacg aaaaaaatac gtcggctctc ttgtaaggga
    49981 gtcgacgtat tttttt
//
LOCUS       DBNICI010000004        45535 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.004, whole genome shotgun sequence.
ACCESSION   DBNICI010000004 DBNICI010000000
VERSION     DBNICI010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 45535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.004"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            300..929
                     /locus_tag="AC1ZCC_000194"
     CDS             300..929
                     /locus_tag="AC1ZCC_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628483.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(1053..2162)
                     /locus_tag="AC1ZCC_000195"
     CDS             complement(1053..2162)
                     /locus_tag="AC1ZCC_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1628484.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            2371..2793
                     /gene="mgsA"
                     /locus_tag="AC1ZCC_000196"
     CDS             2371..2793
                     /gene="mgsA"
                     /locus_tag="AC1ZCC_000196"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HIQ1628485.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      2980..3078
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            3116..3652
                     /locus_tag="AC1ZCC_000197"
     CDS             3116..3652
                     /locus_tag="AC1ZCC_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HIQ1628486.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(3692..5455)
                     /locus_tag="AC1ZCC_000198"
     CDS             complement(3692..5455)
                     /locus_tag="AC1ZCC_000198"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1628487.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(5458..7173)
                     /locus_tag="AC1ZCC_000199"
     CDS             complement(5458..7173)
                     /locus_tag="AC1ZCC_000199"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1628488.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(7952..8176)
                     /locus_tag="AC1ZCC_000200"
     CDS             complement(7952..8176)
                     /locus_tag="AC1ZCC_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628489.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            8367..11144
                     /locus_tag="AC1ZCC_000201"
     CDS             8367..11144
                     /locus_tag="AC1ZCC_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIQ1628490.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            11225..11644
                     /locus_tag="AC1ZCC_000202"
     CDS             11225..11644
                     /locus_tag="AC1ZCC_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIQ1628491.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            11656..12126
                     /locus_tag="AC1ZCC_000203"
     CDS             11656..12126
                     /locus_tag="AC1ZCC_000203"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIQ1628492.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            12143..12922
                     /locus_tag="AC1ZCC_000204"
     CDS             12143..12922
                     /locus_tag="AC1ZCC_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HIQ1628493.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            12909..13730
                     /locus_tag="AC1ZCC_000205"
     CDS             12909..13730
                     /locus_tag="AC1ZCC_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HIQ1628494.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            13747..14796
                     /locus_tag="AC1ZCC_000206"
     CDS             13747..14796
                     /locus_tag="AC1ZCC_000206"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIQ1628495.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            14809..15813
                     /locus_tag="AC1ZCC_000207"
     CDS             14809..15813
                     /locus_tag="AC1ZCC_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIQ1628496.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            16446..17465
                     /locus_tag="AC1ZCC_000208"
     CDS             16446..17465
                     /locus_tag="AC1ZCC_000208"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIQ1628497.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            17508..18389
                     /gene="galU"
                     /locus_tag="AC1ZCC_000209"
     CDS             17508..18389
                     /gene="galU"
                     /locus_tag="AC1ZCC_000209"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-alpha-D-glucose metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HIQ1628498.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            18689..19066
                     /locus_tag="AC1ZCC_000210"
     CDS             18689..19066
                     /locus_tag="AC1ZCC_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628499.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            19672..20286
                     /locus_tag="AC1ZCC_000211"
     CDS             19672..20286
                     /locus_tag="AC1ZCC_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIQ1628500.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            21059..22798
                     /gene="yfcC"
                     /locus_tag="AC1ZCC_000212"
     CDS             21059..22798
                     /gene="yfcC"
                     /locus_tag="AC1ZCC_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HIQ1628501.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            22955..24139
                     /gene="iadA"
                     /locus_tag="AC1ZCC_000213"
     CDS             22955..24139
                     /gene="iadA"
                     /locus_tag="AC1ZCC_000213"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HIQ1628502.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            24250..24453
                     /locus_tag="AC1ZCC_000214"
     CDS             24250..24453
                     /locus_tag="AC1ZCC_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HIQ1628503.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24554..25216)
                     /locus_tag="AC1ZCC_000215"
     CDS             complement(24554..25216)
                     /locus_tag="AC1ZCC_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1628504.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(25229..26731)
                     /locus_tag="AC1ZCC_000216"
     CDS             complement(25229..26731)
                     /locus_tag="AC1ZCC_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1628505.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            27279..28958
                     /locus_tag="AC1ZCC_000217"
     CDS             27279..28958
                     /locus_tag="AC1ZCC_000217"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0004534 - 5'-3' RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HIQ1628506.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            29085..29564
                     /locus_tag="AC1ZCC_000218"
     CDS             29085..29564
                     /locus_tag="AC1ZCC_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HIQ1628507.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            29783..30463
                     /locus_tag="AC1ZCC_000219"
     CDS             29783..30463
                     /locus_tag="AC1ZCC_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIQ1628508.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            30471..31802
                     /locus_tag="AC1ZCC_000220"
     CDS             30471..31802
                     /locus_tag="AC1ZCC_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HIQ1628509.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            31817..32779
                     /locus_tag="AC1ZCC_000221"
     CDS             31817..32779
                     /locus_tag="AC1ZCC_000221"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIQ1628510.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            32890..33228
                     /locus_tag="AC1ZCC_000222"
     CDS             32890..33228
                     /locus_tag="AC1ZCC_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HIQ1628511.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            33307..34014
                     /locus_tag="AC1ZCC_000223"
     CDS             33307..34014
                     /locus_tag="AC1ZCC_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HIQ1628512.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(34117..35598)
                     /locus_tag="AC1ZCC_000224"
     CDS             complement(34117..35598)
                     /locus_tag="AC1ZCC_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1628513.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            35907..36443
                     /locus_tag="AC1ZCC_000225"
     CDS             35907..36443
                     /locus_tag="AC1ZCC_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIQ1628514.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            36521..37891
                     /locus_tag="AC1ZCC_000226"
     CDS             36521..37891
                     /locus_tag="AC1ZCC_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HIQ1628515.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            37958..38293
                     /locus_tag="AC1ZCC_000227"
     CDS             37958..38293
                     /locus_tag="AC1ZCC_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628516.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(38374..39231)
                     /locus_tag="AC1ZCC_000228"
     CDS             complement(38374..39231)
                     /locus_tag="AC1ZCC_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1628517.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            39338..39853
                     /gene="tadA"
                     /locus_tag="AC1ZCC_000229"
     CDS             39338..39853
                     /gene="tadA"
                     /locus_tag="AC1ZCC_000229"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HIQ1628518.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            39875..39961
                     /gene="ffs"
                     /locus_tag="AC1ZCC_000230"
     ncRNA           39875..39961
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="AC1ZCC_000230"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            40170..41555
                     /locus_tag="AC1ZCC_000231"
     CDS             40170..41555
                     /locus_tag="AC1ZCC_000231"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HIQ1628519.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            41592..42269
                     /locus_tag="AC1ZCC_000232"
     CDS             41592..42269
                     /locus_tag="AC1ZCC_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HIQ1628520.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            42277..43041
                     /locus_tag="AC1ZCC_000233"
     CDS             42277..43041
                     /locus_tag="AC1ZCC_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HIQ1628521.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            43043..43702
                     /locus_tag="AC1ZCC_000234"
     CDS             43043..43702
                     /locus_tag="AC1ZCC_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HIQ1628522.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(43732..44241)
                     /locus_tag="AC1ZCC_000235"
     CDS             complement(43732..44241)
                     /locus_tag="AC1ZCC_000235"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006644 - phospholipid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A family protein"
                     /protein_id="HIQ1628523.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(44353..45351)
                     /locus_tag="AC1ZCC_000236"
     CDS             complement(44353..45351)
                     /locus_tag="AC1ZCC_000236"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIQ1628524.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
BASE COUNT        14763 a         7761 c         9429 g        13582 t
ORIGIN      
        1 gcctgccgtt taactctaag tcacaaggaa caatttcaaa aaacagcttc tcatgttttt
       61 gagttagttg gtcttattac ttgatgcaag cttttgacca ctattatgcc gaacaaggag
      121 atgtgacaca aattttgtca catctcctat ttttgtttct tgatcaaaaa attcttatac
      181 ttgatactca cactcatttt gtatggattt agaaaagaat atgtgtcttg acagttatta
      241 gtaagtgagt tatactaagt cgacatttat gtgtcaagac atattaggag agagaaaaaa
      301 tgagaaaaaa ttcagttcga aaactaacga tttctgcttt attgattggg atgggggtca
      361 ttatcccaat ggtcatgcct aaaatcatga ttccaccagc ttcctttact ttggcgtctc
      421 atgtgccatt atttattgcg atgtttttta caccaggagt tgcagtggct gttgcgctag
      481 gtacgacatt tggtttcttt ttaacaacgc cggttatcat cgcattgcga gcattgtcac
      541 atcttgtgtt tgcgttgatt ggggcttggt acttgcaaaa gcatccgagt atcgttttaa
      601 aaaatggaca gtttactttt gctaattggc gtttccaagg gttcaatttt gtgatcggtg
      661 tgattcattc tttagcagaa atgctagtag tcagcatctt ttactttatt ggtaatatgc
      721 ctgaaacata ttattctcaa ggatattttt atacagtatt catcttgatg gggattggtg
      781 ggcttatcca cagtttagtt gattataata ttgcttattt tatagcagga acactcagca
      841 aatattttga tatccccgta tttacggcag caaaaaaaga aaatcggaaa gagtcgattc
      901 aagggtacgc gaagaaaacg acatattaga catgatctct caaaaaaaaa actctcatct
      961 agtaatggat aataaatcct ctaagatgag agttttttgt cctaaaaaga gctgcaccaa
     1021 catcttctgg cacagctcta acagggggca atttatggaa gtttgatgac ttgttctgtt
     1081 tctttatcaa agaagtggcc tttgctgatg ttgaaggcaa ggtcgactat ttcgcctggt
     1141 ttatggaaat ctcttgcatc tacttttgag atgaactcag tagaaccagt ttttgtgtaa
     1201 agcatcgttt cagctccaag taattcggaa acgacaactt ctgatttcac tacggcttca
     1261 ggtgaagctt caagggctac ttgttcactg tggatgtctt ctggacgaat accgaagata
     1321 acttcttttc cttcataacc tttttcaact aatactttgt tttttccttc aggaatacga
     1381 agcttaagcc catgtccatc tgtgataaca ccatttttca atgtcacatt aaagaagttc
     1441 atggcaggag atccaataaa gccggcaacg aacatattga cgggtgtatc ataaacttct
     1501 ttcggtgtac cgatttgttg gataaatccg tctttcatga taacgattcg gtcagccata
     1561 gtcattgctt ctgtctgatc gtgagtaaca tagatggtcg ttgtttcaag acgctggtga
     1621 agtttggcaa tctcagcacg catggctaca cgtaatttag catctaaatt tgataatggc
     1681 tcatccatta agaagacttt tgcatcccga acgattgccc gtcccaatgc cacacgttgg
     1741 cgttgtccac cagataaagc agctggtttt cgtttcaagt attctgtcag acctaagatc
     1801 tctgctgcat tgtccacacg ttttttgatg tctgctttat catatttacg taatttcaag
     1861 ccaaaagcca tattatcaaa aacagtcata tgaggataca atgcgtagtt ttggaaaacc
     1921 atggcaatat cacggtcttt aggtgccacg tcattcatga cttttccgcc gatatttagt
     1981 tctccttcag agatatcttc cagtcccgcg atcattcgca aagtagttga ctttccacaa
     2041 ccggaaggac caacaaaaac gataaattca cggtctttga ttgatagatt aaaatcagtt
     2101 acagaataat tttctgcatt gtcatatttt ttatatacgt ttttcagagc catttctacc
     2161 ataatacgtt ccacactttc ttttttagtt ttattaacta tttaaagtat aaatgaaagc
     2221 gttaactaac ttcaatgtca tgttgaacaa gaattttttc ttttttttgt caatgtgcta
     2281 gttttaagct ttaataaata tccataagaa ataaaaactg atacactgga gtaaataagt
     2341 atgttaacaa tatcaaaagg agtgtctctt atgaaaatcg ctttgatcgc tcatgaccga
     2401 aaaaaaccta tgatggtgaa attagcaaca gcgtataaaa caatattgga aaaacatgaa
     2461 ctttttgcta ccgggacgac tgggcagagg atcacggaag caaccggctt gccgatccat
     2521 cggtttaaat ccggaccgtt aggcggggat cagcaaattg gagccatgat ttcggaggat
     2581 cagttagatt tggtgatttt tcttcgagac ccgctggctg cacagcccca tgaaccagat
     2641 gtaacagcac ttatccgttt atctgatgtc tatgagattc cgttagctac gaatatcgga
     2701 acagctgagg tgctactaag aggactggaa gccggcttcc tagattttcg aagaatcgtc
     2761 catgaaatag acaagcatcc actttcattt tgatggataa gcgtatggaa aaataacgtt
     2821 taatgttcgc ctatatgtag ctataaaagc taaacaaact gataatatgc gatataatac
     2881 gaacgtattg gataatataa acaataatat tagtttttcg tatttgcagt aaatgtgaaa
     2941 atatgctata gtttctgtgg gaaaagatcc caaaaataat acagagtaga aaataaagcg
     3001 ttaagtgcta agaggatggg atgttgcctc ttggacgaag gacgtaatgt tcgcggtttt
     3061 attttcgcat tcgctgcatg atacgatgta gcagctctta aaggagatgc taaaaatgaa
     3121 gaaacaccgg ttagacgcac gtatgattgc tattatgggc ttattgattg cattgatggt
     3181 cactttgtcc cgactggtag caatcgagac cccatttatt aaaatcagtg tcacatttat
     3241 tccacaagtg atcatgggca ttctttttgg tcctttttgg tcagggatag gcgcagtttt
     3301 agctgattta gtaggaatgg ccctcttctc gaaatctgca ttctttattg gatttacatt
     3361 gaatgcattt attgaaggag caatctacgg attctttttc tatcgaaaag agatcacttg
     3421 gaaaaatgcc attctggcta cgcttagtgt cacgctgatc atcaacttat ttttgacacc
     3481 attatggctt gcgttgatgt accatgtgcc attgttcagc tgggtcgttt gggcgccccg
     3541 cctgttaaaa acagtaatat ggttaccgat tcaatcaatt gccatttatt acgtaggacg
     3601 tagtattcct tacaaaaaaa ttttaagaag tttagcgatc cacgctaaat aaaaaacaag
     3661 cagtcttccc atcccagaag actgcttgtt tttattgtgc tgtgaattgt gcttgataga
     3721 gccgtcggta ataaccatct ttttgaagca gctcatcatg ggtgcccatt tcgacaatag
     3781 ctccctgatc catcactaat atctggtccg catttttgat tgtggacagg cgatgggcaa
     3841 tcacaaaact ggtttttcct tccatcattg ctaagaaggc ttgttggatt tttttctctg
     3901 tcaatgtatc gactgagctg gtagcttcat ctaagatcaa cataggggga ttactgatca
     3961 tcgttcgagc aatcgtcaac agttggcgtt gtccatctga tatctttaac ccttgaccgc
     4021 caatcttagt atctaatttt tccggcaaac gttctacaaa ttcatacata taagaggctt
     4081 ttagggcatc attgatttct gcgtcacttg cttccggatt cccaaaacga agattttctc
     4141 gcaatgtact gtcaaatagc caagtatctt gtaacaccat tccaaaactt ttacgtaatg
     4201 tatcacgttg gatagagcgg atgtcatggt catcgactgt aattgttcct tttgttgtgt
     4261 cataaaaacg catcagcaaa ttaacaagtg tggattttcc ggcccctgtt tttcctacta
     4321 tagcaatggt ttgacctgga taagcagtaa aattgaaatc ttcgatcaaa cgctgcttgg
     4381 ggtcataaga aaaagaaaca tgatcaaaag agacttttcc tttgatattt ttgggttcca
     4441 aggcataggc atctggcaaa tcaggctttt cttctggctg atcgatcaaa tcaaatgctc
     4501 gttctagacc agaaaaagcg gtttgcagtt gtgtcgtgat tccagaaagt tcgataaatg
     4561 gtttagaaaa ctgacttgag tagatcgtaa agctggaaat cacaccaaca gtgattgacg
     4621 aaccactctt caatgccaat aggccgccaa ctaaaccaat cgataaataa gcaaggtgat
     4681 ctacaaaacg agaagacggg tttgtcaagg aagaagaaaa ttgtgcccgt tgtccttttt
     4741 catatagatc agcatttagc agttcaaatt gctgttgact agcttcttct tgctgaaaag
     4801 ctttcacgat tttctggtta ccgattcgtt cggtcacaaa gcccgagata tccccaataa
     4861 tcctttgctg tctatcgaaa tttgtttgtg aagcacgagc aacgatccaa ctcacaagga
     4921 aaataattgg tgtagaacaa agaacgacca atgtcagaag tggacttaga cgcatcataa
     4981 aaataaacga aagaatgatg attgccactc cagaaaataa ttgattgaag gccgcagaaa
     5041 ttgcaatgga aatattgtcc atatcatttg taaatcgact gacgatattt ccgtgggagt
     5101 tttgatcata ataacgaagt ggcaatcgat tcagatgagc aaatgcatct tttcttaatt
     5161 ttgcaacgga taagtaagct aaccgattgc ttaacacttg gatgagccat tgactgacta
     5221 cgttgattaa aacgatacca ccgaatagaa taagtaaatg gaacagtcga ttgaagcgga
     5281 cctgtccttc tccaatcatt tgatcgatac tttgaccaat ttgtaacgtc atcacaactg
     5341 ttgcaatccc attgatgatt cctagtatca cagctagcca aatgtctttt gcataggcag
     5401 taaggtaagg aatgaatcgg cggagcgtct gcaaggataa tttttttact ttcataacta
     5461 ttcttcctcc tgtgatgcga cgatttcctg atattctgct gattgagcaa gtaattcttc
     5521 atgggtaccc tgagcagcaa tcttaccttg atccatcact aagatatgat gtgcttcttg
     5581 aatagagcgg acacgttggg agataataat cactgtactg ccaagataag tctgtagcgc
     5641 cttacgcaaa tccaagtctg tttgataatc caacgcactt aaagagtcat ctaaaatcag
     5701 taatgctggc ttggcaatca aagctcttgc aatcgtcagt cgctgttttt gtccgccaga
     5761 gaaatttttc ccattttcca tgacttttgt atctaaacca tttggaagct gacggacaaa
     5821 ttcttctgcc tgagcgattt tcagagcttc ccaacattct ttttctgtgg cgtctttctt
     5881 accccattga aggttttcac gaatagtccc ggtaaatagt acagaagttt ggggaacaag
     5941 agcaatttgc tgacggagat gatccagctg ccaagaacga acatccattc cgtccattat
     6001 aactgtaccg gaactgacat catagaaacg cggaatcaat tgtgtcaaag tcgttttccc
     6061 gcttccggtt ggaccaatga ttccaaggac tgtatttcgc ttcagctgaa atgtaatatt
     6121 ttggagagaa agaccagaat caggagtata acggaagtca acatggtcaa aagcgataat
     6181 tgcttcagaa gaaaaatcgg gagtagtact gacctcagct tcaagaatcg aattttctgt
     6241 gtctagcacc tctttcaccc gatttcctga agcttcggca cgtgtgaaga tcacgacgag
     6301 gtttgaaaca acgatcagtg ccaaaagcat ttgattcata taattgatca aagcaagaac
     6361 ttgtccttgt tgcaagctac caatatttac tttgatcccg ccaactgtca gcaaacagat
     6421 gatcccgaca ttcatgatca aggttgtagc aggtgataat agggcagaga tattggaaac
     6481 acgtaaataa ttgttcgcta gatcatttgt gcttttatcg aacttctctg tttctttttt
     6541 tgtctgcgca aatgcgcgga tgacacgaac accgctaaga ttctgactaa cgtgttcatt
     6601 gagctgatct agtttttctt gcactttttg gtagagagga acggtttttt tgatgataaa
     6661 aaataaaatg attgagaaaa taggcaataa tgctaagaaa aacagaccta cttcccagtc
     6721 gatcacaaaa gccatgacca ctgacccgat acttaaaaac ggtgctctga tcaataaacg
     6781 aatgaccatt gctaaagcta gctgaagctg attgatgtcg tttgtcattc gggtgatcaa
     6841 ggtatctgtt ccaaaatgat tcaactcgtt gtgagagaaa gtattgattt ttttgattag
     6901 ctgattacgt aactctgtac caaatccttg tgaagctact gaagcatagt attggcaaat
     6961 gatagcacaa atcagaccaa tcacagacat agcgaccatc cataagccca tttcaatgat
     7021 ttttcctcgg tcattcattt ttagtccatt atcgatcaag gaagccatca ttaaaggcag
     7081 gacaagttcg aagacagctt caagaaattt gaagaacggg cccaaaatga tttgcttacg
     7141 atagtctttt gcataacgta ataactgaat catatgattc ctcctattat ttatttgtcc
     7201 acaattgaaa aaacaagtac taccacattg tagcttagga aatttagaat gaaaagagaa
     7261 caagcctttt cataaatgaa agttttatta tgaagaagct ttagaaattg aatagactat
     7321 tttttttaaa aaaaagaaaa tgagaaaaca tgccagtctc ttcattcata ggaacgtcta
     7381 ttttacttaa tttttctata aatgaattct gcataaaaaa tatatcaagt aaatgtttat
     7441 agatgaaact ctatttactt cttttttaat ttgttcttgc aatctttttt gctacgtaat
     7501 ttttgtaaaa aagtgggttg agagaaatct aattgatttt caaagaagga gtatgaaaat
     7561 gagaaaaata tttattcgtt tattgtgggt actatcttag aggaataaat aatagtggag
     7621 aaaactatgg tttggcgcag attctatcag gtttgatgga acgaagacaa cctcccgaca
     7681 tcaagtcaaa agccacttga gattttgatc tgaaaataaa aaaagaaaac aaggtaggaa
     7741 catcaatgtt tctaccttgt tttcgtaatc aatgggaaaa aactaagagt ggtgtacgta
     7801 atcaataaaa aggatggtga caacagtacc tctttactct ctgaaagcat cataactcgt
     7861 ttttcctcat tatgccaata aattgttgtt ataccacata taaggatttg taatagctaa
     7921 aaacagacag gaaagttgaa atctgtgaat cttattttgt atggaaggat ttatagaacg
     7981 caaaaatcaa gataaagatg aataaaaatc ctgtttcggc tatatattgc gtatctacgc
     8041 caataaaacg agtttgttct gtcatagcca tataaagcaa aggcaaaaat acaactacag
     8101 cgagataaga tccgatgatt tctcgaaaag ttttccagga agtatgtttc ctgtttttat
     8161 atttatccga tttcattgaa tagtcatagc ctccttattc tttcatatgt tgaaattcca
     8221 gtataaaaac tggaaggaac tgtacgtttt ttagaataaa aattataagg gataagtagg
     8281 ctgctgtcta taataagata tatttttcaa ttctttatta aagattttat tgacaaaatg
     8341 aatccgtttt ctatgatgga ggaggggtga ataagttgtt gaagaaggaa attttatgtt
     8401 acttagagaa ccagacagcc tttttcgatt tagaacatat gaacgaggtc tttacagcac
     8461 aaaggttagc tgatcatttt ggggtaaaaa gaaacacagt cagtcattat ttgaaccagt
     8521 taaatgatga gcaactacta gtcaaaatca attctcgacc agtgatttat ttccacaaag
     8581 aagcttttga aaaacaattt tttaagttac gcacgaattt ttatttttct atcgatgatc
     8641 tcaaagagga acagcctttt ttcgcgcaac caaaagactt attctctttt atgatcggat
     8701 acgacgccag tttgaaagaa agtatcgaac aaatcaaaac agctctttac tatccagatg
     8761 gcggattacc acttttgatt acaggcgaaa gtggcacagg taaaagttat ttagttaatt
     8821 tggtctatca gtattgtctg cttcatgatc tgctagaaga ttctgcacca ttcatcactt
     8881 ttaattgtgc acaatatgca gataatcctg aacttttgac tagcaatttg tttggacatg
     8941 ttaagggagc gtacactggt gcagaagaaa ataaaaaagg ggcttttgaa gcagcagatg
     9001 gtgggatctt gtttttagac gaggtacatc gcttaacacc agaaggtcag gaaaaactgt
     9061 tcacctatct tgatcaaggt gtgatttatc gtatgggaga acctaatttg tcacgccgga
     9121 tcaaaacacg tctctttttt gctacgactg aagagattac tagtcatttc ttaacaactt
     9181 tcattcgaag aatacctatc cgaatcgaac tacctgcact taatcagcgt agtcgtaatg
     9241 aacggctaga actgatttac tctttttttc tcgaagaaca aagaaaaatg gggagccctt
     9301 tgtcagtcag tggaccagct ttgtcacttt taacaagtca gaaattatcc ggaaacatag
     9361 gagagctgaa aaatatcgtc aaagtttcag cagcaaaagc atatgctgaa cagcgtgaac
     9421 agcctgaaat ccatgtcacg atccatcatt tgcaaaaaga actacttgcg cagcctatta
     9481 ctaaaaatac agcacagcaa ggaatctata ttacgcaaga ccagacctta gagcaattaa
     9541 tggagaagca acagcccgaa cagcagcgaa tcgttaaaag ttttgagcaa atcctaattg
     9601 atttcaagaa aaataactgt ctgctatatt cttgcgaagg aaaattaaag caagaagtga
     9661 ttcaattatt tgattttttg ctgtttgaga ccagtagaca agaaaaacac gagctgctgg
     9721 tttattggac acaatctatc cgagagacct ttcgacaaat ggaaactgcc tatcaaatca
     9781 aattcgatgg aaacagtgtc tatgccttga gttactatct ctatcagcga agcactatca
     9841 aatggttacc ggaagaacat gatattatcc aactaatcaa agaattagaa caacaagttt
     9901 ctcagtctta tccatctagt tatcactatg tccaacgaat tctggaatta agtaagatca
     9961 aattagatat tgaagacaca agtatggatc ggattttact gacgatctat ctaaaaaaag
    10021 cgaaatggtc aaaagaaaaa cgactgccaa aagcgatgat tgctgctcac ggttatgcaa
    10081 ctgctagcag tatggcaaat gtagtaaatc gtttattaca agaggatcta tttgaatcat
    10141 ttgatatgcc actagatgtc acaccacagc agattgcttc agaaatcctt gattattgcg
    10201 aaaacagcga tgtttcaaat ggattgatca tattagtaga catgggatct ctgaaagaaa
    10261 tccatcagtt ttttaaaaaa cagttatccg taccgctttt gattttgaat aatgtgacga
    10321 cacctttagc aatcacagta ggggaatgcc tgcaaaaaaa tgtatcatta gaagagatag
    10381 cagaagagac tgttcgacag atccagccag aatggcgact gctctatcca gaggaaaaca
    10441 aacccaaagc actcattact acttgcttca ctggaatcgg tacggcggtc cacttaagtg
    10501 aactgctaga aaaaagctta cctacggctt gtcagttgaa aatcatccca tatgaatatc
    10561 agcaattaaa agataagaaa aacagtgacc cattattttc catatacgaa gtcgtaggaa
    10621 tgattggcac cacagatcct gatatcacca atatcgacta tctttcatta gaagacctca
    10681 tctcggggga aaagatgtct gtactagctg aatggttgga cactactatg aatgcttcag
    10741 agaaggaaat attcaatcag cgcatcattc gtaatttttc attggaaaaa gtactggatt
    10801 ccgtgactat tctagataca gagaaggtga tgggagaaat cgatcatttt atgagagaat
    10861 tagaggtgca gttgaatcga agcatcagca atcccaaaaa attagcgttg tatgtccatg
    10921 tgagctgtct cattgaacga ttgatccgac aaatgccaat cgaaacctat caaggattag
    10981 agaaatggaa gcagtgtcaa aaagaagggt tatctgctat taaatctgcg tttagtgtca
    11041 ttgaagagca ttatagtgtc atgatcccca attctgagat tgcctatatc tatgatattt
    11101 tgagtgaaga tacagagttt ctctcgatag aagaagaatt ttgaacataa gcaagttgtt
    11161 gccactaatt ttggcatgca acttgctttt ttagtatgtg taagtgaaaa gaggagatga
    11221 aaaagtggat agagaaatta tcttagcttc ccatggaaaa tttgcttcag ggatattaga
    11281 ttcattagag ctgatctatg ggaaaaatca ttctattact gtattcgatt gctatactga
    11341 tgagaatttt gatttagcgg aaacagtaag gcagcttttt atgaggtaca aagataaaga
    11401 aatcattgtt ctgacggatc tttttggcgg aagtgtcaac aacgaattct tacaatatat
    11461 caatcgggat catgtctatc tcgttgccgg attgaatttg ccactattga ttgagctagt
    11521 atctcggttg gacgctgaag tagagacaac tgttttgatc cagcaggtgc taactgattc
    11581 aaaaaaaatg atccagtttt gtaacgagcg gctcaatcag aaaatagaag aagacgaatt
    11641 ttaggaggaa gaaaaatgat tgtattgaca agagtcgacc atcggttatt acacggacaa
    11701 gtagcttttt catggacaca aacaataggc gcagactgta ttttgattgc taatgatgat
    11761 gtacctacga atgagatacg aaagacaacg atcaaattag ccaagccaca aggagtcaaa
    11821 ctagtgatca aatcaattga tgactcgatt gccgcgttga aaagcggggt gacagataaa
    11881 tataagttat tcatcgtagt ggaatcgatc gaggatgcat ataagctggc agaagcgtat
    11941 ccagaaatca aaaccatcaa cttaggaggg atcaaggcaa aacctggtac tcgcagcatc
    12001 ggaaaagcgg taaatatttt accagaagag gaggaacgct tgaaacgatt aattgacaaa
    12061 caaatagaag tagaaatccg tcaagtacca gcagataaaa aagtggatgt aagagaagtt
    12121 ttgtaagaaa ggaagaacct caatgattgt acaagcagtc ttattaggaa ttgttgcatt
    12181 tgtcgcacaa tcagaatatg cactagggac atcgttgctt tctcgtccga ttgtgacggg
    12241 gctgtttaca ggaatcgtgt taggtgatgt aaaaactggg atcattatgg gcgcaacttt
    12301 agagttagca tttatcggat cgttctcagt aggtgcttcg attccacctg atgttgtgac
    12361 cggtgggatt ttagggacgg cttttgctat tacagctgga gccggtacag aaaccgcgct
    12421 tttactaggt ttgccgattg ctacgttgac attgatattg aaaaatattt acttagggct
    12481 tttgattcca attatgaacc ataaagcaga caattatgca gaagaaggaa attataaagg
    12541 agtcgagcat atgcacttgc ttgccggtat cggtctttcc ttgatgcttg gtgttgtggt
    12601 aatggtttct tatatggtgg gaagcaatac gatcggaaat ctattaaata tgattcctga
    12661 cttcgttcaa aaggggttgt cagtagctac agggttgatt ccagctcttg gttttgcgat
    12721 gcttgctcgt ttgctgatca acaaacaagt tgcaccatat tttttcttag gttttgctat
    12781 agcttcttac ttagagattc ctgtgaccgg agtagctatt tttggtgcga ttttagcagt
    12841 tgtggtagtc aatattatga atgtccgtca actaccactt caaacaactg caggggaggt
    12901 agaagaagat gaagaattct gagatcaaaa acagcaaaat aacgaaaaaa gagctaaatc
    12961 acgtattttg gcgttctttt caaatggagt tttcttggaa ttatgaacgt cagatgaata
    13021 tggcttatgt ttatgcaatg attccgattt tgaaaaagtt atatcagtcg aaagaagaaa
    13081 tggcagcagc tttgaaacgg catctagagt tttttaacac gaccccacat atcgttacac
    13141 tgattttagg aatcacggct gcaatggagg aagaaaataa ggatgatcct gaatttgatg
    13201 tgactgcgat cgatagtatc aaaacatctt tgatgggacc tcttgcaggg ttaggagatt
    13261 ctttcttttg gggaactttg cgattgattg ccacaggtgt gggtacctct ttagccatgc
    13321 aaggaaatat tttaggccct atcttgttca ttttgatttt caatattccg cacattctgt
    13381 ttcgctatct agcaacaggt tggggatatc gcttaggaac gggttttttg aaaaagatcc
    13441 aggcaaacgg tatgatggaa agtttgacat taggcgcgtc tattattgga ctgatggtcg
    13501 ttggagcaat gactgccacg atgatcacca tcaatattcc tttaaaaatc ggttcaggag
    13561 aaaatgctgt gactgttcaa agtatttttg atgatatcgt tccaaatatc ttatgtcttg
    13621 gagcatttgg catagtgttt tatctattga agaaagaagt gaaaccatta acgatcttga
    13681 tcggactagc tttctttgga atttttggtt cgtggatagg gattttttaa aaaaggagca
    13741 gagaaaatgt ttacgatgca agattatatc tatgaagaaa aggaagtact aagtacgatc
    13801 ttgaaaaaga atgatttttc gacaagagaa catatgaaaa aaacagtgaa tttactgatc
    13861 ttagcaacgg gttcatctta taacgcttgt ttagcagcga agccagcatt ggaatcttat
    13921 ggtgatctta ctgtcgatat tcaagaacct ttttattttt tcaattatgg aaaactttct
    13981 ccttcaatcg atactgtgat tgctgtgtca caaagcggga aaagtgcttc tactgttgaa
    14041 gcagtaaaaa tgatacaaaa acaagggtta cctgttgttg ctatcacaaa tgacgtgcaa
    14101 agtccacttg ctttagaagc tgcacaaatt attgatttag gtgccggggt agaatcagta
    14161 ggcttcgtta caaaaggcta ttctgcgact gttttacagt tactactctt agggatagga
    14221 attggaataa gtaaaaataa aatcagcaaa aaaatagaac aagactacat gcaacagttg
    14281 cgcaaaataa tcaaccattt accagcgatt attcaaaaaa cggaagaatt ttttgatgga
    14341 tatcaaagtt tatttcgttt agcacagcgt ttcgtcacca ttggatatgg tccaaactgg
    14401 ggaacagcta aagaagcaga gacgaaattg actgaaacaa tccgcgttcc gtcacaagga
    14461 tttgagttag aagcttatat gcacggccca tacctggagg cagatgcttc gcatttgcta
    14521 ttctttattg aaggggaaag tgtaaataag gaacgttcgc aaaagttgca aagatatatg
    14581 agtcggtacg ttggagagac tctcaccatt accacaaaaa aagcaagaaa cgagaaaaca
    14641 cttggattag caatagaatg tgatgaatat ctgtctgtat tagcgcttgt cgttccgttt
    14701 caactattcg cttataaaac cgcagtagct aaaggtattg acttgaacaa aaagattttt
    14761 gaagactttg atacagtctt gaaaagtaaa ttataagagg tggaaatcat gctgaaattc
    14821 aatgaagaag aacaaatcaa agcaataaaa ggcgcactag ctttacgtcc gcaagtagaa
    14881 gaaatcatcg ataaacttta tgcggaaaaa tttgacgctg tctattatct aggaatcggt
    14941 ggaacgtatg cttcctcgat gcaagctgtc acttatatga atgggaaaag caacttgcct
    15001 gtctttgtac agcatgctgc agaatattac actactggaa acaaaagatt aacaaaagat
    15061 tctatcgtta ttttgtcctc tgttaccggt acaactcaag aagtagtgaa agcagtagaa
    15121 caaatcaaga aagtaggagc gacactgatt ggatttatcg ataaagcgaa cagtaagttg
    15181 tctcagctat gcgattttgt ggtcacttat ccagctcctg gaacagaaca aatcaaattt
    15241 tttatggcag cagatcgatt gatgtatttg aatggagaat tcgaagctta ttcagaatac
    15301 tatgaacagc tcgaacgtta tttaccaaca gggttagtgg aagcagagaa aaaagcagat
    15361 gcatttggct tatcatttgc agaaaaacat cgacgtgata gtatgcatta ttttattgga
    15421 gcagggaatc aatggggagc tgtttattca tatgccatgt gttactggga agaacaaagc
    15481 tggcttccat cgaaatctat ccatgctgcc gagtttcttc acgggacgtt agagatcgta
    15541 gaagaaacaa ccccagtcac attattttta ggcgaagatg aacagagagt gttatctgaa
    15601 cgtgtggcta aacttttgcc aaaaatttgc agcaattata cattgataga cacaaaagac
    15661 tatccagtag aaggtatcag tgaaaaatat cgcggacgtg ttttgtcttt cttattgatg
    15721 catgtagtga cacaacgcat cgatgctcat gtggagcagt tgaactgcca tccattagag
    15781 atacgccgct actatcgtca gtttgattat taggatagac tggataaaaa gaatataaaa
    15841 aataaagaga gcagcgataa ctgctccctt tattttttta aaatattggc atcaattacg
    15901 ttataaaaca cattttttgc attatctaca gttcaactta taatctgttt aatgggacat
    15961 gtgggtgcca accatttttt gttatgcaat agtactattt agttgttgca tggatccgta
    16021 gtaaaagtcc aagtacaacg agttgcgcca gtttacatag cgttttgtat ccagaatgtt
    16081 gactaaaaca aaagttccta tgtcaaccgt caacagagac aatctgtccg ttgcaggacc
    16141 tgacagattt agaaatgttc aatctgtctc ttatgtattt taaatcaata ctcactagga
    16201 gaagtttcct aacctcctaa tagtaatgct ggtagtaaca ttgtcaaaac tgcttcctct
    16261 taggaacaag caaagcataa cgagtgtaaa aataaaatgt agactattgt aacatatcat
    16321 tgattatttc aagtaaaaac gataaaagaa gtaaacaaac acttaaaaac aatggaaatt
    16381 agtcccaact ttaagaaatg tatactataa tggaaattat caattattta tttggaggaa
    16441 tatccatgaa acaaaaaatc gcagttcttg gtcctggatc ttggggcact gctttagcac
    16501 aaacattggc agaaaacggg catgatgtcc gtatttgggg aaatgtacct gaacagattg
    16561 atgaaatcaa taagtatcat acaaatcagc attttttacc tgatttgacg atcccagaat
    16621 cgattatcgg atataaagaa ttggcagaag cagtggataa cgcagatgct attttatttg
    16681 ttgttccaac aaaagcgatt cgttcagtcg cacaggaact tattggtaaa atgaatacta
    16741 agccaatcat tatacatgca agtaaaggac tagaacaaga tacgcataaa cgaatctcag
    16801 aagttttggc tgaagaaatt ccagaagaaa aaagacaggc aattgttgta ttatctggtc
    16861 ctagccatgc tgaggaagtg gcagtacatg atatcacaac aattacagct gcaagtattg
    16921 atcaaaaagc agcagcctat gtacaaaaac tgttcatgaa tgattatttc aggatttata
    16981 caaataatga tgtgattggc gtagaaactg gtgcagcact gaaaaatatc attgcaattg
    17041 gtgccggcgc tatccatgga ttaggattcg gagatgatgc aaaagcagca atcatgactc
    17101 gaggattggc agaaatcagc cgtcttggtg tcgcgatggg tgccaatcct ctgacattta
    17161 tcggtttgag tggtgtgggg gacttgatcg ttacttgtac aagtgttcat tctcgcaatt
    17221 ggcgtgccgg taacttgtta ggacagggac ataagctgga agaagttcta gaaaacatgg
    17281 gaatggtcgt tgagggtgtg gcaacgacaa aagcagcagt agagctagct caacagctag
    17341 gtgttgagat gccaattact caaacaatct ataatgtttt gtataatgga gaagatatca
    17401 aaaaagcagc aaaagaaatc atgttacgtg acggaaaaat ggagaatgaa ttttctcttc
    17461 gttaaataga tgatcaatag ttaatcgtcg tttaggagga acagcaagtg aaggtcaaaa
    17521 aagcagtcat tcctgccgct gggctaggaa cacgtttttt gccggcaaca aaagccatgg
    17581 caaaagaaat gcttccaatc gtggataaac caacgatcca gtttattgtg gaagaagcat
    17641 tggattcggg aatcgaagac atcttgatcg taacgggaaa agaaaaaaga cccattgaag
    17701 atcactttga tgcaaatatc gaattggaaa acaatttgcg ggaaaaggaa aaaacagaat
    17761 tactcgcttt agttgaagaa acgactcaag tcaatcttca cttcatccgt caatctcatc
    17821 caagaggact gggggatgcc gtgcttcaag caaaagcatt tataggaaat gaaccgtttg
    17881 tcgtcatgct gggggatgat ttgatgaaag atgatatccc attgactcaa caattgatga
    17941 tggattatga agaaacacaa gcttccgctg ttgccgtcat gcgtgtacca gaaaatgaaa
    18001 catctaaata cggaattatt gatcctaaaa cagatagcga aaagggtcgt tgcagagtaa
    18061 aagggtttgt agaaaaacct gaaatgggaa aagcaccaag taatttagcc attatcggac
    18121 gttatctact gactcctaaa atatttgaga tattagaaac tcaagaacca ggagcaggaa
    18181 acgagataca gctaacggat gcacttcaga ctcttaacca gacagaggcg gtctatgcga
    18241 gagagtttaa aggaaaaaga tacgacgtag gcgataagtt aggatatatg aagacaaata
    18301 ttgaatatgg acttcaacat tcggaaattg ggacttcatt aagtgcatat attatccagt
    18361 tgagtaaaaa gctaagaaca gaaagttaac tggaaataag tggcctgctg agaatgaaag
    18421 gctatttgtt attaaagcgg cagcttgtgc gtcatttgcg caactgtcgt tttagttttt
    18481 tgactctaga tcagttgagt cgtggaaaac gaaagataga aaaagacttt cgtgaataga
    18541 ttcagaaaat attatgctaa tggtttaatt tttttttaag ttgtataaaa gaaaatgatt
    18601 acataagaga aatgaaagcc aagccagaag attcatagta gaataaaaag gcgaattttt
    18661 taattataaa aaaaggtgtg tgttcactat gggaaaatta gtcgctgttg cacagtcgct
    18721 gcttgatata ctcaagtggc tgatggaagg aagcaattgg atatttgtgt tgccactatt
    18781 gcttattagt ctatttatca tgtttcgcat ggtcagaaaa aaagaaacgt ttctgatttt
    18841 tatctggtat gcagcatccg ttggggttgc taccttgatg gttgaattgg cgttaggtag
    18901 ttgggaagct tgcgtatttg ctttattttt tgctgtactg tatctaatca tttatatccc
    18961 atatatgaaa agaaagtctc ggataggcag aaaaggtaga catacaacta ctttttctag
    19021 tacagattct ctcgaacaaa gtagaagaac gagaagaaga cggtaatatt tagtaggtat
    19081 acgcaaaaaa agataaaata atggataaac tattgagatg agagatttat ttaattggtg
    19141 gaaaaaagct tttttcactt atataaaaga atctttttta aaggaatgct tgttgtatcg
    19201 ggataagcca ttcctttttt tatgttattt atatatatat aaatcatacc aatgtttttt
    19261 tgtattttct tgcttgcact tggataagag attacttaca ataaattcag atagaaacag
    19321 ctgaataaaa aaaggctccc tgacccttag ttggcgctaa gaatcaggga atgaccagtc
    19381 acacacatac accctgaccg atcattttgc catagtcaat gcagtgcatc gacctaattt
    19441 attgtaccta atctagggtg aaaattctac ccctatgtag atattttcca tggtctgcac
    19501 gttgttttat ttgctatgtc aaggtattgg gcgtattcgt tcaatgcctt ttttgtgttt
    19561 gaaacagcaa agaccacatg tgttactgtc taactactgt ttttttatgt acttccttag
    19621 attaggcagc tgtttctatt catgtcaagt atagctaggc gggagagata aatgagttta
    19681 tgttattttg actattatca ccaattggaa gagaaattaa ggatagaaga gccgctgatg
    19741 cttcaagctg gcacttggat cgcgaaacaa attaatcaag gtggtagatt gcagatatat
    19801 gctagccgca cactaagtgg tgtagcattt gaattttggg atcaaacccc ggatcttgta
    19861 ccaagtatat tgattgaaaa tccagccgga ggtgtctatg aaacgctaga aggtgcagga
    19921 caggcaatta ttgatcagct ttctgtcaaa tcagaagaca tttttcttct tctttcgaat
    19981 gaaggaagaa acccggcaat catcgaacta gctcagtgga tcaaagaaaa tggtcatcca
    20041 ttaatcattg ttactggatt tgatttgtca cgaagtatca aatcgcaaca tagcagtggt
    20101 ttgcgattat ttgaatttgc tgatttggtt ttggacaacc acgccaattt acatgatgca
    20161 gtcttgactt tgccaaatct agatccagcg atctgcggaa cagcctctct tgcaacgatc
    20221 tttttgctgc agcaagtttt gtattttaca gcaaaacagc tagtccagga cacaagtgga
    20281 aaatagttat ccacatgttg acaaagttcc ttaaaaaaat aatgttccac ataaaaatgg
    20341 agcgtttgaa aaaaagaaaa cgattattaa tgaggattaa caacgactac tttttttgtt
    20401 ttagttattt taagatggat catctttgat tttaagcttg ttttgtttaa agtttgttat
    20461 ttttataagt tgtttcacag ttatgagaaa tgagttataa gaatatgtag tgtcagaaaa
    20521 ataaaaagta gtggaaaaag agacaagaaa gaatccatat ggattctttc ttgtctctta
    20581 atacacgttg atgaaagagt cttccccaaa actctttaca aagtaattat agtacatttg
    20641 ttatcattat tgcaatgaag tttacctgat ataacaaaat tgtccaacag gatacctttt
    20701 tctaatagaa ataaaataca caaaaattga aggatagata atggatattt ataagtgttt
    20761 attatttttt ttgcaatgaa attcaatttt tcaatgattg taaaatgaaa aaaacaggac
    20821 gagattttct ctaatctaaa aataaaaaaa gaattttaag gaaaaacaga gaaataaaat
    20881 acttaatata aatggaaaga gaaaaaaata atcatacata ggagaaaaaa aacaaaatag
    20941 aaaaaagtga ttttctaata aattattcat gtggaaatgt catttttact gtataattca
    21001 tttgattatc cattttttat tcatcaaaat tactgaaaaa agaaaggaag aaataaattt
    21061 gaagaaaaca aattcttcag gaaaaccgaa acgcgacttc actaaattat cgacacccca
    21121 tacgtatgtc attatttttg gtgtagtcat ctttgcatgg atactgactt ttgtcgttcc
    21181 agcaggaaag ttcagcacgc aagatattga atacaaagat gcaaatggcg aaacaagtac
    21241 tcgtactgtt ttacgtcaag attccttccg ttatgcttac gagctggata agtcgtatgt
    21301 gtttgatcaa ttagaagaac tgcaagatca tccagcagaa cgtgaaaaac tggatgttcc
    21361 ggaaaaagga ttggaaaaag tcattgctga cggagaaaaa aatcttacgc aggaaaaact
    21421 agatgaaatc tccttgacag atgatgtgct ttacgatcag tacggcgaaa atatctatga
    21481 tacttctaaa aaattgcata aaacagccaa aatatgggga acagatgatt ttggcggctt
    21541 tggtttctta aattttgttt ttgaaggact agtatctgga gataagtatg gatctgctgt
    21601 agggatcgct gcattgatcc ttgttgttgg aggtgctttt ggtattatta tgcgtaccgg
    21661 agcgatcgac gcaggtatct atgcgtttat cagtaaaact aagggactgg agcgtttagc
    21721 tttaccgcta ttattctttg ctttttcatt cggtggcgct acattcggga tggcagaaga
    21781 ggtaattcca ttctcaatgg tgatggttcc atttgtcatt gcgttaggat atgactcgat
    21841 cgtagcagta accgtgactt atgtcgcatc tcaagtcgga aatgcgacct cttggatgag
    21901 cccgtttagc gtagcggtgg ctcaagggat tgctggaatc cccgtgttgt ctggtgcgac
    21961 attccgattg attatgtggg tagttgttac tgcattagcc gctggttata tgatgatata
    22021 tgcagaaaaa atccgtaaaa aaccagaaag ttcgttgact tacaaatcag atgattattt
    22081 ccgttctcat atcaaaaaga catctgatga aaataaaaaa ttcatgcttg ggcataaatt
    22141 gatcttatta gagatgttag ttgtccttgt ctggatcgtt tggggcgtga ctcaaaaagg
    22201 ctattacatt ccagaaatcg cttctcaatt ctttgtgatg ggattagttg ctggaatcat
    22261 tgctgttctt ttcaaattag atggaatgaa agtcaatgac atcgcttctt ctttccaatc
    22321 tggtgcagct gatttagctg gtacagcaat cgtagtaggt atggccaaag ggattcttct
    22381 tgtcttggga ggatctgatg catctgttcc ttcagctttg aatacgattt tgcatgggat
    22441 cgggacggcg ttgaccggtg ttccagctgt cattggtgct tgggcgatgt acatcttcca
    22501 aagtttgttc aacttggttg ttacatcaaa ttcaggacaa gcagctttga ctatgccaat
    22561 catggctcca ctggcagatt tagtaggtgt atccagacaa gtagcagttc ttgcttatca
    22621 gcttggttca ggttttatgg acgcttttac acccgtatca gccagcttga ttggtgtgct
    22681 aggtgttgca cggatcgaat gggcaaaatg ggcaagattc caaatcaaga tgcaaggatt
    22741 cctgtttgtt ttaggaacta ttttcatcat gattgccatt gcaataggtc tgcagtagta
    22801 ctggagacct gtaaataaaa aagtaatcca aacagtagat aaggactcca agaaccgtca
    22861 ttttacggta aaatcaaagg agtaaagatc ctatcgtttg gattattttt taagatccta
    22921 tcatcagtta tacttaaaga aagaggaaaa atgtatgaaa atcattcgac aaattgaagt
    22981 gtttgcacca gattacttag gaaaaatgga tgtactgatt gccggtggga agattttggc
    23041 tgtagaagaa cagctagaag gcggctacga aggagtggaa gtagaagaac tttcaggtga
    23101 gggaaaagta ctaaccccgg gtttcattga ctgtcatttc catattttag gtggaggagg
    23161 agaaggcggt tatcaaaatc gaacgccaga agtcacattg agccaattga ctacagctgg
    23221 tgtaacgaca gttgtcggtt gtttgggaac agacggagaa ggtagagata tgactgcgtt
    23281 gatcagcaaa gcgaaaggtc tggaagcaga aggaatctcc acttatgtat acgaaggttc
    23341 gtaccgattg ccggtaaaaa cagtaacaga ttctatcatc aaagactttt tgacaattga
    23401 taaaattatc ggaatcggag aaatcgcagt atccgatcac cgttcttctc agccaagttt
    23461 tgaagagttc gctcgtgcag tagcagatgc tcgtgtagga ggcatgttat ccggaaaagc
    23521 tgggatcatc aatgtccact taggcggagg aaaaagaaag ctagagctat tgacacgaat
    23581 cgtagaagaa acagaaattc cgatcacaca atttttgcct actcatgcaa atcgcacacc
    23641 agaattattt gaagcttgcg tggcatttgc caaacgtggt ggcacgattg acttcactgc
    23701 tagcgaagat cctgattttt gggagaagac agatggtgaa gttcgcttca gcaaagcact
    23761 gaaacgattg atcgaggaag atgtttcttt agataatttc acgatgacct cagatggaca
    23821 aggaagcttg ccttattttg atgaaaacaa tcatttccta gggttaggag taggaagtgc
    23881 caaggcattg ctagtaggga tcaaagaagc tgtgcaaaaa gaaagtatcc ctttggaaat
    23941 cgccttacga gctataacca gtaatccagc acgtatattg aaattggata aaaaaggaaa
    24001 aatcgaaatt ggtgctgatg cagatctgtg tatcttggat aaagaaacgc ttgatattga
    24061 tacagttatc gctaaaggag aaataatggt ccaagaaaaa gaagtcaaag tatgggggac
    24121 tttcgaaaaa agtttttaat caacagctct aactatcttt tttagaaaac gagcgttctg
    24181 tttgtctgta tctgttgttg atgttaccct gaaggtagat caataaacaa actaagagga
    24241 ggaagcacaa tggcagattt aaaaggacgt tctaaagata tgaaagataa agtagtcggt
    24301 gaaggaaaag aagcatacgg caaagcaaca gacgacaaga gcaaagaaac agaagggaaa
    24361 gcacaatcac taggtgctga catcaaagaa aaagctcgaa atttaggcga cgaagtcaaa
    24421 gacaagtttt ctcgaaaaga cgaaaaaaaa taaaaaccac ttattactaa ttgaatcaag
    24481 caaataaaaa atccgaacca tatctgcgag acagatatgg ttcggatttt ttatttgatc
    24541 attatcctgt tttttaaact tcgatttttt gaaatttttt attttttagt tcaaagactt
    24601 catcacagct ttttgctact tcaatctcat gagtcacgat gataatacag cgattttgtt
    24661 catgggcgat ttcttggaaa agtttgacga tatcttgtga agttgtttca tctagatttc
    24721 cagtaggctc atccgctacg attagttgat gttcacagca gatagcacga acgatagcga
    24781 ctcgctgctg ctgacctccc gaaagtttgg tcacctgttt ttttgctaat atttcatcaa
    24841 ttcctacacg agccagtcga tctaaagcaa cttgtcgata attctcttgc ttggtcttag
    24901 tgatttccat tgctgctatg acgttgtcta gagccgataa ataaggaagc aagttgtaag
    24961 cttggaaaat aatcgagaca tcatttttac gataacttct taacccgatt ttgttcagtg
    25021 aacgatcttg atagtatacc tttccttctt ttggtgcatc tagtccagaa ataagcgaaa
    25081 gaaaagtcgt ttttccagaa ccacttgctc ctaaaatagc gtacattttt ccagcttcga
    25141 aattcagttc tacatcttta taaagcgcat cttgcggatc ttgataccaa aatcccaatt
    25201 gttctgtccg taacatcaaa ttcctccttt agtttaatag gatttttctt gggtttaatc
    25261 ttaatatccc tatagaagct aaaaagacag aaaacagact gattccaaat gctactccgg
    25321 ctaatagtcc caattgtgca ggtttgacat tgatttccaa ttcgctgact tgatcagaaa
    25381 cagcaaatgg attattcgtg gttccttgtt gaccatcgcg ttgaccgcct tgttcttgtc
    25441 tgttacctgg catttgtcca ccgccaggag cttgctggtt ttgtgtcgtg gtttctgtct
    25501 gttggtcaag tagctgctgt cccacggcat tcgcgacgac gtttccgcta agcgaagcaa
    25561 ctcctaatgc aagaatcata caaatagcca cttcggtaaa caattggaga atgattttac
    25621 ttctggattc tcctaaagat agcaagacgc ctaactcatg ccgacgctcc cgaatggtaa
    25681 tcataatgat cagagtaagt atgataatac cggctacagc cactagtaaa acaacatttt
    25741 tagaaaaact tgccacgttg tttaaaggtt ccaacattga ctgatacatg gaatcatttg
    25801 attgtaagct aaatgtatct gtgtcgatca gcttctcagc ttctttgaga aactcatcca
    25861 ttttatctgg atcagataga gtgtaaatag cagaatctat cgtgttatcg tcagatgtcc
    25921 catttagttc atttgcaaaa gtgtaagaag tgtaaagtgt attcgacgga ttcatgaagt
    25981 tgaatttcat acccatactg ccgcttgttt cgctggattc ataaattcct ttgatcgtca
    26041 tttcataagt ggtatcttca tcttcagtac tcgtgattgt gaaagtatct ccgactgcca
    26101 ggtcatttgt ttctgctaga gtagaatcaa tgactacatt gtttgtgcct ttgtcgctgt
    26161 catctattcc ttcaccatcc gtgatttttg ctgttccatc agaaaaatta ctattttgtg
    26221 cagttgctga cacaccactg atttgaaagt ctgcttgagt catttgcgga ttcccgccca
    26281 ttcctccagc aggttcaccg cctgcatcag tagtttctga actgtcttca cttgaatcag
    26341 aactagtgat ggcagttatt ccatcttttg ccatagctga ggcggaactt tcaaaggaat
    26401 aactctttac attatctaat tcagctattt tttccgcgtc cgttaaggaa actggtgtaa
    26461 gagaaaaact gcctggatct ggacgttctt cagtggtaga accgcttgaa gaagtttgtt
    26521 cctgtttttt aaatgaagct tctcgattgg cacttaaagt cactgttgct ccaacttctt
    26581 tttgtgcttg attcgctgct tcttctgcag cgcttcgaat cgtgaggcca gctagcacaa
    26641 aaataagaat ggctgtgaac acggcaaaca ataacaatga acgtccccat tttacttttg
    26701 tacttttcca tgcacgtttc ataaaattca tattttgcat ccctcctagt agatgcattt
    26761 gataatagac aacctatctt aaagctagct taaatcttat gctgtttgca caccatcaac
    26821 atgaggttct gtatatataa aaagtcacta tagttattgc aaaaaatgtg gtaaaatgaa
    26881 aaaagtatgt ttcaaaccga cgaagaagaa atgaaagagg aatagcaaaa gaaatcctag
    26941 atgctacaga gggattttta cgtgcatagt tgttctaaca attgctgata ctagcgattt
    27001 agtttatttg ttgtataata atggggaaca gctgaatact tatataagta ttctggctat
    27061 agcgtttcgc aaaagatcca ttttgttaat gaagtatatc caaaataaaa tgcccatcca
    27121 actgtcagaa cgcggatgtg ctggtaattt tcctgctgat tctccataag ttgagaaata
    27181 gtgattttgc atatgcttca ttggaatggc tcatatattt agataaagaa gaaaatcgac
    27241 atctttaaac ttggatgtca taagaagagg tgaagtttgt gagtaccatt aaaattatcc
    27301 cattaggcgg cgttcgtgaa aatggaaaaa acatgtatgt cgcagaagtg ggcgaggata
    27361 tttttatttt agattgtggg ctaaaatatc ctgaaaatga gttgttgggg atcgacgtcg
    27421 taatccctga ttttacgtat ttagaagaaa acattgatcg tgtagctggt gtctttttaa
    27481 cacatggaca tgcagatgcg atcggggcac ttccttactt gttgtcaaaa atttctgtac
    27541 cagtattcgg gacagagttg acgatcgagt tagcaaaatt gaatgtcagt cgtaatgaag
    27601 cagccaaagg atttaaggat ttccatgtgg tagatgagca tacagaaata gactttggtg
    27661 aaacagtagt gagctttttc cgtaccactc atacgatacc agattcagtg gggatcaatc
    27721 tgaaaacaaa agaaggtagt atcgtttata cgggagattt taaatttgac caaacggcta
    27781 tcccaatgta tcaaacagat tatgcacgat tggcggaaat cggcaaagaa ggagtcttag
    27841 ccttactaag tgaatcggca aatgcagaaa atcctcagcc tgtagcttct gaagcccaaa
    27901 tagcagatga agtatttgat acgatcaaat attgggaagg gcgtatcatc gtcgcttgtg
    27961 tagcaagtaa tttgcaacga gtgcaacaag tattggatgc agcagaaaaa gcacaacgaa
    28021 aagttgtctt gactggtcaa gatttcgaac gaattattcg cacagcgatg cgtttagaaa
    28081 aacttcaatt accaagtgaa gatctgcttg ttacgttgaa agacatgaaa aactatgatc
    28141 cagaagaact gatcatttta gaaactggcc gaatgggtga acccatcaag tctctgcaaa
    28201 aaatggcaaa cggtacacac cgtaatgtca agattgaaga aggagatttg gtttatatca
    28261 ctactacgcc aagcatcgcg atggaaacca tcgtggcgaa aacggaagat atcatttatc
    28321 gagctggtgg aacagtgaag ttgatctctg agaatatgcg cgtttctggc catgccaatc
    28381 ctaatgactt gcaattaatg atcaatttga tgaaaccaac atattttgtg ccagttcaag
    28441 gagagtatcg tgaattagct gctcatgcag atttggctca tgcagtaggg atgccataca
    28501 aaaatatcta tattactgga cgcggagacg tgctggaata taagaacaag cgtatgagtg
    28561 ttgcaggaac tacaagtgca gaaaatgtaa tgattgatgg attaggcata ggtgatatcg
    28621 gaaacattgt tttacgtgat cgaaaaatct tgtcagaaga cgggattttc gtagcggttg
    28681 tcactatcag tcggcgagaa aagaaaatca tttcaaaacc tcaaattact tcacggggat
    28741 tcgtttacgt aaaagctagc cgtgatctga tgaaagaaag cagcaatatg attgaaacaa
    28801 tcgttgagaa acatttagaa agcaatgatt ttgagtggag caaattaaag caagacatcc
    28861 gagatcaatt aggccgttac ttatttgaac aaacaaaacg ccgcccagtg atcttgccag
    28921 tcattatgga agctactcaa cgtcgaaata aaaaataaac aaaaagagta agatgaacct
    28981 ctctatagag tgctcgtctt gctctttttt tgcgaaaaac aaataatttt tgaatacagc
    29041 atccctcgct ttataataaa cgatagaata aagggagtga atcaatgatg agtcaaacaa
    29101 aagtcgaacg ttatttagaa gaacaaaaga tcagctatca accacttgat tttagtcatg
    29161 tggtagaaga atctttcaca gaagaactac aaaaaagagg gattgatcca gctttgatat
    29221 gtaaaactct cgtagtgaaa ggaaacaaaa ctggggtgat catcgcattg attcctttga
    29281 acgatcactt agattacaaa aaaacgagac aagtgacgaa agatcgtaaa gttggttttc
    29341 ctggaatgga ttttgtatta atgcatacag gatatcccca cggagcaaac acccctattg
    29401 gaataaaact cgcacatcct gattattgtt ttttagcaga cagttcttta aaagaaaaag
    29461 aagaagtaat tgtgtcaagc ggtgaaatcg ggagaagcct tcggataaaa gtagccgatt
    29521 tagaaaagat cgtccatcct atttacgctg atttaattag ttaagggacg agaagagaag
    29581 ctaagcagag cagagacctc aaacttgaga ttatatctgc ttggtacttg gtgcatcctg
    29641 tattatctgt tacaattgct ttgtacatac tgtactatca atccaatgaa aatatattta
    29701 gcagctatat tttttcattg atgtgcaagt gatgaaatcg tttttaataa aaaagataca
    29761 gagaaaacgc gaggtgtaaa atatgaaaat tacgtatcat ggacattcct gtttaacaat
    29821 tgaaatggaa aatggccaac gcgtgatgat tgacccgttt attacaggaa atccattgac
    29881 agacttaact gcagaagaag taaagacaga ttggttgctg attactcatg gtcatagtga
    29941 ccacatagga gatatgattc ctattgcaaa agaaaatcaa gccacaatta tcagtattgt
    30001 tgagattgct gattatgcga acagcagagg agtagattca ttcggtatga atatcggtgg
    30061 aaaacatgcg tttccattcg gcgtggtaaa attcgtacat gcacagcata gttcaagcta
    30121 tgaagtagac ggtatcgttc agtatatggg cgagcctagc ggaattatta ttcaagcaga
    30181 aggaaaaacg atttatcatg caggggatac tgcttatttc agtgatctag gattgcttgc
    30241 agaagaattc gatattgatg tcgctttctt gccgattggc gataactata cgatggggcc
    30301 agaagatgca gcaaaggctg cgcaaacaat tcgtgccaag aaagtagttc caatccatta
    30361 caatactttc cctgtcattg aacaagatcc cgaatctttt gtagagttac tggaagatag
    30421 tgaaggaaaa ataatgagcg tctcggaaac gctggaagtg taggcagaaa atggcgacaa
    30481 aacatgattt gattttagaa tatattgaaa gtttacctgt tggcaatcga atttctgtaa
    30541 gaagcatcgc aaaagaattg aatgtcagtg aaggaacagc gtaccgtgca atcaaagatg
    30601 cagaaaatgt cggactagtt tcaacaattc aacgagtagg gaccatccgt atcgaacgca
    30661 aattaaaaaa acatattgaa cgtttgacat ttggcgaggt cgtcaggatc attgaagggg
    30721 acgttttagg cggggcggca ggattagaca aggtcttgaa taaattcgtc atcggcgcga
    30781 tgactaaaca tgccatggaa cgctatatta cgccaggatc actaatgatc gtcggcaacc
    30841 gggcggaagt ccaaaaatta gcattggaag atggcgccgc agtattgatc acaggaggat
    30901 tcaatacgac tgctgagatt gcaaagctag ctgatgatct tgaaatgccg atcttacgca
    30961 cctcttatga tacatttaca gtcgcaacca tgatcaaccg agcgttaagc gaccaattga
    31021 tcaaaaaaga tatcatgctt gtcagtgata tctatatgcc agctgaaaag actcattatt
    31081 tgcagcagac agatacgatt tctgattatc agcgtttgtc agaagaaacg cagcattcgc
    31141 gttttccagt cgtcaaccgc catcatcgtt tgatggggat tgttacggca aaagatgttt
    31201 tagggaaatc gccaaaccag atcatcgatc gggtgatgac gaaagaacca atcagtgtaa
    31261 agaaaacaat gagtattgcc tctgttagcc atcaaatgat ctgggatggg ctagaagtca
    31321 tgccggtcgt ttccgatgac ttgacattgg aaggcttgat tacccggcaa gatgtaatga
    31381 aagcgatgca gcttgtccag cgacaaccgc agattgcaga tacgatttcg gatcagatat
    31441 ctggaaatat ccatacgatt gatactgacc gtgaaggaaa tcgtttggaa aaccctaagt
    31501 tcaaattcgt agtagcacca caaatggtca atggcgttgg aaccatttct tttggcgtac
    31561 ttagcgaaat catttctgat gtcgcacaaa aaacaatggt gatgaaccaa aaacgaaata
    31621 tcttgattga acaagtgaac ctacattatt tgcggctgat ccaattggaa agcgaattag
    31681 acattcgtcc acgtgtattg gaaatcggca gacgttccgc taaactggat atcgaagtgt
    31741 ttattgaaaa caccatcgtt tccaaagcaa tcgttgtttg tcaagtaatg gaacgttctt
    31801 agaaagcagg agtgaaatga cagtacagca agaaatagta gatacaataa aaaaatacga
    31861 taaaatcatt atccacaggc atttgcgccc tgatcctgat gcattaggat cacaagtagg
    31921 attagccgaa ctattacggg aaagtttccc agaaaaagaa atacgtcaag taggtgaaac
    31981 gattgaaggg cttcgctttt tagcagaaat gcaagaagtt gaagatgtct tctaccaagg
    32041 agcgctagtc atcgtgactg atacagctaa ttcacccaga atcagtgatc aaagatatca
    32101 gttggcagaa aaactgatca aaatcgacca tcatcccaat gatgaccctt atggagatct
    32161 tgtgtgggta aatacaaaag caagtagctg tagtgagatg attgcagaat ttgctttgat
    32221 gtttcctgaa tctttaaaga tgaacgttaa ggctgcacgg ttactttatg caggaatcgt
    32281 cggagataca gggcgctttt tgtatccctc cacgacgtcc cgtacattag agattgcttc
    32341 gatattgaga aattatccat ttgatgcaag tgctttgaac agagagattg aacaaatgcc
    32401 gatgaaagtc gctaaacttt ccggttatct ttatcaaaat atccaggtgg atgaaaatgg
    32461 tgcaggaaga ataatccttc cgcaagaaat tcttgataaa tatggaatcg atgattcaga
    32521 gacagcttcg atcgtttcac ttcctggagt gattgacgaa gtgctggctt gggggatttt
    32581 tgttcagcag ccttcagggt attaccgcgt acgtttacgt tctaaggggc cgatcatcaa
    32641 tgaattggct aaacgccatc atggaggagg ccatccatta gcaagtggtg cgaatgcaaa
    32701 agatttagca gaagtagagg aaatctataa agagatccaa gatatatgcc aatcgtataa
    32761 gagcagaaaa acagaatgat acttcgttcg aaaacgtgcc ttttgtagta aaaatcacaa
    32821 atgtatcttt tcaagatctt ctttttccgt tacaatagtg ctcactaaag attttaaagg
    32881 agaaagaaga tgacattacc aaattgccca gaatgtggct cagagtacac atatgaagat
    32941 cgaggcttgt tgatttgccc agaatgcggg aacgaatgga acaaagaaga acaagaaact
    33001 gtcatagaag acggattagt ggtattagat gcaaatggaa accgtctgtt agagggcgat
    33061 agcgtgacag tgattaaaga cttgaaagta aaaggtgcgt caggtgccat taaacaagga
    33121 acaaaagtca agaacatccg tttagtagaa ggcgatcata atatcgattg caaggtagaa
    33181 ggatttggac cgatgaaact gaaatcggaa tttgtgaaaa agaattaaag aatattcaag
    33241 gtcttttcac attcttttcc tactactaat gataaaataa agtaaacgaa aaggagtgaa
    33301 acgaaaatgt atggttatgg aatgtatggt tttttagatc cgacatttct tttggtcatt
    33361 cttggattag ttatttccgg aattgcgtca gcgtacgtca atagtacttt ccgaaaatac
    33421 gatcaagtga gaagcaaaaa caatgtgact ggtacacaag cagcacaata tatattacaa
    33481 agtcaacaga taaataatgt cggtgttcaa caaattgccg gagatctcac agataactac
    33541 aattcaggta ataagatgtt gagtttgtct caagcaactg cacagtctac ttcagttgct
    33601 gcgataggtg tagcagctca tgaatgcgga catgcggtac aagatgcttc gggttatgtc
    33661 ccattgaaga ttcgtgcagc acttgttcca gcagctaact ttggttcgat gatttcgttc
    33721 cccttgatta tggtaggtgt attgcttagc tggaactcta cgttaatcaa tatcgggatt
    33781 ttcgcttttt ctttagcatt acttttccaa ttggtgacat taccagtcga atttaacgcg
    33841 tctcggcgag caatccaaat tttgagtgaa ggcggactac tgacggaaga agaagtaccg
    33901 atggcaagac acgtattatt tgcagcagca ctgacttatg ttgcagcagc gctttcgaca
    33961 ttcctgcagc tgttgcgctt gatcttattg ttcggcggca accgaagaga ttaaaataaa
    34021 gaagttgtga aatcagcgct ttgatctgag ctaataccga gagttagctg attgaatacc
    34081 atttatttgg aataagagga tgtgacaaga attttgtcac atcctctttg ttttgttctc
    34141 ataacctttt ttcatataca gttcatgaac cgcttttatt aacttgccaa ataacactgt
    34201 attgtgcgtt gtatccagat agaaagtctc cgtttcatct gaaattttct catgctgatc
    34261 tgtaatgatc aaatcagcat gttctgaata tgcggtaatt ttaacagcat ctttgcgaaa
    34321 taactgctgc aaatggcttt gaagatgata atttcctgta aactgtttgg ttatctgcaa
    34381 atggatgagt aactgctctt tcgtttctgt ttgtagtaag tgttggatca attgactaag
    34441 gtattgactg atttggtttc tttccccatt ttttgaaaaa attgtatcga ttttttctct
    34501 taatttcttt tcctgtataa aagaactaga atgattggac gtggcttctg gaaagcaaag
    34561 tgtagaaaaa ctactgctgg attcacccaa cagttcgaaa aaaacaactg ccaacacaag
    34621 aaaataaaaa taaattggta ttttattttc taaatggttg agaggaaact gttgagttaa
    34681 gtaagtcatc aattgaccga cttgtcgaca aataaggttt ttgcttgtgg aaaataaaaa
    34741 accaatctcc tgtttttgtc gaaaagagat ttgatttgga aaaaaacaat agaaaaaata
    34801 attaaagtag gctctctctg tttctgtctc ttcacaaggg cgactcactg aaaaagaagt
    34861 aaagtattct gaataatcat gtgtatgctg caataagtcc atcaattcag tgatttcaga
    34921 agaagttttt ggaaaatatg cctgttgttt gtttcgtaat atgaataaag ttaacagcaa
    34981 gtagatattt ttttcttgtt ttgaaatttt ttctatttgc tcatctgtac tgttcatttt
    35041 tagtgcattt aattcaagtg aggaaaatgg ccaatcgtta cctccaaatg tatctgaaag
    35101 aaaaaagtaa agatagagac ggatagataa ctcatttcct tcaagagtac agcaattttt
    35161 tacagatagg atccgtaaat ggaattttat taaggaatga ttgagctgtt ttgtcagttt
    35221 ttcgagatag gagggcgata tataaagttg ttcaagtaaa tatttttttg gtacagcagt
    35281 ttgtggataa ttgattagta acatcaaata gttaaaacga ttggattctt ttaggtagca
    35341 taattttaac ttagaaaaaa cagctaactt tttagtttga tcggtttctt tggtttggat
    35401 ctctccttta tgattagaat aaataaattc atcaatcaat aaaaattgct tgagtgatac
    35461 atttattttt cggatcagtt ctttgattgt ccgttttgga atatgtaagt cattggataa
    35521 ttcttgtaaa gaacaattat ttttttgaat gaccatttgt agaacaagta actctttttt
    35581 tcttgcttca attagcataa atacctcctt tttattctgt ttttgtatat agttagacaa
    35641 aaactgtgag tggaaagtca tttcagtctg tgttattctc acagagtgtc gccttctttt
    35701 ttatataacg ttatttaatt tttttgttac cttgtttatt ctattgttta tttcctaggg
    35761 tattttgtca agatattctt gtttttaagg cggaaaaatt gatttttaat ggatataact
    35821 aaatatgttt tgataattat caataatatt tttagaatta tttatgttat ttatttatgg
    35881 gtagatgaaa agagacggag gtaagaatga tggggagagt aacaaattat agtaaagaat
    35941 atttgctgtt caaaagtatg gtatacgtgg aagaatatgg aatgaagtca attactgcaa
    36001 gagagttagc agagttctgc ggttgttcta catacccgat ttatacacat ttcaaatcaa
    36061 tcagcgggtt aaaagagaag atattagaag aaatcactgt ttattttgaa aactacttag
    36121 ctgaatgcag ttccgatgat gttttaagca ttgtctactt attgaaagat tttttttcta
    36181 tccatgaaag tattcgtgaa atcattgcaa aatctgattt agacatggat tcgctattta
    36241 agcggccatt tttctcatat gtcaaaggtc acacgaagat caaagaagaa aagtcgcttg
    36301 aattggtatg gcttaatgca ttgggaagtt tgcgctcaga tgattcttct caagattttc
    36361 ttgaaatgat gaacgctgtt tgggataaaa acgaggtgac ggatgaatac ttggaaaata
    36421 tcttaaagga acagtcacct taggcagcaa catgaaaaag tagatcagcc acctttattt
    36481 ttcgtaaaaa agattgtaaa atgtctaacg aaacattgga atgaaggagg aagcgacaac
    36541 gttaccacag caatttatca aaaaatatcg tcttctttta ggcgaagaag catccgattt
    36601 tttttctgcg cttgaacaag gaagcgtgaa aaaaggattt cggtggaatc cactaaaacc
    36661 ggcaggattg gacatggtcc aaacttacca tagcgaagaa ttacaacccg ctccctacag
    36721 caatgaggga tttcttggca ctgtcaatgg aaagtctttc ctccatcaag ctgggtatga
    36781 atatagccaa gaaccaagtg caatgatcgt tggaacagct gctgcagcaa aacccggtga
    36841 aaaagttttg gatttgtgtg cggctcccgg tggtaaatca acccaattag ctgctcaaat
    36901 gaaggggaaa gggttattag tgacgaatga gatctttccg aaacgtgcga agattttatc
    36961 agaaaatata gaacgatggg gagtttcgaa cgcaatagtg acaaatcatg cacccgcaga
    37021 acttgtccct catttttcgg gcttttttga tcggatcgtg gttgatgctc cttgttcagg
    37081 agaaggaatg ttccgaaaag atccaaatgc aataaaagag tggacagaag aatctccgct
    37141 atactgtcaa aaacgtcagc aagagatttt gtcttcagca ataaaaatgc taaaaaataa
    37201 aggacaactg atttattcga cttgtacgtt tgcaccagaa gaaaatgaag agatcattag
    37261 ctggcttgta gaaaattatc cagtaacgat tgaggagatt cctttgactc aatccgttag
    37321 ttcaggacgt tctgaatggg gaagcgtagc tggtttggaa aaaacaatac gtatatggcc
    37381 tcataaagat cagggtgaag gacattttgt tgcaaagctg acttttcatg gacaaaatca
    37441 gatgcataaa gaaaagaaga cacgaaaaaa aagtaaagtg caaatgacaa aagagcagga
    37501 aaaactctgg acggagtttt caaatgattt tcattatgaa gctactggaa gacttttagt
    37561 gttcaacgac catctttggg aagttcctga gttagcccct tctctcgatg gtttaaaagt
    37621 cgtacgtaca ggtttgcatt taggagattt caaaaagaat cgatttgaac ctagctatgc
    37681 attagcgctt gctacaaaaa agatagagaa tataccttgc ttgccgatca ctcaaaagga
    37741 atggcaatct tacacagcag gagaaacatt tcaacgtgat gggaatcaag gatgggtttt
    37801 gctagtatta gataagatac ctgtcggatt tgggaaacaa gtaaaaggaa cagtaaagaa
    37861 ctttttccct aaaggattga gatttcactg agtggattag ttcgtttatt tttttgtcta
    37921 tgctatgatg gagatagata aaaaggagct gatacttatg aaaacatcaa caaaagtaac
    37981 aatcggatta agcgtagcag ccgctgccag tgtagcgaca gcagtcgttg tatcgggaaa
    38041 agtaatggaa aagatccatc atatgactac tcggacgaaa gtaaaaaaat tcgtctatga
    38101 caaatttgat ggaaatgaaa aattactaga aatcgtagat ggtctttcag atagtgactt
    38161 ggattctttg atgggaatgt tagcaaaaat caaatcagga aaaaagaaaa tctctgttta
    38221 tggtgattca atcaaagatt caaccgaaga tgtaaaagac cggctaatgc attttgttga
    38281 aaaaatgatg taagagaaac tagcttttga acactattca ttcgaaataa ggggttgtga
    38341 caaaattatt gtcacaaccc ctttaatcta tctttaatta tgttcatgaa gcaataaaag
    38401 atagcaaaac agtaaatcgt tggctttttt tagattgaaa ccagactgtt cttgaaattt
    38461 ttcgattcga tattttagag tattacgatg aagaaacagt gtttttgcag cagagctgac
    38521 attgcctaat tcattccata attcttgaat gatcacttcc atttcatttt gtttgatcag
    38581 ttctatgtaa cttttgatca acttactatc ttttacagta tcttttgtat agtaatgtaa
    38641 agccacatct tgcatagaaa aagtacggct gtgagtataa agattgtttt gctctgttaa
    38701 aaaaattttt ctttcttcag caaacaaagg gacgaggtcg gtgccaggtg aatggaaaga
    38761 accaacgaat acagacgttg tcgtatcaaa atcagcatct aaagatagaa aaataccgtt
    38821 gatttcagta gcatcaagat aatcacggtt tatttgttcg actaagagcg catctgtgtc
    38881 tgtataaaaa aagaaatcag cagaatagcg aaataattcc aaaatagtat cttgccattc
    38941 tttttttaag aattctcctt ttgattcgac gtgaagctgg atcaaacgaa agcttttttc
    39001 ttgaacacaa gaagcatctt taaaaagata gtgataccat gggtgttttt tttgatcgtt
    39061 gattacaggg aataacgctt tcaaaaggct ggtttcttgt tgagataatg aatcttgttt
    39121 gatccagata aaagcatcat caattgcaaa agaaaggata tccgcttctg atgctggaga
    39181 gttttttttc tgtgcttgtg ggtaaagagc aagtaattta tccattttca tcttgttcac
    39241 ctcttgtatt tattttacca tagaaaaaaa agttagttac agccttttta tcatatgcta
    39301 aagtaaaaca agaaaaaaca aaggcaggga agggtaattg gtaaatacaa atcttgatgc
    39361 agtagaaaaa gaaaaatgga tgcgccttgc attggcagaa gcaaagaaag cagaaatgct
    39421 tcatgaagtt ccgattggag cagtggttgt attggatgga aaagtgattg gaagaggata
    39481 taaccttaga gaaaccacgc aagatgctac tacacatgca gaaatgcttg caatcaagga
    39541 agcctgtgaa aaagtgggaa gctggcgctt ggaagatgct gcactttttg ttaccttaga
    39601 gccttgtcct atgtgcagcg gcgcgatgat tttgtctcgt gtagcagaag tttatttcgg
    39661 cgcgtatgat ccaaaaggcg gaacagccgg taccttgatg aatcttttgg aggacgagcg
    39721 gtttaaccac caagcatatg tagaaggtgg cattttagaa gacgaatgtg ggatgatttt
    39781 gactgacttt ttccgaaagc tccgccagcg aaaaaagaaa ttaaaaaaag actggtcaac
    39841 gaatgaaaaa taaggtatac tattctttgc cgaaaggcta ggacaatggt gggcttgtga
    39901 agcgtgtcag gtctggaagg aagcagcact aagcaggttc cgccatgtgt ctgattataa
    39961 tgaaacgaca acagactttc gggtctgttt tttttatttt tatagtactg taaaaatgaa
    40021 tgttcctgat tttttagcca gactgattta tcgaatattg tacttataca atttgcttca
    40081 aggcaggaaa aactctgaaa tagagcttgt ttcaatggaa gaaaaagctg cctatggtat
    40141 gataaagata aagagaggcg agagggtaca tggaaaaaat agtgatttta catacgaatg
    40201 atctgcattc tcatttagaa aattggccaa ggatcagaag gtttttggat caacgtaaac
    40261 gagaaaacga aaagaaagaa aaaacgacaa cgattgcagt agatctaggt gattttgtgg
    40321 atcgttggca tccattgtca gaagcaacga atggacagga aaatatcttg ctgatgaatg
    40381 aagttggtta tgatgcagcg acgataggaa acaatgaagg cgtaggaaat tccaaagaag
    40441 agttgaatca tttatacgat catgctgagt ttgatatcat cctggataac ttattcgata
    40501 aaaatacgct ccagccaccg gcatggacga aaccatataa aatcattaca acgaaacacc
    40561 agacgaaaat cggactaatt gcatttactg ctccatttcc gcttacttat aatccgaatg
    40621 gctgggacat acggaatcct tatgatattt tgccagaatt agtcgaagat ttacgttcac
    40681 aagtggatgt tttagttttg atgagtcatc tcgggattca ggatgatttc caaattgcaa
    40741 aagaaatacc agcaattgat gttattttag gatcgcacac ccatcattta ttcagagaag
    40801 gtcacgtaat caatgatgtc caaatagcag ccgctggaaa gtacgggcag tatgtcggtg
    40861 aagtccattt aaccatcgat gaagagaaaa aaatccttaa gcattcagcc aaagccatcc
    40921 ctaccgaaac aatgacagct tttacagaag atgaacaaga aataaatagt tatctagaac
    40981 gtgggcatga attattaaaa gaaaaagaag tagcagattt gccattttct ctgagtctag
    41041 atatcttcaa tgagcattcg tttatccaag tagcctttga ggcggtgaaa gaacgaggaa
    41101 agacggaagc agcaatatta aacagtggtc tgtttttaac tgaaattcca aaagggcggg
    41161 tcaatcagga tcagcttcac actgcgttgc cgcatccgat gcacttgtta aatgtcacat
    41221 tgggtggaaa tgatctgatc cgcttagttt tagaaataga aaaaaatcgt aactttttac
    41281 gtaactatcc tatgaaggga atgggatttc gcggtaagat ttttgggcag atggtctata
    41341 atggaatcac atatgattct attaaccatc aagtcttttg gaagaacaag ccaatcgatc
    41401 tgaataaaaa ttatactttt actaccgtag atcatttgat gtttgttcca ttttttccaa
    41461 cgattgagat tgctggaaaa aatgaatttt tgttccctga atttatccga agtgtggtag
    41521 gagattactt gaaagctcac tacccaatca aataaaaaca aggtataata aaaaagatag
    41581 gtggtgaacg catgtctgca aaacaaaatg aacagcagaa cgttgagcat aaaacagaaa
    41641 ttgctaagaa aaaatcatca gctgaacagg aaacaacagt caccgaagaa ctgacagctg
    41701 ctttggaaga aatcgttgag gagcctaaag cagaaaaagt caaaggcgag ctagtgacac
    41761 tgttagatga cagtaatttt ttgataggtg aaagacatta tcggcttgtg tccgattatc
    41821 gtgaaggatt taatgcagaa aagcttggag aacgttacag tgatgtacta gcccgatatg
    41881 attatatcgt aggagattgg ggttatgaac aactacgcct aaaaggattt ttccaagcgg
    41941 ataatcgacg ggcgcatcca gatcagcgaa tcgattcctt ggaagactat ctttacgaat
    42001 attgcaattt tggttgtgcg tattttgtca tcgagagaat cggcggtaaa aaagaaaaga
    42061 caactcagcg ccgaaaaaag aaaaaaaacc ataatcgtgc gtatatcgat gagaaaaaag
    42121 gaccagtcgc atctaatcgc aaaaaaccgg ttattaaaaa tcgaaaaaca gagtcagcaa
    42181 aaacctctga aaaagtaaaa aaacaagaac aacctaaaca agtagaaaaa acaaaaacag
    42241 catttacgat cagaaaaagg gaagagtagt ggatgaatgg actataaagg atatttaatt
    42301 gatttagacg gaacgattta tcgtgggaca caacccatac cggccggtaa acgatttgtg
    42361 gaagaattgc aaaaaagaaa attacctttc ttgtttgtta ccaataacac gactaagtca
    42421 ccagaaacag tagctaatcg tctagcagat gagtttgaca ttcatgtagc tccagagaca
    42481 gtctacacag ctactttggc aacaatcgat tttatgaaag cagatggaaa agggaaaaaa
    42541 gtctatgtta tcggagaagc aggattgatt gacttgattt tagctgccgg atttacatgg
    42601 gaagaagagg ctcctgacta tgtagtggta ggcttagata attatctgac ctatgaaaaa
    42661 gtagttaaag caacacttgc tattcaaaaa ggtgctactt tcattggaac gaacccagat
    42721 aagaatatac ctactgaacg cggccttctt cctggagcag ggtcagtcat ctcttttgta
    42781 gagacagcaa cccaaacgcc gccgatctac atcggaaaac cagaagccat cattatggat
    42841 aaagccgttg aagtattagg gctgcaaaaa gaagaagtaa tcatggttgg agataactat
    42901 gaaacagata tccaagctgg gatacgcaat aatatagata ctttgctcgt tttatcaggt
    42961 tttacaaaag aagaagatgt acctgggttg cctatcccag ctacctatgt gaaacaatca
    43021 ctggatgaat ggagcttcta atatgaaaaa ttcgagatgg cagtggatgg aatatgctgg
    43081 attgttcagc ctatttctga cactgatcag tctggcagtt ggtgtgacga tcaactttcg
    43141 accactatac gtatttgata tcggacattt gcaaattttg gattatactt ctttagatca
    43201 agagacgtta ctgaaaaatt ttgatcacct aatgaattat ctcaataatc ccttcaaaac
    43261 catcctcagc ttgccagatt tcccagtttc tgctagcgga gcccatcact tttatgaagt
    43321 gaaaattttg tttttagtgg attatgctgt gtttttcatc actttgatcc cgagtatttt
    43381 gtttatcaaa tatttgcaaa agaatgatcg gctttggcgg ttgattcgtc cgtttcaaat
    43441 cggcatgtta ctgccagttg ttttcggttt tttcatgatg ataggatttg atcgcttttt
    43501 tatccttttt catgaaacgt ttttcaacaa cgatgactgg ttattcgatc ccgttactga
    43561 tccaatcatc aatgtacttc cagaagaatt tttcatgcat agctttatct tattttttgt
    43621 attattagaa ttattttttg ctgtttttct tttcttagga aaaaatagct tgaaacaaac
    43681 aaaaaagaaa gaactcgtct aaatagacgt tttctttctt tttttgttga attattgagt
    43741 aaggcgactt cttttttctg gttctttgtg tgctaatcga ctggcagcag cagcggcgat
    43801 cgctccgacg atatcatcta agaaggtatg gacatgaacg ccatcgtggc tgtttagttc
    43861 ttttaaaata ccaggtttca ccttgtcgat ataaccataa ttcgtaaagc cgatcgtacc
    43921 gtaaacattc acgattgaca aggctaggat ctcatcgata ccgtaaaggc cttcgtcatc
    43981 ttcgataata tgttgcaacg gactcatcaa ttttttcttt tctgctaata tatctaattg
    44041 gattccagtg ataatggcgt tatgtacttc acgctttgtt agcacgctgt tgacactttc
    44101 agtacaatcc gccaaagtca aatcagaaat atagtctttt tgtaaaaaca tgacaagttg
    44161 tgcgatatct tctacagtta cgccacgttc cttcaatagt tctcttgcct tttcttctaa
    44221 agtctcagtt ttgacgacca tattattgct ccccctttat tttttctttc tctatttcat
    44281 gttaacggaa catgaaaaaa atgcaagaaa ctgctttcgg gtttaacttg aaatgcaaag
    44341 agatataaaa aatcaactag gacgaagatt tttatcgtat aagcttgtgc tgtgaccagg
    44401 ctgagttcgt tcctttggat taatatagtg taaagcgttg ttcactgcag taggtgcttc
    44461 accgagccca gtagcaatca gtttcacttt tccttcatag gtacagatgt caccagctgc
    44521 ataaacgcct ggaatagaag tagacatatc tgattttaca gtaatggcat ttctggtact
    44581 atcaagtccc caagaagaaa gatgttctaa attagaagag aatccataat tgacgatcag
    44641 agaatcaatc atcaaatcaa tcgtctcgtc acttttcact ttttttaacc ggatatctgt
    44701 cagttcccca ttattgcctg ataatccatc gatgagatag ggagtaagca agttgacaga
    44761 tgaagatttc aagcgtgata cactatgttc gtgcgcacga aattccgggc gacgatgaat
    44821 caagtaaact tcgcttgcaa taggctctaa catcaacgtc caatcgatag cagaatcacc
    44881 acctccagca atagcgactt tttttccagc atacttcatc aaatcattca cgaaataatc
    44941 caatccgtgg ttttcaaatg attcagcatt atctaaattc aattttcttg gctggaatga
    45001 accatttcct aaagctaaaa tgactgcttt ggaataatgg atgcctttat ttgtagtaat
    45061 ctcgataact tcgtcttcac gagtaagcga gagaacttct tcttttaaat ggaatgtatg
    45121 gttaaaagta gttaattgtt tttctaattg atcgattaat tcacttgctt ttattgctgg
    45181 atacccagga atatcgtaaa tatatttttc gggatacaaa gttgctaatt gaccaccaag
    45241 ttgaggtaga ctgtcaatga tttttgtttt agcttggcgc attcctgcgt aaaaagcggc
    45301 aaacatccct acaggacctg caccgatgat tgtaatgtca tagatttcca taaaaattcc
    45361 tcccttttca cactatatca gaatttaatt gagaatgctt ctcaaaaatg tttgaattcg
    45421 aaatcgaaac ggtaagttat agaatgtatc tagtgatgaa aaataaaaga aaacaaatct
    45481 agtattttta cgagaggtta ggtattatgt tattattatt atgaagtatg caaat
//
LOCUS       DBNICI010000005        44822 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.005, whole genome shotgun sequence.
ACCESSION   DBNICI010000005 DBNICI010000000
VERSION     DBNICI010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 44822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.005"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(291..1787)
                     /gene="lysS"
                     /locus_tag="AC1ZCC_000237"
     CDS             complement(291..1787)
                     /gene="lysS"
                     /locus_tag="AC1ZCC_000237"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIQ1628525.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1898..2902)
                     /gene="dusB"
                     /locus_tag="AC1ZCC_000238"
     CDS             complement(1898..2902)
                     /gene="dusB"
                     /locus_tag="AC1ZCC_000238"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HIQ1628526.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2903..3790)
                     /gene="hslO"
                     /locus_tag="AC1ZCC_000239"
     CDS             complement(2903..3790)
                     /gene="hslO"
                     /locus_tag="AC1ZCC_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HIQ1628527.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4007..6118)
                     /gene="ftsH"
                     /locus_tag="AC1ZCC_000240"
     CDS             complement(4007..6118)
                     /gene="ftsH"
                     /locus_tag="AC1ZCC_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIQ1628528.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6208..6753)
                     /gene="hpt"
                     /locus_tag="AC1ZCC_000241"
     CDS             complement(6208..6753)
                     /gene="hpt"
                     /locus_tag="AC1ZCC_000241"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIQ1628529.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6753..8198)
                     /gene="tilS"
                     /locus_tag="AC1ZCC_000242"
     CDS             complement(6753..8198)
                     /gene="tilS"
                     /locus_tag="AC1ZCC_000242"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIQ1628530.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8318..8788)
                     /locus_tag="AC1ZCC_000243"
     CDS             complement(8318..8788)
                     /locus_tag="AC1ZCC_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HIQ1628531.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8834..9259)
                     /locus_tag="AC1ZCC_000244"
     CDS             complement(8834..9259)
                     /locus_tag="AC1ZCC_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsB family cell division protein"
                     /protein_id="HIQ1628532.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9326..9595)
                     /locus_tag="AC1ZCC_000245"
     CDS             complement(9326..9595)
                     /locus_tag="AC1ZCC_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HIQ1628533.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9606..11201)
                     /locus_tag="AC1ZCC_000246"
     CDS             complement(9606..11201)
                     /locus_tag="AC1ZCC_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative polysaccharide biosynthesis protein"
                     /protein_id="HIQ1628534.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11216..14737)
                     /gene="mfd"
                     /locus_tag="AC1ZCC_000247"
     CDS             complement(11216..14737)
                     /gene="mfd"
                     /locus_tag="AC1ZCC_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIQ1628535.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14754..15314)
                     /gene="pth"
                     /locus_tag="AC1ZCC_000248"
     CDS             complement(14754..15314)
                     /gene="pth"
                     /locus_tag="AC1ZCC_000248"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIQ1628536.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15667..16641
                     /locus_tag="AC1ZCC_000249"
     CDS             15667..16641
                     /locus_tag="AC1ZCC_000249"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HIQ1628537.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16811..18211
                     /locus_tag="AC1ZCC_000250"
     CDS             16811..18211
                     /locus_tag="AC1ZCC_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIQ1628538.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18289..19011)
                     /locus_tag="AC1ZCC_000251"
     CDS             complement(18289..19011)
                     /locus_tag="AC1ZCC_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIQ1628539.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19090..19596)
                     /locus_tag="AC1ZCC_000252"
     CDS             complement(19090..19596)
                     /locus_tag="AC1ZCC_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIQ1628540.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19719..21518
                     /locus_tag="AC1ZCC_000253"
     CDS             19719..21518
                     /locus_tag="AC1ZCC_000253"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HIQ1628541.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21586..23160)
                     /locus_tag="AC1ZCC_000254"
     CDS             complement(21586..23160)
                     /locus_tag="AC1ZCC_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcsB-like coiled-coil domain-containing protein"
                     /protein_id="HIQ1628542.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23415..23924)
                     /gene="mreD"
                     /locus_tag="AC1ZCC_000255"
     CDS             complement(23415..23924)
                     /gene="mreD"
                     /locus_tag="AC1ZCC_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIQ1628543.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23928..24782)
                     /gene="mreC"
                     /locus_tag="AC1ZCC_000256"
     CDS             complement(23928..24782)
                     /gene="mreC"
                     /locus_tag="AC1ZCC_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIQ1628544.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24937..25575)
                     /locus_tag="AC1ZCC_000257"
     CDS             complement(24937..25575)
                     /locus_tag="AC1ZCC_000257"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HIQ1628545.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25576..26226)
                     /gene="rpe"
                     /locus_tag="AC1ZCC_000258"
     CDS             complement(25576..26226)
                     /gene="rpe"
                     /locus_tag="AC1ZCC_000258"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIQ1628546.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(26239..27138)
                     /gene="rsgA"
                     /locus_tag="AC1ZCC_000259"
     CDS             complement(26239..27138)
                     /gene="rsgA"
                     /locus_tag="AC1ZCC_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIQ1628547.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27303..29372)
                     /gene="pknB"
                     /locus_tag="AC1ZCC_000260"
     CDS             complement(27303..29372)
                     /gene="pknB"
                     /locus_tag="AC1ZCC_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HIQ1628548.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29369..30109)
                     /locus_tag="AC1ZCC_000261"
     CDS             complement(29369..30109)
                     /locus_tag="AC1ZCC_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HIQ1628549.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(30122..31480)
                     /gene="rsmB"
                     /locus_tag="AC1ZCC_000262"
     CDS             complement(30122..31480)
                     /gene="rsmB"
                     /locus_tag="AC1ZCC_000262"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HIQ1628550.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31480..32418)
                     /gene="fmt"
                     /locus_tag="AC1ZCC_000263"
     CDS             complement(31480..32418)
                     /gene="fmt"
                     /locus_tag="AC1ZCC_000263"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIQ1628551.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32411..32902)
                     /gene="def"
                     /locus_tag="AC1ZCC_000264"
     CDS             complement(32411..32902)
                     /gene="def"
                     /locus_tag="AC1ZCC_000264"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIQ1628552.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32917..35325)
                     /gene="priA"
                     /locus_tag="AC1ZCC_000265"
     CDS             complement(32917..35325)
                     /gene="priA"
                     /locus_tag="AC1ZCC_000265"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HIQ1628553.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35578..35883)
                     /gene="rpoZ"
                     /locus_tag="AC1ZCC_000266"
     CDS             complement(35578..35883)
                     /gene="rpoZ"
                     /locus_tag="AC1ZCC_000266"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIQ1628554.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35888..36502)
                     /gene="gmk"
                     /locus_tag="AC1ZCC_000267"
     CDS             complement(35888..36502)
                     /gene="gmk"
                     /locus_tag="AC1ZCC_000267"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIQ1628555.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36989..37273)
                     /locus_tag="AC1ZCC_000268"
     CDS             complement(36989..37273)
                     /locus_tag="AC1ZCC_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628556.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37291..38181)
                     /locus_tag="AC1ZCC_000269"
     CDS             complement(37291..38181)
                     /locus_tag="AC1ZCC_000269"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HIQ1628557.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(38201..38419)
                     /locus_tag="AC1ZCC_000270"
     CDS             complement(38201..38419)
                     /locus_tag="AC1ZCC_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HIQ1628558.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38389..40089)
                     /locus_tag="AC1ZCC_000271"
     CDS             complement(38389..40089)
                     /locus_tag="AC1ZCC_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIQ1628559.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40356..41129
                     /locus_tag="AC1ZCC_000272"
     CDS             40356..41129
                     /locus_tag="AC1ZCC_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628560.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(41210..41779)
                     /gene="thiT"
                     /locus_tag="AC1ZCC_000273"
     CDS             complement(41210..41779)
                     /gene="thiT"
                     /locus_tag="AC1ZCC_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HIQ1628561.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41845..41947)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41953..42924)
                     /locus_tag="AC1ZCC_000274"
     CDS             complement(41953..42924)
                     /locus_tag="AC1ZCC_000274"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIQ1628562.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(43018..44391)
                     /gene="glmU"
                     /locus_tag="AC1ZCC_000275"
     CDS             complement(43018..44391)
                     /gene="glmU"
                     /locus_tag="AC1ZCC_000275"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HIQ1628563.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
BASE COUNT        12190 a         9706 c         8109 g        14817 t
ORIGIN      
        1 ttagtaacat gtcaataact tttttgttat gtttttgaat aaatattttc ttctgtcttt
       61 tggacaagta ataatatacc aactaaattt agcttggtca acgaattttt tgtatttttt
      121 caagtttttt tcaataaacg attattcata acttttcttt ctttatcgtt cgttttcgtc
      181 ttctttaatg tctagaatag tcggcattag cttttgaact ttcgttatta tttattcctt
      241 tctacacagc aaaagctgcc taaggaattc cttagacagc ttttattcga ttatctcatt
      301 gttgggaata gcaaaacatc ccgaattgat tgtgcatcag ttagcaacat tactaatctg
      361 tcgatcccga tgcctagacc accagttggc ggcataccgt attctaaggc ttccaagaag
      421 tcttcgtcta caccgtgagc ttcgtcattt cctagttcac gttctttttc ttgcgcttcg
      481 aatctttctc tttgatcaat tggatcgttc agttctgtaa aggcattagc aaattcgcgc
      541 cccactatga atagttcgaa tcgatctgtg aatcgaggat cttctggatt tttcttcgct
      601 aaaggcgaaa cttcgactgg atgtccgtat acaaacgtcg gttgtgtcaa tgtttcttca
      661 acaaatgttt caaagaattc attgataaca tgtccgacac ccatagcttc ggttacttct
      721 acattgtgtt tttcggctaa agctaatgct tcttccaatg tcatttcttg ccagaagtct
      781 acaccagttt gttctttgat tgcatctacc atatggatgc gtttaaagtc actttctaag
      841 tcgacagctt gtccatcata tgtgattttt gctgttccta gtactttttc agctgcgtta
      901 cggataatac cttctgtcag attcattaca tcattgaaat cagtatatgc tgtgtatgct
      961 tctaacatcg taaattctgg attatgtgta gtatcaattc cttcattacg gaaaacgcgg
     1021 ccgatttcat aaactttttc cattccgcca acaattagtc gttttaaatg aagttctagc
     1081 gcaatacgca aatataaatc aatgtccagt gcattatgat gcgtgataaa tgggcgtgcg
     1141 gcagctccac ctgcctcatt gtgcaatact ggtgtttcta cttcaatata gccattgcca
     1201 tctaaatagc gacggatttc gctgataatt tggcttcgtt tcataaatcg gtcaaagctt
     1261 tcgcgattac tgattaaatc caagtagcgt tgtctgtagc gttgttcaat attcgttaag
     1321 ccatggtatt tatctggcag tggacgaagt gcttttgaca aaatcgtgat ttctgaagct
     1381 ttgatagaaa cttcgcctgt gtctgttttc atgatttgtc cagttacacc aaagaaatct
     1441 ccaaggtctg catgtttgaa tacttcatag gcctcatcac ctacttggtc ttttcttaca
     1501 taaatttgaa tttggccttc acgatcttgt agatgcgcaa atccagcttt cccttttcct
     1561 cggcgagtca tcatacgacc agctacacta gcggtcaatc ccatttctgc tagttcttct
     1621 tttgttcttg gatcaaatag ttcatgcaat tcttctgagt tgtgcgtgcg ttcaaatcgt
     1681 tttccaaatg gatcgattcc atcttcacgc aattgttcca ttttttgacg acgtacaagc
     1741 atttgatcat ttaaatcttc tacttgatgt tgttgttcct gtgtcacaaa agttcctcca
     1801 ttctcatgtt aatctctttc ctataatgcc aatgaaacac aaaaaaagca agccgaactt
     1861 ggaatttcgg cttgaaaatt cacgaaaaac ttagaaatca caacgtttgt ttcaatagta
     1921 ctttttccgc tttttctaca aattgatcca gcaggtcatt gatttcttgc tgtttttctg
     1981 tctgattgat tgctactttc actttcgctg ctcgaggaat cccttttaaa taataagccg
     2041 catgtgttct gaattctcta caagctactt tttctccttt gagatctgct aagcgttgta
     2101 aatgaagttt agctgtagca attttttcgg ctggtgttgg ttcaggaatc agttcccctg
     2161 tttcgagata atgctgtgtg cggtgaatca tccatggatt gcccagcgct gcacgaccaa
     2221 tcatcacacc gtctgcaccg acatagtcaa gcatccattt tgcatcttct ggcgttttga
     2281 catccccgtt ccccataaat ggaatcgtca aatgtttttt cacttctttc aatacttccc
     2341 agtccgcttt tccttcatac atctgaaccc gtgtacgtcc atgcatagcg atggcacttg
     2401 ctccagctga ttgtgctgct aatgcatttt ctacagcaaa gacgtgctct tcatcccaac
     2461 caatacgcat cttgattgtt actgggatat ttactgctga agaaacagct tggaccattt
     2521 cgtatacttt ctcaggatct aaaagccatt ttgctcctgc ttctgctttg attactttat
     2581 ttactggaca gcccatattg atatctatta tatcggcagc tgtgttttct tcgacaaatt
     2641 gagcagcttc aactagacta tctttatttc cgccaaatat ctgtaaactt aatggatgtt
     2701 ctgtttcttc aatgtggagc atctctaaag tttttttgtt tctgaaatga ataccttggt
     2761 cactgatcat ttcacatacg actaagcccg caccaaattc tttgacagtg actcgaaatg
     2821 ctgaatttgt tattccagcc atcggtgcaa caacgacacg attagggatt tccacattgc
     2881 caattttcca tgtgtgttcc acttaaatcc ctcctaaggc ttctttcttc aatccatcta
     2941 attcttcttc gctatacttg tatttgttat tacagaaaga acaaaccgct tctgcaccgt
     3001 gatcttcgtc tatcattgct tggatttctt ctgcacctaa cgcgataatt gcttctgcaa
     3061 atttttcttt tgaacagtca catttgaatt gaacaggcat tttttccaaa atgttcaatt
     3121 cttctgtacc aagcaatcct ttcaatattt cttctggtgt ctgtccttca tctaagaggg
     3181 tagagattct tggtgtcgtt tttaattgtt cttcgatctt gctgattgtt tcttcatcgg
     3241 cacctggcat gatttggatc atgaatcctc ccgccgtctt gatgctgtcg tcagtattga
     3301 ccaatacact cagtccaaca gctgaaggga tttgttctga gatagcaaga tagtaagtaa
     3361 aatcttcagc gatttctcca gaaacaatcg gcgtttgccc agaaaaaggt tcttttaatc
     3421 ctaagtcttt tgtgacagta aacattcctt gtgttccaac tgctccccgc acatcgattt
     3481 taccttcact atttagagga agactgagat gcggattttt gatgtaacct ttcacatctc
     3541 cctttccatt tgcatctact acaattcctg ttgctggacc atcgccttgg attcgaacag
     3601 ttagtttgtc ttctcctttg agcgtcgcac caagtagcag actaccgacc aatgtccgtc
     3661 ccaaagcagc tgaggaggtg tgccaagtat catgacgtct ttgtgcctcc gctacggtat
     3721 ttgttgcttg aacggcgtat gctcggacga atccgtcgtg agcaagtgcc ttaaccaaat
     3781 aatctttcat ttgttatgta ctcctttttc cgtttttaaa ctcatagtga atcatactag
     3841 caactactgc ttttttcaac tttggaatgc ttcttttaaa aataattcag aatcatctag
     3901 aaaacaggac atcaccctat ttacaactta aataaagaaa tctcccgaaa accggttttt
     3961 gttccggttt tcgggagtca tatcagatca attgacctat atttttttat ttgtattgat
     4021 cgtctgaatg attatcttct gattgttgag aatctgaatc atgttctgct ttattttcct
     4081 cattcagttc atgttttgct tcttcaaaat cacgtttttc ttccgcttgt ttttcagcgt
     4141 ctttttcttc caaagcacgt tttgcttctt caaaagtttt cgcttctttt tcgcttggat
     4201 aatcatttga ctcagtacct tcaggcatca caccgtgttc aaacaatgac ttgattgctt
     4261 tggcatccaa tgtttcgaat tccaaaagtt tttcagcaat cagtttatgc tgtgcacgat
     4321 gagcttcgat gatttctcga gctttatcat gagcttccat caagattttt cgtacttctt
     4381 gatcaatctc gaaagcgact tgttccgagt aagcttttgt ttgaccatag tcgcggccaa
     4441 caaacacttg atggtttcct tcatattgaa caggacctaa acgatcactc attccatatt
     4501 ctgttaccat gcttcgtgct agagctgtcg cttgctcaaa gtcattggaa gcaccagttg
     4561 attggacatt gaagatgatt tcttcagctg tacgtccacc aagtaagcct acgatttgtt
     4621 caaacatatc ttctttcgtc atcaagaact gatcttcttt tggtaaagcg atcatatatc
     4681 cgccggcgcg tccgcgaggg atgatcgtta ctttgtggac gacccgagcg cgactcaata
     4741 ctaatccgac aatcgtatga ccagcttcgt gataagcaac catttcgcgt tcttttttgc
     4801 tgattacgcg atctttttta gctggacctg caatcacgcg atcttccgct tcatcgatat
     4861 cagaagcatc gattttcttt ttatttcttc tagcagctac taacgctgct tcattcaaga
     4921 cattttctaa atctgctccg gcaaaacctg gtgtctgttg agccacaact ttcaagtcaa
     4981 cgtcatctgc catcggttta ttccgtgcat ggactcgcaa gattgcttca cgacctttta
     5041 catctggacg tccaactaaa atctggcggt caaaacggcc tggacgtagt aacgctgggt
     5101 ctagtacatc agaacggttg gttgcagcaa tgacgattac tccttcattt ccgtcgaagc
     5161 catccatttc tacaagcaat tggttcaatg tttgttcgcg ttcatcatgt ccgccgccca
     5221 taccggcacc acgttgacga cctaccgcat caatttcatc aatgaaaatg atagctggtg
     5281 cgtttttctt cgctgtttca aacaaatcac gaacacgact tgctccaaca ccgacaaaca
     5341 tttctacgaa atcagaaccc gagatcgagt aaaacggtac tccagcttct ccggcaacag
     5401 cttttgctaa taaggtttta cctgttcctg gaggtccttc taaaagtaca cctgccggga
     5461 tacgggcacc taattcaaca aatcggcgag gatctttcag aaattcaaca acttcaacta
     5521 gttcttgttt ttcttcttcc gcacccgcta catcagaaaa acgtacgcga ttagcttttt
     5581 tgtctgcttc tttggctttt gatttaccaa agttcatgac acggccgcca ccgccaccgc
     5641 cgccgccttg ttgtcccatc atcatgtaaa agaaaaagat gatgattacg attggcaaga
     5701 agctaagcaa tagggaaacc catataccgc tgcttgattc ttcttttact gttgtagcaa
     5761 ctttgttttc ttttgccagt tctgttactt ggctcaatgt tgtatcattt ggcaaaatga
     5821 ttgttgtgaa atgggtagta gtcgtatctg ttgaacctaa gatagctaac ccaccgctgt
     5881 tagcgacggt ctgctgttct ttgtattcac cagtgatctt atatacacca tttgcaggct
     5941 gcacttcaaa ttctttgatc ttgcctgctt gtagttgttc tgtgaatttt gagtactcga
     6001 tatctgacga tcgctggccg ttattaccaa aaatgaaata aacgaccatt accatcgcta
     6061 agattacgag gacataatac aaggcatttt tcatcatgcc gttgttcttt ttgttcatag
     6121 ttgccctccc taagcgtcaa tttaatttaa cagtttttta tcaatcctga ccattctatt
     6181 gtaccataat ttaattgaga tacagcatta atttgattga taaacactag gtttcaatac
     6241 accgatgtac ggaaggttac gatatgcttc tgcataatcc aagccataac cgaccacaaa
     6301 ttcatttggt acgttgaagc caacataatc tgcttcgata tttactactc ttccttcagg
     6361 tttgtcaagt agtgtaacaa ttttgactga ttttgctttg cggtatttga aaagatccac
     6421 taaataagca agtgttcttc cgctgtcaat gatatcttca acgatcaaca gatcgcgacc
     6481 ttctacattc gtatctaagt ctttgacgat ttttacttcc cctgacgaga ctgttgcatt
     6541 cccgtagctt gaaacatcca tgaaatccaa ttccaaatac gtatcgatag aacgaacgat
     6601 atctgccatg aatggtaccg ctccttttaa gacacctact actaggggat tggtgttttg
     6661 gtaaatttct gttaactctt tgcctagttc tttgcaacga acttgtatct cttcttgtga
     6721 gatcaaaatt ctctcaatat ccttttccaa catcaggttc tccttccaat tgcttctttt
     6781 tgatatttgt aaagaagtat gtagtgtatt ttatcagttt cttcacgaat actcaaatat
     6841 gaaaaggcaa aaggtatcaa gctatatata tttcttttgg catctgtaat gatccaagca
     6901 tgctgccttt gtgaattagg tactttctga tcgataaaat atctagatac tttttttgtg
     6961 agattctctg ttaatttaat gcggtctcct gcttttcttt tttcgacata cagttgcaca
     7021 tcagcaggaa tccaaatgtc atgagaaaat agagaccatg aatctgttgc tttgatgtcc
     7081 cctgcatttt caggtttgac cagaccaatc cattctgttt cattcaaata tattccttgt
     7141 tctggaatca gcaacaagcg ctggtcttct gtctggctag ttgcttgttt ttctttttta
     7201 gagagtacaa caaaatgata ctctcttttc agtatccaat tattgccaat actgagttgc
     7261 cattggcttt ttttctgttg ccactggtct aaaatcgact ccatttgttg ttctttcaaa
     7321 ataattcctg tttttgagaa tgctttgtcg aaaaaggagt aaaaaaaata gtatcgttca
     7381 gcatctgtca tttcttcaat catttctgca gaaaatcgaa attgttcctc ccactgttcg
     7441 acttcctttt cgatcaactg tcccatatat ttttgaatga cttggtctgc ccattggatc
     7501 tgttgcgaaa attgctggaa atgtctcaac gcctgtgtat tttcttgttt caattgagga
     7561 actaccaaat gacgaagccg atttctctgg atatctaaca attggtttgt ctgatcctca
     7621 aagtaaacaa gctgcgattc cgctgcataa gtatataggt cttctttgct aaaactcagc
     7681 aacggacgaa tcagtctgcc cgtagcaaat ggttgtactt ctttgattcc agaaaaagta
     7741 tttaactgtc ccccacgaac gattttcatt aacaccgtct cgatttgatc atctccgtga
     7801 tgagcagtca tcaacgtgtc atattgtcct tcttccatca cgcgagtgaa ttcttgataa
     7861 cgaaagttac gagcttctgt ttctatggcg gatttggctg gatcttccca aatacttaca
     7921 tataaaggga gattcttctc ttcacaatat gagcgaagat aggcagcttc ttctgctgat
     7981 tcttctctta attggtgatt gatgtggacg atacctaaag aaaaaccgat ttggcgttgc
     8041 acttgtaaaa gtaaatggag caaaaccatc gaatcaacac cacctgatac cgctgctaaa
     8101 attctcgatc cttgttgcca gaatccttgt tcattcccgt gccgaataaa tgactgctgt
     8161 aatagcggtg cttttttccc gcctttttca acgttcatcc tttccacacc ctttcttcct
     8221 aaaaaaaaga gagcgggaaa acgtctaaaa cgacaattaa cctgccctct gttgatacta
     8281 tgacccaaat agtcgcacta tcattatttg cggcttttta gctgcgacgt ccgccacggc
     8341 caccgcgctt tccttctgtg tttcgcttca atgaagataa gcggtcatcg ctatctttca
     8401 aaaatgaact catcaatgaa tcgaagtctt ctttttgagc tgaaggttga ctgcgagaaa
     8461 aagatttttt ctgtgctgga cgatttcggt tgtcacgggg atctttttgt tgatattttt
     8521 taggacggct ttcttctcca gattcagcag gttgttcctg cgctttccgg atagatagtc
     8581 cgatttttcc atcatcattt actgaaataa ctttaactgt tacttcatct cctacactta
     8641 aaacatcatg gatgtctttt acatagccat ttgacacttc gctaatatga actagacccg
     8701 tttttccagc acctagatca ataaacgcac caaaatttgt gattcctgat acttttcctt
     8761 gcagttttgc tcctacctcg attgacataa aaaaaatgtt cctcctaatt atttcccttt
     8821 tttatattcc tgcttatttt gcagttgtag aactagttga ttgatctgtt tcatttgtcg
     8881 agctgtctgt atttgattct aatataggat aaactttttc accgtctttt gagtaaaaga
     8941 atttgcttct ggcaagttta gctacatact catcatcttt cagcaatgaa acatcttggt
     9001 gcagctgatc aacgtctgcg ttgacttctt tcatttcagt atttgcttcg actctcaatt
     9061 cgttcaattg atgcatcctt tgcatgtcat ggaaaatctg cacacctatt atcgcgaaaa
     9121 tgaccaaagc accaacaaaa attgcagcca gtctgcgtct tcgaaaaatc agttgacgtt
     9181 gttgttgctg aaattcagca tactttttct ttgcatattc cgtatctagc gcagcgattt
     9241 tattgctttt tttcgtcact tggattcgct ccaatctttt tgattgtctt tttcattata
     9301 ccaatatcgt gaaagcttgt ctaatctatt tctctagtgt ttctgtgcga ttttcagaaa
     9361 taatttcgta cattttttgt gcatcttctt ttttggttga ttcatgtaat tccgtaattt
     9421 tcacttccaa cgttttgttg ccaaactgga ctttcaaaat atcgcctact ttgacatcac
     9481 tggaagattt cgccaattta ccgttgattt gaattcttcc tttatcagct acttcttttg
     9541 ctactgatcg tcttttgatg attcgggaaa tttttaaaaa tttatctaat cgcatatctt
     9601 attcctcact ttttaatttt tttatttaat cgcaggattt tcttaccaaa aggcaacatt
     9661 agccattctc gtactgtaaa cagacgaaga cgaatcactg cataaatgaa cacagaagcc
     9721 ccgatcccaa ctcctatcac gctgacaaaa aaagcagtgg aacgatgagt tacaggacca
     9781 aatactatat tcaatacacc ataatacagc agcagtacta ctgtcataca aagaaggctg
     9841 aacatcaact tccgtccaaa atgccgttct ttccaaaaag catttagctc ttttgtttca
     9901 attcgaacaa aataccaaag cgtcacacct aatcctaaaa gggtagacag gcttgcacct
     9961 gctgttccta ataaaccggt cagaaaagaa gtagtcaatc cttttaccaa tagtccccat
    10021 cctgcgcctt ttaaagaagg gcgaaacgag ttcttgcttt gagcaatact ttgataagct
    10081 tggatgactg cagtgaaagc aatggaaaaa acaaataata caagcgctgc atttcccgca
    10141 taatctttaa acaacgcata attgatataa gggagcagca aggctaatcc caaagacgca
    10201 gctaatgcta aagcggtcgt caatctaaga tagattttgg ctgtctgtaa aaactgacga
    10261 tagatccggt tggtcaaatg acgagtcaac gcaggcagga atgtcgcact taatgcagtg
    10321 gcaatcacta aacctaattg tactaaaggc tgacctcgat catagacccc ttttgctatc
    10381 ttcgcaccgt attctgacag tccgtaaact tccaaagcat tttttacaaa gaaagagtcg
    10441 atcagctgga aaaagataag caatccacta taaatcgaaa ccagtccgcc ttctaccaga
    10501 aaacgccgga tcaagggctt tgacggctgt ttgaacaatg gaaaccgtcg gaagcttaac
    10561 gctcctccat gaatcttttg ttcatagtag tgaaggatcc catatgcgca taaaccgcca
    10621 aaaacagctc cactcattgc tgccgtccct gtttggtaca ccgtccatcc aagctttcta
    10681 aaagcaaacg cactacagac aatcacaaaa acacgaacga actgctcaac tacttgagaa
    10741 acagcagttg gttccatcaa gaatcttcct tggaaattcc ctcgataaaa agatagacca
    10801 ggcatcagca aaaaggtaaa ggaaactact tggatcagcg gtttcaattg tatgtctccc
    10861 atcatccaag caatccagct gctaaatgca aagacaaaca accacaaaaa gacacctgtc
    10921 caaaatacta agggataaag attttgcaaa gcttgctttc gctcgaaagg attctttctt
    10981 tctgcaacgt actttgatat gaactgcgga agaccggata aagctaaagt cattgctatc
    11041 ccataaattg gataaacttg ctgataaaca taaaaacctt cgtcgccgac taagttttgg
    11101 aggggaattc ggtaaagggc acttaatact tttgctatga aagaagcaat cgtcaataca
    11161 aatgctcctt gcatgacgcg ccgcatttct ttatgatcca aatcattgct ccttcctacg
    11221 ctgacagata tttttgctca cgcaacgctt tgacaaactt ttggatttcc tgaagccata
    11281 ctgcttcttc catttggttg gggatcgtca agcgtatcac catctgttct ttttcaacgc
    11341 ctagactggc ttttagtttt gtcgctgtca gtgcttcgaa taactgttcc acactatagc
    11401 gttttgttcc aactttactt agagtaaaga ctatatgcgc ctcctgtttc cttatcgttt
    11461 ctaggagcgc tcgatcgcca tccattttga tctgcccaat tgtcaagaga tgtgctactt
    11521 cttctggata ttctccaaaa cggtcgagca gatcatcttc tagttcatct agcatttctt
    11581 gcgaatcaag ttcacgaatt cgtttataga tctcgatttt ctgccgttga tctgccacgt
    11641 atgtttcagg tagatacgca tcgacaccga ggtcgatctc aacagtggtc ttctccactt
    11701 gactattctt tccttgttta cgagaaacag cttcgcttag catttgagaa tacatatcga
    11761 aaccgactgc atcaataaat ccatgctgct gagcacctaa gagattacct gcaccgcgaa
    11821 tggaaagatc ccgcatcgca atcttgaatc cagatcccag ttctgtaaaa tctttgattg
    11881 cctgaagacg tttttcactg acttcgttaa ggatcttctg ctgctcatac ataaagtaag
    11941 catatgcgac acggctgctt cttcctaccc gccctcgcaa ttggtataac gtggataatc
    12001 ccatataatc agcgttctca acgaacaacg tatttgcgtt cgggatatct actcctgttt
    12061 caatgatggt tgtagtgaca agaacatcat attgaccttc aataaattca aaaagcgtat
    12121 tttctaattg cgcctctgtc atttgcccat gagcataacc gatccgtgct tccggaacga
    12181 gttcttggat ttcttctact ttttgttcga tcgtttctac ccgattatat aaatagaata
    12241 cttgtccacc tcgtcccatt tctcgatgga ttgcttcgcg aatagcccca ggattttttt
    12301 ccataacata cgtttggatc ggataacgat tcgcaggcgg tgtttcgatt actgaaagat
    12361 cccgcacgcc taacatcgac atatgcaaag ttctcggaat cggtgttgca gtcagagtca
    12421 aaacatccac ttgtgcacgt aactgtttca atcgttcctt atgttttaca ccaaaacgtt
    12481 gttcttcgtc aatgatcagt agacccaaat cgctaaattc aatatctttt gacaaaattc
    12541 ggtgggttcc tacaacaata tcaacttgac ctgtacgcag ttgttcgatg gtttcttttt
    12601 gttgctttct tgttctgaaa cgacttaata gcccgatatt tacggggaaa ccttcaaatc
    12661 tttccagcat cgtttcatag tgctgctgtg ccaagatcgt tgttggaact aaaaaggcta
    12721 cttgtttgct ttcctttatc gctttaaagg ccgctcgaag cgctacttct gttttcccat
    12781 aaccaacatc acctacgagt agtcgatcca ttggtttttc tttttccata tcccgtttga
    12841 tctccgctgc acttctcaac tggtcatcag tttcagagta ggggaaggca ttttcgaatt
    12901 ctttttggta ggcgtcatca ggtccaaaag catatccttt ttctgactct cttgcggcat
    12961 ataatttgat tagatcgtca gcgatatcct cgatttttga agagactttt cgcttggttt
    13021 tcgtccattc acttccgcct agcttattga tgcgaggcgc tttggcttca gaggcgacat
    13081 atttttggat cagattcagt tgagtaacag gaataaacag tttgtcgtcg ttttgataca
    13141 aaatagtcat atagtcttgg tgaacgccat ctacttctag cgtttccatc ccaatgtatt
    13201 tcccgatacc atgattggca tggacaacgt aatctcctgc ttttaactca ttatagcttt
    13261 tcaatcgctc tgcgtttgtt actgtttgac gtctcgtttg ttttttcgtc gttttttgga
    13321 aaatttcttt ttctgtgata gctactattt tttcttgagg aagttcaaaa ccagcctgca
    13381 aagctccttc aactaattgt acctgtcctc tgatcaatgc atttttttct gtttctacta
    13441 ctaatatgtc ttcatctcga agcatttgtt ctgctttgcg tattctgtcc tttgtcggaa
    13501 taaagatcac aacagtctgt ttttgcttgc gccaacggtc catttctgtt ttcagcaatg
    13561 gcatttgtcc gaagaactgt tgcatcgtcc gatattgaaa attatggatt gcttggaaac
    13621 gaagatttcc catccctttt tggaagagtg agaaaaaggt tgtggtgaac ttttccttct
    13681 gcacgatttt ttgtatatct gctgaaaatg tctgctcaga aaacacccgc aattcgctga
    13741 ttttttgagt ctgccattct gccgcttccc tttcgatttc ccgatttgtt tccatgattc
    13801 gctgataatc atcaacgact agtaaactat ttgaagggaa ataatccaag atcgtcacag
    13861 attcttggta aagaagatct gagtagaatt ttgcttcttc tgtcgggaca cctgcttgcc
    13921 agcttgaagt cagctgaccg aagtaatctt gcaaaaactc gcggtctgct tcttctgtgg
    13981 tcgcagcaag ccgtttttct agcatttttt gcatttgctc tattccatgt tctagatcct
    14041 ctggtccgta gacttgttca ctcgttgggg aaacccagat ttcttccgtt ttttcgattg
    14101 agcgttgtgt ttctgcttca aaataccgca tagaatctat ctctacatca aatagttcta
    14161 cacgaacagg ataggttgtc gttaacgggt aaatatcgat aatgcttcct cgaatactga
    14221 attctcccgg ttttgcgacc atctcttctc gtacatagcc catcaaagtc aattggcgag
    14281 gaagattttc cacgtctatc tcatcaccaa tatgccaatt caattgcgcc tgtagccaag
    14341 ttttcttatt cggcagtcgt ttgtgcaaag atgcgactgg caacaaatag atacctgctt
    14401 ttttttcttg aagtgcgtta agcgttgcaa ctctctcgcc tcgcgcttct ggagaagaaa
    14461 aggccatttc agcagaaagc acttcatcca ctggaaataa atggatctga tcttgcggaa
    14521 tcacatgttg cagatcttct gctaactgat tcgcataata caaatttggc atcagaataa
    14581 tcatgttttt atttgttttc ttaaacgctc gagcaaatag aagtgttttc gctgagcccg
    14641 ctaatcctgt gattagttgt ctaggcattt tttcttctaa attgctgacc cattctttta
    14701 cttgcggatc ttttccgact aattgaataa tatccattcc ttttcctcct tcctcaattg
    14761 aatcgattca tcgcatccac gaacgaatgc ccttctatga gatattcgac tgctttaaca
    14821 gattgacaca tgctttcttc tatcaaaggc tgattttctt tgctgaaagg actaagcaca
    14881 tgctggacta ctgttttttt cccttctggc ctgccgattc ccaccttgat tcgatcaaag
    14941 acattcgtat tcaaatggga aataatactt ttgataccgt tatgcccgcc ggcacttccc
    15001 ttttgacgga gccggatttt tcccactgcc agatccaaat catcgtagat tacgactaat
    15061 tcctctggat aaatcccaaa ataagtcatt agtgggccga ctgcacgacc agattcattc
    15121 atgaatgttt gtggtttgac taaaaggatc ttttctccat tatgaaaaaa ttcggcaact
    15181 tctgcttcga acggattttt tttgaaagta gcttgatgct cgcgtgcaat tttatccacg
    15241 accataaagc cgatattgtg ttttgtatat tggtatttgg tacctgggtt tcctaacccg
    15301 acgatcattt tcatttttgt tttccaccta attctaaaaa gcagcacaaa aggctggact
    15361 gctaattagc ggtccagtcc atcgagcttt tttctttttt acacttgtca atgcatagta
    15421 taacacaaaa caaggcgttt cggctaaaag agaatcgtta ataaatgata aagaaattaa
    15481 aaatttcttc aaagtttaat gaaataattg atatataaaa atagtgaaat aacgcacatg
    15541 taagctttta catataatac aaccttgtta tctgttatat tttatagcca aatagcgtga
    15601 catggggctt gagaaatgtt agcataagca tgggaaaaaa caaatcaatg gaaggattga
    15661 tactaaatga ctgcagctgc agaaaaaaaa gatcatcaaa aagttattct tgttggagac
    15721 ggcgccgtag gttctagcta tgcctttgct ctagttactc aaaatatcgc tcaagaagta
    15781 ggtattgtgg atatcaatac tgctaaaact gaaggagacg caattgactt gtctcacgct
    15841 ttggcattta cttcacctaa aaaaatctat tctgctactt acgcagacgc acatgatgct
    15901 gacttagttg ttattacagc tggcgcacca caaaaaccag gtgaaactcg tttagactta
    15961 gtaaacaaaa acctaaaaat caaccgcgat gttgtcacac aaatcgttga atcaggattt
    16021 aacggtatct ttttagttgc cgctaaccca gtagatattt tgacttactc aacttggaaa
    16081 ttctcaggtt tccctaaaga acgcgtaatc ggttcaggta cctctcttga ctcagctcgt
    16141 ttccgtcaag cacttgcgga attagttgac gttgatgcac gtaacgtcca tgcttacatc
    16201 ttaggtgaac acggagattc agaattccca gtttggtcac atgcaaatgt tgccggcttg
    16261 caaatctatg aatgggtaaa aaacaaccca gaaatcgatg aagaagctat ggttaaccta
    16321 ttctttggcg tacgtgacgc tgcttacaca attatcgaga aaaaaggcgc tactttctat
    16381 ggtatcgcag tagcattagc acgtatcact cgtgccatct tagatgatga aaatgctgtt
    16441 cttcctttat cagtattcat gaatggcgaa tatggattga acgacatcta cattggtgca
    16501 cctgctgtta tcaaccgtca aggtatccaa aaagtaatcg aaattccttt aagtgacagc
    16561 gagcaagacc gtatggctgc ttctgctaag caattaaaag aaattctaga tgaagctttt
    16621 gctaaactag acgctgaata atctttgaac atgacggaaa acatctattc ggtgttttcc
    16681 gtttttttat tttatcaccc ttatggtttc taaatttttt cataaagtag agataacctt
    16741 cgtcttttcc tctaaattat gctatcatga gaccgttcca ccctaaactt ttacttaagg
    16801 agttgtcgat atgacaagat cttcgcatcg aaaaaaatcg aaacatgttg ctttgctttc
    16861 agttcttggt gttctcttag tgctgatcag cttttatacc tatcgcagta catactacac
    16921 aaagcatttt ttgccgaata cagaaataaa tggaataaat gtcagcaatc taacagtcga
    16981 aaaagcaaat gaaaaattaa aagaagctta ttctgataaa aaactggcta ttaaagaaga
    17041 tggaaaagtc tggcaggaag taccaaaatc cgagctcggc tacaaagatg atttcacttc
    17101 ggagctttct ccgatcctaa atgaacaaaa cggatggact tggggaatga cttatgtctc
    17161 agcggcagaa aaacaagaaa tagatcctgt cagtcaaaac caccaaaaac tagacactac
    17221 cgttcagaca ttgacaacta agctgactga tttgaacaaa gaccggacac ctactactga
    17281 tgcaacgatc gaaaaagcag gtgactcttt cgttatcaag cctgaagtca atggaaatac
    17341 gatcgacgtc gatgctgcgg tcaaacaatt aaaaagtgcg gtaaactctg gtaaagatac
    17401 aattgaattg actgaattca aagaaaaacc aaaagtaaca tctgaagaca gctcactgaa
    17461 agaacaactc gcatccatga atgctatcgc aaatgtaaaa gctacttaca gtataaacgg
    17521 tgagactttc cagatcccat caagtgatat tatgagttgg ctgacctaca atgatggaaa
    17581 agtcgattta gatacagaac aagtacgtca atatgtaaca gatttaggca caaagtataa
    17641 cacaagtacg aatgacacaa aatttaaaag tacaaaaaga ggagaagtta cggtcccagt
    17701 tggcacttac agttggacga tccaaacaga cagtgagaca gaagcattga aaaaagcgat
    17761 tctggcaggc caagatttta ctcgttcacc cattgtgcaa ggtggcacaa ccgctgatca
    17821 tccattgatc gaagatacgt atatcgaagt tgatctggag aaccagcata tgtggtatta
    17881 caaagacgga aaagtggccc ttgaaacaga tatcgtttcc ggaaagccaa ctacacctac
    17941 acctgctgga gtcttctatg tttggaataa agaagaagat gcaactttaa aaggaacaaa
    18001 tgacgatgga acaccttacg agagtccggt aaactactgg atgccgattg actggacagg
    18061 tgttggtatc cacgattcag actggcaacc tgaatacggt ggagacttat ggaaaacccg
    18121 cggttcacac ggctgcatca acaccccacc aagtgtaatg aaggaattat tcgggatggt
    18181 cgaaaaagga acgcctgtat tggtcttcta acaaaaataa tatataaaaa gaaagcaccc
    18241 gtacgattgg tacaggtgct ttctttttat tatctaacaa tgtgctactt acatttcagc
    18301 agcagtaaag acttctgaaa catcatcatc gtcttctagc ttatctacta gacgttccaa
    18361 ttgctctttt tgttcctcat ttaaatcaat cgtcgtttgt ggaatcatcg tcaattctgc
    18421 ttgcgccaaa gtgaactctt tttccaactc atctcgtaca gcagcaaaat cttctggtgc
    18481 agtataaatc tcgaatactt caggagaagt ttgcagatct tctgcccccg cctctaatac
    18541 atcctcaaac atcgtctctt catcaacagt caacccatcg cgttcaatag cgatatatcc
    18601 tttgcggtca aacaagtaac tgactgaacc cgtttctccc agtgatccac cgtttcgagt
    18661 aaaagctact cggacatttg tcgctgtccg attacggtta tctgtcaatg catggactaa
    18721 gattcctact ccacccggac catatccttc atacgtgatt tcatcgtaat tcgtttcatc
    18781 tacggcactc gtagcctttt taatcgcccg ttccacattg tcgttaggca tatttgcagc
    18841 ttttgctttg tccacaacaa gccgcaaagc tggattcata gatggatcag gaccgcctgc
    18901 tttagctgcc atataaattt cacgagaaag tttctgaaag actttccctc gttttgcatc
    18961 ttgggcattt tttcttcctt ggatattatg ccatttacta tgtcctgaca tgatttttcc
    19021 tcccttgttc ttttgagaat acacttctat tctagtacta tttcacaaaa aaacaaagcc
    19081 acagccaatc tataaacgct tcttgaatgc cgcaaaaagc acacataata tcacagcagt
    19141 cagagcaccg atcgaatcca acatgacatc ttggaacata ggtgttcgtc caccggtcat
    19201 catctgatgg aattcgtcca aacctgcgta ccctgttgct gaaagccaag caaggatcgc
    19261 tgtcagccac caaattttta gcttggggaa aacacctaaa aacaagctgc ctcccaaaat
    19321 aaaataggta ccgaaatgtg cgcccttcct taaaaagaac tctaaaaatt tggcgtagcc
    19381 cagatggttg atactcacca tacttccacc ataattaaaa cgtatacccg ctaatgtgtc
    19441 tttaaatggc tgattaggca gccagctttc taatcgcgaa acttgtgatt gctgtccgta
    19501 agtttgagaa gaactgtaaa ataagattat aacgactatc agtgcgatta ctaaataaat
    19561 atttccgttt ttcatttgtt tttttgaaga attcataagt cacctctgta ttttatctta
    19621 gacttttttt gaagaaacaa caatcatttt catagagact tcttccataa aaaagtgcta
    19681 tacttaattt tgtattacaa tatgaaggga gttttttcat gagttattca ttaaattgga
    19741 atctagattc tattttttcc ggaggcagtc attctgatgc tttgaaccaa cggatgaagc
    19801 aattggaaga ccaaatgaac gagtattatc accgcgtaac aaaatggtca cctagttctg
    19861 acaatgcaga gcagttaaac gctattcttc aattgcaaga aacgattacg aatggtttta
    19921 cccaatgcag cagctatatt actgcattgc tttctgccaa tgtcaatgat tcagatgcaa
    19981 aggtcttatc aggcaagctt tacgcaatgc tgcctcgtct gcaatcagcc gaaactgtat
    20041 tgtcaaagaa atttgcagaa atatctgaca atgattggaa tcagatgctt tctcaatctt
    20101 cgtttgaaac aattgctttt cgtttaaacg agattcgtcg tgatggctca caattattat
    20161 cagaagctga agaaaacatt ataaatacgc tttctttaga tggtctaaat gcttggagca
    20221 gtcattatga tacgattgta gccagcattt cgattccctt cgaacaagat ggacaagtag
    20281 tcgaattatc tgctggacaa gccttcaata agatgatggg cgatccagat ccaaaagtca
    20341 gagaaacact tttcgcagca tgggaagaag cctggaaaga aaaggcaccc ctcttcacgg
    20401 atactttgaa tcatttagat ggttttagac tttctgacta tgaacttcat ggtgtcacag
    20461 attttctaca aaaaccatta gagtacaatc ggttgaagaa agaaacattg tctgtcatgt
    20521 gggatacaat ccagaaaaac aaacaacccc ttgttaatta cttgactaga aaagctaatc
    20581 ttttcggtaa agaaaaaatg gaatggcaag atcaagatgc acctattatt ttaggcgatt
    20641 tgaaagaaaa aacctttagt tttgacgaag cagcagcatt tattatcgaa aactttaaca
    20701 aattcagtcc aaaaatggct aaatttgccc aatctgcttt tgaaaagagc tggatcgaag
    20761 cagaagatcg tcctgggaaa cgtcctggtg gttattgtac tgagttacca gaaacaaaag
    20821 aatcaaggat ctttatgacc tacagtaatt ctgttaatga ggttgcgaca cttgctcatg
    20881 aactaggaca tgctttccat agcagcacga tgtgggattt accttcttta aatcgcgaat
    20941 atgccatgaa tgtcgcagaa acagcaagta cattcgcaga actgatcgta gccgatgcta
    21001 cattaaaagt cgcaaaaaca aaagaagaaa aaatcaattt gctagataca aaactgcaaa
    21061 atgctttagc tatgttcatg aatatccatt cccgattcat ttttgaaaat cgattctatg
    21121 aagcacggca agaaggatta gtctcagaag ataagatcac agagatgatg gttgaggcgc
    21181 aaaaagaagg ctatcaagga gctttagcta cttaccatcc atatttctgg gctgcaaaat
    21241 tgcatttctt tattgatgac gtaccgtttt ataacttccc atacacattt ggctacctct
    21301 ttagtttggg catctatgct tacgccaacc aaaagggagc aaattttgaa gaagaatata
    21361 tccatttact acgcgatact gcctcaatga cgacggaaga tcttgcgaag aaacatttga
    21421 atgtcgatct tacccaacca gatttttggc aagcaggtat cgacatggtg actgaagata
    21481 tccaaacgtt catggaattg acagaagaat ttatctgatt tctattttat cacaataaaa
    21541 aaagagttag tccagaatgg gctaactctt tttgattaat cattcttaca tgctgacagc
    21601 aaagtcaggg gcaaaccatt gaactgaacc aacttttacg ctttcgcctg gttgaggagc
    21661 atggatatat tgtcctccgc ctaaagcaat cgctacgtgg taagttccgc cttgtgaccc
    21721 ccagaataac aagtctccag cttttgcttg agaaactgaa atttttgttc cagctgattc
    21781 ttgaggaact gtccaaccac cgatgtcacg accagttact tgcatgtaaa catagcgtgt
    21841 gaaccctgag cagtcaaagc cacttggatc tttaccgccc caaacatatg gagtaccaat
    21901 atatttgtaa gcttctgcta cgattgctgc accattcaca cttccagaag gagctggagc
    21961 aggtgttaca ggttttggtt gttcaggtgt tggtgttggc gttggagccg gagtactact
    22021 tcctgtgccg tttcctgtat caacactttg atctgttgat ggtgttgtag gcgcaggtgt
    22081 tgttgattct tctgttgttg aagattcagg cactgttgtt gattcttctg ttgctgaaga
    22141 ttcaggcact gttgttgatt cttctgttgc tgaagattca ggcgctgttg ttgattcttc
    22201 tgttgctgaa gattcaggcg ctgttgattc ttctgttgaa gattcaggtg ttgttgattc
    22261 ttctgttgct gaagattgtg tagctgatga tgattcttca gtcattgaag attgtgtagc
    22321 tgatgattct tctgttgctg agctttgtgc agtagatgaa gcttgagtag cttgtgcttc
    22381 agcctctgct tgttcgcgtg cttctttttc agctttttct tgtgcagctt gttgacgagc
    22441 ttgttctgct agacgttgtt gttcacgaat acgagcttgc tctgcttcag cttctgcttt
    22501 ttgacggttc aagtcagctt tcttgtcttc agcagttgct tgttcagcag ctaatgaagt
    22561 tttcagtacg ttcaaatcag cttgtttaga taaaagatcg cctttttgag attccaaagc
    22621 agcttggttt tcagctaatt cttttagttt agcatcgttt tcagcttttt tgtcttctac
    22681 agctttttta tcttgttttt gttgttccat caagtcgttg tttgctttta ccatcgttgt
    22741 cattgcttga acgcgtccaa tagcatctgc taatgaatca gcattcaata cagcatcgat
    22801 gtagtttgag cttgtgttgc ttacttgtgc ttcgcgtgct tgtttttgga ttgtatcttc
    22861 acgtttttca atacgttctt gtaaatctgc aatgtctttt actaattgtg cagattcttg
    22921 acgcaatgta tcttgttttg ctaataggtc ttgtgcttgt gtgttgattt cagaaacttg
    22981 gctttctaag gcatcgattt gtgattgtgc atctgcttgt tgatttttca agtctgcaat
    23041 cttttgatct tgctgttgta tctgagaatc aaaatcgtcc gccgcggcag cgatcggtga
    23101 tgctacggca gtcaaggtca ttgaacatac cattactgct gatattaaac tctttttcac
    23161 tcttcattcc tccgactggc ttaatttaat aaatatctaa atatatttaa gtctttctta
    23221 acgatataaa aattgtacca tagcagtgtg tcaacgatat aatagttatg ttacaaaaat
    23281 gtttcaaaac aacttttaaa aatgacaaaa ctgttcgttt cagaaaatga tcatgaaaaa
    23341 gccgcgttaa gtctagcatt tcttaagata acaacgattc tttttcgctt tcgacctaaa
    23401 aataaaaaaa gaaattattc atcactgaat aatttcttaa agggaaaaat gaataataca
    23461 aatataatca tgttcaacag cagtgtaggt cctaaaaacc ttgtaataaa atacgtgtta
    23521 tttactactg ctaatttaaa aatcaactgg accaccatcg taaacaattc ataacccgtg
    23581 acaaagatga tcatactgaa aaattcagta aagatgttga catggataac gtctttcatt
    23641 acatacatca accatacaat taaagggaga gctactgcat agataccgat aacgccaata
    23701 tagtaagcgt catagatagc cccgagaaca atcgtggtaa tcagtaaata acgtttttca
    23761 aacgccatac tgcaacataa taatgccaat atcagaaagt gggcatttga catataggtg
    23821 cctttcgacc actcccctag cattcttgtt aaatgaccat ctatcaacat caaaaagaac
    23881 aaaacgattg gcagatagta cttcattgtt tctttcttca acatggacta atcctccgct
    23941 agccgttgga caatcgtcac aactgacacg tcatacattt ctgcataagg tttcacataa
    24001 acttcccgat ccagcccata actatctggt ttcgttttaa taaccgtacc gatcggcaag
    24061 ttagctggag aattccctcc aagaccggat gtctggacaa catccccttc tttgatatcc
    24121 gtatcaccag ttaattgggt caccactaag gcatggagct tttcatcata gtttttaagc
    24181 aaaccaaacg cttcgccatt agccgaagat acccgtactg gaaaatgatt ggagctttca
    24241 ttagaggatg acagtaattc gattttagac gaagccgtat tgacctcgat cactcggcca
    24301 atcaatcctt tttgcgacat gactgccata tttgcttcga taccgtcttt agaacccttg
    24361 tctacgatca gcatgtcctg ccaactgtca ggcgaacgag taatgacatt tgccgttact
    24421 ttttcgtaac tagtcagcgt ctgattcaac tcaagttctt ttttcaacgc ttcgatttct
    24481 ctctcgtaat tcttgttttg ctgtgaaacg tcatcatagc tatcgatttt ttcttttagc
    24541 cgctcatttt cggcataagt tgaaaaaaga ttatggacag aatctactcc atttcgtacc
    24601 caccgtactg gggcagagat tgttttatca accattgcaa cactgtcatt aacgatggat
    24661 tggaagaaat tgggctttcc ttcattcgct cgctgcgcag cggtcacact tagtatcgtc
    24721 accacgatga taacaataac caaggtaata ataatatttt tattaggatt gaacttcttc
    24781 acaaagacac ctcgaattaa ttagactctt gtgttcaatt ttatcactaa atgccgtgaa
    24841 acaaaaggta agttgctcct cttaagcttt tttttagcaa ataatagatt ctgtcatttt
    24901 tagcctgaaa gacaaatttt tttatttgtt tatggatcaa tctttgcttt gaataactgc
    24961 aatcatacca tctgaaaaag aaaattctgc ggtatctgaa agaaattcgt tacttgcata
    25021 agatgtcgga tattctactt ctttattttc caatacatac ttgctgcctt ttaatgtaag
    25081 attcgagacg ggagtcaagc agcaataagc taaatacttc atatctggta tctgtcttac
    25141 tatatgctct ccaggcaaat aataagacaa ataattttga caatctctga taataagctg
    25201 atgggcaaag ggtttgaagc gaggttccat acctaaccag agattggcta aaagatgatc
    25261 caatcttccg cccgtagctc caatcaaagt gacttccgct tgcgggtaac gttcaaatgt
    25321 ttttaagata cctaactgtg tatctgtatc gtctttttct gcaggggaac gggtatactc
    25381 tttggctttt gcaaatactt ccttctgctc ttcgttcgac aatgaatcaa aatcacccac
    25441 tgccagatct actgggtagc ctgcttgaag caggtacagc gcaccacggt ctatcccgac
    25501 ataggcatcg tatttggaca gcagagactg cggccagttt tgcggattac ctccagcagc
    25561 taataaaatt ttcatttagt ctaatgcctc ttttaatcgt tcgatttgtc cgactggatt
    25621 ttcggctcca tagatatatg atcctgcaac gaagacatcc gcccctgcat ctttacagat
    25681 ttttgctgtt tctggaacga ttccgccatc tacttcgatt gcataagtcc aattgttctc
    25741 tctacgtaga tcagcgagtt ccgtaatttt ttctactgtt tcttcaataa agctttgtcc
    25801 gccgaaccca ggattgacag tcatgactag tacttgatca gccaaaggca aaacatgttt
    25861 gatcatggag actggtgttc ctggattgat aactactcca gctttaactc ctaagctttt
    25921 tgtcatttga agtgcccgat gaatatgagg agttgcttcg acgtggacag taatgatatc
    25981 tgctccagct tttgcaaatg ctggaatgta gttttctgga tcaacgatca tcagatggac
    26041 gtctaacggt aatttagtaa caggacggat tgcctgaaca atatttggcc ctaatgtgat
    26101 atttgggaca aatcggccat ccattacatc tacgtggatg taatctgcgc ctcctttttc
    26161 cactaactca atgtctcttt gtaaattggc aaaatctgca ctcaaaatgg atggtgctat
    26221 cttcattctt gtttcctcct attttcgttt accttctttt tttgcataca ttggttttct
    26281 tttttcgatt tcttgtaaga attgcaaata attgtcgtaa cgtgtctgtg cgataccacc
    26341 tgtttctact ctatgtttta cttcgcagcc aggttcttta gcatgcatgc actctctaaa
    26401 tttgcaatgg acagaagctt ctacaaattc aggaaattgt ctcggcagtt ctcctgcttc
    26461 aattgtcaaa aagtcaatcg agctaaatcc tggtgtatct gctactaacc cgccatagat
    26521 aggaatcagt tccacatgac gtgttgtatg ccgtcctctt ccaagtgcat cagaaatctc
    26581 tcctgttgct aaattcagtt ccggtgatat ttgattcagt agcgtcgatt ttcctgctcc
    26641 agactgaccc ataaagaccg atagccgttc tgggaagtaa cggatcagtt ctgtcgtatc
    26701 acccgtatga ttgggtacaa tcaccggata gcctatttta gtataggttt gcttgatctc
    26761 ttcgattttt tctgggtcgt ctgacaagtc ggtctttgtc aaaaaaataa tcggttcgat
    26821 cgactcgtat tccaaggtaa ctagaaagcg gtcaagtaga ttataagaaa atgcaggctc
    26881 tacgatactt gtgacaatga ttccaagatc aacatttgtg actggtgggc gaactaattg
    26941 attttttctc ggcaagattt ccagaagata gccgtctgtt tgattttcac tctcaaagat
    27001 cacctgatct ccgacaagcg gcgtgatttt ccgattccgg aaatttcctc ttcctcttgt
    27061 ttgataggtc tctccgtttg cgtatacata gtaaaaaccg ctgattgctt ttctgatttg
    27121 tcctttgata agtgccacct ccgtttttgt ttattttacc atacgaagag gaaagtaggt
    27181 gttattccat gcaaacttct caaagtatat ggcttgaaaa ttgatcaaaa gagatagaaa
    27241 cacaaaaaag agaatgcaac aaatcaatgt cgcattccct tctgatatat tttattcagc
    27301 acttattttg aactttctga ggtgctagct gatgaacttg tggagctgga agattcttct
    27361 tttgaagagt tgcttgtgct actggagcta cttgagctgc tagaactgtc tgaagagtct
    27421 ttttcagaac tagaagacgc tggatctggt cctttagagt aaattacgtt cacaaccatt
    27481 cctttggtaa ttgtcgttcc tgcccctggt actgtacgaa tcactttatc tttatccacc
    27541 gtgtttgaat attcggaagt ctcatgcccg atatattctg caccgacact agctaaataa
    27601 ctttgtgctt cgctttttgt atagccggag atatcattca atttaggctc tgttccttgg
    27661 cttacgacaa aattgatgta agtttcttct gctacaactt cacccgaggc tggatcctga
    27721 ctgatgatcg aacctgatgg tacagtgtcg ctatagtcat aagtagcact aatattattg
    27781 atattgaatc cttgtgtaac aagccaatta cgaacagttt cgtagttttc tcctacatga
    27841 ctactcaaag tcactgtttc tggtcctttg ctgactgtga aagtaatcgt gtctgtttta
    27901 ggatcgactt ctgtatcttc tgatggagat tgcgaaatga tcattccgct ttctacctct
    27961 gaactgaatt cctcttcttt ttgaatcaag tcagcagaaa accctaattc tttcaaactt
    28021 gcttcggcat cttcataact tttaccactg taatcatcta atttgatttt tttcgtgccg
    28081 ctactaatat acagagtgac ttcgcgtttt tgcttgactg tcgttcccgc tgccgggtct
    28141 gtcttcacta cattaccttc ttcgattttg tcatctgcaa tttcttctgt ttttgttgct
    28201 acttctaatt tcgcttttgc gagtgtttct ctagcagaag cttcagaaag atcagaaaca
    28261 tcaggaacaa caacttctcc tcgcccgcca gaagcaaaga atacagctgt tccgattcct
    28321 aaaacagcaa gaatagctaa taggatcagc caccattttt tctgtccttt ctttttagga
    28381 tcaggcagaa cttcttcctc ctctggtaag gtttcgtttt ctggcttgtc ttttggcaga
    28441 ggcatagatt tgaatgaatc aggcattggt gcatcttccg taaggggtgt gataactttt
    28501 gtttcattat ccatcacatg aggctgccat ttttcttcgt ttgcacgagc tggtgataat
    28561 actgtagaaa gatcatcaga catttcctcg gctgacttat aacggtctgc aggttcttta
    28621 gccgttgccc gtaaaatcac gttttccagt gcttgtggca catttggatc aagtgctttt
    28681 atagatggaa gatcatcttg aaaatgtttt aaagcaattg ttacagcaga ttcaccatca
    28741 aacggtacgc tacccgtcaa catctcataa aggatgatcc ctaaagcata aatatctgac
    28801 tgcttcgttg ccatacttcc tctggcctgc tcaggggaca gataatgaac agatcctagc
    28861 atcgtattcg tctgcgtgat cgatgtttca gataaagcga tagcaatccc gaaatccgtg
    28921 atttttacaa caccttcatt atcgatcagt acgttttggg gtttcaaatc tcggtggatg
    28981 atttgatggt tatgagctaa agaaatagca gaaaggatct gctgcataat atttactgct
    29041 gtttcataag gaacaggata atgtgtttgg atatatcgtt tcaagtccat tcctttgacg
    29101 tattccatca ccagatactg catgttgtct tcttcaccaa catcgtaaac actaacaatg
    29161 tttggatgaa cgagttctgt tgctgctaaa gcttctctct gaaatcgtcg aatcgcagtc
    29221 tggtcatttt gaaagtcaaa tcgtaagact ttaactgcta catcacgatc caagattaaa
    29281 tcgtgcgcca aaaatacatt tgccatacca ccgctaccga tatttccagt gatttgataa
    29341 cggccattta attttttgcc caactcgatc atacactgtc ctccttatat tcgataagaa
    29401 gaacggtgat attatccgct ccgcctgctt cattggcacg atcaatcagc tcagttactt
    29461 tatgagacaa tgggccttct tgattgatga tcgttcctat ttcttcttca gacagcatgt
    29521 tcgtcaagcc atctgaacat aacagcagtc gatcttttag ttgccaaaga tgagtagaaa
    29581 catctacttc gactgttcca ggcatgccga cagaacgtgt taaaatattt ttccttggat
    29641 ggttcactgc catttcttcg ctgatttctc ctgatttgac tagttcattg actaaagaat
    29701 gatcctccgt caattgcatg attttatctt cacgaatcaa ataagctctg ctatccccaa
    29761 cattggcaat cgtgaaccag tcatctgaca gagcggccgc tacgattgtt gttcccatac
    29821 cgttatattc cggctgttct tgtccacgct gataaatcag catattttct tcttgaatcg
    29881 attgaatgaa ccattgggcg attttttcat cgtcaacagc ttcttgtttt tcccaagctt
    29941 cgcctagatt cgttaccgcc atctgactgg caatgtctcc ggcacgatga ccacccatcc
    30001 cgtctgctaa tacagcaagc ttgagacctg aacggctggt aaatacactg gcatagtctt
    30061 gatttgtgtt acgtcgtctt cctacatctg attgatattc aatctgcatt ttttcacctc
    30121 attacttttt gcgcaaacaa caaatgaaaa atccatctgt gtaaaactga tgcggataca
    30181 aagtcaacat tttctcttcg attgatgatt gtagcatagg attggtcaaa acatcaatcc
    30241 tctcaaaatc agggtgtctt tccaagaaag cagaaacaac gtcttggttt tcttctttta
    30301 agatcgtaca agtgctataa gtcagaatac cggaagactt taaagttggt gcgcagcttt
    30361 ctaaaatttc caattggatt tttggtaaat tcgctaattc atccggctgt tttttataac
    30421 gaatatctgg ttttcgccga agcagcccaa gacctgaaca aggcgcgtct actaaaatcc
    30481 gatcaaactg ctctgcttcg aactgtttac tgacttctct agcatccatc ttttgagcgt
    30541 agacagcaga agagacacct agacgtgctg cattatcttc aatgagtttg actttgtgtt
    30601 cgtgtacatc taaagcgacc acttctccac cgcatgacgg atctaaaaaa gaagcaatat
    30661 gcgtcgtctt acctccgggt gctgcacagg catctaagac atgatgttca ggctcgattt
    30721 gcatagacgg cgctacaagc atcgaacttt catcttggat cgtcatgacg ccatcgtgga
    30781 acaatgagct agaagctaaa tgcccttttt tcgctactat accgtatgga ctgacttggc
    30841 ttttttcagc tgtgatctct tcttcttgca aaacagtgat ggcctgatct ctggaaagtc
    30901 ggcgtgtatc tactcgggca ctagcatggc taggctgata tagcgataaa ccgagctttt
    30961 cagtttcttc gtatccgatt tgatccacca gtcgtttcgt catccataga ggcaggctga
    31021 ttttgacaga aagtctttcg ataggatcag caatgccttg cagatctggt gctccttgac
    31081 gctgataatt cctcaagaca ccatttacaa atttccctgt cccaggattt ccttttactt
    31141 ttgcgatttc gaccgcctcg tttaaaatag catgtgcagg gactttatct aaaaattcta
    31201 attggtatac agaaagataa agaagcatct tgacccagtc atccactttt tttgctttag
    31261 cgatcaatgg tgcaacgtaa aattccaata gtcgctgatg gctaatcgta ccatagatca
    31321 attctgtaaa taaacccaca tctttttgat ttagctcgct ttgtttgatc atctcattca
    31381 acaaaagatt cgagtaggcg ccacccttgt tcactcgctc taatgtcagt aatgccatat
    31441 atcgtactga ttttttcatt tttttaggga tttttttcat tatttcacct gctgaccaat
    31501 ctgaacttgc tgaccgcttc cgttaaggaa atcatgtacg gcctgttttc ctttaccagc
    31561 tggctgtaat tcattgatgg ctaagacggt ctgtttaccg caagcaatcc atagattttt
    31621 tttgtctttc cggatgatcg taccaggtgc tttgtctgta gtttctgcca atacttcaac
    31681 cgataataat ttccagcgta tgccctcata ggtcgtgaat gcaattggcc aaggacgcat
    31741 tccgcgtact tggttgtcaa tttcttctgc agttttatgc caatcgattg cttcttgttc
    31801 ccgtgtgatg tttggcgaaa aagttgcttc tgactcgtct tgaggtactg gtttttgtcc
    31861 atccaaaata ttgggcaatg tttctagcaa tagctttctt cctaaaaggc taagcttttc
    31921 aaacatcgtc cctacatcat cttgtttagt gattggtatg ctttcttgcg catagattcc
    31981 gcctgcatcc atttttttga tcatttccat gatcgttaca ccagtttctt tctccccatt
    32041 catgatggca taatgaacag gcgcacctcc ccgatattta ggaagtaaag aagcatggac
    32101 attgattgcc cctaattttg gtacttgcaa taacttttca ggcaagaatt ggccaaatgc
    32161 cgcagtgacg atcacatcag gcaccaattc aatgatttct tccatttctg gcgaaccgga
    32221 gattttctct ggctgcagca cacggatacc gtgcttcaga gccgcttttt ttaccggtgt
    32281 cggcgtaatt actcgtttcc gcccaactgg acggtcgggc tgtgttacca ctgcctggat
    32341 ctcgtaacct gattctatca gcccttcaag aatcggcaca gaaaaagcag gtgtgcccat
    32401 aaatactagc ttagtcatca agatgttcct ccatatattc ttcaagttct tcttccggga
    32461 tctgctgtat cattttctca atgaacaaga taccgtctaa gtgatcgatt tcatgctgga
    32521 aggcacgggc taaatagcca aaagccgtca cttctatctc ttctccgtct cgatcataat
    32581 aacgcacggt cacttcatct gctcgcttga ccgttccata gacatgcgga atactgagac
    32641 aaccttctac gtcaaggctt tcaccttttg cttcgatgat ttctgggtta atcagttcaa
    32701 atttctcgcc ttcatctact tcgatcacag caattctctt gttttgtcct acttgaggag
    32761 cagcgatccc tattccgtca ttcgcaatca ttgtctcata taaattgtct aataaagcga
    32821 ttgtctcgtc cgtgattaca tcgattggct gagcagttct ttttaattta tcattagggt
    32881 ggatcaatat aggatagcgc ataatttggc cctttcttaa ataaagttta atggttcgtt
    32941 gtcaattgcc acaaaaagac cttgtcgctg ttctttctgg cttccattca aaatttcatc
    33001 caaaacatga ggaagattcg gttcatgttt atatttgatg attagttgat aataatatcg
    33061 gtttttcact ctcaaaatca tgctgggcgt tggccctaat agcaagcttt gagggcttaa
    33121 cacttctttt agctgattgg caatctgaaa gattttcttt gccgctactt gttcttcagg
    33181 atggctggcg gttattttca cagtaaagta ataaggagga tagcctccac gatgccgtac
    33241 atgcatttcc tgctgataaa actgctcata attttgtttt ttagccaatt ctatggcgta
    33301 atgttgagga ttgaatgttt ggatcacgac ttcgcctgct ttttccgctc gccctgctcg
    33361 tcctgaaact tgggtaagca attggaatgt atgctcacta gaacgaaaat ccggtaaatt
    33421 cagggaggta tcagcattca acaccccaac taatgtcact tctggaaagt ccaaaccttt
    33481 agcaatcatt tgtgtcccta ataatatatc tgcttctttt gcaccgaatt tttgtaaaat
    33541 ctgttcgtgt gccccttttc ttcgagtggt atctacatcc atacgcaaaa tcctagcttg
    33601 cggataaagt tctcggagtt cctcttctac tttttgagta cctgttccat agtagcggat
    33661 tttattgccg ccacaattag gacagcgatt gggaataggt tcttcatgtc cgcaataatg
    33721 gcagcgcatt gaacgtgtat ccatatgcag tgtaagagag atatcgcaat taggacatgg
    33781 aagaacaaat ccacagtccc tacacatcac aaaagacgaa tatcctcgac gattgagcag
    33841 taggacagtt tgttcttttt tgtctaatcg attttgtatt ttttcctgca aattagcaga
    33901 aaaagtagag gtccgatgat tctgaaactc ttcacgcata tctacgatct caatagctgg
    33961 aagctgtgca gctgctttgg ctcggtggtt taactgcaat aactgataaa cgcctttttg
    34021 agctcgtgct cttgattcta aagagggcgt cgcgcttcct agaactattg ggcaatgatg
    34081 atatttactg cgccaaattg ctagatctct cgcatgataa cgcggtgtct catcttgttt
    34141 gtaacttgat tcatgttctt catcgataat gatcagcccg atatttttca gcggggcaaa
    34201 aatagcagat cgtgcgccta ctactacttg cgcttcttcc cgctcaatct tgcgccattc
    34261 atcatatttt tcaccttggg aaagtccgct atgtaagacc gcaactgatt ctccgaagcg
    34321 tcctttgaat cgctcgacca tttgcggagt caatgcgatt tcaggcacta acataatagc
    34381 cgttttattt tctgacaaaa catcagcgat tgcctgaaga tatacttctg ttttcccgct
    34441 gccagtgatt ccctccagca aaaagacctg atctttttgc tgctgacctg ctgtgatgat
    34501 ttgttcgaca gcattttttt gttctgcatt cagttccaat gccgtcgttt ggtcaaatac
    34561 tcggtcttta taagggtcgc gataacgttc tgattctata aactccagcc agcccttttc
    34621 ttttccttga ttcaagacag cagtactgat tcctttattt ttgaaataag caattggttc
    34681 ttcttggctc agttcagata aacacaaaag aagttctttt tgtttcttgg cattttgccg
    34741 taattttgcc cattcttctt cgatttgctt ttcagttaac aaagaacgga ccaaacgaat
    34801 cgtttttact ttatttcgtg tatggacttc atatttgata tctactaatt gctcttggcg
    34861 ccagcgaatc aattgagaaa ggctgccttc ttcttctgct tccttccagg aaatcacttc
    34921 tcgctgatca aacaatacct gaacttctgg tgtgtctgcc aaaggataga tcaatttatc
    34981 atattctgct ttcatcacac tcggaagcat cgtttgtaga caagtgattt tgaatgcgta
    35041 agtaattttt ttcatataat cagctaatga aagtaattct tcattgacca ccggagcaag
    35101 gtctagcaaa cgaataactg ccttcaaaga tggattagct gattcttctg atttttggat
    35161 agccataaca aatccttgga catgacgatt gccattacca aaagggactt ctactcgcat
    35221 cccgacttgc aaagctgttt ccatttcaga gggaaccaaa taagtgaatg gctgatcggt
    35281 ctgcatagta ggtacgtcca caatgacttc agctgttaat gtcatggttc gtttcctcct
    35341 ttttttgtct tttcttatca agcgacaaaa agttccttgc ctattttact agagatcgat
    35401 ggatttgtct tatcttctgc caaatcctaa caaaaaatta acagcagata agaaaaaaaa
    35461 gaactgtgtc aaaaagttca gacacagcct tttctaccag acaataagtg gcgaatgttt
    35521 gaaaaggaaa cagctgggtt tgcttttctt tttagatgtt cgctctattt ttctttatta
    35581 cattttttgc tcttcacgga tacgtgattc caattcgtgt tgttcacgct ctttttgtgc
    35641 ttgacgttcc tcttcttcca tcttcaaacg ttcacgtttt aattcaggat gtggatcatt
    35701 gactacattt cctgcatcga tttcttctaa agcttgaccg acgcttttta ctgaatcaaa
    35761 agaatctaga gtaggctgtg cacttgcatc caattcatgc gcgcgtttac ttgctaaaat
    35821 cactagagaa tattttgaat ttacacgatc taatagtgag tcaattgaag gttttaacat
    35881 cataagacta catctccttt aacattttaa tatatttacc gattacgcga tctactcgaa
    35941 aatgttcact ggcaataatg tttttgattc gttcaactgc tagcggcact tcatcattta
    36001 caacagcata gtcgtaaaga gccatcattt cgatttcttc ttttgcaact ttcattcgtt
    36061 tttcgattac ttcatctgca tctgttcctc ggcctacgat ccgtgatttt aattccgcta
    36121 aatctggcgg agtcaaaaag atgaatacac catcgggcac tttttctttg acttgttttg
    36181 ctccttggac ttcaatctcc aagaaaacat cttttccttc atccaatgtc ttgttgacat
    36241 atgacaaagg agttccataa taattcccga catattctgc gtattccagc atctctccag
    36301 cttcgatcat tgattcaaac tcttctttgc ttcggaagaa ataatcaacg ccttcgacct
    36361 ccccttcacg cattttacgg gtagtcattg agatcgaata ctgaaaatca ttatcctcac
    36421 tctcaaaaat tgcttttcgt actgtgcctt ttcctacacc ggatggacca gacaacacga
    36481 ttaataatcc acgctctgac atgacgacgt cctttcaact agtctttttg tacttatcgt
    36541 ttattttgca ctttttttct gcaactttca aggttatctt taattatagc tagaaattgg
    36601 taaaaaagct accctgtacc aagaggtcgt aaaaaagctg tcctgcatca agtaataaga
    36661 acagccaaac ataaacagct tctcatgttt ttcgtttggt tggggttatt atcgcagatg
    36721 caagcttttg aacgccgtgt ataagaggtc gtgaagaagc cgattagttt cgagtatgaa
    36781 gaaaaatttt gtgaaaatag tttctcacat ttttgcataa atttggctta ataccgagaa
    36841 aatggttttt gaacatcact tattcaaaat aaaaaaagat tgtgacacga attttgtcac
    36901 aacctttaaa tgtattccta gagcaaagaa gctaagcgaa gaaatccaat tctgtttatt
    36961 tattgtattt ctttgttcag catccttgct aaaagcccat attggacaaa gtattcacta
    37021 cacccatgat ctcatctttt cgagaacgtg ctttttctgt cggatcttct acctcttctg
    37081 tgtcaattcg aacaataaat ttccgattca tcgtcacgta agcaccgtcg ttgatcaaaa
    37141 taaacagttc gttctcatta tagtctgcac aggcatcttt ccatacgtca aagccgtttt
    37201 tttcagaagg gatatcccgt ttaaccacag tttttccact taattcgtgg aacgttacgt
    37261 cataatattt cattatctct acctctttcg tcattcaata ttttgtactt gttctctgat
    37321 tttttctaag atcgtcttag actggacgac ttgattttta atttcgattg catttgattt
    37381 ggaaccgatc gtgttgattt cccgattcat ttcttggatc aggaaatcta attcccgacc
    37441 tacaggacat tgtgcttcga taagttcatc tagtttttgt acatggatcg ccagacgatc
    37501 cagttcctcg tgaatatctc cgcgttccaa caagatggct agctccgtca acaaccgttg
    37561 ctcatcgact ttcgctccaa gaaattcctc cagctttttt tgatacctta tttgaaattc
    37621 cttttcatag atttctacaa aactatcgag tttttttagt acttctttga attcttcagt
    37681 attctttttc aacactaaag ctaaagcttg accttctttt tctcgactcg cctcaataga
    37741 atcaagtgcg cttttcaatg tatctagagc taactgtgcc aattcttcca tgctctcttc
    37801 cggtttttct tctaccgtta cgaagttttc gtccagcacg aagcgttccg ccatccgact
    37861 aattgacact tgattttgac taccgtatcg ttgttgaatt ccgtctgtca attgagtgat
    37921 aagtgaatca atcaattccc agttgatgac gatctgtttt tcgtttttgc caatataagc
    37981 tagattcaca aatacttcga ttcgaccgcg gcttaactgc tgtttaataa gctgacgaaa
    38041 atcgttttca acaaagttca ggattttagg acttcgtatt tgaacatcta gaaaacgctg
    38101 gttgacactt tttatttcaa tatctaactg atacgctttt gtttcgcgga tggctttgcc
    38161 aaaaccagtc atacttttca ttcgacaggg tccttcctca ttacatattt tctattgctt
    38221 tttgcagttg ttcaaactct tcgttcgtca atgtcaggcc tttccccatt ttttcatggt
    38281 ctggcgccca atctcgaaga tcgaattttg gcgggcgacc attccagctg actaggttca
    38341 gttctttacg ccaccccttg ttgttttctg acaatatagc gatttcttct acgatttcat
    38401 atgaaaattc ttgtgccatg gttgttcctc cttgagataa tataaatata ggatcagtaa
    38461 tgtgtcaact ggttgcttaa gcaattcatg ctgcaaacga tccttgcgtt ttccttgtag
    38521 cagataatca aaataaacag ttttcgtgcg ctcattttgt attttttgct ggtattttcg
    38581 ataccttcgc cattcttcag tacttaattg accaatttgt gccaataaat catccggcat
    38641 actttttata tcttgttggc agatcatttg aatcatcatg tctacttgat agatttcatt
    38701 gggcagtaaa aaatgttttt ttgctaaaag ttctagcatc gccaatccgc atgtctctgg
    38761 tacgaccctg ttcttatata aattacgcag tttttgccaa aattttatgc ttttgaaaaa
    38821 agataattct aactgaattt cttttgttaa gtaagacaaa aatcctcgcc agtatgttct
    38881 tgcaggttta acagaatgaa atgtatacca gttcccatag taatagccta atcgtttttt
    38941 catttccaga tagcctaatt gtaaatttaa ttggttcctt tggctatcaa aaagcagaaa
    39001 gctgcctagt gtccacaatg tttgacattt ccaatggata aaaatgtctg gcatacgaat
    39061 ttttttcgct gggcctggcc cttgcacatc tacgacaaat gcctctgttg ctcctttgtt
    39121 aatcgcaata tcaataggta ttttgttgcg atacccaccg tctgcatatt tattttttcc
    39181 gatctttcga taagccatag cgggataaaa tgaagcagag gcgagtatcc aatctggtat
    39241 ttcttctgtt tttgtctgtt ggatatcagt cactacttcc gctagttctg gcagtttagt
    39301 agacaccgta tataaacgta ttttgggatt atgtttgagt aaggacaaat ccaaattgtc
    39361 tttgaccatc ttttccaatg gagcagaaga aaccccgcct tcaattagtg ctgttttcgt
    39421 catttggcgt ctttctcgat agaagcgttc acgaaggtct tcaactgcag ccatttctgg
    39481 taacgcaagc acttgtcttg tcgacagctt tttccataaa ttcacagctt ttttcatatc
    39541 tccttgtaaa atcaatgcgc cgttcaacgc acctaccgag gtacctgtga taatctcaaa
    39601 gttgatatca tgttccttca gcgcctgcca cacaccaatc tgataagcac cgtgcgctcc
    39661 tcctccacct aaaacaagtg ctgtatgata gaccagcttt ttatttaatt gtgtggttcc
    39721 ttgatagccg tttttatgaa aaatacgcaa ccattcttct gatagaggag attgaaataa
    39781 aaaacaacag ttttctttaa taaaaaaacg agcgcaagct tccatatacc gaaaaaattt
    39841 tttccattca aaagtttcac cttcaatcaa cagattatta aaaataacat ctcctctact
    39901 tttagcaata gtaaaataga cggtatattt tttcgatact gctttaaaag cacattgctt
    39961 ttttgcagaa cgccaaaaag cgtctctact ttcttgatga ctttgaaaaa atggaagggg
    40021 ttgttttgct tgctggatag gcgaatacag cgacattttc tgtgaaaagt aaactttttt
    40081 tatctccatc gcgctctcct ttttcttcaa gtataccagt acgtcataaa aaggtcatga
    40141 gtctcgtatt cgactttgta gattccataa gaaaacaatt cataaaacct cctttaaata
    40201 aaaaaataca aaaatcacaa aaattaattg aaaatgagag tgttttcaat gaaaacgtga
    40261 ttttgtaatg gaattttaaa ataaaagaaa caagcttttt taatgcaaat gttgtaaaat
    40321 aaaagagatt aagaggaaat aagaggagga acatgatgac tgagtcagtt tatgtacata
    40381 ttgataccac aagtaatgct gttttaacaa aagggctgac tgctactgac tttgcaaaca
    40441 gtatcgtgca ttttcctcaa aacttgctct tgcttgatcc atctgcttct gctggagaat
    40501 atgaatccca tacagggctg aaagtaatcc gtggtacaga aaatattcag cgtttctttt
    40561 cctcttctcg taacaggcgt ggattcgatg aattgaagtg gatcgacttt actgatttaa
    40621 cgatgctgaa agaattgaca cctttagaaa tttcagaatt attatatttt ggtcatatga
    40681 aaacacatct tcattcgccg tttttttata aactacagaa taattttgta tacttcgatc
    40741 ttaatgatca gctgaatcgg atttattatc gctatttaga tgaattttat aggatttttg
    40801 ctagtaaatt aagttcgatc ttgtcagata agatcaatgt aaaaaaatct tttttcaaaa
    40861 aagaaacacc tgttgaaaaa atcagtgtgg aattattgaa agaaatgcgt aatttgatgc
    40921 aggaagggat cattttttct tttgatcaag ttggtttgat cgatggtaaa tatcgtatcc
    40981 caatctatgt gattgaagat tatatttgga aattaaaagg cacccggtat cgagaagagg
    41041 aaatcatcgc tactttaatc tactctgtca ccgatcgaca ctggcaattg gtagaagatg
    41101 ccgatctttc ttatctgcca ttgaagtaga tctatttctt atcaaaaaat aaaaaggaaa
    41161 caagaaacta ggacatgatt ctgtctcaag tttcttgctt cttttttatt tacagctcct
    41221 gtcgtttatc tggcacaaaa atcgaaggag tcgtttgttg gatccctaat aaaacgatga
    41281 tcgttacaat agctgtactc aacccgctgg caccattcat tacgaaagaa aagagccaag
    41341 gattcatccc ccatagtgca tacgctcccc agaaaaccac tcctgcaata aaatgccaaa
    41401 aatatctgga aagtgcgcca atcaatgcac caaacacgat gctttttcta atttgtttcg
    41461 tattatcgtc tacatagcct ttttggacat ttgaagccat gagccctgct gcgcctgcaa
    41521 aagtataggc aatcgggtac tcaatcaaca cttgaacgac acttaaataa taaacttgtc
    41581 ccaacggaaa atgtaggatg ccccaaatga aacctgccag catccctgcc tttgtccctc
    41641 ttcttaacgc aaaaagagtc aatggaatct gccctaatga aatagaaaaa ctgctgccga
    41701 tctggatcgg aatcaacgac aacaccatcg ctaacgctgc aacaatcgtt ccttctaccc
    41761 aaactctatt ttttcccata aaaaaacttc ctcctttgaa taaaaaagtc acaaaggagg
    41821 aagcagcttt cgaaaatagt caaacagctg tttattcaca attcctacgc tcgcataacc
    41881 gatacaggta cgaagggttt agaaatagat tcaatctcag cccaattgga ctcccctttg
    41941 tgagtttatt tattatttat tttttgtttc aaacaatgga ctaactggtt tgttttcatg
    42001 gatacgtttg attgcatctc ccattagtcc gccaacactt acttcatcga ttttgtcgat
    42061 tttacgatct tctggaagat agatcgagtc tgtaacaact agacgctcga tagctgaatc
    42121 ttctattctt tgtaaagcag ggccggataa tactggatgt gtacatgaag catacacact
    42181 agttgcacca gcttctttca aagcattggc agccaatgtg atcgtacctg ctgtatcgat
    42241 catatcatcg atcagtacac aagttttgcc tttgacttgt ccaatgatat tcattacttc
    42301 tgcaacattt gcttttggac ggcgtttgtc tatgatcgca atcggagatt tcaaaaattc
    42361 cgctaattta cgtgcccgtg tgacaccacc atggtctggt gaaacaacga caacatcatc
    42421 gcctttgatt cctttttcta agaaataatt ggcaataagt ggcgcaccca tcaaatgatc
    42481 gactggaata tcaaagaaac cttggatttg gactgcatga aggtccaaag tcagcattct
    42541 tgttgcacca gctttttgaa tcatgtttgc tactaatttc gctgtgattg gttcccgtgc
    42601 acgtgcttta cggtcttgac gcgcatagcc ataataaggc atgactacgt tgattgtttt
    42661 tgcacttgct cgtttcagtg cgtcaatcat gatcagcaac tccatcaagt tatcattgac
    42721 tgggctgctc gttgattgaa taacatagac atgcgatcca cgaatacttt cttcaatgtt
    42781 tacttgaatt tctccatcac taaattgagt aacagatgat tttcctaact cgacgccgac
    42841 tgctgctgca attttctctg ctaatgggcg attggaattt aacgcaaaaa tttttaatct
    42901 tgggtcaaag taatgttttg acatggggac ctccactttc tttttcacaa ctctagaatt
    42961 ctacctaata aatagtagtc atatttttta aataaatcaa gtgattttgc aaaagtctta
    43021 gtttagataa gggagttttt ttgcatatcc ttctttattg acttgtcttg ctctcgcaat
    43081 tgccagcgca tattctggaa tattgtctgt aattgttgac ccggcagcaa tcgaactgtt
    43141 tttagctacc tcgacaggtc cgatgatatt cgtgctagaa ccaataaagc tgtggtcgcc
    43201 gacagtcgta tgatgtttgt tctttccatc atagttgacg aagacaacac cacatccgac
    43261 attgatttct tctcccagcg tagcatcacc aacataagtc aagtgaccga ctttagttcc
    43321 tttgccgatt tgagcatttt tcacttccac aaagtttccg atatggacgt tttcgccaat
    43381 ttcagctttt ggtcgtaaat gtgcataagg tcccacatct gcatgtttct tgacatggct
    43441 gctttcaatg actgagtttt caatgacgac gtgatcctca atcctactgt ctacgatttt
    43501 tgaatgtgac ccgatgacac aatcagaacc aatgactgta tttccttgga tttgtacacc
    43561 tgcttcgatc aatgtgtctg gaccgatttc tacgcctgca tcgatgtatg tcgttgctga
    43621 atcgacaaag cttacgccat tgaccatatg catctggtta attcgctttc tcatgatctc
    43681 atttgcttta gctaacgcga tccgatcatt gactcccatt gattcttcaa aatcatccgt
    43741 ttgataagca gcaactgtat ggccttcttc ttttaagatt tcgatgatat ctgttagata
    43801 gtattcacct tgagcattgt ttgttccaac tttgcttaac gcatcgaaca aagcttcgtt
    43861 gtcaaaacag taagttcctg tattgatttc ttgtacaaga gcttcttctg gtgttgcatc
    43921 tttttgttcc acgatttttt ctacgatccc aatatggtca cggatgattc gtccatatcc
    43981 tgttgggttt tctgcttgtg ccgtcaagat cgttgcactt gcattttttc cttggtgata
    44041 ttcaaaaaga ttgttcagcg tttcagtagt caataatggt gtatcgccac tgataactaa
    44101 cgttgttcct ttttttcctt ccaagaaaga agcagcttgc aaaacagcat gtcctgttcc
    44161 caattgttct gcttgtaacg catatttact gcgttctcct agttgtgctt taacttgttc
    44221 tgctccgtgt ccaacgatcg taatcacttc atctggtttc gtttcactga cccgattaat
    44281 gatatgttct accataggtt taccagaaac gggatgtaaa actttatata attttgactt
    44341 catgcgggta cctttccctg ccgccaaaat aattgcataa cgtgcgtcca actgcgtcac
    44401 tcctaaatat agtttgctga tttcagttta tcccagtata cccatttttt caacaaaaca
    44461 agtgattttc ttttatccca gcgttttctg gataaaaaaa tagatagacc ataacgggcc
    44521 tatctatttg gtaaccggtg ctctcctgcc aactcctcgg tcacaagtca cactactcat
    44581 aaaacatgaa aagctgtttt ttgaaatcgt ttcttgtgac ttagagttaa acggtaggct
    44641 tacaacctct tcattaaacg tttttcaatc agctgacctt cggtattagc tcaataaacg
    44701 gcaaaatatg aggagcaatt tttccgttta ttgagctaat actcaggaca ggacgctgat
    44761 ttcacaacct ctagataaac ggtgttcaaa agccagtttc tcggtattaa gccaaattta
    44821 tg
//
LOCUS       DBNICI010000006        37122 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.006, whole genome shotgun sequence.
ACCESSION   DBNICI010000006 DBNICI010000000
VERSION     DBNICI010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 37122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.006"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(147..2300)
                     /locus_tag="AC1ZCC_000276"
     CDS             complement(147..2300)
                     /locus_tag="AC1ZCC_000276"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIQ1628564.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2594..3442)
                     /locus_tag="AC1ZCC_000277"
     CDS             complement(2594..3442)
                     /locus_tag="AC1ZCC_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIQ1628565.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3666..5606)
                     /gene="thrS"
                     /locus_tag="AC1ZCC_000278"
     CDS             complement(3666..5606)
                     /gene="thrS"
                     /locus_tag="AC1ZCC_000278"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIQ1628566.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDSDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5943..6122)
                     /locus_tag="AC1ZCC_000279"
     CDS             complement(5943..6122)
                     /locus_tag="AC1ZCC_000279"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HIQ1628567.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6169..7992)
                     /locus_tag="AC1ZCC_000280"
     CDS             complement(6169..7992)
                     /locus_tag="AC1ZCC_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIQ1628568.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8270..10549
                     /gene="helD"
                     /locus_tag="AC1ZCC_000281"
     CDS             8270..10549
                     /gene="helD"
                     /locus_tag="AC1ZCC_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HIQ1628569.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10642..11289)
                     /locus_tag="AC1ZCC_000282"
     CDS             complement(10642..11289)
                     /locus_tag="AC1ZCC_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628570.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11468..12187)
                     /locus_tag="AC1ZCC_000283"
     CDS             complement(11468..12187)
                     /locus_tag="AC1ZCC_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIQ1628571.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12318..13469)
                     /locus_tag="AC1ZCC_000284"
     CDS             complement(12318..13469)
                     /locus_tag="AC1ZCC_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HIQ1628572.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13686..14426)
                     /locus_tag="AC1ZCC_000285"
     CDS             complement(13686..14426)
                     /locus_tag="AC1ZCC_000285"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HIQ1628573.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14430..14753)
                     /gene="rsfS"
                     /locus_tag="AC1ZCC_000286"
     CDS             complement(14430..14753)
                     /gene="rsfS"
                     /locus_tag="AC1ZCC_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIQ1628574.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14810..15403)
                     /gene="yqeK"
                     /locus_tag="AC1ZCC_000287"
     CDS             complement(14810..15403)
                     /gene="yqeK"
                     /locus_tag="AC1ZCC_000287"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HIQ1628575.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15387..16037)
                     /locus_tag="AC1ZCC_000288"
     CDS             complement(15387..16037)
                     /locus_tag="AC1ZCC_000288"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HIQ1628576.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16047..16358)
                     /gene="yhbY"
                     /locus_tag="AC1ZCC_000289"
     CDS             complement(16047..16358)
                     /gene="yhbY"
                     /locus_tag="AC1ZCC_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HIQ1628577.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16374..17483)
                     /gene="yqeH"
                     /locus_tag="AC1ZCC_000290"
     CDS             complement(16374..17483)
                     /gene="yqeH"
                     /locus_tag="AC1ZCC_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HIQ1628578.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17480..18007)
                     /locus_tag="AC1ZCC_000291"
     CDS             complement(17480..18007)
                     /locus_tag="AC1ZCC_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HIQ1628579.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18187..18972)
                     /locus_tag="AC1ZCC_000292"
     CDS             complement(18187..18972)
                     /locus_tag="AC1ZCC_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HIQ1628580.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18965..19834)
                     /gene="accD"
                     /locus_tag="AC1ZCC_000293"
     CDS             complement(18965..19834)
                     /gene="accD"
                     /locus_tag="AC1ZCC_000293"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HIQ1628581.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19835..21214)
                     /locus_tag="AC1ZCC_000294"
     CDS             complement(19835..21214)
                     /locus_tag="AC1ZCC_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIQ1628582.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21222..21641)
                     /gene="fabZ"
                     /locus_tag="AC1ZCC_000295"
     CDS             complement(21222..21641)
                     /gene="fabZ"
                     /locus_tag="AC1ZCC_000295"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HIQ1628583.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21666..22142)
                     /gene="accB"
                     /locus_tag="AC1ZCC_000296"
     CDS             complement(21666..22142)
                     /gene="accB"
                     /locus_tag="AC1ZCC_000296"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIQ1628584.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22145..23380)
                     /gene="fabF"
                     /locus_tag="AC1ZCC_000297"
     CDS             complement(22145..23380)
                     /gene="fabF"
                     /locus_tag="AC1ZCC_000297"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIQ1628585.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23399..24136)
                     /gene="fabG"
                     /locus_tag="AC1ZCC_000298"
     CDS             complement(23399..24136)
                     /gene="fabG"
                     /locus_tag="AC1ZCC_000298"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HIQ1628586.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24148..25065)
                     /gene="fabD"
                     /locus_tag="AC1ZCC_000299"
     CDS             complement(24148..25065)
                     /gene="fabD"
                     /locus_tag="AC1ZCC_000299"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIQ1628587.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25172..25396)
                     /locus_tag="AC1ZCC_000300"
     CDS             complement(25172..25396)
                     /locus_tag="AC1ZCC_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIQ1628588.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25449..26411)
                     /locus_tag="AC1ZCC_000301"
     CDS             complement(25449..26411)
                     /locus_tag="AC1ZCC_000301"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HIQ1628589.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26421..26861)
                     /locus_tag="AC1ZCC_000302"
     CDS             complement(26421..26861)
                     /locus_tag="AC1ZCC_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIQ1628590.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27167..28132
                     /locus_tag="AC1ZCC_000303"
     CDS             27167..28132
                     /locus_tag="AC1ZCC_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIQ1628591.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28189..28419)
                     /locus_tag="AC1ZCC_000304"
     CDS             complement(28189..28419)
                     /locus_tag="AC1ZCC_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HIQ1628592.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28505..28990)
                     /locus_tag="AC1ZCC_000305"
     CDS             complement(28505..28990)
                     /locus_tag="AC1ZCC_000305"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026183.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIQ1628593.1"
                     /translation="MVGGEEAVYDQVLPLFKEFGTTFTLHGSAGKGQHTKMANQIMIA
                     GTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNYSPRILKEDYTPGFFVKHF
                     IKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDGTQALIKLWWPEGKIPEKK
                     S"
     gene            complement(28973..29391)
                     /locus_tag="AC1ZCC_000306"
                     /pseudo
     CDS             complement(28973..29391)
                     /locus_tag="AC1ZCC_000306"
                     /inference="COORDINATES: protein motif:HMM:NF015411.6"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(29480..30703)
                     /locus_tag="AC1ZCC_000307"
     CDS             complement(29480..30703)
                     /locus_tag="AC1ZCC_000307"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIQ1628594.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30720..31766)
                     /locus_tag="AC1ZCC_000308"
     CDS             complement(30720..31766)
                     /locus_tag="AC1ZCC_000308"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIQ1628595.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(31930..33657)
                     /gene="ptsP"
                     /locus_tag="AC1ZCC_000309"
     CDS             complement(31930..33657)
                     /gene="ptsP"
                     /locus_tag="AC1ZCC_000309"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIQ1628596.1"
                     /translation="MVEMLKGIAASDGIAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(33657..33923)
                     /locus_tag="AC1ZCC_000310"
     CDS             complement(33657..33923)
                     /locus_tag="AC1ZCC_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HIQ1628597.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            34402..36636
                     /locus_tag="AC1ZCC_000311"
     CDS             34402..36636
                     /locus_tag="AC1ZCC_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIQ1628598.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            36784..37104
                     /locus_tag="AC1ZCC_000312"
     CDS             36784..37104
                     /locus_tag="AC1ZCC_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HIQ1628599.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT        10436 a         8034 c         6473 g        12179 t
ORIGIN      
        1 tttgtcacaa ccttttttgc cgagtaaacg gtgttcaaaa gccagtttct cggtatgaag
       61 tcaaatttaa gcaaaaatgt gagaagctat tttcacaaaa tttttcttca tactcgaaac
      121 taagcggctt tttcacgacc tcttttttat tttgccatga acatatcata atacgcgtcc
      181 attatttctt tagcaatcgt caagtttatg tggtcttgtt cgtctaataa gttcggcaag
      241 acaacactga tggcaatttc cggattgtct gttggtccgt atgccacgat attactattg
      301 actgtcgcaa tatctggatg accttccgca accgtttctg cagtccctgt ttttgcggaa
      361 aggtccattt tcgcactggc taatggtttg gctgttgtgt aaggatcggt accatggaca
      421 acttggtgga atccttcacg gaggatcgcc atattttctg cagaaatatt gaccgtattt
      481 tctactgttg tgccaatttc tttcttcact tcacccaagt ttccattttc gtcattgcca
      541 tagatcgctt tgactaggtg cggctgtact cgtttcccgc cattagcgac tgttgaagca
      601 tattgagcta attggattgg tgtatatgtg tcaaactgtc caaatgccaa atcggtaaag
      661 ttacctggag cataccattc tttgccatta tccgcattga acttcttcat ataatcaaca
      721 gaacgtgaga tcccaggaga ttcattcggc aaatcgatcc cagtcgttgt accaagacca
      781 aactctttga aagagtctct caattgctga taagctttag cttcttcatc taaactaggt
      841 agtcccattc caggtgtata gtctagtcct aacatcttta acgccacttt gatcatatac
      901 gtattagaag acaattccga tgctttgact gcatcaagag caacttgtcc gcttcgatta
      961 aagacagagc ttttttctga agcaccttgc agacgaatcg gctcatcaat caaaacttga
     1021 tttccgctga tcgcgttact tgcccagcct gctgtcaaag taccagcctt gactacagac
     1081 cctggatcaa aggcgctagt aatcgtgcct aaagcattct cggtgatttc tttggaacct
     1141 tgttcatgac tgaaaccagt cattgctagc acttctcctg tctgaggatt catagccact
     1201 gcataagcac caggcgaata ctgtgctttg ccgttgttga tcaatgtttg ataattcctt
     1261 ttcaaaattt cttctacttt tgattggaat tctgcattca tagataacat taaattagaa
     1321 cctttttccc cagagaagat ttctttttga ttcgaaacat ttccttcatt atcaagggaa
     1381 acttcatatt gggttttggt tccttgcagg acatcttcat attgtttttc caaataactt
     1441 tgtcctacac ggtcattccg agaataacct tttgctaggt atttctctgc ttcatctgct
     1501 ggaagtcctt gcttttcagt cgtcacactg ccaagaatac ttttcagtga acttgctgca
     1561 ttatattctc gtgtccagtc tgtgccagtc gaaactcctg gaagttcaga agcgcgttct
     1621 gccacaacag ctaactcttg atcagtaaca ccagagtttt tgatatagac agtgtttaat
     1681 tcatatgcac tattcatccg tttaaagatg gtagcaattt ttaattgctc ctcgtttaaa
     1741 ttaatctcat cagctgtgac tttattgacc atcgcactat attgttcgct gttccctaat
     1801 tgcttttctt ttgccgaaag tctttttgtg gcttctttca aatgtttggg atcagccagc
     1861 caaaaatctt ttttatctct atctgtcaga ttttcatcta ccggaacatc gatcagcgag
     1921 ttcagcttcg ttgcgatttc aagcaaatct ttagccgtaa cttgtgttcc tctcgtatat
     1981 gtgatggcct gattcgcttt attcgtaact aaagcttttc ctgtggagtc gtagatcatt
     2041 ccgcgaggag tactttcttg gaccgtaatc gtagaagtag acttcactcg cgcctccatc
     2101 tcttcgccgt tcacgatctg caaataaccc aatctagcaa tcaacgcaac aaataacgta
     2161 aatataatga aaaaaagaaa atttaagcga aaaggaacat gggaccggcg aaacgttttg
     2221 ttcttaggtt cttgtgcacc ttttcttatt ttttctaacc aagaagtatt taatattttt
     2281 ttcataaaat ctttcatcat ttcctatcct tccgcctatt tacattcatg cttcattgta
     2341 cccaaaaact tcttaataaa aaagaaaaaa tgggcggagc aagaaattct ttacctaatt
     2401 tcaagataaa atcaactttt tgtttcacag caatcactta aagttacata tccaactatt
     2461 tttttcatta aaaaataaac ttatttttga aaattatgtt ttcttaagtc gtgaaacact
     2521 ttgatggatc atttatttgt ggaaaactac gttttgttat tctaaaaaac ttatataaaa
     2581 aatagtaagg tttttatttc gaatccataa aatattcttg gattgcttct cgtgtttttt
     2641 tcactttttc gctaagcttt tcgtttttag ccatatagaa ataaaaaatg atatcgatcg
     2701 tagcaaattg ggccaaaagt gaattagttg ccgcacttcg attcgtgatt tcttttggtc
     2761 tagatgtttg tacgagtaca tcggctatct tgctaagcgg gttatttcca aattgcgtca
     2821 acgcaatgat ttcgatattc atgcttttcg ccagctcggc taatgcgact acatctgcac
     2881 ttctacctga gtttgaaaca agccaaagca ctttcttttt ctcattgctg acaagttgcg
     2941 ggagcaagac gtggatatct ttttcaaaaa tgattggctt accgacacga gaccattttt
     3001 gcagaatatc ttctactact aaagaagagg cgccaacccc atataaaaaa acagtctctg
     3061 ccgattccaa acactctacg actttttgga tcgtttcttc ttctagaata tctactgttt
     3121 cttttatcgc tacttgtgca ttgttaccaa gtttgttttt gatagagtaa aaggactcat
     3181 ttgcttctat atcactaaaa ccttcatcga ttccagtctg tagttctgtt gaaatcgtga
     3241 ttttgaagtc cgtcagactt tgaaaaccga tttttttgga aaaacgtaca actgtcggcg
     3301 cactcaaccc ggaagcttct gctaattcat tagctgtcat tgtagggacc ttctcaatat
     3361 taccgataat gtagttggcc aacttttttt ctgctgaaga ataatcttcc atcatacttt
     3421 caatacgact aattacgctc ataagttcct ccaccctttt aatgatagtc tatgtaagta
     3481 taccatatca gacccttatt cacggtcgta tactggataa aaaacaaaaa aacaatccgc
     3541 ccaacagaat atcttatccc atcaatccca cagattctat tgagcatctg tgtctttgag
     3601 gaatacctgt tatctattgt tcggattgct ttctatctac tactttttta caggaaattt
     3661 acctattagt tttctctgct gtaatttttg acttctgctt ccatagcatc aataaagata
     3721 ttcaaatctt ctacagctgt ttctttactt ccataacgac gtacgttcac tgttgcatct
     3781 tccaattcct tgtctccaac aactaattgg taagggatct tttgtgtttg agaggcacgt
     3841 atcttatagc ccattttttc attacgatca tcaacttcta cacgcaaccc cttcatttgc
     3901 aaacgttctt tgatttcata agcataatcg ccatgtgcat caacagaaac agggatgatt
     3961 gttgcttgta taggtgccaa ccatgttgga aaagcgcctt tgtatacttc tgttaaataa
     4021 gcgacaaaac gttccattgt agaaacaatt ccgcggtgga taactactgg acgatgcgta
     4081 ttttccccgt cttctccaac atatgtcaaa tcaaagcgct ctggcaataa gaagtctaat
     4141 tgtatagttg ataatgtttc ttctgtacca agagctgttt tgacttgaac atctagtttt
     4201 ggtccgtaaa aggctgcctc gccttctgct tcaaagtaat ctagttctaa ttcatccata
     4261 gcggctttca gcattgcttg ggctttttcc cacattgcgt catcatcaaa atatttatct
     4321 gtattgtttg gatcgcgata gctcaagcgg aaacgataat ctgtaatatt gaagtcagca
     4381 tatactgcca ccatcaattc aagcgtacgt ttaaattcat ctttgatttg atcaggacga
     4441 acaaatgtat gaccatcatt caacgtcatt tctcgtacac gttgtaaacc tgacaaagca
     4501 cctgattttt catagcgatg catcattcct aattcagcga tacgaattgg taattcacga
     4561 taagaatgga tcgtgttttt gtagaccatc atgtgatgag ggcagttcat tgggcgaaga
     4621 acaagcattt cgccatctcc catatccatt ggcgggaaca tgtcttcttg ataatgatcc
     4681 cagtgtccag atgttttgta caactcaaca tctcccatta ttggtgtata tacatgttgg
     4741 taacctagac taacttcttt gtctacgata taacgttcga tcgtgcgtcg gattgttgcc
     4801 ccttttggaa gccagaatgg caaaccagaa cctacttctg gggaaaccat gaacaagtca
     4861 agttcttttc ctagtttacg atggtctctt tcttttgctt cttcacggag acggatatat
     4921 tctttcaaag ctttcttgtc aaaaaaggct gtcccgtata cacgttgcat catttgattg
     4981 tttgagtttc cacgccaata agcacctgcc actgataata atttaaatac ttgtatacgg
     5041 ccagtggatg gaacatgtgg tccacgacac aaatcgatga attcgccttg ttgataagca
     5101 gtaatcactt cttcttctgg caattcacta atcagttcta ctttgtacgg atcacttgca
     5161 aaaatttcta atgcttcctg ttttgaaaga acacggcgtt cgatcggcag attttctttg
     5221 acgattttca tcatttctgc ttcgattgcc ggcaaatctt ctgcagttac ttggttttgt
     5281 ccattatctg aatcgtagta aaaccctgaa tcgatagctg gtccaacgcc aaagtggata
     5341 tttggataga ggcgacgcat ggcttgggcc atcaaatgag ctgctgaatg acggactaag
     5401 ccaagtgcat cttcatggtc tggtgtgatg atctcaatac ttgcatcttc ctcgattgga
     5461 cgagtcaaat caacgagttt tccgttcact ttaccagcta aagctttttt tgctaagctt
     5521 tttgaaatgc tttcagcaat tgccaatgtt gttgttccag actcaaattc tttgactgca
     5581 ccatctggaa aagtaatttt tatcataata gtttcctcct tgaaatgttt tgattgatca
     5641 gatcatcaag ctgaaaaaca aaaaaatcct agcatctctt gaaaagagat actaggacga
     5701 aaagttcgtg gttccaccta atgttcaaac gcaaatgcgt tcctcaagcg ttgtaacggt
     5761 ccgaccgccc ttgcttcaac aagggtttcg aaagtggtca tcctttggtt ttctcctgga
     5821 aaagtttcag cctagctttt cctctcttat cgaagactct caaattcagt tggctttctc
     5881 atcaatcaat tatgtgttta tttaaccact aataaataga aaaatcaagt gggaaactaa
     5941 acttattttt tccattcccc atttacaccg tatgttcctt ttttcatctc attgatgatg
     6001 acatggatgt gttcttttgg tgcctgcgtg tttttatgaa cagcttccgt gatatctttc
     6061 atcatatttt ctaattgttc cggactgcga ccttctacta attcgacatg tacgaatggc
     6121 ataatgctct ctcctctttc tgatcgttct tttttcaact cgattctatc atactatgtt
     6181 tgtcttgggt actgaatcag gaaaaatggc tggatctttt tttgctctca aggcactcaa
     6241 aatagagacc gtatccacga cttcttgcaa aacagctccg aataatgcgg ggattagtcc
     6301 cgtacttgca attaacatca aaaaaataca aataaagatg ccaatcagta cagactgttt
     6361 cgctattctc atcgtatctc ttgaaatcac tactgctttt gctacacgca agagatcatc
     6421 tttcaagatg accgcatctg cactttcact agcagctgta gaaccatgag cccccattgc
     6481 aataccgata tctgcagcag caagtgctgg tgcgtcattc acgccgtctc ctaccatcgc
     6541 aacaggtctt tgatctttag ggatactttc taaaatggtc agtttatctt gaggcaagct
     6601 acgggcatgt acagaatcta attggacttt ttctgcaatc gtttgagcaa tggcagatcc
     6661 atccccggtg atcatcatca aatttttcac atcaagtttt cttagctcct tgattgtctt
     6721 tgctgcttct gggcgaatct gatccgaaaa taggatcgtt cctgcaaaag atcgatcaat
     6781 tgttacatag attgctgtat tggacgtttc tatttcttca gctgaaacaa aagaggcacg
     6841 tccaatcttt acttcatgat tattgactac tgcttggatt ccttgaccgg tcacttcttt
     6901 cagttgacta acaggataca gttggctgtt tttcacgttg ttcactaacg aacgagctaa
     6961 gatatggcta gattcctgtt ctgcactagc tgcatacaga agtagctctt ctttttttat
     7021 tccattgact ggctgaatcg cctctacttc caatatccct ttggtaagcg ttcctgtttt
     7081 atcaaaagca atcgtttttg tacgtgcgag cttttcaatc gttgttccag tttttacaac
     7141 gatcccgttt ttacttgaac ggctcattcc tgcaactaaa gcaatgggcg cagctagaat
     7201 cagtggacaa ggtgatgcca ctaccagtac ctcagcaaat cgtaaagggt cttttgagaa
     7261 ccaccaagaa ataccaccga tcaaataagc aatcaacgtg aaagggactg cataacgatc
     7321 agctaaacgg acaaatcggg caggcttttc ttttgattct tcgactagtt taataatcat
     7381 ttgatactgg ctgtctgcgg cacgtttttc agcgatcatt tggatcgatc cgtctccatt
     7441 taaagaccct gacataatct gatctccagg tttcttggtt tccgggcggg actcacctgt
     7501 caaagaagat tcatcgaccg aagattctcc tttggtcact cgaccatctg cgggtacaag
     7561 ttcgccaggc ttgactagta actggtcatc aatatttatt tcatcgatta ctatatcctg
     7621 caattttcct tctttgaatc gatgggcaat tcttggggtg ttatctaaca aagactgcag
     7681 ttctttaccc gctcgtttat tcgcgtaatc ctctagacta tctccgcccg taagcatgat
     7741 caatatcatc aaactagccc agtattcttt gacggctaat gttgcaagga ttgcggtaat
     7801 cgctaataca tctacgccat atcttcctga acgtaacgtc ttgatcattt ccaaaaacat
     7861 caagaaggcg agaatccctc cagtaaatgc gacaatcgca aatgcaaggg ccggctggtg
     7921 aaaaacaaat tcacttaata aagctatccc accaataagc aaagtaagta aaaatttttt
     7981 gaaatgtgtc attgtttttt ctcctctttt atgtgactta gagaggttag taataaattt
     8041 ccagctgcct ctttcatctt aagcatagtc cttgactagc actttttaaa acaataggcc
     8101 ttaattgaaa acaactatca ctatcagcgt ttccaaaatt catcagttct acaaacaaaa
     8161 atattcagca aggattactt tttcgattca tcagttaaaa agtacctttt ttcgaaaaac
     8221 tatgctatac ttttaattcg ttatttacgg cggaaggagc aaacatgcga tgaatgagaa
     8281 acaactagaa caaacgcacg tggatgaaac gatcgaacta atccagatcg aacaaaatat
     8341 gctggataca aaacaacaag aattgactca ggaaatgaat cattcgacaa aagacacagc
     8401 taatcacaag attcgagctg gttccaatga agctttttat gaatctgctg ttgaatatcg
     8461 ccaacatgag caggaattgc ttttgaaata ccatactctt gaagcacagc aaaaacgcct
     8521 gcaaacgttg tctgtcatga aagatagtcc ttattttgcc cgtatcgact ttaaagaaga
     8581 ccgtgaaaca gaaacattat atctaggtat cgcttcttta agagatctgg cagaagaaac
     8641 aatcgttatt gactggcgcg caccaattgc caatctttat tatgaaggtg agattggtcc
     8701 cgcattttat gaaactgaaa cagatagaat agatgtcgat ctgcagttga aacgacaatt
     8761 taaaatccaa gaaggacgta ttttatctat ggtagatacg tcggaagtaa tcaatgatga
     8821 ctttttgcta gagatattgg atgaatcatc aagcaaccat atgaaaaaca tcgtctctac
     8881 gatacaaaaa gcacaaaatg caatcattcg ggacacaaca agtaaagtaa tgctgattga
     8941 aggaatcgcc ggaagtggaa agacatccgc tttgcttcaa cgtattgctt ttctgttgta
     9001 tcataatcgc aagtggctcg aggctgaaaa cgttcttcta ttttcaccca accatttgtt
     9061 ttctgattac atctcgactg tattgccttc attaggtgaa agcggcgtgc ctacacagac
     9121 attcaaaaat tatttggtgc agctgctgcc agagttttct ttactagatg aacagcaaca
     9181 agaaagtggt tttttgctag gggaaaaaga tccgatacaa gtgatgaaaa gcggattgac
     9241 cttagtcgat cagattgatc gctatatcca aggcatcaca aactacggtc ccttgttccg
     9301 tgatatgaaa ataaatggcc gtacgatcct atcaaaggaa tcgattcgcc aatggtataa
     9361 agaaacaaat gaacaattac ctttacatca tagactttct ctattacaaa cgaagctcct
     9421 gaaaaaatta ggcggtctgc aaaaagacga aacacgtcag caatgggtca aagatctggc
     9481 ggaagaacag ctccaagaac tatacgcaac agatccaaat cttgaatata cagaacaaaa
     9541 ggagcgttca ttacgtaaaa aactggccaa ccaaatcgta cgtaaaagat tccgtcgaat
     9601 ccatcgaggg atcatgaatt atcaatttgt caatataacg aaacaatatc tccactttct
     9661 acaaactgtg ccactttctc ttctaacacg acataaaatc agcgagacca ggtggcagga
     9721 acatttgaca actgttcgta cacacttgcg tcaaagagaa ttgcgtcaag aggatgctgt
     9781 actattcttt ttactgatgc gccgaacata tccagtgacg gttacacaca aagctcgcta
     9841 tatctttatt gatgaaatgc aggatttcgc accatcacaa gtggcattgc tgcaagcctt
     9901 gttcccaaaa gcaaatttga ctttctgtgg agatctaaat caaaatgtct ttggaaatga
     9961 aacgatcgtt ggatcattgg aagacctttt cgtggaccgt gaagtcactc gtttccaatt
    10021 aacgacaagc tatcgctcca caaaagaaat cactgatttt gctaatcatt ttattgtgaa
    10081 agaacatcaa gtagaaacaa cagcaagaaa aggagacctt cctttgctca tccaaggaac
    10141 aacagatgat atcaaattga attggttagc agataccatt cgaacgactc agggaaaagc
    10201 ccggtattgg cgtacagcaa tcatagctaa aacacaagaa gaatgcgaag ctctttatgc
    10261 tcaactgcca gacgagttga aacaacaagt ccagctgatt ttaaatgaga gtgactttat
    10321 gaagcgttcc attattttac ttccagcctt tttagcaaaa ggtctggagt ttgaccgagt
    10381 tttcttgtgg gatgtagatg cagaacattt ttcttctcag caagacaaat tgatccttta
    10441 caccatgtgt acacgtgcga tgcatgaatt gacccttttt acctccaata atgaaagtcc
    10501 tttgttaaac aatatggata tttcatctta tgagaaaaaa gtactgtaag taaaaagaca
    10561 actgctatca ttaatgttag cagttgtctt tttatttaca ataataattg cagaaaaagt
    10621 aattcaatca tttaaggtca tttattctat atataaatcc aaatctgttt caggatattt
    10681 taatttgaca agcgaaataa tcattttcag ttcttccatg ttttctggat attctgggtc
    10741 aaataccttg ctatgacggc caatcccgcc attgtcgatc aatcggtaaa ttcgattcca
    10801 ttcaatgaat tggtcaattg attggaaatt gctgattgga tagccgccac aatccaaaat
    10861 ctctgataca cgaaatgcta ggtatgcact atcgatcact ttattttttt tcgtgtctat
    10921 cttctcattt ctttcgataa gaattttttc aggacgatgt ttcaagctcc actctaatag
    10981 tagaaagaaa gcctccatcc gatactcttc ttcaaataac cgaaaggctg aatcagaacg
    11041 acgaaaaagc agcggaatca gataattttg tctataaatc agcattggtg cgcggtccac
    11101 catacactct aaataacggt tcagataccc tgtaccgaca tagtttccta atcgatagcc
    11161 ttcaacgccg aatcttgcgt atttttgcgc taattttgaa caatcagtta cgttcacagc
    11221 taactcttct actttgcggt aaccgcttaa gatacgttca aaaggttcta aagtgatttt
    11281 tgcttccata ttcttccccg tccatcttcc acatacttca tatcatttca catgatactt
    11341 atgctctcat cataccacag gttgaattag aaaagaaaat aaatcacaca aaaaagcaag
    11401 acaatctgtt agtgagattg tcttgctttt cgaaaaagac tgaatacttt tcgttaaaac
    11461 tggttgctta gtcttctaat gccacattat ggaacacatg ctgtacatct tctagatctt
    11521 ccagtgcatc aatcattttt tcaaatgttt cttgatcttc tggagatagt gttacctcgt
    11581 tttgagggat catttggatc tcagcgattg aaaaatcagt gattcctttt tcttttaatg
    11641 cttcttgtat actatggaaa tccgctactt caccgtaaac gatgatttgt ccttcttctt
    11701 ccattacatc gcgaacatcg atgtcttttt ccattaggta ttcaaggatc tcgtcagcgt
    11761 cttctcctgc aaaagcaaag attgctgtat tgtcaaacat gtaagaaacc gctccggaaa
    11821 cacccatgtt cccgccattt ttcccaaatg ctgcccgtac gtctgctgct gttcgattca
    11881 cattgttcgt caatgtatca acaatcacca ttgagccgct tggtccaaat ccttcatagc
    11941 gcaattcaga gtatgtttca tctcctgcac cttttgcttt ttcgattgct cggtcaataa
    12001 tatgttttgg tacattataa gttttagcgc gatcaatgac gaaacgtagt ttttggtttg
    12061 catgcggatc aggatcacct gattttgctg ccgcgtagat ttcaatccca aattttgcat
    12121 aaatccggct attattagca tctttagcag ttttctttgc tacgatattt gcccatttac
    12181 gtcccatatt ttcacttcgc tttctgattt gatagtttca taacgcaaaa acgtcctgtt
    12241 ccattatact tgtgaatcac gattctgtga agcttttttt ctttcttcct tcattcttct
    12301 catttttagt caattatcta tactcggatt gggatttgcc cgaaattctg ttccggaata
    12361 ttgcctccca attgatagat ttgatcactt ctaactaaca gttccgcttc ttgtgcctct
    12421 ttttttccga ttcgggaata aatagaaatc tctttttttt cttttaagaa tcttcttcca
    12481 gcatcggtaa atccaagtag tcgaagataa tttttatttt gttggacagg aatctcttgc
    12541 tttttgatat tcagcaagac ataacaagat aatcgttgga tccgtgtcca tgtatatctt
    12601 tttgttttta cctttgacac aaaatcctca aaactatcag cagttgcagc tgctgttttc
    12661 atgcgcgatt ctattccttc ggtcatctga tagatatctg tcagttccag caaagtagaa
    12721 gataacaacc gataacgcaa atacggccaa taagattccc aagtctgcag cgaacaagtt
    12781 gaaagatctt cttctgtctg aggcggcaca agtccagcta ttctttttcc ttcttctaac
    12841 gcatggcgaa tggctgttgc actggctatc ctctgatctg tgatgatttt ttcatgatag
    12901 cctgcacttt tacgtcggat cggatgtaat tccatagggt ggacatactt ggcattttcc
    12961 tttgcataac ttaatcccaa gatatgattt ggcgaagaaa aatcaagcgt aatatcggga
    13021 tacagactgc gaaaaacctc agtcattttt tgcggataac tatacgtttg atcagacagt
    13081 tcttgaaatg cctcttctat cctatcttga ttctcttgga caaagcgtcc gaaactgctg
    13141 taatcaaacg aagtctcttc ttctgttcca aaacacaaac tgtcacagtc cagtgtttgc
    13201 aagattttta ctgcaccttt agcaaaatag tcagctgact gaaccgccca ttcgacggga
    13261 agctccacta ctaaatccac accattttgt aaagcttcat tcgcgcgatg ccatttatcg
    13321 atgatagccg gttcgcctct ttgcaaaaaa ttcccgctca ttacagcaat gacgacctct
    13381 gctcctgtta tttttcgtgc catttccaca tgatagcgat gtccattgtg aaaaggattg
    13441 tactctacga taatcccgca gcttttcatg cgcatcctcc ttcatagtta tcgataaaaa
    13501 agaaatagag acaagtaaaa aaaggcaaaa atcggaaaaa atacccgaac tatataaact
    13561 attctcgcaa acacaatact tctgcgacga aaagctgcac tgcaagagca atgcctatat
    13621 cgttcggata tataatttat gttgaagatt tattcttttc ttgttaactc gagcaggcaa
    13681 aaccgctagt ctttgtatgc tacaaagaac cagcgctttg atttctctga tgctgcttcg
    13741 tctgtaaaat cagaaaatgc ttcgactttc gaaaagccgc tgttttccag cattcttaaa
    13801 taactttcca atggataggt acgctcttga tgtagttcat cttcacgaat aaatcgacca
    13861 gacccttctt gttcttcaac gaaaaaggta agaaaatgtt cgatgctgtg tttctcgctt
    13921 cctggataac tatcccacaa aaaagcaaaa ttatcccctt gataatgata gctatattct
    13981 ggaaaaacct catctacttg ataggtggag tgtacgtcaa ataaaaatcg gccattttct
    14041 tctaacgctt gatagacttc atcaaacact tgctgtacct cttgtctatt tttcatatag
    14101 cacagcgaat cagaaaaaca ggtaattgcc tgatattgtc cgatttctga taaatcgagc
    14161 atattgcctt ccacaaattg gatcgatgcg tcttcttcat atgctcgctg gctggcaatc
    14221 atcaacatct cttctgaaag gtctaaccct gtcactttat atccatcttt tgcaaaggca
    14281 accgctaatg ctccagtacc gcatgccagt tccaaaatat tttttgtgtc ttcttgtaaa
    14341 tgacgtttcg aaaaagctaa ccattgctga taaagtgttt cgtccatcac ttcatcgtaa
    14401 atgaaagcaa atgtttcata agccatatgt taatccaacc attcactaag atcgactagc
    14461 ggagcatctg accatagttt ttccagatta tagaaatttc gttctggcgc atggaaaaca
    14521 tgtacgatca catctccaag atcgatcaat acccattttc cgccttcttt tccttcaata
    14581 cgttttactt catatttgtt ttcttcttct ttatcaatga tttcttctgt aattgcgttg
    14641 atttgacgtt cgctatttgc tgaacagatc atgaaataat ctgccaacaa ggaaacattt
    14701 cgaacgtcta aagcgataat gtcttctgcc cgttttgaat ctgctgcttc aatcgcaatt
    14761 tttaattgtt ctttactatc tatgatgatt tcctcctaat atgagcagct tatttttttg
    14821 ctacccagta attatatgtt tcgattgttt ttggataaat ctgttgttct tgcgcaagca
    14881 aatgggccaa tgtatgtttt gtttcaaatg ctacagcttc gtctagatca gcccatgcaa
    14941 tggctcgtgc atcttgtacc ccaggaaaat ctcgacctgg ttcaatatag tctgctacat
    15001 aaatgatttt gtcaaggaga ctcatcttag cagccccagt cgtatgcacc cggattgcat
    15061 gaagaatctc tgcatcggtg attcctaatt cccgctctac aaacgaagcg ccaacaagac
    15121 catgccagat tgcattatta taattcaata actccggatc aaaaccgtct cttacgatta
    15181 ccattttgaa ctcctcatct ggtcgttctt ttgcataatc atgtgtcaat gcagcaatac
    15241 tagctttttc aggagaagca ccgtattttt tggcaagagc cacggctgtt tcttctactc
    15301 caagcacatg cttgaagcgt tgttcgctca ttctcatctg tattttttgc atcaggtttt
    15361 ctctagaata agcagtatat tcattactat agtccatatt catacaaccc ttcattttga
    15421 atataatcaa ttactttatc tggaacaagg tagcggatag aacagccttc tttgatcttt
    15481 tgacgtattt tcgtggaact gatgtcgatc tcaggtacat ctacccaaat tacaggatat
    15541 ggagtatccg ttgtgtatcc tggtcgacgt atacccacaa agttgaccat agaagtcagc
    15601 tcatcgattt tataccattt tggcaaatat tctaccatat ccccaccgat aataaagtaa
    15661 taatctgtat ccggattgtt ttgcgtcaat tccttcatag tatcataggt gtaacttttc
    15721 ccaccacgag ctaattcaat cgtttcaatc tgcaaaaaag gattgtcttc caccgctaat
    15781 tccaacatgt tgagtcgatg ttttgcatca atcgtctgtt tctcatcaac atgaggtggc
    15841 tgataagaag gcatcaaaaa tacgcggtca agacctaaat tccggccagc ttgttcagcc
    15901 atcactaaat gtgcaaggtg aacgggattg aatgttccgc ccaagatccc tacttgcttt
    15961 ttcttctcta agaattgagg catttcttca ggaaataatt gtgaacgaac aaatgtctta
    16021 gcttgcgtat tcatcatggt cactccttat attgctttta cttcttttga tagcctttga
    16081 tacttttctt ttgtggaagg tttgtataat acaataacac gtccaatgat ttggacgatt
    16141 tcgcaatcta tttctttttc taatacttga gctgcttctt ctgcaatctc atctgtattt
    16201 tgaagtaacg atactttgat cagttctctt ttttctaaag cttcttcaat ttgaacgatc
    16261 attgctgaat tgatcccacc ttttccgatt tgaaaaatcg gctgaagatg atgcgctttg
    16321 ctacgtaaaa aacgcttttg ttttcctctt aaactcataa ttttccttct tccttaaatc
    16381 aatgctttgc gtttcaatac atctacaccc tttggcgccc agcccgcaat tactactggc
    16441 gctgttacag tgatccagcc tagaccggca aaaacgatgt ctgttttttc tttcacagaa
    16501 aactcaaaac gaacaagctc cggaaatgcc tcgacttcat caggacgagg aggctgtaat
    16561 aatccaccta catgtttttg ataaaattcg tctgcttttt ccagtttcgt tcgatgaata
    16621 tttacatcat ttgacacata agcaataaat gaagcacgat tgccgctgat aaaatcaaat
    16681 cttgcaagtc cgcccaagaa aagtgtttgc ccttcattca gctgataaac tttaggtttg
    16741 atttctttta acggtgcaat gatccgcaaa tcttttttac ctaagtagtg cgccatttga
    16801 tggcgatgga taattcccgg tgtatcaatc aaaaaatgcc cgtcatctaa aggaatctcg
    16861 attttatcca aggtcgttcc tggaaactgc gaagttgtga ttacatcttg tactcccgct
    16921 gtatttttga tgatttgatt gataagcgtc gattttccga catttgttac tccgacgata
    16981 tagacatccc gtccatcgcg gtattcttcg attttttcta atagttccgt catctcatga
    17041 gatttcttag cactagttaa taatacatct actggacgaa ttcctgcttc gtaagcacgt
    17101 tccttcatcc attgtgttaa tttagttttt ttcaatgatt taggtaatat atccactttg
    17161 ttcccaacta aaagtactgg attgtcaccg acaaaacggt gtagtcctgg gattagtgaa
    17221 ccattgaagt caaaaatatc caccacattg acgatcaatg catctttttg ccctaattca
    17281 ttcagtaagc gcaaaaagtc gtcatccgtc aatgagacat cttgggtctc attataatgg
    17341 cgtaatctga aacaacgctg acaatagact tccccggaag ctaaaccttt ttccaaagcc
    17401 gtttgcggcg tgtatcctaa ttcaccgggt ttgtttgttt gtataacggc accacagccg
    17461 atacattgta attcttctgt cattcgattc cacctcgcca gcccatatca gggtgttttg
    17521 ctaatagata ggccataatt cttttttccc aaaaacgatt aaattgtgtc ttccaactat
    17581 ctgttgcgat aatgggtttt acaaggatgc tacgtatccc tgcacgattg gctccacgta
    17641 tatcagtcat aatctgatcg cctaccatta ctatctcatc atctgctaaa tccaattgtt
    17701 tctttgctat gttgatccct cttgctaaag gtttcattgc tcgtgcaaca tacaaaagat
    17761 caaatttttc aatcgctcgc gcgacacgtt ttgaattatt gtttgaaaca acaacaacag
    17821 ggattcctgc atttttcatt tctaaaatcc aagtaagcaa ctcttctgtt ccatctggat
    17881 tattccatgc aatcaacgta ttgtctaaat cggtcaatac tgctttgatg cctagattct
    17941 ttaattgggc aggtgtaatc tgatagatgg cttctaccat ccatgttggt ttgtaatttg
    18001 aaaacatgct gtctcctcat ctgcaaaaaa tgaaggagca cataaagtac tcctcaaccc
    18061 cgtattatac tgcatatcag caaaaaaaac tacctctata ctcgttcgtc tttcagaaaa
    18121 agcatttctt tacaaaatga ccaaaaataa agattgatca cagaaaaatt tagaaacaaa
    18181 aaacaactaa tattggcgga acctcttgta tcgtttttcc agtaatttat gctgatctaa
    18241 tcgaacaagt tccgtcaatc tttcaatcaa cgcttttttc ataatgtgtg cgactttata
    18301 tcgtggcaac tgctcgccgt tgaacgtttc aggaataact cgatcaacga ttcctaactc
    18361 gcgtagttct ggcgcagtaa ttttcatcaa ctcagctgct tctgcggcgc gactaccgtc
    18421 tttccacaaa atcgacgcaa atccttctgg agaaagcaca gcataaatcg tttcttccag
    18481 catccagact tcatccgcta aacctagagc aagggcaccg ccacttccac cttcaccaat
    18541 gatgatggcg atgatcggta cctttaaatc agacatttcg agcagatttc tggcaatagc
    18601 ctctccttcg ccgcgctctt ctgctcctac accgcaatat gcacctgctg tattgatcaa
    18661 ggtaatcacc ggtctattga acttttccgc ttgtttcatc agtcgtaaag cttttcgata
    18721 gccttcagga tggggagaac caaaatttct gcggatattt tcctgcagat cccgtccttt
    18781 ttgaataccg atcactgtta ccggtttgcc atctaatcga gcgattccac caacaatcgc
    18841 ttcgtcatct gcaaataaac gatcaccatg gcattcctcg aaagaatcga agatctcttc
    18901 aaaaaaatcc aagcttgtcc atcgttcttt agctcttgct aactgaacga catcatgtgc
    18961 tgtcttatcc acgatctatc catcctttct gtgtatgcaa ttcaagaagc gtatggattt
    19021 tctgtttcaa ttccatgcgg ggaacaatct gatcgacaaa accatgagat aataagaatt
    19081 cagccttttg gaaatcttct ggaagttcct gcttgatcgt ctgttcgatg acgcgtcttc
    19141 cagcaaaacc gatcaagctt tggggttctg ctagaataat atcgccttcc attgcaaaac
    19201 tggcagttac tcctccggtc gtcgggtccg ttaaaacagt tagataaaaa agaccttcat
    19261 tgctatgccg tttgacagca gcagagattt ttgccatctg cattagagaa aagattcctt
    19321 cttgcatccg tgcgccacct gaagctgtga acaagacgac tggaagactt tcctttgttg
    19381 ctcgttcgaa taatctggtg atcttttctc cgacaatcgt tcccatactt cccatgataa
    19441 aattaggatc catcactcca ataatgaagg gaatcccctg aatggttgct ttaccagtga
    19501 ggactgcttc atgaagtcct gttttatctt gcattttttt aattttttcc gtatattcgg
    19561 gaaatgctag cggatctttg gttacaagat ccgtatccca ttcttcaaaa cttttttcat
    19621 caataatcaa agctagacgc tgccatgcac cgattcgaaa agcgtatccg caatgagggc
    19681 atactttttc ttcaccgatg tctttcgtat aaaggatatg tttgcaattt ggacactttg
    19741 cccacatgtt atcaggaaca gatggcgagt tagcaggttt gctctctcta ttaggattga
    19801 tccgaatata tttctttttc ttaaacagag ccatctatgc gcctcctatc tccgtttctg
    19861 gtgtccaatt cggtaaaaat tcttcttgta aaaatgctgt gctgtaatca cctgctatga
    19921 ctgcaggatg actaatcaaa tccatctgaa attctgcgtt cgtgatgatt ccttctgtga
    19981 taaattcact taaggctcgc tgcatcttca ttaatgcttc aaaacgcgta tttccatgga
    20041 cgattatttt tgcaatcatt gaatcataat atggcgggat cgtatatccc gggtaaactg
    20101 cactgtctac tcgcagtccc atcccacctg ctggaagcag taagttttgg atcttccctg
    20161 gagaaggagc aaaatggaaa gcaggatttt ctgcattaat ccggcattcg attgcgtgtc
    20221 cttggaatac tacttcttct tgacacacat tcaaaggctc accggctgct atctccacct
    20281 gtttttttac taaatcgatt cctgtgacca tttctgttac tggatgttcc acttggattc
    20341 gagtattcat ttccatgaaa taaaaatcgc cttcttcatc catcagaaac tcgatcgttc
    20401 cggcattttc ataattgact gctttagctg ctcgaacagc agcgtctcct agtgcttctc
    20461 tttttgtttg tgagatcact acagaaggag attcttccag tactttttga ttgttgcgtt
    20521 gtaaagaaca atctcgctcg cctaaatgaa tgacatgacc aaatcgatca cctaagatct
    20581 gaacttctat atggcgagca ggataaatga ttttttccaa atacatatca tcatttccaa
    20641 atgccgctgc tgcttcttgc tgagctgaag aaaaatgctg tgggagctct tcttttgatt
    20701 gaactttccg aatgcctttc ccgccaccac ctgcagctgc tttcagcatc actggataac
    20761 caattctgtc tgctatttcc aatgcttctt ctactgtaga taatacacca gtacttcctg
    20821 gaatgactgg gacatttgct tcttgcatca attgacgcgc attgatctta tttcccattg
    20881 catcgatcgt tgcactttta ggaccaataa atgtgatatt gcactcttca cacatggagg
    20941 caaatcggct attttcagat aaaaaaccaa atcctggatg aattgcttcg gcttttgtca
    21001 cgattgcagc gctcagtact tcctgaacat tcagatatga atcaacggct tttgccgggc
    21061 caatgcagat tgcttcatct gctaactgtg tatgaagtgc ttcggcatct gcttctgaat
    21121 atatcgctac ggtttggatc cctagttcgc ggcatgcacg aatgatacgt acagcgattt
    21181 caccgcggtt agctatcaat actttagaaa acatatttca cctatccaat cataaaggtt
    21241 aattccgctt cggctacttt tttaccgtct actttagcga ttcctttgcc tattccggct
    21301 gaagctttca ctttcaatat ctcaacttca aggtataagg tatctcccgg tgtaactttc
    21361 ttacggaatt ttgctttgtc caatccgccg aaataagccg tctttccctt gaattgttca
    21421 agagaaagta atgcaactgc tcctgcttga gccattgctt caacaatcaa tacccctggc
    21481 ataacaggtt cttctggaaa atgtccttga aaaaagggct cattgatcgt tacatttttc
    21541 ttagcaacga tccgttctcc ttcaaccatt tcttccactc gatcaatcaa aagcattgga
    21601 taacgatgtg gaatgatttc ttttatttct tgtatgttca ttgttttccc tctttcctgc
    21661 tttgcttatt tttgctgaat cctaaagagt ggctgtccat attcgacaac tgactcgttt
    21721 tctacaagga tttctgtgat cactccgtcg gcctcactag ttatctcatt catcagtttc
    21781 atcgcttcaa taatgcagat aacttctcct ttggcaactt gatcacctac ttttttgaaa
    21841 gctggtttgt cgggcgctgg ttgcagataa acgattccca cgattggcga aacaacctcg
    21901 attccatcaa atactgccga ttctttttct ttcattattt ctggagcagt tgttgcttgt
    21961 tcaggtatga cattgactgg ctccgaaact ggcaacgttt cgtttgtttg aaccatattc
    22021 atgcctcgac ttgttttatt tttattcata tacaattcaa attgaccttc acgcaaatcg
    22081 aattccgtta gcgaggattg atcaaattga ctgactaatt ctctgatttc attgatatcc
    22141 aaaattattc ctcccagcgt ttcatgcaca gaaccgcatt atgtccgcca aaccctaatg
    22201 aattactcat tgcataagta aaacttgcag gtttgctttc gtttttgaca actgtcaagt
    22261 caatctcagg atcttgtttt tctacattga tcgtaggtgg gataaattga tgttctaatg
    22321 ccagcaaagt tgccaaggct tcgatcccgc cggctgctcc caataaatgt ccagtcatcg
    22381 atttcgtgct gctgacgtaa gtatctctga aattttcacc taatgcgatt tggattgctt
    22441 ttgcttctgc ctggtcattg gctggtgtac ttgttccgtg agcattgata tatccgactt
    22501 gttccggctg tacaccagct tcttccatcg caagcttcat tgctttagat gcaccagaac
    22561 catctggagt aggagctgtc atatggtaag cgtcacagtt tgcaccgtat ccaacgattt
    22621 ctccatagat tttggcaccg cgagcttgtg catgttctaa agattcaaga actagaacgc
    22681 ctgctccttc acccatcaca aaaccattcc gattttcgtc aaacggaatc gaaccacgat
    22741 taggatcagt tgattctgag agtgctgtta aagctgcaaa tccagcaatc cccatctcac
    22801 aaatcgttgc ttctgttcct ccagcaagaa tgacatcaga atagccatgt ttgatgttac
    22861 ggaatgcttc cccaattgaa ttcgttccgc tagcacaagc tgttacgata gctgtacaaa
    22921 taccttttgc acctacacgc aaagcaatat ttccagctgc catgttggca atcgccattg
    22981 gtacgaacat tggagaaact cgttttgcac ctttttcatg tagacgaata acttggtttt
    23041 cgattgttgg aagaccaccg atcccagatc cgatgatgac gccaaaccgg tcttgattca
    23101 ttttttcttt atctaaaccg ctcatctcca ttgcttctaa agcagcgtag attccataga
    23161 tcgaaaacat atccattcgt ttgctatctt ttttaataaa atatttatcg taagggaaat
    23221 ctttgacttc acccgctaag gtaatccctg tttctgaagc atcaaattta gtgatcggtg
    23281 cgatgccatt cgtcccctct tttaagctat tcaaaaagct gtctgcgtca tttccaatag
    23341 gtgaagtgac tccatagcca gtaattacca ctcgattcat ttgtttgctc tccttttctt
    23401 atccgtgcat gaccaatccg ccatcgacat tgatgacttg tccggtgata tatggatttt
    23461 gtgctaagaa aactgctgtt tgtgcaatat cttctacttg tccgaaacgc tgcatcggaa
    23521 tttgcttttc cgcttgttcc ttgactttgt ctgctaaaac ttccgtcata tcagttgtga
    23581 tgaatcctgg cgcaatggca ttacatgtaa tgcctctagt tgcagcttct cgtgccacag
    23641 attttgttaa tcctactacg ccagctttac tagctgcata atttgcttga ccaatatttc
    23701 caatcaaccc agatacacta gagagattaa tgatggcccc ttctcgctgc ttcatcattt
    23761 ttttcaatac gtgctgcgtc atattgaagg ttccaatcaa attgatatcc agacattttt
    23821 tgaaatcttc tgcatccatt cgcatcacaa gtttgtcatt tgtgattccg gcattgttta
    23881 ctaacacatg gatcgatcca agcttttctt ctgcttcttt gatcatttgt cccgctttct
    23941 cataatcaga gatgtcaccg gaaactccta cgcattttac accgaatgct tcgatttctt
    24001 ctatcttttc ttttggaatc tctccgcgac cattcaaaat gatatttgct ccagcttttg
    24061 caaaagctaa ggccatcgct ttgccgattc ctcttgtact acctgtgata aatacatttt
    24121 ttccttttac atccataacg ttcctcctta tagtattgct attgtttcat caaatgtctg
    24181 cttattctca acacgcagaa ctggtattgt tttatcgatt tttttcataa atccgcttaa
    24241 tacttttccg gggccgactt cgatgacttg ttcgatgccg attgttttta acttatggat
    24301 actctcatag aaacgaacag gttgcatgac ttgctgttca agtagtcccg cgattgtttc
    24361 tttcttcatt atttccgttg ttgtgttgct tatgatagga aaagaaggtt cagagaattg
    24421 aatctgactt aggtccttcg ccagtttttt tgctgccggt tctaaaatag ctgtatgaaa
    24481 aggcccgctg acatttagag ggatcattct tttgaaccct ttttctttta atagtaaaac
    24541 agcttcgtct actgcttttt cttcgccacc aatcacgatc tgctgaggtg tattataatt
    24601 tgcaggagat acgatgccgt acttgctagc ttcatgacag ctttcctcga tcgtttcaat
    24661 cggcgcattc atcactgcaa ccattttccc gcttccagct ggggcagctt ctgtcatata
    24721 agcgcctctt tttgctacta acgcaactgc ttcagtgaag gatagcgcat tactagctac
    24781 taatgccgaa tattctccta aactcagtcc taaagctgca tctgggatga ttccatgctc
    24841 cttcaataag cgataatacg cgatgctgac tgttagaata gctggctgtg tatattgcgt
    24901 ctgatccaaa cgttcatttt ctgtaaagca aagttcagcc atatcgtagc cgagaatctc
    24961 acttgcttca tcaaatgtct gctttacaac ggcttcttca tataaatctt ttcccatacc
    25021 ttgatattgt gcaccttggc cactaaataa gaatgctgtt ttcatgtttt tctccaatat
    25081 atccagaata tatgtttgat tcttcctatt caaaaaggcc gagacattcc tatttcagga
    25141 aatgtcccag cccccaacct aattttaatg attatgcagt ttgtttttct acgtaagtca
    25201 ctaaatcttg aaccgttttg attccgtctt ctgtttcgat tttcacgtca aattcatctt
    25261 caatttcatt gatgatttgg aataaatcca agctgtcagc ttctaagtct tcttggatgt
    25321 cagtagtcaa tttgatttct tcagtttctt ttcctaattc ttctgcgatg atttcttgaa
    25381 ttttttcgaa taccataata atatattcct ccgatattgt ttgcttttta tttttataat
    25441 ttaacagtct acaactccat gagaatactt ccccatgtta aacctccgcc atagcctgta
    25501 aatattactt tctgctgttt tcctaaggtg atctttccac tagaaactgc ttcatccaat
    25561 aagataggga tgcttgcagc agatgtattc ccatatttat ccatattggt caggaacttt
    25621 tcttgcggca tctttacttt tcgggcaatt ttctcaataa ttctgaggtt tgcctgatgc
    25681 aaaagcagat aatcaactgg tttgtctttc atgacttctt ggatattgtt tgcaacgtct
    25741 cgtacggcaa aatcaaaaat atctctgcca gtcatttgca aataatacga accttctgaa
    25801 tcttctgaat agaacggatt tttattttgc tggtagccag cggtcaatga gctacttctg
    25861 gtaccgtcag ctgctaattt ttctttcagc aattttttct gtggattcgc ttctaagaga
    25921 atcccacctg ctccatcgcc aaacaacaca gcagtcgtac gatcagacca atcgattagc
    25981 ttagatagtg tttcaccacc aatcaccagt cccttagttg agccgcagcg gatcattttt
    26041 tcagccatgc ttaacgcata aacaaatcct gagcacgcag cactgatatc aaaagcgata
    26101 gcatgagttg ctcccaattt cccttgtact tgtgctgcta cagatggtgt cgtgtaatcg
    26161 ggcgacattg ttgctactaa aataaaatcc aaatctttag ggtttagatt tttctgtttt
    26221 atcaattttt ccgccactaa aatgcataaa tcagaagtct cctgatttgt tacacatctt
    26281 cgttcacgaa tacccgtcct tgacgaaatc cattcatcag aggtatccat catcttggaa
    26341 agatcattat tagtaataat cttttcagga aggacgctgg ctgttgcagt aatcattcca
    26401 aattgttcca ttaaatatcc tcacttatag tcttgcaaga agtcgtgcaa gtttttcagt
    26461 gcatccagca aggcatgctg ttcctccgta gccattccgt ctaagatgtt cttgaccatt
    26521 tcacgatgaa aatgctgatg gacccgaaat agtaactttc cttttttcgt tagtcccaac
    26581 tttacaacgc ggcggtcatc ttcactgcgg atacgttcga catagccttt ttttactaac
    26641 ttgttaatag cagtggtcaa ggtcccaaca gtaattgata gttctttggc tacttctgaa
    26701 gttgtttttt tcttgtacat cccgattgcc tcgatcgtgt gcatctctgt gattgacaga
    26761 tctttgaatt gagatttttt cagctcagac tcttcgattg tcaaaatatc attgaagaca
    26821 ctaacaagat aatcatttac tgtttttaaa tctggttcca tgttccaccg tccactaatt
    26881 agtttgatta tcaaatcttt tgatgttcaa actatatctt aacttttttc aaaatgcaac
    26941 cttaaaagat cattttttta ctatatgagc tttttttaag attttactca tttaaaacaa
    27001 ctgataaatc agcatttata atttctttta ttagaaaaat catctatctc acatattttg
    27061 attatcaaag tattttctgt gaacagaact atagaattcc ctaaaacttg tccattattt
    27121 caaaatttgt tatagtcttt ttattcaaag cgtaaaagga gatcctatgt taaaagaaaa
    27181 aatgaaaact ctccccatgc tgccgggtgt ctatcttatg aaagaccgag atggtaaggt
    27241 catctatgtt ggtaaagcaa aacggctcaa aaatagagta agcagctatt ttcatcagaa
    27301 taaacagcat tcaaaaaaag tattacgtat gattcatcat ataacagatt ttgatttcgt
    27361 cgtagtagac acggaattag atgctttatt gctcgagtgt caattgatcc aacattaccg
    27421 tcctttttat aaccgacaaa tgaattattt cagtaattat aattatgtac atattaccaa
    27481 taagggattt gtattaacag atactcctac ggctcgaaca tacgggcctt ttcgtctcta
    27541 taaaaaaatg ccgtctattt tgcgtataat ggaagaaact tatcaaatgc cttggttgtc
    27601 tgaaattagt ttgttagctt tgcgagtcca gcttcctgac ctacaggaaa tgacttttga
    27661 acagaaaaaa aaggaactcc aagggctgtt tcaaggccga aataaaaagc tactgactta
    27721 cttgaaaaaa agacaacaac atttcatcta tcagttgaat ttcgaaaaag ccggtatgct
    27781 tcaaaaagat atcgaattag ttacttattt tattagacgg atccaggaac aaaagcagtt
    27841 tttaagaaca ccatcgctca ccttcagcat gccattagca gcagatgaga gtcagaaaaa
    27901 gcactacctc gtttgttatg gtcaacttgc agaaacaatg attacctctg gtgacgtttc
    27961 tcctgacttc tattatgaaa agaaagaagc tcatttatct ttgaaacgtc aattgtcaaa
    28021 agaagaaatc gatcctgtac aaatcttgat cagttaccgc aaaaaattag aaaaagaaca
    28081 gtttgaaatt gaactgttaa acaaaaaaga agctgaaaag caactaaatt agttgcttct
    28141 cggcttcttt taggcatctt caacttcaaa tgtcaattat cctaaaattt attggattaa
    28201 gtcatagatt tccatcgcta cgatatcgat attatcaaat tcgtattgct gatgagtatc
    28261 tgtttcagtt aattcaaatg tatcggtcgc tttatcataa gttactgtac acttctctac
    28321 gccttctttt tcaaaacggc gaacttgcag ttcattatct gctgactcga ccatagcctc
    28381 cagtcgtttt atgattgcga ctaattgtga atgtgtcatc ggaggtcctc cttttcttat
    28441 ttctgttata ttgtatcaaa agcatagcct acttgcattg attttctcta aaaaaactcg
    28501 ttttttatga ttttttttct ggtatttttc cttcaggcca ccaaagtttg atcaaagctt
    28561 gggttccgtc attctcaaaa cctttatctg ccaactcttc atatagtttc aatgcttggg
    28621 tagttgctgg caatacaaga tccatttttt ctgcttcttc taatgcaatc tttaaatctt
    28681 tgataaaatg tttgacaaaa aaaccaggtg tgtaatcttc ttttaaaata cgaggagaat
    28741 aattgcttaa agaccagtta gctgcacttc cacctgaaag cgtctcaatc actttcttta
    28801 aatcaagtcc gtttttttgt gcatagacaa gcatttccgt cattcctgtc atcgtacctg
    28861 caatcatgat ctgattagcc atttttgtat gctgaccttt cccagcagaa ccatgcaatg
    28921 taaatgtcgt accaaattct ttgaataaag gcagtacttg atcataaacg gcttcttctc
    28981 caccgaccat gatcgtcagt gtaccattct ttgctcctaa gtcaccaccg gataccggtg
    29041 catctaacgc atctgcgcct ttttcttttg ctgtttttgc aattttttct gccaggctgg
    29101 tgtacttgta gtcaaatcaa ctaaaatatg tcctgagata tctgcagaaa aaatccccga
    29161 ctgtccatag tagatttctt ctacatcttt ggggtaacct accattgtaa agagaatatt
    29221 actttgttct gctaccgctt ttggtgtatc tgcccagata gctccttctg ttactaaagg
    29281 atcagctttt gcttttgtcc gattatagac agctacttcg tgtcctgctt ctagtagatg
    29341 gcgtgcgaca gcacttccca tgacgcctgt tccgataaaa ccaagtttca ttttttcgac
    29401 ctcctaaaaa tcaaaaatcc ttgcttctat gctagtatac gctagaaaca aggatttttt
    29461 cataaaaaat gtttgaattt tatcttttaa atgaatttaa ttttattttg attcgttcaa
    29521 tcgagtcttc ttcttctttt cttcgcatct cttcttcatg ataaacaacc atgtcttgat
    29581 aaaattcgat catagcttcg ccgaaatgtg tagatgaaat atcataaagt ttagcttcta
    29641 aaacttttgg atctttagga acttcactgg aaatataatc aataagtgct ggtgcaaagt
    29701 cttcgtcggt ttcaaaagtt ttccctaatg aaggatgatc gaacaatcgg ttcaaataag
    29761 catttccttc cacgacacaa ggtgttccgg ctgccatcgc ttctgtataa gtaagccctt
    29821 gtgtttctga agtagaagca ctgacaaaat aatctgccgc tttataataa agtgctacat
    29881 cattgttgtc cacttctcct gtgaactgca caaattcttc taactgcagt tctgagatca
    29941 attcctctaa ctcttctaca taaggtcctt ttcctacgat aactaatctg gcgttaggat
    30001 aagcggagat gacctgagga aacccattaa tcaaggcttg gatatttttc tcataagaaa
    30061 tcctgcttaa agaaagaatc atcaaatggt tttcctgaag acctagtgac tcccttaatt
    30121 tgctgatatc ctgctgagtg atatcagaac gtttgaattt ttcaatgtcg ataccagtag
    30181 gaatcacgcg aagcggtgct attacaccat agctgcgtaa tgtatcaata acacgttcac
    30241 ttggacaaac tacacctgtc gtatgatttg caaataatct agacaagtat tttacatggg
    30301 attgcctgat cacttttcct ttaccgatat aatgaaggta gtcttcatac atcgtatggt
    30361 aagtatggat cagaggtatt ttcaacttct tagctaccat cttcccaagt atccctgctc
    30421 cgaattctgt atgggtatga ataagatcta gttctagttc tttggcaacc aaataagcat
    30481 accacatgcc tctaacaaca actcttcgat ccttgaaaga aataaatggt acacttggca
    30541 ttcgaatcac atctttttcc aacttatccg cattaggatc ggtcgttgta aaaatataga
    30601 cttcatgccc atgtctttct aattcttgtt ttaatgtttt gatcgatgta gcaactccgc
    30661 tgacttgcgg aaagtaggta tcggtaaaaa agccgatttt catgtttttc gcctccaaac
    30721 tagtccttga cctgatttac tttttttaat ttgctagaga ctttttcatt cagtacactt
    30781 tcgtatacat ttttaagttc ttcaccgatt ttattgatac ttcgctcttt tgctgtttga
    30841 taaccggctt gtgtcgtact cggtagttct tgattggtaa tctgttggat cagtcggata
    30901 aattcttcgt ttgtcttccc catatagcag ttcttgctat cttctaacca gccattatac
    30961 actggaatat ctcgaacaag tacgttttgt ttgcttgcta aagcttctaa aacaacgatc
    31021 ccttctgtct cttcataaga agggaaaaag aatagatctg cacctgaata cgcaccttct
    31081 atgatctctc cacgaatgta accaggaaac aggacatttt caggatgatc ttcttttaca
    31141 attttccgga tgttccgagg aatcgagtac atgggaacat gaccaaacca aataaatgtg
    31201 tattcgggca tcttcttagc aatttctacg aaatcaatga ttcctttacg ttcgaaaaac
    31261 aaaccgacac aaatgattac tttttgatcc ggagacagtt tgaaatattc tctgaacttt
    31321 tgttctttca ttggatcagg gtgatatttc tctaagtcga tcccattgga aatagcttga
    31381 atcggcacac ggataccata gctttccaac aactgttttg aataaggtgt tggcgtgatc
    31441 agatagtccg ctttttggta caatcctacc agatattttt ttacgatagg agaaagctgg
    31501 ttcgatctga taaaggaatt tcggaaatct tcctctgtag aatgcgcatg atagacaact
    31561 ttttttcctt ctcttctggc tttattgacc atattatgac tattgattcc gtatgtattg
    31621 atgtgcagga tgtcataatc ttctttttca tctagtgtat aagagatacc gacttccgtc
    31681 agagcacgtt tttgatgatc aagcgcacgc ccgattccag attttgcaag catcttttca
    31741 ctttcaaaat ataataatac cttcacctta cgtccccctt cacttatata gtataacata
    31801 gcctacaaac tgtcactcaa acttaagccc tatttttgtc tattcttact gacaaaaacg
    31861 ttttcttaaa aaagaacaaa aaaactggcg tagctctttt acaagccaca ccagcttttg
    31921 atacgttgtt tattttacat attcatgaac aagttcaacg acttcttcca tcatatcgca
    31981 ttctttcaat gcacgatcag caagttcagc cattttcgct gtgtccaagc gtttcattaa
    32041 actacgcgtt ttaagaatag atgtcgcact cattgagaac tcatctagac ccatacctac
    32101 tagcaatgga acagctgttt gatctccagc catttctcca cacatacctg cccattttcc
    32161 ttcagcgtgt gctgcatcaa ttacattttt gatcaaacgt aaaattgatg ggttatatgg
    32221 ttggtacaag taagaaacgc gttcgttcat acggtctgct gccattgtgt attgaatcaa
    32281 atcgttcgta cctacagaga agaaatctac ttcttttgca aacttgtctg ctaagactgc
    32341 tgctgctgga atctcgatca tgatgccgac ttggatcgta tcagatactt ccttgccttc
    32401 acttatcaat ttttgttttt cttcttcaaa gatcgctttt gctgcgcgga attctttgag
    32461 tgtagctacc atagggaaca tgatacgtaa attaccatga acagatgcac gaagcaatgc
    32521 acgcatttgt gtacggaaca tttcgtcgcc ttgttcagat aaactgatac gcaatgcacg
    32581 atatcctaag aatggattca tttcatgagg taattgcaga tatggtagtt ctttatctcc
    32641 accgatgtcc attgtacgaa caactacagg ttttccttcc ataccttcta atacagcttt
    32701 gtaagcttca tattggtcat cttctgttgg gaagtctgga gaatccatat acaggaattc
    32761 agtacggtac agaccaactg cttctccacc attattatgg acaccaacta gatcttttgg
    32821 tgttccgatg tttgcagcta attcaaaatg cttgccgtca gctgtgacag tttctgcatt
    32881 ttttaatttt tcccattctg ctttttgtgc tgcgtaatct gcacctgctt tttcaaattc
    32941 agctttttgt tcatctgtag gatcgatgat tacatcgcct tcgatcccgt tgacagccaa
    33001 aatatcgcct tctttgactt tagcagtaat ttcttttgtc ccaacgattg ctggaatttc
    33061 taaagaacgt gccatgatag cagaatgtga agtacgtcca ccaatgtctg ttacaaacgc
    33121 ttttacaaaa ttgcgatcta attgtgctgt atcacttgga gtcaagtcat gtgcaacaac
    33181 gaccacttct tcgttgatca ttgatggatt tggtaaagta acgcctaata agtgcgctaa
    33241 aatacgttta gcaacgtcac gaatatctgc agcacgctct tgcatataag cattatcttc
    33301 cattgcttcg aacataccga tatacatatc cgttacttct tttagcgctg attcagcatt
    33361 tacgctgttg tctttaatat tttgcttgat ttgacctacc atttctggat ctgaaagaac
    33421 catcaaatgg gcatcaaata cttgcgcttc tgcttcacct aagctttgcg ctgctttttc
    33481 acgaatttgc tgcaattctt cagtagattt agctaatgca gcatccaaac gagcttcttc
    33541 agccgcagta tcttctactg ttgccttact gaatgttaaa tccggttgaa ctagcaggta
    33601 agcttttgca acggctatac cgtcactagc ggcaatccct tttagcattt caaccattat
    33661 tcagataagc cttctttctt cattgtttca acgattgctg ccattgcatc agcttcgtca
    33721 gcaccttctg cagtaatagt aacatcagaa ccttgaccta cacctaaaga cataacgccc
    33781 atgattgatt tcaagtttac tgatttacct ttatactcta agtttacatc agagttaaat
    33841 ttgcttgctg tttgtactaa tagtgtagct ggacgagcgt ggatcccagt ttctgctact
    33901 acgtgaaatt ctttcttttc catattaatc ggtctccttt aagtgagtgt tgtttttatg
    33961 tgagggtttt cttttttagt aattcagaga atcctaacaa atgccaccct ttcatctgta
    34021 aagattaaca ttttttttcg ttagatacaa cagttttcat tcaatttcag ataaaagtat
    34081 tcaaattatt ccggattctt tttcatcctt ttcttcacat tttataacaa acgtgtttat
    34141 ttcctttttc ttgtcgcttt ctcttctcct gacgtttatc attctttcct ttgaccctct
    34201 ttgttttcat atgaaacgaa ttacttgctt ccctttttat ttttagtata ataagagtca
    34261 aaggtcagga atggtcaata gagatttcac cttttctacc tcataaatct cttaaaaaac
    34321 taggggttat tcttttacct catgaaaaac gagatgtaaa atatggatgt acccattttt
    34381 gtgaaaggac gtgagtgatt catgctttgt caaaactgcg gcaagaatga ggcaacgatt
    34441 cacttgtatg ccactgtcaa tggtcagcgg acacaactcg attactgtca gagctgttat
    34501 caaaaaatca aaaatcaaca aaatggaggt ctgactaata tggcccaaaa tgatccattt
    34561 ggatttggca gtttggacga tcttttccgt tccatgtctc gccaaatgca acagcaagga
    34621 aattatgagc aaacaccacc aacacaattt ggcggaaaca acaacttcaa tggtggtcag
    34681 ccacctcaag gcaatacaga aggtttattg ggagaatacg gaatcaatat cacagaacaa
    34741 gcacgacaag gtgatatcga tcctgtgatc ggacgcgatg acgaaatcaa acgagtcatt
    34801 gaaattttaa atcgccgaac caagaacaat cctgtcttga ttggagaacc aggtgttggt
    34861 aaaacagcag ttgttgaagg tttagcacaa aaaatcgtag atggtgatgt accgcaaaaa
    34921 ttaatggaca aagaagtcat tcgtttagat gtcgtatctt tagtccaagg tactggtata
    34981 cgaggacaat ttgaagaacg tatgcaaaaa ttgattgaag agatccgtca agcagaaaat
    35041 gtgatcctat tcatagatga agttcacgaa atcgtcggtg ctggttctgc tggtgatgga
    35101 aatatggatg ctggtaatat tttaaaacca gctttagctc gaggagaatt gcaaatggtt
    35161 ggtgctacta ccttaaatga gtaccgcatc atcgaaaaag acgctgcttt agaacgtcgt
    35221 atgcaacctg tacgggtaga tgaaccaact gtcgaagaaa cgatcagtat tttgaaaggt
    35281 ttgcaaaaac gttatgaaga ctatcatcat gtaaaatata ctgatgaggc catcgaagca
    35341 gccgcaactt tatccaatcg ttacgtccaa gatcgcttct tgccggataa agccatcgat
    35401 ttgctagatg aaacaggttc taagaaaaac ttgacgatcc aaatcgttga tcctaaaaca
    35461 atcgagaaaa aactgcaaga agcagaagaa caaaaagttt tagcttctcg agaagaagat
    35521 ttcgaaaaag cagcttatta tcgcgatcag atcaataaac ttcaaaaaat gaaagaacgt
    35581 cagctcactg aagaggaaac acctgtgatt accgaaaaag atatggaaaa aatcgttgaa
    35641 caacggacag gtattccagt gggtgaatta aaagaaaaag aacagacaca actgaaaaac
    35701 ttagcagatg acttgaaagc tcatgtcatc ggacaagata acgctgtaga tcgagtagcg
    35761 aaagcgattc gccgaaaccg tgttggatta aacaaacaga atcgtccaat cggctccttc
    35821 ttatttgttg gaccaactgg tgtaggtaaa acagagcttg cgaaacaact tgcctacgaa
    35881 ctattcggtt cacaagattc aatgattcgt ttcgatatgt ctgaatatat ggaaaaacat
    35941 agtgtctcta aattgattgg ttctcctcca ggttatgtcg gatacgaaga agctggtcaa
    36001 ttaacagaaa aagtacggag aaatccttac agtcttgtct tgttagatga agtagaaaaa
    36061 gctcatccag atgttttgca tatgttcttg caaatcttag atgatggtcg attgacagat
    36121 gcacaaggaa gaacggtcag ctttaaagat acgatcatca ttatgacaag taatgccgga
    36181 actggtaaag tagaagcaaa cgttggtttt ggtgcggcac gcgaaggtgt tactcgttct
    36241 gtattgaacc aattaaacaa ctactttaca ccagaatttc tgaaccgttt tgacgggatc
    36301 atcgaattca gtgcattatc aaaagaaaac ttgatgacaa tcgtcacact aatgctagac
    36361 gatgtcaacc aaatgctggc agcacagcaa cttcacatcg aagttcctac gaatgtcaaa
    36421 gaaaaattag ttgacttagg ctatgatcca tctatgggtg ctcgtcctct tcgacgcacg
    36481 atccaagaac aaatcgaaga tggtatcgct gaattttatc ttgatcaccc ttctatccat
    36541 gagttgaaag caaaactgga taaagatgga aaaatcatcg ttacttctaa accagaacgt
    36601 ttggctaaag aatcagccga agaaacggct gaataaatcc agttttatag cgtgagacgg
    36661 tatttctttg agaaatgccg tctcattctt ttttcagaaa agtcaaaata ctgttcattt
    36721 tataaaaaag aaattataat aagagaaaca agcaattaaa aatacataaa agaggtgagc
    36781 gccatgaaat tggtaaatgt aacaaacagc cattcccgtc ttgtattaaa ccagcttgaa
    36841 aacactgatg cccatctagt caaggtctac acagccggaa ataccactat tgtctacact
    36901 gaagcccctg aacataatga aattctgctg atcaatgaca aacgtaaaat ccagccgaaa
    36961 gaaatcgaag atgctaaaaa ttatttctta gataaaatca aagacgccgt ttaccacgag
    37021 gatgaaatca aagtaatcga attggcaggc cttgttgaaa tctcgattcc aaaacatgaa
    37081 ttaggcgcta cattagccgt gtaaacaaat tttgaacata at
//
LOCUS       DBNICI010000007        35540 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.007, whole genome shotgun sequence.
ACCESSION   DBNICI010000007 DBNICI010000000
VERSION     DBNICI010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 35540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.007"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..1254
                     /locus_tag="AC1ZCC_000313"
     CDS             <1..1254
                     /locus_tag="AC1ZCC_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HIQ1628600.1"
                     /translation="AEGEIVKWFVKPGDTINEDDTLLEVQNDKSVEEIPSPVTGTVKN
                     VIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETSGSASVVEAADPNKR
                     VLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPSSAPAKSEAPEAAAP
                     KEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVNSKHTAPHVTLHDEV
                     EVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLNASIDDANQEIVYKH
                     YYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDGKLSAEDMRNGTITI
                     SNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGRVMKLSLSFDHRIVD
                     GATAQQAMNNIKRLLADPELLMMEG"
     gene            1262..2668
                     /gene="lpdA"
                     /locus_tag="AC1ZCC_000314"
     CDS             1262..2668
                     /gene="lpdA"
                     /locus_tag="AC1ZCC_000314"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIQ1628601.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2830..3105
                     /locus_tag="AC1ZCC_000315"
     CDS             2830..3105
                     /locus_tag="AC1ZCC_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HIQ1628602.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3111..3878
                     /locus_tag="AC1ZCC_000316"
     CDS             3111..3878
                     /locus_tag="AC1ZCC_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIQ1628603.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            4046..5875
                     /gene="typA"
                     /locus_tag="AC1ZCC_000317"
     CDS             4046..5875
                     /gene="typA"
                     /locus_tag="AC1ZCC_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIQ1628604.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6136..7908
                     /gene="recQ"
                     /locus_tag="AC1ZCC_000318"
     CDS             6136..7908
                     /gene="recQ"
                     /locus_tag="AC1ZCC_000318"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HIQ1628605.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            8022..8324
                     /locus_tag="AC1ZCC_000319"
     CDS             8022..8324
                     /locus_tag="AC1ZCC_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HIQ1628606.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8327..9490
                     /locus_tag="AC1ZCC_000320"
     CDS             8327..9490
                     /locus_tag="AC1ZCC_000320"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIQ1628607.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9516..12944
                     /locus_tag="AC1ZCC_000321"
     CDS             9516..12944
                     /locus_tag="AC1ZCC_000321"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HIQ1628608.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12965..14089
                     /locus_tag="AC1ZCC_000322"
     CDS             12965..14089
                     /locus_tag="AC1ZCC_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HIQ1628609.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            14070..14516
                     /locus_tag="AC1ZCC_000323"
     CDS             14070..14516
                     /locus_tag="AC1ZCC_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HIQ1628610.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14500..14838
                     /locus_tag="AC1ZCC_000324"
     CDS             14500..14838
                     /locus_tag="AC1ZCC_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HIQ1628611.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14957..15517
                     /gene="rsmD"
                     /locus_tag="AC1ZCC_000325"
     CDS             14957..15517
                     /gene="rsmD"
                     /locus_tag="AC1ZCC_000325"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIQ1628612.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15514..16005
                     /gene="coaD"
                     /locus_tag="AC1ZCC_000326"
     CDS             15514..16005
                     /gene="coaD"
                     /locus_tag="AC1ZCC_000326"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIQ1628613.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15995..17038
                     /locus_tag="AC1ZCC_000327"
     CDS             15995..17038
                     /locus_tag="AC1ZCC_000327"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HIQ1628614.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17197..17925
                     /locus_tag="AC1ZCC_000328"
     CDS             17197..17925
                     /locus_tag="AC1ZCC_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIQ1628615.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            18041..18286
                     /locus_tag="AC1ZCC_000329"
     CDS             18041..18286
                     /locus_tag="AC1ZCC_000329"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIQ1628616.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18283..18501
                     /locus_tag="AC1ZCC_000330"
     CDS             18283..18501
                     /locus_tag="AC1ZCC_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HIQ1628617.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18637..19176
                     /locus_tag="AC1ZCC_000331"
     CDS             18637..19176
                     /locus_tag="AC1ZCC_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HIQ1628618.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19169..21442
                     /locus_tag="AC1ZCC_000332"
     CDS             19169..21442
                     /locus_tag="AC1ZCC_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HIQ1628619.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21480..22526
                     /gene="holA"
                     /locus_tag="AC1ZCC_000333"
     CDS             21480..22526
                     /gene="holA"
                     /locus_tag="AC1ZCC_000333"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIQ1628620.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22615..22869)
                     /gene="rpsT"
                     /locus_tag="AC1ZCC_000334"
     CDS             complement(22615..22869)
                     /gene="rpsT"
                     /locus_tag="AC1ZCC_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIQ1628621.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23269..23538)
                     /locus_tag="AC1ZCC_000335"
     CDS             complement(23269..23538)
                     /locus_tag="AC1ZCC_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628622.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23800..23928)
                     /locus_tag="AC1ZCC_000336"
     CDS             complement(23800..23928)
                     /locus_tag="AC1ZCC_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628623.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(24008..24463)
                     /locus_tag="AC1ZCC_000337"
     CDS             complement(24008..24463)
                     /locus_tag="AC1ZCC_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HIQ1628624.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24601..26529)
                     /locus_tag="AC1ZCC_000338"
     CDS             complement(24601..26529)
                     /locus_tag="AC1ZCC_000338"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIQ1628625.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26726..27559)
                     /locus_tag="AC1ZCC_000339"
     CDS             complement(26726..27559)
                     /locus_tag="AC1ZCC_000339"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIQ1628626.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27751..28455)
                     /locus_tag="AC1ZCC_000340"
     CDS             complement(27751..28455)
                     /locus_tag="AC1ZCC_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIQ1628627.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28478..29104)
                     /locus_tag="AC1ZCC_000341"
     CDS             complement(28478..29104)
                     /locus_tag="AC1ZCC_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIQ1628628.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29430..31805
                     /locus_tag="AC1ZCC_000342"
     CDS             29430..31805
                     /locus_tag="AC1ZCC_000342"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HIQ1628629.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31829..32320)
                     /locus_tag="AC1ZCC_000343"
     CDS             complement(31829..32320)
                     /locus_tag="AC1ZCC_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HIQ1628630.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32507..33427
                     /locus_tag="AC1ZCC_000344"
     CDS             32507..33427
                     /locus_tag="AC1ZCC_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIQ1628631.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33581..33925
                     /locus_tag="AC1ZCC_000345"
     CDS             33581..33925
                     /locus_tag="AC1ZCC_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HIQ1628632.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34115..34615)
                     /locus_tag="AC1ZCC_000346"
     CDS             complement(34115..34615)
                     /locus_tag="AC1ZCC_000346"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1628633.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35143..35226
                     /locus_tag="AC1ZCC_000347"
     tRNA            35143..35226
                     /locus_tag="AC1ZCC_000347"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35177..35179,aa:Leu,seq:cag)
BASE COUNT        11819 a         6184 c         7318 g        10219 t
ORIGIN      
        1 gcagaaggtg aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat
       61 acattactag aagttcaaaa tgacaaatca gtagaagaaa ttccgtcacc tgtcacaggg
      121 acagttaaaa acgtgattgt tccagaaggc actgtagcaa atgttgggga tgtattagta
      181 gaaatcgatg ctcctggaca caatagtgca ccgtcaacta gtgcgccatc agctgaagca
      241 ccaaaagaaa aagtggaaac atcaggttca gcaagtgtcg ttgaagctgc cgatccaaat
      301 aaacgtgtat tggctatgcc atctgttcgt caatttgccc gtgaaaaaga tgtagatatc
      361 tcacaagtaa ctgcaactgg aaaaggtggc agagtaacaa aagaggacat cgaaaacttc
      421 ttggcaggtg gtccaagcag tgcaccagcg aaatcagaag cgccagaagc ggcagcaccg
      481 aaagaagcag ctcctgcagc agaaagcaaa ccagctgcac cagcaaaacc attcaagtcc
      541 aacttaggag acttggaaga acgtgtagca atgacgccaa cacgtaaagc tatcgctaaa
      601 gcaatggtca acagtaagca tacagctcca cacgtgacat tgcatgatga agtagaagtg
      661 tctaaactat gggataatcg gaaacgattt aaagaagttg ctgctgcaaa tggtacgaaa
      721 cttactttct tgccatatgt cgtgaaagct ttaacagcga ctgttaagaa atacccagta
      781 ttgaatgcat ctatcgacga tgcaaaccaa gaaatcgttt ataaacatta ctacaatatc
      841 ggtattgcaa ctgatacgga tcacggacta tatgtaccaa atgtgaaaga tgcagaccgc
      901 aaaggaatgt ttgctattgc agacgaaatc aatgagaaag caaaattagc tcatgacggc
      961 aaactatctg cagaagatat gcgcaatgga acaattacga tcagcaatat cggatctgta
     1021 ggtggcggtt ggtttacacc agtcatcaac tatccagaag tagctatttt aggtgtagga
     1081 acgattgcac aacaaccaat cgtaaatgct gaaggcgaaa tcgttgtcgg tcgtgtgatg
     1141 aaactgtcat taagtttcga tcaccgtatc gtagatggag caactgcgca acaagcaatg
     1201 aataatatca aacgtctttt agctgatcca gagctattaa tgatggaagg atgataaaca
     1261 aatggtagtt ggagattttg ctgtagaatt agatacagtt gtaattggtg cgggacctgg
     1321 tggctatgta gctgccatcc gcgctgctga aatgggacaa aaagtagcaa tcattgaaag
     1381 agagtatatt ggcggtgttt gtttgaatgt cggctgtatt ccttcaaaag cactgatagc
     1441 tgcaggacac cattatcaag agtcactaga ttctgaaatg tttggagtaa ctagtgaaaa
     1501 cgtcaaacta gattttgcaa aaacacaaga atggaaagaa aacaaagttg tccatacgtt
     1561 gacttctggt gttggtttcc tattaaaaaa acataaggtt gaaacaatcg aaggagaagc
     1621 gttcttcgta gatgatcaca cactacgcgt cattcatcca gattcagctc aaacttattc
     1681 gttcaataac gcaatcatcg caacaggttc ccgtccaatc gagattccag gatttaaatt
     1741 cggaggacgt gtgttagatt ctactggcgg attagcattg aaagaagtac ctaagaaatt
     1801 tgtgatcatc ggtggcggtg tgattggtgc cgaattgggc ggagcgtacg caaacttagg
     1861 agcagaagta acgattcttg aaggctcacc acaaatttta ccaacttacg aaaaagatct
     1921 agtaaaatta gtagaagatg atttcaagaa aaaaggcgta acagtcgtaa cgaatgcaat
     1981 ggcgaaagaa gcagtcgata acggtgacag cgtgacagtg aaatatgcag tggacgggaa
     2041 agaagaatcc gttactgctg attatgtgat ggtcactgta ggtcgtcgtc cgaatacaga
     2101 tgatatgggc ttagaacaag cgggtgtcga agtaggagaa cgaggattga tcacggtgga
     2161 taaacaagga cgtacgaacg tacctaacat ctatgccatc ggtgatatcg ttccaggggc
     2221 ggcattagca cacaaagcca gttatgaagc aaaaatcgct gctgaagcaa tctctggcaa
     2281 aaaagtagct gtagattata aagcaatgcc ggcagtagcc ttcactgatc ctgaattggc
     2341 ttctgtaggt atgacgatca aagaagcaaa agatgctggt atcgaagcaa aagcatataa
     2401 attcccattt tcagggaacg gtcgggcatt atcgcttggt aaaacagaag gattcatccg
     2461 tctagttacg acgatcgaag ataacgtgct tattggcgcg caaatcggtg gagtcggcgc
     2521 aagtgatatg gtctctgaat tagctttagc aatcgaatca ggaatgaatg ctgaagatat
     2581 cgccttgacg attcatcctc atccatctct tggcgaaatc gtcatggatg cttcggaatt
     2641 agcattaggt ttaccaatcc atatttgatt tgaataatac aggcgaaacg caactgaaaa
     2701 atcagttgcg ttttgttttt tagaattatc aataatatta ttatgtaaac ttaaaaatat
     2761 gacaacttct tggtttatta ccacttccta tgctacactg aagaggaata tgaaatagga
     2821 gagttgaaga tgcaagaata tcagtatcca cttgatttag attggacaac agacgaaatg
     2881 gtcattgtaa tgaagatgtg ggaagatctg gaacgtgcga atgaaaaagg gataaacaat
     2941 gaagcttttt tgaagactta tcagcagttt aaaactgttg tcaaatccat tggtgaggaa
     3001 aaacgattgg gacgggaatt tgaaaaagct tccggttatt ctttatatcg tacagtgcag
     3061 caagcaaaaa aaaatccaaa caaaactttg agaatgaacg ggtgagataa atggaacaga
     3121 tgattgatca tatcaagtcc tggcttgaag aagctggtga gaaaatcaaa tcttctcaac
     3181 aaacattaac aatctcgcaa aaatcaaatc gaacggatct agtaacaaat gtcgatcgaa
     3241 acatccagaa ttttctaatc gaaaaaatcc attcctttga tccagaggcg aaaatattgg
     3301 cagaagaaga agggtgtaat caattatcgg atctcgatgg aagagtcttt atcatcgatc
     3361 ctattgatgg caccttgaat tttgtgattg aaggggaaaa tttttgtatt atgctcgctg
     3421 tttatgaatc cggcgttgga aagcttggtt tcatttatga cgtgatgaga aatgaacttt
     3481 tttggggtgg aaaaggaata ggcgtgtata aaaatgatga gccattgcct agaccagcaa
     3541 ataaatcctt atcggaaggc ttacttggtg tgaatagtta tcttttcggt cataataaat
     3601 atggaatccg agaaattgcg gaaagcagca tggggacacg tatgtacgga tgcgctggtc
     3661 ttgaactagt ggcaatcttg aaaggaaacc acattgctta tatttcaaac cttagccctt
     3721 gggattatgc agcgggaaat gtcttattgg aagagtttgg tatggtaaca agcggtcttt
     3781 ttggtactcc tttgacattt gaaaaaaggg aatactattt agcagggact tcttgtgcat
     3841 atgcagaaat cgcagacctt ttgaaaaaag aaggctgaaa aaaaggtaga aatcattcac
     3901 aaaaatgata aaaacgagca aaagattaat tttctcgaaa aaatgaaaac attttactat
     3961 atttcatgaa aaaagtctgc tataattagc agtcgagtaa aatttgccct gtcataaaga
     4021 accaataaaa aaggagttag ttacattgaa ttatagaaac gatatccgta acgtcgcaat
     4081 tattgcccac gttgaccacg gaaaaacaac attagttgat gaactattaa aacaatcaga
     4141 tacattagac gcacatacgc aattgcaaga acgtgcgatg gactcaaatg cgctagaaaa
     4201 agaacgtggt atcacgattt tagccaaaaa cacagctgta gactataaag gtattcgcgt
     4261 aaacatcatg gatactccag gacacgcgga cttcggtggc gaagtagaac ggatcatgaa
     4321 aatggttgac ggcgttgtgt tggtcgtaga tgcgtatgaa ggaacaatgc ctcaaacacg
     4381 ttttgtattg aaaaaagcac tggaacaaca tatcacacca atcgttgtcg taaacaagat
     4441 tgacaaacct tctgctcgtc ctgagcatgt cgtagatgaa gttcttgaac tattcatcga
     4501 attaggagca gatgatgatc agttagactt tcctgtgatt tatgcatctg cactaaatgg
     4561 tacgtcaagc ttgtcagatg atccagcaga tcaagaacca accatggcac ctatttttga
     4621 tacaattatt gaaaaaatcc ctgctcctgt cgataacagc gacgaaccat tacaattcca
     4681 agtttcatta cttgactaca acgattatgt aggtcgtatc ggaatcggac gtgtatttag
     4741 aggaacaatc aaagtaggag accaagtagc attaattaaa ttagacggaa cagttaagaa
     4801 attccgtgta acgaaattat ttggtttctt tggactaaaa cgtctagaga ttcaagaagc
     4861 aaaagctgga gacttgattg ctgtctcagg tatggaagat atctttgtcg gtgaaacagt
     4921 tactcctgtg gatcatcaag atgcgttacc tatccttcac attgacgaac caacattgca
     4981 aatgactttc ttagttaata attcaccatt tgccggtcga gaaggtaaat tcgtaaccgc
     5041 tcgtaagatt gaagaacgtt tgatggcaga acttcaaaca gatgtatcct tacgagttga
     5101 accaacaaac tctccagatg catggacagt ttctggtcgt ggagaattgc atttgtctat
     5161 cttgattgaa aacatgcgcc gtgagggata cgaattgcaa gtatctcgtc cagaagttat
     5221 tgaaaaagaa atcgacggtg taaaatgcga accgtttgaa cgcgtgcaaa tcgatacacc
     5281 agaagaatat atgggtagtg ttatcgagtc cttgagctta cgtaaaggtg aaatgcaaga
     5341 tatggtccat acaggaaacg gacaaatccg tttgacattc ttaacacctg cccgcggttt
     5401 gattggttat tcaactgaat tcctttcaat gacacgtggt tacggaatca tgaaccatac
     5461 ttttgaccaa tatctgccaa tgcttcctgg tcaaattggc ggacgtcacc aaggggcgct
     5521 tgtttcgatc gatacaggaa aagcaacgac ttattctatc atgagtatcg aagaacgcgg
     5581 tacagtcttc gtagaaccag gtacagaagt atatgaagga atgattatcg gggaaaacag
     5641 ccgtgataat gatttgactg tcaacatcac gaaagcaaaa caaatgacaa acgtacggtc
     5701 tgctactaaa gaccaaacat ctgtcatcaa aaaaccaaaa caattgacgt tggaagaatc
     5761 tttggaattc ttgaatgatg atgaatattg cgaagtaaca ccagaaagca ttcgcttgag
     5821 aaaacaaatt ttagagaaaa atgctcgtga aaaagcatct aaaaagaaaa aataatctgt
     5881 aaaaggatga agagagaaac cagcttgtta taaaaaatag ctggtttctt tttttgtcca
     5941 actcttttct gtggagaagt catctttgtc aatggacata aattcagaaa atacgccgaa
     6001 agacgtttaa aagactttta aaaaacttat ttctagagag tttttgtttc ataataagat
     6061 aatgtttcac gaaaggagat caacttgtcc ttcgaagaaa atcgaagtaa aatggcaaga
     6121 gaaaggagcg ggaagatgaa cgacttatta aaacaatact ttggctatga tgagtttaga
     6181 cctggtcaaa aagaaattat ccagaaagtc attgatcagg aaaatgtatt aggaatcatg
     6241 cctaccggaa gtgggaaatc aatctgttac cagcttccag ctttactatt agatgggctt
     6301 actgtggttg tgtccccttt aatctctttg atgaaggatc aggtggacgc ggcgaatcaa
     6361 ttaggtatac ccgccacttt tatcaatagc tcgttagatg gttacgaaac agctagacgc
     6421 ttccaagaaa tcgatagaca gcagtaccgt ttactttata tcgctcctga acgttttatc
     6481 atgccggatt ttatccaagc aatgaagcga tggaacgtcc gtatgatcgc tattgacgag
     6541 gcacattgta tctctcagtg ggggcatgat ttccgcccaa gttatttaca gatggcgaac
     6601 cagctagatc aactgccaaa tcgtcctgta attgttgctt tgacagctac agcgactgtg
     6661 caagtagctg ctgacataaa aagactttta aaaattccag aaaacaacca tattcagact
     6721 ggttttgaac gagaaaatct acgatttcaa gtaattaaag atcaaaaaaa agaacaatat
     6781 ctaatcgaat atttaaagat caacaaaaat caatctggta tcatttatgc agcaacgaga
     6841 aaagaagtag accgtctcta tcatttgctt aaaaaattcg atttttccgt gggtagatat
     6901 catggtggtc taaatgagaa tgagcgaaca gaaatgcaag aagccttttt atacgatcgc
     6961 ttgcagctga ttgtagcgac aaatgcattt ggtatgggta taaataaaag caatgtccgt
     7021 tttgttatcc attatcaaat tcctggctct ctagaagcat actatcagga agctggtcgg
     7081 gcgggaagag atggtctttc cagtgaagcc atcctgcttt tctctcctca agatatccaa
     7141 gtccaaaagt tttttatcca gcagtcccaa agagaagaag ggcaaaagca aaaagaatat
     7201 gaaaaattaa aagcgatgac agaatacgtg tatattgagt cctgtcttca gcaatacatc
     7261 ctaaactatt ttggcgagac aagcagtccg tgcaaccgtt gtggtaactg tttagatgac
     7321 agagaaatag tagaagtaac gacagaagca cagatggttc tttcttgctt aaaacgtatg
     7381 ggtgagaact atggtaaaca aatgctgatg aaagtcttag caggatcgaa agaacaaaag
     7441 ctgcaagctt taggttttgg acatttatct acctatggac tgatgaaaaa ccaatcgcaa
     7501 aaagaaacga tgcagttaat tgaatatttg atatcgaatg gctatcttct gaccatcaat
     7561 ggtgagtatc ctgttttgaa agtaacagaa agagggatac aagtattgaa aggacaagaa
     7621 tctgtttatc gaaaggaacc caagaaagtc cagcagctat cggatgaaga aaccgataca
     7681 ctatttgaag tgctgcggga actgcgaacg gatctagcat cagaagcagg cgttcccccg
     7741 tatgtcgtat tttcagattc cacattgaaa gaaatgagcc gaatacgtcc atcaagcaga
     7801 ttggagatgc ttcagataaa aggagttggc caaagcaagc ttgataaata tggcgaagcc
     7861 tttttatctc ggataaaaaa tgcggactct aacgttctgg ataagtagaa agaacgaata
     7921 cgtaaaaatg aataaaaaca actggttaaa tctgtttttt ttgctcctta tgaaaatcaa
     7981 atatgttata attaaggaat tagaatggga ggtaagcaca gatggattct ttgtcgaaaa
     8041 aacttgcgat ccaagtatta aatgatgaag cagaacggat caaacgatta atccgaaatc
     8101 aaaacaacag tctctgtatc tcacaatgca aagccttcga agaagtggta gatacacaga
     8161 tgtacggctt ttcccggcaa gtcggttttg ctgtccgtat aggagtaatc ggaaagaacg
     8221 agggccagct gatgttaagt gagctagaac gagaattgaa tcagttatat agtgaggttt
     8281 accaagaaat ttatgataaa aaagaagttg gcaaggagga atagacttgc ctaagaaagt
     8341 gaaaaaaagg catctattag actatagcat cttgattccc tatttaattt tatgtgtatt
     8401 gggattgatc atggtatata gttctacgtc gtatctatta ttagaaaacg gacaaaatcc
     8461 gtccgcatca gtcattaacc agagtatttt ttgggtgctt agcttgattg ctatcgcact
     8521 actttataaa atgaaaacag atgttttgaa aaaccagcgt ttgatcatgg cagccatcgc
     8581 agttttgacg attttgctgc tgattgttgt gttttttggt aaggagatca atggggcgaa
     8641 aggttggctg caaattgcag gattctcgat ccaacctgcc gaatacctaa aaatcatttc
     8701 tatctggtac ctgtctctga ctttatctaa aagacaaaac agtgtacaaa aagattttct
     8761 tgggactgtg aaacgtccac ttgcaatggt tatcggactt actgcattag tcgctattct
     8821 tcccgatttt ggtaatgcgg cagttatctt tttgattatt ttggttctgc ttttagccag
     8881 cggagtcaat tatgtgtata ctttgattgt aggagtagga ggcttttgtc taagtacttt
     8941 tacgatctgg ctgatcaata tcacaaacgg aaagatctta cccggacgtt tacagtatat
     9001 ctataatcga tttgctattt accaaaatcc tttctcagac gaattaaata aagggcatca
     9061 actagtcaac ggctactatg ccatgttcaa cggaggacta tttggcaggg gattaggtaa
     9121 cagtatccag aaaaaaggat ttttacaaga agcacaaacc gattttattt atgcaatcgt
     9181 agtggaagaa ctgggagtga ttatggggat tttgatatta gcgttgctat tctttatgat
     9241 tgtgcgcatt gtccttgtag gtatccgttc aaaagatccg ttcaattcgc ttttgtgcat
     9301 tgggatcggc gcaatgtttt tgatccaagt atttgtcaat cttggaggaa ttactggcgt
     9361 tattcctttg acagggatca cgttcccatt tttaagtcaa ggaggctcca gtttgctgat
     9421 gctttcgatt tgtgtcggct ttgtcctcaa catcagtgcg gatgaaaaac gcaagagctt
     9481 aggtttataa taaaaaaaga tgtaggagtg gttcgatgaa aaaagtttta gttgctaaca
     9541 ggggtgaaat cgctgtacgt gtatttcgtg cttgtacaga attaggaatt aaaactgttg
     9601 gaatctatgc agaagaagat gaatattccg tacaccgttt caaagcggat gaagcatatt
     9661 tagtaggaca aggaaaaaaa ccgattgatg cgtatctaga tattgaaggg atcatttcta
     9721 tcgcgaaaga atgcggagct gacgctattc atccgggtta cggtcttttg tcggaaaact
     9781 tgaattttgc gcaacggtgc gaagaagaag gcatcatttt tgttggaccc aaactgcatc
     9841 atttagatat ttttggagat aaaatcaaag caaaagctgc agctatcgaa gcggggattg
     9901 cttcgattcc aggcacagat ggaccaatcg cttctataga cgatgccttg gagtttgcga
     9961 aacaatacgg ttatccgatt atgatcaagg cagctctagg cggcggcgga cgcggcatgc
    10021 gtgtagctca cgatgaaaag agtgcaagag agggctacga acgtgcgaaa agcgaagcaa
    10081 aagcagcctt tggtagtgat gaagtgtatg ttgaaaaata catcgctaat cctaagcata
    10141 tcgaagtaca gattctcggg gatacgcatg gaaacgtcat ccatcttttt gaaagagatt
    10201 gttctgttca gcgccgtcac caaaaagtag tagaagtagc gccttgtgtc tctatgaatg
    10261 aacagcaacg tcaaaaaatc tgccaggcag ctgtccaatt gatgaaatat gtcgggtatg
    10321 tcaacgcagg tacagtagaa tttcttgttg aaggagatga tttttacttt atcgaagtca
    10381 accctcgcgt ccaagtggaa catacgatca cagaaatgat tacagatatt gatatcgtaa
    10441 caacgcaact gttgattgct caaggtttgg atctgcataa agaaatcgga ttgcctcagc
    10501 aagaaggaat taaattaaat ggttcagcca ttcaatgccg aatcactaca gaagacccgt
    10561 tgaataactt tttaccagac acagggaaaa tcgatacgta tcgttcacct ggtggttttg
    10621 gcgtacgtct ggatgtcgga aatgcttacg cgggctatgt agtgacaccg tattttgatt
    10681 cattgctagt aaaagtatgt acgcatggtg ctacctttga aacagccatt cagaaaatgg
    10741 aacgttgctt gaaagaattt agaatccgtg gagtgaaaac aaacattcca tttatgctca
    10801 atgtcatcac acatcctgaa ttccaatcag gaaatgcaaa aacaaccttc attgatagta
    10861 cagcaacatt atttgaattt cctcgactac gagaccgcgg aaacaaaacg atgaaatata
    10921 tcggtgaaat tactgtgaat ggttttccag gtatcgaaag tggcgaaaaa ccattttatg
    10981 aagaaccacg tatgcctaaa gatctaatca caagaactga ttacgtaacg gctaagaatg
    11041 tattagatgc gaagggtgcc gatgctttgg tcgaatggat caaaggacag gaaaatttgt
    11101 tgttgacgga tacgacattt agagacgccc atcaaagtct gctagcaact cgtgttcgga
    11161 caaaggattt caagcagatt gcacgtttga ctggagaagg tcttcctgaa ctcttttcaa
    11221 gtgagatgtg ggggggagca acatttgatg tggcttaccg ctttttgaat gaagatcctt
    11281 ggcaacggtt gagaaaaatt cgttctttaa tgcctaatac gttgctgcaa atgcttttca
    11341 gaggttcaaa tgccgttggg tattctaatt atcctgacaa tgtattggtg gaatttgtca
    11401 aagaagcagc agcgcaagga atcgatgtat ttcgaatctt tgatagtttg aactggactc
    11461 cacaaatgga aaaaagtatc caagctgtac gggatactgg taaaatcgca gaggcagcta
    11521 tttgctatac tggcgatatc aacgatccaa gtcgggcaaa atacaatgtt cagtattata
    11581 aagacatggc aaaggaacta gaacaactgg gtgcgcatat cattgcaatc aaagatatgg
    11641 ctgggctgct taaaccgcag gcagcttatc gtttgattag tgaattgaaa gaaacgacag
    11701 atctgccaat ccatctgcat actcatgata ccagcgggaa tggaatcatc acttattctg
    11761 ctgcttcaaa agcaggagta gacatcgttg acgtggcaat gagtgcaatg agtgggaata
    11821 ctagtcagcc tagcatgagc agcttgtatt atgcactggt caatggtcca cggctgccag
    11881 aaatcacgat tgaaaatgca cagaaattaa accattattg ggaagatgtg cgcatgtatt
    11941 acaaaccgtt tgaaaacggc ttgaatgctc ctgaaacaga agtatatatg catgaaatgc
    12001 caggaggaca gtattcgaat ctacagcaac aggcaaaagc tgtcggatta ggtcatcgct
    12061 gggatgaaat caaacaaatg tatcatactg tcaacttgat gtttggcgat atcgttaaag
    12121 taactccatc atctaaagta gttggagata tggcactatt tatggtccaa aatgatctta
    12181 cagaagaaga tatttatgaa aaaggcgaaa cactaagttt ccctgaatct gttgtgactt
    12241 tcttccaagg agaactaggg caacccgttg gcggatttcc taaaaaattg caaaagatta
    12301 ttcttaaagg cagacctgca ttgaatgaac gtccaggtct tttggctgaa tcagtcgact
    12361 tcaacgaagt aaaaaaagaa ttggcagaaa aaattggcta tgaaccaaaa caagacgaag
    12421 tattaagcta tttgatgtat ccgcaagtct ttttggacta tcaaaaagca tataatcaat
    12481 ttggcgatgt tacattactg gacacaccaa catttttcca aggtatacgt ttgggtgaaa
    12541 caatcaatgt acaaatcgaa cgcggaaaaa cgcttatcat tcgtttagat gaaatcggag
    12601 aacccgatat cgaagggaat cgcgtactgt tcttcaattt gaatggacaa agacgtgaaa
    12661 tcactatcaa agataattcg atcatcagta cggtccaaac ccgattgaaa gctgaaccga
    12721 caaaccgaga acaaattgga gcaacgatgt ctggttctgt tttggatgta ttagtgaaaa
    12781 aaggcgataa cgttaaaaaa ggtgatacgt tgatgattac tgaagcgatg aaaatggaaa
    12841 cggccatcga agcacgtttt gacggagaag tcgcacatgt atatgtgtct tcaggagaca
    12901 ctatttcgtc tggtgacctt ttgattgaag tgactgagaa ataataagcg aaaggtgggg
    12961 aaacatgaaa agactgggtt tgttttggct gattctgcta ttagttattg tggcattcta
    13021 catggaacca gtcttgtttc cgccaaaact tgaagtgaag gaaaactctg atcaacaaat
    13081 atcaaaccaa atgacaacgt ggaaatacca ggagcttaaa gcaactggtt tttctactta
    13141 tatcggaaaa ccgatcgatc agctggaaaa ggattttggc caagcctatg atcgattaac
    13201 tagcggattt ggttttgaaa caagatatta tgaaaataaa gctagtcatc tcacatttga
    13261 agcgaacatc caagacagca gagtcacaac tgttaaagta ctgcaaacag ggcctgctga
    13321 tatcgcgccg ttttatttag ggatgacgat gcaagatctt acgaaaatca ctactatcta
    13381 tactaatttc acttttgagt accatgatac agaagttgga atcgaactaa tggaagagga
    13441 tatgaattat cgtccgctaa ttgcttttga taacgacact tttgctattt tattttttga
    13501 tcaaacaaca aacggcctat tttcagttgt gtatttagat aaagatagtc ttttgaaact
    13561 attgccatat caagtgtttg gtgaaggcct tccacattat caagcagata aatcggctga
    13621 ttgggaatca atcaatcgcg ttaaagaacg aaaaagtacg actttattaa atatgcttag
    13681 aagagaagac gaacttccca tatacaatca aacacttgcc tttcaaaacc acagtcagct
    13741 gctactacat gattttttga caaggcctga ggaaatattg tcagaggaac gtatggctga
    13801 ttggcaaaaa tcgctaggca gtgctcaaac gacagtgaaa ttctcactga cgaatgatga
    13861 attggagaag ttaatcagta aacaaaaatt agaaaatact agtgggctat ttattcatcc
    13921 ggcgattgac gctacttttt cgtttttata ttggtacagc gatccttaca accatgaccg
    13981 tttcatggac ccgggaacgg attctgtagg cattgctttt tctaaagaaa atatgttagt
    14041 cttattgcaa gaagaaaacg gagaaagtag tgatagcagt gatcgttaac gaagagctgt
    14101 ttcaactgga ggatcaatgc ctccaagtcg ctgaaatgat aaaaaagagt aaaacgatgg
    14161 cttcttatct tgaacataaa cagtcaatgg aagaatctgc aaaagtttgc cagttgaaag
    14221 aagcgttctt gtctaagaaa gaagcatttg aaaatgtagc ggcatatggt gagtatgcac
    14281 ctgattatcg tgaaaaaaaa cgagctgttc gaaaagcaaa aagacagctt gatatggatg
    14341 agcatgttgc agctttccga tatagtgaaa cacagctgca aagtctgttg gatcaaattg
    14401 tccaagtact tgccgaagaa gtttctaatg aaatcaaagt agatgcaggt agcccgtttt
    14461 ttgaaacgaa aaaacacttt ggatgcggag gtaattgtca tgcaattaga caatagtgaa
    14521 tttgaaataa caaaacgacg cgggctgatc gtatgggtct acagcttaaa acaattaaag
    14581 aatctgaaaa agttcggctt gttgcattat gtctcaagaa gaatgaaata tgtcgtgctt
    14641 tacttaaatg aagagacatt ggaagaaaca gaagaacgaa tccgtaaatt acattttgtt
    14701 cgaaacgtag aacgttctta ccgaccggat atcgaaatga attttgccga aaaaatagga
    14761 aacaaaacag tattaaaaga cgaaggtttc gaaattgaag agctaaatac agaaatccga
    14821 ttagctgaac atctttaaaa atctcttatg agcagttttt aaataaaaaa taacttggat
    14881 aacgatcgat ttttgatcat tgtccaagta tttttttggt atactagtac cagtaccatt
    14941 tctaaggagt gagtagatgc ggataatatc aggaaattat ggcgggcgcc gtttaaaaag
    15001 tttagcaggg gcgaacacca gaccaactac agataaagta aaagaatcca tattcaatat
    15061 gattggacca tattttgatg gagagacagt attggatctg tttgcaggaa gcggtggttt
    15121 agctatcgaa gcagtatcta gaggctgcag tcatgcggta tgcgtagaca aaaattacca
    15181 agcgctaaaa atcatcaaag aaaacatcga aattacgaaa gaaccagaga aatttaccac
    15241 aataaaactt gatgcggaca aagcaatcga acggctagca gcggagaaac aacaattcga
    15301 ctacttgttt cttgatccgc catatgcaaa acaaaaaata atcgatcaaa tccataaaat
    15361 ggaagaactg ggactttttt ctaaaaccgc agttatcgta tgcgaaacgg ataaaacagt
    15421 cgaattgcca gaaactgtgg ctggattcca tcaaataaga aaacagattt atggcataac
    15481 ggccgtaact atctatagaa aagagggaaa tcaatgaaac gtgcattatt cccaggaagt
    15541 tttgatcctt ttacaaaagg tcacttagat acggtggaac gtgcggctaa attattcgat
    15601 gaggtagtaa ttggtgtttt tatcaatact tcaaagaaaa gtttgtttcc cccagaagaa
    15661 agaatgacat tgatcacaaa agctgtttct catcttccta atgtaaaagt catgcatcaa
    15721 gaaaatcaat tgacggtaga aacagcaaaa gagattggtg cagatgcgtt gatcagaggc
    15781 atacgaagta tcaaagactt tgaatatgaa cgagagattg ctcagatgaa tcatcatcta
    15841 tatgatgaat tggaaactgt cttcttatta gcaaaaccag aatatagtca cgtgagttcc
    15901 agtattttaa aagaagtact gcactttggc ggagatgtga gcagctactt accgccagtg
    15961 atcaacgaag ctttagctgg aaagcgtgaa tcaaatgaaa gctaaacaat ggttgaaaac
    16021 agtactgatt atcatatttg gaatgttgat cattggattt gtagtagttc ccttgccgta
    16081 ttatgtagaa tcaccgggag ccacgatcga tctgaaagaa ctaattacgg tcaatgataa
    16141 ggaagataaa ggttctgggt ctttctctct tacctctgtt ggtatcaggc aagctacagg
    16201 tttcttagct ttaaaagcta aaatgacccc ttttgaagaa ttgatcagtg aagaagaatt
    16261 gactggtggg gcgacaaatg aagaatataa tcaaatgcaa cagtattata tggattcttc
    16321 ccaaaatgca gcaatcgaac aagcgttaaa acttgcagac aagccttata aaatggaatt
    16381 caaaggcgta tatgtcatga gtgtagagaa aacatcgaat ttctttggca agctttcagt
    16441 tggagataca gtaacaaaag tagacgggaa aagctttcaa agtacagaag catttatgga
    16501 ttatgtaaaa agccaaaaag ttggacaaac aatcgaaatc agttatttgc ataatggtga
    16561 agaaaaaaca gcttctggtg atctgatcga gttaccaaca gataaaaagg caggaatagg
    16621 tatcacgctt actgaccata ccgagatcga atcagataca gatgttcgta ttaattctgg
    16681 ctctatcggt gggccatcag cgggattgat gttcacttta gaaatctatg aacaagtcac
    16741 tggtaaaaac ttgcgacatg gtaagcaaat tgcaggaact ggaacgatca acagtgaagg
    16801 agaagttggc cgaatcggag gaatcgataa aaaagtggca agtgcggata aagctggcgt
    16861 agagattttc tttgcaccag atgatacgat ttcagacgat gttaagaaag aatatcctga
    16921 aataaaaacc aactacgaag aagcaaaagc tgccgctaaa aaattgaatt cctcaatgaa
    16981 aatcgtacca gtaaaaacag tacaagaagc gcttgactat ctagagaaaa tgaaatgaaa
    17041 ctaaataatt aaagaggggt tgtgacacga gttctgtcac aacccctctt tttcgtcaaa
    17101 aatcttcacg ataaaaataa aaacaaaaga aaaaattgac gtaattagat gttgaaggga
    17161 cgattttgcc agagctcact gtttcttcaa ttttaaatga aaggagtaat gaatgatttt
    17221 tactggtcat tctttgaaaa aagaacaatg atagaaaaaa tcagcaagaa aatactagct
    17281 tcacccaaat tgatcatttg gatgtctatg atagttctta tcttttgtat cgcaagtggt
    17341 tttttattat caaatttgct attttataga gaaacagaag agactgctgc aaaagaaata
    17401 ctttcttcgt ttagttccag tggagaagat ccatcagctg aaggaaacga tgtagaagag
    17461 gatgctcgtc caaaggtcat gtacaccgat atcaaaggaa gtgtgaagga gcctggtatc
    17521 tattcctttt cttcagaaga acgggtctat gatgtactaa agcgtgcagg tggattatta
    17581 gaagaagcgg atagcgatcg aatcaacttt tctgcgaaga tagaagatca gcaagtccta
    17641 tatatccctg ctgtaggtga agagctgcca gaacatcttg atcagttggc atcctcggaa
    17701 ggaaaacagc ctacagcaga ttccgagcct cccaaaataa atatcaatac agccactcca
    17761 tcagaacttc agcagattcc tgggatcgga tcagtcaaag cgcaagaaat cattcgattt
    17821 agagaagaaa acggatcctt ccagaaagtg gaagacctgc aggagatttc tggtatcggt
    17881 gaaaagacag ttgaaaaact aaaaaacttc gttacaatta aatagaatga ttcaaaatcg
    17941 actggcacga gcaataaatg gacgctaatg gatactaaga ataatccaat tctaagagct
    18001 tggcaggacg aggaggtagt atttttggat aggaaatagc ttgattgttc gcaaagaaac
    18061 attaaaaaaa ccaatgctga atgtctatct ccaaaataaa atatctggga ttcatattat
    18121 gaatacggct gtcagcggca ataacagtca agctctacga gaacgctttg ccaaagatgt
    18181 acttagctat acagcagata aagtatttat tctcattggg acgaatgatc tagcagagca
    18241 taaacaactt tctaaagaaa cgtaccaaaa aatctgcagt ggatgatagt tcagctgaaa
    18301 aaagactata gttctgatca tgctttcttt atcacgccac cagcagtcga tgagacaaaa
    18361 caaaaatatc ggaataacaa gttgatcaaa gaatatatct ctgttctcac agaagtagca
    18421 aaacgccatg attgtgaagt atcgattttt atacggaatt tcttgaacaa atggattcga
    18481 agaattttta taatgggcta atggatgacg gacttcattt cggcagtgaa gggtaggatc
    18541 ttttgtcaga caggatgatt cgttatattt aaagaatatt ttgtttaagc aatgagtact
    18601 ttttaatcaa cttgaaaaaa cggaggaata taaaacatgt tagaaaaatc ttcaaaagca
    18661 tgcgatacga ataaagaaaa agttactgat aagtcacata atgaacgcat tccttgggac
    18721 cagtacttta tggctcaggc agtactgtta tctttacgta gtacgtgtac acgtttagaa
    18781 gtaggagcga cactcgttcg agaaaaaagg atcattgctg gcggctataa tggagcagtc
    18841 agtggagatg tccactgcat agatgaaggg tgttacgtcg tggatggaca ctgcttgcga
    18901 acgatccatg cagagatgaa tgcactccta cagtgtgcaa aacttggtat cccgacggaa
    18961 ggttcagaaa tctatgtcac acattttcca tgtttggcct gtacaaaggc tttgttgcaa
    19021 gctggtgtga aagggatcaa ttatctccat gactatcgaa atgatcctta tgcccaagcg
    19081 ttgatcgaac aattaggtgt atctgtacac caagttgctt tagactcaca gcatttttca
    19141 aaacttcaaa atgaattaac aggaaaacat gtctagcgtt ttagaaaggc atgttcgaaa
    19201 tcggttgatt tttccggcaa ttacgtcgat gattatctgt ttgttgtatt acactgacaa
    19261 ttggggttat cgtttgcttc ttgccggaat ttttttatgg ttttcgctaa aagaaggacg
    19321 ttttatcgcg tacttgtgta ttttgtgtag cataacggta tccatttctt ataggctaca
    19381 agagtattca tttttagagg aaaaaacaac atctgcgcaa attactgtat ttacagatac
    19441 gattcgtgca aatggtaatt ttttgacaat ggaaggaagg ttatcagaga ataaacaaaa
    19501 gctccagctt tcttatcaag taaacacaga agcagaattg aaaacatggc ttaacttccg
    19561 aggaaaggga atagaattga ctgctgaagg cgagttcatc tctccagcaa aaaagcggaa
    19621 tctttataat tttgaccaag aagcttactt caggaaccat agaatttctg gacggttcca
    19681 aattcaaaag attgaacaga tcaccatgca aaatcactgg cgaaactggc ttcagagaaa
    19741 aagaggatct tttatctcct ttgtccaatc tgaatttccg caaaaaacaa gtgtatatat
    19801 taattcttta ttatggggct ataaagatgc aggattcaga gaaccagaag aaatttttag
    19861 ggagagtggt ttgctgcatc tgttcagtct atcgggtcta cacatccaat tttatttagg
    19921 gtggctgtat tatttgtttc gacgtatcgg atggccacta cacgtcagta ttattccgtt
    19981 gagtctacta actatctgtt atgtttctat tgcaggaagt tctatttctg ttttgcgtgc
    20041 tagtttgctg tttttattgc gattgctagt caaattgctg aataagcatt attccaccat
    20101 ggacttattt gcagttgtct tttggcttct tttactattt gatcctcgat ttctttttca
    20161 tgcgggtgga cagcttagtt ttttcatgag ttttttgttt atcctgattt cttttgatgg
    20221 agattggttg aaaaaaacgg cctctcacgt cctgtttact atgatcacga tgccgttgat
    20281 tgtatggcat ttttatgagt ggcctatttt aggtagtctt tttacattac tgattgcacc
    20341 ggtattcaag tttctttttc ttcctggtgt gttatttctc acactcttca atcgctggct
    20401 gccggaaact ttacttttgc tgatagaaga gggattaatc ttatttgagc gaataatcga
    20461 tttttcttca gggtcgtctc tcattatagg acgtttgccc cttgtgctat ctgggatgta
    20521 tattttcgga ttactgattt gtatggatag gttgaaagaa caacctgtga aacacttgtt
    20581 ttatgttggt cttttttctt ttattttttt gactttgcca tttttacgct tttcttcaac
    20641 gatcgctttt atcgatgtcg gtcaaggaga cagtatcttt ttgcaaagtc cttttcgaaa
    20701 agaaacgatt ttgatcgata ctggaggtca gttacacttt gaaagagaaa agtgggcgca
    20761 aggtatgata cgtcctcctg ctgaaagaaa tatcgttcca tatctaaaag gtcaggggat
    20821 aagtgtaatc gataaactca tcttgacaca cgacgatacg gatcatgtgg gagaattacc
    20881 cacgttatct cagcatttta ctatcaaaaa aatttatatc ggttggggcg ctggacgaaa
    20941 tgagccatta caaaggcatt tatctgaagc tcaaaaaaat ggtactgaga tcattgaaat
    21001 caaacatggg gacaggatca gcggttatta tgatctttat gtattgactc cttttgaaaa
    21061 gggagaggga agaaatgaag attcgattgg tctatggatg gaatataaca ataggcgctt
    21121 tttattttta ggagatttga atcaagcaat ggaaaaacaa ttgcttttga tttatcctaa
    21181 tctgaaagcc gatgtagtca aattaggtca tcacggtagc cgtacgtcaa gtaacacaga
    21241 ttttctttcc catattgaag caaaacatgg gattatatcc tgcggcgtaa ataatcgtta
    21301 tggtcacccg cacagcgaag tattggagac actagaagaa aatcagataa aaacatggcg
    21361 aacggatcaa caaggaatga tcacctatcg ctggcatcca gtttttcatc ctcatgggct
    21421 agtggaaaca atgatagact agtaattctc atctgctcat ggtaggataa tttttgaaag
    21481 tggtgagtaa tttgagtata caagcagagt tacaaaaaat acgtaaagaa aaacttgcat
    21541 cttgttactt gattttagga acagaaaagt ttctccaaga tcaagttcgg acagaaattg
    21601 tcaaaaaaat ccaattagag ggcgaagatg acttaaattt tttaagtttt gacatggaaa
    21661 atgcaagttt agatgaggta gtagctgaag cagagacgtt gccgtttttc ggagatcaac
    21721 ggttagtatt cgtagagaat ccttattttt taacaggtga aaaagtaaac aatggaatcg
    21781 aacaaaatac tgatttattg acggcttatt tgaaagaacc attagaatcg actgtattag
    21841 tattctttgc gccatatgaa aagttggatg aacggaaaaa agtaacgaaa caattgaaaa
    21901 aaacagcaat tatcatcaat gtccaacagt tgaatgaaaa agaagtacgt caatatctga
    21961 tgaatacctt agagaattcc tcaatcaaaa tggaacgaag tgcaattgat ttattcttga
    22021 ggttgaccga cttagattta tccaaaatga tgagagagtt acagaaattg gtcctttatg
    22081 gacaaaatca acagcaaatc acggtaagag aagtagaaga attagttcct aaaacgttag
    22141 aacataatat ttttgacatg acccaatata ttttaaaagg gaagacggaa caagctttgc
    22201 gtttatacga agatttagtt atgcaagggg aagaaacaat caaaattaat gcgattttgc
    22261 tttctcaatt acgtttattt ttacagacaa aatttcttgt aaagattggt tatcagcagg
    22321 caaatattgc tgaaacattg aaaatccatc catatcgtgt aaaacttgcc atgcaggaag
    22381 taaaaaaatt cgatgaacat ttgctggtac agttgtttga tcaattagtt gagatggatt
    22441 atcaaatcaa aacgggacaa atcgaaaaag aattaagttt tcaactattc gttttgcgca
    22501 cagggcagtt gttaagaaat cgataagaag aaagacgagg gcaaagcatg cgggaaggtc
    22561 gtttgttttt gtcctttttt tgacaaataa aaaaatcggc cgaagccgat tcttttattt
    22621 agcgattttt ttgcttaaac gagatttgtc gcggttagct ttgtttttgt ggattagacc
    22681 ttttgattca gccatatcaa tagctttaac agcttcttta tataacgcat ctacgttgtc
    22741 agcacctgaa gctacagcat cttcaaattt tttgattgca gtacgcatag cgcttgtttg
    22801 agatgaattt ttcacattag cgttttcgct tgtacgaaca cgtttgattg cagattcaat
    22861 gtttggcatt agtttcacct ccgataactt aagttcttac gcgtgttacc gcataattca
    22921 acattcatta ttatacctaa aggcagcgtg ctttgcaata tgaacatgac aagttttttt
    22981 ccttaacagt cttttattag gatttttttt cagtaaaaac ggatagtaca agctgaaaaa
    23041 aatcaaaaag acgctattgt gggaggcaat agcgtcaaac caaactagat ttttatttat
    23101 ctatttaaac aaagtttgaa tgggaatgtt tggtactaag ttattatata acaaagagaa
    23161 ccgtttgcaa agtttttttt gaaaagggat acgattccct gttgttcttt gtttttctat
    23221 taagaaactg cagttatttt ataaccagta tcctttgatc agtaaaggct attctggtgt
    23281 accgattatt ttaatgtatt catcgatatt ttttatcaac atttttttta tgaccaactg
    23341 gttccgacgg ttccatttgc cacttacttc cagcacatat ttatttaggg gcaaacgtaa
    23401 gatatatttt gccaaatagc gatctttaag caggccattg atcacacggt cctcaagctg
    23461 caagttgatc ccagtcagca tcattggctg ttcttgtaaa acgtacgtat caattaaaag
    23521 accttcaaaa gtttccacaa gcgtcttcct ttcttttcaa agtaacctga aaacaggcac
    23581 taattcttcc attttgatat tcgttgaagt ggatgcgatc attctttctt ttgtccactg
    23641 taatctagta taacaaaaaa gagcagaaga gccaaaaaga aaaaagagca tcgaattcta
    23701 taggaagtct cattggcacg gcctaaatta ttacaaaaca ttgattaaaa aaagcccctg
    23761 catcgcagga gcatccccta tatcgtttta ataggtcaat caaaaagcag tatcaaacac
    23821 attttgtatc aaatcaggaa ataatagata agctaagccc agaatcaaaa gtaccgctag
    23881 aagaaaccag ccgattgttc ctatgaaata ctttaatttt tcgaacattg tatcacctcg
    23941 ttgtcagtat atcacggatc agtaaattgg gggtgttaag aatctcctaa aatagacaaa
    24001 atttgttcta ttttcctaaa gcaaatactt gtaagataca gtaagtgata ccagaagcaa
    24061 tgaccggtcc ggcggcgatt cctcttaaga agacaacacc taaaatcgtg ccgaaaacaa
    24121 gtgcaacagt gatttgtgga tcagaagcaa tcaatcctac acccttggaa gataagacag
    24181 ccactagccc accgcaaaaa acagctaccc agccaatagg ggatttaaag gcttctaata
    24241 gatcacgtag accgatttct cctgttgcga tagggattag aatagccgct gtgatgatcg
    24301 tcaccccaat attgattcct ttgccttgaa gccaagggaa aaaggaacgg gaggctggta
    24361 tgagcttcat aatcaaaaca aaagctgccg ctatcatcag actttgattt ttagctaaaa
    24421 ttgctacaag taaaataatt cctaaaaaaa gccaactttc catgaaatat attcctcact
    24481 ttcttcgaaa caatcataac atgtttttag aaagttacaa aagtaggaat tcatccttca
    24541 agacctattc ataagctgtt tttttcataa aataaacaaa atcataaaaa aacactttgc
    24601 ttatttgaca gaagacagcc gcgctttgta tagtggacaa gacttacatt tttttgaaga
    24661 atcagaaaga tcaggtaaag gtcttagtaa acgcaagcca ttcagaataa ccaatattgt
    24721 gcttccttcg tgtcctacaa ctccaagagg tagatttaaa aattgcagga agttagaagc
    24781 aatcaacaag agaataacaa gaatcgaaaa aataatattt tgtttgatga tccgccgcat
    24841 ttttaaagat agccgatgac tgtagacaaa tttatttaag tcgcttttca ttaagacgat
    24901 gtctgcgata tccatagcgg catctgtccc ttgccccata gcgacaccta aagaagcggc
    24961 tgctaaagca ggcgcatcat tgactccatc accaaccatc gcatttactt ggtaaagttc
    25021 attctctttt ttgactagtt gtgttttttc ttccggagta cagcttgcat agtaatgttc
    25081 aatccctact tgttcagcaa tggcctttgc tgtgccagaa tggtcgccgg ttatcatact
    25141 tgtatggatg ttttgggatt taaagtagga aattacttgt tgggtatttg cttttggaat
    25201 atctagcaga cctaaaacag caattaactg ctgatcttta gaaagataaa tcacggtttt
    25261 gccttgattt tccaaacgtt cgatcatttc cgcaatctct ttgctgatta tcatcgtctc
    25321 aggatcatat gcgtgcttgc caactttcca ttgcgcacct aaataggtag tttgtaatcc
    25381 aaaaccagta atttcttcta ttggtaagta ttggatttct tcaggtattg tgcaatcgaa
    25441 atattgcaaa atcgcatgag caagtggatg cgtcgttttt tgttccatag caacaagaag
    25501 ttgttgtgcc aaatcctttt catcaaagaa aaagctgtca gttacaactg gttttccttt
    25561 agtgagcgtt cctgttttat caaaggcaat cgcttttaat tcagaaagct tttctaaatg
    25621 gataccgcct ttgattaaaa tcccattttt tgcagaattt gaaagagcag ctagagaagc
    25681 tggtgtagca gatgcgacaa gcgcacaagg agaagccaca actaataata ccattcctcg
    25741 ataaaagctt tcatcccaac tccaacctag gaaaaagtgg gggagaagga tcattagagg
    25801 aacagcaagc aaaatcaatt ttacataatt attttcgatt cgttcgataa atgaagctgt
    25861 ttgagttggc gtattctgcg cttcttctac taattggata atcttagcaa agacagtttc
    25921 ggaactcgtt tttgttactc gaatcgtgat gggatttcct tgattcagcg ttcctgcata
    25981 catctcatcg cctaaatatt tttctacagg cacagattca ccgcttaatg cagactcatc
    26041 gatcgttgcc tgtgtgcttt ctaaaaagcc atctatcgga acagctgctc ctttagcgac
    26101 aaataccaag ttatccacct ttagttgaga aacatctact tcttgcattt gtccattttc
    26161 ttttaataat tgggcttttt gtggttgaag attcatcaaa gcagtaattt ctttttgact
    26221 tttattagtt gtgtattcct ctaatgcacc gcttaaacaa aagatgaatg tcaacattgc
    26281 tccttcaaag taattaccga tcaaacaggc accaatagca gctaaagcca tcaataaatc
    26341 cacattcagt gtccgatcat tccacaattc tttcaagcct tccgatgttt gtttatatcc
    26401 accgatgata atggcactga tataaaaaag tggggtgatg gtaagagaga atcggtcgaa
    26461 gataaaaccg aagatcataa ataaaaggca gccgattgtt gctgcaaaag cttttttctg
    26521 attagtcatc gttattagac ctccttgtca aaacaaggat agcataataa aaatgtaatt
    26581 gataataata acagctaaat gagaacgata atggttatca tttggtgatt aagcttgatg
    26641 ttgtttttat tatttataat gattattatt aaagaaaata aaaaaccaga acatttctca
    26701 aataagagaa gtgttctggt tttttctaca ttgcaataaa ccaataacca atcaaaatga
    26761 taccaaggat aatacgatac cagccaaaag cggtaaagtc gtttttcttc aaatagctta
    26821 gtaagaattt gatagctaag acggaaacca caaaagatac gaatgttcct acaagaagaa
    26881 caacagtttc ttccattttg aatgaattcc cttcagctaa atatttgacg atcttcaagc
    26941 cgcttgcgcc aaacatgaca ggaatcccta agaaaaatga aaactctgta gcgacaaaac
    27001 gcgaagcacc aatcaagata gatcctagga tcgttgcgcc agaacgggat gttccgggaa
    27061 caagtgccaa gacttggaat aacccaacaa gaagagctgc ttggtacgta aagtcattca
    27121 gagaagtcca acgaggtgtt ttgtttttgt tgcgtttttc gacaataata aaggcaatac
    27181 cgtaaacgat caaaacaatg gcaactggta agaacttgtg gaaatttgct tctaaccaat
    27241 catccaaagg caaaccaatg atgatggctg gtatgcaagc aacgatcact ttaaaccaaa
    27301 gtgtccacgt atcttttttt tctaccgttg atttacttgg agaaaatggg tttaacttgt
    27361 gaaaataaag aacgacgaca gccaagatcg cccctaattg gataacgaca ttgaacatcg
    27421 acataaattg tgaactggca tccagtttga tgaattcatc tgctaggatc aaatgaccag
    27481 tactactgat gggcagccat tccgttattc cttcaataat accgagaatc actgctttga
    27541 taatatttgc aaaaaacata aattccttcc ttcccaatta agatacatct tatagtatac
    27601 cgttattttt tgagaaaccc aatctgtttt ttaatattaa gaaaaacttg agaaataaag
    27661 cagataagta cacgaagaga aaaaagaaga caaagccctc gttcagactt tgccttttat
    27721 gattctctgg taaaaattaa aatatcccca ttatagaaac gagtcgcgac tatgtgtttt
    27781 cccgttatcg atataagacg tatcattgat gccgtggata gaaaatcgac cgtttgcata
    27841 agtgaccttt gttacgctgc catttaaaag cccagcacga acaggctgtt taggatcgat
    27901 cagattaagc aaaaaggcga tcgtcaaacc atgagatacg actaaaacat ttccaccacc
    27961 atttttttcg cagtgatggg cgatatcttc aaatcctgtc catacacggt ctcgcaatgt
    28021 ttcataagtt tccgcccacc cagcggtatc taagttgtat acagcattag caatttgttc
    28081 aaaagaaact tctgtggtaa acatttcatc atatgtttta aaattcaata cacgagggat
    28141 cacaccccac atttccatgt catatccacc ttcaagagac ccgaaacacc attctctaat
    28201 ccttttttca ataaaataag gaatttcttt tcccttagaa tgttctccta gaataatacg
    28261 ggcagtttcg attgcacgac cactgtcact agaataagca aatgaaaagt cgacattttt
    28321 taacccgcga cctaaataat gaatgccttc ttgtccttgc tttgtgaggg gtgtatcaca
    28381 ccagccttgt actcgttcaa ttgtattgaa catcgttttt ccgtgtcgta cgatataaag
    28441 tgtagtttct gtcattttat tctcctccta agtcgtttta acgattgaaa aaaggatttg
    28501 tccttttttc ctgttcaatc gtcgttgcat cgccatgtcc tggatagact gcaaattcat
    28561 caggtaatac aaacagctgg gtccgaatac tatgaagtaa ttgttctaaa ttccctgtat
    28621 acagatcggt tctaccgata cttcctctaa acagggcgtc gccagaaacg acaaaatcat
    28681 caaaaataaa gctgacgctt cctatagaat gccctggggt agggacgacg cgaaaagaca
    28741 tgtttcctaa agtataagtt ttcatttcaa attcatattc tgcaggcgag acaataacat
    28801 cagcaatatc atcatgtcta ccgagtccag aaagattcaa aatcggatca cctagccatt
    28861 tttgctctag tgggctgaca taaactggga tttgataata cgcgcgtaaa ggttccactg
    28921 caccgatatg atcgtagtga gtatgggtaa gcaaaatcgc taccggctgt tggtttgttt
    28981 ttttgatttc ttgtttgatt cgttccgcat cttctccagg gtcaatgatc aaaagtgctt
    29041 cttcgttgta gaccaaataa cagttttctt caatcattcc tgtttttaat ctttcgatat
    29101 gcatgtgcat tcctccttga tagccaaaac ttctttcatc cattagtata acgcattcat
    29161 tgctaggaaa caaaaacagg tacttatttc tctttactta tctgaattat cagactatat
    29221 gaaaagtatt tggatattct aaagagaagg aaagtaagtt ttcatagcat tttaatatgt
    29281 ccttgttaca ttagattcat ccaaaaatcc aaattctata tcctatatcc ctgcctaatc
    29341 agcagggtgt ttttttatgt ttggaaagag agctcttttt gctaaactag gttttttcag
    29401 gtaatcttag agagaaagag gtggtggaaa tgatcaaaca gtcaaaaata tctgtgcggc
    29461 acttagtaga atttattttg cgacgtggaa gtatcgacaa tcgtaaaaaa agtaaccata
    29521 ctgcattaga aggagcgaga atccaccgca agctccaaaa agatgcggga gaaaactatg
    29581 aaaaagaagt ctttttaaaa acaacagttc aattagatga atattacctg atcgttgaag
    29641 gccgtgcaga cggtattttc aaaagagacg gtatttatta tatagatgaa atcaaaacct
    29701 ccgaaccacg atttgaagat ttagaaccag aacaagtgga acttttcttt catcaagcaa
    29761 gggtgtatgc ctatatctat agtcaagaac atgatttaca agaaataaat ctgcaattga
    29821 cttacttcca gacggcagaa gaagtgatca cgagaaaggt tgaacatcag acaagagagc
    29881 agttagaaac tttttttaaa aagctgactg aagattataa agaatggctt gtttttcaag
    29941 aaaactggcg cactgtacgc aatgcctctt tgatggcttt gaaattcccc catgatgagt
    30001 ataggaaagg tcagcgtgaa ctagcggtag ctgcttataa aacgatccgt acgaaacaga
    30061 aattgtttgt agaagcacct accggaacag gaaaaacaat ctctacgtta ttccccgctt
    30121 tgaaagctgt aggggaagaa gagggagaaa agatctttta tctaacggct aaaacaataa
    30181 ctagacaagt agctgaagat gctatgacag cattaaaaga tacaggagct gaagtaaaaa
    30241 gtgtcacttt gacggcgaaa gacaagatct gctttttaga tgagacgacc tgtaatccag
    30301 atcagtgccc atatgccaat ggttattaca atcggattaa tgaaggttta tgggatcttt
    30361 tgaatcatga gaatcagatc actcgtgaag tcatcgaaac ttatgcaaga aagcatacac
    30421 tttgtccttt cgaactatca ttagatgtca gtatctggtg tgacgtaatc attggggatt
    30481 acaattattt atttgatcca actgtttatt taagacgttt ttttgaagat gaaaaaaacg
    30541 aggactatct gtttttgatt gacgaagcac ataatttggt gaatcgttcc cgagagatgt
    30601 attcggcaga actatcttat gaaaaaacga aacgaagtaa ggaagctata ccaaaggaat
    30661 tcaaaaaact tcatcgccgt ttcaacaaac tattaaaaga atttgattcg atccgagaaa
    30721 tagcaaaaga agatcattgg gattatcatc atcaaaaagc accggctgaa tctttagtaa
    30781 aggcaggtta tcaattaagc gaaaaaatta aagaatggct ggcagaattt cctgagcacc
    30841 catcacaaga acaattgctt ccttattatt ttgatttgct tcattttttg aaggtcagtg
    30901 aattttatga tgatcactac gaaacaacag tcgaaaaaac gtatcgcgat ctgatagtaa
    30961 aagagttttg tattgatcca agcttgtttc ttgagcagtc gttggataaa ggaaaaagca
    31021 gtattctttt ttctgctagt ttttcaccac tctcttatta tcaggaaaca ctggggggac
    31081 aaaaaagtct ggcttacaaa ctgccgagtc ctttcccaga aggaaaccag caagttttca
    31141 tcgcaaatta cctagagacg acttatcgaa aaagggaaca cagtctggca aaattagtag
    31201 agacgattca tgcttttgct gatggaaagg tcgggaatta tttcgttttc tttccttctt
    31261 atcaatattt agatcttgcg gtggaagcat tcagacagca atatcccgac agcaatgtgc
    31321 taatccaaga aacggacatg aatgaggaag aacgcgaacg atttttaaca aaatttcaaa
    31381 caaatcccgc tgaaacattg attggatttt gcgtacttgg cgggattttt tcagaaggaa
    31441 tcgatttgag aggggaccga ttgatcggca gtatgatcgt aggtgtaggt ttgccgcaga
    31501 tgaatcatga gcaggaactg atcaaatcat attatgatga gaaggaacac cttggatttg
    31561 cttacgctta tcagcttcct ggaatgaaca aagtcttgca agctggtggt cgagtgattc
    31621 gtggcatggc ggatcaagga atcgttgtat tggcagatca acgattttct agtttttctt
    31681 atcgacgttt attcccacaa cattggcaaa cagcaagaga agtacgaagt gttggggaat
    31741 tggaagaagg aatacagcgt ttttggttat caaaagaagc agtaaatcaa aaaaacgagg
    31801 aatgacagac gtcagcctcg ttttttgatt aacgatagtt agtgaattgc aactcgattg
    31861 gtaagtcaag tttacgtaat aaagcaatca cagcttgtag atcatcacgg ttcttacctg
    31921 tcactctaat ctgatcttct tggatttgtg cttttacttt cagtttttca tttttgatag
    31981 ctgtagtgat tttcttcgcg ttttcacgat cgattccgtt gaccaaatcg gcagtttgtc
    32041 gagctttgcc acctagagcg tgttctgatt cggaaaaatg gatatttttg attggcacat
    32101 ttcttttgat cagcttacca aataatacat ctttgatctg ttcgattttg aaatcatcat
    32161 cgctcaatac aactagattg ctgttctcta atttgatttc tactgtagaa tccttgaagt
    32221 caaagcgatt ggacaattct tttgttgttt gctgaatcgc attttttact tcttctgtat
    32281 tgacttctga tacaatgtcg aagctagcat cttttgccat aggaaccctc ctgatataca
    32341 tataaatatt taccatacat ctatcttatc ataggattag ggcagattct tacctgtttg
    32401 ctaaaattct tctcatttga gttcgaagta ttttatttta atagataaac tgttattctt
    32461 acatttggaa taccaaagta ggggagaggt gcttgtgagt aaaattatga ctgcgtatca
    32521 taaaaatgaa agattaaaga aaatagtggt tattttgata accggattga ccgctgcggt
    32581 cgctttaaat tattttttga ttccagccaa tgtcttttct gcagggatga atgggatcgc
    32641 tcaaatcatt tcctcgcttt tatatgaatg gtttcatatc aagatagaca caggaacatt
    32701 tatttttctt ttgaatatcc ctgtgtttgt attagggttt ctcaaagtag gaaaacgagc
    32761 aacgatcctt agttttgtga atgttgtatg tgtatcggtg atgacgatgc tgcttccagt
    32821 cggacagatc actgataata ttttgatgaa tgccctagta ggcggtgttt tattaggtct
    32881 tggcgtagga ttatctttaa aaatgggctt tacaactggt gggatggata tcatctctct
    32941 tgtactttca caaacaactg gaaaaacagt cggaaactac atgctgattc taaatggtat
    33001 cattgtagtc atcgcaggct tcttctttaa ctgggaaagt gccttgtata cgattatttc
    33061 catttatgca atgactcaag taattgatgc catccacacg agtcatcaaa aagtaacagc
    33121 aatgatcgtt accgtcaagc cagaagaggt aacagaagaa ctagcaaaac ggatggtacg
    33181 tggaatgaca ttactgccgt ctgtcggtgg ctattcaaaa aaacaaggga acatgatcat
    33241 gatggtcatc acccgctacg aaatgtatga tcttgatcag atcgttcatg aaatcgacga
    33301 cgatgcattt atcaatattt taccaactca ttcagtattt ggccggtttg caaatgaaaa
    33361 cgaacaaaaa atgtatcgaa caactggcgt attgccagaa ataaaaaatc gttcacaaaa
    33421 aaaatgagga atcaggttta ttttagaata aatttaatct attacatcta ataagaacat
    33481 ttttataaat ttaaacgaga atcttacttc taaattctca tttacaagca gctaaaaaaa
    33541 gagtatgata aataaaaatc gtgttaagga gtgtcgtacg atgagcgaag caaaccaaga
    33601 acggtctgga caagaaatag aactaatcaa agaacgtatt cttgctgcct tggaaatggt
    33661 gattgatcct gaattaggaa tcgacatcgt caatttaggc ttgatttatg atatagaatt
    33721 caatcctgaa aatggagaaa cagtcattaa gatgactcta acaactatgg gctgtccatt
    33781 agcagatatt ttaacagaat ctatccatga cgcattaaaa gaagtgccag aagtaacgaa
    33841 agcagaagtc aaactagtct ggtatccagc atggacgaca gataagatga gccgttatgc
    33901 acggattgcg ttggggatac gatagaaatc ttggctgact agctatgaga agcaaatagg
    33961 aaaaacaaaa aagcctttta tcctttctag aagaaaagag ataaaaggtt tctttgttta
    34021 taaaattttg tctgctatat tataagttaa ttaaataaat gtgttctatt gataaaggac
    34081 attcaaatcc ctttcctttt ggaatccttc ttttttacat cagtttgatt ttcgtatagt
    34141 attctttctc atcttcacga taaatcgcag ccccattttt ttgttgtgct ttaagactgc
    34201 caagattttt tttatttaca gacaaaagtg cctcatcaat gccgattttt tttgcctctt
    34261 gtagtactaa tgctaatcct tgtgtggcat atcctttttt acgataatta ggggagatgc
    34321 cgtaaccaat atggccagcg ccgtttctta atgtttcgtt cagtttatgg cgcaaattaa
    34381 aaattcctac atactcatgt tcatcacttt ctaaaataaa gacggttgcg ggaacgtaac
    34441 tctcaggaag atcgattcct aaagacgttt tttcgcattt ttttacataa tctaaaaatt
    34501 cagcaaatgt atagccataa gcattgttga taaatccact ctcatttggt ccaaagctag
    34561 tttgtagctg ataagctttc tttaaatctg cttcccatag tttgatcagt ttcatcgtat
    34621 tcacctcttt aagtaacctt attcatttgt tttctactat agcactattg ttgtaaatag
    34681 ccatgcctat ttttatttat tgtgtcatat cgtattaaga atcatcgaac aatacaaata
    34741 tgaattacaa taaaaaaatt atttgaagtt cgttcattcg ttacttatga tctctagaag
    34801 aaagaaaacg tgaattactt cagcgtctag aattccagaa ggatagtaat caacagaaga
    34861 aataatcaat atccaaatga aagaagatag ctgaatcgag aaaaagagga aaaaagaaat
    34921 gaagaaaaaa ataaaatagt tactaatccc agagtcgttc gtttaacatt gaaatcgatc
    34981 atatggataa aacagatttt ttcaccaacc ctcaatagat gatggagttg ctcggatgaa
    35041 ttatttgtgt agatggcata agggagggcg tacagaaatg aaaaagaaca aaaatatgtt
    35101 cttgacaaaa aacacatgta atcctaaact aaaagatgtg atgcggctat ggcggaattg
    35161 gcagacgcgc tagcttcagg cgctagtgag ggtgacctcg tggaagttcg agtcttctta
    35221 gccgcataat ttgttttttt ataaagagaa ggtacacctg gagcgatgag aaagaaaata
    35281 acatattatc taaaagaata taggaaaaat tttaaacaac ggaaccagat tcaaataaat
    35341 caaaaaaaat ttgtgtgaag gtgggacagt ttaaaaccta atagattgat tcaaatagta
    35401 gatttataga ttctgataca agaaatactg gcaaacgata gttaaggaat aaagactaaa
    35461 cttaaactca aaaagaggtc atgcgaaaga ataaatcgca tgacctctta ctatattttg
    35521 aatgtgttta aacgttcaaa
//
LOCUS       DBNICI010000008        34952 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.008, whole genome shotgun sequence.
ACCESSION   DBNICI010000008 DBNICI010000000
VERSION     DBNICI010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 34952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.008"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            153..1859
                     /gene="efbA"
                     /locus_tag="AC1ZCC_000348"
     CDS             153..1859
                     /gene="efbA"
                     /locus_tag="AC1ZCC_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HIQ1628634.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2017..2919)
                     /locus_tag="AC1ZCC_000349"
     CDS             complement(2017..2919)
                     /locus_tag="AC1ZCC_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIQ1628635.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2916..3608)
                     /locus_tag="AC1ZCC_000350"
     CDS             complement(2916..3608)
                     /locus_tag="AC1ZCC_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HIQ1628636.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3651..4292)
                     /locus_tag="AC1ZCC_000351"
     CDS             complement(3651..4292)
                     /locus_tag="AC1ZCC_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HIQ1628637.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4330..4644)
                     /locus_tag="AC1ZCC_000352"
     CDS             complement(4330..4644)
                     /locus_tag="AC1ZCC_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628638.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4632..5261)
                     /gene="pyrE"
                     /locus_tag="AC1ZCC_000353"
     CDS             complement(4632..5261)
                     /gene="pyrE"
                     /locus_tag="AC1ZCC_000353"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIQ1628639.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5268..5984)
                     /gene="pyrF"
                     /locus_tag="AC1ZCC_000354"
     CDS             complement(5268..5984)
                     /gene="pyrF"
                     /locus_tag="AC1ZCC_000354"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIQ1628640.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5981..6913)
                     /locus_tag="AC1ZCC_000355"
     CDS             complement(5981..6913)
                     /locus_tag="AC1ZCC_000355"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HIQ1628641.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6910..7686)
                     /locus_tag="AC1ZCC_000356"
     CDS             complement(6910..7686)
                     /locus_tag="AC1ZCC_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HIQ1628642.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7740..10925)
                     /gene="carB"
                     /locus_tag="AC1ZCC_000357"
     CDS             complement(7740..10925)
                     /gene="carB"
                     /locus_tag="AC1ZCC_000357"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIQ1628643.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10930..12006)
                     /locus_tag="AC1ZCC_000358"
     CDS             complement(10930..12006)
                     /locus_tag="AC1ZCC_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HIQ1628644.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12011..13291)
                     /locus_tag="AC1ZCC_000359"
     CDS             complement(12011..13291)
                     /locus_tag="AC1ZCC_000359"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIQ1628645.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13310..14236)
                     /locus_tag="AC1ZCC_000360"
     CDS             complement(13310..14236)
                     /locus_tag="AC1ZCC_000360"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HIQ1628646.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14249..15532)
                     /locus_tag="AC1ZCC_000361"
     CDS             complement(14249..15532)
                     /locus_tag="AC1ZCC_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HIQ1628647.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15546..16085)
                     /gene="pyrR"
                     /locus_tag="AC1ZCC_000362"
     CDS             complement(15546..16085)
                     /gene="pyrR"
                     /locus_tag="AC1ZCC_000362"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HIQ1628648.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16320..17231)
                     /locus_tag="AC1ZCC_000363"
     CDS             complement(16320..17231)
                     /locus_tag="AC1ZCC_000363"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIQ1628649.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17228..17692)
                     /gene="lspA"
                     /locus_tag="AC1ZCC_000364"
     CDS             complement(17228..17692)
                     /gene="lspA"
                     /locus_tag="AC1ZCC_000364"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIQ1628650.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17697..18395)
                     /locus_tag="AC1ZCC_000365"
     CDS             complement(17697..18395)
                     /locus_tag="AC1ZCC_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIQ1628651.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18382..18768)
                     /locus_tag="AC1ZCC_000366"
     CDS             complement(18382..18768)
                     /locus_tag="AC1ZCC_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628652.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18915..20585)
                     /locus_tag="AC1ZCC_000367"
     CDS             complement(18915..20585)
                     /locus_tag="AC1ZCC_000367"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIQ1628653.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20768..21001
                     /locus_tag="AC1ZCC_000368"
     CDS             20768..21001
                     /locus_tag="AC1ZCC_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIQ1628654.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21100..21531)
                     /locus_tag="AC1ZCC_000369"
     CDS             complement(21100..21531)
                     /locus_tag="AC1ZCC_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HIQ1628655.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21610..22023
                     /locus_tag="AC1ZCC_000370"
     CDS             21610..22023
                     /locus_tag="AC1ZCC_000370"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0043137 - DNA replication, removal of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HIQ1628656.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22061..23209)
                     /locus_tag="AC1ZCC_000371"
     CDS             complement(22061..23209)
                     /locus_tag="AC1ZCC_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIQ1628657.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23331..23927)
                     /locus_tag="AC1ZCC_000372"
     CDS             complement(23331..23927)
                     /locus_tag="AC1ZCC_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIQ1628658.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24127..26769)
                     /gene="alaS"
                     /locus_tag="AC1ZCC_000373"
     CDS             complement(24127..26769)
                     /gene="alaS"
                     /locus_tag="AC1ZCC_000373"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIQ1628659.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27157..27588
                     /locus_tag="AC1ZCC_000374"
     CDS             27157..27588
                     /locus_tag="AC1ZCC_000374"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1628660.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27633..28982)
                     /locus_tag="AC1ZCC_000375"
     CDS             complement(27633..28982)
                     /locus_tag="AC1ZCC_000375"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIQ1628661.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29052..30215)
                     /locus_tag="AC1ZCC_000376"
     CDS             complement(29052..30215)
                     /locus_tag="AC1ZCC_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIQ1628662.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30344..30667
                     /locus_tag="AC1ZCC_000377"
     CDS             30344..30667
                     /locus_tag="AC1ZCC_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628663.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30850..32199)
                     /locus_tag="AC1ZCC_000378"
     CDS             complement(30850..32199)
                     /locus_tag="AC1ZCC_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIQ1628664.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32268..33221)
                     /locus_tag="AC1ZCC_000379"
     CDS             complement(32268..33221)
                     /locus_tag="AC1ZCC_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HIQ1628665.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33323..34513)
                     /locus_tag="AC1ZCC_000380"
     CDS             complement(33323..34513)
                     /locus_tag="AC1ZCC_000380"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HIQ1628666.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(34713..>34952)
                     /locus_tag="AC1ZCC_000381"
     CDS             complement(34713..>34952)
                     /locus_tag="AC1ZCC_000381"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628667.1"
                     /translation="KNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYVFL
                     LVYAINKLNIQNTIDFNERASINGCVVFICPGRVK"
BASE COUNT         9948 a         7638 c         6119 g        11247 t
ORIGIN      
        1 taaaaatttg gatttcaacg atagaaacac tgttttttat gtaattagtc caagattttt
       61 ctagactttt gctttactat tcggccaaat tggtttttct tctttttttc gttataatga
      121 atatacttat gaaaagaaat gaggaatgaa ccatgtcctt tgatggtgta tttacccacg
      181 cgatggtcaa cgaattgcgt gaaacgttac tatctggccg gatctcgaaa atccatcagc
      241 cttatgaaaa tgaaatcgtg ctggtgattc gttcacgcgg caaaaaccat cgattattat
      301 tatctgcgca cccaagttat gctcgtgtac agattaccca aatcgattat cagaatcctg
      361 ataatccgcc taacttcgtg atgatgcttc gaaaatattt ggatggcgct attttagaag
      421 acatcgaaca aatcgaaaat gatcgagtga tccatttcca ctttgctaaa cgaaacgagt
      481 tgggtgatct acaaaatatc atattgatcg ttgaattaat gggacgtcat agtacgatcg
      541 tcttagttaa tcgagaaact ggaaaaatac tggatgcgat caaacacatt ggaagctctc
      601 aaaacactta tcgttctttg cttcctggtg tagaatatgt tgctccgcca aaacaagagg
      661 tcttgaaccc atttagctcc gagaaagaaa aaatctttca gcggctttcc caaactgaac
      721 tagatccaaa agcaatccaa cgtcagtttc agggaatcgg tttcgatatc gctcaagagc
      781 tgaccaaaag attactggaa cgtcctaatg aaaagatggt tgtatgggac gaatttttct
      841 cagccatcag ccatcagcct attcctactt tttacgaaac ggaaaataaa gattttttca
      901 caccgattgc ttatcaggta ttgtccgaac aggcatctgc tgtaacagct tatccaacac
      961 tcagtcaatt attagatagc tattatcacg aaaaagcaga gaaagatcgg gcaaaacaac
     1021 aaggcggaga actgattcga aaaatcgaaa atgaactaaa acgaaataaa aataaattga
     1081 aaaaacgtga acaaacactc aaagaatccg aaaacgctga gaattaccgt agaaatggcg
     1141 aactacttac tacttttctg acacaagtac cacgcggcgc aaaagaagtt gttttaccta
     1201 attattatga agaagatcgg ccaatcaaaa tagcactaga tccggcattg acaccaaatc
     1261 aaaatgcaca gaaatatttc catcgatacc aaaagctgaa gaacgctgtt aagctgattg
     1321 gcgaacaaat ccaagaagcc aaagatgaaa tccaatatct tgaatctgtc ttatctcaac
     1381 tggaaattgc cggcccaatg gatatcgaag caattaaaga agaattgact tcagaaggat
     1441 acctgaaaaa gaagaactta aaaaaacaaa aacgtaagaa accatctcag cctgatcaat
     1501 acttttcttc tgacggaacg ctcattcttg ttggtaaaaa caatcttcaa aatgaccagc
     1561 tgacaatgaa gacagctaaa aaaaccgact attggcttca tgcaaaaaat atacccggtt
     1621 cgcacgtcat cattaaaagt gacaagccct cagatgaaac aattacagaa gctgccgagt
     1681 tagctgccta tttttcaaaa taccgatact ctgcccaagt gccggttgat cttgtacagg
     1741 taaagcatat ccgaaaacca aatggcgcca agcctggtta tgttatttat gaaaaccaaa
     1801 agacgattat cgtgacacca gaagaagaaa aaattacagc tatgaaacaa aacaggtaat
     1861 caatgagtgt ttctagaatc atatttgcgt actttcccac aacagcaatt agttataaat
     1921 tgtttgctac aaaaagagga gttgtgacaa aattctgtca caacccctct tttttgatca
     1981 acggtgttca ataaacagcg ttctcatctg cttttcttag ctctgctggt ccagtaaccg
     2041 agtaagctct ttgataaatc tttgagcgat ttcactgagc ggacgtttcg ccgaataaat
     2101 gatataaaat tcatttagtt cgccttctgc caaagggatt aaccgcaagt gctcgcgaat
     2161 ccctccaatc acaataccag atcctgaagc gtaagcatca gtttcaagaa cgatattcat
     2221 caaagtacct cgatcactcg tataaatcac tgtttcttga tcagggatct cgataaggtc
     2281 ttcataaaaa tacgggaaat tatttccatc ctgtgtaaaa cgaacttgag gataagataa
     2341 caattcttct ttggtgatct ctgcgtgttc agctaatgga tgtccttgcc ttaaaaaaat
     2401 atgcgttttg aattttccaa tgctgcggcc acttagctct ccttgttgta aatatcttct
     2461 gataccagct tgattatgct cattcatata aataatgccg atctcactgt gctgctcttt
     2521 gacttcttcg atcactctcg ctgttgtcgt ctccatgatc cgaaattgtt tgatctcgtc
     2581 tttgaattcc tcgagtagct gtgcggtcaa aggacctaag aaatcataat gctgggcagc
     2641 gatcgaaaaa ttgtctctct tttcactcgt tagatagtga gtttctaatg tatcaagctg
     2701 gaccaatatt ttctcggcac gatttaagaa gtcttgccct tcttctgtta aatatgcccc
     2761 tcgattcgtt cgagtaaaca gcgtgatccc taactcggtt tctaattctt tgataccagc
     2821 tgaaagactt ggctgggaga caaacatttt ctttgccgct tctctgaaac taccattgtt
     2881 tgctacagcg accacgtatt tcatctgctg aatattcatt gctcaaactc ctcgaaataa
     2941 taaatttctc ttccattcaa gggctggatt ggtcggttgt tcggtcgagc atactgacca
     3001 tcgaagggga gaagaaactc ttggttcagt tttcctacat gatccattac aaaccattgg
     3061 attggcccgc tggtaggcgg agttgtcaat gagccaatat aatggaaatg gcttttttgc
     3121 tccggaaaga acacatccgg atttacccaa tgctcatgtt tttctaagtt ccaactttcc
     3181 ccattttctt gattatacag ccaaccttcc tgtaccatta gctggtacat gacacccaca
     3241 actagattct cttttttgct gttcatatga acaaaatgaa attcaatatc ttcctgtttt
     3301 ccgttgatga tatgttcaga aggcaagtgg aaatgaatat ctgtcaaata atagcgttct
     3361 ttcttgaact ctacataact gagttggtca aaaggtacta aatgaatcgt gtttttgaat
     3421 tccttgtcag taaaccgttg cttttgataa tgaaaggaaa gcgtgtctcc ttctattttg
     3481 gtttcttccc ccttggtcaa atgaataggc gactgcaatg gaaattccgc acctctcgca
     3541 aaccaatcac ataaactcgc ccaattttcc ggacctttgt ctccagaata gccccattcg
     3601 acatccatat tctttttcaa ctatacttcc ccctgcatct catatcattt ttatttttct
     3661 tctttcttta caatcgttga gacggtttct aaaaacgcct cttttccata aaattcctgg
     3721 ttcaatagtt ccggaaatat ctctttaaag aaatactgaa cagaaggccg atgttcaaag
     3781 cttttaatcg tagtcactgc ctcaatgaac atctccttat aaactttttc cgggttattt
     3841 ttgatgattt catcaaaaga ttcataaaga aggtcaacct tatctgaaat ggctaatagt
     3901 tctccttcta cggtttcatc ttttccttcg aacaaccgac ggcgataaac ttcttggaac
     3961 tcagctggaa tctcttggtt gatgaattcg tccgtcatct tttcttcaac tgtttgcagc
     4021 atccctctta actctttgct tgcgtatttc accggcgttt tgatatcacc aataaaacgc
     4081 tctgtgtagt catgattcaa tgatttttca tagagcagtt tccaatcgat cggttgtcca
     4141 ttcgcttctt caatgtctcc tagaacttgc gcaatagatg ctacacgata cgaatgtgct
     4201 gcaactgtat gctctgtata tttaaaaaat ccaggtgccc ttgtgatcgt ctctaaattg
     4261 ttcaatccta aaataaattc attcaacccc ataaaagtta cctccctttg agatttcttt
     4321 ttccgaatat catatcatta aataactgat attcgcaaca tttccgctct ttaccatttt
     4381 tgaaacagct cccttaccaa aaaatagaga aaatctatat ataatagaaa aaagagaaga
     4441 gattattctc ttctttctgc taaccagtct tagaaaaatg aattttaaaa aaatcaaaaa
     4501 ctgatcacga tagaagtttc ttttcatacc tgatgaaaag atgcttctcg caatcagttt
     4561 ttggattttt gctattccaa ttgatgattc tggagacaca aatcggattt ctctatcttc
     4621 atcaagcgcg cttaaatctg ccaattttca ggatcttttt tccattcttt tagcagagtt
     4681 aattcttgct catcgatata acgatcttct aaagctgctt caatcaatac agaataattg
     4741 gtcaacgtca taagagggat ttcggctttt tcgaaatttg cttttccttt tggcaactca
     4801 tacgtaaaga tagccgcaac accaaggata tccgcacctt ctctttttgc tgcttcagct
     4861 gcttccaaga cacttccgcc tgtagaaatc aaatcctcga tcacaaccat cttctgacct
     4921 tcaaatatac gtccttcgat ttggttacct ttcccatgat ctttcgcctt acttcgaata
     4981 tagaccattg gcaaatccag tatttccgct acccatgcgg catgagggat tcctgctgtt
     5041 gcagttccag caatcacttg tacttcaggg taagcttctt tgattttgct tgctaatcct
     5101 tttgcgatct cttttcggac ttttggatag ctcatcgtga ttcggttatc acagtaaatt
     5161 gggcttttga ttccacttgc ccatgtaaaa ggattacttg gatttaaaaa tactgcttca
     5221 atttctaata agtcttttgc gatactttgt tcaacattca ttttaggtta gctccattct
     5281 gtttgaatcg tttgatatgc tttataagga ttagttgact gagtgattgg tcgacctata
     5341 acgataaaag ttgagccgat cttttttgcc tgagaaggtg tgactactct tgtctgatct
     5401 cctgcgactg ttccttctgg tcttataccc ggagttaagc aaacaaattc gtctcctgcc
     5461 atttctttga tcgcttctac ttcccaagca gaagaaacca caccatctaa tcctgctttt
     5521 ttggcacagc tagcataatg gatcacactt ttttctaaag aaacttcgat tagttgatcg
     5581 gcatgcattt cttcttcact tgtagaagta agctgagtga tagctagaag tagcggacgt
     5641 tttccacctt ctggagtccc ctcttctaat cctcgcattg ccgcctccat catacgaata
     5701 ccgccagcgg catgaacaca ggtgagatca acaccaagct ttgccaatcc ccgcattgct
     5761 ttttcgaccg tattcggaat atcgtgaagt ttcagatcta aaaatacatt atgaccagcc
     5821 tcttttagat aacgtacgat ttcaggtcct tcttggtaga acaattccat tccgattttc
     5881 acaaacaatt tttcttcttt cgggaaaaat cgaagaaagt cttctacctc ttgcctagtt
     5941 gaaaaatcta atgcaataat tggtcgttgg tctagttgat tcatcgttca cgttcctctc
     6001 tcacttcttt gattaatctt tccagcgatt cgatgcctag ttcgctcatt cgcacaggga
     6061 gctctttaat tagtttagga cagatatacg gatcggtgaa attcgcggtc cctatagcta
     6121 cagcacttgc tccagccata aacatctcaa gaacatcatc cactgtctgc acgccgccca
     6181 tcccaatgat tggaaggctg gatatacttg ctacttggtg aatcaaacga atagcgacag
     6241 gcttgattgc tggtccggag agtccacctg tttgattggc taaaatcggt tttcttgtct
     6301 taaggtcaat ccgcatacca agaagagtat tgatcatcgt aaacccatct gcccctccag
     6361 cttcgattgc ctgagcgatt ggaacgatgt ctgttacatt tggggacaat ttgacataga
     6421 caggaacaga agaaactttc tttactgctt gtgttaattg aaaagcaaca tcagaatctg
     6481 ttccaaaagc aattccgcca tgtttgacat ttgggcaaga aatgttcagc tcgattgctt
     6541 tcacatttgg tgcttcgcca attttgctac aaacttctac atagtcttct tcacaagcac
     6601 cagcaacatt tgcgatgatg ggtaactcat tgtactcctc taattctgga agaagtttat
     6661 gcataacaac gtctagacca ggattttgca aaccaattgc attcaacatg ccagatggtg
     6721 tttccgccac tcttggcgtg ggattgccaa agcgggcatt tggagtcgtt gccttgacca
     6781 tgatcgatcc aagctgattc aaatcatagt attttccgta ttctttgcca aaaccgaaac
     6841 agccgcttgc cggcatgata ggatttttta attctaggcc aggaagtttg accgctaaag
     6901 gattctcatt catataatta cctctcctat tttaaaaatc ggtccttcgt cacaaacttt
     6961 cacacttttt gttcctgttt catcttccgg cgtatgacaa acgcaagcat agcaagcccc
     7021 tatcccacat gccatgcggg attctaatga taactggaca ttttgatgcg aagaaaacag
     7081 ctgttccact gtcttcaata acccgtttga accacatgca aaaacagctg taggttccgt
     7141 cttcgttcct aacaataaat taccgacatt tccgtgcata ccgtatgttc catcgtcagt
     7201 agcgattctt gtttctccta gacgattaaa ttctttttca taataaacga cttcatgtgt
     7261 ggcaaaacct aaaaaatgcg ttggcttaat tcctttttcg actaactgtt ttgataactc
     7321 atatagagga gggacaccga ttcctccacc gattataaat acttcgtctc ctgtatgaag
     7381 attggacaaa tcaaaaccat tgcctaaagg acccatgaca tctaaatggt ctccggcaga
     7441 aagagtagag aagactctag tcccctcacc ttccacccga taaatgatac ggcaagtatt
     7501 tgtttcgtgg tcgatatgat tcaagctaat cggtcggcgt aatagcaagt ctgcacgcgg
     7561 cacgagaata tgcaagaact gtcctggttg ctgcatttct tttactagat cacctttcaa
     7621 tgtcatttca aaaatctttg gtgccagctg tttttgctga acgatttcca tcatttcttg
     7681 tttcatctgt cccctcctgc aacggttttt cgtctttttg tcttaacatg tgctcgccgt
     7741 taaatagggt tagttgaaaa agcacgggat tccatcactc gaacgatcgc atctgctgtg
     7801 tctaaagaag taaacaatgg aataccatgt tcaactgctt ctcttcggat gatgaaaccg
     7861 tctttagaag catttccccg atctttatca atcgtattga caaccacttg tgttttacca
     7921 tctcttatta agtcgactac atttttttct gctgcttcct cagaaatttt cgctactgtt
     7981 tcaacgacca gaccattttt ttcgaaataa gcagctgtat gtttcgttcc tatcagacta
     8041 tagccaattt ctgtaaaacg tttagctaat tctagtgctt cttcttttgt ttcgtccgca
     8101 atcgtaaata atacggctcc atattcaggc aagtgtaatc cagaagcttc gaatgccttg
     8161 tacaaagctt tgtctaattg tctgtcagat cccattactt ctccagtcga tttcatttca
     8221 ggccctaagt aagtatcaac tttctgcaat ttcgtaaaag aaaagacagg tgctttaaca
     8281 tgaacttctt ttgtttctgg ataaagtccg ttttcataac caagtgtctt tagtttctct
     8341 cccaaaatag ctttcgtcgc aatttgtgcc attggaattc ctgtcacctt acttaagaat
     8401 ggaacagtcc gacttgctcg aggattgact tcgatcacat aaacctcatt ttcatggatg
     8461 acaaactgga tattcatcat cccgacacaa ttcaaaccta atgctaagcg gatcgtgtaa
     8521 tcttcgatcg ttttcttgat cgattcagat aatgtttgag gtggatacac agccattgaa
     8581 tcccctgaat ggacaccagc acgctcaata tgctccatga tccctgggat gagtactgtt
     8641 tcaccgtcac aaatcgcatc cacttcgcat tcaccaccaa gcaagtaacg gtccactagt
     8701 actggatgtt ctggagatgc tttcacagca tgttccatgt aatcttctaa atctcgctgg
     8761 ttttcaacga tttccatcgc acgtccgcct aatacatagc ttggacgaac aagaactgga
     8821 taaccaattc tcgaagcgat aaccacggct tcttctttac ttgtagcagt atcccctaaa
     8881 ggttgaggaa tatccaactc tttcagcgct tgctcgaata aatcacgatt ttccgcacga
     8941 tccaagtctt cgatcgttgt ccctaagatt tttacaccag cagcagtcaa aggttcggct
     9001 aagttgattg ctgtctgacc accaaattga acgattactc ctatcggctg ttctagctcg
     9061 atcacattca tcacatcttc gaaggtcaat ggctcaaaat ataatttatc cgaaatagaa
     9121 aagtcagtcg aaactgtttc aggattgctg ttcatgatga ttgcttcgta tcctgcctgc
     9181 tggattgctt tgacagaatg cacagtcgca tagtcaaatt caaccccttg tccgatacgg
     9241 ataggaccag atcccagtac tagaattgag ggcttttctg atcgtgaact ttcattttca
     9301 tattcatagg aactataaaa ataaggtgtt tttgattcga attccgctgc acaagtgtca
     9361 accatcttat atacaggaat caaatcattt tcttttctca tctggcgaac ggcttcttcg
     9421 tttatttccc acagctccgc cacttttttg tctgtaaaac cgttttgttt cgctgttttc
     9481 aatatcttgt catccaacgg ctgctgtttc aactgctgtt cgatttctac gatatgcaac
     9541 agtttatcta aaaagaataa atcgattttt gtcaaatccg ctaattcttc tatggtgtac
     9601 cctcttctaa tagcttcagc tacataaaac aggcgatcat cttgtgctcg aaccactttt
     9661 tggattaggt catcgtccgt gatgtctgcc aactcaggaa gttcgatatg gtgtgtaccg
     9721 atttctaaag aacgcacagc tttcaataaa gactcttcga tattccgacc gatagccatc
     9781 acttcaccag ttgctttcat ctgcgtgcct aaaagccgtt ctccgttttc aaacttatca
     9841 aacggccagc gaggaatttt agctacaaca taatctaatg caggttcaaa ttctgcaaaa
     9901 gtcgttcctg taacaggatt tttcatttcg tctaatgtta atccaagcgc tattttagca
     9961 gctagcttag cgatcggata gccagtcgcc ttacttgcta acgcagatga acgagaaaca
    10021 cgcggattga cttcaatcac ataataatta aaactatgtg gatcaagagc gagttggaca
    10081 ttacagcctc cctcgatctt aagtgctcga ataatcgaaa gagaagcatc acgaagcatt
    10141 tgatattcat ggtcagacaa tgtttgacta ggggcaaata cgatagaatc acctgtatgg
    10201 atacccaccg ggtcaaaatt ttccatattg cagacaacga ttgcattatc agccgaatct
    10261 ctcatcactt catattcaat ttctttaaaa cctgcaatac ttcgttcaat caaacattgg
    10321 gtagcggggg aaagacttaa tccattttct gatacgatac gtaattcttc ttcattttca
    10381 cacatacctc caccagttcc gcctaaagta aaggctggac gaacgatgac aggatagccg
    10441 attttatttg caaaatctac agcttcttct actgtattga cgatttcaga ctcggggatt
    10501 ggctgattca attctttcat caactgtttg aatagatcac gatcttctgc ttgatcgatc
    10561 gcagaaagct ttgttcctag aagttctatg ttcaattctt ctaaaatacc tgatgctgcc
    10621 agctccattg ccatattgag gcctgtctgt ccgcctaaag ttggaaggag agcatctgga
    10681 cgctcttttc gaagaatcct tgatacaaat tcaagtgtga tcggttcgat atatacttga
    10741 tctgccactt ctttatccgt catgatcgtc gcaggattag aatttactaa aaccacttca
    10801 tatccttctt cttttaaggc aaggcaggct tgtgttcctg cgtagtcaaa ttctgcggct
    10861 tgaccaatga tgatcggacc agatccaatc accataattt tcttgatatc tgtacgtttc
    10921 ggcatctgtt tactgctcct ttcctgcgtc catcatttcc ataaattcat caaataaatg
    10981 tagtgcatca tgcggaccag gggcagcatc tggatgaaac tgcacactga aagcgggata
    11041 atctctgtgt ttgactcctt ctactgttcc gtcatttact tctacatgag ttacgatgag
    11101 tttttctgga tcaatcgtgg cttcatcgac tgcatagcca tgattttgag aagtaaaatc
    11161 aattcttccg gtcgctactt ctcgtaccgg gtgatttaat cctcgatgtc cgaatttcat
    11221 tttataggta tctgcaccgt tggctaaagc aaataactgg tgaccgagac aaataccaaa
    11281 aatcggtact tttccttgga tttttttgat catttcgatt gcttctggca catcttttgg
    11341 atcaccaggt ccattcgtca gcatgactcc gtctggtgat agcgctaaaa tcgtctcggc
    11401 atctgtgtta tagggaagta ctgtgaggtt acagtttctt ttactcaatt ctcgtaaaat
    11461 actatgtttc aagccgaagt caatgacaat gactttccga cctgttcctg gacttggata
    11521 aggtttgtta gttgagactt gctgcacttg attggttgct agaacggttg ctttcagctg
    11581 atcaaatgca tgttcgaagg aatctccagc atcaactata ctagctttca tcgttccgac
    11641 ttcacggatt ttgcgagtaa gggcgcgcgt atcgatacct gatattccag gaattccttt
    11701 ttgtttcaaa aatgaatcta gcgtcatttg gtttcgccag tttgaagcta cacgtgcatg
    11761 ttcttttact actactcctt tgcatgttgg agaaatcgat tcataatcat ctctatttac
    11821 cccataatta cccaccatag gataggtaaa tgtaatgatt tgaccgttaa aactttgatc
    11881 agtgatagtt tcttgataac cagtcatact tgttgtaaaa acgatttctc cgaagacact
    11941 agcatctgca ccaaaagcct ttccttcgaa aactgttcca tcttctaata taagcaaacg
    12001 ttccatactt ttattctccc ttctgccaca caagttttcc gttgacaaaa gtcatttgtg
    12061 tttcgccttt tactttccag ccgataaaag gtgtattctc cccttttgat aaaaagtctt
    12121 ctttatcgat cgtgcaagtt tgtgtaatat caaaaactgc tacatcagcg ggggcaccaa
    12181 ccgttagcgt tcctgcattc aaaccaaaaa tctcagccgg tttaactgcc aaccaattga
    12241 tcacttgttc caacgtgaat cgaccagttt ctacaaaatg agtatagatc agttgaaacg
    12301 ctgtttcgct tcctacgata ccaaatggtg atttcatgaa actttggctt ttttcttcta
    12361 atccatgggg ggcatggtct gtagcgatgc aatcgatcgt accgtcaagc aagccttcga
    12421 tcaaagcttc tctgtcctcc cgtcctctta gcggcgggtt cattttccaa aagccgaaat
    12481 cttcgggaat atcttcgtcg atcaaaataa gatgatgggg tgaaacttct gctgttacgt
    12541 ggattcctgc tttttttgcg tctctgatta cacggacact ttctttagta gaaacatggc
    12601 aaacatggta atgaacacca gttgcttcag ctagcaacag atctctagca atttgagaag
    12661 actcggttac gcttaagatc ccgggaagac caagttcttc tgccttcttg ccagcgtgca
    12721 taacgccgcc aaataataaa gattcatctt ctgtatgggc tactagggct ttattgtttt
    12781 ttgcagcttc tttcatagcc agatacatcg tcccagctgt ttggacacct acaccgtcat
    12841 ttgtgaaagc aaaagcacct tcttcaatca gtgcttcttg atcaactaat ttctcacttc
    12901 gcagattttc tgtgattggc gcatactgca ataccttcac aactgcatct tttctaatga
    12961 tgtcatatac ttgtctcagc ttttctgccg tatcaggtac aggatttaaa ttcggcatcg
    13021 cgcatacagt agtgaaaccg ccacgagcag ctgagcgagt cccagcttcg atcgtctcct
    13081 tgtatgtaaa tcctggctct ctcaaatgaa catggacgtc cactaaacca ggtgtgatca
    13141 attgtccctt tgcatcaaac acttcatcaa attcggcttc tgagaatcct gtaccgattg
    13201 ctttgattcg cccatcttct atccaaattt ctgctggtgt cgtcatattt tttcgtgtat
    13261 tgatctgacc gtttttaatg agtgttttca ttttttcatc ctcctataat tacgcttttc
    13321 catttagcac tgcttctaag attgccattc tgacgaatac tccattgctc atttgctgaa
    13381 cgatccgcga ctgtaatcct tccactaatg aatcagctaa ttctacatca cggttaactg
    13441 gtgctgggtg cataatgatt gcatgttttt gcaacttctt cgcacgctca actgttaacc
    13501 catgttcctg atggtagcct tcttttgaga aactttcttt tccatcatgg cgttcgtgtt
    13561 gcacccgaag catcatcatg acatcaactt gatctagtag ttcatccaat ggcatataat
    13621 gtccgtacac ttcgaattca tcgtcgtacc attctctagg acctgagaag aatacttgtg
    13681 cgcctagacg ttttaacatc tgcatattgg atttagctac gcgagagtga gtgatatcac
    13741 ctacgatggc aactttcaga ccttcaaaat gaccaaattc ttcataaatg gtcatcaaat
    13801 caagcaagca ttgggttgga tgctgtccgc ttccgtctcc tccattgata atcgaacact
    13861 ggattgtttt actttgtatc agttcgtcat aatagttctc atcaccatga cggataaccg
    13921 ctacgtctac gcctaatgct gacatcgtca gtactgtatc gtatagtgtt tccccttttt
    13981 ggacagagct tgtgcttgct tcaaattcaa tcacttctag ccctagtttt ttctccgcta
    14041 catcgaagct tttatgtgtg cgagtactat tttcgaaaaa gaggtttgta gcaaagtact
    14101 ggcgcttttg cggagtccaa ttagcacctt gcttgaattc ctgccctctg cggatcaagc
    14161 ccataacttc ttgatcagta agtgcctcaa cggtcaataa atgctttaag ctgatacgtt
    14221 ctgatttgat gatcataatg tttcctcctt agttttcttc gcttcgtgaa gtttctggta
    14281 agataagatt taaaatgatg ccaagtacag ttgctaatgc cattgccgac aatgtaaatg
    14341 tgcctacttc aaagacgagt ccgccaattc ctactactaa gataactgaa gcaatcaaca
    14401 gatttttctt tttatcaaaa tcgattttgt tctcgattaa aatcttcatt ccacttgcgg
    14461 caattactcc aaataagata aaactgattc ctgagatgac tggtccaggg atacttaaaa
    14521 tcaaagcgct taattttcct acgaatccta aaatgactgc aaatactgca gctccaccga
    14581 tcacaaacac gctatggaca cgtgtgatag ccagaacacc aatgttttca ccgtaacttg
    14641 ttacaggagg acctccgaca aaggctgcca cgatttgtgc taatccatca cctgtcaacg
    14701 tcttatgaag acccggttct tcaaagaaat tacgtttagt tagtttattc aataccataa
    14761 gatggccgat atgctctgtc atcgtcacaa aagcgatggg tgccattgtt gtgattgctc
    14821 caagatatag ctttggttca tattgtacaa agggaatctc aaagttagga acagcaaacc
    14881 aggcagcgtc tacgattggc ttagtatcca cgattccaaa cagcaaggct acgatgtatc
    14941 cgctaataat acctagtaaa atcggaatca atccgagaaa acctcttagc cacatgttga
    15001 agaaaattgt taatcctaat gtgatcagcg ctacagcaat atacttgaaa tcatacgtgc
    15061 cctgattata catagcattt gttgctgcat ttgccgcaag tcccaacccg ataaccatga
    15121 tgactggacc gacgacgatt ggaggcaaga ttttatccag ccatgctgaa ccgatctttt
    15181 tgatgatcag cgcaacaatc aaatagacta atccagttgt gactgctcct tgtgcaatcg
    15241 ctggataacc atctgttttc atcaaaagct gcatcgctgc aatgaaagcg aagctgcttc
    15301 ctaagtacgc aggtatcttt cctttagtag taaataagta caccaatgta cctagtcctg
    15361 aactgactag cgcaatacct ggatcgatac ctactaagat ggggactaac acggttgctc
    15421 cgaacatcgt gaacaagtgt tgtaaactta atcctaccca atgtcctggg gttgggcgat
    15481 cgtggatatc taacaccacg tcatcattat gaaatccttt tttctccgac atgtttctct
    15541 ctcctctaat cttcctcttt caaaatcatg atgcgatctt gtccatcaag ctcttgcatc
    15601 tctactaaaa tctcttccgc ccgagatgtt ggaatgttct tcccaacata atctgcacgg
    15661 attggcaatt ctcgatgccc acgatcgacc aggactgcta atgaaatttt tcttggtctg
    15721 ccaaaatcca ttactgcgtc cattgctgct cgaatcgttc gtcctgtata caaaacatca
    15781 tcaatcaaaa taacttcttt tccttctaag gaaactggga tgtcagaaga atgaagttcc
    15841 ggttcttctg gattttcttt tttgtcatct cggtataaag tgatatccag ttctcctaca
    15901 ggaatatcga tatcttctag ctgcttcagg cgttcggcaa ttcttgatgc aatgtagatt
    15961 cctcgtgttt tgatacctac caatacgata tcttggatac tatggtttct ctcgataatc
    16021 tcataagtaa ttcgagtcaa tgctcgcttc atggttacct gatctactac ttcttttgct
    16081 tgcattttca ttccccctac ttttttgcat aaaaaaactc ctgccctaag aggacaggag
    16141 gaatgtttag actgagcgta tactgataca ctgatagcta tactatgtca atgagcaatc
    16201 tacttatggc gcaccatacg tatccttgct cccacccttc ttagcctcac gggactaaac
    16261 ttaaaggaat tctattaagt tttatgcttt cgttgttaat tactataccg ttatctaaat
    16321 taaaatgcaa tgttttttct taatttttct aaagtttttt caaaaacttc tggaagaggt
    16381 gcttcaaaga tcatcttttc gtttgtagtc gggtgcgtaa aacctaacat ttgcgcatgg
    16441 agaaactgac cattgccttt caaagttttc ttcggtccgt atactggatc tccagctaac
    16501 ggataaccga tatacttcat atgcacacgg atctggtgtg ttcttccggt ttctagtttt
    16561 aattcaacca gagtaaatcc ttcgaaacgt tccaatacag taaaatgtgt gacagccgac
    16621 ttcccatctt ctcttatcgc ctgcattttc cggttttcct tcgaacggcc gattggcgca
    16681 ttgatggttc ctttttcgtg aggaatctct ccatggacta aagcaacata tttgcgcaaa
    16741 gaggtcttat cttttaattg ctgagctaat gattcgtgtg cctggtcatt tttggcaacc
    16801 attagcagtc cggaagtatc tttgtcgatt cggtgaacaa tacctggacg aataacgccg
    16861 ttgatcgtag aaagatcctt gatatggtac atcagcgcat tgaccattgt gccatgcgta
    16921 tgacctgctg aaggatgaac gaccattcct tgaggtttgt ttatcaccgc tacatcttca
    16981 tcttcgtaaa cgatcgtcaa cgggatattt tctgggacca catctaatgt ttctggttcg
    17041 gggatcaaga cagtaatctc gtctccttgt tttactttgt aatttgcttt gactggctgt
    17101 ccattgacag aaaccgctcc atttttcagc cacagctgaa tttgcgaacg actgtaatcg
    17161 gtgaataaat tagtcaatac tttatcgatc ctaccagatt catggtgaat cgtcgtttta
    17221 agttctgtca tttttttctt tgatcctttt catctaaaat aatataaatt aacatacaaa
    17281 tcactccaca tacaagtgtc acatctgcta cgttaaaaat cggaaagctc ataaaatctg
    17341 tctggaacat atctactaca tagccaagac gaatacggtc gataaaattg ccaatcgcac
    17401 cagctagaat caaactcaat ccaatcatga accagcgatt acctttttca cgatttttaa
    17461 tcatcaatgt gagaatcaca acgactgcaa tgagtgtcac tatagtaaag aaccacatct
    17521 tcccttctag catactccat gcggctccag tgtttcgaat atgattcagt gacaaaatac
    17581 ctggtataag atttttcgtc tctccaagcg ctagattatc agtgatccac catttgacca
    17641 actgatccac tcctactagc aaagcgctga atatccaata aataattaac aaaaaatcaa
    17701 tccttttcta tttgtttata aatatcacga ggtcgtacga tcccgagcag attaccatcg
    17761 ggattcccat ctttagtgat aagtacggct tccaaagtcc cttgctcacg aaacatcgct
    17821 tcaacctcgt aaatatgtgt tttagctgga acaaaatgat aatttgtgta ctttccgttt
    17881 tggatgatca aatccaacgc tcgtttattt gttaaatcaa ccggaccttt ttggctttct
    17941 tttgctagcc aaaacccgat attacgtaaa gtaatcaatg ctacaaattt tcctttattg
    18001 tataacggaa attgtgaata ccttttttct gctactatct ttaacagttc aagtaacgga
    18061 agatcttgtt caaaacctgt tacacgtttt gcaaatcgag gcagcacggt ttcaggctga
    18121 atcagttctt gttcgatcag ctggattcgc ttgactgtcc aatcatttgg ctctgcaatg
    18181 acaaaatcga ctgcgatccg atcatggacg atcgcattcc ttaactgagc aagctgtaaa
    18241 agatcatctt cgtattttct gatcattaat tgttttcttt gagctaggcg tctgacaagt
    18301 tcggtaaatc ccatatttcg tgcatttccc atctcgtctc tcatccattt ttcgattcga
    18361 ttgaaactga ttaaaaaaag atcagagttt gccataattt tcctccttga tcaaatatgc
    18421 ccggtttttt atatagaaaa agaacgcaat caacacaata actgcaaaaa aactgaaaaa
    18481 taaattttgt aggaaaacgg cccccaaccc agttgctaga aataaaaacg gatagatttg
    18541 aagaatcaag taattcattg tgatcacata agcaggcttt gcttctttca ctgtataaac
    18601 ggggcttttt agccaataaa aggtgacctg catcgtaaag ggcacttcca gatcaagcgt
    18661 tatcttttgt tcatgactga gaacactgcg tttttcattg ttgattctta gctgaatcga
    18721 gctacccata ccgtaaaaac cggtttttct tataaatgtc gttttcatca taccctccta
    18781 ttatgcttta gtatagctca aaatacaata aagaaaaaga atgatcctag tgtaaacaaa
    18841 attaattccc tatcgtctaa aaaaagaagc tgtagtcaaa atacagctcc ttcgatggta
    18901 gattataatc aagcttaaaa caatcccaac acttctccgt catttgtcac gtccatattc
    18961 aatgcagcag gttttttagg caaaccaggc atcgtcataa catctccagt caaggctact
    19021 aaaaatcctg cccctaattt tgggatgatt tcgcggatcg tgatggtgaa atcttccggt
    19081 gcacctagca gtgaaggatc atcagaaaat gaatactgtg tttttgccat acaaatcggc
    19141 agtttgtccc atccattctt cgtaaactct tctatttgtt tttgcgcttt tttgctgaaa
    19201 acaacaccat ttcctccgta aattttcttg acgattgttt ctgtcttttc ttgtatcgtt
    19261 tcttctaggc ggtataatgg cttgtaatta gcttcttttc tatcaatcaa tcgtactaca
    19321 gcttttgcta aatcaacgcc gccttctgct ccatctgccc atacactcgc tctcttggca
    19381 agaatcccct gattttcaca caagtgctcc aacagtgtga gttcattgtc tgtatctgtt
    19441 acgaattcat tgattgcgac gatcacaggt agctgatatt gttccatatt acgtatatgg
    19501 cgctttaaat ttgcaaaacc tactttcaaa gcatcaagat tctcattttt aagttcatct
    19561 tttttcattc cgccatgcat tttcaatgca cggacagtgg cgacaatcac cacagcatcc
    19621 ggcgcttttt tcaagttagg tactttgata tctaaaaatt tctctccgcc aagatctgca
    19681 ccaaatcctg cctctgtgac aacgtaatct gctaatctca atgctgtctt ggtagctaaa
    19741 atactattgc agccatgagc gatattggca aacggtcccc cgtggacaaa agctggtgtt
    19801 ccgtaaattg tttgtacaag attcggcttg attgcatctt tcagcagtaa tgccagagcg
    19861 ccttctgctt tcaagtctcc agcagtcaca ggctgacggt caaatgtata gccgacaacg
    19921 atccttgcaa gacgagcctt taaatcttca aggtctgccg caagacacaa gatagccatg
    19981 atttcgctgg caactgtgat atcaaaccca tcttctcttg gaacaccttg gattggccca
    20041 cccaagccaa caattacttt acgcagttca cgatcattga gatcgactac ccgtttccaa
    20101 atcactcggc gggcatcgat gttcaattca tttccttgat gaatatgatt atctaaaaga
    20161 gcagataatg cattattagc tgttgtgata gcatgcatgt caccagtaaa atgcaagttg
    20221 atatcttcca taggcaatac ctgtgcatat cctccgcctg cagcaccacc tttgattccc
    20281 atcacaggac caagtgaggg ttctcgcaat gcaataacag attttttatt gattttattg
    20341 agcgcgtctc ctaaccctac agtgatcgtt gactttcctt cgccagctgg tgttggattg
    20401 atggaagtaa ctaagatcaa atgcccctca gcgttatcct ttgatcgatt gattccagaa
    20461 aaattgattt tcgccttata tttcccatac agttccaaat cgtcttcctt aagatctaat
    20521 ttactagcca cttcaacaat cggttctagc tctgtttctt gtgaaatttg cagatcattc
    20581 ttcatttgat tacccctttt cacgaacttt attcaacaaa atatcttctt tgttaagata
    20641 tgccaattat accttttttt attcagaaaa aaaacataaa attacaggtt tttaatttat
    20701 ttgacgtact tataaatact ctttataatg aaggctatga tcagacttta gaaaggtcgt
    20761 gagtgctttg accacaggaa ttgtaaaatg gtttgacaac aaaaaaggat acggctttat
    20821 cagttatgat gacacagaag aaatttttgt acattttact gcgattgaag aagagggatt
    20881 caaaactttg gaagaaaatc aagtggtcga atttgagatc atcgaaggga atcgaggaac
    20941 acaagctgcc catgtcaaga aaggcaacgt tcagactacc gatactagta tgcatgaata
    21001 gataaacaaa tagagtgaga cgctaaacgg gatgaaccgt acgtctcact cttttctttt
    21061 cctgtaaaaa ttcgtttttt taggttctga tttttaaaat tagctttttc tttttttcgt
    21121 ataaggataa ggaattgatt ctgatttggg tccgtttttt tcaaaatgat caaaaaggac
    21181 tacttgatct ttttttcgca tcatgaaaac agatggggct ttctcatctt gcaacatcat
    21241 ttgcacccca gtattagttg gcgtcaaaga tcgaatctgt ccgacagata tttctcggtc
    21301 tttccaaaaa gcagcataac gtataatcac agagtttgat tttaaaataa aagaacgccg
    21361 caaacctaga aatagaaaaa caagaaaaat acctaaaaca aaattgcttt tccaataagg
    21421 acctgtgttt tctaaagaaa gaatcagact aaaaaacaga atgatcaagg tcaatgacca
    21481 atagatgatc gtatgtgcca gttcaggctg ccatcgaata ttttttttca ttctctaacc
    21541 tctcaccgat tcagtaatga tataatctta ccataacatt agtttgaaca aaaaggagaa
    21601 tctcttttta tgttaaaagt atacattgat gcctctacaa aaggcaaccc tggtcctagt
    21661 ggaggtggaa tcttgattgt ctatcaaggt aaacaagaac agctatctat cccgctttct
    21721 atagggacaa accatcaagc agagttcgaa gtctttttaa aaactttaga atttctaaag
    21781 aaaaacgact tacagaatga aacgattttc tgtttttcag acagcaaaac actagtttct
    21841 acggtcgaca aaaacgcaac gaagaacgag ctcttccttc cctaccttca aagaatccaa
    21901 gaacttctat ccgaattttc tttattgatc ctgcaatggt tgcccgaaaa caaaaataaa
    21961 ggagcagata atcttgcacg tcaggctctc cataaacaat taaaagaaaa aaagactctt
    22021 taaattaaaa actttaagga gtctttttat agtttctcat ttaatataaa agataattgt
    22081 gatcaaacgt aatttccaat tggtaatctc cgattttttc ttctatttga ctacgtaaga
    22141 ttctttctag ttctgtatca acaaactgga atttctcagg gaccactaca tcaaattgca
    22201 gcgtttttcc ttgatgaatt actctaaaat catgaatatt aagccctgta cctgtttcaa
    22261 gaagaatttg ctttaactgc cgcaagatat tatagtattt ttcgttatca acaggcattg
    22321 gatccaagtg acagacaagt tccacatcca aagacttttt aaagtctttt tctatcagat
    22381 caataatctt atgagccaca ttcaaattca ttgattcatc tacttcgatg tggactgatg
    22441 caaagcgttt attgggtcca tagttatgca ccaataaatc atgatagcca agtatatcat
    22501 cgcaagcatt taaatgctct tccatttctt caatctctgc tggcgttggg cgacttccta
    22561 atagttcgta tacaaaattt cgaagcatcg tcaatccact ataaaggata tataatgcta
    22621 aagcaaaacc tatatatcca tcaatacgcc atccagttac ccattcgatt gctgctgaaa
    22681 gcaatacagc cagtgtagta aagacatcat tataactatc tgttgctgta gctctcaatg
    22741 tttcagaatc aatggattgc gcaataacct gatacatttt gccttgtgca aatttcaaaa
    22801 agatggaaat gattaggaca aaaaagacga tgggtgttaa agagacattt tctggatgga
    22861 tgattttttc agcagaagac tctaaaaatt gaaatcctac ataagtaatg atgatcgaaa
    22921 caaataaccc actgatatat tcgaagcgtt cgtgtccata aggatgttcc tgatctgctg
    22981 gctttgcagc aattttaaat ccgatcaaag tcaatatgga tgaagcagta tctgatagac
    23041 tgttcactgc atcagccata atggagacac ttcctgaaaa aagcccgatc attagcttag
    23101 agacaaacaa gatcagattc gtaaccaatc ctaagatacc cgctagtact cctttttttg
    23161 tccgctgttg cggtatttgt ttcttttttt cttcttgttt cttagccaag ttttttaccc
    23221 cttctagttt gcttgttgct caggtaaaaa gatggagagg acgattgttt tcctctcctc
    23281 tcttcctttt tctatctatc gtgcgtccta ttactattaa aagatggtca ttattctggt
    23341 ttcaacggtg ttgctgcaaa taacgctttt gctagtaatg gtgttatgat tcccgcacct
    23401 attgcttctg gtatcccatt gattgctgca acccctgcta aggttttaaa taaaagagca
    23461 gagtctacac cataggcatc tgccgttgca cctgtataaa ataatcccat gaatcctaac
    23521 actaagactg tattgaccaa tgaacccagt acccctgcaa aggctgagct gatcgttatt
    23581 gatttatttt tttgataaat ccagtggaaa accaagcctg ctactaagcc aacaagtact
    23641 ctgggaacta ccgagataat cggattcgtg aacactaatg tatcaaatgg cgttgttggc
    23701 atggcatagg cacgaacaat tgtagcgatt ccccaaaaca agccaatcac catgccatct
    23761 tttgggccta atactactgc agcaataatt actgtgatat ggataattgt taaactaatg
    23821 aatcctaatg gaataaaccc cagccaagga accatcgctt gtacgatgat gattgctaac
    23881 aagatcgcac gtaagactag tcgaaaggtc ttatttctac tattcatttt ttcacccctt
    23941 tttatttata ctaatcgtaa aaatttctgt cactttcgtc aactaaagac catccgaatc
    24001 cgaccatttg attagatgcg ccatccaatt gaaaaaagta tgagggcaag acttgtttgt
    24061 cttcgccccc ataatcatgt ttcgttcaat gtttgaactt ttgtttttct gtcaaacgta
    24121 aacgccctat tgttgcgcaa cccattcttt cgcagcttta agtgcatctg gaataccagc
    24181 aggatttttc ccgccagctt gtgccatgtc tggacgtcca ccaccgccac cgcctacttt
    24241 cggtgcgatt gctttaatca agtcgccagc ttttagtcca gcttcatttg attctttcgt
    24301 cattgccgct agcagactga cttttccttc ttgttcgttt gctaaaacaa gaatatctga
    24361 aatatttttt tgtttccatt ggtcagctaa ttgacgcagc tgattcatat ctttcacttt
    24421 tacttgtgct gcaatgattg tttttccatt gatcgtttct ggatctttga atacatcacc
    24481 agcttgttga ttcgctaact tgctagctaa ttgttcattt tccttttgta gctctcttac
    24541 ttgctgttgc aattgttccg cttttgaaac aacttctttc aattgaggtg atttaacaat
    24601 tgttgctact tgttttagtt gtttttcttc ttcttcaagt agttcgtacg cttctttact
    24661 tgttacagcc tcgattcgtc gcacgcctgc accaattcct gattcagaaa cgattttgaa
    24721 gattccgatg ttttctgtat ttgctacatg tgtgcctccg cacagttcga ttgaccagcc
    24781 gccaatattt actactcgaa cgtcatgtcc gtatttttca ccaaacaaag ccattgcacc
    24841 catgtttttg gctgtatcga tatctgtttc aattgtttca actgggatag cttcccagat
    24901 tttttcgttg acgattcttt ccatttcagc taattcatct gaagtgattt gaccaaagtg
    24961 ggtaaagtcg aatcgtaaat gtccaggagc gactaaagaa ccagcttgat ttgcatgctc
    25021 gccaagtact tctttcaatg ccttgtgcag tagatgggtt gctgtatggt ttttgatgat
    25081 ccgattgcgc atcgattgat cgatcaatag atgataggtt tgtccttcac tcattggtgc
    25141 ttttacttct acggtgtgca taaattgacc attaggcgct ttttgaacat ctgtaacaac
    25201 tgctacgatt tcgcctactt cattcatgat ccatccttga tccgctactt gcccacccat
    25261 ttctgcatag aaaggtgttt gatcaaagat catttgagct tcaccttctg cgacttcatt
    25321 caaaagagac tcacctttaa cgatgaccaa taattgacta tctgattctt gttctgtgta
    25381 tccaatgaat ttactttcta ctttgatatc tgtcaataaa gcagattgta cccccattga
    25441 tttttcagta ctgcgggcag agcgagcacg ttcgcgttgt gcatccattt ctttttcaaa
    25501 accagcatga tcaactttca gtccttcatc ttctgccacc tcttccgtta attctaccgg
    25561 gaagccatat gtgtcgtata gtttgaagat gtcttttcct gctagagtcg tcccgttttc
    25621 gttttttact tcttggatca cttgatttaa gatttccaaa ccttcattga ttgtttcatt
    25681 gaaacgttct tcttctgtac ggataacttt ttcaataaat tctttttgtt gaagtacttc
    25741 aggataataa cttaccatga tttccccaac aacagggact aaacgatata agaatgcttg
    25801 ttcaatacct aattttttcc catgcatgac agcacgacga agcaaacggc gtaagacata
    25861 ccctctccct tcattcgatg gcaatgcacc gtcgccaata gcaaatgaaa gggcgcgaat
    25921 atgatctgcg attactttgt aagaaatatc tgttactggg ttttcaccat attttacttg
    25981 tttgcttagc ttttctacgg cgtggataat aggcataaac aagtctgtct caaagttcgt
    26041 tgacgcatct tgaacgattg agaccatgcg ttccaatccc atccccgtat caatgttttt
    26101 atgaggaagt ggttcaaatg tgttatcagg cttatggttg aattctgaaa atactaagtt
    26161 ccagatttct aaataacgtt cattttctcc tcctggatag ttttcaggat cgtcttctgc
    26221 taaatcgtta taagcttctc cacggtcata gaaaatttcc gtatccggac cacatgggcc
    26281 tgcaccgata tcccagaagt tgtcttctac atcaataatg tgatctagtg gcaggccaac
    26341 ttcttcatgc cagatgcgtt ttgcttctgt atcttttgga taaactgtta cataaagttt
    26401 ttctggatcg aatgccatcc attttggatc agtcaaaaat tcccatgccc agtggatggc
    26461 ttcatttttg aaatagtcac caatcgaaaa attacccagc atctcaaaca tcgtatggtg
    26521 ccgtgctgtt ttaccgacat tttcgatatc atttgttcga atagattttt gggcatttgt
    26581 gattcttgga ttttctggca caactgaacc atcaaaatat tttttcaatg ttgcaacacc
    26641 agagttgatc cacaataagg ttggatcgtt cacggggatc aaagacgcac taggttcaat
    26701 cgtatgtcct ttagattgga agaaatcaag aaacatttgt cgaacttgtg cactacttaa
    26761 ttgtttcatg tcattacctg ctttctataa attaaataca aaaaaacacc atcacagcca
    26821 aggacgaaaa ctcgcggtac caccttgatt gcaatgatgt gtaatcatta cctcttaaac
    26881 tcgataacgc tgagctgcgt cggtatttct accaaaatag aaaaagggag caatttgatt
    26941 agtgactatt cttttttcag ccaagaaaga attttctgtg agtcttaaaa aacaaatcat
    27001 atccttttcg tacttaatta gtatacgcat tctattgatc actgtcaata tctcaatttt
    27061 tattttcatt tccttttggt catactccac gatttttggt acgataagaa taaaggaatc
    27121 aatacatctc tgctattcat gacaaggagg aatatgatga aaaaaatagt tggagcggat
    27181 gaatggatac gcgcagctag ctgctttttg agatatcaag tttttgtatt agaacaaggg
    27241 attcctgcag aactagaatt cgacggaaaa gatatcccgc aacaaaccta ttatctattt
    27301 cttgatcaaa aaagaccagt agctactttg cgttatgaaa agaaaacacc tacttgcttc
    27361 aagccggaac gattttgcgt agcaaaagaa taccgaggaa aagggttagg cggtcagttg
    27421 cttgaagctg ctgaaaaacg ggcggtttca gaaggttgtc agactagtta tgtagtagct
    27481 gaaatgacgg ctgtagattt ttatgaaaaa caagggtatc atacttgcac agaacctttt
    27541 attgaagatg aagtactttg tatcggaatg aaaaaaagtc tagcttaaaa acaagagatt
    27601 gtgacacgcc ttttgtcaca acctcttgtt ttttatttat gtgaattttt cttattttta
    27661 cgagcagtac ggctttgctg tctgcgttcg attttgcgtt cttttttatt tttttctgca
    27721 attgcccaat cgattttttt cttataacca ggcttgatct ttttcttttt cttcttcaca
    27781 agaccaatca gcgtgggatc taatacatct tttgattttt ctcgtttggc acgtctatta
    27841 cgatcgtatg ttttgacgat ttccccgttt ttgatttctt ttggttcaaa tgagacgccc
    27901 aatttttcta tcgcatcaat ggcttgctca tcatttggcg aatataacgt aatggccgtt
    27961 ccttttaagc cattacgtcc agtacgtccg acacgatgga taaagaagtc tagatcttct
    28021 ggaatttctg cattaataac atgagagaca ccttcgatat cgatccctct agcagctaaa
    28081 tcagtcgcta caacatattg atactccaga ttctgtactt gtcgcatcac tcgttttctc
    28141 tctcttgggg tgatgtctcc atgaatcttt gctactttca agccttggtt tttcaagtaa
    28201 tcggcaattt catccacatg ctgttttgta ttggcaaata cgattgctaa ataaggatga
    28261 ccaattgtca gcagttgata aatcatttga ttctcatctt taccttttgt tgaaatcagc
    28321 caattttcaa tgtcttcaga aataactgct tttggtttga tttcttcaat gattggattt
    28381 tccatgtatt ttcgtaagaa tggacgaagt ttttctggaa tagtggcaga aaaaactaaa
    28441 aactgcagct tttctggtaa tcgacttgca atttggtcta cttcatttaa aaaacccata
    28501 tccaatgtca tatccgcttc atcgacaaca aatgcaaatg cggtgtgaat cttcagcgct
    28561 tgttcattca tcatgtctaa tattctacca ggcgtgccta ctacaacctg tggctgttgg
    28621 tgatgcagac gatttaattg acgctgtttg tctgtaccgc cgacaaaatt cgagacacgg
    28681 atctccggtg tagaaaattt agcgatttgt gtagctgctt catagatctg tgcagctagt
    28741 tccctacttg gagctgtaat aacaacttgt acttcctctt tttctgcatt gattttttca
    28801 aacaaaggca acaaaaacgt atgtgttttt cctgtaccag tttgagattg cccaatgata
    28861 ttttttcctt ttgcaattaa aggaatcaaa cgttcctgga cctctgtggg ctgtttgaag
    28921 ccctttgcgg ttaacgcttc attgataaac ggtttgaagc ggaatatatc aaaattattc
    28981 attcgtgcac ctcttctctt taaattatca cgactttggc attataacac agacaaagcg
    29041 tttttgctca gctaatttcg agaatcatca tcctttgttt ggccaatcgt gctaacagta
    29101 tatgtgtgac tagctttttc ataaaacaga atccgttttt ttctcaaaaa tgatagcaag
    29161 atatttaaaa taaaaatacc ttgtagtaac aagacaccga gagaaatgaa tactgcactc
    29221 atctgcaaca ccgtattcgt tgtcgaattc atgatttgct gaagcaactg ccccaaccag
    29281 cttaaaccat aaaaaagaaa gtacagatac ccgtagcgga tcatcaatgc ttttaaagaa
    29341 attcgttcgc ggtcttcttc caccaaacgg atacgcacaa tttttttgcc aagtgtccat
    29401 ccattggtta taaacggtac aatcataaag tataccccta cttctaataa aagccaccag
    29461 atatgagaag taactgcccc tgaatcgaac gcaattttaa agaaaatcgc cgctaccaaa
    29521 ttaataagag ataaaaatgt ccagtcgatc aaataagcga ccaagcgtcg agtcaatgag
    29581 acagtctttc ctttttcata agaaagttca tccattcgtc ttcttgtagg aagcataaat
    29641 gtaaataacg gcgtaacgac aaaaccaatg attccaccca atgtatttgt cactaaatca
    29701 ttcacatcaa ataagcggta tggtcttggg tagataaaat aaagtccact tagctgcgtc
    29761 aattcaaaaa atagagaaag gcagaaacta gcaatcatcg ttttcataaa agaaagccgg
    29821 aaataatatc ttaaataaat gccaaacggt atgagtaata aaacattaaa agctggttcc
    29881 aaaaatacac tctgcttcat tgccggcagg taggtactag gttcagacaa gaccaatacc
    29941 gtgttgtctc tgaaatgttg ccatgaagca aataaatgca gctccatctt tggccctgtc
    30001 atttgggcaa ccgtctcctt tgccggaagt ggtaagatca ccaaaaaata cgcacacaat
    30061 aaataaaaaa cgaaagaata caaaataact gcccttgtta ctagaaatgt tccatacttt
    30121 cgatattcat aaataaaaaa aacagatgag atcgctaagg ctaaaaatgg aaaaacaatc
    30181 agcgcaaaac gaatcggttc gatataactt gccatatgac tcctccttga ttctttgtat
    30241 ctctcttatc ttagcagtcc tttgctgttt caagcaagta acgaggctat tatttctcat
    30301 ttctttcaaa gtaaagtaag ataaacacaa agggggtgtc gagatgctga atatgtactt
    30361 tgtttttggc gtacctattt ttttactgtt cctttatgct acgatcgctt atgtaagaaa
    30421 aagaactaca attcattatt taggatttat tctcctgatt atttcgggtt tcatgctagt
    30481 cttcaatctt cagacttggc agcaagcact tcttgaaatg gataaaatga ctccacatgc
    30541 tttgtctaaa gtgcttggct accctgtcta tctgatttgg cttcccattt tcatttctgg
    30601 ttgcttggtt ttattaaata tttatcgcgg cgtacgccga atagttcagt tacgcaaatc
    30661 aaagtaaaag acagaaatcg gttgagcccg atttctgtct tttactttgc tatttcatcg
    30721 tagacaaaac gacatttacg tgatcaaaat taacgagtac ttttgacatt tcttccacgt
    30781 actcctcatt taaataaaat attttgtttg gcagagaagt gcagttctaa taaaattcga
    30841 atgttttagc tatctacgtt ttaaatagag ataaatcggg ataccagcaa gtgtcaatac
    30901 tatccccatc gaagctaaaa aggtctgagt gataagcgtg cttgatacga taaaagtccc
    30961 tccaatgatt gctactagtg gaataaacgg atacattgga actttgtatg gtcgaaacaa
    31021 atcaggttct tttttacgaa gaagaatgac accaacaaag accatcgtat aaaaaatcca
    31081 aatgacaaag atcagcatat ccgttagtgt atcaaaacca ccgatcatca tcatgccaat
    31141 agcgatgact agctctaaga tacctgcggc tacaggaact ttagttttat catgaagttt
    31201 agcaaagagc ttgctaaaag gcaggttgtt ttcttttgcc ataacataag ggagacgcat
    31261 tccagtaagg gtatagccat tgatggttcc ataaacagaa atcaagatac caactgtgac
    31321 cagtcttcca ccgaagccgc caaaaatcat ttttgcaacg tcacttgccg cattgctgtt
    31381 tcctgcgact ccgtcgatag atgcggttct taagaacacc gcatttacta ataaataaac
    31441 gatcatgatt ccgatgattc ctaaagaaat tgcttttggc aaatctttcg ccggcttttt
    31501 caattctccc gaaatatttc ctacgtgtat ccaaccgtcg tatgcgaaca tcgttgctag
    31561 caagcccgct cccaaggctg agaaaaatga aagattttct cctgcttgga tcggaaacag
    31621 ttggaagtct actccttctt gtcggaacaa accgaaaatg acgatcacaa acaaaggaat
    31681 aagcttgcag acaagcgtaa tcgactgaaa agcccctcca gctttagaac ctaaaaaatt
    31741 gatcaacagg atcgatacag cagctgtaac agctacggga acaatcattg actgggaaag
    31801 accaaataga ttgacgaact gtgtaccgaa aatgatcgac aaggctgcta catttgctgg
    31861 aaaataaatc acgacttgcg cccagcccaa taaaaaagct gctgtatttc cgtaaatccg
    31921 ttcgatatat ttaatcatcc ctcctgtttc tggaatagca gctgccagtt cagctcctgt
    31981 tagtcctgca cagacagaaa tcataccacc gagaaaccaa gaaaacatat gcaaactggc
    32041 ggatcctgta acttctgcta cgcttgcggc tttaaaaaat acccctgcac cgatcactgt
    32101 gcccataaca gtcgataaag cagtaaaaaa tcccatcgta cgttttaatt cattttctat
    32161 ttttacttct tttactggcg tggcttgata ttcttccatc ccttgtcccc tttcatacca
    32221 tagccaatcc caaaagtata aaaaactttt tggatgtggt tcttgtttta taccgtctct
    32281 accaattttt ctttcagttt ttccaacata tcctttgtca tcacatctaa gtcgtatttc
    32341 ggcgaaaagc cccattcctc ttgcgcacaa gaaacatcta gtgaatttgg ccaagattct
    32401 gctattgact gacgtacagg atccacatca taatccattt caaaatctgg tatgattttt
    32461 tgaatagatg ctttgatctc ttccgggtca aaagacatcg ccgatatatt gaaggcattt
    32521 cgatgaacca atcgatcagg atctgcattc atgagtttta taatggcatc gatcgcatct
    32581 ggcatataca tcatatccat tgatgtacct tgatcaatga aactagtgta ctttccttct
    32641 ttgattgctg aataataaat atcgactgcg tagtctgtcg taccgcctcc tggcaaggtt
    32701 gcataagaaa tcaatccagg gaaacggaca ccacgagtat ctacgccata tttcacataa
    32761 taataatcac atagtagttc tccagctacc ttcgttaccc catacatggt tgtaggacgc
    32821 tgaagagtat cttgtggggt atgatctttt ggagtatttg gaccaaacgc gccgattgaa
    32881 cttggcgtaa aaaatttcag ctggaattct ctagcgactt ccaatgcatt gaccagccca
    32941 gtcacgttca aattccaggc taattgtggt tttgcttctg caacagctga caaaagagca
    33001 gccaaatgaa tcaatgtgtc cacttgatat tgttctgcta tgctgcgcat tttttcgtaa
    33061 tccattacat ctaggatctc aaagatatcg tttttctcaa tgacttcagg catgcgtata
    33121 tctgtcgcaa tgacattttc actgcctagt tcttgacgta gccgtgtgat caactctgaa
    33181 ccaatttgtc ctaaacatcc tgtcaccaat acttttttca tgatgatcct ccctgatctt
    33241 ttttactact atcttcagat gattaacaat atatgcgtga acatgttctc taattttaaa
    33301 tagacgaaaa aactaaattt agttaaataa tttccatttc ttttcctatt ttttgataaa
    33361 ctgctactgc ttcatcaagc atttccctcg tatgttccgc agtcggcata tttctgatcc
    33421 tgcctgtccc aagaggcaca gttggataaa cgattggttt agcataaatc ccatgttcga
    33481 tcagcttctt agagaaagtc tgagtcactt tttcgtctcc caaaatgact ggagtaatag
    33541 gtgtttcaga cataccgatg ttatagccta cttttttcaa ttcttcttta aaataattag
    33601 cattttccca aactttttcg acaagttccg gatgttcttg catcaaagta atcgaagcca
    33661 atgcagctgc agctgcacct ggagttagcg aagtagaaaa taggaatggc cgacttctag
    33721 ctttcaacca gtcgattagt gttttcgatc cagctacata acccccaaca acacctatag
    33781 ctttcgataa cgtgcccatt tgcatatcga ttttgtcgct taatccaaaa tgcttgacgg
    33841 ttcctgcacc ttttccagtt acgcctgagc cgtgcgcatc atcgacgtag gtgatcagtc
    33901 catatttttc aacaatcgga ataatttccg gcaatcgtgc aatgtctcca tccatggaga
    33961 aaacaccatc tgtgatatac atgatttttt tatatttctt actttctatc gcttcctttg
    34021 cttttttctc aaggtctttc atatcttgat gtttaattcg gatgattttt gctccggata
    34081 agcggcaacc gtcaataata gatgcatggt ttaattcatc cgataagata gcatcttctt
    34141 ttgtcattac tgcagagata gcgcccatat tgcagttgaa tccagattga aaggcgattg
    34201 ctgcttctgt ccctttgaat tctgcgattt tttcttctaa ttgctgatgg attttcagcg
    34261 aaccattgat cgttcggact gctcctgctc ctacaccata agtttcggtt gcttcaatac
    34321 atgctttttt caattcttcg cgattggcaa atccaagata attgttggat gctagattga
    34381 tcatcttttt tccgtctacg atgatacaag caccattttc actttctagt atgtcgatcg
    34441 tattgtataa ccccttttct ttcagttgat ccaattcttt ctctaaatac attgttagtt
    34501 ctttggaaga cattgtctcc acctccatat tcatctgtct tgcagaacat accaaacata
    34561 tatcgtgtca ttcatctaca aagcaagtat gctatatttt tgataacgct tacactgatt
    34621 atgagaatta tttaaccaca ttttttattt acagtatctt tttactttca aatgacttat
    34681 atagtatgaa caattacctt ttagataatg atctatttta ctctacctgg gcagataaat
    34741 acaacacaac cattgataga agccctttcg ttaaaatcaa ttgtattctg aatatttaat
    34801 ttatttattg catagaccaa taaaaataca taaaatttcc atcttcgttt atcagcaatt
    34861 tttaatttta ctctctgaat atttcttaaa agttttactt gttgaattcg aaaaaaggac
    34921 tgtgacaaaa gtcttgtcac agtcctattc tt
//
LOCUS       DBNICI010000009        34560 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.009, whole genome shotgun sequence.
ACCESSION   DBNICI010000009 DBNICI010000000
VERSION     DBNICI010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 34560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.009"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(129..585)
                     /locus_tag="AC1ZCC_000382"
                     /pseudo
     CDS             complement(129..585)
                     /locus_tag="AC1ZCC_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            750..3112
                     /locus_tag="AC1ZCC_000383"
                     /pseudo
     CDS             750..3112
                     /locus_tag="AC1ZCC_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            3480..4403
                     /gene="truB"
                     /locus_tag="AC1ZCC_000384"
     CDS             3480..4403
                     /gene="truB"
                     /locus_tag="AC1ZCC_000384"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIQ1628668.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4407..5348
                     /gene="ribF"
                     /locus_tag="AC1ZCC_000385"
     CDS             4407..5348
                     /gene="ribF"
                     /locus_tag="AC1ZCC_000385"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HIQ1628669.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5700..6485
                     /locus_tag="AC1ZCC_000386"
     CDS             5700..6485
                     /locus_tag="AC1ZCC_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIQ1628670.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6498..7349
                     /locus_tag="AC1ZCC_000387"
     CDS             6498..7349
                     /locus_tag="AC1ZCC_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HIQ1628671.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7473..7925)
                     /locus_tag="AC1ZCC_000388"
     CDS             complement(7473..7925)
                     /locus_tag="AC1ZCC_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1628672.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8390..8758)
                     /locus_tag="AC1ZCC_000389"
     CDS             complement(8390..8758)
                     /locus_tag="AC1ZCC_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HIQ1628673.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8908..9390
                     /locus_tag="AC1ZCC_000390"
     CDS             8908..9390
                     /locus_tag="AC1ZCC_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HIQ1628674.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9427..10437
                     /locus_tag="AC1ZCC_000391"
     CDS             9427..10437
                     /locus_tag="AC1ZCC_000391"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 family peptidase"
                     /protein_id="HIQ1628675.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(10552..11265)
                     /locus_tag="AC1ZCC_000392"
     CDS             complement(10552..11265)
                     /locus_tag="AC1ZCC_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628676.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(11455..12045)
                     /locus_tag="AC1ZCC_000393"
     CDS             complement(11455..12045)
                     /locus_tag="AC1ZCC_000393"
                     /EC_number="3.2.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
                     /protein_id="HIQ1628677.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            12529..12756
                     /locus_tag="AC1ZCC_000394"
     CDS             12529..12756
                     /locus_tag="AC1ZCC_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HIQ1628678.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            12772..13938
                     /gene="hemW"
                     /locus_tag="AC1ZCC_000395"
     CDS             12772..13938
                     /gene="hemW"
                     /locus_tag="AC1ZCC_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIQ1628679.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKNGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            14172..15215
                     /gene="hrcA"
                     /locus_tag="AC1ZCC_000396"
     CDS             14172..15215
                     /gene="hrcA"
                     /locus_tag="AC1ZCC_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIQ1628680.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            15309..15872
                     /gene="grpE"
                     /locus_tag="AC1ZCC_000397"
     CDS             15309..15872
                     /gene="grpE"
                     /locus_tag="AC1ZCC_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIQ1628681.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            15923..17752
                     /gene="dnaK"
                     /locus_tag="AC1ZCC_000398"
     CDS             15923..17752
                     /gene="dnaK"
                     /locus_tag="AC1ZCC_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIQ1628682.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            17903..19069
                     /gene="dnaJ"
                     /locus_tag="AC1ZCC_000399"
     CDS             17903..19069
                     /gene="dnaJ"
                     /locus_tag="AC1ZCC_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIQ1628683.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(19437..20174)
                     /locus_tag="AC1ZCC_000400"
     CDS             complement(19437..20174)
                     /locus_tag="AC1ZCC_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIQ1628684.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(20176..21678)
                     /locus_tag="AC1ZCC_000401"
     CDS             complement(20176..21678)
                     /locus_tag="AC1ZCC_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HIQ1628685.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            21937..23931
                     /gene="uvrB"
                     /locus_tag="AC1ZCC_000402"
     CDS             21937..23931
                     /gene="uvrB"
                     /locus_tag="AC1ZCC_000402"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIQ1628686.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            23941..26757
                     /gene="uvrA"
                     /locus_tag="AC1ZCC_000403"
     CDS             23941..26757
                     /gene="uvrA"
                     /locus_tag="AC1ZCC_000403"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIQ1628687.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            27020..28285
                     /locus_tag="AC1ZCC_000404"
     CDS             27020..28285
                     /locus_tag="AC1ZCC_000404"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HIQ1628688.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            28289..29020
                     /locus_tag="AC1ZCC_000405"
     CDS             28289..29020
                     /locus_tag="AC1ZCC_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HIQ1628689.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            29155..30039
                     /gene="rapZ"
                     /locus_tag="AC1ZCC_000406"
     CDS             29155..30039
                     /gene="rapZ"
                     /locus_tag="AC1ZCC_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HIQ1628690.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            30036..31034
                     /locus_tag="AC1ZCC_000407"
     CDS             30036..31034
                     /locus_tag="AC1ZCC_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HIQ1628691.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            31068..32003
                     /gene="whiA"
                     /locus_tag="AC1ZCC_000408"
     CDS             31068..32003
                     /gene="whiA"
                     /locus_tag="AC1ZCC_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HIQ1628692.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            32571..33227
                     /locus_tag="AC1ZCC_000409"
     CDS             32571..33227
                     /locus_tag="AC1ZCC_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628693.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            33406..33971
                     /locus_tag="AC1ZCC_000410"
                     /pseudo
     CDS             33406..33971
                     /locus_tag="AC1ZCC_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326931.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        11513 a         5825 c         7370 g         9852 t
ORIGIN      
        1 caggggttgc gggatttttt tgttttcatt aagacgtatt acctaagagt tccatgactt
       61 tagtcttgcg gcgtgtcagc taaccaaaaa agagaaaaag aaaagtaaaa aattcgactt
      121 tcttttttct agtagtatat aaatttttgc atatagataa agatcaagat accgataacg
      181 gaagctagat tgtaatctat tttagtgaca gtcaggtata gccaagtaac tagaagatca
      241 gtggaacctg caccataaaa gtttgagttt gtcggtccgt tgacagttaa aagaaaaatc
      301 acgttgaagt tattgatatt tccgattacg ccgcttcgat ttggtcacga tccaaatttt
      361 ggataatccc tgtagaaatt agcatagtag ctgggatacc aattcacata ttgacaaaga
      421 taacagagac ttttacataa aacggatcaa tcaggaaagg aattgctcga tccagcccca
      481 gttttccagc aatgtgttaa ctggcccagc gtcattcagc aaattatgca tgatgaggag
      541 cgaaatgaac tgcggaacag ccattgtgat aacgacaagt tttttggatc aatgtaaaaa
      601 acgtcagcga aaggaaaaat ttcgtggttt tttatacctg ttcatttggc tagatataaa
      661 atccaaacaa atgaacagaa gtgcttatta ggtttcaatt gggagtatct gcgctagaat
      721 aagagtaata atgaaaagga gtgttatgta tggattattc tagtaaagaa tactttgata
      781 aaatgacggc ttggtggcgt gctgctaatt atctttctgt tgggcaactt tatcttaaag
      841 ataacccctt actgagacgt acactgaaac ccgaagatgt caaaaaacat ccaatcggtc
      901 attggggaac gattcctggc caaaatttta tttatgtcca tttgaaccga gtcatcaata
      961 aatatgattt gaacatgttt tatattgaag ggccaggaca cggcggacaa gtcatggtat
     1021 cgaatgctta tttagatgga agttacacag agatctatcc agaagtaacg gaagatgaga
     1081 caggaatgca aaaattattc aaacgatttt catttcctgg aggcattgct tctcatgcag
     1141 caccagaaac accaggttct atccatgaag gcggagagct tggatactct ctttcacatg
     1201 cagtcggagc tgtattagac aatcctgaag taatctctgc tgtcgtgatt ggtgatggtg
     1261 aagcagaaac cggtccttta gctggaagct ggttctcaaa tgtctttatc aatccagtga
     1321 tagatggagc tgttttaccg atccttcatt taaatggtgc aaaaatagcc aacccaacga
     1381 tcttggcacg taaatcagat ggagaattag caaattactt taacggactt ggctgggaac
     1441 ctttctttat tgaaggaaat gattcagaaa aattaaatcc tgttatggct gaaaaaatgg
     1501 atcaagcaat cgagaaaatt aaatccatcc aaaaagaagc tcgcctgaag acagcaacag
     1561 atgtcgtgat gccaaaatgg ccagtattga tcgtacgcac accaaaagga tggaccggtc
     1621 caaaagaatg ggacggggaa ccaattgaag gaaccttccg agcacaccaa gtaccgattc
     1681 ctgtagatca agaacatatg gatcatgcag atgctttact ccgttggctg aaatcctatg
     1741 aaccagaaaa attatttgat gcacaaggac gtattttaga agaaatcaga gaaatcgcgc
     1801 ctactggtga ccaaagaatg gcaaaaaatc ctatcaccaa tggtggaatc gatccaagac
     1861 cgcttattat gccagattgg aaaaaatata ctttgcaatt tgaaaaacca ggctccgtta
     1921 aagcagaaga tatgaccgaa cttggtaaat tcgttcgcga gatcattgag aaaaatccag
     1981 aaaatttccg tatatttgga ccagatgaaa caaaatcaaa ccgattgaat caagttttca
     2041 aaacgacaaa tcgccaatgg atggaaaaaa tcgaaccaga aaatgatgaa tggctttcgc
     2101 catctggtcg cgtcatcgac agtcaattat cggaacacca agatgaaggg ttcttagaag
     2161 ggtacgttct gactggacgg catggttttt tgcaagttac gaatcattct tgcgagtggt
     2221 tgactctatg ttgactcagc acttcaaatg gatgcggaaa tcacatgacc tttcttggcg
     2281 aaatgattat ccttcgctaa atctcattgc aagctcaaca gtcttccagc aagatcacaa
     2341 tggatatagc caccaagatc caggaatatt gacacatttg gcagaaaaga aggcggagtt
     2401 catccgtgaa tatctgcctg cggatgcaaa tacattgctc gcagtcatgg ataaagcatt
     2461 ccgctcgtca gaaaaaatca atttgatcat ctcttccaaa catccgcgtg ctcaatttta
     2521 ctcagcagaa gaagcagccg tcttagtgaa tgaaggcttg aaaatcattg attgggcttc
     2581 gacagcaaaa gaggaggaac ctgaattggt cattgctgct gcaggaacgg aatctaactt
     2641 agaagcatta gcagctgtaa ctttgctgct ggaagaattc ccaaaactaa aaattcggtt
     2701 catcaatgtc gtagatttat tgaaattacg tcatccaagt caagatccgc gtgggttgag
     2761 cgatgaagaa tttgatcaat attttacaaa agacaaaccg atcttatttg ctttccacgg
     2821 ttatgaaaca ttggtccgca cgattttctt tgatcgtcat aaccatcatt taatgattca
     2881 tggctacaaa gaaaatggag atatcacgac accatttgac atgcgtgtag taaatgaact
     2941 tgatcgttat catttggcaa aagacgcagc tttaaaaatc aaaggaagtc aagcagaaga
     3001 ttttgcgaaa aaaatggatc agaaattaca agaacatcaa aactatattc gtgaaaatgg
     3061 tattgatctg ccagaagtat tggattggaa atggaaaaat cttgaccaat aaaatgataa
     3121 aatcgatccc gcaaaataga attttttaca ttgattaaga aactgaaaaa ataaataaaa
     3181 aaaacggaaa aaagactgga agaatggcca gtcttttttt tcgtttttgg gaagtgaaaa
     3241 ccaaactatt tacctgtaag ccacccataa atccaatcaa atggtgcaaa gaaaaaatct
     3301 tccattctat tccctccttt aaaaaagcat aacatgattt ctatttacgt gagcatgaat
     3361 tttgttacgt ttttattgcc gaaaaataaa aaccgttcat tatgatgtca aattcgtcag
     3421 taattgatgc aatgtgttat aataattcag ttaaatcaaa aatgaaatgg agaaaagaca
     3481 tggacgggtt attaccttta tggaaagaac gcggaatgac aagtcatgat tgcgtattca
     3541 aattaagaaa aatcttacat acgaaaaaaa tcggacatgg gggtacgctt gacccagacg
     3601 tagacggtgt gttgcccatt tgtattggaa aagccacaaa agttatcgaa tatttgacag
     3661 attctgggaa aatctatgta ggcgagataa ctctaggttt cagtacaacg acggaagatc
     3721 gatccggaga acgggtagaa acaaaaccag tagatatgtc tttatctgaa aaaaaaatag
     3781 atgaagtgat ggcttcattt attggtgaga tcacccagat accaccaatg tactcagcgg
     3841 taaaagtcaa cggtaagcgg ttatatgaat atgcccgcaa taatgaaaca gtggagcgtc
     3901 ctgttagaaa ggctcagatt taccgtttta tccggacaag tgaattgaca agagacgaag
     3961 tgacaggaac catttcttgg cgcttcgaag tagaatgcgg gaaaggaacc tatgtccgca
     4021 cattagctgt agatacaggc aagaaacttg gttaccctgc gcacatgtct gatctgaccc
     4081 gaacagcaag tgccggtatg tcccagaagg atgctctgac attagaagaa gtagctcaag
     4141 ctgtgaaaaa tgggacaatc gatgattgtt tgctgccaat agaaaaagga gtggaacggt
     4201 tcaaacgagt agatattact gatgaagtat ggcagaaagt aaaaaacggt atgagacttg
     4261 attatactgt atttgcgctc ccagaaatgc cgtcagaaga aattgctttg ttttataaag
     4321 gaaaagttgt cagtatttat cagccaaacc cgaaagaaaa aaacaaatta aaacctagta
     4381 aagtattgag aaatgaggaa taacgaatga atatcatcaa gatcagacat ccttaccaat
     4441 cagaacaaat tccagaagac gatgtcgttt tagtgctggg atttttcgac ggcgtgcata
     4501 tcggacacca gaaagtcata aagacaggta tggaaattgc acaaaaagaa ggattgaaat
     4561 tagcattgat gactttcaat cagcatccat ctattgtctt taaaaagatc gatcctagca
     4621 gcgtgaaata tttgactagc cttaagcaaa aagaagaaaa aatggccgct ttgggtatag
     4681 attatttata tgaagttgat tttacttctt cttttgctca tttagcaccg caggaatttg
     4741 ttgatcaata catcgtaggg ttacatgcaa aatatgctgt ttccggtttt gattatacgt
     4801 atggaccaaa agacattgca gatgtcgctc attttcctga atatgccaaa ggaagattca
     4861 aaatcgtcac agtttctaaa gaagaagaac aaggtgaaaa aatcagctct acccgtattc
     4921 gtggattact agacagcgga caagtaaaag aagcagcaaa actattaggt tgcacatatg
     4981 aggtagaagg ggtagttgtc catggcgatg cacgaggcag actactagga ttccctacag
     5041 caaatgtcaa agtaaagagc acggttcatt tgcctaaaga aggtgtttat gtgtcggaaa
     5101 taaaaattgg ggaaacatgg tatccagcca tgggttcaat tggacataat gatacattcg
     5161 gtgaaggacg ggagctgact gtagagttgt atattttgga ctttaatcaa gatatctatg
     5221 gtgaacatgt cgcggtacgc tggaatcaat ttttaagaga tcaagtgaaa ttttctagtg
     5281 tagaggaatt gattgaacaa ctcaaggagg atgaacaagc aacagctgaa tatttttctc
     5341 agcaataaaa cattatgcat aaaatctctt gtttatgtat aactagcaat tacaataaaa
     5401 tgggtgataa ataagcccag gaaaggggct gtgacaaaag tcttgtcaca gccttttttt
     5461 ccaaataaat ggtttttcag aagtagcttc ttcggaaata agccgaaatt tcttgaagct
     5521 gaccacttct gtcacaacct ctttctttat agaaataaaa ggacactagc aattcatgaa
     5581 aacgattgct agagcgtatc atttgcttta gactatatat cttggtgtac actcacttta
     5641 aggcggaaat aaaagttatc cgtaataaca ggtttaggta tgaaaaagga gtgggtacta
     5701 tgtacaagga attagaaaac aaagtcgcag tagtaactgg tggctcaaag gggattggaa
     5761 cagcgattgc tcaacgattc gggaaagaaa aaatgaatgt agttgtcaac tataactcgg
     5821 ataaagaagg agcagaaaaa gcagtcaaag caatcgaaga ggcaggcgga aaagcagtct
     5881 ctgtacaagc agatgtcagt tctgaagaag gaatacagcg tttacttgat acggctttag
     5941 aaaattttgg gacactggac gtttgggtta ataatgctgg gatggaaaat cagcatccaa
     6001 cccatgaact tagccttgaa gattgggaaa gagtgttaaa agtcaatttg actggcgttt
     6061 ttatcggaac gaaagcggct ttaaattact ttacagagaa taataaaaaa ggaaatatca
     6121 tcaatatgtc ttctgttcat gaacagatcc catggccaac gtttgctcat tacgctgctt
     6181 caaaaggcgg ggtaaaatta tttaccgaaa cggtagcaat ggagtatgca ccgaagggaa
     6241 tcagaatcaa tagtataggc cctggtgcta tccgtacacc tattaatgcg gcaaaatttg
     6301 atgatcctga acaaaaagaa ctcttggaaa gtatgatccc actaggacgc gtaggtgaac
     6361 caaaagaaat agcggcgaca gctgcatggt tggcttcaga cgaatccagt tatgttacag
     6421 gtattacact atttgcagat ggtgggatga ctttatatcc ttctttccaa ggtggaaaag
     6481 gataagggtg aataaaaatg ggtttattga ttgcattgat cccggcagtt ggctggggga
     6541 tccagccact gattttgaaa aagattggtg gcagaccaac aaatgagata ttaggaactg
     6601 gtattggagc ggtgcttgtc ggcttactcg ttcaattatt cgcttctcca gctggtattt
     6661 cagtgactac atttttgatt agtttacttt ctggtgcatt ttgggttatt ggacaaacag
     6721 gacagtatac atctttcaac ttgattggtg tttctaaaac gatgccaatt tcgactgcat
     6781 tacagttagt cggaacctcg ttgattggtg tcttcgcttt tggagaatgg tctggaagta
     6841 tggggaaaat cattggagca gcagcaatca ttcttcttgt atttggttct gctttaacgg
     6901 caatcagtga tgatcctgga aaaaaaggcg gtatcacaaa aggaattagt ctattagcat
     6961 caacaagtat tggttattgg gtatacagtg cattgcctaa actagttgat gcaagcggac
     7021 tttccatttt ctttccgcaa atgatcggtg tctttttagg ggcagtgatt tatgtgattt
     7081 tccgtaaacc aaccgcattt tctgaagaca agagttggaa agcaacgatc gttggagcca
     7141 tctttagtgt gtcagcattg gcttatattt tttcagcaaa tgaaaatggg gttgctactg
     7201 catatatcat tacacagcta aacgtagtta tctctacatt aggcggtatg tacatcctgc
     7261 atgaaaataa gacaccaaaa gaaaaaaaat taacgattat cggattattg ctgatcgtag
     7321 taggaagcat ggtcacggtc tttatttgag gataaagatg gtaaaatttc atttattaaa
     7381 aaaatatttg tttgaaccaa acagtaagta cttataaaaa aatagcgcta gaatttccaa
     7441 catctctgga atttctagcg ctttttaatt gtttaagata caagggaaat atctgtctgg
     7501 atcaataatt tttcattgat ccattgacta aagtttacga cataattgaa gttaccaccg
     7561 attagtatct ccgcatgatt gtctttataa agatcgtatt gtaaaagacg gtctttttta
     7621 tttgcgtaaa catcgttatg gtactggact aattgttctc ggaattgatt gattgctgta
     7681 aaatcacgat attgaaggtt gaaacgcatc gtgattggtg catttagcat gaatcgctgc
     7741 atttcttttg ctgcttcctc tgcttgatct ccgtatagga actcaaaaag attacgttca
     7801 gaaaatttcc attgcccgcc aacctttgtt ccttttagtt ttccttcatg aagataattt
     7861 ctgatagaac gtgtagtaac acctaattgt tcggctaatt catttactga gtaaaaatct
     7921 ttcaatgttc accctcctat gtgaatggtt ctactgactt agttgatatt tgcttaatca
     7981 cgaaatgtag aaagaacgat tttaattaag gtcattgtaa aaatcaagtc agaaaattct
     8041 aacttttatt tctctaaaga aattcgttct ctcctaaagt gatattatat ttaaatatac
     8101 ctgaaaataa tgggcagtat tatgaaagat cactgatatt ttctcgtatt ttttccttat
     8161 tcaaaagttt tgcaggaaaa ttgctgaaaa aataatttta ctaagtagaa aattcaaagc
     8221 cattcaatca gtgatttttt tcagcacaag aaagactcct tataaaaata gcagatactt
     8281 tgtcaattaa gtgtttaacc agggatgata aaaaggatag gattgaacaa aacctgtata
     8341 aggtgatgaa tagtttcctc gccagtaaaa ggtttattcg tataaagctt tatcgccaaa
     8401 atttatcatg acctttttta tcttcaatat taaatgaaat caattgttca atcaattccc
     8461 agttgattac acttttccaa ggaactttga tgacgttatc cgtatatacg tagcctgctt
     8521 cttggatatt tttttcaagg gcacgaatcg ctgctttttc cggtgcgata gaaaaatgat
     8581 ttttagcaaa actaaatgca ataataaagg taccatggtc agtaaacata ggttggttcc
     8641 attttacagt tgtgtctagc tggggatacg tttctgttat cttagcaaaa aggtctttga
     8701 tcttttgttg atgttctttt tctttgattt gattgatgat cggctgaaat ttttccattc
     8761 taaaacgcct ccattaagtg gtttgattta ttttaccaga aaaacaatag ttatgtataa
     8821 aaaacgaaca aaacacaagt aatgttgtaa ccgctatatt tatatgataa gatgtctcaa
     8881 ataataaaat agaaaagaga cataaaaatg attgacttgg caaagaaatt cttacacgaa
     8941 aatatatatt ttgagttgta tcatcatcga gccatttata caaatgaaga tgctttggta
     9001 ataaagaaag aacaaggatt tacaggtaca gagaccaaaa gcttattctt aaaagataaa
     9061 aaagataata actacgtata tctgactttt actacgaaac aaacggattt caaagcatta
     9121 gggaagatcg tcggtaaaag gctttctatt gtttcatcag aacgcatgga agaacaaacg
     9181 ggacaaaagc agggcgctgt ttctccgttt ggttatgaaa aacagattcc gattattatt
     9241 gatgaagagc tcttatcaaa tgaaaaactt gtttttgcac caggtagacc agatcaaacg
     9301 atggtcatcc aaacggttga cttaaacaaa atcatccagc tacttgagat gccgatattt
     9361 ttgatgccgg caaacgaaaa aaacaattaa ccaagtaaaa tagtgcatac ttggaggtga
     9421 agcaacatga taagttatcc attattagaa agaaatgcaa cgattggggt tactgcacca
     9481 tcctcaggcg tatcagagga acaccattcg ttactcgaat cagctttaaa gagaatggaa
     9541 gaaaaatctt tccaagtagt tactggaaag acgctttgga cgcaagataa ggaaaaatca
     9601 ggtccagcaa gaaaacgagc agatgaattc aatcaaatga tgcaagatga aaatatccaa
     9661 atgatctttc cgacttgggg aggagaatta ttgattgaga ttctcgattt tgtcgatttt
     9721 gatgctctac aaccaaaatg gatagtaggt tattcagata ttagtacgtt gctacttgct
     9781 gtcacattaa aaacagggat agccactgca catggaacta accttattga tttaagagga
     9841 gagtacttgg atgaaacgac agcaatgtgg gaaagtgtac tgaaaacaag agaaggagaa
     9901 tcgattctac agcaatcttc tgaaaagtat caaaaagtgt ggccccatga gcattcatca
     9961 tcttctgttt tccatctcac tgaaccaaca aattggcaat cagtcagcgg agatgcagta
    10021 aggatgaagg gacgattatt aggtggttgt attgatacga tcaggcattt aatcggtact
    10081 ccttatggag atgtccaatc ttttaaaaga aagtataatg atggaaagcc gatcttgtgg
    10141 tttctagaaa actgtgagct atcgtcagtt gatttaagaa gatcacttgt tcagatgaaa
    10201 ctggcaggct ggtttgatgg cagctcgggg atcatgttcg gtagaagtgc ggcagaggat
    10261 ttgttagatt ataaaatgaa ggatgtatac gaagatctgt caaaggaatt aaaactaccg
    10321 atcttgtatg atattgattg tggtcatgta ccgccacaac tgacattgat taatggtgcc
    10381 tatgcagaag tagcggtaga agatagaaaa gcgactgttt tgcaaacgtt tatttaaaaa
    10441 attttttcct gccttggagt ctatcaaaga ccaataaatc taaatagaaa agaaagtgaa
    10501 agaagctgac aaaatgctgt cagcttcttt ttgtataaca tattagaaag tctaattatc
    10561 tataactgtc acatcgatta actgtttcac gtcattggga tttcctgtag gaatagctac
    10621 tacagatgca ttatcaatga tttcttgagt tgtttctcta atgaacgctg gataagatgt
    10681 ttgatctgca ggcatttgcc aatgtgaagg aactggataa ctagtgattg tcccgtcatg
    10741 gttacttgaa tacactaaaa ctccctggta actgtatttc ccagtgagca taaccacatc
    10801 tttcggatag actgcactcc ctgcatcata aggggcgata ggtgttcctg caggataatg
    10861 aataacatgt aattcgaaat cacccgatgt atctctaaaa gtattacctc taatagctag
    10921 ccaaacacgt gcgtactcaa tttgtagatc agaatatcct gctaacggat ctattgactg
    10981 agaagactgt gaggaggttg tgtctattgt tgaagaagaa atatttgtct caactgtact
    11041 actagatgta gaagaagtat tgggctctga gactttactg cttgttgaag atgtattcgt
    11101 ttcagtagaa gctgatttct gagtacttga tttttcagct gaactggttt gtctgattga
    11161 acttttgtct gtggtcgcag atggatcact attttcttta ctaggagacg tacatcctac
    11221 aaggaataaa gagaccaaca ataaaataaa acctaacttt ttcatataaa atacctcatt
    11281 atttttttaa taccagtata gcataatcct gaatttttga atccgtaaaa ccttgtctat
    11341 gaatataaat agagtgtatc ttctttgata aatgtcagaa aaagtgtgac aaataatcat
    11401 cagtaaaatt acttgtcaca ctattgataa ggagtattta attgaagaga atgattagga
    11461 gaaaggatct ccgtactttt tatagtaatc atagaatcca agtttgtata gacgaatcgt
    11521 tttttctctt atttcttctg tttcaaaatg gactgcttga agaatcaagt ctgtgaattc
    11581 tataggagct gttccattcg cagtaatcaa attacgatct ttcacagctt gttgttctat
    11641 aaaatcttct ttattctgat aattttccga ttctaaccaa ggaaattgcg catttccggt
    11701 gtgtttgtaa ccttgcaata atccgctctg agctaaataa tcaactgccc cacaaatagc
    11761 agctactggt acgtctgttt ttaacaattt atcgattatt tgatataaat gatgattttt
    11821 tatgtcccat gaattgccac cgatcataat aaaaaggtca actttttcag gtatttgttc
    11881 gattaagtaa tcgatttttg tttttaatcc tccaatggaa gtgacctctg attggataga
    11941 agcagttttg ttttgccatt tctcattttg gtttagtgtg ctagtaatat aagcaccttc
    12001 ccaatcggca tattcctcta atagaaacgt aatcgcagtt ttcattttca agctctcctt
    12061 tactatctat aacaaggaca acagttttaa cctacagaca ttcattaaaa ttgggtagcg
    12121 acagcgaaaa caacaattct taagttttag acagagtata cagatagtca acttaattaa
    12181 atacgaacta aataaaaatg acaggtaatc atttttgttt accagcatta taacattttg
    12241 catacatgga tgacaaaaga accaattccc acttgcttga gtacaatatt aaaagaaact
    12301 ggaaggcaaa aagtaattat agaaacgaaa aataaatcat atcgggcact cagacatgaa
    12361 aacaacccta gagatatata actaggtaac cagtttaatg aaagaaaaaa tcatccaaga
    12421 agtcgattct tggatttttt agtggatttg ttcgccaaat acgtttagta tgcgtatatg
    12481 agaggttgta agcaaagcgt tgtggtctga gcacttaagg aggaatctgt gaaaatagct
    12541 tttcatattt ttgcagattt tgacttaatg ccgaaggtca gctttgcgat taccgtttat
    12601 cgagaggctg taaaagcacc gtgtagctcc gatgacaata agcaagcatt tttagagcta
    12661 ggtttccttg attctaaata tgcggcttat ttcaatgagt tggtgcttga acgcgatttt
    12721 aagaaggtcg aaaacaggca cgacttttcg acgtaaggag aaagaatttc aatgatagaa
    12781 acaataaatc ccgcagaaaa caaggtttct gcgtacattc atatcccatt ttgcgagcat
    12841 atctgctatt actgtgattt taataaagtt tttttagaag ggcagccagt agatgagtat
    12901 gtggaaatgc tgctgaatga aatgaaaata atgctagaaa gtcatccgac agcgaattta
    12961 gaaacacttt acgtaggggg cgggacacca acttctttat ctgctaaaca acttgatcga
    13021 ctgttatcag gtgctagaga gatcctgcct tttcaaaatg gtaatgagtt tactgtagaa
    13081 gcgaatccag gtgatttgac ccgtgaaaaa cttcaagtaa tgaaaaacta tggcgtcaat
    13141 cgtttatcca tgggagtgca gacatttgat aatcgcttat tgaaaaaaaa tggacgtaaa
    13201 cacaccgcag aagatgtgta tcaaactatg caatttttag aagcagaaaa ttttaccaat
    13261 gtaagcatcg atttgattta tgcattaccg ggacaaacac tagaaggata tctggatact
    13321 ttggaaaaag ctttggcttt ggatctgcct cactattctt tgtactcatt gatcctcgaa
    13381 aacaaaacaa tgttcatgaa ctgggtacga caaggaagac tcgaactgcc tgatcaggaa
    13441 acggaaacca gaatgtttga agagacgatt gaagcaatgg aaaagcatgg acgtcaccaa
    13501 tatgaaataa gtaactttgg gctctccgga catgaatctc aacataacct catgtactgg
    13561 aacaatgccc actattttgg gtttggagct ggtgctagcg gttatcttgg agatattcgt
    13621 taccgcaaca aaggaccaat ccaacactat ttacgcccat tacgagcaaa tgaacgcccg
    13681 gtattggaag aagaaaaatt gacacgaaaa aatcaaatag aagaagaaat gttcttagga
    13741 ctaagaaaaa aaacagggat tactaaaaaa catttctatg atcgctatca aaaaacaatt
    13801 gaatcggtgt acggtgatgt gctaagggaa ttagagcaag aagggttact tgttaatgaa
    13861 gaagaccgtg tttatttgac accaagaggg acctttttgg ggaacaatgt gtttgagcgc
    13921 tttttgtttg aaaaatagtt taaagaatat ttgtttttta tctgaattta aataaaaaaa
    13981 gttcaaaaat acaataaagg actattgaaa cgttgatata actggattta catcaattat
    14041 ttagaattag cactcttgtg tttagagtgc taaaaaatgg agtctttctc ttgacaggag
    14101 aagggcttct tgctatattt ataagtggat tagcacttag aatatatgag tgctaatgag
    14161 aggtgaggaa gatgttaaca aagcgacaaa acgacatttt acgtctgatc atccaacact
    14221 atacgagtag tggcgtgccc gttggttcca aaacattgat gtctgaggga gtcaaggcca
    14281 gctcagcaac gattcgcaat gatatgaaag cattagaaga tgaaggattg ctgttgaaga
    14341 ctcactcttc ttctgggcgg attccatctg ctttaggcta tcgttattac gtggatcatt
    14401 tgttaaaacc tgctcgcgta gcagaagatg atttgctgct gattcgtcag tctctaggca
    14461 gagaatttca tgaaatcaat gagattatta agcaatctgc tgagatatta tctgagctga
    14521 caagctatac gactttttca ttgggacctg aaataaaaga ccgccgactg actggctttc
    14581 gtatcgtacc cttgaataat cgacagatta ttgctatcgt cgtaacagat atggggaatg
    14641 tagaaagcca agtgttcact cttcccgaat atatgggaag tcaggaacta gaaaaaatgg
    14701 tacagattgt aaacgaccgt ctagtcggag atcctctgat gacggtgtac cataaacttc
    14761 gtacggagat tccgatgatt cttcataaat atttccaaac gacagaaggg atgtctcagt
    14821 tatttgacac cgttcttggt catgtttttg aagataaggt ttatgtcagt ggacaaatga
    14881 atctcttgga ttatgagccc cgtcaagatc ttgatcagtt caaatcgatg ttttctttta
    14941 tgaaagactc agacggactt acacaaatga tcactccgat ggatagcaat attcatatca
    15001 agattggtgc tgaattaggc aatgatttat tgcagaatat gagtatgatt caagcaagtt
    15061 acgagataga tggtcatgga agaggtacaa tagccttgtt agggcctact agtatgcctt
    15121 attctcggat gttcggtcta gtcgatgttt atcgaagaga actagcagct aaacttgctg
    15181 attactaccg ttcgcttgac ttgtcaaact cgtaatgtga ataacgaagc tatttgcagc
    15241 tgatcgttct ctctttctta gtaagagcaa tcaaacagct gactgaataa tgaaaggaag
    15301 actgtatcgt gaaagaaaat caagaagaac tagataaaga aatgacagat gctcagccag
    15361 aaccggaaat cgatgtcgaa gctgcagaag attcgggtat ttcagaagct gaagcggaag
    15421 aattcgaaac agcgaaatta aaagctgagt tagaagaaat ggaagataaa tacttgcggg
    15481 ctcgtgctga aatcgcaaat atggcaaatc gaggaaaaaa tgagcgtgag caattacaaa
    15541 aataccgttc acaagattta gccaaaaaat tacttccatc aattgacaac ctggaacgtg
    15601 cattagccac agaagtgtca gatgatcaag gggcaggttt gaaaaaaggc gtggaaatgg
    15661 tcttggaaag tttgcgtaac gcactggaag aagaaggaat tgaaaaaatc cctgctaaag
    15721 gagaagcctt tgatcctaat cttcatcaag cagtacaaac tgttccagcg actgaagata
    15781 caccagccga tacgattgta gaagtactac aagaaggcta caaattacat gatcgcgtgt
    15841 taagaccaac catggtcatt gttgcacaat aattgtgcgg atataaaact tacactttca
    15901 attaaagaag gagacgttaa ttatgagtaa aattatcggt attgacttag gtacgactaa
    15961 ctctgcagta gcagtattgg aaggaaacga agcaaaaatc atcgctaacc cagaaggaaa
    16021 ccgtacaaca ccttctgttg tatcattcaa aaatggagaa atccaagtag gggaagttgc
    16081 aaaacgtcaa gctgtgacaa acccaaatac gatttcatca atcaaacgcc atatcggtga
    16141 agcaggttac aaagtagatg tagaaggtaa atcatataca ccacaagaaa tctcagcaat
    16201 gatccttcaa tatatcaaag gattcgcaga agactattta ggtgaaaaag tcgaaaaagc
    16261 agttatcact gtgccagctt actttaatga tgcacaacgt caagcaacaa aagatgctgg
    16321 taagatcgct ggtctagaag tagaacgtat cgtcaatgaa ccaactgcag ctgctcttgc
    16381 ctatggttta gataaaacag atcgcgacga aaaaatctta gtattcgacc ttggtggcgg
    16441 tacttttgac gtatctatcc ttgaactagg agacggtgtc tttgatgtat tgtcaacagc
    16501 tggcgacaac catctaggtg gggacgactt tgataacaaa atcatcgact acatggtagc
    16561 agaattcaaa aaagaaaaca gcattgactt gtctcaagac aaaatggctt tacaacgctt
    16621 gaaagacgct gctgaaaaag cgaaaaaaga tttatctggc gtatcaagca cacaaatcag
    16681 cttgccattt atcacagcag gagaatctgg accattgcat ttggaaatga cattgacacg
    16741 tgcaaaattt gatgaattaa cagctgactt agtagaacgt acaaaagtac cagtacgtca
    16801 agcgttaaaa gatgcaggtc tgtcacaatc agaaatcgac gaagttattt tagtcggtgg
    16861 atctactcgt atccctgcag ttgtagaagc agtacgtaaa gaaacaggaa aagaaccaaa
    16921 caaatcagtt aaccctgatg aagtagttgc tatgggtgct gctatccaag gtggcgtgat
    16981 cactggtgat gtgaaagatg ttgttttact tgacgttaca ccattgtcat taggtatcga
    17041 aacaatgggt ggagtgttca ctaaattgat tgaccgcaat acaacgattc caacaagtaa
    17101 atcacaagta ttctcaactg cggcagataa tcaaccagca gtagacatcc atgtattaca
    17161 aggtgaacgc ccaatggctg ctgacaacaa aactctagga agattccaat tgacagatat
    17221 tccagcagct cctcgtggta ttccacaaat cgaagtaaca ttcgatattg ataaaaacgg
    17281 gattgtaaat gtatctgcga aagatctagg tacacaaaaa gaacaaaaaa tcacgatcaa
    17341 atcttcttca ggtctaacag acgaagaaat cgaacgtatg gtgaaagatg cagaagcaaa
    17401 cgctgaagca gataaagctc gtaaagagga agttgaccta cgtaatgatg tagacgcatt
    17461 gctattctct gtggacaaaa cattgaaaga attagaaggc aaagtggacg aagaagaagt
    17521 gaaaaaagca gaaacagctc gtgacgaatt aaaagccgct gttgaagcaa acaacatcga
    17581 ggaaatgaaa actaaacgcg atgcattgaa cgaaatcgtt caaaacttga ctgttaaact
    17641 atacgaacaa gctgcacaac aacaagctca agaaaatcca gaagcagctc aaggcggagc
    17701 agatgatgtc gttgatgctg attttgaaga agtcgatggc gacgataaat aatcagttca
    17761 ctgaatttcg agagaaggcc aaagttgcta ctttggcttt ttcttttgaa aaatagtttg
    17821 aagtatcggt ttttgtttca aacaaaatat gatatgataa caagcgacgt gaaaatgatg
    17881 agaaccaata ggaggaaagc ctatggcaac aaaacgtgat tattatgaag tcttagggct
    17941 ctcaaaagga gcatcagaag atgaaataaa gaaagcttat cgtaagcttt cgaaaaaata
    18001 ccatcctgac atcaataaag aacctgatgc ggaagaaaaa ttcaaagaag tatcagaagc
    18061 atatgagatt ttaagtgacc cgcaaaaacg cgctgcatac gaccaatacg ggcatgcagg
    18121 cacagatcca aattacggtg ctggtggagc tggtggcgga tttggtggat tcggcggctt
    18181 ctctggtggc ggcttcggtg gttttgaaga tatcttcgac tctttctttg gaggaggcgg
    18241 tcggtcagtc gatccaaatg cgcctcgtca aggtgctgat ttgcaatata cggtcgactt
    18301 gtcttttgaa gaagcgattt ttggtgtaga aaaagaaatc aagtataatc gggaagaaat
    18361 ctgtcatact tgtggtggaa acggtgcaaa accaggcaca caaccaacta cttgtcataa
    18421 atgtcatggt gcaggtacga tcaatgtaga acgccaaacg ccacttggac gagtgatgag
    18481 ccgtcaaact tgtgatgttt gtcatggcac agggaaagaa atcaaagaac cttgtccaac
    18541 ctgtcatggc tcaggacatg agaagaaagc acattcagtt aaagtgaatg taccagctgg
    18601 tgtagaagaa ggacaacaaa tgcgtttagc tggtcaagga gaagctggag aaaacggcgg
    18661 accatttggt gatttgtatg tggtttttcg tgtagaagaa agtgatatct ttgatcgtga
    18721 aggttctgaa atttactatg aattgccttt aagttttgta caagcagctt tgggggatga
    18781 agtccaagta ccaactgtcc atggagatgt gaaattgaag atccctgctg gaacacagac
    18841 aggtacgaac ttccgtttac gtggaaaagg cgctccgaag ttacgtggtg gtgcaacagg
    18901 cgaccaacat gtcaaagtaa aactgatcac accgaaaaat ctaaatgaac aacaaagaga
    18961 agcattacgt gcattcgctg aagctggcgg catcaaagtt gaagaacagc aagaagacgg
    19021 tttcttcgat aagtttaaag atgcttttgg cgggaaaaag cggaagtagt ttttctagta
    19081 aaaatataaa gtagacttgc ccaaattttc tatttctagt aaaaacgcta agtcattaat
    19141 aattaaaaac cagacgaaca atcatacgtt cgcctggttt ttttgtagtt gtcatgtttg
    19201 taccaacaaa aaagccagct ttagaataag ataatcatca tagtttctga aggtggtatc
    19261 gactatgtac ttgatatcgt caatgaaccg atggtacttt cttatgcgat ggaacaggta
    19321 gagcgattgc ttgtttgtgc aggagaacaa gcgatgcggc tggtacagtt aagtgaaaaa
    19381 acaagaaact aaaaatgccc ggtcgtcatg aagcgaccgg gcatttttgg tttttattaa
    19441 atatttaata ctttattcaa gaaatctttt gtccgttcat tttgcggatt ttcaaagatt
    19501 tgttgaggcg ttccgtcttc taaaaagttt ccgtcgtcaa tgaacatgac acgattggcc
    19561 acttctttag cgaagcccat ttcgtgcgta acgataacca tcgtcatacc ttgttctgct
    19621 aactttttca tgacgttcaa gacatcaccg accatttcgg ggtcgagtgc agatgtaggc
    19681 tcatcaaaaa gcatgatgtc agggttcatc gctagagcac gagcaatagc cacacgctgc
    19741 ttttgtccac cggaaagcat ttcaggatac atatcttttt tatcttctag accaactgtc
    19801 tgcagcaatt tgattgcttt tttctctgct tcttctctag attctttctt caggtccatc
    19861 ggtgctaaaa tgatgttttc caaaatagaa agatgaggga ataggttaaa gtgctggaat
    19921 accattccga tatgttgtct caccaagttg atatctgtgc ttttatccat caagtgggcg
    19981 ccgtcaataa taatttcacc gtcagaaggt tcttctaaac gattcaagca acgcaaaaat
    20041 gtactttttc ctgaacctga agggccaatg atacagacga catctccttc gttgatggac
    20101 acattgatgt cgtttaatac tgtgttatcg ccgtattttt tgactaaatg ctcaactaag
    20161 attttttctg ccatcttact tcaccttctt ttctaaaact tttgctaatt ttgtcaatgc
    20221 tgtgatcaaa atcaagtaca tgatcgcaat gatcaagtaa acatacgtac tttgtgttgt
    20281 acgtgcaaca atgatcttac cagtttgcag caattcgact acaccgattg cggaaatgat
    20341 cgttgtgtct tttaatgaga taacgaactg attgacgaat gatgggatca tgatgcgtat
    20401 cgcttgcggc aaaatgattt tttgcattgt ccgattataa gaaagaccta aacttctgga
    20461 agcttccatt tgtccgacag gaacggcgtt gatcccgcct cgaacaatct ctgcgatata
    20521 agcacttgcg ttaagagtca aggtgataac accagcaagg aagtcaggaa tcgtaaatcc
    20581 aacgatacca ggcaacccaa agaaaataaa gaacgcaaga accatcattg gaattcctcg
    20641 gatgatatcg acataaatac tagcaattgt gcgaagagcg cgtattggtg ctacactgaa
    20701 taatccaaaa atcacaccga ttactatagc caaagcaaag gagatcaaag ttaggagaat
    20761 tgttctccaa agtcctttca acaatgcttg atagttgttt tggataagtc caagaaatgt
    20821 tgattcatca gcagtggaag actcactgga agtttctgaa ccatccgcta catattcttt
    20881 aataatctta tcgtattcgc ctgttctttt taattctttc aatccttcat tgaacatatt
    20941 aagaagctca ggattttgtc cttttttaac ggcaaatgca tattttccgc cagattcttt
    21001 tttgatcggt gtttccaatt cttgtccttg tttgatcgca taaccgatta ccggataatc
    21061 atccatcatt gcatcgattg cgccaacttt taacccgtcg tacaattgat cagcagcatc
    21121 aaaatatttg atagaatagc catattcaga ttcatggtcg cttaaaaatt cagcactttc
    21181 ggtaccaact tttgctccga ctgtttttcc tttcaaatct tcataagact tgatcgaagt
    21241 atctcctttt ttgacagcta attggatccc gctatcaaag taagaatcag aaaaatcata
    21301 gcttttcttt cgttcatctg ttacagtcat tccggcaatc attccgtctg cttgtcctga
    21361 ttccaaagct tgcaaagcag cagagaaacc acggtagtcc attcggatgt taaatccttg
    21421 cagtttggcg atgttttcca tcaattttac atcgatgcct tcgtactcgc cctttgcgtt
    21481 ttgaaattca aatggagcaa aagcagagtc actggcaatt acgtatacgt cttttttggg
    21541 ctctattttt tgcattgtct cgcttgtgct tgactctgcc gttgtagtgt tgtcagcttg
    21601 tgcaacatgt ccaaacgcaa gtgtcacaag tcctgtcatt aagaaaacaa atgagtgaaa
    21661 taaatgcttt cttttcataa tcaacctcct aaatttccaa tattttatta ttataggcga
    21721 aaagtgctga atcagacaag actgtaagct cgaaaaaatg aaaaaaatgc gagtttctaa
    21781 cattttatgt tataaaacgt aacatgcata ataagataaa aaagtggcga aagtatgttc
    21841 ggtgtggtat aattttttag taaggaatgc ctttgctttc cttttctttt tattggtcgt
    21901 aaaatgaaaa ggaatgaatg aaaaggagga cgaagaatga ttgagagaga tacctcccga
    21961 cactttgagc ttgtctcgaa ataccaaccg gctggtgatc agcctgaagc gattaaccaa
    22021 ttagtggatg gcgtagtcgg cgggaaaaaa gcacaaatct tattaggtgc gactggaaca
    22081 gggaaaacat atacgatttc caatcttatt gagaaagtga ataaaccaac actaattatt
    22141 gcccataaca aaacattggc tggtcagctg tacggggaat tcaaggaatt tttccctaac
    22201 aatgctgttg aatattttgt ttcttattat gattattatc agcctgaagc ttatgtgcct
    22261 tcaagcgata cgtatatcga aaaagattca agtgtaaatg atgagatcga caaattacga
    22321 cattcggcta ctagttcatt attagaaaga aatgatgtga tcgtcattgc atccgtttca
    22381 tgtatttttg gattaggttc gccttttgag taccaaaaac aagtagtttc gattcgacag
    22441 ggagcagaac tagatcgcaa tcagttgatt cgtgacttag tcagtatcca attcgaaaga
    22501 aatgatatag attttcagcg cggtcgtttt cgagtgcgcg gtgatgtcgt ggagattttt
    22561 ccggcttcaa gagatgaacg tgccttacgt gtggagtttt ttggagatga ggtcgaacgt
    22621 atccgagaag tcaatgcact gacaggtgaa gttttaggag aaacggaaca cgtggcaatc
    22681 ttcccggcga ctcactttgt gacgaatgat gaacatatgg agcatgccgt agcgaatatc
    22741 aaagcggaat tagaacagcg tttaactgtc ttgcgaaatg aaaacaaact tctggaagct
    22801 caacgtttag aacagcgaac caattacgat attgaaatga tgttagaaat gggctacacg
    22861 tcgggaattg aaaattattc tcggcatatg gacggacgta aagaaggtga accgccttat
    22921 acgctattgg atttctttcc tgaagatttt ttgatcgtgg cggacgaatc gcacgtaacc
    22981 atgcctcaag tcagaggaat gtataatggt gaccgagcga gaaaacagat gctagtagat
    23041 tacggtttcc gtttgccatc tgctttagac aatcggccac ttcggttaga agagtttgaa
    23101 aaacatgtca atcagatcat ttatgtctct gcgacgccag gaccatatga acatgaacaa
    23161 acagataccg ttatccaaca aatcatccgt ccgacagggc tgcttgaccc agtgatcgaa
    23221 gttcgtccaa tcatgggaca aatcgatgat ctagtaggcg aaataaatga acgagtcgaa
    23281 aaagaccagc gggtgtttgt aacgacattg acgaaaaaaa tggcagaaga cctcacagat
    23341 tatttcaaag aattagggat caaagtcaaa tatttgcata gtgatatcaa aacgctggaa
    23401 cgaacagaaa tcattcgaga tcttcgatta ggtgaatttg atgtgttagt tggaatcaat
    23461 ttgcttcgtg aaggattaga cgtacctgaa gtttcccttg ttgctatctt ggatgcggat
    23521 aaagaaggct ttttacgtag tgaacgttcc ttagtccaaa cgattggacg agctgctcgg
    23581 aatgaagaag gtaaagtaat catgtacgca gacaaagtaa cagattctat gcgtctagcg
    23641 atggatgaaa catcaagacg ccgtacgatt cagcagaagt acaatgaaga acatgggatc
    23701 gttccaaaaa cgatcattaa agaaatccga gatttgattt ctattacgaa agaatcggaa
    23761 gatgatacaa aagaagcggt acaagtctcg tatgaagaaa tgacgaaaga agaaaaagat
    23821 acgttattga tgaaattaga aaaagaaatg aaaaatgcgg cgaaagcatt ggattttgaa
    23881 acagccgcta atgtgcgaga tatgatttta gaattaaaag cttcaaagta ggaggacaac
    23941 atggctaacg ataaaattac gattcacggt gcacgtgccc ataacttgaa aaatatagat
    24001 gtaacgattc cacgagataa attcgtggtc gttacaggtt tgtccggttc tggaaaaagt
    24061 tccctagcat ttgatacgct gtacgctgaa gggcagcgga gatacgtaga gagcctttct
    24121 gcttatgctc gtcagttttt aggacaaatg gataagccgg acgtagatag catcgacgga
    24181 ttgagtccgg cgatttctat tgatcagaaa acaacaagta aaaatcctcg gtcgacagta
    24241 ggtactgtga cggaaatcaa tgactattta cgcttgttgt ttgcacgtgt aggacatccg
    24301 atttgtccca acgatcatat cgagattacc agtcaatcag tagatcaaat ggcagatcaa
    24361 gtaatggctt tgcctgagcg cacaaagatc caagtgttgg caccgatcgt ggtaaagaaa
    24421 aaaggacaac ataaaaaaat attcgaacga atccaaaaag agggatacgt ccgcgttcga
    24481 gtcgatggtg agatctatga tttaagtgaa gtgcctgagc tggagaaaaa caaaaaacat
    24541 gatatcgcga tcgtcatcga ccggatcatt gtgaaagaag ggattcgttc tcgtctattt
    24601 gattctttag aggcggctct gcgattggca gacggctatg ctctggttga cgtgatcggt
    24661 gaagaagaaa tgttgtttag cgaacactat gcctgtcctt attgcggatt tactgttggc
    24721 gaactagaac caagactgtt ctcattcaat gcgccttttg gagcttgtcc agactgtgat
    24781 ggtttaggtg tcaaattaga agtcgatcaa gatttagtga ttccagatag aacgaagaca
    24841 cttagtcaag gtgctatcgt tccatggaat ccaatcagct ctcagtatta tcctcaaatg
    24901 ttagcgcagg cttgtgaaag tttcggtatc gatttagata caccattcga agaattaccg
    24961 gaagagcatc aggatatcat cttgaatggg acagatgatc ttttccattt ccattatgaa
    25021 aatgattttg gtggcgttcg cgatgtagaa acgactttcg aaggggtatt gaagaatatc
    25081 gagcgtcgtt atcatgatac gaacagtgat ttcacgcgtg agcaaatgcg tctttatatg
    25141 acagaattaa cttgtcagac atgtaaaggt tatcgattga atccccaagc tctatctgtt
    25201 caaatcaatg gaacgaatat cgggcaaaca aatgagttgt ctattcaaaa agcagtagat
    25261 ttcttctcaa acttaacgtt atctgaacaa gagaaagtaa ttgccaaacc gattttaaaa
    25321 gaagtaaatg acagactttc attcttggaa aatgtgggac tagactatct aactttgagt
    25381 cgcgcttccg gaacattatc tgggggagaa gcgcagcgaa tcagattggc tacacaaatt
    25441 ggatcgaatc tttctggagt tttgtatatt ttagatgaac catcgatcgg attgcatcaa
    25501 cgcgacaacg atcgactgat tggctcatta aagaaaatgc gtgatcttgg caacaccctc
    25561 gttgtagtcg aacatgatga agatacgatg tgggcagcag attatctgat tgacgtcgga
    25621 cctggggccg gagaacaagg cggacaaatc gtggctgcag gcactcctga agaagtagct
    25681 aatgatgaaa attctttgac agggaaatat ctttcaggga aaaaatcaat ccctgtacca
    25741 aaagaacgcc gtaaaggtaa tgggaaagca atcaagatca ctggtgcttc agaaaacaat
    25801 ctgaaaaata ttagtgttga atttccatta ggagaatttg tagcagtcac aggtgtgtct
    25861 ggttctggaa agagtacatt ggtcaatagt atcttaaaga aatcgttagc gcaaaaatta
    25921 aataagaatt ctgctaagcc aggtaaattc aagacaattt ccggctacga aagtatcgaa
    25981 aagatcatcg atatcgatca aagcccaatc ggccggacgc cgagaagtaa tccagcgact
    26041 tatacaagtg tatttgatga tatccgtggg ttatttgctc aaacgaacga ggcaaaaatg
    26101 cggggttata agaaagggcg ttttagtttc aacgtaaaag gcggtcgttg tgaagcttgt
    26161 cgcggggatg gaattattaa gatcgaaatg cactttttgc ctgatgtcta tgttccttgt
    26221 gaagtatgtc atggcaaacg atataactct gaaacattag aagtgcatta caaaggaaaa
    26281 agcattgctg atattttgga aatgacagta gaagatgctg tagaattctt caagcacatt
    26341 ccaaagattc atcgcaaact gcaaacgatt gttgatgttg gcttaggtta tgtgacgatg
    26401 gggcaaccag caacgacatt gtccggtggt gaggcacaac ggatgaaact tgccagtgaa
    26461 ttgcacaaaa tctctaatgg aaagaatttc tatatactag atgaaccaac gacaggactt
    26521 catagcgatg acatcgcccg cttgttgcat gtattacaaa gattagtaga tgctggtaac
    26581 acagttttag tgattgaaca caatctagat gtaatcaaaa cagcagatta tatcattgat
    26641 ttaggaccag aaggtggaga aggtggagga acgatccttg cgactggaac accagaagaa
    26701 atcattaacg taaaagaaag ttatacaggt cactatttga aaaaaataat ggtataaaga
    26761 ggatagatag ctgagatagt taaaaaaaca taaaaaaatg aatttttgct tgttttatta
    26821 atatcagtgt tctagaatca ttacatggta gttctttcta cactgattgg tatagacaaa
    26881 aacttcctat aagttcaatc cgttcaaaaa agaattgagc aatcggagga gtttcctttg
    26941 attgcttttt tctttatggt atgatattcc ttgtgtagtt ggaaaagaaa aacaagagat
    27001 tatgagtaag tgagagataa tgatgaacag tattgaaaat gaagaattta tccctatatt
    27061 attaggaagc gacatgaacg tttatggtat ggcacgttct tttaatgaag catacggcaa
    27121 aatctgccaa gcatatgctt cggatcagtt agcccctacc cgctacagta aaattgtcaa
    27181 tgtagaagtt attccaggat ttgataaaga ccctgtgttt atcgagacga tgcttcgttt
    27241 ggcaaaagaa agatattctg ataagagtaa aaaatatctt ttgattgctt gtggtgatgg
    27301 atatgcagaa ttgatctccc aacataaaca agagctgtca gaatatttta tctgtccata
    27361 tattgattat tctttatttg aaagactgat caataaagta agcttttatg aagtttgtga
    27421 agaatatgat cttccgtatc ctaaaacatt gatcgtcaga gaagaaatgt tagtaaatgg
    27481 acatttggaa caggaattgc cttttgaatt cccagttgct ttgaaacctg caaacagtgt
    27541 ggagtatctt tctgtccaat ttgaaggaag aaaaaaagct tttattttag agactagaga
    27601 agagttcgat ttaattttag gccggatata cgaagccggt tataaaagtg aaatgattgt
    27661 acaagatttt atcccgggtg atgatagtaa tatgcgtgtg ctaaatgcgt atgtagatga
    27721 agatcatcaa gttcggatga tgtgtcttgg tcatccactg ttagaagatc ctacaccagc
    27781 atcgattggt aattacgttg tgatcatgcc agattataac gaaaaaatct atcaaaccat
    27841 taaggcattt ttggaaaaaa tcgaatatac aggctttgcc aatttcgata tgaagtatga
    27901 tcctagagat ggagaatata aattatttga aatcaatttg agacaaggtc gtagcagttt
    27961 ctttgtgaca ttgaacggct tgaatctggc tcgtttcgtc acagaagatc gagtgtttaa
    28021 caaacccttt gttgaaacga cctatggaac gaatcaatca gataaagcaa gattatggat
    28081 gggtgtgccc aaaaaaatct ttttagaata tgctcgggaa aatgaagaca aaaaactagc
    28141 tgagcaaatg atcaaagaaa atcgttacgg tactactgtg ttttatgaaa aagaccggtc
    28201 aatcaagcgc tggttgttga tgaaatatat gttccataac tatattccaa gattcaaaaa
    28261 gtattttcat gtgaaagaag gctagttcat ggagaatttt ttcagtattt taggcggaat
    28321 gggcacgatg gcgacagaaa gttttgttcg cttgatcaac caccgaacga aagctacaaa
    28381 agatcaagaa tatttgaatt atgtcttatt caatcatgca acagttcctg atcgtacagc
    28441 ttatatttta gatcgatcag aagaaaatcc aatgccgttt ctgctggatg atattgagaa
    28501 acaaaattta ttgcagccga acttcattgt gttgacttgt aacaccgcac actacttttt
    28561 tgaagaatta caagcggcaa cggatattcc tatccttcat atgccaagag aagcagcaaa
    28621 tgaacttgtc cgtcagcata cgacagggag agtagcgatt ctaggaacag aaggaagtat
    28681 gaaagctgga atctatgaac gcgaagtcaa aaatcttgga ttcgaaacag tgattcctga
    28741 tactgttctt caagaaaaaa tcaattatct gatctatcat gagatcaagg aatcggatta
    28801 tttgaatcaa gagctatatt acgaaatctt agaagaagcg gtagagcgcc taaactgtga
    28861 aaaagttatc ttaggttgca cagaattgtc attgatgcat gaatttgctg aagataacca
    28921 ttatccagtg atcgatgccc aatctatttt agcagatcgg acgattgaac gagcgctagc
    28981 tgaaagaagc aaggcattgg atacagcatc ggaaaagtaa aaaaattgga tttggggcaa
    29041 atatcctaaa agatatttgg tccaaatctt tttgtaaaag aaaaagaaaa ctggtaatga
    29101 tacgcccgga atgatataat tttactatgc acaatgtgag aggagttcgt aattatgaca
    29161 gataatttgc aattagtcat cataacaggt atgagcggtg cagggaaaac agtagctatc
    29221 caaagttttg aagatatggg ctatttttgt atcgataata tgccgcctcg attgattcca
    29281 aaattctggg aattaatcaa agaatcaggc aaagtcacaa aaatggcttt aggagtggat
    29341 ttacgttctc gttccttctt tgaagaaatc caaaacatgc tgatcgaaat cgaaaatacg
    29401 aacttcatcg acacttctat cttattttta gatgcttcag acgaagaact agtggctcgc
    29461 tacaaagaaa caagacgagc acatccaatg gcgatggacg gtttgatcac tgaagggata
    29521 cgtaaagaac gggcgatact agacgacttg aaagcccaag cttccgtgat cattgatacg
    29581 acgacattga caccacgaca attacgtgaa aaaatcaatg agtcattcaa atcaaaggga
    29641 gaccaaggat ttcggatcga atttgtatca tttggtttta aatacgggtt gccgatcgat
    29701 tcggatattg tgatggatgt gcgttttttg cccaaccctc attatatccc ggaactaaga
    29761 ccattgaccg gaaaagatca gccagtctat gattatgtga tgtcctttga tgaaacgcaa
    29821 tcattctacc ataaattttt ggatcttctg gaaactgtat tgcctggata cattaaagaa
    29881 ggaaaaagca gcttgacagt tgcaatcggc tgtacaggcg gtcaacaccg ttctgtggca
    29941 ttgactgaac gtttggcaaa tgctttaggg aaaaactata aagtcaatgt gacacatcgg
    30001 gacaaggata aaaggaaaga gacggtgaac cggtcgtgaa aatgaaaacc taccggataa
    30061 gaaaaccaaa gatcgtagtg gtcggcggtg gcactggtct gccagttatc ctgaaaagtt
    30121 tgcgaaatca aggagcagat atcacagcag tagtgactgt tgcagatgat gggggaagca
    30181 gcggtgcaat cagagaatcg atcgctatgg ctcctcctgg tgacttgaga aatgtcttag
    30241 tggcgttatc ggatatgcca caattctatg aagatatttt ccaataccgt ttcaaaaagg
    30301 aagatcagtt tttagccaat catacgatcg gtaatctgat cattgccgca gtttccgaga
    30361 tgcggggaag cacttatgag gcgattcaat tattagcaaa aatgatgcat gtagatggac
    30421 atgtttatcc ttcttcagaa actcctttga ccttgcatgc tgtctttaaa gacggaacga
    30481 ctgcagtagg agaatctaaa atcgcgatcg accgtaaaac cattgaccgt gtgtatgtct
    30541 gcaacacaaa tgataaaagt gaagccaaag cggctcgaaa agtcgtttca gcaattatgg
    30601 acgccgacat ggtcgttttg ggacccggaa gtttattcac cagtatcctt ccaaatttag
    30661 tgataccaga aatcggcgaa gcaatgctca agacaactgc tgaaaccgtc tatatctgta
    30721 atatcatgac ccaaaaaggg gaaacggaac atttcacaga tgccgatcat atacgtgttt
    30781 tacacaagca tttgaatcag ccttttatcg atacagtatt ggtcaatacg gaaaaagtgc
    30841 ctgaagatta tatggaccca gagatctatg atgagtatct agtacaagtc gcacacgact
    30901 tcaacggatt gagaaatgaa ggatgtcgag tgatttccac cgactttttg gaattgaaag
    30961 atggtggtgt cttccacgat ggagacaaag tagtagaaga attgttccgt ttagttttcg
    31021 gttcaaaata ttaagcaatt tattgttgga gaaaggagga gaataggatg tcatttgctg
    31081 cagatgtgaa gaaagaacta actggtttgg aagttcatcg agaacatgcg aaagcggaat
    31141 tagctgcttt gctgcgaatg aacggctctt taggtattgt gaaccagcag tttattttga
    31201 acgtccaaac agaaaatgct gcaattgcca gacgtatcta ttctcttttg aaagatcatt
    31261 atcaggtacg aagtgagcta ctcgttcgta aaaaaatgaa attaaaaaag aacaatgtct
    31321 atatcgtccg gttgaagcaa ggcacaaaag atgtgttgat ggatcttgat atcatggatg
    31381 gtatgatgtt caatggacac gtttcggacg agattatggg aaattcgcaa aaaatgcgtt
    31441 cttacttacg tggtgctttt atggcctcag gttcagtcaa taatccggaa acgagccgtt
    31501 atcatttgga aatattttct atttatgaac agcataacca agacatttgc gatatgttaa
    31561 attattacgg tctacatgct cgaacgcttg aacggcgcaa tggctatatt tcttatttaa
    31621 aaggagcaga aaaaattgct gatttcttaa cattgattgg tgcaaccaac tcaatgctcc
    31681 gatttgaaga tgttcgaatc gtacgggata tgagaaattc ggtcaatcgt ctagtcaatt
    31741 gtgagacagc aaatctgaac aagacgattg atgcggcttc taaacaaatc gaaaacatcg
    31801 aattgatcga agcaaaggtc ggtctacatg cccttcctga aaaacttcag gaaatcgcag
    31861 aacttcggct tcaacatcca gaggtcagct tgaaagaatt aggggaaatg attccctcag
    31921 gtgcgatatc aaaatcggga attaatcacc gaattcgcaa aataaatgaa tttgcggaaa
    31981 aactgcgtga agaagcagtt taatccaaag aaaaaactga tacatgaagc aggagatgcg
    32041 aatgtatcag ttttttttaa aagttatact tgcttttctt ccgtcaaact ataattccat
    32101 ctttactgga gtaacataat ttggaaaggc ttctattgtt tttaatacaa tcgtataaaa
    32161 agcggggaga caactgaatg aagattttag ttttgtacat aaatttttgc ttaatataga
    32221 agtcctgata tagataagat caagtattta tctataactg gcctgattaa ttaaaaaact
    32281 ggaaggaact gctcgatcat ccttgagaac tgctcttctt acttctggtc aagattctcg
    32341 tggttggtgg ataaggatgg ttgataagtt cattggatta gctagtcgaa aacaaccagg
    32401 tgtaattaat gtagaagttt taacaagggg atggagtgat ttattcagtg ataatcataa
    32461 gttcataaat agtaattgag atgtatggtt ggaatacgtt atacctgaac ttgaactacc
    32521 aagatttggt ttagaatttg agaatcgatg tttataaagg gagggcaaaa atgcgttttt
    32581 ttacaacttt tactatcatt atgattgcag tactttttat ttttctagat atagcaaagc
    32641 gaaatacagc atttttatta tatcgggttt tacttcgagc aggtctcata acatttatat
    32701 cgatcgttgg attttttcta ttcacagtta ttgtatttat ttggagaact ccagcaccac
    32761 cattgccaga gattacatat ggagaatttc cttttagact tgagtatgaa ttgaatgaag
    32821 aattacatgt gatagaagac acgttaatag ttgaatttga tggatttgga atgaacgaag
    32881 gcatagggag atatcgtaga tggacaagtc gtttagccag cggagaagat ttagtgttgc
    32941 tgttagaagt tagtgataat aagcaaatat tttattttcc aggacctgct aattattaca
    33001 tgggagatag gttgaatggt tataatcata cttttcctag tgcttctttc attgaaaggg
    33061 aaagaggcat tactagaagg gatattttac atgacaaaga attattagaa caatttggtc
    33121 cgcttgatca aaatacaata aatgaagaag aattgttaaa tcaatataat ataaggctag
    33181 tgaattggga aatatctgaa ccgattgtta acaattttgg tgattagaaa acaattacga
    33241 taaatagaaa caagccaaat catcagcaag taaaagctga tgatttggcc tttcttatac
    33301 gagtagtagg aattcatagc gaatattatt acgatcagta gatattttga tattaaagga
    33361 gtattacgaa ctagtatgtg agaatgtaga ggtgatttgc gaataatgag aaatttatca
    33421 aatagaaaca aaatattaat cattattgtt gttatagctg tgtttcatct gggaacgaat
    33481 gcggttttaa gtagaataat attgggtcct aagccaccaa gacctgcgat tacccgaggg
    33541 gagtttgatt ttagattaga atatgaagta gatggggaaa ggattgtgat agaagataca
    33601 attgttgcat tatttgatgg attttctgca gatgcaggta gcatggcatg gtatcgcacg
    33661 tggcgtttgc atttggcgag tgacaagagg gcacaacaat tattgttaca tgagttagat
    33721 gatggtcgaa aaatatatta tcattttgga ggagctggat tttatatggg gaagttagta
    33781 gcagtgtaac tgtaacaagg aaagatttgc caggtcttgg cattcgaaac tccatatttt
    33841 atatcgagcg tgggagagat acatctattg ggaacaggaa agggttaact cttgaagagt
    33901 tatataatga ctttggaatc cgtcttgtta gttgggaaca caatccacca atagaaaatc
    33961 gctttgaata atcgaaatct attaatccat ttcatcagcc agtgaatgtt gtaatggaac
    34021 aatttggttt agtgttcaaa accattgaaa tactaggctg tgatcagcaa aaatgtatct
    34081 gaataaaaaa gagagcaact attaaatgag aggagttagg agttgttagc tttatacgaa
    34141 tcaaagtggc aaatacagat gcgtatgcat ttgtgtttgc catcttgcgt ttggtagacg
    34201 agataggtat aagctaaaga aacaacttgt ttaaaataaa cgacctagct caacaaacga
    34261 ccatctaatc aactaacagc atacataaaa gcaactattt ttgaagtaga attgaaatcg
    34321 ggataactta aaaagtgatt gggatactag tgtgaatcac cgtacataaa tattactcgt
    34381 taagggaatt tgagaaattt tgtatgaaaa tttaagaatt agttagtatg cgcaagtcaa
    34441 aaatatgaat aaattatttg gtggagataa tttgctcagc ttgtcataag aaatgagata
    34501 atctggcaga tgcaagcttt tggacaccat ttattcggaa taaggaggct gggacagaag
//
LOCUS       DBNICI010000010        33832 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.010, whole genome shotgun sequence.
ACCESSION   DBNICI010000010 DBNICI010000000
VERSION     DBNICI010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 33832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.010"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            147..1016
                     /locus_tag="AC1ZCC_000411"
     CDS             147..1016
                     /locus_tag="AC1ZCC_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIQ1628694.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMINFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="AC1ZCC_000412"
     CDS             1225..2250
                     /locus_tag="AC1ZCC_000412"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIQ1628695.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="AC1ZCC_000413"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="AC1ZCC_000413"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HIQ1628696.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3333..5036
                     /locus_tag="AC1ZCC_000414"
     CDS             3333..5036
                     /locus_tag="AC1ZCC_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIQ1628697.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="AC1ZCC_000415"
     CDS             5038..7071
                     /locus_tag="AC1ZCC_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HIQ1628698.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="AC1ZCC_000416"
     CDS             7082..7522
                     /locus_tag="AC1ZCC_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIQ1628699.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="AC1ZCC_000417"
     CDS             complement(7722..8849)
                     /locus_tag="AC1ZCC_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1628700.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="AC1ZCC_000418"
     CDS             complement(8852..9529)
                     /locus_tag="AC1ZCC_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIQ1628701.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="AC1ZCC_000419"
     CDS             9793..11559
                     /locus_tag="AC1ZCC_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HIQ1628702.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="AC1ZCC_000420"
     CDS             11574..12011
                     /locus_tag="AC1ZCC_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIQ1628703.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="AC1ZCC_000421"
     CDS             12024..13175
                     /locus_tag="AC1ZCC_000421"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HIQ1628704.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13237..13935)
                     /locus_tag="AC1ZCC_000422"
     CDS             complement(13237..13935)
                     /locus_tag="AC1ZCC_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HIQ1628705.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13928..15280)
                     /locus_tag="AC1ZCC_000423"
     CDS             complement(13928..15280)
                     /locus_tag="AC1ZCC_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HIQ1628706.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15503..16903)
                     /locus_tag="AC1ZCC_000424"
     CDS             complement(15503..16903)
                     /locus_tag="AC1ZCC_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIQ1628707.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17064..17801
                     /locus_tag="AC1ZCC_000425"
     CDS             17064..17801
                     /locus_tag="AC1ZCC_000425"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HIQ1628708.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17926..18405
                     /locus_tag="AC1ZCC_000426"
     CDS             17926..18405
                     /locus_tag="AC1ZCC_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HIQ1628709.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18402..19169
                     /locus_tag="AC1ZCC_000427"
     CDS             18402..19169
                     /locus_tag="AC1ZCC_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HIQ1628710.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19399..19968
                     /gene="lepB"
                     /locus_tag="AC1ZCC_000428"
     CDS             19399..19968
                     /gene="lepB"
                     /locus_tag="AC1ZCC_000428"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIQ1628711.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20072..20674)
                     /locus_tag="AC1ZCC_000429"
     CDS             complement(20072..20674)
                     /locus_tag="AC1ZCC_000429"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIQ1628712.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20780..21010
                     /locus_tag="AC1ZCC_000430"
     CDS             20780..21010
                     /locus_tag="AC1ZCC_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628713.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            21001..21249
                     /locus_tag="AC1ZCC_000431"
     CDS             21001..21249
                     /locus_tag="AC1ZCC_000431"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1628714.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            21355..21939
                     /locus_tag="AC1ZCC_000432"
     CDS             21355..21939
                     /locus_tag="AC1ZCC_000432"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HIQ1628715.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            21942..23015
                     /gene="prfA"
                     /locus_tag="AC1ZCC_000433"
     CDS             21942..23015
                     /gene="prfA"
                     /locus_tag="AC1ZCC_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIQ1628716.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23008..23847
                     /gene="prmC"
                     /locus_tag="AC1ZCC_000434"
     CDS             23008..23847
                     /gene="prmC"
                     /locus_tag="AC1ZCC_000434"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIQ1628717.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23840..24019
                     /locus_tag="AC1ZCC_000435"
     CDS             23840..24019
                     /locus_tag="AC1ZCC_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628718.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24041..25066
                     /locus_tag="AC1ZCC_000436"
     CDS             24041..25066
                     /locus_tag="AC1ZCC_000436"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIQ1628719.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25257..26501
                     /gene="glyA"
                     /locus_tag="AC1ZCC_000437"
     CDS             25257..26501
                     /gene="glyA"
                     /locus_tag="AC1ZCC_000437"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIQ1628720.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26654..27601
                     /locus_tag="AC1ZCC_000438"
     CDS             26654..27601
                     /locus_tag="AC1ZCC_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HIQ1628721.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27735..28364
                     /gene="upp"
                     /locus_tag="AC1ZCC_000439"
     CDS             27735..28364
                     /gene="upp"
                     /locus_tag="AC1ZCC_000439"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HIQ1628722.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28503..28745)
                     /locus_tag="AC1ZCC_000440"
     CDS             complement(28503..28745)
                     /locus_tag="AC1ZCC_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628723.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28866..28938
                     /locus_tag="AC1ZCC_000441"
     tRNA            28866..28938
                     /locus_tag="AC1ZCC_000441"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28899..28901,aa:Thr,seq:cgt)
     gene            complement(29057..29695)
                     /locus_tag="AC1ZCC_000442"
     CDS             complement(29057..29695)
                     /locus_tag="AC1ZCC_000442"
                     /EC_number="3.4.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HIQ1628724.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29818..30450)
                     /locus_tag="AC1ZCC_000443"
     CDS             complement(29818..30450)
                     /locus_tag="AC1ZCC_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HIQ1628725.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            30801..31100
                     /locus_tag="AC1ZCC_000444"
     CDS             30801..31100
                     /locus_tag="AC1ZCC_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HIQ1628726.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            31595..33760
                     /gene="acm"
                     /locus_tag="AC1ZCC_000445"
     CDS             31595..33760
                     /gene="acm"
                     /locus_tag="AC1ZCC_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HIQ1628727.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
BASE COUNT        11702 a         5819 c         6774 g         9537 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattaatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gtctgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt ctggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccatgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaacgtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg acgaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcggaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggcag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatcca gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaagag
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttcgct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata
    13261 gggcagatcc aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc
    13321 caggttctca tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta
    13381 aatgacacct tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa
    13441 aggtcgttca ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt
    13501 ttcattgaat tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact
    13561 taacgtgtag tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta
    13621 gtagtgacct agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat
    13681 atagttgatc aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa
    13741 gtcttgacca ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat
    13801 actaacgatc tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa
    13861 aaggtttccg ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag
    13921 ttcataatta gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac
    13981 gaagctgcaa tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt
    14041 tgattttttc gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc
    14101 cggcaacttt tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg
    14161 ggatatccat atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc
    14221 cgtctgcgta attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa
    14281 tcatatcgat tacttgattg atacctacag gatttttgac aagtactaat gtacattttt
    14341 tcccattgat attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt
    14401 gtttgatttt atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat
    14461 tgtagacatt atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat
    14521 ctgctgacac attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt
    14581 tgaagtcaca atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat
    14641 gcaagatgtg atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt
    14701 cacgatcttc ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat
    14761 tgaagattgg tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt
    14821 ctacaatcat tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa
    14881 tatttgtaaa taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt
    14941 catctatttc taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag
    15001 tcgatacaat tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt
    15061 gctggcgcaa aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga
    15121 caacgacgac ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg
    15181 ctagtttacc tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg
    15241 tttttccaac tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga
    15301 tttgctgacg aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt
    15361 ttcttattct taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga
    15421 tttaacaaaa aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt
    15481 ccctcttacc tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc
    15541 ctagatctcg tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc
    15601 caataaacat cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc
    15661 ctaaagataa accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg
    15721 tttgttcttc tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca
    15781 taaagacaac gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga
    15841 tgttatcttc acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg
    15901 ttcgaatccc gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa
    15961 tgatcagtgt tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag
    16021 tcgtaatcga ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata
    16081 aatggtcctt ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag
    16141 ctaacacagc taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt
    16201 tgtataatag ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa
    16261 tcaaaaacat gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc
    16321 cagtgacgtc aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa
    16381 cggcatcacg ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc
    16441 caaaaagtaa ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac
    16501 cagacaaacg cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc
    16561 gtcgtttaga aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt
    16621 gaaataatat ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag
    16681 tcgatagtcc agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa
    16741 catggctatc tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca
    16801 tcaatatata ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct
    16861 gtcctcgccg ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac
    16921 aatagtcgtt tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca
    16981 gataacaata cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa
    17041 aaagaaggag gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa
    17101 atcaaaaaat aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag
    17161 aagtaaagcc taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg
    17221 aattcatgtc tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta
    17281 aaagtgagta tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga
    17341 aaactgacca tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa
    17401 tggttttaac aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct
    17461 tcaatatgcg attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg
    17521 gaagtgccct gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg
    17581 atatccaaaa gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct
    17641 atgaacgctc tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt
    17701 gcgtgttcgc tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag
    17761 cttacttaaa aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac
    17821 gtaagaaaaa aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag
    17881 taaagtaagg aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca
    17941 cctattttga agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt
    18001 attgtgaata gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca
    18061 acaccaacta cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc
    18121 actacgacaa gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca
    18181 actgacagct caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc
    18241 aatccaagta caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact
    18301 gtagatgata acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca
    18361 ggaaataccc aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc
    18421 ttatcaacgg ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc
    18481 gtggatatac gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac
    18541 tttggcaaaa ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg
    18601 tttattttac tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg
    18661 tcactcagct ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat
    18721 cgatagataa tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg
    18781 acgaagatta tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga
    18841 tcaaaacaaa tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc
    18901 cttattacta tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct
    18961 actcggacat ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac
    19021 ctgtcggatt gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc
    19081 cttatcgtct gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata
    19141 gtcgtagcca aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg
    19201 ataaaaacgt ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt
    19261 atataacgag aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt
    19321 gtagaataaa gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat
    19381 acaagtagga ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta
    19441 ttagcggcta ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat
    19501 tcgatggacc ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat
    19561 gaacgaatgg atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa
    19621 cggatcatcg gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat
    19681 ggcaaaaagt atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag
    19741 ttacaaggag aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc
    19801 tttacgagtg actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac
    19861 cgcttgattt caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga
    19921 aaagttgttt tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa
    19981 aagaagctgt gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct
    20041 tggagttgct cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa
    20101 gcgaagcgtt tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag
    20161 agacaaagcc cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga
    20221 ctttcggatg actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag
    20281 gagctaaaat ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta
    20341 caagtgatag aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt
    20401 ggatcgttcg gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca
    20461 tagtttctga agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa
    20521 caagcccagt tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat
    20581 aagtagttag ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat
    20641 ggttagcgat ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg
    20701 aaattttaac ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa
    20761 gggagaatct agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa
    20821 gtgtgtccga gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga
    20881 aaaatatatc gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata
    20941 tttaggcttt atcactcttt cacagacaag tgaagatact gccgaaattt attctattgt
    21001 atgggaataa aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca
    21061 gaaggttacg cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac
    21121 tactctatat atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa
    21181 gtttttccaa atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa
    21241 caaaagtgag caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa
    21301 atgctgaaaa tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca
    21361 caactatttt ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc
    21421 gcacataatt atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca
    21481 agagatggtt taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata
    21541 tttgaagaaa cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg
    21601 ctgatcgatg aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg
    21661 gacgaattgg atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta
    21721 tttgaaggat caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc
    21781 tgttggttct gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt
    21841 tatgaaggcg atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa
    21901 cattactttc acccatcaat gattacagaa ggagaataag catgtacgat caattacaag
    21961 caattgaaga ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg
    22021 atacaaagcg ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag
    22081 tttatcgtcg ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg
    22141 aaaaactgga tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag
    22201 aaaaagaagt cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg
    22261 ataaaaatat catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg
    22321 ctggcgatct tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag
    22381 tgatggaagc taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg
    22441 gagacaacgt atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc
    22501 cttcaactga atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag
    22561 aagctgaaga agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg
    22621 cttcaggtgc tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact
    22681 taccaacagg gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa
    22741 aagcaatgaa aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg
    22801 agtatgatgc caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga
    22861 cgtataattt cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat
    22921 tagatcagat tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc
    22981 aaacatcaaa actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca
    23041 cgggcttctt cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt
    23101 ttggaacgaa aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca
    23161 gaagaagaac agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct
    23221 cagtatttgc taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca
    23281 ctgatccctc gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt
    23341 gtaccgttag aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa
    23401 gcagcaagaa aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta
    23461 gcgaaagaaa atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta
    23521 ggaccagtaa tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg
    23581 aatgaatggg aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt
    23641 gctgagaacg atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta
    23701 aaatcaaatg gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa
    23761 atatttcagc aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat
    23821 gaacgaatga tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt
    23881 tattataaaa aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt
    23941 ttatccacta ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag
    24001 acaaaataag aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga
    24061 tgaaacagaa ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc
    24121 taccgaaaca gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt
    24181 gttttctgta aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga
    24241 acaagtaaaa gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt
    24301 ctggcctgga ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt
    24361 gacgggagga ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt
    24421 aatcgaactt tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag
    24481 tccaacgact gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga
    24541 tgatggagct acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc
    24601 catgcctatg attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga
    24661 atctcctgtt gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg
    24721 gatgaaatac aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg
    24781 gtcaactgca gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc
    24841 cgaaattgcg gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc
    24901 agtagaagcg gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact
    24961 tggattagat ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat
    25021 gaatcgcttg aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg
    25081 tcgcattctt ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa
    25141 ctaagcgtaa ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg
    25201 aaaaataaaa ttgtaggaaa ataaaattgt aggaaaataa aaaggagctg ttgtttgtgg
    25261 tagattacaa aacgtttgac cctgatcttt gggctgcgat cgccaaagaa gaagaaagac
    25321 aagaacataa tttggagctg attgcctctg aaaactttgt ctccgaagca gtgatggctg
    25381 cacaaggaag tatcttgaca aataaatacg cagaaggtta tccagggcat cgctattatg
    25441 gtgggtgtga atttgtcgat atcgttgaaa atttagcgat tgaccgtgca aaagaattat
    25501 tcggagcgaa atttgcgaac gtccagcctc actccggctc tcaagccaac acggctgctt
    25561 atctcgcttt ggtagaacca ggggacacga tcttaggaat ggatctttcc gcaggcggac
    25621 atctaacaca tggatcacca gtcaacttca gtggcaaaac ctatcacttt gttgcttacg
    25681 gtgttgatcc gacaacagaa gttattgatt ataatgtggt acgtatttta gctagaaaac
    25741 atcaaccaaa attgatcgta gcaggtgcca gtgcttacgg acgaacgatc gactttgcga
    25801 aattcagaga aatcgcagat gaagtaggcg caaaactgat ggtagatatg gcacatatcg
    25861 caggattagt agcagcagga cttcatccta atccagttcc ttatgcagat atcacaacga
    25921 ctaccactca taaaacattg cgtggccctc gaggcggcat gattttgaca aacgatgaag
    25981 cattagcaaa aaaaataaat agtgcggtat ttcctggtat tcaaggcggc cctctagagc
    26041 atgtgattgc gggtaaagca gtagcattca aagaagcatt agatcctgca tttaaagaat
    26101 atagtgaaca aatcatcgcc aatgcaaaag caatggtcaa agttttcaat caagcaattg
    26161 gaaccagagt catttcaggg gcgacagata atcatctgat gttgatcgat gttcgcgagt
    26221 taggtataaa tggaaaagaa gcagagagca ttttggacag tgtgaatatc actgtaaata
    26281 aaaactcgat tccttttgaa acactaagtc cattcaaaac aagcggtatt cgaatcggca
    26341 cgccagcaat cactactcgc gggttcaaag aagaagatgc tgtaaaagtg gctgaactag
    26401 ttgtcaaagc attacaagca aaagatgata acgctcaact agacgaagtc aaaacaggtg
    26461 tacgcgaatt aactgaaaaa tttccgttgc ataaaaaata aaaatttaaa ttccattagt
    26521 aaacagaaaa gttcaatgtt gggcttttct gtttattttt tgggaaaacg gattctttga
    26581 caatatcggt gatgctctct agtatatgag gtgtaatttt agtcttcaca ctaaatctca
    26641 gggaggaaaa gtcatgactg atcgtatctc gttagaaaag gaagcagtag cattcagtga
    26701 tgcaaatgcg cctcatccgc gtatctatga actagcaccg gaagaaggaa gagttttact
    26761 agaagaagta caagaatcac ctgtgaacaa atattccgtc gatattgaag acttgatggt
    26821 ggatactggc gaatggggaa aactcaatat tcgtctcgta cgaccagaag ggaatatgga
    26881 taaattacca gttatttttt atattcatgg agcaggctgg gtttttggca gtgctcaaac
    26941 ccatgataaa ttgatcagag aacttgcggt tcgtacgaat tccgtcgttg tctttccaga
    27001 atatagtcga tcaccagaag caaaatatcc aacagcaatt gaacaaaatt atgctgtgtt
    27061 gcaacagctg aataaattag ccaagtcaaa aaatctggat ctcgagaatc taatcgttgc
    27121 aggagattca gtaggtggta acatggctac agtcatgacg atcttaacaa aagaacgaaa
    27181 gggcctccca atatccaagc aattgctgta ttatccagtc acaaacgctg attttgatac
    27241 agattcctat catgaattcg gagaaaacta ttttttgaca agagaaggaa tgatgtggtt
    27301 ttgggaccaa tatacgacga atgaagaaga gcgtgctgaa atcacagctt cgcctttgag
    27361 agcaaatata aatgaactaa atgatcttcc ttcagcaatg attttaacgg gagaagcgga
    27421 tgtattaaga gatgaaggtg aggcttatgc gcgcaaactt cgtgatgcag gggttcctgt
    27481 tacacaagta cgcttccaag gaatcatcca tgattttgtc atggtaaatg ctatggatca
    27541 gacaaaggca acgcgtgcag caatggcatt atctacttct tggatcaaga aaaaagaata
    27601 agctttgctt gtccgcagga aaacttcttt tcaaaaacgg attttcttag gaaaaatggc
    27661 gagtttgact agtcaatcac ttgattttgt tttacaatta aaaagaaacg acagaacaaa
    27721 ggagagctta aaaaatgggc aaatttcaag tgatcgatca tccattgatt caacacaaac
    27781 taactattat tcgggacaaa aactgcggaa caaaggtttt tcgtgaagta gtcgatgaaa
    27841 tcgctatgtt gatggcatat gaagtatcac gggatatgcc attggaagac atcgtgatcg
    27901 aaacaccgat cactgaaaca acacaaaaaa cgttgtctgg taaaaaagta gcgattattc
    27961 caatcttgcg cgcaggtatt ggaatggttg acggtatctt ggaattgatt ccagctgcta
    28021 aagtgggaca tatcggtctt tatcgagatg aagaaacatt ggaaccacat gaatatttcg
    28081 tcaaacttcc agaagacatt gatgcacgtc agttgtttgt agtagatcca atgttggcaa
    28141 caggcggatc agcaatcatg gcaatcgatg cattgaaaga acgtggcgcg tcgaacatta
    28201 aatttgtttg tttggtagca gcacctgagg gagtaaaagc tttgcaagaa gcgcatcctg
    28261 atattgacat ctatacagct tctctagatg aaaaattgaa tgaacatggt tatatcgttc
    28321 ctggactagg ggatgcgggt gaccgtttgt ttggtacaaa ataagtaaat acgaaaaagg
    28381 gtgtgacaca acttttgtca catccttttt tggtcatttt taacgtttga tgcagaacag
    28441 ctttttcaca gctttcgttt gcatataaaa aaaccatccc aaaaatgcag agatggtcaa
    28501 attcaaatct tttttagttc tttgtagtta acgattacta cacccttact ttgcgtcatc
    28561 aagttcgcat tttctgcagt ttcatcaatc tcaacaacag cggaattttt caattgcttt
    28621 gaaatcgttc cagtttgcga cttgccgtga attgtgaaca ttacgtgagt attgatcttg
    28681 aatttttcac caatttccga tggtacgata gttgtatcaa attttccaaa agttgccatt
    28741 ttcataaaac ctgcctttca catcaattat accacgtttt atgaaaaata tcatgaaatt
    28801 aaactaattt tccatcggac tctttacaat cagccaaata gagtatataa tttcttttgg
    28861 ataaggccag cttagctcag ttggtagagc aacgcactcg taacgcgtag gtcgtaggtt
    28921 cgatccctat agctggcatt aaaagagaag acagatatat caaggatttt taatccattg
    28981 atacatctgt ctttttgttt aaaggaaata aagtatgctt tcttagcaat agagcatgta
    29041 ttatttctat aaaaatttag gagaataaaa cgactagcaa tatagctgtg aaatcaaata
    29101 tcacatgaac tgcccaactt gtaatgatcg agttgctttt aatggcagct tggttgaata
    29161 acaatcttgc caccccttgt atcaaaagga tgtgtgctac agtgtgtaaa atatttccat
    29221 tatcataagt actgtaatgg ataaatccaa agatgattgc tgacaagaaa ctagcgacag
    29281 gaatgctgaa cttactagaa aaaatagcga gaaaatacac tgagaacaat tcttctccaa
    29341 gtatcatgat aggcaaaata ataaataata acgggctgaa agcctcattt attggattgc
    29401 ctgttaaacc ccatttcaaa atttgttgca aaaataccga gactgcggca ctgattacta
    29461 cattgataaa aaaatatttg gctatatttt tgacaggagc gacaggtttc gagaacattt
    29521 ttttcgtatc ttttttacca aaaacaagtg taaatgctat aaatccagtg atagttaaag
    29581 aaaaaatgct aataatggat ggcgcaaata gcaaccctaa caaaccgagt acaacgacaa
    29641 tagtgcccaa tatccctttt ttaagttcta ttttttttcc taaaatgtta ttcatgataa
    29701 taactcctac tattcagttt ttgaagatag agtcagtata catgaagagt taaaataggc
    29761 aatgtttttt tatggatatt tgtattgcta aaaatttatc ttatatattc attttgatta
    29821 tactactttt atttgtaaaa attttctagc aaaaccaagt gcttgcaact gattaagcgt
    29881 acaatttttt agatgttccg agcgtacttc aatcatttca aaggagttgc tacttaaatc
    29941 gatatttttt aaatcagtat caaaaaagct agcattttct aaatcattag tagaaaacat
    30001 tattttagtg agggaacagt ctacaaaatc tgcgcttgat aagacagagt gctcaaaggt
    30061 actttctttt atgtttgaac cagataaatt tacatagtcc attttacaat tctcgaataa
    30121 cgtgttccca atataacttt ccatcaattt agtacctgtt aatttacaat tgacaaattt
    30181 cacgcgatgc aacatgcttt ttgtcaatat acagcctgaa aaatcacagt tttcgaatat
    30241 acagtcggta atctccattc tttgaaaatc attggaagca aaaaatgcat acttgaaaat
    30301 agatttgtaa ataattggat ggcagattat ttggttttcg atgacctctt tttgtgaaag
    30361 gatattatac agttcaaaat cttcgttttc atagatatga aaaaaggatc ctttattcaa
    30421 gcttgcttca ttaactttag gaaaaatcat atttaattac ctccattttc ataaagtcat
    30481 attaacaaaa gctaaaccaa cttacattag cagaaaatga gataggtttt cgcctttttc
    30541 gataacgata gtgtcgctat ctcttgaaac gttcttagaa ttgccctgag agacacaaat
    30601 taggcgtaat tctaataaaa atcttagaat aaaaattttt actgatttaa tttagaatgt
    30661 ttttaaaata aaaatcgttg ccataacagt gtattgaaag gttttaacaa tccagtaatg
    30721 atttaaaaaa atctagatgt agcgtatact ttgtttatag aatgtagtgg gaagaaatat
    30781 atttttggga ggaaatcgtg atgtctaaaa gtgaagaaat agaaaacatg gtactaaaat
    30841 tatatgatct cgttttaaat ccagagatca aagaaaagga acgtatactt ttgatagacg
    30901 caaagacagg cttagaaaaa ggtcagtatt atcctaaagt cataaataat ttggaacgta
    30961 gtttacgtcc gttagcaatc aggggagaat tgtctaaacc agtatcaccg ttttatatgg
    31021 agatatcaac aataggaaaa tttgaaaaag aattaggtcg aggaatggct tcggcaccga
    31081 ttacttttgg gcatttataa aaacgagtta ttcatctcaa tgaattaaaa agtatagtca
    31141 atctcataat gcaggctagg ttcatttatt tgaacagagt ctgtattttt ttattctaaa
    31201 taaagaaaca gatgtttctt tatattgtat tttatccttt aattaacaca aaaacagcta
    31261 gtataaacta ataatgttaa ttaaaagtgt attttatgaa actatcgtta tttattaatg
    31321 ttgcattttt gtaagttaaa aattggaaat atagattgaa aaaatgaaag caataagtaa
    31381 aataaaatga atatatgcga aaaaataatt tacattagga aaaaccttgt aaaaacagtt
    31441 ttttggcata tttatttttt ataaatattt gtaaaataac tatcaatttt ttagttaaaa
    31501 gattggaaat gagtggggga ggaattgaag aaaggatttt tgagagatga tatagtaggt
    31561 tttattttta gttaaaatag gggagataga aaaaatgaaa aagtgtagaa atattatttt
    31621 atcaatgtta tttattatta ctaatataac atcactgatt cctgttcatg tctatgcgga
    31681 tgcaggcaga gatatcagca gtaatgtcac ttcgttgacc gtggacccaa cgaatattac
    31741 agatggagga aacataaaag taaaattctc gtttgacgaa aaaaaacaaa atatccaacc
    31801 aggtgattat ctttggatca attggccaag tgaaggaaat attcgcggtg aaggattcca
    31861 aaaagaaatc ccattaatga ttgaaaataa aaatgttgga actttaacgg tcagaaaaga
    31921 ttctgcgcag gttgtgttta atgaaaatat taaaaaccta gactcagtag aaggttgggg
    31981 gcaatttgag atacaggcca gaaacgtaac cgataccggt gaagaaaata cgggaccatt
    32041 caccgtgacg agcggtgata aaacagctac tgtaaatgtg acaaagccag cttctggttc
    32101 ttcttctagc gttttttatt ataaaacagg agacatgcta cctgaagata ctaagcacat
    32161 tcgatggttt ttgaatatta acaataacgg cacttatgtt gaacaaccag ttaaaatcag
    32221 tgatgaaatc caaagtggac aaagattaga tccaagtacg tttgaaatta atcaaataca
    32281 cttaggtgaa cagaaggttt atagaggaga agagggaatc caacaatttt tacaagattt
    32341 ccccagcgct acatttaatt tttcagtaac tgataattac attgaaataa ctataccaaa
    32401 aaactttgtc aatcttagga aaataatggt gagttataag acgatcatag aaaacccaga
    32461 acaaataaat tttgagaatc attcggaagc ttggtttaaa gaatttaata aacctgctgt
    32521 tgatggagaa agtttcaatc atacagttaa aaatatttca gcttctggtg gagtcaacgg
    32581 tacagttaga ggcgaattga agattttcaa atatatcaat gacacagaga ttggtatccc
    32641 aaatgtgact ttcgaattaa gacgagcaga tgaacagcct attcaaggac agtctagtat
    32701 cctgttgact tcgaatgaac agggagaagc tagtatcaaa ggcctgcaag taggagacta
    32761 tgttgtaaaa gaaaaagaag cacccaattg gattgatttt gatccactaa gttcaaatga
    32821 actcaaattc tccataaacg aaaatgatac agagggagtc agtttaccta tttataacaa
    32881 gaaaaaggtt acgaacatca cagcaactaa aatttggaac ggtggaacca ctccaagacc
    32941 gagtatttat tttaaattat tcagagcaag tacaaacaat tgggaaccag ttcctgatgc
    33001 agaaacaaaa cgattagaca atggaataac ttcagtgact tgggaagata tccaacagta
    33061 tgatgatagt ggaaacgaat atacgtttaa agtgcaagaa gtagatcaaa acggaaatga
    33121 ttatgtccct tcaggttatc aaaaaattga aaacgggcta gtcgttacaa atgagaataa
    33181 agaagtcatt tcagtctcag gacaaaaaac atgggaagac aatgagaacc aagacggcaa
    33241 acgaccgacg acgatcacag taaatctatt agccgacggt caaccgatcc aacacaaaga
    33301 agtaagtgaa aaagatgatt ggcgctatcg tttcacaaat ctgccaaaat acagagacgg
    33361 gcaagaaatc atctatactg taacggaaga caacgtgccc gaatacagta cgacgattga
    33421 cgggtataac atcaaaaaca gttatacacc ggaaaaaaca aatatatcta taccaggtag
    33481 ccatataaca ccgtggaaaa attttccaga gaagacagag agagagaaaa ttagaaatct
    33541 attctctcaa aaattgcaaa tatttgctca tacaggagaa tccagcgaac aaagaagcaa
    33601 ttatattagt aagagagctg tacctaaaca gataacaaat aaatcaaaaa gcattcttcc
    33661 taaaacaagt agtaaaaaat ctagttttgc tgtaatcatt ggtttattga tagtaactat
    33721 ctgtgctggg atattcttac aaaaatataa aaaagtttag ttagaatata atattttttg
    33781 ttaaatatga ctgaaatcaa aagaataaaa ttccgattta taatcgaaaa aa
//
LOCUS       DBNICI010000011        31013 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.011, whole genome shotgun sequence.
ACCESSION   DBNICI010000011 DBNICI010000000
VERSION     DBNICI010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 31013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.011"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(168..3446)
                     /locus_tag="AC1ZCC_000446"
     CDS             complement(168..3446)
                     /locus_tag="AC1ZCC_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIQ1628728.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3717..4262)
                     /locus_tag="AC1ZCC_000447"
     CDS             complement(3717..4262)
                     /locus_tag="AC1ZCC_000447"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIQ1628729.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(4274..4966)
                     /locus_tag="AC1ZCC_000448"
     CDS             complement(4274..4966)
                     /locus_tag="AC1ZCC_000448"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HIQ1628730.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(4985..5269)
                     /gene="macP"
                     /locus_tag="AC1ZCC_000449"
     CDS             complement(4985..5269)
                     /gene="macP"
                     /locus_tag="AC1ZCC_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HIQ1628731.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(5282..5845)
                     /locus_tag="AC1ZCC_000450"
     CDS             complement(5282..5845)
                     /locus_tag="AC1ZCC_000450"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIQ1628732.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            6073..6735
                     /locus_tag="AC1ZCC_000451"
     CDS             6073..6735
                     /locus_tag="AC1ZCC_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HIQ1628733.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            6859..8055
                     /locus_tag="AC1ZCC_000452"
     CDS             6859..8055
                     /locus_tag="AC1ZCC_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HIQ1628734.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(8156..9532)
                     /locus_tag="AC1ZCC_000453"
     CDS             complement(8156..9532)
                     /locus_tag="AC1ZCC_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIQ1628735.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(9569..10435)
                     /locus_tag="AC1ZCC_000454"
     CDS             complement(9569..10435)
                     /locus_tag="AC1ZCC_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HIQ1628736.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(10524..11549)
                     /gene="sapS"
                     /locus_tag="AC1ZCC_000455"
     CDS             complement(10524..11549)
                     /gene="sapS"
                     /locus_tag="AC1ZCC_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SapS"
                     /protein_id="HIQ1628737.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(11567..12241)
                     /gene="sapR"
                     /locus_tag="AC1ZCC_000456"
     CDS             complement(11567..12241)
                     /gene="sapR"
                     /locus_tag="AC1ZCC_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator SapR"
                     /protein_id="HIQ1628738.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(12425..13318)
                     /locus_tag="AC1ZCC_000457"
     CDS             complement(12425..13318)
                     /locus_tag="AC1ZCC_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HIQ1628739.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(13392..15089)
                     /locus_tag="AC1ZCC_000458"
     CDS             complement(13392..15089)
                     /locus_tag="AC1ZCC_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HIQ1628740.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(15082..16395)
                     /locus_tag="AC1ZCC_000459"
     CDS             complement(15082..16395)
                     /locus_tag="AC1ZCC_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HIQ1628741.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(16395..20336)
                     /locus_tag="AC1ZCC_000460"
     CDS             complement(16395..20336)
                     /locus_tag="AC1ZCC_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HIQ1628742.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(20445..20903)
                     /locus_tag="AC1ZCC_000461"
     CDS             complement(20445..20903)
                     /locus_tag="AC1ZCC_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIQ1628743.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(21201..21719)
                     /locus_tag="AC1ZCC_000462"
     CDS             complement(21201..21719)
                     /locus_tag="AC1ZCC_000462"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIQ1628744.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(21721..22337)
                     /locus_tag="AC1ZCC_000463"
                     /pseudo
     CDS             complement(21721..22337)
                     /locus_tag="AC1ZCC_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            complement(22334..23122)
                     /locus_tag="AC1ZCC_000464"
     CDS             complement(22334..23122)
                     /locus_tag="AC1ZCC_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1628745.1"
                     /translation="MTVIGIILIIPLLWMVFTSLKPMEEIVRYPPTFFPEKIVWENYL
                     DTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYGFAKLDFPGKKLMFALVLSTMMI
                     PGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFNIFLMRQFYLSINNELIEAAEID
                     GANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFLGPLLYIQDQEKYTLQIGLQVFQ
                     NQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGMDLTGGSKG"
     gene            complement(23184..24095)
                     /locus_tag="AC1ZCC_000465"
     CDS             complement(23184..24095)
                     /locus_tag="AC1ZCC_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1628746.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(24136..25470)
                     /locus_tag="AC1ZCC_000466"
     CDS             complement(24136..25470)
                     /locus_tag="AC1ZCC_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIQ1628747.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            25678..26508
                     /locus_tag="AC1ZCC_000467"
     CDS             25678..26508
                     /locus_tag="AC1ZCC_000467"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HIQ1628748.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(26752..27001)
                     /locus_tag="AC1ZCC_000468"
                     /pseudo
     CDS             complement(26752..27001)
                     /locus_tag="AC1ZCC_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591496.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(27192..27386)
                     /locus_tag="AC1ZCC_000469"
     CDS             complement(27192..27386)
                     /locus_tag="AC1ZCC_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628749.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(27891..28076)
                     /locus_tag="AC1ZCC_000470"
     CDS             complement(27891..28076)
                     /locus_tag="AC1ZCC_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628750.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(28926..29267)
                     /locus_tag="AC1ZCC_000471"
     CDS             complement(28926..29267)
                     /locus_tag="AC1ZCC_000471"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIQ1628751.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(29267..29497)
                     /gene="mazE"
                     /locus_tag="AC1ZCC_000472"
                     /gene_synonym="pemI"
     CDS             complement(29267..29497)
                     /gene="mazE"
                     /locus_tag="AC1ZCC_000472"
                     /gene_synonym="pemI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemI/MazE family
                     antitoxin"
                     /protein_id="HIQ1628752.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            29784..30122
                     /locus_tag="AC1ZCC_000473"
     CDS             29784..30122
                     /locus_tag="AC1ZCC_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628753.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(30327..30889)
                     /locus_tag="AC1ZCC_000474"
                     /pseudo
     CDS             complement(30327..30889)
                     /locus_tag="AC1ZCC_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
BASE COUNT         9070 a         6621 c         5324 g         9998 t
ORIGIN      
        1 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaagcag caaggagaac
       61 ctcatctata aaaagataga aggaagtgag acatctattg gtctcatttc cttctatttt
      121 tactttcctc agtcaattgt tttacatcat aaaaggagaa attccactta tttttttcgc
      181 tgtttcatga cataaacaac tacaccgatt aacaaaattc ctccagctaa tgctagcaac
      241 gggttattaa attctcccgt ttttgggagt ccttgagtgc catttttttc actttctttt
      301 gctgtcctat ccggatgaga aggttttcca tttggttttc catttggtga ttcatttcct
      361 ggattttctc tttctggttt gtcatttccc gggggcacat cggcagtttc tcgcacagtg
      421 atcgcttttt gctgggtaac agattctgta tgagcagacc atttttcccc atcaaatatt
      481 tccgaacgat aagtgacttc tagcgtatat tctccttttg aaggtgctga aaaaatagcg
      541 gtatgattag aaaattgagc acttgactgt gtttcaacga tttggatctt ttctgggaaa
      601 taacgcatat cttcaaagac aggcgttcct tgatttggtg tgaaagttga cacaaaaact
      661 gtaaaaggct tcagttcaat gatatttcct tctgcattct ccgccacacc atttgcttct
      721 tgtataataa tcggattatt ttgttcattt ggttttgtct gatttggttc ttgtacgatt
      781 gcatactctg gatattccga agacggtgag gtttctccgt caaaaatagc acctacagat
      841 aatggaagac catccactct ttccttttct gtttgacgat tccccggttt aatctgcatc
      901 agtccatgaa catggatact tccattttca tagcgcgtaa caggctgctg accttctttc
      961 gaacatgtat ttagagactc gatccaatct tttgatatgg tatgtccaag agtattttct
     1021 gattgtttct ctcgttcatt ccaatagtag ttcaacgagt ggtagatttg atcttcgttt
     1081 tgattcaatg ggcggatctg ctctctcatc tcaagattct ctgttcggaa agataagata
     1141 ccagaagcat ggtagtctgt gtttcctgct gaagaggtat aaagagccac attatatgaa
     1201 ccgttattaa aacttgtact gctattgacg ataatatctg ttccgctatt ggaatcgatt
     1261 cctttcgcaa gattttcgaa agacaaacta ttgatcaatt catgagggcc cgttaaagaa
     1321 gaaccgccca ttttgaatcc attaccgtta ccttctccct caattcctgg tacccagccg
     1381 tttcgataag caactgagtt tttgatgatt acttttccga tcgaacctgt ttcattttta
     1441 gcaaaaaggt cccaaccgtc atctgcatta tgatacgcga tacaaccgtc aaatacattt
     1501 ccttctccta ttgtcagttt ggccgcaaat ccatcggcat cttcaaatcc tggatcggca
     1561 ttttcaaaag atgtacagtt cttgactaag ttatgcgcgg gccatgcttc gaaaggatca
     1621 tttgagctac cgctgatttg gattcctgta tttccattcc gataagcatg aatctcttcg
     1681 atcaaattat gatttccaga aatctgtaat cctttttgca tagctgcact atttgtaatg
     1741 tcaaaccctc gtaaacccca gtagtgactg aacagtgtca tcccatttcc agttccttta
     1801 aaatcaagta caggacgctt gttttctgga tttttctctg caatcaactg aatcgggttt
     1861 tctcttgtcc cgttaacgcc ttttggaatc gtcaatcctc tttcgaagga ataagttcca
     1921 ggcgccatga cgattgtctg cccagcatat gcgtatctca atgcttgtgt cagagataat
     1981 ggattttgtc tgcttccgtt tcctgcatct gacccttctg gtgttacata aactgtcgaa
     2041 tatttaggtt tacggtagtc cactgtatga agaatctctt ttgtttcata actagtcatc
     2101 tctacgtatt cttctggcgg atagttttta tctggcgtaa aactgaaagt aaattcgttc
     2161 gttccttcca tcagttttgt ctgataatca aattcccgat tcgcagtgat agccaagttt
     2221 tttgcgttct taagcacctc tcctgttgct ttatttttaa ttgtcaataa tccgtcagca
     2281 tttgctctaa aggacatagt atgatcttgg tttcctgaat gattactaga ggtcacagtg
     2341 taaatcggat caatcaagcg agtaggtctt tcttgcccag gaggatctgt cgcaggatct
     2401 gagtaagcaa cttcgattcc ttctaccttg atcgtcatat tccttgctgc agcaaatcct
     2461 gcataaataa ccgattcatc tgaagcgtat aatttttccc agtcatacat gatgtcagaa
     2521 gccatttctt ctcctgtttc aggatcgtaa taatatgcct ctaaaccagt attcgttttt
     2581 ttcatctcta cgtccagttt agtaatcgag ttcgtggccg gaaacagatt cccatgaccg
     2641 tttccgaata tattataaga acctgtttct ttttccaaga accctgctga agtctctaaa
     2701 ggtgttgttt ccactgagac actcccagga gctggcgcag cttgcggatc ttcagatgat
     2761 attcctgtga ttgcccgtgt ccccaatcca agtcgcatcg tatatttgct gccgctcgtg
     2821 ttgaccactt cttgtctatc cgagtcccag acatattcga tccgactgcc tagtatggca
     2881 aatgaatttg aataaaatgc ctgcccaaac aaatgatttt ttggtactgc gtctcttacc
     2941 attaaagcaa atccttcttg ggcattcgta taggtccaag attctatttc gatcgtcgcc
     3001 cgcatcctaa agttgatgtc tttggggatt gcttgataat aataggtcat tccatctgat
     3061 ccgcttgact ggaattttcc gccgttattc gtggaagaga gccgcacgcc gtcttctaat
     3121 ggttcaatgg catacctact agcacttgtg ctttctccga aaacaacccc tttccactct
     3181 tcatttgctt cttcagattg cttttttgtc ttttcatttt ctgattcttt catcatagga
     3241 tcatttcctt cttccgtatc catatcttca ctagttcttg gtatttcctc cattgttgac
     3301 atcccttcaa tgttcacgtg tgcattttct tcgttcacag actgttgttc gttagcagca
     3361 acttcaacga cagaaattaa aaccggggta ctgagcataa ggagattcat cattagcgca
     3421 ctgtatttta tacgtttttt taacaagtgt attcctcccg taacaaatta gaaagcgttt
     3481 acttaaagta ttacctaatt tatttttact gtcaataaaa aacaaaatta taataagtgt
     3541 attttataaa aagaaaaaat ataacttaag ttgttgataa taaagagatt tattaacgga
     3601 aaaagaataa gtttttttaa aataacgtat aaaaaagagc ataaaacaaa gtcgtttgtt
     3661 ttatgctctt cttttattat tttgtgcttt ttattttatc aaatcacttt gtttttttat
     3721 aaaatttctg cgcctagtgt ggactcaaaa tgtctcaatg cccattcatg tccaacggga
     3781 tcgaaacttg ccacggaatc tttgaaaata tggagtttgt atcctagatt atacgcatcg
     3841 actgctgtat gcaacacaca gatatctgta catacgccgg tcaaataaat atctgtgatc
     3901 tggcgttctc gcaaacggat gtctagatct gttccactaa aagcggaata atgccgtttg
     3961 tctatccaat aaacattttc ctcttgctca tagtcttggt ataaactttt taaagagccg
     4021 tacaattccc gtccacccgt tcctatcaca ttatgcggag gaaataagcg gttctccgga
     4081 tgaaaggaat cttttggatc atgcgcatca atggcaaaaa cgacaaaatc cttttgatca
     4141 ataaacttct ttgtatgagc agtcagcttt ttttcaatta cttgtcctgc tgtcccagta
     4201 gtcagctttc catcctctgc aacaaaatca tatgtgtagt caatcgagat caatgcgttc
     4261 atcttagtcc tccttatacg agatgtttga caaattcgat caccatttct gctgatcgtt
     4321 tgcctgcgtc ttcgataaat tcgtcaaagc tttgtgtcgc cgagtgatct gctgtgtcgc
     4381 tcattgcacg tacgatcaaa aatgggatat tgaactgacg agctgtttgt ccaacagcgg
     4441 ctccttccat ttcgcaagct aatgcttcag ggaaattcgt taagatttct ttgattttat
     4501 ctggcgagtc gacaaatgta tcacctgtaa cgatcaatcc ttcttttgcg ttcaaattcg
     4561 ttgcttttgc tgctttaacc atttcactgc gcaaatatgt gcttgcttca taatatagag
     4621 gcattcctgg taattgtcct gggttgtagc caaatcctgt tgcatctgca tcaaaataag
     4681 ctactttgtc tgatataacg atgtcaccta cttgtaagcc ttcaccgatt ccgccagctg
     4741 aacctgtatt gatcaccatg ttcacgccgt attgttggat caacagactt gtggtaatag
     4801 atgctaagac ttttcctata ccggaacgta cgacgatcac ttcgtggtta cctaatgaac
     4861 ctgaaataaa taaagcgcct gcgcgttccc atgataatgg ttcgcttagg ttctctctca
     4921 aaatttttac ttcttcttcc attgcgccga taataccgat tttcatcttt ttaattcctt
     4981 ctttctatat aaatgcgatt gctaaaaaaa ccacacataa cattaggata acaataataa
     5041 gagatttcat caaaaaagag ttccatttgg tcgtcttttc ccgttcgctg atccttgttt
     5101 tcgtgattgg tttattcttt ttgctttctt tacgataaaa gctggcaatc tttttttctt
     5161 cttgctgata agctgtttct gcttttcgct gtttttttaa tgactcgctt gcttgtgctt
     5221 gttgtcgctt gcgaagttcg cttcgcgtaa taagcggtcc tttggccata agccgatccc
     5281 tctactttcc tttggtcaat agttcccagt attgaatcgc ataaatcgtc tttgcatcac
     5341 aaatcaactg atctttcatc gcttgttgcg cctcttcaag agttagatga tacaactcca
     5401 gtacttcgtc ttcatcttgt gctaaaggat tctcgacctt ctctacccct tgtgcatgat
     5461 aaatatgaag gacttcattt gcaaaacctg gtgaaagata catcgaagta agatggctca
     5521 gcgacttcgc acgatagcca gtttcttctt ctaattctct agctgctgtc atttcgggat
     5581 tttgtccttc tcctggatcg atttttccag ctggaatctc caaaatcact ttttctaaag
     5641 gttttcgaaa ttgtttgaca agaagcagac gatcttgttc gtcaaaagca atgatcccga
     5701 caccgccagg atgaaatacc agttctctct tcgaagttcc tccatctggc aaacgaactt
     5761 cgtctacagc aacatcaata atttttccgc tatagatttc tttgcgactg attgtttttt
     5821 cttcaaattg ctcattgttc agcatatctt ctcctgcttt ctttgtaatc ataactgctt
     5881 ttttttcatt tttcaatttt tctcaaaaac tccttattag tttaccatag ataaacctta
     5941 gattttctct aaatatagaa aatccttctt tagtccgata ctctttctag tagtccgtat
     6001 gctaaaataa gtatgtaatc gaataacgaa aagaattcaa tactcaacga taaatgacat
     6061 ggagggttta ttatgataaa acgaaattgg ccgtggcttc tagcattggt cttgctattt
     6121 ctctttgtcc gaaacaatag tttttctttt gttctaggac ttttattgat aggtgtagtt
     6181 ttagttttgt ggggactctt tggttcgcgc aggaaaaagg gaaaacaaga agaaattcct
     6241 gcactgactg acgaacttga aactcactat gaaaaaagcg gtatgacagc gaatgaaatt
     6301 tcattttttc gacaaacaat gaatcaaaca aaaaatgaga tcaatcaatt gcaaatgaat
     6361 atgcagcaga cagccaaact taaagcaatc gatctccgcc atgatccagt caaagcagcg
     6421 aaagctttat tcaaagaatt ggtgaaagaa ccaaaccgac tgcatgaagc aagtcagttt
     6481 ctttatacgc atctgccgaa tctagttgat ttgacaaata agtataatga gatcaatgac
     6541 catgagatca aaaacaaaca gacctatgaa aaactagaag aaagcgcgca gatcatcgac
     6601 cagctgtcag ctttaatcgc acaagattat caaaaattcg ttgctgagga tctagatgat
     6661 atcgacgtag agatatctgt tgcgaaacag agcttaaagc gtgataataa atatgaacca
     6721 gaacaaaagg attaactctt ttgttcataa aagaagactc ggaacattca gatgatgtct
     6781 ggctgtttcg agtacttttt ctaaagtaga gacattcatg taagaatcga gaacagattc
     6841 aacaaggagg aattttttat ggatcataaa ccgactgaaa caaaagatgt tacaccagtt
     6901 aacgatacac ttgaagattt attgaacaac ccgttttcaa ctccaacaga tacgctgacc
     6961 acttcacaac aagcggagat tgatcagctc caagagcaac agacagctgc tcgtttaatt
     7021 gacaaactac cggcggaacg ccaagaacaa gctaaacagc tagcagcaaa aatcgatgct
     7081 aatgatgcac agtctgtcat cagctacgga tcggctgccc aagcaaaatt aagtgaattc
     7141 tcacaatcga tgttgaatca tgtgcaagca caagatattg gtcctgtagg agattcctta
     7201 actgaactga tgtatcgtct ccaagaagcc aatcctgacg aactgcgtgc cggagaaggg
     7261 aacatcttcc agcgtgtctt cgggaaggtc aaacaatcga tctatgaagt gacagcaaaa
     7321 tatcaaaaga ttggtgcaca aattgacaag atttcagtga aattagataa agaaaaagac
     7381 ggtctgctaa aagataatct gatgttagaa cagctttacc agaaaaacaa agactacttt
     7441 gatgcactga atatttatat cgctgctgga gaattgaaaa tggaagaact tcaaacaacg
     7501 atcattccag aagcgatgaa aagagcagaa gaaactggcg atcagatgga tgttcagatt
     7561 gccaatgatt acacgcaatt cctagatcgt ctggataaac gtacgcatga tcttcgctta
     7621 gctagacaga tcacgatcca gcaagcacca caaatccgtt tgatccaaaa cacaaatcag
     7681 gccttggctg aaaagatcca agcatctatc gctacagcca ttccgctgtg gaaaaaccaa
     7741 gtcgtgatcg cattgacctt gcttcgtcaa aaagatgcag taactgctca acgccaagtc
     7801 tctgaaacaa caaacgactt attgaagaaa aattcagaaa tgctgaaaat ctcagccatc
     7861 gaaacagcga aagaaaatga acgtggaatc gtggatatcg agacccttca aaccactcaa
     7921 aatgatttga tcgaaacaat ccaagagaca ttgcgtatcc aaaaagaagg aaaagaaaaa
     7981 cgtcgtcatg cagaagtaga gcttggacac atggaagaag atctgaaaca aaaactgttg
     8041 gatttaaccc aatagatcac ctttcgcttg ttactaaaac aaacgcccaa caacagttta
     8101 ttacaactgt tgatgggcgt ttttcttatc tttcttctaa cttctccatt ggagtctatt
     8161 tctcattcat cttgctgtgt ccatagccat agccaaaata aatgatcagt ccaataacca
     8221 ttacgatacc gaacacgatc catgtcacag tttgcagacg aagcatcaat acaatacaag
     8281 tcaagatgga gacgattggc aatagcggaa cgaatggcac tttgaattct ccagcttttg
     8341 gtttgccaaa catttttctc aacttcagta agcctagact aacaagcatc aaataggcga
     8401 gcgccatgat attcgttagt tccgctaata cattcaatgg gaaaaatccg gcaaagaata
     8461 cagctgccac accagcggca taagtcgctt ttttcggtgt acgatgcttt tgatcgattt
     8521 ctgtcataaa cttaggaagt aagccatcct tgctgacagc aaacagtaaa cgagcaagtg
     8581 aatacatcat cgagatcgtt accgtcagta gcgttaaaat tgcccctaca gcgatcaccg
     8641 cgcccactgt tccatgtcct acatacctca ttgcaaaagc cactgggtca ttgactccta
     8701 gttcagtata cggaacgatt cctgtcaaga tcagcgtcac gatgatgtaa aggacagtag
     8761 caatcagtat cgaaccaatg attcctcttg gcacatcttt ttgcgggttt ttgacttcct
     8821 cggctgccat tgcaacggca tcaaatccca agaaagcaaa aaacacaaga gctgcaccat
     8881 ctagcacacc ggaaaaacca aaaggcatga atggctgcca gttatcaggt ttaacgaaaa
     8941 atattcctac aatgataaac aacgcgatga taccaaattt gacatatacc atggcattat
     9001 tcaaacgtaa tgctttcttg gcttcaagcg acacgatgta agtgacgaat atcactacga
     9061 gcaccgcaat caagtcaatg tatgtaccat tttccggatt gtacccgcca gttaatgctt
     9121 gtggaagttc ggcccctaaa cttttcaaaa aaccttgcat atagccagac catccggaag
     9181 caacagagga aaccgctagt aaaaattccc cgatcaacaa ccaccctgtc agccaggcag
     9241 caaattcacc aaatacaaca tacagatagg catatggccc accaatcact ggcactcgcg
     9301 aagcaaattc tgcgaaactt agtccagaca aaatgacaac gattgcagct aagacaaatg
     9361 aaagtgacag tgctggacca gcattttgat tggccgcaac ccctgtcacc acaaatattc
     9421 ccgtaccgat gatcgcacca attccaagca tgatcaaatc agacgttttt agttcttttt
     9481 tcatctcact cgtatgattg agattgatct ctttcttacg aaacaaatcc attgaagttc
     9541 ctccccattt taggttggaa aacagcgatt aaactgtttt catataccct aaaaggatac
     9601 cacctaaaat gactaaaagg caaccaacaa taacataaat gagctctttt ttcgtttttc
     9661 tttcgccaag gatgaaaatc ccacctaaag tagagataat gatccccatt tgtgaaagtg
     9721 agaagccaac agctaaacca agtgatttga ctgttagaag catgcagatg tttcctaagc
     9781 cccataataa tccacagatc atgtttttcc acacaaactg atcgacttta actttacgga
     9841 aagcaaagaa gcttgcgcct aagatcattc cgatactttg aggtaagata attgacatcg
     9901 catccaaatt cgcccagttg acgatgatgg tatatacacc ataaccaatc gtagagaaga
     9961 tcaatgctct aaaaccctta ccaaaatcaa gcattttatt atctgtatct atttttcctt
    10021 cgtcatcttg tcgggcagtc aaatacgcac cgcttacaag aagagctaaa gcaataatac
    10081 cgtatacata atctttcgtt tgtgtccatt catggaagaa aacggctccc gcaatcgtat
    10141 tcaaaattaa ttgaaaacca gtagacaatg gtagccctac agaaacaccc atgtacttca
    10201 ttccatggaa ctgtccattt tgtccaacac tccaagctaa gccggataaa aatccgatca
    10261 agaaaatcca aggggtgatt acaggactaa caacgaaaaa gacaactaaa gaaaacagaa
    10321 atgcaccgat cgtcattcct agtgtttgtt gattggcatc gccaccaatt ttaccactta
    10381 ctaaaccaat actaccccat gccaacatag ggactaatgc tattaaaatt tccataacca
    10441 atctcctttt atttcacatg ataagaaact tatcagcgaa aacgttaaca acacaagcac
    10501 tttttttcgt ttctaacgat tttctaacgt ttttcattat aaaaatttaa gattgggaaa
    10561 aataaagtca ttgttgtccc ttttccttca gttgattcag ccgtcaaatg gatacctaat
    10621 ttttcggcca gattttttgc taaataaagt cctaatcctg ttgaatgtgt tttgctaagt
    10681 cgaccatttc tcccagtaaa tcccttatca aagatccgtt tcaagtcttc cgctgggata
    10741 ccgatccctg aatctttaac tgaaaggctg actccttgcg cttccttttc tatgctcaca
    10801 atgatgtttc cgtgatccgg cgtatatttg atggcattac taagcaattg gttgaagata
    10861 aaagcgatcc atttagcatc ggtcaaaaca gtatgatcct caccaacgat ctcgtagcga
    10921 atattttttt ggataaaata atttgcctga ctgcgtatca caggctgaat gattcctttt
    10981 agtccatatt cttggatgag gtagtcccgc gagaaactgt cgagtcttgc ataatacaaa
    11041 acttgttcta cgtattcatc gatttttgat aattcatttt ctagtaatgt atacttttga
    11101 tcgtcaatat cgaactcgat cgaacgaact agcaatttag aagcggaaag tggcactttg
    11161 atttcatgga cccatgagtc aatataatct ttttggtctt gttgattact gattgctgat
    11221 tgcattactt cttggtgttc acggatcagc tgattgatat attcttctac cagtttttct
    11281 tcttctgtcc tcccaccact caaataattt tgcaaagggg aagttttttg tatgtcggct
    11341 aattttcccc accatcttct cttgctcaag taaccaccca gcagataaaa aatcagaaag
    11401 acgccctcaa tcaaagctaa ataagcaatt gtgctcaaat caactttgat gtttggcgcc
    11461 agccacatca caaagatcgt caatgcaata aaaaacagcc atcccaagat caatgtccac
    11521 tgatctttca aatatttaaa aaaattcatc ttaaccgctc atcctttcag ggaacgatat
    11581 atccctgtcc tacttttgtt tcgatatatc cttggatacc tgcctgttcg atttttcggc
    11641 gtaaccgatt gatgttgact gtcaatgtat tgtcatctac aaatcgttcg tcatcccaca
    11701 gtgctcgcag caatttttcc cgacttaaga tctttccatt gtgcttcatc aaaataaata
    11761 atagacgata ttcatttttg ctcaaatcaa tgatctctcc gttgatttcc atacttccgt
    11821 tgtcaacatt caacgtaatg ccattgtggc tcatcatttc actagataat tctgtgtagt
    11881 tgtaagaacg gcgaagtaat gcattgattt ttgcaatcag tacatcgatt tggaaatgtt
    11941 ttgtcacaaa atcatctgcc cccatgttca tagccatgat catatccata ttcgtattcc
    12001 tgctggagat aaaaatgatc ggtactttcg atacttcacg tattttttga caccaataat
    12061 agccatccaa aacgggaaga ttaatatcta gtaatacaag ctgtggttct tctctttcaa
    12121 agtctgagaa cacttgattg aaatcggtcg ttccaaaggt ttcaaattgc catttctcaa
    12181 gttcctccat gatcaattgt cgaatgatct cttcgtcttc gactaccatg atttttgcca
    12241 taaaaaatcc ctccatcttt cttcttgcta ctacaatatc aaagaagccc ttgtgaaaca
    12301 agttggctta cctggaaaat gtcattttag caaacgttaa aaaaaactgg aaatatgcga
    12361 tttaatcgcg tttgtctctt ccaagcagct taaatttatt atgttattct taataattgg
    12421 tctattatct gttatttttc tttcgttgtt tttgtctttt aaattttcga taacaccaat
    12481 aaccgaaaag cgttagcact agcaatatac ctagaaggag tataagtaat cgataaagat
    12541 ggtatttttt tgtctgttct atctttgaag agacttcttc tgtaaaggga actcgtcttc
    12601 ctgtcactag taatcggtga gtgttcacgg tgtaaggtgt acaagtgata agtgtgacta
    12661 gatcctgctt ttctctaatt gctaatgtat ttgtttttgt tggaaggaca acaattttct
    12721 caacgacttc ataagcgagc atccgaccat acacttcaag atagaatcgg tcatcttgct
    12781 ccatttcttc aagatccgta aagagttttt tctcagcaat accgctatgg gcggtaatca
    12841 cggaatgtgt actctctcct ccagttggaa aagaggtgcc ttgcaggact gtcgctcctc
    12901 tatctaacaa aagatcattc gtttcatcaa ataaaggcaa gctaacattg attttcggaa
    12961 tatagatggc tccgatcata tgttgttcgt aatattcttt tcctggattt tggacatttc
    13021 taaccgcttg ctcaaaagga tctttgactt gtcccattcc tgggattact gatgtatgag
    13081 caagcgtctg atttttcttt tcatgcactg ctaattgttg ctcatgtttt tcttctttat
    13141 cgcgttcaag ctgcttttga taattctcga tccttctttg atccaaataa ttgctgagag
    13201 catctgctaa aaatggatat gtaaaaactg cagctccggc aagaaacatc actgccataa
    13261 ataattgtaa taaaagactg atttgggttt gaggtctatt acctattttc tttttcattt
    13321 tcttcacctt cttatttaaa aaaactgcag caacctttat aaaaccgatt gctgcagctt
    13381 atttttgttg cttatacttc agacttcatt ttagatttac ggtaccagct gtacgctcca
    13441 atcatcaaac ttattccgat tagtaagaat gccaagagcc cgttcccacc agtagcagga
    13501 agtgacccgc gtttcatatt cggtactctt tctggccctg gattttctac tcctacgatc
    13561 tgaatttctt cattatagct tccataagag atgataaatt cagtaaaggc tgcctcacct
    13621 gttggaagta cataaccttc tggcgccatg gtctctttca tttgataggt tccataatct
    13681 aacccttgaa cctcaaagta tccatttcca tctgcgacaa attctgttgc ttgtccgctt
    13741 gttgcccact caattctatc tgctcctgat tcataaacag ctaaaggcga ctccccttta
    13801 taaaatattg cgtattcaga acggtcttca ttccacaatt cgaatcttgc atctttaagc
    13861 aattcactgc ttgatgcatc atatttttca aactttcttc cacctgttgt gactttctct
    13921 ggcggctcta ctccttcttc ataatcaaat tcatcatttc gtccgatttc aataaccgct
    13981 gtgttgttat ggaaatcatc cgcttctagt tctgtggaaa ccttcaaatt atacgtgatc
    14041 gttaaatctc ctccacgtgc atctgctaat ctacctaaag tatctacttt ggctactcca
    14101 ttttcttctg tcaaaatcgt cacgacaaac ccgttgctta ctacttctac tatataatct
    14161 tgtccttttg ttaaaatcgt ctcgccggct cgcacttcta tttcctgatc ataataagtt
    14221 agaccaactg taggcgtatc tgtgattttg taatttttga taacagcgct tccgttttca
    14281 aggacagaat cgatgaaata aggaataggt gctgtgatcg tgtaagaagc cgtttttcct
    14341 ctttctagat caatataaga aatcgtttct tgttctccag cttcattaat aatcgttact
    14401 tgtctaaggt cgctttcctc tacattcatc tgtttatctg cttctctgat ttcgtttttc
    14461 ggatacaaat gcacatctgt attgtatgtg tctttccatg tagaaccatc ccacacgcca
    14521 tccaccatat caggcatcat cactggcatc ataacaacca ttggagacgc gattctggaa
    14581 atatgagctg gcgaataagt ttctaagaaa agatagactt tatctctaga agaaggtgct
    14641 tcttccgtca cttggacggt agagaatacc gcttctccag cttcattcgt tgtttgtttg
    14701 tcaatacttg tcagtcgata ttttaaaaac caactttctg tgtgccgttt ttgaatccat
    14761 tcaatcgctt cggcagaatt aagaccgtct tctcgttccg gtgttaaagg attatctcga
    14821 atcaaatcgt aatatacttc cgttacgtca aacaaatcaa aatcgactcc tggaatattt
    14881 tcttcaccaa aatccgtaga aaccaatccg ctattcggtc gttcttccgg aacagatgag
    14941 aaaccttttt tgtgtaatgt aatcgtcact tcacttggtt tttgtgcgac ttcttcggct
    15001 ttgactagtt ttccgttccc gaaaatccct attcccgcag taacgagtaa cagtacactc
    15061 attcccgtga caatcatttt tttattcatt tttttctccc cgttcttttt ttattaataa
    15121 aacagcacct aacaataaaa gaattcctag taaagaaatc aacgttcttg cttcattcgt
    15181 ttgcgggaat acggaccatt ttcctgattg acctggattg cttggaccac tcggctcttt
    15241 cggtttgtcc gttgtttctt ttacggtctc gttataaaca cgtggtgttg cggattcata
    15301 ggcagagact ggaactttcc cggattctaa ttcagccatc tcctgtccgt taactactac
    15361 atattgtggt tggccttttg catccaccca aaattcaggg atgatgacct cgattctctt
    15421 acttgcttca tccatctcgt aaccttccac accttttaat tcttcaaaat aataggtgcc
    15481 agaaggcaag aaacgttgtc ctgtcgtaac aaggccctct ttatccgaaa tgaatttgct
    15541 tacatttttg tcattcaaag gatcattgtc tgctggtttg atccattgat ttttcaagtc
    15601 attagcagga gccatatcta aataatattt ccccccttct atcatctgat atagagcaaa
    15661 aacagcacct tctaatggtt ttcctttttc agttgttcct ctttgacgtc catacttgaa
    15721 gaagtatgga tctcttagat aaccgacatt tttaggatag atatgaatct cttgcagttc
    15781 ctcggttgat tctgtcgggt gataaattgg caaaatagca gcaattggga tagctgtctg
    15841 atcgatatca acatccaatc caacttctgg atcaatcgat tcttcgaaaa tcaaataaac
    15901 tgcatcccga tcttggtttt tcttaggtgc tttgatacgt gcaatcccgt ctcctaatcc
    15961 gccaaccgta tctttcccag ttgttaccga agtccctatt tggacaagcc tttcttctgt
    16021 gattagctga cggatattgt ttctgttcgt ttcagcgatt tgctggacga attctttagg
    16081 agtcatgttt tcagcagctt ttttttcatg ataataatcg gtcatgtcgt aaatggcaaa
    16141 tgtcgctcca tttagtggca ccgtctgtga aatgatatct gctgtttccc ctgcttcctc
    16201 agagatagcc aagccatcat tttcatggta ttcaaattga tcgttataat cgatatctcg
    16261 aatcatccgt ttatgcaaca cgaattccac gacatcttcc cctgattctt ctgccgacat
    16321 cgcatgtgga agaaggactg ctgcaagcgg tagtaaaaca agtaattgaa gcatcaaaca
    16381 caacagcttt ttcattacct tgctccctcc tttttagatt gtctgacaaa caaaccatag
    16441 gctgctcctg caagcgccat gaagaatgca gcaagcagga tatagaataa tcgtccggat
    16501 cctcctgtag caggcaacgg attttcagga tcgttcccga cactaagctg taatctgatt
    16561 cgatttgtct ccaaaagttc atattctaac tcttgctgta gatcaggcgt ctctgcttct
    16621 tcgtttggat aatacgaaat ctcaactttt agtgtacccg cttcttttcc tggaactacc
    16681 cgatcatttt ctatgtcagg ttcttctcga taactaattt ctaaaataaa gtaacctgga
    16741 agttttctat agctgtcagg tgacttcact tccttgatcc gatagacccc ttccaacaag
    16801 tctgaagcat tgaataggga agaatcgttt ggatctggtg ccaagcctcc ctcgatgatt
    16861 tcatactctc ctgcactatt ttgtttctca attgaaaatt ctgcgccttc aagacgtcgg
    16921 ctattcatac tgtcaatttt caaaagttcc aaatcgattg gcgctagttc atcttctaag
    16981 gttacttgta gtacaccatc gatgatttca tagccttccg gcaagggagc agtttcgtct
    17041 tcgatgaccg tatcttcaat gataaactta ccttcttcat tcagtgcaaa ctggaacact
    17101 tgatcagata gccgataccc atttggtgcg acagattccg agacctcata ttcaccaata
    17161 gacagatgca gaggttggtc gtaatttaaa tcgacatact tttctactcc tacggcacga
    17221 gaatagaagt atccgatccc aggcaaacgt ttcgattctt ctggttctaa cggattacca
    17281 gtgtatgctc gatacagcgg gaaggaagaa tcccccactt tcctgatatc gaaacctgca
    17341 ccatctaatg tatggccttc atggtctaat tttctaactc ggatctcccg ataatcaaac
    17401 gtattgtaag cagtgaatgc tacccataaa cgatcctctt catctgttgt atacgtgaga
    17461 tattgccaat tttcttcacc tgtttgcttg agttggatag tccctatagc attatcgtaa
    17521 agtcgcaaga tccatgtgtc attttcatca ggttctttga aaccttctgg gttctggctc
    17581 tcaatgattt tgtattctgc atagttggta gtttcttcac tatccttata aacaaatgaa
    17641 tctggctctc cgattggatg aataactttc tcaccatcca cgattacata tttcccgtct
    17701 tcatctactg aatagaacgt gagtctccct ttctcttcct ctacttcgtt cacaccagaa
    17761 agccctttcg ctactggata tccgatttcc agtccgttac catctgtttc ataaagttca
    17821 aactctgcgc cattgattgg catattactg ccttcaatta gtttattgat attcatgtgg
    17881 attggcttga attcatttcg catttccagt ttgacataca actcatcata ctctgtgcct
    17941 tctatgttag gctcttgtac aacagaaagc ttgtgatatt ctccttcata ataggaattc
    18001 ggatctttac cttccggtcc gccgactctc gaaccagatg tatattcatc tacaagctcg
    18061 tagtgaccta acatctcatc ttcgttggct gcatattttt tgatttggaa ttgatacgtc
    18121 tcatctacta actgataacg tggtggtcca gcgatctctc tgatccaaaa tatgtcgctg
    18181 actgctccag tttcatctcc taaaatcggc atcttcgttt caaagatcgc tattccgtcc
    18241 gtggtagttt tacttgtttc gattggttcg gcttccccac tctctggatt ttggtcatat
    18301 aatgcaaatt cgactccagc caacggctcg ccattttgac ccttcttgct gacttggaaa
    18361 ttacggcctt tatgttcgta acgaatggaa gaagatggga aatacatccg tccagtagtg
    18421 atatcattct cataatcctc caggttctga atataggcca tattgttcac tgtgcaaaaa
    18481 tttccgtctt gagctcccgc atcaagtttt acttggtact tgatcctgac catttcccct
    18541 tcacctaaag aaatcggaga aaatttataa gcttggtttt cccgtccgac atacgttgtc
    18601 ggagtcgcca ctacttctgg tggggcttgt ccatgacgtt cttcatccca tatttctggc
    18661 tcttggtact tcatgccgtt ttctcctgga tctagatggt aaaactgaat atctgccgat
    18721 ctgccagctc caaatggtga gacattaaaa ccagtttcat cataaagtgt ggcttgctgg
    18781 atcgttctct tgaaggtaga agagatatcc tccaaggctg taacaatgtc attcttgttg
    18841 ttcgcattgt aatatcgcgg tgttacgcca tcttcttcta aatcagcaat atttttcaat
    18901 acatttcgtc caatagcatc tagtcgttgt ctcccgctaa cgtttccatc gattcctaaa
    18961 ccaatggaga agatacttgg cgggttctct tgatacttgt ttttcagcca atgatgataa
    19021 gcaactgtgt tgatcgcaga tgacttttct tgcgaagtag gactgccatt cgtattgtta
    19081 ttagaagaac catcaccaac ccagtgagtc gctttattat ctccttcttt tacctctcca
    19141 taacgatagt gtacatttac ttcatcttct ggacggttaa agtcagatgc ataggtaact
    19201 ggatgagtaa accctgcatt agaagtattc ccagaaaact cctcaaaatt tctgaacaaa
    19261 acgtcattat cttcagcaat cttatcttcc atcacagtcc agttgctcca agctccatta
    19321 gtgctcgtac gataacttga ctgaatcggt gcataactga aggtcggtgt cccatctccc
    19381 acaacgatta ggattttttc tggatttcta ttttctccgc cattgtcttt atacaatgtt
    19441 tcatagccat ttttaagccc tagtgtaagc ggagttcctc cgattggtgt acgatttaat
    19501 gctgtattct cacttcccgt caaacgattg atatcatttg tcaatggaaa gatgtccgaa
    19561 ctgatttgct catggttatt gatagctgta ctattatggt aaaaattcac cataccgatt
    19621 cgaatatcca gactcgggtc agagagcaga tctttactca tttcgtttac tgcctcaatc
    19681 aacgcagcat ttttagtttg actattggtc ccagcggtta attcactcat actcgctgac
    19741 ttatctagaa caaaaacgat atcgattgga ctgatttcct ttttctcgcc cccgatgaca
    19801 tctagataga cattgtaaag ggttgttgca tcttcaggat gtagatcaga tgctgtgctg
    19861 cttggcagaa caggttctac ccattttttg atgtacgccc cgttataatc caagtaactg
    19921 ttttcaaaat tccgttcatt tccccaaagc tgaacggttt gagctggaac gtttctatct
    19981 tcttcgcctg cagttgatcc attattagta gcatagtcgt aatttcgcca gttcgctccc
    20041 ccgtttgtat aatcactatc atcgatcaag ttctctccgt catatggcga gcctttgtct
    20101 ccttgatagc gttcattgct ttgtgtccaa tttcctaaca cttttgtata gtcattcgtt
    20161 gtaaacttcg ggtagcgatg ctgttcgttt tcaacttctc cgctaggata gatatattca
    20221 taggggtctt gcgtagctgt tggcccaaga cgctccaaaa taccgttgaa taactggcgt
    20281 tgaatgcgct ggagtgcttg accgctgggt acgattggtt cgtccagctt ttccatctca
    20341 ttttcttcgc tacgttgaat ttcagcggaa tcttctgtat cagtttcatt ttcttgtaat
    20401 tcttggttat ctattctttc atcaaccacc acttgaagag gtagttaccc ttcgttcaca
    20461 tcggggatct tttctgtttc aaaaatcaat gtgccggttt ctgcttcttt tgaataaact
    20521 tttccgacat accattcttg accatcgatc gtctcgactt ttaggtcttc tccatcttca
    20581 tcataggagg atagacctgt tccacgaacg ttttttacgg taccaatccc ggcggctgcc
    20641 gcatcgattg ctaaacgtat tctaccttca ttggcacttt cctttttttc aaaagccaga
    20701 tgccattcaa gggtttcttc tttttcaata tacttccctt caatttttgc atagtcctca
    20761 tctaagaata cctcatcctc ctttgtttct gttgactcag cagctacatc cagggtcgtc
    20821 atatgataga ctgactggaa atatggtcca aatagcaaac tgacagttac taactgcaaa
    20881 agcttcaatt ttaacccttt cacatcctta tactcctttc aaaaatattt gtcttctttc
    20941 acaacaatca acaaaacaat gcccatctaa ttataattta atttaatttt aattttaaat
    21001 caaatgatat cctttttagt taaaaaacac caattataca acatttatta ttaaaattaa
    21061 attataaaat tttataacgt tctcatttaa caaaacttaa gttattaaga caaatgaata
    21121 aacaactacg ctttcctcga aacaaaaaaa taaagcacaa atgcgcattt acgcactcat
    21181 gctttattcg tacaattaat tcagataacg gcagcgcata ggatgccaat aacacttcct
    21241 aaaatagctc ctcctaaaat gtcagtagga taatgtaaaa gtaaaaaaat cctcgaataa
    21301 gcgatcaaac aagcaaataa aaaggctaat attcctgcaa ctggacttaa ttcgaacaac
    21361 gcagctactg ataaaaaggc tagcgctgta tgtcctgaag gaaaagaaga agtcttcggt
    21421 ttacttgtgt aaaggacaag ccgacgattt ttttcataag gtcttttccg tcttgtcgtt
    21481 tttttgataa aaagcaaacc aacaaaaaaa tagatcaaac tgccgataag aatggctaga
    21541 ccatattctc tagtatcagg tatcaagact aaaacagcag caaggagcaa ccaaataaaa
    21601 ccaccattag agatattact gataaatttc aaaaaattga tttttcgact atccgttttc
    21661 cgttgcttgg cgaacttgtt gataatacta tcatcgattt tagcaatcaa acgcaacatg
    21721 ttattcctcc tttttaagaa ccttttcttg cgtacgatgg ataaaaagca ggacttgaaa
    21781 ataaaacaag aaactgaaac ctaataaagc aaaatagatt ggtcctgcaa accagaatgc
    21841 gacaagaagg atcaacgtgc ctgccaaata gaaaagcgcg cttttgaaat cattaaaaaa
    21901 caagaaaaat gcagtaatcc aagaagcttc tttttgttcc agttgaagca agattttgta
    21961 actaaagaga agcgcgctta atagaagcaa aaacaacgta gtatacatca atgtatagcc
    22021 aattccccca ccccaatagc ggaaaatctg ataatcgatc aatagaagaa gaaaataaac
    22081 acttgctaaa ctatcttgct ttccataaag agagaagttc tcacgaaact ttttccagaa
    22141 tagtgaaccg agctttttct cttcattttt ttgtaatgat aaatcatata cagaactgtt
    22201 cgacttgcag ttcctaatgt gaaaacaggc aagacgcaca ggaaccatag tagctgtaaa
    22261 tggataagac gtggaatcca ttcaatgatt tctaagaaat aatcattggc ttttttacca
    22321 atcgatcgat tcatcatcct tttgaacctc ctgtcaaatc catcccttca atgaaataac
    22381 gttgtgcaaa aaagaacagc aaaatcgtcg ggatcaagac aagtgttgct cctgccatca
    22441 gataattcca ttgtgtcgtt gcttggttct gaaaaacttg caagccaatc tgtaacgtgt
    22501 atttttcttg atcttggata tataacagag gacctaagaa atcattccaa gctgcgttga
    22561 aggaattgat cgcaatggta atcaaggcag gtttcgtcaa tggaagcatc aaatgactcc
    22621 aaatatacaa atgattcgct ccgtcgattt cagcagcttc gatcaattca ttattgatcg
    22681 ataagtaaaa ttgacgcatc agaaatatat tgaacgcatt gccgaagaac gatggaacga
    22741 tcaggggcaa ataagtacct acccagccga tcttggagaa taatacatat tgagggatca
    22801 tcgttacaaa ccccgggatc atcatcgtag aaagaaccaa agcaaacatc agctttttcc
    22861 caggaaaatc aagcttcgca aatccataag caataaaaga atttgaaagg acattaccga
    22921 tcactactag cacagtgatg aataatgtat ttcttgcata gcgccaaaaa ggaaaggcgg
    22981 caatcgtatc cagatagttt tcccatacga ttttttctgg gaaaaaggtc ggaggataac
    23041 gaacgatttc ttccattggt ttcaatgaag taaacaccat ccaaagcaat ggaatgatca
    23101 aaatgattcc tattactgtc atgaggacat aacttagtat ttttttgttt gccgattcac
    23161 actggtcttt ttgaaataat ccatcatttg ccgtcacctc cagaataata gacccactta
    23221 ggtgcaagct tcaaattgac aaacgtcaga accaacacga tcagaaacag gatcattgac
    23281 atagccatcg cataacccat atcaaaagct acgaacgcct tgttccacat atgcagatta
    23341 taaaaaagca aagaatttgc aggtccgcct gttccatttt tcgtcatgac ataagcttct
    23401 tgaaaaattt ggaaagaacc gatcgtagag gtgatcacat cgtaaaaaat aataggagtg
    23461 attaatggca aagtaatact tttgaacttt ccccaaacgc ctgctccatc gatttctgct
    23521 gattcatata aagatctagg aacattttgt aatgtagcta agtaaagtag cattgctcct
    23581 ccagaactcc atattttcat caaaatcaac gcaggtttcg tccattgtgg atcaaacagc
    23641 caagctggtc cagggatatg gaaccagccc agaaatgtgt tgatcaatcc tgcagatgga
    23701 gaaagcagct gcatccataa gatgtagact gctactcctg ataagactgc cggtaaatag
    23761 aaagctgtac ggaatacact gattcctttg attttttgat tgagtactgt ggctaaaaac
    23821 acactgaaaa tcgtcgtcag cggtacattg aagatgacga aataaacggt gttgtataac
    23881 gatttccaaa ataaatcatc gtgaacgaac attcttttga aatttgctag accaatgaaa
    23941 ttcatcttgg atgtcaaatt ataatcggtg aaactaccaa taactgaaaa aagcataggc
    24001 cctagcataa ataccaagaa cccgataata aaaggcgaga taaataaata ggcccatctt
    24061 gcttcttttt tctttcggat agataaacgc ttcaaatttt cttgcctcct ctgtacctag
    24121 acagtcaaaa atggattatt gattttgctt gactaagttt tctactgctt tttgtgcatc
    24181 tgccaatcct tcttttggtg attttgttcc taaaagaatc tggtcaatct gttcattcac
    24241 taatgtcgtg taatccggag catctaaagg aactggtgtg atcaccgtat ttttaaagtt
    24301 ttcgtccgcc atttgataga tcatttttcc tttatccgtt aggttgtcat tattcactaa
    24361 attctcattg gctgtcttgc tcgccatgat atcaaaactt ttttcgccga atttttcttg
    24421 tacttctgga gaagaaagat atttgatgaa ttcataagag gcctcaggat cttttgctcc
    24481 gtatggtact tccaagacga atccgccacc ccacgaccaa tggcctgaac cttcttcttt
    24541 ttctgggagc tgcatcacgc caaaatcaaa atcatccggt gcattttcca tcaaactaga
    24601 gtaataattg atattttggg cacgcatggc tactaatccg gaaataaatg gatcagatac
    24661 accagaccca aattcggctt ctaatttatt gatcgtattt ttcccataat aatcttgcca
    24721 gtcttggatc cactccaagg cttccacttt atttttagta tcaatcttga ctttttcatc
    24781 tttgtcaaac caactagtcc cgttatctgc gtttagcgcc catacatcag cccctaaatt
    24841 ccataatgga taaaaaccga tgcgtgtaaa ttcttttcct tcttttttat ctagtttacg
    24901 tgctgtttct tctaattcat cccaagttgt cggtagatct tgttcactga ttccagcttc
    24961 tttaaatatc tttttattat aaaaaatgac ttgtgtatct gtattgaatg ggatcgcata
    25021 aggatcacct tcatattcta ctgtatccca aagctgcgga taaaaatcct tagaagtgtc
    25081 tttttctaca tatttggaga gattagttgc ttgctgtgct tctgctcttt gggcaacgga
    25141 atttatgtcc tgtacaataa catcgggtgc atttccagct gttattgcag ataaagattt
    25201 agtccagatg tcaccccatg gctgatactt ataatttaca tggatctcgt tttgtgattg
    25261 attgaaatcc tcaatgattt cttcgatagc ttcttttcgt gcaccggatc cccaaaagga
    25321 ccagaaatca atattttttc ttccatcact tgttttttca gaagaatttg ctgcacttga
    25381 actggcatcg cttgcattac ttgatccgca tcctcctaat aaaaacaatg ctgaacttgc
    25441 cagcaaccct gtaaacaata attttttcat attttacctc caaattttaa tgtaaccgat
    25501 gccggtttta atataacgat atcattttgt tctgaaaata acaatttata aggtatgatt
    25561 ctaaaaaaat tattaatgat accaatactt tatataattc gcgagcgctt tctttgaatt
    25621 ttttaatctt tcgtagcaga atgagaatgt attaaaaata gattaggagg aattttgatg
    25681 tttagacacc agaaagaatt gcagtttgaa gcaaaaccag atcgtccgaa tccattaatc
    25741 gcgaaatatc tgcaggagct gatcggcggc caatatggcg agatgagtgt agctatgcag
    25801 tacctgttcc aaggctggag ctgccgagga gaagaaaaat ataaagatat gctgatggac
    25861 attgcaacag aagaaatcgg ccacgtggaa atgttggcga caatggttgc tcgtttatta
    25921 gagggtgcgc ctgttgaaga tcaagaagca gcgatgcaaa aagatccaac aatcggtgcc
    25981 attatctctg gaatgaatcc acagcacgcg atcgtagctg gcttaggacc tcgtcctgct
    26041 gacagcgtgg gcaatccatg gagcggtgcc tatatcattg cgagtggcaa cttgttagca
    26101 gactttagag ctaatttaaa tgccgaatca caaggaagac ttcaagtagc acgtatttac
    26161 gaaatgatcg atgatcgtgg cgtaaaagat atgctgtctg ttctccttgc aagagatagc
    26221 tatcatcaaa atctatggga atgtgcagtg aaagaattag aagaaaaaga aggttcaatc
    26281 ttagtcccaa gtactttccc gcgtgacaaa gaacgaaaag acattgctta tgacttctat
    26341 aacttctcag agggagacca aagtagtgaa ggttcttggg ctagtggcaa agcactggat
    26401 ggcgaaggcg aatatcaatg gttgaaagaa cctaagatcg aaggcgctgc accaaaatta
    26461 gaaccagtaa caccaaaaat gtatgggaca cctccattca agaaataaat atctacccgt
    26521 agaaacaccc gttttttgtt ccacacgctt tgtgagcaaa actgactaaa gtcattaatc
    26581 ataaccaccc ttcaaaaggg tggttttctc tagagctata agctcaaatc accggccagc
    26641 gcctaaagac gctggctttc atattttatg ttcaagccat attgcctttg ccagttttgc
    26701 ccacctctta aaaggtttat gtactactcc ttagccctta aaaggatcgt cttactctcg
    26761 tacactcagc ttatctaacg cactatcatg tttttcttgt tcttgaatgt actttttgat
    26821 ggtcgcttca ttttgtccta cttttgtcac atagtatcct tccacccaaa agtgtcgata
    26881 cccaaattta tattttagat ttgcacaatc tcttgatgat ctcctgaata ttggctcgat
    26941 attgattata aattattttt cttctatact ttggtgtgaa tacaatgtgg tatttacaca
    27001 tctactttgt acgggctaaa ctatttccct ttattgctcc acccgcatag caagtggttt
    27061 tgtatttcaa tcgctcggcg cgctcaatta ggtctaaaga caataaatct aaaaacccgc
    27121 tgcttcactg acagaaacaa cgggttttca gaaaaaaatg aaatgaaaaa gatctcttac
    27181 ggcaatctat tttatttttt tgatttttta taaatggaaa atgcttcaag aagtccaaga
    27241 aataaagccg tataaaaaca agaacctatg aaatctttat ttattagata ttcaacagaa
    27301 atcccgataa cactagccaa caatatattt atcagaagtg taaaataagg atgcttttta
    27361 tagaatagaa ctagtgttgt tttcatagac tctcctttct tcatatttcc aattaatcac
    27421 aaattcacgg cattactaga aaaattaaag gggcgcagaa gtggaaaaat actcttcgac
    27481 tttccctaca aaccaggcca tcttgtgttt aataactata ccaaatctaa ggtacctcta
    27541 aatctaaatt ccatttgtgg cggtgacctc caatttttaa ctcttctatc tcccatccag
    27601 tatacataac cttgatactt cgaccactta tatatcgaac acacaaatac ctatccattg
    27661 caggagtact acctaactta gttgctactt gcatctaaat aacagctgaa aagataggac
    27721 ttactcttag taaagtatct gaactattaa tttttaaact atcattttta tcctcaacta
    27781 catgcacatc tgttgcaact tcactttctg tctcatccag ataaatgatt aaggaatcgg
    27841 gaattgcaat atcttcagct gacgctggaa tagttgagct aaattagtca tcatatcttt
    27901 attataaaat aaccaacagc aaaaaataca atgaaaaaca aacttggaac taatgtatca
    27961 ttaataaatg ttgaaaaatt cagcaactca ttactgtatg attttagcag cccaaccaac
    28021 atatacccta taaaagctaa taagatggtt aggaatcctt tagaattttt actcattcat
    28081 agacacttcc ttaaataact tttattatag aaggtattgt aaataataat attgacaaaa
    28141 agtgactcaa aataaaaaaa accttacatt atactggaat gaactgtatt gatagtgtac
    28201 ctaaaagctc tactgacata agatcgtgta atcggtaaag taattttatt aattatacgg
    28261 gatgtaatca attagagaat aaactaatca taaaaggtac ttttacattc ataattgtcg
    28321 ctttgagatt ctactttggt tcactatctg acccaattgc acattttcga tcattacaag
    28381 tattggtgtc gttagaacaa ctaaataaaa actagcggta gatttttcag aatctatgct
    28441 aatttctgta tggatgttcc agtgatggca aatttctctt ttcattgttt ttcttgtcgc
    28501 atgatatgcc tttttgtgtt attcagttaa actatagaaa ttctttccta gacagatctt
    28561 gatcccaaat tttacgtttt tgagcatcga tatagtttaa aatgacatta ctcatatcta
    28621 aagagtttag attttttagg actaaaactg tcagttgttc tttttcttct ttctgttaaa
    28681 gtattgtcgc gacttttcgt atacgttaag atatgtcgtg aaaaaacaag ttttgtgaca
    28741 caaaaaaacg aatgacttga ggaaatcacc tcaatcattc gtttaaaatg ctatatattc
    28801 tttagtttaa gatacggaaa ttaatgccat cgaccacact agtagaatga acgacattta
    28861 cttggccttt ttttaatttc gttatgttta gcatataaaa atattataga gtttacttac
    28921 aatgcttaaa acatatactg tataatctga tcaattttag ccatgtcttg caatggtaag
    28981 ttttctattt ttttgagttt tcgttcctta aaatcaagag attttaattg tgtaataatt
    29041 acttgtcctt ttatatctag ctcttcaggt aatgagtaac gtgtcggtaa attctttata
    29101 gtggaagtaa ttggacatat gaccgaaaac attgttttac ggttaaattc ataacgagag
    29161 acgactagtc ctggtcgtct tttttgaatt tcttttccta cggagggatc aaaatcaatc
    29221 catacaatat cacctttttt aggaatataa attctttctt ctgacactat aaaatttctc
    29281 tcccttcagg agttaaatct tcccattcgt ctttctcata atactcggcc tcttccccac
    29341 cactaaatgg atcttcaatt tttggaacta acgtaattgt tccatcatca gaatacacaa
    29401 cgatatattc ttggttttct gaaggtttct ttccgttatc agaaggtaaa gttaccacta
    29461 cagaacttcc ttgtaatcta gattttgtaa caagcattat catcactccc ttaataaaag
    29521 tatactatat aacactaagt aacgcaaaat tcccctttgt aaactatcta gaaaattgtt
    29581 ttgtttaaag tatatcttat tggatataaa tagaaataaa ttgtagccag ttcatttttt
    29641 ctttaactaa aaaaataatc caagaaacat tgaacctatg gatccaaaag ctacccaaat
    29701 actatagcta accccccgct accttcaatt ttgcagataa aacgctttgg gagaaaattt
    29761 caaagacaac aggccatcct atcatgcccg attataaaca aaaagctggc tattcctttt
    29821 gctctgacaa atttcaattg ggaaagttca cgaaactagc aggtgaagaa gtttcaactg
    29881 ttcggatcaa agaatgcccg cttcaaatag aaacattggt tcaacgaatt tcaactagac
    29941 aagactttgc tattgtagaa cgtgagatcg tagctatttt tgtagaagaa gggattttac
    30001 atgatccaac tcatatcaat gtggataaat ggcaacctct tatctataaa ttcagagaat
    30061 atacaactgc caaccagtcg ttaactttga attttagatt ccaagaattc aaagagtcat
    30121 aatgataaac atcgttaggt ataaataaaa aagactcgca gcacgagtaa aaaacaaata
    30181 gaagctatac attagaaagg tcgactgtga ccctatagat gcagtatatg cagcttatga
    30241 gcatttcttc tgttctcttt gtttttggga tctgtgctga agtctttttc gttttttgcc
    30301 gctctttctt tgaaaaatcg attatgttat accttctcca ttactaattc aggcatctgc
    30361 tgagtaatga tttttcgcgc aaaagaaata tctgttaccc aaggttcgat ttgttcttca
    30421 gtcaagacaa gcggcattcg gtcatggatt ggtgcgactg tgcggttagg ttcagtagtc
    30481 attatgattg attctgccat tttttcatcc tgatggctac gataaaaacc gccaatatac
    30541 actatttttt ccttatttga aaacataaat ttgtcttttt cttcattcca ctcattggga
    30601 aaacacaacg attttgcaaa aatggttgat gaaaggtctt tttttcttct actgtctctg
    30661 cacgggcatt aatcatcaat tgtccttttt taaatccggt aaatccccac ttcatgattt
    30721 ccggagtgag gatgttttct tcctcttttc ttaacgtcac aacaagattt gacggaaaaa
    30781 cttctccagc tgctaattct gctgcctgct tcggttgttt cgtctgggct tctcgataat
    30841 aattctgaag gagctctgac tctagatcaa aaaaataacg accacacatc ctaatccctc
    30901 ccaatctttt tttctatcat acactgcaac aagaagttcg caccaacaaa acgctttcaa
    30961 tttttgtaga actaaaaaag ttgtgacaag tgacaaactc tccagtgaat ggt
//
LOCUS       DBNICI010000012        30772 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.012, whole genome shotgun sequence.
ACCESSION   DBNICI010000012 DBNICI010000000
VERSION     DBNICI010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 30772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.012"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(392..2467)
                     /gene="glyS"
                     /locus_tag="AC1ZCC_000475"
     CDS             complement(392..2467)
                     /gene="glyS"
                     /locus_tag="AC1ZCC_000475"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HIQ1628754.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2469..3386)
                     /gene="glyQ"
                     /locus_tag="AC1ZCC_000476"
     CDS             complement(2469..3386)
                     /gene="glyQ"
                     /locus_tag="AC1ZCC_000476"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HIQ1628755.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3770..4582)
                     /gene="recO"
                     /locus_tag="AC1ZCC_000477"
     CDS             complement(3770..4582)
                     /gene="recO"
                     /locus_tag="AC1ZCC_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIQ1628756.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4725..5624)
                     /gene="era"
                     /locus_tag="AC1ZCC_000478"
     CDS             complement(4725..5624)
                     /gene="era"
                     /locus_tag="AC1ZCC_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIQ1628757.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5639..6034)
                     /locus_tag="AC1ZCC_000479"
     CDS             complement(5639..6034)
                     /locus_tag="AC1ZCC_000479"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HIQ1628758.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(6018..6494)
                     /gene="ybeY"
                     /locus_tag="AC1ZCC_000480"
     CDS             complement(6018..6494)
                     /gene="ybeY"
                     /locus_tag="AC1ZCC_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIQ1628759.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6511..8703)
                     /locus_tag="AC1ZCC_000481"
     CDS             complement(6511..8703)
                     /locus_tag="AC1ZCC_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family phosphohydrolase"
                     /protein_id="HIQ1628760.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8727..9698)
                     /locus_tag="AC1ZCC_000482"
     CDS             complement(8727..9698)
                     /locus_tag="AC1ZCC_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIQ1628761.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10404..11783
                     /gene="brnQ"
                     /locus_tag="AC1ZCC_000483"
     CDS             10404..11783
                     /gene="brnQ"
                     /locus_tag="AC1ZCC_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HIQ1628762.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11939..12385)
                     /locus_tag="AC1ZCC_000484"
     CDS             complement(11939..12385)
                     /locus_tag="AC1ZCC_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HIQ1628763.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12412..12588)
                     /gene="rpsU"
                     /locus_tag="AC1ZCC_000485"
     CDS             complement(12412..12588)
                     /gene="rpsU"
                     /locus_tag="AC1ZCC_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIQ1628764.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12750..13178)
                     /locus_tag="AC1ZCC_000486"
     CDS             complement(12750..13178)
                     /locus_tag="AC1ZCC_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIQ1628765.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13270..14130
                     /locus_tag="AC1ZCC_000487"
     CDS             13270..14130
                     /locus_tag="AC1ZCC_000487"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HIQ1628766.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(14214..14564)
                     /locus_tag="AC1ZCC_000488"
     CDS             complement(14214..14564)
                     /locus_tag="AC1ZCC_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIQ1628767.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAIIQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(14580..16307)
                     /locus_tag="AC1ZCC_000489"
     CDS             complement(14580..16307)
                     /locus_tag="AC1ZCC_000489"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HIQ1628768.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            complement(16520..17446)
                     /gene="trxB"
                     /locus_tag="AC1ZCC_000490"
     CDS             complement(16520..17446)
                     /gene="trxB"
                     /locus_tag="AC1ZCC_000490"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIQ1628769.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17591..19024)
                     /locus_tag="AC1ZCC_000491"
     CDS             complement(17591..19024)
                     /locus_tag="AC1ZCC_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HIQ1628770.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(19024..20427)
                     /locus_tag="AC1ZCC_000492"
     CDS             complement(19024..20427)
                     /locus_tag="AC1ZCC_000492"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HIQ1628771.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            20657..21559
                     /locus_tag="AC1ZCC_000493"
     CDS             20657..21559
                     /locus_tag="AC1ZCC_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIQ1628772.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(21600..23486)
                     /locus_tag="AC1ZCC_000494"
     CDS             complement(21600..23486)
                     /locus_tag="AC1ZCC_000494"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HIQ1628773.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(23583..24434)
                     /gene="licT"
                     /locus_tag="AC1ZCC_000495"
     CDS             complement(23583..24434)
                     /gene="licT"
                     /locus_tag="AC1ZCC_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HIQ1628774.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            complement(24892..26676)
                     /locus_tag="AC1ZCC_000496"
     CDS             complement(24892..26676)
                     /locus_tag="AC1ZCC_000496"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1628775.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(26676..28427)
                     /locus_tag="AC1ZCC_000497"
     CDS             complement(26676..28427)
                     /locus_tag="AC1ZCC_000497"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1628776.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            complement(28569..28793)
                     /locus_tag="AC1ZCC_000498"
     CDS             complement(28569..28793)
                     /locus_tag="AC1ZCC_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HIQ1628777.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            29044..30630
                     /locus_tag="AC1ZCC_000499"
     CDS             29044..30630
                     /locus_tag="AC1ZCC_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HIQ1628778.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT         8592 a         6604 c         5477 g        10099 t
ORIGIN      
        1 gattgacgtc ataaaaagta gctttgaatt cataatcgtt tatataacga ttaatagaaa
       61 acaatatagt atttagtgtt catacaatga ttctaagatc attttgaatt aatcagtctt
      121 atagttgatt tctaaattgt catatattat taaaaatttt tgccatttaa aaagaaaagc
      181 gacagtttac caataaattt caactgaatt atctaaaagt agatcagtta gtttagataa
      241 ttcagttgaa attgaccgac tcaaattact tgtataatac ctaggaaaaa attaactagc
      301 accacgggta tagttattta tatttataac aaataaacca gagaaatagc ctattttgtg
      361 cctattctct ggtttattta catattagaa attattttgt gatgatttga tctaaacttg
      421 ctagcgacaa agccatggca gcaatttgac ttaattgcgt taatcggttc ttacgtactg
      481 ctggttcttc tgccatgacc atcgtctgat caaaataatt ttcgatcagt gggcgaaggc
      541 ttacaagctg atcataattt tcgttgatcg tttggtcagc aaagcttttt ttcattgcct
      601 ctactgcttt gtgcagttct ttctccgctt cattttcgaa cagttcaggg tctacttctg
      661 attgtttttc aagctccgct tttttcgcta agttcacgac acgtgtcaaa gcttccatcg
      721 atggtttgaa gtcttcatct gcaagatgct gcttgaacaa ttgtgctgct gcaaatactt
      781 ttgtcagatc tttttgttct gcattcaaaa cagcttcgat gacatcatga cgaatattct
      841 tcgttgtcaa taattggcgc agacggcctt tgataaagtc gatcacctgt tgttgccctt
      901 ctgataaacc aatgccgaaa cgatccgtat ctttactgat aacatcacta atcgtttctt
      961 gcagaacaga caaaggaaag gcccattttt tcgattcgat gatccgaacg acaccgtatg
     1021 cttggcgacg taaggcataa ggatcgtttg acccgcttgg aatcatccca actgcaaaga
     1081 aagaaaaaat gctatctaat ttatctgcaa tcgctaaaac agaacctact gtagactgag
     1141 gtagctctcc ttcactggat ataggcatgt aatgctcacg aatcgctgtt gctacagcag
     1201 gtttctcgcc tttcagcaga gcatattttt cgcccatgat cccttgtagt tcaggaaatt
     1261 cccctaccat gttggttact agatcaaatt tatagatttc agcagctcgt tgcagatcca
     1321 tcaattcttc ctcagatagt cctacttgtt cgccaatgat acgggcaatg acttgtaccc
     1381 gctgcatttt ctcatagata ctaccgattt tttcatggaa agtaacagtt tttagttttt
     1441 caacgtattg gtcgattgaa gctttcttgt cttcattata aaagaattcc gcatcttcta
     1501 aacgagccgt caatactttt tgattgcctc gaaccacatt ttccaatttt acccgatcac
     1561 cattacgagc agaaataaaa tgaggaagca gcataccttg atcgttccgt acctcgaaat
     1621 aacgttgatg ttctttcata gaagtgacta gtacttcttc aggaacagaa agatattttt
     1681 cttcgaagtc gccagaaaag acagttggat attcgactaa attgttgact tcttctagta
     1741 aatcttcgtc tagatcaata acccagtgat ttttttcggc taattcattt gcttgtgtaa
     1801 cgatcatttc ttgtcttttt tgcggtgaag caataacgaa ttgttcattc aatttttctt
     1861 catattcttt tgcatgcgca aatgtcacat cttcgcctaa gaaacgatgt cctcgagagg
     1921 ctctgcctgt tttgacatct aagatctcaa acgggattac ttcgtcatct aacaatgcca
     1981 cgatccagtg aatagggcgg atatattcaa aatcgtattc tgcccaatgc atcgttactg
     2041 ggaaagtgag gcttttaatg acatctgcta aacctgtcaa tacttctttc gcttctttgc
     2101 cgtgagtgta tttcgttaca tagacatatt ccacgccttt taattctcta aaagtgatag
     2161 catctgtcgt cagaccttgt ccgcggacaa atccttcagc tgctttcgtc cagtttccat
     2221 ctgcgtccaa agcaattttt ttggctggtc ctttgacttc ttcttcactg tctgcttgac
     2281 gttctggaat gtttgttact ttgattgcta agcggcgcgg tgtagaaaac gtttcgatcg
     2341 tatcataagt caattggttt tcatctaaaa attttgctgt tttttcttct aattgtttca
     2401 tgcttggtgt gacaacatga gcaggcattt cttccagccc gatttcaagt aataagtctt
     2461 ttgccatctt attcagcctc cttcatgttt tctggatttg ctgtgttttt attcaataaa
     2521 gggaatccta atttttcgcg ttctgccaca aagattttgg ctacagcacg agccatgttg
     2581 cggatacggg aaagataacc tgctcgttct gttacggata ctgcaccgcg agcatctaat
     2641 aaattgaatg tatggctgca tttcaaaata taatcatacg ctggatgaac aagattttca
     2701 tcgatacagc gtttagcttc tttttcaaaa cggtcaaagt tgcttagtag caagtcttgg
     2761 tcacttactt caaaagaata tttggaatgt tcatattctg gttgtttgaa gatctcgcca
     2821 tatttcacac catcagccca ttccagatca tagacacttt caacttcttg aatatatgaa
     2881 gcaagacgtt ctagtccata agtgatctca gaagtaactg gtttacaagg tagtcctccg
     2941 acttgctgga agtacgtgaa ttgtgtgatc tccatcccat caagccatac ttcccaaccc
     3001 acaccggcac accccatcga tgggttctcc cagttgtctt caacaaaacg aatatcgtgt
     3061 tctaatggat caattcctaa aagacgcaag ctttctaaat aaagctcttg gatattttca
     3121 ggtgaaggtt tcattacgac ttgaaattga tgatgctgat acaaacggtt tgggttttct
     3181 ccataacgcc cgtcagctgg tcggcgggat ggctctacat aagcagcatt ccatggttcc
     3241 ggtccgattg cccgtaagaa tgtatatggg ctcatcgtac ctgctccttt ttctgtgtca
     3301 taagcttgca tcagcatgca gccgtttgat gaccaaaatt tttgtaatgt taaaatcata
     3361 tcttggacgg tcaatttttt actcatctgt tttgcccctt tcaattttta agcgttgctg
     3421 tcaataagaa tacaggaaag aaaacgacaa aaagtcccta tgtctgatta cagacatagg
     3481 gacgaataat ttcgcggttc caccctactt ccgaataaag attcggcagc ttcatttatc
     3541 gtactcgcaa gtgcctcttc aaaaatcgtt tgggtccggc ttccactctc ccgaactcgc
     3601 taaaccacgt gttttttact gatcttggtc aatgtacgta ttcattttgc agttttcttg
     3661 tttatataat agatcctctc agtgaacgat ctatttaatc tacggttaat aatagacgga
     3721 agtccctgta tttgtcaaat agttttgatt tttttgttgt atctttcatt tattcttctt
     3781 tttcaggtat ctctttttca ggcatatttt tctctggcgt ctttaatacg tgtgcccaag
     3841 acttcatttg atcaatgaac tttttgcttt ttagatgaat accgacatat tcatcataca
     3901 actggtcaat gaggtcacgg attgcctgct tcgtttcttc tttcaagtgg attccactga
     3961 tcttatcata agaaatagcc gaaaacattc gaacgaaata aacagcgcga ggatctgcat
     4021 gataacggtg ttcgtccatt ggccagtgtt tctcgcatat cacaccgcca gatttggaag
     4081 aatagtcaaa ttttcctttt gtttccccgc atacgacaca gtgcgtccaa attggcgcga
     4141 ttccgaagcg ttgcagtatt tgaacttcaa agatattcgt gatgatttct gcatcatctc
     4201 cttgatccat ccgctgcaga gcttgttgcg taaattggaa taaatgcggg tcatattgat
     4261 gatcttcgat tgctgcatct actagattta gcaaataggt accataaccg ctcaaaaaga
     4321 tatcttcttg gatgttccga aacgcatgga cgtctttgac actgcttaaa aacgacaaac
     4381 cttcacttcg aaaattaccg atatagactg cttcggtaaa ggggagcaaa gccggagcta
     4441 atggctgatt ttgccgatgg attcctttga tataaaacat cattttgcca gaagattccg
     4501 taaagatttt gactaattta tctttttctt tgtaattacg gctagataaa atcaagcctt
     4561 tcgattctgc aagctggctc atattctccc tcctaaaaaa taaatctgga aaagcagatt
     4621 ctgccgatcc agattattta tgtttcgatc ttccgccgtt tttctcaacg tgcggatttt
     4681 tatccagttt atttctatac ttaacctata agaagttcag aatatcagta ttcgtctttt
     4741 cggtagccaa agtcttgcag atagactttt ttgtctcgcc agtctttttg gactttgacc
     4801 caaagttcta gaaatacttt gtctcctaaa agattttcga tatcctttct tgctttcgtt
     4861 cctacttgct tcagcatttt tccgcctttg ccgataatga ttcctttttg gctatctctt
     4921 tcgacgatga tcgtggcctg gatatggatt ttatcattct cgtttcgctt catcgaatca
     4981 acaacgactg cgacagaatg aggcacttcg tctcgtgtta aaaacaagac tttctcacgg
     5041 atcaattcag aaacgataaa gtattcagga tgatcagtca cttgatcatc tggaaaatat
     5101 tgtgggccct ctggcatttc actgactaat acatccatta agcgttcgac attgtttcct
     5161 tctgtagcag agatcggaat cacttgtgca aaaggcatct gcgtcgaata atcttcgatg
     5221 attcccagca actcgtctgg atggatctta tcgattttat tgataatcag ataaactgga
     5281 gaatgcacgt tttttagacg ttcaataata aaatcgtctc cccggccgcg tttttgatca
     5341 gcactgatca taaataagac tgcatccact tctttcaacg cactgtaagc tgcttctacc
     5401 ataaaatctc ctagacgatg ctttggttta tggattcctg gtgtatcgat aaaaatcagc
     5461 tgagcttctg gtgtagtata tactccttgg attttatttc tagttgtctg tgccttgtca
     5521 ctcataatgg caattttttg acccacgatt cggttcaata atgtggattt tcctacattc
     5581 gggcggccga cgattgcgac aaaccctgat ttatgttctg gcatatacgt tctccttttt
     5641 aattaaacaa caaatgatat attttaggga caaataaaat caacccaatg atgacagcaa
     5701 aacaagcggt cactaaaact gctccagctg ccatatcttt gatttttttc ccaatcggat
     5761 gaaaatgaaa atccgtaaac atgtccacta cattttcaaa aacagtattg atgatctcta
     5821 ctatccaaac gagaaaaaca gctaaaagca gccataacca ctctatccga tcaagctgga
     5881 aaatcacgcc cataaataaa gcaaggatac cgaacgcaac atgtttcctc atattgcgtt
     5941 cttcctcaaa aacggttttg actccttgga cagcaaattc cacagacatc aggaaatgtt
     6001 tgtttttctc tacgttttta tcttttgagt ccataggcat ccaagatctc tttctgtaaa
     6061 ccaaacattt ctttctcatc ttctggtgtc atatggtcat acccgttgat atgcaagaat
     6121 ccatgtaccg ctaaaaagcc aagttcccga tcatatgaat ggccgtaagc ttctgcttgt
     6181 tcagcagcac gttcaataga aatcatgatg tctcctagta cacgaggcaa aggattctct
     6241 ccatcttcaa aaatcactgg gatttcgtct tcgccttctt cttccagagc aaaactgatg
     6301 acgtctgtcg gtgcatcttt tccacggtaa tcacgattga ttacttggat tgccgcattg
     6361 tccatgaaag taacagacat ttctgtttcc tctggcagct ttaaataaga tgctgcaaat
     6421 tgcagcaagt catcgatttc tttcattttc tcttctgaga cttggtttgt ttcatccata
     6481 aaggtaatat ccattataca tcgcttcttt ctattcttct tgttcttcct ttatcttctc
     6541 aacttctgtt ttcattttag gatatttgat ccgagaatgg aaggtgctac ttaaaccgct
     6601 gatcaatgat ttttccactt ttcggatttc tttcaaggtc agtccactat cgtctaattg
     6661 accatcggaa atacgctctt cgataagatt atggacaaaa ctttcgattt tttcgctcgt
     6721 cggatgatcc attgcccgta ctgcagcctc acaactgtca gcaatattga cgatccctgc
     6781 ttcacgactt tgcggttttg gtccaggata acggaattgc tcttctgtta cttccggatt
     6841 gcgttctttc gctttgatgt agaaataacg cattagcgtg gttccatgat gctgtttgca
     6901 aatatcgatc accatttgcg gcatattata atcttccaat attttggctc catctgtcac
     6961 atgtccaaag ataatatgtt tactgtcttc tggcaataag aaattgtgcg ggttttccgc
     7021 tcctgaaggc aagttctcaa cgaaaaagct agcatgtttg atcttgccga tatcatgata
     7081 atagcaagct actcgagtta gcaaagaacg tccgccgatt tctgctacag cgttagcact
     7141 tagattagct accatcatac tatgatgata agtacctggc gcttcttcca gcaactgttt
     7201 caataaagga tgatttggat tgctgagttc atttaataca attacactgt catcattgac
     7261 catcaattca atatatgggt gtagaccgat cgtcagcaag aatgagaaga gtgcaccagt
     7321 gaacccgcag acaagcatta accaagtcgt tgcatcacca aagctcattc cttgatagac
     7381 gatcaaaatg acatccatca tcacaggaaa aataatgacc cacatggcag cagataatcc
     7441 ttgttctgag atccgtttgc gtctgacaat cgttgccatg agcccagaga aaacataaga
     7501 catcaagata atcgttagaa agttcgtgcc aatcgaatca taaaaaatga aaagtgcaga
     7561 tactgcttgg aaaataccag ccatgatccc tgctcttcgg ttcaagaaaa agcttaaaac
     7621 aagtggtgca aaagcagcgg gataaaacag cgggatataa gtagcttgtt ccgtttggaa
     7681 taattggaaa aatttcatca gcactatgct gactgacata gctgtcacat agaaaagcac
     7741 gtattttgtt ctttctcctg cattttggaa ttgaagtgaa ttataaatca gtacgccgat
     7801 ttgaagcaaa acagctaaaa ccatggcaat gagtgggaaa atcgaagtcg tttgattcgt
     7861 caatcctagt aattccaatt ttttcatcgc tgaagcatca atttgatttc cttcgcgaac
     7921 aatgatttct ccttggaaga tcatcactgg ttgtacagct tctcgtgcag attgttttaa
     7981 ttcttctgtc tttttctcat tcaatacatc attgactaca atgccttgat ccactaagta
     8041 gcgaatcatt tgttgctgct ctgttgtgac attcaaatac tgaatctgat cttctgcttc
     8101 ttgtttaaaa gcttccaatt cgctttcacg aacttgttta ctcatctgtt cgtcaatgag
     8161 cttcaaactt tcatccctaa ccgtgtgaat ttccgtttca gtcatttcaa atatcgtcga
     8221 ataaaaattg ttcggcagtt tttggtaaaa cgcaacgttt tctgcattca cgttttcaaa
     8281 atttttcttc agtgtagcaa tccgctcttc tgtagtaggt ttcgggatat tttcatcttc
     8341 cttcgcctta tctaccttgt cattataaga tttgttgatg gtatcattcg tcttatcgat
     8401 caattcgaat aattgcttga tccgattgtt ctggtcatct gccagatctt tttgataagt
     8461 atattctgga gtcactgctt ctgctgccag ttttcttctt tgttcggttt cttccgtgtt
     8521 ctcgatcgtt ttattcgcac ggatactctc ttctgctact tggccttctc tgaaatcatt
     8581 ggccttttgt ttgacactac taaatgtaac gccgcagaca atgataaaaa acaagaacag
     8641 aaggaaaggc aatgctgcct ttccgagttt ttcttgtagt ttctgcagtg ttttattcaa
     8701 catgtgctta tcctcctaag caaagcttat tcttgatgaa gatccgcctt ttcataagct
     8761 ttgatgattt cggcaactac agggtgccga acaacgtcac ttgcttcaaa attaacaaaa
     8821 tcgatttttt tgatttgctt tagtgttcgt tctgcatgaa caagaccact tgtcgtccct
     8881 cgaggcaagt ctatctgact tgtatcccca ttgacaatca tttttgattg ataaccgaga
     8941 cgggtcaaga acattttcat ctgagcgact gtcgtgtttt gtgcttcgtc caagataacg
     9001 aatgcatcat ccaatgtccg cccccgcatg taagctaatg gtgcgatttc gattacgcct
     9061 cgttccatca atcggttcgt atgatccaat ccaaaaattt ggtataaagc atcataaaca
     9121 ggccgcagat aaggatccac tttttctttc agatccccgg gtagaaagcc taggttttcc
     9181 cctgcctcta cagcgggccg cgtcaaaatg attttttgca cttcgccttt ttttagggca
     9241 gcaattgcta atactacggc aagaaacgtt ttccctgttc cggctggccc tacaccaaat
     9301 acgatgtcat gtttggctac cgattcaacg tatttctttt gaccgctgtt ttttacccgt
     9361 ataggttttc cattacggtc tttgacgatc tcttcctcat acatctcaac aaaataatct
     9421 aatgtacctt tttgccccat tttcaatgcc gtcacgacat ctggtgtact tacgtggatt
     9481 cctcgtttga tcaattcctg taatgtccga atgactgagg cagccaagga aatctgggat
     9541 tgttcaccaa tgatctgaat gatttctcca cgtgtatgga tggttgtttc cgttgtttcc
     9601 tcgatcagtt ttatatgctt gtcgtgtgca cctaaaagca tgctgacatc atcagcagct
     9661 gtcaatctaa tctctaaaga actagactct tccgtcaaaa atagacatcc cctttttttc
     9721 gattcattct ttcctatttt accatactca gaagaaacta tatagcaaat aagaaaaggt
     9781 tgtaaaagag tcgtttaacc atacgtacca agaaacgtta aaaaatagct gtttccattc
     9841 gctaaaggtc tcgttttgat aaaaagaatc tgagaatgta agttatatga aagtggagaa
     9901 caaaatattc cgtttcattc agaggtattc tctaggtatt tttcttgttt caaaaaaaga
     9961 aaacaaaata gttaaaatat tcttgcattt caaaaaaaac aggtgtatta ttacgtcaac
    10021 aaataaacat acagcaaagc gtcgaggaaa acagtaggct attcaacgat ttattcccag
    10081 tgagtcatgt aagtgagaaa tgacaataaa tcgaatagac gaacatcatt tccgagtcaa
    10141 tgtcgaaaac gtagatcatt gtggctgcct ccattatcag gctagcggat aactttatca
    10201 aaaagcgtcc gtgaatgaag aagctttttt aagcgaaaaa aggtggtacc gttctagaat
    10261 ctagagccct tttcccaagt ggaaaagggc ttttttttat attttaaggt ggtggttttc
    10321 atggaatctg atattagtgg cattactaat tgctgcaacg tttatatcgt caatcaaaat
    10381 gataattatt ggaggaaaaa tttatggaag aaacaaaaac ttattcatgg aaacaactat
    10441 tgatggtcag ttcacttatc ttcgggatgt ttttcgggtc aggtaatcta atctttcctg
    10501 tccacttagg tcaaatggct ggcgcaaact gggtcagtgc tggcgtagga tttgccatct
    10561 caggcgctct tttcccatta ctagctatct tagctgtagt agtgacaaaa agtgatggtc
    10621 tgtatgactt agctaaacct gtcggaaaat ggtatgctgc cttgttctta atccttgttc
    10681 atttgacgat cggaccattt ttcggtacac cacggacagc tgctactgct tttgaaatgg
    10741 ctgctaaacc atttttgcct gaaaaatata attcattagg tatgttttta ttctctgctc
    10801 ttttcttttt acttgcttat ttccttactg tccatcccac acggttggtc aaatacgtcg
    10861 gaaagtatct aaactccttg tttcttgcat tattagcaat catttttgtc gtttctttat
    10921 tccacccaat gggaaatcta aatcaatctg ctgcagatac ttaccaaaca aacgctttaa
    10981 caaatggtgt cctagaaggt tataacacgg ttgatgcagt agccttgctt gccttaagtg
    11041 tcacttttgt ccatgcagtc cgtgaccttg gtttcaaaga taaaaaagtc acagaattaa
    11101 cagcaaaagc tggtagcctg agcgttgcgc tagaagtcat gatctatttt ggtcttgttc
    11161 ttctcggtgc gatgagtttg aataagatgt ctttatcaga aaatggcgga actgcacttt
    11221 ctcagatcac taatttttat ttaggtaact tcggcgctag cttcttagga gtgatggtca
    11281 cattaggtgt cttcactacc gctatgggac tcgttgtttc ttttgcacaa gatttccata
    11341 aactgttccc taaagtcagt tatatgacat ggctgcggtt aacgactttc gtctcttttg
    11401 tcgttgcaaa tgctggtttg gacaatatca tccaatggtc tttaccagta ttgatgcttt
    11461 tgtatccatt gtcgcttgcg ttgattttgc tttccttaac agcaaaattt ttccaaaaga
    11521 caccatttgt ctatcaagta acgatgttgt ttgctgctgt tcccgccgta ttggatatgc
    11581 tggcaaattc gcctgcttta gtgagtcaac aaagagttgt agcaagcatg ctcgaatttt
    11641 atcatcatca cgttcctttt gctgcattgg gacttggctg gatggtccca acgttacttg
    11701 ggtatgctgg cagcttactc ttctattacg cctatcgcct atcagggtat aaacaagaag
    11761 caaatgaact accagaagaa taaccagatc aaacacaatt taacataaga aatattaaac
    11821 aggaaaaaaa cagctagatg aaagaatcct attctttcgt ctagctattt tttagatctc
    11881 ttttttacga gcacaaaaaa actgcggaca atgattgtcc gcagtttaaa tgataaaatt
    11941 aagattgtaa tagttctttc acaatggcat ttacttggtt tccatctgct tttcctttga
    12001 ctttaggcat gatcgcaccc atgactttgc caaattcttt cattgatgaa gcaccagttt
    12061 gcgtgattgc ttcttgaaca agttgtcgaa tttcttcgtc ggaaagttgt tccggcatgt
    12121 atttttcgac tatcacaatt tcacttttta ctttttcagc taaatcgtca cgtcctgctt
    12181 cttcaaactc atgcaaagaa tcacgtcgct gtttcatttc gcgagacaac accgttaatt
    12241 cttcttcttc tgtcaagtcg tgaccttctt taatctgctc gttttgtata gatgacttga
    12301 tcattcggat gacttgtaaa gattccttat ctttcgcttt catcgctgtt ttcatgtcat
    12361 cgtttaatct acttagtagt gacacactac caactccaat tcatcacaga actagaattt
    12421 tttgcgtttt ctagctgctt cagatttttt cttgcgtctt acacttggtt tttcgtagaa
    12481 ttcacgttta cgagattctt gtaaagtacc cgcttttgac acggaacgtt tgaagcgacg
    12541 aagagcatcg tcaagtgatt cgtttttgcg aacgactgtt tttgacatgt taattccctc
    12601 cctccgagct taaaaagtaa ccgtaattgt tattgaaata tatctcaaaa aacaagactg
    12661 tcctgtaaat attatatcta atgctgtttt gaccgtcaag gaaatcttgc ttttttttaa
    12721 aattatcaaa tgttatttta tttaaatcct tattgacaat tttcacatct tccgaaaatc
    12781 tcgaatctat gcccttcgat cgtacatcca ccgagctgtt cttcgaaata attcattgga
    12841 cacatatgga tttctttcgt tgttccgcac accgtacaga taaagtgatg gtgatggccg
    12901 atttcccctt gacagcaatg aaaacgaaac ttcatctctc catttaaatc ggtttcttcc
    12961 agtaaatcga tttctgcaaa atcatggaga tttcgataaa tcgtatcgta acttactcct
    13021 ttgaattcct gattcatata gtcaaaaact tctttagcgg atacgtagcg attgcgcttc
    13081 actaaataag ttaacaatgc ttctcttttt ttagtatatt taaaaccgtt atttttcaat
    13141 gtttctagtg attgttcgat caacgtctgt tttttcatga cttattcctc caaatcaaaa
    13201 tgattacgaa gtacacattc tagagtatgg tataataaac ataaaaaaaa tgcaagtgag
    13261 gttttatcca tgtcttccga ttctacagaa atcgttactt tttttgtcat ctccgactct
    13321 gctggagaaa ctgcgacaaa actagcgcaa gccactatgg cccaatatcc tagcgttgag
    13381 ttcaatttat tccgcagaac atttgtgaca gatgagaaaa cattgatgca ggcactgcaa
    13441 gatgcactag aagaaaaagc tcttgtcctg cacacattga tcaatcaaga attgatcgag
    13501 atgacgcgtt ctttttgtga agaacatcaa ttatttagtt ttgatgtgat gactccgccg
    13561 atatcagaaa tcgagcgctt aactggtatc aagcccattc ggcaacctgg cgcccttcat
    13621 ttattgaatg agaactattt caaacggatc aaagcaatgg aatttgctgt aaaatacgat
    13681 gacgggaaag atcctcgtgg gtttctagaa gcagatgtcg ttttgctagg cgtatcccga
    13741 acttccaaaa cgcctttgag cttgttttta gctaacaaaa atttaaaagt tgccaatcta
    13801 cctttgatcc cacaagcaca tattcccaaa cagctttggg agatcgatcc taaaaaaatc
    13861 gtgggactga cgaataaccc agatatttta aacaatatcc gtaaagaacg gatgcgctct
    13921 tacggattaa atcctgatac tgcttattcc gatatcgaaa aaatacgagc tgaactcgat
    13981 tttgcgaatg atctttatga aaaattgggc tgcgtagtaa tcaatgtcgc ttctttatct
    14041 attgaagaaa cagcttcgct tattttgaat gctttagacc tagaagacca tagttattat
    14101 ggaactgaaa ccaccgaaga aacagactaa caatttttct ttaaaaaaac tggactgtgt
    14161 cagcgaagaa gattcgttga cacagtccag tttgtctcta tttgcaccta tcgttattga
    14221 tgttccacat gtttgagtgt ctggttcaaa atatccagta cgtgagaatc atcaagacta
    14281 tacaaaatcg cttttccctc acgatgggac ttgacgacat ggttttctct taataaacgc
    14341 agttgatgcg atacagcaga ttgttccatt tccactgctt ggatgattgc tgtcacattt
    14401 tgttccccgt ctttcagata cagcagaatt ttgagacgag tggggtcact caacattttg
    14461 aatagttggc tgactttctc tacttttgtt ttttgcgtgt ctggcaatcc attcacctcc
    14521 aacttgaaaa aagactgact tctaaagttt accacttttt ccatcagtct caagtttttt
    14581 tattcttctg tcatcttttg gataatcgtt tcataagcgg ctactttttc agctgcgtct
    14641 tctgaagagt ttccttttac accgatataa aatttgattt tcggctcagt tcctgaagga
    14701 cggatagcga tccagccatt ttcttccagc gtatacttca atacgtcaga tggcggtgtc
    14761 gttagttttt ctacgtttcc ttcacggtct gttctcgtca actctttgaa gtcttctgtt
    14821 tggatgactt ttgctccggc aaattctgtt ggtgcttctt cacggaattt ttccatcaaa
    14881 gcagagattt tcgcagaacc ttcttgtccg ctcattgtca cagaaatcgt tttttctgcg
    14941 tagtagccat aggtttcaaa aatcgtttgc aagccatcat acaacgtttt gccttgtttt
    15001 ttatagtaag ctgctacttc tgctaataag agcaatgctt ggatcgcgtc tttgtcgcga
    15061 acgaaaggtt taactaaata accgtaactt tcttcgaatc cgaacataaa agtatgggaa
    15121 tgatcttctt caaattgttg aattttttca gcaatgaatt tgaatcctgt caatacattg
    15181 aacatggtag ccccatagtg ttccgcaatt gctgtaggta attcgctaga aacgatcgat
    15241 ttcaataccg ctgcgttttc aggtaaagtg cctcgcgatt ggtgtgcttc caaaatatat
    15301 tgagcaaaca gtgctccgat ttggtttccg gtcaatactt cataggagcc atttggcaaa
    15361 cgaacggctg cacctaaacg gtctgcatca ggatctgttg caatcagcaa atcagcgcct
    15421 tctttctcgc caagtcgtat cgcatactca aaagcagagt gttcttctgg atttggtgat
    15481 ttgactgtag tgaaatctgg gtctgcgaca gcttgttctg gaactagtac gaattgttcg
    15541 aatccagctt gtttcaacgc acgttcgccc aacatttttc ctgttccgtg aagtggtgtg
    15601 tacacaagtt tcaaatcttt ccccattgtt tccaccaatt catgatcaat cgttactgct
    15661 ttgatttctt ctagataagc ggcatccact tcttctccga tgatcgtgat caatccgcta
    15721 tgttcagctt cttcgtcaga taatacttca acttttaacg gattgctgac ttcacggaca
    15781 tatttcgtca atgcatctgc gtctgcagga ggcatttgtc ccccatcttc tccgtagact
    15841 ttatatccgt tgtaagctgc tggattgtgg gatgcggtta ccatgattcc cgcaaacgtc
    15901 ttcaaatgac ggactgcaaa agaaagctct ggtgttggac gtagactttc gaatacataa
    15961 gatgggatat tgtgatgtgc taatgttttg gctgcttcca tggcaaattc tggtgattga
    16021 tggcgtgagt cataagcgat agcgacacct cggcgttttg tttcttcgcc ttgtgtatcc
    16081 atgaaacgtg caagtccttc tgttgcttgt cttaccgtat agatgttcat tcgattgatc
    16141 ccaggcccta agattccccg catgcctgca gtaccgaatt ccaacggtgc ataaaaagca
    16201 tcttcaagtt tttcaggatc ttcacccaga tcatttaatt gtttttttaa gttttcttct
    16261 aaattttctt catgtaccca ttgttcatac acttgttccc aagacatgaa aataacacct
    16321 ctttctgaaa tatggttctt aataagtata gcattcactt attagatatg aaagtatgag
    16381 gaccaaaaaa cgcttaaatc aagaaaggat tcttttatca agcacggttg caagtattcg
    16441 cataaatcgg attcagtaga tttcacgcga aaaaaaagag taaaagaaca attaaaccgt
    16501 tcttccactc tttttaaact tatgcagttg tttcttctaa agattccaag tatttataca
    16561 cttgttgtcc agcgatccca ccatcaccaa cagcagtagt gatttgtcgc aaatcttttt
    16621 cacgaacatc tccgatggca aaaacgcctg gtatattcgt cttcatttcg ttgtctgttg
    16681 gaatccagcc agcttcgttc gtcaaccctg cttctctaaa tggtgcagtt aatggatcca
    16741 gtccaacata gatgaatact ccgtctgctg ccaaatcaaa ctcattgccg gttttgacat
    16801 tacgtgcttt cacacctgtc actaccattt cattcccaac gatttcgtca acgactgtat
    16861 cccacataaa ggaaattttt tcattcgcaa atgcacggtc ttggatgatc ttttgtgcac
    16921 gtaattcatc tctacggtgg acgatgacta cttcagaagc aaattgtgtc aaatagatgg
    16981 cttcttctac tgctgagtct cctcctccga tcactactag acgtttgtta cggaaaaacg
    17041 caccatcaca aactgcacag taagaaacgc cacgtccagc aaattcttcc tcgccaggta
    17101 cgcctaattt acggtggata cagcctgttg cgataatgac tgtttttcct tcgtaagttt
    17161 tatcttcaca aataacttct ttgtggctgc cgtgatcctt gatatcttgc acgattccat
    17221 aagcattttc tgtcccaaat cttgaaacac cttcatacat cttataagct agatcaggac
    17281 ccattattga atcgaaacca ggatagtttt cgatttctgc cgtattgttc atttggccgc
    17341 caggggcgcc ttgttcgatc atcaatacgg ataagtttga acgtgaagca tataaagcag
    17401 cagtcatccc agcaggacct gcgccaataa cgattacatc atacatatgt agattctcct
    17461 ttacttaatc aagtaagcat actttacagc caatgcgaaa aaaagtctat tgttctgctt
    17521 gcgtttctcg aatagcttta ttatactcga aaaatccgct gaaaaacaat agacttttat
    17581 aaatcattga tcaatcattc gaaagatttt ctccatttga atcaattact tttttatacc
    17641 agttgaatga tttcttcttc gaacgggcta atgtgccatt tccttcgttg tcacgatcga
    17701 cataaatgaa tccgtacctt ttcttcatct cacctgtacc agctgatact aaatcaatac
    17761 atccccaagt cgtatagcct aatagatcga ctccatctaa ttctactgca tctttcattg
    17821 cctggatatg tgctgccaga tagtcgattc gatagtcatc ttccacataa ccattctcat
    17881 cgggcgtatc gactgcacct aagccgtttt ctacaataaa tagtggtttt tgataacgat
    17941 cataaatatc attcatcgtt attctcaagc ctaatggatc aatttgccaa ccccattcac
    18001 ttgcttccag atatggattt ttgacagaag caaagatatt tcctgcagtc tgttccaaga
    18061 gttcgttgtc agtagtcgaa actcgagaag aataataaga aaaggagata aaatcaacag
    18121 tatgttcttt cagcaatgcg gcatccccag gttccatttt gatctgaatg ccatttcgtt
    18181 ccatttcttt caatgcataa gcaggatatt ctcctcgtga ctggacatca atgaagaaat
    18241 aattttctcg atctgatttt ctagaagccc aaacatcttc aggattgcaa gtgtatggat
    18301 aatatgctcc tgctgccagc atacagccta ctttgttctc cggatctact tcatgagcaa
    18361 tcttcgttgc gatggcactt gcaaccaact catgatgtgc cgcttgatat ttgatttgtt
    18421 cttcattttc tccttcttca aaatacagcc ctgcgcccat gaatggtgca tgaaggatca
    18481 tattgatctc attgaatgtc aaccaatatt tgacaagtcc tttatagcga ttgaagatga
    18541 ctcggcacag attttcataa aaaccaacga gttctctgga acgccacgca ccatattctt
    18601 tgatcaaatg catcggacaa tcaaaatgtg taatcgtcac aagcggctcg atcccgtact
    18661 tttgacattc tttaaagaca tcctcataaa atttcaaacc agattcattt ggctcttttt
    18721 catcgcccat tggaaaaata cgactccaag caatggacat ccgataagtc ttgaacccca
    18781 tttctgcaaa taaagcaata tcttccttat aatgatgata catatcgatc gcaatttttg
    18841 ctggataaaa atgattttca tcaaattcaa acattttctt ttttccagta atgacaggaa
    18901 aacggtcttc tccgattggc acaacatcta cgttcgctaa cccacgtccg ccttcatcat
    18961 aaccgccctc gcattgattg gctgctgtag ctccgcccca taaaaaatct tttctgaatc
    19021 ccatcatact tcctccatta catgtgtatt gcttgataat tctcttgcca gttcatcttg
    19081 gacttcgatt ccttctgctt ttaaccggct gattgtttcc ttgaattgcg gtaaatactg
    19141 ctgatgtgca ataaacaact cattcatgat ccgttttgct gtctttcctg atcgtataag
    19201 cggattgatc gtaaacgctt gtaaagctgt tccataatca ccagtcacag ctgcttcgat
    19261 cgtcagcaat tccatattct tcattacttg caaccagcct ctttcagcag caggaaggga
    19321 accaaacgct acatttcgtg cgccttgtgc gccaacatag gcagtgactt ctactacaca
    19381 atctgccggc agatcaggaa cagctccatc attctttgta gataccacga tctgtgtttc
    19441 tttatttcca tagatcgaag caatcgtttc acaggcagca tctgaataat aagcaccacc
    19501 tcgtttttgc aattgttcag gtttatgatc caattctgga tctttgtata attcaaacag
    19561 ctctgcttct gtttctttca cttgctgcgc tcttgtacca atcgtttgat attcttctaa
    19621 agcatgttcc agcatttctt cttcacggta gtaatagcgg tggtagccac aagggatcat
    19681 gttcatttgt tttaattgtt ctttgaaaaa cgggatatca aagatatttt tcggcaatcc
    19741 attgtcttca tcgtataaac gatcaatcaa ttctgccgtg cggtcatttc ctttattatc
    19801 tgctactttg tgccaatgga agtgatttag accggcaaac ttgtagatca gctcttcttt
    19861 tttcttgcca agcattgttg gttctgtcat catcgccatt actggcacat tgcataatcc
    19921 tatcacttta tcccatttgc catatctgat aactgcttct gtcaccatcc cgcttgggtt
    19981 cgcgaaattg atcagccaag catttggaca taaacgcttc atgtcttcaa cgattgccaa
    20041 aaccactgga acggtacgaa aagctttgaa tatcccgcca gcaccatttg tttcttgccc
    20101 aatcatccca taataactag ggatccgctc gtctttgatt cgtgcatcta attgaccgac
    20161 acgaaactga gtcgtaacaa aatccgcatc tttcaatgct tcttcccgat ccagagttgc
    20221 atagattttg acttcatacg gagaagcatc ccacatacgc tgagacatct gactgacgat
    20281 ctcaagcttt tcttttcctt cttctacatc tacgagccag atttctcgta tcggtaattc
    20341 ctcatatctt ttgataaatc cttccatcaa ctcgggggtg taactgctgc ctccaccaat
    20401 cgtagcgatt ttgattcctt ttgccatgtt ctcactcctt tactttttac acttttatta
    20461 tcttttttgt aaacaaaaca aacaatagaa attcgtttct ttccagtaac atttacaaaa
    20521 gttagtgttt tctactggat ttttgtaaat atcttgtcaa aaaatgtctt tattaagcta
    20581 tataacataa atccgtttac aaatcatttt caaaaaagtt ataatttacg taaaaaaaca
    20641 agaagaaggt atctacatgt tattacgtga aaaaatcaaa gaaatctcct tttctccttc
    20701 agaaaatgaa gtgattcagt ttctcttaag cgattctcca gcacttggcg aaatgaccat
    20761 ccaagagatt gctgagcgat gttatgtcca cccgtccaca ctcatccgtg tagcaaaaaa
    20821 aatgggtttt gatggctggg tcagttttcg taatgctttt ttagctgaaa cagcctatct
    20881 tgaagggaat tttcaagacg tagatgcgaa tcttcctttt tccaccacag acggtattat
    20941 gacgatcgca aataaaatgg cttctttaga acagatgacg attcaagata cactttctct
    21001 cattcatcat gaccaactgc aaaaagccaa acaactaatg cttcaagcaa aaaaaatcgt
    21061 gattttcgcc agtaactcga atactttgat ttctcaagat ttcatgctga aaatgaagcg
    21121 catcaaaatg gatgtcacga ttgtcaccac ttttggtgaa tcgttctatg aagcgtacaa
    21181 ttgcacgagt gatatgtgcg ggattctgat ttcttatacg ggtgaaaaca atgtgatcct
    21241 tcgtaccgca gaaatattga aacaaaatca tgttccaatt ttagctttga ccagtatcgg
    21301 tgattcaacg ctatcttcaa tgagtacagc cgctttgcga cttacgacac gcgaacgact
    21361 gtattcgaaa attggtaatt ttacgatcaa tacatcgatt tgttatcttc tagacgtgtt
    21421 atatagttgt gtttttgcag aaaattatca acagaattta gtccatctaa tttctgtagg
    21481 acaaaaattt gataaacgcc ctatcagctc agccatcatg aaagagcctt tcaattttga
    21541 aaataaagaa gtggactaaa aagaataatt aagcctagtc cattctttca cctaaccgtt
    21601 caggtcagtg cggtgataag tgcatcgtct ggcccgaccg tctccccttg attttccaaa
    21661 atatccaaat aatccgtgta attggtgacg atcacaggtg tttctaaact gtatccttcc
    21721 tcttcgattt tctttttgtc gaagcggagc aacagatcgc cttgtttcac tctttctcct
    21781 tgctgcacca agctttcaaa atatttccct tccaactgaa cagtatccaa tccgacatga
    21841 atcagcaatt ccagacctgc ttcagaaacc attccgattg catgtttcgt cgggaacagg
    21901 gtcatgactg ttccgtcaaa aggggcacgt acttctcctt tttcagggta gatcaagatt
    21961 cctcgaccta gtgttccttg tgcaaaagct gcatcctttg ctgcattcag tggagcgatt
    22021 tgtccttgta tcggtgtttt tacaatttct ttacctgatc ttttctgtct atcatctgtt
    22081 tcacctgctt cctccttcca aaagaaaaaa gtcagcgcaa aggataccac catcgcaatg
    22141 gcgattccaa tcaatacatt aatcacatcc gttaaatcac catctggtct aatatagttg
    22201 ggaattgtaa agatgcctaa tccacctgga ttccaagaag ttaaattcat taatccacta
    22261 tacgcgcccc ctaacgcacc accgacacag gtaaaaataa atattttttt acgtggcaat
    22321 gtaaacccat aaatagctgg ctctgtaatc cccacaatac cagaaacaat ggctggtatt
    22381 gcgattgctt tgtcttttgg gtttctaagc ttaaagtaga gtgcagtaag tgctgcagtt
    22441 tgtccaaaag acactactgc aacaggtgtc atgatatttt gccaaccatt cacagaaaat
    22501 tgcatcaatc ccattggtac gattgcccaa tggagaccaa acatcactag aatttgccag
    22561 aagaagccta cgattgctcc ataaataact ggcgaaagtg ctaaaagtgt ttcaaatcca
    22621 gccattaaaa tatcagttgc gaatgtgaat gctggtccaa cgagtaaaaa acctaagggc
    22681 attgtaataa gaacggtgaa gaatggaacg aagaaattcg cgatcatctc aggtactaac
    22741 tttttaattg gtttctgaat ttggctagct aacaacacaa taaagataat gggaatgaca
    22801 gaagaagcgt agttacgcgc aatccaggga atgccgaata ctgtttgata aataggtgct
    22861 tcaaaaatag tgccagaaaa tatggtagtc agtggtgctt ctgctgcacc actcaaagct
    22921 tccagcgaca agctaggatt cattaatgct gccccaagca acatccctac gaattcattt
    22981 actttaaatt tacgggcagc tgttaaaccg ataataacag gcataaattg gaatacagaa
    23041 tcaccgatat tatcaaacat aacaaatgtg ccagaaccgc ggtcaaatgc gcctaagaag
    23101 acaagtaatg ctgtcagtcc acgaatcatc ccaccagcag ctagaatagc aaggaatggc
    23161 tggaaacagc ccgatattcc atcaataagc atatcaaacg gtctcatttt ttctgttgaa
    23221 acagcctcat caaaagtaag gtttcctgct tttataacct cttcaaacac gactggcaca
    23281 tgattcccga tgacaacttg gtactgtcct cccgctcgca ttaccgtcac aacaccgtcc
    23341 atatttttca agatctcatc atttgctttt tcttcgtttt ttaatttgaa acgaagcctt
    23401 gtgatacaat gtgtcaaacc attgatattt tccttcccgc cgacattttc tacaattttg
    23461 ctcgctaatt cttgatattt tcccatttct attccctcca atttattagt ggaaggttta
    23521 gccaactgaa tgtcactacc cttatttcat gcgttcttgt taatttatcc tccttttttt
    23581 atttatttac aacgattctc atgcactaaa cgcgcaatat ggatcgttaa atacattttt
    23641 tcgtccgtgg acatctggta gttgaatatc tcggctaaaa agtctgctat tttctcaacg
    23701 caaccaaacg tatccttata ttttttttga ataacctggt acaattcctc agaaacttct
    23761 cccacttctg atttttctcc atctaggacg cgtgtcagaa aaaaacgtag gtgtgtgacg
    23821 aatcgaaaaa aatggactga atcttcatca aatgtaatct tatagtaata gcggacgata
    23881 tttagaatat cctgtattgt tgtagtaaac aggtaggtat tgtcttgttg gctttgaatg
    23941 ccttgtgcat tgactaaatg caatgcgata aatccggctt catcttctga taacttgatg
    24001 tggtatttat ccgagatttt cttcactgtt tttttcccga tggcatactc tttaggaaat
    24061 agttgtttga tatcatacag caatacattt ggtacaaata ttcccatttt gtgacgttcg
    24121 atagccgtat acaaatgatc cgtcaatgca atatacaccg aatcattcaa ctggacttgt
    24181 agagcctctt ttgtatattc aatgatttca caagaaattt ctaagtactc aagaggaatc
    24241 gcttgtgcca attcttggaa ctgttgtgaa agtccgtcat ttgtcaactg aaattttttg
    24301 tcgatgcatg agtcaaaaat cgggtcaccc actttctttt tgaatgccaa accttttccc
    24361 atcacgatca tttcctgatt tctttcatcc ctggttatta cgacgttgtt attcagtact
    24421 ttctcaatca gcatgtttta ctcctttcca tgatatatag ggctgtatcc aaaacaaaaa
    24481 gaaaaaccca aaaagccgtc catggaacca tgggagactc atcaggttta gcttgcatca
    24541 ggcaatcact atccaagttg tatcgttttc tttatattat cgaaagcaag cacttttgtc
    24601 aacgctttct tagcggctat tgggcacttc tgactagctg tcgtgatttt ttttaaatct
    24661 ggtaattacg acactaacgc tcacagatga agaatctggg aagctctgtt ttattttcac
    24721 tctagcgctt aggtcaaaat gctactatgc tactgtccca acttctaata tacggtgttc
    24781 cagtaccagc ttatcggtga taagccaaat ttatgcaaaa atctggaaag ccttttttac
    24841 aatttttttc ttatcaccta aagctgaacg gagccctgtc acaaccactt tttacacacc
    24901 actgctttgc agtttgtaca tatctgcata caaaccattt tgcgctagaa gtgtttcatg
    24961 attcccacgt tctactatat gacctttgtc taaaactaaa atcaattctg catcccgaat
    25021 cgtggacagc cgatgggcaa tcgcaatcgt tgtccgtcct tgtcgcattt ttgtcaatcc
    25081 ttcttggatc aatccttctg tttcagtatc gatatttgct gtcgcttcat ccaatactag
    25141 tattttagga tctgtcacga ttgttcgcgc aaaagtgatc agctgtcgtt gtccgctaga
    25201 atagctggca ccgtgttcga tcacttttga gtgatatcct tttggtaatt catcgatgaa
    25261 cttgtttgct tgaacaaact cagctgcatg gcgaatctgt tcgtctgtga tagttggatt
    25321 catcatccgg atatttcctg cgatatctcc ataaaacaag aaggcatctt gcaagaccaa
    25381 tcccattttc ttacgtaact cttccattgg atattctcga atgtcatgat catcaatcaa
    25441 gatctggcct tcataaaatt cgtaaaaccg catcaatacg ttgatgatcg agctttttcc
    25501 gcttccggtg tgccccacta atgcaacggt ctctcccgga ttagcgacaa aagagatatt
    25561 gttcaataca ttgtgctctc catcataaga aaagcttaca ttgcgaaact cgatcttacc
    25621 tgaagtaatc gcttgatctg ctccaggatt ttgttgcggt gcatattctt ctgtatccat
    25681 gattttcaaa atacgactac cggcaaccat tccatctgta aaggtgctca aaaagtccat
    25741 catttgagac attggattaa aaaatccctg gacataggtc gtaaatgcat agatcagtcc
    25801 aacttctacg ggagaatgca gcgcatcgat cccaaacaaa gtcaacgcca acgcaatcgc
    25861 caatgcatat agaaaattga tgatcggact taacagaatc gaatttgttt tgatcatcgc
    25921 atagcgcgtg tctaaatact cgttgttcac ttgttcaaat tctttagcta aacgagcttc
    25981 ttggcgaaac tgctggataa tacgcatgcc ggaaatcgat tcattcaatt tcgtgttcag
    26041 ctgacttaat ttttcccgca tattccggta aatacgagaa ctgaatttct ggtaatacca
    26101 gatgacgatt cctaaaatcg gcaagaacac caaagtccac aaggcaattt ctccattgat
    26161 ttgaaacatg gcaatgaagg aagaaacgac tgcaaagatc ccagtcaata ccatcaaaaa
    26221 gacataccaa aattcaaata gcgtttctgt gtcatttgtc actcgagaaa cagtcgatcc
    26281 tgccggtgtc tgatcaaaat aacgcatacc tagtgtatgc agcttttcaa ataatcttac
    26341 ccgaacatac tgataggtct tcaatgaagc catggaatac aaataccatt ggaaaaacca
    26401 aatgatactt ttgactatga ctccaaacaa gtacatacca gcaaaaaaca aaataacttg
    26461 tgtcgtagcc gactgtttag ccagatgatc atccataaaa gtttggatga tccttggcaa
    26521 taggacattg atcacagaca gggcaaacgc aaataagata gcaacgatga acgttcttct
    26581 aaaaggtttg gcgaacttca tcagtcgttt gatgatcttc ttctgttctt taagtggaat
    26641 cgattttgtc cattccgatt gataattttc atccatcagg cctcgcctcc ttctatcttc
    26701 gcttctaatt tctgtttctc ccacattcgt tcataccagc cgcctaatgc aagcagctca
    26761 tgatgggttc ctcgttcaat gattttccct tgatcgacaa caatgatttc ttttgcatgc
    26821 atcacgctgc ttaaacggtg agctgcaatg atggttgttt tctcttttct tatttctttt
    26881 aaattatgca agatagcttc ttctgttttg gcatctactg cagaaagtgc atcatctaag
    26941 atcagcaatt caggattttt gatcaatgct cgagcaatcg aaatcctttg tttctgtccg
    27001 ccagataaag agactccacg ttctccaact aaggtatcat aaccttttgg taattccttg
    27061 atttcattgt tgataaatgc taaagaagcc gcatgctcta cttcttcttc tgaaagtttt
    27121 ggattagaaa aacggatatt atctcgaatg gtcatcgaaa acaagaagtg atcttgaggc
    27181 acatagccga ttgatcctaa taaagcattc aatgaatatt ctttgatcga acatccgcca
    27241 aattgaattt ttccttgata atcatcgtat tcacgcatca gtaatttgat gattgtcgtt
    27301 tttcccgcac cagttttccc aacaatacct aatgtttctc cttcttggat cgcaaatttg
    27361 atttgttcta atgctggtga ttcttctttc ggatagttaa aagaggaaat agctacattc
    27421 agctcgcctt ttgccggagt ctgcactgca cccagttgtt ccacgatatg cgtcttttca
    27481 tgcaaaagtt cattgacgcg atcataagaa gcatttcctc tttccaatac gttgaacaat
    27541 cgaccaatcg caaacatcgg ccaaacaagc ataccgatat agctaataaa tgagaccatc
    27601 tgcccgattg agatcgtgcc ttccaagaca tactttccac ctaaaataat tgttgcaaca
    27661 taagacagac cgataatcaa ggtaatgaat ggatcaaata aggagtctaa aaaattgact
    27721 cgtttgtttt taataattgc atcttcaatc ttttcttcaa aatccgcgag atcttctttt
    27781 tcttgtccaa atgttttaat gactttgatc ccagtaatac tttcttgtgt cttatcattg
    27841 atactagaaa aagcttcttg cgaatcacga aaagcatcgt ggagttttga ccccagcact
    27901 cgtgaagtaa cggctagtaa aggcagagga atcagcgcaa tcagtgttaa gcgccaatca
    27961 ataaacaaaa tcatcgcaat gatcgttgtt ccgcccgtga tcactgaatc tgcaaaggtc
    28021 aagataccag ctcctgctac attttggatt gcattcaaat cattcgttgc atgagccatc
    28081 aaatctccag tccgatattt ttggtaaaag agattgtcca tttctgtaaa atgtttgaat
    28141 agctgctgcc gaagttcttt ctccaaccga gctgcacttc cccaaatatt cgtccgccag
    28201 atataacgaa aaagatattg taagagagct gcaaggatca ataataagat ccaccaggaa
    28261 atgatcttta ttgataaatt tttatcggcg atctcatcaa tgatgatccc aatcactttt
    28321 ggaggagcta actgtaccaa ggccactgcg aatagggaga aaatcccaat taaataatgt
    28381 ttcttttcct gtcgaaaaaa ccaacctaat tttctaaaaa tcgacatttt actcctcctt
    28441 tgtctttatc aagagcatag gattgtctgt gcttgtcttt tttgctgaat agtctgaata
    28501 gtctaacaaa ttacgaaata aaaaaaggac tgaaaaaatc agcccgcaaa aagtctttgg
    28561 gtcagaacct attttttccc ttggttcttc atttgctgca tcatctggcg aattttcttc
    28621 tcagatggtt tttgccccat tgacatcatc attgatcgca acatgtcctc attaacggga
    28681 ggattttttt ctaaatagtc tttcatatat ttacgcgcta ggaaaaagcc acctactgcg
    28741 ccgattagca aagcaataat agcaataagt acgacaatac ctgttgatac catataaatc
    28801 tctcctttct atttccgaat tgctctcaat gatttatttt actaaagttt attgaaaaaa
    28861 gaaaggcgta ttctttagtt tcatgcaaaa aatagtggtt tttttagaga agaacaaaat
    28921 ttctttcatt aaccagcaag acacgccacg aaaaaaaatt aggcatacca aatctttttc
    28981 tttaattttt tctttctgag gtgtagaatg taatcgtaga taaataggaa aggtggtttt
    29041 ttcttgaata atcaatccaa caaacaattt cataagcatc gacttattga acatacaaat
    29101 ggtttatctt tagctgaagt aaatggaaca gtggatgtac ccaagggaaa aagttttttt
    29161 aaaacacttc ttgcttactc tggaccaggt gcattagtag cagttggtta tatggatcca
    29221 ggtaactggt ccacttcgat cactggtgga caaaatttcc aatatttatt gatgtcagtt
    29281 attttgatgt ctagtctgat tgctatgctt ttacaatata tggcagcaaa attaggaatc
    29341 gtgacccaaa tggatttggc ccaagcaatc cgagcacgga caagtaagac attgggtgtc
    29401 attttatgga tcttgactga attagccatt atggcaacag acatcgcaga agtcattggt
    29461 gcagcaatcg ccctctacct gctttttgat attccattgg taattgccgt atttatcact
    29521 gtgttagatg ttttcgtcct tttattgctg acaaaagtcg ggtttcgtaa aatcgaagct
    29581 ttggttgtct gcttgatttt cgtcatttta ttcgttttcg tctatcaagt tggcttgtct
    29641 aaccctgatt ggcacagttt gtttgccgga tttatcccta acagtcacac attctcagac
    29701 tcaccggtca tcggcggcga aactccgctg actggagctt tagggattat tggtgcaaca
    29761 gtcatgccgc ataacttata tcttcattca gcagtttctc aaactcggaa gatcgatcac
    29821 caagacgaag aagatgtagc acaagcggta cgcttttcta cttgggattc taatatccag
    29881 ttgtcaatgg cttttctagt caatgcactg ctattgatca tgggtgtggc tgtattcaaa
    29941 accggttctg tccaagatcc ttcctttttt ggtctgtatg aagctttatc tgatccatca
    30001 gctatgagca atggtatctt gattgctgta gcaaaatctg gtatcttgtc tactttattt
    30061 gctgtagcac ttcttgcttc tggccagaac tctactatta ctggtacgtt gaccggtcaa
    30121 gtcatcatgg aaggcttcat ccatatgcgt atcccaattt ggctaagaag actagtcaca
    30181 cgattgcttt ctgtgattcc tgtattgatt tgtgttttgt ttacaagtac aaaaggaacg
    30241 atcgaagaac ataccgcttt gaataacttg atgaatgaat cccaagtttt tctggctttt
    30301 gcattgccat tctcgatgat tcctttactg atgatgacca atagccaagc ggagatgggg
    30361 agccgattca aaaattcatt tgttgtaaaa ctacttggct ggttctctgt gattagtctg
    30421 acttacttga atttacgagg attaccaggt caaatcgaaa gtttcttcgg cgagcaagcc
    30481 acctcagctc aactagcatt ggctgatcaa atcgcttatg taatcattgt tcttgtcctt
    30541 ctcctgttgg catggacgat tggtgattta tataaaggaa acaaaaaata tgaacagcgt
    30601 ttgcaggaaa cgatagaaga aatcgagtag aaactgattc ttttacacaa aggaggttgt
    30661 gtcagaagtg ggcagcttca agaaataaga aggaatttaa gaaaattgct tttcaaattt
    30721 ttgtgaaatt tcggcttatt tccgaagaag ttacttctgg aacaccgtgt at
//
LOCUS       DBNICI010000013        30445 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.013, whole genome shotgun sequence.
ACCESSION   DBNICI010000013 DBNICI010000000
VERSION     DBNICI010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 30445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.013"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            227..715
                     /gene="purE"
                     /locus_tag="AC1ZCC_000500"
     CDS             227..715
                     /gene="purE"
                     /locus_tag="AC1ZCC_000500"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIQ1628779.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            708..1832
                     /gene="purK"
                     /locus_tag="AC1ZCC_000501"
     CDS             708..1832
                     /gene="purK"
                     /locus_tag="AC1ZCC_000501"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HIQ1628780.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            1845..3140
                     /gene="purB"
                     /locus_tag="AC1ZCC_000502"
     CDS             1845..3140
                     /gene="purB"
                     /locus_tag="AC1ZCC_000502"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIQ1628781.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(3262..4650)
                     /gene="ltrA"
                     /locus_tag="AC1ZCC_000503"
     CDS             complement(3262..4650)
                     /gene="ltrA"
                     /locus_tag="AC1ZCC_000503"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HIQ1628782.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(5219..5596)
                     /locus_tag="AC1ZCC_000504"
     CDS             complement(5219..5596)
                     /locus_tag="AC1ZCC_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628783.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            5852..6580
                     /gene="purC"
                     /locus_tag="AC1ZCC_000505"
     CDS             5852..6580
                     /gene="purC"
                     /locus_tag="AC1ZCC_000505"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIQ1628784.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            6580..6834
                     /gene="purS"
                     /locus_tag="AC1ZCC_000506"
     CDS             6580..6834
                     /gene="purS"
                     /locus_tag="AC1ZCC_000506"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HIQ1628785.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            6836..7507
                     /gene="purQ"
                     /locus_tag="AC1ZCC_000507"
     CDS             6836..7507
                     /gene="purQ"
                     /locus_tag="AC1ZCC_000507"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HIQ1628786.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            7508..9730
                     /gene="purL"
                     /locus_tag="AC1ZCC_000508"
     CDS             7508..9730
                     /gene="purL"
                     /locus_tag="AC1ZCC_000508"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HIQ1628787.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            9715..11154
                     /gene="purF"
                     /locus_tag="AC1ZCC_000509"
     CDS             9715..11154
                     /gene="purF"
                     /locus_tag="AC1ZCC_000509"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIQ1628788.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            11177..12229
                     /gene="purM"
                     /locus_tag="AC1ZCC_000510"
     CDS             11177..12229
                     /gene="purM"
                     /locus_tag="AC1ZCC_000510"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIQ1628789.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            12226..12804
                     /gene="purN"
                     /locus_tag="AC1ZCC_000511"
     CDS             12226..12804
                     /gene="purN"
                     /locus_tag="AC1ZCC_000511"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIQ1628790.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            12801..14336
                     /gene="purH"
                     /locus_tag="AC1ZCC_000512"
     CDS             12801..14336
                     /gene="purH"
                     /locus_tag="AC1ZCC_000512"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIQ1628791.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            14352..15626
                     /gene="purD"
                     /locus_tag="AC1ZCC_000513"
     CDS             14352..15626
                     /gene="purD"
                     /locus_tag="AC1ZCC_000513"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIQ1628792.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            15748..16662
                     /locus_tag="AC1ZCC_000514"
     CDS             15748..16662
                     /locus_tag="AC1ZCC_000514"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIQ1628793.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(16795..18360)
                     /locus_tag="AC1ZCC_000515"
     CDS             complement(16795..18360)
                     /locus_tag="AC1ZCC_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIQ1628794.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(18353..19804)
                     /locus_tag="AC1ZCC_000516"
     CDS             complement(18353..19804)
                     /locus_tag="AC1ZCC_000516"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIQ1628795.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIRESKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(19818..20834)
                     /locus_tag="AC1ZCC_000517"
     CDS             complement(19818..20834)
                     /locus_tag="AC1ZCC_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIQ1628796.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            21054..22142
                     /gene="chvE"
                     /locus_tag="AC1ZCC_000518"
     CDS             21054..22142
                     /gene="chvE"
                     /locus_tag="AC1ZCC_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HIQ1628797.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            22196..23722
                     /gene="mmsA"
                     /locus_tag="AC1ZCC_000519"
     CDS             22196..23722
                     /gene="mmsA"
                     /locus_tag="AC1ZCC_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HIQ1628798.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            23724..24929
                     /gene="mmsB"
                     /locus_tag="AC1ZCC_000520"
     CDS             23724..24929
                     /gene="mmsB"
                     /locus_tag="AC1ZCC_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HIQ1628799.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            25186..26163
                     /gene="guaC"
                     /locus_tag="AC1ZCC_000521"
     CDS             25186..26163
                     /gene="guaC"
                     /locus_tag="AC1ZCC_000521"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HIQ1628800.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            26427..27851
                     /locus_tag="AC1ZCC_000522"
     CDS             26427..27851
                     /locus_tag="AC1ZCC_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HIQ1628801.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(27893..28150)
                     /locus_tag="AC1ZCC_000523"
     CDS             complement(27893..28150)
                     /locus_tag="AC1ZCC_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628802.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            28312..29421
                     /gene="pdhA"
                     /locus_tag="AC1ZCC_000524"
     CDS             28312..29421
                     /gene="pdhA"
                     /locus_tag="AC1ZCC_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HIQ1628803.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            29424..30401
                     /locus_tag="AC1ZCC_000525"
     CDS             29424..30401
                     /locus_tag="AC1ZCC_000525"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIQ1628804.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         9647 a         5337 c         6803 g         8658 t
ORIGIN      
        1 agcttctcat gtttgttgaa tattgttcct tgtgaccgag gagttggcag gagaacaccg
       61 tttatccata aaaaggaatg tgacctaact tttgtcacat tccttttttt tatctttaat
      121 aaacacgaac aatgaaatgt ttttctttgt taacattcga aatttctttg acatcaaaaa
      181 aagtgattgt taaaatatga atgtagaaaa actgggagga aaaacaatga cagcaaaaat
      241 ctctgtgatt atggggagta cttcagattg ggaaacaatg aaagaagcat gtcaaatatt
      301 ggatgaattc ggtgtgtctt atgaaaaaaa ggtcgtttct gctcatcgaa ccccagattt
      361 gatgtttgaa tatgcacaaa acgctcgaaa aaaaggaatc aaaatcatta ttgcaggagc
      421 agggggagcc gcgcatctgc cgggtatggt cgcatcaaaa acaactttgc cggttatcgg
      481 tgtaccggtc caatcacgaa cattaaatgg tttggattcg cttctttcga ttgtccaaat
      541 gcctggagga gtacctgtag ctactacagc aatcggaaaa gcgggagcaa ttaatgctgg
      601 actacttgcg atacagatgc tttccatgta tgatttagaa ttggaggcta aattggctga
      661 aagacgttca atgttggcta aaactgttat agaaagtagt gatcaacttg gttaaacctt
      721 tgttgcctgg ttcaacgatc gggatcgtcg gtggcggtca attaggtcgt atgatggcga
      781 ttagtgcaaa agaaatgggc tttaaagtag gtgtgctgga tcctgtgagc gattgtccag
      841 ccgcacaagt tgcagattgg cacattgaaa gtgtctatga tgacaccttt gcgctgaaag
      901 agcttgccag aagaacagat gtcattacct atgaattcga aaacgtcagt gtagaggcgt
      961 taaatgcgat tttacccatg tcatttattc ctcaagggac agacttgctt gcgattacgc
     1021 aagaccgcct gttagaaaaa tcttttttgg aaacgaataa catcgtgatt gctccttatg
     1081 caacgatcgt cagtccgacg gatatccaag atgcgattga cgggatcggc tatccttgtg
     1141 ttttgaaaac cactcgaggc ggctatgatg gcaaggggca gtatgttttg aaaagtcggg
     1201 cggatctagc tcctgccatg gaccttttaa gagaaggaac ctgtgtttta gaagcatgga
     1261 tcccgtttga aaaagagatt tcgatcatgg tggcaggaaa tggtcaagga gatttcacga
     1321 cttttccggt cgtggaaaac atccaccaca acaatattct tcatgaaacg attgctccag
     1381 ccgcgattga tcaggatgtg atcgaggagg ctgagcgtat tgcccgtgtg attgcagaag
     1441 cagtgagcct ttcaggtgtt ttaggggtag aaatgttttt aacaaaaaca ggcgggctat
     1501 atgtgaatga attagctcct cgtcctcaca attctggaca ttattctatt gaagcttgtt
     1561 ctatgagtca gttcgatgcc catattcgag gtatttgtgg ttggccatta ggagaaaaag
     1621 tacgtttgat aagcaatgca gtaatggtca atgttttggg agaacaactg ctggaaagct
     1681 atcgcttgat tgagaataag ccagactggc aattccattt ctatggaaaa aaacaagcaa
     1741 aaaaaggtcg caagatggga catatcacta tcttgacaga agacatccag caaacgctgg
     1801 aagaaatcaa acaaacgaat atttgggatt agaggagaaa aaacatgtta gaacgttata
     1861 cacgccctga aatggggaat atctggacgg aagagaacaa ataccgtgcg tggctcgaag
     1921 tcgagatttt agcagatgaa gcttgggcag aacttggtga aatcccgcaa gaagatgtga
     1981 aaaaaattcg tgagcatgca tcatttgata tcgatcggat cttggaaatc gaaaaattaa
     2041 cacgccatga tgtcgttgcg tttacacgag cggtttctga aacattggga gaagaaagaa
     2101 aatgggtcca ttatggtcta acaagtacgg atgtcgtaga tactgcttat ggctatctca
     2161 tcaagcaagc aaatgatatt ttacgggaag acttgaaacg ttttacggaa atcattggcg
     2221 aaaaagcaaa agaacacaaa tatacagtga tgatgggtcg gacacacggt gtccatgctg
     2281 aaccaactac ttttggattg aaacttgcca catggtattc agaaatgaaa cggaacatcg
     2341 aacgatttga acatgcggca aaaggagtag aagcaggaaa aatctcagga gcagtgggaa
     2401 cgttcgcaaa tatcccgcca tttattgaag agtatgtatg taaacaattg gggatccgtc
     2461 cacaagaaat ctctactcaa gttctccctc gggatctaca tgctgaatat ttctcagcga
     2521 tggcgcttat tgctacaagt attgaacgtt ttgcaacaga gatacgcgga ttgcaaaaat
     2581 ccgaaacacg tgaagtagaa gaattttttg cgaaaggtca aaaaggttct tctgctatgc
     2641 cgcacaaaag gaatccgatc ggttcagaaa atatggctgg tctagccaga gtggtgcgcg
     2701 gacacatggt cactgcctat gaaaatgtca cattatggca tgaaagagat atttctcatt
     2761 cttcagcaga acgaatcatc ttgcctgata ccacgatttt attgaattac atgttaaatc
     2821 gttttggcaa tatcgttaaa aatctaacgg tctttcctga aaacatgaag cggaatatgt
     2881 ctgcaacatt tggtttgatt tatagccagc gcgttatgct gaaattgatc gataagggaa
     2941 tgacaagaga agaagcttat gatcttgttc agccaaaaac tgcgtacgct tgggacaacc
     3001 aagtgcagtt ccgcccgctg ttagaagaag atccaaccat cacatcggta ttatctaaag
     3061 aagaaataga cgatgccttt gattatcatc atcatttgaa aaacgttgac gtgatattca
     3121 aacgagtagg tttggaataa agaagagtga gttgcaaaat tcaattaaaa tagaatagca
     3181 aaaataatcg agtagagggg aaagccaact gaccttccgc ccctctcaca ccaccgtacg
     3241 tacggacccg tatacggcgg ttcaataagt tgagtgtacg aactggtact tcaaggagat
     3301 gtcctcatat ccttggcggg ccagttcttt gtctgttaat gatcgatgaa gaatatgact
     3361 tttggagact gaccaaatac cttttcttgt atttgaccat tcatacgctt tcgctttatc
     3421 gatccctaaa cgaatcagat tttttctttt ggttcttgga ttcttccatt gtttccaaat
     3481 atattgtctg attcttcgct tcaaccaacc gtttaactcc ctcatgaatt ctttcatctc
     3541 accaattcca tagtagttga tccaaccagt cattagttgc ttgatttctt ttaaaataag
     3601 ttggatactt cttccacggt ttcttttagt gagttgtttg agcttgcttt tcacacgctg
     3661 ttttgctttt gcgtgagggc gaattttaat acctttcttt gttggcagaa tgcagaatcc
     3721 aagaaacttt cgtttcaatg gacttcctac cgcactcttt tctcgattta ccgtcaactt
     3781 tagttgttct tcgagaaatt gaatggattt cttcatcaca cgctgtcctg ctcgtttgct
     3841 tctaacatag atattgcagt catcggcgta tcgaacaaat ttatgtccta gattttctag
     3901 cacttgatca aattcgttca ggtagatgtt acttaacagg ggagagagat tgcctccttg
     3961 gggcgttcct tcttcagttg ttgtgatgag tccattttcc attacaccac tcattaagta
     4021 ttgacggatc agttttagga cttgtttgtc ctttactttc cgctcaatga aatacatcag
     4081 cttatcgtga tttacggtgt caaagtaggc tttcatatca atgtccacca catatcggta
     4141 accttgttca taataagatt ttgctttctt cactgcgtca tgtgcgctgc gatttgggcg
     4201 aaacccataa ctattatctg agaattctgg ttcaaatagc ggttgtaaca cttgattgat
     4261 tgcctgttga agcatacgat caataaccgt gggaattcct agttttctta caccgccgtt
     4321 tggtttcggt atttctactc gcaaaacagg ctgaggttta tagttccctt ggcgtaaatt
     4381 ggatagtaat tcctcgcggt gctctttcaa gtaaggaagc agttgatcga cggtcatccc
     4441 atctgcacca gccgcccctt tatttctgac tacttgaaga tacgcttgat tgagattatt
     4501 ccgatctaga attctcccaa acagattttc tacaccatct tgtttcctct gttcaccgca
     4561 agaagtgctg cgcacttcat catacccttc gtgttccaca ctagctcttt gatgatagcc
     4621 aagttttctt gttttctgcg gtcgtcgcat cttctcacac ctcctaaatt attcaagatt
     4681 cttattgttc agtccttcgt aacggtcagt gtcgttacta ctatgacctc ggctgacttc
     4741 tcatgattca ccatcatgtc gccatgatcg ttgtgctggt ttgttttcat tcaacgccca
     4801 caacaccctc atgagatctc ccccggtaag agtgaaaact ttcctcccat gtaactgcta
     4861 gatttactgt ataggtttcg ggcagtattg gactttgtct tgtgatgaag acttatccga
     4921 cctaattcag ccttgtatct agtttctgtt cgtcagttca ggagtttgcg tccgacttcc
     4981 ttcagccaca acctcacgat tgccgccttg tctttcgcta acagttccta ctgccaagcc
     5041 tgtagtggac tttcaccacc aagttttcac ccatgcaggg cgcacaccaa aaaagaagca
     5101 gatccgttag cggttctgct tcttttagtt tattatattt tcttatcaat aataggaacc
     5161 tgattttaaa agcggctacc tctttaccgt gcctctttac atacaaaaaa tagaggaatt
     5221 aagcgtaaat ggcttggcta ttataactaa aatgctggtt atggagatgt tgcagcaagt
     5281 tttcgagaaa agcgtattcc atctcttgtc cattgaataa gatctggata cgataatgat
     5341 ttttgaaacg cttctgttca tgaatctgac tgattgaaag ttcgcctttg tgttctgtct
     5401 gataaagaaa agtcctttta cggcattcag cacagcgctt ggttgctcgg cggatttttc
     5461 ggaatgtata aaaactagta tctttattgc ataaataaac gtctttgatc gacagcaaat
     5521 cattatatag acaggaacgt tcttcagctg ttaattctga taaaaaaagc tgaccttgtt
     5581 ttattgcatc ttccatcggc aagtgcctcc tcgaacccat tatccaaatt tttagttaat
     5641 tctaatatac catcattaag tataaatgta aacgcttgtt gatggtataa gattttcgtt
     5701 tctgataaaa cacgaacatt aaaagaatct aatgccttaa atgtacatat ttttgttgat
     5761 ttataaaaag atgattgcta aaatttaaag tgaaaaaacg aacaaaaaac gatgtatttg
     5821 cttttaatgt aaataaaaag gagagacgta catggagaag aaagaacttt tgtatgaagg
     5881 aaaagccaaa aaaatatatc ttacttctga tgagaacttg atttggatag aatatttaga
     5941 tcaagcaact gcgttgaatg gcgcgaggaa agaccagata agtggaaaag gagaattgaa
     6001 taatcaaatc acaagtgcaa ttttccgtta tctgactcaa agaggaataa aaaatcattt
     6061 tattgaacaa ttgtctaaaa ccgaacaatt agcaaaaaaa gtacaaatta tccctctgga
     6121 agtggttatc cgaaatattg cagcaggaag tttctcaaaa cgtctaggtg ttcccgaagg
     6181 taaagaattc atcaaaccaa tcattgaatt ctactataag gatgaccagt tggatgatcc
     6241 atttatcaat gaagagcata tctatttctt acaacttgca aagccggaag aaatcagtga
     6301 gctgaaagcc caagcgttgg ctattaatga aattttgacc gaattatttg ctaaggtcgg
     6361 tttggtttta gtagatttca agctagaatt tggccggtcg aatggacaaa ttttgcttgc
     6421 ggatgaagta acaccggata cttgccgatt atgggatgaa cgtacgaatg aacatatgga
     6481 caaagacgtt taccgcagag atctaggtga tttgatccca gtctatcaag aagtatataa
     6541 ccgtctatcg gaactaactg aagaaaagga gcgagtatga tgtatttcgt aaaagtatat
     6601 gtaacctata aagattcagt gttagatcct caaggggagg cagtaaaaaa agcagtccat
     6661 cgtatgggat atgaaacaat cgaagatatc cgaataggaa aatactttga gatacaactt
     6721 gctgatgcag aagaaccaat cgatacagtg attgaaacga tttgtgacaa gctgcttgca
     6781 aatgtaaata tggaaacata ccgatatgaa atcaaaaaaa tggaggaagt ataacatgaa
     6841 atttgcggtc atcgtttttc caggttcaaa ttgtgacgca gatatgttat gggcagttcg
     6901 cgagatcatg ggcgctgatg ccgaatttgt acggcatgat gagtcctctt tagaaggatt
     6961 tgatggggtt ttactgccgg gaggattctc ctatggtgac tatttgagat gcggtgcgat
     7021 tgctcggttt tctacaatca tggaagaagt gattcgtttt gctgaagaag ggaaaccagt
     7081 attcggtaca tgcaatggat tccagatatt gacagaagca ggacttttgc caggtgtact
     7141 gagacgaaat gagtctttgc attttatttg caaaactgta ccattgaaag tcaaccccaa
     7201 gacggccttt acttcagagt atgaagccga cgaaataatc tatcttccag ttgctcatgg
     7261 ggaaggaaat tatttctgtg acgaagaaac acttcaagaa ctaaaaaaaa ataatcagat
     7321 tgcctttact tataatgaca acatcaacgg cagcgtggaa aacattgcag ggattacgaa
     7381 caaagcaggg aatgtgttag gaatgatgcc tcatccagaa cgtgcagtag aagacttgct
     7441 aggatcagaa gatgggaaac ggttctttgc ttccatttta aaaaacttcg gaaaggtgac
     7501 cgtatagatg aaaacattga aagaaccgac agctgaagag atcaagaaaa aaagaatcta
     7561 tgccgaatgg ggattgactg atgaagaata tcgaatgatc gaagaagata tcttaggaag
     7621 aatgcctaat tatacggaaa ctggactgtt ttctgttatg tggagcgaac attgttccta
     7681 taaaaattct aaaccggttc tacgcaaatt tcctactagt ggacctcatg tgttgcaagg
     7741 ccctggagaa ggcgcaggca tcgtcgacat cggtgatgga ctagcagttg tcttcaaagc
     7801 agaaagtcac aatcatcctt cagccgtgga gccttatgaa ggagctgcga caggagttgg
     7861 cggaatcatc agagatattt tcagcatggg ggctcgtcca attgcgttgc tagattcatt
     7921 gaggttcggc gaattagata atccaagaac gaaatattta ctagaagaag tagttgcagg
     7981 tatcggcgga tacggcaatt gtatcggcat accgactgta ggcggcgaaa ttgcatttga
     8041 tccttgttat gaaggaaacc cattagtcaa tgcaatgtgc gtaggtttga ttgaacacaa
     8101 acatatccag aaagggcaag ccgctggcgt cggtaattcg atcatgtatg taggtgcaaa
     8161 aacaggtcgc gacggtatcc acggagcaac ttttgcctca gaagaattta atcaagaaga
     8221 agaacagcaa cgttctgctg tacaagtagg cgatcctttt atggaaaagc tgctgcttga
     8281 agcgtgtttg gaattaatct tgcatcattc ggattctcta ataggtatac aggatatggg
     8341 agcagcagga cttgtttcat ctagtgcgga aatggcttca aaagctggct caggacttat
     8401 attgacatta gatgaagtcc cacaaagaga aactgggatg acaccttatg aaatgatgtt
     8461 atcggaatca caagaacgga tgttgatttg tgttaaatca ggagaggaag aaaaaatcca
     8521 agaactattt cagaagtatg atctagatgc agtcactatc ggtaaagtaa cagacgacgg
     8581 gcaatatcgg ctatatcatc aaggagaaga agtagcaaat ctgccagtcg atgcattagc
     8641 tgaagatgca ccagtttatc ataaagaaat gaaagaacca gctcgaatcg tagaatttca
     8701 gcagttagct ccttatcagc cagttatcga agaacctgga caagtattgc ttgatctgct
     8761 gcagcaaccg acgattgctt caaaacgttc aatctacgaa acttatgatt cacaagtaca
     8821 aaccaataca gttgtccgtc caggaagcga tgctgctgtg atgagggtta gagggacgaa
     8881 taaagcgtta gctatgacca cagactgcaa cgcgcgctat ctgtatttgg atccaaaaat
     8941 cggaggacaa atagcagtgg cagaagctgc tagaaatatt attgctagcg gtggtaagcc
     9001 cttggcgatc accgactgcc tgaactatgg ttctcctgat aaaccagaag ttttttggga
     9061 attatcgact tcagctgacg gaattgctgc tgcgtgtgaa acgttaggaa ctcctgttat
     9121 ttcaggaaat gtctctttat ataatgaaac ggatggtcaa gcaatctatc caacaccaat
     9181 gattggtatg gtcggattga ttgaggatca taaacatatc actactcagg aattcaaaaa
     9241 atcaggggat ctaatctata tattaggtaa gacgtttgca gattttgatg gcagtgaatt
     9301 acaaaagatg cagcttggaa gaatcgaagg tgtgatccga aactttgatc tatccattga
     9361 aaagagaaac caagagttag tattgacagc catccaaaac ggattgattg aaagtgctca
     9421 tgactgttca gaaggtggct tggcaattgc actggcagaa tcagctttca aacatcagct
     9481 agggatatct gtacagtttg aattgtcctc tgctcaatta tttgcagaaa ctcaatcaag
     9541 atttgtgttg acagtcgcac cggaaaataa gacacgattc gaagaaatga tgggagatgc
     9601 tgctgtttta gcaggtaagg tgacggatga agcgataatc gaaatttcag caactgacgg
     9661 tcagatcaaa atagaaactg cagtagcaag aaagtgctgg gaggatgcaa tcgtatgtct
     9721 tatgaagtga agagtttaaa tgaagaatgc gggatcttcg gtgtttgggg tcatccagac
     9781 gccgctagtg tggcatactt cgggctccat agtcttcagc atcgaggaca agaaggtgca
     9841 ggtatcgtgt ccaatgatcg aggaaagctt aaggggcatc gagatttggg gctgctttct
     9901 gaggtattca aagatcaaag aaaattgcag cagttaacag gagaagctgc aatcggacat
     9961 gtacgttatg caacagcagg caatggaagt gtggacaata ttcagccttt tctattcaaa
    10021 tttcatgatc agtcgatcgg cttagcgcat aatggtaact tgatcaatgc tcgttccctt
    10081 agaaaagagt tagaggaaaa tggggcgatc ttccattcta actccgatac ggagatattg
    10141 atgcatttga tccgacgaag tgaagaaaca gattttttaa ataaattaaa aagtgctttg
    10201 aatgaagtca aaggtggctt tgcctattta ttaatgacag aaacggcaat gattgccgct
    10261 ttggacccaa atggttttcg tccattagca atcggacaaa tgaaaaacgg agcatacgtg
    10321 attgcctcgg aaacttgtgc attagaagtt attggggcac gttttataag agatgtccag
    10381 cctggtgaga tagtgatcat caatgacgaa ggaatccaga ttgattgctt tacgaaggac
    10441 acgcagcttt ctatttgttc gatggaatat atctattttg ccagaccaga ttcaaacatc
    10501 gcagggatca atgtgcatac tgcacggaaa aatatgggac ggaatttagc gaaagaatcg
    10561 ccagtaaaag cagatatggt cgttggcgta cctaattctt ctttgtcagc cgcaagcggt
    10621 tatgcggaag ctagtggaat cccttatgaa atcggattag taaaaaatca atacattgcc
    10681 cgaacattta tccagccaac gcctgagcta agagagcaag gtgttcgaat gaaattatct
    10741 gctgtacgag gagtagtgga aggtaaaaag gtgattatgg tcgatgattc aattgttcgg
    10801 ggtacgacaa gcagacggat cgtccatctt ttgaaagaag caggggcaaa agaagtccat
    10861 gtgaggattg cttctcctcc tttgaaatat ccatgttttt acgggattga tattcaaacg
    10921 cgtaaagaac tcattgcggc aaatcacacg attgatgaaa tcaaagaatg tatcggtgca
    10981 gattctttag gttttttaag tgaagagggt ctgatcgaat cgatcggtct ggaaaaagat
    11041 gctccgtatt caggattgtg tatggcttat ttcaacggag attatccgac gccgttatat
    11101 gactatgaag aaaaatatca ggaatcacta aaagaaaaag tatcgttctt ttaaaagcag
    11161 accaagaagg gagtatatga attcaatggc aaatgcatac gcaaaagctg gcgtagatgt
    11221 cgaagctgga tatgaagtag tagaacggat caagaagcat gtcaaaaaaa cggaacgttt
    11281 aggtatgatg ggcgctatag gaggattcgg cggttgtttt gatttatcgg tctttcaatt
    11341 aaaagagcca gtccttgtct cggggaccga tggcgtgggg acgaaattag cagtggcaat
    11401 cgaagcagac aaacacgata caatcgggat cgactgcgta gcgatgtgtg tcaatgatat
    11461 cttagcacaa ggcgcagagc cgttatattt cttagactac ttagcaacgg ggaaaaatga
    11521 tccgttacgt ttggaaaatg tagtaaaagg tgtagcagat ggatgtgttc aaggaaatat
    11581 ggcgctgatt ggcggagaaa cagctgaaat gcctggtatg tactcaggta atgattatga
    11641 tctagccgga tttgctgtag ggatagctga aaaatctcaa ttattgacag gagaaacgat
    11701 caacccagga gatgtgttgc ttggaatagc atctagcggg attcattcaa atgggtactc
    11761 tcttgttcgc aaaattttct ttgagcagca gcagttttct ttatcatcaa aactgccaga
    11821 attgaaaaaa acacttggcg aagaattgct tgagccaacg aaaatctatg tttcttcctt
    11881 gcttccgctg atcaaagaaa agaaaataaa aggagctgca catatcactg gtggagggtt
    11941 tgtagaaaat atcccgcgaa tgttaccaga atctatggct gcacaaatca aacttggctc
    12001 atggccgatt cttccaatat tcggctgttt acaaaaatat ggtgaaattc ctgagatgga
    12061 gatgtatgaa atcttcaata tgggcatcgg tatggtcctg gcagtccaag aatcgaaagt
    12121 aaaagaaata gtacacgaaa ttgagcaagc tggcgaaaaa gtttatgaaa tcgggaaagt
    12181 gatcgccaaa gaaaaacatc gtgtggtgtt tataggagaa gaacgatgag aatcgctgtt
    12241 tttgcttctg gaaacggaag caactttcaa gcgctagccg attatctatc gaaaaaagga
    12301 ttggaatctt cgattgactg gttgttttgt gatcagcctg aagcatatgt tttgaaacgt
    12361 gcaactgcgt tgagtgtacc ggcagattgt ttttcaccta aagaatttga ttcaaaaaaa
    12421 gagtatgaag aagcaatttt gcataaattg aaagaaaaaa agatcgattt gatcgtatta
    12481 gctggatata tgcgaatcat tggaccggta ttgctgaaaa attatgataa aagaatcatc
    12541 aatatccatc cgtctctttt acctgccttc cctggtttgc atggcatacg tgatgccttt
    12601 gaagctggag taaaagaaac gggtgtcaca attcattata tcgaccaagg tgtagatact
    12661 ggaccaatca tccggcagga aaaagtgaga attgaacagg aggacacgtt cgattcttta
    12721 gaagaaaaaa tccatagagt agagcatcga atctatccag aagtgattag tgaaattatt
    12781 gaaaatggag gctatctgaa atgacaagag cattgatcag tgtatcagat aaaactggga
    12841 ttgtagcatt cgcccaaggt ttgattgaac aaggagtaga aatcatttca acaggaggaa
    12901 caaagcatgc gttggataaa gctgggatca aaacgctttc gatcgaagaa gtgacagggt
    12961 ttcctgaaat gatggatggc cgtgtcaaaa cacttcatcc taaaatccat ggtggtcttt
    13021 taggaagacg cgatctccct agccatatgg aggcaatgga aacggaaaat atccaaccaa
    13081 tcgactttgt ttgtgtcaat ttatatcctt tcaaagaaac gattatgaaa ccagatgtcg
    13141 aaatagcaga agctattgaa aacattgata tcggtggtcc tagtatgctc cgtagtgcag
    13201 caaaaaacca tgaatttgtc acctctgttg ttgatccatc tgactatgaa gaagtacttt
    13261 cagaattacg tcagtatggg catacttcac tttctttcag acaaaaacta gcggcaaaag
    13321 tctttcgtca tactgcggca tatgatgcac tgatcgctca atacttaacg gattgggtgg
    13381 aagaaaaaga accggaaaaa ttgacactta cgtatgaacg aaaacaaacg cttcgttacg
    13441 gtgaaaatag ccatcaagcc gctgcctttt atcagtccgc tcttctggaa aaatattcga
    13501 tcgcagcagc aaaacaactc catgggaaag aactttctta taataatatc cgagatgcag
    13561 atgctgcgct acggatcatg aaggaatatg acgagccaac ggtagtcgcc ttgaaacaca
    13621 tgaatccttg tggtattggg acagcatcta cgattttaga agcgtgggaa gcagcgtatg
    13681 aagcagatcc tgtttcaatc tttggtggga tcatcgtttt gaatcgagaa gtagatctgc
    13741 caacagcaga acagatgcat aagctgtttc tagaaatcat cattgcccct tcttatacac
    13801 aagaagcttt ggaattactg caaaaaaaga aaaacatccg attactgaca gtagactttg
    13861 cgcaggaaga aaatcaaaat cgagaagaaa cggtctcagt cttaggtgga cttcttgtac
    13921 aagatcaaga tctggcaata gaaaatgaag aagagtggaa agtcgtgaca aaacgtaagc
    13981 cgactccagc agaagcaaaa gcattagcgt ttgcttggag agctgtcaaa catgtgaaat
    14041 ctaatgcgat cgtactggca aacgaacgtc agacagtagg aatcggagcc gggcagatga
    14101 atcgagtcgg ttcagtaaaa attgcgattg aacaagcgca agagaaaatg gaaggtgcag
    14161 ttttagctag cgatgcttat tttccaatgg atgattcggt tgaatatgca gccaaacatg
    14221 gtataaaagc aatcattcag ccaggaggaa gcatcaaaga ccaagattct attgatatgg
    14281 cgaataaata cgatctagca atggtcttta ctgatgttcg gcattttaga cattagagag
    14341 gagtcggata aatgaacatt ttggtgatcg gtgcaggtgg tcgtgaacat gcaataagta
    14401 aaaaactttt agccagtccg aaggtggata cggtgtattg tgcgccagga aatccgggga
    14461 tgacaaaaga tggtatcaat tgtgtcgcta tcagtgagcg agatcattca gcgttaatcc
    14521 attttgttaa gaaagaggct attgattgga gtatcgttgg tcctgaagtt cctttgttaa
    14581 atgggatcgt cgatgatttt atggcggaag gattgaaaat cttcggtccg acaaaagcag
    14641 cagctctgat cgaaggttcg aaaagttttg ctaaacaatt aatggatact tatcaaatcc
    14701 cgacagctaa atccagaagt tttgaatgtt acgaaaaagc agcagcgtat atcaaagaaa
    14761 atggtgcgcc aatcgtagtg aaagcggacg gcttagcagc tgggaaaggt gtagtcgtgg
    14821 ctaccacagt gaaggaagcg ctggatgcgg cagaacaaat gttgaaccag catcgatttg
    14881 gggatagtag caaaaaaatc gtcgtagaag agtttttggc aggagaagaa ttctcccttt
    14941 tggcgtttgt ccgtaatacc aaggtgtatc cgatggtcat ttcgcaagat cataagcggg
    15001 catttgataa cgataaaggg ccaaatacag gcggaatggg tgcttattca ccagtaccgc
    15061 aaatttcaaa aaagatgatt gaaaaagctg tggaagaagt tttaaaacca gcggctttgg
    15121 gaatgaaaga actaggacgc tctttcacgg gtgttttata tgcgggtttg attgctacga
    15181 aggaaggacc aaaagtgatc gaattcaatg cgcgtttcgg tgatcctgaa acgcaagtcg
    15241 ttctttctcg gttgaagagt gactttgcgc aagtaatcga tgatttatta gagaatcgta
    15301 cagtagagtt aaagtggcag caagaaggat acaatgtagg cgttgtggtt gcagctgccg
    15361 gttaccccga agaatatgag acaggaatgg ttctaccaga ttttctggaa gaagagcttt
    15421 ctgtttacta tgctggagtt tcctcaaata acggcaaact agttggagca ggaggacgtc
    15481 tttatttagt agaagcatac ggtgagacga tcaaagaagc acagaaaaaa atttacaccg
    15541 cgttaatgga taaaacgaca gatggaacat tttatcgtac agatattgga gaaaaagctg
    15601 ccaatagcga actgatcgtg aagtaaatca gctgcttcca tttataaaaa atacaacaaa
    15661 cataatcgga agcgccgatt ttagtgaagc cttttcataa agagtgtggt acacttttgt
    15721 tgtgtatata aataaggagg agttcagatg aaaaaaattc aattaggtac ttctgatcag
    15781 aaagcagctg cagtgatctt aggctgtatg cgtatcaaca atgcggaaga tccagtaaaa
    15841 gtgattgaga cagcttatga aaacggcatt gatttttttg accatgcaga tatttatgga
    15901 aacggtgaat gtgaagaaat ctttgcagat gctctagcaa aaacatcaat caaacgagaa
    15961 gatcttttta ttcaaacaaa atgtggaatc gttccaggtg tgatgtttga tttttctaaa
    16021 aaacatatca ttgaatctgt agaaaaaagt ttgacccgtc tgaaaatgga ttatgtggat
    16081 gctttacttc ttcatcgtcc agatacattg gtagaaccag aggaagtagc tgaagcgttt
    16141 gatgaattgg aacggtcagg aaaagttcgt tattttggcg tcagcaatca aaaaccaatg
    16201 caaatcgaat tgctgaaaaa atatgttcgt caaccattag tagctaatca gctgcaattt
    16261 ggcttgaaac atactggaat gatcgatcaa gggattcatg tcaatatgac agatgaagca
    16321 agtatcgatc atgacggtag tgtacttgat tattcccgat tgcatgatat gacgatccaa
    16381 gcatggtcac cataccagta tggatttttt gaaggtgtat ttatcgggaa cgaaaaattc
    16441 cctgaattaa accaaacatt agaagaacta gcagctaaat atcaaacgac accaactggc
    16501 ttggcttctg cttggatttt acgccatcca gccaatatgc aagtgatcgc cggtacgatg
    16561 aacgtagggc gaatcaagga aatcgctcaa gcttcagaaa ttaatatgtc tcgggaagat
    16621 tggtacgaat tgtaccgtgc tgctggaaac attttacctt aaagattgtc ataagcaggt
    16681 caaaagggct ataaattaaa aagacagaat aacttgaaag ctgttttcag cttcgagata
    16741 ttctgtcttt ttttattgca aatggaaaga gtatcattgt acggatttcg tttattagtt
    16801 atttttgttt ttaaggaaaa gctgtgtccg atattctttt ggagaaatcc cttgtttttt
    16861 cttaaataaa taactgaaat agttagggtc attataacca atttcaaaag caatatctga
    16921 aatacgatag tctgattgct gcaataattt ttttgctaat tcgattcttt gttctgtcag
    16981 ataatcgatg aatgtttggt taaacgattg agaaaaaatc gtactgaaat gagagggact
    17041 taatgcaact tgttgagcga cccaagttaa ggacatatct ggatcggtaa aattttcttt
    17101 gataaaatcc aatgcatggt tcaaaattgg ctgatatttg ttccttgaag gctgtgaggg
    17161 cagagcagtt aagaaagaaa tgaacatata aactgtcgaa cggtaaagcg agtcgtcggc
    17221 gctgattgct aagcgttgac tggtggatgt gcaatgaacc atttcttgcg ggattttctt
    17281 ttctttcagg cagacatgct ggagtagctt agtcaagaca aataaacgat ataatcgtgt
    17341 tcgagttggt gtgccttgtg gtttcgtcaa gtgttcgatg tacttagata cttcgttttt
    17401 cgataatgaa gacaaatcaa gtgagaggtc aaagtaaaag acagaatcaa ctggaagttt
    17461 cgcttcttga atcaaatcct catagctgat aatttgttct ttaagggaac tggcggtttc
    17521 aagtatcatc tgcgtgatct caaaagatgc aggtaattcg ctgattcttt ctgcgacagg
    17581 accaatggcg acagaaaaat tatttttttg tcccttttct aattcatgga ccaacgtttg
    17641 ggctacctga taactggtct cgatcacatt ttcttcaaga gaatcaacta ttaaaaattt
    17701 gatgaatcga gaggaaacag cagagaagat cacttgttga ttctccctaa ataaaaactt
    17761 caaataatca ctgagatgag cgtaatcttc aaagtctgta ctgacttggt ttgctgctaa
    17821 taatacggtc atttttttcc ctgctaaaga gcgattgaaa gattcagcaa gtttcaatgt
    17881 ttcactgatc gtaagtgagc ctttgaacaa accgttcaag aaatgatttt ttttcaattc
    17941 gaaaacaaga tcagaagaag tgtgcgtttc tttttgctgc aaatctaaag cagttactgc
    18001 tttttctatt gcttgtttca attcgctact tttaatcggt ttaagcagat aatcatctgc
    18061 ctgtacctgg attgctcctt tcatataatc gaaatcgtcg aatccactaa taaatatgag
    18121 acgagtccaa ggaagtagtt tccttgcttc ttttgaaaaa gtcaacccgt ccatgaaagg
    18181 catttggata tcagtcagaa tgatgtcggg ctgtaagtcg ataattgaag acagtgccat
    18241 ctcaccatct ccagcttcgc cgacataagt gattggataa taagaaccaa ggacagttat
    18301 ttgttcccgc aagttattgc gaatgaggta ttcgtcttcg atgataaaaa ctttatacat
    18361 gtttgtttcc tccaattgtt ttgggagata gagggatcat cagtgtgaca gttgttcctt
    18421 ctcggtattt gctgtcaatc gtgataggtt tttgatcatt tccgtaataa agaaggagac
    18481 gcttgtttac gttgtaaagt ccgtagccga tactgaaatc tgaatcgatc cctttggcta
    18541 actctttacg tatttctgcc agtttttctg cagggatgcc aatcccgttg tctataactt
    18601 tgaattggat catctcatca attttgcatg tggtaatacg aataaatcct ttgtgacgga
    18661 cgtacttcgt tccatggtat acagcatttt caatcaatgg ctgcaagatc atttttaaaa
    18721 tagaaacagg aaaaagtgtt tctggtatat cgatcttata gtcaagcata tggccgtatc
    18781 ttatttttaa gatgactaga taatcattga tatggctaat ttctttttca agaggaatcc
    18841 agtcttgtcc tttgcttaaa gacgtacgaa aaaagccgga aagtgcatgg gtcatagtca
    18901 gagcttcttc aaagcagtct tgttcgatca aagaaataat ggcatccaaa ctgttataaa
    18961 taaaatgggg agtaatctga gcttgcaata cacgaacttc actttgtgct aaatggtatt
    19021 gtttcaaagc gttctcccgt aaagactggt ttagatcttc cgccatttta ttcatgctag
    19081 cggtaagtgt ttgtaattcc agtgtttccg gcagggttaa acgaaagcca aggttccctg
    19141 aagaaagttg ttctgacatc tcgatcagct gattgatcgg ttcttggacc tttctctcta
    19201 aaaagcgacg attctttttc gcatagaaaa aaatggtact aagaactcca agttccacta
    19261 ttgttaatag aatgagcgag cggacaagtg tcgtattttt tttgcttgct aagtcgatct
    19321 ccacacgaac aaaatcttgt aagatatctg acagcagttg agtgacagaa tctacctgat
    19381 tgatgatctc ttcattggta tccataaaat cttttgccaa gatattattg atcagctcgt
    19441 tttgataatc ttccagtgta tcgatcgtac gtagcgatac atctaacgta ctaatttctt
    19501 taagtgtatt tgtattattt ttgctctctc gaatatcatt cctaagttct tctaggatat
    19561 tttcctcatt gaactcatct acggttactt ggccagaaat caagtcccac atactttcca
    19621 aaactttttg atcaaccttc aaagcaattg catctgcttt ttggatattt gcgatcgtct
    19681 gttgataaat caaaagctgt tgcgaatata acagtatgct cagtagcaaa gggataacag
    19741 caaatagaag catggctttg tttgcttggg caaaaatctg ttttatgctt aaattttttg
    19801 tcatgatact tctccagtca ataatttctg gttgggatgt tagctatatt ctgctgggaa
    19861 aagacagtct cggaaataaa aatatcttta ggtaatgttt ttcctgtttg aatatttcca
    19921 aaataacgag taacggtatt ggctacatac cagccgacat caggtttaca ctcgactaca
    19981 caattgacga ttcctgagcg tagcttctcg atcatatctt cttgtccatc aatagttaca
    20041 atgatcaaat cttctcccgg tttgattgat gtttctttta tcgcagctaa agcaccgtga
    20101 gtcatttcat cactatggct gtataataca tcgatttcgg ataaatcatt tgtttctaaa
    20161 tatcgtttca tctggtcttt tcctttcatc cgaataaaat caccgacaat tcggtgaaca
    20221 atagtcatcg gaggtttatg gtcgatggtt ccatcttttt ttagggttgg tttacgattg
    20281 attgattcca aaaatccttc ggagcgtagc ttggtcggtg tggagttctc tgaaccagca
    20341 agttctagga ttctgatttc ctgtttcttt gaatttgtga agtgattgct gacaaataaa
    20401 cccgcccgat ttccttctgc tttgaaactg gggccaatgt gggtgacgaa aagctcggga
    20461 tcattcgttt gaatatgtct gtcgacaagt atgacaggga tccctgctcg ctttgcttct
    20521 tttaatacag agtcccaccc ggattcttgc aaaggagtaa aaatgatcat atccacttta
    20581 taggcaatga acgtccgtat atcttggatt tgccgttcct gattcatgta gccatttcgg
    20641 tagagaactt ggtaatcttc tttttcaagc gcagtgataa ttgattcagt atgctttttc
    20701 cgccagctac tctccgttcc tgattgcgaa aaaccaacga ctagtgggtc aggtgcttcc
    20761 tttgtattag gtgtacaccc catcatttgg taagcgatta caacaagggc taaaaaaaat
    20821 aaaacacgtt tcactttaaa tcactccatc tggatttgtt agtactatct tatcagataa
    20881 aagggaaaat caaagtcaaa aggaatcaat cgaaaaaaat ccaagaaact taaaaaaatc
    20941 caagacaaaa aaagtacacc taaaaaattt aaagtaaatc ccataaaaaa atcaatgtta
    21001 acgctttctt tattagctat actcggtgta tcaattgaaa tgggggataa aaaatgaaaa
    21061 acaaatttag aaagattgtt gggactatcg ctattttgac cgcaagcatt ctattaagtg
    21121 cgtgtggcaa ttcaggatct gcagatgata gtacggggta tgtaggaatc gctatgccga
    21181 ccaaatcagc agaaagatgg atcgctgatg ggaacaacat ggtctcggaa ttagaaaaac
    21241 tgggttataa aacagatctt caatatggag aagataaagt agaaaatcaa gtagcacaaa
    21301 tagaaaatat gattacaaaa ggggtagata cacttgtgat tgcttctatc gatggttcgg
    21361 ctttaactga tgtgttagct aaagcaaaag aagcagatat caaggtgatc gcctatgatc
    21421 gtttactgat gaattcagaa aatgtcgatt attacgcgac ttttgataat tttggagtag
    21481 gtgtatcgca agcagcttat attgaagagc acttaggatt gaaagaagga aaaggaccat
    21541 tcacgattga attatttggt ggctcgcctg acgacaacaa cgcactaatc aattacaacg
    21601 gagtgatgtc tgttttacaa ccatatatgg acaacggtca gttggtcgtg ccatctggac
    21661 agacgagttt cagtcaaatc gctacgcttc gctgggatgg ttctacggcg caagcaagga
    21721 tggataatct gttaagcgca aattatactg accaaacatt ggatgctgtt ttgtcgccat
    21781 atgatccaat aagtttagga atcatttcgt cattaaaagg cgtggggtat gggagtgaaa
    21841 gtaaaccttt acctgtgatt actggacaag atgcgacagt tgctggcgtt aaatcgatca
    21901 ttgccgggga acaaacacaa acgatcttta aagacacacg tatcttagca aaaaatacga
    21961 ttgaaatgat caaagcaatc agtgatggag aagaggttcc ggtcaatgat actgaaactt
    22021 atgataatgg ggtgaaaact gtccctacgt atttagcaaa tacagtatct gtcgataaag
    22081 ataattatca agcagagctg atcgacaccg attattataa agaatccgat ctgaaaaatt
    22141 aagaggaacc aaacaaggaa ctaaagagat ctaagtcaga gagaggataa gaattatggc
    22201 ggattgtatt ttaaaaatgg cagatatcac caaaaaattt tctggaatcc gagcgttaga
    22261 ttgcgtgaat ctgatgattg aacgtgggga aatccatgca ttgtgcggag aaaacggagc
    22321 gggaaaatca acattgatga atgtcttgag cggcttgtat ccatatggct cctatgacgg
    22381 ggaaatcttt tatgatggaa agctgtgtca atttaaagat ttgaaagata gtgaagaaaa
    22441 aggcatcgtc atcattcatc aagaattagc actaagtcct tatctttcaa taaaagagaa
    22501 tatttttctc ggaaatgaac aagcagaaag aggcgtgatt gattgggaac tgacagaaaa
    22561 gaaaacatat gaactgctga ataaagtcgg tttgtctatc gacccgaatc ttctagtatc
    22621 ccagattggc gtagggcaac agcaactagt tgaaatagct aaagcattat caaaatccgt
    22681 ccgtctgttg attttggatg agcctaccgc tgcattgaat gaagaagaaa gcgcgaattt
    22741 gctgagattg atccgagagt tgaaagagca aggtgtaacc tccatcatta tttcacataa
    22801 gctgaatgaa atcgtggata tcgcagatcg aatcaccatt ttacgggatg gacagacgat
    22861 tgagacacta gaaaatgaaa tgatcagcga agagcatatt attcgcggaa tggtgggcag
    22921 agatttagtt gatcgttatc ctgaaagaaa accggatatt ggagaagttt attttaaagt
    22981 agaagactgg acggtctatc accctcttga tcacgagcga atcataaacg accacatcaa
    23041 cttgcatttg aaaaaaggag agattgtagg aatagctgga ctgatgggag cgggcagaac
    23101 tgaattcgct atgagtctat ttggacattc ttatggaagc aagatatccg gacgtatatt
    23161 aaaggaaggg gcagagatat cagttaagac cgttccttca gccattgcaa acggactcgc
    23221 ttatgtctcg gaggaccgaa aatctttagg actgaatctg ctgatggata ttcgggaaaa
    23281 cgtgacgctg agcagtttgg ataaaataag tcaaaaagga gtattagata aagaaaaaga
    23341 agtaactgag gcagaaaatt tccggaaaaa gatgcgtata aaaacaaact ccatccatca
    23401 aattgtcagt agtcttagtg gtgggaatca acaaaaagtc gttttatcca agtggctgat
    23461 gacacaaccc gatattcttt ttttggatga gccaacccgt gggatagatg ttggggcaaa
    23521 atatgagatc tacacgatta tcgaagaaat ggcagcaaat ggcaaagctg tctgtcttat
    23581 ctcttctgaa cttcctgaaa tattagggat gtgtgatcga atctatacga tgaacgaagg
    23641 acgtattaca ggagaagtct cgcgagaaga tgcagatcag gaagtcttga tgaaattaat
    23701 gacgaaagaa gaggtgggtt aagttggaac aggcaaaaaa tcttacggat aaaaaagaga
    23761 aaaaactaaa tttgaaaagt agagcagctg atctttttgg tcgctacagc atggtgatca
    23821 ttttagcggg actacttatt gcgtttcaat tgatgacgga tggtattttc tggcgtccat
    23881 tgaatataac taaccttgtt ctgcaaaata gccatatatt agtactagct gcagggatgt
    23941 tattagttgt gctcttaggt catgtggatt tgtcggtagg gtcagtgatg gcgtttgttg
    24001 gtgcaattgc gggcatgatg atggtcaatc gccaaatgaa cccgtggatg acagttatcg
    24061 catgtcttgc agtaggcggg ctgatcggcg catggcaagg tttttgggta gcttatgcag
    24121 ggataccggc gtttatcgtg actttagcag gattgctgat gtttcgtgga ctgacacaag
    24181 ttgttctagg aggccaatct ctagcacctt ttccagctgt tttccaaaaa atgtctacgg
    24241 gttatttacc agacttaagc gaaggaagca gccttcactt cttgacattg attttaggaa
    24301 tgatcatcac gatcgtgtta gttgttggtc aatggcgaac aagaaatcgt cggaaaaatc
    24361 atttatttga agtggaatct ccaacagctt tcttggtaaa ggcggtattt acagcagtcg
    24421 ttgtactagg aataacttat atctttgcct cttatcaagg gtatccgatt attttgatca
    24481 ttcttggttt gatcgttgca gcatatgggt ttttgacaaa taaaacagta gcaggtcgac
    24541 agatttatgc gacgggcgga aatcgaaaag cggctgaact atcagggatc aaaacaaagc
    24601 gaatcacgtt ctgggtattc gtcaacatgg gagtcatggc agctttagct ggattagttt
    24661 tagctgctcg attgaatgcg gcaacacctc aagcaggtac atcgatggaa ctggatgcaa
    24721 tggcggcagt atattttggt ggtgcttcga cttccggcgg cattggaaca atcatgggtg
    24781 cgattgtcgg tggcttggtc atgggtgtat tgaacaatgg catgtcgatt ttaggcgtcg
    24841 gggtagactg gcagcaggca atcaaaggat tgatcttgct tttagccgtt gtgctagact
    24901 tgtataataa gaaaaaacga acaacgtagg agaacgccgg ttattcagaa aaaagactgt
    24961 gacaagacat ttgtcacagt ctttttttcg ctaaaagcga acattcattg tttcgtttgt
    25021 atagactaaa aataatatat gtttttttac cattcgtata aattctcttt ttgctcttga
    25081 tttttttgct taattgagtt aaattacata gggcgctaaa aaaagccttg attaaattaa
    25141 taatgttcgt gttttgctgg atgtgaacat caggaggata atgtaatgaa agtatttgat
    25201 tacgaagata ttcaattaat tcctaataaa tgtatcgtaa atagccgttc tgaatgtgat
    25261 acaacggtca cattaggtaa acatacattc aaaatgccag tagtgcctgc caacatgcag
    25321 acgattattg atgaaacaat cgcagaattc ttagcggaaa atggttattt ctatatcatg
    25381 catcggtttg atgaagcagc tcgaattccg tttatcaaaa aaatgaaaaa gcgtggattg
    25441 attacctcga tcagtgttgg cgtcaagaaa gaggaatatt cctttataga gaagttggca
    25501 gaagaatcgc taaatcctga ttatatcact atcgacatcg cgcatggaca tgctaactca
    25561 gttattgata tgattcagca tattaaaaaa tatttgcctg aaacttttgt gattgccggt
    25621 aatgttggta caccggaagc cgtccgtgaa ttggaaaatg cgggcgcaga tgcaacaaaa
    25681 gtcgggattg gtccaggaaa agtatgtatt acaaaaatca agacaggctt tgggactgga
    25741 ggctggcaac tggcagctct tagatggtgt gcgaaagcag ctcgcaagcc gatcatcgct
    25801 gacggtggga tcagaacaca tggcgatatt gcaaaatctg tccgttttgg tgcgacaatg
    25861 gtcatgattg gttcattgtt tgccggtcac gaggaatctc ctggcgaaac aaaagtcgaa
    25921 aatggaatcg tgtataaaga atactttggt tctgcttcag aattccaaaa aggcgaaaaa
    25981 cgaaatgttg aaggaaagaa aatctggatc caacacaaag gatctttgaa agatacacta
    26041 gtggaaatgc aacaagacct tcaatcatct atttcttatg ctggaggaag agatctggaa
    26101 gccattcgaa aagttgacta tgtcatcgtg aaaaattcga tttttaatgg agacacaatc
    26161 taaaaaataa ataaaatatt aggatgttgt gaaaaagttg tcctgcatta agtaataaga
    26221 acagccaaac aaaaatagtt ctttatatgt tttgtttggc tggaattatt atcgcagata
    26281 caagcttttg aacatcatta ttcagaataa gggactgtga taagattttt gtcacagtcc
    26341 cttatgtttt gcaaaaaaag tttttctttt ttctcgtaaa ttcggtaaaa tcaaaagaaa
    26401 aaatagacaa tagaaaagag aagcttatgt gggaaaaagt agagaaaaaa caaggaccga
    26461 tttatcgtca aattatggaa cagattatga gagaaatcga aaacggaagg atcaatcctg
    26521 gaagtcaact tgaatcagaa cgaaaactag ggcaactttt tggtgtgaat cgttccaccg
    26581 ttgtccatgc attggaagag cttagggagc ttggggtact aacgagcaaa cgtggcagcg
    26641 gatggtttgt taatcagact gaatggggga aattcgctgt tccaagaatc gattggcggc
    26701 aaatgatctc tcctcgttat gagcagacgg actggtatga gcaaaaaata agagaagcaa
    26761 agcagatcca aaaaacatcg tttcttgatt tatacgctag cgagatgcca gatgagcttt
    26821 tgccgaattt tgagtttcct tcttattctc ttgaagaaat cttagcggaa gaaaaagaaa
    26881 tggatctttt aggctatact ccgttacgta acaaaatcaa acggtatttg gaaagtaaat
    26941 ttgaattggc attttccccc aaccagcttc ttgtgactgg tggcggtcag caagcaatct
    27001 ttttgatttt acaaacgatt ctttcagctg gagaggggat agctgtagag tcaccttctt
    27061 ttttttaccg attacctctt ttcaaagctt caggaacgcg tttatttggg ataccgatgg
    27121 atgaagaagg gatcgactta gaaaaactga aagaatcgat ccataaaaat aaattgaaag
    27181 cagtgcttat caacccgaat tttcaaaacc ctacaggaaa agtgatgagt caaaaacgac
    27241 gagaaaagct agtagaattg tgtcgacagt atcggcttcc aattattgaa gacgatgtat
    27301 ttacggatct ttcatttcta aaccaagaag aagtccttcc tatacgaacg attgatccgg
    27361 ataatgtgtt atacgtcggt tcgttatcta gggttttagg aaaaacaacg aaaatcggtt
    27421 gggtgatagg acctgagact tttatctcgc aactagctca agcgcaagaa atgatggagt
    27481 tttcgatgaa tatccttaca caaatcacag tggctaatgt atttaatgaa gcaattgatt
    27541 tgaaaatgca agatgtaaga aaaaagttga aagaaaaaag ccgactgata agcgcttggg
    27601 gagattcaca atcgtttttc aagcttcatt caattttagg tggttactat gcctggatta
    27661 cttgggatgg gaaaaaacta gaccccgaac tagcagaaaa agtagtagat atgggtcttg
    27721 gaattgcacc aagtctactt tttgggcaag atataaatgg aatcagaatc aattacagta
    27781 ggctggatga aaaccaaata ccgttgtttt attcaaagat gaaaattcta gaaaagtggt
    27841 taacagaata atttcacaaa gtaaatagat taaagaatat aacagcttgg atttaaattt
    27901 caagctgttt cttttttttc ggaaaaagaa tacgatataa agaaactaag aaaaaaccat
    27961 tatttgtacg tccgttcacg atgtgtaaat aaaataaatg tttgcaacaa aactgtataa
    28021 caccaacgtt tttaaataga aacagatggt tgtaagtttg attagaaaat agaaaaaatg
    28081 cagatgaaat ataaaacgct ttccttattg tgattttatt cactaatgct gttaatatgc
    28141 ggattggcat aatttccttt catcgtgaaa atttgtgctt ttagcttgta tttctcataa
    28201 atccatgata atttattaat gaagacggtt aaatagtgat acagttttat tcactgtttc
    28261 aatcgcagaa tgaagagatt ttcttataga tgagagagga atgtgtgaca aatggcaaac
    28321 aaaaaagcac cgatcgactt ccaagcactg ctagatgaag tcaatgcaga tttcccagta
    28381 tatcaagcac ttgatcaaga tggaaacgta gttaacaaag atttagtacc tgatttatcc
    28441 gatgatgaac tcgttgaatt aatgactcat atggtatggt ctcgtgtatt agaccaacgt
    28501 tcaacagcgc tgaaccgcca aggacgccta ggcttttttg caccaactgc tggacaagag
    28561 gcaagtcagc tggctagtgc ttacgcattt gataaagaag atgttcttct tccaggttat
    28621 cgggatgtac ctcaactagt aaaacatggt ttacctttat ctcaagcttt tttatggtct
    28681 cgaggacatg ctgcaggtaa ttcttatccg gaagaattga aagctctacc accacaaatt
    28741 atcatcggtg cacaatatgt acaagcagcc ggtgttgcat taggtttgaa gaaacgaaac
    28801 aagaaaaatg tcgtcttcac ttatactggt gatggcggtt catcacaagg tgatttttac
    28861 gaagcaatca atttcgctgg cgcttaccat gcaaatgccg tattttatat ccaaaacaac
    28921 gggtatgcga tctctacacc tcgtgaaaaa caaactgcag caaaaacact tgcacaaaaa
    28981 gctgtcgcag ctgggattcc tggtattcaa gtcgatggaa tggatccgct tgcagtatat
    29041 actgtatcta aaatggcacg ggaatgggcg atcagtggaa atggaccagt cctaatcgaa
    29101 acactgactt atcgttatgg accacataca ctatctggtg acgatccaac ccgctaccgt
    29161 acaaaagaca cagatgacga ttggcagaaa aaagatccat tgatccgctt ccgtaaatat
    29221 ttgacggaaa aaggactttg gtctgaagaa aaagaagaac aagtcatcga agcaactaaa
    29281 gaagaaatca aagcagctat tgctgaagcc gacaaagtac caaaacaaaa ggtttcagat
    29341 ttcttgaaaa acatgttcga agtatctcca caatcaatca aagaacaaat tgcaatttat
    29401 gaagcgaagg agtcgaaata atcatggcac aaaaaacaat gatccaagca atcacagatg
    29461 ccttagcact tgaactagaa aatgacgaga atgtcgttgt ttttggtgaa gatgttggga
    29521 aaaacggagg agttttccgg gcaactgaag gattgcagga aaagttcgga gaagaccgag
    29581 tattcgatac tcctttagct gaatctggaa tcgcaggatt gagtttcggt cttgcgttag
    29641 aaggatttcg tccagttcct gaaatccaat tctttggttt tattttcgaa gcaatggacg
    29701 aagtagtagc tcaaatggct cgtacaagat atcgaatgag cggcactaga aatctaccca
    29761 tcacaattcg ttcgccattt ggtggcggtg ttcatacacc agaattgcat tcggataatt
    29821 tagaaggttt gatcgctcag tctcctggta ttcgagtagt gattccttcc aacccttatg
    29881 atgcaaaagg actattgatt tcttctatta gaagtaacga tccggttgtt ttcctagaac
    29941 acatgaaact atatcgctca ttccgagaag aagtgccgga tgaagcttac gaagttcctt
    30001 tggataaagc agcagttact cgtgagggaa cagatgtatc tattatcaca tacggagcaa
    30061 tggtgcgtga agcaatcaaa gcagcagaca atcttgcaaa agaaaatata tctgttgaaa
    30121 tcattgatct tcgtacagtt gcgccattag atgtagaaac gatcattcaa tccgtggaaa
    30181 aaactggtcg cgtagttgtt gtccaagaag cacaacgaca agcgggcgtt gccgcgcaag
    30241 tagtttctga gatctcagaa cgcgctatcc tttcattaga agccccaatc ggacgagttt
    30301 ctgcacctga tacagtcttc ccatttggac aagcagaaaa tgtctggttg ccaaatgcaa
    30361 aagatatcga agacaaagta aaagaaatcg cggaatttta agaaaagaag ggacgaaaca
    30421 aagtcatggc ttatcaattt aaatt
//
LOCUS       DBNICI010000014        30118 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.014, whole genome shotgun sequence.
ACCESSION   DBNICI010000014 DBNICI010000000
VERSION     DBNICI010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 30118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.014"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(197..2095)
                     /locus_tag="AC1ZCC_000526"
     CDS             complement(197..2095)
                     /locus_tag="AC1ZCC_000526"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HIQ1628805.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(2256..3518)
                     /locus_tag="AC1ZCC_000527"
     CDS             complement(2256..3518)
                     /locus_tag="AC1ZCC_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1628806.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(3511..4410)
                     /locus_tag="AC1ZCC_000528"
     CDS             complement(3511..4410)
                     /locus_tag="AC1ZCC_000528"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1628807.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(4555..6333)
                     /gene="uvrC"
                     /locus_tag="AC1ZCC_000529"
     CDS             complement(4555..6333)
                     /gene="uvrC"
                     /locus_tag="AC1ZCC_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIQ1628808.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(6515..6829)
                     /gene="trxA"
                     /locus_tag="AC1ZCC_000530"
     CDS             complement(6515..6829)
                     /gene="trxA"
                     /locus_tag="AC1ZCC_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIQ1628809.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(6916..9276)
                     /locus_tag="AC1ZCC_000531"
     CDS             complement(6916..9276)
                     /locus_tag="AC1ZCC_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0045910 - negative regulation of DNA
                     recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HIQ1628810.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(9391..9939)
                     /locus_tag="AC1ZCC_000532"
     CDS             complement(9391..9939)
                     /locus_tag="AC1ZCC_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HIQ1628811.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(9998..10435)
                     /gene="zapA"
                     /locus_tag="AC1ZCC_000533"
     CDS             complement(9998..10435)
                     /gene="zapA"
                     /locus_tag="AC1ZCC_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HIQ1628812.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            10637..11554
                     /gene="rnhC"
                     /locus_tag="AC1ZCC_000534"
     CDS             10637..11554
                     /gene="rnhC"
                     /locus_tag="AC1ZCC_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HIQ1628813.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(11642..12553)
                     /locus_tag="AC1ZCC_000535"
     CDS             complement(11642..12553)
                     /locus_tag="AC1ZCC_000535"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIQ1628814.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(12954..13532)
                     /locus_tag="AC1ZCC_000536"
     CDS             complement(12954..13532)
                     /locus_tag="AC1ZCC_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIQ1628815.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(13552..14220)
                     /locus_tag="AC1ZCC_000537"
     CDS             complement(13552..14220)
                     /locus_tag="AC1ZCC_000537"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1628816.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(14235..15305)
                     /locus_tag="AC1ZCC_000538"
     CDS             complement(14235..15305)
                     /locus_tag="AC1ZCC_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1628817.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(15476..16840)
                     /locus_tag="AC1ZCC_000539"
     CDS             complement(15476..16840)
                     /locus_tag="AC1ZCC_000539"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIQ1628818.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(16837..18396)
                     /locus_tag="AC1ZCC_000540"
     CDS             complement(16837..18396)
                     /locus_tag="AC1ZCC_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1628819.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(18452..20932)
                     /locus_tag="AC1ZCC_000541"
     CDS             complement(18452..20932)
                     /locus_tag="AC1ZCC_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628820.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            complement(20916..21251)
                     /locus_tag="AC1ZCC_000542"
     CDS             complement(20916..21251)
                     /locus_tag="AC1ZCC_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628821.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(21265..22299)
                     /locus_tag="AC1ZCC_000543"
     CDS             complement(21265..22299)
                     /locus_tag="AC1ZCC_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HIQ1628822.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(22389..23069)
                     /locus_tag="AC1ZCC_000544"
     CDS             complement(22389..23069)
                     /locus_tag="AC1ZCC_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HIQ1628823.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(23353..24456)
                     /locus_tag="AC1ZCC_000545"
     CDS             complement(23353..24456)
                     /locus_tag="AC1ZCC_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HIQ1628824.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(24678..25148)
                     /locus_tag="AC1ZCC_000546"
     CDS             complement(24678..25148)
                     /locus_tag="AC1ZCC_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HIQ1628825.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            25481..27121
                     /locus_tag="AC1ZCC_000547"
     CDS             25481..27121
                     /locus_tag="AC1ZCC_000547"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HIQ1628826.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            27275..28597
                     /locus_tag="AC1ZCC_000548"
     CDS             27275..28597
                     /locus_tag="AC1ZCC_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HIQ1628827.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(28659..29090)
                     /locus_tag="AC1ZCC_000549"
     CDS             complement(28659..29090)
                     /locus_tag="AC1ZCC_000549"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1628828.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(29154..29990)
                     /locus_tag="AC1ZCC_000550"
     CDS             complement(29154..29990)
                     /locus_tag="AC1ZCC_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIQ1628829.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         8669 a         6251 c         5215 g         9983 t
ORIGIN      
        1 ccaagaggtc gtgagcctgc cgttcaactc tgagtcacaa ggagcaattt caaaaaacag
       61 cttttcatgt ttgttgaata gtgtgacttg tgaccgagga gttggcagaa gaacaccgtt
      121 tatccggaaa aagagactgc gacacgactt ttgtctcagt ctccagtcta tggaacaatg
      181 ttcactggaa ttcttctcaa tcgtcctttc gatagtccaa cagccttttg agctgggtaa
      241 tctgctgctg cagttccata ccttttgttg tatcacgatt gagtttgcgt ggtagttgct
      301 gttcgattac ttttttcaca tttgtttggt cggatttttt tgcaaattgt tgttcaatag
      361 gcaaaaacgg ataatgtgtc acgatttgga aaccataaga attgcataat agtgaataac
      421 cggcaattcc tgtcgttttt tgataagctt tggataaacc tccgtcaatg acaaatactt
      481 ttccatttgc tttgatcggt gactcccctt tttttacttt tactggcgtg tgcccattaa
      541 tgattgctga cccttcttct tttgtgtcga attctgctaa aattttttca ctgaaccact
      601 cttcatctcg caagtgataa taaggattgc ttttctcgac gtgcgtatga ggctctgtga
      661 gaaaataacg ttcaaaagtc gccattttac tcttgccgaa taaaggcgaa aatggcccac
      721 tccacgcata ccaaatcaaa tcggttgcta aatcttcttt tgcagtcaga tcttttgcgc
      781 tttcagttat ctgtttttca aaaaagccca gcagttcttt tccgcgataa gattggtcta
      841 attcaaatgc taagagctgt cccttctcgt ctacaggtat acagccatga aacagcacat
      901 gctgattgtt cgtgagatac attccccctt tttcgatcaa aaacgtcata tgtttttgca
      961 ttctcaaaga gtgctggaat gaatacatca aactatcaat cactttttgt tcctcttcca
     1021 agaatttttt tggatcatct ggctggatcg tttgaaaaca agttcctttc aactcataga
     1081 gctgaccttc taaatcaatc gttgatgctt catagtcgat tttttccagc aatttacgat
     1141 cgtccatttt aaattcaggt cgacgatcca ataactgatg ttctgatttg aattgaagga
     1201 tagaaagtgc ctgatggatt ttttctaatt cttcttgctc tctttcggat agatttttac
     1261 tgtttttagg cgcaaatact ggattcttct gataagtttg atgagcaaaa gaaaacaatg
     1321 gacgtaaatt caacccatat tctttctcta attcaaataa ataattatag cgtgctgcaa
     1381 tccgtaaaag tgtcagtaaa catgcctcag agccgcaata cgcaccaatc cacaaaatat
     1441 catgatttcc ccattggata tctacagaag aaaaatccat gattcgatcc atcactttgg
     1501 ctgcttgagt tcctcggtca aaaatatctc caacgatatg caaatgatcg atgacagatt
     1561 tttggatcgt ctgtgcaagt ttcgttaaaa atttttcttc ttccttcatc aaaacaagtt
     1621 gttcaatgat tttttttaca tagtcttttt ttccattgat acggtcatcg atatacaaga
     1681 gttcctcaat gatatagcta tattctttcg gcaatccttt tcgaactttt gaccgggtat
     1741 atttgactgt cgtaaaccgt aaaagatcga tcaaatgatg gatcagctgc gatttatctt
     1801 gttctttgta ccaatcagtc gttaatgcat attctggata agccgtaaaa atagtgaatt
     1861 ggctgatatc cgtttccgtc atttgttcag aaaaaaggtc tttgatcttt tctttaagat
     1921 ttcctgaacc gatacgcaga atatgatcaa aagcaggaaa ttcgccatgt atatcactca
     1981 aaaataactc tgttcctttt ggaagctgca aaattgcttc gagattgatg atttccgtga
     2041 tgacatcctc cgttgagggg aatttttctg aaagtaaatc taaataacgg tccattaaaa
     2101 cgcctccaaa aattggtata ctcaatatta taaagttgtt atacactatt ttcaatgaat
     2161 atatatccaa tatagtaaaa taaataaaat aggtctatac aaaaagaagc tgggaacaat
     2221 agatgtccag cttccttctg tattttagga ttgttttatg ctttttttcg ttcattcctc
     2281 ataattgaaa tcgattgttt caatgaactc cataatccgc cttcgctata aacgagtaca
     2341 ttcgatttgt acatgtttgc agacaagatc atcaataaga atgttacggc tacaagtacc
     2401 aacaaggaaa tcaatgctcc acccataccg actgtctcat ttgctaagcg aaccggcata
     2461 atatatgaag ataagaatgg aatatacgaa gtgatccgaa taacgatatt gtttggatct
     2521 gaagctccta gtatcaatcc aagcatgtat ccgcctagag atagataagt cactggcaaa
     2581 attgcttttg ctgtatcttc tgctttgttg accaaagagc cacataaagc tgctaaaaca
     2641 gaaaaaatca aaatgccaaa gatcatgaat agaagagaat accacaagaa tggtccaatc
     2701 aagttatcca atgagatacc gtctaatata tttttgacca tttccatatc tttgaattga
     2761 ctgtatccta taccaaaaat aataccgtat aatgccattt gcgttaatgc aacaagtaaa
     2821 acacctgtta attttccgta aaaatgcttt tgtgcagtcg tactggacag gatcacttcc
     2881 atgatccgcg tgcctttttc agaagcgatc tcttgtgcaa tgatctgcgc atacgtcaaa
     2941 ataataatga acaagacaat cgttgctaca taacttacga cgtattggac tgtcgagtta
     3001 tcttctccga ctgtcatttt tccttttgaa tcaaaattga ctttttgtcg agtcaaactt
     3061 gcttgttggc tcaagctagc tacttcttct ggtgaaatgc ctaattgact cgctcgaagc
     3121 atcgattgca agccagtcag ttgttgttgt atcagtagtt gtgtactttg tcctaatgaa
     3181 ttttcgctat aaagcatccc atttatctta tcattctcag ttgagactac taaatacgca
     3241 tcgatttttt cttccgaaag ctctttttgt gctttttctt cagaatcaac tgctttgaac
     3301 tcaaaatcat ctgttttgac ttggctcatt tgctgactga tttgctcatt atcagccaat
     3361 acgccgattt tattgacatc actattttgt tgagcaaagt ttcctgctac ataaatgata
     3421 cccagcatga taaaagggac taatatcatg atcaagaaag aaatcgactt aacatttttt
     3481 aaatagacat ctttggtaat aatccaaaac ttacgcatcg atttcacctg ctttcaattt
     3541 gaaaatctct tccaaagttg gcggttgctg gttaaacata ggaatgtagc cgaactgagt
     3601 cgcacgagta aagatttctt tcccagcttc aggatcttct aaggtaatct ctaacgtatt
     3661 gtcttcatga aggatcactt ttttcacgcc ttcgatttct tctacttcct gttggcttaa
     3721 accagattcc aagaataact tcgtacggcc aaatgattca cgaatctcat gtacttttcc
     3781 attcaaaacc atttttccgt ttcgcaacat gatcaaatga tcgcagattt tttctacatt
     3841 atccatattg tggctagaaa aaatgacaca tgaaccatgt tttttcaatt ccaatattcc
     3901 atcttttagc aattcagcat ttaccggatc taacccgctg aatggctcat ccaaaatgat
     3961 caatttaggt tcatggatta acgtggcgat cagctggact ttttgctgat ttcctttaga
     4021 caaagatttt actttatccg tctttttccc ttttacctga aatttttcca tccaaaagtc
     4081 gatttttggt tcaatctctt ttcttgattt ccctctaagt tctgcaaaat aaagcagttg
     4141 gtcttgaatc gttactttcg gataaagtcc ccgttcctcc ggaagataac cgatgacatc
     4201 ataatctttc tccccaagat gatggccatt ccataacact tctccttggt cttgtgtcaa
     4261 aaaatccaag atcaaacgaa aagtcgttgt cttcccagcc ccgttttggc caattagccc
     4321 catgattttt ccatcgggga tttggaaaga gacatgatcg acagctgtgt agttcccaaa
     4381 agttttaaca agatccttga cttctaacat ttcttcactc cttcttcaag aaatcacata
     4441 tatccttaga caccagacgt aagcgattta ttatttacca acaaaacgta aacgctcttt
     4501 ttgttgtaca taaaatgata ggacacttta ctttctttgt aaagtgccct ttcctcattc
     4561 ctgatgtaat ttatcaaata cgttttgtgc gacatttttc ggcagtccga tttcttgtaa
     4621 ttcttctgtc gtggctgctg tgatattttt caacgatttg aactctttaa gcagcatttt
     4681 ttttcgtttt ggtcctaacc cttcaatttc atctaatcgg gaagcaaaac tgtttttact
     4741 gcgtagctgc cgatggaaag tgattgcgaa tcgatggact tcatcttgga ttcgctggag
     4801 taagaaaaac tctggtgaat ttcgctcaag cggtactaca gccaaatctg taccgaatag
     4861 caactcattt gttttatgtt tatcgttttt tgccatgcca gcaataggaa tatcgattcc
     4921 taattgattt tctaagacat cttttgccac atctacttgt ccttttcctc catcgatcaa
     4981 gatcaaatca ggtaaaggca acccttcgcg aatcactcgc gaatatctgc gatagatgac
     5041 ttctctcatt gatgcataat catccggtcc gttgactgtt tttattttat atttccgata
     5101 ttcttttctt gccggttttc catcaatgaa tacgaccatc gcagccacag gattcgtccc
     5161 catgatatta gagttatcaa aagactcgat acgtattggg gtcgggatat tcatcgcgtt
     5221 tcctaaacgt tcaactgctc cgatcgtccg ttcctgtttt ctggcaatca gatcaaaccg
     5281 ttcattcaaa gcaactgagg cattcttact cgccaattca actaattttt tcttttctcc
     5341 tcgttttggt tgcagcactt tcgtagctaa caaagcttct acactgggct tgtcgatcgt
     5401 atcaggaatc aagacttctt taggaatgaa atgctcattt tcctgataaa actgaccgag
     5461 gtaagtcaag aaatcttctt ctgcttcgtt atagaaagga aacattgaaa catctcgttc
     5521 aattagcttt ccttgtctta cgaaaaacac ttggacacac atccatcctt tatcaatcgc
     5581 ataaccaaat acatctcgat ccagtaaatc agtatttgtc attttttggc gagtcatgat
     5641 tgtctcaatg gcacggatct ggtctcggta ttctgcagct ttttcaaact ccatgttttc
     5701 agcagctttt tccattttct gatggatctc tgactcgatt ttttcgtgtc ctccgtttaa
     5761 aaagtttttc acttcatcaa cgattttcgt atagactttc ggatctacgt cgaaataata
     5821 aggacacaga cattgtccca aatgatagta aagacaaggc tccttcgtct gactaggttt
     5881 gcattttctc agaggaaata atcgatcgag gatcttcttt gtctcattcg ccgcaccgac
     5941 atcgggatat ggtccaaaat acagtgcttt gtcttttagt accttacgag taatcaccaa
     6001 acggggatat ttttcattcg ttatcttaag gaaaggatag gttttatcat cctttaacat
     6061 aatgttgtat ttaggatcat ttttctttat cagattgatc tctaagagca atgcttcgat
     6121 attcgattct gtaacgatgt attcaaagtc ttcgatttca ctaacaagcc gttccgtttt
     6181 cgtattatgg ctaccagtaa aatatgagcg gacacgattt tttaatattt tggctttgcc
     6241 gacataaatg atcgttccat ttttatcttt catcaagtaa cagccgggct gatcaggaag
     6301 taaagctaat ttgtttttga ttcgttcgtt catctggtac tccccttcat attcaattaa
     6361 agttattata gcatgtctgt caaaaaaaca atccagcctg acaacgtaat tatactcaaa
     6421 aaatattaat ttgaataacc ttaactcaac cgcaaaaaaa ccgaggacgg aactggattc
     6481 agtcccaccc tcagcttatt tttgctgtaa gtttttataa atattgatcg atcatttgac
     6541 gtagttgttc ttttgaatga acaccaacag ctttttctac tacttgacca tcttttttca
     6601 acattaatgt tggaatgctc atgataccga attgttgagg agtttctgga ttttcatcca
     6661 catccatttt agcaattctg aattcttctt catcatattc ttgttccaac tgatccaaaa
     6721 tcggtgcttg catacgacat ggaccacacc aagttgccca aaaatcaatc aatacaagtc
     6781 ctttatctgt ttcttcatta aatgtcttat ctgtaattac ttgtgacata tcaaagacct
     6841 ccatttttaa aatctatgac tagtatagca tttctatact tggatagcga accatctgct
     6901 tacctttagt aagctttact taaatttaac aattgtcgca ccatttccgc cttggtttgc
     6961 cggtgcaaac tcaaagcttt tgacgctccg atggttttta agataatcag taattccctt
     7021 gcgaagtgcg cctgttcctt ttccgtggac aatagtcact tgaggataac cagctagaat
     7081 ggcagaatcc aaatattgat ctacctcact tagcgcttct tcgtatcgtt ttcctcgaag
     7141 gtccaattga ttcggcacgt gactactaga ttcagatcgt acagcatgga taactcgttg
     7201 tgtcggctct ttttgaggag caacaggcgt cagctcgtct tcggacacat tcatttttaa
     7261 tataccgatt tctacttgcc actgattccc gtttttacgt aaaagcgtcc ctctttggcc
     7321 gtaagtcgta accaacactt cgtctcccgg tttcaacgtt ttttgttctt tcgccttctt
     7381 caatacttta tttttcttca aagtttcttc ttgatgtaga tttgctaatt gggtctttgc
     7441 atcgatcaac tgatgctctt tcacatttcc ttgcccaatc tgctgttgca ttttacgaat
     7501 atcagcaatg attttttctg ctttttcttc cgcctctgac acgacttcat ttgctttttt
     7561 ctttgctttt tccatttctt tttcacgttc ttcaaagaaa tagctgtaag cttctttcaa
     7621 atcatcgtga agttcttgtg cctcagaaac aaaatgacgc atttccagat attcggtctc
     7681 tgccattttc cggcgattct ctaaatctgt gatcatttcg tttaaatctt ggctctcatc
     7741 attcatcaac tgcttcgctt catcaataac ttctgtatca agacctaatc ggctagagat
     7801 ttcaaacgca ttgcttcgtc caggaacgcc aatcagcaaa cgatacgttg gacttaatgt
     7861 atccacatca aactccatgc tggcattaat cgtatttgcc cgattatatc cataaacttt
     7921 caattcagga taatgcgttg ttgccatcac ataagcagac tttttaccaa gatcatctaa
     7981 gattgcgatt gctagagcag caccttcttg cggatctgtt cctgcaccga gctcgtcaaa
     8041 taataccaga cttttttcat tgacttttga taaaatatct acagtgttcg tcatatgaga
     8101 agaaaaggtc gatagacttt gctcaatcga ctgttcgtct cctatatccg caaatacttc
     8161 ctcaaaaata ccgatttgac tttcttcacc tgcaggaatc ggtaatcctg cttgccccat
     8221 cagttgtaac agacctaaag tcttcaacgt gatcgttttc ccacctgtgt taggtcctgt
     8281 aatgacgatg gcttgatagt cttttccgat tgtgatgtca ttagaaacta ctttttcctg
     8341 atcgatcagt ggatgtcttg cttgtttcaa gacgacatga ttgtcttcac tgattcccgg
     8401 cacgatagct tttacttctt taccaaatcg tgcttttgca ttcatcagat ccattttacc
     8461 aatcacataa gcattgtgga tgatttcttg acgatgggga acaagttcag cagaaagttc
     8521 acttaaaata cgttgaatct catttcgttc agcgatttgg tattgacgca aacgattatt
     8581 caaatccacc acttgttttg gttcaataaa taaagtctgt cctgaagcac tttgatcgtg
     8641 gactacaccg ccaaatacac cgcggtattc ttgtttgact gggataacgt agcggtcatt
     8701 tcgcatggtg atgatcgcat cacttaaata ctttgcattt ttcccacgga cgatcccatc
     8761 taactgttca cgaacagctt gttcacttcg ccgaatgttc tgtcttatgc tctttaactc
     8821 aggagaagcg tcatcagtca cgcgtccgtc ttcatcgatt gcttccttta aacggcggct
     8881 caaaacaggg atcgtgacta actgatctgc ccacatatac aaacgtgcaa attcgatttc
     8941 actgtcataa agatcatcaa tgaaacgatt caattcgctt gtcgtagaca atactctgga
     9001 aacttgtgca agctccactc cattcaagtc tgctccgatc tcaatccgtt tcatatgggg
     9061 acggatattt tcaagtttag gtatagggat cccgcctcgc agacgttgca ctttcaatcc
     9121 atcctcagtt tcttgaagcc agtttgtgat cgtttgttcc tcgctcactg gtaccaattc
     9181 tgccagttct tcttttcctt gtgcagtcac tacaaattgt ctgaccatct gtttgacttt
     9241 ttcaaattcc aatgtttcaa ggattcggtt attcatttta tcaccttatc tttcactatg
     9301 aaaaaagagc tgtgccggac atacgctacg cagccttctt aaataatcta ctctgtacaa
     9361 aaaacgaaat attttccagc actattttcc ttaattcaaa atattcgtta cccaaagctc
     9421 atacatcttg ttggtaagaa atggcgttct ctcaacgata aagcgagcga aactatgctc
     9481 gccgaattga ttttggacca catctaatgg aacaaaggca agtaactgca aaatcaaaaa
     9541 tatagtaacg taagccatga ttagtgacag gatgcctcct gcaatccagt ctacctgtct
     9601 caagatcggc acatacgtca atccatggac aaaaaccccc acaaatcggg taagcagcca
     9661 gccaattagc aaaataatca gaaaagcagt tcctgcataa aaagcttcat ctaggtgaaa
     9721 ggcaaatacc tgatcaaaaa atgctaactt tgatgttggc gtgacagctg ggtatggaat
     9781 gtataattcc aaatgacttg ctaatggttt gtaaaaatgc tgggcagcaa taaatgacaa
     9841 tacatatcca atcgcgaata tgatttgcaa agcaaacccg cgacgtgctc cagaaaaaaa
     9901 cgcaattagc aataaaaata aaatcagcaa actcagcatg ctacgttcct ttctcttcta
     9961 acttttttta aatttttctt attttgataa aaggatctta attagaagat ttttgtttaa
    10021 tttgtccttt tcgtttctcg tttaaaatct gttgtgcttc cacatgattt ttaatggtat
    10081 gatctgcttg accattttct ttcaatactt cccgagcttc gttctcgatt gcttcaatgc
    10141 gtttcacacg attttcaagc tctgtaaatt tgatcatttt tttctttaat tctgctgttt
    10201 cttcttccag ctccaagatt ctttcttgtt tcttcaattg atccgaaagc gcattgacag
    10261 ccatcaagat tgctgcttgt tcattatcca tttgtggtga taattgtttc aattctgcta
    10321 actgttcatt gatcagcttc gttacgatat ccatatgatg cttggtttcc cgtccgataa
    10381 tagtatatgt ttggttggca atcaccgctt tgtagcgtgt tttttcgtgc gccatacgaa
    10441 tcatttcctc ctatttgatg aaagttttat ttagtgtacc atgaagttgg ttctttcacc
    10501 gttctccaca aatatttttc acatacttaa ctattatatg ataaacttta aaaaaatgct
    10561 atgtctcgca atctcttaga aaacgaaaag tcgtgagttg cggtatagtt ttatgtataa
    10621 aaacgaggag aattttatga gcaacgtcat tatcaaagca acaaacgacc aacttagtca
    10681 aatgaaagca tactatcgct cttatttgtt aaataaacag attccttata ctagctttgc
    10741 tgcaaaaaaa gacgggacga ccattaccgc ttatacatcc ggaaaagtga tgtttcaagg
    10801 tacgaatgca gcaatagaag ctgataaatg gggggaggca caggataaac aagccaagcc
    10861 agcaaaaaca gcctcttctt tgccaaaaaa tatcagtcaa ttatccgttt taggtagtga
    10921 tgaggtcgga aatggcagct atttcggtcc gctaacagta tgtgctgctt atgtgaaacg
    10981 tgatcagctt gcagaattac gtaaacttgg tgttagagat tcaaaagagt taaaagatag
    11041 ccaaatcatc cagcttgctg acatcttaaa aaaaaccatc ccctataaac tgcttgtctt
    11101 atctccagaa aaatacaatg agatccaacc aaaatacaat gccgttcgga tgaaagtcgc
    11161 tttgcacaat caagcgatcc atttgctgct aaaaaaaatc gagccagaaa aaccagaagc
    11221 gattttgatc gatcagttta cgccggaagc aaattttaaa aaatatgcgc gtttagaaaa
    11281 aaatcaatta caagaaaaac tgtacttcgt tactaaagga gaacaatatc atttagctgt
    11341 tgctgctgct tctatcatca gtcgtgccag ctttttggaa gagttacaca aagcatcagc
    11401 tgaagctgga atcacccttc catcaggagc tggaacgaaa tctgaccaag tagcggcaaa
    11461 actattggag aaaggcggca tgcctatgct tgaaaaatat gccaaacttc actttgcaaa
    11521 tacagaaaaa gctttgaagc tactaaaaaa ataaacggtg ttcaaaagct tgcatctgcg
    11581 ataataagcc cggccaaacg aaaaatatga ggagctattt ttgtttggct gggcttattt
    11641 attactgaaa ataagtggtc aatgcttgtg tcaaacttgc agcaatctgc tgctgataac
    11701 tgtctgttac taattccttt aaatcactat cattattcat ataacccatc tcgattaaaa
    11761 tagaaggttg aagattttct cgaagaactt gatagttccc agtggagatt cctcttgaat
    11821 ccagaggcag atcagtgaag gagtcagcaa ttgtctgtgc taatgtttcc tcttgtccat
    11881 agtaataata tgtcgtgatt ccagttgctt cattcgcata ttccgtagag tctgcgtgta
    11941 aactgataaa gacatctgcc tttttttctt cactgtagtc gcatatatct ccaagctgta
    12001 ctaattcatc tgtagtatgg gtcaaataaa ccgttgctcc agcatcctct agcgcctttt
    12061 ttacttttat ggctgtactt aatgttatat ctttttcttc tgtttgttca tcattgctta
    12121 atgccccagt atcatttccg ccatgcccag gatctaatac aatcactgca tcagatagct
    12181 cttccggttt tgtttgtata ttctttgtga ctttggttcc atcttcgagg gctaaatatc
    12241 cagtatagcc atcatttgtc ttgatctgat aagcattttt tgtttcagaa agaaactccg
    12301 ccagctctcc gccgtttata gttgcaagtg gggtagttag cgagtcatct gtatatagtg
    12361 tacggtcatt ttttccgatt ttgattaatg tctcttgttc tgtatcatca aatgattcct
    12421 ttgtctttgt aaccgtactg ttcgtactgc tgaccgtttg tccaactatc tttttttgac
    12481 caaaaacatc tatttgaaaa cagataataa tgataatgat tgcaaataca aaagctccaa
    12541 ttattttttt cattcttgac tctcacctcg tagatccaga ctaagggcca aaacttaaaa
    12601 tcagcttaag taagcaaaaa aggggctgta aaagatataa ccgtagaaaa taacattact
    12661 gctaaaatga tttttttcat ttttggcata tcctgaaatc tccttttgac tattcgtggt
    12721 gattcatcta cccaataaat agtttccttc attctacaaa caattaattt cttcgttatt
    12781 aaattaaaac acttcctgga acaccgacat aataaataaa aatataccct gataatcatg
    12841 agagaataac aaatttgtct ttatgaaaaa ctacctatat aaagaagcaa gatataacga
    12901 tcttcgtcat atcttgcttc ttcacctaac tattttctcc ctatataaca gctttacgct
    12961 tctcctaata caccattcaa atataaatcg atcaattgcg ccacatttag cggttcttcc
    13021 acaggtcttt tcgaccgagt gatgtaaggt gctaacacta agaaaaaatg ccttgctgcg
    13081 atttcttttg aaatcccatt tcttagcaca gattggtttg ctgaaaagat ttcttcaaaa
    13141 ggttcacgat aatttttctt ccatagaaag aaaagatgat ctcttgtttc ctttgaaaga
    13201 ttgctttcaa gatcgtgaag catcagaaac aaatcgacca gatgtgtctc aatcaaaaac
    13261 tgcgccattt ttgtcaaggt tacttgaaat cccgtttcgc ttgtttgaac gatttctctc
    13321 aaagcttttc caacatcttt taagtggttt tctaaaactg ccgtatatag tttttcttta
    13381 tcaccgaaat aatgatagag attgggctgg gtgatcccaa ctttttgtgc gatttctctt
    13441 gttgaagtgc cgagatatcc cttggacata aacaagtcag tcgcttcttt caaaattttt
    13501 tgctgtgtat ctttgttgta tcgatggacc atttttccac ctgctttttg gttattcttt
    13561 ttctttcaat acaccatctt ccatcataaa tattctatcg catttgtcaa tcagccgtgt
    13621 atcgtgagta accatgatcg ttgctttatt tttgtctttt gattcttttg ccaagatatc
    13681 gactacttct cgtgcctttt ctgaatccaa actagcagtt ggttcatctg ctagaataat
    13741 ggacggatca ttgtacaatg cacgagcgat tgcgaccctt tgccgttctc caccagatag
    13801 ttcttctgga tacttgttga tcaacttatc cacgccaagc tgctcaaata attcgtttgc
    13861 ttctttttga cgatgttcat gtttaatctt atctactaat aataactgct tttttactgt
    13921 cagaaaagga accagattgg aagcttgcaa aataaagcca atttctttaa aacgtagttt
    13981 aacgcgttcg ctttcttttt gattactgaa ttcttgatca ttgataaata cagagccact
    14041 gctaggcgtc tgcaatccgc ctgccaaagt caaaaaagta cttttacctg aaccagaagg
    14101 accgatgatt gcaacaaatt cccctttatc gatactcatg gttgtttctt tcaatgcttt
    14161 aactacttgg tcgccatctt ggaaactttt ttctacttct ttaaattcta ttgctttcat
    14221 gttcttcctc cttcttatcc aattgccttc aacggatcga tttttacgat tgtccgaaca
    14281 gaaaatagtg cgcctaacac agctactact aacatcaaca agctaatgac gccaaaaaac
    14341 agccaattgc tttggaatgg aaccgcatca ggtagaacaa gggatgttcc cactgttcct
    14401 aatagaccaa gaagaatacc aacaaatgat aaaatgaatg tttgtgccac tacagaacgc
    14461 gcaataaatc ctcctgtgat cccttgtgct ttcattacac caaagatatt aatcttttgc
    14521 atcgtcagta cataaatgaa aatcccgatc acaatagccg caataacgat caaaaatccg
    14581 atcataaatc caaaagtcaa tacttgcgca ttatatccag gtaattcatt gatgaattta
    14641 gaaattttga tctgttctaa atcttcaggt aaattactga ttttacctct gacaactatt
    14701 gcgttaatcc gtgcattttc acttgtatct tctttttcga agcggatttc ttggtaagca
    14761 ttgatggttg tatagagaac tggtgagaca ttgaatttgg catgatccgt aaagccagtt
    14821 agtttaaacg tcttgtcact accagataac ttaactgtat ctcctatggc tagaccatat
    14881 tcttctttca aactaatatc gcctactgcc tcatcatcgt tatcaaaagc ttttccttca
    14941 acaagattgg gcatgatgaa ttgattttta tcgataccga agaagctaac attgattttt
    15001 ccttcttctg tactgtcttt actagtcacg actccaggtg tttgggcaag atacgccact
    15061 ttcccacctt cgacttcttc tgctatcttc ttagtaatca tggacatccc tagatttgaa
    15121 tttgcatctt tcgaaagcac aatcgaatct gcctcccact tgtcgactgc tgttcgatta
    15181 tcttgagcta agccataagc taacccagtc aaaaaaaata ccaggtaggc gatcaaaaac
    15241 atcacccctg caactaacgc ataacgtaac tttgaatgca taatttcatt taatgctaaa
    15301 aacatttaac tcctcctcct gaaataatca ttttatacaa caattttatc acttgataaa
    15361 tttttggcga atattatgct caaaaaattc ttccgctatt tcattatatg aattttttat
    15421 tctcgattga ttaatattta ttatatcccc acacaataaa agaccgtgat atttctcaca
    15481 gtcttttatc tactctattg ctatttgctc tcttagctag tactttaaaa gctaactcat
    15541 tgaggtagtg aaaacgagag gtgtattcag acagaaatcc aaaatcctca actgctagct
    15601 gtggaatcgt agttttgtgt ttcacgatat ccgcaagcag gttcaaatct gataataaga
    15661 attcttggct tatcagctgt ccgccaatta tttctaatgt atccggatgg tagatcagct
    15721 ttgcatttac tgttttgttt tcttcgtcgt attgtggaaa tagatcaaat tctcctgaac
    15781 acgaaccggt attttgttcc aagaatggcg cttcatcttc tgtcagccct acagttccta
    15841 aaaagtagtc tataatgact gctgctgtta tcttttgtct gaggtcgtaa gcggttggct
    15901 gaccactcaa agttagcgct actgctcttg ccatatgtac agctcgagta actaaaggta
    15961 aaaagagatg ccgtttattt ttgtcaaaac tgactgggac gatatctcca atcgcataga
    16021 tgtccggatc acttgtctgc agatattcat tgactatcac agtctcatcc tcatacaaag
    16081 ccactttttc ttttaacaat gtcgtattag gacgagtttg ggcagaaaaa atgaccatat
    16141 ctgcctttat tgtttgagac agaagttgta cggatactac tttctcttct gattttttcc
    16201 aatcgatcaa gaattcattt ggataaaaat ttatttgctt agtttgcagc tgattttgaa
    16261 aactgttgat catatcctta tcgaaatatc ttgataaaac attcggcata taatcaacta
    16321 gatgaataat gtatccttta tttctcaaag catctgctaa ttccaatcca atgtaaccag
    16381 caccgaagat cactacttct tttgcttctt ttaaagcggc aagtacttgc ttagattgca
    16441 ctagattttt ataggtgtag acattttcag cttcaatttg tttatacaag gtgacaggag
    16501 aggaacctgt cgctacgatt aatttatcat aggttatggt ctcgtttttt ccttctttgg
    16561 atgcgaggat gagctgatgt ttgtctggat caagttcagt tactctccat tcggtgatca
    16621 gttttgcccc actttcttga agagtccgca tgttatgggc aacttcagct aaactacgta
    16681 tctttccttt taaataaagg ttgattccat ttgaaacata gcctagtcca tctttatgat
    16741 cttcatcgat cagtaaaact tctgctttcg gttgaagttt tcttagattt agggcagctg
    16801 tgatgcctgc atgggaagca ccaatgatca cgattttcat gttttattca tccttaaatt
    16861 caatttttct aaataacatt cttttaaaaa ttggttcaaa taggggaaat cataattata
    16921 ttcgttgcta gtgaatacaa aaactttagc ggacttctgt gacgggtact ctttcaattg
    16981 gctagtcaaa atgatatcat actgcttttc tttctcgtat tcgactaaat catatttgat
    17041 cgtattcagc aattgttcct ggatgatttc tttcacgata tccgccatta gcctttcata
    17101 aggaagatca taagcaattt gcagacgtaa ttctaactgc ttgatcagcg tccgtaaaat
    17161 agatgcatag cgagcaaata attcaaagcg ggcattttcc tcgtctactt cagtaaaatc
    17221 aacaatagca aatgtttgtc tgaccaatct ttcacataaa accaggaatt gtttatcttc
    17281 tacttcagtt aaccgctgtt ttaatccttc ttggccaaaa atattcatcc atcctctaaa
    17341 ataggcaaaa tgagcgtgat tttgcatcac tgtcaacgca atcttttgcg aaatctcttc
    17401 tggaaaaaca taaaaagaga aatattcttc tatcgattgt ttaaacgttc gattcaattg
    17461 agctactacg tcgtctttgt atttttcgct tatcaaaaac tcctggacat gcgagttatg
    17521 tgtatctaat gtaaagttac tgatcatgaa aagataaaaa accaacgttt cattttgatt
    17581 ccaaggaaag gcatattgaa agaagtaacg ggtcaaaaat gtatggaggt ccaataaaaa
    17641 ttgtgtcgtt tgaaacaatc caacaggaag attgctataa tctggtttgg attttttcgt
    17701 acagcgcatt cgcttcttca taatggaaag ccatatttca aatttcaatc gtccttcttc
    17761 tgtaatagca aaactaaact ctttttgcag cagatctaaa aaagtgtttc ctgataaatc
    17821 actgtattct tttttgtttt caagataggg acgtccaaac caaaataatt ggaaataaaa
    17881 ataacgaatt tgactttctg gaccggtcag ccgcccgctt tttattttca gatgaaactc
    17941 atttaaagaa tcatttaatt ccttcactct gcgagacaaa gtagccgaac tgatattaag
    18001 ttccatgaca agtttctgcc gatcaaatga gccattttcc aacagataaa gaagtatctg
    18061 atattttgta gaatagcgca agtaacaatg ataataaaca tcgactgctg ctatatttcc
    18121 atgtctgatt aataaaatat tcccttcttg gataacaatt tccaattgtt tgatattttc
    18181 caagttagct tgtaaataaa acagatcttc tttcaaggta gcccgatcaa ctgataagcg
    18241 ggtaagtacg ttattaacgg aaacctctcc tttttcctct aataaaagtt ggaaaagttt
    18301 gatttgccgt tgttcatttt tttcaagcaa actgaaaagt tccaagtcca ctccttcaag
    18361 catttctgat tttaacagca gaagtttcct tctcatcctt gaaaaagagg atctgtgaca
    18421 gttatcacag atccatacaa attctaatca cttagctggt tggcccagct gtcagtttcc
    18481 atgtgaccat gccttgatac gttgcgccag gttgagcatt cgtactgcta tttacctcta
    18541 agagtaaacc atcggtatcg ggaaagtcaa cgagaacctc tcctttagct gttccatcac
    18601 tctcataaat caacgtgttt tgaccttgct gaatcaattg ctttgatcca gaatgattga
    18661 agtataattt tgttccgctt aattcttgac cttgatcatt cttaaataaa gaagtcaatg
    18721 tacctgtcag ttgccaagga gacgtgcttg gaagccgagt atcccgcaca tcaaaagaaa
    18781 aatcgccttt tgattcataa taacttgtct tcaacggttt ggggagatcg ttaccaaatg
    18841 taaaatcatc tggtacatta actaatgtta attcttgttt ggggacaaga attgttttag
    18901 cagatgtttg gacaactggg ttaggaagct tagcaccatc tgctaatgta atattgctgg
    18961 aaagtacagc tgcctttgtc agtatgctga cggattgtaa agaagtatct acagtattta
    19021 attgatagtt gtaagtaaga tgaatggtat ctcctatttt gctcaataga tctgctggga
    19081 cagaaactcc ttgttgaatg ccggtatctg cgatagttgt accatttaat tttaaggaac
    19141 cattgatata agtagtattt tcaggaagtg agatcgtgat ttttttgtcg gttatcgcat
    19201 ccccagattt tgttaacgtg aatgtcacat tttcttgaat cgtatctcct gatagagcag
    19261 acaggggatc tcctgtctca ggttgtctgc taaggttttt tagctgacta tctgcactca
    19321 cagatggaaa attagccact gttatttgag gaagagtggt gattccagct tgttcagact
    19381 gccatttgta cgcaaaagac ggatacatgt tagtaagtac atcgtccacc aagcgataat
    19441 caaaataata ttgccctgaa gccaatgcag gtagtgtaga agtagctgta tttacacccc
    19501 cgttatttga ttggatcgtt tggaaaagtt gatctgtcca tcctgtcgct aatttttggg
    19561 ttcctttcga ataaagcctg taatggatcg aacccttttt tagttcagtt gctgaccaat
    19621 tggccgtgac ttggtacgat gcagaagaaa ggagctttcc ccactgacta tcggctgtta
    19681 agtttgttgg taaaaccgtt ccgtctagct tgtactgctc caataccttt aaagcaacag
    19741 gagaaagcaa tccgccttgc gcatctttcc cactgtacac gatattggct gtctgattca
    19801 atccacttgt gcttgctgga gtatgagtca gtattgctcc ttgtgtaacg ttttttcctg
    19861 taaaatagtt gactacgcga tcaaagcgac taaatggcaa ctgatcaaaa cgtccttcag
    19921 tcagccccat cgtcaatgtc cgactattgg tagagcctgg aaggttctgg ttatttccat
    19981 ccacgtattc tgtggtatca gcaaatgtat tatattttcc tgttgaatca cttggtgaga
    20041 tgtaactatt ccacgtattt atctctgcac ttgcagaaaa tgcatatgct cctggattca
    20101 agtttccgcc agttaattta ggataaaatc tacctgcagc tggagctaaa atcgaattga
    20161 acatacgatc ttggataata ttattcgaat cataaaaatc aacagaaaaa acggcctttt
    20221 tagacataga aggttgtaat ttacccttgg tcaaaatttt gtttggactc gtatcatatt
    20281 tatctccgtt ttctgtcatc ttcgcgacat tagctgtaac atcaaattga agcgggatat
    20341 cgtccacgtt gaattcgcct cgcgtaataa tagcaataat atctgtcaat agagtatttg
    20401 tatcccacga ccccatccca aaacgcaagg tatggcttcc tgctgataat aaacttccgc
    20461 tagtaacctt gatgggggca ccggcaatat acaagtaggc gttgttccag tcaattgctt
    20521 ttaatacatc actgcttgat acatgttccg gcaaattgat gatcgccaca aatgcaaatc
    20581 cttgtgcagt gatattcacc aaatcaagta catttgttag tttcacatgg ctttcccaat
    20641 gaaaacggac cgtgccacct ttccaaggat catttgaacc atcgttcaaa taagtagcag
    20701 tcaatttagg caattggtta gccccagtta gttggacaag cttcccgcca ttgactgcac
    20761 tacgtccacc atttggaggt acgatatttt catacgtgta tggatcataa gaaatggcag
    20821 gaggcgttgc tccaacagct tctgccgaag gtgaaaagat aaatatactt aaaacaaaaa
    20881 gcgaagtaaa aaagaaggag cgaaaaaatg aaaatttatt ttgtagcaac atgttcatcc
    20941 tccttttcct ttcttctcca taacaacaac cacgcaagaa gccataattc tataccaatg
    21001 atcaaataaa gaaaacggat cttttgttca ttcgtctccg ggaatacgcc atgttgatta
    21061 gattctttat aaatcggtag ctgtgtctgc tgttttgatg cgccattgac tgaaccactt
    21121 ggatgcggaa ttggacttag atcataaaat gtaatcgtac ctgaactttt ggtatctgct
    21181 tggactggct cttgccagct gaagaacagc aaacttgcaa taatccctcc caataagatc
    21241 gatgttttca acgaaatcac ctcatcatgt tgtttctcct tttttctttt tcttgtggac
    21301 ccacaaaaac agcaaaatca accataacac tagaactgca gcaaccaatc ctgcataaag
    21361 ccatgggaaa gattgttctt gtactgcaaa aacggaattt tcatttactt tttgtgcttt
    21421 ttcttttttg atcgtaaagt tttgtgtaaa tcgccattca ttactgtcgc ctgattttgc
    21481 tatgatttct gcgcggtaat cgccggcttt tgctttatca ccagctaaat caagattcaa
    21541 gtgaaaattc gaatttggtg caactaaaaa tgcattagac gtattttcaa tataaagctg
    21601 attttcataa aagatcttgc tcgtaaatat caaattgctg ataatcctag gctgaggatt
    21661 ttggaaacga agctgaacag ttggctttgc gttgacctcg tccaaatctc ctgataaaag
    21721 tttcagatcc ggttctatcg tctcttgact ttcttgtaaa acgactgcga gattataagc
    21781 aatacgattt ttgatttgtg ctccttctgt agcattttct gtttcagctt cttctttagg
    21841 tgaaacatga atacccgcta atacacgtcc cttaaagctt tttgctggga ttttcacttc
    21901 tataggtaca gttgtttctg catgtgctga tacattgatt tcctttttcg caatgagcaa
    21961 aacgtcacgg acatcaaacg gcaatgtctc atctaacgtt gggttttttt gactataatc
    22021 gatcgttcca ccatcactcg ttaccgccgg attgacagag acctcaaatg ttcgttcttc
    22081 atctgaagaa ttgctgataa ttacttctag tgtctgattt tgttccggtg ccactaataa
    22141 atcaaaataa ccgattgctt tatctatttg gttctctgga aataccggtt tgattccaaa
    22201 gtctcctgct gaatcctttg cttggctgat tgacggaaaa atgaaaaata gacttaaaat
    22261 agaaaaaaac gaaacaaaca gtcgtttact ttgattcatt gtccaccaca tcctttcaac
    22321 aaaaactgcg gtaaaaaatc cttcacctat agtattttca ccgcagtttg ttgaatttat
    22381 ttcatactct atgctactgg gccagccacg aggttccaag tcaaatcagc cgtgaatgtt
    22441 ccagctgctg ctttcggcac tttcagtgtg atatttgctg gatcataaac agaaagccat
    22501 gttcccagac cttgatcttt tgctgctgta aatattggtg tcgctgcact tgctttactc
    22561 aacccagtta cagtttgact agttggtcca gtggattcgt tatctgacgt acttttaaca
    22621 gtggatgttc caaaatctaa ttcagcgcct tgcaaaactt gtgaaccgtt tacaaaatct
    22681 gaaaccgaaa ctgtcacgtt ccacccttgt gcttggccat ctgcacctac acctcgacga
    22741 tctgatactt ggagataagg tgtatcgttt tttgaagcgt ctacttgata cgtttttgtt
    22801 ccgctttcaa tctcgtgtgt accaaatggc aattcgggaa ctacatccag tgttaaagga
    22861 cctgtctggc cagttgctgg attttccgga tctgctggct ctactggttt tgaaggatca
    22921 gtagggttaa gaggctccac ggtatttgtt ggttcagtaa aggtaatatc tccttttgaa
    22981 gtcgcttgac cctcagcaaa agctggtgtt gccaagccgc ctagaagaag tgttgaaagt
    23041 aataatgtcc caatcatgtt ctttttcata ataaatcact cctttttctt agtgacctaa
    23101 gtatagtaga gacagtatgc aaaaaatcct catgaattca tccgcttact tgaaattatg
    23161 accctttttg tataaaagag aaaaaaacaa taaaatcgtt tacatgaata ttcttccttt
    23221 tttattaagt atgaaaaaat atttcaattt tattttttcg tttcaaatta ttctattagt
    23281 gacatccttg aactataaaa ataggagtac ggcatcatct gccgcactcc tatttttttc
    23341 gtcatttgtt tcttaaataa aacgattctc acgcaatttt atttttttga tatcttctac
    23401 tgtttgtgtt ccagctaatt gcatgaccat ttccaattct gttttaaaga aatcaaacac
    23461 ttgatggact cctgtacttc ctcctaaaga caatccataa ataacgggtc tgccaatagc
    23521 aacaagatct gcacctgatg cgattgcttt gaaaacatgc tgtccgcgtc gtacaccgct
    23581 gtcaaacacg attggtacac gtttatctac tgcttctgcc acatattgca acgaatcaaa
    23641 cgctgctgga ccaccatcta gttgacggcc accatgatta gatacccaga ttccgccagc
    23701 accagattct aatgaacgat agacatcttc ttctgactgg acacctttca catagactgg
    23761 caaatcagaa tgagcagcaa taaattctac atcttttgga ctaagcttct gtttggaaga
    23821 tttgtacaca gcatccatcg tttggcctac tcctgattga taagcctgaa cgatcggcat
    23881 tggcagtggg aaggtaaacc catttctgcg gtctgtttca cggtttccgc caactgttgc
    23941 atccgctgtc aatacgattg cttttgctcc attacgcttg gccacttcaa gaatatccaa
    24001 attgatccca tcatctttgc tcatataaaa ttgaaaccat tgcggtgctt tctccccgcc
    24061 tgcttctctt atctcttcta atgtacaaga agcgtaagaa cttgctgtat agatcgtccc
    24121 aaaatctgct acaccttttg cagaagcttt ctccgccttc acatgagcta agccatgagc
    24181 agcaactggt gccatgatga ttggtgcagt cagcgtttct ttgtcaaaat gtgtagttgt
    24241 atcgggtaat tctacatctt ttaatacatg aggaataatc agctgatgat tgaatgcccg
    24301 ttcattttct tgataagtaa ataagtcgcc agcaccactg ctgatgtaac cataaccacc
    24361 agctggaatc acttgtgaag cggctatttc taaatcttcc atattgataa aatccactat
    24421 tccttcattt gtacctgctt gatacgtttt ttccatttca atgccttccc tctataaaaa
    24481 attgaatgaa taatatgata gcgtttacac actttatatt actcttccgt attgatacag
    24541 gcaaataatc tgtttacatt tcagtcattc cagttgttac tactctgaaa gaaatgttta
    24601 gaacacaaat agataaaaaa cgatgggact ttgacaaaag tcgtgtcaaa gtccctttta
    24661 cgaaatactg tggatgccta ttgaaataag tgcgggttgt ttctttgtat ttcaatcgtt
    24721 tcttgccatt cttcgtttaa ttttggatta ccgttttctt ccttgaacgc ataaggtccc
    24781 aatccaaaaa gctcagttag tatttgattc acgattccat atagctcggt gtgatcggac
    24841 acatgattat tgaagatcat ctcattatca ccacgtttga atctcacatt cgggctctca
    24901 tgatgaacct ctatgcagtt ttcccctttt tttaactgac ggatttctga aaacatctca
    24961 taaatatctt ctccaataaa accttcagca agcgccagac tgtccacaag accgcctact
    25021 gtcaactcag agattatcgc tttgttggca ccaagcttta atgtatagct tgttgcttct
    25081 actacgccgc atgcattgca accaccttct gatagttcgc tttttctgat caattgaact
    25141 tctttcattt tctgttcctc ctcttagttt tctacttatg tattttttat cacctgacgt
    25201 aagtgtattt tatcgtcacg atcacgtgat ttattcttca gagattctat ttatctatct
    25261 aacatatcct ttattcttct acttttcttt ctttagcata taagagatac gcatagtaat
    25321 caagcaagga ttgttgatat aaaaagaaca aataccgcta ttttgatttc ttttgactct
    25381 attactcata taaataaaat ttattatacc tgctcttttt gactatatcc tcatctgact
    25441 cctatacttc gtttgtatta aaacttagga ggaatcattc atgcttacag ggctaaactt
    25501 attaaataat ccttttctaa ataaaggaac agcttttaca aaagaagaac gggcaaaata
    25561 cggaatcact ggtatgcttc caagtactgt ccaaacattg gaacaacaat ctgtccaagc
    25621 ttacggtcaa tacttaagca aacagaccga tttggaaaaa cgtatcttct taatgaatct
    25681 attcaatacg aaccggacat tgttttataa attgatgggc gaacatttag ttgaattcat
    25741 gcctgtcgtt tatgatcctg tcgttgcgga ttcaatcgaa caatacaacg aaattttctt
    25801 gaaaccacaa gatgctgcat ttctttcaat cgacgaacca gaaaatatca aagcaagttt
    25861 gaaaaacgca gcagatggac gcgacattcg tttgatcgtt gtgacagatg cagaaggaat
    25921 cttaggtatg ggtgactggg gtgtgaacgg tgttgacatc gctatcggta aattgatggt
    25981 ctacactgct gcggctggta tcaatcctgc acaagtcttg cctgtatcaa tcgacgctgg
    26041 tactaataac gaagaattat tgaataatcc attatactta gggaatcgtc atgctcgtgt
    26101 agaaggcgaa acttactacg aattcattga tcaattcgtt caatcagcta ctgaattatt
    26161 cccagaattg ttattacatt gggaagattt tggacgagga aatgctgcaa cgatcttaga
    26221 aaaatacgaa gataaaatca ctactttcaa tgatgatatc caaggcacag gaatcgttgt
    26281 actagctggt gtattaggtg gattgaatat atcaggtgag caattgaaag atcaaacgat
    26341 cttgacattt ggtgctggta ctgctggtgt aggtatcgca aacatccttt tagatgaaat
    26401 gatccgtcaa ggtgtacctg aagaagaagc acgccaacat ttctatcaag tagataaaca
    26461 gggtttattg tttgaagata caaaaggatt gactcctggt caaatcccat tcgctcgtaa
    26521 acgctcagaa ttcactaatg ctgatgaatt gacaaactta gaagcagttg taaaagcaat
    26581 ccatccaacg atcatgatcg gtacttctac ccaacctgga gcattcactg aagaaatcgt
    26641 gaaagaaatg gcggctcata ccccacgtcc aatcatcatg cctttgtcta atccaacaaa
    26701 attggcagaa gcaaaagcaa aagatttgat tgaatggacg gacggaaaag ctttagttgg
    26761 aacaggtatc cctgctgctg atgtggaata taacggtgta acttaccaaa tcggtcaagc
    26821 aaataatgcg ttgatgtacc caggtttagg gcttggtttg attgcttcaa cagctacacg
    26881 agtaaatgct gaaatcattt ctcaagctag tcgagcatta ggcggaatcg ttgatgtaac
    26941 aaaaccagga gcagctatct tgcctccagt agcaaaaatc actgaattct ctcaaacgat
    27001 tgctgaaact gtcgcaaaat cagttgttgc acaaaaattg aatcgtgaag aaatcacgga
    27061 tatcaaagaa gctgttgaat ctgctaaatg ggttcctgaa tataaatctt tagaagatta
    27121 agattgttat agtaaaagaa catacagaaa ctttgtatga cgtctaaaaa gaaaaaagcg
    27181 actgcggcaa aacagcagcc agccaactac tgctcttttg ccgcttctct ttggcttttt
    27241 tcatacgata cagaataaga aaggatctgt aaaaatggca caaaaaacaa caaccgcaac
    27301 tgcagttgag gaaaagaaaa aagaagggtt ttggtcaaca aaaattaccg gcgtaagttt
    27361 gcccctttat ttgatcatgg tccttgtcct gatcattgca atggctttag gaaaattacc
    27421 gacgaatatg attggcccaa tcgctgtttt ggtcattttc ggtaatttat ttcattttat
    27481 cggaacaaaa atccctatcg tcaaaagtta tttaggtggt ggctccgttt ttgcgatctt
    27541 tgcctctgcc gcgattgcta catttgggat cttacctgag tatgtagtaa aatccacaga
    27601 aaactttgtc agcaatatgg gctttttaga cttttatatt gctgccttga ttactggtag
    27661 catccttggt atgaaccgta atctattgat gaaagcttct gttcgtttta ttcccgttgc
    27721 acttatttca atggtcgtct ccttttttgc cgtaggactc gtaggtatgc ttatcggaaa
    27781 tggttttgca aattccgttt tatatatctc attgcccgca atggctggtg gtgttggagc
    27841 cggagctgta cctttatcaa cgatttatgc aaatgtttta ggtacggacg cttcttctat
    27901 catttcccgc ctgattcctg cgacagcaat gacaaatgtc ctagctatca ttggtgcagc
    27961 gttagttgct cgtgcgggac aatcaatgcc aaaattcaac ggtaatggta aattaatgga
    28021 aaaaggcgac cttggcgatg gaaaacctag aaatgtaaaa ccttccattc cacaattagg
    28081 tgtcggtttg atggtatcat tgactttctt catcttagga caaatctgta actattttgt
    28141 tcctaaagtc catgcttacg cattcatgat cattatcgtt gttatctgta aaatcgcaaa
    28201 cctattacca gaatattacg aagatgcagc tatcatgttc aacaacttga ttgtcaaaaa
    28261 cttgacagca gctgttcttg ccggtatcgg tattgcgtta ttgaatctaa atgtattggc
    28321 ttctgctttg acatggcaat ttgttgtctt atgtttgaca agtgttatcg cgatttcagt
    28381 agtttcagga tttgtcggtc gtttgtttgg tttatatcca atcgaatcaa ctattactgc
    28441 tggcttatgc aacaactcga tgggtggtac aggaaatgta gctgtcttat ctgcagctaa
    28501 tcggatggaa cttattgctt ttgctcaaat gggcaatcgg cttggtggtg ccatcgtttt
    28561 gatcatctca ggtttcttga tgcaattact ttcttagtca aaaaaaatac ctgatcatca
    28621 agtatcggct tgatgaccag gtattttttg ataaaaattt acaggatttt caaaaatctt
    28681 ttttgcattt tatcggaatg ataaccgatg cgttcataaa atcggtgtgc ttctactctg
    28741 tattctgcag aattaagtct tattgctgat attcctcgtc ttttcgcttc ttctacgcca
    28801 gtcatcaagg cctttccgat tcctcttttt tcagcatgtt tagacacagc aagtgccata
    28861 atattgaaca tcggatcgct gaaaatcgat tcataggact ccgcttgtac atagccagct
    28921 gcttttttag aagtaggatc ttcatagacc aagaaaaaat ttttttctgg ctccttcaac
    28981 agtttcttca tttgtctcac cgtaacggtt aatggtacat catatcctaa ttgttccgca
    29041 ttgatttctc gtagtgcttc tgcatctcct aaacgcattt cccgcaacat ccaaatcacc
    29101 agccaatttt tattttttta aatgaatttt tttatcacag tatactcctt ttttcattta
    29161 gaacaatatg tttcagtgat tttgacttgc gttagtctag attctcctaa tgtttgtaag
    29221 atgacaaagg cattgtaaca attgaccaaa agaaccacga ggacaattac ttgaaaaata
    29281 aaggtcactt tttttggaga caaaaccctg ctaaatcttg ctccccacaa acctccaata
    29341 acagcagcgg ggataacata aaacaacata gataaatcaa agcgcataaa gctacttgtt
    29401 aaagcaatcg ttaccaattt tgccagttga gaaaagaaaa tcgtagatag agaatatagt
    29461 gttgcttctt ttattggcaa tgcaaacatc agcatcaaca aagaaacatt gactggtcca
    29521 ccaccaatac ctaaaaaact agctaaaaag cccagaaccc ccccacatat aaggtaccaa
    29581 gtccatgacg tcaaatgaaa ttttggctga tgatgcttgg tgtagaagaa cgcaaaaacc
    29641 aaggtcaata ccgtcaaaaa aatttggatc agctgcacat gcttctcgtt ttcaaataac
    29701 tgtaaaaaaa cttcgaatac aatgttgccg actactcctc caacaacagc tccactagat
    29761 acccaaccga caatctgcca attcagtgat ttcccgcttg atacttgtcg cacagtcgaa
    29821 acaatggaca tcgtgaatac cgcaactgtt gagtaaaaag aaatccctgc aacagaatgg
    29881 gcgccgataa gatcaagaat cggtttgatc aacacgcctc cacccatccc ggaaatagcg
    29941 ccaactgtgt tcgccagaac gatcaccaca aaataaatca gaccaatcat cttgtatttc
    30001 ctttctcttg tttatatctt ttagcgattg ttactctttt cttttattat acagtaaaag
    30061 aaacaaacga ggcttcgaaa gcttttctac tttttaagga aatttagtgc taaacgaa
//
LOCUS       DBNICI010000015        29799 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.015, whole genome shotgun sequence.
ACCESSION   DBNICI010000015 DBNICI010000000
VERSION     DBNICI010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 29799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.015"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..2269)
                     /gene="esp"
                     /locus_tag="AC1ZCC_000551"
     CDS             complement(<1..2269)
                     /gene="esp"
                     /locus_tag="AC1ZCC_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HIQ1628830.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
     gene            complement(2887..3321)
                     /locus_tag="AC1ZCC_000552"
     CDS             complement(2887..3321)
                     /locus_tag="AC1ZCC_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7006 family protein"
                     /protein_id="HIQ1628831.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3338..4543)
                     /locus_tag="AC1ZCC_000553"
     CDS             complement(3338..4543)
                     /locus_tag="AC1ZCC_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HIQ1628832.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5237..5623
                     /locus_tag="AC1ZCC_000554"
     CDS             5237..5623
                     /locus_tag="AC1ZCC_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIQ1628833.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5717..5907
                     /locus_tag="AC1ZCC_000555"
                     /pseudo
     CDS             5717..5907
                     /locus_tag="AC1ZCC_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            6421..6753
                     /locus_tag="AC1ZCC_000556"
     CDS             6421..6753
                     /locus_tag="AC1ZCC_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIQ1628834.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            6663..6857
                     /locus_tag="AC1ZCC_000557"
     CDS             6663..6857
                     /locus_tag="AC1ZCC_000557"
                     /inference="COORDINATES: protein motif:HMM:NF024673.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HIQ1628835.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            6896..7042
                     /locus_tag="AC1ZCC_000558"
     CDS             6896..7042
                     /locus_tag="AC1ZCC_000558"
                     /inference="COORDINATES: protein motif:HMM:NF012870.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628836.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            7049..7204
                     /locus_tag="AC1ZCC_000559"
     CDS             7049..7204
                     /locus_tag="AC1ZCC_000559"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HIQ1628837.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            7480..8820
                     /locus_tag="AC1ZCC_000560"
                     /pseudo
     CDS             7480..8820
                     /locus_tag="AC1ZCC_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9061..10677)
                     /locus_tag="AC1ZCC_000561"
     CDS             complement(9061..10677)
                     /locus_tag="AC1ZCC_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIQ1628838.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            10795..11013
                     /locus_tag="AC1ZCC_000562"
     CDS             10795..11013
                     /locus_tag="AC1ZCC_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1628839.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            11208..11669
                     /locus_tag="AC1ZCC_000563"
     CDS             11208..11669
                     /locus_tag="AC1ZCC_000563"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HIQ1628840.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(11862..12101)
                     /locus_tag="AC1ZCC_000564"
     CDS             complement(11862..12101)
                     /locus_tag="AC1ZCC_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1628841.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(12125..13648)
                     /locus_tag="AC1ZCC_000565"
     CDS             complement(12125..13648)
                     /locus_tag="AC1ZCC_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HIQ1628842.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(13666..13788)
                     /locus_tag="AC1ZCC_000566"
     CDS             complement(13666..13788)
                     /locus_tag="AC1ZCC_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HIQ1628843.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(13818..14801)
                     /locus_tag="AC1ZCC_000567"
     CDS             complement(13818..14801)
                     /locus_tag="AC1ZCC_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CobW family GTP-binding protein"
                     /protein_id="HIQ1628844.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(14825..14974)
                     /gene="rpmG"
                     /locus_tag="AC1ZCC_000568"
     CDS             complement(14825..14974)
                     /gene="rpmG"
                     /locus_tag="AC1ZCC_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIQ1628845.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(14995..15372)
                     /locus_tag="AC1ZCC_000569"
     CDS             complement(14995..15372)
                     /locus_tag="AC1ZCC_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628846.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(15404..15583)
                     /gene="rpmF"
                     /locus_tag="AC1ZCC_000570"
     CDS             complement(15404..15583)
                     /gene="rpmF"
                     /locus_tag="AC1ZCC_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIQ1628847.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(15598..15867)
                     /gene="rpsN"
                     /locus_tag="AC1ZCC_000571"
     CDS             complement(15598..15867)
                     /gene="rpsN"
                     /locus_tag="AC1ZCC_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HIQ1628848.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(16390..18132)
                     /locus_tag="AC1ZCC_000572"
     CDS             complement(16390..18132)
                     /locus_tag="AC1ZCC_000572"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1628849.1"
                     /translation="MKSFYKNKFALSDQGAEALTKASISSFFVYCINMVPAFIIMMLI
                     DELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQIADDLSNL
                     PLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLLVGNVKMGL
                     AVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINSFNLSKKVQ
                     DRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTGEVTILYVV
                     GYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLKSFDIELRD
                     VSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDEGCILIDGY
                     DIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARLANCEDFIE
                     KLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAENEKRIQES
                     LNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIYKNLIKKTK
                     LSEKFVYEKEAQSR"
     gene            complement(18116..19870)
                     /locus_tag="AC1ZCC_000573"
     CDS             complement(18116..19870)
                     /locus_tag="AC1ZCC_000573"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1628850.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLIKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(19980..20549)
                     /locus_tag="AC1ZCC_000574"
     CDS             complement(19980..20549)
                     /locus_tag="AC1ZCC_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HIQ1628851.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(20567..21145)
                     /locus_tag="AC1ZCC_000575"
                     /pseudo
     CDS             complement(20567..21145)
                     /locus_tag="AC1ZCC_000575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(21536..21991)
                     /locus_tag="AC1ZCC_000576"
                     /pseudo
     CDS             complement(21536..21991)
                     /locus_tag="AC1ZCC_000576"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(21993..22661)
                     /locus_tag="AC1ZCC_000577"
     CDS             complement(21993..22661)
                     /locus_tag="AC1ZCC_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HIQ1628852.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(22791..23375)
                     /locus_tag="AC1ZCC_000578"
     CDS             complement(22791..23375)
                     /locus_tag="AC1ZCC_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIQ1628853.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(23706..23939)
                     /locus_tag="AC1ZCC_000579"
     CDS             complement(23706..23939)
                     /locus_tag="AC1ZCC_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIQ1628854.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(23954..24904)
                     /locus_tag="AC1ZCC_000580"
     CDS             complement(23954..24904)
                     /locus_tag="AC1ZCC_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HIQ1628855.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(24901..25743)
                     /locus_tag="AC1ZCC_000581"
     CDS             complement(24901..25743)
                     /locus_tag="AC1ZCC_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HIQ1628856.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(25745..26464)
                     /locus_tag="AC1ZCC_000582"
     CDS             complement(25745..26464)
                     /locus_tag="AC1ZCC_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HIQ1628857.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            27012..27314
                     /locus_tag="AC1ZCC_000583"
     CDS             27012..27314
                     /locus_tag="AC1ZCC_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1628858.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            27386..28612
                     /locus_tag="AC1ZCC_000584"
     CDS             27386..28612
                     /locus_tag="AC1ZCC_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIQ1628859.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(28700..29092)
                     /gene="rpsI"
                     /locus_tag="AC1ZCC_000585"
     CDS             complement(28700..29092)
                     /gene="rpsI"
                     /locus_tag="AC1ZCC_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIQ1628860.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(29108..29536)
                     /gene="rplM"
                     /locus_tag="AC1ZCC_000586"
     CDS             complement(29108..29536)
                     /gene="rplM"
                     /locus_tag="AC1ZCC_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIQ1628861.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
BASE COUNT         9768 a         5496 c         4322 g        10213 t
ORIGIN      
        1 aaatatcttt acttacagtt actgctaaat cggtcgtgcc tacagaacca tcttggtaaa
       61 caattttaat aggaattgtt ccagtaatcg gctccgattc tcctacacct ttagctggag
      121 tgaatgtaac aataccagtg tttgcatcaa cacttgcatt accgaagtac ttattataga
      181 catctgaaat tgaaaaggca tctttagcta aaggaacatc tggtttatca aatgtaggtt
      241 ttccactttg aactgtcgtt tctggagttg ttgttatagg tacgtatgtt gcatcatttt
      301 ccatagcttg agaagctttt acagcaaact ctacatccac gtcgaaagtt cgatttccga
      361 tcttaacagt tacaatacct ttcgtactat cagtaacgtt agaagtatca ggctctgttt
      421 tccatgaata ctctgcatct tcaggtaagt ttttattatt ccgaattgat tctttagcat
      481 ctggtttaaa tccaactacc acggtttgtt tatcaaccgc ttttggtgat tccttaataa
      541 cggttgaacc ttcttctggt ttatcaaaac ctggagaaac gatttggatt tttaattcat
      601 ctttcgcgat taatttactt gaatctacac ccgtaaattc aaattctaca gtatctgaaa
      661 ttggagcccc atctttttca tttggagcga tagttttttc tgctaataca gtaccttctt
      721 tatcaacaag ttgggctttg tgacctgttc ccgctaactc gtggatgaat actttcccct
      781 taactgttgt gtcaacatca tatacttctt ctaattcagg tttcccgata tatggattcg
      841 tgtctccgct ctcttcttta tcgtcagggt ttccatcacc atcaatatct tgcaaataat
      901 aataacctgt tccataagtg ttctggatta attttttatt cgtatccgac aaccaagaat
      961 caaagatgaa atcttcacca aatggtttat tttcttcaac ttgtgctgtt gctttcatat
     1021 cttctaagat ttcatctaat ggcttagtca attgtaaaac atatcgaata acgactggac
     1081 gtggacttgc attagcaaaa tcgataagtt tagttaaatc attattagtt attgaaccta
     1141 tgccataatt ttcatgacct tcccgtcgct cgattgctaa atcagctact tttgttccgg
     1201 attcaaacga aacatcatat gttactttgt cagcctgaac catgtgcatc tgtatacccg
     1261 ctacatatgg aactaatcgt tcatcaattt tgtaattagc ataccaaggc gtgccctttg
     1321 cggaagatga atatgagaaa ttataatttt tcgttaactt atgatctaag attatagctc
     1381 cattagcacc tgtggatcct gaaccatctt ttgtttgagt attaggtttc tcatattttg
     1441 cacttactga tccatgttta aactggtcag gattattttc agatacatct aagctattat
     1501 agaaatcatc tgaatctgtt aaaaagtagc ccgtactatc aatacccggt acaattttat
     1561 catcgtcttg atgacttacc aagtagattc gatacattaa acgatcggtc ttgagattgt
     1621 ctatgcctat ctctttaaaa atttcatcta ggctcttttc aaacgtaatt accccattag
     1681 gagcggtttg cgtatcggtt gtttctgccc cagcaaataa tccatcactt gcacgaatat
     1741 aattgacctt ccaaatattt gtaggtctac ccagcgaatc attaattctt acaaaggtcc
     1801 gtctagaaga tccaattcct ttctttggat cgacctgaat tttttctaca tatttagcta
     1861 atcgttcatc taattgaaga cggatttccc agtttttagt tgatgctgca atattatgtt
     1921 ttccgtaaac ttcaaatcca attgtattgc cttttagtgt gtctccagtt agttcttgag
     1981 gactaaattg taaatccgaa aagttataat ttttcgtata accaggattc tcctgaaaag
     2041 tattccccga agctactttg tcacctactt gtacggagct tatcgctgtt tcaagtgggc
     2101 tgcttggaga aatttcatct tgtgctttaa ctagttcagt tgttcccaag acaagaccaa
     2161 caccaatcaa aacagatgca accccaacac ttaatttttt gatactgtac ttcaatactc
     2221 gtttctttgt tttttctaga aatactctct tattattctt gctaaccatt attcaaatca
     2281 actcccttaa taattacttt cctagtaaga ataaaagata aatctgatac gtattattca
     2341 cagaatagga atcttactaa ccttatgtta actaatttac ttagagaatt tcatgaacat
     2401 tacaacaaaa aaaagtacat ttcttcatgt ttttttcgga tatttgtgct ttttaaccat
     2461 tcagcagctg aaaacatata aaaagatatg tatgtattct ttctgaaatt aagttcattt
     2521 ctatgcgatg aagcatgttt tcaaaatatt accctttctt agattaaact tcagagtatt
     2581 caaataacgg aataaggagc cttatcccgc attactgaat gagactgtga cataagtaaa
     2641 aaggtgactt caaattcctt cttcggaatt tgaaatcacc ttctttataa ttataaagta
     2701 tcggtttcac agcatttctg aatgattcct agaatcttaa acgaatgatt ggagttatca
     2761 acattttttc actcgttttt agcgaaacaa aaaatgacat ttgtcacagc ctcattaaat
     2821 tattgattct ttttctttat aattgaattg tccatattta aagtgtctag tactctacat
     2881 gaataattaa ttgctttcac tcaaatactt ataatctatt aacttgtagt tgtttaactt
     2941 ttctgaaaaa gttagaccca cgtaataatc ttcccagctt tctctttcaa ttaactgatc
     3001 caatttttga taatccataa attcatcatg tatcaaaaag aataaataac ttgatatttt
     3061 agcatctagc ttaatcagat taaataatgt ttgtttaaaa ttgctaccat taatatgact
     3121 gatagattct ctagcttctt cacataaact ttttacttta ttaattacta ctttgtcata
     3181 ttcatttaac acactattat taccattact tagacgctca gattctacga aacaactcaa
     3241 atcagaaatt tcccaaaaaa attttttgtt aaaatccaat ttcctcaact cctccaaact
     3301 acgtaaatga tatagtctca taataaaata aaacctctca gctaatgttg ttgaaattgt
     3361 ccactaaacc taattgatat tgcttaggag atacacctgc gtactttttg aatactctcg
     3421 aaaagtgtga agaatctgaa aatcctaaac ctaaagaaac ttcactgata gtatggtttg
     3481 atttcaacat tattttagcc gtagaaattt ttcgcttaga gatataatta taaatcgtaa
     3541 tatgcatttc attcttaaag ctttctctta atttagtttc acttacagaa aaataattag
     3601 ctatttcctt tacagtaatt cttttataga tatttaagcc gatgaattga acgacagaag
     3661 aaattaaggg agaaagatgc tctatcttag tttttccgat ttccttagta taataaacaa
     3721 tcccagaatc cctcactttt aagacctcag gtaacgatac agctaattcg ttttcttgta
     3781 tcaaagcatc tctactttga tacgcttcta ttatatccat tcctaatgtt atagacgttt
     3841 gtgctaactt ttcaaaaaca atgatagaat aatttttttc agaccgaatc gtatctccac
     3901 taacacttgg aattattcca ttactcagat taaaaaccat gttttctaaa tttcgaatat
     3961 ctccattttt aacatattcc agtattttat tttcataaag aaacagatat atttctggat
     4021 cgtaattttg tgataacaga ttatctattt tgatttgctc gatagtttta cttagatttt
     4081 ctgtatattc aattaacggt tcatggaata gatcttctat ttttccagta aaaaaataat
     4141 gaactaaaat cagaatatct cgaatgtctc ctaaagaata caaagtcaaa ttttgcaatg
     4201 aattatagag tcgttctcta tcagccattg caacattcaa attgttcatt tttagtttaa
     4261 aaaaaacttt atcaatatta tttgctctaa atggaccaaa taagtaatat ctggttttaa
     4321 acatgtatag aagaaacatc tcattaaaat caccagtaaa gaaatggaaa tctttagttt
     4381 ttttaatatt atcagttagt aactttttat aatcataaaa aaaataagta gttctatctg
     4441 agcgatactc ttttataata ctgaattcgg aattaaaaac cacgataggt aactttaaac
     4501 aaacatgaag agactttaat aagacttctt tatctgaaaa cacttttgtt tctctctttc
     4561 ctttctacat cattaaatag tattttatac gttatctaaa atactattcc tctgtcaaaa
     4621 taagctaact tacttcatta taaaatatcc acttatcgct ttcatgtata ttccaccgta
     4681 aaaagtacat tttatcaccg agaactcatc atttttcatc atgatgaata ctatttacat
     4741 atatctagtt caaagacaat aattattatg atccgttatt atctaagcac tatacagata
     4801 ataggaattt ttcgtttcta aatattccaa tttgggaatc caataaaaat tttcgttcaa
     4861 atacgtgtct attcgaactc ttttctagtt gttaattatg aagggggaag aaataaaaaa
     4921 tacaatctat acacgaataa gaatattatc caaaaggagg ttttagaaaa gatttattat
     4981 acgtaaaaag ctcaagacat tttcttagcc ataaggacat gtttgatatt taagaagata
     5041 tatgaatttt cttttctaag tataaagata actatccatt atcaactaaa aaaacatttt
     5101 tgaaaataaa catcagcagt cctatcaatg catgtgagct tatagaagtt attcgcagcg
     5161 cagcataatt gcattagctt cttttcgtgt tttttgcctc ttttcccttt aaatcttaat
     5221 ggaggaggtg tgcaatatgt cagataacga cgaacgcgta aatgcccagt tgattcgatt
     5281 ctttgaaaag gtcattttga acaccgctaa tacttattat aaaaaacaag aagaaattca
     5341 aaaacatgaa caacttgatc aactcccacc atatttgttt actaaagata ctataaaatt
     5401 taaacaaccg attaatgttt taaatgtgac tctccttgtt gaagatataa aattagcaaa
     5461 aatccttcct ttgttgaaag aacctgaaca aatttttctt atcgaaaaat ttatctttga
     5521 taaaactgat aaagaaattg gaaaggttct ggggattaca agacagggtg cgacaaattt
     5581 aaaacatcga ctttacaaaa aaattcaaaa aatgctggtt taatatctgc ataataacta
     5641 attttgtatg cttctttgct cgttctcctc cttcccaaag aaatagatta taagtccatg
     5701 ggaagaaggt agatttatga attctttaaa agaagatttc attttagcaa aggctggaaa
     5761 tgaagaagct gttgaggcaa ttctcaaaag atttagctct ttgattcata agcaatcctg
     5821 agaactggaa aatatgatca agattgttat caagaatgta tgcttgcaat atatctagct
     5881 atctcgaaat tcgaaattaa agaataactc aactaaggga gtctgtgaca taagtaaaaa
     5941 gatagtttca aatttcgaac aataaatttg aaactatctt ctttataatt ataaagtatc
     6001 gattttacag cgttctataa tgattcatag aatctaaaac gaatgatttt aattatcaac
     6061 gttttttaat tcgtttttag cttaaaaata acttttgtca cagtctcact cctaaaatta
     6121 ctttatttac gtatgaagtg ggcaggaggt cgaatccaga actgggctat ggtacttcaa
     6181 cgaggacttc tcctctagaa ttgaaaagca tgcgatttac cttccgtaac gacggtagat
     6241 ttattcaact atgtgtccaa tcaaaatttg tctgatttca aaaatagtca agagaatttt
     6301 ggttgttaaa aactcttatc tatcaacttc cacagtttag ttgacaaacc ctgatactaa
     6361 gttgggctat gatacacttt tcttgactat cagttacgtt accggaagga atgaactcta
     6421 ttgagaaaaa catatgatcg taaatttaaa ctaaagattt cacaggatat ccttgagaaa
     6481 aaagtaacta ccaagaaaat tgctgaagaa tacaatatct ctcgtccaac aatttcccga
     6541 tgggtttcag aatatcgtcg atacgggaaa aatgcttttg ctggacaagg aaaaagatta
     6601 cctgataaag cagatttcta cattcttgaa caggaaaata aacgactaaa agaggaagac
     6661 gagtgttcta cgaacacaaa gatcgatacg gatctccagg gatcactcaa aaactttacg
     6721 atgaaggaat cgaaatcaat aaacgtgttg tagctgttct tatgcgtgaa atgaatttat
     6781 gcacaagagg atttcatcga cgcaaatctt ctcatggtag agaaaaaaca atcaaagaaa
     6841 tggtaaaaaa atgttgaatc gataatttga acagaaccaa attgatgctg tttgggtgat
     6901 ggatattact tacattccat gtaatgacgg tcgcctctat ttgtccacct atattgactt
     6961 agcaactcgt attctacgtt actatatggt agattcacac atgaaaaaag agattgtcgt
     7021 ccgtccatta caaatttatt aaggagaatt gcctacggta attcattccg atagaggtag
     7081 ccagcatcgg tcttttattt atcaagagct gttgaacgac caccacatca cacattcagt
     7141 gagccaaact gggactcctg ttgacaatgc agtaattgaa gcgtttcatc gatcaatcaa
     7201 atgagaatta atcgactcca acaaatataa aagtaaaata gagatgaagg tgttaatcca
     7261 agattattta gaagactact acccaaacaa aagaatccat acaaaattta tgatgactcc
     7321 ttgacaattt gaagcgaaga aaataaggga tctataatat ctagtgttgg tgacgccttc
     7381 tgttgccaat actttttctt ttttctaata aacgcaaaca gacaccttaa aatcctttag
     7441 aataaagtcg acgaaaacca ttcaaaggag tgtttcaaga tgtcctattc agagtttacc
     7501 aaagagttac tagatattct agacctaaat cttatctttc atgaagatgc ctttcgcaaa
     7561 gagcgaatca atgacgaaac atgttttgtt tttgatggca cattgaccta tcaaccggag
     7621 gaatgctttc atcgccacta tcaaaatgaa caaacgatca ttaaataggg ctggaaaaaa
     7681 gtatccatct tattgaatga tgtcagtaac tacaagacca ttctgcggat taacaaacag
     7741 cgcttcaaat gtaaacactg tggaaagact tttttagcag aagattcagt tagtgatcgt
     7801 cgctgctcca ttgctcgcag agtcaaacaa gccattcttg agctattaag cgaacctctt
     7861 tctatgtctt tgatcgcacg tatgaagcac atttctccga ccactgtcat tcgaatactt
     7921 cgcagccttc ggcctaaaac catttctttg agccagctgt taccagaagt ggtgtgtttc
     7981 gatgaattca aatcagtgaa aaatgtctcg ggagccatga gctttgtcat gatggacggc
     8041 aagacccatc aactgattga tattgtggaa aatcggcaac tcaatccttt acgcgactat
     8101 ttcttgcgct accctataaa agtccgtgaa caagtgcgtc tagtggtttc agatttctat
     8161 acaccctatc gcacattggt gaaagagtgt tttccgaatg cccgaatcgt cgccgatcgc
     8221 tttcatatta gccaacacat tggtcgggct tttaccaacc accgaattca agtaatgaag
     8281 acctttaaaa aaggggatcg tcggtcgaag cacttaaaga aatactggaa actacttcaa
     8341 aagaatgcgt gggaattgaa aggtcagcat cgctactggc gtccgtcatt tcgagatcat
     8401 ctgacagaag cagagatagt cgatcggcta ttatcttatg atgattcctt aaagcgaggc
     8461 tacgaagtct accaggattt tctttccgtt atcagaagac aagatgttcc tgaattcgtc
     8521 gcgctcctaa aagaagacta caaggagttg ccagagcact accaaccagt gttcacgacc
     8581 ttcaagaaat accaaacaga aatcaaacga gcgttgcgcg ttccctattc caacggccct
     8641 atagaatgtt tgaataacca tatcaaagta ctaaaacgaa ttgcctatgg ctatagaaac
     8701 tttcaaaact accgagaacg aatcttttta tatcgaggaa aatattttaa gaaaactaag
     8761 aatactaccc aactgactaa agccagaaca acaacacggc tagacaagat agctgtctag
     8821 ccgtgtgagt atctcgatta ttctattgaa tttcttcacc aacactcatt gacgaagagc
     8881 caaaataagt aatctaaccg actgttaact aaaccattgc aggtcccgtt aaagatactt
     8941 gtgcctgttg tcaccgcttc tttttcttgt ccacaagcgc ttaacacact taataaagcc
     9001 cctgctatta ttaataataa tatctttttc ttattaaaaa cctcctcatc tatttccaga
     9061 ttagtaaatg tagtaatctg tgctttcttt tttctcaata ctaataacta acttatgggg
     9121 taaacaaata ctcgtttgac cgggttttga gatccatcca gttctaaccg cgatttgatc
     9181 aggactatta tcttctttaa ctcgaattct tccatcttta atctcaacaa tattatattg
     9241 tcccttcgca ggatgataat tcacgagttt atgattgatc ttatccaaat cgaagcgatc
     9301 catttccttg ccatcaattc gaacgatggc atatttgata gcatcttcag ttgtttcatt
     9361 ctccttttct atagagaata caaataaggg aataaaagag gtaataatca aaatgccaat
     9421 aattatgaca tcgcctatct tgacaccagc ttttcttaaa aatttcatta acgatatctg
     9481 gctaaacata ttagactttt tccatggaaa tggtgaaatt tttcggaaat tatctgcatt
     9541 aagagaatca gttaaacaac cctcaagttg ttccttaata cattgttgat ccatcttcat
     9601 accaataaaa actagtttat taatgcgttc accaaacaac ggatgccagg cttccttcaa
     9661 ttcgggattt ttttgtataa tcatttgaca ctcaatttta ggtaaactag ctacccaata
     9721 atttaatgat tcaatacttt tatagctacc cgatgtttca aagatcgtcg aaaaatcact
     9781 atgttgggca aaccaaagaa acccttttgc tcttgtaata ttgccaggaa aatttttatc
     9841 cgtaagatgg gcaaattttt gtgcgtcaag gagtaaccga tttcgataga taaaacttga
     9901 gataccatat tcttctgttt caggaatatg attttgatag ccaagctcta actctttgaa
     9961 ccagcccact gaataggaag tctcatctat attaaaaaga tttgtattta gaattttcga
    10021 taagtctact tcacctttaa ttgtcctaat tattttggca ttggactgta acttagcaat
    10081 tagaccgtta attttgtaaa tcgtcttggt tgacactaag tcactattat ttaaaagtaa
    10141 tatgttacaa cactcgaatt gatcaattaa caagccacga atatcctgtt cttcttcaac
    10201 tacattacct tcagcttttc tttcctgaat cttttcaccc gtttcgaaat tatcccaaaa
    10261 acgttttaca tccactacta taataatggc accaatctct aatcgttggg tcaaatcaat
    10321 gtctaaaggg tttttagcta atataattgc ttgaataatt ggaattggtt tgctgattcc
    10381 tgaagcttca ataacaattt gattcaagtc agaaaattgc actaattgat ctagttcaac
    10441 aatcaaatct tcgcgtaatg tacaacaaat gcatccattc ggcatcgaaa caagtttttc
    10501 tccagttctt ttaaaacctt tcttgtcgac taatacccca tcaacattta cttcccctat
    10561 gtcatttaca atgacagcaa tttcctttcc gtgatcaccg tacagaatat gatttagaag
    10621 tatcgttttt cctgaaccaa gatagccagt taagattgta ataggtattt ttttcatagg
    10681 gaacctcttt ctataccttg aatttacact ttaagtgcaa cacataaaaa tgagtcataa
    10741 tcgatatcca actagtataa gtttgtcgag aacactccta gaaggaaaat aattatgagc
    10801 cagtatcgtc atcttactat actcgaacga gaaagaatct ttctgcttca cgaagaagga
    10861 ttttctatta gtataattgg caacgacatt cagcatagcc cgtttactat tttacatgaa
    10921 ctgagaagaa acaagaaaaa aactattcac cttccaaagc ggaaaaaaat tatgcttatc
    10981 gaagaaaaaa atgcggacgt tatcacattc taaatgatcc gtgagtttgg tcaattgttc
    11041 gtcatttatt tgtcgatttt cagtggtcgc cagaggaaat ctcgaatcgt ttgaagcatg
    11101 aacatatgga cttttcaatt agttttatca cgatctaacg tgcaatttgc aatgatcttt
    11161 tcgatacaga acccctctca cacggcaata aaggcctgat ccgttcattg caccatcgag
    11221 gaaaaacaag acatacaaaa aatcatgtcg agtctcgtgg aaagatttgc cttactcatt
    11281 cgatccatgg ccgtccaaaa tccgcagatg atagaagtga ttttggtcat tggaaagccg
    11341 atattgtagc tggcaaaact ggtggtactt gtttagtgac tttgacagac agaaaatcat
    11401 gcttcctttt agcaggaaag aaggtcccca gaaagaaagc cgcttatgtg agcgagaaaa
    11461 tgattgaact tttaagcagt attccaaatg aggcggtcaa aacgatcact ccagatagag
    11521 gaaaagaatt ctccaatcat ttcgcagtta cagaagcatt gaataatatg cccttttatt
    11581 ttccagatcc tcatgccctt ggcaacaatg aacaaattaa aatacgaata gactcattcg
    11641 agaatacttg ccaaaatcga aagagatagc tacggtagat aactcaacga taaatcagat
    11701 gattgaaaaa ctgaataaca gacctagaaa gtgtttagat tggagaacac cttatgaagt
    11761 cttctttgat aaagtgttgc gcttgatttg acaattcaag acataataaa aagcagatca
    11821 aatccaaata agggaaaaat gatctactct ggtattatca attaattggc caagctatta
    11881 tgccgttttg ctaaacgtct acttcttcca gtattgacct tgataaaaca acaatcagga
    11941 tcccgtttta atactctatg aactgtgtca atactaacat gtaaagttgc agcaatatca
    12001 gctaatgtac tcccatttaa tctcatttgc tttgcaaatc gataacgccg ataagcatca
    12061 atattcttgg aatccataga caataatact tttcttacca tgtatttcct ccaaaaattg
    12121 aattttaatg agccatcatt tcttgagcaa tttcatgttt agacaatgaa gctggataga
    12181 aagtaggcca attatgcaat tcattataga gtttttcttg actttctcca ccaaagaaaa
    12241 tatggaaatg ctctgcctta cttggtgcaa ttccatgatc actaaattga atatatttaa
    12301 aacgaccagc acctgcatca tcagtttcaa aattaaaacg aacaccacgg tttccttttt
    12361 cataattcaa aattttgtat ccaacatatt tgtatgtgta ttgatgatgt tcaccgttga
    12421 ctacaaaatc aatggtgttg tctttaatgt tgatagtatc aatatctgtc ttatagccag
    12481 tagtataata atctttgtac tcggcagctg tcatgtcttt attgattttt gctttgtaat
    12541 cgaaaacttc atccaaaatt ccatctttta acaacggata aacagattgc cattcgcctg
    12601 tataatcact caattcgcga tccttcacat cggcatcttt aaagtaccca ttagcaactg
    12661 tcttttcatc cttcgcttct ttttctggaa ctacctcttc accagcaacc atagttgttt
    12721 tttctaacgc ttttagattg tcttccatta ctgaaacata atcttcacca ttatccattt
    12781 gttcttttgt aagactttca agaggattca gtacttccaa ttttatccca gcttcgttgg
    12841 ccaaagtttt tgcaatttta tcattagcat tcttttcaaa gtaaatataa ttgatcttat
    12901 tttttttaac atactctttt aattcagcta atcgaccaga agatggctct tcttcaggac
    12961 ttagtccagc aataggaact tgtttcaatc cataatccaa agctaaatat ccaaaggctg
    13021 cgtgttgcgt cacaaaactt ttttgtttcg cttctttaag actagtggta tagtcagcat
    13081 ccaaccgttt cagtttagtc aaatactttt cagcatttgt ctcaaatact tttgcttttt
    13141 tcggatacaa tttaactaac tgatccttga tatttgaaac ttccttaatt gccattttgg
    13201 gtgaaaccca cgtatgagga tctagttcat gatggtgatc ttcttctccg tgatcatgat
    13261 cttcttcttc actacctggc aataaaatca tgtcttttgt tccttctact acattaggcc
    13321 cagctttctt ccaactttct ttagcttttg gtacccatga ttccatgttt tcgttatgat
    13381 atacaaaaac atcagcatca tgagcttttg ccatatcctt agctgagggc tcatagtcat
    13441 gaggttctga tcccgctgga accattaaat ccacgttcgc ttcatcgccg acgatgtttt
    13501 tggtgaaatc atacattgga tagaatgttg ttacaatttc taattttcca tctttatcat
    13561 tcgatacttt tgcatcatta gtgctggagc aagctcccaa taatcctaga gctgctatcc
    13621 caataagtcc tacactgatt ttcttcattc tttttccttc cttaattatg ctaatttctt
    13681 cattttattt cgtttatgtt ctttgataat ttttaatgcc ctttttctag ttttaatatt
    13741 cgggctttta agtcgtcgat atgcactaga tatatccgtt ttctccatta gaaattaccc
    13801 ccaatcacta cttatcatta tttgctttcc gccagtaatt tatcaaaact ctgtttgatc
    13861 ctttcttcat ccaacatttt tccgattaga ataatagtgg tttggtcttt ttgtcgagtg
    13921 gtgtattgaa aattaacctg ttcgtttacc tcttgcatgg caactataaa atcctgctct
    13981 tgtaaagcaa tcaatccttt aaaacgatac aggctccctt ggttggttaa aatcagccag
    14041 tcaatccatt ggagtaaaaa atcttttttt atgtcttgat ctgaagttaa gagaattgat
    14101 ttaaactcat gtacttgatg gtgttgatga tagtatttgc cattatagtg attttctgat
    14161 gaatcacttt caaaaactaa gggatgatta aattttttca aattataaaa atcctttttt
    14221 tgaatcgttt cactatgtgt gaacattcga atatcggata aagggttaat acttttgatt
    14281 tctctctgta atttttttag tgcagataaa ttcaagtttt gttttctaga cacaaaaatg
    14341 cgatctgcca tgacaagttg cttaagggct tgtggttctc tttcaataag ttcaaaactg
    14401 tcactatcaa taacagtcaa taaactatcg atatatactc ttcctcttag ttgaggcgct
    14461 gttaaaagtg tttgaatgat tggttgggga tctgctatac ctgtagtttc aatgatgatt
    14521 tgtgaaattg gttgttgatg ttcttctatt gtttccagta aagaaatcaa tattgctaat
    14581 aaatcactgc gaagactgca acacatacat ccattattta gttgtaacac ccgttcctcg
    14641 ctatgaacta ataatttgtg atcaatccca acttcaccaa actcattttc aattataata
    14701 atttcttccc tggggtaggg tgagacttgc aaggcttgat tgattagcgt tgtttttcca
    14761 gcccctaaaa acccagaaac gattgtaact ggtatgccca ttttgtcacc tttttaccac
    14821 ttagctattt cacttcttta aaaattgccc gtctgcgcaa ttttggcgaa tactttttta
    14881 attctaaccg ttcagggtta ttccgtttat ttttgctggt gagatacaga cgttctccag
    14941 tttcgacaca ttccaaaata atattttgac gcataaatct tcctccttca atacttatcg
    15001 atcttcaaga tatgcttgaa tttgttgtgc atcttcaaaa gttaactcgc cagattccaa
    15061 ttgtgaaact aaattttctt tcatatcaac aggtatctcc caataaccat cgatacattg
    15121 aaataactcc tcgtcatact cttcaaaaat tggatagatt cttaaaaaat catcaaatga
    15181 taagccttgg tcctcatagt aatctttgac taatccattt tcaattgcct gttgggtcgg
    15241 taaacccgtt tcagtaataa ttcctctctc taccatcgaa cgataaaaag tgcatttatc
    15301 tagcgattgc tgggtcgttt caagataata ctttaatcct tcttcaacta aaggtgggta
    15361 aataatttcc attttgattc cctcctaaaa gttatatatt aaactatttt gaagctcctt
    15421 taattacttt ctttccatta tagtaccctt ttaaagatac atgatggcta cgtctataat
    15481 caccattcgc ttcgtcaaat gaaatttctg gttttactac agtttgatga gttcttctta
    15541 accgtttctt cgccttagat gtttttcttg ctggtactgc catgatattt cctcctttta
    15601 ccaacttgct ttttttactc caggaatcaa cccttgatgg gcaagttctc taaacttaat
    15661 acgggacata ccgaactttc gcatataccc tcttggacga ccatccgtct gatcacgaag
    15721 cctcagacga ttcggatttg aatctcttgg caatttagcg agagcggtgc gatctcctgc
    15781 ggcttttaat tcctgacgtt tatctgcata cttttcaatc atcgccattt gtttctttgc
    15841 tttagcaatt ttcgactttt ttgccatata cctttatact ccttttattt tgatttagac
    15901 acctcaaatc gtaatcgtta cgttttgaca ctgatacttt accaacaaga gttgctcttg
    15961 tcaacaataa ataacatgaa ataaaactca tatgaataga taactatttg aaaacgaatt
    16021 tatcggtaca tagagataat caaatcattt aaaagaatat gcagtttagg ctctgaaaaa
    16081 taagaattca cttttattag tccaatcaaa cacaaatacc gttttaagta gcttttcctc
    16141 tacttaaaac ggcaatatga aagaaactat aactttttat tcaatctttt atgtttaact
    16201 ggtttatcgc caatttttta aaactttgat tattccttat attataagaa tagacattaa
    16261 gtgaactaat tcaatcatac tgattctttc gtgtattcaa aattagatgc tgtttaataa
    16321 aattattctt tttgaaaaag aattataact atatcccaaa aattgtaggt taatttaaag
    16381 cctatttcat catcgtgatt gagcttcttt ttcatagacg aatttctcag atagtttagt
    16441 tttctttatg agatttttgt atatttctga ccttcgatac aactcattat gagtaccttg
    16501 atcaactagt tttccttgat ccatcactac aattttattt accttttcaa tagacttcaa
    16561 gcgatgcgat ataattagta ctgtcttatc ttgtattaaa cgatttaaac tttcctgaat
    16621 acgtttttca ttttccgcat ctaaactggc agtaatttca tctaaaatta aaattggcgc
    16681 atttttcaaa aaagcccgtg ctatggatag gcgttgtcgc tctcctccag aaagtgaact
    16741 tccgttttct ccgaccaatg tatgataacc gtcaggtagt ttttcaataa agtcttcgca
    16801 gttagctaaa cgagctgctt ttttcacttc ctcatctgac gcttgactat ttccaatacg
    16861 tatattttcc aaaatacttg tattaaacaa agttacctct tgaaaaacaa tagcgatttt
    16921 gctaaacaaa gaagctggag atacacgttt aatatcatac ccatcaatta aaatacagcc
    16981 ttcatcataa tcatacagtc tagacactaa tttcaagata ctagttttcc cggaaccact
    17041 agcacctact aacgcagtca cttctccttg tttagccgta aaagagatat gatctaaaat
    17101 tggggtattg ttatcatatg agaaagagac atctcgcaac tcaatgtcaa aagatttcag
    17161 aggactatct gatccctctt ggatactggt ttccttcata gctctaattc gctgtatttt
    17221 aggagctaaa taaaatattt caagcactgc ttctttcata gaatcgaagg aatcctttat
    17281 ttttattgcg gctagtaagt aaccaacgac gtagagtata gtcacttcac ccgttaacag
    17341 taagtgaaca cctactagaa tgactactgc taaactaaca tatgagaaaa tagaggataa
    17401 ggccatgact gaaaaagtac ttaattctac ctttaaatga atcctttcac tctcttccat
    17461 ttttttgaaa agtctgtctt gaactttttt agatagatta aagctattaa tctcttgctg
    17521 caattctata gtttcttgaa attcttccga gttttctctc aaagtatcgt aatatttagt
    17581 attggctttc gtttgggatt tctttgataa caagattaag acaaaactaa ataacgttgg
    17641 caaaataaca gctaatccca ttttgacatt accaaccaaa agcatcactg aaataaaagg
    17701 gaaaaacaga gccataccgc cggctttagg tattgcatga ctcatcgcat gctcaatacc
    17761 ttcaacgtca gacatgatag tttgagataa atctgataaa ttatgttttg aaaaatagga
    17821 taatggtaaa tttgacaaat cgtctgcaat ttgcactctt aaatgcgcac tttctttata
    17881 agtactatta tataagtttt catactccct atctaacaac caatacatga aaagcaaagt
    17941 aacaaacgat acggcaaaat aaagccaacg gggctttgta ttttctaaaa ctaattcgtc
    18001 tatcagcatc attataatga atgctggtac catattaatg cagtatacaa aaaaactaga
    18061 aatagaagct ttagttaaag cttctgcgcc ctgatcactt aaagcaaatt tgtttttata
    18121 aaaacttttc attgtcaagc ctccaatcat ttgcattctc ataattagtg accaattgtt
    18181 tatacacttg atgtttagcc attaattcat tatgatttcc acgttctact attttccctt
    18241 ctgacaagac aagaatttca tctacagatt taatgctgga taatcgatga gctatcataa
    18301 taactgtttt ttcttttatc agatgttgaa aagccttttg tagttcatac tcattatctg
    18361 gatcaataga tgcactggct tcatccataa taacaatctt tgcatctttt aaaattgctc
    18421 tggcaatagc tatacgttgt ttttctcctc ctgacagata aactccttta ctaccaatta
    18481 aggtatgctc tctttcggga aatttttcta aaatcgattc gcaaccggct aatcttagcg
    18541 cgtcaaatac tttacttgct ggcgcttgag gatttgctat agaaacgtta tcgtaaatac
    18601 tctgattgaa tagtttcgta tcttgaaaga caaaagcaat tgtgttaata attgtgtctt
    18661 ttgaatagtg atttaaaggc ttaccaccaa ttttaatctg tcccttatcc acattgtaga
    18721 aacctgaaat tagtttagct atcgtactct ttccgcttcc tgattttcct actaatgcat
    18781 aaattctttt ttgttctaaa actaagttga aattttctaa aacacactct tctttgtatg
    18841 aaaagctgac attttcaaat gcaatgtcac aatttttaat ctgtgtctcg ttcccatatt
    18901 ttagttggtc ttgcttcatt tctaaataaa gggagtctag tgtttcgact gcatatcttg
    18961 cttcaaacaa gtgcatactt acatacatta ttctcatgaa agaaacaaaa ataattccac
    19021 ttaaaaaaaa caacatcaac aagtcaacag ctaatatctt gggagtggta agagaagtta
    19081 taaaaaaggt tataggaact atcaaaatgc aaactatgcc aaaaaatagc cattgaaaaa
    19141 gaacaaaagg tgttttccca ctcaaagaat acttatatgc aaacttagca taatcgttaa
    19201 ttgctttatt caaggcttta aatgatgtta ctttagcacc aaatatcttt ataacttgaa
    19261 ttccgcggat atattctacc gtttcagagc ttaacacatc taacgattct tgatattttt
    19321 gaataaaatt tgtattctct gtcattttct ttaacaagaa tacaccaatt actaacatgg
    19381 cgattagaac caatccaatt ttaaagttaa tgaaaaagcc taaggcaact gataaaacag
    19441 gtgttactat ggcttgagca ctatctggaa tcaagtgagc aattgccata tgtgttttag
    19501 ccgcgttgtc atcaattgtc tttcgaatat atcccgaaga atttaaatca aaaaaacgaa
    19561 aacttgcttc tgttaaaccg ctaattccct tttttctcaa attagtctct aagcgaaacc
    19621 caaggatatg agaaaataag ccagaaaaaa tataaaataa agctcctaaa gttaaccaag
    19681 caacagtttg aaccgcataa ctcttagcta aatattcatc tcctacaata attagagact
    19741 gaagaaattt gtaaatactg taatatccat agactgttaa tacgacagaa aaatcagaaa
    19801 ataaagtggc taaccatccg tagtatttca ttttagggac ataactaaat aattttttgt
    19861 atactttcat tttttcctcc cccgcattca atttcttctc ttattcgttt acaactgtta
    19921 atgctttccg taatagcgta aattagagat gtacaaacag ataaaaggta aactacgttc
    19981 tattttttgt atttttttaa gacttttctt ccaatcaata caccaatcat agataaactt
    20041 gctacaacta acactgaaat gattccccat atacctaccg catagtggat cattgcatcc
    20101 attgttgcag cagattgtcc tttatctatc caatcttgac gaaaacgatc tgtaaagaac
    20161 catataggaa tccactgtcc ggcagcactg cctaattgca ttagagccca agacgcaact
    20221 tgaggaattg ctcgatcata tccaaataca gatagaataa tatcagctaa gatcgccata
    20281 ctaatcgaac tgataaacca aggtaagtac gccccgccca ttaacgtaaa aaataacaat
    20341 aaaacaattg tgtaaatagt aaaaattcct tttctagggt agttatagct aatataaaca
    20401 aatatgattc cagataataa tccaaaaatc ccgggggata tgctgtgacc aaacgcccca
    20461 ccaatactac taaccattat tgaaaaaaag taaattacta gggaaataac tgtagctaaa
    20521 ccaataaaaa tataatcttt tgttttcata tatattctcc tcaattctat ttcgttatgt
    20581 cattaaaaat ctgttctatc aaagttgaat cttctactct aagttttata tcttttttta
    20641 ttccaccatt ttttaaataa ataattctat tgcatgtttt ataaagaaat tccggatcat
    20701 gagtagctat aactactgca cccttttctt ttaagtcttt aataaggtca gcaatccttt
    20761 ccatattaat ataatccaac ccacttgtag gttcatcaaa tagaaataaa ttagtttttg
    20821 acaatgaagc tatagaaacc aataatcttt gtttttctcc accggataaa tcaaacggta
    20881 attgatcttt cattttataa agttccattt tttttaaaat tttttcttgc tgatctttac
    20941 taactatttc attccccagg gctaactcgc tcctcactga ttctgtgaaa aattggtaat
    21001 ccatttcttg cattactaaa aacggcgatg acaaattttt tttattttcg attatctttc
    21061 ctttggtagg gcgaataatc ttcatgagta ttcttagtaa ggttgttttc cccacaccgt
    21121 ttttccctac caaaccaata acatctccaa ctttgacatc aaaatttatg tcttttagaa
    21181 tattttcata agtaatttct tcaactttta atacatctgc acttaccttt tcgatatagg
    21241 gaacttcaat cgaactatta aaaatgtcaa atttccttaa tgcgttattg ttttcagttt
    21301 ttaacaactg aatagttgta gtttttacag ttctattttt aacaaataga acttgatcaa
    21361 ttaattcttt caaatagtaa aaacgatgtt cagctataac aatagtaaat cctttatttt
    21421 ttaaatcaca aagtaagtca gcaagtttct tagtcgcttt gaagtccaaa tttgaagacg
    21481 gttcatccaa tataattaat ttagagttta gcgataaact acatccaata gaaattttct
    21541 gccgttcacc acttgatagc gaaaaaatgt ttttatctaa tatatttgaa agatctaata
    21601 attcagctaa ttcattgatt cttttatcca tatgctcttt tgtaaatcca aagttttcca
    21661 tcgaaaatac taactcactc gttgtatttg ttgtaaagaa ttgtcctcta ggatcttgac
    21721 ttacgtagcc aatatttttt gatatacttt cttgtgttaa ggattgtagc gagttatttt
    21781 caaataaaat ttccccaacc aattttccgt cataaagtct tggaataagt ccattcatta
    21841 actttaatag tgtggatttc ccactcccag aagaaccagt aatcaatgta agactgcctt
    21901 tttttattga aaaattaagg tcaacaagcg cttgttcctt aacatcctta tatgaaaaag
    21961 aaacattgtt aaattcaaac aattctttca cctcaaatca aaatacttaa gaaaaaaata
    22021 gtaacggaaa acaggatgat atagtctaat aagctcatgt ttgttttata gtaagaactt
    22081 cttttcacag gagattctat cccctttgtt attgcattag cggtcatttc ttcagcaatc
    22141 atagttgcac gtataagaac gggaactaac agaaattcca aatattgcag aggctgaaaa
    22201 aaagacaagc gaatgtttct catttttaaa gattctttaa tcattcttat ttcagaacga
    22261 taagtcggaa agaatctgat cactactgta atacttaaca ttattttttt cggtaaatgt
    22321 attttttcta aagaggataa taattcattg ctagtataga cactaaccaa caaccatgag
    22381 atcattacga ccggcaacat tcgaaataca atatataagg atgtcgcaaa aaatatagta
    22441 aaaaactcta tcttgatagc atcaataatt ttcattacta aaaacagaac tgaataaatc
    22501 aagatgaaat aaatacatgt ctttatttta cgaaacatca atactaaaac tgtagcaata
    22561 atgaaacacc ccacttgaaa tatatttccc tttcctaaaa aaataatgac cggaaaaaaa
    22621 atatttaaag taatcaaggt taacggattg atttttgaca ctctttcacc tccaaaaaat
    22681 aagatcgcac tgttatctta agctgataaa aatgcaccgt tattcagata acagtgcgat
    22741 cttatcaaaa agcataactg agaacaattc tcaatgcaag ctttttttaa ttaactcagt
    22801 aattcaatcc acccaggaac agaaaattga tacaatcttc tggttaatag catagtcttt
    22861 tcttttgaat agctatgctt caatatatca tagaatgtag cgtagaatga gtaaatgtag
    22921 aagtggattt cttcttcatt aaagactaaa ttttgattga tcattccgat agtctttaac
    22981 ttatctagac aacagactgt ctttttcgtc acaaatgatg taattttttc aacaatatct
    23041 tgatatttgg ttccagctga ttggaataaa atcaaatcga atatacgttt attctcaaaa
    23101 atataattaa taaatggatc taattcattg aagttctctc tccaactttt ctctatatct
    23161 atattttcta agtttaagag gtatttggta aactcttcat tatacatatt aataagcgca
    23221 ttaatatgcg gctgaataat ttcttcaaat aacgcttcct tagaagtaaa atgtttataa
    23281 aaagcacccg tagttacgcc tgcggtcagg cacagttgcc ttatcttagt ttgctgaaat
    23341 ccttcttttt caaaatggct tagtccactt tctattaact tttggtgagt tataatataa
    23401 tctctggttt ctatgggtac ttcctcctaa taagatgaat taactttatt atcttacaca
    23461 ggacttgaag tttcaatcgt taacgtttat tactgaactc attccttaga gctaaactaa
    23521 tgagaaagag cagaataact gatacaaaaa gcaatcctac aagaattaat agactcatct
    23581 aaatcattca ccttttctaa aataatccga gaaagattta aaaaaattat atccagctat
    23641 taggataagt cctatcataa tcactccaat agccattttc ttatcctccc taaaaataaa
    23701 gtcttttact taaaaccttt tttgtaatcg tcaataaatt ttttcccttt atcagttaat
    23761 tgaaatccta tatctaaata gtcctcatat ctaagtttag ccagcatctc acttacagat
    23821 gctggactaa cacttagaac ttttgctaaa tcattatttt ttaatttctt ttttcttttt
    23881 gataattcct gaattgctag taaataaatt agtatagtag tcgttttaat ttgtaccata
    23941 cctaccctcc aaattatttc gaaaggcctt cagcaatttt atccaggttc catttcatca
    24001 tcgcatagta actatcacca tcttgtcctt tttctgcaat tgaatcagta aaaatcgttg
    24061 aatagatagg aatattggta tcttttgata ctgttttcat tggtctatca tccacactgc
    24121 tttctacgaa taaggaggga acttttgttg tgcgtaattt ttcaactaag tgttttattt
    24181 gatctggtgt tccttcttct tcagtgttga tttcccaaat gtaagcagaa ggcacattat
    24241 acgctttcga gaaatattta aagcatcctt cacttgtaac aatcattttt ttatcattcg
    24301 gaattgaagc aaatttagat ttagcttctt tgtctagaga atccaatttt tcaatatact
    24361 tatctagatt ttctttatag aattttttat tatcaggatc tttttccgct aactgttttt
    24421 caatattttt agcgtaaata ataccgtttt ctaaatttaa ccaagcatga ggatcttcct
    24481 tccctttctc actctgaccc tctaagtaaa taacatctat gccatcactt gctgcaaaat
    24541 agtctttgtt ttcctctttg ttcgcatttt taactaattt tgtaaaccaa gcatttcctc
    24601 cagtttccaa gttaacaccg ttataaaaaa tcaaatctgc ttttgaagtt ttttgaacat
    24661 cttcaggcaa aggttcatat tcgtggggat ctttcccaat aggtacgata ctgtgtaaat
    24721 caattttatc ttttgctata ttttcggtaa tatctgctaa aatcgaatta gtaactacta
    24781 cttttaattt ttcgttcgaa gccactttat ctttgtcttt actatttatt gaacatgccc
    24841 cgaatataaa aagagcaatc aaaatcccca gacttcccag tactgctttc cattttctca
    24901 tcataacgat ctctccttct ttcttaaaaa tttttgtttt ggtgagatta gaaaactaat
    24961 tacaaataag aatgccgctg ttaaaacaat acttgatcct gctgctatgt taaaactata
    25021 accaataaac aatccaagca atgatgaact tgctccgact gttgcagaaa taaatatcat
    25081 agttttcaag ctatttgcat ataaatatgc tgtagctgct ggtgtaatta acattgcaac
    25141 aattaatata gttcccacac tttgcatagc tgttactgct actaaagtta atagaaccat
    25201 caatagataa tgataaaaac tgacattcat ccctattgct tttgccatta atggatcaaa
    25261 agaagtgatt aataattctt taaaaaatag aactactata accaacacta aaaccgaaac
    25321 gccaattgta atccacttat caatatcttg tacagctaaa atatttccaa ataaaatatg
    25381 aaataaatcc gttgagctat ttgcaactcc tataaggata actcctagtg ctaaaaaaga
    25441 actgaaagta ataccaattg ctgtatctcc ttttattaca ctattgcttt ttataaaagt
    25501 aataatgata gaagctaata gaccaaatac aatcgcacca ataaaaaaat taatacctaa
    25561 aatataagaa attgctactc ctggcaaaac agcatgagaa atagcatcac ccataagaga
    25621 cattcctctt aaaataataa aacaacctac tgttccagaa acaatcccta taactacaga
    25681 tgtaattaga gcattctgta aaaagtgaaa gtcatacaga ccattaataa aattttgaag
    25741 catattattc cccctctcca ataaatatag tatctccata ggcttttttt aaattttctt
    25801 cattaaatac agattctaca cttccgtgag caattagatt tcgattcaaa agaataatat
    25861 tatcaaaata cttctttacc ttgcttaaat catgatgcac aattagcact gtttttccct
    25921 gatgtttcaa tttttttaac gtatccataa ttattcgttc actaactaag tctatcccta
    25981 caaatggttc atctaaaaat atgaaatccg cattttgagc aagacatctt gctaaaagta
    26041 cacgttgaaa ttgtcctcca gataattctc ctatttgatg attagaatat tttcccatat
    26101 cgaccttttc caaagccttt gaaactcttt gccattctgc atttttaatt ctttgaaaaa
    26161 ctttcattcc ggcatatgtt cccatagata cacattcttt tacttttatt ggaaaagtaa
    26221 aatcaatatc agctttttgc tctacatatg caatcttcac cagtttttta ttgatatctt
    26281 cattattaat ggagacctct ccctgatgag ggataatatt taacattgct ttaagcaatg
    26341 tggattttcc agcaccattt ggaccaataa tacctgttaa agcaccttct tgtattttta
    26401 aagaaatctc gttcaatgct aaaaaatcgt tataacaaac attaagcttt ttcacttcta
    26461 acataacttt ctccttctaa atgataatga ttatcacgat catatggtat acaaaaatta
    26521 tattttagtc aaccctaaat tttatagttt agattttttt ctgttgctga agattagaat
    26581 gacaaaacta tttgaaacta cgcgtatagt aagtagcttt caaaatatat aaaaaaggag
    26641 cgttctcaaa tgagaacatg ctcctaaaac tactttattg gcgtatggaa aaattatact
    26701 tgaagtggat agcgggttga ataagttgat aagcatcaaa acatttataa aaattattca
    26761 agactatgtc tgttccatat acaaatatga aaatcagtaa actccattta acttttttcg
    26821 tagtgtgtct ttttatacat gtttatcgtt attaatagtg aataggaaaa aaactatttt
    26881 tgatcattga caattaaata caatttttga ataacgatcc ctgctattcg agctagaaac
    26941 tcaagtacgc catgacctga ttagcgacaa atacttagag atttcttttt gttctgggaa
    27001 aacaaaatgc catgacaata gcagcaaata atgatacttc cgtaaggctc gccgtaaact
    27061 ggggagaggt gaaattccta tgtgcttacc caaagcaatt caaaaatttc ataactacta
    27121 ttttaggaga tgattttata gaatccagag tctacctgaa taaaaaacaa acctgtgcat
    27181 ttctaggaat cgataataat actctagata agtggattcg tatatataat ttcccagtaa
    27241 taagagttgg tggtgtagtt cgctttgacg tagaagatat taaagacttt atgaatacat
    27301 ttaaaataac ttgaaatttt cttctcgccg catatactaa aattagtgaa atatactgcc
    27361 ttactttttt taaggagtga ggattttgcc tacaaagctt tcaaatggga aatataaaac
    27421 taatctgaga tatccaaaaa ggtttaaaga aataacagga attgcatcag aaaagtatca
    27481 aaaaacattt ccaaataggc aattggcgat taaagccgaa aatgatatga agaagaaaat
    27541 agaaaaagtt ttgagagagg agaatgcaaa ctcattagaa ttaaaaggaa aaataacgtt
    27601 taaaaaattt tacgaatcta aatggcttcc tagatatgaa ttaggtcaaa caattagatc
    27661 aaacaggcct cctagtgata taaccattag taatacaaaa gatatatttc ggttgcatat
    27721 tttacctatg tttggtgaat acgcaatgaa ttatctaaat atcaatactg agataatatc
    27781 agatgaatta accaaaaaat cgaaagaata tgcaaatata aaaatcataa aaggatatgt
    27841 tcgttccatg ttcgatattg cagaaatact aaattatatc gaatttaatc gaactactaa
    27901 aattattcaa agtatcactg cacctaaaaa aaatgcttta gaagaaaaaa gaatacaaga
    27961 aggtaagcaa gcactatcat caaaagaact tactaattgg atagaagcag taaatgatga
    28021 ttttaataat catttactta cttttcatga ttacacactt tttatgatta ccctgtatct
    28081 tggtgataga aaaagtgaga cttacgcttt gcaatggaaa tatattgatt ttgaaaaaca
    28141 aacggtccgt cttaaacata ctttagataa atatcaaaga aaaaagttta caaaaggtcg
    28201 aaaagatacc gtcattcaag taccagaagt tgtaatgaca ttgctaagtg aatggaaatc
    28261 ggtacaagct gatcaattgt tgaaattaaa aattaagcaa acgttagatc agtatctctt
    28321 tacttatact aaaccatctg gagaagtaaa ttgtcctgtt catgccgatt acctaaacta
    28381 tcgaatcaat tcaattaaaa gaaggcatcc tgacttagtt catttatctc cacacaaact
    28441 ccgtcatact tatgcaacta tagctagaca aggtggagca gatatgaatc aaatttcaaa
    28501 tgctttaaca cactcagata tttctacaac aaagatatat gtgaacacac cagatatagt
    28561 tgataaggct gtttttgaag cttttcagag aggattgaat aagtgtgatt gatatgtgtt
    28621 cttaaatgtg ttcttaaaaa caaaagaagc actttccaaa taattgaaaa gtgccagaaa
    28681 cgttgatata taaaggatat taacgttttg agaattgtga agctttacgt gctttcttaa
    28741 gacctggttt tttacgttcg accatacgtg cgtcacgagt aagtaagcca gcacgtttaa
    28801 gagctaaacg gaagtcagga tcaacttcta gcaatgcacg agcgatacca tgacggatag
    28861 ctcctgattg tccagcgtat cctccgccgt ttacgttaac aaatacgtca tatgcgcctt
    28921 ttgtttcagt aacagcgaaa ggttgattga ttacttcacg caagtcagca tgtggaatgt
    28981 attcttcaac atctttttta tttacagtaa ttttgccagt tcctggtact agacgtacgc
    29041 gagcaactgc gtttttacga cggcctgtgc cgatatattg aacttgtgcc aatgaaattc
    29101 cctcctatta gattaagttt gtgatgtcta aaacttctgg ttgttgtgct gcatgtggat
    29161 gttctgctcc accgtatacg ttcaattttg taaattgttt gcggcctaaa gtgtttttag
    29221 gaagcatacc ttttacagaa gtttcgatca aacgacgaga atttttagca cgcaattcgc
    29281 cagcagtgat tgatttcaat cctcctgggt agttgctgtg acggtaataa attttgtcag
    29341 tcgctttttt acctgttaat ttcactttgt ctgcattgat gacgatgacg aaatcgccag
    29401 tatccaaatg aggtgtgaaa gttggtttat ttttaccacg tagtacagat gcaacaactg
    29461 ttgaaagacg tcccaatgga acatcagttg cgtctactac ataccattta cgttctactt
    29521 cgcctggttt ggccatatat gttgtacgca cgttattttc ctccaattta agttctgatg
    29581 tttgacatac acttcgagtt tccggggctc tttgtggggc aaacaatacc atttaacata
    29641 ttacccgcta atctagtcaa tgtcaacgtt tttctgcttg attgaaaaat ttatatgaaa
    29701 ctttacttct tttctttctt ttatataaaa gggtcgtttc ctgtcaaaat gaaaaacaaa
    29761 aagaggctgt gacaaaagtc ttgtcacaac cccttattt
//
LOCUS       DBNICI010000016        29098 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.016, whole genome shotgun sequence.
ACCESSION   DBNICI010000016 DBNICI010000000
VERSION     DBNICI010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 29098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.016"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..1030)
                     /locus_tag="AC1ZCC_000587"
     CDS             complement(<1..1030)
                     /locus_tag="AC1ZCC_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIQ1628862.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="AC1ZCC_000588"
     CDS             complement(1112..1801)
                     /locus_tag="AC1ZCC_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIQ1628863.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="AC1ZCC_000589"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="AC1ZCC_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIQ1628864.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /gene="rplJ"
                     /locus_tag="AC1ZCC_000590"
     CDS             complement(2328..2831)
                     /gene="rplJ"
                     /locus_tag="AC1ZCC_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIQ1628865.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3035..3162)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="AC1ZCC_000591"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="AC1ZCC_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIQ1628866.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="AC1ZCC_000592"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="AC1ZCC_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIQ1628867.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="AC1ZCC_000593"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="AC1ZCC_000593"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HIQ1628868.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="AC1ZCC_000594"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="AC1ZCC_000594"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HIQ1628869.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7543)
                     /locus_tag="AC1ZCC_000595"
                     /pseudo
     CDS             complement(6443..7543)
                     /locus_tag="AC1ZCC_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7635..8522)
                     /locus_tag="AC1ZCC_000596"
     CDS             complement(7635..8522)
                     /locus_tag="AC1ZCC_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIQ1628870.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="AC1ZCC_000597"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="AC1ZCC_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIQ1628871.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="AC1ZCC_000598"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="AC1ZCC_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HIQ1628872.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="AC1ZCC_000599"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="AC1ZCC_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIQ1628873.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /gene="cbpA"
                     /locus_tag="AC1ZCC_000600"
     CDS             9753..10397
                     /gene="cbpA"
                     /locus_tag="AC1ZCC_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HIQ1628874.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="AC1ZCC_000601"
     CDS             complement(10453..10956)
                     /locus_tag="AC1ZCC_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HIQ1628875.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="AC1ZCC_000602"
     CDS             11553..12518
                     /locus_tag="AC1ZCC_000602"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIQ1628876.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="AC1ZCC_000603"
     CDS             complement(12617..13789)
                     /locus_tag="AC1ZCC_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1628877.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="AC1ZCC_000604"
     CDS             complement(13776..14921)
                     /locus_tag="AC1ZCC_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1628878.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="AC1ZCC_000605"
     CDS             complement(14935..15867)
                     /locus_tag="AC1ZCC_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1628879.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15954..17063
                     /locus_tag="AC1ZCC_000606"
     CDS             15954..17063
                     /locus_tag="AC1ZCC_000606"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIQ1628880.1"
                     /translation="MTFVTMKESAMHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYL
                     LIAVLFTGVSYLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFL
                     LGFLLISTVGNHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQEL
                     SEQNKALALSQETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLK
                     EPLENLSATVHTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNE
                     TQQNCFLMVIKEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGL
                     KTMRQRVQEQKGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17678
                     /locus_tag="AC1ZCC_000607"
                     /pseudo
     CDS             17060..17678
                     /locus_tag="AC1ZCC_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(17788..18015)
                     /locus_tag="AC1ZCC_000608"
     CDS             complement(17788..18015)
                     /locus_tag="AC1ZCC_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIQ1628881.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18118..20706)
                     /gene="recD2"
                     /locus_tag="AC1ZCC_000609"
     CDS             complement(18118..20706)
                     /gene="recD2"
                     /locus_tag="AC1ZCC_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SF1B family DNA helicase RecD2"
                     /protein_id="HIQ1628882.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20726..21385)
                     /locus_tag="AC1ZCC_000610"
     CDS             complement(20726..21385)
                     /locus_tag="AC1ZCC_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIQ1628883.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21434..21937)
                     /gene="trmL"
                     /locus_tag="AC1ZCC_000611"
     CDS             complement(21434..21937)
                     /gene="trmL"
                     /locus_tag="AC1ZCC_000611"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HIQ1628884.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22158..23024)
                     /locus_tag="AC1ZCC_000612"
     CDS             complement(22158..23024)
                     /locus_tag="AC1ZCC_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIQ1628885.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23133..23774
                     /locus_tag="AC1ZCC_000613"
     CDS             23133..23774
                     /locus_tag="AC1ZCC_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HIQ1628886.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23836..25197)
                     /gene="mgtE"
                     /locus_tag="AC1ZCC_000614"
     CDS             complement(23836..25197)
                     /gene="mgtE"
                     /locus_tag="AC1ZCC_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIQ1628887.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25215..26114)
                     /locus_tag="AC1ZCC_000615"
     CDS             complement(25215..26114)
                     /locus_tag="AC1ZCC_000615"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIQ1628888.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26116..26913)
                     /locus_tag="AC1ZCC_000616"
     CDS             complement(26116..26913)
                     /locus_tag="AC1ZCC_000616"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HIQ1628889.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26891..27595)
                     /locus_tag="AC1ZCC_000617"
     CDS             complement(26891..27595)
                     /locus_tag="AC1ZCC_000617"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HIQ1628890.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27726..28301
                     /locus_tag="AC1ZCC_000618"
     CDS             27726..28301
                     /locus_tag="AC1ZCC_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HIQ1628891.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28419..29096
                     /locus_tag="AC1ZCC_000619"
     CDS             28419..29096
                     /locus_tag="AC1ZCC_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HIQ1628892.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8562 a         6167 c         5231 g         9138 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgcgaa acccatcgtc ccgatgtttt gctgacggac atccgcatgc
    17221 ctaaacaaac gggcatcgat gctgccaaag aaattttggc taaatttccc aaagccatcc
    17281 ttttgctttt gacgacattc aaagatgaag aatacatccg cgaagcattt tccatcggtg
    17341 tcaaagggta tttaatcaaa cagaacttgc aagcgattat tccttctgtc aaatccgctt
    17401 ataatggaca ggccgtattt ggtaatgaaa tcatcgagac tttcactcaa atgatgaaga
    17461 ataaaccgac gattgatagt catgcttatg cttcttttag tgaacgtgag cttgcaatca
    17521 tcaaagaagt agctgccggg aaaaacaata aagaaattgc agatgccttg tacttgagtg
    17581 acggcactgt tcgcaattac atcagccaat tactagaaaa gctagattta cgggatcgca
    17641 cgcaattggc tatctatttt tatcagcatt tgcaataagt cttatctaac acaaaaaaac
    17701 ctgaatgatg ctccttcgca tcattcaggt ttttttcaca tcttctattc aattgtcccg
    17761 caaaccattt cctcacgatt gaaagaatca aggattcgca gggctgccag gattcgttgg
    17821 attgttcggc gtgttaggcg gaaaaatagg cgccttgtct aacgtttttt ctagttttga
    17881 ttcttcacga tcgtctcgtt ctacatcagg actttcattc aatttgaatt ctttttccat
    17941 ctcatctaac tttttaaagt cattgccaga ttcttttgct aatttcgcta atttatcttc
    18001 ggtttcttta gccataaagt aacgcccccc ttttgatttc agcttagcat atcacactga
    18061 aaaaacaaaa gaaaacgcta accaacgatc atttttagct atttctcatc tttaaaatca
    18121 agcatttttt gccggtataa aatcgtaggg agaactgttt tccataccaa tcatcggatc
    18181 tactaactgg ttatcgatca cttctgctgt aagaacgaaa tctttagaaa cagcaggttc
    18241 tacctcttct ttcttttctt ctccaacaaa tgaagaaaac agatctggtt ccactgtttc
    18301 cattacttgg tttttcgttt cattcgtccc actgtcatct tctacaggta gcttttcttt
    18361 tacaacccgt ggctcttttg tatagttccc atcttcaaat ggatcatttc cgaccaaaga
    18421 atcttcataa gctgctagtt tggtccggag agtgatattc attttatcat tatccatgat
    18481 ccttgatttc aacgtagtca tccggcttgc tgattcattc gctacacaag tgacataggc
    18541 ttgctcttcc cctaacaaaa tcaacagttc cttactgcga gtcactgccg tgtagagcaa
    18601 attacgctga agcatccgct gatattgatg gaccatgggc aaaataacca tcttgaattc
    18661 actgccttgt gatttatgga ttgagcaaca ataagaaaga gtgattttat tccattcgtt
    18721 tcttttataa gtgacctcat ttgcatcaaa ctggataact aattcatcca ctttatcttc
    18781 agaatctttt gccaagatga tacccgtgat ttctcccata tctccattga atacattcaa
    18841 ttcgggagta ttgactaatt ggaggacttt gtctcctatc cgataaacgg tgtcgttcca
    18901 atgtacttcc ttttttccag gtttccctgg attgaatatc tcctgcatca ttttatttaa
    18961 cgcatcgatc cctgcaggtc cccgatacat tggtgctaat acttggatat cttgtgcacg
    19021 gaatccctta tttttcgctt tttccacaat tttagagatc aagggttcaa tttgataagc
    19081 atcacttcgg aaataggaac gatctttttg attctttgtg aaatcttttg gcagacgtcc
    19141 ttctttgatc tcatgggcaa gaggaataat actagaacca tcaccttgcc gataaatctg
    19201 attgagttcc attttgggaa tttgttcgat ttccaaaaga tcatggagta cttgaccagg
    19261 accgacagag ggaagctgat ctttgtctcc tactaagata acttgcatat tcgtcggaat
    19321 cgctttgaat aatgtgttag ccagccatgt atcgaccata gacatctcat caacaatcaa
    19381 aagtccgcct tctaattctt tcgtactgac tgaaggtgtt ttttcccgtc cattcagccc
    19441 cagaagacga tggatcgtac tggcaggcag acctgtcgtt tcgttcattc gtttagctgc
    19501 tcggccagtt ggtgctgcaa gtagaatagg aaaaatttct tgggtatatt ttgaaggatc
    19561 caattccagt ccgttcaact ctgcaaaaag attgacgatt ccgttgatca cagtagtttt
    19621 ccccgtacct ggtcctcctg ttaaaataaa tagaggggaa cggattgctt cagcgatagc
    19681 ttgttcttgc gaaggtccat actgtatgcc gaatattttt tcgatacgcc gaatattctt
    19741 ctgcacttct ttttcaggat aatggatttc ttttttacga ctgagaaggc gctggatcga
    19801 attaccgatc ccccattcag aaaagtacaa actgttttcg taaagattag tctcttcttg
    19861 ttgtatcttt ccttcttcaa caagttggat aattacagta gcaacctgat caggctggat
    19921 ttcaattggg cgacttgttt ctaaaatacg gatcgtttgc tgaagcaatg tctccgccgg
    19981 tacgtatgta tctcctgttt gaactgcatg ctgaaaaatc tgatgcaaga tagctgctcg
    20041 aattcttctc ggtgaatctg ctgcaatacc gatttgttcg gctagattat ctgcacgttt
    20101 gaacccgatt ccttctatgt cttcgactag ctggtacgga ttttcttcga tgatatccaa
    20161 agactcgttc ttataggcct ggtagatact gaaagctaac tgactaccga acccgtaacg
    20221 attcagtccc atgatcactt gatccatccc gtggtttagc cgaatcgtct cagcaagcat
    20281 ttctcttttt ggcttcgtca aaccggagat ttgttttaat accgaaggat cagcaatgat
    20341 tttttcgata gcatcttctc ccagcaattc tacaattttt tctgctgttt tcttcccgat
    20401 tcctgggaat ttttcgctgg aaagatacgc tacgatccca tttgcagttg ttggcttttc
    20461 ttgctcatat ctttcgagtt taagctgcct tccgtattta ggatgatcca ctaattctcc
    20521 ataaaaacga taagtctcat tttcttgaat atcgccgaaa cttccggtga ctacaatctc
    20581 tttttcacgg taatcagctg tggtttcact gatgtgtacc aaaagcactt tgtaaaaatt
    20641 actaggattt tgaaaaaaaa tcgcggcaac tgtaccaacg aaataaggca tttcttctgg
    20701 ctgcatcgga agccgcctcc ttcttctata gtaaaggttc tggtgctgtt tcagttccta
    20761 aaaaatctgt ttggttccat actttcagtt catatggcaa taatctgcaa ggctcttcag
    20821 tctctaaaat cgtcaaacta ctattgaaca atccacccat ttcacgcaac tcacttagct
    20881 cttttcctgt catccattga atggctgcag ttaaagaagc tccatgtcct acgaataaaa
    20941 gtgggccatc tttatgtcgg gcaaccgcat ctgctacaac agtttcgata cgtgtgattg
    21001 ccgattcaat cgtttctccg tgaaaaggag aaggatcata cagatctaaa tgattacgca
    21061 aattcggcag attatccgga tatttctggt acatttcatc gatcgactga ccttctaaag
    21121 tacccagccc tagttctctt agcttatctg tataaaaaat ctctaccgga cgagcaagct
    21181 ctttgttgat accttctgct gtatcacgtg ctcgctttgc tgtactggaa taaattgctt
    21241 taaaaggaac atattttacc tttttcccaa atgctttgat ttcttcgtaa ctttgtggca
    21301 aaagcggaga atcgccattg ctcccttgaa aacgtctttc ctgattccat tgtgtcttcc
    21361 catgtcgggt aaaatatagt tccatacgca gcaaactcct gtctttcttt tattttactc
    21421 tataataaac tgtctaatcc agcttgtcat tttcatagtg atgcgtcaat tctaaattag
    21481 ggaatccttg ctggcgaagt gcttcataca ctatcattgc agctgtattc gataaattca
    21541 atgagcggac atgctcatca ttcataggga tgcgtaaaca cttttcttcg ttttcacgca
    21601 taaatgtttc tggcaaaccg gttgtttctt ttccaaataa aaagaagtgc tcttctccat
    21661 cagcaaaatt ttcgtcacta tatactttat gggcaaattt tgagatcaaa tgtagtttac
    21721 gattacctaa aaatgttaaa aaagcttcta aattactgtg gtaagtaata tttacatcat
    21781 tccaataatc caaccctgcc cgttttagat gtttgtcatc agttgaaaaa cctagtggct
    21841 cgattaaatg aagcggagta ttcgtggctg cacacgtacg ggcaatattt ccagtgtttg
    21901 cagggattaa tggttcaaat aaaacgatat gattcatcag gatcattcct ctcatattta
    21961 tgttcgtttc tattttctga caatttgatt tttgcataaa aaaaaacgta tcaactcggt
    22021 gtcagtcact gcgagtcgtt acgttagtga agctcttctc actaactttt atcagaacag
    22081 gaacctgata aaaaccaatg aaaaaacaac ttgttgctaa tataacaccc tcaacttttt
    22141 tttgcaacct ctactcatta aaaactgcta gaaaaacgtt ttctcttagt tccttttaat
    22201 aattctatgt caattgtaat cttcttcaaa ggattcttat ataagtctgc tttgattcgc
    22261 tcttctacct gccattctaa aggtgacatg gccaaaagat ctttttgatt ttcctctggt
    22321 atctcaaatt cataggttac acgctgacgt tcagttaatt tcattgcttc agcaaacttg
    22381 gctactacac gttcattgga ataactctgt ttttcaggtt gattcggata aaaagcctga
    22441 cgtagttcct ttaaataatt ttcttgtggc accaccttca acacttctcc atcttctgag
    22501 agtacacgtt ggaattcttg gtaatgagaa ggcgaaaaga tatttaaaac cgcgttgaat
    22561 gtttgatcag caaacggcaa attggtcaaa tcagctacac accaaaatgc ttcggcagat
    22621 tgttctgtag ctgcgtagac tccttctttt gaaatatcaa atccaacagc attttgaatc
    22681 tttttcaatt ttgaaagttc attcaagaag ctaccttctc cgcagccaac atctaatagc
    22741 gagacttctt caggtaaatg ctgcgcaata gtctcaataa cgggctgata catgccagat
    22801 tggatcattc tacgccttgg aacgaacatt ccttgatcgt attctgtttt aaccgcatgg
    22861 gataaaaaat aaagtgttcc ttttttggaa aggtcaaaac ggtgattatt tttgcatatc
    22921 aagccattat cagacttttt tacttcttct tggcaaaccg gacagcggaa cagctgatga
    22981 ttttctgcca aaaactggcg tcctcggtca atttttttta acatagttcc ccctcatttc
    23041 tgttttatct taccgttaga tgctcacttt ggcaactgct aaaaatatga tacaatgcct
    23101 agaagaaagt gaaagataaa ggagaagatt agatgatcaa agaattttgc gctgaaaact
    23161 acacatctat tcctctagca attgctagag gagccgatcg gatcgaattg tgtgacaact
    23221 tagcggtcgg tggcacaacc ccgagtactg gtgtgatcga agaaacgtta agttatgctg
    23281 gggaaaaaga tatccccgta atgacgatta ttcgtcctag agggggcgac ttcgtctata
    23341 acgatatcga attgaaaatc atgcatactg atttgattga agcaaagaaa ttagggacgg
    23401 atggcgtagt atttggttgt cttactcctt caggctggct agacgaagag gccttagaac
    23461 tattgatcga aacagcagaa gggttgcaga tcaccttcca tatggctttt gacagtattc
    23521 ctgaagatcg gcaattcgaa gcaatcgatt ggctggtaga acatggtgtt gaccgtatct
    23581 tgactcatgg cggtcctgct gacaaaacta ttgtagaaaa tttcccacgg cttaaagaat
    23641 tgattgccta tacagatggc cgcttaatca ttttgcctgg tggtggtgtc actgcagaga
    23701 atgcagcaca tgtagctaaa gaattgaacg taagcgaagt acacggaacc aaaatcgtgg
    23761 atcttcagtt ataatcttat aaacaaaaaa acatttgcaa caggcataat gcttattgca
    23821 aatgtttttg tttttttaca taaaatagcc cataaataaa gaagcaatat tgaaataaat
    23881 cagtacactc gtcaaatcgc tcaaagtcgt gataaaggga ccgcttgcta ctgctggatc
    23941 aaatcctaga cgatccatta gcatcgggat taaactacct gcaagattcg ccactgtgat
    24001 tgcaaatagc atagccatcc ctatgacaaa ccccaagata aaattatgtt tccaaaaacc
    24061 tacgattaga aaaatcgtga tccccgtaac agctcctgtg acaacgccgg tcaagacttc
    24121 agccaaaaca agtcgaacaa atccatgatc tttctcatca gaaatcgcaa gtcgacgaac
    24181 agccacagct agcgactggg ttccagcatt accggcagtt cctgtaatca gcgaaatgaa
    24241 gactgctaaa atactcgctt cacttaccaa gtcttcatag tgactgatta aagaagcggt
    24301 cgacatacct aaaaacaaca gagtgatcag ccaaggcaaa cgtcgagacg ctgcctttac
    24361 tggattttca ttaatttctt ccacattgac cccggcaagt cctgaataat cgctagcagc
    24421 ttcatcatca ataacgtcga tgatatcatc gactgtgacg attcctaaca aatgatcatc
    24481 ataatcggta acaggaagag ctaagaagtc ataatctcta aatgtttgtg ccacatcttc
    24541 ttggtcgtcg cctacatgaa ctgaaagcac tcgttcgctc atcagatcag aaatcattgc
    24601 gtcgtcatca ttcacgatta ggtcgcgcag agaaataacc cctactagct gaccttcttg
    24661 attaacgaca tagatgtaat agattgtttc agctacatct gcttcgttct tcagcacgta
    24721 catcgcagag cgaacagttt gattggctac gatcgaaaca aattcagttg tcatgatcgc
    24781 gccagccgtt tcatcctcgt aatggagaag ttccttgatt tcactggcat cgtctgccga
    24841 catcaaactt aaatatttag caatttgctt tttatccagc gtattaagca agtcaacagc
    24901 attatctgta tacatctcag aaagcatatc agctgcatag ctaggacgca tttcatctag
    24961 gtattctttc atatgttcgt tgtcttcttc aatcacatcg aacatatccg ctaattcttt
    25021 aggcgacaga taggaataga tcagctgcct gttctcttct gtaagagatt ggtagaattg
    25081 cccctgctca tagatatgca tctctaaaaa actgtttctg aatgcctgaa tattattttc
    25141 ccttaaatct tgagtcaact gggcaaatct ttcctctaat tcttgatttt catccaaacc
    25201 aatctcacct cgccttattc ttcaaaccgt ctcacaactg cctccatgtc tgcggctaaa
    25261 ggctgcttga actccagcaa ctcttctgta aatggatggt aaaaacgcag atcataacaa
    25321 tgaagggctt gtctttttat tcccagatcc attcgcccgc catacatatc atcaccgagc
    25381 agggcacagc caattgcgga aaaatgaact cttatttgat gtgtccttcc tgtatgcagc
    25441 tggatatcaa ctaacgcgat ttctgttagt cgcttctcta tccagtactc agtctgtgcc
    25501 gaacgtcctt catcagagac cattcgcttc aatagcgaag aataatctcg ggcgatcggc
    25561 aaatcgatat gtccatgtgc cgataaatct gaaagccttc cactgactaa ggcctgatat
    25621 tttttcacaa acttcttttc acgaagttgc atatctaatt ttgcgtgcgc aaatccatgc
    25681 tttgcaaaaa gcatcaagcc agatgtatcc cgatctagtc gcgtcaccac atggattact
    25741 tggttttcgt aaccttgttt tttataataa gcctttacac gattagccat tgtcccattt
    25801 ggatggtatt gggcaggaat agacgaaacg cctgctggtt tatttactac cagaacgtgt
    25861 tcatcttcat acacgatatc gatcggtgtt tcatccaaaa gtacggtttc atgttctcct
    25921 tcagcaggaa tcacaatggt taccttatca tccttagcta gagaaaacaa gacattttct
    25981 acttgatcat taactttgat ttgaccgcct tgaaatttta ttttcgctaa cagacccttt
    26041 gagatccctt tttcttttaa aaaatatttt acttgctgag aatgatcttt ctcataatcc
    26101 catgtaaact gcatatcaat cctcactaat aaaggcatct ttcacacgat gccaaaaatg
    26161 catatgtcga taagaagcaa aatggatccg ttcttctgct attcgataat aaattgagcg
    26221 gatatttgct tttgcgacat tcaattgatc aaccgttaca agataatctt ctgtattttc
    26281 caattttatt tctagccatt catcatgggc aatcacgatc ggagaaccca aagtacgaaa
    26341 cactcgatta ttcagtgaag caatctctgc caactgaaag gcattgatac taggatggat
    26401 cacagcaccg ccgacacttt tattatatgc tgtcgaacca gtaggcgtag aaatcgtcaa
    26461 tccatctcct ctaaaacttt caaacagctc gtttttgatg tatacatcgg ctaccatcgt
    26521 acgattggcc cgtttaatcg ttgattcatt cagtgctaaa aaatgctggt ccggtgtttc
    26581 atccaaataa ctgatccgaa catccagcaa gggataacta acactttgtt cccggttcgt
    26641 tcgtaagctg tctactagtt cttccagctc gtaatcccgc caatccgtgt aaaaccctaa
    26701 atgacctgta tgcacgccca agaaacgcac atcattcaga caatgactgt agtgatggaa
    26761 cgccgacaat aatgtgccgt ctccgccgac tgagatgacc aattctggct catgatcatc
    26821 tcgttgaatc cctgcttgtt ctaaaagtgc gttcaaacgt tctgtcactt ctatagtctt
    26881 tgcttcttta ttatgaacga tcgctacttt catcccctgc ctcctctctt tcgctttctt
    26941 tcttcaaaag cgttcgatag tagacatcat ctgattttcc tcttccatgg gagaataacc
    27001 gctgtgcttc ttggatttct tcacggatac tggacatctc ttcatccaac tgataggcag
    27061 cttctgccgc acgctgaagt cgttcactca tttcttctgg aaaaacgcct tgatatttat
    27121 aattcagcga atgctcaatc gttgcccaga aattcatcgc aagagtccga atctggattt
    27181 ccgccaaaat tttcttctca ccgctgatca gctgtacagg atactcgatc accagatgat
    27241 aagaacggta gccacttgct tttttatttg tgatgtaatc tcgttcttgg agaactttca
    27301 tgtcttttct tttacggatg atctcaacaa cttgatggat atcttctaca aactgacaca
    27361 tgatccttaa cccggcaata tcagacattt cttcttctaa acgtcccatt ggaatatgcc
    27421 gtaattgtga ttttgttaca atactatcga ctggcttgac tctcccagtg acaaattcaa
    27481 tcgggacatg tcgattttgt tcacggaact gtttgcggat cccccgcagt ttcactttca
    27541 attctgatac agtttgttca tatggtgcta aaaatgcttc ccaatctcgt tccatagctg
    27601 atacctctac ttcattaaca ttcattgttt attctaccat atatgcaaag ttttttcgta
    27661 ctcttgattt atgcattgac caaaaaaaga aaattaggca aaatagacaa aaaggagtcg
    27721 atcaaatgac acaaatgctt gaaatcgaat ataaatctat gctgacaaag caagagtacg
    27781 agatattcat cgctcactat caattaacag ataaagattt ccgtgtacag actaatattt
    27841 atttcgatac cgttgatgaa cagctgaaga aacaaaattg tggcctacgg atccgtttcg
    27901 ttggtcatca agctgaatat acattaaaaa cacctgctga aaaagggcgt ctcgaaacaa
    27961 cggatacctt tataacagaa gaagcagaag catttatctt cgaaaaacgt ctgcctcgtt
    28021 caggcgcagt ttttcaaaaa ctttctgatt tgaatatcga tcctgcttca ttaatccaaa
    28081 ccggaaagct tacaacaaaa cgagctgaat tccctattga agaaggactt ttagctattg
    28141 atgaaagctg gggagacaat ctacacgatt ttgaactgga actagaggta ggggatgcat
    28201 ctgtgggaaa aattgctttt ataaattttt taaaacgttt ttctttacct tatcgtcctg
    28261 ctaagaacaa gatccaacgt atgcttgaag ctaaaaatta gaaacctttc attttcacaa
    28321 ggcacttgag ttcatttttt tacttttttc tgttaagtta taggatgtga aagaatctag
    28381 tcactagttg tatagaatct attttggggg agattcatat gatcgaaatc tatttatttg
    28441 ttaatccttt aggggaatac tgtttcgaaa tggaacaaca gttattacaa tttatcgaag
    28501 aagagtacgg gacaccatca aaagaaaaga tgcaatttcg ctttcttcct ttggtcaatt
    28561 tgcagacaat tggggatgtc atgcaacaaa aaggaatttc tcagaacaat cttgaggaac
    28621 gtaatcactt attctcaatg acttattctg cagcattgga ctgcaaagcg gcgcagtttc
    28681 aaggaaagaa aaaaggccgc cgttttttga tcaaattgca agaagcagtc ggctgtaatg
    28741 acataccata ttctcaagaa cttgctgaat ccatatttga agaaatcggc ggcgatttga
    28801 atatgtttaa agaagatcgt cagtctgact tcgttaaaga agtattcttt tctgatcaaa
    28861 aaattgctcg ggaaatgggc atcacaaaac acccttctgc agtcgtttac aattatgcct
    28921 gcgaccgtga ttttggtgtt ttagtagaag taaaaggtac aagtagcctt tggagaatca
    28981 cagagctgtg taaaacaaca gataactcat atcaagtttt taatggtgaa catttacata
    29041 caaatgattc tcagttccat tcaagaacta gccatttaca cttgctttct aattaaaa
//
LOCUS       DBNICI010000017        27948 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.017, whole genome shotgun sequence.
ACCESSION   DBNICI010000017 DBNICI010000000
VERSION     DBNICI010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.017"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(5..586)
                     /locus_tag="AC1ZCC_000620"
     CDS             complement(5..586)
                     /locus_tag="AC1ZCC_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIQ1628893.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(769..1395)
                     /locus_tag="AC1ZCC_000621"
     CDS             complement(769..1395)
                     /locus_tag="AC1ZCC_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HIQ1628894.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1417..2748)
                     /locus_tag="AC1ZCC_000622"
     CDS             complement(1417..2748)
                     /locus_tag="AC1ZCC_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HIQ1628895.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2903..3304)
                     /locus_tag="AC1ZCC_000623"
     CDS             complement(2903..3304)
                     /locus_tag="AC1ZCC_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HIQ1628896.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3390..3596)
                     /locus_tag="AC1ZCC_000624"
     CDS             complement(3390..3596)
                     /locus_tag="AC1ZCC_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628897.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3691..5193)
                     /locus_tag="AC1ZCC_000625"
     CDS             complement(3691..5193)
                     /locus_tag="AC1ZCC_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HIQ1628898.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5211..5666)
                     /gene="argR"
                     /locus_tag="AC1ZCC_000626"
     CDS             complement(5211..5666)
                     /gene="argR"
                     /locus_tag="AC1ZCC_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HIQ1628899.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5764..6381)
                     /locus_tag="AC1ZCC_000627"
     CDS             complement(5764..6381)
                     /locus_tag="AC1ZCC_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HIQ1628900.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6602..7975
                     /locus_tag="AC1ZCC_000628"
     CDS             6602..7975
                     /locus_tag="AC1ZCC_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIQ1628901.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8050..9162
                     /locus_tag="AC1ZCC_000629"
     CDS             8050..9162
                     /locus_tag="AC1ZCC_000629"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIQ1628902.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9234..10871)
                     /locus_tag="AC1ZCC_000630"
     CDS             complement(9234..10871)
                     /locus_tag="AC1ZCC_000630"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HIQ1628903.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11039..11647)
                     /locus_tag="AC1ZCC_000631"
     CDS             complement(11039..11647)
                     /locus_tag="AC1ZCC_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIQ1628904.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(12031..13182)
                     /gene="queG"
                     /locus_tag="AC1ZCC_000632"
     CDS             complement(12031..13182)
                     /gene="queG"
                     /locus_tag="AC1ZCC_000632"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HIQ1628905.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(13201..13953)
                     /locus_tag="AC1ZCC_000633"
     CDS             complement(13201..13953)
                     /locus_tag="AC1ZCC_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628906.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(14094..15044)
                     /locus_tag="AC1ZCC_000634"
     CDS             complement(14094..15044)
                     /locus_tag="AC1ZCC_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HIQ1628907.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(15045..15884)
                     /locus_tag="AC1ZCC_000635"
     CDS             complement(15045..15884)
                     /locus_tag="AC1ZCC_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HIQ1628908.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(15881..16627)
                     /locus_tag="AC1ZCC_000636"
     CDS             complement(15881..16627)
                     /locus_tag="AC1ZCC_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HIQ1628909.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            16865..18235
                     /locus_tag="AC1ZCC_000637"
     CDS             16865..18235
                     /locus_tag="AC1ZCC_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIQ1628910.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(18290..19186)
                     /locus_tag="AC1ZCC_000638"
     CDS             complement(18290..19186)
                     /locus_tag="AC1ZCC_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIQ1628911.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(19300..19470)
                     /locus_tag="AC1ZCC_000639"
     CDS             complement(19300..19470)
                     /locus_tag="AC1ZCC_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628912.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(19467..20144)
                     /locus_tag="AC1ZCC_000640"
     CDS             complement(19467..20144)
                     /locus_tag="AC1ZCC_000640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HIQ1628913.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(20235..21140)
                     /locus_tag="AC1ZCC_000641"
     CDS             complement(20235..21140)
                     /locus_tag="AC1ZCC_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HIQ1628914.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            21315..22769
                     /locus_tag="AC1ZCC_000642"
     CDS             21315..22769
                     /locus_tag="AC1ZCC_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HIQ1628915.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            22785..23816
                     /locus_tag="AC1ZCC_000643"
     CDS             22785..23816
                     /locus_tag="AC1ZCC_000643"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIQ1628916.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            23823..25010
                     /locus_tag="AC1ZCC_000644"
     CDS             23823..25010
                     /locus_tag="AC1ZCC_000644"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HIQ1628917.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            25166..26548
                     /locus_tag="AC1ZCC_000645"
     CDS             25166..26548
                     /locus_tag="AC1ZCC_000645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HIQ1628918.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            26585..26905
                     /locus_tag="AC1ZCC_000646"
     CDS             26585..26905
                     /locus_tag="AC1ZCC_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628919.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(27105..27773)
                     /locus_tag="AC1ZCC_000647"
     CDS             complement(27105..27773)
                     /locus_tag="AC1ZCC_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HIQ1628920.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         7778 a         5720 c         5137 g         9313 t
ORIGIN      
        1 attattaaag aaccttttgc gaagttaaca atctttcttt gatcaattga aaattctgcc
       61 taagtagtga tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa
      121 tcaacgcttt ggcttgacgg tctgcttctt catcggacag tttttcttca gaaagtgcca
      181 ggatattttg tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt
      241 attcttcatc cactgtactc aactcaataa tcaaattacc tacaggacaa ccatgataag
      301 tgatctgtgt ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg
      361 gctgttttcc agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg
      421 catggatcac cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt
      481 ttcctgtatc agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat
      541 aaataagatc tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt
      601 ttcatttctc atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt
      661 aaaaaagact gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc
      721 tagatgttca ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg
      781 ctctaatact tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc
      841 tgcatccatt gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa
      901 tccactctct accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc
      961 aagaattact gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg
     1021 tccaaagatt ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc
     1081 ttcgataaaa tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa
     1141 catctgctgc tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg
     1201 tgggtaaaaa taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac
     1261 catttcaatc atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa
     1321 gccaccgcct gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt
     1381 tttattattg aacatggtgg attctccatt cgttatttat tttaatacat catttagagc
     1441 ttcactcatt gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc
     1501 attcatagct agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc
     1561 ccctagaatc aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc
     1621 taatacttgt gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc
     1681 ttgtgctttg gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac
     1741 actgtaaggc acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc
     1801 cataatgatt cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc
     1861 tccaatcgcc caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc
     1921 gcgatctgtt agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct
     1981 tccagttgca gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa
     2041 ggtgacggca gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa
     2101 ttcgattccc gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg
     2161 tggaagaaat tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat
     2221 tgatgcaaat tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg
     2281 ttcctgatcc atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat
     2341 tggtaaaatg attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac
     2401 tttttcacca ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc
     2461 catcactgtc ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc
     2521 cgtcaatttt tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa
     2581 aatcaatgat ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt
     2641 tgacttttca acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt
     2701 accgcccttt ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct
     2761 tcttttttac ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa
     2821 tcatctgctt tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc
     2881 tggttcgatt tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag
     2941 ttgctttgga gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt
     3001 cgctgatgcc atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta
     3061 aattataaag agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga
     3121 tcatatgttg taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg
     3181 gatttgttcc ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg
     3241 gtgcctcgac ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt
     3301 tcattgtact gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac
     3361 aagtaaggcg ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact
     3421 atttttccaa aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa
     3481 taaatagcaa tcttttctac ataatctttt atatgattga ttttaagata catatatacc
     3541 agaaagttga ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt
     3601 tcctcctttt gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt
     3661 ccaagcaact agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga
     3721 aaaatgtccg aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc
     3781 attttctatc tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc
     3841 gtttccgtat ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt
     3901 catccctttt tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc
     3961 gcgtggttca tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa
     4021 ctctttgatt tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag
     4081 gatctttgcg atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg
     4141 tccgcttgtt tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt
     4201 attgattaca tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat
     4261 gaatctttgt agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc
     4321 ttttaatacg attgtactta atactgcgat caaattattt cgattgatca ctgttccaga
     4381 agcactgaca actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc
     4441 atttgctaaa acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat
     4501 atgattagga aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa
     4561 gctattcaat actttttcag caaagaatcc accagcacca tttccggcat caacaatgat
     4621 tttccaacca tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac
     4681 taaatcttgc gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct
     4741 ttttattttt tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc
     4801 tgaggtaaag aattttattc cattataata atagggaaga tggctagcag tcagcataac
     4861 cgcagcatcg cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc
     4921 taattcgaaa tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt
     4981 cagtgctggt ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg
     5041 atcggttgat ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac
     5101 catgtctgtc gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc
     5161 cgatccattt tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc
     5221 attttttcca gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg
     5281 aatgtatcta caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa
     5341 ttcgttacca catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac
     5401 tggatacgca aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca
     5461 gatggctggt taaataatgt atagcgtact attttgtttt cattttgact ttttactaac
     5521 ttcatttctc gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc
     5581 agccgcgcaa gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc
     5641 agtgcttgtc gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag
     5701 ataagtaata tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg
     5761 ttgctaacct ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt
     5821 tcttggatcg ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt
     5881 tgcgatgaat gcttctaatt tctcaaacgt tgctggttct tcggcatagg gcccgacgtg
     5941 taagatttgg gcacaaagac cttcttccat ctttttgaat actacttgtt ctttcaagtc
     6001 ttccggaagt ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc
     6061 aggctgtcgg atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa
     6121 atacttttct tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc
     6181 aggaatcagc caattttttt taggcgacat gcgtattgca tagctgactg catatagaca
     6241 ggctactctt cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa
     6301 tgatttttta ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt
     6361 acgccattca attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat
     6421 catacctctt tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag
     6481 aataagtgaa gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg
     6541 tttctatttt tataatagaa atcactctct ttttttacga aattttttag aggtgatgta
     6601 aatgacaaaa caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc
     6661 ttttgcgggc gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca
     6721 gtttgattta acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc
     6781 gatcattgtt ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt
     6841 agcttcaaat ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat
     6901 ggtcctattg tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat
     6961 gttccatatt atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg
     7021 tactttgaca acggccattg ctccagcaat tggccccact tatggaggca ttatgacttc
     7081 acattttaca tggaatcata tcttcctgtt tcttgtgccc gttttgtttc tttctttgat
     7141 tatcggctgg tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat
     7201 aagcgtttta ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg
     7261 aacagtttcc agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta
     7321 tcgaagtaaa aaagtggagc atccactgat ccgactgtct attctttcaa ataaaacatt
     7381 ccgcttgttt ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt
     7441 cttgccgaat ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat
     7501 gcttcccggc gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca
     7561 atatggtccg aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc
     7621 attattccta ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta
     7681 tatgatcggt atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa
     7741 tgaagaacta caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc
     7801 tgtggctact tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa
     7861 ctatgaagtg tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct
     7921 tctcgtaagc tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc
     7981 atatacggca ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga
     8041 ggctttttta tggaaaaaat tgccattatc ggtggcggga tcattggtat gaccctagcc
     8101 agttacttag atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca
     8161 actaaagcta gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg
     8221 tatcgtctgg ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt
     8281 gatcaaacga tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct
     8341 gaattggctg ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa
     8401 tggctttcac ctgaacagac atctcaagca cttccgttat tgaaaccagc agtttcactg
     8461 cgtatcagtg gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt
     8521 ttagcacaaa aacaaatgga gcagatccag gcaaaagtca ctttgcataa gaagagcgat
     8581 tcttggctca tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct
     8641 ggcccggcac ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa
     8701 aaagggcagc tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct
     8761 atgcttgacg gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct
     8821 acccatgaaa acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg
     8881 actaagagta cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag
     8941 ttccgtgtag gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca
     9001 gatgatccaa gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct
     9061 tatatcggct atctattagc caacttctta aatactggtc attggcctac ggaagattat
     9121 caaaaggaca tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag
     9181 taaggatccc gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc
     9241 aatttctgaa tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct
     9301 atctgagctt caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag
     9361 gacgtatcct caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat
     9421 ggaccaaacg acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc
     9481 gtttcaggtt tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt
     9541 tgctgtttca aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg
     9601 aaaggattag caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt
     9661 tccgcgtctg atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aactgggtca
     9721 ttgatcttga taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc
     9781 ttttcgctaa gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca
     9841 tttccgtttc gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg
     9901 cccacattct gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt
     9961 gttttatcaa agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg
    10021 aaagcatcgt catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc
    10081 gttgattttc cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta
    10141 gcatattgtt tcatcccacc gtggcaggca ataaagccat tgcgatttcc caatgcccaa
    10201 gcaagcgtca aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacagccgct
    10261 gtattatgga gagcatcaaa caaaatgagt ccatttggaa atgtttcatg tgtccattcg
    10321 ggatcacaaa agatataaag atctccctca tcaaaggaaa cagacttttg ataagtctct
    10381 aagatcttcg aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat
    10441 ccttctggaa tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact
    10501 tttgtatggt aaaactggcg cttgcgactt tgatacacag cttctcttaa aattttctcg
    10561 tatttgcttt gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc
    10621 acaatgcttc catcattgaa tactagtgtc ctaacatcag ctggtaaatc tagttcttca
    10681 gcatgtgaga ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa
    10741 gctgtcttcc tatcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc
    10801 gatcgaatac tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct
    10861 tttgtactca tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt
    10921 attcgagttt ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa
    10981 aaaaaccgag aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt
    11041 agtaccagcc gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg
    11101 ttacatattg ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg
    11161 aagcatctaa ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg
    11221 attctttttg tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcagctg
    11281 gggcagcagg agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg
    11341 ctgtttcagt tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat
    11401 catttgaagt tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg
    11461 aatttttttc agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct
    11521 tagataatga atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc
    11581 ctacaaacat tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg
    11641 aattcatatt aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat
    11701 catttagaaa tatttcatag gtaaaagaaa catgacattt aatgacaaaa taacgaatta
    11761 tttaatattt gtaacattta aacaaatatt tttttgatac aaaaccataa aagccttgtt
    11821 gtataagcaa agtaatcgtt tttctatgtt tttttatttt tgaatgaaaa acgcagattc
    11881 tgtaacattc actgcaataa tccataaaaa aaagcaaaaa aatttttgat ttttatcaaa
    11941 ttctcttaaa aaaatgtaaa aaatacaaaa aaaccagtaa ttgttacaac aattactgga
    12001 caaaaacttt gaataatgtt aactattttt ttattttaac tttctccgca ataatttctg
    12061 ggcatgttcc aattcgttta taacgacttc ttctgtttct ttagctaata tttcatctaa
    12121 tacatcaagc catttttccg gttcttttgt tcctagacgt ccaatagccc aagctgctgt
    12181 tcctctgatg acagggcgga catctgttaa acattctgaa atctcaggca atgccgttcg
    12241 atcacctaga ttagctaaag caatcaatgc gtttctctgc aaaggtttct tcccacgcca
    12301 tgaaccagct aaatgcccaa attgctgttt gaagtcttta ttagacatcg aaagcattgg
    12361 ctttaatttt ggatatattt cgtctacttt tggttccatc tcttcatgaa aatgaaaatc
    12421 ttttccttga ttgtaagggc agactaactg gcagatatca catccataaa tgacatttcg
    12481 cattttttta cgatattctt ccggcatcat cccttttgtc tgagtttgat aagacaaaca
    12541 tcgctgggca ttcattcttc cgtcaccaag caatgctttt gtcggacaag cagtcacgca
    12601 gcgcatacag tcaccgcaac caaattctcc tggtttatca ggctcaaata caatattagt
    12661 aacgatctca ccaagataga caaatgaacc gaactcttca gtgatcaaca atccgtttcg
    12721 tccaataaat cctaaaccag ctcgctgtgc tacagcgaca tcgatcagtt ctccagtatc
    12781 tacttgcgga gctagacgcc attcctcttc tttttgccat ttttctgctc ttgtttggat
    12841 gaattcgatc aatcggttca aacggtctct gagtatatca tggtaatcga tcccccaaga
    12901 agcacgagca aactgcccgc gtttttcatc cctaggcact tcttcatgga tcctagtagg
    12961 atacgcaagt gcaatggaaa tgatcgattt cggctgatca aatgtcattt ctggataaag
    13021 acgctcatcg atatttggat gttcaaagcc agaagtatgg ccagctgctt tttgttcttc
    13081 caacgattct tttaatgaat caaaaggttc cgccgtggtg aacccgattt tgtcaattcc
    13141 taatcgtttg ctttcttgta tgatttcttc ttttaatgtc atggtcttcc tcgcttgctg
    13201 ttaacggctg tataacccat aatatagttt ttcttttttc agctggacaa actggatctg
    13261 ctgatcggca atcttctttt cttccatcca tttttttctt gcagcaaata cttcccaata
    13321 atttgttgaa attaattttc ttttgttatc taatgcatta aaaaaattca atgaaactgg
    13381 ttttgtactt aacagttcat ggagccttac ctcttcccat ggcgaaaact cagacagatc
    13441 gcgggaatac aatacttctt catagagatt tttttgctgt gtgtacgtga atgtttgcgg
    13501 gattttccca ggcctagcaa tttctactag acgattataa aacatattca ttaatttgct
    13561 gttggcaata aattgacttt tataataggc cgactggatc tttttgatta ctccttctgc
    13621 tttgatgact gaaaataatg caggccatgc tttgacggtt atttcgtcgt tttgtttaac
    13681 taatacgcca ggtgtgaagc cgactggaga aaggaacaca aaataaccga taccggtatt
    13741 ttcatctaaa ggctgaaatt catcaaacaa ccatttgcct atgtacagct cttcatctgg
    13801 ttctgttgtc tcattttgat ttgctatgaa taatttagct aattctgctt caagaaattg
    13861 aaggtcttga tccttgtcgg tattttcctg ctggctgatc aatcgaaaga cttcttcttt
    13921 aatcgtatat ttatctttac gtgtaaaatt catagccaca ccttccttcc ttactctaac
    13981 tataacggaa tttcttcaaa aaaggtatct gtacgacgaa aaacagcata attttttttc
    14041 atatctctta taaaaaatgc aaggtctgct cctcaagcta gaccttgcac ttttcagctt
    14101 tcttttgttt gtgacaaacc ttcatggatc gtttcaatat tccatttcat catctgatag
    14161 taagaatctc cttgttctcc ttcttttgct atggaatcag tgaacagttt atcatagatt
    14221 ttcaaacccg tttcttttgc tacccgttcc atgcttcttg agtctacact ggtttccacg
    14281 aataaaaccg gtacttcttt tgctcttatc tgatcaatga tcgctttcat ttgatcaggc
    14341 gtaccttgac tttctgtatt gatctcccat atataagctg ctggcaaatc atatgctcgt
    14401 gaaaaatact tgaatgcccc ttcacttgtt actaataatt ttttgttctc ttcgatagaa
    14461 gcaaaacttt ccttcgcttg tgtatctaat tcttttagtt tttccacata tgcttctgca
    14521 ttttttttat agagtgtcgc attttctgca tctttttcag agaatatacg tgcgatttcc
    14581 tccacatatt ttattccgtt agataggtct agccaagcat ggggatctgc ttttgtatgt
    14641 tcttcaccgc tagttaaata aagaggttct acatttttgc taactgcaaa ataatctttt
    14701 ccttcttctt ttttagccgt ttccatcaag ttatcgaacc agctgttacc tgtttcaaga
    14761 ttcaaaccgt tgtataaaat aacatctgca tcgcttgcct ttttgatgtc ttctggtagt
    14821 acttcatatt catgcggatc tgttccgact ggtacgatac tgtggatatc tatttgatct
    14881 gttccgacct cttttgccat gtccgccaag atcgaattgg tagccacgac tgacaacttt
    14941 tctttacttc cttcatccgc ttggtcagaa gcctgctttc cgcaaccggc taataccaag
    15001 aaactaactg ccaagattaa aaagagtgat ttttttgttt tcatctatct gcttcctttc
    15061 tgaaataaaa ttccttttgt tggagaaaat aaaaaagcaa ggataaaaaa tgcggtacat
    15121 gctaacacga tcgtagctcc tgatggccaa ttgtaactat aactgaaaaa cacaccgaca
    15181 actgcactta gcataccgat tcctgcagct gtaatgatca ttttcagtaa atggttcgtt
    15241 agcaagtaag ccgtggcggc tggtgtgatc aacatggcaa tcaccaagat tgttcctact
    15301 gtctgtaaac tgactacagc cactaatgtc aaaaagaaca tcaaaagata atggatcagc
    15361 gatgtgttca gcccataagc ttttgccatt gtcggatcga atgatgtgat cttcaattct
    15421 ttataaaaga aaaagacaaa tatcaagacg attccactaa cgagtgctgt cagcaacaaa
    15481 tcgctttccc taacagctaa gacattccca aataaaatat gatacaaatc tgtcgcactc
    15541 tgggcatatg aaatcaagat aatccctaat gcaaaaaagg agctaaagac aattccaatc
    15601 gctgtatcat ttttcaatcg gctccgctga gtcacgaaac caatcactgc tgctgttagc
    15661 atcccaaaag cagtggctcc aaagatgtaa ctaaaaccaa acatataaga aatggctaca
    15721 cctggaagaa ctgcgtgaga gatagcgtct cccatcaaag acatccctcg taaaataata
    15781 aaagaaccga tgactccaga agcaagtcct acaagcatgg aagtgaccaa agcgtattgc
    15841 aggaattgat aattcattaa tccatcaata aaggattgga tcattttttc tctcctcctt
    15901 ttactaaatg tctgatgatc tcaccatagg ccaattgaag gttcttcgat gtaaagactt
    15961 cttgtactgg accggaagcg atcagctgtt tatttaaaag aataacctca tcgaaatatt
    16021 cttctacttt atgaagatca tgatggacga tcaaaatcgt ttttcctgaa ttcttcaatt
    16081 cctgcaagat gtcaaagatc tttcgttcac ttaacgcatc aatccctacg aatggttcat
    16141 ctaaaaagat ccattctgct ccttgggcta gagctcttgc aataaaaact ctctgaagct
    16201 gtccacccga tagttcgctg atctgtcttt tggcatattc ttccatccct acttttttca
    16261 aagcttgtct tgcacgttct ttttcttttt tcccaggtct ttgtccaatt cgtaaagatg
    16321 gatatgttcc taaaagtaca acgcctaata catcaattgg aaaggaaaga tccaattcac
    16381 ttcgctgttc cacgtaggct atttttttac ggactgagtt taccgactgt ccagaaaagg
    16441 tgactttacc agtagtagct ggtatcaact ctagcaaaga tttcataaat gttgattttc
    16501 ctgcaccgtt tggtccgata ataccggtaa ttttttgtgg atttatcgtc aagttgatag
    16561 ttttcaaaac agttttccct tgataagcgg ctgaaaggtc ttgaattaca atggccgtac
    16621 gttccaaatc ttacactcct ttttaggttt acctaacttt ttatttaaat atagtttaac
    16681 taaaatggaa agcattttca agtagaatga tgaaaaagtt tttttcgaat tttcctcgca
    16741 ttttatgaaa attttctgta gaatcacatc aagagttcaa taaggctaac ctaataatga
    16801 aaaacatgtg ttgaaacgtt tgattatttt ttcttgttga ctcaacaacg aaaggagttt
    16861 ttgtatggaa gaagaaaagt taactcgaag tcttagtgcc cgtcatatcc aaatgatcgc
    16921 actaggtgga acgatcggtg tcggtctgtt tatgggtgcc tcctcaacca tacgttggac
    16981 agggccctca gtgatgggag cctatgcgat tgcaggattc tttttgtatt tgattatgcg
    17041 tgctttaggc gaaatgcttt acgttgatcc ttcgactggt tcttttgcaa attacgcgag
    17101 cgagtacatc catcctgtgg ctggctactt gaccgcttgg agtaatattt ttcaatatat
    17161 tgtcgtagga ataagtgaag tgattgctgt aggatcttat atgaattatt ggtggcctga
    17221 tttgccggca attattcccg gaatcattgt cgtcttgttt cttatgctgg caaatcttat
    17281 ttctgtcaag gcgtttggcg aattagaatt ttggttttcg atcatcaaag tcataacgat
    17341 cattttgatg atcattgcag gtcttggggt aatcttattt ggatttggta atcatggtca
    17401 ggcagtaggg atttcaaatc tttggaaaaa tggtggattc ttcactggag gcgtcaaagg
    17461 atttttcttt gcattatcga tcgttgttgc ttcctatcaa ggaatcgaac tgatcggaat
    17521 gactgctggt gaagcagaga atcctaaaaa gacgatcatt gaagctgtcc agtccactat
    17581 cggtcgaatc ttgatttttt atattggtgc tatttttatc attgtgagta tctatccgtg
    17641 gaatcaattg agtgagattg gttcaccttt tgtacaaact ttttctaaag tcggcattac
    17701 atttgctgca ggactgatta attttgtcgt gattacagcg gcattatcag gttgcaattc
    17761 agggattttc agcgcaagtc gtatggtcta tacacttgcc ataaatggaa agatgtctaa
    17821 aaaatttctg aaactatctc gtcacggtgc gcctttttat cctgtcgtag caatctcagg
    17881 tggaatctta attggtctga tcttaaatta tcttctacca atgttcatca accagtcaga
    17941 aaatattttc gttttcgtat acagctcaag tattttgcct ggtatggttc cttgggtagt
    18001 gattttgatc agcgagattc gcttcaaaaa gaagcacgca gataaaatgc atgatcatcc
    18061 ttttaaaatg ccactatctc cttatacgaa ttatgtgaca cttgcttttt tagctatcgt
    18121 attgattttc atgtttatca atcctgaaac gagaatctca ttgattattg gactcgtttt
    18181 cttgctttac atgacgatcc attactttgt acgcgaaaaa agaaccggaa aataaaaaaa
    18241 gaacgaagct tcaccttagt ggaagcttcg ttctttttta ttgcttttat caattcaatt
    18301 ttagcgtctc accgggagtc aggaaataat tctccatgct catcccattc agcgacaaga
    18361 tcgtttctac tggtacgcca gttcgttcgg ctatttgatt tggaccttct ccttccaaaa
    18421 ctacggttgt cgcagcctgt gaagtcgcgc tgttggtatt agtttcagag gaatctgttg
    18481 tgtcttgtgt tgttcgagct tgcatttcag aagcttctac cgactctgct gtctcagaat
    18541 cagaagatgt tgtgtcttgt gtgcttgttg actctgcttg aagagaggcg ctagtctgca
    18601 aataaatatc ggaaatctta gcgacatgat tcgtataatt agccgataat tgattgatca
    18661 aagtatctaa gtctacaccg tctttatttt ctgctgcctg atacgctgca cgtaattttc
    18721 tgatcccttc gtcggagatt gcttgaagct cacgtgcttt ttggttagca actgctgata
    18781 aaccagctat tccctcttga tttccctgaa tttctgtctg gtattcttca atcaatgcag
    18841 gcgtcttttc ttcgatcttt ttcgaataat catttacgat tgcttgaagt tgctctggca
    18901 aaccatttgc tgttttttct tcagaagttt gtgtctcttt tgttttagtt gatgactcac
    18961 ttgtttgttt cgtctctgtt ttctttgtcg ttgtttccgt tatggtcgta cttttcgctt
    19021 ctgacgaatt ttttccatga aaatatagac ccgcaactcc tcctgctatg atcaaaaaca
    19081 cggcaactgc tgccccaatc atgatcatcc gcttacgagc tgcctgtttt tgctgtcttc
    19141 ttcgggtcat ccgaacattt tctccccact cattttcatc atacattttt ttcaccacca
    19201 atttttatgt ataatagcgt tatcaaacgc ttcatattct tattgtagca aataattgtc
    19261 gctgtgcaca tttttatctt tgttatttat aagaaaattt caccagttga gatgattcca
    19321 agattgtccg tcgatttctc cttgcagctg cattgttcgt tcgtctatct cagccatcag
    19381 tttctttatt tcatataaga gcgcacggtc ttgatattct gatttttcgc ttaattcatc
    19441 taattgttgg atcagccaat catttgtcat aatgaagttc ctttcatttc ttgcagtgta
    19501 aaggtcaagt cttcgatctg ctcttttgcc tcagtcagtg cttctacgat atatgcttgt
    19561 ttttcagatt cttcttcctg cattttctga ttttcctgta ctctttgaat aaaccagtca
    19621 atccgctgag tcagcgatgg tttgccttcc aatgagagat actgctgata agcaattagc
    19681 ttctcccaac gtttttcctg ggataaatac ataaattgtg ctaaaacgaa tggcggaaga
    19741 taggtcatat ggaatttatt tgcgattcgc tgatacggcc tcaaaaattc ggaaatcgta
    19801 tattcttctt ttcctccagc ttggtattct ttttccgcca cacctgttgt caatacgatt
    19861 cctagttctt tcccttttaa tacatgtgct gctcgttcca agacattatc ttgccaaatt
    19921 tttaaatgag caggagaact ataccagtaa aacggaaact ggaaaatgat ccgatcatgc
    19981 tgggccaaga gctgtagttc agtctctata tctatttttc cgtctggata acttgattcc
    20041 aaatgatgcc aagtagctgg taaaccagca gaagcttctt tgaaaaattg ctgatttaaa
    20101 gattcttcga tcgttggatg gctaatgatg ataagtgtac gcatcccttt tcctcctttt
    20161 tagtcttcgc tttacagtgt agcataaccg ccacaagttt cttttgccga tgcttgtcat
    20221 tttaaccaga ctaatcaggt tgtccaagaa actgcggttt gtttttccaa ctgttttttc
    20281 aaggtatcca aagcaatatt cttaaaattt tcgtttttca tacgtttacg atagttcgta
    20341 gccgtttctc cggtactctt tttgaacatt cggctaaata cgactagact attgaaccct
    20401 acttgatagg caatactttc cacggattca tccgtcagag aaagaagaaa cgcccctcga
    20461 ttgatccgta ccccgatcaa atattccttg atactggtac ccatatgatt tttgaatact
    20521 cgcgacaaat ggctccgatt gaccgctaaa tgctgggcga tttcctcaac agacaatttt
    20581 tcagcataat gtgcctcgat gtatttgact gtttcaacag ctaaagagga atgaaccttt
    20641 tgtacaccac cgctgacctt tccgccatct tttaaaagga ctgataaaaa acggtaggtt
    20701 aatgctgtta actgtaattc attttccaag gtttcttccg tatctttcat gcattctaaa
    20761 ataatgtcga tatatttttg tatgtcagaa gagaccatcg tgtattggtc ttctctgaaa
    20821 agcgactgcc gaatgatttc ttctgcagca ctcccaccaa atgaaagcca gcaatacatc
    20881 cacggatctt ctccatctga taagtaaaac gtcgagtctc ctggacgaat caagaacaga
    20941 tcaccttttt tcaattggaa ttgttgattt ttcacatgat aagatccttt gccttccatc
    21001 acgacatgca ggatgtaatc attgcgaata gcaggaccaa aagaatgaaa cggcatcgtt
    21061 ttagagaaac cacaaaaatc aaaatacaat tctttttcta cttgttgatg ttcctttttt
    21121 aaaaaaatcg attgctccac gtctatccct ccttactcct ttagtataat gcaacaaaaa
    21181 gttaaataaa agtcacagaa atatattaaa aaggaagcga tttcttgtta agtttaagtc
    21241 attaaaagat ctttgttatt cgttgtaacc ggtttactta gtattatata ttgaacagga
    21301 gaggaataca gattatgaaa agcaaaatga tccaacgtgt gtcctacgct ttaggcgctt
    21361 ttggacacga cacattctat gttacattaa gcacttattt tatggtattc gtgacaagcc
    21421 agatgttcgc aggaacagat aaggcaacga atgcaaaaat gattgctaca gttacaagct
    21481 tagttgtagg tatccgctta gttgaaatta tttttgatcc tttgattggt ggcatcatcg
    21541 ataatactcg tacaaaatac ggtaaattca aaccatggtt agttatcggc ggcgttgtta
    21601 gttccgtact cttggtagca atctttacca atttctttgg cttagctact cataatacga
    21661 ctttatttat catattattt gcaatcgttt tcatcttatt ggatagtttt tattcattta
    21721 aggatatcgc tttttggtca atgattccag ctttgagtac ggaaacaaag gaacgtgaaa
    21781 aatttgctac gtttgctcgt ttcggctctt ctctaggtgc aaatggtact actctagtcg
    21841 ttgtccctat cgttactttc tttacctatc ttgttactca taaacatatt gaaggcgctt
    21901 caggctggtt ctggttcggt gtgattgctg ctgttatttc aggcggtact gcgattatca
    21961 ctgccttggg cacaaaagaa gtcgatactg cgatccgtca gcaaaatgaa aaagctggga
    22021 tcaaagatgt gttcaaagca gttgctcaaa atgatcagtt gatgtggctt gcccttgctt
    22081 atatcgctta tgcgattgcc aatgcagcaa caactgccgt attattctac ttcttcaaat
    22141 atgtacttgg ccaaccaact gaattttgga tcgttggtct agttgctgcc ttacttggtt
    22201 tagtagctgt tccactattc ccaatgctta cacgcgtcat ctctcgtcgc tacgtttacc
    22261 ttggagggat cgcaatgatg atttcttctt actttttctt tacgatcgca ggttcaagtt
    22321 tgccaatcgt tatcatcggg ctagtattgt tctacttccc tcaacaattg attttcttat
    22381 ccgctttgat gacgatcaca gactctgttg aatatggtca atggaaaaat ggtatccgta
    22441 acgaagctgt tactctatca attcgccctt tactagataa aatcgctggt gctttgtcta
    22501 acgggatcgt agggtttgtc gctatcgctt ctgggatgac tggtaatgct actgctgctg
    22561 atatcactgc tcatggcgta acgactttca aagcttatgc attctatgct cctgctgctt
    22621 tgatgatttt ctccgctatt atttttgctt ggaagattac attgacagag aaaaaacatg
    22681 cacaaatcgt tgagaaattg gaaaacaaat tggctccagc tgatacaatg gaagatgaac
    22741 ttcacgatgc agtagatcct tcccattaag aaaggatctt gaaaatgaag attacagaaa
    22801 aagattttgg ccaaggttat cacttgataa ccttggagaa caaaaacaag ctagcacttt
    22861 ctatctctga tttaggtgct agaatcgtca gtttaaaatc aaatgatcga gaattagttt
    22921 tgggatttga caccgctgaa gaatatatcg aaaaagatcc gtatatcggt gcttctatcg
    22981 gtcgcaccgc agggagaatc gaaaatggcc gcttttctct caatggaaaa acttaccaat
    23041 tagccacaga tccaaaaact ggacataatc tgcatggtgg tgctcctgga tttgaattaa
    23101 aaaaatggtc ttatgtcatt ttgaatggcg aaaatgaagc ttctgtcatt tttactacta
    23161 caagtcccga cggagaacat ggttttccag gtacaatgga tgtggaaatc cgctatactt
    23221 tgacaaaaga taatatctgg cgagtgacct ctcgcggcac cagtgaccaa gatactttat
    23281 tcaatccgac aaatcatgtt tacttcaact tgacgggaga tgcttctcag tcaatcgatc
    23341 aacatgagct ttggctcaat agcgaagctt atgctccttt gcgaacagat agtattccta
    23401 taggggtaaa agaaaatgct gcaggaagtg ctttcgattt tcaaatacct aaaaaattag
    23461 cgtccgtttt tgctagtgat ttggatcaaa aaaatcttgt tgacggaatc gatcatccat
    23521 ttttcttaaa agaaaccggc ttgggtaaag aagccgctcg cttgactagc cccgataaaa
    23581 gaatccaagt agatattgct acagatgctt ctagtgtggt tattttcact gcaaatttcg
    23641 gcacagaaac accagaaatg cgcaaccgaa aactggcaca tcacggtggc atcacatttg
    23701 agactcaaac agctccaggt gcagagcgct tctcagcatt tggttcgatt catttaaaag
    23761 ccggatcagt atttgaaaca gtaacagaat tcaaaatcaa aacaagaaag gaataactca
    23821 tcatggaaat caaaagaagc ttaaacacaa aatttgccga aacattcgga cctaaaaaaa
    23881 ctacacaata cttctcacca ggacgcatca atttgattgg tgaacacaca gattacaatg
    23941 gaggccatgt cttccccgct tccatcactt atggaacgca aggtgtagca gcacctcgag
    24001 acgataataa aatattagtt tattccacaa actttgacga tatcggtgtc attagtttta
    24061 ctttagatga gctagaatat gataaaaaag caaactgggc aaattacgta aaaggaatgg
    24121 tccttaaatt aaaagaagct ggacactcaa tcgatcatgg gtttgagtta ttagtggaag
    24181 gaacaatccc taatggtgca ggactttcca gtagcgcttc attagaatta ttagtaggtg
    24241 ttgtattaga agatctgttt gatttaaaaa cagatcgctt ggaactagtc caaacaggta
    24301 aaaaagtaga aaatgaattc atcggtgtca attcaggtat catggatcag tttgccatcg
    24361 gcttcggtga gctagatcat gcaatcttat tagatacaaa tacgctgaaa tacgaaatgg
    24421 ttccagtgaa actagatggt tatgcaatcg tcatcatgaa tacgaataag cggcgggaat
    24481 tggcagattc aaaatacaat gaacgccgta gcgaatgcga agaagccttg gcacgtttgc
    24541 aaaaaacttt gcctatctct tctttaggag acttagatga agaagaattt gaatcaacta
    24601 ttgaccaaat cggcgatgat acattgatac gcagagctcg ccatgccgtt tctgaaaatc
    24661 agcggacttt gaaggcaaaa gctgaattag aagctgggaa cttagaagca tttggtcaac
    24721 tgttaaatga ttctcaccac tctcttcgct atgattatga agtaactggg atcgaattag
    24781 atacactagt agatgctgct caaaaacaag aaggtgtctt aggtgcacga atgacaggag
    24841 ctggatttgg tggctgtgcg attgctcttg tcaaagaaga aaatatacct gccttcaaaa
    24901 acaatgtcta tgatgaatac atgaaagttg tcggctatgc tccggaattt tatgttgcac
    24961 acattggaaa tgggacaaca aagatcgatc cagaagctgt tcatgtttga taaaaaaatc
    25021 aacgatctca gctaaaaaca aatagataaa aataacctct cagcaatttc caaatgtgag
    25081 ccttttgctc gtatttgaaa attgctgttt tttattgaca cggaaagaga acgtgtgttc
    25141 taattgtgtt gaagaggtga ttgttatgtg ggcgaatcaa aatccggtat ttgtttatga
    25201 aaaagaacct tcaagagata ttttatgtat tgactgcaaa tctttttacg cctccgtaga
    25261 gtgcgttgaa aaggatcttg atccccttac aactaaatta gtagtcatgt cctatccgtc
    25321 cgattcgaca gaaacacggg gtagcggatt aatcttggct tccagtcctg cagcaaaaaa
    25381 agcttacggc atcaccaata tcagccgtgc cagagatctt cccttcccct atccaagtga
    25441 tttatatatt gttgccccaa ggatggccta ttatatggaa aagaacagag aaatcaattc
    25501 tatctataaa aaatatgcag acgaagcgaa ccatcatgtc tattccgtcg atgaaagctt
    25561 ccttgatgta acaaatagca tggcactctt tggtaaaaca aatgcgtacg acatggctaa
    25621 actgatccat aaagatgtgt ttgagcagac cggaatctac acgactatag gaattggcga
    25681 taacccgcta cttgctaaac tagccttaga caatggctca aaagacggaa agcaaatgtt
    25741 tgctgaatgg cgttatgaag atgtaccaga aaagttatgg aaaatctcag atatcactga
    25801 cttttgggga atcggcaaac gaacagcaaa acgtctgaaa caaatgggta tacattctgt
    25861 ttatgattta gctcacgcgg attattatca cttgaaaagc aagctagggg tcatcggtac
    25921 tcagctttat gcccatgctt ggggcatcga tcgaagtttt ttgggacaag aatatacacc
    25981 aaaatcaaaa agtatcggaa atagtcaaat cctagataga gattatacaa gacgagaaga
    26041 aatcgagatc gttatcaaag aaatggccga ccaagtcggc actcgcttga gacgatctgc
    26101 agcaaaaaca caagtagtca gcttatgggt aggatactct ttaggctatc ttgatccaaa
    26161 tggcaagacc gggtttcacc agcaaatgaa aatcgaagcg acaaatgcta gcagagatct
    26221 agcagatgct ttattgacca ttttccacca gcattaccac taccaggata ttcggcgtgt
    26281 aggcgtgaac tgttccgacc tcgtatacgc gacaggttta cagcttaacc tttttgaaga
    26341 tcctgaaaag cagataaatg aagcgaaagt tgattttgtt gtcgatacga ttagaaaaaa
    26401 atacggcttt caatcaatca tccatgcgca tagtctcttg caaggaggac gcgccatcgc
    26461 acgtagttct ctcgttggcg ggcatgcagg tggtatgagt ggattagaag gtggaaaaga
    26521 tgagacggac agcaaaaaaa ttcattgact ataacgagta tcgagatcgg ccatttggat
    26581 taaaatgggg gactgctttt gcgatggaag aactagtcaa cggtatcaaa aaaatgaaga
    26641 agaagcacgc aaacagcctc ttcctaaaca acaaatgaca ttgcaagaaa tcgatagtat
    26701 actgacagaa gcctttttat ctcgtaaagc agtaatcatt cagctcaatt taaaagatga
    26761 atttggaaga ctcttggatg aggtcgaagg gcacttcatt ggtgaagcct atgaagatta
    26821 ctttatgatt gaccatcagc cgatattgtg ggaagacgta cggtatattg agttaaagga
    26881 aacaaaaaaa tggtttgtga catgagaggt tgtgacagaa gtgatcaact tcaaaaaata
    26941 agaaaggatt tttcgaaaat tgcttcttaa atttttgtgg aattttggct tatttttcga
    27001 agaagcaact tctggaacaa cgtatattag atatcagagg ttgtgaaaaa gtcgcttagt
    27061 tttgagtaac aagccaaggc gctgatttca caacctctgt ctatctatcc atctattcgg
    27121 taagattcat gcatgacgtc ttctttattt agtatgtaga gccctttttc ttctgaccat
    27181 tcaacataaa tcagctgttt tacatctgca tagccttctt tcttcaagtt ctccataagc
    27241 catgcttcag tcaattgatt ggactgaagc actttatgct gaatttgccc ttcatctaca
    27301 agcaggatcg ataaagcttt gtcttcatct gattttttca atacactcat ttgaccggca
    27361 ttctctagga taacatgttt ggcattttca agaaaaaagc attcttgctg tcttaagatt
    27421 gctcgcaact gctccatctc gatatggtta tttgtcaact ctgtcatgtt gatgacgccg
    27481 tctttgatta gaacagatgg ctcgcctttg acccaacgat tgaccttttc atttttttgg
    27541 acgatacgtt cgataccata aatcattact gcccaaagcg caatggcaaa taggacatgc
    27601 cctacatgga cattgtcatc ataggttgat tcttctaaga tcccacctaa aacaagcgta
    27661 tagactaaat caaacggtgt gatttctgac atggatttct tcccaagcaa acgtaccact
    27721 aataataaac cgatcaaccc gaacaccagt ttcattgtaa ttgaaaaaat cattttttct
    27781 ccccctaact atctgttaat taaattatat atgctttctc acgaacacgc atggaatata
    27841 caaggaagaa atgtatcctt ttactaaaaa aaggactgtg acactgtagt gacccccaaa
    27901 agttagactt ttttggagtg gagaaatcca ctcctttttt ttatgctc
//
LOCUS       DBNICI010000018        26497 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.018, whole genome shotgun sequence.
ACCESSION   DBNICI010000018 DBNICI010000000
VERSION     DBNICI010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.018"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(15..734)
                     /locus_tag="AC1ZCC_000648"
     CDS             complement(15..734)
                     /locus_tag="AC1ZCC_000648"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIQ1628921.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    924..971
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            1071..1403
                     /locus_tag="AC1ZCC_000649"
     CDS             1071..1403
                     /locus_tag="AC1ZCC_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628922.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            1390..3393
                     /locus_tag="AC1ZCC_000650"
     CDS             1390..3393
                     /locus_tag="AC1ZCC_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HIQ1628923.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            3412..3882
                     /locus_tag="AC1ZCC_000651"
     CDS             3412..3882
                     /locus_tag="AC1ZCC_000651"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HIQ1628924.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            3903..4487
                     /locus_tag="AC1ZCC_000652"
     CDS             3903..4487
                     /locus_tag="AC1ZCC_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HIQ1628925.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            4502..5488
                     /locus_tag="AC1ZCC_000653"
     CDS             4502..5488
                     /locus_tag="AC1ZCC_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HIQ1628926.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            5478..5789
                     /locus_tag="AC1ZCC_000654"
     CDS             5478..5789
                     /locus_tag="AC1ZCC_000654"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HIQ1628927.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            5811..7592
                     /locus_tag="AC1ZCC_000655"
     CDS             5811..7592
                     /locus_tag="AC1ZCC_000655"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HIQ1628928.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            7585..8961
                     /locus_tag="AC1ZCC_000656"
     CDS             7585..8961
                     /locus_tag="AC1ZCC_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HIQ1628929.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            8968..9603
                     /locus_tag="AC1ZCC_000657"
     CDS             8968..9603
                     /locus_tag="AC1ZCC_000657"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HIQ1628930.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            9633..10988
                     /locus_tag="AC1ZCC_000658"
     CDS             9633..10988
                     /locus_tag="AC1ZCC_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIQ1628931.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(11180..12574)
                     /locus_tag="AC1ZCC_000659"
     CDS             complement(11180..12574)
                     /locus_tag="AC1ZCC_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HIQ1628932.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            12666..13637
                     /locus_tag="AC1ZCC_000660"
     CDS             12666..13637
                     /locus_tag="AC1ZCC_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HIQ1628933.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            13634..14416
                     /locus_tag="AC1ZCC_000661"
     CDS             13634..14416
                     /locus_tag="AC1ZCC_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HIQ1628934.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(14585..14956)
                     /gene="gloA"
                     /locus_tag="AC1ZCC_000662"
                     /gene_synonym="lguL"
     CDS             complement(14585..14956)
                     /gene="gloA"
                     /locus_tag="AC1ZCC_000662"
                     /gene_synonym="lguL"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HIQ1628935.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            15090..15533
                     /locus_tag="AC1ZCC_000663"
     CDS             15090..15533
                     /locus_tag="AC1ZCC_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIQ1628936.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(15576..16385)
                     /gene="yidA"
                     /locus_tag="AC1ZCC_000664"
     CDS             complement(15576..16385)
                     /gene="yidA"
                     /locus_tag="AC1ZCC_000664"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HIQ1628937.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(16404..17771)
                     /locus_tag="AC1ZCC_000665"
     CDS             complement(16404..17771)
                     /locus_tag="AC1ZCC_000665"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIQ1628938.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(17755..18585)
                     /locus_tag="AC1ZCC_000666"
     CDS             complement(17755..18585)
                     /locus_tag="AC1ZCC_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HIQ1628939.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            18748..19185
                     /locus_tag="AC1ZCC_000667"
     CDS             18748..19185
                     /locus_tag="AC1ZCC_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HIQ1628940.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            19261..19881
                     /gene="rpoE"
                     /locus_tag="AC1ZCC_000668"
     CDS             19261..19881
                     /gene="rpoE"
                     /locus_tag="AC1ZCC_000668"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HIQ1628941.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            20070..20576
                     /locus_tag="AC1ZCC_000669"
     CDS             20070..20576
                     /locus_tag="AC1ZCC_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIQ1628942.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            20593..21492
                     /locus_tag="AC1ZCC_000670"
     CDS             20593..21492
                     /locus_tag="AC1ZCC_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HIQ1628943.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            21579..22463
                     /locus_tag="AC1ZCC_000671"
     CDS             21579..22463
                     /locus_tag="AC1ZCC_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIQ1628944.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(22655..22726)
                     /locus_tag="AC1ZCC_000672"
     tRNA            complement(22655..22726)
                     /locus_tag="AC1ZCC_000672"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22691..22693),aa:Arg,seq:ccg)
     gene            22786..24120
                     /gene="rpoN"
                     /locus_tag="AC1ZCC_000673"
     CDS             22786..24120
                     /gene="rpoN"
                     /locus_tag="AC1ZCC_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HIQ1628945.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            24269..25360
                     /locus_tag="AC1ZCC_000674"
     CDS             24269..25360
                     /locus_tag="AC1ZCC_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HIQ1628946.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            25401..26402
                     /gene="gap"
                     /locus_tag="AC1ZCC_000675"
     CDS             25401..26402
                     /gene="gap"
                     /locus_tag="AC1ZCC_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIQ1628947.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
BASE COUNT         8641 a         4624 c         5692 g         7540 t
ORIGIN      
        1 cctcagaaag tctattaaaa ttcattcaca aagaaatcga ataagcgctg ttcagatgga
       61 tgtacacggt gggtaagttc cggatgccat tgaacaccga ggattttatg catatcatct
      121 ttgctttgaa ccgcttcaat gagtccatcg ttagaaaaag cgatggcttt taataaaggt
      181 gaaagatctt tgactgcctg atgatggtaa gaattcacaa aatatggatc ggggagaagg
      241 ttgcttaaca aactgtctga aacaatcttg acttcgtgag tcgtaaattg cggggcagtg
      301 ggctgttgtt catgttttac tgaccacttt ggatataatg aaagatcttg ataaagtgta
      361 ccttccaaaa cgacattcaa tagttggagt ccccggcaga ctgcaaaaat tggttttctt
      421 tgtttcaatg cttccaagat cagtgcctgt tcaaattggt ctcgttgaat atttgtttcc
      481 gttagctttg gatgaggatc ttccatgtaa agttgtggac tgacatcttg accacctgca
      541 agaataagtt tgtcgatttg tccaatcagt tgaggagctg aatctggaga catgactggc
      601 agaataagag gcaatccttg agcatctaaa actgcatcca cgaatccttg cggtgtatag
      661 ctcacttgat ttccttggaa agtatctgtt gctcgaatca gttgatttcc ggcaatgccg
      721 atgattggtt tcatttgtat tccccctaaa gtagacttat tgtaacaagg atttaaaaaa
      781 aattaaacta tgtaagactt gcccctatac ttatctgttc ttgctgtgtt actatctgta
      841 aagagaaatt tcgtgtgaaa ttcatgacga tagttgaaaa tattgcttaa tttgttataa
      901 taaaaagtaa gcagaaataa aaaggttggg cgtaagcttc aaggatctag ttcttcaagg
      961 ggtgagaagg acattttatt gaaggggcct gcctatacta agttatcttt agaggtacat
     1021 actttcaatg aaaaaagaaa gcatgtacct cttttttaaa tcgaaaggaa gtgaagcaag
     1081 tgttagaagc tttggaacaa atccgaaaag cagaagaaga aaatgaaaag cgtgctcaaa
     1141 cactgcggca gcagctgaag atctatgcaa cagaaaaaga gcaggaattg gaaacgatcc
     1201 aaacaaacta tcgacaacaa ctaaaacagt tggtggaaga aaaagaacaa tttgaaatac
     1261 aagaattaga aaaagaaaaa atgaaattga aagcagaagc agacaaggtg caagcaactt
     1321 tgaaagatca atatcaaaag catcagagtc aagcgatcgg tgcgatcatt gaaagggtga
     1381 aagaaactta tggccgtcac taagatggaa aaagtgaccc tcatttcaga taaaagaaat
     1441 caggagagga ttttacaagc agtacaagga atccaaaacg ttgaaatccg ggatctgtat
     1501 caaaatagaa caaacaacca gtgggtggag aagtattttc cagataccac gattattgat
     1561 aaagaagcaa aattaagcgc tttaggtaat cggttgaaag acatccaaga agctatccaa
     1621 ttcatacaac accacggcga tagccgtcaa aagaaactcc atttgaaacg tcaagaactt
     1681 tccttacaag atttggaagc caattattcc gaagaaaatt tcataaataa attgaaagaa
     1741 gtccttgatt taaaagaaaa gtgggaacaa ctaactgaag aacgtgatcg tttagaagaa
     1801 gaggaagaat ggcttcttca ttggcaacag ctggatattg ctccaagtat gtatgcaagt
     1861 aagacgacca tttttgagat ggggactgtg agtgcagcaa attttgaaag tttcaaggaa
     1921 gaactgagta agcttgatga cgtttatatg gaagaaatag attatacgcc aaaacaagtt
     1981 tatctttcgt atgttgcttt aaaaaaatca gttgctgtta tagaagaaat tgctgggaaa
     2041 tacgggttca ataaagaaac gtatccttat gaacaaaatc ctaaagaaaa actgcaagag
     2101 accaagcagc aattatccat tgttcacgag cagcaaaaga agataacgac agcgttaggt
     2161 cagtgcagtg gctatatcca agaatttgaa tgggtagaag aagtcacact tgccgttgca
     2221 gaacgagaaa aaataaaaga acggtttatc catgcgactt atctgatcgt tttgcaaggt
     2281 tgggtagatg cagaagaaaa acagaatttg ctgcatgtgt taaacgaaac cgtatcagaa
     2341 gaagatatat acgtcagttt cgaaacacca aactcagaag agattcagca ggaagtccct
     2401 actaaattga aaaaccatcc gcttgtcgaa ccttttgaat tgctgactga aatgtacagt
     2461 ctgccaaaat atgaagaagt agatccaaca ccttggatga caccatttta tcttgtcttc
     2521 ttcgggatga tggtcgcaga tgttggttat ggtttgctga tgctcattgg gacaatacta
     2581 gctcaaaaac tacttgttct accacgcggg atgaaacgtt tcgtaaaatt ctttgagatt
     2641 ctctcgatac catcgatcat ctggggattg atctacagtt catttttcgg acagtcatta
     2701 ccaaaagaac tgtttggcat ccgcttgcct ttcccaatcc tatctacgac ggatgacgtc
     2761 aatacgattt tgattttatc cgtgattttc ggtctgatac agattttggt tggattattt
     2821 gtggcagcaa aggaaaatat caggagaaaa gattacttga gtgctgtcag tgatggattt
     2881 gcctggcaag ggatcctaat tgggatcgtt attgcgttgg ttggtgccat gttgataaaa
     2941 aatacagcat ttgtttacct tggcgctggg ctatcgatca ttgctgcctt atgtatcttg
     3001 atcgttccga ttatccaaac gccgtctaaa gtcaaaggtg ctgccaaagg tgcgtataat
     3061 ctttacggac tgaccggcta tatcggtgat ttagtcagtt atacacgttt gatggcattg
     3121 ggtatttcag gcggaagtat tggagcggca ttcaatatgc tagttgcttt catgccgcca
     3181 gctgctcgat tcagtgtagg gatccttttg atcatcgctt tacacgcact gaatatgttt
     3241 ttgacactgc taagtgccta tgtccatggc gcacgattgc agtatgtcga attttttggg
     3301 aagttttata caggaggcgg acgtgccttt gatccgctga aaacggcgga aaaatacgtc
     3361 aacatcaatc acaagaaaaa aaacaataaa taattggagg atttttatat aatgatggat
     3421 tacttaatta gtcaaaatgg tggaatggta tttgcggtat tagcgatggc aacagcgacg
     3481 attttttcag ggatcggttc agctaaaggt gttggaatga caggtgaggc agcagcagca
     3541 ttgacaacaa gtcaacccga aaaattcggg caggctctga ttctgcaatt gcttccaggt
     3601 acgcaaggtt tgtatggatt cgttattgcc tttttgattt ttatcaactt aggaaatgac
     3661 atgtcagttg tacaaggact gaactttcta ggtgcttctc tgccgattgc ttttacaggc
     3721 ttgttttcag gtattgccca aggaaaagtc gcagcggcag gtattcaaat tttagcgaaa
     3781 aaacctgaac atgctacaaa aggaatcatt tttgctgcga tggttgaaac atacgcgatt
     3841 ttaggtttcg ttatttcctt cctattagtg ttgaatgcct aagcataaag gaggaaaagc
     3901 agatgaacgc cattgaaaaa atcatctctc agatgaacga agcagcagag caggaaagag
     3961 cggcgttaga acaagaagag cgaatgaaga tcgatcaaaa ctttgaacaa aaacgtacgc
     4021 aagttgaaac agaacatcaa aaacaaaaag aaaaacaaat cgaattactc gaaaaaaaat
     4081 accgtcagct aaggaatcgc cagcaagtag aagtacgaca agaaaatttg aatgcgaagc
     4141 aggaattttt aagacgattg tttgcagatg cggtaactga aatggaaaat tgggatgaat
     4201 ctgaacaaat ccagtttatc aaaaatgcat tgtattcatt acctttgaca ggaaaagtag
     4261 cattcattgc tggagaaaaa tcagcagcct atctttctca gacgttgttg gatgaatgga
     4321 acaatgaact tccctttatg atggttttaa gtgatgaaac tgttgctgat caagcgggtt
     4381 ttctgataaa cgatcaagga gttcagtata attttctttt cagcagcctt gtacaagata
     4441 tccaaggaac gatgagtttt gagattgcca atcaattatt cgaataagga gggatcagtg
     4501 tatggaatac cacgaactaa atcccttgat tcgcggaaga gagcttgaac ttttgtccaa
     4561 tgagacattt gaacggatga ttcaaacaaa ttctgtcgaa gcacttggtg aaatattgaa
     4621 atccacgatt tacagtccat atatttatga cggatatgaa tatgacttcg aacagaatct
     4681 tttaaaggag cagagccgtc tatttcgctg gttaaaggaa tcagcaccgg agcctgaaat
     4741 cgtttggatc tatacgatgc gttatacttt ccataatctg aaggttttga caaaagctga
     4801 gatgacaaat caaaatctgg accatttgta tatcgacgat ggattttatt cattagaaac
     4861 cttgaaggat gccattcata cgcaagtttc aaatgaactt cctgaaagca ttatggacag
     4921 tatacgagaa gtacgtgatt attttgaaga gtctaccatt cttcagggaa ttgacgtgat
     4981 ttatgatcga cagtttttga cagagcagcg acggcttgcc gataagctag gatatcaaga
     5041 attgctgaca gaagtcattg cttttattga cttgaccaat atcacaacga tggctcgagg
     5101 aatattgcag aaaagaacat atgggttcat gacgacggta ctttccagct cgggaagtat
     5161 cgaaaaagaa gtatttttga actttgtaga taaagatatg gaagattaca cgaattttct
     5221 attagagaca gattatgctg attttctgcg ccctgtcatc cgtgagggaa atattgatct
     5281 cgtaaagctt gaacagttga aagatgatta tctaagttca ttgtatcaag aagcacaaac
     5341 ccaggctttc ggaccactgc cactactagc ctttttgaac gcaaaagagg tagaaagcaa
     5401 aaaccttcgt ttactggtta tcggcaaaag aagccatttc gaatcagaaa caatcagaaa
     5461 gcgggtgaga caagtctatg acgcataaga tcggtgtagt tggcgacaaa gattctgttt
     5521 tgccattcaa attgtttggc tttgatgttc gttatggtac aaccaagcaa accgttcgtc
     5581 aggcaattga agaaatggca aagaatgagt atggcgtgat ttatgtcaca gagcaatgtg
     5641 cagcattgag cccagaaacg atcgaacgtt acaaggaaca aatgatacct gctatcgtat
     5701 tgattcccaa tcatcaagga tcattaggga tagggctgga agaaattcaa aaaaacgtag
     5761 aaaaagcagt aggtcaaaat atcttatagt atgaaaagga gatagaaatt ttgcaaattg
     5821 gaaaaatcat aaaagtctcc ggacctcttg taatggctga gaacatgtct gatgccagta
     5881 tccaagacat gtgtctagtg ggagatctag gagttatcgg agaaattatc gagatgcgtg
     5941 gcgatgtggc gtctattcaa gtatatgaag agacttctgg aattggtcca ggcgaaccgg
     6001 ttcgctctac tggtgaagct ttatctgttg aattaggacc agggatcatt tcacaaatgt
     6061 ttgacggaat ccaacggcca ttagatacct ttatggaaat cacacaaagt aatttcttag
     6121 ggcgcggagt tcaattgccc gcattggacc atgagaaaaa atggtggttc gaaccaacag
     6181 tagaagccgg agaaacggtg agtgctggag atgttatcgg gatcgtagaa gaaacaaaag
     6241 ttataaaaca taaaatcatg gtgccaaatg ggataaaagg aacgatcaaa acaatcgaaa
     6301 aaggttcatt cactattgat gaaacagtat gtgtcattga aaccgaaaat ggtgacaaaa
     6361 atttgacgat gcttcaaaaa tggccagtaa gacgtagtcg gccaatcaaa gaaaaattaa
     6421 acccggatgc acccatgatc actggacagc gagtgattga tacattcttc ccagtgacaa
     6481 aaggaggagc agcagctgta cctggtcctt ttggtgcagg gaaaacagtc gttcagcatc
     6541 aaattgcaaa atgggccgat gtggacatgg ttgtctatgt gggatgtggc gagcgtggaa
     6601 atgagatgac ggatgtttta aatgaattcc ctgaattgat tgaccctaat acaggagaat
     6661 cgttgatgga acgtacggta ttgattgcta atacatcaaa catgccagta gcagctcgag
     6721 aagcttctat ctatacggga atcacaattg cggaatattt ccgtgacatg ggttatgacg
     6781 ttgctatcat ggcagactcc acttcacgat gggcagaagc acttcgtgag atgagcggac
     6841 gtttggaaga aatgcctggt gatgaaggtt atccagctta tcttggttca cggcttgcgg
     6901 aatactatga acgatctgga agagtcattg cattaggatc agaaggtcgc gaaggaagta
     6961 taaccgcaat cagtgctgta tccccgtctg gaggggatat ttcggaacca gtcacacaaa
     7021 atacattgcg ggtcgtaaaa gtattctggg gtcttgattc cagtttagcc caaaaaagac
     7081 attttccttc gatcgattgg atacaaagct attctcttta ttcaacagaa gtagggaagt
     7141 atatggatca tatccttcaa gctgactggg catccatggt caccgagggc atgcgtattt
     7201 tgcaggaaga acagcagctg aatgaaatcg tgcgtttggt agggattgat tccctttcag
     7261 ataatgaccg tctgacatta gaagtggcaa aatccattcg tgaagattat ttgcagcaaa
     7321 atgcttttga tgatgtcgat acattcactt caagagagaa acaattcaaa atgttgaaga
     7381 tgatcttgac ctttggtgaa gaaggacgcc gtgcgttgtc gttaggtgct tatttaagtg
     7441 agatcatgga cggtaccgta tcgattcgtg aaaggatcgg acgaagcaaa tatatcccgg
     7501 aaacagaatt agaaaaacta gatcaaatta ttggtgaaat caagcaaacg atgcaaacga
     7561 tcgtttcaga aggaggcatg agcaatgatt aaagaatatc gtacgatcgg tgaagtcgtc
     7621 ggtccattga tggcggttga aaaagttgct ggtgtcaaat atgaagaatt aatcgaagtc
     7681 cgtatgcaaa atggcgagat tcgtcgtgga caagtattgg aagtccaaga agataaagca
     7741 atggtccaaa tattcgaagg aacaagtggt atcaatttac gtgactcttc tgtacgattt
     7801 ttaggccatc ctttagaact aggtgtatcg gaagacatga tcggtcgagt attcgatggt
     7861 ttgggacgac cgaaagataa cggaccagag atcctgcctg aaaagcattt ggatattaac
     7921 ggagaagtaa tcaatccagt agctcgggat tatccagatg agtttatcca aacaggtatc
     7981 tctgcaatcg atcatttgaa tacattagtt cgtgggcaaa aattaccagt attttctggc
     8041 tctggtcttc ctcataaaga attagctgct cagatcgcgc gacaagcaac ggtactcgat
     8101 tcatcagatg atttcgccgt agtctttgct gctattggaa tcacttttga agaagctgaa
     8161 tttttcatgg aagattttcg tcaaacgggc gcaatcgacc gttctgtcat gtttatgaat
     8221 ttagcaaacg accctgcaat cgaacgtatc gctactcctc gtatggcttt gactgctgca
     8281 gaatatcttg cttatgaaaa gggaatgcat gttttggtta tcatgacaga catgaccaac
     8341 tatgcagagg cattgcgaga gatttctgca gcacggcgag aagttccagg aagaagagga
     8401 tatcctgggt atctttacac gaacttagct actttattcg aacgagcagg aagaatccgc
     8461 ggactaaaag gatcggttac tcaaattccg attctaacaa tgccagaaga cgataaaact
     8521 catccaatcc ctgacttgac tggttatatt acagaaggac agatcatctt gactagagag
     8581 ctttacaaga gtgggattca gccaccaatt gatgtactcc cgtctctttc gcggttaaaa
     8641 gataagggaa ctggacccgg aaagacaaga gaagaccatg cagctacgat gaatcagctt
     8701 tttgcagcct atgcacaagg aaaacaggca aaagaattag ccgtagtttt aggtgaatct
     8761 gctttatctg atgtagacaa gatctatgcg aagtttgctg agcggtttga aaaagaatat
     8821 gtcaatcaag gattctacac gaaccgttcg attactgaaa cacttgatct agggtgggaa
     8881 ctgctatcga tgttgccaag aaccgaatta aaacgaatca aagacgacat gctggacaaa
     8941 tatctgccag aagggaagtg agttctgatg gcacgattga atgtgaatcc gacccgcatg
     9001 gaattgactc gattgaaaaa acagttgaca actgccacaa gagggcataa attattaaaa
     9061 gataagcaag atgaactgat gcgtcaattc atcctgttga ttcgaaagaa caaccagctg
     9121 cgtcaagaaa tggaagagtc tatgacagaa gcaatgtctg attttgtatt ggcaaatgct
     9181 tcgatcacgg aacctttcat cgaagaatta tttgttttgc cggcggaaaa cgttgatttg
     9241 tctatcgtag acaaaaatat catgagtgta aaagtacctg tgatgaattt tgaatatgat
     9301 gaacagctga cagacacacc tttggaatac gggtatttga attccagtgc cgaactggat
     9361 cggtcaatcg atcggtttac taccctgctt ccaaaacttt tggaactgac agaaatcgaa
     9421 aaaacttgtc aactgatggc gagtgaaatc gaaaagacac gtcggcgagt caatgcatta
     9481 gaatatatga cgatccctca attagaagaa acgatctatt atattcgtat gaagctggaa
     9541 gaaaacgagc gcgcagaagt aacaaggttg atcaaagtaa aaaatatggg taccactgac
     9601 taagtggtat ctttttttaa aagaggaaaa cgatgattaa acaaagcaaa caaataaaga
     9661 aacgtttgcc ggctgttcag cttctcgcat taggattttt tctcctgatt ttagttggcg
     9721 gcggcctact taccctacca ttttttagta atagtgggca tgggacaaat tttatcgatg
     9781 cgcttttcac tgcaacttct gctgtctgtg taacgggatt gacaacattg aatacagcag
     9841 agcattggaa cagtgctggt caatttttga tcatgctttt gattgagatc ggcggtcttg
     9901 gttttatgat ggttccgatt ctatgctttg cattgataaa gaagaaagtc agtctcagca
     9961 cacgtattat attaaaagaa gcgctgaatc tagaagaagt atcaggtgtg atgaagctca
    10021 tgctttacat cctaaaattg gcctttgtga tccaattgat tggtacggtt gctttatctt
    10081 ttgtatttgt gcctgaattt ggctggttga aaggtatctg gtacagtttg ttccatgcaa
    10141 tctctagttt ttgtaatgca gggtttgatt tgttaggaga cagtttagcc aatcaccagc
    10201 aaaatgttta tttgatcatg gtggtatctg ctttgatcat ttctggtggg ttaggcttca
    10261 tcgtatggcg tgatttactg gaatatcatc agaaaaagaa gatgacgatt cattcaaaaa
    10321 tcgcattaac cgttacaggt acccttttat taggcggatt cgttgtgttc tttttcacag
    10381 agcgcaatgg ggcaacgcta gtggaaggga atatgtttca acgattggca aacacctttt
    10441 ttatgagtgt gacacctaga acggctggat attattcagt tgattacctc catatgagcc
    10501 atgcaggtct gattttaacg atgtttttga tgtacatcgg cggaacatct gggtcgacgg
    10561 ccggcggatt gaaaacaact acgttaggtg tgttgctgat ccagatgcgt tcgatgttga
    10621 aaggacgtac gagagcggaa gcatttggcc gtaccatccg acaaggcgca gtgttgcggg
    10681 cacttacttt attttttgtt acgttgacgt tgtgtgtagt cgccatcatg attttatcgg
    10741 tgactgaaac gatacctaaa acttctggca tcgaatatat tgcttttgag gtcttctctg
    10801 cgtttggtac agtaggtctt acgatgggat tgacgccaga cttgactgca atagggaaat
    10861 tgatcattat agcgcttatg tacattggac gagttggtgt attgactgtg gtcttctctc
    10921 tgatgacaaa agcacaaaga acacaatcaa aatacaaata tccagaagaa agtgtcatga
    10981 ttggataatt tcatcttctg acagaaacct agtgatttag taaaataaga aaataaaata
    11041 atttttcttg cttcttggat actttaagtg caaaatgttc gtttattttt tgaggcacta
    11101 cccgttgttg aacctcgggt agtgcctctt ttttattgaa aggaatcaat aagatttgat
    11161 tcctttattt atcaataggt tataattcat atttttttat tttttctttt acgatactta
    11221 gtccagaagc taaaatttca aggttattag aggataggga aattcgaacg aatgcatctg
    11281 gttgcgtacg tgtagtaaag cgtctggaat gataaagtcg gataccgtta gcaagaaagt
    11341 cattctcaat tatcttaggg tcaatctcag gatcaactgg aagaattcga tagtaaggga
    11401 cacgattcgt tggtttttct actttgaaaa gagaatcaaa aagctgattg gctctttttg
    11461 taagacttaa tttagtttct aagatcctat ttgcttcatt tgaatggagg atttgagtga
    11521 tgacttccac atctaatcca gaagttttca catttatatt aaaaattgct tgttctatct
    11581 tgctttggac atgtactgga actacaagat aagcaactcg caatccagaa caaataaatt
    11641 ttgtcattcc agctaagtag attgtttggt taggtaataa attttggaaa ggaggaatga
    11701 tcgtatgttg atagtaagtt gttaaaaatg aatggatatc atcttcaata acccatagac
    11761 cttctttttc aatcacttcg gctaattgct ctcgtctttt gtgagagatt tgaaacccta
    11821 taggattatt acaagaaggc ataagaaaga caccatgaat cttctttttt ctacattcct
    11881 gaatcaatat atcaggtcgc attccttgct cgtcaaaatc aattggaacg atctctaaat
    11941 gatataactg agccaattca atgaaattag cgtatgtata tcggtctgtt gctattctat
    12001 cacctggaga gaaaagtgca gaaagtgtga tagccaatcc attttgaaca ccggaaacaa
    12061 tagatagaga ttctaaggac ggaatgactc cttgagtttt taaccaattt gctgcagtag
    12121 tcaattgaaa atttgtgcct ttgggatctt ggtaattaag caaaccaaca agatttgggt
    12181 tattactagt tgattgaata aacgggacaa cgaatttatt acattcttca aaagaactaa
    12241 ccagaccaaa atcaatgata tgttcggtta cttcattggt agagatggtg atggactcaa
    12301 agacattggg agaaataaat gtgccacttc caatattcgt atacagcaaa cctttctcaa
    12361 ttccatattt ataagccttg ccaacggtag taaaatttaa atctaaataa tcagctagct
    12421 ctctttggga gggtaactga gtgtttttct gtaatttatt ctttaaaata tcttgttcta
    12481 attgttgaat caacgatcga taataaggac gcttcaattt ttgtttgtct ggtcgccaag
    12541 ttaacgggta attgtcataa gaattaatag gcattttctc cactccttaa aattgtatac
    12601 catacaattt taccattgat tgtattgatt ttagaagtta atcttttgtg aggagggaat
    12661 tgtctatgaa aattattaat tttgaggaag tagtagcttc ttttagtttt gaagaagcga
    12721 ttcaagtaat gaaaaaatgt ttttttgatt atgaaagtgg taaaatcagc caaaatcctc
    12781 gattggtgga aattttacct gacggaaaag gcaaaaatat gtttgcgatg atgcctgctt
    12841 atttggggga gaacagatat tttggcacaa aagtcattac cgcttttccg gataatcatc
    12901 agtatcattt accttctcat ttaggagaga ttttcttgtt tgatgctcaa aacggactac
    12961 ccaaagcatt gataaacgca aatgcagtca cctggattcg aacagctgct gtatctgcgc
    13021 tggcaacaga ttacttagca aatccgcatg cagaaacact tgctttaatt ggagctggtc
    13081 aacaagcatt gtcgcatttg actgcgattt ctaccattcg tcccatcact acagtgtttg
    13141 tttacgatat tttagaagaa agacgaaata cttttatcgc taaagctaaa aaacagtatc
    13201 ctaaaataac gtttataaat gcaatatcag tagaagatgc agtacgtcca gcggaaatca
    13261 tttgtacgtt gacctctagt aaagaggcat ttcttcaaaa agagtgggta agggaagacg
    13321 cacatatcaa tgctgtgggc acatttactc ctgatacaag agagataagt acatcgttga
    13381 tggaaacttc tcaaatctat gtagatgatt actcagctgc cttaacagaa agcggggacc
    13441 tgcttatagc catggcagaa ggagatttag aggaatcttg tattgttggt acattaggcg
    13501 aattagtttc tgagaaaaaa acattttata aaggagaatc gaggccgacg attttcgacg
    13561 cagttggtct agctattgaa gatctttgtt gtgcagaata tatttataac aaattagaga
    13621 aaggggcttt aaaatgaact tagattatta cgtagttgat gcatttgcag atgaggtgtt
    13681 caaaggaaat ccagcagcgg tttatgtgct agaagaatgg ctgccagaag gtacgatgca
    13741 aaaaattgcc attgaaaaca atttatctga aacagcattt acagtgaaga aaaaccaaga
    13801 atttgagctt agatggttta cgccagatcg agaaatcgat ctttgtggtc acgcgacatt
    13861 agcaacagca tttgttttat ttaactatta caaaatacct gatgaaacga ttaaattttc
    13921 tactcaaagc ggtaatctat ttgtgacaag aattaaagat tattattaca tggattttcc
    13981 tagtattatg ccgaaaaaag tgccgatatt gtctgaatat gaagaagcga ttggggctaa
    14041 gattaaagaa gcctacttag cacgtgattt atttttcgta ttagaagatg aaaaaacggt
    14101 ggctaaattg cagccagatt ttacagctat caaaaatttt gaattaggaa taggagtcat
    14161 cgtgacagca gaaagtgtgc aaaaagactt tgtttctaga accttctttc caaaattaac
    14221 aatcaatgaa gatccagtat gtggatcagc acattctaat cttattcctt attgggcaga
    14281 aagaatgaat aaaacaaaat tgtcagctta tcaaatgtca tcaaggggcg gggatctaga
    14341 atgtgaatta aaagaagatc gtgtgataat aggaggaaaa gcagttttgt tttctaaagg
    14401 tacctcgttt atttgatttc tttcaaatga attaaaaata aattggtgga gagtggcaac
    14461 cttatagcta caatggttgt taaaaaaaga cgcaaacttt ttcaatttaa tgaaaagttt
    14521 gcgtctcatt ttaattattg gtgtttgttt tttattcaat caagagtaaa atgccccata
    14581 ctaactatcc tcggatgact tcgattttat aaccatcagg gtcaatgaca aagtagtaag
    14641 aaggcgcagt gccaggtaat ccttttaagt cagtaatctc aagaccagca gctttatgtt
    14701 gctcgtgtaa tttttctaga tcatcagctg ctatagcgat atgaccatag ccgtctccta
    14761 gatcatacgc accatgatca tagttgtaag tcaactctaa ttcatagtca tccccaggaa
    14821 gtgtcaaata aacaagtgta aatttgtttt ctggaaaatc acggcggcgg ctttcttcaa
    14881 aaccaaatgc cttttgataa aattcgattg atgcttcaag atctttcact cgaacacatg
    14941 tgtgagccat tttcatagat tcatctctcc ttttcctttt actcaatcat aacaaaaaga
    15001 agtaaggaaa tagttgattt gtgcttgttc atgagaaatt tctcatgtaa aatggataga
    15061 aaataaaact gttaaggtgg aaactattca tggataaacc aatttttggt gaaaaaaagc
    15121 aatttgctga ttatcaaacc cgttatgcag catatattgt catagaaaga gatcagcaga
    15181 ttgcagtaat cgaggctcca aatggtgctt tttttcttcc tggcggggaa attgaaggag
    15241 aagagacgaa agaagaagcg attgtccgtg agctgataga agaaatggga attgtggcag
    15301 aaatttcttc ttatctggga gaagctgatg agtattttta ctcgaattat cgtgcaactt
    15361 attattataa tccaggatat ttctttgttg ctgattcttg gaagcgtata ggagaaccaa
    15421 ctgaaaagac caataaaatt tggtgggtga gccctcaaga agcgcttgtg aaattgaaac
    15481 gtggtagcca ccgatgggca gtcgagagat ggatcgaaaa aaataaaaac tgaaagaaag
    15541 cgagtagatc agctttctct cagttttttt atttattaca gtacatattt tttgattgct
    15601 tcagctacac cgtgctcgtc gtttgatgaa gtgatgacat ttgctgcttc tttgacaaga
    15661 gaaacggcat ttcccattgc tactcctaga ccagcatatt caatcatgga taagtcgttc
    15721 tcgttatctc cgatagccat gatttcatct tgatcaatac caaggtgctg tgcgagattc
    15781 gcaactgccg cacctttact tgcttcttta tttagtactt cgaagtaata gggcgcactt
    15841 ttgaccgttg tgtatttatc acggaaagtt tgcggtaaac gcgcaattgc tgcatctaaa
    15901 atttctggtt cgtcgatcat catcattttg acgatgttca tgtctggtgt catttcttca
    15961 ggtgttcgat attttagcgg catcttgact aaacttgctt cgtggatcgt ataagcgctg
    16021 atatctcgat taggcgtgta gatcgctttg tcatcgatcg aatggagatg actgccgaca
    16081 cggcgtgcca tcacttcgat ctctaggaaa tcttcatgtg tcagtccgtg acgggcgatg
    16141 attttacctg atttcgtttg ttgtacgagt gccccgttat aagtaataac gtaatcatta
    16201 tcttgaaaaa ggttcaactc gtttaactgt tcctggacac ctggtaaggg acggccagtg
    16261 cataaaacga tcgaaacacc ttttgcaaca gcagcggcaa tcgcttcttt tacttctttt
    16321 gttacttctc ttttctcatt taataaagta ccatcgatgt cgatagcgac tagtttgata
    16381 gacataattt cctccaatat attttaattt atttcaatca atgcgccatt acggatatgg
    16441 ctggcaaatt cttgataagt ttcgtcaaat aaatcataat gatcacgcaa ggacggatca
    16501 accatttctt ttggaaagaa aaatcgctca tctccttgtt cttgtcccgc taatgcagca
    16561 acaagctgac taacctgtga taattctatc aaagttccgt catttcgcaa aatctcgatt
    16621 tgtgtgcgat gtctgccttg ttctgggcga taaaaatcat aaggtagatc gtagctagaa
    16681 ttcacagcag tgtaataaac aggattatat ccgacctttt ctactaacaa agtgagttct
    16741 tggactaatt ttgaatcccg gttgccagaa aaagtagcag atttcagtgg tttacgattc
    16801 aaaaaacgtt tagctaaatc atttaaaata ggatcagcag attcagacca ttgagtaaag
    16861 taagtgccca agaccccatc atcaagtttg aggtattctt ctaaagtaaa gcttccgtca
    16921 agaaatggca gaagtaattg agaatggagt tcaaaactgc tggcatcttc ttgatacaac
    16981 tcgtgtgcgc gatgaagcag atgctccaaa attacttcca taccacggga aacaggatgg
    17041 aagtacactt gcacatacat ttgatatcgg ctaacgatat aatcttccac tgcatgcatg
    17101 ccactcatgg aaaaagcgat cccgccttca taaggacgaa tcacacgtaa tattcgggta
    17161 agatcaaatg ttccatattc tgttccagtg aaataagcat cccgaagcaa atagtccatt
    17221 cgatctgcat cgatctgact agaaatcatt tggacaactt gtgggttggg gtaagttttt
    17281 tggatcacac tagctacctt ttcaggaaaa ccttcttcta ctcggttaag aatctgatac
    17341 acttctgttt ctggagaagt gatgatctct acagtgatgg cttcatgatt cgtatggaag
    17401 atatgttcaa atgtatgaga gtaaggacca tgaccgacat catgcaacag tgccgcacat
    17461 aaagtcacca gccgttcctg atcatcccag cctccgttgc ctattttttc ttttgaaaaa
    17521 tttcgagaaa agatatcgca gattcgtctt gaaatctcat aaacgcctaa tgaatgagta
    17581 aaacggctat gttctgctcc gtgaaaagtg aaagaagaag tgcctaattg tttaatgcgt
    17641 cgtagacgtt ggacttcttt cgaattgatc aaatctaaga ttacttgatg ctggacatgg
    17701 atatagttat ggactggatc acgaaaaact ttttccatag gcagcatttg atatttataa
    17761 ggggttgtca taatgtagct cctttatgtt attacgttgt tccattctct ggatttggtt
    17821 gttccattgc tccagctgga tttctttttt tagccagtcg gtagaatcct gtaaatcact
    17881 tgcagaatac agcttttcaa aagcttttgc aaatgcttct ttgacttctt ttacaaaaag
    17941 gggttggtca agcagttctt caagtgttgc cattgtagca ggatccactg caggatagcc
    18001 atttgtacca aagtcttctt ttaaagcaga ttgataaaat cgcttcatga cttgcccgcg
    18061 tttttcctga ttaccgttga cacttaagta catcataaca gaaatcccat ttttgatccg
    18121 acgctgagcg ataccggcaa attttttgcc atggatgctt aaatcataag ttccaggaca
    18181 ataagaatgg gtgatttcga atgcttgtat cggtgccttt ggaaaagcgt atttagtcaa
    18241 taggagcatt tgctcgtaag cgtcatctat ggttattttt tttgtgtcac tttgggggaa
    18301 aatcaaagaa atattcaaaa ttccttcatc tgatacgatc cctaatccac ctgaatttct
    18361 gatcactgga gcgtaacctg catctttcag tgtttggatc ccgtcactta gaaaaggagt
    18421 tcgaacatcc ttcataccta agatgactgt gcagtcatat tgccagaaat gtaaaaagat
    18481 ttgctggtgt tctttagaat attccgttaa tatatctgtt aatgcaaaag gggaaaagtc
    18541 gtttttttga taaatgtgct gatctagtcg aatcgcaggg ttcattcaac cggctccttt
    18601 gtataaattc atattttcta caatattata ctctaaaatc aaagagttgt tttggaaaaa
    18661 aggttttctg taaaaatgga caagttggtt gacaaaacaa aagaaactag gaacaatgtg
    18721 gctatgagaa gtaaaggaga atgaataatg gaattatcac aaggaacacc gatttcaatc
    18781 aaattgcgta caaaagtaaa acagcaaggg gaagtgcaag atttttattt tgatttgaaa
    18841 gggcaaatgg tgacaattgg cgatacgctg tacatacgtt ataaagagat tcaggaggag
    18901 acgggcgatg agattcctgt aacgatcaaa ttagttccag atggtcatgt acagctgatc
    18961 cgtgcaggag aaatgcggat gcgtttgaaa tttggctata aagaacggtt agaaacatcc
    19021 tatcgaacac catacggtat gatccagatt gctactttta cgaaagaact gcatgttagt
    19081 ttgaaagacc gtcccacagc agggaaggtt cgaatcgatt atgatctgtt catgggaccg
    19141 gaaaaaatcg gcgaatatta tttgaccttg gattttacag cgtaaatata aaagaaaagt
    19201 ttgatttttt gtgtctgatt ttgtatgata aggagtagac tgtgaaagga cgtgtcctga
    19261 ttggaaatta gtgtatttga aggtgcaaac aaaagcgaat tatcaatgat cgaagtagca
    19321 cacgcgatct tagaacaacg tggcgatgtc atggattttt ctgatttagc taaccagatc
    19381 caaaactatc ttgggaaatc agacagtgaa attcgtgatt cactcgcaca attttatact
    19441 gacttgaata ttgacggcag ctttatctca ttaggggata atcgttgggg cttgcgttca
    19501 tggtatgcta tcgattcgat cgatgaagaa gtaaaccatg gaatggacga agaggatgaa
    19561 gatactccac gtcgccgtaa acgcaagaaa gtcaacgcct ttatcaatga tgatgaagac
    19621 gcgatcgatt acaatgacga tgacccagaa gatacagatc tgacagaaga agatgacgat
    19681 gatctgtttg atgacgatga tgatgaagat gaagaaatcg ctgcttataa ttctgacttg
    19741 caagaaatcg gagcagatga cccgacagat gatgaagaag acttgccagg aaatatcgaa
    19801 gaagatttaa gtatcataga cgaagacgat gatgatgaag attatgaaga ggaagaattt
    19861 tctgactctg aaaaagaata aacattattt tcttacgaga ggttgtgaca gagacgatca
    19921 gcctatcaga ataaggaggt gtgacacgat ttttgtcaca ccttcttttt tgtatataaa
    19981 aatcccaagt aaatacataa aaaataaaaa aattgaaact tttagcgcac cttgtacgtc
    20041 atattaagtg aggcagaaag gagtggagaa tgatttttac tggatataag aaaaaacggc
    20101 agatcgaaaa ataccgtcac ctgctgaatg gactcatcga aactgattat caaaaaatgt
    20161 tccgtatcac gatgaattac gtacatgata aagaagaggc cttagatgtg atgcaagata
    20221 gttttcataa agccctgaac tattttgaaa aagaaaaaga gatcgagcat tttagtgcat
    20281 ggttttatcg gatattgatc cgcacggcat tagatagttg gcgcagacaa aaaaggacgc
    20341 ctgtggactt atttgaaatt caagatatcc cccaacagtt acctttagaa acttccgttg
    20401 cagagctcca tatgatttta gataaaatag acagtccgga aaaagagatt ctgatcctct
    20461 acttttttga aggatttcgt ttgaaagaaa ttgcgactat cttggatatg aatgaaaaca
    20521 cagttaaaac gaaaatgtac cgctcattga atgctttacg tcaaatccta gaatgaagga
    20581 gagaagagtg ccatgtattc ccaaaaagaa aatttaaaaa aactaaagaa aacttataaa
    20641 gaacaagcaa ttcccaaagg gcttaaagag gagatccgcc agcgttttat tatcgaagag
    20701 caatcattta taaaagcaaa gcggagaaag agacgtctcc aagggttcgg agtatctttg
    20761 gctatcgtaa tgattacaac aagtagcctt ctttttaata gtcaagttcg aagctttgca
    20821 gaagatttac ccatcttagg atcagtcatt gaattgatct taggcgaacg ctttactgat
    20881 cgatctgaaa aaatcgatat tcaggtaccg aagatcaata cacaaaatga gaaagaaaat
    20941 aaaacaattc atggattaaa tcaaaaatac tttcgcgaag gacaagcaga ttttgaaaaa
    21001 gctgaaaaag aatatggaaa ttttgaaacg gatcattacc aagttttggg agattatcag
    21061 aaaatcgtag acgataaccg gtttctcgtg attgaaaggc agatcaccca aacagcagca
    21121 gatagccatg ttgaaaaaag atacgacaca atcgacaaga aaaacagtgt ccagctctca
    21181 cttccacttt tattcaaaga tgacacctat ctatctgttt tgacaaaaga agtcaaacgg
    21241 cagatggccg aacaagtcaa ggaagatcca tctaagtact attggactga gcaagatatt
    21301 caagaaggga cgatagaaaa accaacactt gttacgccta cacgaagttt ttatttgaac
    21361 aaagagcatc aattagtttt gactttttct caatacgaaa ttgctcctgg ttatatgggt
    21421 acaccagaat ttgtcattcc taagtccgtg acaaaaacga ttttggcatc ggaagattat
    21481 ttggatcatt agacgagaag tagttgcgac tagcagtttc aattttcaag taagtacaag
    21541 taaataataa gaagagaaaa agagaaagaa gagatatagt gaaaagaaat tatttagtta
    21601 tcagtattat tgctttatcc gtgcttttgt ttttcgaaat tacttcggtg gcctccttga
    21661 tcaccgaagt aaaaggacaa acacactctt cggagcattc gtcacaatct ttcaaacaat
    21721 cagaacaatc aaccattaaa agccaatcaa ctgaaacagc taacgtcaaa gaagccattc
    21781 aagaatcagc ggaaaaggta caaagtcagt catcacaatc aacacaagac agtacaaaaa
    21841 cgagccgaga tccggctgtc aaaagcgtcg caatcagctt tgatgatgga ccaggagcga
    21901 ccacaacacc gcagcttctg cgaatcttaa aagaaaaaaa cgtgcatgcc acttttttcg
    21961 tcttaggaga aaatacggcg cagcatcctg aaatcgtgaa gcagacagct gaagcagggc
    22021 acgagattgg caatcacaca tacgatcatc agaatctggc cactctttct gcccagtcga
    22081 tgacggagga agtgacgaaa gcagacactg agatcaaaaa agtcactggc aaaaccccca
    22141 cttttgttcg tccgccctat ggttctgtca cgaatgtagg agcaacgatc atccagcgac
    22201 cgatcatcga atggtctgtt gattcagaag attggaagac aagaaatcct gatttgattt
    22261 tgcagaagat ccaagcaaca gtatacgatg gggcaattat tttattccat gatatctatc
    22321 ctgaaacgat tcgtgccgtt cctcaagtga tcgattatct gaaagagcaa gggtatcgca
    22381 tcacgacagt tggggattta ttagggcatc caacggctgt cgagaactat tatggcagaa
    22441 acgatcatcg gccagttcag taagtgagac aagtatcttt aataaataac acattttaat
    22501 aagtagttga acacataact accttacttg caaaggctta acaatatcta tacaggtaca
    22561 acaaaaaact gtccaccaat tttctttggc ggacagtttt cacttaaaaa taaagaagca
    22621 actattcgta aattatgcta aaatagcagt agaacgcgtc ctgagggaat cgaacccccg
    22681 tctcaagaac cggaatctta cgtgatatcc actacactaa ggacgcaagc actgggataa
    22741 ttgtaactaa acagataaaa aattacaagg ggattggaca aaaaaatgaa atttgagcaa
    22801 aatttttcac aaaaacagca gcagacacag aaactagcca tgacgcaaca gttgcaacag
    22861 tccatccaag ttttacaata taacagtgag gagctgttgg cattcgttga gaatcaagca
    22921 atggaaaatc cattggtgga agttgttgag ccggaatggc agccagatta cataaaagct
    22981 tcttcatctt cttatgaagg agaagagaca aattacctaa atcagattcc tgatacaaaa
    23041 gggtctttat ttgattcatt gatcgaacaa gtccatttga attaccgaga tacttttttg
    23101 aggaaaatcg ttctttatct tgtagaatac atcgatttga acggattttt gacgattagt
    23161 ttagaagaag ccggaaaaga gattggagct acgcctatcc aaatgctcga tgcgttgact
    23221 ctgattcagc aattagaacc agctggagta ggtgcacgta gtttgcaaga atgtctcatg
    23281 cttcaaacag aacgagatga ttatgcgccg gaattagctt atatcgtgtt ggaagaatgt
    23341 tttgaggaac tggtggagcg aaaatggaaa gaaatcgcac aacgtttttc tgttgatcta
    23401 catgctgttc agcagatatt cgactattta caaacgttga gtccaagccc tggccggatt
    23461 ttcgaccgta gcagcgaatt gtacattatt cctgatgtcc gggtattagt ggacgacgat
    23521 aaaaatgtac aagttatctc aaaccgaaaa aatcagccaa atatacgctt tcaagagagt
    23581 tacttcaaac aaatgtcaca acaagcagat aaagaaactg aaaattattt aaaagaacgg
    23641 aaacaggaat tcgaatggtt gaagaaaacg atcctacaac gtggggatac aattttgcgg
    23701 gttgctcaag tcattgtttc ccggcaaaaa gctttcttta tcgacaagga acgtccaatc
    23761 aagcctctta cgttaaagga agttgcaact gagatagatg tgcatgaatc cacggtcagt
    23821 cgtgcagtca acgggaaata tttggaaaca gacttcggtg tatttgaatt aaaaaaattt
    23881 ttcactacac gaattcctac gaatagtaca gaacagacag aagacctttc agcagataca
    23941 gcgaagaaaa agctgcagga attagtcgat caagaagaca aaaacaagcc gttgtctgac
    24001 cagaaattgg ttgaattgct gaaaaaagat gagatagcga tctcaagacg aacagtagca
    24061 aaatatcgtg atttattagg aatcccgagt tcatcaaaaa gaaaacgtta tgataactaa
    24121 tcagtcaaaa gactagaaaa agtatttcac tttttctagt ctttttagtt gaaaaacgag
    24181 agcaaaactg ttagacttac agtcgtgagg ttgagatttc cctttattga tgtgaaattc
    24241 gaatagttaa tttctttttt tatcgtatat gggtcgtttt aagtctacat tggacgtaaa
    24301 gtgtccaact aaggagatcg ctatgctgga tgaaataaaa atgattgaat ctgttgcacc
    24361 agatattatc gaagtgttgc aagaacgcta taaaatttta cgaaatatct attggatgca
    24421 gccgattgga cgtcgaagtt tatcggaaag tatggggatc acagaacgag tattgcgaac
    24481 agaaacagat cttttgaaaa atctgaattt gatcgacacc tcaaaaagcg gtatgacgct
    24541 gacaactaag ggcgaagaag tttatcaaag ccttgaaaac ttcatggatc agcttttagg
    24601 tacgcatcaa accgaacaac agttggcaga atattttggt aaccaacgct gtatcgttgt
    24661 ttcaggaaac agcgaagagc agaccaaagt ggctgatgca ttcggcgaag cgctgagcga
    24721 agccctagac cgactacttc cagaaggcga gaatatcatc gctgtgatgg gaggaactac
    24781 tatggctgtg gtagccgaac aattgtctaa tctggaaaat aagaaacggc ataacctgtt
    24841 tgtccctgca agaggcggga ttggtgaagc gataactgta caggcaaatt ctgtcagtgc
    24901 aagaatggct gctaaagcaa acggtaacca ccgtgcactt tatgttccgg aacaattaag
    24961 tctggcaact tacaattctt tactcaatga gccatctatc caagaagtct taaacttgat
    25021 tagtgaagcg aattgtgtta ttcatagtat tgggcgtgca ttacacatgg cagcacgtcg
    25081 taagatgact gaaaaagaat tagtcatgct gaaacaagca aatgctgttg cggaatcttt
    25141 tggctacttt ttcaatgaaa aaggtgaagt cgtttataag gtccctagaa tcggcataga
    25201 attgcaagat ttggaaaaag tcccgatcat catggcaatc gcgggcggaa aatcaaaagc
    25261 taaagcaatt cgagcttata tgaaaaatgc accaaaacaa acgtggctca tcactgatga
    25321 agctgctgca aatgagattt taaaaggggt aaccctttaa aataaatatt tttttgattt
    25381 tcataaggag gaaatcttaa atgacagtta aagtaggtat taatggtttt ggacgtattg
    25441 gacgtctagc attccgtcgt atccaagatg tagatggaat cgaagtagta gcaatcaacg
    25501 acttgacaga tgctaaaatg ttggcacact tgttaaaata cgacacaact caaggacgtt
    25561 tcaacggaac agttgaagtt catgaaggtt cattcaacgt aaacggaaaa gaagttaaag
    25621 tattagctaa ccgcaaccca gaagaattac catggggcga actaggcgta gatatcgttc
    25681 tagaatgtac tggtttcttc acttctaaat cagcagctga aaaacattta acagctggtg
    25741 ctaaacgcgt tgttatctct gctcctggcg gaaacgatgt tccaacaatc gtttataaca
    25801 caaaccacga aacattaact ggtaaagaaa cagttatctc aggtgcttca tgtactacta
    25861 actgtttagc tcctatggct aaaacattga acgacaaatt tggtgttgtt gaaggattaa
    25921 tgacaactat ccacgcttac acaggtgacc aaatgactct agacggacct catcctaaag
    25981 gtgacttccg ccgtgcacgc gctgctgctg caaacatcgt tcctaactca acaggtgctg
    26041 ctaaagctat cggtttagta attcctgaat tgaacggtaa attagacgga gctgctcaac
    26101 gtgttcctgt accaacaggt tcattaacag aattagtaac agttcttgaa aaagaagtaa
    26161 ctgttgacga aatcaatgca gcaatgaaag aagcttcaaa cgaatcttac ggatacaaca
    26221 cagacgaaat cgtttcttca gatatcgttg gtatgactta cggttcatta tttgatgcta
    26281 cacaaactaa agtaatgaca gttggcgaca aacaattagt taaaactgtt gcttggtacg
    26341 acaacgaaat gtcatacact gcacaattag tacgtacttt agaatacttc gctaacttat
    26401 aagatagaca aacgtacaag tgtaaattat agacatgtac atttaaaagc ggggaagcaa
    26461 tcgcgcttct tccgcttttt ttgataataa gtttatt
//
LOCUS       DBNICI010000019        26167 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.019, whole genome shotgun sequence.
ACCESSION   DBNICI010000019 DBNICI010000000
VERSION     DBNICI010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.019"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(130..825)
                     /locus_tag="AC1ZCC_000676"
     CDS             complement(130..825)
                     /locus_tag="AC1ZCC_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HIQ1628948.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(915..2015)
                     /gene="ychF"
                     /locus_tag="AC1ZCC_000677"
     CDS             complement(915..2015)
                     /gene="ychF"
                     /locus_tag="AC1ZCC_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIQ1628949.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(2024..2215)
                     /locus_tag="AC1ZCC_000678"
     CDS             complement(2024..2215)
                     /locus_tag="AC1ZCC_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HIQ1628950.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2236..3126)
                     /locus_tag="AC1ZCC_000679"
     CDS             complement(2236..3126)
                     /locus_tag="AC1ZCC_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIQ1628951.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3113..3880)
                     /locus_tag="AC1ZCC_000680"
     CDS             complement(3113..3880)
                     /locus_tag="AC1ZCC_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIQ1628952.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="AC1ZCC_000681"
     CDS             complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="AC1ZCC_000681"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIQ1628953.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="AC1ZCC_000682"
     CDS             complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="AC1ZCC_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HIQ1628954.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="AC1ZCC_000683"
     CDS             complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="AC1ZCC_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIQ1628955.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8451..8834
                     /locus_tag="AC1ZCC_000684"
     CDS             8451..8834
                     /locus_tag="AC1ZCC_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1628956.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(9002..9760)
                     /gene="jag"
                     /locus_tag="AC1ZCC_000685"
     CDS             complement(9002..9760)
                     /gene="jag"
                     /locus_tag="AC1ZCC_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HIQ1628957.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9778..10596)
                     /locus_tag="AC1ZCC_000686"
     CDS             complement(9778..10596)
                     /locus_tag="AC1ZCC_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HIQ1628958.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="AC1ZCC_000687"
     CDS             complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="AC1ZCC_000687"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIQ1628959.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="AC1ZCC_000688"
     CDS             complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="AC1ZCC_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIQ1628960.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11725..13059
                     /gene="dnaA"
                     /locus_tag="AC1ZCC_000689"
     CDS             11725..13059
                     /gene="dnaA"
                     /locus_tag="AC1ZCC_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIQ1628961.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13257..14387
                     /gene="dnaN"
                     /locus_tag="AC1ZCC_000690"
     CDS             13257..14387
                     /gene="dnaN"
                     /locus_tag="AC1ZCC_000690"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIQ1628962.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            14616..14858
                     /gene="yaaA"
                     /locus_tag="AC1ZCC_000691"
     CDS             14616..14858
                     /gene="yaaA"
                     /locus_tag="AC1ZCC_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HIQ1628963.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            14845..15969
                     /gene="recF"
                     /locus_tag="AC1ZCC_000692"
     CDS             14845..15969
                     /gene="recF"
                     /locus_tag="AC1ZCC_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIQ1628964.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            15966..17912
                     /gene="gyrB"
                     /locus_tag="AC1ZCC_000693"
     CDS             15966..17912
                     /gene="gyrB"
                     /locus_tag="AC1ZCC_000693"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HIQ1628965.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            17932..20403
                     /gene="gyrA"
                     /locus_tag="AC1ZCC_000694"
     CDS             17932..20403
                     /gene="gyrA"
                     /locus_tag="AC1ZCC_000694"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIQ1628966.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            20639..20938
                     /gene="rpsF"
                     /locus_tag="AC1ZCC_000695"
     CDS             20639..20938
                     /gene="rpsF"
                     /locus_tag="AC1ZCC_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIQ1628967.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            20988..21500
                     /gene="ssb"
                     /locus_tag="AC1ZCC_000696"
     CDS             20988..21500
                     /gene="ssb"
                     /locus_tag="AC1ZCC_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIQ1628968.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            21526..21762
                     /gene="rpsR"
                     /locus_tag="AC1ZCC_000697"
     CDS             21526..21762
                     /gene="rpsR"
                     /locus_tag="AC1ZCC_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIQ1628969.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            21905..23878
                     /locus_tag="AC1ZCC_000698"
     CDS             21905..23878
                     /locus_tag="AC1ZCC_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIQ1628970.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            23884..24336
                     /gene="rplI"
                     /locus_tag="AC1ZCC_000699"
     CDS             23884..24336
                     /gene="rplI"
                     /locus_tag="AC1ZCC_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIQ1628971.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            24610..25977
                     /gene="dnaB"
                     /locus_tag="AC1ZCC_000700"
     CDS             24610..25977
                     /gene="dnaB"
                     /locus_tag="AC1ZCC_000700"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIQ1628972.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         7934 a         5148 c         5192 g         7893 t
ORIGIN      
        1 tttatcgtaa aaagggactg tgacaagact tttgtcacag tcccttacta ttatttaact
       61 atggataaac aaaatgaggt tctttactac ccctagatat ctagcaaaat agctgatctt
      121 tttgctagtt tatcttcttg ggacggataa acttccaata atcccgtatt ttttcttcaa
      181 gtagtaacgg attgctaaag cagttgcccc gataatgata actactactg gatctaatac
      241 tggattgata actggtggca atagtgttgt cgcagaaaac agtacgaccc aaagaagtgt
      301 cgttccacct aggataagca atgtcttacc cattcctggt ttcttgcttc gatctgctcc
      361 gggatattca tattgataga tgtatttgta catcatataa aagacccagc caccgattgc
      421 agaacctaga acgagtgtag aaaagccata agctggcgtt gttccacgtg aaaacaatcc
      481 ggtaatcgca atcatgatac ttaatagacc aagcaacagc aacgtattat ctaaccacat
      541 caataaaggg gtggatgaat tttttacttc tgctggctta ttgatgattg cttccgttct
      601 ttcagagact gtcccaaaca attgacgggc tgttcttccg cctttttgtt ctgcaactaa
      661 agtaggcaga atgtcatgaa gggcttttac tttttcttct tcagacaaat tcgctgcttc
      721 taaagatttt ttcaaatcaa aaatgtactg ttgatttctt ttggttaatt ttgtttctaa
      781 actattgttt tcttttacga ttgctcgtaa ttgttctggt tccattatct aatagtcctc
      841 cttagccgtt ccttttctca acggtcgtca caaaagacat tctaccatat tctacagcta
      901 gtttgatagc ccttttacac attaaagcgg aataacatta cgtcgccgtc ttgtacgaca
      961 taatcttttc cttctaatcg aacacgtcca gcttcttttg ctgcatgcat gtttccgtaa
     1021 tgatccaagt cttcaaaaga gacagtttct gcacggataa atccgcgttc gaaatctgaa
     1081 tggatgattc ctgctgcttg tggtgctttg attccttttt tgaatgtcca agcacgtact
     1141 tcttgttccc cagcagtaaa gtaagtagct aagcctaaca aatcataagc agcacggatc
     1201 aactgatcca aacctgattc ttcgattcct aaagcttcta aaaattccgc tttgtcttct
     1261 tcatctaatt ctgcgatttc ttcttctgca cgagcactga ccacgatcac ttctgcattt
     1321 tcttctgcag caaagttgcg cacttcttgt acataatgat tcgcgtctgc atctgctacg
     1381 tcatcttctg agacattggc tacataaaga attggttttg tcgtcaataa aaatagtgat
     1441 ttaacgattt tttgttcttc ttcagtaaat tcgatcgttc gggcagattt tccttcttcc
     1501 agtacaggtt tgattttatc cagtacagct agttctgcca ctgcatcttt atctttggtt
     1561 ttagcaactt ttgctacacg agtatagcgt ttgttgatag attctaagtc tgctaacact
     1621 agttccaagt tgatcgtatc aatatcttct agtggatcaa cacgtccttc tacgtgagta
     1681 atattatcat catcaaaaca gcgaacaacg tgacaaatag cgtctacttg gcggatatgg
     1741 cttaagaatt gatttcccaa accttctcct ttacttgcgc cttttacgat tccagcaata
     1801 tctgtaaatt caaaagtcgt cggaacagtt tttttcggat gtacaagttc tgttaagcgt
     1861 tgcaaacgcc agtcaggtac ttctaccatc cctacgttgg ggtcgatcgt cgcaaaggga
     1921 tagtttgctg cttctgctcc tgcttttgtg atcgcattaa acaaagttga ttttcctacg
     1981 ttgggcaacc caacaattcc agcagtcaat gccataaatt tattcactct ttctctgcat
     2041 ttttttcaat tacttttttt aatttctttt caaaatctcg acgggtcatc atgacgatat
     2101 gtccgcaatt catacatctg attttgatat ctgcgcccat tcgaatgact tcccagcgat
     2161 tcgtctggca agcgtgaggt tttttcattt caacgatatc gtgcaagtcg tacataaaaa
     2221 tagcctcctt tttgtttatt cttcatcaaa ttgaatattc aagatatcaa gaattcgagt
     2281 caaatctttt tgagaaagat attcaatctc gatcttacct ttcccatttt tttcttggat
     2341 agctacactt gtaccaaatt tatccatcaa acgatcttcg ctttctcgga tataatatgg
     2401 tttttccttt gctagccttg ggattttttt cttttctttt ccatcatttt gatttaactc
     2461 agaaacaagc tgttccagtt ggcggacagt taaattttca gttactgcac gattggctaa
     2521 tttcaagatc aatgttttat tttttaaacc taacagcgta cgggcttgtc ccatagataa
     2581 acgctgatct tgtaccattt ccttgacaag atccggtaaa gaaagcaagc gcaaataatt
     2641 ggcgatataa ggtctgctct ttcctaaacg actagctact tctgcttgtg ttagtttcag
     2701 gtttttcatc agcatctcat atgcttctgc ttcttctaac ggattcagat cttctcgctg
     2761 caagttttcc agcactgcta cttgcatcat cgcttcttcg tcaaagtctc taacgatagc
     2821 agggatcgtt tctttttcag ctagtttgga cgctcgaaaa cgtcgctccc ctgcaataat
     2881 ctcatatcct tttactgcgg aacggcgtac gatgatgggt tgaaagacac ctgattgttc
     2941 aatagaatta gctagttcct gcaaagatac ttcttcaaat gttttacggg gctgatacgg
     3001 atttggtcgc agctcattca atggtagctg gacaacggta tcatctttta catccaccgt
     3061 ctctaagttt tctaaatctt gaaacagtgc atcgattcct cggcctaatc ctttactttt
     3121 cttcacgact taccacttcc tttgccaatg cttggtagac ttcagctcct cgagaacgtg
     3181 gatcgtagtc gatgatcggt tttccatgac taggtgcttc tgataaacga atatttcttg
     3241 gaatgatcgt ttcgtaaact ttttcgcgga agtatttacg tacttcttct acgacttcat
     3301 ttcccagatt tgttcgtgca tcatacattg tcagtaagac tccctcgatc tctaattctg
     3361 gattaaagtg tttttggact aaacgaaccg tattcagtaa ttggcttaat ccttccaatg
     3421 cgtaatactc acactgaaca ggtatcaaaa tcgaatcact tgctgtaaat gaattgattg
     3481 tcagatggcc aagagaaggc gggcaatcga tcaaaatata atcatactga ctgcttactt
     3541 ctgctaaaga tccttttaat cttgattctc ttgccatcat tgacgtcaat tcgatctctg
     3601 ctcctgctaa ttggagagtc gcaggcacaa tactcaaatt ttcgtgttca gtgatcaaag
     3661 ttgcctcatc aattggcaat tcattgacta gaacatcata gatgtctctt gtcacatcag
     3721 gttttctgat cccgacacca ctggtagcat ttccttgtgc atccatatcc actagcaata
     3781 cttttttgcc aaggctagca agacaagcac ctagattcac cgtcgtcgtt gttttgccga
     3841 cgccaccttt ttggttcgca acagatatta tttgtgccat ctcttctcct cctatttgat
     3901 tggctgctta ttaggcagtc cgggctttct tgggtatttc tttggggttg gtttcttttt
     3961 ttgaataacg atgatatgcc gttgatcaga gacattcggc aaggtaaccg cctcttcttt
     4021 gaggaattta ccacctaata aagcaatcgc ttgttttgct tctgtcaatt cctcttcact
     4081 ttttgctgct ttcaatgcat aaaaatagcc ttcttgttta gccaagggca aacacaattc
     4141 cgctaacaca ttcaaacgag caaccgcacg agcagtgaca aggtcaaatg atgcacgaaa
     4201 gtgaggattt tgcccaaatg tttctgcccg gtcatgatat aaggaagcat cgattcctaa
     4261 taatcgggtt aactcagata agaaggttat tcgtttattc agagaatcaa cgatcgttat
     4321 ctgcagttct ggaaaggcaa tttttaaagg tatgctagga aaccctgccc ccgaaccaac
     4381 atcgcataaa gataaaggtt gcgtaaagtc tacttcaaat gctaatgtaa gtgaatcgta
     4441 aaaatgcttc agatatacgt ctttttcatc cgtaatagct gtaaggttca tcttttcgtt
     4501 ccattcgact aatgtccgat aataaatagc gaattgattc atttgatgat cggtaagctc
     4561 tatccctttt tctgccaagg attggcgaaa ttcttctggt atcataaata ttcctttcat
     4621 tgtgaaacag caattgtttc acagattctc tccctacttt aacgcaaaat ctctagacat
     4681 gcaatccttc catatggatt cttaaaaatt tgaaaagtaa gaaaagccga tagacttttt
     4741 ctttctagta tgactcataa aatatacaca gaagagagaa ctagctttga gcaatttctg
     4801 caaaaactag ttctctcttc tatttattat tacgtattac gctggttcac tgactttcgc
     4861 aatcttccct tgttcgatat agaccattag gatacttagg tctgcagggt ttacgccact
     4921 gattcggcta gcttgggcaa ttgtttctgg ttggattttc tgcaattttt gttttgcttc
     4981 tgttgctaat ccgttgattg ctgcgtagtc gatgttttct ggaatctttt tcgcttccat
     5041 gcgttttaat ttttctactt tttccatggc ttttttgatg tagccttcat atttgatttg
     5101 gatttctact tgttcgattt cttttcgtgt caattcttca ttttctggaa taaaccgtag
     5161 aagttctgca taagtaatct caggacgtct taaaaagtcg cttgctagta caccatcttt
     5221 taatggtgcc gcattaatcg ttgctaaaaa gtcttggact tcttctgttg gtttgattcg
     5281 tacattcatc aaacgtttga tttcttgttc aactgcttct tttttcttca aataattctt
     5341 gtattgatct tcttttacta agcctacacg gtgacccatt tctgttaatc gcaaatctgc
     5401 attatcatga cgtaaaagca gacggtattc agcacgtgaa gtcagcaaac gataaggttc
     5461 gttcgttcct tttgttacca aatcatcgat catgacaccg atgtaaccgt cacttcgttt
     5521 gatcactaaa ggttcttttc cctgaacttt caaagctgca ttgataccgg caatcagccc
     5581 ttgtccagcc gcttcttcat aaccgcttgt accatttgtt tgtccagctg tataaagatt
     5641 ttcaacgact ttagtttcta atgtcggacg gagctggtgt ggctctacta cgtcatattc
     5701 gatagcatat cctgtacgca tcatttctac tttttctaat ccagcaatag aatgaagcat
     5761 gttgacttga acatcctctg gtaaagaagt agataaacct tgaacataga cttcttccgt
     5821 atttaagcct tctggttcca aaaataattg atgacgtggt ttgtctgcaa aacgcacgat
     5881 cttatcttcg atggatggac aatagcgagc gcctacccct tcaacgatcc ctgtaaacat
     5941 tggggcacga tgtaagttgt ctcgaataat ttggtgagtc gtctcacctg tataggtcag
     6001 ccagcaagat tcctgatcta gtttataggc actatctggt gtgctgaagc taaaatgatt
     6061 cggctctttg tcaccaggct gctcttctgt aacagaatag tcgatcgtac ttgattttac
     6121 tcgaggcggt gttcctgttt tgaaccgttc gatagcaaag ccaagttctt ttagatgatt
     6181 cgctagtcca atagaaggtt gagagttatt cggacctgat gaatatttca attcaccgat
     6241 aatgatttct ccgcgaagtg ctgttccggc tgtaataaca actgctttgg cccggtagct
     6301 cgctcctgtg gacgtcacta ctccatgaca aacaccatct tcgacgatca gttcttcgac
     6361 gattccttgt cttagcgtca aatttggttc tttttcgatc gtatgtttca tttctgttga
     6421 ataagcatgt ttgtctgctt gtgctcgcaa tgctcggaca gctgggcctt ttcctgtgtt
     6481 caacatacgc atttggatat aggttttatc gatattctta cccatttctc cgcctaacgc
     6541 atcgatttct cgaacgacaa cgcctttagc tggtccgcct acagaaggat tacatggcat
     6601 gaatgctacc atgtccaaat ttattgtcag taataatgtt tttgatccca tacgtgcagc
     6661 agcaagtgca gcttctgaac cggcgtgtcc tgcgccaaca acgatcacgt catacgcatc
     6721 tgcttcataa tgttccaatc gtttgttcct ccttttattt tcctaaacaa aattgactga
     6781 ataattgagt gattagctca tcttgtacac tgtcgccgac aacttctcct agatagtccc
     6841 agcaacgcgt catatctatt tgaacgagat ccacaggcat tcctgaatca atacctgcaa
     6901 tgacttcttc aagtgagagg gaagcttttt ctaacaacgc gatatggcgg gtattagaaa
     6961 cataagtagc gtctctttct cctgtttctc cgctgaagaa taaatcagaa atagctgatt
     7021 ccaaacgatc tagtccgtcg tttttagcta cggaaattga aaaaactggt tcattttcga
     7081 tcaatttctt caatttttct tgttccagct gtgctggcaa atctgtttta tttagaagaa
     7141 tgatccgttt caatccactt gtcgcttcca ataattgttc atcttctgct gtcagtggtt
     7201 cgctttggtt tagaacaagt aagatcaagt ctgcttctgc taatgctttt cggcttcttt
     7261 cgaccccgat ccgttccacc acatcttctg tttcccgaat tccagctgta tcgattagct
     7321 tgagcggtac cccccgaaca ttgacatact cttcgattac atctcgagtc gttcctgcaa
     7381 tatccgtaac gattgctttt tcttctctta acaaatggtt taacaaactt gatttgccga
     7441 catttggccg gccgatgatt gccgtactta acccttcccg cagaaccttc ccttgcttgg
     7501 atgtagcaag tagcgcttgg attctttgtt ggatcatctg cgctttttca agcaataact
     7561 tcgtagtcaa ttcttcgaca tcatcatatt ctggataatc aatatttact tctacttgtg
     7621 ctaatgtctc taaaatctct tgtctcaatg agcggatcaa agcagacaga ttgccgtcta
     7681 actgattaag agctagtccc attgctttgt ctgtttttgc ccgaatcaaa tccatcacag
     7741 cttctgcctg tgaaaggtca acacgaccat tcaagaatgc tcgttttgtg aattcgcctg
     7801 gctcagctaa tcttgcacct tctcttaaca atagctgcaa aatttgattg actacaacga
     7861 tcccaccgtg acaattgatt tctactacat cttcccgcgt aaatgtcttt ggtgctctca
     7921 tcacggagac catgacttca tcgacaagct gattgctctt gggatcaacg atatgtccat
     7981 aatggatcgt atgacttggt acttctgata atcgtttgtt tccagattga taaactttat
     8041 ctgctagttg cacagcttga tcgccactta aacgaacgat actgattgca ccttctccag
     8101 gtggggtaga aattgccgca atcgtatcaa attctaaagt aatttcagcc attttttcac
     8161 ttcctttttc attttgcgca caaaaaaagt gcccactcca ccctttatgc acaataaacg
     8221 gtgaacttcg cactttgact gcttgttctt gattaaattc tggtcataga ttatcacact
     8281 tgctataagg aatcaagtgt ttaaaacaat tagatgaagt tgagcctgtt ctgcttccat
     8341 taccttatat ttaattgtcc ttggatgtct ctttgcgttc tttggtttat tcgaagagaa
     8401 gatactgtat aaacaaggct ttatctttct taaaaaaaac ggtacactaa atggtagcgc
     8461 tatctaaact atacactttt gaaaggagaa aatatgtgag cccgttcaga tctgctgcat
     8521 tggaagaaga aagtgaccct cggatcaacg atgtcgtttt atctacaggg aatatcaacc
     8581 agaaatatat cgtccgcgat atcgtttttg ttgtggaatg cttagaagct gatttgtttg
     8641 acccatcttt tcccaaagat gaccttttct taacaacgaa gaggaaatta aagacgaagg
     8701 tacttgaata tggtgcgaat gcagtaatca actgtcattt tgagtatcag caaaaaaaac
     8761 gcgacggaaa gtattttctg gaactttttg cttatggaac agtgatccaa tttatccaaa
     8821 caacgattgg ttaaatgaaa cagacaaaaa aagcggatca aaactgaaga cagtttcgat
     8881 ccgctttttt ctatcaaagt tagtgactac ttcatcgtat actctgataa gaatgatttc
     8941 tagtaaaata tacttggttt gaaaaacttt gtcaacgtga tttattcaaa aagtaatgga
     9001 atcaataata ttttttagca ggttctacta ctaaataacg ataaggttcc tcaccttccg
     9061 aatgtgtttt tacatgttca tctttgctta atgcagcatg gatttgttta cgttcaaatg
     9121 caggcattgg ttctaagaaa acaggctgcc ctgtccgttt tactttttca gccgtgcgat
     9181 cagctaaacg ctgaatgatc tcttggcgct tttcacggta attgcctaca ttgactacta
     9241 cagacagctt atttcttgct aaacgatgga tgaagacttg tgccagatat tgcaatgcat
     9301 tcagtgtttt tccatgtttc ccaatcagta tgccttgctt ttgtgtttct aaatggaaaa
     9361 caaccatatt atttgattct ctttccattt tgacaagagc tggagcatca agctgttttg
     9421 aaatttcagt aagataaact gccaattccg ttaaagctgc ttcatcacct agctcactta
     9481 cttcttctgt tttttcttca agaatcgttt cgttgactac ctcgatctca gctgttggtt
     9541 cttcttccaa aatttcttct acagtctgtt ctactgtttc agaaactttt tcgctgatac
     9601 ttggctggat cgaaagccgt gcattctttc gacccattcc taggaaacct ttttttcctt
     9661 cttccagtat ttcgatttcg acttggtctt tggccaaacc caaatcagcc agacctagtt
     9721 ggacagcttc gtctattgtt gcagcttcat aaatcggcat tagcattacc tcccctatta
     9781 tttccgtttt ttcttaggat tcattgcttt cttaagagca cgttctcttt cgcggattct
     9841 ccgtgcttct tcttcacgct cttttcgaat tttgaaaggg ttgttgatca gcagcgtttg
     9901 tacaacttgg aaggcattgg aaactaccca gtataatgaa agaccgctgg ctaaattgac
     9961 acccatcact aaaatcatta caggcatcac gaaattcatg atcttcattg atgcattcga
    10021 ttcaatttga ctcatgctag ataaatacgt acttgcaaaa gtaaagattg ctgctaaaat
    10081 cggcaaaata aatgttggat ccgcgttccc taagttcaac cataagaaat gcccttgttt
    10141 caactctggt acacgtgaaa tagattgcca taacgccatc aagattggca tttgtactaa
    10201 taaaggaaga caaccagcat atggattgac gccattttct gcatataaac gctgctgctc
    10261 ttctcttaac ttactttgtg tttccggatc tcttgaacta tattgttgct gcagtgcttt
    10321 taatttcggt tgtaattctt gcgttttacg catgctcttt gtttggaaat gcatcaacgg
    10381 caataggatc acgcggatga tcagtgtaaa taaaatgatc cctatccctg cattaccaaa
    10441 tgacaacgcc ttgatcgcct cagcaaagta ataaacaata taacgatccc aaattcctgt
    10501 actctgtgcg ctgacttccc cagttccgca accggataag acgatgacca aactaacgat
    10561 accggccatc aaaagtaaac gtttgtattt cttcacttac tcttttcctc tctgtcgatt
    10621 atttttgcaa gttttaagac gtggactaaa ttggagcgaa cctcatcata agtcaacccc
    10681 ttcacacttg gtctagcaat cacaataaaa tccaactccg gatcaatttc ttcttttata
    10741 gaataaactg ctgctcggat ttttcgtttg acttcattgc ggtttaccgc gttgccgacc
    10801 ttttttccta cagataaacc aactcgaaaa tgaggctgtc ccggtttttc caaacggtaa
    10861 acgacgaatt tgcggttagc aaaagaatct cccgtatgga acacggcttg aaactctcgt
    10921 tcttttttta ctcgatacgc ttttctcatt agttttcttt ccttttctat ctctcactta
    10981 cgctcataat cataccataa ttacgattgt cctttcatca tttccctaaa aaagagtata
    11041 aaaaaaacca ctgaaacccc agtggcctaa gctgaaagaa cttttcttcc tttgcgacga
    11101 cggctagcta acacacgacg tccattttta gtactcatac gtttacggaa tccgtgaact
    11161 ttttgacgtt tacgtttatt tggttggtat gttcttttca tatattccac ctccaaaatg
    11221 ttcttatcta ttttataaac acagacatac tttgatagta taacgacaaa gtagtgattt
    11281 tgtcaatata aaatagaaga tcgattgtta aaacttatcc acaagattat ttttttctat
    11341 gggagtccta tgtggaaaaa gttatgcaca gggattatcc acagataata aatggctggt
    11401 tttacacaga aaacacacgt gtaaaaaaag taatccacaa tcaggtgatt tcctgtgtat
    11461 tccgtctcct aactattggt atatctatat tttttatcca cagatgcaag aggaaatcta
    11521 ttgattttta cgagttttcc acctgtgcat tagtggtgtg gaaaactttt ttttgacaac
    11581 tgtgtttttc tcttttttct ttttgtcgat tgtgtggata attgtaatta aaaaattttt
    11641 ttcttcggtt tctgtggaaa actattgaaa aaaatgctaa attagtacta gttattcact
    11701 ttttaatgaa ggaggaggcc ttatatggta tccctcgatg ctttatggaa tgaattaaaa
    11761 gcaacatacc aaaaggattt atcgcctgct agctataata catggatcga aacagcgaac
    11821 cctcgaaccc ttgatcaaaa ccagctcgtt gttgaggttc ctagcaagat tcataaagaa
    11881 tattgggaaa aaaacctggc gacaaaaatc gttgaagtcg gttatatgtt atcaggaaat
    11941 gagattatcc ctcgctttat tactggcgaa gaagcagagc aagaagaagt tatagaagaa
    12001 aaaaatccaa aagttgtggc acctagtccg ctaaaaaaag ccatgctgaa ccctaaatat
    12061 acctttgata cgtttgtcat tggtaaagga aaccagatgg ctcatgcagc agcgctcgtt
    12121 gtagcagaag atccaggttc tatttataac ccgctcttct tttatggagg cgtgggactt
    12181 ggtaagactc acttgatgca tgcaattggt catcaaatgc tgcaaagtca gcctaatgcc
    12241 aaagtaaaat atgtaagcag tgaaactttt gctaatgatt tcatcaattc gatccaaaac
    12301 aaaacagcag aagagttccg tcaagaatat cgaaatgttg atttactgct agtggatgac
    12361 attcaatttt ttgcagaaaa agaagctacg caagaagagt ttttccatac tttcaatgcc
    12421 ttgtacaatg aagggaaaca aatcgtacta accagtgatc ggttgccaaa cgagattccg
    12481 aaactgcaag aacgcctcgt ttctcgcttt gcatgggggc tgtctgttga tattacaccg
    12541 ccggatcttg aaacaagaac agcgatttta cgtaaaaaag cggcggctga acgtctagag
    12601 atcccagatg acacactaag ctacatcgca ggacaaattg attctaacat tcgtgaatta
    12661 gaaggtgcgc ttgttcgagt gcaagctttt gctgcgatga atagtgaaga tatttcgact
    12721 agtctagctg cagacgcact gaaaacgtta aaatcaggaa aaggacatcc tcaattgtca
    12781 atcctgcaga tccaagaaga agtggcaaag tattatcaca tccaattgaa agatatcaaa
    12841 ggaaagaaac gcgtcaaatc tattgttgtt cctagacaaa tcgctatgta tttggcacgt
    12901 gagctcacag ataattcttt accgaaaatc ggagctgaat tcggtggcaa ggatcataca
    12961 acggttatcc atgctcatga aaaaatccag cagctgatgg attctagtgt cagtatgcaa
    13021 aatgaagtat cagaaatcaa aaacttttta ctaaattaat ttgtcgattc tatcaactga
    13081 aaatagcatg tggataaaag aaaaaaacgt tcaaaagtta tgcacacttt ttccacagct
    13141 gttttttcgt ttctctctta cgttttttga gttttccaca gaatcaacat gccctattac
    13201 ttttattatt ctttttaact aataataaat aaacaaacat tcaaaggaga tacatcatga
    13261 aagttacttt aaaccgagct agctttatgc aggaattgca aactgttcaa cgagctattt
    13321 caagcaaaac cacgatccct attttgacag gtgtaaaaat cacactgaca caagaaggtt
    13381 tgactttgac ggggagcaac gctgatatat caattgaaac ttttttgtct gttgaaaacg
    13441 aaaaagcaaa tatgcaaatc gaatctactg gttccattgt tttacaagca cgtttcttta
    13501 gcgaaatcat tcggagactt cctgaagaaa catttacttt agaagtttta gaaaataaac
    13561 aagtagcgat tacttctgga aaagcgaatt ttatcgtaaa tggattagat gcagataact
    13621 atcctcatct tcctgttgtc gaaagccata accagatgaa attacctgta cacgtattga
    13681 ctaaactaat caacgaaaca gtttttgctg tctcacaaca tgagagtcgt ccaatcttga
    13741 caggtgtgca tttcatttta tctgataatt ctttattggc tgtagctact gattctcacc
    13801 gtctaagtca acgcgtgatt ccagtagaac aagcggctga tcattttgat attgttattc
    13861 ctggaaaaag tttgatcgaa ttatctcgct cattaacaaa tgaagaagaa atcgtcgaaa
    13921 tcagcattat ggaaaaccaa gtgttgttca aaacagaaac gatgtatttc tattcccgtt
    13981 tgctagaagg aaactatcct gataccaatc gtttgattcc ttcaagtttt aatacagagg
    14041 ttgaattttc tgttcctagc tttttagcgg cgatcgaacg ggcttcttta ctttctcatg
    14101 aaggccgtaa caacatcgtt cgtttatcta ttcgtccaga tgcggttgtt ttatacggaa
    14161 attcgccaga aatcgggaaa gtagaagaaa gcttgagtta tacagcaagc agtggcgatc
    14221 cattagatat ttctttcaat ccggattata tgaaagcagc gttgcgtgca tttggagata
    14281 tgagcatcaa agtgaaattt atctctgcta ttcgtccatt tacattggaa ccaacggaag
    14341 atggcgttca atttattcag ctgattactc ctgtacgtac aaattaacaa tgattttcat
    14401 tgttcttgaa aatttaattg aaaagaaata gaaaaaataa aattttaaac agaaggccct
    14461 aaaatctctt ttaaggcttt ctgtttattt ttgtttaaat cacctttttt agattaaatc
    14521 gtcttatatg gaaaaatata tggataaatt tgttttattg cgaaaaaaaa ggtataatag
    14581 agtatacttt taataatgaa aatgagggat gggcattgaa acaacaagtc attctaaata
    14641 cggaattcat gacgttgggc caaatgttga aagaagtcac agttatcggt agtggcggac
    14701 aagcaaaatg gtatttggca gaaaacactg tgttagtcga tggcgaacca gaaaatcgtc
    14761 gtggacgcaa actttatccc ggcatgatgg ttgaagttcc agaagtagga acttttttta
    14821 tggtaaaaaa aggaaacgac gataatgagg ctgaataagc tgtatttaaa aaattatcga
    14881 aattacgaag agctgaacat cgaattttcc aagaacctcg tcatcttttt aggagaaaat
    14941 gcgcaaggaa aaacgaatct tttggaaagt atctacgtat tagctatgac gcgaagtcat
    15001 cggacaagta gtgagcagga attgattggc tggactgacg atcaggcgat gattcaagga
    15061 gagatcacaa aaggttcatc cactcttcca ttagaaatct tattatctaa aaaaggccga
    15121 aaaaccaaga tcaaccatat cgaacaaaaa aagttaagta cttacgtggg acaaatgaat
    15181 gtcattttat ttgcaccaga agatctttct ttagtaaaag gcagcccgca gatcaggcgg
    15241 aagtttttag atatggaatt aggccagatc aatccaatct atctttatga tcttgtccaa
    15301 taccaagcaa ttttgaaaca aagaaaccag tatttaaagc aattgaatga aaaaaaacaa
    15361 actgatttgc tttatttgga tattttgtcc gaacaactag cagcgtttgg cagcaaagta
    15421 ttgaaagcaa gagcacagtt cgtcaagcga ttggagtttt gggctaattc tttgcatcag
    15481 caaatcaccc atcaaaaaga gcagctggaa atcgagtatc tcaccgcagt cgattcttta
    15541 gaaacacata cgcaagagca aatccaagaa caatttctcg cacttttgaa ccaaaataaa
    15601 aagaaagacc tttttcgggg aacaacgaca gttggtcctc atcgtgacga tctttccttt
    15661 tttattaatc agaaaaatgt gcagacttat ggatcgcaag gacagcaaag aacaactgca
    15721 ctaagtgtca aattagcaga aatcgatttg attaaagaag agacggggga atatccaatc
    15781 cttttgttag atgatgtgat gagcgagctg gatgatagtc gtcaactcca tctgctagaa
    15841 acaatcgaag gaaaagttca aacgttctta acaacaacga cacttgacca tgtaaaaaat
    15901 aaaatgactg ttgaacctga gattttttat gttcaacaag ggaaactaaa aggagaagaa
    15961 actgaatgac agaagaaaga agtttagtag aacgcgctaa agagtatgat gccagtcaga
    16021 ttcaagtact tgaaggactt gaagccgttc gtaaacgacc tggcatgtat atcggttcga
    16081 ctagttctga gggtctgcat caccttgttt gggaaattgt cgataattcg atcgatgaag
    16141 tattagcagg ttttgcaacg aaaatccatg tgattattga aaaagacaac agtatcaccg
    16201 ttattgacga cggtcggggg atacctgtag atatccaggc aaaaactgga cgtcctgcag
    16261 ttgaaacggt ctttactgtt ttacatgctg gaggaaaatt cggcggtggt gggtacaaag
    16321 tttctggtgg acttcacgga gtaggatctt ctgttgttaa cgcgctttct acacagcttg
    16381 atgtcaaagt atacaaagaa ggaaatgtgt actatcaaga atatcgtcgt ggagctgtag
    16441 tggacgactt aaagattatc gaacaaacgg atcgccatgg aacaacggtt cactttacgc
    16501 cagaccctga aatttttaca gagacaactg agtttgattt ttcaaaattg gctacccgga
    16561 tccgtgaact tgctttcttg aaccgcggga tgcgtatatc gattgaagac aaacgagaag
    16621 aagaaccagt catcaatgaa taccattatg acggcgggat taaaagttat gtcgaatact
    16681 tgaatgcaaa caaaaccgtg atttttccag aacctgtata tttggaaggt gaacagcaag
    16741 atatcgcagt tgaagtctct atgcaatata cagacggcta ccattccaat atcatgagtt
    16801 ttgccaacaa tatccatacg tatgaaggcg gaacacatga atcaggattc aagactgctt
    16861 taactcgtgt gataaatgat tatgcccgaa aacaaaagct gatgaaagaa aatgacgaca
    16921 acttgaccgg tgaagacgtg cgagaaggat tgacagctgt tatttctatc aagcatcctg
    16981 accctcaatt cgaaggacaa acaaaaacaa aattgggcaa ttccgaagta cggacagtca
    17041 ctgatcggtt atttagcgaa catttcatga aattcttgct ggaaaaccca agtgtgggcc
    17101 gtcaaattgt tgaaaaagga ttactagctt ccagagctcg tctagcagcg aaacgtgcgc
    17161 gtgaagtaac gagacgtaaa ggtgctttag agattagtaa tctgccagga aaattagcgg
    17221 actgctctag caacgatcct gaaaaatgcg aattgttcat cgtcgaagga gattcggccg
    17281 gcggatcagc taagcaaggt cgtaatcgag agtttcaagc catcttgcct attcgtggga
    17341 aaatcttgaa tgttgaaaaa gcaagcatgg acaagatctt ggcaaatgaa gagatccggt
    17401 ctttatttac tgctatgggt acagggtttg gagaagattt tgatgtatcg aaagctcgct
    17461 accacaaatt agttatcatg actgatgccg atgtcgacgg cgcgcatatc cgtacattgc
    17521 tgctgactct tttttatcgt tacatgcgtc ctattgttga agctggatac gtctatattg
    17581 ctcagccacc tttatacggg gtaaaacaag ggaagaatat tacgtatgta caaccaggaa
    17641 aaaatgcaga agaagagttg aaacaagtcg ttgcttcact tcctgcctca ccaaaaccaa
    17701 gcgttcagcg ttataaagga cttggagaaa tggatgatca tcaactatgg gaaacaacta
    17761 tggacccaag caatcggatg atggcccgcg tcagtgtgga cgatgctatt gcagcagatc
    17821 agatatttga aatgttgatg ggggatcgag tagaaccccg tcgtgcattc atcgaggaaa
    17881 atgcacacta tgtgaagaac ttagatattt aatcatgaag gaggaaaaag aatgagtgaa
    17941 gaaatcagag aaaacatcca tgatgtcaat ctaaccagcg aaatgaaaga ctccttcatc
    18001 gattatgcga tgagtgttat tgtcgcacgt gcacttcctg atgtcagaga tggtctgaaa
    18061 cccgttcatc gccgtatttt atacggcatg aacgaattag gtgtaacacc ggataaagcc
    18121 cataaaaaat ctgccagaat cgtcggagat gtcatgggta aataccatcc tcatggagac
    18181 tatgcgatct atgagtcaat ggtacggatg gcacagccat tcagctaccg ttatatgcta
    18241 gtggatggtc atggaaactt tggttctgtt gacggtgacg gtgccgccgc tatgcgttat
    18301 accgaagcac ggatgagcaa aatcgctaca gaaatgctgc gtgatatcaa taagaacact
    18361 gttgatttcc aaagcaacta tgatgataca gaaaaagaac cagttgtact acctgctcgc
    18421 ttcccgaatc ttttagtgaa cggaacaacg gggatcgccg taggtatggc cacgaatatc
    18481 ccacctcata acttgtctga ggtagtagcg gctatcgatt tgctgatgga taatcctgat
    18541 gtgacgacca atgaattgat ggaagtatta cctggaccag attttccaac aggtggtttg
    18601 gttatgggaa aatcaggaat ccgaagagcg tatgaaacag gtaagggttc tattaccgta
    18661 cgtgcaaaag ttgagattac tgagatgcca aatggtaaag aacggatcat cgtgacagaa
    18721 ttgccttata tggtcaataa agcaaaattg atcgaacgga tttctgaact gcacagagac
    18781 aagcgtatcg aagggatcac agatttgcgg gacgaatctt cccgtgaagg tatgcggatc
    18841 gtcattgaca tccgtcgtga cgccagcgca tctgttatct tgaataactt atataaattg
    18901 acatccttgc aaaattcatt tggattcaat atgttggcga tcgaaaaagg tgtgccgaaa
    18961 gttttgagct tgaaacaaat tcttgaaaac tatatcgaac atcagcgcga agtcatcaca
    19021 agacggacag tctttgaaaa agaaaaagca gaagcaaggg cacacatttt agaaggtttg
    19081 cgtattgctt tggatcatat cgatgaaatc attgcgatta tccgcgggtc taaatcagat
    19141 gacgaagcaa aagcgacgat gatcgaacgg ttcgatttat ccgatcgcca gtctcaagct
    19201 attttagata tgcgtttacg ccgattgacc ggtttggaac gggaaaaaat cgaaaatgaa
    19261 taccaagaac tcttaaaact gattgaagat ttaactgaca ttctggcaag accagaacgt
    19321 gttacagaaa tcatcaagaa tgaattagca gacttgaatc aacgatttgg tgataaacga
    19381 cgcacagaat tactagtcgg tgaggtctta agtctggaag atgaagattt aatcgaagaa
    19441 gaagaaatcg ttattacatt gacaaacaat ggttacatca aacgtttggc aaataatgaa
    19501 ttcagagctc aacgaagggg tggacgtggt gtccaaggaa tgggtatcca caatgacgac
    19561 ttcgtcaaaa atctggtttc ttgttctacc catgatacat tgttattctt tacgaataac
    19621 ggaaaagtgt atcgggcaaa aggatacgaa attccagaat acgggcgcac agccaaagga
    19681 ataccaatta tcaatttact aggtatcgat tcgagtgaaa gcatccaggc aatcattgcg
    19741 gtgacgggag aagcagaaga aggccactat ctcttcttta cgacgcgtca aggtacagta
    19801 aaacgtactt ctgtcactgc ttttgctaac atccgaagta atggtctgat cgcaattggt
    19861 ttgaaagaag acgatgagct cgtcaatgtg ctactgacag atggtcaatc taatatcatt
    19921 attggaacac ataacggcta ctctgtcact ttcaaagaag aagcagtccg cgatatggga
    19981 cgtacagctt ccggggttag aggaatacgc ctgcgtgagg aagattatgt cgttggggca
    20041 gccttaatga aggaagatca agaagtatta gtgatcacag aaaaaggtta cggaaaacgg
    20101 acaaaagtat cggaatatcc agtgaaaggc cgcggaggta aagggatcaa aacggcgaac
    20161 atcactgaaa aaaatggtcc actagctgga ttgaccactg tttctggaga tgaagacatc
    20221 atgctgatta cagataaagg tgtcattatc cgtttcaacg tatcgactgt ttctcaaact
    20281 ggacgttcaa cattaggcgt acgtttgatg aagatggaag ctgatacgaa agttgtcacg
    20341 atggcagcag tcgaaccaga agctgcagaa caaacagttg aagaacaagg atcagaagaa
    20401 tagccatcaa gactaattgt gtaagctagc aagaatgacc gattaaaaaa tctggttatt
    20461 cctgctggct tttttgatta gttgaatttc tcttaaaata ttcgaaactg ttcattctgt
    20521 attgcttttg accaaaatag agtgtataat cagagcttgt gagtagtcta attaattaga
    20581 taactctcct tgctcttagg aacctaagag ccgaaagtcc acagggaggt gaatatcaat
    20641 ggaaaacacg aaatatgaaa tcatgtacat tattcgtcca aacattgatg aagaagcaaa
    20701 aactgcttta atcgaacgtt tcgatgcaat cttgaaagat aacggagcag aagttatcga
    20761 atcaaaagat tgggaaaaac gccgtttcgc ttatgaaatg aacggttacc gtgaaggtat
    20821 ctatcacatc gttaaagtat cttctccatc atcagcaaat gctgtcaatg aatttgaccg
    20881 tcttgctaaa atcaacgacg atatcattcg tcacatgatt gttaaagaag aagcttaatt
    20941 gttgtttcat gtgaaacaat agaaaacgat gagaggagat gactattttg attaataatg
    21001 ttgtattagt tggacgtctg acgaaagatc cagatttgcg ttacacagca agtggtacgg
    21061 cagtcgcaac atttactttg gctgtgaatc gtaattttac aaaccaaagc ggcaatcgcg
    21121 aggctgattt tatcaactgt gtgatttggc gcaaatcagc agaaacttta gcaaactatg
    21181 ctcgtaaagg aacactttta ggtgttactg gacgtatcca aactcgttct tatgataatc
    21241 agcaaggaca acgtgtatac gtgacagaag tagtagctga caacttccaa ttgctggaaa
    21301 gtcgttctgc atctgaaaat cgccaacaaa gcggcggatt ccaaagttct ggtcaatcag
    21361 ctggcggatt tggcggaaac aataactcta atcaaacatc tcaatcatcc aacggtatgc
    21421 ctgatttcga ccgtgatact tctgacccat tcggctcaag ttcaacgatc gacatttcag
    21481 acgatgattt accattctaa acgaatttga taggagggaa atagaatggc acaacaaaga
    21541 agaggcggac gtaaacgtcg taaagtagac tacattgctg ctaaccatat tgaatatatc
    21601 gattataaag atatcgaatt actaaaaaga tttatttcag aacgcggtaa aatcttgcca
    21661 cgtcgtgtaa ctggtacagg cgctaaaaac caacgtaaaa ttactgttgc aatcaaacgc
    21721 gctcgtatca tgggcttgct acctttcgtt agcgaagact aataaatcct aggaattgaa
    21781 tgactgatcc ttttgatcag ctattcaatt cctttttttc tgtaaaaata acctctattc
    21841 gtttagtcgc tcgaatgacg aaagttatgg taaaatcaaa ttagtcatat atgggaggaa
    21901 aattatggaa aataaaaaca atcatttatc cccgaaacct ctcttcctta tcctgtttat
    21961 attaatcgaa gtattggtag tcttaatttt accatttcgt tttgttagcg cttcggtcat
    22021 tctattgatg aatttatggg gtgttcgcta ttggttgaaa atgaaacgtt ggcttatttt
    22081 aaataaacgt gaaaaaatcc agcaggcttc gactgtagct gaaaccaatc tagcttatgt
    22141 ctcagaagtc atgcctgtag gagtgattgc ttatcagcca tcaagccaaa aaatcgaatg
    22201 gctcaatcca ttagcattga aaatcaaaga tcaaagtgga ttaacagagg aagaattgct
    22261 agatactttt tttactgcaa aagaaaaaaa gaaaaaccaa gtctatctag cagataaaac
    22321 atttcagttt gatatggata cagtaaaggg tgttgtttat tttaatgata ttacccaaaa
    22381 ccagttgtta aaactccatc agacgaacag tcagccggtg attggtattt tatctattga
    22441 taactatgca gatgccattg ataaattgga tgacaaagag atttcttatt tgaactcttt
    22501 cgtcactaca ctagtttctg actggatgaa tgaataccat gtctttttca aaagaatcaa
    22561 cgcagaacgg tttttcttcg tagcacagat gatcgatctg aaaaagatga tggataaaaa
    22621 atttgatttg ctggatcgct ttcgagcaga agctgaaaaa cagagctcgc ctatcaccct
    22681 tagtatgggg attgcttatg gctcaagaac cctcgatgaa atcggtggag aagctcagaa
    22741 taatttagat gttgctttag tccgtggagg cgaccaagtt gtcgtgaaag aggtaaaaga
    22801 aggagcaaaa cctgtttatt atggggggaa atctgctaag aatgtcaaac gcacgcgtgt
    22861 tcgttctcga gcgatgagta ccgctttgcg tcggattttt gatgaaacag gcgatgtgtt
    22921 tatcatgggg cacaagtttc cggatatgga tgctttaggc tctgcttttg gtgtcagttg
    22981 tctggcacgc ttcaaccaga aaaaagctta catcgttatc aacgagaacg agatcattcc
    23041 agatgtagaa cggtgtcttg aagaaatcca taagcatcct gaattagaaa tgcaactaat
    23101 ctctccagaa cgtgccatag agctgaaaaa agaagaggac ctcctcgtga tggtggatta
    23161 ccataaacct tctctatcga tctctcaggg cctatatgag caatttgata agattgtgat
    23221 cattgatcac catcgtcgtg gggaagagtt tccaagtaaa cctctgctga cttatatcga
    23281 atcttcagct tcttcagctt cagagctagt ttctgagctg atccaatacc aaagcagcaa
    23341 aacaaatcgc ttagatcgtt ttgaggccac attattgctt gcagggatgg ttgtagatac
    23401 aaatagtttc aatgtccgta caactgcacg aacttttgac gttgccagct atttaaggtc
    23461 ttgtggagca gacgcatcct tagtacaata tttattgagc tcggatttag cgacatactt
    23521 ggagatcagc aatttgattg ctggaagcga ctatgtgact tctgatattg ttgtggcggc
    23581 agggacagac gaaaaagagt ataatagtgt tatagcagca aaaacagcag atacattgct
    23641 ttcgatgatc aatatcaatg cagctttcgt cattacaaag cggacagacg gcttgattgg
    23701 gatcagtgcc agaagtaccg gcacgatcaa cgtccaaatc atcatggaat ccttaggtgg
    23761 cggaggacac ttcacaaacg cagctactca actggaaaat acgacagttg aggaaacaaa
    23821 acagaagcta tttgctgcaa tcaaagaaaa tatgaatcaa atatatgata aggagtaatc
    23881 caaatgaaag taattttttt acaagatgta aaaggaaaag gtaaaaaagg ggacatcaaa
    23941 gaagttccag caggttatgc acaaaattat ctaatcaaaa ataacttggc aaaagaagca
    24001 acgaaaagca gtatcagtca gttagcagga caaaagaaag cacaagaaaa acacgaagct
    24061 gaaatcttgg aagaagctaa aaagttaaaa gaattcttgg aaaaagaaga tactgtagtc
    24121 gaattgaagg cgaaagcagg ggaagacagc cgtttgtttg gttcgatccc ttcaaaacaa
    24181 attgctgaag ggttaaacaa acaatacaaa atcaaattag ataaacgaaa aatcgaatta
    24241 gaaaatccga tccgttcact aggctacaca aacgtacctg ttaagcttca tcatgaggtg
    24301 acggctacga tcaaagttca tgtcgtacaa gaataacaaa tcttgatagc gatgttcagc
    24361 tggtaagtat aaccagaagg aaaaaagaca aaccgaaatt tatctgagaa aacagctgca
    24421 gcaatgaaag caaaagctgc aaatcgattt cagattgatt tcggtttctt ttttgggaac
    24481 agcaaaatag cataaagaaa acgagtataa taaaagagaa ctattttttt attacacttt
    24541 cggttcgaat gatgactttt ctctcatttt cttgtaaaat gaagcaattg aagacaagga
    24601 gcgatacgta tgaatgaagt gtggcaggat cgaatcccac cgcaaaatat tgaagctgaa
    24661 caagcggttt taggctctgt ttttcttgac gcagaaacga tcattgatgc catggagtac
    24721 atagagccaa aagactttta tcgtcgtggc catcaaatta tttttgagac aatgatcgag
    24781 ttaaatgatc gtaatgaagc gattgacgtg gttactgtca aagaccggct ggaacaagcc
    24841 aatttgttag aagatgccgg tggtttaagt tatctctcag acttggcatt agcagttccg
    24901 acagcagcaa atatcgtcta ctatgcgaaa atcgttgaag aaaaatcatt gctgcgtaca
    24961 ttgatccaga cagcaacagg aatcgttacg aaaggattcg agcaaggaga agaagttcaa
    25021 acaattttag atgatgccga acgcagtatc ttggaggttt cggaaaaaag aaatcgaagc
    25081 ggctttttat ctattgctga agtactgaac tccacgattg aaaatatcga tcgactagct
    25141 caaaacaatg aagaaatcac aggtctacca actggctatc aaggtctgga taaaatgaca
    25201 gcaggtttcc agcccgaaga actaatcatt cttgctgcac gtccagcggt aggtaaaaca
    25261 gcgtttgcat tgaatattgc tcaaaatgta ggaacaaaaa cagatcgttc tgttgcgata
    25321 tttagtttgg aaatgggggc agaatctctt gtaaatcgga tgctttgtgc agaaggttcg
    25381 attgaggcca gccatttacg tacaggtcag ctttcggaag aagaatggtc gaatctgatc
    25441 gttgcgatgg ggagtctttc tcgagcaaat atttttatcg atgatacacc agggatcaaa
    25501 atctctgaaa tccgtgcgaa atgtcggaaa ctagcgcaag aaaaaggcaa tctaggcttg
    25561 atattgatcg attatttgca gttgattgaa ggtaacggta aagagaaccg tcaacaagaa
    25621 gtatcagata tctctcgcca actgaaaaaa ctggcgaaag agctgaaagt acctgtcatt
    25681 gctctttcac agttatctcg tggagtagaa cagcgacaag acaaacgacc agtgctaagt
    25741 gatattcgtg aatccggatc aattgaacaa gatgcggaca tcgtagcctt cttgtatcga
    25801 gatgattatt atgaaagaga aggcggagat gacgatgatg gaaacgagcc aaaaaatgac
    25861 aacgtcatcg aagtcatcat cgagaagaac cgaagcggtg cacgaggaac agttgaacta
    25921 ctgtttatca aagaatacaa taagttttcg tcgctttcgc caagagaaga attttaaaga
    25981 agaggttgtg agccttgagc aatcccgaga gctctcgtaa gaagatgcaa ggagtaaccg
    26041 caggaacgac tagagtactt aatttaaaca gatctccaaa gggattgtga caaaagtctt
    26101 gtcacagtcc ctttttccga ataaacggtg ttcaatcagc tgacccttgg tattagctca
    26161 gcaaacg
//
LOCUS       DBNICI010000020        25574 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.020, whole genome shotgun sequence.
ACCESSION   DBNICI010000020 DBNICI010000000
VERSION     DBNICI010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 25574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.020"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            548..3187
                     /locus_tag="AC1ZCC_000701"
     CDS             548..3187
                     /locus_tag="AC1ZCC_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIQ1628973.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(3355..4053)
                     /locus_tag="AC1ZCC_000702"
     CDS             complement(3355..4053)
                     /locus_tag="AC1ZCC_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HIQ1628974.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            4271..5416
                     /gene="tgt"
                     /locus_tag="AC1ZCC_000703"
     CDS             4271..5416
                     /gene="tgt"
                     /locus_tag="AC1ZCC_000703"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIQ1628975.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            5513..5893
                     /gene="yajC"
                     /locus_tag="AC1ZCC_000704"
     CDS             5513..5893
                     /gene="yajC"
                     /locus_tag="AC1ZCC_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIQ1628976.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            6198..8795
                     /gene="adhE"
                     /locus_tag="AC1ZCC_000705"
                     /gene_synonym="adhC"
     CDS             6198..8795
                     /gene="adhE"
                     /locus_tag="AC1ZCC_000705"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HIQ1628977.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            9024..10130
                     /locus_tag="AC1ZCC_000706"
     CDS             9024..10130
                     /locus_tag="AC1ZCC_000706"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88/105 protein"
                     /protein_id="HIQ1628978.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            10147..11175
                     /locus_tag="AC1ZCC_000707"
     CDS             10147..11175
                     /locus_tag="AC1ZCC_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIQ1628979.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            11181..11660
                     /locus_tag="AC1ZCC_000708"
     CDS             11181..11660
                     /locus_tag="AC1ZCC_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIQ1628980.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            11676..12971
                     /locus_tag="AC1ZCC_000709"
     CDS             11676..12971
                     /locus_tag="AC1ZCC_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIQ1628981.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            12982..13806
                     /locus_tag="AC1ZCC_000710"
     CDS             12982..13806
                     /locus_tag="AC1ZCC_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIQ1628982.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            13971..14870
                     /locus_tag="AC1ZCC_000711"
     CDS             13971..14870
                     /locus_tag="AC1ZCC_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1628983.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(14996..15865)
                     /locus_tag="AC1ZCC_000712"
     CDS             complement(14996..15865)
                     /locus_tag="AC1ZCC_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIQ1628984.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            16018..17478
                     /gene="rhaB"
                     /locus_tag="AC1ZCC_000713"
     CDS             16018..17478
                     /gene="rhaB"
                     /locus_tag="AC1ZCC_000713"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HIQ1628985.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            17475..18731
                     /gene="rhaA"
                     /locus_tag="AC1ZCC_000714"
     CDS             17475..18731
                     /gene="rhaA"
                     /locus_tag="AC1ZCC_000714"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HIQ1628986.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            18745..19590
                     /gene="rhaD"
                     /locus_tag="AC1ZCC_000715"
     CDS             18745..19590
                     /gene="rhaD"
                     /locus_tag="AC1ZCC_000715"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HIQ1628987.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            19592..19903
                     /gene="rhaM"
                     /locus_tag="AC1ZCC_000716"
     CDS             19592..19903
                     /gene="rhaM"
                     /locus_tag="AC1ZCC_000716"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HIQ1628988.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            19985..21136
                     /gene="fucO"
                     /locus_tag="AC1ZCC_000717"
     CDS             19985..21136
                     /gene="fucO"
                     /locus_tag="AC1ZCC_000717"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HIQ1628989.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            21263..22021
                     /locus_tag="AC1ZCC_000718"
     CDS             21263..22021
                     /locus_tag="AC1ZCC_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIQ1628990.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            22121..22765
                     /locus_tag="AC1ZCC_000719"
     CDS             22121..22765
                     /locus_tag="AC1ZCC_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HIQ1628991.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            22772..23779
                     /locus_tag="AC1ZCC_000720"
     CDS             22772..23779
                     /locus_tag="AC1ZCC_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIQ1628992.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            23795..24637
                     /gene="kduI"
                     /locus_tag="AC1ZCC_000721"
     CDS             23795..24637
                     /gene="kduI"
                     /locus_tag="AC1ZCC_000721"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HIQ1628993.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            24657..25460
                     /locus_tag="AC1ZCC_000722"
     CDS             24657..25460
                     /locus_tag="AC1ZCC_000722"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HIQ1628994.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
BASE COUNT         8283 a         4493 c         5721 g         7077 t
ORIGIN      
        1 gctctcgata agctcggctt tcttgtacct cggaaggatt caaataaaca gaaatgattg
       61 taactttcca tcatacatgc aaagcacggt gttgaatttc ttcatgttaa acaaaaattt
      121 tgttttcgtt acatcttcaa catttgaaac atctcttgtt cctattcaat tttcagacgt
      181 ttacgttatt caattgttac gtatcgcatt acttcttgtt tcggatactt cgtgatccaa
      241 acatccatac gcattcacta tttataggtt tgtactctac acgcatgtct gggtgaaacc
      301 tgtacttcta acaactttgt ttgcttacac tgttctatcg agaaattctt ctgtctattt
      361 gatggtatcc tccttcctta catctatata atagcatcct ttggtagcgt ttgcaaatat
      421 aagcacttta aattttacgc tttcttaaca aataatcgat aagcccttta tttgaagtaa
      481 ataaaaaaag gatgttatag taaaataagt accttttttt gtacgaagat tttaaaggag
      541 gaataggatg gaagaataca agcagacaat agaagaagta aagaaggtaa cagaagcaga
      601 tccagatact gggttgtctt cacaagaagt ggatgaacgg cgaaaaaagc agggattgaa
      661 taaattcgat gaggcgccga aagaatcgat gatcaaaaaa ttctttcgaa gcttgtccga
      721 ttttacaacg atcatccttc ttgtagcagc ggtgatttcc ttttataccg catttgctac
      781 agaacatggg gatttatttg aaggactgct gatcatcgct atcgttgtga tcaactcggt
      841 attggcgatt gtccaagaag gaaacgcaga aaaagcactg gaatccttgc aagatatgaa
      901 taaacaaacg gcaactgtca tacgagacgg caaagtgacc actgttgagt cagagcaact
      961 ggtagtgggt gatattcttg ttttagagtc cggtgatgct atttctgccg atgcccgttt
     1021 gatcgaagca tcacaattac gtgtagaaga atcggcgttg actggagaaa gtgaagcagt
     1081 tgaaaaagat gcagcattta ttgctgaaga agatgagtca ttaggtgacc aacttaatat
     1141 ggtatttaaa ggatgtacgg ttgctgcagg tcgtgggaaa gcgatcgtca ctgccattgg
     1201 catggctaca gaaatgggta agatcgcgga tcttttgaac gaaaatacta tgcaaaaaac
     1261 acctttacag gtccgattga atcagctagg caaacgaatc agtatgatcg ctttagcggc
     1321 tgcggcttta gtatttgtga ttggtgaact gcaaggagaa ccactccttg agatgttcat
     1381 gacttcgatt tccttggctg ttgcagctgt accggaaacc ttgactgtta ttgttacgtt
     1441 gacacttgca tacggtgtgc agaagatggc gaggaaacat gcgatcatcc gacagttgcc
     1501 agcagttgaa acgctaggaa ccgctaatgt tatctgctca gacaaaacag gaacgctgac
     1561 acaaaatgaa atgcgcgtcc gccgggtatg gagcaaagaa gatgaagtaa cgaatatcga
     1621 agattcaatg acgaacagcg cgatggaaat attgaaaatg gctgcactat gtacagatgt
     1681 gacggtcgag caagaagatg acgaccttgt tatcaaaggt aatccaacag aagtagccat
     1741 cgtaagggct gttgaagaga attatcatac aaaggcagaa ttagaagaaa aatatccacg
     1801 agtaaacgag cttccttttg attctgaacg aaaaatgatg acaacagttc accaaatggg
     1861 cgaaaaatac atctcaatca ccaaaggagc attcgatgtt cttgctccgc gctttagttc
     1921 aggcgatgta gaacaagcgg cgatcgttaa tgatcgcttt ggtaaacggg cactgagagt
     1981 gattgctgtt ggttatgcta cttatgacga agaaccgcaa gatatttctt ctgaagcact
     2041 ggaaaaagac ttacgtttga ttggtctgat cggaatgatc gatccaccac gtccagaaag
     2101 taaaggagca atcaagcgag cgaaaaaggc tggcatcaaa acagtcatga tcactggaga
     2161 tcatgtggtg actgccagtg ccatagcgaa agaattaggt atcttgaatg atccgtcaga
     2221 agcactttca ggatcagaac tgcatcaatt atcagacgaa gaattagatg cgcgtgtcaa
     2281 agctctctct gtctatgctc gggtaacgcc agaagacaag atccggatcg tcaaatcttg
     2341 gcaacgtacg ggtgcagtcg tagcgatgac tggggatggt gtgaatgatg caccagcgtt
     2401 gaaagcaagt aacgttgggt gtgccatggg gatcacagga acagatgtgg cgcaaagtgc
     2461 cgcagatatg attttgacgg atgacaactt tgcgacgatc gtagacgctg tcagtcaagg
     2521 acgttcggtt tatcagaata ttcgtaaagc tatcaacttt ttgctaagct gtaatatttc
     2581 ggagatcttt attgttttga ttgcgatgct gttaggatgg ggagcaccat ttacagctgt
     2641 gcaactactg tttgtcaatg tagttgctga tggacttcca ggatttgctt taggaagaga
     2701 gccagcggaa cacggcatca tggatcagcc acccattccg aaaaatgaag ggatctttgc
     2761 tcgaggttta ttgcaaaaaa ttgccataaa tgcaggggtc ttcaccatcg taaccttgtt
     2821 tggttattat ttaggtagtt atgtcgatac tatcagccca tgggtagacg caagtcagca
     2881 tgtcggtcaa actgttgcat tcttgatctt agcttattca tcgattctgc atgtgttcaa
     2941 tgtacgcagc agccaatcga tcttcaaagt caatttagcg accaataaag ctttggtaga
     3001 aatggcgtta ctggcattag ctattacaac ggctgttgct ttgctgccat ttacgcaaga
     3061 actattcggc ctagtacaca tcagtttgaa tcactggttc ttggtaggga ttttatctat
     3121 cgtaccaatc gcggtgaatg aactgatcaa gttccatcga ttaccggaag cagaagaaga
     3181 ggaatgatta actgtttcta agttgatttt ctttttactg gaactataca aaaaggaggt
     3241 gtgacatgaa tgttgtcaca cctccttttt gctaatgggc tgaatgtttc ttttactgcc
     3301 tctcgataaa cggtgttctc ctgccacccc ctcggtcaca agtcacactc tcttttaaat
     3361 atcttttggc aattgttcat aaaatgctgc ttttgttgca gaaatgtatg ggctgagcca
     3421 tagataacca ataccagcag ttgctaatgc taagatatgc cagccgataa atgaaagatc
     3481 taaccagaaa agttgtcctt tataaccatc catcaatttt cgacttgcag tgatcgtatc
     3541 cagtacttta ggtgtttgtc ctgtttcttg gacgatatcg tagtaaataa aatagctttg
     3601 agaataagaa tagcttttaa tgattccagg aataactaat agcaaacccc ataatgttgt
     3661 aaagatcgag atcagcacag ctaagctgat cactccgccg ataaacaaac ctttgaatcc
     3721 acgaaatgca tctttgaatg gttcgatctg atctttttgt ccacgtaaaa tatcaagata
     3781 agtccaagaa ataccgctca tgaacaaagc gctaatgatg ctggaaataa ttccgccacc
     3841 gcctcctgaa ctaccgcctc ctgaagttgt cgcttctgag acgctcgctg atccatcata
     3901 tatggtggtg gcaaacaaga taataggaat cataataaga agaatagtag caataccaat
     3961 caatgtaggg attagattta gtaaaacagc ttgtccccaa cgtcctttta gactctcttt
     4021 tgcttggcgt tttaattctg cagaagattg catgattttt ccctcctttt ctttaataac
     4081 accaatatac catgtatcaa aaaatatttc tgtcataatg tttttctttt ttttgttatt
     4141 cataaaaaaa ggataaaaaa aagaatatta aataaagtag aaggaagccg agggaacttg
     4201 caaaaagcac ttgaaacgat tatgatggtg ggagttgtca agatatttta tatagaaagt
     4261 agggaaatga atgacgcaac ctgccattcg ctatcgttta atcaagaaag aaaaacatac
     4321 tggcgcgcgt ttaggggaat tgatcacgcc acacggaact ttccccacac caatgttcat
     4381 gccagtagga acattagcta cagtaaaaac aatgtcccca gaagagttga aagaaatggg
     4441 tgctggcgtt attttaagta atacatatca tttatggcta cgccctggtg aagacttagt
     4501 agaagaagct ggcggactgc ataaatttat gaactgggat caaccgattt taacagattc
     4561 aggcggcttt caagtttttt cattaagtga tatgcggaat atcgaagaag aaggcgtgca
     4621 tttcaaaaat cacttgaacg gttcgcgtat gtttttatca ccagaaaaag caatcaacat
     4681 ccaaaataaa ttgggctctg atatcatgat gagttttgac gaatgtccac cattcgatga
     4741 aagttacgac tacgtgaaaa aatcagtaga acgtacgtct cgatgggcag aacgaggatt
     4801 gaaagctcat gctaatccag accgtcaagg attatttgga atcatccaag gagcagggtt
     4861 tgaagatctt cgtcgtcaaa gcgcgaaaga tctagtcggg atggatttcc ctggttactc
     4921 aatcggcggg ttatctgtcg gggaacctaa atcagaaatg aatcgtgtat tagaattcac
     4981 tacaccgttg atccccgaaa acaaaccacg ttacttgatg ggtgtcggtg ctgctgattc
     5041 attgatcgat ggtgttatcc gtggagtaga catgtttgac tgtgtcttgc ctactcgaat
     5101 cgctcgaaat ggcacatgta tgacttctca aggccgtcta gtagtgaaaa atgcgaaata
     5161 tgcccatgat ttccgtccaa ttgatgagaa atgtgattgt tatacatgta aaaactatac
     5221 acgtgcctat atccgccatt tgatcaaatg tgatgaaaca tttgggattc gtttgacttc
     5281 ttaccacaat ttatacttct tgttgaatct aatgaaacaa gtacgtcaag caatcatgga
     5341 tgataatttg cttgaattta gagaagcctt ttttgaagaa tacgggttca ataaagaaaa
     5401 cgcaaaaagc ttctaaagga aaatctttta aaaattaagt aaaaaactag tgttatcagc
     5461 atttttctgc taaagtgttt tttgtagata cagaattagt gaggtgaaaa aaatgggaac
     5521 attaccaatg cttgtgatgt ttatcgcgct attagcaatg tggtttttta tgtcacgttc
     5581 gcaaaagaaa caacagcaag aacgtcaaaa cttgttagat agcatgaaac caggtgattc
     5641 agtcgtaacg atcggcggat tacatggagt tgtttcagaa atcgacaatg acaaacggac
     5701 agttgtttta gactgcgaag ggatcttcct tgagtacgat cgtgcagcaa tcaaaacggt
     5761 caaaccaggg actgtcgtaa caaatgatgc aacgactact gttgaaacaa cagaagaacc
     5821 agctgagaca gttgaagtaa aaactgaaac aacagaaacg ctggaagaga cagaagagaa
     5881 ggaagaaaaa taagtatcaa agcttattta aagacgaggt agggcagaaa gatgtcctac
     5941 ctcatttttt attcaaaaaa gaaagagata agagcatggg atatattgct caatattgaa
     6001 taatcgggtg tgtcatataa ataataataa ataactttat atatttttct atacaaacac
     6061 ctgtaaataa agcaatttta tatttaaaaa taactgtgaa aaaaatcaca aaaaagtgtt
     6121 tacatagaaa aataaatatt gtatgatata catgtgaatt atttaacaaa taaccatgac
     6181 ccattaggag gataatgatg gctaaagtaa tagaaaaaga aaaaacgaag acaattgacg
     6241 tacaagcaat gattgatgat ttggcagaaa aagcaaatgc agcattgaaa gaaatggaaa
     6301 gctttgatca agaaaaagta gaccatatcg tgcatgaaat ggcaatggca gctttggatc
     6361 aacatatgcc attagcgaag atggctgtag aggaaacagg ccgcggtgtt tacgaagata
     6421 aagcaatcaa aaatatgtat gcgtccgaat atatttggca taatatcaaa catgataaaa
     6481 cagttggtgt gatcaatgaa gatgtccaaa aagggttgat tgaaattgct gaaccagtcg
     6541 gtgtggtttg cggagtaact ccaacaacga atccgacttc cacaacgatt ttcaaatcaa
     6601 tgatcgcgtt gaaaacaaga aatccaatcg tttttgcttt ccatccaagt gcccaaaaat
     6661 cttctgcaga agcagcacga gtggtacgtg atgctgctat cgctgccgga gctcctgaaa
     6721 attgtatcca atggatcgaa catccttcga tcgaagcgac ttctatgcta atgaaccatc
     6781 caggtatcgc gatcgtctta gcgacaggtg gagctggaat ggtcaagtct gcttactcta
     6841 caggaaaacc agctttaggt gtaggtccag gtaacgtacc agcttatatt gaaaaaacag
     6901 caaaaattaa acgtgcagtg aacgatttga tcgtttcgaa aacatttgat aatggaatga
     6961 tttgtgcttc tgaacaagct gtgatcgtgg ataaagaaat ctatgcagca gtaaaagcag
     7021 aattccaagc acaccaagtt tatatcgtaa aaccagatga attgcaaaaa ttagaagatg
     7081 cagtgatgaa cgaaggaaaa tatgcggtca atccatcaat cgttggacat tctgcgatgg
     7141 aaatcgcaaa attggcagga atcagtgtac caaaaggaac gaaaatgctg attgctgaat
     7201 tggaaggcgt aggcccagat tatccgctat caagagaaaa actttctcca gttcttgcca
     7261 tgatcaaagc taacaacacg gaccatgcgt ttgatttgtg tgaaggtatg ttgaatcttg
     7321 gaggattagg acatacagca gttatccatt cagaaaacga agagttgcat gtcaaattcg
     7381 gtcttcgaat gaaagcttgt cgtattttag tgaatacacc atctgcagaa ggcggcatcg
     7441 gtgatatcta taacgaaatg attccgtcat tgactttggg atgcggttct tatggtaaga
     7501 actctgtttc tcgtaacgtc tctgctgtaa acttaatcaa tgtcaaaact gtagcgaaac
     7561 ggagaaataa catgcaatgg ttcaaattac cgccaaaaat cttctttgaa aagaactctc
     7621 tattgtactt agagaaaatg gaaaacgttg aacgtgtcat gatcgtttgt gaccctggta
     7681 tggtccaatt cggttactgc gatacagttc gtgaagtatt ggcacgacgt aaaaatgatg
     7741 tgaagatcga agtcttctca gatgttgaac cgaacccatc gacaaatacc gtatacgcag
     7801 gaacgaaact gatggcagac ttcaagccag atacagtaat cgctcttgga ggcggttctg
     7861 caatggacgc agctaaggga atgtggatgt tctatgaaca cccagacaca tctttctttg
     7921 gtgcaaaaca aaaattcttg gatatccgta aacggacata taagatcgac aaaccagaaa
     7981 aaacacaatt cgtatgtatc ccgacaacat cagggactgg ttctgaagta acgccatttg
     8041 cggtcatcac tgacagtgaa acacatgtga aatatccgtt agcagattat gcattgacgc
     8101 cagatgtcgc aatcgtcgat cctcaattcg tcatgtcagt tccagcatct gtcacagcag
     8161 ataccgggat ggatgtgtta acacatgcaa tcgaatccta tgtttcagtc atggcttcag
     8221 attatacacg tggattgagc ctacaagcaa tcaagttagt ctttgactat ttagaaaaat
     8281 cagtaaaaac acctgatatg gaatcacgtg aaaaaatgca caacgcttct acaatggctg
     8341 gtatggcgtt tgctaacgca ttcctaggta tttgtcactc agtcgcacat aaaatcggcg
     8401 gggaatacgg gatccctcat ggacggacaa atgcgattct tttaccgcat attatccgct
     8461 ataatgcaaa agatccacaa aaacatgcga tgttccctaa atatgactat ttccgtgcag
     8521 atacagatta cgcagatatc gcaaaattct taggtctgaa aggcgaaaca acagaggaat
     8581 tagtcgaagc tttagcaacg gctgtttatg aattaggaaa atctgtcggc atcaacatga
     8641 gtctaaaagc tcaaggagtc acacaagaaa cactagatac aaccgtagac cgtatggctg
     8701 aattagctta cgaagaccaa tgtacgacag ctaatccgaa agaaccattg atctctgaat
     8761 tgaaacagat catcattgat gcttacaacg gataagcaaa agaaagcgta gaaaaaagat
     8821 aagaaaatag atgaaaaaga acttggaaaa cgttgtttct ccaagttctt tttttaattt
     8881 ccaagtaact gaaagttact tttgttattt aacatttagg tcaattttag acagtttttg
     8941 ttcaaaaagc gatatttata gtggacggta ttactgctat attaaggata taaaatgaaa
     9001 gcgtttaaaa aggtggcgta aagatgatta ttaaagaaga gatttttgta cctaaagaat
     9061 tagtgaaaga aaaaattgac ctgctcgttg aaaacctgac aacgatcaaa gacgataatg
     9121 gtgaattttt gttggacttt gatggactga aagtagatga caaaagctgg acagtgtgga
     9181 attggccaca aggtgtcggt ctatatggta tttataaaaa ttaccagatg acaaaaaatc
     9241 ctcgcgctta tcaagtggtc aatgaatggt ttgaggatcg tatggaagaa ggcgctccgc
     9301 ccaaaaacgt caatacaatg gcaccgctat tgactatggc ttacttgtat gaagacacga
     9361 aagattcacg ctatttgcct tatttagaac aatgggcaga atgggtcatg aatgacatgc
     9421 ctcggacaaa tgaagacggg ttacagcatg ccacatatgg accggaaaat aagaatcagt
     9481 tgtgggacga tacattgatg atgacagtgc ttcctttggc aaaaatcggg aaactgttga
     9541 atcgcccaga atatttagaa gaagctcgtt accagtttat gatccatatc aaatacttga
     9601 tggataaacg aacaggattg tggtatcacg gctggacgtt tgaaggaaat cataactatg
     9661 ccgaagcatt ttgggctcgt ggaaactgct ggttgacgat tgctattcca gagatcattg
     9721 aaatcttaga attatcgaaa gaagatgcat tgcgcaaatt attgatcgaa acacttgaag
     9781 cacaagtgcg tgcattaaaa acttatcagt ctgaaagcgg tttatggcat acgttgttgg
     9841 atgatccttc ttcttacgta gaatcttctg caactgcagg atttgcttac ggtattttga
     9901 aagcggtcca taaacgttat ttaccacaag aatataaaga agtcgcttat cgtgctatcc
     9961 aaggattgct tgaacaaatc gatgaaaaag gagaagttca aaacgtttca atcgggacag
    10021 gaatggggga cagtttagat ttttatcgga atatcggaat cacagcaatg ccttatggac
    10081 agtcgttaac ggtgctttgt ttgacagaat tgctcgtctc ttattgttaa aagaaaggaa
    10141 acccatatga aaaaaacatc actgctgttt gcagcaagtt cgcttagcct aggtctgcta
    10201 ggtctaagcg gctgcggaaa taattctagt gtggcaactg tcgcagatgc agatgtaaat
    10261 gagatagtta ccttgcgctt tgcttatgca agcaacagcc agccagtaat tgatgcaatg
    10321 aatgaatttg ggcgtttagt aaaagaaaaa acagatggtt ccgtacagat ccagtatttc
    10381 ccagatggac aactgggtgg agaacgtgaa ctgatcgaat tgacacagtc aggagcagta
    10441 gatttcacaa aagtaagtgc ctcagcgttg gaaagtttct ctaaagatta ttcgattttt
    10501 tcagttcctt atctattcaa tgatgaagaa catttcttta aggtgatgga taatgaagaa
    10561 ataatggagc caatctacca atcgaccaaa aatttaggat ttaccggatt gacctattat
    10621 gattctggtc agcgcagctt ttatatggta gacggtccga tccatacgcc ggaagatttg
    10681 gagggaaaga agatccgtgt gatgcaaagc gaaaccgcta tcaaaatggt agagctttta
    10741 ggcggttcgc ccgtacctat gggaagtgat gaagtgtaca cgtctcttca atccaatttg
    10801 atcaacggat cagaaaacaa tgaatttgtc ttgtatacag ctggacatgg tggtgtggct
    10861 aaatactatt cctatgatga gcatacacgt gtgccagata tcatcatcat gaatgatgcc
    10921 atcaaagaac agctaactga tgagcaggaa aaagccattg aagaagcagc gaaagaatca
    10981 acggtttttg aaattgaagc ttttgcaaaa gcaattgaag aagaaaaagc tttagcacaa
    11041 gaagaacatg gagtccaatt caatgatgta gataatacac cgtttcgaga ggcagttgcc
    11101 ccacttcatg atatattcaa aaacgatcct acctacaaag acctttatca aaagatccaa
    11161 gagcaaggag cgtagctgtg atgaaaatga ttttaaacaa ggtattagag atcctgggag
    11221 ctgttttgct tgttgggatg gtcgctgttg ttttatggca agtattttcc agaacggttt
    11281 taggaaatcc aaatacggtc acggaagagc ttgttcgatt cggtcttgtc tggtttgcca
    11341 tgttgtcttc cgcgtatgtt gtgggacaaa aagcacattt agccgtaacg gttttaagtg
    11401 aaaaattaac tggcaaaaaa agtaaaattt tagaagttgt tgttcaaagt ttatttctgg
    11461 tatttgctgc gatgatcatg gtgtatggcg gatggaatgc tgtcacattg acaatggggc
    11521 agatctcgcc ttctttaagt attcctatgg gatatgttta tctgtcagta cctgtatcgg
    11581 gggtactcat cattatctat agcctgatca atctaatcga tacattaagc aacaagccag
    11641 caattcaaga aaaaatataa aaatggagga ataaaatggc aatagaagcc ggattagtgt
    11701 tattttttgt atttgttgtg ttgcttgtat tagggatgcc gattgctata agtattgcat
    11761 tctcatcgat gatgacttta ttgttagtta taccgtttga tgtttctgct tttagttcag
    11821 ctcaaaaaat ggtgggaagt atcaatagtt tttcgctgat cgctattccg tttttcgttc
    11881 tctcgggcat tatcatgaat aatggaggga tagctaagaa actagtggat tttgcgatgt
    11941 tgttcgtcgg gcgcgtgccg ggggctttgg ctcatacgaa tgtgttaggg aatgctttat
    12001 ttggttccat ctctagctcg gccattgccg cttctaccgc gattggcgga gtgttgatcc
    12061 ctcagcaagt ggaagaagga tatgaccgga agtttgcgac agcggtcaat atcgcgtcag
    12121 ctccgacagg tatggtcatt cctccaagta ctgcttttat catgtattca cttgttgctg
    12181 gtggtgcttc gatctctgct ttgtttatgg gaggttatct cgtaggcgct ttgtgggcgc
    12241 taggaatcat gctagtagca ttcatcgttg ctaaaaagaa taaataccct gttgtcgaaa
    12301 aaggacagat ggctcatgct ggaaaaataa taaaagaggc gattcctagc cttctattga
    12361 ttgtcatcat tatcggtggt atcttgacgg ggatatttac agcagtagaa gcttcagcga
    12421 tagctgtcat ttactccctg ttgattgcta tgttttacta taaaacggta aaactaaaag
    12481 atatacctaa tatgttgttt caatcagtag caatgtcagg gacgatcatg ttcttattgg
    12541 ctacttcttc catgatgtct ttcgccatgg cgtttaccgg tattcctcag gcaattagtt
    12601 cgttgattat gggcgtaacg gataataaat ttattattct gttattagtc aatcttgtgt
    12661 tattactggt gggtatgttt atggatgtgg cgcctgctat cttgattttt acaccaatat
    12721 ttttaccaat cgtcacaagt gtaggggttg atcctgtaca ttacgggttg ttttcaatca
    12781 tgaatctgtg tgtaggctcc atcacgccgc cagtaggtac aggactatat gtcggtgcaa
    12841 gtgtaggagg cgtaaaagca gaggcaatgc tgaaaccgct tcttcctttt tatggggtta
    12901 tcttagtcgt cttgtttttg attacttact tcccgcaaat cgtcatgtgg ctacccaata
    12961 tgctgagtta ggagaatagt tatgttgaac attacaatta gaggacatga tctttccgaa
    13021 gtccaaacga tagaagatct tgcagaaaaa acaaaagaac aagggattca tacactgcag
    13081 ctcgccttag gtctttcgtt tcctgaaaca ccttctggcg caaatgagat caattctggg
    13141 atgggaaatt atgtcaaacg agttttagaa aagcaagaag tgaccgtagg aattttaagt
    13201 tgctatatca atatgattca tccagattta acgatacgac aagagttgct gacaaagttc
    13261 gagcagtatc ttcgctatgc ttcttccttt ggtgcgtcga tggttgcttc ggaaactgga
    13321 tgtgttttgc cggaaatcca atatacggaa gaaaatttca cagatgaagc atttgctgaa
    13381 gctgtgtccg ttatccgtcg cttggtaaaa gctggagaaa aataccaaat gatggtgggg
    13441 atagaaccag gtttgaatca tccggtgtat tctttagcaa gggtagagca gctgatccaa
    13501 gcagtagatt cggactatct aggaatcatt ttagatccga ccaatttgat cacaagtagt
    13561 gattatcaag aacaagtcca attggtagaa gaagcttttg aacgttttgg tgaaaagata
    13621 tgtgcggttc atttgaaaga ttttcgtgtc gaacaggaaa aaatcgtgcc ggtaaatttg
    13681 ggagacggtg tgatcgagta cacaaaaatc aaagaaatca tcatgaagaa tcgtccgtac
    13741 ctgtatgtgg ttcttgaaga aacaaaagat gatgggattc gttatggaag aagtcttctt
    13801 gaataatgta aagaagacgt tggttaacta atatttagaa tggagggggc ggggctagaa
    13861 gatgccacgt ctcttctttt ttataagagt atataaaagc agctcttttt gattacaaaa
    13921 aacgtggtat aataaaattg tgcataagtg aacaaaagga ggtgcggcac atgctccaaa
    13981 agaaaatcag cggtgaacaa gtcaatacga tggtttctga tcagtatgaa atctatcatg
    14041 tcaaagatgt cgtcagccaa aataaaacga tctatcatca tcatgatttc tatgagatcc
    14101 atgctacatt aaagggagaa gcgacttttt atttgaacgg acgacaattt acgatcaaag
    14161 caggaagtgt gctgctcatc cattccaatg atttgcatcg tatcgtccgc caatcttcag
    14221 atgtattcga gcgtgtctat atctttgtca ccccttcttt tctagaaagt cgttccacgc
    14281 catggtctaa tctatcggct tgttttcaac caatgggaga gaagaggagt cgtgtattgc
    14341 aaatagaaca ggaagtattg aagcaaaaat tatcatttat cgaccatcct cctgataaaa
    14401 aaatttacgg tgaagacata aaatatgaac aagctctggt agaatacttg atttttctca
    14461 atcaagttgt acaaaaagaa gagagtgcgc aagaacagaa tgctgtagtt cctaatgagc
    14521 gtgtggaaca aatgttacaa tatatctcaa aaaatttatc tcatccgctt tctttagagg
    14581 aaatggaaaa gcagtttttt gtcagcaaat accatatcac acgagaattt aaaaagtaca
    14641 ctggatttac gtttcatcag tatgtaatga agaaaaagct gatctatgcc aagcacttgt
    14701 tgcgagaata ccgcagctct agtgctgtat atagtaaaag tggtttttct tcttatccgc
    14761 attttctgaa agcattcaag agagagttcg gcataacgcc taaagaattt ttgaaacgtg
    14821 atacacagaa acagttggtt cattatgaac attttgaaga tgaacaatga gaatgatcac
    14881 taaaaaataa ccgcagtagc tgctgttgtt tacatagaag cagaaactgc ggttatttta
    14941 tttttatcta tatatttcaa aatattttga aaaaatgacg tacatattat atgaatcagc
    15001 tgaatttacg aaagtctctc ggcgatagtc ctgtttttgc tttgaacagg cggctaaagt
    15061 aagcaaaatt ctcaatccct atttcttcgg aaatcattcg aaccgtaaat tctgtatacc
    15121 gaagaaggtc tttagcgtaa gaaagttttt tgctgatcaa ataatcgatt ggtggagaac
    15181 cgataaattt ggaaaaatct ttgctcagct gatatttatt caataagaat tgttgttcta
    15241 attgatcaag tgtaatcgtt tctttgaaat gttcgtctaa gtattgcttc agtttgtaaa
    15301 cgtaggcggg aatatcctct tcttcgaaat ctagctgata cttttgcaaa atcagttcat
    15361 tcagcaactc gtggatagct acagaggact ggaaatcggt acgggcattc gtgccttgtt
    15421 gcatgtggat caattctgtg atcaaatggt ggatcttgtt ttctgatgga tcccctgttg
    15481 aatggaaggt gttatggccg tcttttgtga actcctgata aaatgattgg acgttctgac
    15541 cattcaaatg gatccaatcc atttcccatg gttcttcgct gatcgtttga taacgctgat
    15601 attcttggca gtcaataaaa aataagtctc cagcttttag ctgttgctga ttatcattat
    15661 atgtaagcga acctttgcca gacaaagtaa actttatcaa gtaagaaggt aaatgtgctc
    15721 gttcagtata gtaagggatt ggtgctttga agtaaccgat ttcttggata taaaaaaata
    15781 aattcctgct tctttgtgta ggcgtcaaga tcactctttt tgagtcttcc gaccatgagt
    15841 ggttggcttt ttcccagtaa tccatgtaaa cactcccaac ttgtcaagat aatgcaatta
    15901 tataccaaaa tagtgtgaag caaaatataa gattctgtat tatgatagca ttgttaataa
    15961 atgataacgc tttattaata aagtgctaat caacaataaa gtaaaagagg tggagaaatg
    16021 aatccaacat ttgttgccat cgatatcggt gcatcaagcg gacgactaat gaaaagtacg
    16081 agggatgcaa aaggttcttt gatcttggaa gaagtacacc ggttcaaaaa cggctttcgg
    16141 aacaaagacg gtcacgatcg ctggaagatc gattatctta tcaaagagat tttgattgga
    16201 tttgaaaaat taaagcagac agggattgat cgcctttttg cggggatcga tacatgggca
    16261 gtcgattact gtctgattga tgaacaaggg gatcggataa atgatccgat tgcttatcgg
    16321 gacaagcgta ccaatggagc agtagaacag ttttctgaaa tctattctct agataagttg
    16381 tatgagcaga cagggattca gatccagcct ttcaatacct tgttccaatt gtttgctgaa
    16441 gaaaaagaaa atttggcaaa agcacataaa ttgttactga tacctgatta tttagggtat
    16501 gtctttactg gtaacatggt gacggagaaa actaatgcat ctactatgca gctgctgaat
    16561 gcagggacaa aagaatggga aatagaactt cttgaaaaaa tcggtgtctc gaaggaatta
    16621 tttccaccat tgacagagcc gggaacgata ctaggagatc tgcaaaaaga acaattttca
    16681 aaatatgatt tgccagatgt gacctttatc acagtagcaa gtcatgatac ggcgtcagct
    16741 gtattaggaa cgccaggtca aggagaacgt tggggttata tcagcagtgg gacatggtcg
    16801 cttttaggga ttgaaacaaa gatcgccaat gtttctcgca atgctttttc tgaaaattat
    16861 acgaatgaat ggggtgccca taacacggtg cgttttttga aaaacatcat ggggatgtgg
    16921 ttgatccagg aagttgcccg aatgcaagag tacgagtaca gttatgcgga attggcagag
    16981 cttgctgcaa aagaaaaacc gctgcagcaa gtgatggaaa tcaatgaccc tcgatttttg
    17041 aatcccccaa atatgataga agagattcag caatactgtt ttgaaacaga gcagccagtc
    17101 cctgtctcat cgggagctat cgctcgttgt gtgtatgata atcttgctct ttgttacgca
    17161 caagagctga tcaaagtaga acacttaact ggaacgaaat tggaaaagct gcacatcgta
    17221 gggggcggat cgaataatcg tctactgaat caattgacag cagattatgc cggaatcatt
    17281 gtagaagcag gacctagtga agccacagca atcgggaatg ttgccatgca aatgctcagt
    17341 acaggtcagt tttcgaatat agaacaagca agaaatgtaa tcaaacagtc gtttccatgt
    17401 gaaacttttt acccagatga aaataagcaa aacacgatgg aatggtacgt gaataaaatc
    17461 aaaggagtaa tcgtatgaac acgattgaaa gaaattacga acaagcaaaa gaaaaatatg
    17521 caactatcgg tgtagataca gatgctgtac tagaaaagat acaggacatc aaaatttcta
    17581 tgcattgctg gcaaggagat gatgtcaaag gtttcttgac accggatggc gaattgaccg
    17641 gtggaatcat ggcgacaggg aattttccag gagccgcacg aacacctgaa gagcttagac
    17701 aagatctgga gaaggcttat tcgttgatac cgggcaaaca caaattgaac cttcatgcta
    17761 tctatttgga tacagaagaa tctgtcgatc taaatgaaat tgagccgaaa cattttgaaa
    17821 aatgggtaga gtgggcaaaa aaagaagaaa tcggtcttga cttcaatccg actttctttt
    17881 cgcatccaat gatgaaagac ggctttacat tggctcatcc agataaagaa gttcgtgaat
    17941 actggatcga acacgggaaa agaagccgca aaatcgcgga gtactttggg aaagaaactg
    18001 ggcaaacaag tatcaataac ttttgggtac ctgatgggat gaaagataat ccgattgatc
    18061 gttttacccc acgggaacgt ttaatggaat cactcgatga aatttttaca gaagagttag
    18121 atgaacggta tgtacaagat gcagtagaaa gcaaactatt tggtttaggc gctgaagctt
    18181 atacagtcgg ttcccatgaa ttttatatgg gttacgggtt gactcgtgac aaacttgttt
    18241 gtctagatgc tggacatttt catccaacag aagtgatttc caataaatta tcttcattag
    18301 ctttattcag caaaggaatc atgttgcatg tttcccgtcc agtgcgttgg gattcagacc
    18361 atgtggtttt gatggatgat gaattgcagg aaatcgctaa agaattagtc cgtaacgaat
    18421 tgttggaaaa aacaaatatc ggtctggatt tctttgatgc aacgatcaac cgtatcgctg
    18481 cttgggtcat cggtacgaga aatacacaaa aagcactgct gaaagctatg ctggaaccag
    18541 ttgagcgatt gaaagaaatg gagctggctt ttgactttac ttcaagaatg gcttatacgg
    18601 aagaattgaa agatttcccg tatgccgatg tatggaatta tttctgttac aaaaatcaag
    18661 ttccagttgg tttggattgg ctggaagaag tccaggaata tcaaaaagac gtgttggaat
    18721 taagaaaata ggagtgtggc tatattgaaa aaaatagata ttttacaagc gccttatgtg
    18781 aaagaaatga tcgaaacgac gtcgaatctt tatcgcttgg gatgggatga aagaaatggc
    18841 gggaatattt cttatttact aaaagaagaa gagatcacac cgttcttgga tcctgaaaaa
    18901 gtgatccgcc gcatctcgat gatttttgat gccagccgtt tagcaggatg ctattttatt
    18961 gtaactggtt caggaagata ttttaaaaat gtagcgagcg aacctgccga gaatcttgga
    19021 atcgttcgag ttgctgaaga tggcaaaaca ctggaacttt tgtgggggct ggaaaacgaa
    19081 tgccttccta caagcgagtt gccaagtcat ttcatgagtc atatcgcccg tttagaagta
    19141 gacccagata atcggatcgt tatgcattgc catgctagtc atctattagc gatgagtttt
    19201 acgcatgaat tagacgaacg ttcctttagt cgtacgctat ggcaaatgtg tacggaatgc
    19261 ttagttgttt ttccagaagg aatttccatc attccgtgga tggttccggg aacaaatgag
    19321 attggggaag caacagctga aaaaatgaaa gaaactcgat tggttttatg gccacaacat
    19381 ggaatctacg ggtcaggaaa agacatggat gaagtctttg gtttgatcga gacagcagaa
    19441 aaggcagcag aagtctatac gtacgtcaaa gcgcaaggac caatcttgca aacaatcacg
    19501 gacgaaaacc tgtggcattt agcagatgcg tttggtgtca ctccaaaagc aggttatttg
    19561 gaggaagttc atacgaaagc aggtgtctga catggtgaaa aaagcttttt gcatgaaagt
    19621 ttacccagaa aaacaagaag aatacaaaaa aagacatgat gaactttggc cagaaatgcg
    19681 tacagcactt cgtgaacacg gggtcatcga ctattcgatc tatcttgatc cgagtacaag
    19741 tttgttattt ggttacttag tgatcgaaga tgaaaagtta tgggaaaaaa tggcaaatac
    19801 atctatcaat caaaaatggt ggaagtacat ggaagatatc atggagacaa atccagatgc
    19861 ctctccagtc agttgggaat tgatgccagt ctttgaactg tagcaacaag caactgggca
    19921 accggttgct tggggttctt tcgtttttat tgtgaaaaaa aatacaaatt aggaggaata
    19981 gaaaatgagt aatcgaatga ttttaaatga aacatcgtat tttggaaaag gagcgattga
    20041 gtcgatcgtc cctgaatttc agcagcgagg attcggcaaa gcagctgtca ttactgacaa
    20101 aggattgatc gagcacggta tagcaacaaa agtaacagaa cttctagata aagcagcaat
    20161 cgcttatgct ttatacgacg ggatcgtacc taatccaacg atccaaaatg taaaagatgg
    20221 agtggctttt gttaaagagg cagaggcaga ctgtttgatc gcaatcggtg gaggttcgcc
    20281 aatcgatacg gcaaaagcca ttgggattat tttgacgaat ccagagtttt cagacgtcat
    20341 tagtttagaa ggtgttgctg atacgaaaaa tccatgttta cctatattag ctataccaac
    20401 gacttctgga acagcagcag aagtaacgat caactacgtc atcacagatg aagtaaatca
    20461 ccgaaaattt gtttgtgttg atcctcatga tatcccaatc gtcgcgttca tcgacagcga
    20521 tatgatgatg ggaatgccga aaaaattggc tgcatcaaca ggtatggatg ctatgacgca
    20581 tgctattgaa gggtttatca caaaaggcgc ttgggaaatg actgatatgc tgcatttaaa
    20641 agcaatcgaa atcatcggcc attctttgga agtttctgta gatggcgatc aaaatgggcg
    20701 agaaaaaatg gcattaggac agtatattgc gggaatgggc ttctcaaatg tcggtttagg
    20761 actggtacac gggatggctc acccgttaag tgcatggtac aacattcctc atggcgtagc
    20821 gtgtgcagca cttttgccaa ctgtcatgaa atataacaaa gaatatactg gagaaaagta
    20881 ccgtgagatc gctcttgttc tagggattaa aggagcagct gaaatgtctt tagaagatgt
    20941 aagagaagcg gcttgtggtg agatcgatcg gttgagcaaa gcggttggaa tcccagaaac
    21001 catttctgaa ttaggtgtaa aagaagcaga tattccagca attgcagaag atgctttgcg
    21061 agatgtatgc acaccaggaa atccaagaga aacgacagta gaagaaatca ttgctttata
    21121 tcaaagtttg atgtaaaaac gaagcggatc ttcaacatta aattgcctgt aaagagaaat
    21181 ggtagtttat atctggatat ctcttttaaa ttgatggtat ttccgctata atttttctta
    21241 taaagaaata ggagaatatt atatggaaaa taaaaagcct tatggaactg tattgatcaa
    21301 agcctctaaa atactagact atttagcgga aaaccaagat gtctctttgc agacgatcgc
    21361 aaagggtgtg ggaatgacct cttctacagt cttgaaaata ctagatactt tgttgatgat
    21421 cggctacgta aataaaaatt cagagaaaaa ctatcgttta ggtgcaaagc ttattcgcta
    21481 cgcgaataaa aacattgaac aaatcgattt ggtagagttg actttaccgt ttttagaaca
    21541 gctgcagcaa aagatcgatg agactatcca cttaggggta ttggacaaca atgagatctt
    21601 gtacgtgaac aaactagatc caaaacatca aacgatccgc atgtcttcaa aaatcggaat
    21661 cacccgacca ttatacaatt ctgcgatggg caaagccgta ttagcagaat tcagtgaaga
    21721 gcagtacact gattatcttg aaaagcatcc gttgatccct tacacagaat atacgatcac
    21781 caatgcatta cggttgaaaa aggaaatcca aacggtaaaa gaaacccaca tcgcatttga
    21841 cgatgaagaa atcgaacgtg atattttttg tataggagcc tcaatcgtga aaaacgaaca
    21901 aatcattggt gcatttagtg taagtatgcc gaaatatcgt ttaacagatg agacaaaaga
    21961 acaaataatc agtgccctca agcaaacaaa aagcgaaatc gagcaaagaa ttcaaaaata
    22021 atgaaaaaag cgtttgcaat ttttcttttc tgccgtataa tatgggtata gatttccaag
    22081 ttataaaata aaatttctaa ataggaaata ggtggagaag atgaaaaaaa ttgatatttt
    22141 atctcgattg aaaaacgccg gagtgattgc ggtggtacgc ggaaaaagca aagaagaagc
    22201 tttgaatgct tgccacgcaa tcatcaaagg tggtctaact ggtattgagt tgacttttac
    22261 agtccctcaa gcagatcaag taatcaaaga acttttgtca ttctacaaag accaaccaga
    22321 aatagtgatt ggcgcaggca cagtattaga cgctgtcaca gctcgcttgg ctatcttagc
    22381 tggcgcagag tatattgtta gcccttcctt tgatcaagaa acggcagaga tgtgcaatct
    22441 gtatcaaatt ccttacttac caggatgtat gactattacc gagatcaaaa cagcgctgaa
    22501 aagcggggta gatatcgtca aactatttcc tggaagcgct tatggaccaa gtatcatttc
    22561 tgccttcaag gcgccgatgc ctcaagtcaa tatcatgcca actggcggtg tgagtctgga
    22621 caatatgaaa gagtggttcg atgctggtgt cgtaacagtc ggagtaggtg ggaacctttt
    22681 agcacccgca gctacaggag attttgacaa agtaacggaa gtcgcgcaac agtatgcagc
    22741 aaaaatgaaa gaaatcaaga ggtagctgct tatgggaaaa gtagtgacac tcggagaaat
    22801 catgttgcga ttgtccacag agcagggaac ccgtatcgat tcaagtgaac gattcgctgc
    22861 ccattatggc ggaggggaag caaacgtagc aatttcttta gctaattacg gacatgaagc
    22921 gatttttgct acaaaagtac ctgataacgc attgggaaaa gcagtcaaaa agcacttgca
    22981 atgttatgga gtagatacga aacaggtatt atctggtgga tcaagactag gtacttatta
    23041 tatggaaact ggtagtggcg aacgcgccgc ctcagtgatc tatgatcgtg ccggatcaag
    23101 ttttgctgaa atggatcatt tggaatggtc gctagaggag ttattccaag gaatagatat
    23161 atttcatgtt tcaggaatca ccccagcttt gtctaaaaaa tggcaaaaaa tgacgaaaca
    23221 ggtgatagaa gctgcaaaaa aagctggctg cctgatcagc ttcgatatca actatcgtgg
    23281 aaaattatgg acacagcaag aagccagcga agtaatccat cagcttttac ctttagtaga
    23341 tatctgctca gctggaaaaa tggatgctct ttatttgtta ggtatcgaga aagcgccaga
    23401 agcagaagaa caacctctaa tttattatta tcaaaaaatg caggaaaagt tcccgaatat
    23461 tcaggtgttt tattcgacca agcgtaccgt tcattcagca agtgaaaatg aattgaaggg
    23521 gacattgtgg atgaaccatg aatatgtgga atcccaagtc catagaatca ccccaatcgt
    23581 ggatcgagta ggtggcggag atgcttttgc aggtggcgtg ctccatggga ttttatccaa
    23641 tatgtctccg caagaaatca ttgattttgc tacggcagct tctgccttga agcatacgat
    23701 ccatggggac tgcaaccaat ttagtcaaga agaagtcgca caatttttag catgcggctc
    23761 tggaaaaatc attcgataga aggagtagaa gaaaatgcaa gaaatggaaa cacgttatac
    23821 acacagtcca gaagatatcc gtcattattc aacggaacaa ttgagaaaag aatttttagt
    23881 agaaaaggta tttgaaccag ggaaaatcag tttgacttat acgcacaacg atcgaatgat
    23941 ctttggtgga gtgacaccta ctgaaaaaga attagaaatc acattagaca aagaactagg
    24001 tgttgattac ttcttagagc gtcgtgaact aggtgtgatc aatatcggtg gaccaggatt
    24061 catcgagatc gatgggcaaa aagaagaaat gaaaaaacaa gacggatact atatcggaaa
    24121 agaaacacga catgttgtat tttcttctca agatgctgaa aacccagcta aattctatat
    24181 tagttctgtt ccagctcatc ataaatatcc aaatgtaaaa atcagtatcg accaagtaaa
    24241 accaatggag actggtgaag gcctgacact gaatgaacgt aaaatctacc aatacatcca
    24301 tccaaatgta tgcgaaagct gtcaattaca aatgggttat acgattttag aaccaggaag
    24361 cgcttggaat acgatgcctt gtcatacaca tgaacgtcgt atggaagctt atgtctactt
    24421 tgattatgcc aatgaagata cacgtgtgtt ccacatgatg ggcaaaccag atgaaacaaa
    24481 acatttagtt gtagacaatg aacaagccat tatttcgcca agctggtcga tccattcagg
    24541 tgtaggtaca agcaattact cattcatctg ggcaatgtgc ggagaaaata tcacctatac
    24601 agacatggat atggtgcaaa tggatcaatt aaaataagca taagggagag aaaatgatgg
    24661 aatttagtat ggatatgttt cgtttagatg gaaaagtagc attagtcacc ggtgctgtct
    24721 atggtattgg atttgagatt gcacgctctt tagctacagc aggagcaaaa atcgtattta
    24781 ataacttgaa tcaagaatca gttgatgaag gtatagcgca ttataaagaa gcaggcatcg
    24841 atgcaaaagg ttatgtctgc gatgtgaccg atgaagaagc ggttcaagcg atggttcaac
    24901 agatcaaaga agatgtaggt tcagttgata ttctagtcaa caatgcaggg atcatcaaac
    24961 ggacaccgat gatcgaaatg gatgcggcag acttccgcca agtaatcgac gtggatttaa
    25021 atgccccatt catcgtttca aaagcagtta ttccggatat gatcgaaaaa ggcggcggta
    25081 aaatcatcaa tatctgctcc atgatgagtg aattaggacg cgaaacggtc agtgcttatg
    25141 ctgcggcaaa aggcggatta aaaatgctga cgaaaaacat cgcttctgaa tacggtcaat
    25201 acaatatcca atgtaatgga attggaccag gttatattgc tacaccgcaa actgctccat
    25261 tacgcgaaac acaagaaaac ggtgaacgtc atccattcga tcaatttatc gttggacgta
    25321 cgccagcagc acgctggggc gagccaatcg atttggctgg accatctgtc ttcttggctt
    25381 ctagcgcatc tgatttcgtt aacggccatg tcttgtatgt ggacggaggc atacttgcct
    25441 atatcggaaa acaaccttaa aagagcttta gtacactgtt tagtggagag gttgtgaaat
    25501 cagcggattg aacgaatacc gaaggacagc tgattaaaca tcatttattc gaaaaaagag
    25561 gctgggacag aagc
//
LOCUS       DBNICI010000021        25531 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.021, whole genome shotgun sequence.
ACCESSION   DBNICI010000021 DBNICI010000000
VERSION     DBNICI010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 25531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.021"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(159..1469)
                     /locus_tag="AC1ZCC_000723"
     CDS             complement(159..1469)
                     /locus_tag="AC1ZCC_000723"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HIQ1628995.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2899)
                     /locus_tag="AC1ZCC_000724"
     CDS             complement(1481..2899)
                     /locus_tag="AC1ZCC_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIQ1628996.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3139..4707)
                     /locus_tag="AC1ZCC_000725"
     CDS             complement(3139..4707)
                     /locus_tag="AC1ZCC_000725"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HIQ1628997.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4711..5121)
                     /locus_tag="AC1ZCC_000726"
     CDS             complement(4711..5121)
                     /locus_tag="AC1ZCC_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HIQ1628998.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5118..6041)
                     /locus_tag="AC1ZCC_000727"
     CDS             complement(5118..6041)
                     /locus_tag="AC1ZCC_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HIQ1628999.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6038..6916)
                     /locus_tag="AC1ZCC_000728"
     CDS             complement(6038..6916)
                     /locus_tag="AC1ZCC_000728"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIQ1629000.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6903..8240)
                     /locus_tag="AC1ZCC_000729"
     CDS             complement(6903..8240)
                     /locus_tag="AC1ZCC_000729"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HIQ1629001.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8243..9346)
                     /locus_tag="AC1ZCC_000730"
     CDS             complement(8243..9346)
                     /locus_tag="AC1ZCC_000730"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88/105 protein"
                     /protein_id="HIQ1629002.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9420..10928)
                     /locus_tag="AC1ZCC_000731"
     CDS             complement(9420..10928)
                     /locus_tag="AC1ZCC_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIQ1629003.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10894..11586)
                     /locus_tag="AC1ZCC_000732"
     CDS             complement(10894..11586)
                     /locus_tag="AC1ZCC_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HIQ1629004.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11608..12582)
                     /locus_tag="AC1ZCC_000733"
     CDS             complement(11608..12582)
                     /locus_tag="AC1ZCC_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1629005.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12882..13754
                     /locus_tag="AC1ZCC_000734"
     CDS             12882..13754
                     /locus_tag="AC1ZCC_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1629006.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14212..16410)
                     /locus_tag="AC1ZCC_000735"
     CDS             complement(14212..16410)
                     /locus_tag="AC1ZCC_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1629007.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16642..17634
                     /locus_tag="AC1ZCC_000736"
     CDS             16642..17634
                     /locus_tag="AC1ZCC_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIQ1629008.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17694..19115
                     /locus_tag="AC1ZCC_000737"
     CDS             17694..19115
                     /locus_tag="AC1ZCC_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HIQ1629009.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19108..20607
                     /locus_tag="AC1ZCC_000738"
     CDS             19108..20607
                     /locus_tag="AC1ZCC_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HIQ1629010.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20646..22040
                     /gene="uxaC"
                     /locus_tag="AC1ZCC_000739"
     CDS             20646..22040
                     /gene="uxaC"
                     /locus_tag="AC1ZCC_000739"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HIQ1629011.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22199..22882
                     /locus_tag="AC1ZCC_000740"
     CDS             22199..22882
                     /locus_tag="AC1ZCC_000740"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HIQ1629012.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22884..23720
                     /locus_tag="AC1ZCC_000741"
     CDS             22884..23720
                     /locus_tag="AC1ZCC_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIQ1629013.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23731..25485
                     /locus_tag="AC1ZCC_000742"
     CDS             23731..25485
                     /locus_tag="AC1ZCC_000742"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1629014.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
BASE COUNT         7789 a         5195 c         4478 g         8069 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa
     2881 cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt
     2941 ataactcttt ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga
     3001 cagataaaaa acaaaaaata atctaaaaac attgatgcat aagattgata acttttttta
     3061 cataacaata tatacaaaca aaaaaattca ctaactagtg aaaataagtt agtgaacctc
     3121 atttattaaa gaattaaatt acagatgatc ttttccatca tacttgaaaa actcaaaatc
     3181 agcataactt cctctttttt tatttagatc atgcactgct atcccgataa aatttccagt
     3241 aaaccctcct gagagtatac acatgttttt ttgcggacct actttcttcc attcttcagt
     3301 tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt acagtgactt tcaaaaaagt
     3361 cttcttttct gtgagtggaa tcgtccaatc ttctatttgt gctactccgt tttctgattc
     3421 tatcatacgt aggcattttc ccttgtcctc attccaagta acataacaat aaatataatt
     3481 ggattcattt aagtacagta ccagtccagc catctgatta aaattattag gcatgtattc
     3541 aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga atagctaaca aatgatgttc
     3601 gaaggtagat tgaagagatt caccggaaat cagacgtaaa aaacctggtc tacattttgt
     3661 atcacaccaa tcagcatttg gcaaaatgcg tctggcattc cagttcttct ttagctctcc
     3721 ttcaaatgta tccataaact gatgatcttt ttcttgttgt acctcttcat ttgtctcaat
     3781 cattatttct tccatcggag ttgtctggct atctttaagt cttaaccaat cgtcggacgt
     3841 ccaatacact tcttgaatag cggtttctct tcccaaaata gccgcaccgt ttaatggtct
     3901 ggtacaaaga tatgtcatgt accatcttcc tgacggggtt tgtactaagc tcccatgtcc
     3961 tgtacattgc aatgtagaag caggtttgtc actagctgtc agcataggaa aaccaggatc
     4021 aagctcgtaa ggaccagtaa tattctttgc tcggcaaact gtaactgaat gtcctcttcc
     4081 tgtaccgcct tctgccgtca tcaaataata atacccgtta cgaaaataca aatgtggtgc
     4141 ctccgttttc gctaattctg tacctgcaaa tattttatag atgggaccat caagcttctt
     4201 cttctctgca tcatattctt gtaatacgat accgctggat ttattcggtg tgtccagacg
     4261 ataatcccat agttcattga caagccattt ttttcccgtt tcaagatcat ggaacaaaga
     4321 aggatcaaag ccgttactat ttaaataaat tggttcggac caaggacctg tgatctcaga
     4381 tgcagtgatc aaataattgt ggacatcttt aaacggacgg cctgcacttt tgacatctgt
     4441 gtaaacaaga taaaacaatc catcagcata actgatttgt ggcgcccata tacttccatt
     4501 tgtaggattt ccttgcaaag atacttgagt ggtcaaaata tccgttgtat gtgaccagtt
     4561 gactaaattt ttcgactcat atactcggat tcctggaagc cattcaaaag aggagacaat
     4621 aatgtaatac cgctctttca ctcttaatat attaggatct ggattgaatc ctcttaaaat
     4681 aggatttttc actttgatag tctgcatgat tcaggctcct tttctcattc ttactcgata
     4741 taaactgaat gccccagctg cgaaataaaa aaatgcaaaa ggaaacgcaa taaactgagt
     4801 gccaatcaac aatatgaatc ctgttataaa atacaaacca gtggcagttg ttaagtattt
     4861 tcctttagca gtcaaaaaca atgctactgc taaaaaagca agtgaaataa acaacacaac
     4921 ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta ttttctaaag acaaagttga
     4981 atgatctaca gcaagcgact gataactttt tttaaactct gacgcagaca tattacttaa
     5041 agtaactgct aacccgccaa agaaaatcaa agatagactg ccaccgacca ttcctagcaa
     5101 tagttcccat tttcttttca tttctgaacc ccctgctttt ctaaatgtat ccattcaggt
     5161 cttttgatcg atgtttcata attgttatta cgttcagaga atttgacagt ctgttcattt
     5221 ttcttgtcat cccaattatc ccaaccagtt gggtgaacat gcccacctat cttacacgct
     5281 tcaaataaga catttgcata cggacgccac ggtcttccta aatagacgtt tctagtatca
     5341 ggttctgcag tcaaggtaca gctttcaaaa cgataccctg ttttttgatt tttcggcgta
     5401 gatgctgctg tgatgtatcc aggattttta ctggctaagc tatggatatg acactgatga
     5461 aaaacggcat ctgccccacc aaatatgaaa tccaccgttc cttcaatcca gcaatattca
     5521 taggtctgac gacaatactc aggattggca gaagccggtg tacaaaaagg agtaccatct
     5581 ttttgcaaat caggcaacgg acccgtgcat aaggtatcct gataaccaga tagtcgacag
     5641 ttttttaaag atacgcgatc tcctgaattg aataaagcaa cagcctgtcc taccttttct
     5701 cctggacctg ctgtattttc gatgtgaaga ttttctaata cgacattcga tccttctaag
     5761 aaaaaagtag cacttcggaa agtccctaac tggctaccgt caagatactc ctgttttgca
     5821 aaacgattac cggagataac tactgttcct gctcccacaa gtttaaaatt ggataatctg
     5881 ctttctatat attcatcata tcttccttct tttactatca agacctttgg atggctatcc
     5941 ggcaggcctg ccaaataatc gattccttct tggatagttt caaaatccgt cggggtatct
     6001 atagcattct ttccaattat cagtttgtct ttcatcctca taaatgctcc cttatgaatt
     6061 gtttccaaat atttctcatt tcttgtgttt ccatatgacc gcctgttaaa gaaaaagaag
     6121 aaaagttttc tggactaccc attgattgat actttttgtc cagccctttt ctcagcttca
     6181 gtacgccttc gtccaagcac atcgtatcgt tttttccaac taagctaaga cgagggcgcg
     6241 gagcgatttt ttcttggatt gctaacgttg tataagctgt caatagattt ggcacatagt
     6301 aataaaaccc atgatgatct aggcggcgat gtagcaaaag tatttcgatg tccacctgtg
     6361 ccgctagatc aatacaaact ttgacacgag aatccaatgc tgtcacccac cagcttaata
     6421 agcctcccat agaaagacca atcgttgcga gccgttgagt atctgtgtct tcccttgtct
     6481 ccaaataatc gatcaagctg atcacatcat aaatacgcat tccccataac gtttttcctg
     6541 ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc tccgcgttct tcaaatcccc
     6601 aagcatcaat cgaccaaaca gcatatccca ttcctgtcag tacttctgca aaagatggag
     6661 aagctaagta aggagcacca tggatcaatt cacttttccc gactgaaaaa tctccgccgt
     6721 gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt ttctggataa gcatagaggg
     6781 ctggtactgt ttccaattca tttaagtaca attgatagta ttcaagcata aaaccgtttt
     6841 gtttttctcg gtataatcgt tttccactag gggtatgtct gctagatgga gcacctaaaa
     6901 aatcattcaa ctgcataagc aaagttttta cgaacaacca gttcatccat attttctgat
     6961 tgatttgatt ggaatttctc aatactgatt tctttagtat tttctaatag aaatgctggt
     7021 ccctcgtgat tttcaatcga catctggtta aaggaaacct tacccgcaaa gccaatataa
     7081 aatccttgtc tgctcatttc ctctattcct gtcatcatag caggaacacc gggaactgca
     7141 tcttgggcca tcgagacatc gatattgtca aatgtgattt cttgtatgaa ttgttccgct
     7201 aatccataaa taaaacctgc agaagcatgg acattccttg ccgtgatatt cgaaaaatgg
     7261 atccttctga atgctggtgt ccgttcatct atcggataag cctttttttc ccatacatac
     7321 ggttctttcc ctcgcggacc acagaaataa taaagattca gtataaatgg acacatcaca
     7381 ttatccatga ctatattgga tactcgaata tcttcaacga tcccgcctcg acctcgtcta
     7441 gatttcaatc gtattcctcg atcagtttct tggaagatac aatttgaaat ggtaatatta
     7501 cgaatactac cactcatttc acttcctaat acgacaccgc catgtccatg gaccatcgtg
     7561 caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc agtccctgcc
     7621 ttgattgcaa tacagtcatc tcccacgtca atatggcaat tactaatacg cacatttttg
     7681 catgattctg gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa gatcgtcaag
     7741 ttatcaaacg ttgcattact gcaaagaatc gggttgatcg tccaacttgg tgactggatc
     7801 aatttaatat ctttcacagt aattcgatga cagttatgga aactcatcaa tttaggacga
     7861 ggataagcga gattatctgg ttcatttcta aaagtatgcc accatttttt tccatttcca
     7921 tctaatgtac cgaaaccagt gacagagatg ttctctacat tttgagcata gatacaggaa
     7981 gcataaactt ttcgatggac tccttcccaa cgtgaatgaa caacaggata atcttttgga
     8041 tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa gttcgacatt gctttttaaa
     8101 aataaagctc ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc tccgtcactt
     8161 gctgcagcat caatggcttg ttgtattgct tcagtattca attcatcaat tgatgcgcca
     8221 aatgtcaaaa tatcatacat atttataatg cctccacttc aatcaatgct tgcaagaatg
     8281 caccaacgcc tttttgatca ttacaaataa ttggttcact gatgtagtaa gcatatgaac
     8341 catctcgctg atctgctccg cctaaccctg ctacttgaca gtttttattc aagttcagcc
     8401 agccttcttt tgtttccaga acaaattcat caagtaatcc ttgatgtgat ttcttcactt
     8461 gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc attgcgtaaa
     8521 caaacatact actgccggaa gcttccagat aatttccttt ccgttttgtt tctgttgtta
     8581 cttgatacca tgtcccgctg ttttcttctt gatatggaag taatgcagcc agtgtctttt
     8641 ctagaatttc tttcagcaaa gaacaatctg tttcttcatg aagtaattca taggtatctg
     8701 ctgcagccat caaataccag ccaatcgatc ggctccaaaa atgatgggat agacctgttt
     8761 ttggatcagc ccatggttgg accttctttt catcccatgc atgaatcagc aaatttgttt
     8821 ttggatcgaa tagatgttta taactgattt caaactgtct caaaacatct tggtaatctt
     8881 tttcttcact aaaattctgg ataaattcag cataaaaagg agaacccata tataagccat
     8941 ccaaccaaat ttgataagga taaatttctt tatgccaaaa agctccttcg gatgtgcgtg
     9001 gatggtttgc caactgctgg gaaaggagtt ctgctgcttt tttatatttt tcttcgcctg
     9061 tttctttgta caataaaaag aaaagctttc ctgtatttac atggtcgata ttgtattctt
     9121 caacgctgta gccacggatc gttccatctt cttgcacgaa atagtcgata ttgtctttta
     9181 tgaaatcaaa ataaattttt tcttgggttc gttcccaaag taattggaac cctttaagta
     9241 cgacgccata gtcataagac cattttcctt tataccactt ttcttcatat aagcgaggtg
     9301 ttctttccat gatacttttt gctaatttct ttgaccagtt ttccattatt tgctctcctt
     9361 taaaaattaa aataggattt gggacaaata ccaagaagta tttgtcccaa tcgtctatat
     9421 tactgtgccg ctgctttgta tgcttcctcg tactcttgtg tgattcgatc gcctccttgt
     9481 gctttccagt cttctacagc ttttttgaat ccatcttcgt caagctgtcc catgatgtat
     9541 tgcaccgtag catcaatgac gattttttca atttctgatc ctacttcatt gttcgtcggt
     9601 gaatccaatg gcacagaagg atccattaca gcaaattctt cattttcttt gatcaattga
     9661 ttagagaatt ctttttcagg gtctgtatct ttgaaagaga acgtcacttc actaggacga
     9721 gagcttgata acggttgcac ctcttgttgc caaagatcca tatcgataaa ttcgatagaa
     9781 ccatcatcat tcaattcata atgcgttcct tcgataccgc cagtcatcaa tttatacaca
     9841 tcttcttcta aaagatcatc aacaaattga agtaatttct tcaattgtgc ttcgtctttt
     9901 acttgagatt tgggaaatgc tagcaagccg ccaataccat ttccttctga ccaaacatga
     9961 taaccgccgc cttcaccatc tgagatcttg tttacaggca ctaattccat atcatcttga
    10021 ataccttgac ccatcgtctt cagattcttg atatccacca ttccagtgta gattccagtt
    10081 cttccttgtg caaattgttc ttgctgatca gtcttttggg taactgcaaa gtcttgtgcc
    10141 aaatagccat tctcaaatag atctcttgaa aaattcaaag ctttcatata ctcttctgta
    10201 tcaaattctg gagtgaattt gccattatcg tctacagccc agccatttgg tgctccaaaa
    10261 taagaagtga aaagtttaaa gctggtataa cgaatatcac taggcgcacg atcgccaaaa
    10321 ccaactgtgt cgtccacacc atttccatca ggatcgtttt ctgtaaattg tttagctact
    10381 tcataaagct catccatggt agttggaaca tctagtccta gattatccaa ccagtctttt
    10441 cgaatgacta agccagcacg agcgtagtct ttttgtatcg gtacgccgta caaggcaccg
    10501 tcgatactcg cagcttcgat tctatcttca gaaatttgac ttaaattttt atagtctttt
    10561 aaatattctg atacatccca gaatacgcct gattttaacg cattgcggac agaagagttt
    10621 ttcaacatcg caatttgaag tgtaacgata tctcctagtt catcagaagc caatgcagtt
    10681 gttaatcttt cttcttttga agcatcagga atccagttga atgtcagttc cgtatcggtt
    10741 ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg caaaatattt
    10801 aaccaagtga ttttagcttt tccatcttct gtctctgaag aactgttact tgaactgctg
    10861 ccgcatgcgg caagtgctag aattcctgca aaactaagac ctaattttac gagcgttgct
    10921 tttttcataa attttctcct tcactgtttt aataatattt ttgttttctt ctgttttttc
    10981 tgtatatgag ttcttccatt ccaaatggca aaatatagaa tttgctgtcc acgtgactgc
    11041 ggaaaagaag agactgccac cgataaaggt taaaacacct ggcattgttt ggatgatcat
    11101 cagataaatc cctaccatac ttaatatcaa cactaatgag taatgaagat gtgagaatcc
    11161 gaataaaaat gccattttca ttttgaaaaa atatcctttc caatcataat gagccatgat
    11221 tgggaaaata tagagtgctg agagcacata tatgaacgca gcaatataca aagcgatcct
    11281 aagataccaa ttgtgtatga aagaaagatc cacaagtaaa acaatcccga tggcgccata
    11341 tatccagctc atcagcaagg tttcacgata gttttcttta aaatatgtga agtacgtttt
    11401 cgtcaaagga aacgtttctt ttgagcgcag ccattttcta atcactgcaa ccatcgcata
    11461 cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc cccacagaaa
    11521 attcaaataa accaactgta aaatacgatt tagccttccg tttattttat ctaatgactg
    11581 cagcatatct ttccctctct ttctatctta gtaaacgccg tcttctccta gctttttggc
    11641 aatcttatta gctaaaatta ccataaacaa tcctacgacc cctttaaaca atcccattgc
    11701 tgtactgaaa cttaactgtc cattcttcaa tccagctgta taaatatagg tgtcgaaaat
    11761 ttctgcaacg cttcgattca atgaattcaa taacaagtac atatgctcga accctaagtc
    11821 taatgtctga ccgattttta gaatcagcaa ggtaatgatt actggacgaa ttgccggcaa
    11881 tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc atttctgcag cttcatacaa
    11941 ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc gtagaccagc ccaactcttt
    12001 ccagatcact tgtccgatat atactgtgcg tgtccattca ggggatgtta gaaaactgat
    12061 tttttcaaat cccattgttg caaggaattc attcaatacc cctccgtcaa cattcaaaaa
    12121 tacataagta atcgaaacaa tgattaccca agacatgaag tgagggatgt aaatgatcgt
    12181 ttgaatcccg tttttcaatc gtttgttcgt tacttcattt aatagtaaag acaaaatgat
    12241 tggcatcgga aaaaacacga agatatttaa tccgaacaag atcaacgtat ttcttaataa
    12301 tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga cccaaggact
    12361 tcccattatt cctaaaaatg gttgataatc ttgaaaagca ataacgagtc cccccatagg
    12421 aagataccta aaaatcaaga aataaaggac tcccggcaga atcatcacat ataaaaattt
    12481 attcgttttg attcctgcaa ttattttttt tcgtcttctg agcttttctt cttttttcga
    12541 tagcgtcagt tgaaatggtt cttgtttctt tacactttcc ataagcccct ccttagtttt
    12601 gtgaatgttt gctgcttatg acttcatgat agataactat ctgaattacg tatataatca
    12661 tttttaccgt aagtaaaaac gcttacatag caatgatttt tgtgctatac aaaacgataa
    12721 agtgtatttt ttcacaaata aactacgcat agcaaacctt ctattttttt ctatctgaca
    12781 cttagcatag gaaatgatta tatacgtaat ctttcaccga tgataaattg aacatataga
    12841 aacagaaaca tttaatttat tttgaggagg agcctatcta tatgaaaaag aaagtctcta
    12901 taggagaaag aagttttaca cttttcaaca atacgtttct aattttgcta gctttgatct
    12961 gtatcgttcc ttttttaaat atcatcgcta catcttttgc ttctacacaa gaagtcgtcg
    13021 cgaaaaagtt tatccttttt cctactacct tttctttaga cgcgtatcgc tacattcttt
    13081 ctacgccgac catttttaga gcacttgctg tttcgattgg tgttacaggt gtcggaacga
    13141 tcgtcagtat gtgtgcaact tcactgatgg cctacggtct ttccagaaag tatttgtttg
    13201 gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt gttcagcggg gggatgattc
    13261 caacgttctt agttgttcgt tctctagggc tggtcaattc ttactgggcc atgatcctgc
    13321 ctgtggctgt taatgcaatg aatatgatca ttatgagaaa ctttttccaa gccttgccgg
    13381 atagtttgga agaatcagca aaaatggatg gttgcacaga ttttggtgta tttttcaaaa
    13441 ttatgctgcc attagcgtta ccgtctattg ctactatctc gcttttttat gctgttactt
    13501 actggaacac ttatatgact gctattttgt atatcaacga ttcatctaaa tggccgatac
    13561 agattctttt acgccaaatc gtcattgtat caagtgggat gcaagcagaa agtagtgccg
    13621 ttgatgtgat tccaccagca caaacagtta aaatggctgt tatcgttatt gcaacagtgc
    13681 ctatgctgat tgtttatcct tttgttcaga aatattttgt caaaggagca ctcgtaggtt
    13741 ctgtcaaagg gtgattatgt actgaacgca aaatggtagt tgtgtcagaa gtggtcagct
    13801 tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa atttcggctt
    13861 atttttcgaa gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa gagacgttca
    13921 gccttgagcg atgataaaaa ctctcacaaa cattgcgacg agtaacccac aggagcaata
    13981 agaagcgatt ttgaaaaata gcttcccata tttcacaaaa ttatgcctta ttgtcgatag
    14041 cctgtctctt gaacgccatt tattcgtaaa aaagaactgt gacaagactt ttgtcacagt
    14101 tctttttgcg tgcttagcct tgaaatttaa ctatccataa atacaaatac tagggttatt
    14161 ctaacttctt ttattgcttg aatcagtcca caagaaaaac agcctcttga ttcacaaata
    14221 tttttttcga tattctcccg gcgtaatcga ttccttcttt ttaaagaaac gaataaaatt
    14281 ttgcggattt cgatattgca atctttctgc aatctctttt acggagaggt tagtctcttt
    14341 caaccatttt tttgctacct ctaaacgatg attttgaaca aaatcaccaa atttttctcc
    14401 tgtttccttt ttaaatacat tacttaaata gttagggtta taatgcaacc tatcagcgat
    14461 cacatctaaa gacagatcac gatcatattc tgtgtagacg atatgcataa tattctccga
    14521 caaattttta aattcatgat cagtgattgc ttgagagcat tctgtgattg gcagaactaa
    14581 ttgttctaaa acaagttttt ctagttcttt gggatgataa ttgttcagtg cttttacata
    14641 aatttgtttc atgttatcaa agttttttga atctactccc aataactgtc ctaattgaac
    14701 gagttcattg atcaagcgga ccgaaactac ttcccgactc aaaggatttt tattttgtgc
    14761 aaacaatgca tcaatcaatt catgtactaa attcttcact tcctgatttc cagaacgaat
    14821 ggcttcaaac aatcgatttt gttcttctat cggatatcta ataagtgttt tttcatgagc
    14881 agcaggaacg atttcctgat aaaaaataat tgaacttgga ccagtattta cacgataata
    14941 cagggcttct tttgcccgat caaccgattg cttgctttca ttcagcgttt cgaaccggtt
    15001 actgatgcct atactgatag ttaaattcaa gtattctttt acacgttttt gaatgagtcg
    15061 gcaatactcc atgacttttt tgcttgcttc tgcatcatct ttatccattc gataaatagt
    15121 ggcttgcatt tcttcattca aaacgatcgg cagcattcga ttttcctttg gcacaatttc
    15181 cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc tccccaccgt gactatcttc
    15241 taacagatcg atttgaacca aagctgtata atagcagtca ttttgagaac tataaccaaa
    15301 ttgctgcaac cgctgatcta gttctcgcct ttctactcga tttctaaata aactaagtac
    15361 aaatagcgtt tccagctgtg gcttttgggt aactaagtgt gcacttaacg cttctttttc
    15421 tcctatgatc cgactgactg attgagcgac caaatctagt tctttcccac tgaatccttt
    15481 tgatttaaga ttcagacgtt cctgtatttg agaaatcgga cgggcaaatc tgtccgaaaa
    15541 taagtaggaa agaagaccaa tgagtcctac cagtgttaaa gacactgcaa ctagcccgat
    15601 tctcagattg cggatcattg cgccaattgc actagagtca acttctaatc tgtaaatcca
    15661 attattataa tctgatttca gtactatata cgttttatct ttttctctta tcactttcag
    15721 ctgattgtaa tctagatcca cgtccgttaa tacagttggt tgctctttat tttttactgt
    15781 ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa agtatgtctt ctccactttc
    15841 accaacaact tgttctattg tatgtttctc tatcgtagca attcccaatg catatttttc
    15901 tttttcaaac attggcaaag aaatcaccat ttcgattcct ttgtttttct tttgccaaaa
    15961 taaattattt ttgttattta tgtacttctg ttgatacttt tcaacttcct cttcggtcag
    16021 ttgactcaac gatccattga ttactcccca tttaccagcc aaactgacta aatcataatt
    16081 attatcatcc ataacaatcg tttctataaa atttagttct ttcctgattt cagaataagt
    16141 ggagtaatct gtatatgtca gcggatgctt gaatatgtcc gagaaatttt tggtcgaacc
    16201 atacgtagaa aacgaatatt caatcgttct aattttccat tcaacgttag ttttaacttg
    16261 cctgagcaaa ttctttttgt ctgttaaata actcgattcg atcgattcat aggtattctg
    16321 gtaaatgaat aaactaaatc ctatgacaag tacggctcct aataaaaaca tcggtaaaaa
    16381 tattttataa aatactgtac ttttcttcat tttcttcctc tcctcctttt aaaataaaag
    16441 gctttcattt ttctgtaaaa ttgatactca tctaattaaa tgagcattcc ccgctttcaa
    16501 gaatagcata attaaaaata aaaatccaag taatttttaa cgtgtgcaat cttttcatat
    16561 caaagatcta ttgacttatc gtagttatcg taatatgatg aatgcgttaa caaaaagtga
    16621 aaaagtaagg aaaatgatgg aatggtaacg atcaaagata tcgctaaagc agctggcgtt
    16681 tctcatacga cagtttctcg tgctttgaat aacagcagtt taataaaaaa tgaaacaaaa
    16741 caaaaaatac gacaactagc taaggaaatg aattattcgc caaactatag tgcaaaaggt
    16801 ttagtgaatc aaaaaaaata tctaatcgga ctatttttct caagtttgca taaaggaact
    16861 tcatcaaatt tcctagcaga ttccattgca ggcatccatt cagtattaga cacaaattac
    16921 agtttatctg ttgaaagtat cgatgttgtt gatttaacag aaatcaacct gcaacgctac
    16981 gatggactga tcgttatgag tcaaagtgat gatgatcagc ctttcatcga ctatctcaaa
    17041 gaaaaagaat ttcctttcgt tgttgtcaat cgtcacatca atgatccagc ggttatcaac
    17101 gtcactgccg atgatgcggc cggtgttatg caggcagtag attacggtat tggatgcgga
    17161 catacgaaga tcgcttatat tggcggaaag aaaaattttc gttcgacaaa agaacgggaa
    17221 aaaggggtat tgaaaagtct aaaaaaagcg agcatccctg tcaatccagc ctatttttta
    17281 acaggagatt acagcttaga aagcggcctc caaaacatgg aatacctatt atcactttct
    17341 gacctgccta ctctggtttt ttgcggcaac gacgacatag cgattggtgc attgagagct
    17401 gtacatttag ctggcctccg tgttccccag aacatttctc tgatcgggtt cgatgattct
    17461 ccagtcgtct cctatctcaa tccgccgctt accaccgtac gtaagccttt gaaagaaatg
    17521 tgtcaagaag gaacaaagct tttactagat ttgatcgaag gcaggacgat cgaacaatct
    17581 aaaattgagc tctccacctt gttgatcctt cgtgagtcga tcaattactt gtaaactaca
    17641 agtaatttta tataaaaatg ttaacgtgtg cataacacag aaaggaagtt tatatgaaaa
    17701 aactgacaaa agaattagtg aaaaaaaacg aagcacctgt aaaagttctt cagtttggtg
    17761 aaggcaattt tatgcgtgga tttattgatt ggcagattca gcagctgaat aaccaacaat
    17821 tgtttaaagg taatgttgca atcattcaac ctttagaaca aggtcttgga aatatgatga
    17881 accagcaaga taatttatat acggtcattt tacaaggact cagcaatggc gaaatcgttg
    17941 atacttccga gattatcacc gtagtagaac aaatgatcaa tccttatgat gaatgggatg
    18001 cttatctatc tctagcagaa aatgacgact tggcctatat cgtctccaat acaacagagg
    18061 caggaatcca attcgtggag actgataatt taacagatac tcccccacag agcttcccgg
    18121 gcaaactgac agccttttta taccgacgtt tcaaattaaa caaagctggc tttaccatca
    18181 ttccttgcga actgattgac agaaacggcg aacgtctaaa agaaatcatt ttacgctacg
    18241 cagaaaaatg ggatttagaa cctgcttttg ttgagtggtt agaaaaagaa aatgttttct
    18301 gttgtagttt agttgatcgc atcgtcccag gttatcctcg cgaagatgca ggtgttttca
    18361 atcaacggca taactatgaa gatcaattga tggtcaaagc agaaccattc atgctttggg
    18421 tgatcgaagg gcctaaagaa ttggagaatc aactaccatt caaacaggct ggcttgaatg
    18481 tcatcatcac agatgatatg actccttatc gtgaacgtaa ggtgcatttg cttaatggac
    18541 cgcatacagc gatggtccct cttgctttgt tggcggaatt ggaaactgta gaagaagtaa
    18601 tgaaagatcc tttattctcc gcatatgttg atcagttatt caatctagaa ttgattccta
    18661 tgctttcttt acctaaagat gaattagcca tttatgctga ccaaatcaaa gaacgtttct
    18721 tgaatccttt tgcccatcac aaattagaag cgatttcatt aaatagtgtt tcaaagttca
    18781 gcacccgtct gcttccagtg ttcaagaagt atatcgaaga acaaaatcag gtaccaccgt
    18841 taatcactgt ttcactagct gcgctacttc ttatgtatcg aggcgatcaa gtaaagccac
    18901 atgatgatga aaagacgata agcgaattta ctgatgcttg gtctgataat ggaacagcta
    18961 ttcctcgatt attgcaaaac gctgcgcttt ggggagaaga tttgtctcaa ataccgaatg
    19021 tgacagatac agttcaagaa atagctgcaa agatcgatca aaaaggtgtc cggtcagtat
    19081 tacaagaaat gaaaggaacg atggtccatg cttgaaaccc tgcaaccaac aatgaaatta
    19141 aatcgacgag acagtgtggt cgtcgcatta acccctattc caaaacaaac acaactgacg
    19201 attgatcagc aaacgatcac cactcttgaa gatatcccgc aaggtcataa gattgcattg
    19261 ggtgatttga aagctggaga caacgtgatc aaatatggct atccaatcgg tcatgtgacg
    19321 acagacgtaa ctgccggtca atggctgcac actcataatg taaaaaccaa tctttctggt
    19381 gaattggatt atcgatatga aaaagatgtc catccaagtc attacctttt tgagaaccga
    19441 acatttcaag gttacttgcg aaaaaatgga aaagtaggga ttcgtaatga tttgttttta
    19501 gtacctaccg ttgggtgtgt gaatggcatt gctgaactga tcgtcaaaca gttcaaggaa
    19561 aaacatccag atctgggaca gtttgaccat attacgattt taaagcatcc ttatggctgt
    19621 tcgcagcttg gaaaagatca tcaaaatact cgagaaattt tagcggacgc agtaaatcat
    19681 cccaatgccg gaggagtttt agtatttgga ttaggttgtg agaacaatac agttcctgaa
    19741 ttcaaaaaaa ttttaggtga atatgatgaa gaacgagtga aatttctagt ggctcaagat
    19801 gtttacgatg agatcgaaca aggtgtggct ctattagaag agcttttaac agcggcggaa
    19861 aatgaccagc gcatatcagt cccgctctct aagttgaatg tcggtttgaa atgcggcggt
    19921 tctgatggtc tatcgggaat aactgctaat cctttacttg gtgcattttc tgactacttg
    19981 attgctcaag gaggcagcac gatcttaaca gaagtgccag aaatgtttgg cgcagaacaa
    20041 gtattgatgg cacgagcgga aaatgaagaa gtttttgact caatcgttga cttgatcaat
    20101 gacttcaaac agtatttctt gtcttacggg gaaccagttt acgaaaaccc atctcctggt
    20161 aataaagcag gtgggatcac tacattagaa gataagtctt taggctgcac gcaaaaatca
    20221 ggaagctctc ctgtcgttga tgtgctacaa tatggcgaaa aaatcagaaa accagggctt
    20281 agtttattgc aagctcccgg gaatgatctt gtagctgcct ctgctcttgc ttcatccgat
    20341 tgccagttgg tcttgtttac cacaggaaga gggactccgt ttggctcata tgtcccaacg
    20401 gtcaaggtat ctacgaatac aactcttttt gaacgcaaag gtcattggat ggattttaat
    20461 gcaggcgtct tattagaaca accaatggaa gtaatcttag aatcgtttgt tcaaaaaatc
    20521 atcgctgtag ctagtggaga agaaacgaaa aatgaacaaa atgacgttcg agaaattgcc
    20581 atttttaaaa acggagtaac actttgattt tcataaaaca gtataaaaag aaagaaggaa
    20641 gatttatgtt tttatctgat gattttttat taaaagacga ttgggcgaaa aagctttacc
    20701 attcctacgc aaaaaacatg cccattattg attatcactg tcacttagat ccaaaagaaa
    20761 tttacgaaaa caacaatttc agcaatctga cagaagcttg gttaggaggc gatcattaca
    20821 aatggcgctt gatgcgagct tgcggcgtgc ctgaagaaaa aatcacagga aatgcttctg
    20881 attttgaaaa atttttagct tggtgtcaaa ccgtaccaaa aattatcgga aatccgcttt
    20941 acagttggtc acatcttgaa ttgaagcgtt tcttcggtat tgacttgttg ataaatgaag
    21001 aaaatgcaga gaaaatttgg acacaagcaa atgaaatttt ggcagctcct gattttcgcc
    21061 gtcgggaaat cgcaaaaaaa tcgaatgtag aagtgatttg tacaacagat gatcccgcag
    21121 acgacttgca ctatcatcag ttgcttgctc atgaagaacc agatttgaag gttctacctt
    21181 cattccgtcc agataaagca ctcaatattg aaaaagaagg atttaatgca tgggtcacac
    21241 tactggaaga agcagctgac caaacgatca ccacctatca agaactgata gaagtcttag
    21301 ggcagcggat agaatacttc catcaacatg gatgtcgtgt ttccgaccac gctttagaca
    21361 tcttacgcta taaagaagcg gatgaaacag tattagaaga gatttttcaa aaagcacgag
    21421 ctaacaaact attatcttca gatgaaatcg atgcttatcg tacagaaaca ctgatacatt
    21481 tgacccactt ctatcattct cataattgga cgatgcagct gcacatccat gcttatcgta
    21541 actgtaatac acaaatgttg gaaaaattag ggcccgatac cggctatgat ggaatgaatg
    21601 atctttcttt gactattcct ctgcaaaaat tattgaaccg tgcagaagaa acggatcagt
    21661 tgccaaagac gatcctttat tcattgaatc cgaatgacta tcctgcgatt ttagctttga
    21721 tgggctgttt ccaaaaagaa cagaatggaa aactgcagtt aggttctgga tggtggtaca
    21781 acgatactcg agctggtatg cgcgatcaat tgacacaatt tgctgatggt agtgctttgg
    21841 ggaattttgt cggcatgctg acagattcaa gaagtttcct gtcctacact agacatgaat
    21901 atttccgtcg tgttctttgt gaatttttag gcgaaatggt catgcgagga gaagcacctg
    21961 aagatgaaca acttttagga agtctagttc aagcgattag ttatacaaat gctaaaaatt
    22021 actttggctt ttttgcctaa ttgattaata gtaacagtaa cgaatcaaaa acaggatagt
    22081 gaaataacgg aatccgttta tccacgtttt agagaagaag acggaccaaa tttgctccgt
    22141 cttcttctct tgctgttctg cttcattgcc tataaacctt tatgaaagaa gagataacat
    22201 gcctactatc catattgccg gtgattctac tgcggcagaa aaagatgaaa acaagcgtcc
    22261 cgagacaggt tggggagaaa aaatctctgc ttatttttca cccgagatcc agatcaataa
    22321 ccaagcaaag aatggacgca gcaccaaatc atttatccag gaaggaaggt tagcccgtct
    22381 agcttcacag tttcagccga atgatttctt atttgtacaa tttggacata acgaccagaa
    22441 aatccaagaa gaaagaggta cgcttccctt cggtagctat ttaaaaaatc tgcagatatt
    22501 ttttgaatgc gcaaaaaaag cgaacgtcca aatggtctta ctgacatctg tgacgcgacg
    22561 agattactta gaaaacggaa cattgaatcc agatatactt ggagactatc caaaagcgat
    22621 gcgtgcattt gctgagaaac accatattcc tcttttagat gtgttctcaa gatcacaaga
    22681 attgttccag accttctcaa aagaagagac aaaaaaattc tacctgcatc ttatacctga
    22741 tacttgtaaa aattacccag aaggactaaa agacaatacc cactttagtc cagaaggcgc
    22801 tgataaagtt gcccgaataa ttgttgaatt gataaaagaa tcccgtctgc ctcttgctaa
    22861 ttatttacaa aaaggagttt gagatgtttc cattaaattt aggcatccgt gcccacgatt
    22921 tagatgttcg aaatcgtgaa gaaatcgttc aaaaagtatc acagcttcac ttgtcccata
    22981 tccaatttgc accacaaaaa gcatttcctg aacaacttaa attaactgac atgacacttg
    23041 ggagtgctgc ctattttggt gaatacttcc aaaaaaacaa tatcgaactt tccattttag
    23101 gatgctatat caacctatcc agcaaaaatc aagaagttcg tgcacaagca ttggcaactt
    23161 tcaaacagca aattgctctg tgctctgcct atcaagccaa gatgattgct acggaaaccg
    23221 gtagtgtctc tgtcggttac acaaaagaaa attttactga agaagcttat cagatcgcca
    23281 gaaattctat cattgaatta gtcgctcatg cagaaaattt tggcgtaact gttgctgttg
    23341 aagcagggat caatcatcca atctataact atcagttagc taaacgttta atcgatgaag
    23401 tagcttcacc aaatttaaaa atcattttag attgtgcgaa tttgatgcat aaagaaaatc
    23461 atgctgatca ggaaaaagtg atccgaggtg ccttggacaa tttacatggt tatattacag
    23521 cgcttcatct caaagactat atcatggaaa atggaaagat ccgcatggta cctgttggca
    23581 agggctgttt agatttccgc ccggtactgc attatatcaa atatgagcgt cctctaatgt
    23641 acgccactct tgaagctact cctgagattt atgttcctga agcaatagaa cacttgcagt
    23701 ttatttacca agattgttaa ggagaagatt atggaggaac gaatacagaa aagttatcaa
    23761 aaatatcatg caaatgcttt tcagacattg ctgcatcttt atcagggaaa ttattggcgt
    23821 tttgccatgt caggactctt ttttattatc aaacatcttc catcttggtt aatgccgatc
    23881 gctattgcca atgtaatcaa tgcagcttca tcgaaagatt cttctcagtt gcaggccatt
    23941 tatttcaacg cttttttgat ggcttgtctg attggtcaaa atatattgac gaattatttt
    24001 catgtaaaat accatagcac ttcgatccgt caagtagaag ctggactaag agcgaccctt
    24061 atcaagaaaa tccaagaact atctattcct tatcaaaaag aattgcaatc tggacgaatc
    24121 cagtcaaaga tcatccgtga tgtagaagct atccaaacgt tgtcctctca agtatttgtc
    24181 agcggattaa atatcgctgt caatttattc gtagctttag gtatcacgct ttataaaagt
    24241 cctgttgttt tcatgttttt cttattgact gttcctctcg ccgcattcgt tactctcttt
    24301 ttccgtaaaa ggatcagaca gtccaactca gacttcaggc aagaaatgga agagacttct
    24361 gcaaaagtga tcgaagtgat cgaattagca ccgattgctc gagctcatgg gttagaaaaa
    24421 cacgaaacaa ctcgcttaaa taagcatttt actcaaatct atgaaaaagg attccactta
    24481 gatatcctgc aatctgtttt cggatcagtc agctgggcta gttttcagat atttcagctg
    24541 atttgtttga ttttcactgg gatattagct ttacaaggac gcatccaacc cggcgatgtc
    24601 gtgatgtatc agacgtattt cacgacgatc gtcaattccg tttctggaat gatcacgctg
    24661 attcctacga tttcaaaagg catggaatct atcacttcta ttggcgaact gttgattgct
    24721 ggcgatgtag aaacttatca cggtaagaaa aaaatccaac atttggatgg aaattttcgt
    24781 tttgaggatg tttcttttct ttatccggac tcactcaaac cgattatcga tcatttttct
    24841 ctagaggtga aagctggaga aacaattgct tttgttgggc catctggctc tggaaaatct
    24901 acactcctta acttattgat tggatttatc cagccgactt caggaaaaat ttttctagat
    24961 gatcagcctt tggatgaact ggatatgcga agtgtgcgaa attatttagc cgttgttcct
    25021 caaaccactt tgttgttctc cgcttcgatc aaagagaaca tcacttatgg cttgaaaaac
    25081 gtatcaaaag aacgtttaga tgaagtcatc gaagctgcgc aactttcttc actgattgac
    25141 cagcttcctg atggtctgga taccttagta ggcgaacatg gcaacaagct gtctggtggc
    25201 caaaagcaac gtatctccat tgcacgagcg ttgatacgtc agccaaaaat cattttgcta
    25261 gatgaagcaa cctcagcttt agataaccag tcagagaaaa aaatccagca agccctgaat
    25321 tacctgacag aaacacccac tacttttatt gtggctcatc ggctttcaac gatcaaagag
    25381 gcagataaaa tcgttgtgat cgatgaagga aaaatcgttg aaataggtac ttacgaaaaa
    25441 ctgttagctc aaaaaggcta tttctaccgc ttgaaaaatg aatgacaaaa aataggagct
    25501 atgacaagac ttttgtcgta gctcctattt c
//
LOCUS       DBNICI010000022        25517 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.022, whole genome shotgun sequence.
ACCESSION   DBNICI010000022 DBNICI010000000
VERSION     DBNICI010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 25517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.022"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(136..843)
                     /gene="deoD"
                     /locus_tag="AC1ZCC_000743"
     CDS             complement(136..843)
                     /gene="deoD"
                     /locus_tag="AC1ZCC_000743"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIQ1629015.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(860..1678)
                     /locus_tag="AC1ZCC_000744"
     CDS             complement(860..1678)
                     /locus_tag="AC1ZCC_000744"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIQ1629016.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            complement(1702..2868)
                     /gene="deoB"
                     /locus_tag="AC1ZCC_000745"
     CDS             complement(1702..2868)
                     /gene="deoB"
                     /locus_tag="AC1ZCC_000745"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - metabolic compound salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HIQ1629017.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            complement(2967..3923)
                     /locus_tag="AC1ZCC_000746"
     CDS             complement(2967..3923)
                     /locus_tag="AC1ZCC_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1629018.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            complement(3923..5056)
                     /locus_tag="AC1ZCC_000747"
     CDS             complement(3923..5056)
                     /locus_tag="AC1ZCC_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1629019.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            complement(5049..6611)
                     /locus_tag="AC1ZCC_000748"
     CDS             complement(5049..6611)
                     /locus_tag="AC1ZCC_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1629020.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            complement(6823..7905)
                     /locus_tag="AC1ZCC_000749"
     CDS             complement(6823..7905)
                     /locus_tag="AC1ZCC_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HIQ1629021.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            complement(7971..8369)
                     /locus_tag="AC1ZCC_000750"
     CDS             complement(7971..8369)
                     /locus_tag="AC1ZCC_000750"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HIQ1629022.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            complement(8390..9052)
                     /gene="deoC"
                     /locus_tag="AC1ZCC_000751"
     CDS             complement(8390..9052)
                     /gene="deoC"
                     /locus_tag="AC1ZCC_000751"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIQ1629023.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            complement(9098..10399)
                     /locus_tag="AC1ZCC_000752"
     CDS             complement(9098..10399)
                     /locus_tag="AC1ZCC_000752"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HIQ1629024.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            complement(10540..11556)
                     /locus_tag="AC1ZCC_000753"
     CDS             complement(10540..11556)
                     /locus_tag="AC1ZCC_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIQ1629025.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            complement(11864..12469)
                     /locus_tag="AC1ZCC_000754"
     CDS             complement(11864..12469)
                     /locus_tag="AC1ZCC_000754"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIQ1629026.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYATIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12665..13588
                     /gene="coaA"
                     /locus_tag="AC1ZCC_000755"
     CDS             12665..13588
                     /gene="coaA"
                     /locus_tag="AC1ZCC_000755"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HIQ1629027.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            13647..15212
                     /gene="guaA"
                     /locus_tag="AC1ZCC_000756"
     CDS             13647..15212
                     /gene="guaA"
                     /locus_tag="AC1ZCC_000756"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIQ1629028.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15703..17334)
                     /locus_tag="AC1ZCC_000757"
     CDS             complement(15703..17334)
                     /locus_tag="AC1ZCC_000757"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 53 protein"
                     /protein_id="HIQ1629029.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(17348..19021)
                     /locus_tag="AC1ZCC_000758"
     CDS             complement(17348..19021)
                     /locus_tag="AC1ZCC_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HIQ1629030.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(19034..19351)
                     /locus_tag="AC1ZCC_000759"
     CDS             complement(19034..19351)
                     /locus_tag="AC1ZCC_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HIQ1629031.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(19376..20776)
                     /gene="lacG"
                     /locus_tag="AC1ZCC_000760"
     CDS             complement(19376..20776)
                     /gene="lacG"
                     /locus_tag="AC1ZCC_000760"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HIQ1629032.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            21013..21798
                     /locus_tag="AC1ZCC_000761"
     CDS             21013..21798
                     /locus_tag="AC1ZCC_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIQ1629033.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            21801..22493
                     /locus_tag="AC1ZCC_000762"
     CDS             21801..22493
                     /locus_tag="AC1ZCC_000762"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIQ1629034.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(22767..23138)
                     /locus_tag="AC1ZCC_000763"
     CDS             complement(22767..23138)
                     /locus_tag="AC1ZCC_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HIQ1629035.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(23538..23768)
                     /locus_tag="AC1ZCC_000764"
     CDS             complement(23538..23768)
                     /locus_tag="AC1ZCC_000764"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HIQ1629036.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(23862..24200)
                     /locus_tag="AC1ZCC_000765"
     CDS             complement(23862..24200)
                     /locus_tag="AC1ZCC_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629037.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(25128..25244)
                     /locus_tag="AC1ZCC_000766"
     CDS             complement(25128..25244)
                     /locus_tag="AC1ZCC_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HIQ1629038.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT         7436 a         5602 c         4499 g         7980 t
ORIGIN      
        1 ttcacaacct cttggtaaac ggtgttcaaa agcttacatt tgcaataata agtccagcca
       61 agtcaaaaac atgaggagct atttttgctt ggatgttctt attacttaat gcagggcagc
      121 tttttcattt ccttcttatt ctgctatcgc tgtttccaat cctacttcga tcatttcatt
      181 gaacgtagat tgacgttctg cagctgttgt ttcttcaccg gtgatcaaat ggtcgctaac
      241 ggtcatcaaa cttaatgtct gcacatggta tttcgcgcct aagtaataaa gagccgctgc
      301 ttccatttcc actcctaaaa cgccaagttc agctaattgg aacgtctctg tcaaatcatc
      361 tttgtagaaa gaatcttccg aaagaacatt tcctacatga accgtatagc ccttttcttt
      421 agctatctca tatgctttca gtaatagatt gaaatcactg attggaggaa aatgaaaggc
      481 ttggaaattt ttttcgatca tcgaagaact ggtcgctgca ccttgtgcta gtactaaatc
      541 acgaacatgg acgtcttttg acaatgcacc gcatgtacct actcgaatca atttctttac
      601 accatacgac tggatcaatt catgggcata gatggtggct gatggcattc ccattcctgt
      661 tccttgaaca gaaatacgtt ctcctttata tttgccggta aagcctaaca taccgcgtac
      721 ctgattgtaa caaactggat cttccaaaaa agtctccgca atatatttag ctcgcaaagg
      781 atctcccgga agtaaaatct tatcggcgat ctcgccttct ttcgcttcaa tatgcacact
      841 cattttattg ttcctcctat tacaataatg tcaatgtttc tttaaccaat gctttaaaag
      901 attgtttgac tctctgagtt gtttctacaa cttcttcatg gctcagattc gcttgcatac
      961 ctgctgctaa gttggtaata caagagattc ctaacacttt taatccgctg tgagctgcta
     1021 cgatgacttc tggaacagtc gacatcccta ctgcatcggc tcctattgtc cgagacattc
     1081 ggatctccgc aggcgtttca taagttggtc cagaatagcc catatatacg ccttctttaa
     1141 gatccaaacc tagtttttct gctgctgctt ttgctgttgc ttgatagtca ggcgtataag
     1201 cttgactcat atctggaaat ctagggccca ttgcatcttc attcggaccg atcaaaggat
     1261 tttcaccagt aaaattgata tgatcggtaa tcagcatcaa atctcctggt acaaatgatt
     1321 cgttgacacc accacatgca ttagtcacaa tgactgaatg ggcccctagt ccggccatca
     1381 cacgtactgg ataggtcact gtttgcatgg aatgaccttc atagtagtgg aatctccctt
     1441 gcatggctaa cactttcttg cctgccagag acccataaac tagctttcct gcatgcccaa
     1501 ctactgtcga aacaggaaag aacgggatct catcataaga aatcattaca gcatcttcga
     1561 tttcatctgc taattcacct aatcctgaac ctagaatcaa accaaaatca atttctttta
     1621 ctcctttatc aaggataaac tgggtcgttt cttgtaacat ctcgcttaac ttggtcattt
     1681 gctttctcct atttaaaaag attattttaa taatttcaag aagctttcgc cattttcagt
     1741 cgaagaaaca ctaaagttct cagcaatcgt tgctgaaata tctgaataat atccttgagg
     1801 caatgcccct tgttctttca tttttttact gtaaaccaat aacggtacat attctcgcgt
     1861 atgatcggta ccagtaaacg tcggatcgtt tccgtgatcg gctgtaatca ttagcaagtc
     1921 gtcttcttcc atcgcttcaa gcaattctgg aattcgtaag tcaaattctt caatcgcatg
     1981 cgcatagccg actacatccc gacgatgacc gtataatgca tcaaaatcta ctaaattggt
     2041 aaagctgatt cctgaaaaat cgtttttcat aactttcagc agctggtcaa cgccatccat
     2101 attgcttttc gtgcggacag attcagtgat tccttgtccg ttgaaaatat cgttgatctt
     2161 tccaacagca atcacgtcct ttcctgcttc tttcaaagaa tcaaggactg tgtgtccgaa
     2221 tggattcaac gcataatcat ggcgattgct tgttcgtgtg aagtctcctg gttcacccac
     2281 gtacggacgt gcaatgattc ggccaatcat atacggatca tcttttgtta tatcacgaac
     2341 gaattggcaa atccgatata attcttctaa aggaatgatt tcttcatgtg ccgcgatctg
     2401 aaggacagga tcggcagaag tatatacaat taaatcgcct gttttcattt ggtgttcacc
     2461 gtaatcgtca ataacagctg ttccgctata aggtttgtta catacgactt tacggccaga
     2521 aaattcttcg atttgtttga tcagatcatc agggaatcca ttaggaaaca cacgaaatgg
     2581 agttttgata ttcaatccca tgatttccca gtggcctgtc atcgtatctt tccctacaga
     2641 aacttcttct aatttcgtcg catagccttg atgatctttc actgattcca caccttcaag
     2701 aggacggatc gttcctaaac caagctgctc taaatgtgga acgttcaatc ctgcttcttt
     2761 cgcaatgtgt cctaatgtat cagaaccaac atcaccgaac ttatcggcat ctggtgcttc
     2821 tccaattcct actgaatcca tgacgatcag atgtacgcgt ttaaacatga ttattccttc
     2881 tttcttttta tttttattgc ctttaaaaca aaaagggcga gacgaaaaac acttcccgta
     2941 acccaccctt tttattcgct aaaagattat tttgatttga tatagttctt cccgtttgct
     3001 tttggaccag aagctttccc taagaaaaca accagcacaa tgattgtcag caaatatgga
     3061 gcaatttgca gcaatactgg cggaatcgaa gaaataactg gtaattgtgc gccaatgata
     3121 ctcaagcttt gagcaaaacc aaagaacaat gaggcaccca ttgcccctaa aggattccac
     3181 ttaccaaaga tcatagcagc catggagatg aacccttgtc caacgatcgt accagctgaa
     3241 aagttaccgg aaatcgactg agcaaaaaca gcaccgccca taccgcctaa caagccagaa
     3301 atcagtacgc ctgaataacg catagcatat acattgatac ctaatgtgtc ggcagcttga
     3361 ggattttcac caactgaacg gaggcgcaag ccgaatttcg tcttgaagat gatcacccaa
     3421 gctaggacag caatcaaaat agcacaataa gcaggtaaga atgtattttt aaagaaaatt
     3481 ggtccaatga ccggaatatc tgctaatcct gggaaagaaa aataccccag attctgcgtg
     3541 atgttatcag tttgaccttt accataccat gctttgatca agaaaacacc taaagcaggt
     3601 gccatcagat taattactgt cccgctgata atatggtcag cacgcaaact gactgttgct
     3661 actgcgtgaa tcagagaaaa taggatacct acgacgccac ccactaaaat agcgatccat
     3721 ggtgtcatag aaccaagtga tggactcatc gtcaagttga aaacgatgga actaaaagca
     3781 cccatgacca tgattccctc tagccctacg tttacgatcc cgcttcgttc agaaaaagtt
     3841 ccgcctagtg acgttagaat tagcggtgtt gaataaacca gtgtagaagt aaggatcaag
     3901 gcaattaatt cgatagtcga cattatagtt cgcccccttc atggttttga ttcgttggtt
     3961 tcgattctat tgtcgcaacg atctcttctt gttttttccc ttggaagact ttagccatga
     4021 cgaaacgaat caggaagttg atcccgacaa agaagataat caaagcaatc gatacattga
     4081 cgatctcaaa cggaacacct gctcctgtct gcatgccaag tccaccgatc ttcaaaatac
     4141 tgaacaagat cgctgataaa agaataccga ttgctgatcc agaaccaagc aaggaaacag
     4201 ccattccgtc aaatccaata gatagcgaag tcgtttgaac aaagaagttt tgatacgttc
     4261 ctaatccttc aaccacaccg ccaagccctg ccaaagcgcc agaaatgacc atcgacaaaa
     4321 cgatcgtacg tttactactc attcctgcat actcagaagc aaaaggattc aatccaacag
     4381 aacggatttc aaatcctaaa gtcgttttct tcatcagcag ccaaacgaaa ataagcgcga
     4441 tgattgctaa aaataatcct gagtttacgc gagagccgcc aaaagcttgt gttaaccatg
     4501 gtaaccgcag tgaggcattt tgactgatca tattggaaga gtccaaactt gagcgatagc
     4561 tttcaggcat cacttcatgt aaagcaaatg tgcttacgta taataagata tagttcatca
     4621 tgattgttac gatcacttca cttgttccaa aaaatgctcg aagtaaacct ggaacagccg
     4681 ctgccatcgc accagctaag gcacctaaga tcacgcataa cggaagcaaa acaacttttg
     4741 gcagatctgg aaacgcaagt gccacccaga cactgacgat ccacccgcaa agtgcttgtc
     4801 cagaaagccc aatattgaaa aacccagctg agttagctac agaaaaacct aatgctgtaa
     4861 aaatcaacgg cgttgcttgc cttaacgttt cacctatccc tctttgcgaa ccaagggctg
     4921 catctagcat agaagaataa ccttggatgg gctgaaaacc aaaagcagcc atcaatatcg
     4981 cgcctaagat caatcctaaa acaactgaaa gtacaggcac taataaatta cggaattttt
     5041 cgctgcgatc attcatgtgc gccagccccc ttttctaact cttttcgtgc ttcttctaag
     5101 gtatagcccg ccatcaatag acctaattca ttttcagatg tttctttcgg atcaacgata
     5161 ccgacaattt ctccttcatg gataacagca atacgatccg aaacatttaa aatttcttct
     5221 aattcgaaac tgatcagtaa cactgctttg tatttgtccc tttgttcgat cagacgttta
     5281 tgaataaatt cgatcgcacc tacatccagt ccacgagttg gatttgcaac aatcaacaga
     5341 tcaggatctc ggtcgacttc acgagcgata attgcttttt gctgatttcc gcctgataaa
     5401 gcttttgcgg gaacaagttc attcgttgtt cgaacatcat attcttcgat caattctctg
     5461 gcatgttcat tcattactga ataattcagc atgccgtttt tactgtaagg tttttgatga
     5521 taggtttgta gtgcgatatt ttcagataat gtcatatcta ggactaaccc gtatttatga
     5581 cggtcttctg gcacgtgtcc tacaccatgt tcagtgattt ttcggggttt tttattagtg
     5641 atgtcttccc cttttagctt gatatgtccg ctttctgcct ttcgcaaacc agtcaaagct
     5701 tggatcaact ccgactgccc gtttccatcg attccagcga taccaagtac ttcgccagca
     5761 cgaacctcta agttcaagtt tttcacggct tctaatccac gattttcttt caccactaga
     5821 ttttcaatag acaaaacgac ttcttgaggc ttcgcttctt ttttcatcgt tttgaatgaa
     5881 accgttcttc caaccatcat atcggctaat tgttgtgagg ttacgtcttt aacgttgact
     5941 gtaccgattc cttttccacg gcggataact gtacagcggt cagctactgc tttgatttca
     6001 tctaacttat gcgtaatcaa aatgatcgac ttgccttctt tgactaattc cttcatgatc
     6061 acgattaatt catcaatttc ttgaggggtc aatacagctg tcggctcatc aaaaatcagt
     6121 acatctgctc ctcggtaaag tgtttttaaa atttctaccc gttgttccat tccaactgaa
     6181 atatcacgaa cgtaagcatc tgggttgact gacaatccat actgttcaga aactttttgg
     6241 atctcttttc gtgctttttt atggtcaagc atccctgcgc gactaggctc gcttcccaac
     6301 acgatgtttt ctgttacagt aaaggcatca acaagcataa agtgctgatg gaccatccca
     6361 atccctaatt ggttggcttt ggtcggactt gtgatcttta cttctttccc acgcatcaaa
     6421 attttccctg atgtcggctc taataatccg gaaagcacgt tcatcaatgt agatttgcca
     6481 gcaccgtttt ctccaagcaa cgcatgaatt tctcctgcct ttacttgtaa attgatgttg
     6541 tcatttgcct tgaatgttcc aaattctttg gtaatattct gcatttctat gacaatattt
     6601 tcttgaacca cctttttttc acatcctaac gatttctatc tagggcaacg attgactaag
     6661 attcacgctt agtgagcgtt cttcttttga aacaaacatt acttcaggga gttgttccct
     6721 tatccaaact gtcacaaggc agcttggata aatgaacaat tcttgttcta atcaaaccat
     6781 agccttgtca aacatgtgtc taacaaggct atagaaatgg ttttagtttt cttcaggagt
     6841 ttctggaact tttacgtcac ctgaaatgat tttttctttt gcttcatcaa ctgctttttt
     6901 cgcttcgtca gaaagttgtc cttctgttag accaacaccg tcttctttca atccataaac
     6961 agtgtgttcg ccgccaggga atttaccgtc cgctgatttt tgtgccaaat cttctactac
     7021 tgttccaact gctttcagtg tagatgtcaa tgtgaagttt ttcttttcgc cgtttaatgt
     7081 gtattcgcct tcgtctgatt ggtcgcggtc aacaccgata acccaaactt ttttgtcgcc
     7141 tgattcgttc aatgattttg cttcttggaa tacaccgtta cctgtaccgc ctgaagcatg
     7201 gaagatgata tctgcgtttt gtgcatacat accttgagca attgaacgtc ctttatctgg
     7261 agcagagaag tcaccagcat attgattcaa tactttgatt tcttttccta attctttcgc
     7321 acctgcatca acaccggctt tgaatccagc atcaaaacga tcgattactt caccttttac
     7381 accgccgata aagccgacta cgtttgtttc agtcgtataa gctgctgcaa cacctgcaag
     7441 ataagaagct tcgttgtctt tgaaagttgc agatacaacg ttatctaatc catcgatgac
     7501 atcatcgata ataacaaagt tcgtacctgt attgtttgtt gcttgttctt ggatcgctgg
     7561 ttttagttta taaccaatac caaaaatcgt tttgaatcca gcattcaatg cttggtcaat
     7621 atttgggatg taatctgatt catttgaaga ttggaaatat tggaagccat catttcctcg
     7681 tgaaagtcct tgatctttcc cccatttttc tagtccttcc caagctgact ggttgaatga
     7741 acggtcgtct acaccacctg tgtctgtgat caatgctgca gttgttgtgt ctgatgagct
     7801 tttgccaccg gatgaatcag tagagccgtt gttgccgcat gcacctaaaa ataaccctgc
     7861 tgctagtgcg acagcaccta aaccaaataa ttttgctttt ttcatttctg taaagcctcc
     7921 taaaataatt gttttatttt tcaacagtca gctgactgaa tgaacatgag ttatagatct
     7981 ttttcagtaa atgcataagg cagcagtgca cgaacggtcg ttgccttcgt cacaccattg
     8041 tctcctacta gcgtaaccgg catatcaggg gaacagaatt ctgccattac ttgtcgacat
     8101 gcaccacaag gagagatagg atcgggggtg tgccccgcta cgactagatg cgtaaatgtt
     8161 ttttctcctt cagatactgc tttgaaaaaa gctgtgcgct cggcacaatt cgttaatcca
     8221 aatgatgcat tctcaatgtt gatgccttga taaatcttgc ctgattccgt tacgagacaa
     8281 gcgcctaccg gaaaatggga ataaggaaca taggctttat ctaacgcttc aatagctgtg
     8341 ttgatccatt cttgtttgag ttctgccatg agcttctcct ttgaaaaaat taatagcctt
     8401 caccagttga accagatacg attgctactc ctgcgcttgt tccgattctt gtagcaccag
     8461 cttcgatcat tgccattgct tcttcttcgt tatggatacc gccggaagct ttgacaccca
     8521 tttctggtcc tacagtttca cgcatcaaac ggacatcctc tacctttgct ccgcctgttg
     8581 agaaaccagt tgatgttttg acaaaatcag cgcctgcagc ttttgctatt tcgcaagctc
     8641 ggatgatttc ttctctatct aataacgatg tttcaataat aactttgaca agcgcacgat
     8701 cttttgctgc ttcaacaact gcttcgatat ctttttggac ttgttgatca tcctttgatt
     8761 tcaaagcacc gatattgatg accatatcga cttcatctgc accgttattg atggcatctg
     8821 ttgtttcaaa tgcttttgtc tcgggtgtgt ttgctcctag aggaaacccg attactgtac
     8881 atacggctac tggctcacct tgtaattgtt ctttcgctag cgcaacccat gctggattga
     8941 tgcagacaga ataaaaatgg tatttttttg cttcttcgat aatttgcatc acagcttctt
     9001 tagatgcgtc tgctttcaaa atagtatggt cgatcattcg gtttagttcc atttcttttc
     9061 cccttttctt taaaacggta aagtccgctt ttttccttta ttctgtgatg atgtcatgaa
     9121 tcaacgttgg cttcattgcc gattcaccaa tttcaatatt tttatataat aattcaatga
     9181 cagaagtgat ttcttcgtcg ttactataga tcgtcaatag tgactcacct tttgtcacag
     9241 tgtcaccaat ttttttatgc agtttcaatc caacagcgtg atcaatatct tcttctttgg
     9301 ttttacgccc agcacctagc atcattgcag caattcctaa ttcatttgcc acgatttttg
     9361 acacaactcc gctttgttta gcaggaagct ccattgtgta gcgtgcagtc aaaattcgtt
     9421 ctggatgttc cacgatcgct gggtctcccc cttgattttg gatcatttct ttgaattttg
     9481 ctaacgcttt acctgattcc agagcttctt gaagcaacgt tcttgcttca tcggttgtct
     9541 tagctttgcc agcaagaaca accatttggc ttcctaaggc gtagcacatt tctactagat
     9601 cttctggacc atttccttgt aatgtttcga ttgcctcgac aacttctaga ctatttccga
     9661 tagcttcgcc aagaggttct gacatatctg agataaccgc cactgtttct cggttagcca
     9721 gtttcccaat gctcgtcatg gtctttgcca gacgtcttgc atcttcgatg ttcttcatga
     9781 aagcaccgtc tccagtggtc acatccaaaa cgattgcatc tgctcctgcc gcgatttttt
     9841 tactcataat cgaactggca atcagcggaa gagaatcaac ggtcgctgtg acgtcacgca
     9901 aggcatacaa ttttttgtcc gccggtgcca agtttcctga ttgaccaatt accgctactt
     9961 tactttcatt aacaatgcgt acaaattctt catccgaaag ctcgatctga aatccgggaa
    10021 ttgcttctag cttgtctagt gtaccacctg tgtaacctag accgcgtcca gacattttag
    10081 ccactgtcac tccgacacta gctactaggg gggctaaaat caatgtggtc gtgtcaccaa
    10141 cgccacctgt cgaatgctta tctaccttta ttccttcaag agaactcaaa tcgatgacat
    10201 caccagaatt agcaatcgcc aatgttaagt tcgtgatttc ttcatttgtc atgtcttgat
    10261 aaaaaattgc catcaataat gcactgactt gataatctgg aatctttcca ttcgtatagt
    10321 tcgtgacgat gtactcgatt tcctctttcg acagctcgtt tccatccctc tttttttcaa
    10381 tcagatccac cattcgcatt gcctgatcct ccttgtcttc attcggtatt atacattatt
    10441 tttaggaaag gtaaaccact ttagtaaact ggtttactaa aaataaaaaa agcactaact
    10501 agcctttgta aatgcttaca taagcaatat accgccgagt tagccctttg tcaatacttt
    10561 tacgctctca cgaataatta attttgtttc gaacactttg ttcggaactt ttctttgagg
    10621 aaattcgatc gtatcgatca ggaaacgtgc agcataaaat ccaatatcaa aacttggctg
    10681 ctgcactgtc gtcaattgcg gaatcatgta ttctgacagc tttaatccat cgaatcctat
    10741 gactgagata tcttctggga tttttttgcc caattcatcg atcgcctgat agcatccgat
    10801 cgccatggca tcattcccgc aaaaaatagc cgtgatatct ttatgtttga gtaatttttt
    10861 tgatgcagta tatccacctt ggacagtcaa ctctccatgt tcaacaaatt tcccattaaa
    10921 aggcaggcca tgatctttca gcgcatgacg atatccgttg aaacgttctt ctaattgata
    10981 gtatccagtg gtttccttca gcatgccgat cttcgtatgg ccattttcaa taaacagcga
    11041 aacagcttga taggcgccct catattcctt tacgataagc cgtccgttat cccgacgatt
    11101 caatccgcga tcgattaaaa tgattggcat tttttgataa aaaccgctgt ctaatgcata
    11161 tttctcaggt aaaatatttg gtgtggccag caagattccg tccactgagc ggtgggaaag
    11221 ctcttgtaaa tacttgatct cattttcttg gctatgacga gaattgcata acatgatcac
    11281 atagcccaaa ggattcaaat agctctctac accttcgatc agtttggaga agaagaaatc
    11341 agtgacatca gggacgatca ttccgatggt ctttgaatgt cgattgatca catttgaagc
    11401 gaaataatcg ggcttataat gatgttcatt cacgatcgac aacacttttt ttcttgtctt
    11461 atcactaaat ctgcttcctt tgttattcaa aatctgtgag acagttgtta ctgatacgcc
    11521 tgccatttct gcgatatctc tgatggtcag cttcatccta ttggctccct tctatctaaa
    11581 ttccctatca gtgtaccaaa acccccaaga aaagaataga gaaaaaagga aagtatgttt
    11641 tttgataaaa acctccttta tctaccgact tggcagcctt ctatacaagt tctgtatctt
    11701 atttatttta tcgtaaaaaa caacgactct gttcctacta tttctttttg aagaaaaaat
    11761 agttctttgt ttttaaaaac ttctcaacaa aaaaagaact gatacgtacc cgtacatgca
    11821 cgcccagttc cactctttcg tcgctccact atttttcagc tagttattct ttcacacttt
    11881 tgatgatata atatccttta tcttttgtga cgatttctgc attaccaaat gtttcttcca
    11941 ttttcttttg cgcactcggc gctccttgtt tcttctggat cacaattgtc agtgtaccgc
    12001 ccttcttcaa ccgaggatat gcgcctgtca aaatttcatg tacaactttt tttccagcac
    12061 ggatcggcgg attgcttacg atagttgcat aggtttcttc atgaactgct tcatagatat
    12121 tcgattgatg gatatcaaca tgttcgattt ggttacgctt tgcgtttcct tgcgctagac
    12181 ttaccgctcg ttcattaatg tcgaccattt caacaaaccg ctgtgtcgca aatgccaaag
    12241 ctaacccaat cggtccatac ccgcatccca catctaagat ctttccttca tcaggtaact
    12301 cttcccaatc gaaagcatca atcaggacac gtgaaccata atccactgtt tcacgtgaaa
    12361 atacaccaga atctgttaca aattgaaagg ttctgccttt taactcaaaa gcccattgtt
    12421 caaaatcgtg cgccgtatct gggcgttcag aatagtaatg atttgtcatg tcttccatcc
    12481 ttctcatttc tttcatgaca acagtctatc cttttatgta tccgtgcgca agtcaagaat
    12541 aagactccgt ttctttctat tataaagatt atacaaaaag atcggcaatc tctcttaagt
    12601 ggtggggttt gcgtttattt tgtgttaaaa tagttccatt gatgggagaa cgcaggaggc
    12661 taatatgaat gatccaatga actactaccg tgtggcaaga gaagaatggc gagaatttta
    12721 tcgtaatggg caagcacctt tgacacaaga tgaactagat aatatcaaaa gtttgaatga
    12781 ccgcatttcc atgcaggatg ttcgagatat ctatgtacct cttgctcatc tgatttatct
    12841 ttatatgaaa gaatttgaat ctttgttctt gagtaaaggt ttatttcttc ataaatacgt
    12901 agaagtaccg ccttttatta tcgggatcgc cggaagtgta gccgttggaa aaagtacaac
    12961 agccagactg ctgcagacga ttttgtctcg taccttcaaa cgaagaaacg ttcagctgat
    13021 cacgactgac ggatttcttt atccaaatcg tatattaaaa gagcgtggaa tcatggaccg
    13081 taaaggattt cctgaaagtt atgatatgga gatgctgatc gatttcttga accgtgtaaa
    13141 atctggtcag gaagagataa aagtacctgt ttactcacat gatgtgtatg atatcattcc
    13201 agacaagtac gaattgatcc agcagcctga tatcttgatc gttgagggga tcaatacgct
    13261 tcaattacct gcgaatcagc aaatctacgt cagcgacttt tttgattttt ctgtctatgt
    13321 cgatgccgat cccgtgctga tcgaaaaatg gtatttggat cgttttggtg cgttactgga
    13381 tacggctttc caaaatccag agaattatta ttatcgtttt gctatcggag atcgtgaaga
    13441 agcctttaaa atggcaaaag atgtatggaa aaatgtgaat ttgaaaaatt tacaggaata
    13501 tatcttgcct acacgaggaa gagccgatat aattctccat aaaactgaaa atcatatcat
    13561 cgattcgatt ttcatgcgaa aatattagca ttttaaaata gtttgactat tttgagataa
    13621 aatttaaagg gataggggta ataaatttga caaacgttgc cgatatgacg aacgttgaaa
    13681 agattatcgt attagactac ggtagtcagt ataaccaatt gatcacaaga cgtattcgtg
    13741 atcttggagt cttttccgaa ttattgagcc accgcattac tgcagaagaa gtaaaagcga
    13801 tgaatccaaa gggaatcatt ctttctggcg gaccaaacag tgtatatgat gagaatgcat
    13861 ttgggatcga tcctgagatc ttcaatttag gtattcctgt attagggatt tgctatggta
    13921 tgcaattgat cacgtacaaa cttggcggtt ccgtagagcc tgctaaaaac cgcgaatatg
    13981 ggaaagcctt gttagaagta acaagcgatc aagctgtctt gtttaaagaa acaccaaaac
    14041 aacaaacagt atggatgagc catggtgact tggtaactaa agttcctgaa ggctttgaaa
    14101 cagtcggaac aagcaaagat tgtccgattg cttctatcca agacacaaca cgtaattttt
    14161 atggtatcca attccatgca gaagtacgtc attccgaata cggaatcgaa ttgctgcgcc
    14221 actttgcctt tgatgtttgt cactgtgaag gtaactggac aatggataac tttatcgata
    14281 tgcaagtaga aaaaatccgt gaacaagttg gtgacaaaaa agtcttgctt ggtctttcag
    14341 gtggcgttga ttctagtgtc gttggggttc ttttacaaaa agcgatcggt gaccaattga
    14401 catctatctt tgttgaccac ggtctgctac gcaaaggcga agcggaacaa gtaatggaaa
    14461 gtctaggcgg aaaattcggc ttgaacatca tcaaagtcaa tgcaagagat cgcttcttag
    14521 acaagttgaa aggtgtatct gacccagaac aaaaacgtaa aatcattggg aatgaattcg
    14581 tatatgtttt tgatgacgaa gcaacaaaac ttgctggtga agaaggcgtc tctttcctag
    14641 cacaaggaac gttgtatact gacgttatcg aatctggtac agaaacagca cagacgatca
    14701 aatctcacca taatgtaggt ggactgccag aagatatgca atttgaattg atcgaaccat
    14761 taaacacatt attcaaagac gaagtccgtg aattagggac tcagttaggc atgcctgatt
    14821 caatcgtttg gcgtcagcca ttccctggtc ctggtcttgg tattcgtgtt cttggcgaaa
    14881 tcactgaaga caagctagaa atcgtacgcg aatctgatgc tatcttgcgt gaagaaatcg
    14941 cagctgctgg acttgaccgt gatatctggc aatatttcac tgttcttcca gggatccgct
    15001 ctgtcggtgt tatgggtgac ggacgtactt acgactacac agtcggtatc cgtgctgtaa
    15061 cttcaatcga cggaatgacc gctgactttg cccgtatccc atgggaagtc ttgcaaaaaa
    15121 tctctgtacg tatcgtaaac gaagtagccc acgtcaaccg tatcgtgtat gacatcactt
    15181 ctaaaccacc agcaacagtg gagtgggaat aaagagccaa cataatatgg agggggaatc
    15241 cctataaatc aacattcttc aatagctaaa atgcttgatt ttcattaaaa ataagatggt
    15301 tccctaccaa aagagactat aatataagtt aatttgtttc attcttatca taaaaagaag
    15361 ttctcgaaaa acgaactgtt ttccgagaac ttttttctat ccatctaagt gctcaaatca
    15421 tattcacgac ggaattctaa ggttaccctt tcttaaacaa actttatgtt atctgcacaa
    15481 ctttttgcaa ttttttcatt gcttcttacg aaatcgttga actctcttta gcataataca
    15541 aagtgtagat gaagtctggt gctttactcg ttttggtaaa aatgactgga aatcgtttct
    15601 ggttattttt ggcaaaggcg aggtgcaggt tcgattcctg ccttctacgc taacgatgtg
    15661 gctgaaggga acctccgatc ttgaatcgaa acgatccgca gtctattgtt tcaccaatga
    15721 aacaaaatca acgttcgtcc aagcatttcc tgaagccttg ctataaaccc caatttcaca
    15781 aacacctgtt gtaattggaa tgtctttgat tgtaattttt gtccattcac ttgttttaac
    15841 aaccgcagta tttagttccg ctccaccata atttttgata tataactgat tagtatcaaa
    15901 ctctccccca gacattacat aggcactcaa tgtgtaagtt cctttgctta tgccactaac
    15961 attttgataa gctgatgcct cgtatgcttg atcctgccaa aaagttagtt tgtagtcccc
    16021 aaaaaaagtg cccgcttccg ttttgatcgt acccttagcg gttccttgtt gaaaccaggt
    16081 ttgccaattg gaaggactgg tcgttggtcc atcgctttcg aaacttgcat ttttaactag
    16141 atttatttgc gcccctgctt gtttaaacgc acgcaagctt ggcaaggcgt ttccagcaaa
    16201 atcaaacatc gcttggttgt cccattcgtt tcccatagtg gattgaacac tgagataatt
    16261 catacctgct tgtgttgccc aactcacatt tccgttgtac caaagaggtt cccaatagaa
    16321 gaagcccgcc ccgcgattat tcggtactgc ctgaatccga tcggcaagat ccagcaaata
    16381 ctctgcttgt cctgtcggtg ttggttcata tccccccgca gctgcttcgg cttcaccaaa
    16441 agcatttggc atagtatcgg cattcgctgt tgtatgccca taagctgttt caactacgat
    16501 cacttcttta ccataccgtt ggctgatcgc attcatattg gtttgaagtt cattcattga
    16561 accgtgccaa tacggataat acgacactcc aattaaatca aatgacacac ctcgactcgt
    16621 gatttcatca aaccaccaag taaatgctcc tgtatcgcca ccatcagcta agtggagcat
    16681 tactggagtt gttttaggtt gagtgtctcg aacagcttgg ataccagact tcaaaaaagc
    16741 agccaatcga tcaaattctc ccccgccttc tccccaagac ttgccataag gccagagcat
    16801 tccgctattc agttcattgc cgatctgaac catatcagga taaacgtcca gctgcttcat
    16861 ttccgacaga acgttctttg tatagtcgta caatgccgta ttcatttctt caaaggtcag
    16921 accttgccat gctttaggaa gattctgttt cccaggatca acccaaaaat cactgtagtg
    16981 aaaatccagc agcaccttaa gtcccagatt ttttgctcgt tttgcaaggt caatcgtcgt
    17041 ttccagatca tttgtccctg ctccataact ttctccatta ctattataag gatcctgcca
    17101 caaacgcagt cgaacatagt tgacaccatt attttttaaa atctctaagg catcttttga
    17161 aatatcgtct tcataatatt ttgcaccaga tttttccata tctgacagga tcgaaatatc
    17221 tgctccgcga ataaattcgc tggcagatgc ttcaacagtt tttgaaacag cccccaaagc
    17281 ccctaacaca gctgcaaata aaacagcttt acgcaaccaa attcccattt tcattatggc
    17341 tcctccttta ttgaaattgg ttcagaataa actgaacagc tttttcagga tctctcgtta
    17401 atccgatata ttctgctcct tttgtcgcaa ccagcttgat tcctaagcga tcggtgtctt
    17461 ctttgatatc atcatagtaa gaattgactt gcggtgctaa gaccaccata tcaaaatcct
    17521 gcagcatttc gtaatgagaa ccataagcac ccgccattgc actgatcggt gctttcgatt
    17581 cagatgcacc tttttttaaa gcattagcta acatcgcact tgtccctgcg ccagcacaaa
    17641 gtaccaacac attgatttct ttatttgcat cgctgataac ttgactcgct gccacaggta
    17701 cttcctcacg tacatcggca gaagcacctt cctcggttgc gatcacctct gctttttgtg
    17761 cttctttctc tactaagaca gcatcgtatg ctttgcaaaa tggtagatag atcaggaagt
    17821 caacgacgat tagaaccgct gctagaacaa aagctaagat acccatgccc gttcctagaa
    17881 ccaaaccgat cggtgctgga gttgcccaag gcaaaacgta cataaagctg ttcatgccca
    17941 atacatcgac aaaaaacttg aacaggcaaa cgtttacaac aggtgttaac aaaaatggaa
    18001 taaaaaaata agggttcaaa atcatgggtg ccgcaaatag caatggttcg ttgacagcga
    18061 aacttactgg aataaaggaa gctttcccaa ctgccttcaa ctgcttagac ttagcaaaga
    18121 gtaggaagat aaaaggaaca accaatgttg ccccagttcc tcccatagtt cctacaaaat
    18181 tccctaaacc caccgtcagt actttagaag cttgttcacc ggaattgaac aattgcagat
    18241 tggtttccac attggcatag ataattgccg caattgccgg ctccacgatc gacggtccat
    18301 gaacaccgac aaaccaaaac agcgccatcg ctccccagat aatagcgatt cctaagtacc
    18361 cgtcagctgc tgtaaatagc ggctgcagca ccgtaatgat tgcttctgca aatgagattt
    18421 ggaagatttg tcgaatgcct aaatcaatta aaacggccgc aaatactgaa aaagagaaag
    18481 gaaaaatatc tcggaaggtt tgcgaaatcg ttccaggaac ttctttaggc attttaatgg
    18541 taatatcttt cagcacacaa aacttatata aattaactgt taaaaatgca gcgacaaagg
    18601 aagccagtag tccctttgtc cccatatatt ccgttgaaaa tccaccttca atcggagaga
    18661 cgcttaacaa caggaaactt accacagagg ccagcatcac agaggtggtg ttgattactt
    18721 tgtttttcgg taagcgacga ttcatcgaat cagccaagca gcgagcagtg gttgctgaaa
    18781 cgagtaaccc tacaacaccc atcgaataat tatagacttt ccatagccat gtggagacag
    18841 attcaggcca ttggtaacca aaaatctcag gaaccgcagc aatcaaaatg aaaatgcttg
    18901 aaaataaaat agctggcatc gctgttaaaa atccgtcacg aacggctcct aaataactat
    18961 tacgtgagat tttttcaaaa aatggtttcc ctttttcgat ttgtttaatg attccatcca
    19021 tagcgttctt ctcctatcct tgattctgtc tgtacagtac aataaagtct tgaatcagat
    19081 ctctcagcaa taaagtggtc attaaatgat cctgcccatg gataaaaatg aatcccatct
    19141 ccatctcttt cccagatgct tcttccgcta aaatttttgt ttgcgaatta tgtgcaaggg
    19201 ttaaattttc gtcggcttct ttcaacgccg aatcaacatg ttcaaagttt ccttgtctta
    19261 cttctttcaa taatttcaat aatgtactgc gagcgtcgcc tgaatatgca acgatctcaa
    19321 aaccaagcat ttgtaattcc tctttattca tcttttcttc ctccattatc acttattaaa
    19381 taatcgtttt ggtttcactg accaacttgt accagtaagc tgattctttt gggtaacgct
    19441 tctgggtttc aaaatcaaca taaaacaagc catacctttt gttataccca tttgtccatg
    19501 agaaaagatc cataagcgac cacaaaaaat accccttcac atttaccccg gctgttatcg
    19561 ccttgcttaa ggattccaaa tagactctca aatagtcgat tctcggctga tccataataa
    19621 tgccatcttc aaactgatct ttatacccca tcccgttctc agtgatatag attttgttgt
    19681 aatgtgggta atcgctttta attcgcagca acaagtcata tagaccttct ggataaatca
    19741 gccagtccca atcagttcga ggaattcctt ctttataaat acgttctccg attcccttca
    19801 ctttataaac agaggtccct ttttcacccg taccgttatg atggattgca ttttctccat
    19861 cgtaagcttt gacaaaatgg cattggtagt gattgatccc taagtagtca ttacgcgtgg
    19921 atgctttttt caattcaacg aaatcttctt caggaaaatg atagctggcc tgatttgctt
    19981 cacatatctc atcaagagca gtcaaggttt cagttgaata atagcctaaa taggtagcat
    20041 ctaataagaa acggatcgac aatgcatcgt ctaaaaaggc agcatgctta tcttccggcg
    20101 cgtctgttgc cgcatatttc gtctctaatg agtgaactac accaatttca cccggaagtt
    20161 cgttttcttt gaaatagttc actacacggg catgagcaac catcatatta tgaagacagg
    20221 cgacgatctt tgtaaaatca tatttgattc ctggtgggaa aacacctaac aaatattgat
    20281 tggttgcaac tggatagatt tcattaaagg tactccacac cttgacttct ttgaattcat
    20341 gaaaacaaaa aatggcatag gaaacaaatg cttctattgt ttctcgattc aaaaaatctc
    20401 catggtcaaa caaacgttta ggggtatcaa aatgatgcaa cgtcacaaac ggcgttacgt
    20461 ttcttttctt acattcttcg aacacacgat gataaaaagc gattccttct ggatttggtt
    20521 tcccagctcc atcaggaaaa atgcggctcc aagcaatgga taatcttaga ccatttacac
    20581 caaaacgttc acacaattca atgtcctttg catattgatg ataaaaatca cttgccggat
    20641 cgggactgaa tcgcccttgt tcctctaaaa agtcatccca agcaacagcg cctttgccac
    20701 cttccttagt ggctccttca acctgatagg ctgctgtggc tccgccaaaa ataaaatctt
    20761 ctggtaattt aagcatgtac atctctcctc aaatctataa tcagttttcg ctctttctaa
    20821 ctgattgcat tgtaaacgca tccaaaacat ttgtcaacaa aaacaaacac aaataatcat
    20881 aaaaaccaga aaacagcaat aaaatctatt gtaacaaaca taaaaaccac cctaaatgtt
    20941 tacttttgtt cgaattagat tgactgttgt ccgtttatat gatagattat ccttaaatta
    21001 gaggtgaaga caatgttgaa aaaagaaaga ttgcttacta tcgtagaaat ggtcaataaa
    21061 aaaggaatcc ttaccgttaa tgaaatcatc aatcaattag atgtttctga tatgaccgtt
    21121 cgcagagatt tagatgaatt agaaaaatcg ggcaagctgc tgcgtgtcca tggaggggct
    21181 caaagtcttt cctacacttt agatcaagaa ctttctcaca ctgaaaaagc cactctgcag
    21241 attcaagaaa aaaaagaaat tgctgccaca gcagcctcct tgattcacga gggagaaacg
    21301 attttcttag gaccaggtac aacaatcgaa ctattggcga cgcatttact gaacaagaaa
    21361 attcggatca ttacaaataa ttatccagtt tttgatattc ttgctcaagc agatggacca
    21421 gaaattctac tgattggcgg tgatttccgg aaaaataccg gcgcatttgt cggaccgatc
    21481 accaacgaca atttaagaag actaaagttt acaaaagcat ttatcagttc aaatggcgtt
    21541 cacaacgaag cgatctctac ttacagtacc gaagaaggag aagcacagca gatcgctcta
    21601 aataattccc gtaagaaata tctactcgtt gataataaaa aattcaatcg agatgatttt
    21661 tatgtgtttt ataatttgca tgatttcgat ctgctcatta cggatcgcca gatctcgcgg
    21721 gaggttcaag agcactatgc acaatatgtc gatctgcaaa ttgctgattc aaccgaaaat
    21781 accactatag gagcgtaact atgaaaggga ttatatttga tttcaacgga acaatgtttt
    21841 tggactctca tctgcatgaa gctgcttggc tgcatatgat acatgatcat acaaaagatg
    21901 gtttatcaga cgaggatatt ttaaaaaata ttcatggtcg gacgaatacg gagattttga
    21961 aacatttcat ttcagagcat ttgaccgcct ctgagatcgc cgtattgtca gaagaaaaag
    22021 aaagctatta tcgtatgctt tgcttgcaaa atgaaaacga gcttcagttg acaaaagggc
    22081 ttgaagcgac tcttgaccag ctgactgacc aacagattcc attaacgatt gccacggcta
    22141 ctgtcaaaga gaatgttgct ttttattttg atattttcga tttagcccgc tggtttgact
    22201 gggacaagat tgtctatgac gacggctctt tccctgggaa accgcaaccg gatatttttc
    22261 ttaaagcggc agacaaactg agcttagctc cggctgattg tcttgttatc gaagatgctt
    22321 actcaggatt acttgctgcc aaacgtgcgg ggatcggaac aattattgca attgatccat
    22381 tcggtaaaaa caaaacagta tttgaacagg cacagctaaa taaagatgga accatcaaag
    22441 attttactgg tttttggaca gagcacctgt ctgccacatc cgttaattct taaacgattc
    22501 aactaactac caaagaaaag caactgaaat gcacacctcc ttttataaaa aactgccagt
    22561 gaaacgacat gaaagataca ctgctatatc cttagtgaaa tatagcagtg tatctttgac
    22621 tttttcattc ttttaaccat atgacgcgtt catctctatc tataattaac attaaaaata
    22681 gtgttttcac attatttctc aaaccaatct taaaaatcat caggagtact tttttgatat
    22741 acttttatta atgctgggcg taatggttaa aagatttcca ctagttcctc tccaccaagc
    22801 tgagttgaaa aatgcttatc tgtaaacaac atagacaaag aggaaataaa cgtttccgta
    22861 tctttctctg tcccaaggat attaattaat tttcctacta ggtaagtatc attaaactta
    22921 agatctgcct tcacatcaaa agttttgata aaagctttta tcatgcgtac aattttgtct
    22981 ttatcgccat aattgatttc accactcata tcaatatagt cactaatccc tgaagaaata
    23041 atggcaactg cttcttcttt cgccttatca gataattcat ttaatgactg ggattgattg
    23101 atgacttctt gagttttatc tgttacgtaa tctctcatta tcctatcggc atactccgca
    23161 aagtaatctt gaaaaaattc ttctacaggg taaccaactt cgttttccca accaggagaa
    23221 atttcattat cagtacttat taaatctatt aaaaaccaac ccgttctctg agtgttgatt
    23281 ctataatatt tttaatttcc tatataaatc aaaaatatta tagaatcaat aagctaatta
    23341 agaaacaaac tttagaaact ctagaagaac ttctgaattt atatagaagt gtgtagctac
    23401 ttactaaata tacaaaagtt aaaataaaat actctggata ctctttatac tgcattaaag
    23461 ttagtaatat taacagcaaa gatatcgaaa aaacaataac ataatatgtg tcaaaatttt
    23521 tcataataag aactccctta ctgttgcata ctaacataat ccaaaaactc ttctacttca
    23581 gctaaagaca ttccactttc tttagtccaa aaaacagttt tttctatcga tccttctctg
    23641 taaactgtaa atgtcggagt cgagttaatt gagtagcgac tcttgaattc tttccactca
    23701 ttttcatctt ctctaacttt tgctacattc acgtaataaa tattttcttt tcccttttct
    23761 accagcatat cgaatagctt cctgcttttg atgatcgatt tcttgtgcta catctagtaa
    23821 attttcatca atttctatat cataaagtat ttcactagtg tctacatcca caatatatac
    23881 tttttatcag tagcaatttt tgagtaatcc gttaaaatga tctcagtctc gtcactttca
    23941 tcagtataaa tatacgttaa caatgaactc accttttcaa tcggtgttag ctctaagcct
    24001 actgtatttt ttactattga gtcattattt accaccataa cttcgtactg tatataccat
    24061 cctatttggt ttccatcaat atcagtaatt ggctcactag ctaatggagt ctccggaaca
    24121 atattttcct gatatttctt aaactcttct gtagcacgaa aaaggttatc atgttcctca
    24181 cttgaaactg gttgagtcat agaaaatgtt acttggctaa acagagggaa aataaacact
    24241 acaatcaaca aagtataaaa aaaagccctt ctttttcatt ttttatcttc caatcacaaa
    24301 ttgaaaaact tattcctgca actttgagtc tagtatcaca atcgactgac tacaactatt
    24361 catttacatt ctactttaag ttcctcctta accattaatt ttattaaaga tacttgtaat
    24421 agtgattctc acaaaatttc atgaaaaata ctttattact gtatctaaaa ttccaataca
    24481 caacttatcg actattatct gaaaaccaaa acttattatt ccttgatgtt attctagcaa
    24541 tagtaactgc ttttttattt ttttgcatat tttttcggcg ttacttttta aagttaactt
    24601 tttcgacaaa tagaaagaga tacgaatcac tgattttttt gaagaaaaga aatggagtga
    24661 gctgaacgtt agagatattg ggagctttcg taactgctta ttaaacgata atggttattt
    24721 gcaaccgtat tgctcgcttc tacatgaaat atttcatgaa atatttcgca aaacctttga
    24781 ttatgtagtt acgtttccat tcttgactaa aaatgtttca aaaacgaaca aaggtgttaa
    24841 aaggtaaagt gatcaatact actggtcaaa ataagaggtt gaaagggaat tgaaaaataa
    24901 ctacagataa tacttttata aacgtacgtg ctttgtggtt ctgtagttaa ctatataact
    24961 ggaattaggg aatggtatca atttgaaaag aaagattcta ccaatttatc atatgttaaa
    25021 aatggaaaat caacagcagt tctttcggaa accttacaca aaaaccgatt attgaaaata
    25081 gccgaaaaac gtttttcggc tattttttta attaagtttc cttcttctta ccttttccca
    25141 ctattccgtt tcacatcttt gatgatcttc agcgcacgtt ttcgtgtttt gatatttggg
    25201 cttttcaagc ggcggtatgc acttgagata tctgtttttt ccatatggac gacccacttt
    25261 cttctttgat tgtagtcatg ctaaaaaaca caaatcgaaa caattacgat ttgtgtttta
    25321 aatggtatca aaagttaaaa tgaaagtcaa cttgttaaaa cagtcgatca gactagctga
    25381 ttgactccta tctctggaaa taaattttca ttattacgtt ttagctacct aaaaaaggcc
    25441 ctcttgtccg ttaactttaa tatctattgt tatagctagt ttacctattg aacgtactgt
    25501 gtgttcgata gaacact
//
LOCUS       DBNICI010000023        25464 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.023, whole genome shotgun sequence.
ACCESSION   DBNICI010000023 DBNICI010000000
VERSION     DBNICI010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 25464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.023"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(140..1093)
                     /locus_tag="AC1ZCC_000767"
     CDS             complement(140..1093)
                     /locus_tag="AC1ZCC_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HIQ1629039.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1116..1871)
                     /locus_tag="AC1ZCC_000768"
     CDS             complement(1116..1871)
                     /locus_tag="AC1ZCC_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1629040.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(1868..2827)
                     /locus_tag="AC1ZCC_000769"
     CDS             complement(1868..2827)
                     /locus_tag="AC1ZCC_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HIQ1629041.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(2820..3770)
                     /locus_tag="AC1ZCC_000770"
     CDS             complement(2820..3770)
                     /locus_tag="AC1ZCC_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1629042.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            3920..4372
                     /locus_tag="AC1ZCC_000771"
     CDS             3920..4372
                     /locus_tag="AC1ZCC_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HIQ1629043.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(4461..6728)
                     /gene="gshAB"
                     /locus_tag="AC1ZCC_000772"
     CDS             complement(4461..6728)
                     /gene="gshAB"
                     /locus_tag="AC1ZCC_000772"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HIQ1629044.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(6915..7913)
                     /gene="ftsY"
                     /locus_tag="AC1ZCC_000773"
     CDS             complement(6915..7913)
                     /gene="ftsY"
                     /locus_tag="AC1ZCC_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIQ1629045.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(7924..8733)
                     /locus_tag="AC1ZCC_000774"
     CDS             complement(7924..8733)
                     /locus_tag="AC1ZCC_000774"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIQ1629046.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(8742..12323)
                     /gene="smc"
                     /locus_tag="AC1ZCC_000775"
     CDS             complement(8742..12323)
                     /gene="smc"
                     /locus_tag="AC1ZCC_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIQ1629047.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(12343..13029)
                     /gene="rnc"
                     /locus_tag="AC1ZCC_000776"
     CDS             complement(12343..13029)
                     /gene="rnc"
                     /locus_tag="AC1ZCC_000776"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIQ1629048.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(13377..15158)
                     /locus_tag="AC1ZCC_000777"
     CDS             complement(13377..15158)
                     /locus_tag="AC1ZCC_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HIQ1629049.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15184..16098)
                     /locus_tag="AC1ZCC_000778"
     CDS             complement(15184..16098)
                     /locus_tag="AC1ZCC_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1629050.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16125..17087)
                     /gene="opp4B"
                     /locus_tag="AC1ZCC_000779"
     CDS             complement(16125..17087)
                     /gene="opp4B"
                     /locus_tag="AC1ZCC_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HIQ1629051.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17087..18031)
                     /locus_tag="AC1ZCC_000780"
     CDS             complement(17087..18031)
                     /locus_tag="AC1ZCC_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1629052.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18032..19036)
                     /locus_tag="AC1ZCC_000781"
     CDS             complement(18032..19036)
                     /locus_tag="AC1ZCC_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1629053.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(19304..19546)
                     /gene="acpP"
                     /locus_tag="AC1ZCC_000782"
     CDS             complement(19304..19546)
                     /gene="acpP"
                     /locus_tag="AC1ZCC_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIQ1629054.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(19668..20669)
                     /gene="plsX"
                     /locus_tag="AC1ZCC_000783"
     CDS             complement(19668..20669)
                     /gene="plsX"
                     /locus_tag="AC1ZCC_000783"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HIQ1629055.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(20718..22754)
                     /gene="recG"
                     /locus_tag="AC1ZCC_000784"
     CDS             complement(20718..22754)
                     /gene="recG"
                     /locus_tag="AC1ZCC_000784"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIQ1629056.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(22871..24553)
                     /locus_tag="AC1ZCC_000785"
     CDS             complement(22871..24553)
                     /locus_tag="AC1ZCC_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HIQ1629057.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(24605..24967)
                     /locus_tag="AC1ZCC_000786"
     CDS             complement(24605..24967)
                     /locus_tag="AC1ZCC_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIQ1629058.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            25237..25425
                     /gene="rpmB"
                     /locus_tag="AC1ZCC_000787"
     CDS             25237..25425
                     /gene="rpmB"
                     /locus_tag="AC1ZCC_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIQ1629059.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
BASE COUNT         6936 a         5591 c         4491 g         8446 t
ORIGIN      
        1 taaaaaccat ccgtaaaatc atcaatacga ttttacggat ggtttttgat aacttctttg
       61 aggataggct cttgctattt aagaatgtaa gaaacattat tgttacatag tatgagagtt
      121 taatcctctg ttatttatgc tatttaaatg cttgattcac atcttcgatc atcagtttca
      181 ttgattctaa accgcccccg cttaaatacc agacatctgg ttcaagggag atgatctgct
      241 gatttttacc agcattcgtt tgtgctacca gttcattggc agaaatatca tctttgctgt
      301 cgtctccacc aatagctttt gttctgtcta ctacaaataa gatatccgga tttttttcca
      361 gcacatattc ataagaaaca ctttgaccgt gagtggatgc ttcgatttga tcatctgctt
      421 gttcaaagcc aaataaatca tggatgaaac cgaatctaga accagatccg taggctgata
      481 attgcccttc attcacaagt gtgactagtg cttttttgtc agtagcttct gctttctttt
      541 tcgtctgatc gattgcacta gataattcct ctaattgagt tttagcttct tcttgcttac
      601 caaagatctc ccctaaagca gtgacatttt gctggaaaga ttcccatgga tttttcgcat
      661 ctaaagaaag gaaaatggtc ggagcaattt gacttagatc tttttgaaaa tctctttgtc
      721 ggccagaaat gatgattaga tctggctgaa gctgattaat tttttctaag tctggttctt
      781 ttattccacc tgctgattct acttttttat aggatgaaag atactcagga aggctgcttg
      841 tagctgctcc aactacctta tctccaacac caagagcatc catcgtatct aaagaaccat
      901 tgtcgaatac gacgactttt tctggattat ttggcacctc gatttggtct ccattactat
      961 ctgttatcgt caatgtagtt tcagtttgtg tgctgctttt ttttgtgtct gcctgactgg
     1021 tgttgttgct atttccacat gctgctaaac caaatgatgc tagaatcaag atacctacta
     1081 ctaatttttt catctcttct ctcctaatgt tagttttaga aataaagaca gaatctgcgg
     1141 ccttcaatct cacaaatccg tatgtccatt tcgtataatt catccaatac atcttttttt
     1201 atcatttctt gggtcgttcc ttgcgtaaat aactgtccat ctttcattgc aacaatttcg
     1261 tctgcataac ttgcagcaaa gttgatatca tgaagaacaa taacaattgt tttcccgatt
     1321 tcttgtacta aattttttaa aatctgcatg atttgaaccg caaaattcat atctagattg
     1381 ttcaaaggtt catccagtaa aatataatcc gtgtcttgcg ctagcaccat tgcaatatac
     1441 gcacgttgga gttgaccgcc tgaaagagtg tcaatcgatt gatccgctat ttcttctaac
     1501 ccaaggtgtt ccagtgattc ctgaacgatt tcctcgtctt ttgtcgtcaa ttttcctttc
     1561 gtataaggaa atcgtccaaa ttctactaat tctttcacgg tgatttggac atttatttca
     1621 ttcgattgtc gtaggatcga taattttttg gctaattcat tggatttcca agcctttact
     1681 tcgttatggt ctaaatagat ttcacctgta tcttttggta tcaaacgact gatcatcgac
     1741 aacaccgttg actttccagc accatttgga ccgataaaag cagtcaactt cttttcagga
     1801 atcgttaact gtacatcttt aaccactgat tttccatggt acagtttatt gaccttttgt
     1861 aaatcaatca taggatactc ttcctttcgc gaatcaattt tgtgacaaaa taaattcctc
     1921 cgacaaattc aacgacgaca ctcactgttg tattccagcc gaaaatctgc tctactgcga
     1981 gctgtcctcc tgtcagaaac agcatagcta ccagaaagct tccgataaat agctgaccat
     2041 gacgataagt agaaaagaca cgataactga tatttgcgac aataaaccct aaaaaagtaa
     2101 ctggacccac aagagccgtt gatgtccctg tcaaaagact gacacaacat aaaatcaaaa
     2161 attcaaaacg atctgtgtct atcccgaggt ttactgcttg ttcatttccg agatggaaaa
     2221 cgtctagcac atgtcgatat cgaaataaca ggtaccagca gccgataatg atcaaagaag
     2281 caggcaaaag atactgactc tggatattgc caaagcttgc aaataatctg ccttgaagca
     2341 aatcatattc attcggatcc atgatgactt gtaaaaaggt actgacacta ccaaaaagcg
     2401 tacctaaaat catccctgac atcagtaata aaaaaagatt cgctctcatt tttttcaaaa
     2461 gaaagaaaaa taacaataga ctcagcccga ccatcagccc aacgttcaat aagaaaaaaa
     2521 ctgtacctgt ctgctgaagc atcgtcactc ctccgataaa gaagaaaagg accgtttgga
     2581 caaggacata taacgactca agtcccaata ctgaaggagt caaaaactgg ttatgcgtaa
     2641 ttgtctgaaa gctgattgtg gcactgctga cagctagcga gacaaggata aatgcaaaaa
     2701 ttttcttact cctcaatgca catgcaaaat gccagtttcc atatgtatgg atcgtcaagt
     2761 acattcctgt aacaaataaa acaagaacaa gcataccaat aaaaatagat ggcttttttt
     2821 taagcatatc gtcgtcccct tatcagaaga taaaggaaga tagccccacc gatgattcca
     2881 acgatcacac tgactggtat ttcataagga aaaatcaaca cgcgagcgac aagatcgcaa
     2941 aatactaaga acaaactacc tgctaaagca gttacgccaa ttgttttgtg cacttgatct
     3001 ccatatattc tagagacaag attcggaatg atcactccga taaaaggaat gcttcctacc
     3061 atgatcaaca tgactgcact agccaatgat actaagccaa tgcctaaaaa ctgcatcttc
     3121 tggtaatcta ttcccagatt tactgcaaac gattcgccca gaccaatgat cgtaaatcgg
     3181 taagcaaata gccaaaccaa gagcaacaat ggaacaacag catacaataa ctcataattt
     3241 cctcgtatca cagtcgcaaa attcccttga agccatgaag tcatattttg taccaactgg
     3301 aattgataag caaaaaaagt agcaactgaa ccaatgatat tccctaacat gactccaatc
     3361 aacggaagca tcaattgatt tttctggagg aacaatcgcg taagggataa aaaaagcaat
     3421 gttcctgcaa aagcaaagca aaatgctgtc aaggatcgga caatcaagga tgcaccaggt
     3481 aggaaaatca tagccactaa aatacctaaa cgcgcactat ccatcgtgcc cgcagtcgtt
     3541 ggtgaaacaa atttattttg ggtcaaatgt tgcatgatca acccacaaat actactggtc
     3601 gctcccacaa ggatcaaaca aatcgttcgt ggcaaacgag tcactagcaa gatttgtaag
     3661 ctttgcccat ctaactggag cagttctgac aattgtacat tcgatactcc gatgaacaat
     3721 gaaatactac ttaaaataac taataatgct actagtcgtc cgtagcgcat ccaatccacc
     3781 tttctaattg agaatgattt tcattcttat tttaagtgaa tcgttgaaca aaagcaaccg
     3841 ctttttcata tgttttaggt attattggga atatgctata atcgtaagaa agacaaagag
     3901 aagaaggagg gtatttttta tggcacaaga tgatgtaaaa agccatttgg ataagggact
     3961 ttatggtaca cctttagtga accctgaaga acagcataaa tatttaggga cattcaggga
     4021 acgctgctat cttagtatga ctgttgctga aatgatccaa ccaaaaaaca aagaaaactt
     4081 tttaaaagaa ctcaaaaaac atccagatgc ttccatactc ttgaatgggg ctctacccct
     4141 ttctgtccaa aaccaatata tccagctagc aacgaaaaac aatagccgtt tcactgtagt
     4201 caacgattac gtcactgcaa atcctgagga attcggattg ctcttaacag caaaagaagc
     4261 agtaaatgaa cctgtgattg atatcgaaaa aaaataccca gaagaaaact cttcgccagc
     4321 tgaaaaagaa aaaccaaaaa agaaaaaaag cttctgggat cgtttatttt aaaacgaaaa
     4381 aacacataaa tttttacatg caaaaaaagc ttttgatcag attgacatca aataggacaa
     4441 tctgtcaaaa gctttttctt ttaaaatact tcaggatata acaatttcaa tacattcatc
     4501 gtcaatcttc tgccttttcc tgcaaaagga taaacatgca tatgcatggc tggattgaaa
     4561 tttgcttcga tgatcccata tgctttttta tcagttgtag gatcgatttc tttatctggt
     4621 ataatcaaat cgataccact aatcttagct cctagtgctt ctaccgcaga aacggcgatt
     4681 tttttgtaag cttcagaaaa ttcatcagtc atatcgatcg aatcaccacc tgtactgata
     4741 tttgagttct cccttagata aacaatttga ttggcttggg gaactgattc gatcgtcaac
     4801 ccttgttctt tcaacatcaa ttgttctaat tcacctaatt ggatcaattc taaaggtgca
     4861 cgatgattcg ttccacgcaa agggtcacta tttttttctt caacaagttc tttcactgtt
     4921 cggatactgt caccaataac attagcaggg actcgcaaca tgatggcttg gacttctccg
     4981 tcgatcacaa agaaacgata ttccgttccc ggcataaatt cttctactaa aaccgaactg
     5041 tcttcacgaa aagcaatggc taaacctgct tgataatctt ccagactagc tccttccttg
     5101 aaaatcgtga tacctaaccc gtaattggtt gatttaggtt tgatcacaaa tgcctgctcg
     5161 gcaaatctcg gataagcttt taccgcttct tccatggatg aaaattctgc gcctcctggt
     5221 acccggaacc cagcttcttt taagactttt ttcgttactg ttttgttttc catgataagt
     5281 gggacgatat agctgtcctt acttgtcatg tttgcatttt tcacatattc tacatggtct
     5341 tggaattgca gacgcaaaaa ttgatccgac tcatctaata ctttgacttg gacacctttt
     5401 tggatggcat cgaacataaa tatctgagta gaaagttcca tttcgcgaaa accggccaac
     5461 tgatagggac gttcatggct ttctttataa tattgatgtc ccaatgccac agcaacttct
     5521 tgctgactcg atagctgtgc ttcttgatag agtttcgctg ctaacgtttg ttcaggatga
     5581 tccatttgag tcaatgcttc ctctactaaa taaagagaat ccaaccaatc caattcaacc
     5641 agcatttgct tcattccttc aagaatttct ctaccttctt gatgaaattc tgtttgatct
     5701 gatggttgtt ctagtgctac tttgttattt cgagtcgtac cttctgctac ccattggtca
     5761 cagtcttctt tttcttctgt ccataacatg tacatcagga ataattgtaa gaaccgaact
     5821 tgctctggat tgatacccaa tcttgcataa ggattcaaat cgatattccg caattcaata
     5881 taacgaacac ctgttgttgc taaatctgcg actttctttc cgcctcggaa acgaagcggt
     5941 gtataaaatt ctttctcctc cgaaagtttc ccttcttcga tcattcgctc gatatctgct
     6001 aaatactgtt tcatagaagc ataggaaact ttcacatttg gatgattcgt atagcccagc
     6061 gcactgttgc gaatactgcg aacaggttct tgcggatgtg attcatctaa aaagtagttc
     6121 ttctcactca ttggtgaagc accgaataaa taagtgatca tccagcgata gcgcataaag
     6181 tttctcgctg ttttcaaata aagttctgtt ttgaattcgg aaaaatcttg aaattcttcc
     6241 tgatggctga acaatgttct tagcaactca tcgccaaatt caaaattgaa atgaataccg
     6301 cttaccatct gttttcgctt cccgtatttt tttgctaaat aacgtcgata caagacatct
     6361 tcgaaatttt ttaattttgc aataatgatc tcttcgtctt tttccggtaa ggcaggtggc
     6421 atacttaatg gccagatcat ctcttctttc ggtatagaac gagcagtcac atcataaaca
     6481 gcagcgagat actgaaataa ttcgggaata gaatcggtaa caggggtgat catctctgtt
     6541 tgtgtttcgc taaaatctgt ttggatatag gggtgatagg atcgatcgcc aaagattgct
     6601 ggatgatcgg tttttgctag atttcctgca agatcaaccc gctgtccttc ccgttctata
     6661 ccaaatcgag cttggtcgat aaaagggcgc gcattgacat gcaataataa ttgcttaaaa
     6721 ttcatcatta agtgacctcc aattcatatg aatctaatta taatcaaaaa gtctaagaaa
     6781 aagaacgaaa ttgtttgaga accgcttaca aataagtata aaaatgaagg catcaaaaac
     6841 acagctatgt taaaaacaat cattcaaaca aggagctgca acttttgtca cagctcctgc
     6901 tttttagaca tgggttattc ttcttttagt aatcctttga acaatccaac gacaaaatca
     6961 tttggatcaa acggctccaa gtcatcgatt ccttcaccaa gaccgaccaa tttcactggt
     7021 aaatgcaatt cattacggat agcaagtacg ataccgccct ttgctgtacc atctaatttt
     7081 gtcaaaacaa gaccggtcac atctgttgtt tctttgaatt gttttgcttg aaccatcgcg
     7141 ttttgaccag tggttgcatc taatacaaga agtgtttctt ggggtgcttc tggtaattcc
     7201 cgctggatta ctcgttttat tttttctaat tcgttcatca agttcacttt attttgcagt
     7261 cggcctgcag tatcaactag taaaacatct gcctgctctg tctttgctct ttccaaagca
     7321 tcgaatacga cagctgctgg atcgcctcct gcattgccac gaacaacttc aacaccagcg
     7381 cgttcgcccc agacaactaa ctggtcgatt gctccagcgc ggaaggtatc tgctgctgcc
     7441 atcaatactt ttttaccttc gtttttgaac tgatgggcaa gtttaccaat acttgttgtt
     7501 ttccctacac cattaacacc gacaaacaaa ataactgtta gtccgtcttc ttggatattt
     7561 agttcattta cttcattgat tccttccgct tcatagagat ccacaagttt ctcgatgatc
     7621 gcattttgga cttctgccga ttttttcgca tttctgagtt tcacttcttg acgtaatgct
     7681 tcggtgattc gtatcgctgt ttcaaaacct acatctgcac cgataagtgt ttcttctaat
     7741 tcttcaaaaa agtcttcatc gactgttcga aaattggcaa acaactcatt catccgttca
     7801 ccaaatgttt tacgcgtctt ttctaaaccc ttgtcgtact tctcctgtac ttcaacttct
     7861 tctgttttct cttcgccagt aaaagcacgc ttgattttat caaaaaatcc catttgactc
     7921 cccctactct aatacaaatt tttggatcac tttggctacg ccattttctt cattgctggc
     7981 tgtcacgaaa tctgctgctt ctttgacgaa aggcacagca ttctccatcg ctacgcctag
     8041 tccagcatat tggatcatcg gtaaatcatt ttcctcatca ccgatcgcca tgatttcttc
     8101 ttgtttcaaa ccaagatctt ttgccaataa agaaatacca taagcttttg taacaccttt
     8161 tggcataaat tccaaaagca tacttcttgt tttgatgatc tcatacattt catggaattc
     8221 tgctggtatt tctttgattt taggatctaa ttcttcttgg tcataagcga ttactgcctt
     8281 attaagcaca aattcatctg taatatcgct tatatccgct ggttggaatt gcagcagctt
     8341 attcaacaca ttgtacaagg attgcttcct tggggcacta ggcagctgta aaaccacatt
     8401 gtcagacaaa acatctagtg gcagatccaa ttgctgagcc agtttgtata agcgttggat
     8461 atctgatact tccatcactt tcttttccat gatttcacca gtatcatttt tttgtaccaa
     8521 accgccgttg aaagtgatgc tgtagtcacc ttcatctacc agtcctaatt cttgcagata
     8581 atgcgccatc gcagctaagg ggcgtcctgt acatagaacg atcttgaccc cttgctgttt
     8641 tgcttgtgct aatgcttgtt tattctcatc tgaaatcagc ttttgttcat ttaataatgt
     8701 accgtccaaa tcgattgcta ctaatttgat cattttcgtt cctacacttt cgtttcgttg
     8761 attttcgcct cttcattgat atcttccatc cgtacagaaa cgatttttga tacaccggat
     8821 tcctgcatcg tcactccgta cagtacatct gctgcgacca tcgttccttt gcggtgtgtg
     8881 actacgataa attgtgtgtc atcttggaaa tcgctcaagt aacgaccaaa acgtttcacg
     8941 ttggcttcat ctaacgctgc ttctacctca tctaagatac aaaacggaac aggacagaca
     9001 cggataatgg aaaagagcaa cgcgatggct gtaagtgctc gttctcctcc agaaagcaaa
     9061 cttagacttt gcagtttttt gccaggaggt tgtacttcaa tctcaatacc tgttttcagc
     9121 aaatcagttg gttccgtcag tactaattca gctttaccgc cgccaaacat atttggaaag
     9181 acaattttga attcttgacg gatagcttca aatacttctt tgaatcttgt ccgcacttca
     9241 tcatccatct catccatcgt ttcaaacaat tggtttttcg ctgctaacag atcatctcgc
     9301 tgtgttgcta agaatgtatg tctttcactg acttgttcgt attgttcgat tgaatttaag
     9361 tttactggtc ccagtttttc aatctgttct tttaaggatg aaacctttgt tctactgtct
     9421 tcgatatcgg ttgtttcttg atagtcttgg gaagctttct caaatgtcag ctgatattct
     9481 gactgcagat aggtcaaatg attatctaaa atcatttcag ctcggtcttt ttggacttct
     9541 aatttggatt gttccgacag tcgtgctttt tgctcacgat tacgctctgc caagactgct
     9601 tcaagcttat cgatctcctt ttgccttcta tctcgctgct ctttggcttt tgccaactcg
     9661 gcttttaatg tttctcgctg agcagataat tcgttaattt gtttttccaa actttcttct
     9721 gtgacttcat gatcagaaaa gtctgctgtc aatgtcgcca actgcttttc tatcgcttct
     9781 tgacgttcgg ttgcctctgc tttttggact cgggcaccgc ggagctggat ctgcagatga
     9841 ttaaactgct ctttcaagac agcaagatct gcttgttctt gtgctttttg actttgtact
     9901 tgtgctcttc tggcttccat ctggtcgctt tcttgactta acgatttgat ttcttcgtcg
     9961 attttttgtc gttgcgattc aagatctgtt tgtttgtctt tcaattcgtt cgtttgcttt
    10021 tcgtaatctt cgataaactg ctgcagttca cgtgtctcaa aagttgagat ttgcttttct
    10081 ttttcaaaac gttcaagttc atttatgatg ttttgtagct gatttgtcgc ttcttgttct
    10141 tcaaaacgta attgttcgcc tcgtgtacgc aaaacttctt gttcttcggc taaacgagct
    10201 gtttcttgtt gcagctcctg aacttttttt tcttgtgctt ggagttgttt atctgcttct
    10261 tcaaattcac tagtcaactg tttcaattct tgattttgta caaacaaact gccttggttt
    10321 cctcgtttat tcgctccacc tgtcatggat cctccggcat tcatcacatc accttctaag
    10381 gaaaccactc gatattgata gcggatcgtt tgcgcgattg cattggcact agttaagtct
    10441 ttggctaaaa ggatcgttcc tagcaggttt tgaaccacag tttggatttg atcgggataa
    10501 ctgacttgtt cactggcaat ccctataaac ccttctacag ctgctgcttg tgtcaatata
    10561 tgagccggta attgtcttgg tttgattgtc gtcagtggaa ggaatgttgc tcttcctcct
    10621 ctttgctgtt tcaaatacgt gatggcttgc cgagcatctt tttcattttc aacgatgaca
    10681 tgctgggctg ccccacctaa agctgcttcg atagctaacg tgaaatctgc aggaacatcg
    10741 atcagttcag ccactgctcc gacaataccc gaaagctgct gtttatgctg caagaccaat
    10801 cgaacccctt ggtaaaaacc aaaatagttt tcttggattt cttgaaggct tttcttacga
    10861 gcacgtagct gctggacttg attcattaat tgatacattt taggctgttc ttttgctaat
    10921 gcttcttgga actttttccc gttttgttgg atcatttcta acttctcttt tgtttcagtt
    10981 aaagatgatt ttagctgcgc ctgcttttgt gtcaattctt ctttttgtaa gatcaatcgg
    11041 tctactgatg cttctacttc cgattgcttg gctaacgtct gcttgctttt tgctgtttct
    11101 tgtatatatt gacgttctag atacttcagt tcgtttccta cagctgcttg ttcttgcatc
    11161 aaatcaacat attgatctct taattctgcc aataattctt tcgttgattt ttggtatttt
    11221 tctaattctt gctgtgtttt tatgagattt gcttcagctt tttggatctc tgtttctttt
    11281 tctgctgctg ctttcatcaa gctttcttga agcttttcaa aatgtttaac ctttttttgt
    11341 gcttctgcta atgaagtttg atattcttgc gagctttttt gtgtatgctt tgtccgctcc
    11401 tgcaaaacat ctttttgtcc ttctgtctgc ttcagttttt ccgaaagatc taataatact
    11461 tgctgatttt tttcgatcag acgatcggct tgcgcatttt ctttacgctg cttcgccaaa
    11521 atggattctt gttcttggat cgactcagat agtttgccta attctagatt gaatttcgca
    11581 agctgtgctt gtttgttgtc ccaatctttt tttgctgtct tgatttcagc aaccatcaaa
    11641 gagacatcgg tttgagtcag tgtttctttt agccgtaaaa actcctttgc tgcctcactc
    11701 tgggcggcca aaggagttaa ctgttcttcc aattcgtgga tgatgtcttg gacgcggctg
    11761 aggttgtctt ctgtttcaaa aagcttctgt tctgctttct ttttccgctg cttgtatttc
    11821 aagacaccag ctgcttcttc aaagatccca cgacggtctt ccggtttact actgaaaatc
    11881 gcttctactt ttccttgaga aatgattgag aacgattctt ttcctaatcc agaatctaag
    11941 aacaattctt ggatatcttt taatctgcag ctttgtttat tgatgaaaaa ctcgctttcc
    12001 ccagtccgtc gataacggcg ggttacactg atctcattgt actccaatgg cagataatga
    12061 tcactattgt ctaaaatcac tgtgacttct gcaatattca gctgcttacg ggtatcggaa
    12121 ccggcaaaga tgatatccgg catttttccg cctcgcagac ttttagcaga ttgttctcct
    12181 aatacccagc ggatagcctc tgtaatattg ctttttccgc tgccatttgg tccaaccact
    12241 gctgtgacgc tattttcaaa atcaatgact gtcttgtctg caaatgattt aaaaccggct
    12301 atttcaattc gtttcaaata caaagctttc cctcacttac ttttacagtg ctgccaatgc
    12361 attttccgcc gcagcttgtt ctgctaattt tttcgatttc ccttgtccag tgccaatcaa
    12421 ttgatcatct acataaactt cgatccaaaa tactcgttca tgagctggac cttcttcatt
    12481 gatcaatcgg tattcgattg atacatcacc tgatttttgc aagacttctt gtagttttgt
    12541 tttatgatcc atctcatgtg aaaaagcacc tgctttgact ttaggaaaaa tcactttttc
    12601 gatgaatgca tgggtcgtgt cgaatccctg gtcaagatat aacgcgccta aaaaagcttc
    12661 gaataggtcg caaagcaatg ctggtcgcgt acgtcctcct gaattctctt ctcccttgcc
    12721 caacaaaaca tattgatcaa agtgacattc tttggcaaat ttcgacaagc tgtcttcgcg
    12781 aacgatcgca gctcgtgttt ttgtcaattt tccttctggc aaatgaggat ataaacggaa
    12841 taaatactga gaaaccatca gttctaaaac ggcatctcct aaaaattcaa gtcgctcatt
    12901 atccgataat tgcagattgc gatgctcatt cacataggat gaatgggtaa aagcttgttc
    12961 aagcaaattt agatcgtgaa aaacgatgtt atagttttct tttagttctt ttgtcagctg
    13021 gttgtccatt ttccacattc cttttatgga tattttttcg atacttcctt attatactgg
    13081 ttttttgtca aatttgaaat ggttcggtca aaaggtaagt aaagctttaa taatcctagt
    13141 gattgacgag aagttgtgaa aaattcgttt agtttcgagt atgaagaaaa aattttgtga
    13201 aaatggcttt ccatattttt gcataaattt gacttaatac cgagaaactg gactttgaac
    13261 accgtttatt ggagaagtca tgaaatcagc gccttgacct gaatattaga gaaataaaca
    13321 gaaaaatatc aaaaaaccga aaaaggacat cgcccttttt cggtcatttt tagtgattat
    13381 gaacgtgaat ctgctgtaac agaaacattt gcccatgggt ttggtacatc ataagaccaa
    13441 tcccagtctt tgacacggtc gttgactggc aatacttcat tacggaacaa cgttggaatc
    13501 gcaaatgctt cttcaaatgc atattgttgc catttttgat atgcttcttt ttggtactct
    13561 tcatcaaatg ctttgcttga actgatatcg ttgatcaatt ttgtattttc ttctgattca
    13621 taacgagtgt agttgaacgc agcatttggt ccccatagtc cacttggatt tggatcagtt
    13681 cctgtattcc aagcagcttg gtaaacatca atttcaggat catcgttttc taatttatca
    13741 tagaaagaat tgaattcgat cagacgacct gttgtgtatt ggacatccaa accaatttct
    13801 ttccattgtt ggatatagta atctgccaat ggttgcgctg tttccccacc ggacatcgaa
    13861 gcaaagttga ttgttagttt atttccatct ttgtcttctc ttaaaccatc gtcatccaca
    13921 tctttgtagc cagcatcatc taatagtttt ttcgctttgt ctagatcttg tgtgaatcct
    13981 ttcatctcat catcatggta tgactggaat actggaggaa tcaatgaagt cgcattgctt
    14041 ctcaaacctg catagaaacg ttcacctact gcattattat ctaatgcata gcccattgct
    14101 tgacgtaatg atttatcagc catttttgaa tctggattgt attccacact ctttgtatct
    14161 gcattccatt tacctaactt gaatcctaga taagtataag aagtttgttc acggcctaag
    14221 tttgtatagc cagcaatttc tttataggta tcgtaagtat ctgttggcat ttggaaaatc
    14281 atatcatata atttagagtt caatgcttca gttgcacttg ccatcggtac agatttcatc
    14341 gtgatctttt tcaatttagg cgtaccattc cagtagtgct cgtttggcaa gtattctact
    14401 gattctcctg caacgatatt actcatgtaa taagggccaa aagtcactgg atttttacgt
    14461 acttgatcgc ttgattccat atctttgatt gctacacctt ctaacgtatg ttttggtgct
    14521 gcatatgacc agataccacc acccgattga agcatgcttg ggtttacttc tttatattcg
    14581 atttcaactg ttttgtcgtc tatttttttg attccggaga ttgtgtcggc acttccatca
    14641 tgatattctt ccatacctac gatgttacgg aaagtgtcgt cataacggat accagtgtaa
    14701 tctttattcc caatgatttc gtaagggaaa atcacatcgt ccgccgttac atcttcacca
    14761 tctgaccatt tgacgttgtc ttttactttg attgtcgctt ttttcgtatc ttcatctaga
    14821 tcaagcgttg cagctccgtc gtctgtgatt ctgaaatcgt catcgtttgc aaatagcggt
    14881 tcatgagaag gagccataaa agctgcatca tatgaatctt ggtaaaactc ccactggaac
    14941 aatcctttaa actgactgtc cgttgcgacc gcagcttcta attcgccgtc ttcgattgca
    15001 tcatcattat tttttgttgc aattggaaaa gttaattcct ctttgttatt gccgttgcct
    15061 ccagagttgt ttccagaggt atctgtacct ccgcccccgc atgctgcaag aacgacagca
    15121 gacatcagcg ctgcaaatcc aaaaatcgtt ttcttcattc ctatttcccc cttcgcttct
    15181 atactagcct aaccgttggc gcgcatctgc agaacgcttg aatgcttgtc caacataatt
    15241 gatactcaac atcaaaacta aaataaggat tgatgcgggt aaccagatcc attgccgatt
    15301 cactaagact tctccgttac ttgcataacc gatcaatgta cctaaactag gtacatttgc
    15361 cggcaagccg aaacctaaga aagtcaaagt tgtctcgata ccgatgtttg ccgcaaagtt
    15421 catcgtcaaa ttagtaatga ttaatgaact tagattcggc atgatttcgc ggaacatgat
    15481 tttaaaatca ttggttccca ttgtttttga ggcactgaca taatctcttc ttgcttcaga
    15541 taatgtcttg cttcggaata atcgggcttt agctacccaa taaaatgcac tcatgatgaa
    15601 tataaaggac cagacattgt atctggaaat aatcgatacg aagacgataa tgatcagcat
    15661 gatcggtaga atcatgataa aatcaacgat ccgcatcaag atgttgtcaa tgatccctcc
    15721 gtagtagcca gagatgatcc ctaatccaac cccgatgatc gaagtaagga tcgtgatcgc
    15781 aaatccgatc gtaacagagt ttctagcacc tatgatcaat tgtcctaata catcacgtcc
    15841 gccttcatct gctcccagaa tgaacttttg tccttgtgtc gaaactgttc ctggtgcagc
    15901 gtatttgtcg aagatactaa catacatcac tttgtcttga tcggtcagca tcgcaccgat
    15961 aaagatcact aataataaaa ccactagcaa acctaatgaa aaaatagcaa ttttatcttt
    16021 tttaaattct cgagcaatca ttcgtatccc cataggagga atactttctt gaactgctgt
    16081 attgtttttt tcactcatcg atccattctc ctttctgatt ggttttattg aatccgaata
    16141 cgcgggtcaa cgatactcat gatgatatct gaaagtaatg tccccactag cgtcgcgatt
    16201 cctaaaatca atactaagga tgtgatcacg gtatagtccc gttggccgat ggaatcaata
    16261 aatagttttc cgactcccgg ataaccaaaa atcttttcga taactacaga accagcaatc
    16321 aacgaagtga tttcataccc catttgagaa gccatcggca acgctgcatt tcggaaaata
    16381 tgtcgactga atactttatt cgtcggtacc cctttggaac gggctgttct gacaaaatcg
    16441 agcgatttcg aatcgattac ttcacttcgc agatattgga ttgtgacggc agtcgccaaa
    16501 aatgcttggg taattgatgg taacagaata tatttcaaac ggtctatcca ttgagtgata
    16561 aatccacctt gcaaacctag tgtttgtgat ccacttgttg ggaaccagcc taaacgataa
    16621 ccaaaaacaa atagcatgat caagccgaag ataaataaag gaacagcaaa actaaagaag
    16681 ttgtagatta cgacaatctt atccaagatt gaattttgat accggccagc aagcatcccc
    16741 agaggcacag caatcaaata agtaaggaca actgtcaaga tcgatagata taaagtgttg
    16801 atgatccgtt cgccgatcaa gtcagcaaca gagcgtttgt ataagaaact ttgaccaaaa
    16861 tcgccttgca aagcatttcc gatccaacgg aagtactgga cataccatgg gtcattcaag
    16921 ccagctgctt gtcgcatctg ttcgatcact gccggatcct gatttggatt gatcaaaccg
    16981 gtaaatggat cccccggcat cgcttgggct aataagaaga tcagaacact taaaataata
    17041 acttgcggga tcatcaataa aacacgtcgc agaatcgttt tccacattag cttgcacctc
    17101 catctttcaa cgccactttg tgtgtagttg tgatcgtacg caaatcgtag actcgaccag
    17161 tcgcatcata gtattctttt tggttttgga tatactcttg ttctactctt cgtcgatttt
    17221 ctttgtggct ttccctattt tctacatcga tttgcggaat agctgaaagc agacgtttcg
    17281 tatagatatg tcgcgggtca ttataaatgt cttctcttgt accgatttct acaaatcgac
    17341 ctttgtacat aattgcgata ttgtcgcaca tgtgttttac tacacctaaa tcatgtgaaa
    17401 tgaataaata gctgaggccg tattcttgct ggattcgttt catgaaattc aatacttgcg
    17461 cttgaacaga tagatccaga gcagatacag gttcatcggc tacgatcagt tttggattgg
    17521 tagcaacagc acgggctact cctagacgtt gacgttgtcc gcctgaaaat tcatgaggat
    17581 atttatatag cgcatcttcc ggcatcccaa cgatatctag cagtcctttt acctttttct
    17641 tttcttcttg atcgcttaac cgttcaaaat ttcggatggg ttcagcaatg atatccaaca
    17701 ctcgcttttt aggattcaaa ctggacattg aatcttggaa gatcatctgc acatccttgt
    17761 tataacccat ttttttgcgg ttgcttcgct tcgttacatc ttttccttca tacatgattt
    17821 gtccggctgt taccttttct aatcccacaa cggcttttcc cgtagtagat tttccagagc
    17881 cagactctcc cactaaacca tatgtctttc ctttttcgat aataaaatcc acaccatcta
    17941 cagcatatac atgatccgtt actcgattga agaatccact gcgaataggg taatgaactt
    18001 tcaggtcttt gattgtgatt aattctgtca tctacacctc tcctttttcg tctctaaagt
    18061 ggaaatgctt atagcaggag caacgtacca agtggttcgg tgcgacttca tgaaggatag
    18121 gctcttcttc atgatcgctt gctggtatcc atggaatacg tggtgcaaac cgacaacctt
    18181 tacgaggcat gtttttcaga gaaggaacga caccttcgat cacatggagt tctgcatcat
    18241 gatttccttc ttgtggtata gagttcagta aagagcgtgt ataaggatgc ttaggatgat
    18301 caaacagttc ttttacacct gctacttcaa cgaactgtcc gccatacatc actgccactc
    18361 gatcagccat ttctgctacg actcctaagt catgagtgat caagatgatc cctgtttttg
    18421 tttcttcctg aaggtctttc agtaaatcaa gaatctgtgc ttggatggtt acatctaggg
    18481 ctgtcgtcgg ttcatctgca atgacgattg gaggtttgca ggcgattgcg attgcgatga
    18541 tgacacgttg tctcataccg cctgatagtt catggggata ttggcgtcca actctcgcag
    18601 gattaggaat ccctacttgt tctagcaatt ctagtgcacg agcttgacgc tgttctgcat
    18661 tcatctttgt gtgataggaa agtccttctt tgatctggtc ttcgattctc atgagaggat
    18721 ttaatgcaga caacggatct tggaaaatca ttccgatatc atttccgcgg attttattat
    18781 agagcgtttc gttcaggtcg attaaattca gatcgttata taaaatctca cctgtaattt
    18841 ttgtgttatt aggatcaagt aatcccataa tcgtagtagc caaggtactt ttcccgcacc
    18901 cagattcccc aacgattgct aaaatctcgt ttttgtctaa agtaaatgaa acgtcatcta
    18961 ctgcatcgta atagtcatct ttgagtcgga atcctgtatg taggttctga acttctaata
    19021 attggttatc ggacaatcga atacccctct ctgcttccta aatggaagtt tttcttaata
    19081 aaaaaggaca ttacacagaa taaagcgttt tataacaaag gttattctta tctttgtgta
    19141 atgaattatt aataattata tagaaaatcg ctttactttg caacataatt tctgtttatc
    19201 gccagaatta tcagaaaaat gacttttacg tgtcaaaaag attaaaaaaa gaaggataaa
    19261 aagctgtatg cagcttttta tccttctttg cttttatttg aaattattta agatggctgc
    19321 tgatataatc tactgcgcca ccgactgttt caatttgttc agcgtcttca tctgaaattt
    19381 ccgtaccgaa ttcatcttca agctctaaaa cgaattccat aatactaatt gaatcagctt
    19441 ttaaatcatc tttgatgttc aattcatccg tcactttatc ggtatctatc tcaaaatgat
    19501 ttgaaataat tttcgctact ttgttaaata cttcttcgcg agtcaacttt ttcacctcca
    19561 agaatcatac acttgtcaat tacctacacc atggctaatt ttactggcat ttttcaggaa
    19621 tgtccacctt tttttcaatg gaacggattg cttcagatat ccaaatacta ttcttcgctt
    19681 ttttcaaatt gttcgactaa ttgtccaact acatctgtct caagcattgt gtggatttgg
    19741 cggatcgtat agcgaacagc atcggggcct gttgctccat gagttttaat gactggtgct
    19801 ttcaagccaa acaaaacggc accgccatgt ttggaataat ccatttctgc tttcaaagaa
    19861 cgtaagccgt cttttaaaag catagcgccc atttttcctt tgatcccttc atttagaata
    19921 gcagatttta atagattcat catattcatt gctgttcctt caatagattt caaaacagcg
    19981 ttgcctgtaa atccatctgt gaccactaca tcagccacac cattgagtag ctcacgagct
    20041 tctacgtttc caataaaatt caagcttgtt tcattctgaa ggagttcata ggcttttttc
    20101 gtcacttcac taccttttgt tgcttctgtt ccgttgttta agagtgcgac acgaggacgt
    20161 ccgatacctc ggactttacg tgcataaaaa gaacctaaaa taccgtattg caacaaatgt
    20221 tctggtttgt tttctgcgtt agcgccaaga tccagcatat cgaaaccggc accttctttc
    20281 cctactactg gcaaggtaga catcaagcca gggcgttcga tatttttgat acgtcctacg
    20341 atgaacaacc ctgctgctag tagcgcgcct gtattccctg ctgaaaatac agcatctgct
    20401 tcaccgtttt tcactgcttg tgcagcaagg accattgatg cagtcttttt tcgtcgaatt
    20461 gcttttactg gctcatcatc actattgatt ttttcgtctg tatggataat cgtgatgttt
    20521 ttttcatttg ttaagtattt cttgatttct gcttcttttc cgtaaagtaa gaattctact
    20581 tctggaaaat cttgctgcgc taaagccacg ccttctacaa ttgcttgagg tgcgtgatct
    20641 ccgcccattg catctacggc aattttcatt gtttccactc ctttttccgt catcagtata
    20701 aagtatagca cagtctgcta atcgaaataa cgattatcct gattttctat ttggaggctt
    20761 tgcgctaaag gctgatactc aggaagcatc tgccaatcct ttatctgcca aagggctgca
    20821 gcctcttctc tggctacctc caaaacattt ccatccgtca ccaagtcagc aatcgcaaat
    20881 tgaggcagac cagattgacg gaagccaaat acttcaccag gaccacgtaa ttccagatct
    20941 ttttcgctta gtacaaaacc attggtggtc tctgtcatga ttttcatccg ttccacacct
    21001 agttcatttt taggattagc aactaagata cagtaggaag catcacttcc gcgtccaaca
    21061 cgtccacgaa gttgatgcag ctgtgccaat ccaaatcgat ccgcatccat gatcagcatg
    21121 acggtcgcgt tcggcacgtt tacccctact tcaattacag tagtcgaaac taatatctgc
    21181 atctggttgt ctttgaaggc ttccatgatt acttcttttt cctgattctt cattttacca
    21241 tgtaacaagc caacttcata ttttggtgca aatagttcac gaagtttttc atagatttcc
    21301 actgcatttt ttacatctaa tgcttctgat tcttcaatca gcggacagat gacatacatt
    21361 tgatgtcctc ttgccagttc ttttttcgtc caatccagta cactgtccaa ttgaggtgtt
    21421 cttacccagc gtgtttcgat cgggatacgt ccagctggaa gttcatcaat gatagataca
    21481 tccatctcac catatgctgt aatagctaat gttctaggaa tcggagtcgc tgtcataaat
    21541 aagacatctg gtcgccagcc tttttcacga agtactttcc gctggttcac gccaaaacgg
    21601 tgctgctcat ccgtaataac tagacctaaa ttctgaaatt cgactccatc ttggatcaat
    21661 gcatgtgtgc cgatcacgat gttaatttcc ccttgctcta gtttttcaag gagctcacga
    21721 cgttcttttg ctttcgttga ccccgtaagc aatgcgatgg ttacttcatt gggatcaaac
    21781 agctgttgca agctttccat atgttgttgt gccaagattt ctgtaggcac catcaacgca
    21841 ccttggaaac ctgctgtaac ggtagcgtat aacgcaatcg cggcaaccac tgtttttccg
    21901 ctacctacat ctccctgcaa aagtcgctgc atatgctgcg gactgcgaag atcccgacag
    21961 atttcattcg ttacacgttt ttgtgcattt gtcagttcaa aaggtagctt ttgagtaaat
    22021 tctttcaatt tttggacatc ataaaggatc tccaaaccat tcgtttctgc tttttctgct
    22081 tttttcaatc cttgaagacg catttgaaat aaaaagaact cttcaaaaac gacacgtcgt
    22141 ttggcttgat ggttttcttc gtggtctttt ggaaaatgca tacttcttac agcagaagct
    22201 cggtcaagca gtcgatattt ctccacaagg ttgtttggca gattttcttc aatcaatgtg
    22261 ccgtattctt gaaacccttt tttgattaga tcaactaatg tcgtttgacg aattgatttg
    22321 ttgacgtgat atatcggcga aaagtcatcc attgtttgag aacctaaaat cttcatcccg
    22381 ttcaatgctt tacgctttgc atcccattta ccgtatacgg cgatttcctc cgataaaatg
    22441 actttttctt ttagataagg ttgattaaaa aaagaaacgt tgaacacatc atgttcttgc
    22501 atcatacgaa attgcaatcg gcttttttta taaccaaatc gattcatcac aggaggcgag
    22561 actacgattc ctttcaaagt aaccttttct tgatcttgga tctcgtttag atttctttcc
    22621 tgaatatctt cgtagcggaa aggataataa gtcagtaagt cttcaatcgt ttggatgccc
    22681 aagctggcaa gactagctgc tcttttttct ccgactcctg ccagcatagt gattggatca
    22741 tctaaagaac gcataaattt ttcctccctc ccttaacaaa aaaaggactt ggtcagcgat
    22801 catacctgat cactaaccga aggcctttta tgaatgaata ctgtgttaga aagatattct
    22861 gacacagaaa ttattcagca gaaaatagat aaggatagac aggctgatct ccttggtgga
    22921 tctcgatttc caaatcagga tgtttttctg ttagtttctc agcaaattca tttgcttgtt
    22981 caactgttcc ttcatcacct gccaagatcg ttacgatctc tgtgtcctca ttgatcattt
    23041 gttctagtgt acttaacgct gttgctgtga tatctgggtt agagatcagg atttttccat
    23101 cgatcatgcc caaaaagtct ccttctttga tctctacacc gtcaattgag gtatctctga
    23161 tcgcgtgagt gacttgaccg ctcacaacgc tagacaacat ttctgtcatc gtttctttgt
    23221 tttcttctaa agaagcttga tcattgaatg aaagcatagc tgtcaatcct tgggaaatcg
    23281 ttcttgaagg aacaactgct actgggatat ctgctacttc tgctgcttga tcagctgcca
    23341 tgaagatgtt cttattgttt ggcaagacaa tcactttatc tgcgtttact tcttgaatcg
    23401 ctttaacaat gtcttccgta cttggattca ttgtttgccc accactgata acataattag
    23461 cgcctaaact ttcaaacagc tcttggacgc ctttaccagc agcgatcgct acgataccat
    23521 acgggatacg tggtgtttct tttttcgcaa actcgttcac ttcttcatcg tgttccaaaa
    23581 tcgtctcatg ctgcaagcgc atgttgtcga ctttgatttt gactaatgaa ccgaattttt
    23641 gtccataatt caagacttct cctgggtttt ctgtatgcac gtggactttg atgatctcgt
    23701 catcattgac cacaagtaaa gaatctccta taccatctaa atagttacgg aattcttcgt
    23761 aatcgaatgt actatcgact gtaggtcctt ctccgatttt taccatgatt tctgtacagt
    23821 aaccaaaatg gatatcttct gtcgccattt gaccttggac actgcgatga tgttctgcat
    23881 tgaccatatc atccatttct gctggagaag gttcatatgt ttcatccgct tggaattctc
    23941 cattcaatgc acttaaaaag ccctcataaa caaacagcag tccttgccct ccactgtcta
    24001 cgacaccgac ttcttttagg actggaagca agtctggagt tttagctaaa gcacgctttg
    24061 ccccttttac aacagctgtc attacttcaa cgacatcatc agtacttttt gctttctttt
    24121 ctccagcttt cgctgcttct ctagctactg tcaaaatcgt tccttctact ggtttcatta
    24181 ctgctttata cgcagtttca actccatgag tgaaggctgc agcaagatcg gaagcattta
    24241 atgagacaac atctggaatt tgcttagaaa atccacgaaa caattgagac aaaataacgc
    24301 cggaatttcc gcgcgcaccc atcagcaatc cttttgaaag cattgctgct aattctccta
    24361 ctttttctga ggttgaatct actactgcct tagctccact agtcatagat agattcatat
    24421 ttgttcccgt atcgccatct ggcacaggga atacatttaa tgaattgacg tattctgcat
    24481 ttacttgcag acgattcgca cctgcctgga ccatttcctg gaactggctt gcacttattt
    24541 ctgttacact cacctagata atcctccttc agatcgactg ctgagttacc agccagtctc
    24601 ctgcctagtc tggcagtaca cgaacacctt gtacaaagac attaactgaa ttggcagtta
    24661 cgcctaataa tgcctctaga ttgtatttca ctttttcttg gacgttgcgg gaaacttcag
    24721 agattttcgt accatagctg ataatcgtat aaacatctac agcaatacca ttatcttctt
    24781 ggcggacaac tactcctctt gagtaatttt ctttacgcaa aatctcagta atattatctt
    24841 taatttgatt tttgctggcc attcctacaa caccgaacac atctgtcgcg gcgccaccaa
    24901 caactgttgc aattacatca ttggtaattt caatggttcc agctgacgtt tgaattttta
    24961 cagccatgaa tgagcctcct taacgggtaa aaaactccca tttttcttct atcatatttt
    25021 atcatagcgc aacttgaaat aaaaggcagt tacccaaagt actcaaaaac cttgcaaaat
    25081 ttcaggtata accttgttta atttacaatt attcttctca attgtcaatt gtatctatga
    25141 aaatgtactt gcaaatttcc attctatatg atatgttagt ctggtatgag ctgaattatt
    25201 ctattgctcc aaatcaaggc aaaggaggaa attaccatgg caaaagtttg ttactttaca
    25261 ggtcgcaaga caaaaagtgg aaacaaccgt tcacacgcta tgaactcaac aaaacgtact
    25321 gttaaaccta acttacaaaa agttcgcgtt atggtcgacg gaaaacctaa aaaagtttgg
    25381 gtgtcaactc gtgctttgaa atcaggaaaa gttgaacgcg tttaattgaa tgatacttgg
    25441 accgttcaga tagtgaacgg tttt
//
LOCUS       DBNICI010000024        25349 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.024, whole genome shotgun sequence.
ACCESSION   DBNICI010000024 DBNICI010000000
VERSION     DBNICI010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 25349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.024"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="AC1ZCC_000788"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="AC1ZCC_000788"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HIQ1629060.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="AC1ZCC_000789"
     CDS             complement(937..1518)
                     /locus_tag="AC1ZCC_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIQ1629061.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="AC1ZCC_000790"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="AC1ZCC_000790"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HIQ1629062.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="AC1ZCC_000791"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="AC1ZCC_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIQ1629063.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="AC1ZCC_000792"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="AC1ZCC_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIQ1629064.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="AC1ZCC_000793"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="AC1ZCC_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIQ1629065.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="AC1ZCC_000794"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="AC1ZCC_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HIQ1629066.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="AC1ZCC_000795"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="AC1ZCC_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HIQ1629067.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="AC1ZCC_000796"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="AC1ZCC_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIQ1629068.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="AC1ZCC_000797"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="AC1ZCC_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIQ1629069.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="AC1ZCC_000798"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="AC1ZCC_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIQ1629070.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="AC1ZCC_000799"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="AC1ZCC_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIQ1629071.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="AC1ZCC_000800"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="AC1ZCC_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HIQ1629072.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="AC1ZCC_000801"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="AC1ZCC_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIQ1629073.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="AC1ZCC_000802"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="AC1ZCC_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIQ1629074.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="AC1ZCC_000803"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="AC1ZCC_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIQ1629075.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="AC1ZCC_000804"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="AC1ZCC_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIQ1629076.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="AC1ZCC_000805"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="AC1ZCC_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIQ1629077.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="AC1ZCC_000806"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="AC1ZCC_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIQ1629078.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="AC1ZCC_000807"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="AC1ZCC_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIQ1629079.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="AC1ZCC_000808"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="AC1ZCC_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIQ1629080.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="AC1ZCC_000809"
     CDS             13972..14157
                     /locus_tag="AC1ZCC_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HIQ1629081.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="AC1ZCC_000810"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="AC1ZCC_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIQ1629082.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="AC1ZCC_000811"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="AC1ZCC_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIQ1629083.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="AC1ZCC_000812"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="AC1ZCC_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIQ1629084.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="AC1ZCC_000813"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="AC1ZCC_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIQ1629085.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="AC1ZCC_000814"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="AC1ZCC_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIQ1629086.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="AC1ZCC_000815"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="AC1ZCC_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIQ1629087.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="AC1ZCC_000816"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="AC1ZCC_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIQ1629088.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="AC1ZCC_000817"
     CDS             18232..18879
                     /locus_tag="AC1ZCC_000817"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIQ1629089.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="AC1ZCC_000818"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="AC1ZCC_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIQ1629090.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="AC1ZCC_000819"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="AC1ZCC_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIQ1629091.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="AC1ZCC_000820"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="AC1ZCC_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIQ1629092.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="AC1ZCC_000821"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="AC1ZCC_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIQ1629093.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="AC1ZCC_000822"
     CDS             20317..21255
                     /locus_tag="AC1ZCC_000822"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIQ1629094.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="AC1ZCC_000823"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="AC1ZCC_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIQ1629095.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="AC1ZCC_000824"
     CDS             21935..22774
                     /locus_tag="AC1ZCC_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HIQ1629096.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="AC1ZCC_000825"
     CDS             22750..23619
                     /locus_tag="AC1ZCC_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HIQ1629097.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="AC1ZCC_000826"
     CDS             23609..24406
                     /locus_tag="AC1ZCC_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HIQ1629098.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..>25349
                     /gene="truA"
                     /locus_tag="AC1ZCC_000827"
     CDS             24609..>25349
                     /gene="truA"
                     /locus_tag="AC1ZCC_000827"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIQ1629099.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKY"
BASE COUNT         8228 a         4681 c         5458 g         6982 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caagaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggta gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttgaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcgagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttataa aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcatacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgattgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac cctcttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaag tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacatcctg
    25321 aaggacttta tttgtatgaa gtgaaatat
//
LOCUS       DBNICI010000025        25155 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.025, whole genome shotgun sequence.
ACCESSION   DBNICI010000025 DBNICI010000000
VERSION     DBNICI010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 25155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.025"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(119..343)
                     /locus_tag="AC1ZCC_000828"
     CDS             complement(119..343)
                     /locus_tag="AC1ZCC_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HIQ1629100.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(541..2121)
                     /locus_tag="AC1ZCC_000829"
     CDS             complement(541..2121)
                     /locus_tag="AC1ZCC_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HIQ1629101.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2522..3898)
                     /locus_tag="AC1ZCC_000830"
     CDS             complement(2522..3898)
                     /locus_tag="AC1ZCC_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIQ1629102.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4026..5144)
                     /locus_tag="AC1ZCC_000831"
     CDS             complement(4026..5144)
                     /locus_tag="AC1ZCC_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIQ1629103.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5286..5720
                     /locus_tag="AC1ZCC_000832"
     CDS             5286..5720
                     /locus_tag="AC1ZCC_000832"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1629104.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5798..6331)
                     /gene="ntdP"
                     /locus_tag="AC1ZCC_000833"
     CDS             complement(5798..6331)
                     /gene="ntdP"
                     /locus_tag="AC1ZCC_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017110 - nucleoside diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside tri-diphosphate phosphatase"
                     /protein_id="HIQ1629105.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6425..7603)
                     /gene="mutY"
                     /locus_tag="AC1ZCC_000834"
     CDS             complement(6425..7603)
                     /gene="mutY"
                     /locus_tag="AC1ZCC_000834"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HIQ1629106.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7596..8393)
                     /gene="recX"
                     /locus_tag="AC1ZCC_000835"
     CDS             complement(7596..8393)
                     /gene="recX"
                     /locus_tag="AC1ZCC_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HIQ1629107.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8500..9882
                     /gene="rlmD"
                     /locus_tag="AC1ZCC_000836"
     CDS             8500..9882
                     /gene="rlmD"
                     /locus_tag="AC1ZCC_000836"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIQ1629108.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10056..10634)
                     /locus_tag="AC1ZCC_000837"
     CDS             complement(10056..10634)
                     /locus_tag="AC1ZCC_000837"
                     /EC_number="1.14.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase auxiliary
                     activity family 9 protein"
                     /protein_id="HIQ1629109.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10956..11426
                     /locus_tag="AC1ZCC_000838"
     CDS             10956..11426
                     /locus_tag="AC1ZCC_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HIQ1629110.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11534..12760)
                     /locus_tag="AC1ZCC_000839"
     CDS             complement(11534..12760)
                     /locus_tag="AC1ZCC_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIQ1629111.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13514..13714)
                     /locus_tag="AC1ZCC_000840"
     CDS             complement(13514..13714)
                     /locus_tag="AC1ZCC_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIQ1629112.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13848..14456)
                     /locus_tag="AC1ZCC_000841"
     CDS             complement(13848..14456)
                     /locus_tag="AC1ZCC_000841"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HIQ1629113.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14541..15011)
                     /locus_tag="AC1ZCC_000842"
     CDS             complement(14541..15011)
                     /locus_tag="AC1ZCC_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HIQ1629114.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(15059..15973)
                     /locus_tag="AC1ZCC_000843"
     CDS             complement(15059..15973)
                     /locus_tag="AC1ZCC_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIQ1629115.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            16119..16922
                     /locus_tag="AC1ZCC_000844"
     CDS             16119..16922
                     /locus_tag="AC1ZCC_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HIQ1629116.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            17100..18047
                     /locus_tag="AC1ZCC_000845"
     CDS             17100..18047
                     /locus_tag="AC1ZCC_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HIQ1629117.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(18115..18408)
                     /locus_tag="AC1ZCC_000846"
     CDS             complement(18115..18408)
                     /locus_tag="AC1ZCC_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HIQ1629118.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(18436..19101)
                     /locus_tag="AC1ZCC_000847"
     CDS             complement(18436..19101)
                     /locus_tag="AC1ZCC_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIQ1629119.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(19241..19873)
                     /locus_tag="AC1ZCC_000848"
     CDS             complement(19241..19873)
                     /locus_tag="AC1ZCC_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIQ1629120.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(19880..20947)
                     /gene="liaS"
                     /locus_tag="AC1ZCC_000849"
     CDS             complement(19880..20947)
                     /gene="liaS"
                     /locus_tag="AC1ZCC_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HIQ1629121.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLIAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(20944..21675)
                     /gene="liaF"
                     /locus_tag="AC1ZCC_000850"
     CDS             complement(20944..21675)
                     /gene="liaF"
                     /locus_tag="AC1ZCC_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HIQ1629122.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(21844..22323)
                     /gene="greA"
                     /locus_tag="AC1ZCC_000851"
     CDS             complement(21844..22323)
                     /gene="greA"
                     /locus_tag="AC1ZCC_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIQ1629123.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(22459..23634)
                     /gene="mltG"
                     /locus_tag="AC1ZCC_000852"
     CDS             complement(22459..23634)
                     /gene="mltG"
                     /locus_tag="AC1ZCC_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIQ1629124.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            23838..25007
                     /locus_tag="AC1ZCC_000853"
     CDS             23838..25007
                     /locus_tag="AC1ZCC_000853"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIQ1629125.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSR
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT         7557 a         5072 c         4052 g         8474 t
ORIGIN      
        1 aagcagaagc aactccttcg gaaataagct gaaattcacg aaaattgctt ttcaaatttt
       61 tgtgaattcc agcttatttc ttggagttaa acgcttctgt ctcagcctct ttttctttct
      121 agctttttga tttttctctt ttcttttgta cttttctctt ttcattttca gttctacgcg
      181 tgtctttatt tttaactttt tcctctttat ggatgattga tcgtactttt tgtgcaattt
      241 gctcgttttt gtcccagtct tttacatcta ataatcgaca taatcgtaac atctcttctt
      301 ttttctgttt ttttctttca cgtctttgcc attctgtttc catgtgtcac actctccttt
      361 ctttaatcat atgtacgcaa aataattttt atattttttt gattagacat ttctatgatt
      421 ttaatatagt aaaaagagcc gtcacggaaa atccgtcacg gctctaaaca aaatatgaga
      481 ggctgttttt gtttggctat tcttattact tgatgcaggg cagcttgtgt caagacttct
      541 ttacatcaaa ctttttaatt ttacattggg atatttatcc gcaaaccagc gttctgcaaa
      601 ttggttctcg aataagaaca acggttggtc gaaacggtca cgagccaaga tattgcgact
      661 tgaactcatc ttctcatcca aatcttctgg atcgatccag cgagcgattt tgtgtcccat
      721 tggagacatg attacttcgg cgttatactc atttaacata cgatattgga atacttcgaa
      781 ttgtagctga ccaacagctc caatgatata ttcttccgtt agatatgttt tataaagctg
      841 aatcgctcct tcttgaacta attgatagat tcctttgtgg aaggattttt gtttcatgac
      901 attttttgca gtcactttca taaacagctc tggagtaaat gaagggagtt cttcataagc
      961 aacttttagt tttccttcga acaatgtatc cccaatctga taattgcctg tatcatatac
     1021 accgatgata tcgcctgcta ctgctttttc gacgttttct ctcgaatctg ccataaattg
     1081 tgtgacattg cttaatttca gttttttatt tgtacgttcc aaccagacat ccattcctcg
     1141 ttcgaatgtt ccagaacaga ttcggacaaa agcgattcgg tcacgatggg ctggattcat
     1201 atttgcttgg atcttgaata cgaaacctga aaattcttcc tcataaggac ttaccatctg
     1261 atcatcttgt gttttgtgtc cataaggagc tggtgccaat tcaacgaaag tctctaaaaa
     1321 agtctgtaca ccgaagttag tcaatgcaga accaaaaaat actggtgtct gatcgccacg
     1381 agcaattttt tcttgattaa atgcatctcc agcctctttg attagttcta cttcttctaa
     1441 gacttgttca tataaggaat tttcagttag aggatgattt tcgggaagca gtccatcttc
     1501 atccaattct actaatcgtt catcctgata attttccgga cgataaaact ctacacgatt
     1561 atggtagata tcataaagac cttctagccc tttacccata ccgattggcc aattcatcgg
     1621 ataagattcg atatctaata gctcttctaa ttcttctaac aattccaatg gttcacgacc
     1681 atcacgatct agtttgttga taaaagtaaa tatcgggatg ccacgttttt ttacaacttg
     1741 gaataatttc tttgtctgtg cttcgatccc ttttgcacta tcgattacca tgaccgcact
     1801 atccacggcc atcaaagtac gataagtatc ttcagaaaaa tcttcatgtc ctggtgtatc
     1861 caagatattt actcgtttcc cttgataatc aaactgcata acagaactag taacagaaat
     1921 cccacgttgt ttctcgattt ccatccagtc tgatttggca aaattacctg tttttttgcc
     1981 ttttacggtc ccggcttgac ggatagcccc accaaacaat agtaattgtt ctgtaatcgt
     2041 tgttttccca gcatccggat gggaaatgat cgcaaaagtt ctgcgattgt ccacttgttg
     2101 ctttaatttt ggattattca taacctaact ccatttcttc cattcaatta tttatatttt
     2161 ctctaggcac agattgtccg atctcttaaa gaaagacaat ttcatatagg gctgtgacaa
     2221 aagccgtgta gcagtctctt tttccgagta aacggcgttc tagagacagc ctatcagcaa
     2281 taagcccacc caagccaaaa atacgaaaag ctgtttttgc ttggctgctc ttattactta
     2341 atgcaggata gctttttcac aacctttgtc aactactaca actgaatcag tatagcatgg
     2401 attcagcgaa tgaaaaagcc accaaaccat aagagtcaaa tatttttttg tcagctgact
     2461 aaaagaaaag acgtgttgct tgtttattca agcaacacgt ctttcataac caagtttcca
     2521 attatcttct tggctcgtca tcttcgaatt cttttctgaa gtatcttcgt tcttcttcag
     2581 gttccgcttc tgtttcttcc ggatcatgaa aaatgacacg gattttaagg attcgagaac
     2641 cttccatttc ttcagaaacc agcgtaatat tatcgacatc aaacgataac ttttcgcctt
     2701 catccggaat catgcctaat gctgtaatca aataaccagc catcgtatct acatcactca
     2761 tatgcagatc actgtcaaaa gcttcattga actcatcaat cagcatacgt ccttggatga
     2821 tatattcgtg ttcatcgatt ttttcataaa gattctcaac ttcatctgat tcatcatcga
     2881 tttctccgac gatttcttct aaaaggtctt ctagtgttgc taacccaacg acgccgccat
     2941 attcatctaa taaaatagcc atctgattct gcgtcttttt catttcatat aacagatcat
     3001 caataaagac cgtttcagga acgaataaag gctcctgcat gattttcttg atatctaaat
     3061 tatcaaagcc gaatttatgg gcagctttta gtaaattctt tgtatgaagg attccgacta
     3121 ctttatcttt gtcttcgtta taaacaggga ttcttgaata gttttcggat aaaacttcat
     3181 tcacattttc ttccacagtg tcgttgatat caatcataaa tgcatccgtt cgtgggacca
     3241 tgacttctcg ggcaactttc gtatcaagag aaaaaacccc ttggagcatt tctaattctt
     3301 cattattcaa tacgccttcg ttttcaagca tataacgcat ttcatctctg gtcatttttg
     3361 aatcttcatc atcaaaagtc atcggcgtga tacgcgccaa taaacttgtg gaagcagaaa
     3421 gcagccagac gaagggctta gctactacac caatcaagcg tatcagccct gaagtgaact
     3481 ttgcgacttc ttctgattta tttaaagcaa tacgtttagg gtacaattct ccaaaaacaa
     3541 tcgatacata ggtcaaaata gccagcacaa tgatatttgc aatatttttt gccgctgtgc
     3601 cgccacctaa taccggagct aaccgttccg ataaagtatt agctagtgaa gcaccagaca
     3661 aaatgttgac caaagtgatt cctacttgga ttgtcgatag gaaattattt ggattctgca
     3721 atatttttaa aagcttttga gatttcacat ctccttcatc agctttttgc tcgattcgat
     3781 tcttattgat cgaaacaaca gcaatttcgg aagctgctaa aaaagcattg atcaatgtca
     3841 aaacgatcaa taataaaatc tgcgctaaca gcgactgact ctcagggtca gcattcataa
     3901 tcgcattctc tccttataaa attaattttt agtttacgat agaaaaaaac gtggcactat
     3961 agccacgtta aaatggtatc acaaagcaat tttctttgca ataaaaacaa gtgtactaaa
     4021 actacctacg aggtcgtagc aattttcttc ttgttttcat tttgcttgtc ttctttgacg
     4081 attttgatta tataggaaat gatcgtcttg cagattgcgt aaaaaggaac acctaagaag
     4141 ataccaagca atccggcaat atttccagct actagaagaa cgataatgat cgttaatgga
     4201 tggattttca gtgatttccc aattacatta ggataaatga tattcccatc caactgttgg
     4261 acgattagca caaccacaca acaaagtaat gcttttaccg gatcattgaa gactgttact
     4321 agaaatgcag gtgctagtcc tagataaggt cctaaatatg gaattaggtt tgtcaatccc
     4381 gcaatgaccc caaataagaa agcataatcc acaccaatta ccatatatcc taaaaatgtg
     4441 aaggtaccaa cgaataaaca ttcgattgct tgtccgctaa tgtaattagc caatgtttta
     4501 ttcagttgcc ccaataactc cacaatttgg tttcttcttt tttctgggaa aaactgttgg
     4561 atgtttggca ctagtttttc tccgtctttc aacatataga acaagatgaa tggtgctgta
     4621 atgattacta cggttgccga agcgatcgtt ccaacgatcg aacctaaact attggatact
     4681 ccactaagga attgctgaat gattgtcccg taagaaatgt ctaattgctc aaaatattta
     4741 ttgatatcta cttccttgaa caaaggcaat tcggctacct cttttaacca gctttctacc
     4801 tgtttgatga aatcaggcat attagatgcc aatgaggaaa tttgctgtac tagacttggg
     4861 atgacactaa gtaaaatcca aataattgct gcaactaaaa ggagcagcac aattgcgaca
     4921 gcccatattc ttttgatttt tgctttcatc aacagcttga caataggatt caataaatag
     4981 taaagaaagc ctgcaatcag tacaggtgca aataacgtgg aaaaaaatgt cccgatcggt
     5041 tcgaaaataa agtttatttc tgatgaaatc atgatcaatg cagctatgat caataattca
     5101 agcgaccaaa acattagttt agactgtcta agtttttcaa acaatagatt tcctcctttc
     5161 tttcttacta gtatatatta tattcgttaa aaattctttt attctttttt tatttttgaa
     5221 aacttgccat actttttatt tgttttattt tccagtatac tatacgaaag gaaggtggct
     5281 agaccatgaa agttgtacaa acaaaagata caatgagcaa tatttactta gatgctgtac
     5341 ggatcaggca tcaagttttt gtagtcgaac agggtgtgcc tttgtctcga gaaatagaca
     5401 aagatgaagc ccattgtatc cacttcgtcc tgtactcaga taaaaaagaa ccgcaaggaa
     5461 ctgtacgtct tttgccttta gaaaacggaa aaatgaaatt acagcggatg gcgattcttt
     5521 ctgaatacag acaccaaggg ttaggaaaaa tattgattga agaagcagaa aactttgcta
     5581 agaatcaagg ctataatacg attttattag gggcacagtc cacagctgaa actttttacg
     5641 aaaaactagg atatacagct tacggcgatc cttttgaaga tgctggcatg cctcatattt
     5701 atatgaaaaa gactttataa aaatttattt cgaaaaaagc aaaaaacagc aagcttttga
     5761 gaaaaatctc tcactggctt actgttcttt tttgtgttta cttacgcgac aattgcttgt
     5821 aacggttata ccaaatatca atatagcctt ccgagaacgg gccatctcca ttattgatcc
     5881 agtcaaccag aatcttcaca ttttctttta ggatgaagtc aatatcgttc gggtaattca
     5941 tcattttgct gtgcatctca tattcatcga catccaatag tcgtttctcg ccgtcaggaa
     6001 ataccttgat atccaagtca tagtcgatat atttcaaggc ctcatcgtcc agcaaaaaag
     6061 gtgaagctag attacaataa taggaaaccc ccttttctct gatcattgct attacattga
     6121 accagtattt tttatgaaaa taaacaatcg ctggttcgcg cgttacccat cggcgtccat
     6181 cagattcagt gactaatgtg tgatcattca cgccgatcat agagtgttcg cttgttttta
     6241 ataccatggt atctcgccac gttcgatgca atttgccgtc gtgcttgtaa ctttggatcg
     6301 tcacaaactc tccttctttt ggaactccca tagcaaccct tccttaagca gcattttgtg
     6361 ctttgtgttt gggtgttcat tacaccgaca tcataattat tatatcaata aaaatgagct
     6421 ttgcctagaa aggattccta ttgtttcgat ttttctctaa ctgatctacc agtttcattt
     6481 ggaccttagg aaaaacgacc gaatcaaatg catcaggctt caaccacttc gaagttttgt
     6541 tctcggtaaa ttcttgttct gctccctcgg ttgcccgtcc gtaaaaaagg agaacatgcc
     6601 atttcagatg actaaaaata tgagtaactt ctcctaaatg tcgtgtctgc caaacaaaga
     6661 gttcctgctt ttcaaatggg agattttgct cttcttctgc tactaggtca tcaaacagat
     6721 cgatttctgg ctttgtttcc cattcttttt tcaatcgctc atactcctct tgtgtcactt
     6781 ccatcattgg aaatgtccac atattcgcca gcaatttttg gctatctctt tcttcaaaat
     6841 aatacgctcc agaatgattt tgaagagctg cactgatata ataaacgtct tttggctttg
     6901 ctttttttgt ttttacagga aaactcgttt ggattcctcg tttgttagct aaacaaaagg
     6961 cttggatcgg acaagtctca cactttggag aagttggtgt acagatagct gaacctagat
     7021 ccatcattgc ttggttgaat tcacctggat gcttttcatc aatgattttc cgcatcgctt
     7081 catcaaagat tttcctgcta gaagcttttg cgatgtctgc ttcaatgcaa aacaatctgc
     7141 tgactactcg catcacgttg ccgtcaactg caggttctgg aagtccaaat gcgatacttg
     7201 caatcgcgcc tgtcgtatat ggtcctattc ccttcaatga gctaatttct tcaggcgttt
     7261 gcggcatttc cccatcaaat tctgacatga tctgtttagc agcagcttgg atgttccggg
     7321 ctcttgagta ataaccaagg ccctcccatg cttttaaaag cttttcttct ggagcatttg
     7381 ctagctcttc aattgtagga aaccattcca tgaaacgata aaaataatcg atcactgtgt
     7441 ctactctggt ttgctgaagc atgatctcag agatccagat acgataagga tcacgattat
     7501 agcgccaagg caggtttctt ttttcttgtt cataccattg aatgaactga tcttgaaatt
     7561 cttttgtttc tttatctgtc catttttccc attctttatt cactttcctc ttccttttcc
     7621 gtaatgaatc gctggatcat atccaaatca aatccttttt gatataagct ttgctttact
     7681 tttagatttc tttttttagg ttcaaacctt aaatttcttc gccatagttt atctccttgt
     7741 ttgactaaat aatcatattc tatttcttga tctgcttctt gcggaagatc agctatcgct
     7801 tgttggatca cttcttgaga aaagcctttc gtcatcaatg tttgttggat ttttctcaat
     7861 aattcttttt ggctttttcc gtgatttttt ccaaacgctt tttcggctgt ctgatgtgca
     7921 ttttgaactt ggtcttcaaa tgtatattgg ctgagcgctt gttcaatgat ttcaggtttc
     7981 aaaccttttt gctgcatctg ctgcgctaat ttctttggtc ctttgtcact taagcgcata
     8041 ttcgtgcgca cataactttc cccatagacc aaatcatcga caaggtccaa ttcttttaag
     8101 cgggctacga ttttatttcg atcttcttga aggatttcct tttcttttaa gtatgttcgg
     8161 atttcttttt ccgatcgcaa ctgataactt aagtaattca ttgctaggtg aaaaccgaaa
     8221 tcagctccgc tactcttctt gatctcttgg atagtttctt cgtctaattc ccgtcctttt
     8281 aataatcgat agcgtactaa tacatcttca gatacttgta aagcttctcc ttcagataaa
     8341 tctacgcgat agaaagggcc tttgccttta ctgattctcg taacggttaa cataaacatc
     8401 ctcctcgaac atatattctc tatcatacca cagaaaatcc ccgtaatcag gtattatatg
     8461 ttagaataaa ccacagaaaa aagaaaaagg gagatactta tggcagaaac aattgtcaaa
     8521 actggacaaa cggttccttt gaaaatcaag cgtttaggca taaatggtga aggaatcggt
     8581 tatttcaaac ggttgatcat atttgttcca tatgcactac caaaagaaga agtactagtg
     8641 aaaatcacga aagcaactcc tcgctatgca gaaggagaat taataaaagt caaaaaaaca
     8701 agcaaagatc gcgtagtggc accatgtcct gtttactatg aatgcggtgg ctgtcaatta
     8761 caacacttag cttatcaagc tcaattggat tttaaaaagg atctgctttt gcaagcattg
     8821 gaaaaattca aaccagcagg ctttcgatct tatcaacttt tgccgacgat cgggatggat
     8881 gagccttggc attatagaaa caaagcacaa tttcagttac gaaaaaataa gcagactcaa
     8941 aaaattgaag ctggtttata cgaagcaaac tctcatcagt tagtccctat aacagactgc
     9001 ttggttcaag aacctcgaac acaaaaagta atgaatactg ttgttcaact attgaataaa
     9061 tttgatgcac ccatctatga tgaacggaca aacagtggaa tcttccgcac aatcatggtt
     9121 agaatcggtg ttaaaactgg ggagttacaa gttgttttta ttactcgcag taaaaaattc
     9181 ccgcaaaaaa acgcaatgat ccgtgagatc aatgaacgac tgcccgaagt tgtttcaatc
     9241 atgcaaaatg tccaggcaca aaaaacgtct ctcgtgatgg gggatgaaac gatccactta
     9301 tggggaaaag aagccattga agaacaaatc aatgaagtga ctttcgatct ttctcctcgt
     9361 gccttctttc aactcaatcc tcagcagacc gaattgcttt atggcgaagg aatcaaagca
     9421 ttagatgtcc agcctaatga aacgatcgtg gatgcttatt gcggagttgg gacgatcggc
     9481 ttaagtgtag caaaacaagc caaagaagta cggggaatgg ataccgttcc tcaagcaatc
     9541 gctgatgcaa aaaaaaatgc ggaaagatta ggatatcaca acactcgata tgaagtcggg
     9601 acagcagagc aagttctgcc taaatggttg aatgaaggtt ttcagcctga tggtatcatc
     9661 gtagatccac cacgtacggg tttggataac gcattgatcc aatcattatt gaaataccct
     9721 ccaaaaaaac ttgtttatat ctcctgtaat gtttctacac ttgccagaga tctggttaat
     9781 ttaagtaaaa agttcaagat tgaatattta cagtccgtcg acatgtttcc tcagactgca
     9841 cgttgtgaag ttgtcgtgaa gatgacgaga ctcgctgact aaatgaaaga ggttgtgaaa
     9901 aagtctgccc tgtatcaagt aataagaaca gccaaacaaa aatagctctt catgttttat
     9961 gaatactgta cttgtgaccg aagagttaac aggaggacac tatttattta aaaaaagggt
    10021 tgtgacatag tttttgtcac agcccttttt gattattatt ggatattcac gtctatcact
    10081 tgataaaatg catttgtcgt atcgctgata ttccatactg ctaaaatcac attatatcct
    10141 tttcgatcct gcggaagtgt gatcgtttgg ttgactgttt ttggtggaac agttgctttg
    10201 tcgtctattt ttgtgatcaa ttcaaaatca ctaaatttca aaggttgatt aggattccaa
    10261 gttggtttgg taatgtagta atcccaagta cttgtccgat gttgtgctgt taaattccaa
    10321 gtaatattta atgttcctgg attaaccatg tttttatgcc atctgttaga tgtctgttca
    10381 tctaaaggct caaagccgct gactcctgca ctagcaattt ttccatcaat gaatgtattt
    10441 ttggtagctt cgatactttg cggttcccat tgtgctcttc cgacattttg atttaagttt
    10501 cctgctgctg atgttcctag gtatgcacgg cttcctggag aagtaatata accatgcgca
    10561 aaacctggaa tgctaaaacc gaacaaccct aaactaacta acccaataaa caataacgta
    10621 ctaatctttt tcatgtagaa acctccttca tttgataacc acattgtaaa cgctttattg
    10681 tattaacaca aacattatgc ttttataatt ttatgttgaa gtaaaaaagg tgaaaaaagt
    10741 aagatgcaga ttataacaga agtagacgag aaaagtacaa atagaaaaca tgagaatcaa
    10801 atcatatgat ctttttcaaa aacatcattc atataaacaa atgttcaact ttttaataac
    10861 aacaattatc aatttttttc ttacttcaca aactaaatac aggtcttgct gtgagccttc
    10921 atggtaaact aatattatag aaaggagtta gtaaaatgaa gatgataaaa gatattcgtt
    10981 tttgggtttg tgttatagga attgtcatat taggcttttt atcagggttg cttagcggaa
    11041 atcccggaaa atattactat tctttgcaac ttccaccatt tgcaccacct agctggatat
    11101 ttgggccgat gtggacctta ctgtatatat tgatgggtat ttctctttat ttgttactta
    11161 atcacaacaa caaaaaacag cgaaacaatc ttgtcggtct ttttgtgatt cagttcattt
    11221 ttaattttat atggtctgct ctatttttta atttaagaaa tattttcatt gcagcaatcg
    11281 atattacact tttagttatt ttcttgtcag tgcttatgta tcagttgtgg ctgcatcatc
    11341 gcctggcaat gtggctcatg attccttatt atttatgggt actattcgca actctattga
    11401 attattcaat ttatttttta aactaagcgc taaaaagaga cgtcctgtaa tttaacacat
    11461 agaaaaagtg ccacctaagt tagatttttt gtctaattta ggtggcactc tttattaacc
    11521 aaactatttt tttctagtac ccacttgttg ttccatactc attattctgg tcataaccat
    11581 aaccattttg ttcctgtcca gcgtaaggat cataactttg agtatcttga cttggtgtat
    11641 agtactgact tgtatcttct gtttgctgat aataggtatt cggatcttgg tactgatcat
    11701 atgtgttttc tgaataggaa gaataatcgg tgtacccttc actattcgat ccatcaccgt
    11761 ttgtcgaatc gccatcttcc gaatcatcaa agaacatcat tgaagcattc tcatttttca
    11821 aattaggtaa tgtatcgctt gtttttatat ttaattgttt cttcagttca ttttggatcg
    11881 aaagaagatc attttttcct aaaatttgat agtagatatc attgatcatt tgtccttcgc
    11941 cttgcaactg gtcttgtttg attgtttcaa acgccggggt gtagttcgtc gctacatcaa
    12001 gcatgtcatc ccaatctaag tccgtcgtca cattgttctc aactgctttt aaaatttttc
    12061 tataattgct tacgccatct aaacttaaaa ctttattgac gattttcgtc actacttccc
    12121 gctgacgacg ttgtcgaccg atatcgcctt ctggatcttc gtgtcgcatt cgagaatagg
    12181 ctaaaccttt ttctccattc aatgtttgtt cacctactgg tacatggaca ccatccaaag
    12241 taaagtcaat tttattattc actttgatgc caccgaccgc attgatcaaa tcttccatgc
    12301 catccaaatt gatcgttaca taatgatcaa taggaatatc gagcagttgc tcaattgaat
    12361 ccatggccat ttcgacacca ccaaatgcgt aggcatgatt taatttatct accgttccat
    12421 aacctacgat attcgtataa atatctcggt ccaaactgat gatcgttgat tttttttgtt
    12481 tcggattaac tgttactacc atcatcgtat cagaacgtcc ttgttccgtt cttcctaatc
    12541 caccggtatc taaacctaat agcaaaaccg aaaaaggttc tttgtcatca atgcttactc
    12601 tctcagaacg tttggaggat attcgattag agtttttaga aattttgttt actgtttggc
    12661 tcgcttctcc cataaatctg attccgtatg ctgataatcc ggcaatcagc aaaacgaata
    12721 cccctaacaa ggttaataga actttttgcc atcgcttcat aatacacctc tgtttcacat
    12781 tttctaaaat attgtctcat ataactgttc aatcagcaaa ttcacttttt aatcataaga
    12841 aaactttaaa aaacattttt gtgtaacgat tattattttt taaaatttct caaaattctt
    12901 aactatcata ccataccgtg aaaaagatcg aaagaccgaa tacacgcagt tcgcaatcgt
    12961 tttatctttt tttatgtatt ttttccagaa aaagcgaaca acaaggtatt ttccaaataa
    13021 tcgtgcagta aaatgatcgc acataccttt cccatctttt taacagtctt tgcctaccgt
    13081 tcagattatc attcgatttc acgacaattt cttcctatga ctggtccttt tgttaaaccc
    13141 atcttctatt cgttttattt ccagctcact gcatggctct taaaatgact cgaactagtg
    13201 attgcattac catcatcttt taatggatag atatgctcct cttcaaagta aaacgatttt
    13261 cttttaaagt aatagaattg acatggtaat gagtatgtat actagtggtt tcacaaatgg
    13321 tcttttattc aacatccaaa aactgaaatt tctttaaaat ccattactaa cgaagaaaat
    13381 gttccttgct tgataattag ttaggacatg tcaagtgaac aaatgcattt acttagccaa
    13441 aaaatctaaa aacttcataa atagacaaaa agacacgact ctctaagaag tcgtgtcttt
    13501 attcaacaaa ttattataat ttagtcactt cagcagcttg aggtccgcgt gcaccttcaa
    13561 caatcgtgaa ttctacttct tgaccttctt ctaggctctt gaagccgtct ccttgaattg
    13621 ctgagaaatg aacaaatacg tcatctccgc cgtctactgt aataaaccca aatccttttt
    13681 cattgttgaa ccatttaacc gttccgtgtt ccataaatgt atatcctcca aaaaagttct
    13741 tgcaagtaac cttgcaagaa cattataccc aatttagaaa acgctagcaa gctttcgctc
    13801 ttttattttc caaattattt ttaagactga tatgacggag gaaatggtta ttttgctgct
    13861 tcgaaacgtt tgtttacttc atcccaatta ataacattcc agaatgctgc aatataatct
    13921 ggtcggacat ttttatattt caaataataa gcatgttccc aaacgtccaa acctagaatt
    13981 ggtgtctttc cttccatcaa tggagaatct tgatttgcag tagaggtaat agctaatttt
    14041 ccattttcca ttacaagcca agcccatcca gaaccaaatc gtccagcggc tgcttttttg
    14101 aattcttctt taaaagaaga aaaatcacca aaagcttcat taatcgcttc ttttatttct
    14161 cctgtaggtt cgccaccagc atttggtgcc atgatttccc agaaaaatga atggttagca
    14221 tgtccgccac cattgttacg tacagctgtc ttgatatctg ttggaatagc gtccatatca
    14281 gataataatt cttctattgt tttttcgcct aattctgggt atttctcaat tgctgaattt
    14341 aaattcgtca cataagtatt gtgatgctta tcatgatgca gatgcatcgt ttcttcgtca
    14401 atataaggtt ccaatgcgtc atatgcataa ggtagatctg gtaatgtgta agtcataaaa
    14461 aaattcctcc tcaatgtttt cttaataatg tctacattac aaagagtaac aaagagaagg
    14521 aaattcttca attgttatgc ctagtcgtct ttttcacttt gacctacttc ttcttcaaaa
    14581 acctcagttt ctttaaaaat aaacaacttg ctgaaaatat agttcgcaag gataacaata
    14641 atttgagtga tgattttcgc cacatactca ttgctgttca taacattgat caacaatacc
    14701 attgccccca tgtccaatcc gtaagaaagg atacggtaga agaagaactt accgcattca
    14761 ataagtgcat gtgtaaaatt cggtgatttt gaatggaata cccaaagttt gttcgttaca
    14821 aaagcaaaca atacagaaca aaaccacgaa atagaattgg ataaggccat acttaaatca
    14881 aaatattggc gtgcgagagc aaaactaaca aaatttacaa tcgtagccaa accaccaaaa
    14941 aagagataga ctagtatctc ccaatatcct tttgcttcta aatatccttt taactgtcga
    15001 tactgcttca tgagccaacc taccttatta aaaattctta tttatcatac tctatatttc
    15061 aataactttc atctttattt aaaaaaatta attttttttg atcctttgcc tttgtctctg
    15121 cttggatcgt cacatgtgta ataccatatt ttaattttaa taaagaaatt atacgaggat
    15181 atatcttttc aatctcgctt agtagctgat cttcaaaatc aatatgtccg tcgaaaataa
    15241 ttatcttttc gctatactgc caaatatgga catgatgaac atcctttacc ccatcgattg
    15301 cttcaatttc ttgttccatt ttttcatagt cgatagcagg tgcactttgt atcaaaatag
    15361 cggcagcttt tttgatcacc attatcgctt ctatcaatat atatatcgaa atgatcagtg
    15421 taataactgg atcgatccag tagatatcga aaaaacggat caaaattgct ccgataatca
    15481 cacttaccga agacagtgta tcacttaaca aatgcaaata actagatttt atattcaaac
    15541 tttcatctgc ttcttgtcgt aataataata ctgagaacaa attcgcgagc aatccaataa
    15601 ctgctactgt tagcatcaaa tgactattta tcttttctgg tgaagtgaac cgcttgaatg
    15661 cttcaacggt taaaaacaag gataatatga tcaatatgaa tgagttgatg aaagcactca
    15721 atatttctaa acgctggtag ccatacgtac gttttttatt tgcttctttt cgggccattt
    15781 tttgtgcccc gtaagaaaaa ataattgcca atgtatcact aaggttatgg aaagcatctg
    15841 aaacaagaga caaactccct gacataatcc ctccaataaa ttctgataaa gtgatcaccg
    15901 cattcaaaac cactactcca aatagttttt ttgatgatct ctgtggctgt gtaggcacta
    15961 ttccttcctc cattttattt atattggata gtctttagac aagaataaca tttttttgat
    16021 atttcaccaa tcattcagct gtttccttaa agcacttgta tttcacttga tacttcgcta
    16081 gaattgcaag tgatgacact actagatgag gagtacttat gacaacaaaa caattaatta
    16141 tcagttcctt tatacgattc aaagacctaa aggaggcgag aaatgtccca ttttggaaag
    16201 ccattgtcta tcttttagtt ttgagtataa ttatggcttt gccaatctct tttcaagttt
    16261 ttcaagtctt aaacaacatt caattggatg ggcaaaagat tgctactcgc attcctgatt
    16321 ttacaatcca aaacgggcag cttcaaaccg aagaaaaaga aggattcatt taccaaacaa
    16381 actccattat cttcacattt gatccagagg gaaaacgaac agaacaggac atctctactg
    16441 atttgatggg gaattttgtt agtgtcggga tgttaaaaaa caaacttatc atagctcttc
    16501 ctaatacagg cacgacttct gctttgttga acaataacca attagaactt ccctacacca
    16561 atgaatcttt aaaaaactta acaggtaagc agctccgttc ttttttaagt gaagcatcta
    16621 taccactttg gataaaagtc ctgacattta tattttctat ttatcctagt tttctgaatc
    16681 tgctgatcac tttgctattt accaacattg ctgcttttct ctatgcacgc tttcgactaa
    16741 caaaagctac ttttcttgat tgtttaaaaa cattgatcta ttcaatttca ttgcctacta
    16801 taattgcaac aatcttgatg gtctttcttc cttcttttga cactagcgcc ttcattgctt
    16861 ttgcaggaat ctttattttt gctcaagctg tcaaaggatg ggcaaaaata tcgatcaatt
    16921 aagaaataaa gacaactcgt tatttctact tcaatagacc cttttagagg ttgtgacatt
    16981 tttttgtcac agcccctttt ttatttgaaa aatatttctt tctcaaaaaa caagcaaggc
    17041 gcttcaaagt tcttcttgta tgcgctacac ttagagtaaa gaaaggcggg aaattaacta
    17101 tgtcagaaag aatcatttat gcagatcgtc catttcgtga tgaatttatc gaagaaatcc
    17161 atactatagc gcctggttat atctttaaaa cagaattaca accagaggat ctacaaaatg
    17221 tagagatcag tttgggctgg aactcagact accaagaaaa actattagcc tcttctacct
    17281 tgaaatgggt ccaatctatc tcagctggtg ttgatcagct accccttgag actttttcaa
    17341 agaaacatat tttactttca aatggtagtg gcatccacag tgaatcgatt acggagcatg
    17401 tcttaggaat catccttggg tattctcgtg gtctttttca agcacaaaag gcacagagtg
    17461 acatgaaatg gttaggttca tcggtccatt accaatctat ccaagggaaa aaaatactaa
    17521 tcattggaac tggccatatt ggaaaaatga ttgcaaaaaa atgcaaagca ttagacatga
    17581 actgctatgg aatcaataca tccggtcatc cagcagaagg aatggaaaag actttttctt
    17641 taaatcaact aaaggaagtt cttcctgaag tagatttcgt agtcaatatc ttacctctaa
    17701 cggaggaaac aactgggctc tttgatcagc atatgtttga acactttgat ccaaaatctg
    17761 tattcatcaa tgttggccgt ggtgcttctg ttaagacaca agatctggtg caggctttaa
    17821 ataaccagca actttccttt gcagcattag acgtttttga ggaagagcct cttccacaag
    17881 atcatcctct ttggaaaatg gacaatgtac tgattacttc ccacattgca ggtttgacac
    17941 ctgattttca aaagaaatta atggctattt ttttaaccaa tctgaaaagc tactttgcca
    18001 cccatgagct tcaaaccaat caagtaaaat tgtctgcagg atattaatag ctgaaaacaa
    18061 tattttcaaa agaaaaaaga gaaggtgaca aaaattgtgt caccctctct ttttttattg
    18121 ttttttaaag tgataggttg gctcctccag gttgggatca taatcaacaa gtaaatcata
    18181 tcctttaaag aaccattcat ctgcttcctc gataaaaaaa agtgtcccat caatttccac
    18241 tttctttaca ggatgttctg gctgttcaac tgacattcca atcgaaaaac catcatgaac
    18301 ctctgttttg ccgtagactt ttccaaaaaa acgtattccg taagtttctg gcagatcaat
    18361 ttctgagtca aaccactttt gtgcttttgg cgttaccgtt aatttcatcc caacaaccta
    18421 ctttctcttt tctttctatt gattcatctt atcgtctaga atatccacat cgcctgtctc
    18481 tccaataacc agtagattat cattttcacg aacatattca tctgctgcag gagaagcgat
    18541 aaccgtttga tctactcttc gtatggcgac tactgttaag tcaaaacgtt gcctgaaatt
    18601 taattcagct aatgttttgt tgaaaaattt aggattggag actcggattt ctgctaaaga
    18661 atatttatct gacaactcta ggtaatctaa aatattcttt gacactaaat tatgcgcaat
    18721 ccttattccc atgtctcgct cgggatggac gacgcgatca gctccgatct tttcaagtac
    18781 tctggcatga tactcatttt gggcttttgc taaaatattt ggtacgccca tttcctttac
    18841 catcagagta actaagatac tggcttgaat atcttctcca atagcgacaa ttacatgatc
    18901 gaaatttctt atgcctaatg atcgaagtgt catttcatcc tgtgcatttg ccacaacagc
    18961 atgagtggct atattcatat attcattgac ccgatcttca ttactatcaa tagccagtac
    19021 ttcttgacca gcttcgatca gtgttcggca gatacttccg ccaaatcgtc caagacctac
    19081 aattgcaaaa ttttgtttca ccgttttcct cctttagtat cttcttttct cagtataaca
    19141 aagcctttac aaaaacaaaa gcaagcatca ttattgtaaa aaagccaaga aaaataatag
    19201 ttattttctc agcttttgat tatcctcttt atcatcgttt ttatttcacc aaaccatgtt
    19261 tgaatgcata gatagcggct tgtgtacggt cttccacttc tagcttagcc aatatattgg
    19321 aaacatgtgt ttttactgtt ttcagtgtaa taaacaattc gtccgctatt tcctgattac
    19381 tttttccttc agaaatcagc attaaaattt cattttcccg attcgttagt tcttcatgta
    19441 atacaggctc atttcgccga ctcatttttt ccatcatttt tgttgtcact tctggttcta
    19501 atactcgctc gccacgttgc gttgccctta tagcatcagc aatttcatgt gcggtagagg
    19561 tcttcaacaa ataccctgct gcacctgctt caatggcagg ataaactttt tcgtcatcaa
    19621 taaaactggt tacgataatg attttagctt ctggccaatc ttttaagatt gcttttgttg
    19681 attcgatacc atccatttct tccatgacta agtccattaa aatgacgtcc ggtcgaagtg
    19741 tcatcgcctt ctcatagcct tgtcgtccat tttcagcttc tccaattact tcaatgtctt
    19801 cttgaataga taaataaaaa gagacgccta agcgcaccat ttcatggtca tctactaata
    19861 aaacttttat catccttatc tatgcctcct tcatcaaagg gaccttgatt tctacacttg
    19921 taccttgacc tttaaaacta atgattttta ctgttccacc aataccagct actcgttctt
    19981 tgatattgtt caatccataa cttcccgttt tcgtttcatt catattaaac cctgttccgt
    20041 cgtcaataat acgtaacaaa agattattgt ctattttgtg caaatatacc tctaattcat
    20101 tggctttagc atgtcttaat gtatttgata acaattcttg aacgattcgg aacaagtgat
    20161 cctcaatgga actagatagt tttacatctt ctacatccca tttcagcgaa atctggattt
    20221 tattttgcaa ttccttcaat aactgctcga taccttgctt taaacttttc tcttctaaat
    20281 tgactggacg tagatgaaga agcagcgcac gcatctcaga ctgggaagcg ttgatgatct
    20341 ctgctaccat tttcaactgc ttgcgaaaca tttctggtgt ctcgcttttt tctgcttgct
    20401 ccgtcaatgc cgacatcatc atcattgctg caaacaattg ttgcgaaact gaatcatgca
    20461 gctcacgggc taatcgatgt ctttctaatt ctaaaatttc ttctttggtt tgtccatcta
    20521 catattgggg acggctcgtt acaatttgta attcactaga tacttcaatc attttttctt
    20581 ttatcttagt gatttcctta tcaatatctt tgatgtataa atcgtccgat gcacttggga
    20641 tcggaagaag caacaatttg gattcataat tgccagaaga aagtgcacgc agcttttctt
    20701 cgattttccc gtattttgtc ttttgaataa gtgataataa tagaaagaca attaatccta
    20761 cacctaagga aatggctatc aaatggaaga taagcggaac atataataaa cgtgcacgca
    20821 gtaattctcc ccaccaatga ctttgattgc tggcataaaa ataagtgaaa agtgaaaata
    20881 gaataataag gaaagccgca atccccgaat aaacagctag catcgctctg gatatttttc
    20941 ccatcatatt cgtatcacct cgacatctcc aatcaaggta ttagtaataa tcttcaatct
    21001 acgagggttt tcatcataat cactgctgta gattttgatt tgttcatttt tcaaagaaaa
    21061 ttgctcttct tcaaacaaga catttcctac taatgtcgca tgctctaatc ggatggctac
    21121 gcccaaaggt actaagatcc gtgttctgcc tatccctttt cttacaatca caatattatc
    21181 atcttttggc aaaagcgtat tgcccaaatc tatgatagta tcaccagaaa tgacggcgat
    21241 gttgatatcg tcccattcat agacgtcgtt tccaatacgt tggttaccga ataattgctg
    21301 tcttttccgc tcattattat gtgtttttaa ttgctctgtt tggatcatca tgatctgttt
    21361 ctttcgccaa aaagcatttt tggttaaatc tatccctgaa atctctacac ctttcagacc
    21421 aataaataaa acggcaaata cagccatcat ccataaagca ggactgttga ctaagctaaa
    21481 aaaaatgacc aaacttccta atatcaattg aaaattttga aatttagtac gaggatgttt
    21541 gcgcatagct agatatatat tgaaaatacc gaatatcact aaaagcaaaa gtccagtatt
    21601 gtttacgatt tgccaaacag caaataacag cagtagtgct tcaactacca caaaaaaacg
    21661 ccaagaacta ttcatcgctc tttctccctc ctgacacatt ctcatttcat catacctatt
    21721 tttttaactg ttggctatag gactttagat gtaagatcgt ttgttgaaat ctaaaataca
    21781 caaagagaaa aagatctaga gtgtatcatc agtaagatga actctctaga tctttcagtc
    21841 agactacgct tgttctactt tgacgatttt aactaacata tcgccacctg gagtagcaat
    21901 agttacttca tcatttttac gtttgccaat caatgcttgc gcaataggag aatcattgga
    21961 aattttgcca gaaaacggat ctgcttcagc gcttccgaca atcgtgtact cttcttcttc
    22021 tccatctggt agttcgatga atgtaactgt tttaccaatt gatacctcat ctgcatctac
    22081 accgtcgtta tcgatgattt gagcaaaacg aatcatattt tcaattgttg tgattcttcc
    22141 ttcaacaaat gcttgttcat ctttcgctga ttcatattca gagttttcgg aaagatctcc
    22201 gaaaccgcgg gcaattttga ttcgttcaat gatttctccg cgtttgactg tttttagttc
    22261 ttctaattct ttttctaatt tttctttacc ttctagagtc attggataaa ctttctctac
    22321 catgtgtaac gtctcctttt ttcgataaaa taccgcaaaa ggtaatcttt tgaggaatat
    22381 agtccaaaaa agattaccac gcttttcttc aaatgtaaat gtttttctaa aaatgatcaa
    22441 tgtctagagc ctgtttttct aagaattatt tacatatttt tccactaatt ccatatgttc
    22501 ttcataagtt ttagcgaaat aaacatttcc agtgctcgta tctgctacga aataataata
    22561 gtcattttct gctggttcta atactgcctt gattgcctct tcgcttggat tgtcaaaagg
    22621 tccaggtcca tagccggtat tggtatacaa attatatggt gaatctacag ctgtatcttc
    22681 atacgtcact aattctttgt gttcacctaa tgcgtataaa acagagatat ctgactgaag
    22741 tggcatattt gctttgattc ggttaaagaa gacttgtgca atgtttttac gatcattttc
    22801 ttttacgcct tctttttcaa ctagagaagc tagagttaaa acttcttgta cagtcaaatt
    22861 tttctgcttg atagctgaat aatagttttg catgaccgta ttcgtcttgt tcaccatttc
    22921 aatcactaga tccttaagcg aagtactcgt gtaataatca tatgtggctg ggaataagta
    22981 accttccaaa cggtattttg tgttctcagc tttagatgca ctttccaaca gttctgggaa
    23041 tttttggtgc aactcgttaa agaaagtctc atcattcact aaatctaaaa attctttctt
    23101 gtctttacca gttaccttcg ctacccgttc agcaatctga tcgatatcgt acccttctgg
    23161 aatggcaatt ttcccatctg caacttttgt tggctcagaa gtaccgcctt cttgcaactg
    23221 tttgccaatc tcgtcaagag tcatattagg cgctaactga taatagccag cttgaaagcc
    23281 agttaaattt ttaaattttg tataataatt gaaaacaatc ccgcttttaa taatattgtc
    23341 cttctccaaa atctcaccaa tctgcttgtt agaagagcca ctcgggatct cgacttggac
    23401 taattggtca tctgatttat ctaaaggctt caacccgcta tctacataac gatacacggt
    23461 aaatccaaga aatccgccaa taatcaataa tgtaagagca atcactaaaa tgatcttcct
    23521 cacaatctta tcttcttttt tacgcagatt tctccgcttg tcgattatat ctgctgtagc
    23581 tgccttactg gcttcttttt tatctttact tgggtgttgt ttgtttcctg acaaaacaac
    23641 tcctccttac tatcaagttc gtaactttat tcattataca tgatatttga attggcgtga
    23701 atgattttat ttaaatattc tatcaaaaat gtaagtagta aatccattac tataagatta
    23761 aaataaatgt tatcaagata caagctattt tagctttatt ctttctgtaa aaaagaaagg
    23821 agactaaata aactattatg aaaaaaggaa aacatgctaa aaaaagaaaa tattcatttt
    23881 taatgatcgt tgtcgtgttg tttagcctta gtgcgttctc tacaggaact attcttggat
    23941 ggcaatttta caataatgaa cactctcgga tagttgccca tgctcaatca gctcaaaaaa
    24001 agttgatact gactaccaat gaacaacagc tcgttgaaaa aatcgatcaa ctattaagaa
    24061 aaaatgatta tattggctca atttacgtaa agaaaaataa tcgcatcatc atggaaaaag
    24121 gctatggcta tgcgaataaa ctcacgcaac aaccaaatga tccttctctt tattatcaga
    24181 ttggttcggt tcaaaaagca ttgactactc ttttgatttt aaaacaagtc gagcgtgggc
    24241 acctttcttt agatactaag ctggctcaat tctatccaca aattgctggc agccagcaaa
    24301 ttactatccg tgacttgctg tatatgcgct caggcttgca ccgaactgct tctccaacca
    24361 taccgatgac tgatgaagaa gtcgttcaat ttgcgattca gcacttaaaa tttacagatt
    24421 atcagacctc tcattatgat tcattgaact ttaccatatt gactggaatt ttgatcaaat
    24481 taacaaatca accgtatgaa aaattattga ccgatgatat catcgaacct ttaggattac
    24541 agcatacaaa attttatgac caagtcaaaa attcatccaa tcatgcacta tcctatcaaa
    24601 tgtctaaaga ggatgattac tttaaaccac tgacagaatc tgaaacagag atccataacg
    24661 aattagggac tggtaatatc agtatgtccg tctatgattt aaatacgctg tttacacaag
    24721 ttttatctgg aaaactagtt tccaaagact tactcttctc attatggcgt gaaaatgcga
    24781 atggacatcc atatagtggt ggggtatata gcggagataa ttatatcctg tcgcaaggaa
    24841 atatcgactc ttttgataca tctgctatat ttagcaaaga ttcacaggat gctatcatca
    24901 tggaaagcaa tataaaagct gacaaaacta tcaaactgcc tactgctgat ctaagaaacc
    24961 aaatctatga cctgatggaa ggaactacta agttggcaaa tagctgatat gatgaaaaga
    25021 tagcaaatac tacttctttg aatagcattt gcttaaaaaa agtactcgac taaacgtagg
    25081 aaagagtgtg ggacaaatct aaaaatcaat ttttgctaca gactttaatt ccaaataaac
    25141 agaacagaaa gaggt
//
LOCUS       DBNICI010000026        24597 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.026, whole genome shotgun sequence.
ACCESSION   DBNICI010000026 DBNICI010000000
VERSION     DBNICI010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.026"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(13..996)
                     /locus_tag="AC1ZCC_000854"
     CDS             complement(13..996)
                     /locus_tag="AC1ZCC_000854"
                     /EC_number="1.6.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HIQ1629126.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(1001..1648)
                     /locus_tag="AC1ZCC_000855"
     CDS             complement(1001..1648)
                     /locus_tag="AC1ZCC_000855"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HIQ1629127.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1733..2365)
                     /locus_tag="AC1ZCC_000856"
     CDS             complement(1733..2365)
                     /locus_tag="AC1ZCC_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIQ1629128.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2381..2932)
                     /gene="coaC"
                     /locus_tag="AC1ZCC_000857"
     CDS             complement(2381..2932)
                     /gene="coaC"
                     /locus_tag="AC1ZCC_000857"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HIQ1629129.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(2929..3666)
                     /locus_tag="AC1ZCC_000858"
     CDS             complement(2929..3666)
                     /locus_tag="AC1ZCC_000858"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HIQ1629130.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3663..4652)
                     /locus_tag="AC1ZCC_000859"
     CDS             complement(3663..4652)
                     /locus_tag="AC1ZCC_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIQ1629131.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            4910..5452
                     /locus_tag="AC1ZCC_000860"
     CDS             4910..5452
                     /locus_tag="AC1ZCC_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1629132.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            5469..6554
                     /locus_tag="AC1ZCC_000861"
     CDS             5469..6554
                     /locus_tag="AC1ZCC_000861"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1629133.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            6566..7372
                     /locus_tag="AC1ZCC_000862"
     CDS             6566..7372
                     /locus_tag="AC1ZCC_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1629134.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            7373..8200
                     /locus_tag="AC1ZCC_000863"
     CDS             7373..8200
                     /locus_tag="AC1ZCC_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1629135.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            8197..9270
                     /locus_tag="AC1ZCC_000864"
     CDS             8197..9270
                     /locus_tag="AC1ZCC_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIQ1629136.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            9698..12394
                     /locus_tag="AC1ZCC_000865"
     CDS             9698..12394
                     /locus_tag="AC1ZCC_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIQ1629137.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            12459..12959
                     /locus_tag="AC1ZCC_000866"
     CDS             12459..12959
                     /locus_tag="AC1ZCC_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIQ1629138.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            12971..13465
                     /locus_tag="AC1ZCC_000867"
     CDS             12971..13465
                     /locus_tag="AC1ZCC_000867"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIQ1629139.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            13486..14295
                     /locus_tag="AC1ZCC_000868"
     CDS             13486..14295
                     /locus_tag="AC1ZCC_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIQ1629140.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            14292..15113
                     /locus_tag="AC1ZCC_000869"
     CDS             14292..15113
                     /locus_tag="AC1ZCC_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HIQ1629141.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVMGILLSLLGIA"
     gene            15164..17263
                     /locus_tag="AC1ZCC_000870"
     CDS             15164..17263
                     /locus_tag="AC1ZCC_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HIQ1629142.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            17260..18378
                     /locus_tag="AC1ZCC_000871"
     CDS             17260..18378
                     /locus_tag="AC1ZCC_000871"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HIQ1629143.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            18417..19295
                     /locus_tag="AC1ZCC_000872"
     CDS             18417..19295
                     /locus_tag="AC1ZCC_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIQ1629144.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            19441..19638
                     /locus_tag="AC1ZCC_000873"
     CDS             19441..19638
                     /locus_tag="AC1ZCC_000873"
                     /inference="COORDINATES: protein motif:HMM:NF016526.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HIQ1629145.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            19622..19852
                     /locus_tag="AC1ZCC_000874"
     CDS             19622..19852
                     /locus_tag="AC1ZCC_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629146.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSILIDNKLR"
     gene            20097..20288
                     /locus_tag="AC1ZCC_000875"
     CDS             20097..20288
                     /locus_tag="AC1ZCC_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629147.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
     gene            complement(20662..21474)
                     /locus_tag="AC1ZCC_000876"
     CDS             complement(20662..21474)
                     /locus_tag="AC1ZCC_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1629148.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            21637..21744
                     /locus_tag="AC1ZCC_000877"
     CDS             21637..21744
                     /locus_tag="AC1ZCC_000877"
                     /inference="COORDINATES: protein motif:HMM:NF013540.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1629149.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            21802..22869
                     /locus_tag="AC1ZCC_000878"
     CDS             21802..22869
                     /locus_tag="AC1ZCC_000878"
                     /inference="COORDINATES: protein motif:HMM:NF014537.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HIQ1629150.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            23011..23214
                     /locus_tag="AC1ZCC_000879"
     CDS             23011..23214
                     /locus_tag="AC1ZCC_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HIQ1629151.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            23274..24476
                     /locus_tag="AC1ZCC_000880"
                     /pseudo
     CDS             23274..24476
                     /locus_tag="AC1ZCC_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         7636 a         4197 c         5225 g         7539 t
ORIGIN      
        1 atcagcatga ttttattcag ttatcttcaa aatgattttt ccgatgtttt tgtttgcttc
       61 catatattca tgcgcttgtt tgactgcgct gaattggaat gtacggtcaa tgattggccg
      121 aatcttgcca ttttctagaa aaggcccagc tttttgcata aaagtttgtg cgagctctgc
      181 tttatactga tcacttctag gtgtcaataa tgtaccaagc agttggacac gtttcccaat
      241 caatacacca aggtctatct caggcacgat ggtcccacct agcataccaa tcaagaccca
      301 acgtccatca acggctatag aagctaaatt cttttcccag taagaagcgc cgatgaaatc
      361 caaaataaca tcgactcccc gaccttcagt gatttttcgt acctcttctt gaaagtcttg
      421 ttttttatag ttgatcaatt catctgcacc aagttcttta caaaaggcga gtttttcttc
      481 tgatcctgca gtaacgatga ttttcgcgtg agaaagctgt ctagctaatt ggatcgctgc
      541 tgttcccaca ccacttgctc ctgcatggat caagacagtt tcgccttctt gtaattttcc
      601 aagccagtaa agtgtttgat aagcagtcaa aaaaacttct gagattccag cagcagaaac
      661 atagtctagc gtatcaggga gaaggattgc acgatcggca ggcatcacag catattcggc
      721 ataaccacct agattgacta atccgtatac ttttgtccca ggagtaaaag tggagatatc
      781 acttttattc tcaactacct ctccagctac ttcgactccc aaaatagggt agggaggttt
      841 caaagagact gattctcttt tcataatatc tgttcgattg acagctgctg tatggacttt
      901 gatcagtaat tcgccttgtt ttggtttagg aacaggcact tcaatttgtt ctaatacatc
      961 tacagaacca ggatgcttga ttgaaatggc acgcatggat ctattcctcc tttgcccatt
     1021 cttggaatgc tgtattcaac tctttgctac cttcagaaag tactagagtc gtacgagaaa
     1081 tatccgtttc ttccactaga gcggcaagta cagcagcaac tgtagagcga gtgatcgttt
     1141 ttgatgcctt ctcattaggc ttagttaatt gaatggaagt aacttcttca tcgttagtca
     1201 atgtgactgg ttgaagaatg acaaaatcta agttagttcg tttcagccat tcatctgcat
     1261 aatgtttagt aatataataa tctgtcatgg actcaggcca ttttgctggt tcatctgcat
     1321 aaacagcact gaccatcaga tagcgagaga catgcgcatt ttcagctgcg atcactgttt
     1381 tgattgcgcc atccaagtct acttgcagaa gattctttcc ttgagagccg gcagcaaaaa
     1441 tcaatacatc gatgcctttg aatgcttcgg tcattttttc cacggaccaa gttaaatcaa
     1501 aagagacata cgaaacattc tcttttttta ctaatgtttg ggaagcaaca tctctcaccc
     1561 ctgcaaaaat ttcatgggaa cttggagcta aatcttttat caggtgtcgg ccaatttgtc
     1621 cgtttgcacc tacaacaaaa attttcattt ggatcatcct ttcgacgtca aatctttcca
     1681 ttcaatagta acaccaaaag agaaaaaaat cagagatcgg ctacgatgac tatcagttag
     1741 taagagaagg gtgcttgaag tatcttaaca agacattgca tactgctgca gtcgcaagac
     1801 ctgcgacgat tccttcaggt ataccacttg taaaaataac cgctagaata gctgaataaa
     1861 cagcatttaa atttgttccg atgactttgg cataatcttg ctggaataaa aagtagatca
     1921 tattcatcac taaaatcgta ttggtcaatg aaccggaaag tccagctaag aataaagaaa
     1981 cggaatctgc tttttgtttc attagtttgc gcagaccttt ataaacgaaa taaggaacca
     2041 ccccaatcaa gatgcgagga ataaacgcga tcagcaaggc tttccagctt ccatgttctg
     2101 ttccgatgac tgggataaac ggagaaaaga caaaagacaa aggtgtttgg ataactgtgc
     2161 tacggatcat gctgatcagt ccaaatgtac cgccaagaaa agcacctagt cttggtccta
     2221 aaacgatcga agcaatgatt acaggaatat gcatggttgt tgcattgatt ggaccgatag
     2281 ggataaaacc taaaggcgta acagctaata aaatcaaaat agctaaaaac atcgcagtta
     2341 atgtaaagtt ttttgtattt ttcataagta ctcctttttt ctaagcattt ctttcattaa
     2401 gagtcagcat gacctgttcg atgatcgtct gaatatcagc taaggcacct tttccaaaat
     2461 ctccacaagc taaaagagat tctcttggtt caatttcctg atagccgaca gatctcaatg
     2521 tttccagatt acgctgagtg atcgggtttt gatacatatt cgtgttcatg gcaggggcaa
     2581 tcaattttgg tgttcccact ggaagcgcca ttgcgactgt tgaaagaaga tcgtctgcaa
     2641 tgccgtttgc tagcttgccg ataatgtttg ctgtagcagg cgcgatgaga aacaagtcag
     2701 cttgttttgc tagttcgata tgattgatta cctgcggatt tgattcttgc atgacgtctg
     2761 tatggactgc gcgcttcgac agagattgca aggttaatgg cgtgataaaa tgtgtgctgc
     2821 ttttagacat aatcacatct acctgggcac ctaatttgac gaattgactt gtaagatcag
     2881 cgcttttgta agcagaaata cttccagtaa cgcctagtaa gatctttttc attttgattc
     2941 ctccagtcta attttctcag taatcaatgc agcaatctca gatttacttt gagcttcttc
     3001 cactgttcca tctttagata agagatagcc gtggtgtgtc tcgccatgga tttccgtcaa
     3061 atcatttgcg aaaataaaat cagttttgtt atttatcaga ctattttttg cgactgccag
     3121 aagagattcc tttggtacat ctactaatag tttgaaacca atgatttttg cttttggctg
     3181 ccattcacgt agataagaaa tgatttttgg tgttttcttc aaaacgagca caagatgctc
     3241 ggtgttagaa gagatctttg aaacagtggt atctgtctgt tcattttccg caaaccaatt
     3301 gtccagttct tcttgcgttg aagaagtggg taggtttttt gccagctgtt cttctgaaaa
     3361 actaaaagca ggagtaaaat cactgactgc catactgtgg ataatcgcat cgtagtgttc
     3421 ttttttcgtt agttcttcta attgtaaaaa taagtccagt gcagattcga tcgtataaat
     3481 cgttatattg gagtgtcttt tcggtttcag tgcttgtttc gtagtcacgt aatcgattgt
     3541 ggtatccgga ttggcagcta aataatcagc taatgcttgc cctaatcgtc ctgtcgaatg
     3601 atttgtaata gcacgtactt gatcgatttt ttcagtcgtt cctccagctg tgattagaat
     3661 cttcatttat ggtcacctac atttactagt gttcttcctt ggtgtgttcc agcaagtagt
     3721 ttagatgctg tttcaggcaa ttgatccaga gtaatctctt ggacgaccag ttcatctgca
     3781 atctgcaaat ccgccatctg ttgccataaa gaaagacgtt gatctattgg tacattgaca
     3841 gaatcaattc caatcaactg gatgtttcgc agaataaaag gcaagacagt cgcgtttagt
     3901 ttgattcccc cagcattgcc acataaagct actgcaccgt tataagagat caatggaaga
     3961 agactgctta gctgctcgcc acctactgtg tcgatgacat agtcgatttg ctgttttcct
     4021 aaagcttttg ttgtctctgg cagaaattca tcaggtcgta cgacttgcaa agcacctaga
     4081 ctttttagcc atgtgacaga ctcttctttt ctggaaaagg caatgattga ctggaatcct
     4141 agtttatgta acagggctat cgctgtactg cctacaccgc cagaagcacc agtaacgaca
     4201 acttttttat cggaggtcat accttgacgg attaaagcgt tcacacacaa catagcagta
     4261 aaccctgctg taccaaggat catcgcttga cgcagcgtca tattttttgg tagaggaact
     4321 aaccagtctc ctggtacttt ttgatattgg ctataaccgc ctggatgggt gacgccaagc
     4381 ccaaaaccag tagcaagtac aagatcttct tttgaaaaac gtggattgtt actttggacg
     4441 acagttccgg ataaatcaat ccctggagtc atcggatatg ttcgtatcac ctgaccagac
     4501 tcgctcattg ccaatgcatc tttataattg acatcagaat aggcaatttt taccaagact
     4561 tcattttcag ccaactcagt aaatggcaga tcactgattg ctgtatggaa aacaggttcc
     4621 tttattacct gaaaagatcg aaaagcgttc attcgtgaga cactcctttt aaaattttct
     4681 tttatcatac cataaagcct actcttttat tcttgttctt ccgtaaattt agacaaaaat
     4741 taggtgaaaa ataaagaatc aaacaactag aatttattcc aaaagataaa gaaatattca
     4801 gcaaaaaaaa ttttacaaag ggttgccttt ttcaataaat ttcgtatact aatacgagtg
     4861 gtagtaaaca ggaagtttac tattagaaaa cttcgattcg gaggaaatta tggaaattgg
     4921 tgagaagtta agaaatttgc gcatccaaaa gaatttgaca caagaagaac taggcgaacg
     4981 cacagatttg tcaaaagggt atatttccca gttggaacga gatttaagtt caccatcaat
     5041 ggaaacattc ttttctattc ttgaagtctt aggtgtgact ccagaagaat tttttcatca
     5101 agaagcagcg aatctgcaag tggtttattc aaaagaagat catacggttt atttcgatga
     5161 agaaaacggg tatgaattgg aatggctcgt ggctgattcg aatgaaaaag aaatggagcc
     5221 tgtcttgctg acttttgaag gtgcaggaga atataagacg tttgaacctt cattatctga
     5281 aacgtttatt tttgtattag aaggggaatt agagttaagc ttaggagaga cggtctatgt
     5341 ggcaaaagca ggacagtcga tctattatca agcgacaaaa caacatcaat taaggaatca
     5401 tctcaaaaaa cgaacaaaag tattgattgt tgcaacgcaa tcatatttgt aatatagggg
     5461 gtaaaaacgt ggggaaacca atcatttcat ttgatcaagt agtgaaacgc tatgatgatg
     5521 aaacagtatt aaaaaaagta agttttgaga tcgaacaagg aaaattttat acactgcttg
     5581 gtccttccgg ctgtggtaaa acaacgatcc tacgtattat agctggattc accgaagcta
     5641 cagaagggga catctatttt gaagggaaac ggttgaatga tttgccagct aataagcgac
     5701 aagtaaatac agtcttccaa gattatgcac tttttcctca tatgaatgtt tttgataacg
     5761 ttgcttttgg attgaagatc aagaaaatgt ccaaatcaga tatcgaaaag aaggtaaaag
     5821 atgctttgcg tatggtccag cttcctggat acgaaaaacg ggagattagt gaaatgtctg
     5881 gtggtcaaag acagcgtgta gcgattgcac gagcaattgt aaatgaaccg aaagtattgc
     5941 tattagatga accgttatcg gcgcttgatt tgaaactacg tacagatatg cagtatgaac
     6001 tgcgggatct gcaacaacga ttaggaatca cgtttatttt tgttactcat gaccaagaag
     6061 aagcgttggc aatgagtgat gagatttttg tcatgaataa agggaaaatc gtccaaagtg
     6121 gtacacccgt agatatttat gatgagccaa tcaatcattt cgttgctgat tttgttgggg
     6181 agagcaatat cgtggacggt gtaatgatcg aagataatct agttgagttt gttggtaagc
     6241 aatttgaatg tgtcgatggc gggatgcgtc taaatgaacc agttgaagtt gttttacgcc
     6301 cagaggattt gacgatcacg acaccagata aaggaaaact agtcgtaacg gtcgatactc
     6361 agctgtttcg tggtgtccat tatgaaatca tctgttatga tgaacagcaa aatgaatgga
     6421 tggtccattc aacgaaaaaa gctaaagaag gaagcaaagt aggattggcc tttgagccag
     6481 aagatatcca tgtcatgcgt ttcaacgagt ctgaagaaga attcgatgca cgtcttgaca
     6541 gctacgaaga atagggggga caagaatgaa aacaatgcgc aggatctatt ccgtccctta
     6601 tattttgtgg ctcggtttat tcgtgatcgc gcctgtatta atgatcattt atcaatcttt
     6661 ttttgatatg aatgggcaat tcacgctgaa taattatcta tcttatttta cgtcggggac
     6721 atcgctttct atgacgatca attctgtatg gtatgcattt ttgatcacgt ttttcacgct
     6781 gatcatcagt tacccgactg cttatttttt aacaaaactg aagcacaagc aattatggct
     6841 gatgctgatt attttgccta catgggtgaa tctgctgctt aaagcttacg catttattgg
     6901 gattttcagt gtgcacggca gcgtgaatca tttctttgat tttatcggga tcgggccgaa
     6961 acagatatta ttcacagact tcagttttct gttcgtggca acgtatatcg agattccatt
     7021 tatgattatg ccaattttca atgcattgga agagatgaat ccttctttga tcagtgctag
     7081 tagagattta ggcgcgaata gctttgagac cttccgacga gtgatttttc cgttatcatt
     7141 aaatggtgtg aaaagtggtg tccaagcagt atttattcct tctctttctt tattcatgct
     7201 gactcgtctg attggcggaa atcgggtgat cactttagga acggctatcg aacagcattt
     7261 cttggttaca caaaactggg ggatgggttc tacaatcggt gtgattttaa tcattgctat
     7321 gttcatcgtg atgctcctga caggcgagaa gaaaaaagga ggtcgctcat aaatgaaaaa
     7381 atttaaatgg tcgtatcttt atttagggat cgtcttttta ttcttatatg caccgatctt
     7441 ctatctgatt ttttattcct tcaatgaagg aggaacgatg aacagcttta caggatttac
     7501 ttgggaaaat tatgaagctg tttttgaaga tacacgtttg atcattatcg tattgaatac
     7561 gttcatgctg gctttcttat ctgcattgat cgccacgatc atcgggacat ttggggctat
     7621 gggaatctat tacacgaaaa aacgacgtgc acggacagcg ttgttgagca tgaataacat
     7681 cttattagtc tctcctgatg ttattatcgg tgctagtttc ttgattttct ttacactact
     7741 aggattcgga ttaggttttc agagtgtatt gctttcccat attgctttca gcattccaat
     7801 cgtcgtcttg atggtcttgc caaaattgca ggaaatgaac gattcaatgg tcgatgcggc
     7861 tagagacctt ggtgctaata atttccaagt agtcaaaaat atcattttgc cttatctttc
     7921 tccaggaatc atagctggat attttatggc atttacctat tctcttgatg attttgcggt
     7981 tacatttttc gtcacaggaa atggctttac gacgctctca gtagagatct attctcgtgc
     8041 aagacaagga atcagtttag aaatcaatgc gctaagtgcg ttagttttct tgttctccat
     8101 gatattggtc attggatact actttattag taaagaaaac cgtccaaaac ggctgaagaa
     8161 aaacagaaga gtacgcgagg gggtggcacg gctgcgatga aaaaattaca atctctgttt
     8221 ttaggtattc tcatcattat tgcggtgctg ctgcttggtg ttcgtcaact tgaaaaaaca
     8281 agtggcatga gtggagcaaa ggtacttacc atctataact ggggagatta tatcgatcct
     8341 agtttgatca cgaaatttga aaaagaatat ggatacaaag tgaattatga aacatttgat
     8401 tctaatgaag cgatgttcac aaaaatccag caaggtggga caaactacga catcgctata
     8461 ccaagtgaat atatgatcca aaaaatgatc aaagaaaaac tagtacttcc attagatcat
     8521 tcgaagatca aaggattaaa taatattgat ccacgatttt tagacttgga ttttgatcca
     8581 gataatcagt attcgattcc atatttttgg ggaactttag ggatcgtata taacgataaa
     8641 ttctttgatg caaatcaaat taagcattgg gatgatttgt ggaagccgga attaaaagac
     8701 agtttgatgc tgattgacgg tgcacgagaa gttatggggc tttctttgaa tagtttaggc
     8761 tattctttga atagtaagaa tatggcacag ctgacagaag cagcaaataa attgaatcat
     8821 ttaacaccaa atgtcaaagc aatcgtggca gatgagatca aaatgtatat gatcaatgaa
     8881 gaagcctccg tggcagtgac attttctggc gaagctgccg atatgatgtg ggaaaatgaa
     8941 catttacatt atgtgatccc accagaagga tccaatcttt ggtttgataa tatcgttatg
     9001 ccaaaaaccg tgaaaaataa agaaggtgcc tatgatttca tcaactttat gctagaaccg
     9061 gaaaatgcgg cacaaaatgc agagtatatt ggttattcta caccaaatga agaagcaaaa
     9121 gcgttattgc caaaagacat ttcgtcggac gaacaattct atccatctga tgacacgatc
     9181 tcccatcttg aagtctacaa agatctagga tcgaaatacc taggcatcta taatgaccta
     9241 ttcttagaat tcaagatgta taggagataa tggcaaaact cagtaagaat ggggacttat
     9301 caacgttcta aagagctaaa aatcgtcctt tttctatctg agttttccaa tcttctacca
     9361 aaagcggatg aaacgccgct ttttttgtgt tattttattt tttctaccac gtttttctac
     9421 cagagaaaag tggacaaaac cattgtaaaa tagggcccga gacgggctag taaatctaag
     9481 attgaaaaag ttacagaagg ataaagtaat tggaaactat gcaaatatac ttaacccttc
     9541 tgtaatctat taataaaact aatatatgaa tccagttcaa tatttgtagc tatgaaaaaa
     9601 cataaaaatc atattagata aaaactaaat tggtagataa attattttgt cagatatctg
     9661 aggcaaatat tcgactattt gtgtttcaaa gtaaaaattg gaatacaaat atagaataga
     9721 catattgcag ccgatagttt attatgcttt tagacacatt aaactggagg aaaaaatgaa
     9781 aaagagcgct aaagatcgag tttttgatga gctgatacga ctaacaggga atatgtcttc
     9841 tgtcactgcc gagaaaattt cgaagaatct aggtatctct cgtcagaatg ccagccatta
     9901 tttgacgaga ttagtggaag ataaaaaggt cgagaaatta ccgggaaagc ctgttttgtg
     9961 gaagccttta gatgaatatg cggttgtaga taatacggag caaataaacg aagcctttca
    10021 ttctgtagtt ggtcatgacg gaagtctgag agaggtcatt caaaaatgta tagccgctgt
    10081 caaatatccg cctaacggac tcagtgtatt aataaatgga gccacgggtg ttggaaaaag
    10141 ttttttagcc acaaaaattt ttgaatacgc tatccatgag caaattattg aaaaagatgc
    10201 accatttgct atcttaaact gtgccgatta tgcggacaat cccgaactgc tttcggcaac
    10261 actgtttgga tacaagaagg gggcctttac gggggctgaa aaggataccg atggtttatt
    10321 ggcaacggcc aacaacggct atctttttct cgatgaggtc catcgcctat cgaaagaaaa
    10381 tcaagaaaag ctctttctat ttattgatac tggaaactat cgtccagtcg gtgaaaatgt
    10441 aaattggcat tctgctaagg ttcgttttat tttcgccacg acggaaaaag gtgaaaatta
    10501 tttattagac acctttgatc ggcgtattca aattagtgtt atgttgccaa cttttgaaga
    10561 tcgccccatt cgagaacgat tagagctgat tcaattattt tttcaaaatg aagcggatgt
    10621 tttacaaaaa gatattattg tttcaagaga agccttgtct ttaatgttat ctcatccctt
    10681 tagtgggaat attggtaagc tgaagaatat cattaaaatc agttgtgcgg atgtgtatag
    10741 tcgaacacaa gaagaaccat taacgattgg gaaaaatgag atcttgattc agttaaatat
    10801 gctggaatcc gaagtagaaa gcttgccgat tgctagttta ttgataaaga aaaatcaaaa
    10861 gataaaaacg gatcgaatca aaccgtttga ttatgattcg attttggaaa aaattactca
    10921 gctgataaat gaaggtaatt tcaatgaaaa tttttcttcg ataaagaatg aagtacagca
    10981 tttggtgcat cacattgata aggattcttt tttatcaatg agtagaaaat tgatgattca
    11041 gatgtttaga aaggtctggg tatccgtttt aggaaaaacg tacggattga cgacgagtgg
    11101 acctgttgca gaaattatta gcagatccta tgtcatttcc ccaagctata agacagactt
    11161 aaccgggata aatcgtacat tagctgcaaa tctacctcgg acgacttact tatgtaatca
    11221 gttcttggag catttaccac cgctctcaga aaaggacgag aactttctaa gactgcttat
    11281 tgtcgtgact ttatctgatt atgtagatga atccatcgag ctgaagggcc tgttgcttgc
    11341 tcacggagat agtacggcta gcagcattca agcagtagtg aatcaattat gcggcaatta
    11401 tgtttttgaa gcgttggata tgccagtaga gagtaatgtg tcagagattg tcgaatcgac
    11461 aaggaatttc gtgaaacggc aagtgaatac agatagtcta gtgctgatcg tagatatggg
    11521 ctctttaaat caaatttaca cacagatcaa gaacgattta aaaggggagt tacttcttct
    11581 gaataatttg acgacttcca ttgcacttga tattggcttg aagatgacga tgaatgcgcc
    11641 atttaaacag attgcggagg atgcagcaga caagtattca atcaatgttc aatactttga
    11701 aggcttttct caaagtagca acattatcat ttcctgtatg tcgggtttgg ggatttcgga
    11761 caaattgaaa gatgtttttc gtcatcattt atcggatgaa tccatcgaag ttttcacgaa
    11821 ggactacaga gaactacgtg aattaatcga ccaaaatgat gaaacttatt ttgacaagac
    11881 aaaattggtc atcacgactt cggatcttcc acgttccttt tcaattccta atattaatgt
    11941 ttatgacatc ttagacggtg atggagcagc caatttggag catattttga gtcctgaaat
    12001 gaagccggca gaatttaacc gcttgattca ggagctagta aaattctttt cgttggaagg
    12061 aattgcggac cgtttgaact ttctgaaccc aacgatcgtg atgagtgaag tggaaacagt
    12121 catttctaaa tatgaaaatt actatcattt cactttaaat ggccgtgtaa aactaaatct
    12181 ctatatgcat acggcattga tgatggaacg tttattcttg gctcggacag aaaaatctga
    12241 agaaccgaat tatgatttga aaccagatga agatgaattt tattatgttt ctagaagtat
    12301 ttttcaatca atggagatga aatataattt taaagtcaat gcttatgagc tttcgctgct
    12361 ctatgaatta ttaggaccgt atattcaaaa ataaagtgtc taaaaaagtg tctaatctgt
    12421 tttttttagg ttagacactt tttttaatgt gaattaacgt gattaaacca atgtttttaa
    12481 aagttggcac aggaattgct tatataaaag tgaacagaaa gaaaggaatt aaccatatga
    12541 agaaaattct aatagcgact cacggtcact tagcttcagg ctttcttagc agtattcaat
    12601 tactgacggg gaaggttcaa gagattacgg tgattaatgc ttatgtggat gagtgtgatt
    12661 ttgaacagga gttgaatgta tttgtaaacc aggtgaacga gcaggatcag atttttgttt
    12721 ttaccgattt atttggcgga agtgtcaatc agaagattac caaaaccttt ttggagaaag
    12781 agattgctgt aactattttg tctggcttta atctgccaat tctgctcgaa atagtattgg
    12841 caaccaagga attaacaaag gaggatattc aggaactggt aaataaatgc caacaggaat
    12901 taaagatgac cattcttgat gaggtacatg tagtaaatga agaggaagat tttttttagg
    12961 aggaagaaaa atgattaaag cagtgcgaat tgatgaacga ttaattcatg gacaagtagc
    13021 gatgacttgg acaaagagtc taaatttaac cgggttagta gtagcaagcg atgaggcggc
    13081 taccaacgat attcaaaaga tgacattgaa gatggctgca ccatcgaatg tcaaagtcat
    13141 tattaaaaca gttgatgacg cgattcattt gttaaaagat ccgcgggcga aggacatgcg
    13201 cttgatggtt ttagtcccta atgtcaaaga tgcggtaaaa gttgcgaaag ctttcccaac
    13261 ggaaatcgaa ttaatgaacg tggggaatgc tgggaaaatg acaccgccat cggaagataa
    13321 gaaaacctta tcaaaggaag tgatgttaac gacgtcagaa attcaagact taaaagaatt
    13381 agttgaactt tatccagata ccgtttttca gcctacaccg gcgatggaaa aacgttcagc
    13441 tatctctatt ttgagtgatt tttagaaaaa ggaaggagaa aaattatgta tgatgcattt
    13501 gtagtcgctt tggccgtttt tattggagtt gctggacatg aaatgttcgg gatgtccatg
    13561 ctgagtcgtc cgattgttgt tgctccgtta gcaggagcgt tgatggggga tttgcagacg
    13621 gggttgattg tcggggcgtc actagaagcc atctttatgg gtgtcgtcaa tatcggaatc
    13681 tctagtacgg cggagccttc tttggctgct gggctggcta cagcgttcgc aatcaaaatg
    13741 ggaggaaacg tggatgcgat tattcccatt gtctttccac tagcagtatt aggattacag
    13801 ttaatgaata tgattttttc ttttgtatgt ggaccgatgg ctgctaaatt tttcaaattt
    13861 gcaaaatctg gtgatcaaaa aggaatcatc aatctgcact tctttatgtg gtttgtccac
    13921 tatgcgcttt atgctcttat tccattcttt gccgttttat ttggagcaga tgttgtcaaa
    13981 agcgtcttgg ataatattcc agaggtcatc atgaacggat taacagtcgc tggaaatctg
    14041 ctgccggcag ttgggatggc gatgctgtta cggatgcttt ggagtaaaaa tattgctgtg
    14101 tggtttttct ttggggccgt tgttatggcg tatctcgagt taccattgat tgcaattgcc
    14161 gcagttggaa cgattattgc aattgttata gcccaaagag atctacaact aaaaaatata
    14221 tctgttcatg cagttcaaag cgatgctgct gtaaatgcaa cgcttgacca agaagaggag
    14281 gatttctttc aatgaaatta gctgatggaa tttctaaaga tgagaaaaaa ttggttcgga
    14341 agatgttttg gcgctcgatg actatgtatg cttcggtgaa tccggtgacg atgggcggcg
    14401 gtggtttcgc ctattctatg atgcccttca ttaatcattt ctacaaaagt gaagaagatc
    14461 gagctgcggc gctggaacgt catacacagt atttttcgac gaccattcct tgcgcgtcct
    14521 ttgtcatggg gattgcggca tcgatggaga aggaaaattc tgaaaaagct ggttttgacg
    14581 tggaaagtat caacgcgatc aaaacgagtt tgatgggacc attggctgga attggcgatt
    14641 cattgttctg gggagtttgg cgtgtcgtta ccgcgggact agcgatcaac ttagccaatg
    14701 ccggaaatat tttggcacca attatctttt tagttatgtt caatatacct aattacttga
    14761 tgcgatacta tggcggcgtt ttaggctatt cattaggctc taaatatatt gaaaagctgt
    14821 atgcgaacgg gttaattgaa attctgacaa aagctgctag tatcttgggt ctgatcatgg
    14881 taggtgcaat gacgatcagt atggtcaaat tcaataccgt tttgagcttt tcaatgaagg
    14941 gcgaagaact aatgaatgtc caagaaatcc tcgatagtat ttttaaagga attgtaccgt
    15001 tattggtaac attgagctgt ttcaaattac tagataaaaa agtcggcgtc atcaaaatta
    15061 tttttggaat ggtcgtcatg ggaattttat tgtcattact tggaatcgca tagtaggaag
    15121 ctgcagctac taggctgcag tttttttagc aaggagggaa aagatgacta ttaatgaagt
    15181 aatttattca cccaaacacc cattttttaa tgctgagtta aacgacggaa atgtgactac
    15241 ttcaaacgaa tacagctact atcggaatat tcctgatgta aagggattga ccttcgatgt
    15301 aagtaatcaa aagacattag caaaccttga tgtttgcggg acgattcaaa cgttgacctt
    15361 ttatcgagaa aatttactaa ctgaggaaaa accaggtgtt tgggtcaata aacagctctc
    15421 tcaaaccaat tcgttatcct taaaggttga agtaaacggg attgtctacc atttgtcaga
    15481 atcggatcat cgtattgaag tcgatgtgat tcaagactgt ttgcctagat atattcatca
    15541 ttattcgacg tttaaggtca ttgtagtatc ctttgctccc atactggcta ataagcgttt
    15601 aagtatgctt gttcaacagg tttacattgt gaatgaaaca cacgaaagcc ttcaagtttc
    15661 agtgaaagat gtgccttcgt atcaagagaa gtattcggat cagcaaaatg tgttgatttc
    15721 tcaaaaagga aataaggaca cgatcgcaca acaggaagtg gcaagcttcg ccattgcttt
    15781 tgtggaccca aatgcttttg aagaagtaat gacttttcag gaatctgata tcgaaaaatg
    15841 gctgaaggaa acattgaact attttgctca gctatttggg cagctgtcaa tgccaaacga
    15901 tagagttgtc catctctatc gtcgtgcact ttatcaaagc ttttctagtt tcggtatgaa
    15961 tcgacaggga caggtcgtcg gcagcaattg gggcagctat cctgctacta atcgaatttg
    16021 gaataaggat atgtattacg catcattgcc attcaccatg tttgaaccgg agttatgtca
    16081 aaaaacggtt ctatggtttg atcaatatgg cgttaagttt cctggttcaa aatttacagg
    16141 aggtatcaat cattctctga gtaattcgtt atccagtgcg ttactggcta gtctttattt
    16201 tgaacatact ggggatacaa aattctttga gcggtgtccg caggttctac tgaatgcttc
    16261 aagaattatt gaggacatat tagctcaacg tcggtcaggg gaaccaatgc tttttccatc
    16321 tgtttggtta tcagacgcct ttgcacttgg aaagtatcat accggcagca atctttgtct
    16381 ttggaaggcc tgtattggac ttgcagaaat ttttgaagcc ctagaacaac tgagtcttag
    16441 caagcgatac aaaaacattg cctgtaaact aaaggaatca atttgtaaac aaatgactat
    16501 cgatggtcct tttggtgagc aatttttgga gggaatcggt gatgaggaga aaacaaccta
    16561 ttcagtgaag cactatcaaa aaccgattct tgagcaaggg ttaatctttt taagtgatgt
    16621 gattgtggat ggaaaaatta atttattgat gcatgatgga gaagaatcag acactacgtt
    16681 gatgcctttt tatcaatttt tagataataa agatcattta tatcagaaca ccatggcctt
    16741 ttctgctagt tctttcaatc caacctatag cgaggaaatc aaaggcatca cttggggatt
    16801 agaatccggc gccacttttc ctggctttat cactgtttta atgagcgagc tgcacaaccc
    16861 tcaagctttc gtaacccgat tagaagaact aatttgtttg gcagatttag acggttcgtg
    16921 gtggtggtgg ccgtatcgct tagaggctca acaaggagaa gtcgtgcgag actttggctg
    16981 cggcaaatgc ggctgggcat cgggtctttt tgtcagccta atgatcagtc aatatttcgg
    17041 attgaaattc aaaaaaggag tattgcaaat tgatcctgtt gataatttaa cgttttcttg
    17101 gaagaatttg aagttcggtc aagccttcat ttcgattgaa tgtacgcaat ccgagttgag
    17161 tatttcaaat ttgtcagaaa agccattgaa aataagtgat acgaaaaaaa ttcttcacgt
    17221 agaaaaggga aaaacgattc acattcaaag gagaaaaaga tgagtatact aaaaaatatt
    17281 acccaattta aaaaaatcca gagtcgagca atcactcccg aaaatccaac aggcgagaaa
    17341 ggtaaagctg cgatgttgcc aagcgagcta ggaccaagcc gcaaaggtcg tggcgcaatt
    17401 ccgctgactt ctggtgaaga aactgtgatt gcggatatta ctggaacggg aataattcgt
    17461 catatgtgga tgacgattcg tgatcgaaca gaatttggca gttttgtttt acgtgattta
    17521 atcttaagaa tttattggga tgggtcggat agccctgctg ttgaaagtcc tttaggggat
    17581 ttcttttgta atgggtttgg agagcgttgc gaggtgaact cgttgccgat agtggttaat
    17641 cctacagggg gctttaattc ctatttcgaa atgcctttcc aaacatccgc taaaattacg
    17701 atcgagaatt tacatccgaa ggatatgtcc tcattttttt atacgataaa ttacgctttg
    17761 gtggacgaat tggaagaaaa tagcctatat tttcacgcaa attggcatcg ggaacgcatt
    17821 accaaattac aggaggatta taagttaatt gatcgattaa agggtcaagg ctattacgtg
    17881 ggtacttacc ttgcgcttac ctgcctagaa cgttattggt ggggcgaagg cgagtttaaa
    17941 ttctacattg atggggatag tgagtatccg accgtaacct caacaggttc agaagattat
    18001 tttggcgggg cgtgggcctt tcatgagtac gatgagttcg gacgtccaca tgcgaaaaac
    18061 tattcaacag cctttttagg gtatccttac caatcaaata gagatgccac aagagatcgt
    18121 tttgaaacgg gaaaattgaa tgccgttcat gcctttggtg acgacgcaat gccacgacat
    18181 ggtctttatc gttggcatct tcaagacccc atctgtttta ccgaagattt atcggtcact
    18241 ttacaacaaa tcggcaacga tgatgttcgc ttatttgagc ggcaagacga tgttgctagt
    18301 gtcgcttact ggtattcaac agaagcttca ggaaatacta aacgtcattt cactgatcga
    18361 gaacgcttgc cgcgttaatg aagtgttttt cacatattcg aagggaggaa cctatgatga
    18421 tatatggggc aattgaagct ggagggacta aatttgtctg tgcagtcggt actgaggaat
    18481 taacgatttt ggaaagagtc agttttccca caacaactcc agttgaaaca atgaagcagg
    18541 tcattgattt ttttaaacct tttaaggaac aattggcagg catcggggtc ggctgctttg
    18601 gtccaattga tattcaatgc aaatcaacga gtttcggtca tatcacatca acaccaaaac
    18661 ttgcttggca aaatttcaat tttgtaggaa cgctaaagca atatttcaat attccgattg
    18721 aatggacaac agatgtcaat gcggcttgct atggtgaata tgttgctggt tctggcaaag
    18781 ggttagcagg tgttgcatac ttcactgtgg gaacaggtat cggaggtggt gccttgttca
    18841 atgggacttt cgtggaaggg ttcagtcatc cggaaatggg gcatatgttg atcaagcgac
    18901 atccaaagga caatttttca ggaaactgtc ccttccacca agattgttta gaggggatgg
    18961 cagcaggacc agcgattgaa aaacggttgg gtgtaaaagg ccagaattta ttggctgacg
    19021 attcgttttg gcagattgaa gccttttacc ttgcccaatg tgcatacaat acaacactga
    19081 tgttttcgcc agatagaatt atttttggcg gtggtgtaat gaaacaagaa cacatgaaga
    19141 aaaaagttca ggataaattt gttgagctta taaatggata tgttgaaata ccaccaattg
    19201 atagctacat tattacgccc gaattaggtg ataatgccgg aataatcggc ggattagcat
    19261 tggccagaaa agcggtaaga aataagcaac cataattaat gaatgtttca ttgatactca
    19321 tgtcaaaagt atttaaccga gtaatcaaat gatcaaatga agttggataa agcgaaaatg
    19381 aattcaaata aaaaggctct aagtcaaatc gtgttgtttt cattaaatca ctgaaaggaa
    19441 atgcttctgc gtttcctttt ttctttttgt tgtgcgctgt ataccttgag gatattcact
    19501 ggtggcgtac acttaaatag taatatattg tgttacttta tgtctgtggt aatagttata
    19561 gttgcttacc aatgtatgtt catttttcga aatgttgact tcataatcct tggcttaatg
    19621 tttgaatttt tttggtgata gattttttca gccgtataaa ataaatcgta attctattat
    19681 agagagttgt gattataaaa agatagcaag aaaaaaaaag aaagtttcta tacattttaa
    19741 atattttatt cctatttcta ttgctaaaaa agtagaaatt ctatatttac ctttaactct
    19801 cgcttctctt gcaaatgtag ttagcatttt aatcgataat aaactcaggt agttatatta
    19861 ccttgaaatg attgataatt ttgtcacttc tatttttttt gcaataaatt ttttatttta
    19921 gtgaaaaata agaaactatt tctttgaagg cgataagtta gaaaaatatt attgtagtct
    19981 tttctttatt aggaatcatc actgttgtat cttattacct tttctgtttt tcttattaga
    20041 gaaaaataat ttgagaggta gatagttttt attggaggaa gtaattatgt atatttttga
    20101 aacaaaaatt tttcattgca ataattgcaa caaagaaatt tcatcggggg aagaggtttg
    20161 gacaaaaatg gaaatttccg ccaaaagtaa ctgctcctca actgaaatca attaaagaac
    20221 tagaatttga gaatgcactg gtaatttgta tgacatgtac tgatgaagta tttcgaaaac
    20281 atttttaaag gttgagatgt atatagattt aaccaatcat ttatctctct cggaaaagga
    20341 atattaacta accagcgggt tatcaaaaat aggtaggaaa actgtactta atggcttatt
    20401 tcgcgctgat tatgagaata tcagtctgtt tgattaattt ttagggttca aaatacagag
    20461 acaaactcca ggtcgggttt cgccacaagc gaattacccg atctggagtt ttgtctattg
    20521 caggaaggcg tagccggact gcaggaataa agcccccggt tctaacaggg ggctaacaaa
    20581 gaacaggaaa ctgtgacaac tacccattgg ggagtgtcct agcagtattt tctttgtcac
    20641 gtgcttatca attttgtcac ctcattttga tgatggatac gctgcattta acaggcgttc
    20701 tttctcgact tccatcacga atggtagata aagatattgc tgttctagta gatattgatt
    20761 gtcctctgtg tactgataag aagcggtttc ttctgaatag accaacgggg caattcgcac
    20821 atcgctggtc aattgattaa ttgagtatag aaggatggct ttctcaccta gttccttggg
    20881 taaatgtttc ccaaaatagg aataggattt ttgatttgct aaatagacga gtatgcgatc
    20941 cacactaaag ttggagaagg taaagggcat catgtgcaga atatgatttt gattatcatg
    21001 ttgcaaatag agctgggctt ccttaccaaa attagttgaa ccaagataaa gctgcttttt
    21061 ttcatcgata cattggaaag taaactggtc ttgttcagtt ggtggaatga gatttgtaat
    21121 aaaaaattca atcattcgat ccatttcttt ttcactaaca aggtcagcgt gtttgatttc
    21181 gtctactatg agaccttcaa tcttaggatc aagcttacct gccttttttt ggagcatttc
    21241 taaaatgtat tgattctctt gatcaccata caaaagatat tcattggtga catgacctat
    21301 ctctgcgatt tggtgaaggg tttcggtttt tggaaggttg atcccacgtt cccagttatt
    21361 gactgtactt cgcggtaaat caccaattag tttgccaaat ttctccatac tcaactttaa
    21421 ttcttggcga atctgccgaa ttcgattgcc gactgcttgc ttgtctatct ccatgaaaac
    21481 tcctcctcag gataagttct taaatttagt atacgtgtgt acttaaaaaa agtcaaaaca
    21541 tattgactta aaataaatac aggcgtacaa taaaaatgta cttaaaataa gttcaatagg
    21601 ttctttgaca agtaaatagc agaaggaggt ttgtcgatga accaagttaa tcaacatgat
    21661 ctcggtgaag ccattcgagt ttctcgagaa gaaagggggt ggacacaacg atatttagca
    21721 gaaaaagtgg ggatttcgag atagttatta tcgaaagtag aaaaaggaac gagacaatta
    21781 agtgaagaaa aattgaatct tgtgttggat agtttgcaag aagaggtcgt tccagttaac
    21841 cgtgttttga ttgattatct aaccattcac tttttctcga atcaacattt aaagctaatt
    21901 gaagagatta ttgaaatgcc cattgaacgt tttgaggaac tggattatgc gccaaagggc
    21961 tatattggcc aatacgtgtg gaatcaagta atcacgattc ggtattccat tgacgatgcg
    22021 gttaaaggaa ctgtgatgga gttttcaggt caaggctgta aacacttagc aagatggcta
    22081 aaaacacgaa aaacaaattg gcaacagttt tttcgtaagg tattagatta tcaggggaac
    22141 tttacacgga ttgattttac tttagatgat tttgtgggga gtctcagcat tccagaactt
    22201 aagcataagg taatactagg ccatgtgtgg accacttttc aagtgagtga atctcatggc
    22261 ggtacggata tcataaacaa tgaatcaaat ggtgagacct tgtatttagg ctcaaagaag
    22321 agtcagtgtc gtttttgctt ttatcagaag gaccatgaac aacggaagcg acgaggaatt
    22381 cctttagaag aggcaaaagt aaagaatcgc tttgaactac ggtatcgaaa agagaaagcg
    22441 caaagcttgg caaagattat ttcaaaaacc catgatttaa ccaaactgtt ctttgagtta
    22501 cttaatggag caatttgttt ttatgaccgt gatccaaatg atctaggtgc aaaagttgat
    22561 gccaaatggg cagcctttat tagcaattat ggcgcaatca ccatttcttt agatacaatt
    22621 cctcaaagtt ttgagaaaac tatgagttgg ttaattcatg gtgtttcacc atcattagtt
    22681 tttattgcac aggtcgatca aacgtttcat tcttccttgt tgcctttgat tattgaacaa
    22741 ggagaaatga atccaagaca gatcaaactt ctccaaaata tgaaggcaga cccggagtat
    22801 tatcaggagg aggttgagta ttatgaacga aaattacaaa gtatatcaaa tgagaaaaca
    22861 agctactaaa aaagcaaagt actgcttaca caacaagttg tgcaacgtac tccgcctcga
    22921 caagacgagt ataacacaac catcgaatga gaaacaatag attataaaga ggattatttt
    22981 aaaaggagta aagaatcagg aggtgagaga atggaaacaa aatgtgattt catggtaaat
    23041 cgtgcaattt tgatagagat gggctttaaa cctagtcaag ctgcacgaat gattaaagag
    23101 agcaaggcat atcttgcaag gattgaaggt attgattttt ataataatcg ccaagttggt
    23161 gtggtgccat cccgagtaat tgaacatctt tttcacattc aagttgcgga ataaacagta
    23221 caagcgcggt actattaaag tagcttttct caagtacagg gaggaatgga acgatgccaa
    23281 aaattaaaaa tatttaccaa gataaaaaga caaaaaaata gttcttccga gcgtatctag
    23341 gtatagatga aaatggaaga aaggttcaga agactaaacg aggttatgct tcacaaaaag
    23401 atgccaaaat tgcttatgac aagtacatgg aaacacatga ttttaacaag acaattccta
    23461 atcaactgtg ttctaccaat caaatgactt tcgaagagtt ttacaaggat aggtttgtaa
    23521 aatggtacga gagacaggtc aaaagtcaaa catatgagaa tgcgcaattc attttcgaaa
    23581 agcgtatgca gtttttctat cattttcgtg ttcgagatat tacaagtcac gatattgagg
    23641 attggttatt tgaactaagc cagacagagt cacgtaattc acggaaaaaa gttaatggcg
    23701 aaacgttatc aaaatcttat atcaatcgga ttcgagggca cctaaaaatt gtattggatc
    23761 gggctgttaa agagggggtg attgctaaaa atcccgttga agatgtttct tcgcttcctt
    23821 ctgagaataa gaaagttgag ttttgggaac acagtgagtt tcaaaaagtg atgaatgaat
    23881 tgaaggatga taagattcaa acccaccatc ggaagattgt gtatgaaatg ctcttttata
    23941 ctggcattcg gattggagaa cttgaagctt tgtcttggtc gaatgtagat tttgagaaaa
    24001 acacgataac gattgagaag acattgattt ataatacaaa agatgattgg tacttttcga
    24061 ctcccaaaac taaatgtgct tatcgaacga ttgggttggg gaagaagctt tccttaaaac
    24121 tcagacagtg gtatgacttg caaaaacgta ttggaaattt tgaatatgtt gctcagttgg
    24181 acggaacatt tacgccacct tattcttttg cgaactggtt aaaagaagct gctaaaaaag
    24241 cgggtgtcaa accgattaag cttcacgcgc tacgacattc tcatgttgca tttctaattg
    24301 agcagaatat tcaaccgcta tctattcaag aacgattggg ccatgcgaat attcaaatca
    24361 ctttaggaac ttacggtcac ttatatgcaa agtcagatca acaagtggta agtgcgattg
    24421 ataatgttca agagggcgct attctttcaa aaaatgacaa tttattacca gtctgagttt
    24481 tccacggttc taccataaga caaataaatg cttttgcacc agtgttttca gtcgaattgc
    24541 tcctgttcaa gatgtatcgg aaatagcgag aaaataagca aatttgcata actataa
//
LOCUS       DBNICI010000027        24372 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.027, whole genome shotgun sequence.
ACCESSION   DBNICI010000027 DBNICI010000000
VERSION     DBNICI010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.027"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="AC1ZCC_000881"
     CDS             complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="AC1ZCC_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HIQ1629152.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1551..2948)
                     /gene="tyrP"
                     /locus_tag="AC1ZCC_000882"
     CDS             complement(1551..2948)
                     /gene="tyrP"
                     /locus_tag="AC1ZCC_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HIQ1629153.1"
                     /translation="MGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIFAVLFFAGP
                     ISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVASTLGPLLG
                     NTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYIPVVIMFVL
                     GVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMSSVYMPRLK
                     DATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDCQILGLPEV
                     IGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYGVSRNVVLT
                     QSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKKHPEFERPY
                     RIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVIPLIINHFK
                     KDSWGIEVEKSLEEK"
     gene            complement(3215..5092)
                     /gene="tdc"
                     /locus_tag="AC1ZCC_000883"
     CDS             complement(3215..5092)
                     /gene="tdc"
                     /locus_tag="AC1ZCC_000883"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HIQ1629154.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5383..6639)
                     /gene="tyrS"
                     /locus_tag="AC1ZCC_000884"
     CDS             complement(5383..6639)
                     /gene="tyrS"
                     /locus_tag="AC1ZCC_000884"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIQ1629155.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(6751..6956)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7219..8520
                     /locus_tag="AC1ZCC_000885"
     CDS             7219..8520
                     /locus_tag="AC1ZCC_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIQ1629156.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8589..9188)
                     /locus_tag="AC1ZCC_000886"
     CDS             complement(8589..9188)
                     /locus_tag="AC1ZCC_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIQ1629157.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9442..10323
                     /locus_tag="AC1ZCC_000887"
     CDS             9442..10323
                     /locus_tag="AC1ZCC_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIQ1629158.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10336..11946
                     /locus_tag="AC1ZCC_000888"
     CDS             10336..11946
                     /locus_tag="AC1ZCC_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1629159.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(12023..12682)
                     /locus_tag="AC1ZCC_000889"
     CDS             complement(12023..12682)
                     /locus_tag="AC1ZCC_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIQ1629160.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(12965..13630)
                     /locus_tag="AC1ZCC_000890"
     CDS             complement(12965..13630)
                     /locus_tag="AC1ZCC_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HIQ1629161.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(13777..14382)
                     /gene="dhaL"
                     /locus_tag="AC1ZCC_000891"
     CDS             complement(13777..14382)
                     /gene="dhaL"
                     /locus_tag="AC1ZCC_000891"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HIQ1629162.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(14410..15420)
                     /gene="dhaK"
                     /locus_tag="AC1ZCC_000892"
     CDS             complement(14410..15420)
                     /gene="dhaK"
                     /locus_tag="AC1ZCC_000892"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HIQ1629163.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(15395..15790)
                     /gene="dhaM"
                     /locus_tag="AC1ZCC_000893"
     CDS             complement(15395..15790)
                     /gene="dhaM"
                     /locus_tag="AC1ZCC_000893"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HIQ1629164.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            15944..16264
                     /locus_tag="AC1ZCC_000894"
     CDS             15944..16264
                     /locus_tag="AC1ZCC_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HIQ1629165.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(16376..17233)
                     /locus_tag="AC1ZCC_000895"
     CDS             complement(16376..17233)
                     /locus_tag="AC1ZCC_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIQ1629166.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(17341..18645)
                     /locus_tag="AC1ZCC_000896"
     CDS             complement(17341..18645)
                     /locus_tag="AC1ZCC_000896"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIQ1629167.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(18642..19151)
                     /locus_tag="AC1ZCC_000897"
     CDS             complement(18642..19151)
                     /locus_tag="AC1ZCC_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629168.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(19326..21461)
                     /locus_tag="AC1ZCC_000898"
     CDS             complement(19326..21461)
                     /locus_tag="AC1ZCC_000898"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HelD family protein"
                     /protein_id="HIQ1629169.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(21544..23199)
                     /locus_tag="AC1ZCC_000899"
     CDS             complement(21544..23199)
                     /locus_tag="AC1ZCC_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIQ1629170.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(23201..23515)
                     /locus_tag="AC1ZCC_000900"
     CDS             complement(23201..23515)
                     /locus_tag="AC1ZCC_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIQ1629171.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            23685..23945
                     /locus_tag="AC1ZCC_000901"
     CDS             23685..23945
                     /locus_tag="AC1ZCC_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HIQ1629172.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            23959..24273
                     /locus_tag="AC1ZCC_000902"
     CDS             23959..24273
                     /locus_tag="AC1ZCC_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIQ1629173.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT         6945 a         5036 c         4397 g         7994 t
ORIGIN      
        1 aaaagcaatt ttcttgagtt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
       61 cttttttcct tccttcaatt ttttacacgt cgacgattca ataaaaaacc gcctaatatt
      121 gtaaagacag gtgcaagaat cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact
      181 ttcatcgttc ccataatgaa tgtgccgctg actccccacg gtacgagaga attgacggtc
      241 gcacctgaat ccgctagtgt tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa
      301 gaacgcttga atgtttctcc tggaagaatg atggacagat actgttctcc aacgatcaga
      361 ttgattccga tacagctcaa ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt
      421 tcttttaatt cttgaacgat ggtatcaacg atcagatatt tgatcagtaa cccacctaat
      481 gccaatgcaa gtaaaatcaa actagcagaa cctaacatac tcatgatccc gcctcgagaa
      541 agcagttcat ctagctttgg atcaggtgta tttgcgacat aaccattcat caagatgtct
      601 gctgcttttc cgaccgtcaa acttttctca tggataaaag aaagaacgag agcagctgtc
      661 gatcccgcta gtaatgtagg gatagccgga atttttttga ttgccccccc aaataaaata
      721 actagtggaa tcaatgtcca aggagagatc caaaagctcg ctttcatgtt ttggatcatt
      781 tcattgatac tagctagatc ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc
      841 aacgaaagga cgaatgctgg gaaatccgta taacgcatac cagcgatatg ttcgaataga
      901 ttaactcctc caatacccgc tgccaagttg gtcgtatctg ataaaggcga gcagttattc
      961 cctaaaaagg caccggaaac gactgcgcct gtagtcattg catcattgaa tccaagtaaa
     1021 tgaccgatcc ccaaaaaagc aattccaatc gtcgaaatcg ttgtaaatga actgccgaca
     1081 gtcactccga caattgcaca aacaagaaat gctgtcggta aaaagaaacg cacagaaaca
     1141 agtttaaatc cataaaccat aatcgtaggg atggttccag ataaaatcca tgaagcaact
     1201 aagacaccaa tcaaaagaaa aatcaggatt ggaatgatcc ctggagtgat tcctttgatg
     1261 atcccgtcat ggatctcatc ccatgtgaac tttttaaatc tgccataaaa caacaaacac
     1321 atgaaagctg caaggatcgg gatatgtggc gagagctgga atccgatgat cagtactcct
     1381 aggagtgcca acaacacgat caatacagcg atactttctt ttaaagaaat atttttattc
     1441 atcgtcgatc tcctatttca aatgaaaagg ccctgccatg aaaaaactat tcacttggca
     1501 ggacctccta ctaaatcagc gacaaaacgt cggctgaaag acttattttt ttatttttct
     1561 tctaaacttt tttctacctc aatcccccaa gaatctttct tgaagtgatt gatgatcaat
     1621 ggaataacga acatgaccac tgtgatagct gctactaata atgcatcact caatgtcgct
     1681 gtacctaatg tagcaaatac gacaacgatg atagagaaga tcagcatgca gctgactacc
     1741 caagctaatc cgtttccgtt tttaccaatt cggtaaggac gttcgaattc tggatgtttc
     1801 ttacgcattt taagcaatga aacagcaatc aaaatgtaca cgatacaata aataacagtt
     1861 gtagcatttg taagtgtcaa gaatacagca cttacgtcat ccattacacc ataaagcaaa
     1921 gcaaataatg aaataacgat tgattgtgtt agaacgacat ttctagatac gccgtatttg
     1981 ttttctttat ggaatccgaa ttttggtgga aggaatcctt cacgagctac ttggatgatt
     2041 gtttttgatg gtccagtaac ccaagctgat aattgtaaaa gcacaccaat aaataccatg
     2101 aagctgaaga tatttccgat cacttctggt aagcctagga tttgacagtc aatcaagata
     2161 ggctgcgtga tatttgctaa ttccattttt ccatctggta cgacatttgc tacaagcatc
     2221 gcattgatga cattcaataa aactaatccg atcaaagcga taaatacgcc ttttgtataa
     2281 ttttttgttg cgtctttcaa acgaggcatg taaacagaac tcatttcgat cccaacaaaa
     2341 ataaatgtga taccagctaa gtatttcaat gaatcaatat gttccaagtc agggaaagct
     2401 tttgaccatg aaaagtcacc tagatagccg tttgagacta gtccgacttt gaaggctgcc
     2461 aaaacaccta aaacgaacat gatgactact gggatatata cccctagcca tacaccgatg
     2521 ttcccgccga cttttgccat atcgaatttc aagttcaaga tcgtgatgat ccagtagata
     2581 actaaaatac aacctaaaac gaaccagtga ttattgccaa gttctacatt accaaatgtg
     2641 tttcctaaca atggtcctaa agtagaagcg accattacca tacctgggaa catttgcacc
     2701 cataatagcc atgcaacgac aaatccccat ttgctgccca gtgctgtttt tacccataac
     2761 tgaggaccgc cttcttgagg aagcatcgtg gaaagctcac cggaaaccat tgatatcgga
     2821 ccggcaaaaa acaaaactgc aaaaattgaa taaaaaatca atgtccaacc gactgcggct
     2881 aacgtaggaa tactccgaac cgttgcacac agtgccattg tcagaccgat aaaggtccca
     2941 agacccattt tacttacttc agatttactc accgttgaca ctctccttga ttttttggat
     3001 aaaataacgg tgtctttgaa gtagtttcag caataaacga aacaattcag cgaatatttg
     3061 aggggattct ttgagtttat tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg
     3121 gatcgaagag cttaggtctc cccttgctct tcagatccaa acatgcgtca gaaacagtta
     3181 gtattcaagc tgttttctaa catgattacc ttgcttattt tgcttcgctt gccaataatt
     3241 ggtctgcgta gattttttcc aatttttctt gaagagctgc tttgattttt tcagcatatt
     3301 cttcaaagtg ttcttctttg ttcatgtatg gtgtcattac gctggcacgc aatacagtta
     3361 cttttccggc acggttccat tcttcatctg agaatcctag ttggttcaca aattgtaatg
     3421 ggctgttgcc atagtctggg atagcaaagt cagtatgtga agttaagaac tcattaccgt
     3481 aaatgcttcc tttcacgtat gaagaataat catatacgtc atggttcaat ttattcattg
     3541 caactaagtc atcgttgcct ttttctttga atacatagtc taccatattg aaatctggat
     3601 aagttaatgg atgaacttcg atttctttgt ctcctacttt aaatgacaag tcattcaaga
     3661 agttgtagaa acgatgtgca ccttcgattg atgcacccat taatttacca tatcctgtga
     3721 cattcaaagg taagacatgg tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg
     3781 aaccttccaa gatataagcc ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat
     3841 atgtcgcaaa gtaagagata acgtctctca tgcggatatc tttgatgacg ataccaccgg
     3901 cagaatatgg aacatagccc attttatgtg ggtcaatcgt tacagattct gcttcttcga
     3961 ttgctttata tgcactgtgt acttcttcta agatataatc tttgttttct gtaaagacat
     4021 tgtatttgta atgaacatct tttaaatctt caaatggaat gaagttgttg tcttcgtcta
     4081 agaaaatcgc acggccgtat ccgccataag cagcatctac gtgtaagtag aagtaaatac
     4141 cgtctttttc taatacgcga cgcaatgcaa cgattttatc gataccgtcg attgctcctt
     4201 cttctgttga tcctacaaca ccgactacac ctaggattgg tgttttttca gctgctagac
     4261 cacgaacgat tttttcaagt tcgttgatgt ccatgcgata gttgtggtca actggcactg
     4321 gaatcacttg gtctaaaccg ataccgatga tgtcggcagc ttttagccat gaatagtgtt
     4381 tagtttgtgg aaccaaccat tttcctaatt tttctaaatg ttttccgcta cgtgctgagt
     4441 gagctttgat ttcgtcgatt ttttctggga cgctgtctaa cagattcatg atttcttctg
     4501 ttgacaagtt cattaattcc caatcgcttt ttccagcaac tagttcaggt gtcacttctt
     4561 tcattgcaag cggcaatgat ttgatgttgc gggcatacca aagtccttct aagtttgcta
     4621 aagaaccatc agcaacgatg tggccccagc catctttata gctcattaat tttgcaaatt
     4681 ccattcctac ttcttcttcc atttggcttg ttgcaggaga agattcatag gcaacgttat
     4741 taccattcca tagcatagca aaattgtacg ctagtaaaga tggcatcaat gtttcagagt
     4801 tcatatgacc ccagtaacgt ccggcattat gccaaggaac ggaatgggta cgcatccgcg
     4861 ctgaaatttc tgatagaaca tcctttgttt tattcacagt gtgttcaaaa ctagcactgc
     4921 ttttttcttc tggcgtaata ataggcatgt cttgaggcat gtagttttga cgccagccta
     4981 aatgttcatc caccaattca ttcaacaaag ctttatagat ttggccgttc tcagctttgt
     5041 ccccaatgaa tagggcattt aggtttagat ctttcgacaa tgattcactc atagtgaaac
     5101 cctcctaaaa aaatatttaa attttgtcga gccctacatt acacctgttc ttctattctg
     5161 taaactaagt tttggcctga tttttaatca tattttaata tgtatcaatt atccgaattg
     5221 ttcacacttt tgaaataatt aaaaaaaacg agggaattta caaatgtaaa ctccctcgca
     5281 aagattgtat taacgacaac cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt
     5341 gtaatagtat ttcccttttt caaggtaaaa acttactaaa ttctaatcaa ttactttggc
     5401 caaaaagtaa tttttcttac cttttcgaat aaccacaaac ttaccatcga attcatcgga
     5461 tggatttatg acaaaattta aatcggtaac gcggtcaccg ttaatactta tagctccgtt
     5521 tgaaacatct tcacgtgctt gacgtttaga tggttcaatt tttgtggaaa caagcagttc
     5581 cacgatattt tcaggtgtgc ttgaaatttc aacgttcggc attttaccga atccttgcgc
     5641 gatttcttcc gcatttagtt ctttaatatt tccagagaac aatgcttgcg ttattttttg
     5701 cgcttctttc aagtcttctt cactatgcac aaatcttgta acttcttctg ccaaacggcg
     5761 ttgtgcttca cgtttctcag gttctgtctc tacttttttc gctaagtctt cgatttcttc
     5821 ttgagacaag aaagtaaaga atttcaagta tttcactaca tcgcgatcat cttgattcaa
     5881 ccagaattgg tagaactcaa atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc
     5941 tgcagttttt ccgaatttcg ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa
     6001 cgcttctgct tctggtcctt ctaattttcg gattaggtct aatccagctg tgatatttcc
     6061 ccattggtct gctccaccga tttgcaattg cacatcgtat gttttatgca gatgcaaaaa
     6121 gtcgatagat tgcaggattt ggtaagtaaa ctctgtaaat gagattccta cttccaaacg
     6181 gctggcaacg atatcttttg ctaacatcgt gttgatgttg aagtttttac cgtagtctct
     6241 caaaaattca agtaaagaga tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt
     6301 tgcatcttta ccaaacaatt ttctcatttg attggaaagt gcgtccacgt tatgttgcac
     6361 ttgttccatc gtttgcaaca cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc
     6421 tgttccgccg ccaatcagaa tatatggatg atggcctgcc aattggaatc ttttcatcat
     6481 cataaatgga atcaaatgtc cgatatgcat cgaatcacca gttggatcaa cgccgcaata
     6541 aagtgaaatg ctcttttctt caacgagctc gcgaagtctt tcttcgtttg tttgctgatt
     6601 aatggcgtcg cgccaagtta attcatcaat aatattcatt ttactgacct ccgtgtagtt
     6661 tttttatgaa agcaggaacc tgctagcata tgtaattaat tatttcttca ggataggata
     6721 ggaaataaaa aaagtccctt ggcatataag ccaagggacg aaatctcgcg gtaccaccca
     6781 atttgtatgt ctagatatcc aacatacctc tcgaacattt aacgctgtcg cgtggcaatc
     6841 actgccaaag actcctgaaa tgtaattcgc tttaaagcat gtactggttt gcagcgacca
     6901 ccagctttct gaaacattgg ctttaacaac tacttcgttt cgatcttcgt cttgttatta
     6961 ttaatatgtg tctatcatag agcgcaactt tttctttgtc aacagaaaaa ggatattttt
     7021 tttaaattcc tcattttttc ttttttgaat ccgctctaat tccgaacatt ttattttcaa
     7081 ttattgttaa aagaaataac tttttctgta ttttttctgc aatttatcga gaagtcactt
     7141 tactttgcct cttattcttt cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa
     7201 aagcaaggga cgaggtatat ggaaaaaaag caaaaaatta ttttaggagc cacttctgct
     7261 ttaactatcg ttattttaat tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa
     7321 gaaccaatca ttcctgaaaa aaaaataaca gaaaaagcaa aagaaccgac agatgataag
     7381 caacaactag ttactcaagc aaaaaatctg gctcaaagct acgattatga ccaagcgatt
     7441 gcgttattgg aaaaagatag taagaacgga tctactgaaa cacaacagct tcttcaaaca
     7501 ctaaaaaagg agaaagaaca actgatcgac tggtctgatc ctactcagat ctctcatgtc
     7561 tttttccaca gcttgatcgt ggacccgcaa aaagccttcc actctcaaca ggcacaagga
     7621 tacaaagatt atatggtgac tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa
     7681 aatgattttg ttttagttaa tttgaatgat ttgatccaaa aagacgaaca aggaaatctt
     7741 tcctttaacg ggcttgcgct acctgaagga aaaaaaccat tggttctttc acaagatgat
     7801 gtcagctatt atgaatatat ggataattcc ggttttccta gccgattggt cgtatcaaat
     7861 aacaatgaaa tcaaaaatgt ctataaagag aaagataaag aagaaatcgg tgactttgac
     7921 atggttcctc tgatcgatgc ctttgtcaag aaacatcctg atttttctta tcgaggcgca
     7981 aaaggtacct tggccctgac aggttacaac ggagttttag gttatcgaac atcaaaatct
     8041 caatatggag acaatgaaaa aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa
     8101 cagttgaaga aagacggctg gagctttgct tcccatacat ggggtcactt gaatatgact
     8161 caagcttcac tagaagatgt aaaaaaagac aatgaattgt ggcagcaaga agttgcacct
     8221 atcctaggaa aaacaaatat tctgatttat ccatttggtg cagacatcag cgactggcag
     8281 ccttattcag cagaaaatca aaaatttgct tacttgaaga gtcaaggttt cgatattttc
     8341 tgtaatgtcg atgcttctac accagcttgg ggacaattag gtacggattt ctatcgaaat
     8401 gctcggatca atatcgatgg cattcgtttt caatctgatt tagacggaaa aaatcctatt
     8461 cttgatccat tcatcaatgt ccatgatgtc tatgacaaag aagcacgcga atctgcttga
     8521 ttttacgtca tttatgaacc aaaaaagccg aactgacatt ttatcgtcag tttcggcttt
     8581 tttatgcttt atttcttctt cggtgcgatt cccttgatca ggacttctgt ccattctgct
     8641 gtatatggaa gcagttttgc gtatatctcc ggcttagaaa ttccttctac cattgcttga
     8701 aaatagagga tcaagaactc atccgaatat ttcaaatcca cttttccttc tttccgccct
     8761 cgatggaaaa gatcaagcat gatactgaaa ctttcttgcg tatacgcatc catcgcgtca
     8821 gaaaattctt ttttttcttt ttttgtatag tatgtcatca aatcgatata aaattgtttg
     8881 ttcactcgat tgagataacg gattttgttt tcactcatgg caatcagtgt ttcttcaaat
     8941 ggaagatttt ccgcaatcaa cgctttcgct tcttgcccta aatcattcat aaatgcaaaa
     9001 aatacttcat aaatcagccc ttccttgctg ccaaaatatt taaaaatcgt cgctttactt
     9061 acctgagcgt tctcagctac cttatccatt gtcactccag cggttcctat gtctgtgttc
     9121 atcaagtcaa aagctgcttg taaaatctct ttctttttct cttgtgctct cttttcaaaa
     9181 ccgttcattt gtagagcctc ccgtaatttt aaacttttat aatatatata gttcaaattt
     9241 attctgaggt ttattataac tatctattga gaaagtatca agctagcaat tccttgttta
     9301 tgaattcatt gacagaaatt tataaaaaga atattatgta ctaaaataat aaaaaaagtt
     9361 ttataaaaag gtataaaacc tttttattaa actatttaat cttgtttagt atatattttt
     9421 agaaaaagga gtggggtttt tatggcagaa atcgtcaaag tcacacattt gaaaaagagc
     9481 tttggtcgtt tcgaagcttt aaaagatgtc tctttcaccg tacattcagg tgaagtcgtt
     9541 ggatttatcg gaccaaatgg ttctggaaaa tccacgacga ttcggacact tttagggatt
     9601 ataaaaaaag acggcggaac ggcaacgatt tttgggcaag atgtatggga agatagttta
     9661 tctatccatg aacgtctttc ttatgttcca ggcgatatct ctttgtgggg aaatttgact
     9721 ggaggagcga tcatcgatct cttcatgaag cttcatggaa aagggagtgc tcaaaaaaaa
     9781 gatgaactga ttcaacgttt ctctcttgat cctaagaaaa aagcaaaaaa ctattcaaaa
     9841 ggaaatcgcc aaaaagtggg cttgattgct gctttggctg ttgattcgga tctctattta
     9901 tttgatgagc ctacttctgg tttagatcct ttgatggaaa cgattttcca agaagaagtt
     9961 gaaaaattga aagaacaggg caaatccatc ctactttctt ctcatattct cagtgaagtc
    10021 gagcgactag cggataaagt cgtgatcata cgcgaaggaa cactcgatga agaaggaaca
    10081 ctcgatgaat tgcgtcattt gactcgttcg accatcacta tagaaacaga agcagagaat
    10141 agtcacttag cacaattgaa cggtgtccat gactttgtgc aaaaaggtca cctcgctcaa
    10201 tttgctgctg ataacgaagc tttcgatgtg attttagctg aagcgtccaa attaggtgtg
    10261 aaaaaatttg aatctattcc accgactctt gaggatttat tcatgagaca ttatgaagga
    10321 tgaggaggaa gcgaaatgac tgataaattt tcacgctggt ccaccctttt ttctcaaaac
    10381 cttagccgtg actggaaaaa gatcctgata tggctagtcg gtgtcactgc tttttctggt
    10441 ggttttgtcc ctgcgtttga agaattgact tccggacaag gattagctgg attatacgaa
    10501 accttgcaaa acccggcaat gattgcaatg gtcggaccaa ctccagcttc gaatgctgct
    10561 gagtatactt taggagcgat gtatgcaaat gaaatgttgc ttttttgcag tttgtttgct
    10621 gctgtgatct ctgttttaca cgtggtgagc caaacgagaa aaaaagaaga acttggtctg
    10681 attgaactta ttcgttcctt tcgagtcgga cgtcaagcaa actctttagc tgtcatcgct
    10741 gaaagtattc tgatcaattt gcttttagca atatcgatca gctgtacgat gatttttttc
    10801 caagcggatt cgatcactgc agaaggtgct tggctttttg ggctatctgt aggtagtatg
    10861 gggattttgg ggagtgccgt tgcactgttt gctgcacaac tgatgccaag ttcagccagt
    10921 tcgaatggtc tgtctttagc gtttttaggt ggactttacc ttctacgagc aataacagat
    10981 gtagctgatt cctccctttc gatgtggaat cctttaggct ggtcttattt gacctatcca
    11041 tttacaacta acaactggat tcctttgctt tatttgtttt tgttcagtct aatcttgata
    11101 atcagttcat ttgttttaga ggaacatcga gatttgaaca ccggctatat ccccgaacgt
    11161 gaaggaaaac cgtatgctaa acgttctctt ttatccttgc gtggtttatt attctatcta
    11221 aataaaggga tgacaatcag ttggcttatc acttttggtt tgatgggggc ggcatacggc
    11281 tctatctacg ggaatatgca acagttttta gaaagtaacg acttaatgaa acagatgttt
    11341 atccaatctg gcgtctcaat tgaaaaaaac tttacaagca cgatcatggt agtcttgatt
    11401 tgtctagctg ctattttacc aattgctagc atcaatcgtt tatttacaga agaaacccac
    11461 ggacgtatga atcagctgtt cgcaacaaaa atttctagga accaacttta ttggaccaat
    11521 atgttactag ctattctatt tgctatattg gcaatcctga ttagttctgg tgcactaggg
    11581 ctttctgctt tggcttcgat gaaggatgcg aaagacatgg atctattgat gtttcttaaa
    11641 gccgggttca atttcctgcc tgccgtttta gtgtttatcg gaattagtgg ctttctattc
    11701 ggatggcttc ctcgaatcgg taaacttgct tatatctact tagcttactc ctttgcactc
    11761 aattactttg gcggaatttt ggatttacca gaatggtttt ccaaaacagc tgtccctagc
    11821 tgggttccac ggatgccaaa cgattctttt gatctttccg tgttccttac gttatcatgt
    11881 atcagccttg cactgttcgt ccttggttct attggttata ggcgaagaga cttactagaa
    11941 ggataataaa aaagcatgta caatttcatg ctagcctcca aatctgggtc aaaaatccaa
    12001 catttggagg tcattttttt atttacaagt cttctaactg ctctttatca atgatccaga
    12061 cttctcgatt ctttcttttt acataaccat tctcctctaa taatttaaat tttctagata
    12121 aagtttcggg ggtcgtaccg ataaatgcag caagttcttt cattttcatt ggcaggaaaa
    12181 agtgatcctt ccctgtcgct ttagctaagt caagcagata gacagcgact cgttcttcta
    12241 ctttttccat catcaaaaaa gttgtctgct gctgtacttt tgttaatttc tcaacgttga
    12301 ttgctaaaag tttcagacta agggcgggat aatgcagtaa gagttccgag aaatcttttt
    12361 gtctcaatat gcaaatttcc gttttttcta gtgcttcgcc aaataaagac tcatttggaa
    12421 cgcctagcaa ctggctctct ccctcatatc cgcccggctc tattactcgt aaaagctgtt
    12481 cctttccgct agcagataat tgatacactt tcatactgcc ggaggccacg atcactaatt
    12541 gaggttctcc attcggcgca agaatctgtt ctcctttttc cacatagtga tgtttgacaa
    12601 gtgcgtgtac tttttcttga tcttgctgta ctaagtgatt gaacaaagga acaaatgaaa
    12661 cacataaatg atcatgactc ataatctctc ctccttctaa acagctattt ctaataaaga
    12721 aacataactt cccttattcc aactgataat tttcttttat catagcataa acgtcaagcg
    12781 aaccagatac cagcaaatag agtggatttc tttttgtccc ttgctttatt ttccttcttt
    12841 tgagacctta agtaattgat tcctatcaat tttttaaata tttacttttt gaataatcaa
    12901 aaacaaacct tcactcgcaa gccgagagta aggtttgttt ttcgcattgc ttatttcgtg
    12961 ttgtctattg aaatttttcc gcacctagct tttgatatag tggtgctagt tcttgtaagg
    13021 tttcagcggt tatttgctca acgtgttcga tttcttgcgg atagaagaat agtttaccca
    13081 tttgaaaggt tcggtctctc atcgcttttt tcaactcttc ttgtttcttt gtatcggaca
    13141 aatagtcaga caaagctaag tggtcgtcaa gttcatgttc tggttgcggc catatgtaat
    13201 aatccttgat atcgactgtt ttgctccact ctttcagtat cgcaagcaac tggttatacg
    13261 ctgcaatgct accatatcgt tcttcgcttt tataatgttg gtacatcaaa tagatttggt
    13321 attgtttttg attcaacaaa actgctagac aggcattctc gtcttgacga gattcgcttc
    13381 ggtacgcgct ccaaaaatga ctgcgtaaat tccagccatt cgtccagctt tcgattttag
    13441 gtttgctcat gccgtacata tcaggcaaaa gtgcagctgt ctggagctga atctctttcc
    13501 atttttgcca gtgttcttta tattctttct tgatttgttc aatcttatct gttggatggc
    13561 tctctttgag ctgtttgaat gcgtagattg gtcgatcaaa caactgataa tctgattttc
    13621 caagttccat tgtttcgcct ctttccgtta tcttttgtat tctacctgaa ttttgcgata
    13681 tggacaaata gaggttgtga aaaagctgtt ctgcatcaag tcataagaac agccaaacaa
    13741 aaaactgcga tgagcatcac cgcagtttta ttgaacttaa ggttgattga tcacatctaa
    13801 aagcgttgaa aataaaatgg cactagaagc agctcctggg tcgatatgtc cgattgcacg
    13861 ctctcctaga taggaagctc gtcctttttt agctaccaag actttggtgt gttcttttgc
    13921 ctggtcaatg atttcttttg ttaaattccc tgccttcaat gcctctgtga ctggcagcca
    13981 aacatctaac atcgttttat cgtcagcttc tgcctttcct cggctttgaa tacccgctgt
    14041 tccctgctcg atcacttcgc ctaatttttc ttgagaatca atagaatcga cctcttttgt
    14101 cgctttcatc atgttcatga atgcagatcc gtataacgga ccagaagccc caccgacttt
    14161 cgatatcatc gccatagaga atactttgaa tgtatcgctg attgtttcag gatgatcggc
    14221 ttgaaaagct tcttcataag ctgaaacccc tcttcccatg ttatttccat ggtctccatc
    14281 gccaatcggg gtatctagat cactgagata ttgtttgttc tgattgattg cctctgcaaa
    14341 ttgcgtgagc cattcttgga tttcttgaac ggttagtttc atattatcac ccttttatct
    14401 tgagattttt taccatgaaa tcgtctgtac attgctttct aaagaatctt tccattgtgt
    14461 ctccgtcaag tcgatcagtg ttagagaaag accttgcata tctagtgagg tcataaaatt
    14521 accgactttt cggaaaacta cattgacgcc tctttcttcc aacagagcta aaacatcatt
    14581 catgaacacg aactgttcca tcaaaggcgt cccgcccatt ccgttgacta gtacacctgc
    14641 ttcttttggt aaattcgagt aatcatctaa aatcttagta acaagttcct ttgccagtac
    14701 tttagaaggc tgcatctttt ctcgacgata gcctggctca ccgtggatac caacaccata
    14761 ctcgatctca tcttctccta gttcgaatcc tggtttccct acttctggta cagttgctgc
    14821 tcgaagcgct agaccgatcg tcttagttgc ctgtacgacc ttttctccca gttctttcaa
    14881 ctcagctagg gaagcaccgt ttcttgctgc atcgcccacg attttgtgta caaggacagt
    14941 acctgcaact ccgcgttttc cagcagtata cgtactattt tcaacggcga tatcatcatc
    15001 tacgatcaca ctttctactt caatatcgtc catagcagct aattcctttg ccatatcgaa
    15061 gttcaatgca tcccctgtat aattcttaac gatcagcaaa actccttttc cactatcggc
    15121 ttccttaata gccgtctgaa tctgatctgg agttggagaa gtaaacacat caccaagcac
    15181 cgctgcactt aacatccctt cccctacaaa cccggcatgg gatggttcat gtccgcttcc
    15241 tccaccagaa accaaaccaa cttgttccgc tttctgattc ttggctacaa cacgggaaga
    15301 ttctacacga tgaacaagtt ctggatagct ttttactaat ccttccagca tttcttcgac
    15361 aatattgctt ggatcattca tgattttttt caatttattc ccatcctttc tttccggcat
    15421 tttgtgcttc tgttaagatt tgctgagtat catctgttat accggcagtg atggctgctg
    15481 caaaagctcc ttccactaaa ggcgcatcca ccagatgata acgttgctgc tgctcttctt
    15541 ccaacatgtc ttttgccagt tccgagttca ataccgcgct accgatatcc gcaaaaacaa
    15601 aataactagc tgcatcgctt gattcattga ctgcttctac gattttcatc gggtctgatc
    15661 cgatctcgcc atttattccg ccgagagaaa aaatttgcac ctcttcggat ttttgcatct
    15721 gctcaatcat ttctttaata ccatctgtta ctttttgact atgagaaata agcaaaatac
    15781 tttttttcac aagaatcttc ctttctttta catagttgct aacgattggt ttcgctttca
    15841 ttaatgaaca attcaagtat ataataaatt tctaattttg ggaaaatatg tgtagtgctt
    15901 cttgaagttt gttatacttt aattaaaaga gaggagtgat cgaatgatta caacaacaac
    15961 gaacagtgta gagggaaaag ccgtagtagc ttataaaggg atcgtttttg gtgaggtcat
    16021 tactgggatc catgcattta aagacttgga ggcggggttg cgaaatatct ttggcggccg
    16081 ctcaaaaagc tatgaaaacg aactaatggg tgcaagagaa gaagcacttg ccgaaatggc
    16141 cgaacgtgca aaagcattag gagctgatgc gatcattggc gtaaaaatgg actatgaagt
    16201 attaggcagc gacaatggca tgctgatggt cacatgtagt ggaacagctg tctccacgag
    16261 ataagtaggg gacatctgtt ttaataacta aagagagatg ggacatgtag tggcactcaa
    16321 gaattagatt tttcagtcta acttcgaggt actctacctt cccatcctct cttttttatt
    16381 tctttttgcg gatgattccg taagctgctg cgactaacac acccgcccct gctaaggaat
    16441 aactgacaga agctgttttt tcatttgttt ttggcaaagt ctgacttgtt gatttggatg
    16501 gctctgtcgt ttgctgaact gtttctgtcg tgcttccaga ggtcactgat ttttctcctg
    16561 ccgaatgcaa aatatctttt attcgttgga aactagctgc taagtcagct ttttttgctt
    16621 caaaatcatt agcaactgtt tgtgcttcgc tgatctttcc gtggattgct tgactggccg
    16681 ctgtgatatc cgcttgagtt tgacttagct ttccagaagc tgtttgagca acctgattta
    16741 attgctgatt gaattctttt gcttgttgga tcttcccgtc aagatctgtt tggacagctt
    16801 ccgcttttgc tttcgcttcg gcaagctttt tctcaagttg tgcacggtca tttcccactt
    16861 tatttttaag atcagctaaa tcttgatcga ttgctgcttg gtcttctttc aatttgtttt
    16921 ttgcagcttc taatgcttct tcgagttctt ttcttttatt ttccaagtct tgcaaatgat
    16981 cgatttggtc agaagaggta gaactattta ttgatgtatc ttctgaagat gaaatcgttt
    17041 ctcctgaatt atctgtcggt acattggaag tcatttctgt ttccgaggca ccgttttcag
    17101 caccagaaga gcttatggtt gttgaaggtt ctgtttgagc aggttcagaa gtttccgcaa
    17161 aagctgtctg tcccaataag aaaatactgg aaacagcaat tggccagatt aacggcttca
    17221 ttcttttttt catcactttt cccatcctta ctacttttta actaatttct tattataagc
    17281 aaaaatgttt ggaagaacca taaacacaac aggctgtttg tctaaaaaac ggtgcatctt
    17341 ttattttttt gtacgttccg ggcttttcca tgtcccgctt ccataaccta aaatcgtttt
    17401 gactgctgga atgaaagtcg tcacgatcgt tatcgcgttc actatccaga acaaaagcat
    17461 atatagtggt gcgaaaagaa ggtatttcaa ttttcttcgt cgatcatcta caatcagcga
    17521 agctagcaac tggaaaaatc ctgcgaccat ttcaaagcag atgaacacaa aactgaatgt
    17581 aaaagtaata taaatctgtt cgtagtttcc ttgatataca taatgcccga ttaatccagc
    17641 aaaaagaact acggaaatac aaaagaatat tgaccagaca atactcaatg tttgatcaat
    17701 gaagatgatc gttcttccaa tatgtttaaa aggatggagc atcacttttt tgaaattcgt
    17761 cagccagact tctgttccac cttttgccca acgttttctg tgtcgatata gcatcttcaa
    17821 cgtcacaggt acttccatga aaaagataat ctcaggagca aaaacagata accagtcatt
    17881 taactgatga tcccaagcaa tactgatgtc ttctgtcgct cgatcttgtc tgaatccgcc
    17941 cacatcgatc aaggcttctt ttcgatacat cgtatttgct ccactgtacg cataaagacc
    18001 accgaaaaca gctgactgtg ttcgtttaat gatgcccacg atacttgaga attcaaccgt
    18061 ttgagatttt gcaatcaatt tggtacgatt ttggacatcc atatttgcag tgaccgcagc
    18121 aatatgtctt gcaccagggc gaatgaaata attcacatac ctaatcagtg catctggctc
    18181 cggtaccgtg tctgcatcat tgcttaaaat caatttccct tttgcaaagg caagtccgat
    18241 attaaacgcg tgggcttttc ctttattttt ttcgatccga accacacgaa gattagcgta
    18301 ttttttcatc agtctagcta atatttctgg tgtttggtcc gttgaaccat cgtctgtcac
    18361 taatacctca tagttgtgat aattcaattt cgtcatcaaa tactcgattg tatattcgat
    18421 cacaatttct tcattatggg ctggcaccat gatcgtgatg aacggttcga cactcaaggg
    18481 aacatctacc cattcttttt gcttatgttt gtataaaaac acataacacc agacaccaat
    18541 aaaccataca aatccgccta aaatcggata agaaacgaga aaaaaataca taatagacaa
    18601 acaagtatct aagaataaat tacctgtatg ggtgattgag atcatttcag ttccacccca
    18661 ttttctttat acaaatctgc aatgaatgtc gtatctagat ttttctcttc ggagacagaa
    18721 taaaaacgga ccgtttctct ctcttctttt gttccaaaac gttccgtata cacttcttca
    18781 agtaattctt gtcttttttt caatttttca tcttcatatt caaaagattt atttactttt
    18841 ttgtatagat ttcgattgtt ccacctagtc aatacaaatg aggtaatcag gaaactgaaa
    18901 aaaatcaata caaaaaagcc gattaagaaa tacagcattt tcttttctga taaaaaagcg
    18961 tacaaggaaa aatccttggc tctttctggt ctcgttaatg ggaaaaagac ccatataaaa
    19021 ggaagaacaa cgatcatcca tcctaaaaga gccactaacg tttgccaaat ctttaaccag
    19081 taattacctt ttgaaaaata cttatctttc accaaaattg atgcaacttc ttctcttgtt
    19141 acttttttca tatcctcacc tcacgttttt ttgttacgac gtaggagttt accatacatt
    19201 tcatttcttt tcacataaag tgttcctaaa tttttcctaa gaaaaagaac aaaacatttc
    19261 tcatcagcaa atgaaaaata ttttgttctt tttatcagtt gttcttttta ttttttatct
    19321 cttaattatg ttgctctttc caatacgaca atgcttttca ttcctctaga tatcgccgtg
    19381 tacatttgtc tgcgtcttct ttgctcatct ccatcgtttt caaatggata taagattaca
    19441 tgatcaaatt ccagtccttt tgctagactc aaaggaatga tttggcagtt ttctaatcca
    19501 tatgaatttt ttttaatcga ttcttgtagc gtttgggttt cctcttctgt ttgggtgata
    19561 actgccgctt tttctccttt tagagaaacc aaaatctcag ctagtatttt caacaattct
    19621 tcagaggatc gaaaagagcg gtattcgggt tcttttcctt gcggtcggat cgatacgatg
    19681 tcgatcttct cttgatccac agcataggtt ttgaaaagct ctgtgattgc tccgctagaa
    19741 cggtaactat ttctaagatc ataccgatgg atcggcagat ggtagtcgtc gaaacaacgc
    19801 ataatatcag caaaagtagt ggaagaatta aagattgcct gattctcgtc acctactaaa
    19861 gtaaagtgag tttttggaaa taattcgatc aatagaccta attgtgccag ggtataatct
    19921 tgtacttcat caatcaatag atagcgaaat gctggtacat ttaccttttc aacaagtaaa
    19981 tgacgaatag ctaatataat gactgcttca tcaacagtac gtgtgggttg ccaagcataa
    20041 gctgagccat atcgtttttc ataaattctt tcaaacaact gatgctcatc cacccatgcc
    20101 atttcttcga tttgtctggt tatttttcga tatttttttt gtaaaagttt ccttgcataa
    20161 gctacaatac tttgttccga atcatcagaa atcaatttac cgaataattc caactgccta
    20221 tcttctgtca acgagagtac ctgattctgc acagcaggac tttttgcttg tttcaaaaga
    20281 tgattcttcc attcgctcat cagtgccttt ttcgttgctt gaagcctatc gatcaattta
    20341 ggatgagcag gaactttctc atatattttt tcgatcgttt ttttagaaag gactgttttt
    20401 ccatttttag taagtccacg gataaaattc ggatgatcta aaaacaaagg atctgattgt
    20461 tttagattat cgataaactt tttgctacgc aaacgttctg tctgctccga tacattttct
    20521 cccgtgatac gtttgaaata agcctcttca ccttctattt cctctgctga aagctgtgaa
    20581 accagctgta tgatggttag attcagtgga tttctttcac ctaatgatgg taggacattc
    20641 gaaatatagt cgataaaccg atgattaggt gatagaatca gcaagtcatc ggatgtcatc
    20701 gtatctcgat ataaatagag cagatacgca atacgctgca tgatagcaga ggttttacca
    20761 cttcctgcta cgccatttac caagatattt tgacttttca catcccgaat gattgtgttt
    20821 tgttccgatt ggatcgtagc agtgatagcc ttcatttcat tggtctcatc ttggcctaag
    20881 acttctaata atacgtcatc ttgaatagct acagacgtat caaaaaactt gaccaaacga
    20941 tctctttcca aaagaaattg tcgtctttct ttaataaccg tatcgatttt attttgacga
    21001 accgtataag aagatggacc aatcatgtta ttgtaaaaaa gttcggcaat tggtgaacgc
    21061 caatcataaa caagattttc atctgcatca tctgtgaaat ttgctgtacc gatataaaag
    21121 gattcttttg gctcattatc cagaaagtct acaacgactt ttccaaagta gggacttttc
    21181 aataaacgtt cgatctttgc tagctgattt tctgcgtttt tctgtttgat attcatctga
    21241 tcgatttctc tatttttcat ttcaatcatc gaaaaagtat ccaggttatc taagtaactg
    21301 tcaaaattca atcgaagctc gccagccatt tctttcaacg actggtttcc ttctattttt
    21361 gccgtatcca tcgattcccg cagttcgttt tttttatcaa ttagcttttg atacacgttc
    21421 ttcagatatg cttcttctaa ctgtttttgt gtgttgacca tacacattct cctctttttt
    21481 taaagtggtg gaaccgatta gtttacctct tttaagagtc gaatgcaagc tttttaacga
    21541 gtatcatttg ttaggatata tcagttcttc aggtcggaca gtttgatata aagaaaatgc
    21601 accatcaagg ttttttacgt ggtagccaga ctgcttcaag attctttccg ctagatagct
    21661 acgtaatccg ctatgacagc tgacgatata ttcttgttgg ctgtctaatt catttaatcg
    21721 gtcccgaagt tcattcaaag ggatatgttt ggcttccttg aatttgccct tttccaattc
    21781 ttctgctgtt cgcacatcta ataaaatttt tccttttgct agttctgtcg gcaattcatg
    21841 ccattgtacg gtttcactaa gcccttctgc aatgttcatt gctgcatatc ctagcatgtt
    21901 tactggatct ttggctgagc caaatggcgg tgcatacgtc aattctaatt ccggtaaatc
    21961 gaagatcgtc aaatgccctt tgattgctgt tgctaaaata tcgatccgtt tatccacacc
    22021 ttttgcccca acaccttgtg caccataaat ctcgccagtc tctggatgga agatcaactt
    22081 cagtaaaata tctgttgctt ctggataata gcttgcatga tcttttcctg atacatgaat
    22141 gacttcaaaa gacagttcgt tttctttggc tgttctttca cttaaaccgg tggatgcggc
    22201 agtcattcca aacgttcgta taattgctgt tccaataccg ccttgatttc ttcgagaaat
    22261 tccggaaatc gtgtccgcaa cttgtctacc ttgacgattg gctggtgaag caagagaaat
    22321 aagtgcatct tttccagtga tttgctgttt tacaacgata gcatccccca ccgcataaat
    22381 atcaggaata ttcgtttgat aacgttcgtc tactaggata cccccgcgta tgcctaattc
    22441 gattccagcg gtcactgcta gctggttatc aggacgaaca ccaatggata aaataactaa
    22501 atcagcaggg atggtacgcc catcatccag tcgcaatcgt ttcccgtggt cttcaaatcc
    22561 agcgacagca tgagaagtaa tcacttctac tccttgatga gacaattctt tttctataaa
    22621 agctgccatt tcttcatcca gtgttggaag aatatgtggt gcttgttcga tcaccatgac
    22681 ttctaaacct cttcttttta ggttttctgc catttccaat ccgatgaatc ctgcaccaac
    22741 gatcacggca cgctttggct gtttttctaa tgcatgcatc actttatcga tatctggtac
    22801 atttctaata gaaaaaacat tgtcggcttc tgttatccct ggaatcgatg gaacaactgg
    22861 ttttgcacca ggagataaaa ccaatgcatc gtactgttca atgtgttttt gaccctcatg
    22921 tacgacttct accgttttgt tttcgggaaa gatggctgtc acttcatgat ttggacgcac
    22981 atctaaacaa aaacgagcgg ataatgattc tggcgtttgg acaagaagat tttcccgttc
    23041 actgatttct cccgaaagat aataaggcaa gccgcaattt gcaaaagaaa catacggtcc
    23101 tttttcaaaa acaacgattt ctgcatcttc cattaatcgg cgaagccgtg tagcagcaga
    23161 cattccacct gctacacctc cgacaataac gattttcatc ttatttccct cctactgttc
    23221 gatcaaacca ttgattcatg ccaccacgta cattcactac atcataacca gatttcttta
    23281 attctttagc tgcttgttta ctcctcatcc ctgattgaca aatcacatag acggtctttt
    23341 ctttgtctcc atcatatctg tttatacttt gtaatggaac atttttagct ccttttatat
    23401 gcccgccccg gtattctgca ggtgttctaa catcaagtaa cgctggattt tccaaaagct
    23461 tttcctttaa ttctgggact gttatcgatt ttattgattg aaatagcaaa ttcattttct
    23521 gcacctcatt tacattttta taaatacctc atggggtata ttaaacagga gctttattaa
    23581 tatgtcaacc agaatgactc ttcatttttt tcactacttt ttatatttgc ataaaatacc
    23641 gataaggggt attatctaat tataaaaata gaggagaatg tgttatggag attgataata
    23701 aaaatatcct gaaccgtttg aaacgtacag agggacaaat tcgtggtatc caaaaaatga
    23761 tcgaagacga aaaagaatgt atggatgtca tcactcaatt aagcgctgtt cgttctagta
    23821 ttgatcgcgt aatgggcatg atcgttgcag ataatctgaa aaattgcttt gagaaacctg
    23881 atagtaatcc agaagaacaa gcggctaaac tagaacaagc gatcaagatg attattaaaa
    23941 agtaaaggaa tgatttcaat gaaagtcgtt ttccatattg atgaattaga aaaatggtca
    24001 gaaacaggga aaaacgtaaa aaacctgata aaagcttctc cagaaacgac gattgtcgta
    24061 tctgtaaatg gcatcgcgat taccggttat ctagactctg ccaatgcaga attcttagat
    24121 ttgcaaggag tcgtttttca cgcttgcgcc aatgctatgc gtgctaatca tatttcagaa
    24181 agttctttac ctgaacaagt aatcgttgta ccagcaggtg tccttgattt agttgaactg
    24241 caatcccaag gttatgctta tatcaagcct tagtcagtag aggttgcgac agaagtgatc
    24301 agcttcaaga aatttcggct tatttttcga agaagctact tctggaaaac cgtgaatcaa
    24361 aggttgtgaa aa
//
LOCUS       DBNICI010000028        23828 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.028, whole genome shotgun sequence.
ACCESSION   DBNICI010000028 DBNICI010000000
VERSION     DBNICI010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.028"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(53..787)
                     /locus_tag="AC1ZCC_000903"
     CDS             complement(53..787)
                     /locus_tag="AC1ZCC_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HIQ1629174.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="AC1ZCC_000904"
     CDS             1114..1524
                     /locus_tag="AC1ZCC_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HIQ1629175.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="AC1ZCC_000905"
     CDS             1517..2218
                     /locus_tag="AC1ZCC_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HIQ1629176.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="AC1ZCC_000906"
     CDS             2218..3804
                     /locus_tag="AC1ZCC_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1629177.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="AC1ZCC_000907"
     CDS             3823..4044
                     /locus_tag="AC1ZCC_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HIQ1629178.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="AC1ZCC_000908"
     CDS             4041..4802
                     /locus_tag="AC1ZCC_000908"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HIQ1629179.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="AC1ZCC_000909"
     CDS             5182..5691
                     /locus_tag="AC1ZCC_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HIQ1629180.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="AC1ZCC_000910"
     CDS             complement(5766..6305)
                     /locus_tag="AC1ZCC_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HIQ1629181.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="AC1ZCC_000911"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="AC1ZCC_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIQ1629182.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="AC1ZCC_000912"
     CDS             7318..8169
                     /locus_tag="AC1ZCC_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HIQ1629183.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="AC1ZCC_000913"
     CDS             8197..8832
                     /locus_tag="AC1ZCC_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HIQ1629184.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="AC1ZCC_000914"
     CDS             8852..9625
                     /locus_tag="AC1ZCC_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIQ1629185.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="AC1ZCC_000915"
     CDS             10033..10830
                     /locus_tag="AC1ZCC_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HIQ1629186.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="AC1ZCC_000916"
     CDS             10808..11221
                     /locus_tag="AC1ZCC_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HIQ1629187.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="AC1ZCC_000917"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="AC1ZCC_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIQ1629188.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="AC1ZCC_000918"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="AC1ZCC_000918"
                     /inference="COORDINATES: protein motif:HMM:NF000950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIQ1629189.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="AC1ZCC_000919"
     CDS             15998..16558
                     /locus_tag="AC1ZCC_000919"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIQ1629190.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="AC1ZCC_000920"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="AC1ZCC_000920"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HIQ1629191.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="AC1ZCC_000921"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="AC1ZCC_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HIQ1629192.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="AC1ZCC_000922"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="AC1ZCC_000922"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HIQ1629193.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="AC1ZCC_000923"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="AC1ZCC_000923"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIQ1629194.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="AC1ZCC_000924"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="AC1ZCC_000924"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIQ1629195.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="AC1ZCC_000925"
     CDS             21905..22483
                     /locus_tag="AC1ZCC_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIQ1629196.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="AC1ZCC_000926"
     CDS             22535..23017
                     /locus_tag="AC1ZCC_000926"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HIQ1629197.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="AC1ZCC_000927"
     CDS             23134..23508
                     /locus_tag="AC1ZCC_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629198.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7781 a         4286 c         5115 g         6646 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaca
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttt
//
LOCUS       DBNICI010000029        22346 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.029, whole genome shotgun sequence.
ACCESSION   DBNICI010000029 DBNICI010000000
VERSION     DBNICI010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.029"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            156..278
                     /locus_tag="AC1ZCC_000928"
                     /pseudo
     CDS             156..278
                     /locus_tag="AC1ZCC_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
     gene            392..631
                     /locus_tag="AC1ZCC_000929"
     CDS             392..631
                     /locus_tag="AC1ZCC_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629199.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            743..937
                     /locus_tag="AC1ZCC_000930"
     CDS             743..937
                     /locus_tag="AC1ZCC_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629200.1"
                     /translation="MEEKLLKTMKQKHLKRLSVMQYINDMQITGKEKACLLGSMKNFE
                     QLRRTYVKTSSNCQLLLEVS"
     gene            1023..1694
                     /locus_tag="AC1ZCC_000931"
     CDS             1023..1694
                     /locus_tag="AC1ZCC_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HIQ1629201.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQQEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1747..3723
                     /locus_tag="AC1ZCC_000932"
     CDS             1747..3723
                     /locus_tag="AC1ZCC_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HIQ1629202.1"
                     /translation="MAINRYVKKAGMLIGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            3738..4490
                     /locus_tag="AC1ZCC_000933"
     CDS             3738..4490
                     /locus_tag="AC1ZCC_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HIQ1629203.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLESNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            4506..5261
                     /locus_tag="AC1ZCC_000934"
     CDS             4506..5261
                     /locus_tag="AC1ZCC_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HIQ1629204.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKNRVKKLSSDQLKLVSNEETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            5274..5534
                     /locus_tag="AC1ZCC_000935"
     CDS             5274..5534
                     /locus_tag="AC1ZCC_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629205.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            5531..7621
                     /locus_tag="AC1ZCC_000936"
     CDS             5531..7621
                     /locus_tag="AC1ZCC_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HIQ1629206.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            7648..8112
                     /locus_tag="AC1ZCC_000937"
     CDS             7648..8112
                     /locus_tag="AC1ZCC_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629207.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            8109..8351
                     /locus_tag="AC1ZCC_000938"
     CDS             8109..8351
                     /locus_tag="AC1ZCC_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HIQ1629208.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            8369..8671
                     /locus_tag="AC1ZCC_000939"
     CDS             8369..8671
                     /locus_tag="AC1ZCC_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HIQ1629209.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            8741..10804
                     /locus_tag="AC1ZCC_000940"
     CDS             8741..10804
                     /locus_tag="AC1ZCC_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629210.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            10804..13416
                     /locus_tag="AC1ZCC_000941"
     CDS             10804..13416
                     /locus_tag="AC1ZCC_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HIQ1629211.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            13413..15317
                     /locus_tag="AC1ZCC_000942"
                     /pseudo
     CDS             13413..15317
                     /locus_tag="AC1ZCC_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
     gene            15435..15944
                     /locus_tag="AC1ZCC_000943"
                     /pseudo
     CDS             15435..15944
                     /locus_tag="AC1ZCC_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
     gene            15994..16326
                     /locus_tag="AC1ZCC_000944"
     CDS             15994..16326
                     /locus_tag="AC1ZCC_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629212.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            16327..16920
                     /locus_tag="AC1ZCC_000945"
     CDS             16327..16920
                     /locus_tag="AC1ZCC_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629213.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            17083..19107
                     /locus_tag="AC1ZCC_000946"
     CDS             17083..19107
                     /locus_tag="AC1ZCC_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HIQ1629214.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            19107..20312
                     /locus_tag="AC1ZCC_000947"
     CDS             19107..20312
                     /locus_tag="AC1ZCC_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HIQ1629215.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPFLNSSKK"
     gene            20328..20972
                     /locus_tag="AC1ZCC_000948"
     CDS             20328..20972
                     /locus_tag="AC1ZCC_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629216.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIEQLKQQDGTKAKEEVTEAQSKTAEEFG
                     KLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            20983..21789
                     /locus_tag="AC1ZCC_000949"
     CDS             20983..21789
                     /locus_tag="AC1ZCC_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629217.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTHQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFAVKLSHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            21811..22041
                     /locus_tag="AC1ZCC_000950"
     CDS             21811..22041
                     /locus_tag="AC1ZCC_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629218.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
BASE COUNT         8253 a         3506 c         4594 g         5993 t
ORIGIN      
        1 atcaaataaa caagaaccgt aggaactacg gggatagctt ggtaaataag aggcacctct
       61 gttagtaaag taatacgcta tcaagtatgc tctcttccca agaagctccc acttcaagcg
      121 taagaaaccg ttaggtttag ctaagtggtg agtagttcac aatgacgctt acgaatgata
      181 atggagacga attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagcg
      241 ttgaaatcat tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac
      301 agataaaggc ttagaagaag tcacgacgaa taataaaaag gtagtattac cttaggcatt
      361 gagtaaggaa ttcatttagg aggaaaatta gatggcgcga ccagttttag aggcgatgga
      421 tatggatact ttgacaaaag gaaggtatgt acagctgtta gctcgtggtt attcacctaa
      481 agatgtattc aaatccctgt ctaaaggaac agatatggaa aaagaaagat tacgaaaaga
      541 attcgattat tggagaaccc ataatggaat caaggatttg aaaccagcac ggccagtaat
      601 taaacgaaag aaaaagataa cccaagactg aagtgtagag cgtatgtgat cataggcttt
      661 tttatttttg caaaaaacaa ggaaggtagg aaaactcatg aattatgaag aaatcgaaaa
      721 cagaaaaaaa gtgtcgaaat aaatggaaga aaagttgcta aaaacgatga aacaaaaaca
      781 ccttaaaaga ttatctgtca tgcaatatat caatgatatg cagataacag gaaaagagaa
      841 agcttgtttg ttaggttcaa tgaaaaactt tgaacagttg agacgaactt atgtgaaaac
      901 aagttctaat tgtcaactat tgttagaagt ctcatgacat tcattaatag aattcatttt
      961 taaaactttt tctatggaaa tgagaaaaag attttttgtt ttgtctgaaa gggggtagct
     1021 ggatgagaaa atggttattt ggttttttag gagtcgcttt aatcgtcttt tgcagtgtgt
     1081 ttggctatgt tagctaccaa aaacatgaag gagaggtgtt taagcaaaat atagaaaaaa
     1141 agatgcccgt agatcaaatc aatgcacatg caaagagtta taaggaggat gcaacgaacg
     1201 tcaataatga tatgtctttg gggcaaatgt tgtccataca acaagaagca atcgaaatgg
     1261 gcgtcaataa acaagtattt gcacaaattc agattcctgc acttggttta gcattgccaa
     1321 tttttaaagg ggcaaatcaa tacactttga gtcttggagc ggctacttat ttttatgaag
     1381 atgctgaaat gggcaaagga aactatgtcc tagcaggtca taacatggaa atgccaggtg
     1441 ttttattttc tgatattcaa aagttgtcat taggggaggt gatggactta gttagtaacg
     1501 atggcgtcta tcgttacaag gtaacgcgta aatttatcgt acctgagtac tttaaattaa
     1561 tcgatggcgt tcctgaagag aactctttct tgtctttgcc aaagaaagga gagaaaccct
     1621 tattaacgtt gtttacttgt gtttatacgt cacaaggaaa agaaagatac gtggtacagg
     1681 gagaactaca ataacaaggt tttaatcaac gtgagtcgtt gaaatatgaa atgaggagag
     1741 taaaaaatgg caataaatcg gtatgtaaaa aaagctggaa tgcttattgg aatgttagga
     1801 atcattgcag gctgctttca gggatatcga gcatatgcag aagatgtcac gcaaaaaacg
     1861 ccaccagaga aggtcaatat cacggttcat aaattaatgt acgatcaagg aacgcaattg
     1921 aatgttgata ttgacgggat caaaaacgat ggttatacgc acgatcaata tcctacaggt
     1981 gtaacgaaat acaacaaggc agattatggt gatgttgagt ttacgttagg aaatattacg
     2041 gatcaagtat taccgagaga agattctgat ctaaccaacg cgaaagtgga cgagattgta
     2101 caagatgtag aagacaaggg atctaacagt gagtacgtca agaatgcaac caacaaaatt
     2161 acttcagctg tagatgaaaa tggtgagatc acttttactg atcaaccagc ctatgtcaat
     2221 tccaaaggga atgtatatgt ggtttatgaa agtaaatccg ctgctggatt agtcacacaa
     2281 aaagcaaaac caatggttgt gattgcgcca atgacagata aaacaggatc aggattttta
     2341 aaaaatattc atttgtatcc taagaacatt gtttctaagt tgagttttga attaacgaaa
     2401 tttggtgacg atggcgcaga ccaaagcaaa caaacccttt taaaaggggc aaaatttgag
     2461 ttatacaagg gcgaacctgg aaaaggaaca aaattgggtg atcttgtatc ggatgatcaa
     2521 ggaaagctga ctgcgactga tttgacgtta ggaaaatact attttgtgga agttccttcg
     2581 gaagttgtag tgggatcaga tcaagaacca acagcagatc aatatttatt aggcgcagac
     2641 gctcgcaacg atgcgcacaa taaattaacc tttgaaatta cgaatgatgg cgtgacaagt
     2701 gacttaaaag cttcatacgt aaactataaa gcaccagtga tcgataaaac agtaacaaat
     2761 ggaacaggac aagaacattc tttccaaatt ggtgacgcag taaattatca aggaacgatt
     2821 catattccaa cagacattgc aggtggcgca gacggtatca cagtaaacgg tgtgaaaagt
     2881 gaaacttctc cttacagtgt atttaaatgg ggagatacag caggacaggg actttcttat
     2941 gtcgctgcta aagcaaatat caaagtaaca aataaagatg gatctgtggt attaaaagaa
     3001 aatacagatt ataaaatcca aaattcagaa aatggctttg tgatcgattt tatcgtgaat
     3061 aatggccaag taagtgatac tgtagcaagt ttacacggac aagatcttca aatgacttat
     3121 aacatgtatg taaatgacag cgcagctgtt gccaatccat taacaaatag cgtggatttc
     3181 gtttataaca acaatccatt taatcaggaa gaacaccacg aaaaaacgaa agctgacgtt
     3241 gtgacttatg gtgcaaaatt cttaaaagtc gatagcggac tatttggaac aggaatcaaa
     3301 gcaacaccgc ttgaaggcgc agaatttgct gctaaaaacg cagaaggaaa atattatggt
     3361 ggattagttg atacggataa agacggtgtc aaagaagcag tatgggtaga cgatgtagca
     3421 aatgcagcga tcctaaaatc agataaagaa ggtcactttg aaatcactgg tttaactgaa
     3481 ggtgaatatt cactggaaga aacaaaagcg cctgaaaact atcaaaaatt aacgaaagag
     3541 atctctttca aagtagacaa agattcatac aaagaagaaa accgaatcac gatcaaaaac
     3601 aatcaaaaag cttccgtacc aatgaccggt agcaacggtt ttcaaacgta tgtattaatc
     3661 tcttgcttgt tgctaggagc aggggcactt tctgcagttg tttactttaa gaagaaagcc
     3721 tagaaggatc aaaaccaatg attatcagga agcctaagaa aaagcgaatc atgggtaaat
     3781 ggatcattgc tttctggtta ctttcagctg taggtgtctt acttttgatg cctgctgaag
     3841 cgagcgtggc gaaataccaa caaaatcagc agattgccgc aatcgatcga acaggaacgg
     3901 cagcagaaac ggattcttct ttggatgttg ctaagattga gttaggcgat cctgttggta
     3961 ttcttacaat tctaagtatt tctttaaaac taccaattta tgacggaacg tccgacaaaa
     4021 ttttagaaaa tggtgtaggt attacggaag gaactggtga tattacagga ggaaacggta
     4081 aaaatccttt aatcgcaggt catagtggac tttataaaga taatcttttt gatgatttac
     4141 cttcagtaaa aaagggagaa aagttttata tcaaagttga tggtgagcag cacgcttatc
     4201 aaatcgatcg gattgaggaa gtgcaaaaag acgaactaca aagaaacttt gtcacttact
     4261 tagaatcaaa tcccaatgaa gatcgagtaa cgctaatgac atgtacgcct aaggggatca
     4321 acacacaccg ttttctagtg tatgggaagc gagtaacctt tacaaaatca gaattaaaag
     4381 atgaagaaaa taagaaacaa aaattatctt ggaaatggct tttaggaagt actgtttttc
     4441 tttcagtcat gatcattggt tcgctatttg tttataaaaa gaaaaaataa aggagggtaa
     4501 ccaacatgag aaatagaaaa tggctatttg tttgtgcagc cgctcttttt tctttcactg
     4561 cctacacaca gttgacgttt gcagacagac aagaaagcac ggatagcaca gaacaaacaa
     4621 tccaaacgtc agatagcagc acgattcaaa gcagcgatga aaccgtcgat tcgtcaacag
     4681 ttgattcttc agaagaacct aaaattgctg aagacgatca aatagtcaaa cagaccattc
     4741 acgttcaaaa gattatttct tcagaacaat tagacaacca ggggcatgaa aaacaagaag
     4801 gagtaaatgg cgcgaagttt tctgtttatg atgtatcgga cattttacaa aagatggatg
     4861 ttaaggatct cactactgac caaattgaat cccaattaaa aaatcgagtg aaaaaactat
     4921 ccagtgatca attgaagcta gtttctaatg aagaaacaaa gacaattgat caacaaacag
     4981 gtgtctttga attctcggta gaagtacagg caaatcagaa acaagcttat tatattgtca
     5041 atgaatcaag tcctgaaaac atttctaata gcgaggatat tttgctatta acacctgttt
     5101 ctgataaaaa cggtgaattc ttaaaggatg tgtggatcta tcctaaatca gaagcttcgc
     5161 aacctaaaga agaagtaaaa aaaatcgttt ctactggcgt aaagaaaaac ttctttgaga
     5221 attgttggga ttttgttact cacttgtttt ctagagatta aggaagaaat aaaatgaaac
     5281 tgatcaaatt attattattc aatttttctt gtttaaccgc agtctttttg tactgcggtt
     5341 tattattttc taaactatct attttgccaa gctaccaact atacctaatg aggattttaa
     5401 acataccagc tgcttccatg ccgctagata ttatggaaaa gctcattagg aatccattat
     5461 ttactacgat caatttgatc gttattgtcg gaatttcact agtcatttat aaaaaagagg
     5521 ggatgaaggg atgaatttta aacactcgtg gacaagacgg ttaaaatatg tttttcccat
     5581 aatgatgatg ttaggtacat tgtttgggtt aaaaacagcc aatgtctttg ctgaaaaagt
     5641 tattgtcgat ccgtcaaatc caatccagaa cgtaaagaat aattttatta ttccaaaaga
     5701 tgttcctaat gcaaaaacaa atattactgt aaacggagcg tcaaaagttt acaaatatca
     5761 tgttgatgcc aacaaaggtg gttttacaga cgactacgta ggattagtgg agggaaacaa
     5821 aaatatacgc tatccttctt tccaaaaaga atacggtgaa acaaaccttg ttttagaagg
     5881 cgtaacaacc aacaccaaag tcaatattga ctatggaaaa atcggtacgt ataacgggaa
     5941 aaaagtcaat atcaagctag ttctatctaa tattcactta tactcggata cgttgccgtg
     6001 gaatctacta gataacaact atacaaaaac tcattttcga gacgatggct ataaaaatac
     6061 gaatggtgca atgagtaaat ctaaaaaaag aacagtactt tggatctcag acaatctttt
     6121 ctcaggaatt gtttaccatt caacacaaat gaatgtgcag ctagtagcca catatgagga
     6181 cggtagcccc gttcaatttt caggagatac atttatcagt tttaactctt tgaaccctgc
     6241 aggaggaaaa agcacggatc ttaaaggaga atatgcccac tatgacaaaa tgaatacgac
     6301 agattggtat gtcagaaaag atacggttct ttcagaattt aaatctttct acaacaatct
     6361 gaacgtggta ggtggccacc caggaggaag ctctaagctg acacaagcgg ataatgattt
     6421 caataatttg catgataaat taggagatcc gaagtttggt caaggtactg tgtcctttaa
     6481 aatatctgaa gcaaatccga cgtttgtgat tggttcttct aatgttcaaa cctggtttac
     6541 tttatcttct gcgacaatct ttagtgttgt tcctgatcaa cctgaaaaaa caggggttga
     6601 taaaaatgga aataacgtga acgataaaat gttgcaagta ggagacacga ttcaatatcg
     6661 aatcaaacag aaggtgaatc gattaggagt ggatttacta gcggtttacg atcgatttga
     6721 gttaatcgat aatttaccaa aagaagtaaa ttacgtcgat gcacacgttg aaagtggtac
     6781 aaataaaaaa ttcgatgtat ctggtgaagt gacctatgac aaaacaaaac accaggtaaa
     6841 atatgcagcg aaagctgata cattaaaaaa tcgaatgaag tataacggcg aaacttacga
     6901 attagtgatc aatgtaaaag tcaatgaatt ggccaatcaa aatagtgtag ccaaaaatca
     6961 gggaacgtcg atcattaata aagtagaaaa ggatacaaat atcattaccg tttatttccc
     7021 taaaattcca gtgaaagaag tacaacaaaa tggaaaagat gtaaacggac gaaatgacgg
     7081 taagaagggt acgccgacag ctcctttaaa cgcaggatct gaagtacaat atctagtcac
     7141 acaaaaatgg cacacaaaag gcgtagacgc tgcgtcagat cactacaaac aatttagtat
     7201 tcaagatcca attgaagctc gtttgacata taaagaaggt tctgcgcaag taatagataa
     7261 gagtaaagga aaagatatta cttctgaagg cacactaacc tatgatagca attcaagaac
     7321 gctgaagtgg gaagcttccg ctgacttctt aagtaacaat cttttagatg gacgagaaat
     7381 tcagctgata tttacagcta aaactccatt acaatcagaa aaaaatattg ataaccaagc
     7441 cgtagtagca gtagataatg tttctaataa aacaaatgtt gtgacaattg gggttgatcc
     7501 taacttgcca caggtcattg ttcctaaaac aggttctaca catttagtaa cgatttcagc
     7561 ggtcagctta ctattacttg tgttagcaac ttttagttat gcggttctga gatacattta
     7621 aaataaaaaa acgaaagaat gaataaaatg aaaaagttaa taatagtagt tacttccttg
     7681 attgccatga ttgttatttt agtcggatgt agtaatgaaa aaaataaaca aactaatcaa
     7741 gataatggcg tattgaaatc aggaactatg tggaaagaag aagtaggtgg tcttgtttat
     7801 aatttgaaaa ttatagacga gactacctgg gaatactctg agagtgtatg gcatccagat
     7861 cctgtacaaa taacagtcaa gaggcaaaaa gactataaag gtttagaaag atacaaaata
     7921 gtagattcag ctggtgttag ggaatttata aacaaatctg attctttatt tattgttgtt
     7981 ccctatgaga agaatggcgt aaaaaaaata atttttcttg gatctagcaa ggatgaaaag
     8041 cgaacgaaag agaaattgat tcacgatgga tcacaatcca ataaatataa gttacaaaag
     8101 actagcgagt gattgctagt ctttttattt gtcctgaagg aggaatcaca gtggcaaaag
     8161 ctaaaacgcc atttgaaaaa gccgcgtcta aaatcttaga acgccgcggt gtatctgaaa
     8221 cacagtggaa acaagaagtc cttgaaaaag cacaatttga cctattacgg ggaaaagaca
     8281 aggacttaga aggatatatc tatcaacgtg aacgtgaaaa gcttgttttc gatgaaatta
     8341 ttaaacacta aaaggagcga acaattttat gtttgaatca attacaatga attttttaac
     8401 acaaccgaaa gtagtagctg cggttgatat ttttagagca ttacaaactg ccgcaactag
     8461 tttaaaaaac aatctgatga tttttgctgc tgctgcggcc gttgcttcca ttgttattgg
     8521 gggtcttttc ttcctatttg gacgaagagg agctgaaaat gggaagatgc acgtaggcta
     8581 tgtattgatg ggggtaggag ttattttagc agcagcttct attgtggttt cgttatttgg
     8641 attgtttgga caaacacctc aaggattata agaaagtaaa aaagtaggag taagaagtag
     8701 tcgatacttt ttactccttt tttatgaaag gagaatactc atgtacttta cgattgcagg
     8761 caataccaaa acagataaag agttaataaa aaaagcaacg aaggaagtag gaaagcgtac
     8821 ttctttttta ccgaaaacaa ttaaatattc tgtcattgaa acggaagaac agctacatgt
     8881 gttagggaaa gtactggaac acgaaagtcg atcttctgtt aagttgactc tcacgttaca
     8941 tgacacagaa ggaaaaatag agaaagctgg tacagaatac gtttttggcc cactggatta
     9001 tcaaatttca gaaggtttct atcctacatt gatggattta atccgccttg atgtgtttaa
     9061 agaaacggat gaaactatga gtgtggaaga gtcttatcga aacgaaaaac ttttacaagt
     9121 tttagaagaa gagttagaga gcgaccaaag aaaagtgaat caagggagtg aagtcgaagt
     9181 aaatgaagaa gtaccatggg aacgaccaat atttccaaag aaggagtcaa aagaaaaaga
     9241 gacagtctat gttgacgaac aagctgatat tccgactcag aaatcgatat ttgaggaaga
     9301 gaacgatgtg atgggtcaat tggaggtaga tccctttgaa gaaacaatgg aggatgaaga
     9361 gacggccgaa gtagaagttg atccagttga agcgaatgaa gagtcaacat ggactgaagg
     9421 ggattctcag gaatatattg aaccacttat cagtgaagaa gtagaaaaaa acgaaataga
     9481 gatacaaaag gcaactgtat ttgattatat tttggatcaa tatccgccag agcatgaatg
     9541 gatgaaagaa aaaaacaaat tatttttaaa tgagctttat tcgaaatatc aattacccca
     9601 aacctatcaa gcatttttag agagtaaaga agaattaatc tcacagggta aaggcacact
     9661 tttagaacag ttagagtctg tccaatcaac aaattggcat gagcttgcac aagaagattt
     9721 attagccacc tttgaagaac gtcaacaaga aacagatcaa gaaattaatg agtatgcgac
     9781 ggaacagtcg tctaattggg agaaagcaaa agaggaacta aataaagaag aacagcgagt
     9841 gattgaagaa gaagtacaaa aaattaaaga gcgatacgaa aagaaacgcg acaatgcgta
     9901 ttccttgtgt gtagcaaaga tcaataattt tactcagacg aatcaagaag cgttatcgaa
     9961 agaaaaagaa aacttgttaa aagaacgtat taaagaacga aaagaagaat tttaccgagg
    10021 tttgcgtaca gataagctaa agattagcaa agaattaaat gaacaactga ataagctata
    10081 tcaaacaacg actgaagttc ttatggaaaa atacgaaaag atccagcaag aattacaagt
    10141 gcaaattcca aaatggaaaa aagagtatga aattcaacaa caagaattga aagaacaaga
    10201 agaaaaacaa cggcaaaaag aaaaagagcg cgaacaatta gaactaaaaa aacgtgaaca
    10261 ggatcttgcg gaacaacaaa tggcaataga aaaacaacgg ttaaaccaag aaaaggaaaa
    10321 agaagcagct gctcgtaagg aacgtcaaga acaaatggat acattgcgac aagctcagtt
    10381 aaattatcta gcacaacctc aaccaacgcc ttcagtagct gctgttcaaa ccccaatgaa
    10441 caaccaaaca aatctaactc cttcaccaca gcgttccttt aacggatgga tggtgggatg
    10501 tattgcaagt ttaagcttac ttctaggtgg aggaacagta tttgctttta atcatatcaa
    10561 tcaatcttca caggctgatt ctgtggccac aacgacgtca atcaaggaag aagcccaaga
    10621 acaagcaaga aaagagtatg aacaacaatt agaagaatta ttgaatcgta ccaatcagtc
    10681 aacaacagag tcaagcatgg aaaatagcaa agagagtgat tctagtaagg ctaaagaagg
    10741 atcaagcgat tcaactgtac aatcaaacac ttccgaaagt tcagaatcaa atgaggtgaa
    10801 ataatgcgtg ttagagaaac aacgaagcga aaagacaaac gattcaattt agaaaagaat
    10861 aggtatcttt ctcatttcta tcgctatact ccctcaggag aagagctacc agaaaaacat
    10921 gacaaacgca tttcgaaaaa gattttttat agtggacttg tatacctccc tttagagtat
    10981 ttagtaacaa gagtaccaga agttttcaac cgattggaaa ttgttaaggg aagcttacaa
    11041 ggaataaatc tgtttcaatt acaggaaatg ccacaggtct ttcattttga ttcagctggt
    11101 ttttggaatc catttactcc tacaactagt ttgggtacac gtttagcaat ttcgacagtt
    11161 tgtgtggttc cagctgtact tatgggattg aaaatggaca tgtggtttcg tcctctagca
    11221 gatggacaaa aaggggataa tcgattaatg accttaaaag aaatccaaca gatttatccg
    11281 caaatacctg aaacaaaaac tacttttcca ggttatggag gtattccaat cacacattac
    11341 aaaaaatatt ggtacattca aacagatact gttaatacgt gtattgttgg tacctctcga
    11401 tcagggaaag gtcagattga agtgttagcc accattgaca atttatctcg tgcagaaaaa
    11461 caaagttcaa tggtcgttaa tgatccaaaa acagaattat atgttggtag taaagatgaa
    11521 ctagaagcta gagggtatga tgtatatgcc tttaatatca ccgatccact gcaatcaatg
    11581 agtgataatc cattggcact catcgtgaaa tactggaaac gaggagacat agatactgca
    11641 acacagttaa ccaatacttt cacaaatacg atttattatg atgccaacgc ttcagataac
    11701 aaatacttta acgaaaacgc gcagaaagct gtcaatgcgc ttatttttgc gttgttagaa
    11761 caagctgatc aaacaggaga ttactccaaa ttgacaccga ataatctcgt tgagcttctt
    11821 tctgagattg gcggttttaa ttattatcca gatccagaca atgaattcaa acagttgaat
    11881 gccttagatg aatttatgaa tcagttgcca cctggaaaca ttgcaaaaaa acaatatggg
    11941 gcaacgaaaa ttggatctga taaagcgaag ggaaatattt tatcaacggc aatcacggga
    12001 ttgaatccat ttactctaac gaaaattgca aagatgactt ctcaatcaac gattgattat
    12061 aaatcggttg ggtttccaaa atacttagat ttgaaattac atgaaagtct tttaaatcaa
    12121 cgagtcgtag tagaatttta tcataaggga aagaaatttc atgaagaacg tgtcaaagta
    12181 ggctataaag gattttgtga aatcaatttt gattgtcatt tagttgcagg tgatcaagtt
    12241 tgtttaaaat acgatcatca aggaaaagaa tatgtggctt ggtatgaatt ttctcaaaga
    12301 gtattgaaag acgaagaggg taatatcgtt tatcggaaaa aacgaggaga aacccatttg
    12361 ccagaattag aaaaagaagc cgttctaaca ttagatgagg agaaaagtaa cttattagta
    12421 gagtctattc ggatgcacta taccgataaa ccaatggcta tttttatgct tacacctgat
    12481 tatgatccat ctaaccatat cattgttagt atttttctta gtcagttata taaagaatta
    12541 tctacgcaat gtgttaagac taaaggagat aaatgtcacc gcaggattca ttttatttta
    12601 gatgaatttg ggaatatgcc accaatggat aatatggaag gaatcatgac ggtcacagct
    12661 ggccgaaaca tgttgtggga tctgtttatt caatcttatc agcaacttta tgcgaagtat
    12721 ggagaacaaa acgcagcgat tatcaaagaa aattgtcaga cacatattta tattatgtcg
    12781 acagatgata ataccatcga agaattttct aaaaaagtgg ggaataagac ggttgaacaa
    12841 gagaacagta ccatcaattc attaggtatg aatactcata tcaatcgcaa tgttgattca
    12901 gatcgtatct taactcctga aagaatttct acttttttag aaggtgaaac gattgtactc
    12961 gctcctttaa aacgacaaga tttgaaaggt cataaaatac ggccgtatcc tatttttaat
    13021 catggggaaa cgaatcttcc ttatgcttat caatatattg aagatttcaa tccatcgtgg
    13081 gatatcaatg atatagacat acattgtaca catgcggact tggatcttaa ggctttacaa
    13141 attgattatg attatttcct accagatgaa tatgctagaa atctctatca tggactgcat
    13201 gatcaattag aagaaaaaaa cgaagaaaat atgttttata aaatcgttca atctcttgag
    13261 aatgagacgg ctaaaaaatt gttaattcaa ttttacgaag aacaagcagc agatcgactt
    13321 caagaatatg ttgaaaagtt gatcaaaaat aaagaaattt ctgaaatgat tgggaaacgg
    13381 ttgttattgg aatctgaaaa ggtggtgaaa gaatgatcat tggaggaatt ttttctggca
    13441 tagccgactg gtggtcaggc ttattcggag aaggggatgc aaaccccaaa accgtaacga
    13501 aagtcttaca agattatggt aattatctgc agtatcaaga tgtatttagt tggatttgga
    13561 acacttttct gtggggattg attaaaggat tatataatct caatacttta ctagaaaaaa
    13621 ccatttatca aagctttgat ttaaaagact tattgaacgc cgctggggta aatgcactat
    13681 ttcaaagttt gattagtaag gtattggctc ttttttgtgt cgtcacatta atttatttag
    13741 gcttgaaatt ttttatgagt aaacacccac caaaagtcaa aaatatcttg gttaatttat
    13801 ttatggccat gatcttgatt tttggtggga gttctttaat cgatgaaggg ttaaatatca
    13861 gtaaaagctt ttacggagac gtcactgcag caaataaagc agataaaacg ggttcacctg
    13921 cttttcaaat tattcaaaat aatgtgatcg atgtcagtac attactagca actgacccga
    13981 ataaagtaga atccctgcca acagacaaaa gaaatgcttt aacagcgaaa aattttaaaa
    14041 cagcgaatat caatgggatt attactccta aacaagctaa agaaatggcg gataaagata
    14101 agagtggttt atcagattta caaaaggatc ggttaagagc gttacaatac cgattggaac
    14161 tagatgataa tgggaaagaa attcctgttc aaattagtga cgaaggctta gcaaaatatg
    14221 tctatacttc agggtatcgt cgatacagta gtaaaccagg tattatctta gctggtgaat
    14281 cttctttggc ggtggcctat ttatttatcc tatttacgat cgttacttgc attattgagc
    14341 tggtatttaa aaaattctat ttagtgattg ccgcgtcaac ggactttgag acaggccaac
    14401 ggatgaaaag tgcgatagaa gatatttctc aatccttctt gttattggcg tttacgattt
    14461 tagaattacg aatctataca ttaatgcttt caggtatcgg tgatttgcat gcggcaggaa
    14521 agatcaatgg attcttgtat gttgttgctt taattgtgct aactattgcg ctattcaaag
    14581 gctctcaatc ggttacgaag attttcggtg ttgatacgag tctgaaaaac ggcagtaatt
    14641 ctcttcttag catgtttgcc ttaggaaata ttgcgaaaaa tgtgggtagc ggagctaaaa
    14701 accttgcaag tgctggtaag agtggtttaa gccatttaaa tgatattcgt aaaaatggat
    14761 ttggccatcg aaaagatgtt acccctgata acggggaaga acaatcagac gataaagcta
    14821 caactgtacc taatagtaag aaaacagttt ccgaaatgtt cgggaaagcg aaaaatggtg
    14881 tttctaaagc cgcagaaggt cttggttacg tcaatgaacg tgggttaaaa ggaacagctg
    14941 aagatcttac agaaaaagcc aaagacacct tagaagagaa attagacggc acgagagcta
    15001 aagaattggc tgaagctgtg aagaatccat ccgaagcact agaaaaacaa aaagaaaaag
    15061 cttcagaagt caaagaagga ctaaaagatg cgtatggtga aggacaagtt aatgcagcag
    15121 taaaagctaa tacaacaacc gaaaaagaaa atcaagaagg tatagtgatt ccttcagtca
    15181 aagatcacca agaagcgttg cactctaatg aaaattcgat tgaagagaaa aattccatag
    15241 aagaaagaaa ttcagaagaa ggaaaaaatg gacagttacc aaaaactgat ccagaacaat
    15301 atcgctataa acctaacagt gaccttgaaa atgagaagcg aatttcagat ttatcaaaag
    15361 taaatcaaga agacaaagca gaaaatgagg gacagttacc aaaaactgat ccagaacaat
    15421 atcggtatag atctgttaac tccgaaaatg aaaaacgcat ttcagattta ccaaaacgaa
    15481 atcaagaagt acaaacggat gaggattcca tcgttctgcc aaatgataat ggaatcgaag
    15541 ttggaactga aaaatcaacg aaaattggtt tacctaaggc acaaaattta ccaatgaaag
    15601 atagtcgttt gaatcaattc aaggagaatg atcaaagctt tgttcaccaa tcaactgcag
    15661 gtactgttca aatgaaacca agtcgtttgc ctgaaaatgg aaatctgaca actgtttctt
    15721 acaatttatc tgaaagtggt gagacaggga atgcgtccgt tcaaaatgtt gtgacaagag
    15781 aagctgcacc aaaacctatt caaaacgttc atacaggtta caagccagca aatgtggcat
    15841 ttactgcacc aacaaaaata aatgtgacaa gagaaactaa aaacaggaac aatgcattga
    15901 aagaacaact attgagggat atcaaacagg atatcaaacc ataaaaggcc tacaagttag
    15961 gccttttatt tatatgaaaa ggagaaatca attatggata aatatataaa catgaaaaac
    16021 cctccaagat ttagtggaga atggaagttt ttcgcttttt tctttttaga tctattaacg
    16081 cttggtggct gctttttttg ggcgaaaatg gtcaataatc ttgtccactt accactcttt
    16141 tttgcactct tgaattatgg cttatggatt gcgatagcgt tagtgattgt ttggcgaccc
    16201 tataataacc caggtcaacg acaaattttt gcgattatcg agtctatttt ttttgcggac
    16261 aagaaccatt atcatgcgtt agatcctaac cgaaattatc atggtacgta taaggaggat
    16321 gaataaatgc gagtcaaagg acaaaattca gttgaaagga aatccatttt gcagtttgga
    16381 aaaccagaag agcgctctat cttggactta tttccgtata aatcctgtac agaagaggga
    16441 gtcttaatta ctagagaaga tcattttcaa cgttattatc gtgtagtttc tacagacgtg
    16501 gaaggattaa atgaacaaga gaaactagaa cgaatgaatc agttgaccac cgtcatgcgt
    16561 acgtatgtgc caacaattaa gttagttagt ttgaccacag agacggatct aacggaacaa
    16621 atcgtgcaaa agcggcaatt attagataaa aatcgtatgg aacagtcctt aggaagaaat
    16681 ctacaacgat taaaaaaata tgaaaagaaa ttagtcgaag aaatccagga attgaaacga
    16741 gcagaacaag aacgtcctga cttaagcttc tttttcgtca ttgaaggaaa gacaatcaag
    16801 gatttacaaa ccagaaaccg tcagttaatg cgatctagtg gcattttagg gttaaaacca
    16861 ctctacaaaa aagaattgat caagattctg tatcgaatga acaatatgaa tgatgagtag
    16921 gaatggagaa gagagaatga aatatccgtt gatatggtta gaaaaagatg ttccgaagga
    16981 agagaccact agaaaatttc ctcacacttt aagcctgggt aagttgggaa aagggcaatc
    17041 ttttaaccaa gtaaagaatc aaacaagaag aggtgactgt acatgttaga agcaattaaa
    17101 acagcttttt caccacaaaa tgatcaaaaa gtacttgatc cattaaagca actaggaaat
    17161 cgggaaatag aaattttaaa atccaaaggg tatgatttag attttttaaa acaagtgatg
    17221 ccacaaggtg ggttaaattt tgatgaagaa tatgtgacgt atggtaatgg ttattcacga
    17281 tgtgtgacag tttatcacta cacaaaaaat cctacgcttt tttggttggc caatctcatg
    17341 aacaatcctc atacagtggc cacactcgat gttagcacag atgaaaagca aaaagtgatt
    17401 gatcaaatca atcgtgcctt agatgaatta aatgatcgat cagataatga acgaaaagcg
    17461 actgaccgaa ataaaagcgc agaagaatac ctggaactag cggaatatgc tcgtaaattg
    17521 acttctaatg gggaaatttc taagcaagtc aaagtgagag tacatgttta cggaaacacg
    17581 attcaagaag tagatgaacg agcgcaggaa ctgatcgaaa atctaaaagg gatggattat
    17641 cgtgcggtga cttatctgca taaaaccaaa cgagattggc aatcgatgtt taccatgttc
    17701 gatgaacaag ctgaatggtt cggaaatatc aaagggcaat cactaccatc gttaaacatt
    17761 ggcggaggtt tcccgttcca ccaccaaacg ctaaaagatc caggagggac acaatatggg
    17821 gaaaccatga caggcggccc atttgtctgg aatgcgacgt ataaaaatag tgtacggttg
    17881 tctactaata ccttgcttct aggaatgatg ggagctggta aatcgaccgt tcttaaaatg
    17941 atcgcggaag cccatttagc tgcaggagac tttatttggg gctttgaaaa gggaaaagac
    18001 tttattcctt tcttaaagga atataacggt attatggtgc gtttagacgg atctgacgga
    18061 atgatcaatc ctttagagat atttgccaca cggacgtatg atgaagcatc cagtttatat
    18121 gacgatggat cagtcaaaga tttgaagatc aatgaagcag cgtcctatca aacacactta
    18181 gacaaagtgg tctatcaggt acagttagtg agtcctcagc taaaaggaac gatgaaagca
    18241 gaatttaaga cctacttaaa tcgtttttat gaagagtatg gaacagtacc acgcggcttt
    18301 acttcctcaa attcgcgttc aaatacggag acgcaagtca caggtaaaga tccagaagct
    18361 tatccgacgt tcaaagaatt tttagacttt ttaggacaac tggaattacc aggtgcttcc
    18421 caagagaaaa agaatcgaaa agaagaaatg gaatccatcg tagaatcctt atgtgagacg
    18481 tatggcatga tttttgatgg gcatagtacg attcgacacc ttgaccaaca acagttggtc
    18541 ttttttgaca ttgactctat tacaggctta gatcgagggg tacaacaatg tcaaatgtac
    18601 atggctatga cgttgatttg gaatcaggca ttgattcaag gaagaagaca acgacgttta
    18661 attaaagaaa aacaaatgga agaacaagac ttcaaacgct tcttggcgat gattgatgag
    18721 tgccataatg cgattaatcc agattttatt gaaacggtaa attatgtcac aaactttcaa
    18781 cgagaaatgc gtaaagtaga agcaatgact gttttagcta cacaatcccc acaagaaatg
    18841 gtgccagaaa atatgtcttc tgataacttc tcgaaactga agaaagtctt tgaattttct
    18901 tctacgaaga tctttatgcg tatggatgaa tctattttac gtcatataga aggattagtg
    18961 ggtaaatcaa tgacgaactc agaattagaa tccattcctc aacaaaatca aggagacgct
    19021 gtgatcaatt ttggctcaaa agaaaccatt cgtgttcatt ttagtccgaa caaacgtcaa
    19081 ttaaaattgt ttgatggagg aaaataatgg aaaaccaaaa tgaaagttta atcaaacagt
    19141 atgttaagcg tcgtgcaaag cggcgcttat ttttgtgggt gtttggtact tctgcaggtt
    19201 tgattactat tttgatcact gtgtttgtga ccctatttct aatacttgcg gcaggttcga
    19261 ttgataattc ggattcagat tcaagcagtg gtggcgaagc ttttacagga gaatattcgg
    19321 aaggcttacc gatttataaa gaaatcaaag gaagaggccc tttttctgat gagattgcgc
    19381 aatatgctgt aggtgcagca gtgaaatata aattattacc aagtgtgatc ctttcgcaat
    19441 acggttacga atcagcgttt ggtacgtcgg cgtccgcaag aaatgatctc aactattttg
    19501 ggattacgtg gtttgatggg tgtctgtttc ctaaaggaac tgcgcgagga atcggtggta
    19561 ttgaaggtgg ctggtacatg aagtttccca actcaaaagc tgcattttcc tattacggat
    19621 ttatggtagc cacacaatcc aattttaatg cttgtgtcgg aaataaaagc cctggcgcaa
    19681 gtttattgat cttaggacga ggcggttatg ccgctgcagg aattactgaa gatagtccgt
    19741 attatacagg ttgtatgtca attatcactt ctaataaatt aaccgaatat gatgaattcg
    19801 ccatcaaaca ttggggtgaa ggtggcaata acaatggcac aattactggt gaatggacga
    19861 atccattccc tggcagcagt ttagataaaa gttccttcag cggtggtcaa ttatttggaa
    19921 caaatccagg cggtgaattc cgtccgaatg gcttccatga tggattagac tttggttcag
    19981 ttgatcatcc aggaagtgaa attcacgcgg tgcatggagg aaaagtcgtg tacgttggca
    20041 atcctggtat ttcaggttta ggtgcatgtg tgatcgtgat taattacgac ggtttgaata
    20101 tggtatatca agaatttgcg aatagtacag gaaattctcg tgtgaaggtt ggcgatcaag
    20161 tgaaagttgg tcaagtaatc gggatacgag atacagcaca cttgcattta ggtttcactc
    20221 gaatggattg gcgtcaagca caaggacacg cattcacaga tgatggtaca tggattgatc
    20281 cattaccatt tttgaatagc agcaaaaaat agatcaggag aaaaaatatg gaaaataaaa
    20341 ctttaaaaat cgtcttaaca gctgcaatta gcgttagttt aatcggcaat atcgcgttgt
    20401 tttggcagct gaaagaagaa caagagacaa atacctttaa aattgaagaa gtggctaaaa
    20461 acgcgtctga gaagctcaaa aaaaattcct ctgaggcaca ggcgaagatc gacaagttaa
    20521 atcaaaccat caatgagaaa aaccaagaaa tcgaacagct gaagcaacaa gatggcacaa
    20581 aagcaaaaga agaagtaact gaagcacaaa gtaagacggc agaagagttc ggtaaattag
    20641 ccattgataa atcattaagc agaaatgaat taacggaaaa actaaaagat gtcgcaaccc
    20701 aagaagtgat tgacaaatta agtcctgcag acgatgatca ccaacatgat gattacggaa
    20761 gttactcctt tacattaggt gacgtgcaaa cttacgctca aacaagcagt gtaaaagagg
    20821 aacaaaattt tgtcgtattt gttgattata caatcggcaa tcctcaattc aaagatgtca
    20881 aaccacaaaa aataaaaggt ggattaactg tcactgaaaa acaggtagat ggtcaatgga
    20941 aagtcactga ctttagttat ttcactcgtt aggaggaaga aaatgagtaa gaaaaaagga
    21001 gaaagacaac gtttcaaaaa acgaaaaagt tatcttagat ggctgggaat ctttatgatt
    21061 ctcggatttt tgattcttgc tagtccgatt tacacgcatc agaaggtaga cataccagat
    21121 gaagtaagca cgaacgcatt tttaaacacg aaggaactaa cgatggtttc aaaagaaaaa
    21181 ggagcgaacc agcaattagt ttttgaattt gcaatcgacg atgaagatca aagcttatta
    21241 aaagaactgg ctaatcttaa ttatcgcgta gaagtaaaga cagctaaagg ggattatcaa
    21301 gcaataaaag ccaaagtgat taaggtgtct gatgattatt ttgcagtgaa gctatcgcat
    21361 gtaccagaag aatatattgc tatgcgatta accattattc ctgaaaaaat tgatccaaag
    21421 gtggatatgc aagaaccaca agatctgatt tattacgttc acgaagataa ggttaaagat
    21481 caagtgaaag atgaagatta tgaacaacat gcgatcaatt acaaagtgaa aggatacaag
    21541 aaagaacaaa aagcagctca aaaacaaatg gaatctttcc aagcaaccat tgatttgaat
    21601 gaagaattag tgaagaagct aaaagatcaa ttgccttatc aagtcgcaga tgatcaggaa
    21661 aatactgaaa acaagattaa tagctaccag caagaaattg aaacatcgaa aacgcaaatg
    21721 aaagatcaag aagagatcat tcaaaaactt caagaaaaaa ttgatcgtat gacaaaagaa
    21781 aagatttaga aggacaggtg gagtgaaaaa atgaagcgaa caaacttggc tgacaaagat
    21841 actttcgctg agcagaaagt attgattaaa gcctatctca aagttgtaaa agctaaagaa
    21901 atatgtcgag aagtgaattt atctaataca acaatatctg agttgaagaa tggtcgaaag
    21961 aatttagata ctttaaaatt cagatcattt gaagctctat atgtagctgc taaaaaatat
    22021 tttacagctg ataacaaata aaaaacagga gtattttatg tgaaaaaagt atataaaaaa
    22081 tgcttatgaa tcagctatat caccttaacc atttgttaat gagatgggag aaaacattaa
    22141 ccacgattct aacgcaaaca tcaagcacat aaaacattta acactcctcc tttctggctg
    22201 aagatttcaa ccaaacagga agacagcgta tcaattatag catacacttt ttcttaaatt
    22261 catttttcat atgacaaaat atttccttct aatcgacaaa gttagaagga ttttttattg
    22321 tctaaataca caaataacct taattt
//
LOCUS       DBNICI010000030        21765 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.030, whole genome shotgun sequence.
ACCESSION   DBNICI010000030 DBNICI010000000
VERSION     DBNICI010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.030"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..1086
                     /locus_tag="AC1ZCC_000951"
     CDS             <1..1086
                     /locus_tag="AC1ZCC_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-rhamnosidase"
                     /protein_id="HIQ1629219.1"
                     /translation="EEINKRTILALRGQNEIKQHMNTIVDYSILWLIGIENHYMMTKD
                     YEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFIDWSEMDKEGTLAAEQVLLLK
                     AYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNLFDYFWDEELGAFIDCYESGR
                     RNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQITTPYFKFYEQDALCKLGQTN
                     IVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMYGDPYGKSLCHAWGASPIYLL
                     GRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDGKISLEKTKDKIVITSDRTGG
                     TVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(1169..2542)
                     /locus_tag="AC1ZCC_000952"
     CDS             complement(1169..2542)
                     /locus_tag="AC1ZCC_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HIQ1629220.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(2568..2852)
                     /locus_tag="AC1ZCC_000953"
     CDS             complement(2568..2852)
                     /locus_tag="AC1ZCC_000953"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIQ1629221.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(2854..4869)
                     /locus_tag="AC1ZCC_000954"
     CDS             complement(2854..4869)
                     /locus_tag="AC1ZCC_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HIQ1629222.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(5048..5353)
                     /locus_tag="AC1ZCC_000955"
     CDS             complement(5048..5353)
                     /locus_tag="AC1ZCC_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HIQ1629223.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(5442..6473)
                     /locus_tag="AC1ZCC_000956"
     CDS             complement(5442..6473)
                     /locus_tag="AC1ZCC_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIQ1629224.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            6680..7555
                     /locus_tag="AC1ZCC_000957"
     CDS             6680..7555
                     /locus_tag="AC1ZCC_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIQ1629225.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(7707..8549)
                     /locus_tag="AC1ZCC_000958"
     CDS             complement(7707..8549)
                     /locus_tag="AC1ZCC_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HIQ1629226.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(8641..10518)
                     /locus_tag="AC1ZCC_000959"
     CDS             complement(8641..10518)
                     /locus_tag="AC1ZCC_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HIQ1629227.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(10515..11936)
                     /locus_tag="AC1ZCC_000960"
     CDS             complement(10515..11936)
                     /locus_tag="AC1ZCC_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HIQ1629228.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(11939..15328)
                     /locus_tag="AC1ZCC_000961"
     CDS             complement(11939..15328)
                     /locus_tag="AC1ZCC_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HIQ1629229.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            15585..16976
                     /locus_tag="AC1ZCC_000962"
     CDS             15585..16976
                     /locus_tag="AC1ZCC_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1629230.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(17049..17696)
                     /locus_tag="AC1ZCC_000963"
     CDS             complement(17049..17696)
                     /locus_tag="AC1ZCC_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIQ1629231.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(17704..18390)
                     /locus_tag="AC1ZCC_000964"
     CDS             complement(17704..18390)
                     /locus_tag="AC1ZCC_000964"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1629232.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(18396..19469)
                     /locus_tag="AC1ZCC_000965"
     CDS             complement(18396..19469)
                     /locus_tag="AC1ZCC_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1629233.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            19691..20365
                     /locus_tag="AC1ZCC_000966"
     CDS             19691..20365
                     /locus_tag="AC1ZCC_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIQ1629234.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            20370..21710
                     /locus_tag="AC1ZCC_000967"
     CDS             20370..21710
                     /locus_tag="AC1ZCC_000967"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIQ1629235.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
BASE COUNT         6402 a         4089 c         3993 g         7281 t
ORIGIN      
        1 gaggaaatca ataaacgaac gatattagca ttaagaggac aaaatgaaat caagcagcat
       61 atgaatacta tcgttgacta ttcgatcttg tggttgattg gtatcgaaaa ccactatatg
      121 atgacgaaag actatgaatt tttacgcatc gtctacccta agatgcaatc attaatgaga
      181 tacttgatgg agcaaacgaa tgaattaggg tttatttatg ggagagcaaa agattggata
      241 tttattgatt ggtctgaaat ggataaagaa ggaacgcttg ctgcagaaca agtcttatta
      301 ctgaaagcct atcattccat gatcacttgc ggagatatct tagctgaaac atatggagag
      361 aacgaaaatt atcatactca tctctatcaa gaaaaatatc aactattgaa acaaaaccta
      421 tttgactact tttgggatga agaacttggc gcttttatcg attgctatga atctgggaga
      481 agaaatgtga cgcgtcatgc gaatattttc gctgttttat tcgattttgc taacaaagac
      541 caacagcaat cgatcttaaa gaatgtctta ttgaatgaca ctatcacaca gattactact
      601 ccatatttca aattctatga acaagatgct ttatgcaaac tcggacagac gaatatcgtc
      661 taccaaacga ttcttgatta ttggggaggg atgttatctc atgatgcggt aactttctgg
      721 gaagaatacg accctactca aacaggaaat gaaaagtaca gtatgtatgg agatccttat
      781 ggaaaaagct tatgccatgc atggggagca agcccaatct atcttttagg acgctatttt
      841 atcggtttgt atccaacaaa gccaggttat gaagaattta tgatacaacc taatctagat
      901 tcatttgaaa cactgaattg tacgctacct atcaaagatg gaaagatttc gcttgaaaaa
      961 acaaaagata agatcgtgat tacttctgat agaacaggcg gaacagtgaa aatgaatggt
     1021 aaaagttatc ctcttctagc aaaacagcca cttgtcttaa caagctattc aaaaataaat
     1081 gcataaaaca aaaaactgct tgcgacatat cagaagactt taaatcttct tctgatattg
     1141 tccaagcaat ttttattttg aagtttgctt aattctgttc tttagaaaca atgtttgttc
     1201 tttttccggc aaaaagaaag ctagataata cgacaagtaa aaagaaagcg atgattccaa
     1261 ctgtcaaggc tatttgaccg tattctccta gtttcccaag tacaagtcct ggtactagat
     1321 aatcagtatc ggcaaatgta gaagaagcga ttttaagatc tcctaatact ggaaggataa
     1381 ataatggcag ccaactaatc aataagctat tgaaagcaga acctaatacc gcccctcttc
     1441 taccgccagt cgagttgccg aagacaccag aagcagctcc gcagaagaaa tgtcctacta
     1501 ccccaggaat gataactgta gtttgcaagc ctatcatagc aaacatggat agtgttccta
     1561 caaagaagct aacaaaaaag ccaattaata cagcattagg tgcatatgga aaaacgatag
     1621 gaacatctaa tgctggttta gaatcgggta cgagtttttt agcaatccct tggaatgctg
     1681 gaacgatttc tgccaacacc attcgaaccc cttgaaggac gataacaaac ccagctgtga
     1741 atgttccagc ttggatcagt gcaaagatta cagcgttgtc tccattgctt agttctcttt
     1801 caacaaatcc aggaccagct aacaaagcaa gtgcgacata tacgatcgac atcaagagca
     1861 tgatcgatac agtagaatct cgcaaaaagc tgaaactttt aggaatcttt atgtcctctg
     1921 tagaacggtc tttgtttcca aaaaactttc cgatccatcc actagaagca taaccgatat
     1981 ttccggtatg ccctaacgca acttgattgt ttccagtgat gttcttcata aacggttgac
     2041 aaagagcagg cgtaatagtt aatagtgttc cttcgaagac cccgcctaat aagatcatcc
     2101 acaaatttgc agtaaacccc gcgcttacta aaatgactgc tgttagacaa gaaacataaa
     2161 gcattgcttg acctgtaaga aaaatgaatt tatatcttgt gaatctagca agtaaaatat
     2221 tcacgatcat ccctactagc atgatcaaag cagtaggtgt accgtattgt tgcaaagcaa
     2281 tcgccactac tgcttcattg ctaggaacta ctccttgagt atgcagagct gtttggaaca
     2341 tcgaagcaaa aggggttagt gaattactga ttaaaccagc accgccgatc agtactagga
     2401 atccgacaaa tgttttgata gttccttgga tcacttctga aaatttcttc ttctgaagaa
     2461 cgagaccaag cagtgcaatc aaagaaacga ggatggaggg ctgactgacg attgacacaa
     2521 acaaattcaa caatttatcc attgatagtt cctcccttga atcattttta ttcttttgac
     2581 aagatcttcg ttactttttc ttttacttct tcgctgtcaa tgatcgattg caaaggtacg
     2641 agcttttcat caggtataga agttagcgta tctttcaaag aatgctcaat gaagaaataa
     2701 tcagccatat cgctagatac acttccaaca tccgtatgac ttacttcaaa ctcgtccaca
     2761 tctgctccaa gatctgcaag tgcttgtttg atgttcatct ctaccataaa acttgttcct
     2821 aatccacttt gacatactgc aaaaattttc atatcattcg tctccttttc ttatgatcgc
     2881 taatatttct tcttttgttg atgcgtgtaa taaatcttga agattttgtt tattagaaag
     2941 tagctttgtt aaatttgcca aagcccgaag atgtgcctca ttatcgatag cagctaaaca
     3001 gatgaacaaa ctaatgggat gggctggatt atcaagcaga tccacaggtt gttgaagttt
     3061 cagcaaagac atccctactt cgtttacgcc atcttctgga cgagcatgag gtaatgcgat
     3121 gtgatcgcca atatggataa acgcaccata ctgctccacg cgctcgatca tagctttgat
     3181 ataactttct tctattttat tttctgataa tagcggctgt gcggcaaatg tgattgcttc
     3241 ttgccagttt tttggttgat ccgataattg gatcatttct tctgttaata attcagatag
     3301 cataggacgt tgatcctcct tcttttctaa aagtttgttc attttacgat ttaatacttg
     3361 atagagcttt tcttcgtcta cgccttcttt taattcgata taaggcagta gcgccgacaa
     3421 caattcttgg gcagaaggca ttgaaaatcc tggtatcagc caatctcttt gtacttgatt
     3481 gatcagctga tttttttcca agtcagtcat gattggatgc agaacataga gagacttttt
     3541 cttatcagta agtgtgagag gtacagtaga aaaaataata tcgtaagaag tttcgtcaac
     3601 ctcgtgtaag cggtcagctg aattagtctc tgtaaaaaag atcatcggga ataattgctg
     3661 aagttcactt tttaagatca atgaagagct aatgccactt ggacagacga tcacggctct
     3721 tatttttcta tttctttctt ctgcgtccgc ttttctgatc tctccgccaa atagtatagt
     3781 gaaaaaacca atctcttctt cagggatgct cttccctatt cgtctttcta acggaagtaa
     3841 tgcttttctt gtcatttcgt agatggaagc atattgatgc ttaatttgat cgatcatcac
     3901 attaggtaaa taaaaagaat aggaaatacg aaaataggca gggaccaagt gataaaagac
     3961 attcatcaat aattcacgaa acgaagcaaa ttctacggcc atcaggaatt ccatgcgatg
     4021 gatgacttct gaagaacatt ccagcaaaaa atcaagtgca ggctcctgga catctccttc
     4081 aacgatcgat gctaagcaaa gagagaaaaa tataatctcc gaattttgat gttcggaata
     4141 gccaaggcca gtcaatactt gtcgactaat gtcagtgact gcaagagaag ccaacagttc
     4201 agcatctttt accagttcca tggatgtttt tccaattcga atggttgtca aactcaaata
     4261 ataagctaat tcatccattc gatttggtgc gaatcgaaga tctgatagtg aaatcgtttt
     4321 atctagcaaa tctctaactt tggcgtaata atcaatggaa agggttgcgg ttaaatacgt
     4381 gagcccaaat cttccagcaa gtgactgcat caaacgagca gtgcgatttt ttgcttctct
     4441 tcttaatatt gcttctgacc ctagcagcct aaaaccagtt ttcctagtat acgacagctg
     4501 acaatcttcc acttctaatt catttcttat ctttttgata tctgacagga ctgtcccttt
     4561 agaaacctga agaaaatctt gaaaatgaaa aacagaaaca gacgttgcag tcgaataaac
     4621 catcaaataa agaagatctc ttcgctcttg ttcggtatac acagcttcaa atcgagtaat
     4681 atccaaaaat ttttctgccc agtcgattgc tcggaatttc tttatacgga tacgggtatc
     4741 atccacatat tcggcaaaag gagttaattg tttgatcgcc tcgcttgtat ttacttcgat
     4801 gatctctgct gctttttcta gactgatttc tcttggtgtt ttcaggagat aactaatttt
     4861 gtagttcaca gcgctgttca cctcctactt atattctagt atggtagcgc ttttaaatcg
     4921 aggggtgttt ggtacaactt acagttggat tttttttacg actgtaccga acgatttcta
     4981 tcgaatggat acagaaaaaa agcaggtgac caaatgtctc ctgcttttat gaattgattt
     5041 cgtccgatca atgatgatca tgtttatttt ttgaggtcag ttcttcaaat ttttctttga
     5101 catcttcaac cgctttttcg atatcttttt caaagtgatt gggctcatta ggctttgttt
     5161 cctcttctac agaatttgaa ccagataaag ctgtttcttt attttctgtt tcaccagagt
     5221 ttgtgccagc taatgattct tctttgtctt ctgtttcacc agaattagtt cctgctaaag
     5281 ccacttcttc ttctgtttta ggggccattg aacctcttaa tcgtttgtgt ttttcaccgt
     5341 cataatttgt catgttggtt cctcctagta gttctatact ttttcatcgt acaataagac
     5401 agtttatgcg tcaaaaaaca aacccgattc ttacaaatat gtcatatttt tccttctgat
     5461 tcttgaatgc ttttgattcc ttcttccaaa attttcattt gtaagattgt ttcttcatct
     5521 ttgatgactt tttctgcgcc attaactaat gtctcgtaaa tcccttcgta gacacggctg
     5581 taatcaccaa cctcggaaat tactttttct tcatgataac tcccttgttc atctatatag
     5641 gtcaatacac cataatgttc gggcaaatcg ataccaaagt cttcatgatc aggcatgtaa
     5701 aacattttca gatgttcttc ttgtcgatct tttgtttgtt tcacgaacat cccttttttt
     5761 ccgtaaacga cgaaacttgg acgttctttg atgcggaaat aactggattt tatcgaaatt
     5821 ttcatatttc catagaaaaa atccagatcg aagtaatcat tcattcgtcc tttccctaaa
     5881 agttgacgta catcatagct gatatcatct ggttcgccga aataagaaag aacttgatct
     5941 actgtatgac aagcatgtcc atataaatag ctgttcgcag tagaaaattc atgcgtattt
     6001 tctggtactt ccggtctgaa ataatcataa tgcatttcta cttctaaaat atcccctaat
     6061 tttccgcttt cgattacttt ttggactgtc aagaagtcag aatcgaaccg tctgttttgg
     6121 taacattgga taaatagacc tttttctttt gctaatgcaa ataactcttc tgcttccttt
     6181 gacgtttctg caaaaggttt ctctactagt acattcttac catgttcgag tgccattttt
     6241 ccatattcat aatgaaattg actaggtgta gtgatgacaa ctaattggat ctcaggatca
     6301 tcccaaagat cagacagctg atcagtatat tcgatgtctt cgaatgtttt ccacactggt
     6361 tcagatgggg aaaaaatttt tttgatattg attttgttcg taagttttct tgaaaaaggc
     6421 aaatgatagc gatttgtact ctttccattg ccaagataag cgattgtaag catgagtggt
     6481 gcctcctttt attgatgttt tcagtatatc ctagaaaagc gcagaaagta tttataatag
     6541 ctattctggt aaaaaagttc agaaaaacac ttgttatcat tcggtttggt gaaattaatg
     6601 ttcaaagtaa ggataaaaat caaaaagata gtagcggaac caagtgttta tcagtaagat
     6661 agaatttgga ggtcagatca tgcttattga agaaaaacta acaaaacaag agttgtttac
     6721 cacgacagag aaacggattg tcgattacat ccgtaggaat atcgaagctg cagtctacat
     6781 gacgattgaa gaattagcaa aagctacgta tacttcccat tcagctatca ttcgtttatg
     6841 caaaaaaatg ggattcagcg gatttaaaga attccgtttc gagctggggc gggaagttca
     6901 tcaattgatt gctcagttca atcaaacgat tgatcctaat tttccttttt caagtgaaga
     6961 tagcactcag caaattgcaa agaaaatggc ggatttatcg atccagtcaa taaaaaaagc
     7021 acaattgcag attgaacatg aagatttaga tcaaatcggt aaagtattga acaaggcaca
     7081 gcgaatcttt ctttttgcaa aaggtgattc tcaaatcacg gcaaggaaat tccagaataa
     7141 aatggtcaag ttgaataagt ttttgatcat ggctgaagaa tacagtgatt cttcttggaa
     7201 tgcagccaat ttgactagcg aggattgcgc catttttatt agctacagcg gtcgcatcca
     7261 tcattatgaa cgaataatga cctatttgaa gcatgtagag gcccctacct tgctgattac
     7321 cggaaatcag cattcagaaa tggccaaaca ggctgatatg tgtcttgtta tatcgcagga
     7381 ggaatatgat tttgctaaag tagcgacttt ctcctctcaa attgccttcg attacgtatt
     7441 aaatacgtta ttctcagtga tatattctca aaattttgaa gagaatatcc gcaacttgaa
     7501 aaacaaacaa gaattgatgc aaaaaggcct tcttaaagaa tatcggaaaa cttaagaaaa
     7561 gatcctcatt tagtaataag aaagatagtg tttgataggc tctctttgat atcatcataa
     7621 aaacaggctg aagttttctt tttcaaacct cagcctgttt gtttacggaa cgattcgaat
     7681 agatatatta tagaaatgag gaatagctaa cttctgtttc ttattttgaa cgattgacga
     7741 aaaagatata taagaaggag gaaaatcagt atacatgcag ctaacaatcc atacaggaaa
     7801 aatcgtttgt tctcggatac tttctgtact tctttttgaa cttcttttgc atgataagga
     7861 acccggtgcc ctctgacaag taagcgatga ctgttgacca tataaggtgt acaagttagg
     7921 agtgtcacga aatcctgccc tttttcgatt ctcagatctt ttgtatcagt cggttctacc
     7981 gtcttgatct gatcgacttg ataagcgagt gtccgtccat tgatttcgat atagaaagaa
     8041 tcacctaaag tcagtttcgg taagtctgtg aacaactttg cttgaggcag tcctcgatgg
     8101 ctggaaataa cggcatgtgt attttctccc cccgtaggat aagacgttcc ttccaataat
     8161 gaggcacctt tttctagcag cacagtattc gtttcatcaa aaattggcaa atggacattg
     8221 atctcaggga tcgtcaaaat accgatcgta tgtttctcaa aataggttct atcctttttc
     8281 gcaggttctt ttttcttagt aaagggatct gcacctggat ttccaccttc ttttgctaat
     8341 tgctggtttt tctttgtcat gttttcttgg attttagcca tgacttcttc attttctttg
     8401 accgcttgtt gctgataatg agagatgatc tgctgatcca gataattatt cagcgcgtcg
     8461 ctaacaaatg gataagctag tatccctatt cctgagagaa gcaagaaaag catcagcaga
     8521 tctagaagtt tccttttctt tggtttcatt tgtgttctcc ttgtgatgac tgtttgataa
     8581 taaaagaaaa aggagcattt gcgtaatccg ctgctccttt ttccaattct tgcaaaaaga
     8641 ctaaatctga ctgtgcttgc gtctagcaaa gtacagtcca gcaatcagta gaaggactac
     8701 tcctgcaccg atatacacat agattccctt accgcctgtt gaaggaagtg tacctttgtg
     8761 tttatttggt attttttcag gagaaatcaa ctgtcctgct gttacataag attgttcatc
     8821 gatagtaaat gctacacggt ttgttaatgg aacatatttt tctggcgctt tcgtttcttc
     8881 cagatagtac gtgccatatt ttagacctgt cacatcgatt aaaccgttac ttgtggttgt
     8941 aaaaaccgtt gctgattctt tcgccgatac ccagctgacg gcttttgtgg atgggtcgat
     9001 cgataaatat ttcgctgtgt cactatcttg atcacgaacg acgaattctg ctccagcaag
     9061 tgtttggtct gatgtaacgt caccatctac tttaacaaat cgtttgcccc cagtaacgac
     9121 atcgactgac ggtggtgttt gatcatttgt atgcccgtta tcgacatttg cttggttaga
     9181 aaatcctttg gttggatctg ctttttcgtt caaatgcata tagtaaacga atttcaatgt
     9241 accgccagga gttaatgaag gaatataatt cggatcaact gaaaccgtga atccgtctgt
     9301 ttgctcagtg acagaataat ttactgggtc gatttcttta tttccatcat ataaggcata
     9361 agcatacgtt cctgaagaat cattatcaaa tgttaaagca gcgtcatgag tatcgataag
     9421 tttgaatgtt gtgtacttgt tttgcgttcc ttctttatca gcgataccta atgggatatt
     9481 gacagaaatc tcatattgaa ttttttcacc gattgcgaca tcttttccat tcaattgagg
     9541 tgttgtttta tcaactttag acgtatcatt tttgatggtc ttttctactg gtgtttggct
     9601 atttgccagg atctcaaaag gcgtgattgt ttgcttttca atcatttccg cattatcagg
     9661 agcttttact tcttctaaat tatattttcc aacttctaaa tgattaacga tcaactggcc
     9721 tttttcaata gatgcctcgg caaagtcatt gttgccgatg tcataagaat gaccagtaat
     9781 gaaatgtttg gctttggttt gatcagttgt ccaagtgtac aatccatctg tgacactttg
     9841 gatgtatttt ttgacgcttg gtgttccttc ttctttagaa ataataaatt ctgctccatt
     9901 tagtgcttcg ttttcggcag taccgatttt tgtaactttc aacgttccat cattaccgac
     9961 tgtattttta gggtagagat ggatagtatc tagttcttct gtcccgtatt tataagagcc
    10021 atctgcttgt ttgatcatct catatacagg gaaagctaaa accatgtttg cggcagctga
    10081 cactccgtct tttggttctt ctttgatcgt gtagactgca tctttcccat tttgtttttt
    10141 aggtaaagat aaagtgacat ttccatcagc atctgtcgtt cctgaagcaa ctggtgtacc
    10201 aggagtcaaa gactggactg cttgttttgc tgcatccacg gacgctcctt tatctcgttg
    10261 cgcataaaat tcttgagtga cgttataaac tgaaaatgaa atatcggcta atccttggta
    10321 ttgatcgaat tcgctcattt ctttcccgct gttttggatt aaaggatcgg gtaaatcagt
    10381 cattttcttt ttatgaagga tgacttgagc tgctgtctcc tctgcaaaca ctttttttgc
    10441 gccgaagctg tttgtgagta atggtaaaat aaggaaaagg actcctaaca taacgtttat
    10501 ttttttatga tttttcattt tttcactcct gttctttttt tgataaacaa ccagattgca
    10561 aagcttgcga ataccatacc aacaacgcct aaaagtgtat ttttcgtttc gtttgttttt
    10621 ggaagaaatg gacggctatt ttctttttta ttgactactt tcaatatact gtccgcctct
    10681 ttatcctcag aaagaaaagt tgaaatggta aacggaattt ctgtttcact taaaatataa
    10741 ccagaaggtg cttcgatctc ttttaatcga taacttcctg ttttcaaccc ggaaattgaa
    10801 aaagcgccat ttttatcgga aatcaggtga agcgcatctg attcgttctt tgtccacttg
    10861 tatccgttgg ctgtttgctg gaggtaattt cctgctttgt ttttaataag gaatactgcg
    10921 ccagctaatc gtttatctgg tttatttgtc tcgattttga caaattgctt actccctgta
    10981 tatacagaga ctttctttgt actgacacca aaaccaaaat ccagtgttcc gttgttgttg
    11041 atttccttgt tcgcctgtgc tgtactgctt agctgcatct gataagaaat ggtcagtttt
    11101 tttccagcat atttttccaa cgaagggatc gaaaataaaa catcaaatcc gtgaggtttc
    11161 ttgtgtatga caaagccttc tgtcagcttt tctccattcg atgaaatcgt taaactatta
    11221 ggtaaaaacg ttaatgcttc atccgcacta tctgacaatt cgaacttttg atagtcaagg
    11281 atatttacag gaattgtcgt atgcaaggaa taagtgattt tttcaccaat cgtaaaatca
    11341 tttctaggct cttctaatac cttctcaaaa ggtggatcag ggtagtcgtt ttcttcattt
    11401 ttaggataca aatggatagt tgaaagtttt tgattgtttt gtccatgtac aggaagaaca
    11461 actaccatat tctctgcctt ttctttgacg acttctggta ctttggattc aatgaaaaga
    11521 tacgctttat ctcttttttc ttgatcttta gcggccagag aaaaagaagc gataccatct
    11581 tcgttgttaa gagttgtagt tgttgcttct gcggtaaaca taccggaaga cgcaccgttt
    11641 tttgcgatct ctgcttgtgc ttcttctacc gttttgccct tttcccgtag atggtaaaaa
    11701 gaatctgtga catcataaac ttggaatgtg acaccattta atcctcgata cgtttgtaat
    11761 aaagcttttt cttgtccgtc atttggatga tttttcggca gttgcccgtt tgggaaaagc
    11821 aatttgtgta aagtaatctg aaccatttct gtttctgttt ctgttgctac ctgagtaaaa
    11881 gctggtttaa atagtaacaa gaagagacac atactaagcc aaccaagttt tttcatattt
    11941 agctgccccc tctttctttt ttcaaaaaaa gataccaaat agaaaacgca caaccaatca
    12001 tccctactag atagattcct aaacggccaa ttcctcccgt ttcaggtaat ggtacttttg
    12061 cctgattcgt gatgtctaaa gaaatctggt tgtttttggc tcctggtgac agaacagatt
    12121 catgatcttg tccatccact tgaattgacc catcttcgtg tataactata gtaactggct
    12181 cttttagacc ttggtatcct tctggtgtaa aagtttcggt cagcgtataa gttccaggtt
    12241 ttaaattttc gaatagaaag gtatctgttt cttgtccatc ttttggcgat tctaagtcca
    12301 tttctggtcc ctgcagtctg aattttgctc cttttaatgt ctgtccgttg tcttcttttt
    12361 tgtgtaccgt tagatcaaac gcttttagtc gattttgatg agtgagcgtc catacattat
    12421 ttgtcatctc ggtatttttt cccgtgtaaa tgatttttcc atatttatcg atttggaatt
    12481 cataatttcc cggcgaagta tcatatccgg caggaccagc tgtttcaacg actcgatatt
    12541 taccaggtgt gagtgaatca aaactggcaa gacctttttc attcgttgtt tggctgtcaa
    12601 ttggctgata agtaccattt gcttcttttt tctgtaggga aaatgctgct cctttaagat
    12661 ttatctcttt gtccgtccct tgcatcgcat acttacgtac tgctacagga acttcaacaa
    12721 aaggattttc aatagtcaac tggatcgtat catcggaagt agtgactgtt tttccatcaa
    12781 tggttaccgt accatcagaa gcgatcttga tttcccaagt agtctttttg cttaatccat
    12841 gcccttctgg agcttttgtt tctgtcagcg tataggtcat ttctttttgc aatttgacat
    12901 tttctggaag agaataggtt ccgtcgccat gatctgttag taaagtatca atagaatctc
    12961 ctgttaattt gaaaacagcg ccaataagat ccttttcacc tgtagaggaa tttttcgtta
    13021 ttttcaagtt taacggtgtg aattgtttag tatttttcca tgtcattgca tctatttgtt
    13081 gagaatcgta tcctggtaca ggtaattctt tgattgtttg gtaactgaat gcttgacctt
    13141 gattattgta ttgaggtagt gataatatct cttgataact ttctccgcta tttgcagata
    13201 atttgtcaat gtctgcacgt tcccacgtat ttgttgtatc tttagctggt ttaatgattc
    13261 gaatatatcc gtttttccaa gctgcagcat ttgtcgtatg ttcccgttga atctcaaaag
    13321 taacttgatc tggacgatca gctagattgt tgtcaaattc ttcccataac tttttgatgt
    13381 gaagactgac tccgggagct ttagcagatg gtataccgaa ttcagctaat tcattggtat
    13441 caatgtttgg ctggaaagtt gtcctgccgt tcatttgata ccagaaattt ggatggaagt
    13501 cctcattttc tgtttggatt ctcacttggt aatggatttg gatttcttgg tcttttccta
    13561 aatagatctg attgctcttg atggtatttc cttctatgga aatagatgga gtgaccgttg
    13621 taggacttgt ccccacactc ttgactgaaa gtgtaccagg ctgataaatg aatggttctg
    13681 cgattggatc atttatttgt ccattgctta cagttgctga gatctgtaca gcttttttgg
    13741 ctaggtattc agagatatct gttgcatgat cagctgattc atagtaaaga tcgccttttt
    13801 catctgatga aaccatttgt cgcatacgag actctacttc tgcttttgag agaccagctg
    13861 ccggatcgct ttgaagttgg atgccaagac catgtatttc gattcctcgt tctttgagtg
    13921 ccatcgcttc tccgatggtt gcgataaacg tactgtcgat tcttctggat agattgtttt
    13981 ggtcaggtgc atcatagatt cttgagatta gagaagtatt tcccggccga tcttgcgtat
    14041 tagaaaactg agttccgtaa taattgctgc ttgattgtgc gtgtacccgc tgtactttat
    14101 aggaaaatgt tggtacacca tccgtcagca aaacgatcac ttttttatgt ccatttggaa
    14161 cggatagcat gcttcctgca tctcttagtg ctttttgtgt aaaagtacca ccatttgtcc
    14221 gtgaaggtgt aatggatttt acttgatttt tcactgaatc aaatgaaccc atctgtactg
    14281 caccgttact gtagctatat ccttcgcttg agtagccgac atatcccata ttgattttgt
    14341 ctgtgatacc gctatctgct aaagtatcga caaaacgatc gacaccaatc tttacttcac
    14401 cgatccgatt attgtcgttc atacttcctg accagtctac gaccaatacg agatcaagag
    14461 gcgtgatatc tttttgtaca tttccacgag cattcaaata aacatcaaac aatccgggtg
    14521 tacttgtttc ctttgcatac tttcgtatcg catagtctgc ttgattaccg gttcctccat
    14581 attcgatata cgaatgggtc cgatcattag gatctccatt ccaagaattt ataccatccc
    14641 attgatttgt tccgttttta ttgccttgat gatcaagaac atttgtattt cctgtaggtt
    14701 gccaactggc tttaggataa gtgccctgat catctgttgt atattctgta ggaatcgttt
    14761 gattagatgg cacactcaca gctggcaagg ataatgtagg caatgtaaat gggctgacag
    14821 aattttcgtt ttcttttgat gattcttctt ctatttcgtc tggctttggt actgacacat
    14881 ctgtatcttt cttattttct gtctcatcaa taatgtcata ttctttatta tcgacgatca
    14941 gcttgtttgc ctggctgtgg taaagtgctg gatcgattgg aaaactcaga tcgatagttt
    15001 gtttagtgtt tgatgagatt tccaacgtaa ttttcccgct atttacctga taagcatagc
    15061 catctccttc ttttctttcc acatcactta agtaattttc gactagacca atttcttttt
    15121 ctatcttttc agcactggtg ttattcgcat ttagcttgat gttcagtgct ttttttccag
    15181 actgatccga aatgacttca gctgaaattg tttctaaaac tgttggatca tctgtagtgt
    15241 ctgctattgc agataatggc acaaagtttg ataaactcaa tattatcagc atgaaaataa
    15301 ctttcagttt cttccctgtg gtcgtcattt ttcccctcct ttttcttttt ttctgttttg
    15361 acgtgaatag tgaaaattat taaaataaac aggcgttaca taattttatt tattttaata
    15421 tgataattat tatacgaagg gcgctaaagg gaatcacacc aattttcata ccaattggtt
    15481 tcatttatga agttgattcc ttcgattaat gagcgtttca ttgaaagaaa agggttgagg
    15541 gagaaaaaca tatgatcagg ttgttggaat taaactatag aaagatggaa aaaatcattc
    15601 gaattctgtc catttctgac aagtggatga ctaaaaaaga acttgccaaa cgaattggta
    15661 gttcagaatc tacatttgta aggtatatcg aagagatcaa aatccgctgg ggaacggatg
    15721 taacgattca atcttcacaa aaattggggt atcgtcttga acatttgaat gtaccagctt
    15781 atttaaatgt tctgatggaa atgtcgcaaa gttctacaaa cagtcgttta ttaaatgaga
    15841 ttattgtcaa tccgggtaaa acgattgagt attattgtga aaaaatttcc atcagccggt
    15901 cttcttttac acgtaagttg aaacaatgca atcaaatttt agaaagctat ttattaaaag
    15961 tgatcgttga ccaaggtttt cagttaacta gccttaaaag tgagctccag ttgcgtatct
    16021 ttgttacctt tttcttttta agttactacg gaagacgcac gttgccattt ccaatcgaaa
    16081 aaaagaagat caaagaaatt atggtcaaga ataattgtca gatgaatttg tttcaggaag
    16141 aaagcccata tgagcaatct ttctttatta tgtattttgt tgtgcatctc atgagagaat
    16201 cccaaggaac tccatttgaa tggaaagctg gagactctta tgtacagttg ataaatatcg
    16261 ataaaaaaga cttcttaatc ttgaaaaatc tcttaggaaa attgaagtac agcagctata
    16321 taaaagtagt gaactatttt gtcaatacga ttttttcaga attttcggag aaagaaaaaa
    16381 atgaaatcag tgagcagata cgaaaaaata tgtcctactg ttcgttttca tccgctgatt
    16441 gcaaagaaaa aaacgaagaa tatgttgttc aattattaac gcaaatttat tttttctccc
    16501 gtattttccc atacgatacg actcatttga cgatccgtgc aacttacttt gcacagcaga
    16561 tgcaacaccg ccagatacgt ctatatgctg gatttcgtgc agaacttcaa ttttattcca
    16621 ctctactgaa tcagaatctg atttattatt tatctagcta tatcttttgg atgcttatcg
    16681 gtaatcctga aattcaccat tatacttctg ataaaaaaat actgattatc agcgacttaa
    16741 gtcttcgaca tagtcaatat attgaagaat atatcagtga tattcttgct gtgcataaaa
    16801 ttcactctga gacaactgct attactgaag atcagctgtc gaaatacaat cttctcgagt
    16861 atgatctgat cgttacgaat caacccattt tgaatgccca tgttcctcat atattgattg
    16921 atgatagtgt gtcattcgca aatgaagaag aactcatccg cttattcgag ttatgaataa
    16981 tcaaaaaaca gctatgtata aaccaccctg ataaaaattt caggacaaat acacatagct
    17041 gtttcttttt attgtattcc ttcagcaatc caacgagatt tatagcgaaa gtcttttaac
    17101 aactcttctt ttgtccaatt atttgcaatg agatccgtca aagtgttcat tacgagggtc
    17161 atggaaaagt ataggaaact aactgcttct tccgacgatt gaatggagaa atgattgatt
    17221 cttaataatt ccagccattt ttcgttcatt ggagaatcag ccgatagttc tctacgtttg
    17281 gttgtctgct gatcatttcg ctgggttaac aagtataacc tttgccaata gatactttcg
    17341 tgatctaagt cgcagcacca ctctaaatat ttttcgatcc ccaaaaagaa atgttgtttg
    17401 ttttcgctaa tgaattttaa gatatcttga tggactatgt gagaactgct tttgatcgta
    17461 tacaaataag catcttccaa atcttgaaaa tatttataaa aagcaccgcg cgacatcccc
    17521 atcttgtcaa cgatttctga gactttgact tggctgatgt gctgtgagca aaatatctcc
    17581 agtaataggt catcgattct ctgtttcttt gcgttcgata aattgaaaaa agtttctttt
    17641 ggcatccaca tctctccttt atctctgatt atacgtggat agcccttcat tgtcatgaag
    17701 gattcattcc ttctttttct cttcttctgt caagtaccca tcttccattc gataaacgcg
    17761 atcactccat tgaatcatac ggacatcatg agtaaccata atagtcgctt tttgtttctc
    17821 atgagcttct ttaactagta atttgacgac ttcatatgca tgatctgtat ccaaactagc
    17881 tgttggttcg tctgctaaga tcaagcttgg ctcattatat aaagcgcgag caattgccac
    17941 tctctgtctt tctccaccag aaaggtcttt aggataacta tttttaagtg actggatatc
    18001 taaagattgc agcaaggaat cgatttttgt tgaatcttct ttttttgaag taacttggtc
    18061 aactagttca aattgttcct ttaccgttaa aaaaggaatc aaattagagc tttggagaat
    18121 aaatcctatt tctttaaagc gtaaatctgc tcgttttttt tctggaagct cagttagtga
    18181 gtgaccgttg atcactattt ctccggaagt cggtgtttgc aatcctcctg aaatggtaag
    18241 gaaagtactt ttacctgaac cagaaggacc aatgatgctg acgaattctc cttcctttac
    18301 ataaaaatca attgctttca aagctgagac ctttgtatga ccagaaccaa atgttttggt
    18361 aacttttttc atcgttaata tttttttcat ttttattaac ctcctattgc tgtaattggg
    18421 tctactttgg ttatcgtacg tatcgaaaac aatccgccta gcattgcgac aatgatcaaa
    18481 atcccactat atagcaacca ttgtgaccaa aaaacagcaa aaggcatcgc gcttggtaaa
    18541 atcagactag tcaaataagc aaacaaaaga gcaagcagaa cgcctaccac cccaacaata
    18601 aaggactgtg ctactaaaga tttagcgatg aagaaatttt tgatcccttg tgctttcatt
    18661 actccaaaaa tagcagtctt ctgaagtgtc atcacgtaca tgaaaatacc gaccacggca
    18721 gcaaccacta agaacaaaaa gtatatcatc gcatttaagg tcatattttg ggcagaatag
    18781 ccaggaatac tttcaatgaa tgtcccaata gttaataact gtagaccctt cgatgctttt
    18841 tctttcgaaa ttttagctga atcctttatt acgatcccat tgatcggctg ttcttcttca
    18901 gaagcaaacg gctgttgtcc atacttcaaa gcagtccacg tatctaggga cgcatagatg
    18961 acgggagaaa ctgtgtaata agtttcagga aagataccga caatcgtcaa aggatcgtcg
    19021 taactgccaa ttttgatcgt atctccaatt ttatagcctg aatcagccag attttgagag
    19081 atgatgattt cattctttgt tttaaattca tgccctttcg tgagttttgg taataaaaat
    19141 gcttctttcg ttgtgccgaa aactgtaaca ttttctttgt tttttccttt gattgctccg
    19201 ctgtacaatc cgattggtgc tttctctcct ccttcaatgt atttcagatc tcctcttgtt
    19261 aattgtgaag ctgcaaatac ttgatttgca tcctccgaca aaacaatctc ttgtgcgtcc
    19321 caatcatcga ttgctttttt aaactcttct gaaagtccat tcgctagtcc tgacaacatg
    19381 aatacgacat acgcaatcaa gaacataata ccgacgatca agccatatcg taatttagaa
    19441 tagcgaattt ctttgatagc tagaaacatt tgtttttcct ccctatgata gattgatagt
    19501 gacaaaatgt cacttgttga atagtgtaac tctaaagtga caatttgtca cttgtgttgt
    19561 ttttttctta tcaataaatt gtctaagaca tctttcgata aaaccttaac atgagtcata
    19621 aaaaatttat ttaagcctta tttatgcttt ccatgataaa ctaaagtgac caaatcaatg
    19681 aggtgggaaa atgattaaac tattagtagt agaagacgat aaagtattat ccaataatat
    19741 aaaagaaata ctcgctgcaa tcggcgagat aacacaagtt tatgatggta gtgaaggact
    19801 gttcgagttg gaaagtggag tttatgatct tgcaatcttg gatttgatga tgccggaaat
    19861 gaatggatat gaagtgttag caaaaatacg gaaagaaaaa atacagactc cagtattgat
    19921 tttgactgca aaagatagtt tggaagataa gattgaagga tttgaacgag gtgcagatga
    19981 ttatctaaca aaacctttct atcgagaaga actgatcatg cgcgtgaaag cattattgaa
    20041 acgctcgcta ggtctctttg atgaaaatgt tctgaagtat aaggatatca gttgtgattt
    20101 aaaaagcaat gaagtcttgt ataaagaaca agttttgccc atacaaggaa aagagtttga
    20161 attgctcgtt tattttttac aaaataaggg aatcattttg acaaaagagc agatatttga
    20221 ccggatctgg ggatttgatt ctgatacgac gattacagtt attgaagttt atatgagcaa
    20281 cttacgaaaa catctgcgaa gtactggatt ggataaagaa atcaaaacct tacgtaatgt
    20341 cggttatata ctgcaaaggg tgtgatgcaa tgaatggaga tattacacga aagcaacgta
    20401 gtcgcttttt tgcgacaaat gtagcagcat ttgcttttat cttttttctt ttgggattca
    20461 ttactttaca gatattaaac caatctgcat acagagaaac agatctgtca ttagagaaaa
    20521 taaattttga ttctcgaaca gtacaattag agatacaaag atatcagcaa ggcgatccct
    20581 ttttaaaccc gactcaaacg gaacaaaaca gaacaaacat ggaacagcca ttaatggaac
    20641 catcaaatga ccgttttaac actcaagtga tcttgtggtc taaaaatggt gagatattga
    20701 ataaagcagc tattggtggg cgtttcaacc aaatcaaaga attaaaaatg gatacgaaaa
    20761 atctgaatac catccaaaat attgaattaa atgaagacag tggctcatct gccctctctt
    20821 tccgttcgat tacgaaaaaa gcggagacga atcaagatgg cgttgcctac atccaaattt
    20881 tagaaaatac aaatcaaatc aagaattcat tagcgacttt tcgcacgatt atgattttgt
    20941 gtatgattgt tttttggatt ctctctattg gtatcagtta ttacttgtct agtctaagca
    21001 tgcgccctat tattctttct tggagaagac aaaaagaatt tgttgagaat gcttctcatg
    21061 aattgcgcac accgttaacg attattcaaa atagtttgga acatcttttt accaagccta
    21121 atcatactat tattgaagaa tccgaaagca tcgctcaagc attgtcagag acgaggcgcc
    21181 tcactggatt gacttcggat ttgctgacaa tcgctcggaa tgattctgat cagcagctgc
    21241 tttcaaagca aatgatcaat acacaagaat atatcgaaca acttgttaaa ccatttcaag
    21301 agatggccat catagatgga aaagagttta ttctccataa cgatgcaaca ttagaagtac
    21361 caattgatga aaaaaaaatc catcaagttt tggttatttt gctggataat gcattaaaat
    21421 atacgaatcc agaagataag atcattttgg attcatctgt taaccataaa gaatggctga
    21481 ttcacgtaaa gaatactggc ccttctatca gtgaagaaga tcaagcaagg atttttgatc
    21541 gattttatcg ggaagatcaa tctcgggcga aagagactgg cggatacggg cttggcttgg
    21601 caattgcaaa acaggtaatc gaacagcatc atggacagat ttctgtatcc aattatcttc
    21661 cacaaggagt cgattttaaa gtccaattgc cgttgaagcc taaaaaataa tgcttatccc
    21721 taaaaaaggg ctgtgacaaa aatcttgtca cagccctttt ttgtt
//
LOCUS       DBNICI010000031        21371 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.031, whole genome shotgun sequence.
ACCESSION   DBNICI010000031 DBNICI010000000
VERSION     DBNICI010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.031"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(73..143)
                     /locus_tag="AC1ZCC_000968"
     tRNA            complement(73..143)
                     /locus_tag="AC1ZCC_000968"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(109..111),aa:Gly,seq:ccc)
     gene            260..1012
                     /locus_tag="AC1ZCC_000969"
     CDS             260..1012
                     /locus_tag="AC1ZCC_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HIQ1629236.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            1028..1507
                     /locus_tag="AC1ZCC_000970"
     CDS             1028..1507
                     /locus_tag="AC1ZCC_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HIQ1629237.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(1758..3338)
                     /locus_tag="AC1ZCC_000971"
     CDS             complement(1758..3338)
                     /locus_tag="AC1ZCC_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HIQ1629238.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(3331..4074)
                     /locus_tag="AC1ZCC_000972"
     CDS             complement(3331..4074)
                     /locus_tag="AC1ZCC_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1629239.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(4250..5026)
                     /locus_tag="AC1ZCC_000973"
     CDS             complement(4250..5026)
                     /locus_tag="AC1ZCC_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIQ1629240.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(5274..6110)
                     /locus_tag="AC1ZCC_000974"
     CDS             complement(5274..6110)
                     /locus_tag="AC1ZCC_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family putative permease subunit"
                     /protein_id="HIQ1629241.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(6121..7050)
                     /locus_tag="AC1ZCC_000975"
     CDS             complement(6121..7050)
                     /locus_tag="AC1ZCC_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HIQ1629242.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            7242..7595
                     /locus_tag="AC1ZCC_000976"
     CDS             7242..7595
                     /locus_tag="AC1ZCC_000976"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIQ1629243.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(7669..8325)
                     /locus_tag="AC1ZCC_000977"
     CDS             complement(7669..8325)
                     /locus_tag="AC1ZCC_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HIQ1629244.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            8551..10485
                     /locus_tag="AC1ZCC_000978"
     CDS             8551..10485
                     /locus_tag="AC1ZCC_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIQ1629245.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(10571..11794)
                     /locus_tag="AC1ZCC_000979"
     CDS             complement(10571..11794)
                     /locus_tag="AC1ZCC_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HIQ1629246.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(11788..13659)
                     /locus_tag="AC1ZCC_000980"
     CDS             complement(11788..13659)
                     /locus_tag="AC1ZCC_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HIQ1629247.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(13680..14798)
                     /locus_tag="AC1ZCC_000981"
     CDS             complement(13680..14798)
                     /locus_tag="AC1ZCC_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HIQ1629248.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(14881..15714)
                     /locus_tag="AC1ZCC_000982"
     CDS             complement(14881..15714)
                     /locus_tag="AC1ZCC_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1629249.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(15707..16624)
                     /locus_tag="AC1ZCC_000983"
     CDS             complement(15707..16624)
                     /locus_tag="AC1ZCC_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1629250.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(16851..17975)
                     /locus_tag="AC1ZCC_000984"
     CDS             complement(16851..17975)
                     /locus_tag="AC1ZCC_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIQ1629251.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            18158..19066
                     /locus_tag="AC1ZCC_000985"
     CDS             18158..19066
                     /locus_tag="AC1ZCC_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIQ1629252.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            19207..20820
                     /locus_tag="AC1ZCC_000986"
     CDS             19207..20820
                     /locus_tag="AC1ZCC_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIQ1629253.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         6751 a         4126 c         3588 g         6906 t
ORIGIN      
        1 aacataagaa gttgtttttg cttggctgtt cttattactt gttgcagagc agctttttca
       61 caacctattt taagcgggtg acgggaatcg aacccgcgac atcagcttgg gaagctggga
      121 ttttaccact aaactacacc cgcaatacaa aaacagtata cgcctctttt attccttcct
      181 caagtttttt tattctttac gcttctggta tgttttgtag taaaataaaa ctaatcaatc
      241 aattattgga gaagatgcta tgacgggaaa agaattagat ctgttgctgg acacttgttt
      301 actcgctggg aaaatcatga tggaaagcaa tgcggagatg tatcgggtag aagatacgat
      361 gagtcgaatt gctttagctt ctggaaattt ccgtttggtc agctatgtca ctcaaaccgg
      421 tctctttatt ggtcttgatc gtacctcgac catccgaatg gaacaaatta ccaaccgttc
      481 gattaattta gaaaaagtag taaaagtaaa tgatctttca cgtaaatatg ttgcttcaga
      541 gctaacatta gaagaacttt actgcgaact gcaagcaatc gaccgagacc gttctttctt
      601 tcctgtctgg ttgcaaatcg ttagcgcggc agtgatcagt ggaacaatta tggtgttatt
      661 tggcggaaag atgaccgatt tacctgtgac tttagtcatt ggaggattgg gatacgcagc
      721 ttatctatat agtttgaaat tttttagaat caaattttta tcggaatttt tatcttcttt
      781 attgattggc atagccgcta tctacagcgt acgttttggc ttaggaagca atcaggatct
      841 aatcattatc ggatgtgtga tgcctttagt tcctggagtt caaattacga acgctatccg
      901 tgatttgcta gctggacatt atgtctcagg agtttctcgc ggaacagaag cgatgatgac
      961 tgctacgatg atcggttttg cgattgcttt tattttccag cttttttatt aggaaaggaa
     1021 cacaagtatg gggacgatat ttattcagtt ttcttttagt tttttagcga ctgcggcatt
     1081 tgccgttatt acaaatgtac ctaggcgttc attgatttgt tgtggtcttt ctggtgcttt
     1141 tggctggatg atctactgga tctgtgttca aataggcgga tctgctgctt ttggttcatt
     1201 gcttggttct ttaggagtag cattcatcag cgatcttttc tctaaacgtt tgaaaatgcc
     1261 cgtaacgatt ttcaatattc ccgggatggt tcctctagtc cctggtggtt tagcttatca
     1321 agcggttcga aatctcgtta ctgaatccta tcgagaagcg gctcattatg cggtccaagc
     1381 aattatgatt gctggagcaa tcgctttagg tcttgtatta tcagaagtct ttaaccataa
     1441 catccgtaat ttcaaagtaa agcgtgacgc aatcatgatg aaacgaaaaa aacggctttt
     1501 taaataaacg aagtgtaaca aagaaaaggt tgtgaaagag acgttcagcc ttgagcgatg
     1561 acaaaatatc tcacaaacat tgcgacaagt aaccgcagga gcaataagaa gtaattatga
     1621 gtattaaaaa aagttttgta aaaatagctt ctcacatttt tacataaaat tgacttaata
     1681 ctgagaaact gacttttgaa caccctctat ctgtaaaaaa ggggctgcga catgattttt
     1741 gtcacggccc ctcttcttta ttcaaatttt ttccaaaacg ttcgttgata ataaacgatc
     1801 catagagcgg aaagtattag aactaaaccg atcacaggaa tattcactaa ccaaaatgga
     1861 taaaacaaag ccaacattcc gtataagacg ataagaataa tactgatgat caatgaaccc
     1921 atcatagttg cgactaaacc caaacttcct gaacctctgt taaataattg gttaatattg
     1981 gtccagttca gcagcagcaa ccgataatct ctagcgaaat aacgtagtga caataagtag
     2041 cagccgaata aagaaccaaa tagcaagcta gggataaaaa acaaaggcag ctgaaaagac
     2101 agtccgctta ataatgcgat cccaccagtt aaaaaactct gcaatatcca tccgaatctg
     2161 aacttttctt ttaaatattg gttcatagag attggcaaag aacggataaa cagaaaattt
     2221 tcttgatcca aagaaataag attgctgata aaagaagtct gattaactgt taagaaagcc
     2281 aatgcgatcc ctaccaggaa aacaacaccg attaacttat tatctaaatc agttaatttt
     2341 atttcgcctc ctagggcaaa agcaagaata aaaatgattg gagttaacaa ggaagaagaa
     2401 agaacttgca taatcaaatt aggttctttt actaattgag cattgtaact aaataaaagc
     2461 tgatgcacat tttggttctt tttatgggtt ctttttattt tgaactcact gtttgaatca
     2521 gtcagttgct ctgtaagctt tggtactatc agctgtttta cagcaaggaa aaatcccaga
     2581 gccgtgatag ctagtattcc ccaacccaat aaaccttgtt gagaaagtgg ctgactggca
     2641 gcatcataga atggcaataa tatcgaaatc ggatgccgat ctgttaactc attttcaata
     2701 gtccccgttt gatgattcat ccacatgatt ccagcaaatg cgatcagcat cgagctgcca
     2761 agcaagatac tagaaactgt ttttctatgt tttttaaaca ctgctgttcg agccaaacca
     2821 aaaacaatca aactagccag acaaaaaaga ataatcaaaa atagagaaaa caaaaggatt
     2881 gagataatga tagtaagcaa aatccctaga tcagctcgcc atccggttaa aataaacatg
     2941 actagaagcg gaaaaacaaa aggaatgatg gtcaaagcaa ctaccaaaat ctttgctgaa
     3001 aaaacctgtt cttgtccaaa aggcagaggc aaaaatactt gcaggtcatt tccttcaaaa
     3061 aagatattat aaatgactga tatactttga gttaaaccta acataccaaa aagtgccacg
     3121 taataagtaa agaaacctgg caatttaaca aagttcatag caaacatagc aaatccgtaa
     3181 atcagtaaaa aaatcagccc tgaaaaaaga ttttgagaca aaagatagat agttaatcgc
     3241 tttccctttt ttcctttctc tcttgcttta ttcgtgagct gtggattaac atataaaaga
     3301 ttaactcgaa ttaattctaa taattgtcgc ttatacatac ggtccatctc ctgtcgcttg
     3361 ccttccagcc attttcaaat aaatcttttc tagcgtttca tcaggtgagc cattcaataa
     3421 ttcactaact gccccctgat aaatcaattc acctttcttc aaaatagcca gtttatcgca
     3481 taactgttgt gcggtatcca atacatgtgt agaaaaaatg actgtttttc cttttgcggc
     3541 atgagccttc atcatttctt tcaaatcaaa tgctgcttga ggatcaagtc cttgcaaagg
     3601 ttcatccaat acccaaatat ctggatctgg cagcaacgtg ccgatcaaaa tcgttttttg
     3661 acgcattcca tgtgaaaaac ttgcgattgt ttcattttga tgactataca tatcaaaaag
     3721 cgctgtcaat tctgctaaac gcttttcttt tttttgtgtg tctaattcat aggcagctga
     3781 aatcaagtcc caatattctc cagccgtcaa ttgtaaaaaa atatctgggg aatcaggcac
     3841 ataaccgatt ttttgtttga tactcaatcg attttccgtc aatttcattc catctactaa
     3901 aatcgttccc gatgatggtt caataatact aactaaactt ttaatcgtag ttgattttcc
     3961 ggcaccatta tgtcctaaaa atccaaaaat ttccccttgt cttatcgaga gagacaagtc
     4021 ttttaatgct tgttttttcc cgtaactttt acttacattg atgaattcaa tcatttctgt
     4081 actccttttg tttgttatta ctttttattg tacccttcgt tattggtgga gtaaatgtca
     4141 aaaagagtgc ataacaaaaa aggatagaaa cgactaggct gctacttggg ttgttctctt
     4201 ctacaggaaa acactcttct aacaggtaag atatgagtct ttgttttctt taaaatgatt
     4261 cattttctat cttttcttgc ataaaattct ttagtttatc aatacttctc tttcccatct
     4321 ctagttgttc tccatttgca aatgttacta tttcgtttac tctgctaata gatgtaatgt
     4381 tttcagtatt aatgatataa gcttttaaat ctttacataa atactcatat tcgagtgatt
     4441 ttttatattt tttaattgtg ccgtttacga gaatctcttc atctttcaat actaggccta
     4501 ttttattttt ataacctggc atgctcatta gataaaggat ttcatcgtaa tggatatagt
     4561 gatcgctatt accataacgg acagttgtcc accttttttt cgtagcatgt tgaacaggat
     4621 agttttcttt ttctttcaat aagtaacttt ggatgctttt tttatttttt tcataagtga
     4681 attcttttat caaataacca ctaggcgtaa tctttcgatt aataacagtt attgctttat
     4741 tcggatgaga cgttagataa acaattttac acttttcatt cttttctcta agcatttcgc
     4801 ctagttcgat accgctataa taggcgttca aatcgatgtc taggaaaaaa atatctgagg
     4861 aatagatttc attattttta atgaattcaa caaattgtat aacgttcaat ccctgaacga
     4921 tctcaaattt ccaaggactt aataaaattc tggattcttc caaaatttct actagcattt
     4981 tttcaaaaac caaactatca tccaaaataa atatacgcgt taccataaaa agtacctcca
     5041 ctcgatattt tactatcaaa atcatttcat acaacttttc aacatacatg ttaggaataa
     5101 attgtaattc tcttcacaat cttttccaac acctttgaaa ccgatatcaa atactttcag
     5161 gatgctgctt ctaaaataaa tatcaaacaa atgaatacat catgcaaaaa aagccagaca
     5221 accgacgtcc ttgcgtgtca gctgtctgag ctatgtttat ttattttgtt cttttaaaaa
     5281 gaacgataca catactgatt tttcggagca gagactttct cagcgtttgt aacaactaca
     5341 gatgggattt cacgcttgaa tggttgataa tagtctgatt ggatggttcc ttctatttta
     5401 taccattcat catttttaac cgttactcct tccggcatat gcgtcaacaa accaaagact
     5461 cctgaatctg ccacacaatg gataatgccg aatcggaaca agaagatatc tgtattgcta
     5521 tcgttcggtg tttggtaaac aaatccttca tagctgattt ttttgccaat gaattcgcta
     5581 gggtaattat aaattagctc cataacttct aaatagtttt catctgtgat cttgatagtt
     5641 tgcttatccc catacttttc tttcaactca ttcatttggt ctaagtagtc tgatttgttg
     5701 aaataaatac tggtatccgg ctttaagtat tgtgtctgca tctccggatc ccctactgat
     5761 tctttactta ctgggaaatt aaatcctttg gcttctacga tcgtagtgtc gagactaact
     5821 gttggaaaca acgtccccac gattactggc aaagctagca gtacataagc gattccacgt
     5881 tgataaggtt ttgacaaccc atggtcatga tcgtgctcgt gtgccttttc ttctgctttt
     5941 ccgtttttga cccacaaata cagctgaact aaagccaaaa gagcagataa aatcattgaa
     6001 agaatagcta agtagcgata atggacattg atatattgat cgagtttgcc agaaacttgc
     6061 aagtacatca tcaattcgaa atacccagat aaaatcaaaa accgcagcat attttccctc
     6121 ctaaataaat aatgtgaatc caataacgct aagggcaatc agtccgatca gcgataaaat
     6181 aaagcgtcct gtaaaatatc gtttcatcat cagtagattt ttgatatcga ccattgggcc
     6241 aaagaccaaa aaggcagcta caggtgctgt gccgaacaga cttaacaaag aagatcctat
     6301 aaacgcatct gcttctgagc aaagagacat cgtaaaggcc aataacagca tgatgataat
     6361 ggctaaaatc tttgttgagc cgagcgttag cataattcta gttggtaaat aagtctgcat
     6421 cgcagctgcg attaatccgc caatgatcaa ataacgacct gtatcaaaaa attcgtcgat
     6481 tgaatgtgtc aacacatgcc cagtttgatg gatcagctga ttttttggtt cattcttctc
     6541 actttgttct tcttcatgga catgatgatg gtgattttct gctactttag acgtgatttt
     6601 ttctttcaat acagcgtctt tttgaaaata ggcaagccag acaccaatca caagggcaac
     6661 aaaaaagctg cctatcaccc gccagcttgc catctgccaa gaattgccaa atgcgataaa
     6721 agtggaaaaa atcacgattg gattgatgat cggagcagtc agcataaaag caaacgctgt
     6781 gtgaactggt acgccttttt taacaaattg atgaacgatc ggtacgatgc cgcattcaca
     6841 tgaaggaaag aaaaatccta agatcgaacc agtaaaaata gagaagatac gattttcagg
     6901 aagccatctc ttcacacgtt ctggggtcaa aaatacttgt aaggcgcctg agataagaca
     6961 gcctaataat acgaaaggta atgcttcaat aacgatcgat aagaaaatcg tactcatctg
     7021 caaaaccgaa tttggaataa aagaaaacat gagtttcctc ctttttattt ctagttcatt
     7081 cgctttgcaa atatacgctt ttattcaaaa aaaacaagtt gctctgtaaa tttttacaca
     7141 actcttattt tttaccaaag cacttcaatt gactttttct gttattccca ttaaattaag
     7201 gtctcataca tatcacttct atcataaaag ggagaataaa aatgaaacaa accactaact
     7261 tgactgaagt tcaagacata ctgcaacagt catccgttgc cgtcatttat ctttcgatgc
     7321 ctaattgcag tgtttgtcac gctgttcgtc cacgactaga ggaacttctc acacattatg
     7381 atattccggc acttcatttg gatgcatttg aaacaccaga agtagctagt aaatttgaag
     7441 tgctgactgc gccagttgtc ctgatttttc atcaaggaaa agaagtcttt cggcaagcac
     7501 ggttcatcga ctttaaaaaa atccgttatc tactagatga actgacagca gaagatgatt
     7561 ccctttctta tgaagaaatc ttccgaacaa aataatcgtt gtccaaacag aaaaaagcga
     7621 ttgaacaagt gaaagttttg ccttcatttg ttcaatcgct ttttattttt attctgctgt
     7681 attttcggaa tgcgcatttg cgtcagaatg caagaaatag atcaaggttt gcaattcatt
     7741 cgtcaaatca acattttgga tcaatacatc tgacggagca ttcaatcgag cagcggtaaa
     7801 gttcaagatt cctttgattc ctgcttccgt taactgattg actacttctt gtgctctttc
     7861 agcgggtatc gttaaaattg ctacttcgat ttgttggatc ttgatttgtt ctgacatatc
     7921 ttccattgga taaacgggga taccgtctac gatgcgccca acgatttcct cattcacatc
     7981 aaaagcacaa ctgatacgta tgctattact ttgatggaat ttgtatttta gtaaagcact
     8041 tcccaagttc cctacaccga tcagcgcaac atttgtcaat tgatcgtcgt ttaatgtctt
     8101 tgcaaaaaag tgcataagat cttctacatc ataaccatac ccacgttttc ctaattcgcc
     8161 aaaataagaa aaatctcgac ggatcgttgc actatctact tgaacggctt cacttaattc
     8221 agttgaagac acttttttct ttccagcatt atttaaaatc cttaaatagc gataatacaa
     8281 aggcagtctt ttagctgtcg cctttggaat aatatgatct ttcacgaaat gacctccacg
     8341 tattgtctaa ttacatcttt gatttctcca tcatagcagt atttatagga aaaaatcaat
     8401 tccattgctc agatattgtg aaattttatt ctttattcat ttatttatca tttctgcaaa
     8461 tctcgcagta taatggaatt tatggtagac tactaattga aaaatagttt tttatttgag
     8521 gagaattcac aaagtagaaa agaggattcc atgatattat tacaagccaa tcagattgct
     8581 cgccattttg gtgcagaagt cttatttgaa aacatacatt tagaaatcag tacaggcgct
     8641 cgtgtcgctt tagttggcag aaatggcgct ggaaaatcga ctttattaaa gatcattgct
     8701 ggaattgaag cacctgatga aggaaagatc gctaaaaata aaacagcgac tctcggatac
     8761 ctcgctcagg acacaggact tgattccaat gaaacagttt ggaacgagat gctgaaagct
     8821 tttgaacaag ttagacaaat ggaagagcgg atgcgcgagc tagagatttt cattagcgaa
     8881 ggtacgccag acacacctgc ttatagttct tatttaaaag aatacgaccg gctgcaacac
     8941 gatttttcag atcgaaacgg ctatggttat gaaaatgaaa ttcgttccgt attgcatggg
     9001 tttaaattcg attcttcatt ttatgatcag aaaatcgata ctttatcagg cggacagcgc
     9061 acacgtttag ctttagctcg catgcttttg caacaccctg acattttgat tctagatgaa
     9121 ccaacgaacc atttagatat cgagacactt tcctggcttg agaattatct gcaaagctat
     9181 actggtgcat tattgattgt ttcccatgac cgttattttt tagataaagt agtcaatgaa
     9241 gtctatgaat taagcagaag aaaaatgcgc cattacaaag gtaactattc gaaatatcta
     9301 gatttgaaag cagaacagtt agcaagtgaa tggaaggctt atgaaaagca gcaaacagaa
     9361 atccataaat tagaagactt cgttgctcgg aaccttgttc gcgcctctac cacaaaacga
     9421 gcgcaaagcc gcagaaagac attagaaaaa atggaccggc tggatcgtcc tcaaggggat
     9481 gaaaaacaag caaactttct attcagtata gaaaagcctt ctggaaatgt agtcttgcaa
     9541 atcgaagatg ccgctatcgg atatgataat gtcaccttat ctgaaccaat caatctagat
     9601 atccgtcgtc aagaagcgat tgctctagta ggtccgaatg gtatcgggaa atcgacgttg
     9661 ctgaaatcaa tcattcacca gatccctttt atccgtggaa aagaaacgct agggacaaat
     9721 gtcaccatcg gctattacga tcaaggtcaa gctgatctgc atacaaacaa gacgatccta
     9781 gcagaattgt gggatgagca tcctaccact ccagaaaaag agatccgaag tgctttaggt
     9841 gctttcttat tttctggcga agatgtagaa aaaccaatcc ctctgctaag tggcggtgaa
     9901 aaagcacggg ttgctttagc caagttagcg atgaacaaag aaaatttttt gattttggat
     9961 gaaccaacaa accatttaga tattgacaac aaagaagtat tagaaaatgc attgatcgat
    10021 tatgacggca cactgctttt tgtttcccat gaccgatatt tcatcaatcg gatcgcgaca
    10081 aaagtcgtcg aactctctga aaatggcagc aaagtctatc taggagacta tgattattat
    10141 ttagagaaaa agcaggaaga agaagaaatc gccgaattgt tggcacaaga tgagccagaa
    10201 tctgctcctg ctcccacgaa caaatctgat tattaccaaa gcaaagaaca gcaaaagctt
    10261 atccgtagcc ttcaacgaaa aattactcag gttgaagaag aaatggcgcg gatcgatgaa
    10321 tggatcgatc agttggaaca agaaatggtc gcgccagaaa acctggaaga tcacgtaaaa
    10381 ctcaatgagc tgaaccaaga tttagaagct gctagacaag atcaagaaac aaaattgacg
    10441 gaatgggaag aactaagctt ggaattagaa gaatatgaaa attaacgaaa aaaccaaaac
    10501 agaaaccatt catgctgttt tggtttttct ttttttattt tatcaaacaa aaattccata
    10561 tttacctttt tcaccaataa gaagaccaat tactagtcat tctcactaaa gcttctaaat
    10621 aatagtaatc accccaaata caaggtgtat ttaccccatt atttttgggc ttattataaa
    10681 cagactgtgt taaaaagcca tcttcttctt ccgttgtagc atatctttct ttcaattgtc
    10741 gcataatttt ccaaacagca gactggtata agttcttttc tgttttgtct aatagacctg
    10801 attctattaa ttctaataat ccacaaacca tgatcaccat actagatgta tctttttctt
    10861 ctctgctaga atcatcaaag atcaaatccc aataacaaat ggtatcttct ggcaaccgat
    10921 tcataaaata attggctaat attttgctta aatcaaaata tttttgttct ttcaaataac
    10981 gataacttaa tgcaaaaccg taaatccccc atgcttggcc tctagcccaa caggaattat
    11041 cactataacc ttgatgcgta cttccatatc ttggttttcc atctttgaca tccatataaa
    11101 atgtatgata agttgaacca tcttctctta ctaaataatc tgctgcattt tgaatatgag
    11161 cctgagcagc ttttttatat ttttgatttc cagtttcttc actcgcccaa tacaataatg
    11221 gaagattcat gcaacaatcc atgatcattc taccttgttg tgaaggatca ctaggatctc
    11281 cccaagcttg tataactcct gccttctccc aaaaacgctg aagtaatgtt tctgcagcat
    11341 caattgaagc ttccttcatt tttcgatttc ccgttatttt ataatctgct acggttgata
    11401 aagaatacaa aaaaccaaga tcgtgatgat tagtgataat ttgacagtca atcctgtgga
    11461 taaaatctat atctgctttt tcagctgttt gttgaaataa aggatcaccc gtcttctcaa
    11521 ataacaacca attaattcct gtccaaaatc cttctgtcca accaatattg tctcttaatg
    11581 gataacgtcc ttttatagga cactcgcttg ggaattgata ctgactgtta ctgatacgtt
    11641 tgattaacct ctccgtgtgt ctgcgacaac gctcgatctc attattccat tctttttcca
    11701 ttaattcttt ataacattca tttatccatt tttctggatg tattatctct tcaaacatca
    11761 ctttatcaag catttcccca ccagccttta gcataatttt agtctttttt tattgttttt
    11821 attttggata gttagcccgg ttgtcccatc aaaatcaatt ttgggacgat tgttccatac
    11881 ttctaaacta taatcccgat cttgatgagc aaccactcct gttccgagcc aatgggttcc
    11941 ttttccaaat gtaccctcta atccaggaat aaaagtgttt tcccatgtca tcaagtttgt
    12001 attgggcaaa cccataatag ctgtggattt tcgtttaata gtatcgtcac ttaaacagat
    12061 aatacctgaa aagccagctt cactcaccct atagttttcc ttgtcttctt ctatgattat
    12121 tccttctttc tcaggagaac attcaatagc aaaccctgtc tcatatagaa catattctgt
    12181 atctaattct actttgtgta cgcgaatctg ccagccttca aaaggaataa gccaagttct
    12241 gatttttgcc tgtaaagtag gttcccatgt agaggcaagt acctctttct tacatacaat
    12301 gtctcgtaca ttatttcgaa cgatgaactg tccataggtt ttgatgctct cattcttaaa
    12361 actggtcgtg atatatgtgt ccgctcgctg aatccctagc attgaatcca ttgccatttc
    12421 tgctttcaat tgattcgctc gcggtatcga aaaaccaaat tgactagaat aagcaaattt
    12481 taaatatttt tcattggtca atgtatggaa atttggccct aattgtcccg cattcaaaaa
    12541 aaatgtatgt ccattatcat gttgtatgag caaattggct tctgaaagaa gtttagttcc
    12601 ttcacgaata ggcataggca tttctttagc cgtccaatac tcatggtctt caggtagcgc
    12661 caacaataaa aaaatcttat tcagccataa tggagataac gtactattat agggttcagt
    12721 caatgtcaat tgagggtagg tataaccaat attcaggata cctttttcat caaatatttc
    12781 tttttgcatc caccatctta tattccgatt gactaacccc ttgatttcac ctaatttaaa
    12841 aggcaaaaga tcggcccaca acatagctga ccaaaagctt gaaactgcaa aacgataaat
    12901 catgcttcga ccaaaaggca catttgcacc atcttccgca aaaaaatgaa tatagtctct
    12961 agaaaaatca atagctcggt tcttatatat ctggctaatg ggcttttctg gttctagtac
    13021 gctataaatc aaaccataat aatgaaatgc aaaaggatta taataatctt ggcgacctct
    13081 tgaactgtct cgataccagc cattttgaag ataacaattt tctatattat caaatgcttc
    13141 tttcagtgct gattctttaa ctggataacc aaatttttgg ataattgttc cgacaattat
    13201 cttaaaaaat tgccaattac catctgcaca ttcatattca aaaatttgcg agaaccaatg
    13261 aataatctgg cttttttctc ctgtatctaa ttttttccat aataattgtt catgatgata
    13321 taacgctaat aacattgcgg gcatttctac catacgctgg tctctttcat gtatttctcc
    13381 ccaataatcg ggatgggaag gatctgttcc agcttttatt cctgatagaa ttaatttcaa
    13441 ccatcgttca tctaattggt caattgcggg ccccgctccc cataaaaccc gagagaatcc
    13501 ttccattcct gcaattttat ttccatatcc taccccttga tgtatataat caatttgtgc
    13561 gccacttgct cgataaaacg gctggagtgg ttgatttatc tttttaaata agtcaaagta
    13621 atcctgtctg gtccttaata gtggtactgg atgtttcatt gtattttctc ctctttttac
    13681 taccaaatag ctaattccga ctgttctaat cgaagtaagc cttcaataaa aaaataatca
    13741 gaatagatca gtggcacatt ataagtcagt tctctatggt atgcttctgc tccgcctcca
    13801 atgatcccat cttcatccat attccaattt gcatagtgct tttctgtctt ttggagtata
    13861 ttgatagcag cttcatgata catttttgat cgtacagagg aaacatatcc tgctaattcc
    13921 agaagaccgc atgcagcgca tactccagca ctggtatcaa tattcgaatc ttttacagca
    13981 aaatcaatga gaggaatata accagttttt tcgattttct gaataaaatt tactgctatc
    14041 ttttctgcta tagctagata gtacggattt tttgtgtaac gataacttaa cgcgaatcca
    14101 tataaagccc atgcatttcc tctgctccaa caagaattct cattgtatcc ttgcccgcca
    14161 agtaaccgaa tatatttccc tgattctgca tcaaattctg cgatatgccc ggttgatcca
    14221 tctttcctca ctagatactt agctgttgta tctgcatgct gccgagcaat tttagagaac
    14281 ctcggatcac cagtttccct cgaagcccaa tacagtaatg gtaaattcat catactatca
    14341 ataatcatcc aaccaggttt atggtcattc catgctacta aatagttccc tgaaatatta
    14401 tagcgtccag caagtaccgt agctgcatgt aaccctagtt gtcgactatg tttgttttga
    14461 gactgttgat aatgtgcaat agccgatggt aagaataaaa aacctacatc atgatgaacg
    14521 tctgaaaaat ggtacagggc agattctaat aattgttctt gttgtattgc tgcctctttg
    14581 aacttttctc gttttgtctt gtgaaagagc tgccataaaa taccactcca aaaaccattg
    14641 gtccaccagt cgattcctct gtcctttatt acgtctgtat attttccgtt gtaagaaata
    14701 tatggaattt tattccccat tctgtctaat tccgcatcta tcttttcaga agctacattc
    14761 aaaatttttc ttgaccattc cttcgtctgt tcattcatgg atatcccgcc attcttttaa
    14821 tttttatgaa tgtggagttg ctcctagaac gaactagatg gttaacattc gttatttttt
    14881 ttacttattt gcagctccaa tgtcattctc cttaaattta gactgcgccc acacataaat
    14941 caagaccatt gggatcactg aagttgctaa aattagcata attgcgccat aattaattgg
    15001 ctcacctgta ctagaaaccg tcatattttt gataattaag ggaaccgtat atttatcttt
    15061 atctgaaagc atgactaatg gaaatagata ttgattccaa cttcccataa agataagtaa
    15121 accactagta ataatcgtag gtgataaaat cggtacagcg attttcgtaa aaatcaccca
    15181 ctcattggca ccatcgattc tggcagcctc catcagtgct gtaggaaatt gactgctata
    15241 ttgtcgcatc ataaacactg aagttgtact ggcaatcgaa gggatgatca cacctgcata
    15301 agtattcgat aatcctaaac gactcataat gtaaaataaa ggaatcaatg tggtaaaaaa
    15361 aggcaccatc ctagatagca taactgtatt gaacaacagt ttattcccca taaaacgata
    15421 ctttgctaat gcataacccg ccattgtaga actggctgta gctaaaactg tactgattac
    15481 agcaataaca atcgaattcc aaataatcaa gggataatta tatctcgcca tgatggcttg
    15541 ccagtttttt aatagcatat ccccaaactt gaaattgatt ccgccactta aaatttcttg
    15601 attactttgc gttgaagcta caatcatata taaatatggc atcaaaaata ttaaggcacc
    15661 aaacacaata atgacgtacg taaaaattct gctttgtatc ttttgtttag tcatttttat
    15721 cacctccgaa tttaaattga atagccgata atataacaga tacaaacacc atcgtccagg
    15781 caatagcaga ggcttttcca aaatcaacca tttcaaaact tgttttatat aaattgactc
    15841 ctacgaccaa agctgaattt tctggtccat tggaagtccc aaagaatata tttgggatat
    15901 tgaaattacc caagccattg atagtggcta aaactgtggc cattaaaatg attggcttta
    15961 gcatggggat cgttaataaa aagaattgcc gaaatttcga tgctccatct atttcagcac
    16021 tttcgtatat ctctttagaa atcccttgaa gaccagctaa aaataaaata gtgttatatc
    16081 ctacactaag ccaaatcatc gtgatgatca ttgatattcg tatccaaaaa ggatttgtta
    16141 accactgtac accgttaatt cctagcgcat tcaaaaatcc attgaataac ccagtcggat
    16201 tgaaaaaagt catgaatacg gaagctacag ccaccgttga tgtaatggtt ggaatataat
    16261 agacagttct aaaaaatcct tttcctttta actgtgctga attcaataaa acagcaaagt
    16321 aaatagctaa tagcatcgtt attggaattg atcctgccat gataatcaaa acattcgtgt
    16381 aagatttaat aaacaatgga tctcggaaga tagctagata attgtcaaat ccaacaaatt
    16441 caaatgagta gccaattttc ataaaactaa aagcaaacga gataatgatg ggaatcaaaa
    16501 tcgtcacaat aaagaaaata acaaaaggtg tgaccaatcc ataagcactt tttttatgag
    16561 acgtatctgt tgcttttttc ttgctgttct ttattgtctt gcttggtaac gaagattgca
    16621 tcatcgttaa gtcctcctaa tattttttaa tcaccagtta attttacgcc gtacttatga
    16681 gcaaaatttt ctgcttcttt tagatatgaa ttttcaaaat cacctgtttg ccaatagtta
    16741 tatgcagcgg tagtcaaata agacgtagca tctgaataag tggctggtaa aacagtattt
    16801 gatgtatttt cattgatttt attcaatagt tcatagtatt tttgtcctcc ttagtagtca
    16861 aactttttct cagctgcttc tgtttgatag gccaatgagt tagatgtagc cacgcccagc
    16921 tccagtgcgt tggctaagca ttcttcatct gtcagcataa atgtaagaaa ttgttgtgcc
    16981 acttgtggat tttttgcatc tttagggaca taaaaacttg atcctcctga tactgcacct
    17041 tttcctggat tttcttttga aaaaggaatc agtggagcca tgcgccattt gtcagatgca
    17101 tcaggaaaat tcaaagacat attcgtagca taccagccgc cagcaacaaa cattgcagat
    17161 tcttgaaatg ctttctcagc atcttgtgca ccgtagaaat gtacaatatc tgagtcgatc
    17221 atttctttga ttaactttgc agcatgtttc gcttctttag tattcagacg gatatttcct
    17281 tcttcatcaa gcaaagattg attctgctgg gctagcatgt tgataaacaa ttcttggtcc
    17341 ccagattctg gcaaggcaat tccaaaacta ccagtttttt cttttaatgc ttttgccacc
    17401 tggatatatt cttcccaaga actgatttct tcaaaatcta cacctatttc agcaaatttt
    17461 tttggttgat aataacaaag tgctggggta aaatcactag cccaaggaat aatcgtttca
    17521 ttactcgttt gttgacgcaa aatattcaat ttttgatcat aaaactgatc gccatatttg
    17581 tcataaaatc catattcatc agcactataa aaagcatctt gaaatttttc cagcacacct
    17641 gctgctgaag tgtctgccat ggagataata tccggcatat cttcgtgtgt taccaatttt
    17701 ggggtgatca tttgtactgt tgctgttccc gaagctacat tcacaatttt taagtcaata
    17761 tttgctccag ttttgcggtt atagattttt gcagcatttt caacatagtt tgtcagtttg
    17821 ccgtttgcga catatacttc taatgtagtg gcttttttta tcattggatc tgcgatgttt
    17881 gctgttactt cttcgttagt ttttccacat gcacttaagc tgaataatag ggtaatgttt
    17941 agcaaagcta tcaccaattt gtatcttttt ttcatgtcta tcccttcttt cttttcaagt
    18001 tcttatataa aacgctttca cttatgtata ttaacggaat tagtaacaca aatatttata
    18061 taaaaaaata ttttacttgt cgttttggct tttcattttt gttataatag tcattaatta
    18121 taaatagaaa gctttttata taaaaagaga aaggaaagtg gttcttttgt taacaaacaa
    18181 ccaatcctcc aatcttgtca aaaaaacact aacctattcc atagataccc atcttcattt
    18241 atctgctgct ccctattttc gactactaat aaatgaaagc gctaatttat atgctgaatt
    18301 tgcacataaa tctatagaac tagataatcc ttccctttta ttcattgata aacagcaaga
    18361 agtccaaatc ttttctactc aaaaggaaac acaaactttt atcattatcc agttttattt
    18421 tgatgagcaa ttattagcac atattacggg acaatctttt tctatagatc aagaaataac
    18481 atctatcatt acttttctga aaaatgaaca gttcaaaatt gattatctaa aacatagtaa
    18541 gccagctatt tcctatgatc aggaaatcaa ttcttttttc atactatgcc aacagatgag
    18601 cattcatttg ctttccttat tagttactta ttgtctgcaa aaaattcaat tgtcttcctt
    18661 accaaggcat tatcaacagg aacttgcttt attgacaata ccttcaaatg agaaagtcat
    18721 tcctaaaaaa aagcatattc tttattcaga tgaattagtt gatccaatca tacgatattt
    18781 agaagaacat ctttcagaaa attttttgct tgaacaatta gcccaaattt tttttatcag
    18841 tccagccagc ttaaaaaaac attttagaaa agtgaatgga aaaagtgtta ttagctattt
    18901 taaagaacta aaaatggaaa aagcaaaacg tctcgtgcta gaaaatgaat taacctatac
    18961 agaaattgca gatatattag gatttcattc gatccatcat ttttcttcag cttttaaaaa
    19021 atatacaggg ttatcccctt caaagtatta tctatcctta aaatagaaaa aaatttttag
    19081 gttttatcca caaattattt ctctatattt tctgtctatt caaaataaac aataattttc
    19141 cggcacaatt atttgctgtt ttttaccata acatagtacg atcaacgtag aaaaggagat
    19201 gtttttatga aagttgttgt cattggcggt gtagctggcg gtccttcatt tgccactcgt
    19261 ttccgtcgat tgaatgaagc gcacgaaatc attatctatg aacgcggaga aaatatttct
    19321 tatgcaagtt gtgctttgcc ttattattta ggaggtgtga tcactgaccg tgactcgctg
    19381 atcgaacgta caccagaaat attgaaaaca aaaaacaaca tcgatgtctt tactaaacac
    19441 gaagtaacag caatcgatcc ttctactaag cgattaacag ttaaagacct atccacaaat
    19501 gaagaaataa aaacagatta cgataagttg atcatctctt ctggtgctag accagattat
    19561 ccggatattc ccggagtttt tgaagcagaa aacggctttg tcctccgtag tgtgacggat
    19621 gcggatcgaa tcaaatcgtt ccttgaagaa aaaaatccac aacatgtcgt cattcttggt
    19681 gcaggtgtta tgggtctgga attagctgag aatttcaagc atcgcggctt agacgtgact
    19741 ttaatcgatc aattgccaca agtcgctttc ccttatgatc cagaaattgc taatttagtt
    19801 tatgacaaat tgctgaaaga aggattagcc gttcatttag aaacaagagt tactgagatc
    19861 cgtgataaag gtcgagaaat agtattatca gatggctccg tcctttctgc tgatatgcta
    19921 atttttgctg ttggtgtttc tccgaagaat gaagtggtga aagcaaccgg catacaatta
    19981 tcagatacag gtcagatcat tgttgatgac cagttacaaa ccaatctgcc ggacatctat
    20041 gcgattggcg atattatcga aacgactagt gtagtgactg gtcagccgat ccaaagtatg
    20101 ctttccagtg cggccaatcg tcaaggacat atgctggcag atattttaaa tggtacgcct
    20161 atgcgctatc gcggatatat tggtgcaggt gtcgcaaaaa tctttgatca tacagcaagt
    20221 tatgcgggaa tgacagaaca tgaattaaaa gcatcaggca taacaaatta taaaactgtt
    20281 tttatcactc cctttgacca tgcctatttc tatccagaag ctacaagatt aaatctaaag
    20341 ctgatttttg atgcagatag cggtcgtatt ttaggtggac aagcatttgg agaaaaaggt
    20401 gtcgataaac ggatgggaga actttctgta gcaatcaccg gaaacttgac agtctttgat
    20461 ttgcctgatt tggagttgcc ttactctcca ccgtattcta ctacccgtga cccgttgaat
    20521 atagctggtt atgtcgcaat caatcaaatg acgaatatcg tagaaacgat caaagcaagt
    20581 gatatacccg aaaacgattt gaaagaagcg ttctttttag atatacgtga acctaataaa
    20641 gcaccttctg gcagtatctc ggcaacaaaa aatattccaa tgaatgagtt acgagatcga
    20701 atcaatgaaa tcccaaaaga taaaaaagtt tacattactt tcagaagagg attgaatact
    20761 tatacttctg cccgaatctt ggcaggtttg ggtatcaaag cgattttgat tgaagaataa
    20821 aaaaacgacc gcggtgggta gccgcggtct aggagttatt tttacttgag gagtaaaaat
    20881 gaaaaagtat atagggttgt tggttggtat gtatatagaa tagaagataa atgtgaagaa
    20941 attgtgcatt atttcttcac atttattagg ttatagttta agaaagttta aacattgcgc
    21001 aatttaatta ttttagctgt ttcttttcat aaaaaaacat gttgtgactc tgagacaaat
    21061 catagtccat agtgatgtct ctagagtaac tacgcctaat attacgaggc atcatattaa
    21121 tctatcagca ctagtaccta aactagcatc cctttcgaat attttttctt atataataaa
    21181 gtttgctttc actcctctaa tctcaccaaa tcgactggct tgcgttgtgt gatactagga
    21241 actgattggt ttagaacgac ttttctattg taatcatatt tttcgccatc ctcgttgtct
    21301 tctaatcctt caatgaatga tccctaatgt gtgtattaaa taatttaatt tttttgttcc
    21361 tatttattta a
//
LOCUS       DBNICI010000032        20903 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.032, whole genome shotgun sequence.
ACCESSION   DBNICI010000032 DBNICI010000000
VERSION     DBNICI010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.032"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(93..536)
                     /locus_tag="AC1ZCC_000987"
     CDS             complement(93..536)
                     /locus_tag="AC1ZCC_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HIQ1629254.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(754..2094)
                     /locus_tag="AC1ZCC_000988"
     CDS             complement(754..2094)
                     /locus_tag="AC1ZCC_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIQ1629255.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2363..3259
                     /locus_tag="AC1ZCC_000989"
     CDS             2363..3259
                     /locus_tag="AC1ZCC_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HIQ1629256.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3409..4350)
                     /locus_tag="AC1ZCC_000990"
     CDS             complement(3409..4350)
                     /locus_tag="AC1ZCC_000990"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HIQ1629257.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4476..5597
                     /locus_tag="AC1ZCC_000991"
     CDS             4476..5597
                     /locus_tag="AC1ZCC_000991"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HIQ1629258.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5855..7357
                     /gene="eat(A)"
                     /locus_tag="AC1ZCC_000992"
     CDS             5855..7357
                     /gene="eat(A)"
                     /locus_tag="AC1ZCC_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HIQ1629259.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7424..7951
                     /locus_tag="AC1ZCC_000993"
     CDS             7424..7951
                     /locus_tag="AC1ZCC_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIQ1629260.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8162..9223
                     /locus_tag="AC1ZCC_000994"
     CDS             8162..9223
                     /locus_tag="AC1ZCC_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HIQ1629261.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9226..10122
                     /gene="murQ"
                     /locus_tag="AC1ZCC_000995"
     CDS             9226..10122
                     /gene="murQ"
                     /locus_tag="AC1ZCC_000995"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HIQ1629262.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10136..12118
                     /locus_tag="AC1ZCC_000996"
     CDS             10136..12118
                     /locus_tag="AC1ZCC_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HIQ1629263.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12183..12629)
                     /gene="nrdI"
                     /locus_tag="AC1ZCC_000997"
     CDS             complement(12183..12629)
                     /gene="nrdI"
                     /locus_tag="AC1ZCC_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HIQ1629264.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12840..14264
                     /locus_tag="AC1ZCC_000998"
     CDS             12840..14264
                     /locus_tag="AC1ZCC_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HIQ1629265.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14445..15218)
                     /locus_tag="AC1ZCC_000999"
     CDS             complement(14445..15218)
                     /locus_tag="AC1ZCC_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIQ1629266.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15215..16120)
                     /locus_tag="AC1ZCC_001000"
     CDS             complement(15215..16120)
                     /locus_tag="AC1ZCC_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1629267.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16274..16594)
                     /locus_tag="AC1ZCC_001001"
     CDS             complement(16274..16594)
                     /locus_tag="AC1ZCC_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIQ1629268.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17068..17754
                     /locus_tag="AC1ZCC_001002"
     CDS             17068..17754
                     /locus_tag="AC1ZCC_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HIQ1629269.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            17756..18700
                     /locus_tag="AC1ZCC_001003"
     CDS             17756..18700
                     /locus_tag="AC1ZCC_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HIQ1629270.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            18899..19543
                     /gene="pcp"
                     /locus_tag="AC1ZCC_001004"
     CDS             18899..19543
                     /gene="pcp"
                     /locus_tag="AC1ZCC_001004"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HIQ1629271.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            19622..20899
                     /locus_tag="AC1ZCC_001005"
     CDS             19622..20899
                     /locus_tag="AC1ZCC_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629272.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         6753 a         3767 c         4344 g         6039 t
ORIGIN      
        1 cttattcacg gcgttccaga agtagcttct tcttatttct tgaagctgat aacttctgga
       61 acatcctctt tttttatttg gacggataat ggtcattcca tttctttgat catttttttc
      121 attttgtcga tctcttcttc ttgtgcctga atgatttctt ccgctaattt ttttacttca
      181 ggatctttta aatcagctcg tttacttgcc aggatagcga tagaatgatg aggaatcatg
      241 gccttcatcc aagctgtgtc gttgacagta gtttggcttc gtaccataaa caaggaaccg
      301 gcaaaaacaa gaagactagc ggctaagatc ataacattta tctttttgtt ttcatacatt
      361 ttccacatga acaacagcat gatgactgcc atgactgcac ccatcatcaa cgccatgaat
      421 actcgtgtac gactgaagaa gatatgatcg agcgcataga cattcaagta catcatacca
      481 aacataacta ccgttgaaac accgatcatc aacaaaaact tccaatactt tttcatttaa
      541 aacaaactcc tttttttcat tcctttcttc tatcatgtca tgagcatgga gaacaatcaa
      601 ttattttgca cagagggttt ttgaacaccg tttaccaaga ggtcatgaga ctgtttattc
      661 ggaaaaagga gttgcaacaa gacttttgtc acaactcctt tcttcaatag ctccatctat
      721 tccacataaa taaaataaaa ttttctatcg atttcattca ttcgtcaata cgaacgcatc
      781 attctttttt tgataactga aataaatgaa caggtaaagc aggccagtca atacagttac
      841 cccaatggag ttcgtgaaca ggataccaag gaaaatcaat cctaagacag ctaatgcaaa
      901 aaagctgatt cctttatccc ataatgttcc ttttcttagt gccagtacaa aggatgccgc
      961 actgataatg atccagatca ataatactgt atacgaaagg gaacctgcca gatagccaaa
     1021 taatttgtca ccgacaaaat aagaaagagc tacaccaaga taaagaacac tggtacaaaa
     1081 taaaacagca cgttgcggta cttgatgctt gtttaaaaca gctagttttc tagaggaacc
     1141 ttttcgatcc ttcaaacgga agaacaaaat tcttgaagat gcatacacac ccgaattgat
     1201 agatgagaac aaagcaagga caatcacaaa attgacgata tccgctgcaa atgggatatt
     1261 cattttttca aataccatca caaaaggact gacattcgtg cctgctaaag tattccaagg
     1321 aaaaagaatc aaaagcaaaa acaaaggaat gatgtaaaag gaaatgattc ttcccatgac
     1381 acctttgatt gctttaggaa tcgcacgttt aggatcatct gcttcgctta ccgtgatagc
     1441 aatcagttca gaaccgccgt aagaatagat aactacaagc aaagaactga tgattccctt
     1501 cataccatga ggagtaaacc caccatggtc agtcaattgc tgaagggtag ggaaaacgcc
     1561 tgtgcccaac atttgctgac cgacaagata agtagcaaaa atgatcagta agataatgac
     1621 actgatttta gcaaaagcca gccaatattc tgtttctgcg aataaccgta ccgaataaag
     1681 attgatgatc gtagttaaaa cagccaacat aaaaacaaat acccaggctg gaatgtgcgg
     1741 gaaccacaac tgcaaaaagg aagcagctgc aacagcttca gcgatgatat tgataatcca
     1801 catagaccag tagacccaat cagtaaaatc agcagaatga gcacctagat aagggcgtat
     1861 caaaccagat aatccgtgat gcgtgtcgcc accgcttaaa acgatttttc ccagaccttt
     1921 catcacgata tataaaatca gcccaccaat caaaaaagca atcaatacag aagggccggc
     1981 gatcccaatc gcttcgccac ttcctttaaa tagacctgca ccgattgctc cacctaaagc
     2041 taacatggtg atatgtctgg agctaagttg ttttttcaat tttttatctt ccataaatga
     2101 cctctttctt ttaaattcac aatcaaaatt tatattaaga ataaatagca atatttaaca
     2161 atgactaatg ataacaaatt ttcagacaat tacaactatt cagaaaactt ttaggcgaat
     2221 tttaatagaa gaatgattta tttcatcata ttttcataca aatgttaaat tttcatacaa
     2281 atataggaaa cgttttactg gctttttact atttaaacag gctatcatag gaaaaagtga
     2341 cagagaaagt gaggcggaaa agatgaacaa aaatgacgta ctgctagttg caaaaaataa
     2401 atcactttat tttttgcctt ccatccgctc tttagatgtt tggtccggaa aagatttact
     2461 acagtcggaa ttcattaaat cagctagaca acagttggca aattatcagc tgatcgtttt
     2521 tttagattat ggatttgata tggagatggc tcgacgcgtg cgcccattta ccaatgcaaa
     2581 aattgtgtta tttttttgga atcacttcaa agcagaacac gaggcgatgc tgaagcaggc
     2641 acaagatgaa ccggcaatcg atgagattta tcattttgat tttttggaag caaaagaact
     2701 tgggctgaaa cataatagtt cattttattc gaaacatatg aatttttctt ctagcgaacc
     2761 aaagacggat ctattttttg gtgcaacgaa taatgggaga aaagaacgag cagaaagcta
     2821 taagcaggaa tttataaaaa gagatgttac gaccaactat ttcattcttc cacatcgagg
     2881 gaatgaacaa cctggatatc ttacttatga agagtacctg aaaaaaacag gggaatcgcg
     2941 cgggattctg gaactgttgc gggagggtca gcaaggagtg actttgcgga catttgaatc
     3001 catgtatttt caaaagaaac tagtgacgga taatcaagcg attgctcatt atcattttta
     3061 tcgtcctgaa aatatttttt tgcttcgaga acgtgattta gatgaattac cggaatttct
     3121 gcatacacca tttaagccaa tcctatcaga gctattggaa ttttatgatg cagaaaattg
     3181 ggcaaaacga tttatcacta acgattccac tatttatgag caatacgaat atgacctctc
     3241 ctctgttgaa aacaaataaa aaacctacat tttgaagaga ttatgtactg aaacaaacaa
     3301 gcacaattct tatggcatca tgagagttgt gttttctttt tgatctttta ttgggcttaa
     3361 aaaaagagaa ccagctgctg attcagctga ttctcatcag agagcctttt attctgcgcc
     3421 aaggattcct tcttttgcat ggactaattg ggtcaaaaag gcacaatatg gtgtagcaat
     3481 atcatatttt tgtccttttc gccaaatggc accattgatg tagtcgattt cagtcaaacg
     3541 atggtttttg atcaggtctt ggtacataga tgggtaatgc aacccgatgt tgtcaggatc
     3601 ataagtagaa gcaatatgtt ggtacacttc ttcttgatct agtatgattc cttcttttgc
     3661 tgcgatagaa gcaaattcag atacgattgt tcgaaccatg ctctctgccg ctttttgcgc
     3721 gcctagttca gcaatattac aatccaaaat cgtgcataaa ccattcaatg ttccatttac
     3781 acaagctttt cgccaaatgg aataacgaac gttatggcta tagatcggat ttaaatttgc
     3841 ttcttgaaag acttctacaa ctttttttgt aaatgcttcg ccttctggtt ccaagttctc
     3901 tagttcgatt tctccatcac ctaacaatgt gacttttccg ggaccagcta gaccagctgt
     3961 ccacatcgtg atcccaaata agatattttt ctttggcaca tatttttcca gtacatcttc
     4021 atgacctaat ccatttagta aacacaaaac ataagtattg tcagtaataa tggattggat
     4081 cgaacaaaac atatcatcta attgattggc ttttgttaat gctacgatca agtccacgtg
     4141 ttcgttgctt tcgatgattt cttctggtga atagattggc agttttgcga ccacttcttt
     4201 accgttaaag tcagcaatca acccatttgt acgaatggct tcaatgtgag caggccaacg
     4261 atcgatcaag aggacctcat ttccgctttg atgtagcatc aatccgattc ggctgcccat
     4321 tgctccggcc ccagctattg cgattttcat gttttttctc tcctttattc aatgagttcc
     4381 cttttagtat atagaaaaat ggaagaaaat agcattgttt tgtttgaaaa aaatgaaaaa
     4441 aagtgtctta cttaagaaga aagtgaggga tacagatgga tattttggcg gcaattgatt
     4501 catttaaagg aagtgcttct tcatttgaac taaatcaggc agttctaaat ggctgggaag
     4561 atcgtaaagg aagaaaaatc aatgtgccga ttgcagatgg cggagaaaat actgcagaag
     4621 caatctatta cgcgctcagg ggaacatggc gtaccattga tgggatggat ctcttattcc
     4681 gtcagcgaaa aatcagctat ttattgacaa cttggcaagg aaaaaacacc gcagtcatcg
     4741 aatctgcaca agcattgggg ttagatctac ttgataaacc aacggatcaa actatccgac
     4801 ttgcttccag ttatgcctta ggagaactta ttaaagatgc actgcttcaa gaagtagaac
     4861 aaattattgt cactttagga ggaagcgggt gtagcgatgg tggtctagga ttactccaat
     4921 ccttgggtgc ttctttagaa gggtacacag aaggaaatcc tttattatct actaaaaaac
     4981 ttcattttga tcgaagaaac gttcttgttt ctaaagtaaa attgattatc gctgcagatg
     5041 ttaccaatcc atatacaggt caacaaggag ctgcttggca atttggaaga caaaaaggag
     5101 gaatagatac gacgctagct ttcttagata aacaagcagg aaagattgct cagcaaataa
     5161 aagatttctg ggaggtggat ttagatgata ttccaggaag tggagcagca ggcggtattg
     5221 gtggagccct ttttttgttg ggagcgaaga tggtttctgg tttcgattta gtcagccaaa
     5281 tcactggatt ggaaaaaaag atcgaacaag cagatgtcat cattacagga gaagggcgga
     5341 tcgatcagca aacgatccac ggaaaagttc catacggtgt agcagtgctt gcaaaaaaat
     5401 atcgaaaacc aataatcgct atttgcggaa gtagagacca agagctagaa ggactttctg
     5461 attttctccc aattgtctta agtattcatt tgggtccaat aggtttagaa gaagcaatgg
     5521 aacacaggca aacattagaa aaaacaaaaa tcttaggaca gacacttagc agatttttca
     5581 ccatcctccc gaagtagttg acaaaatcat tttctttatg ctatcgtaat ttcaaatata
     5641 ggacaaagtg agggatccgt gcattgaaaa attaagtcga ttgatgaaag ctgcattcag
     5701 actgtacctt gtggagacaa ggaagcagtg tgcagatttt cgggattggc ttggtttttt
     5761 cgatgtatgg gtctttttgt gtgtatccaa cccttgcatc ctctttagcc agttctgaaa
     5821 ataggactgg tcttttttta cgaggtgatg gaaaatgtct aaaatcgaaa taaaaaatct
     5881 gacattcggc tacgacagcc aaggcacatt attatttgaa caagcaaatc taaattttga
     5941 cacacaatgg aaactaggac ttatcggacg aaacggtcga ggaaagacaa ctttactgaa
     6001 tattctacaa aacaaactac cttatcaagg gcaagtaatc catcagcaag aatttgccta
     6061 tttcccgcaa cagacaaaag ataaagaacg tttaacctat tacgtgttaa atgatattac
     6121 ggattttgag atatgggaaa tcgaaagaga gctccaattg atgcaaacag atcctgaaat
     6181 cttatggaga gaattcagca cactatcggg gggagagaag acaaaagtcc tactggcact
     6241 tttatttgtg gatgacactc atttcccgtt aatcgatgaa ccaacgaatc atttggatat
     6301 ctctggtaga aaacaagtag cggcttattt gaaaaagaaa aaacaaggct tcatcgtggt
     6361 cagccatgac cggggattta tcgatgaagt agtggaccat gttttagcaa tcgaaaaaag
     6421 tcaactggaa ctttatcaag ggaatttctc tatctatgaa gaacagaaaa aacttcgtga
     6481 tgaatttgaa atggctcaaa atgaaaaatt gaaaaaagaa gtcagtaggc taaagaaaac
     6541 agcagctgaa aaagccgaat ggtctcgttc ccgagaagga gataaaacaa agaaacaagt
     6601 cggattcatc gatactgaat ctagacgagt gaataaagga gcagtgggtg ctgatgctgc
     6661 acggacgatg aaacgatcca aagcaatcgt gaatcggatg gagacccaga tcagcgagaa
     6721 agaaaaacta ttaaaagata tcgaatatat cgattctttg acgatgaata gccaagcgtc
     6781 tcaccataag cgacttttaa gcgtagaaga tcttcaatta gggtatgaaa atctgttatt
     6841 cgagccaatt cattttacaa tcgagcctca tcagcgggtg gcgatttcag gtcctaacgg
     6901 tgcaggaaag tcatccatta tccattatct tctgggggca ttcaacggca aggttatagg
     6961 agaaaaatac cagccaaaac atctgagcat tagttatgca agccaaaatt atgaagacaa
     7021 tcgaggaacg ttggcggaat ttgcagagaa aaaccaagta gactaccaag catttttgaa
     7081 caacctccga aagcttggga tggaaagaga tgtttttcat aacaagatcg agcagatgag
     7141 tatgggccaa cggaaaaaag tggaattggc taaatcttta tcacagccag ctgaactata
     7201 tacatgggat gaaccattga attatttgga tgtcttcaat caagaacaat tagaacaact
     7261 gatcttgaac gtgaaacctg ccatgttact agtggaacat gatcaaacct tcctggataa
     7321 agtatctact gagattattt ctcttgagag aatctaaccc ttgtaatctc aagaacaaca
     7381 aggtatggtt aaggggaact ttgccaacga aggagagaat cacatgcttt atgtaacgag
     7441 acacggagaa acaacttgga acgcacaagg attagtttgc ggacgggcag atgtgccgct
     7501 gactgaaaaa ggacagatgc aggcacagaa actagcagaa aaggtagtag atttgcctgt
     7561 tccaatcacg aaaatcatcc attcccctct tcaacgagca agagatacag cacaagcagt
     7621 tgctgatcgt ctcagcttgc cgctgaccgt tgatgaacga ttggtagaaa tggattttgg
     7681 ggattatgat ggactgccaa gcaaagatga gaactttcaa aaagcacgtt tagcgttcgc
     7741 tgtacgattc ccaaatgggg agtcagtctt agatgtgtat gcacggatcg ttcctttatt
     7801 gaaagaatgc atcgaagacg aagaaaatgt ctatctgtta gtttgccata atgccttgat
     7861 aagagtgatc aatgcttatt ttcatccgat gcctaatgag gggttcttta cattcatggt
     7921 agataataca gaattggttt cttatgagta attatgtttt tgtgttattt aaataaaata
     7981 tttcaaggca ctgcttttgc ttagcagtgc cttttttagg ttatgacaat tcttttgata
     8041 atataaactc aacctttaga aatgaaattt aattatttta ataaaagtat tgaaattttg
     8101 tttctttaga tatatactct agtcatagaa atgaagacgg atacagaaaa agagggataa
     8161 tatgtttggc ttttcaatat ttatgaacga ttctttgtca gaagagaaga aacagtatat
     8221 ccgccaaatg gcatccaatg gctttgtcgg tattttcact tccatgcata taccagaaga
     8281 tgatgtttcg gcatatcaaa agcgattgat cgaactagga agcgaagcga aaaagaataa
     8341 actagaattg atggtcgata tttcaggaga ggcattggaa cgagcgggtt tttcattaga
     8401 agatctaacg cctttgaaag caattggtgt aacaggttta agaatggatt atcatatttc
     8461 caatcagcag attgccgact ggtcacatca attgaaaatc agtttgaatg ctagcacgat
     8521 cacgcctaaa gatatcgatg aattgaaaga agcagaagct gatttctcac agattgaagc
     8581 ttggcataat tattatccac gtcccgaaac agggctggac aaagaatggt atcagaaaaa
     8641 aaatcaatgg ctcaaaattc aaggtctcct tgtccaaggt tttgtaccag gagatacaga
     8701 gttgagaggc ccactatatc aaggattgcc aactttggaa gaacatcgag ggatccatcc
     8761 tcttgcaggc gctctagatc tattagcaag taacacggat attgtttaca tcggagatgc
     8821 aggtctgtca gaaaacgttc aggagcaatt tgcttctttc caaaaagaac agacagtctt
     8881 gctccatacg gaacctgtag atgaagaatt ttacgaatat atattaggga agcatacgaa
     8941 tcggcaagac gatgcaagag atgtcatacg aagcgtagat gcacgttttc gagagattcc
     9001 gcctatccca gcaagaaata cagctacgcg tatgaaaggg tctattactt tagataacga
     9061 aaagtatttg cgctatatgg gtgagatcca attaaccaaa tatgatttgc cagcggatga
     9121 aaaagtgaat gtagtagcta aagttatcaa agaagagcta ccattgatca accaaatcaa
     9181 agctggtatg aactatcaat ttataagaaa agaaggaaga taaaaatgag caaaatcaat
     9241 ttagagaacc taacaacgga acgcagaaac caagaaactt ttggattaga tgagatgagt
     9301 atttctcaag ccgttcaact aatgaacaaa gaagatcaga aagtggccgc tgcggttgaa
     9361 aaagaactga caagtatcga accagtgatt gctaacacga ttgagtcctt taaaaaaggt
     9421 ggacgtctga tttatatggg agccggaaca agtggaagac taggtgtgtt agatgctgct
     9481 gaatgcgtac cgacattcgg tgtagaaccg gaaatggtca taggtctaat tgctggtgga
     9541 gaagaagcaa tgactgtagc tgtcgaaggg gccgaagatt cttatgagct agggcaagaa
     9601 gatctaatca agctgaattt gacaaaaaat gatatagtta ttggtatcgc agctagcggt
     9661 cgtacaccat atgtgatcgg tggattagat tacgcaaaat cgatcggtgc tgtcacagga
     9721 acgatttctt gcaataaaca agccgaaatc agtaaacacg cggatttccc aatcgaagtc
     9781 gatgcaggtc cagaattttt gacaggctct actcgattga agtcaggaac agcacaaaaa
     9841 cttatcttga atatgatttc tactatcagt atgattggta taggtaaagt attcaataac
     9901 ttgatggtcg atgtcaaacc aacaaatgaa aaactaatcg aacgctcaaa acgaatcatc
     9961 atgcaagcga cagaagctga ttacgaaaca gctgaaaagt actttgaaga agcagatcaa
    10021 aatgttaagc tggctatcgt catgattttg acaaatagtg ataaagagga agctcaagca
    10081 aaattagctg aagcaaacgg atttgtaaaa gggaccttat gagggggaaa agaccatggc
    10141 aaaaaacgaa ctgactttgg aagaactggc aaaacaaatt tataaagggg caggcggcat
    10201 gggcaatgta gaaagcattg tccactgcat gactcgtgtg cgtatgaaga tcatcaatga
    10261 tgatcttgta aatgtctctg aattaaaagc gattccagga gtgcttggtg tagtagaaga
    10321 tgaacagctt caagtcatca tcggacctgg gaaagtgaac aaagtagcac agcaaatggt
    10381 tgatttggca ggcgtgaaat taggagaaaa actttcttct gtaagtacag ctacagcaaa
    10441 ttcatcagca tctggaaaag accgtgtaaa cgaacgggca caagaaatga aagcggcaca
    10501 aaaatcgaaa caaaaaccat ctaaaatcaa atcggtatta aaagacattt ctaatatctt
    10561 tgtgccaatg attcctgcat ttgtcggtac tgggatcgtg gcagggattg ccgcagtttt
    10621 agcaaactta gtcacagccg gaacacttga tgcagcaaca tggcaacaat atatcgatgt
    10681 gatgaacatc ttgaaaaacg ggatgtttag ctacctagtt atctatacag gtatcaatgc
    10741 tgcacaagtt tttggtgcaa caccaactct tggtggagtt atcggggccg ttgtgatgct
    10801 gacagggatg aatccggaag caccactgaa aaacttattt actggtgaag ctcttgcagc
    10861 tggacaaggt gggatcctag gtgtcatctt cgctgtttgg ctattgtcaa tcgttgagaa
    10921 aaaactgcat aaagtagtac ctgatgcggt agatatcatc gttacaccaa ctctttcatt
    10981 gattgccatc ggtctgattg aaattttctt catcatgcct ttagcaggtt tcatttctga
    11041 tggcatggtc ggtgggatca actgggtact tggcgttggt ggagcatttg ccggcttcgt
    11101 tctaggcaca ttattcttgc cgatggttat gttcggactg catcaaattt tgactccaat
    11161 ccatgtgcaa atgattgatg aaactggtcg tacgctattg cttccaattc ttgcgatggc
    11221 tggtgcagga caagtgggtg cagcattggc attatggatt cgctgcaaaa aagataaaga
    11281 attaacagaa atgatcaaag gagctttacc agtcgggatc ctaggaatcg gcgaaccatt
    11341 gatttacgga gtaacattac ctctaggacg cccatttatt actgcttgta tcggtggagg
    11401 tatcggcggg gcagtgatcg gaatgatcgg aaatgtcggg gctatcgcta ttggaccttc
    11461 aggagccgct ttgatcccat tgatctctga cggtaaatgg ttaggctaca tccttggatt
    11521 attggcagct tatgctggtg gcttcgttgc tacattcttc ttcggtattc ctaaagaccg
    11581 tttagaaaaa gaagaattag cgactgaaac agccacagta aatgaagtgc ctgaaaaagt
    11641 agtacctcaa acttcagctg aaagtgtgac tatcctctca tcagttgctg acggaacagt
    11701 tgtttcttta gataaagcaa gtgatccagt ttttgcacaa aagatgatgg gtgaaggcta
    11761 ctttgtagaa ccagaaaacg gacatatcta ttctccagtt actggggtag tcagctctgt
    11821 cttcccaacg aaacacgcta ttgggatcac aacgccaaat ggtttagaaa tcttgcttca
    11881 tatgggaatc aacacagttg acttaggcgg tacgccattt gatgtgaaag taacagaagg
    11941 gcagcaagtg acacctgata cgcttgtagc agaagctgac ttagaagcga tcaaagcagc
    12001 aggcaaagaa acatccatga tcgtcttggt aaccaatatg gatcgtgtaa aaaactttgt
    12061 cttggaaaaa acaggtaaag caaaagcaaa agcacctgtg atggatgtca aagcttaata
    12121 tgatagaaac aatagaaacg aaagaagaaa ctgaaatcta tttttcagct tcttcttttt
    12181 tattatttat tgttttctgc taaaatctga taaattcgtg tcacatcaga tggtgttccc
    12241 cgtaattcat aatctgccaa gaaaggaaag ccaaatgttt gtgcatattg tttggctgtt
    12301 agacaatact gattattgaa gtttttattt ccgctaccga ctacaccaag acaaagttta
    12361 tgattgtctt catgttccag atattcacgc atcgtttcgg tcaagatttc tgtatcaccg
    12421 ttatctaaac catttccgcc gtctaaataa gtaggaacga aagtaaaaaa tggttctgtt
    12481 tctttcgtaa aatcactatt ttcatggatt tcttttaaag taacttcagg attgacagga
    12541 tcttctgcat gcattttttc agcatattct gctagatgtt tggcaaaggc acgcgtattt
    12601 cctgaaatcg atatatataa aatgttcatg gatcatcctc caattctttc cttttagtat
    12661 agcatctttt ttaagttttt tggtggtgta atttggaaag ggactaggcg ctttgcttgt
    12721 ataatgagaa aaaagccggt agaattatat ttctattaaa gtttttatta attaagtaaa
    12781 aataaaaaag gtttgttata ctttttgata aaagcggttt tattttggag gatgcttaca
    12841 tggatacaga gcaacagaaa aaaggaattg gtttaattcc acttgctgcc ttagtaattg
    12901 gttcagcgat tggaggtggc gtatttggta ttatgacgga catctccgga tcggctggcg
    12961 gcccagctat tttcagttgg gtactcgtag gaacagcaat gttgatgctc tccttgtcga
    13021 tcaataatat caataaaaaa cgtccagatt taaaaggcgg gatctttagt tacgcagaag
    13081 aaggttttgg ccgtttcgct ggttttatca gcggttgggg ttactggctc acatgctggt
    13141 taggcaacgt agcttttgca acattgttga tgagcgcatt aggatatttc ttcccagtat
    13201 ttgaaggtgg gcaaaatctg gcatcgatcg tgatgagtac agtcttttta tggggatatg
    13261 catggctagt gaatcgggga gtcgaaaatg ctagtttcgt caatgctatc gtcacattct
    13321 gtaaattagt gccattattt ttatttattg tagtagctat cagcacgttt tcgatccaac
    13381 aattcattga taacttttct agcgacatta tattgaatgc gaatggacag ccgatcccgt
    13441 tttccagtca agtattgagt tgcatcatgg tgatgctttg ggtatttgtt ggtattgaag
    13501 gagcctctgt cgtaggttca cgagcaaaaa aacaatcaga tgttgggaaa gcaacgattc
    13561 tagggatatt cggcttgatt attatttacg tcttggtttc catgcttcca tatgggacaa
    13621 tgacgatgaa tcagctgcaa acgatcaagg cgcagcctgc tatgggctat gtgtttgaaa
    13681 tgatggttgg taaatgggga gcagtcgtga tcaacggcgg tttgatcatt tctttagttg
    13741 gtgtttggct ctcatggacg atcttaccga ttgaaacaat gggaaacatg gctgaagacg
    13801 gacttttgcc taaagcatgg ggaaaactca acaaaaatgg ggcaccaact tatgctatcg
    13861 tattgacgac gttatgtacg aatgtctttt tactttcatt attattcact gacagtgctt
    13921 ataactttgc ttacacactt ggtacagcag ctattttctt cacgtggctt ttcttaggat
    13981 tgtaccaaat gaaactttct tatcagcgga aagaatgggt ccaattttta gtagggcttt
    14041 tagcaagtgc ctttcaagta tgggctatgc tagtcgtagc ctttaatgaa gtaatgcttg
    14101 ttcttgttct tttcttgcca ggcatttatt tttacctcaa agcacgtaag gagcaaaacg
    14161 agcctgtaca acatatccac ttagataaga ttttacttat aggtgtgacg atggtcggag
    14221 tgattgcaat ggttttattt gccactggga tgcttcacgt atagaagtat aaaagaagta
    14281 taaagagagg ttgtgagcct gtcgtttatt caaaaacata ctaggaaagg agctgtgaca
    14341 caaatgttgt cacagctcct ttttcagaat aaacggtgtt taagagacag ccaatcggca
    14401 ataaggctga acgtctcttt cacagcctct tttttgatga gtcatcatac attttttcgc
    14461 ttaaatacga gatatcctaa tgctaagaac aaaacgatgt aagctaaatt accgaatagc
    14521 atttgattag tagaaagatg agtcaattgc ttcatgatct cttcgtttcc aatttggttt
    14581 tgcagattca acatactgat cggattccat ttgatccact cccatttttg gatgagtgca
    14641 ataaggatac cagatatgat tgacgaagca aaataaaaga cgattcctgc tgagatggat
    14701 gcgccggaac tattgataaa gcaagcaatc atcaacacta aacttaaaat cagccataaa
    14761 ccgatataac tacccaaagt ataagtgaac aagctttgga tcagtgtttg accagtagaa
    14821 accttctccg tgaaggaaat cgaagggaaa agaatcagtt tcatcaacac agtgacaata
    14881 atagctaaaa ggtataaata aacagaataa ataactaatg tgatccattt gcttactaat
    14941 atttctcctc gagaatactt tcgataaagg agatttttca atgtaccata ttgtgcttcc
    15001 atcgaaatga tcgtgcttgc agctgcaatc atgataaaga caatccaaga tgtggcacta
    15061 aaaagctgag cagtcatttc aactggatca atgatcgtcg tgtatttttt ggctagaagt
    15121 gctgttccta tgagcaaaac aactaataga acagataaaa cagcagttga ctttttcttg
    15181 atcaatttaa ataattcttg ttttacaagc aatgtcattt ttcttcttcc ccctttactg
    15241 aatccagaat ttccaataga gaagattcca aatcattcgt cagatgctgg acgtctgtca
    15301 catggatatt ttgtgcagac aatagctgga tcacggttcc taataattct tcgttttctt
    15361 ttaaaagctt gattttatca ggatcttcca aagtatatcc agcttctgtc aagatcgttc
    15421 gagcagctag atcatttgtt gtagagataa cgatgaaatc ttgcgctgtt tggttcaatt
    15481 cagccattgt acattgtttg atgatctttc cttgattgat caggatcagc tcatcgacca
    15541 ttttttcaag ctcgctgagt aagtgactgg agatcaagaa agaggtgccc tgagtagcca
    15601 agtcggtaat gatttttcgc acatctcttg ttgcttgtgg atccaaaccg ttcatcggct
    15661 catctaaaat aacgatagac ggatggttca gtaaggcaag agcaattccc aatttttgtt
    15721 tcattcccaa cgagtaggat ttcgcttttt tgtgaatgta cttttccatc ttgagctgat
    15781 tcacgatagt gtcgatagca gatacatcgt tggtttcaga gaataacttc agatgatcat
    15841 agcctgttaa gaatggatag atacccgggt attcgatcaa tgctcctact tgttccaacc
    15901 ctttatgact agagggagaa atctcatttt caccaataca gattgttcca tctgagtaat
    15961 ggatcagtcc taaaatagct ttcatgatcg ttgtttttcc agcgccatta ggacctacta
    16021 atccaacgat tttgccaggg gataagctaa aactgacatc gctgagggct tgtttgtgac
    16081 caaagttctt ggataattct tggaccgaaa gacttttcaa aaatattcac tccttttcta
    16141 tagatagtac aagcatacac ctgatgagtg gagaagtcgc taggtcattg gacttatttc
    16201 ttattttttc ataaaaaaat tgaatattaa ataaaaaggc ttattttttc tgatcagtac
    16261 ttattccaag tgtctagtgg ccgctagtaa tcttgatgcc aatgatactg atgagtagca
    16321 tccccacaaa gaaccaagtg gaaagattca gcttatcatg aaacaagacc acaccaatta
    16381 tgaccgaacc aactgcgcca atccctgtcc agataggata agagagactc atcggcaaac
    16441 ttttgattgc ctttgacaaa aagtaaaaac tagcaatcat gccaatcact gtgtaaaaag
    16501 aataattgat tttagaaaaa ccatcactcc atttcatcat cgttgaccag aatacttcca
    16561 aaattcctgc tatgattaat tccatccatg ccatacttac attcctcctt aactataaaa
    16621 aaatacacaa aaagaggaaa atcatccctt caaagaccta atggaatgat catcctcttt
    16681 atcctacccg gtggcagatt gtatatcttt ttctgtagta tagcacacct atccagatat
    16741 cggcaatctc atacttttta gtaaagtgta ccaagcactg acggatatat cgttagcgga
    16801 aaagaagatg aatgctttgc aatttcatag aaagaagttg ttatttatta atcatttaaa
    16861 aaaaacagta actagacctt atttctatat attatcgggc aataaattaa aaaaagcttt
    16921 accttacaat aagtaagata atttttgtga taaaaaaata acgaaaaata tttaaaaata
    16981 gtgtctattt cgtgagaaaa caactatatt ttttatgtgt tttgctttat aattaaaaaa
    17041 atattgtcat tttatggagg aaaatatatg gaaaattatc ttagcctttt aggcgtcttg
    17101 attgttattc tcgggtttgc atttaaatta gactcgatat tgatcgtaat ggttgcttta
    17161 gttgttacag ctttgactgg cggtcttggc attcaaggaa tgttagaact tttaggtaca
    17221 agttttgtaa acaatcgcgg gatggcgatt tttatcatca ttatgttggc aacaggaacg
    17281 cttgaaagaa atggtctgaa agaatcagcg gcttccttga tcaagcgatt caagaaagta
    17341 tctgctggta tgattgtaga tatttatggg atctttcgaa tgatctttgc ggcttttaac
    17401 gtgagttttg gtggagttgc tggatttgtt cgtccaatca ttttaccgat ggcattgggg
    17461 accatcgaat caaaagggtt agaagtccat cctaagcatg aagaagaact aaaaggtatg
    17521 gcttcagcta tggaaaatgt ctgctggttc tttggccaag tactattcat cggaggagca
    17581 ggggcgttgc tagtccaatc aacactgaaa gatctaggat atgacgtaac attgggacag
    17641 cttgctttag tggaagttcc agtggctatc acggctttag ttgtggcaag tgtctacttc
    17701 tatcttaaag aaaagaaatt agcgaagaaa tattatccag gaaaggggca ataagatgga
    17761 cttttttaca aatcctgata ttttattagg cgataaatta ctggagatcc tttatatcgt
    17821 gatgggactc gtattgattt acactggagt aagaaattta acagataaaa ccaatcctca
    17881 tcgttatggg tcagcctttt tctggacggt cttaggggta gttattgcag gaggaagatg
    17941 gcttccggca atcgtgaacg gtggcttgat ctttgcgatg accataccag caattgctaa
    18001 gaaagtcagc aaaggagaaa gccggcttcc atcaaaagaa tatatggaaa aaatgtcaga
    18061 caaactagga atgaaaattt ttattccagc tttgagtatc ggtgtctttg cgatcttgtt
    18121 tgctctattt accaatttag gtgctttagt cggcgtgggc gtcggtgttt ttgcagccat
    18181 gctaatcttg atgttctttt ctcgagataa tcgaccaagt acatttttag atgatgcagc
    18241 agatatgctg ggcacagtag ggccgttaag catgttgcca atgcttttag cttctctagg
    18301 tgcggtattt acaagcgctg gagttgggac agtgatttcc aatgcagtag gtacgatcgt
    18361 tccagaagga aatgtgaaca tcggcatcat catttacgct cttggcatgg tgatatttac
    18421 ggtaatcatg gggaatgcat ttgcggccat caccgtgatg acggttggga tcggtgcacc
    18481 attcgtattt agtcatggag cgaatccagc gttaatagga atggttgcat tgacttgcgg
    18541 tttttgtggg accctgctga caccgatggc tgctaacttc aatatcgtgc ctgtagcgat
    18601 gctggagatg aaagacaaat acggagtgat caaaaaccag ttgtttattg cgttatttat
    18661 gctagttttc caaattgctt atatgattct atttaaataa tagaagagag gggttgtgac
    18721 agaataattg tcgcaacctc ttctaacatt gtagtggaac actatttttt tataagaata
    18781 tattaagttt gatctgttat gttatttaaa ctattgtttc ctttatttac gctgttcaag
    18841 gaaaatggta tgataaaaga taaccatatg ttaaatcaat cggacggagg aacaacagat
    18901 gaaagtacta gtaacaggtt ttgatccatt tggtggggac aaagtgaatc cagcctatga
    18961 agcagtaaaa aaaatgccag atgaaatcag cggtgctgaa atcatcaaag tggaagtacc
    19021 gaccgtattc gaaaaaagca gtcaagtagt caaagaagcc atccaacagc accaaccaga
    19081 tgtcgtgatc tgtgtcggac aagcaggtgg gcggtcagca gtctcgttcg aacgtgtcgc
    19141 aatcaatcta gcagaagcaa gaatccctga taatgaagga aatcagccat tcgatacggc
    19201 tttagaagaa aacggtccag ccgcttactt tacctcattg ccaatcaaag caatgacaaa
    19261 aaatgtacaa gatcatggtc taccagcgta catctcttat acagcaggaa catttgtctg
    19321 caatgatatc atgtatcgtt tgcttcattt gatcgaaaca caattcccgg caattcgagg
    19381 tgggttcatc cacgtgccat tctcacccga tcaagtgatc gatcgtccag tgggtacgcc
    19441 ttctatgtct ttagaagaca ttgcttcttc tttgacttat gcagttgaag cagcagtaga
    19501 aaataaagaa gatatttccg gaaatgccgg aacaactcac taatttagca agaaacactg
    19561 gctggttgct aacgttatgt tagtagccag ctttttgtaa ttagaaagga aagaaacagc
    19621 cgtggaagaa tggcaatccg tctttgaaga atggtttcca aaagaaatca gcaaaagcta
    19681 tcccatcaaa atttctaaac aatacacaag cagtcaacgt tgggagatct atgcaaaatt
    19741 aacgaaaaaa cagcgggaac tagtcgataa gcaccgccga tatctaatca gctctcgttt
    19801 tatggaagaa cattatttag ctgcaacaga ttgggtgttt tctgacttca agatcaatcc
    19861 gttttttcga acaaaacgaa gccagcaaaa attgtattgt gagtgtggta gagaattaaa
    19921 agtgcaatac atcgttaaat cgccaaaaac cggaaagatc ctaaaactag gcatcaacca
    19981 ctttgccgat cacctgcatg tctctccgac agtggcagct tcgatccatc agggaatgac
    20041 aaaagttgat ctggccttag acgaattatt atggcttaag cagaagaata tcgattttcc
    20101 tgaaggacta tggcaaaaat actgttttgt cttgtaccaa aatcgtcgga tgaaacagcc
    20161 atatttaccg gatataaaat tagctcagcg gctcgcagaa tttcgccaag tagaaatgcc
    20221 gatttacata gcggattatc aagcattgga aaatgaaatc aagaaaataa gtgaacacat
    20281 caatgggcag tcaaaaaagc gacagataaa aaaagaacta tttgacgact ttgcagaaga
    20341 attagtaaag gatgtagaag aattcttgat caactatcgc gcatttttac gtaaagattg
    20401 gcaatccatc gtatatgaag aagtgccagt acatcctaat gcttattttg aaacatttat
    20461 ttccgtgttg agaaagacaa aacgtcaacg aacaccagaa gtcactgctc agatggaata
    20521 ttttgcgaaa aaccaacgat tcatccaacc aaaaatctat ctatttattt ggaagcaata
    20581 ttgtcgctat ggttttacag aagggttctt cgacagcatt cctcggatag tcagaaatgg
    20641 ttttttaaaa gtattgcgta aagaacgaga agctatacaa tctgctgata agaaagaccg
    20701 tacagtctca aaagaaaaat ggcaactagt agtgaaagat atccaatctg gaaatgtgca
    20761 agagacaatc gacaaatgga aaggaaaaca ttatcgtttt actgaagctc agaaacaagc
    20821 gttggaatat tatcagaagt tagaagaatc actgcgtttc aatgatgagg caagaaagta
    20881 tctgaaggaa ttactataaa aaa
//
LOCUS       DBNICI010000033        20896 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.033, whole genome shotgun sequence.
ACCESSION   DBNICI010000033 DBNICI010000000
VERSION     DBNICI010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.033"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(160..705)
                     /locus_tag="AC1ZCC_001006"
     CDS             complement(160..705)
                     /locus_tag="AC1ZCC_001006"
                     /inference="COORDINATES: protein motif:HMM:NF024811.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HIQ1629273.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(709..837)
                     /locus_tag="AC1ZCC_001007"
     CDS             complement(709..837)
                     /locus_tag="AC1ZCC_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HIQ1629274.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            complement(830..1921)
                     /gene="phnW"
                     /locus_tag="AC1ZCC_001008"
     CDS             complement(830..1921)
                     /gene="phnW"
                     /locus_tag="AC1ZCC_001008"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HIQ1629275.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            complement(2138..2617)
                     /locus_tag="AC1ZCC_001009"
     CDS             complement(2138..2617)
                     /locus_tag="AC1ZCC_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HIQ1629276.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            2976..3758
                     /gene="rpsB"
                     /locus_tag="AC1ZCC_001010"
     CDS             2976..3758
                     /gene="rpsB"
                     /locus_tag="AC1ZCC_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIQ1629277.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3857..4738
                     /gene="tsf"
                     /locus_tag="AC1ZCC_001011"
     CDS             3857..4738
                     /gene="tsf"
                     /locus_tag="AC1ZCC_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIQ1629278.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            4874..5596
                     /gene="pyrH"
                     /locus_tag="AC1ZCC_001012"
     CDS             4874..5596
                     /gene="pyrH"
                     /locus_tag="AC1ZCC_001012"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIQ1629279.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            5599..6156
                     /gene="frr"
                     /locus_tag="AC1ZCC_001013"
     CDS             5599..6156
                     /gene="frr"
                     /locus_tag="AC1ZCC_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIQ1629280.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            6351..7163
                     /locus_tag="AC1ZCC_001014"
     CDS             6351..7163
                     /locus_tag="AC1ZCC_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HIQ1629281.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            7160..7960
                     /locus_tag="AC1ZCC_001015"
     CDS             7160..7960
                     /locus_tag="AC1ZCC_001015"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIQ1629282.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            8121..9389
                     /gene="rseP"
                     /locus_tag="AC1ZCC_001016"
     CDS             8121..9389
                     /gene="rseP"
                     /locus_tag="AC1ZCC_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HIQ1629283.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            9457..11166
                     /locus_tag="AC1ZCC_001017"
     CDS             9457..11166
                     /locus_tag="AC1ZCC_001017"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIQ1629284.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            11378..15736
                     /locus_tag="AC1ZCC_001018"
     CDS             11378..15736
                     /locus_tag="AC1ZCC_001018"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HIQ1629285.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            15881..16354
                     /gene="rimP"
                     /locus_tag="AC1ZCC_001019"
     CDS             15881..16354
                     /gene="rimP"
                     /locus_tag="AC1ZCC_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HIQ1629286.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            16377..17552
                     /gene="nusA"
                     /locus_tag="AC1ZCC_001020"
     CDS             16377..17552
                     /gene="nusA"
                     /locus_tag="AC1ZCC_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIQ1629287.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            17574..17867
                     /gene="rnpM"
                     /locus_tag="AC1ZCC_001021"
                     /gene_synonym="ylxR"
     CDS             17574..17867
                     /gene="rnpM"
                     /locus_tag="AC1ZCC_001021"
                     /gene_synonym="ylxR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase P modulator RnpM"
                     /protein_id="HIQ1629288.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            17864..18175
                     /locus_tag="AC1ZCC_001022"
     CDS             17864..18175
                     /locus_tag="AC1ZCC_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HIQ1629289.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            18188..20494
                     /gene="infB"
                     /locus_tag="AC1ZCC_001023"
     CDS             18188..20494
                     /gene="infB"
                     /locus_tag="AC1ZCC_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIQ1629290.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            20521..20868
                     /gene="rbfA"
                     /locus_tag="AC1ZCC_001024"
     CDS             20521..20868
                     /gene="rbfA"
                     /locus_tag="AC1ZCC_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIQ1629291.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         7092 a         3750 c         4508 g         5546 t
ORIGIN      
        1 ttattacttg atacaggaca gctttttcac aacctcttat tttgtataaa agtcattctg
       61 gattcatgtc agcgtacaaa atgtattctg ttaactcttt taaattgttt acacaaatat
      121 cagcgccggc ttgttggaat ctaagagcaa cagactgttt taaacgatct ttttccccat
      181 ctctaatttt tggtactcat cataagaaat ccccattagc gaactacctt caattacacc
      241 tactgtcaac actccggcat ttttcccttc ctggatatct gagaccgtat ctccgacttt
      301 gattacttca tcaaccgact tgtttttgaa gtactgcatg tttttatgga tcatataagg
      361 aaacggtcga ccataccctt cgacgtcttc aaaagttgcc atgaagtctg gtgaatacct
      421 ttcttttttt gcggtttctg ctacgattgt catcatagga gcattaaatc cattcgtgga
      481 accaatattg atcccagcgt ttctcaaccg ttcgatcgtt tccaatgtgt cgggtttcaa
      541 ggtagaatgc ttgaccaaat ctttaaacag ataatgttca aattgttgat acaattcctc
      601 cacctctttt tcctgaggta gttgtccata aatagctatc cattgttttt tgacagaagg
      661 gagggaaaga agctgttgga tatgatccca ttttaatttt cccatcggtt agcggatttc
      721 tttgtcagtc agttggattc ctttttctag aaaagcattt ctaaaagcgt ggacaggagc
      781 catacagcca aagtccacag ttgttcctgc ccaatcaaaa attacagttt taatcattaa
      841 cgttctcctt atagtctttg ataattgctg tcagttccaa aatatcttcc ggatagatct
      901 cgccaatatt tccgatacga aaacattcat gatcgctgat tttcccggga tagatcacaa
      961 atccattttg ctttaaatac tgataaaaat gattaaaatc aaatcgccag tccttagggt
     1021 aaagaaaaga ggtgatgaat ggcccctgat attcttgaat gaaaggctgg aaccccatct
     1081 ctgtcatttt ttgtcgtagc acatgattat tggcgagata acgtgaatat cgagtttcga
     1141 ttcctccttc tgcctctagt tccttcagcg cttgttgaaa agccaaaacc gtatgggtag
     1201 gggaagtgaa acgccatttg ccattctcgt tttccatcac gcggtactgg tcatatagat
     1261 caagtgacaa acttcggcta acattttttc gtttttccag ttccgcttta ttagcaagta
     1321 caaaagaaaa gcctggaaca ccttgaatac atttgtttga gctggaaatc aaaaaatcaa
     1381 tccctagttc ggctacacaa atcggtatcc caccaaagct tgacatcgcg tccactataa
     1441 acaaaagatc tctttcttgt gtgacagcag caatctcttc caaattattc aaaataccgg
     1501 aagtagtctc gctatggacc attgctagtg cggagataga cggattctct tcaatcagct
     1561 gtgtaatcac atgagcttgg aggatttgat gttccgatac ctcataaacg gtatagttca
     1621 gtttcagaca ttcgcacatg ttgatcattc gttgtctata tgcgccgttt gaacaaatca
     1681 gtacagtatc ttctggcctg atcacagaac ttaataccga ttctaccccg aaggagctgc
     1741 taccttgcat taagaccgtt gtgtatattc cttccgatac ttgagcaaga tccaataatt
     1801 tttttcttat ccattgtgta atttctttgt attcttgatc ccaagtacaa tgatcgatca
     1861 gcatagcatt tttcactgtt tcagaagtcg tcaatggtcc agaagttaat aatttataca
     1921 tatgatttcc tttcatttct tttttgaaat acacaaagag caagaagctg ctcttgttaa
     1981 catccaatat aaatgatgat tattgtgatt caatcaaggt gttgaaaaat ttgaataaaa
     2041 aatgatgtat ttttgtaaag agtgtaaagg tttattcaat aaggaaaata ttgaaaataa
     2101 aaaaacacgc attccatatt aggaatacgt gctctgatta aaaaagtgat acttcagttg
     2161 gatggtgaag aaattcttct actcgttttg cgtcctcctc attttccgag atgaccatga
     2221 tcgtatcgaa tccagcaatt gtactgacaa tagagggagg tttgatctca tctaaaacag
     2281 atgcaaataa atgagcattg tctggcaagg tattgacgat cgtcaaaaag tggactcgtt
     2341 cgactttcaa tacaatgtct tcaatcattt gaaccagccg atcctcttcc tcagtcctag
     2401 tctcaggagt tcctttttcg cgaaacaata cgaagcggga aacgccattt tcatcaggtg
     2461 ccttcacgat ttttagatca cggatatctc gagaaatcgt tgcctgcgtg gcggtaacgc
     2521 cataatgccc aagtttgttt aatagttctt cttgcgtacg gatcgggaat tcttcaatga
     2581 cttgtttaat cagcaaatgt ctttgagctt tgcgcatttt ctttctcctt tacgaaagat
     2641 ttcacatgta tcataacaaa aaaaagaagg attgcccaat aatattcgtc ttttatacct
     2701 aaaattattt tatggaaaaa ggataataaa gaaacaaaga gaaatctact gtttttcttg
     2761 aattcttttt atctttcagg taaactattg aatgttgagc agaaaagtat atttgctctt
     2821 gcgatacgga tcttttgtct gtataatagc tgagggtgct ttatgcatca gcaaatccac
     2881 attcttattg gattgtcggt aggtgcttga aaaagtttgc cggcaaggta gaagataaga
     2941 agaggaaaaa taaaaccaaa tcggaggaaa caaacatggc agtaatttct atgaaacaat
     3001 tactagaagc cggcgtacac tttggtcacc aaactcgtcg ctggaatcca aaaatgaaga
     3061 aatatatctt cacagaaaga aacggaatct acatcattga cttgcaaaaa acagttaaat
     3121 tagtagatgc agcttatgat tacatgaaga gcgttgctga agaaggcggc gtagcattat
     3181 tcgtaggtac aaaaaaacaa gcacaagaag caatcaaaga agaagcaact cgtgcaggac
     3241 aatacttcgt taaccatcgt tggttaggcg gaacattgac aaactgggat actatccaaa
     3301 aacgtatcag ccgtttgaaa caaatcaatg caatggaaga agatggaact tttgaagttc
     3361 tacctaaaaa agaagttgct ggtttgaaca aagaacgtga acgtcttgaa aaattcttag
     3421 gcggtatcgc tgatatgcca agaatcccag atgtaatgta catcgttgac ccacgtaaag
     3481 aacgtattgc tgttcaagaa gcacacaaat taaatatccc aatcgtagct atggttgata
     3541 caaactgcga tccagatgaa atcgacgtag taatcccttc aaacgacgat gcaattcgtg
     3601 ccgttaaatt gattacttca aaaatggctg atgctttcat cgaaggaaac caaggtgaag
     3661 atcaagtagt tgaagaagat ttcacagaag aaaacacagc aacatcaatc gaagaaatcg
     3721 tagatgttgt tgaaggcgac aattcttcag cagaataatt ttgaatgatg gagctgtttc
     3781 aaaggctggg cgacaagcct cactctcctt tgaagcagct ttttttaaaa agaaaaacac
     3841 acaggaggag ttaaacatgg cagacgttac agctaaaatg gtaaaagaac tacgcgaaat
     3901 gactggcgta ggaatgatgg atgcgaaaaa agcattagtt gaagtagaag gcgacatgga
     3961 aaaagcagtt gatcttttac gtgaaaaagg aatggcaaag gctgcgaaga aaaatgaccg
     4021 cattgcagct gaaggattgg cttcagttgc tgtgaaaggt aacactgcag caatcgttga
     4081 agtaaactca gaaacagact ttgtttctaa aaacgaaatg ttccaagatt tagtaaaaga
     4141 aattgcagaa cttgttgctg aaaacaaacc agcagacatg gaagctgcaa tgaaaatcaa
     4201 aacagataaa ggtacaattg aatctgactt gatcgaagca actcaagtta tcggagaaaa
     4261 aatcagcttc cgtcgctttg aagttgtaga aaaagacgac aatgctgcat ttggtggata
     4321 cttgcatatg ggcggacgta ttgccgtatt gactgttcta gatggaacaa ctgacgaaac
     4381 agttgcaaga gacgtagcta tgcacgtagc agctatcaat cctcgttatg taaacgaatc
     4441 acaaattcca gaagctgaat tagaacatga aaaaactgtt cttacagaac aagcattgaa
     4501 cgaaggcaaa ccagcgaata tcgttgaaaa aatggtagaa ggacgcttga agaaattcaa
     4561 agcagaaatc gctttggtgg accaaccatt cgttaaagat cctgatatga ctgttgaaaa
     4621 atacgttgca tcaaaaggcg caactgttaa aacattcgta cgtttcgaag ttggcgaagg
     4681 tatcgaaaaa cgcgaagata actttgtaga agaagttatg agccaagtga aaaaataatt
     4741 ctgttgaata acagacggga acgcacggtt tgtgcgctcc ctttttttaa gcaaaacaag
     4801 aaaaaccagt cttagagtgt cgaaatcatc taagtatggt aaaatggcaa tagaaaatat
     4861 tggaggaatg aacatggtca caccgaaata tcagcgtgta gtcttaaaat tgagtggaga
     4921 agctttagct ggggacgcag gatttggaat caaaccacca gtaatcaaag aaatcatcca
     4981 agaaatcaaa gaagttcacg agctgggcgt tgaaatggca atcgttgtgg gcggcggaaa
     5041 tatctggcgc ggacaaatcg gagcacaaat gggcatggaa cgtgctcaag ctgattacat
     5101 gggtatgtta gctacagtta tgaatgcctt agctttacag gatacgttgg aaaacgtggg
     5161 tgtaccgaca cgcgtgcaaa cttcgattga aatgcgtcaa atcgcagagc catatattcg
     5221 ccgtaaagca gaacgtcatt tagaaaaagg acggatcgtt atctttgcag gcggtaccgg
     5281 caacccgtac ttctcgacag acacaacagc agcattacgc gcggcagaaa tcggcgcaga
     5341 tgttatcctg atggctaaaa acaatgtaga cggtgtttat tctgctgatc cgaaagtaga
     5401 tgcaaatgca gtaaaatttg aagaactgac tcatctggaa gttattgcca aaggattaca
     5461 agtcatggat tcaactgcaa gttcactaag catggataat gatattccac tgcttgtctt
     5521 caatttaaat gaacacggaa atattcgccg tgcaatctta ggcgaaaata tcggaacaac
     5581 tgtaaggggg aaataaaaat ggcagatgca attatgacag aagcaaaaga aaaaatgaaa
     5641 aaagcagcac aaaatcttca acgtgaactt gggcaaatcc gcgcaggccg tgcgaacgct
     5701 agccttttgg accgtattac tgtgaactac tacggtgcgc caacaccatt gaaccaaatg
     5761 gcatcaatcc aaattccaga agcacgtgtt ttaatgatca caccttttga caagagcatt
     5821 cttcaagatg ttgaaaaagc tattatggca agtgatatcg gaatcagtcc aaccaatgat
     5881 ggaaatgtca ttcgcttagt tatcccgcaa ctgacagaag aacgcagaaa agaattagct
     5941 aaagatgtta aaaaagaagc agaaaatgca aaaatcgcga ttcgtaacat ccgtcgtgat
     6001 gcgatcgatg aatataaaaa acaacaaaaa aatggtgata ttaccgaaga tgatttgcgc
     6061 ggattagaaa aagacgtaca aacattgaca gataacagca tcaaagaagt agataagatc
     6121 gctgctgata aagaaaaaga attattggaa gtttaataaa atcataaaaa cagaggggcg
     6181 gatcattccg cttcctctgt ttttatttat atttctttta tttaaaaatt aaaaaaagaa
     6241 aaaattattc agtttttaaa taaaaaaaca atgatttcat aggaaaaaca gttagatatt
     6301 gctataatac ttaagatagt gggaatcttt taagtatagg aggaaaaaga atgttacgtt
     6361 tttttccgca aaaaaacaaa tatattcaag aaaaaagcga atttcaattt aataccgatg
     6421 gaccaattcc tcagcacatt gccatcatca tggacggcaa tggtcgttgg gcgcaaaacc
     6481 gtcgtttgcc acgagttgct ggccataaag aaggcatgga aacagtaaag aaagtcacaa
     6541 aaaaagcatc aaggcttgga gtgaaagtgt tgacgcttta tgccttttcg actgaaaact
     6601 ggaagcgacc aaaagatgaa gtaagttttc tcatgcaact acctgtcgac ttctttgata
     6661 cgttcgtacc agaattgatt aaagaaaatg tcaaagttca tgtcatgggt tatgaaaacg
     6721 tattgccaga acatacgcaa gatgctgtaa gacgtgcaat tgagcaaaca aaaaacaata
     6781 ctggaatggt attgaatttt gctttgaatt atggaagcag agcagagatc gtgactgctg
     6841 tccaagaaat tgccgaagaa gtagcaaaag gagaaataca cgcagaagaa atcgatgatg
     6901 aattgattgc taagcattta atgacaggtt tcttgcctaa agagttacaa gatcctgagc
     6961 tgatgatccg tacaagtgga gaagagcgaa ttagtaattt tctgttatgg cagatcgcat
     7021 atagtgagct gtattttaca aaagcgcttt ggcctgattt tgatggtgcg catcttgaag
     7081 aagcaatcgc ctcttatcaa aatcgagacc gtcgattcgg cggtgtaaaa aaaacagaag
     7141 atgagaaagg ggatcagtca tgaaacaacg tgtggtcaca gcagccgtgg cgctagtctt
     7201 gtttattccg attatatgga tcggaggcat agcggttgag cttacagcag caatcttagc
     7261 agtaatagga gtttacgagc tgtttcggat gaaaggattg acactcttaa gttttgaagg
     7321 agttttatcc gccgttggtg cagtgttact tgttttgcca aaagaaagat ggtttttctt
     7381 tcttccagag aatgtaagta catttatgtt attttatctt atagttatga ttcttttagg
     7441 aacatcagtt atttctaaaa atacgtatac gattgatgaa gcaggttttc ctgtgcttgt
     7501 cagcttgtat gtgggaatag gtttccagaa ttttgtcaat gcacgaacag aaagcttggc
     7561 tgttttgata tttggtctat ttattgtttg ggcaactgat atcggagcat atatggttgg
     7621 tcgacgttat ggtcagcata agttaagtcc tgatatttcg cccaacaaaa cgattgaagg
     7681 tgcgctcggt ggtattggta gtgctgttgt agtcgcattt ctttatttat tgctataccc
     7741 agcaaaagaa ctatttaatc atggtatgtt tgtcatgctt ctgatgacga ttattttttc
     7801 agcagtcgga caatttggtg atttggtcga atctgcaatc aaacgtcatt atgatgtgaa
     7861 agactcggga aatatcttac caggccatgg tggaatctta gaccgtttcg acagtttgct
     7921 gtttgtattt cctatcatgc atttatttgg cattatttag taagtatacc tgcgtttctt
     7981 tgtgaatttc ataaaagacg caggttttta tttttcatta tattgatact attggtgtct
     8041 ttggtatact atggtaaaat tatagtgttt tatcgttgtt ttataaaaaa cacaaaaaac
     8101 aagtaaagga gaaaaaagct atgaaaacga ttctgacatt tatcatcgtt tttggtatat
     8161 tagtgattgt tcatgagttt ggtcatttct tctttgcgaa acgatcagga atcctcgttc
     8221 gcgaatttgc tatcggtatg ggtccgaaaa tttatgggca tcaagcaaaa gacgggacaa
     8281 catatacgct tcgcctatta cctattggcg gttatgttcg aatggcaggc aatggggatg
     8341 atgaaacaga aatggcacca ggaatgcctc tttctctttt actaaattct gacggcatcg
     8401 tagagaagat aaatttaagt aagaaaatcc aattgacaaa tgctatcccg atggaattga
     8461 gccgttatga tttagaagat gagctgacta tcactggtta tgtgaatgga gacgaaacag
     8521 aagtagtaac ctaccctgtt gatcacgatg cgacgattat tgaaaacgac ggaacagaaa
     8581 tcagaattgc gccaaaagac gtacagttcc aatcggctaa attgtggcag cgtatgctga
     8641 ccaactttgc tggacccatg aataacttta ttttggcaat cctgttattc atcattctgg
     8701 catttatgca aggtggtgtt caagtgacga acactaatcg cgtaggagag atcatgccta
     8761 atggagctgc ggcagaagca ggcttaaaag aaaatgatga ggtagtcagt gtggacggaa
     8821 aagaaatcca ttcatggaat gatctgacaa cagtcattac gaaaaatccg gataaaacat
     8881 tagactttaa gatcgaacga gaaggtcagg tgcaatctgt cgacgtgaca ccgaaatctg
     8941 tagagtcaaa tggcgaaaaa gttggccaac ttggtataaa agccccaatg aatacaggtt
     9001 ttatggataa gatcattgga gggacgcgtc aagctttcag cggttcattg gaaattttca
     9061 aagccttagg atcactattt acaggtttca gtttagataa attaggcgga cctgtcatga
     9121 tgtaccaatt gtcttcagaa gcagcaaatc aaggtgtaac aactgttatt agtttgatgg
     9181 cgcttctttc aatgaatctc ggaatcgtca atctgcttcc gattcctgcc ttagatggcg
     9241 ggaaattagt cttgaatatt tttgaaggga ttcgcgggaa accattaagt caagaaaaag
     9301 aaggaatatt gacattagct ggctttggat ttttaatgtt gttgatggtg ctagttacat
     9361 ggaacgatat tcaacgattc tttttctaga aaaagttgac aaatagggta taataacaaa
     9421 cgataactat tttgaggaaa taaaggagta tatagaatga gacaatcaaa aatgttgatt
     9481 ccaacattac gcgaagttcc aaatgatgca gaagtattaa gccatcaaat cttgctaaga
     9541 gctggatata tccgccaagt atcagcgggt atttattcat atctgccatt agcaaatcgt
     9601 gtattggaaa aactgaaacg cattatgcgg gaagaatttg aaaaaatcgg agcggtagaa
     9661 atgctgatgc ctgccatctt gccagcagaa ctttgggaag aatccgggcg ttacgaaaca
     9721 tatggaccaa acttgtatcg tttcaaagac cgcaatgatc gaaaaatgat ccttggacca
     9781 actcacgaag aaacttttac tgatttgatg cgtaatgaaa tcaattcata taagaaatta
     9841 cctttaaatc tttatcaaat ccaacctaaa tatcgtgacg aaaaacgacc tcgcttcggt
     9901 ttgcttcgcg gaagagagtt tatcatgaaa gatgcgtatt ctttccatgc aacggaagaa
     9961 agtttggacg agacatataa agattacgaa aaagcctaca ccgaagtatt caagcgttgt
    10021 ggcttagaat ttcgctcgat tatcggagac ggtggcgcaa tgggcgggaa agactcaaaa
    10081 gaattcatgg cgatctcaga aatcggggag gatacgatct gttattcgac tgaaagtgat
    10141 tatgctgcaa acttggagat ggctacaagc tactatgtat caaaaaaatc gcatgaaaca
    10201 cagcttgaat tggagaaagt agctacacct aacgtaaaat caattgacga agtagcagat
    10261 ttcttttctg ttgaacctca aaaaatcatc aagtctgtat tgttcattgc ggatgaacaa
    10321 cctgtccttg ttttggtacg cggagaccat gaagtcaatg acgtaaaact gaaaaacttt
    10381 ttatctgctg actttttaga agaagcaaca gaagaagaag cacaaaaata tctaggtgct
    10441 gagtttggtt ctgtcggacc agtcggtgtt tcagatgacg taaaagtata tgcagaccgt
    10501 tatgtccaag atttagcgaa tgccattact ggagcgaacg aaacaggatt ccactataaa
    10561 aatgtgaatc cagaacgcga tttcactgta ctttcttatg aagatcttcg ttttgtacaa
    10621 gaaggagatc cttcaccaga caacaatggt gttcttgcct ttactcgagg aatcgaaatc
    10681 ggacatatct tcaaaatcgg cactcgttac agtgaatcta tgggagcaac tgtactagac
    10741 gaaaatggac gagaaaaatt tgtgattatg ggctgttatg gtatcggtgt aagtcgttta
    10801 ttatcagcaa tcgtagaaca acattcagat gaacaaggaa tcaactggcc aaaaggaatt
    10861 gcgccatttg atttgcatat cgtccaaatg aatctaaaag acgaatacca aacaaacctg
    10921 acgaatgaac tagaagaagc gatgacaaaa gctggctatg aagtactggt tgatgacaga
    10981 aatgaacgcg caggagtgaa attcgcggat tctgatttga ttggctgtcc aatcagaatc
    11041 actgtcggta aaaaagcagt ggatggaatc gtagaagtga aaatcaagaa aactggcgaa
    11101 atggttgaag tacgtaaaga agaattggtg aatactttac caatcctctt gaaccaaggc
    11161 gaataataag cgttaaaaca cagaaaataa aaatagctga atcagctgtt tttgacaatc
    11221 tgatgattcg cttttaaaat tggaactgcg atttgaatcg cagttccaat tttttaatgt
    11281 aaaactcaga aaaaacgagt atactaaaag gtactgaaaa agaagaggat aagatgcgat
    11341 caattgcatt gtttctcatc agaaagggcg tatatctttg tcagaaaaac gagatttatt
    11401 tgaaaaactg ttggaacaaa tccaactaga ggaagcagaa aaaaatcatc cactgcttac
    11461 tgctggtgaa atggatagtg tagtggtcca tcgtaaatcg cgtttatggg aatttacgct
    11521 gcatttttcc aaaattttgc ctattatgct atatcggtca ttgactcagc atctgactat
    11581 cgcttttaaa gatattgcac aagtcaaact gaatatctta gcagatgacc agacattcga
    11641 tgaacagctc ttgcaagatt actgggccca agctttggaa aatcagcaat gcgatacacc
    11701 actagcacag caagtattga aaacgcaggt acctgtgata aaagatcgta aagtcatctt
    11761 gccaatcgac agtacaggag ctatttctta tttgaagcaa caatacttgc cattagtaga
    11821 agagttgttc gtcagttacg gattcccaaa atttcgtatc gaaccagaag tggatgagca
    11881 gcaagcggaa cgagtattga aactttttga agaacgcaaa caagaacagg cagaagcctt
    11941 tatgaagcaa gcagctgaaa gtctagttgt ccacgaacaa aagaaaaagg aaagaaaaga
    12001 gcaatcgcca gctcttgaag gacccatcca catccagcta ggacgaaata ttccagccga
    12061 tgaaccaacg acaccaatga tcagtatcgt tgaagaagaa cgaagagtca cacttgaggg
    12121 gtacgtattc gacaaagaag tcagagaact acgttcaaaa cgcaagatct tgactttgaa
    12181 aatcacagat tatacttcat catttatcgt caaaaaattc tcgaacggcg aaaaagatga
    12241 acaagtattt gatgcgatca gtgtcggtag ctggttaaag gtacgaggaa gtattcaaga
    12301 agatacgttt gtccgtgatt tggtcatgaa tgcgcaagat atcatagaag tcaaacatac
    12361 acctcgcaaa gattatgctc ctgaagggga aaaacgagta gagcttcatg ttcatagcaa
    12421 tatgagtacg atggatgcaa caaacagcat ctctgatctt gtggcacaag cagggaaatg
    12481 gggacaccga gctatcgcca ttacagacca cggtggtgcg caagcctttc cggaagcaca
    12541 tagtgctgga aaaaaagcag gtgtgaaaat cttatacggc gtggaagcga atgtcgtaga
    12601 tgatggtgtc ccaattgctt ataatgatgc acatgaagca ttgagtgaag ccacatatgt
    12661 cgtatttgac gtggaaacga caggattgtc tgcagtatat gacacgatca tcgagctagc
    12721 cgcagtcaaa atgtataaag gaaatgtgat cgaaagcttt gatgaattta tcgatccagg
    12781 acatcctttg tcacgaacaa cggtagatct gactggtatc acggatggaa tggttcgagg
    12841 atcaaagtcg gaagaagaag tattgcggat gttccttgaa ttttcaaaag atacaatttt
    12901 ggtagcccac aacgcggctt ttgatatggg gttcttgaat actagctacg caaggtatgg
    12961 tattcctgaa gcggccaatc cagtcattga tacgttggaa ttagctagat atctgtatcc
    13021 tcaattcaaa cgtttcggat taggtgtgtt atctaaaaaa tttggtgtca gcttggaaca
    13081 gcatcaccga gcaatctatg atgctgaagc aactggtcac ttagcatgga tctttgtgaa
    13141 agaggcaatg gacaatcata acatgcttta tcatgatcag ctaaatgagc atatcggaga
    13201 aggagattct tacaaaaggg ctcgaccttt ccatgtaacg attttagcaa aaaatcaggc
    13261 aggactgaaa gatttgttca aactgatttc aatgtcgaat gtcgaatact tcgaacgcgt
    13321 cccacgaata ccacgctccc aattgaaaaa gatgcgtgaa aatctattga tcggctcagc
    13381 ttgtgataag ggagaaatat tcgaagccat gatgcaaaaa ggggtagaag aggcaagaaa
    13441 ccgagcaaaa ttctacgatt atattgaagt tatgccaaaa gcggtctatg cgcctttgat
    13501 cgagcaagaa ttggtaaaaa atgaacatga tttggaagaa attatccaaa atttggtaga
    13561 aatcggaaaa agtttggata aaatcgttgt tgcaacaggt aatgtacatt atctgaacga
    13621 agaagatgcc atttaccgaa aaattttgat caattctatg ggcggagcga atccgttgaa
    13681 tcgtcatagt ttgccggatg tccatttccg aacaaccgat gagatgctaa ctgcatttca
    13741 ctttttaggg gaagaaacag cgaaagagat cgtagtagaa aatacgaata aaatcgcaga
    13801 tatctgcgaa gaagtgattc cagtcaaaga tgaactttat acaccgaaga ttccaggttc
    13861 agaagatgaa atatcagaat tgagttacac aaaagcaaaa caaatgtacg gagatccgtt
    13921 acctgagatc attcaaaaac gattaaaaaa agagttgaat tctattaatg gtaatggctt
    13981 ctcggttatt tatctgatcg cccaaaaact agtgcataaa agtaatgaag atggctatct
    14041 agtaggttca cgtggctctg ttggctctag ctttgttgcg acgatgacag gaatcactga
    14101 agttaatccg ttagcccctc attattattg tccagagtgc caatattccg agttttttga
    14161 agatggtacg tatggttctg gatttgacat gccagaaaaa caatgtccaa aatgtggtgc
    14221 acgcttaaac aaagacggtc atgatattcc atttgagaca tttttaggat tccatggaga
    14281 caaagtgccc gatattgatt tgaacttctc aggtgattat caagcagaag cccataacta
    14341 tacgaaagta ttgtttggcg aagactatgt ttaccgtgcc ggaacgattg gtacagtcgc
    14401 cgacaaaaca gcatatggct atgtaaaagg atacgaaagg gacaataatc tgcagtttcg
    14461 aagtgcagaa gtcgacagat tagcaaaagg cgctactgga gtcaaacgga caaccggcca
    14521 gcatccaggg gggatcatcg ttattcccga ttatatggat gtctatgatt ttacgccgat
    14581 ccaatatcca gcagacgatc aaaattcgga atggaaaacg actcattttg attttcactc
    14641 gattcacgat aatgtattga aattagatat tctagggcat gatgacccaa ctgttattcg
    14701 gatgcttcaa gatttatcag ggatcgatcc acaaacgatc cctacggatg acccggaagt
    14761 gatgcggata tttgccggtc ctgaagtact tggagtaagt caggaacaaa tttattccaa
    14821 aacagggaca ttaggtattc cagaattcgg cactcgtttt gttcgaggga tgctagaaga
    14881 aacgcatcct acaacttttg cagaactact gcaaatttcc ggactatctc atggtacaga
    14941 cgtatggtta ggaaatgcgg aagaattgat tcgacgagga gatgcgacac tggcggaagt
    15001 aatcggctgt cgtgatgata ttatggttta tttgatccat gctggattag atagcgggat
    15061 ggcatttaaa atcatggaaa ctgttcgtaa aggacaatgg aacaagatcc ctgatgaatt
    15121 gcgagaaacc tatctatcgg cgatgaaaga aaataatgtg cccgactggt atatcgattc
    15181 ttgttcaaaa atcaaatata tgtttccgaa agcccatgct gcagcttacg tattgatggc
    15241 tcttcgtgtc gcttatttca aggtttattt ccctattttg tattactgtg catacttttc
    15301 tgtacgtgca gacgactttg atctagtctc catgtgcaaa ggaaaagatg ctgtgaaaca
    15361 agcaatgaaa gaaatcacgg acaaaggatt agatgcttcc gtaaaagaaa agaatcaact
    15421 gacagtgctg gagttagcca atgaaatgct ggaacgagga ttcaaatttg gaatgattga
    15481 tttgtataaa tcggatgccg ttaactttgt tattgaaggt gatacgttga tcgcaccatt
    15541 tagagctgtc ccaagtttgg gaaccaacgt ggcaaaacaa atcgttgaag caagaaaaga
    15601 cggtccattt ttatccaaag aagatcttgc cacccgcggt aaagtttcaa aaacattgat
    15661 cgaatatatg aacgacaatg gcgtattgaa agatctacca gatgaaaatc aattatcgtt
    15721 attcgatatg ctataagcat ctgttttcct gtctgcttgt aattttaagc agagtatggt
    15781 atagttctta taggtaatga tactaactgt gagtgagcgg atttttcgct cactcttttt
    15841 aatggaatta cgcacttaat tttttaagga ggcgaaactt ttgagtagcg tcgttgaaac
    15901 agtcacagaa atggtgacac cgattttaga tgaacaaaat tttgaacttg tcgaagtcga
    15961 atttgtaaaa gaaggaaaaa gctggttctt acgagtattt attgacaaag aaggcggtat
    16021 cgatatcgaa gaatgcgcct ttgtcagtga gaaactgagt gaaaaacttg atgcagcaga
    16081 tccagatccg attcctcaag cttatttcct ggaggtatcc tccccgggag cagagcggcc
    16141 attgaaaaaa gaaagcgact atgaaaaggc acttggtgat tatgtccatg tctccttata
    16201 ccaaccagta gatggcgaaa aacaatatga aggatttctt caatcatttg atgctgaaca
    16261 gcttacattg aaaatacgca taaaaacacg agaaaaagaa ttgacttttg accgaaaaaa
    16321 tattgcaaaa gctcgcttag ctatccaatt ttaatcacgg agaggaatca gaaaaaatga
    16381 gtaaagaaat gttaaatgcg ttagacgctt tagaagctga aaaagggatc tcaaaagaaa
    16441 tcgtgatcga tgctttagaa gcagcattgg tttctgcata caaacgtcac tatggccaag
    16501 cacaaaacgt cgaagtggaa tttgatcaga aaaaaggaaa gatccatgtc tatgcggtaa
    16561 aagaagtaac ggaagaagtg atggattcac agttggaagt ctcattgaaa gatgcacttt
    16621 taatcaatcc agcatatgag atcggtgata cgatccgttt tgaagtaaca ccaaaagatt
    16681 ttgggcggat cgctgcgcaa acggctaaac aagttatttt acagcgtgta cgtgaagcag
    16741 aaagaacgat tatttataac gaattcagtg cttatgaaaa agacatcatg caaggaatcg
    16801 tcgaacgcca ggataaacgt tatatttatg tcaatcttgg taaaattgag gcagttcttt
    16861 ctaaacaaga tcaaatgcca aatgaatttt atcaaccaca tgatcgaatc aaagtttatg
    16921 tttcccgagt agaaaatacg tctaaaggtc cgcaagtatt tgttagccgc agccatccag
    16981 acctattgcg ccgcttattt gagcaagaag taccagaagt ctatgatgga atcgttgaaa
    17041 ttgtcagcat cgcccgtgaa gctggcgatc gttcaaaagt ggcagttcgc tcaaatgatc
    17101 caaatatcga tgcagtcggt acatgtgtcg ggccaaaggg acaacgagtt caagcaatcg
    17161 tcaatgaatt aaaaggcgaa aacatggata tcgtcgaatg gaatgaagat ccagctgtat
    17221 tcattgcaaa tgcattgaat cctgcacaag tcatggatgt gatcttcgat gagacaaatc
    17281 caaaggcctg cacagttgtc gttccagact accaattatc gctagcaatc ggtaaacgcg
    17341 gacaaaacgc acgtttagct gctaaattga ctaaccataa aatcgatatc aaatcagaat
    17401 ctgacatgac ggaattttat gaaaatcaag aacaacaaaa agaaacagaa gaactgcatg
    17461 atgaagcaat cgttcagtca gatttaacag acgatgaata tgaaacaatt gcattcaaca
    17521 atgaaacggt cgaagaaaaa ccagaagctt aactttagta aggaggcaaa gagatgaaac
    17581 aaaggaaaat tcctttacgc aaatccgtgg tttcaggtga aatgaaacct aaaaaagaga
    17641 tggtccgaat cacccgttca aaagaagggg aagtatcgat cgatccaact ggaaaaatgc
    17701 ctggacgagg agcgtatgtc tcacttgagc cggaagaagt acaacaagct tgggataaaa
    17761 aaattctcga tcgagtactc gaaacaacat taacagatga gttttatcaa gaacttcttg
    17821 actatgtcac tcaccaaaaa gcacgtaaag agttatttgg aaaatgaata cagttaatcg
    17881 gcaaaaagcc atgaatctca tcggtctagc gatgcgcgcg ggaaaactaa tcacgggtga
    17941 agaactgacc attgctgaaa tccgcaaaca aaaagcaaaa ttggtgtttg tggcaaccga
    18001 tgcaagcgag aatacaagaa aaaagataaa agataagagt tcatattatg aagttccttg
    18061 ctttgagcta ttttctgaag cagaaatcac gcagatgatc ggaaaacctc gcaaggtatt
    18121 cggaataatg gatgccggat ttgcgaagaa gaccaaggag ctaattgaag gttaggaagg
    18181 tgattgcatg ggcaacaaaa gaatctatga attagcaaaa gaactcaaaa aatctagtaa
    18241 agacgtcgtg gaaaaagcac aacaacttgg catcgatgtg aaaaaccata tgggtgcgat
    18301 tactagcaat gatgagaaga aattgcaaca agcgttcaaa aacccaagca cgaaacaaca
    18361 agctgcacaa caagcaagca gaaaaccagc caatcaacag ccgaacgatc aacgtaaaaa
    18421 agaaacaacg aacaatcaac aaaaaaataa aaataaccgc agttatcaag atcgcggtca
    18481 agggagcggt caagtgaatc aaggaaaaaa accaacaaca aaccaaaata atacgggaaa
    18541 taccagcagc caaaatcgtc aaggaacgag ccaaggcggc aatcaaaacc gtactaacaa
    18601 taacaacaaa ccaaacaata acaatcgcgg caaatttaat aataataacc gtaatcgtta
    18661 taataaaaaa ggtaaaaaag gcaaacagca acaatcaact aaaccagctg taccaccacg
    18721 caaattccgt gaattgccag atgtattaga gtatactgaa ggtatgaacg tagcagatat
    18781 tgcgaaaaaa attcatcgtg agccagcaga aatcattaaa aaattattta tgcttggtgt
    18841 catggtcaat caaaaccaag cactggataa agatacgatt gaactgttag cgacggacta
    18901 tggcatggaa ccacaagaaa aagtccaagt tgacattgct gatatcgata aattcttcga
    18961 ggcagaagaa gtcgatccag ataaattagt ctcacgtccg ccagtcgtaa cgattatggg
    19021 acacgtagac catgggaaaa caactttatt ggatacatta cgccattcac gtgtaacaag
    19081 cggcgaagcc ggcggaatca cacagcatat cggtgcctac cagatcgata ttgatggtaa
    19141 accaatcact ttcttggata caccaggaca tgcggccttt acaagtatgc gtgcacgtgg
    19201 agcaagtatc acagatatca cgatcttagt tgttgctgca gatgatggcg tcatgccaca
    19261 aacagttgaa gcaatcaacc atgccaaagc tgcaggcgtt ccaatcatcg tcgctgtaaa
    19321 taaaattgat aaaccaggtg caaaccctca acatgttatg caagaattaa gtgagtatga
    19381 gttaattcct gaagcatggg gtggagaaac gatctttgtt gagatctcag cgaaattcgg
    19441 ccaaaacatc gaagaattgc tggaaatgat cctgttagtc gcagaagtag aagatttaaa
    19501 agcagatcca acacaacgcg caatcggaac agtgatcgaa gcacgtcttg ataaaggtaa
    19561 aggtcctgtg tcaaccttac ttgtccaaga aggaacactt catgttggtg acccaatcgt
    19621 tgtcggtaat acttacggac gtgtccgtgt aatgacaaac gatcttggcc gtcgcgataa
    19681 agaagcagga cctgcaacgc cagtagaaat cactggcttg aatgatgtac ctcaagcagg
    19741 tgaccgtttc gtagtcttcg aagacgaaaa gacagcacgt gcagcgggtg aagaacgcgc
    19801 gaaacgtgca ttattagaaa accgtgctgc cacaagccgt gtgactttgg ataacttatt
    19861 tgaaagcctg aaagaaggcg aattgaaaga agttaacgta atcatcaaag cagatgtaca
    19921 aggttcagca gaagcactgg ctgcatcatt gaagaagatt gatgtggaag gtgtccgtgt
    19981 aaaaatcgtc cattcagctg tcggagcaat caatgaaagt gatgtaacct tagcagcagc
    20041 cagcaacgct attatcatcg gtttcaacgt tcgtcctaca ccgcaggcaa aagtgcaagc
    20101 tgatactgaa gaagtcgata ttcgtcttca ccgtatcatc tataaagcca tcgaagaaat
    20161 cgaaactgcg atgaaaggga tgctagaccc tgaatttgaa gaaaaaatca ccggtcaaat
    20221 gatcgttcgt gaaaccttca aagtttctaa agttggtacg attgcaggtg cctatgtaac
    20281 agaaggatat attcgtagag atagcggtgt ccgtgtaatc cgtgacggaa tcgtaatcta
    20341 tgaaggccaa cttgcaagtc tgaaacgatt caaagatgac gtgaaagaag tcaaaatggg
    20401 ttatgaatgt ggtgcaatga ttgaaaaatt caatgacatc aaagtagatg acgtaattga
    20461 aggatttatc atggaagaag tcaaaaatga ctaaaaactg ataaaaggag gccaatcatt
    20521 atggctaact accgtgaccg ccgagtcggt caagaaatct taaaagaagt caatgatatt
    20581 ttgcgcaaaa aggtccgtga ccctcgtgtg gagaacgtaa cgatcacaga tgtgaaagtt
    20641 acgggggact tacagcaagc aacgatctat tacagcattt tatctgactt agcatcagat
    20701 aagaaaacag cacaagatgg attgaataaa gcatcaggat tgattcgtcg agagttaggg
    20761 cacaatctaa gtatttataa gacacctgaa ttgttctttg aacttgatga atcagttgtc
    20821 tatggcaacc atatcgatga attgcttcgc gacttgaaca aagactaatc aaaaactccc
    20881 gcaaatcctg tgaaat
//
LOCUS       DBNICI010000034        20892 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.034, whole genome shotgun sequence.
ACCESSION   DBNICI010000034 DBNICI010000000
VERSION     DBNICI010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.034"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..189
                     /locus_tag="AC1ZCC_001025"
     CDS             <1..189
                     /locus_tag="AC1ZCC_001025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629292.1"
                     /translation="GYMVSETAKTLSLQQKITLSPFHFRYISLKRIPKKQKKKEPLVS
                     LCFLLVPLKTRSYNNGVG"
     gene            complement(341..3490)
                     /locus_tag="AC1ZCC_001026"
     CDS             complement(341..3490)
                     /locus_tag="AC1ZCC_001026"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HIQ1629293.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3504..4748)
                     /locus_tag="AC1ZCC_001027"
     CDS             complement(3504..4748)
                     /locus_tag="AC1ZCC_001027"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HIQ1629294.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIKFKLEPGDFIMSGAGTIGRIS
                     RVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPM
                     SEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAK
                     VPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRL
                     DDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFIL
                     QFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLDDTIA
                     LHQRKLDLLKETKKGFLQKMFV"
     gene            complement(4741..6333)
                     /locus_tag="AC1ZCC_001028"
     CDS             complement(4741..6333)
                     /locus_tag="AC1ZCC_001028"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HIQ1629295.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(6843..7568)
                     /locus_tag="AC1ZCC_001029"
     CDS             complement(6843..7568)
                     /locus_tag="AC1ZCC_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629296.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(7722..8048)
                     /locus_tag="AC1ZCC_001030"
     CDS             complement(7722..8048)
                     /locus_tag="AC1ZCC_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629297.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(8085..8450)
                     /locus_tag="AC1ZCC_001031"
     CDS             complement(8085..8450)
                     /locus_tag="AC1ZCC_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HIQ1629298.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(8487..8702)
                     /locus_tag="AC1ZCC_001032"
     CDS             complement(8487..8702)
                     /locus_tag="AC1ZCC_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIQ1629299.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            8831..9166
                     /locus_tag="AC1ZCC_001033"
     CDS             8831..9166
                     /locus_tag="AC1ZCC_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1629300.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(9242..11239)
                     /locus_tag="AC1ZCC_001034"
     CDS             complement(9242..11239)
                     /locus_tag="AC1ZCC_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629301.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(11337..11612)
                     /locus_tag="AC1ZCC_001035"
     CDS             complement(11337..11612)
                     /locus_tag="AC1ZCC_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1629302.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            11844..12578
                     /locus_tag="AC1ZCC_001036"
     CDS             11844..12578
                     /locus_tag="AC1ZCC_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIQ1629303.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            12617..13759
                     /locus_tag="AC1ZCC_001037"
     CDS             12617..13759
                     /locus_tag="AC1ZCC_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIQ1629304.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(14387..14872)
                     /locus_tag="AC1ZCC_001038"
     CDS             complement(14387..14872)
                     /locus_tag="AC1ZCC_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HIQ1629305.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(14874..16310)
                     /locus_tag="AC1ZCC_001039"
     CDS             complement(14874..16310)
                     /locus_tag="AC1ZCC_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HIQ1629306.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPH
                     YCIMRKILKENFVDSEDFFDQERYNYELTKRRLQINDLSRFSINVVEVDDYSEITDIL
                     KSIRKKYLRKTIFISGSAVDYTPFTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGN
                     SIVSGVLKQRRNLRKNNIQDVLSLRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLE
                     SGEIVTADGMIEEFELSVQNENIVVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQ
                     ALEKLDTEGEVDKKVEQIVSLINSIQED"
     gene            complement(16343..17194)
                     /locus_tag="AC1ZCC_001040"
     CDS             complement(16343..17194)
                     /locus_tag="AC1ZCC_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HIQ1629307.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            complement(17350..17595)
                     /locus_tag="AC1ZCC_001041"
     CDS             complement(17350..17595)
                     /locus_tag="AC1ZCC_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1629308.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            17700..18473
                     /locus_tag="AC1ZCC_001042"
     CDS             17700..18473
                     /locus_tag="AC1ZCC_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629309.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            complement(18526..18840)
                     /locus_tag="AC1ZCC_001043"
     CDS             complement(18526..18840)
                     /locus_tag="AC1ZCC_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629310.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(20359..20577)
                     /locus_tag="AC1ZCC_001044"
     CDS             complement(20359..20577)
                     /locus_tag="AC1ZCC_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629311.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
     gene            complement(20592..>20892)
                     /locus_tag="AC1ZCC_001045"
     CDS             complement(20592..>20892)
                     /locus_tag="AC1ZCC_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HIQ1629312.1"
                     /translation="RENVQSSTSSQGFKTVDVRENVQKDSGEMKEKTQKINIVEDRKS
                     AKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNELFRDTLNDPNPLFDNLLKK"
BASE COUNT         6471 a         3923 c         3001 g         7497 t
ORIGIN      
        1 ggttacatgg tatctgaaac agctaaaacc ttgtcattac agcaaaaaat cactttatct
       61 ccgtttcatt tcagatacat ttcacttaag cggattccaa aaaaacaaaa gaaaaaggaa
      121 cccctagtta gcctatgctt tttattggtt cctctcaaaa cacggtcata taacaacggg
      181 gtggggtaaa tgaatgaaaa aggtggagga aacattattc aatagctgtc ttaaatactt
      241 cgttataaaa tgtgaacaaa taaaagacat ctatccctat tttctacact tccactttac
      301 taccatcttt gaatcgtaca attttcttaa ctcttctcgt ttacctcaat tcattttcaa
      361 gtggaatcag ctcatcatct aacattttac gccatgctcg cttcatttct tgtgggtatt
      421 taaatggctt cgcttctgga tggttggctt tatatccttc gtaatcctta ctgttgataa
      481 ttgctttcat attaggaatt ggatcttcac ctggcatata ttgcagatta gaagcatgta
      541 attctttctc agaaacaaac cactcattgg aaaagacatg aatcaaatca tcactcgctt
      601 gtgtaaaata gcgacgttta atgacaaaga tatcttcatt actatctaaa gtccccacat
      661 caaacgcatt taaaatttgt cggtaaactt ctttgactcc ttctgtttta ttcaatttag
      721 ccaaagcttt ttcaatttct tctcgtgcat ctgaactatt cgccgcataa gttcctagta
      781 attgatcaat ataagtagaa tcaatatcat acaacttcgt ggtattttca ctataaaatt
      841 cgatcgtgga aaaatcttct ccctcttttt ctggttcttc ctctattaat gaacccttca
      901 cggtctcgta cacgccaatt tgttcttcca aaagttgaat ttgattgttc aaggttgaag
      961 atttcaccat gtcatcattg taatcgtcat aagtcactaa cgattcataa gcacgattca
     1021 attcttggaa tgctttgacg tactcgatac ggatttcaag tggcgtatgt tcatcaatgg
     1081 attccatgga tgaaacctct tttaacttac catatgcttg cttgaatctt tttttggatt
     1141 cctgataact tggataaatc aaacccgatt cttcttttgc ttctgaatac agttttgtcg
     1201 catcctcgac attcttctcc attgtggctg gctttcggaa agtaacaatt agtccttttg
     1261 ttttttctgg atacgtacgg ttcgtacgtg aaaaagcttg gatcaatcct gcatactcta
     1321 gattacgatc cacaaatagg gtttgaatcg taggtgcgtc aaaacctgtc aatagacgat
     1381 ccactacgat cactaaatca atttgtttcc caaattgttt gaattccgct ctttttcgtg
     1441 ccaaacgatt attgatgtct ccgttataac gctcaatgtc tgctaatgac caggaagtat
     1501 cataatactg attatattcc tcaataattt tttgcatttc ttcttgttgg acacttgaat
     1561 ctcggctgtt ttcttctaat gaataggtaa tagcgattcg tggaaaatca ggatcctcca
     1621 tcgttcttcc ttcacgaatg ggctgattag gaaactcttt tgttaaccag tcaggttctt
     1681 tcgtcatttc tttgatcgct cgataatact ttttggccat gtcaatggaa ctcgtggtta
     1741 agatcgcaga cttcgttgga cggccatttc gaaaatcaaa ttttgtatag gcattgtctg
     1801 gacggaacat tttatgaatg actttttgga tatgttcatc tgcttcaaaa acagtagggt
     1861 ccaaataact ttcttttttg acaggctcca tttgatcaat catacgattg atctgttccg
     1921 gtgaatactc tgcatatgtt tctacttctt ttaatttccg atagatttta ttctctaatg
     1981 aagtcggctc cacggtgttt tcatgctcta cttgaaatcc taaaaccgag ccatcttcta
     2041 atgcattctt gatggtatag gtatgcaaga cctctccata ctgatcatgg gtcgtacgtg
     2101 ccaattgccc tttggcttgt ttccgattct cagggaaaat cggtgtacct gtaaagccaa
     2161 accaagtgga tttcggaaac atttttttga tttcttccat attttcagca cttaacgctc
     2221 gatgacactc atctactaca aacacgatat gttgccccat taatttttgg aagcgattgg
     2281 ttcctttttt ctcttcttgt ttttttgcat atttcaacgc cgcatctagc ttttgacggg
     2341 ttgtaataat cacaacattt gcattggcat ctgccaacag agtttcactt aactctttgg
     2401 cacttcctgt tcccacgatc aagctattcg ctttggcgtt cccggaagaa atcccagtat
     2461 taaattccga agcaaatttc gtaaactctg tggtggtttg attatctaaa tctttgcggt
     2521 ccactaacat gatcgtccga tcgatccccg attttcgtgc taaaagctta gtagatacaa
     2581 aactagtcaa cgtttttcct gaaccagtcg catgccacac atatcctgat tgatgtttat
     2641 ttgcagcaat aaacaaggct tgaatcgcat gtacttgata aggatgtaac accatgagga
     2701 ttttattgtc ttgatcttca ctgacaatcg tataatccgc aatcaagcga tgagcatccg
     2761 ggatatttag cacttgtttg caaaactcat ataagttttc taccttttta ttatctttcg
     2821 ttcgccaact aaataaaaac ttaggatgca aatcctttgg taaggcattt gcaaaatacc
     2881 gagtggtttg ttcattggaa accacaaaca actgtaaggt cgaaaagata ttgttccgaa
     2941 acataccttc ttctgcatat tttttgattt ggttaaacgc ctgataaaaa ctatcttttg
     3001 cggttacttg tttcagctcg atttggacaa tcggtaatcc attgatcagt aaggtgacgt
     3061 caaaacgtcg atcccgtgct tctatgttac ttccacgttt tgcaatttga tggaccactt
     3121 cataggtaga gattcctcca ccaatgtctt gattagaata aagaatgagc gagacagaac
     3181 ccagttgcgg atcttcccgt tcaatcgtga tccgagccat tccattttca ccttttaacc
     3241 atttcgcagc atcaaagggg gttttcgttc gtagtaacaa ttctgttttg atcgtttcaa
     3301 attctttatc ggtcaaagga aaatcattca gttctgcttg attatttgag atgatttttt
     3361 ggcgtaaatt gacccaaaga tcttcttctg attttagatc gggacgatac gtccactgat
     3421 tatgcccttc ccccaataca cggatcaact gttcttcgac ttccgcttcc tcacggtgac
     3481 taattttcat ttatcttccc tccctaaaca aacatctttt gtaagaagcc ttttttcgtt
     3541 tccttcagca aatctaactt acgttgatga agagcgatcg tgtcgtcgag ttgtttgaag
     3601 aaagcgccga ttttttgttg ttcctcttta ttcctagtaa aaggaaactt gatattagcg
     3661 aaattctcat agaataagta ttctcgtacg cttccttcag tgttgcgctt gacctctttg
     3721 ataaatgttg gtgacttgat aaactgaagt atgaactcat tatctaagtc ctcgctcatt
     3781 ttcacaacta cataaagaga gctaacaatg accgtcatac caagattgtt aaatgcaatt
     3841 gatcctacgt ttactcttgc tggattatag gcgaattcat ttggttcaac gaatttataa
     3901 tttgtttttt ctaaatcatc caacctacta ccatcaaact gttctgtttg agaaattaaa
     3961 cctgctttat tacttaccga ataggcaggg aagtctaaat ctttcgtgtt cttttttcca
     4021 gtcttcttac taaactcttt gaacttacgc tgttcccaat cttccgtaaa tccaggaaac
     4081 cgaatttctg gcacttttgc tccgttttta gggaacattt tttgtaagaa tccttttttc
     4141 gtttccttta ataaatctag cttacgttgt tgaagagcga tagtgtcgtc cagttgtttg
     4201 aagaagttcc caattttctt ttgttcttct aatattggaa attgtactat ccattttttc
     4261 acttctgaca ttggtacgag atttgtaatt gctgaacctg gattagctcc tgtgagtttt
     4321 ctttgcataa aatctgcacg aataaattga ataaaatatt ctgaatctgt gatttcttta
     4381 tttattctaa gtctaatcaa agcctgattg aatacacccg gttttatttg ctttggtact
     4441 cttgaaattc tcccaattgt tccagcgcca ctcatgataa aatcgcctgg ttctaattta
     4501 aatttgatta attcattata ttttttttca gtaattttat atcttgtttc gtaattatca
     4561 taaatagcat tttgttgttc atagacaaca aaattgctat tagatacaaa gaattctttc
     4621 tttaaggcac tcccaaatgg tcccctcctt actccatctt ttttatcaag taagcattca
     4681 aacttacgct gttcccaagc ttccgtaaat cctgggaaac gaatttctgg ttgtatatca
     4741 ttactcatgt ttgaacacct ccagcgcacc ttgtaaccac gcttcatttt caggtgttgt
     4801 ttgcagggaa gagatagctt ctaaaagttc tttttctagc acttgctgtt cttgtcgtat
     4861 cttcttgatg tcatttccga catgaaccat atcaattggt tcttcttctt caaacgtatc
     4921 tacatagcgt ggaatattta agttatagtc attttctttg atttcatcaa agtttgccaa
     4981 atgagcgtat ttctcgacat cttttctttc agcataggtt tcgaggattt tttgaatgtt
     5041 ttcttccgat aatttattct gattttttcc tttgacaaat tctcgactag cgtcaatgaa
     5101 taatacgtct cgagtttggc ggttcttttt caatacaatc accgtcgttg gaatcgatgt
     5161 cccgaagaac aagttcgcag gcataccgat cacagcataa atactgccgt cttctagtaa
     5221 tttttgacga atgacccctt ctgcagcgcc acggaataaa actccgtgtg gcaagacgat
     5281 ggccatagtc cctgtttcct tcaaatgata gaaaccatga agaagaaacg caaaatctgc
     5341 tttggatttt ggcgctaatt ttccataacg attaaaccga gaatcgtcta agaaggtcgt
     5401 atctgctgac caattagccg aataaggtgg gttcattact actgcatcaa atgtgtaagg
     5461 ttcatctgtt ggccaatcct tattcaacgt atccccattg cggagattca tttcttcggc
     5521 atctacccca tgtaaaatca agttcatttt tgctagattg tatgtcgttg tatttaattc
     5581 ttgcccatga tatttaacgt tatctgggtg agttaagtag ttacgcacat ttaacatcaa
     5641 tgaacctgaa cccatggttg gatcaaaaac actaaagaat cgtcgttctt tttgatccaa
     5701 cgtcacgatt tgtgccatca tatcagaaac catatgaggc gtgtaaaatt ctccggcttt
     5761 cttccctgct tctgaagcaa attgactgat cagaaattca taagcatccc cgatcacgtc
     5821 cccatcgtga cctaaaactt ccacgtcatt caatttctta atgacttcgg taatcgtcac
     5881 atttcgttgt tgctcatctg ttcctagttt ttttgattgg agatccacat catcaaataa
     5941 cccattaaat tgattgtagg tagatgccaa ttgcacaaag gctttgttta gatcattcaa
     6001 ttggaacgtt gcttgttttg cttgatccgc taagacgttg aaaagatact ctggcgaaat
     6061 cgcatacccc aaaatatcaa cgattgttgc aatcagatct tcttttgatt cttcatctga
     6121 aagtaattcg cgatacaaca tcgtttgttt gcttaccgta tcatattcct ctaaggattc
     6181 atctgctaaa agaaccactt gttccaacaa acggtcggat aaatatttat agaaaatcag
     6241 tcccaataaa taatttttat attctgacgc atccattttg ctacggagat tatctgccgc
     6301 actaaataat ttttgattta attctgcacc caaaaagata cactcctatc tcttatttaa
     6361 agttgctata taccctctta ttttagcata ctccctttta gaaaaagcaa aaaaaaaaag
     6421 aaagaaactt attcttgcta ataaactacg cgaaaggagt ctaacgttgt ttgatataac
     6481 ttagactgat cgttaactta ttttttatat ttctacatag ttatctttag aaccatttca
     6541 gatacatatg aaacaattac atatgatttt ttcataaaaa aacagtactg tctagtagta
     6601 taaaatatac aaatttctta aaaatttttt cactaaaatc actacctgaa acagtctaaa
     6661 acacttatat atcaacgttt aatgaaacta tctccgtttc atttcagata caaataaata
     6721 tagtcgggct ccaaatccga aaaaaaatac ccccagtagc ttatcctaag ctactggaga
     6781 tgtataagta cggtcatata acaacggggt agggtaaata aatgaaaaag atggaagaat
     6841 cattattcaa taactttttt aaatacttcg ttgtaatcag ttacctgtcc gttcttaaat
     6901 accacagaat aatctttttt cccattttca agtaaccgaa gtggaatatc tttaccaact
     6961 ttctctgtca ctgcattact gatacctgtt agattagcta ataaatctga taaagattga
     7021 gcatctgtag aaatctctgt atcagtattt atcgtgattc ctttatcttc atttacaagt
     7081 ttccaagtag gtggtatagc tgtgtatgtt tctacaaatg aattaagttt ttcattaagt
     7141 gacttctctt ttatatttcc atctaaaaac tctgtatata tggcttgagc tatactaaaa
     7201 caaagctggc cttgttctaa taattttaca tcattagtta gtaaagctgt gttcacgtta
     7261 tcaatcgctt caagaagagt atctttggtg tccctaacag ctttaggtgt gccttctgga
     7321 atttccctac ttgatacctc gtccatttta tcttccataa accccttaaa atcatatctt
     7381 tctcccaaat ccatagaagt ttttgagaaa gctttcattt ctgagtctat ttcttcaata
     7441 atatttaaca tgtctgatac atattgttta ggctgattcg tttttaatct atatgtggta
     7501 aatatctcaa ttcctccaat tatcaataaa attgccaaac aaatccataa taattttttc
     7561 tttttcattt ttcctcctat acactgctgt tataacagtg actaatgtaa agattttccc
     7621 agcaatcatc agactattat ttacagggaa tcaaaaaaac tccttagtaa gcttacacct
     7681 ttaggagttc ctgtttgaac ggtatgcata atacatttaa atcaatttaa cattaacacc
     7741 tcagtatatg gtatctctac atatacacgg tctttttttg gatagtactc gacatttata
     7801 ttgatagcac ttccattgtc taacgtagct ttagcccggt acgtgttatc accattatca
     7861 tgagtgatcg tcacatcatc acatgtccga tttaagttat actgttcttg taaaatctga
     7921 gttaccaatg gtacagatga cttctctagt ccagatgaac ccgtcattct tgaaaaaatt
     7981 atacctataa tagccaatac aacgatacct ataatgacaa acttcttatt catatttact
     8041 ttattcaact ttttatctcc tatctccaaa atgattttct ctcatcagta actcattgac
     8101 atccccaata gtacaccaaa gatggctcca gctacccctg ctatcatcaa aatcgtaccc
     8161 atttgttcat ctttctctcg atataagtaa cccataataa cagcaacagc accaaatacc
     8221 acaggaataa atgctaatga tactaccata gaaatccaac caataatcaa atacagcttc
     8281 cctgtctctt gtccatgtac gatttgttcc ttcatttcat taattgtctg ctcagtactt
     8341 ttctttactg tgtctgttcc ggttgtctca tctgtttctt gtaatgctcc gcaaaactgg
     8401 caatacttac tttcatttgt tatttcttta ccacattttc tacaatacat gtcttctcct
     8461 cctaaaatca ttcaattgct tattagctaa ctgtttactt tattaaatac atcagaaaac
     8521 agctcattta ttggtacact taatatctca gaaagcattt tcatttgata gggtttagga
     8581 atcgttctcc ctgtttgcca cgaacttaca cattgctgtg taacattcat ttttttggct
     8641 aactcagttt gggaaagtcc tttttctttt ctgtatcggc tgagattact tacttttttc
     8701 atgattctcc cctcctgttt tattatatac aacaatatga tgtatttcaa ctcataaaat
     8761 atataaatac ttcaaatgtc attacaaata caacaacttg ttgtactcta ctgagaaggg
     8821 gggtgtatta atgaaacaga agcaatatgc aaaggtcatt agagaaaagc gaaaagcact
     8881 cgggtggaca caaaaagaat tagctaaaaa aatcttctct acacaacagg cagtagcaag
     8941 atgggaaaat tctgtaacag aacctaacct cgatagctta acagctcttt ctagagccct
     9001 aggaacccct gtaagccatt ttctagacaa tgttgacata gattatgagg aagagttttt
     9061 ggctctctat cgctctctta gtactgaaga tgcaattcga acaattgact atatgaagct
     9121 attaaaaagg caagaaaatg aacgcaatca gctattgaaa gattgaaaaa taaataaccc
     9181 accgaacttt aacggtgggt gtattgagat gaaataagat taagagctat ggataacctg
     9241 tctagtttaa tataattggg tctcttctat tccttactct ccgtccatca tctcttgtta
     9301 cacttgtaaa ttgataaata ggaactaatt caactcctga taatatcaat tcttttatta
     9361 cttcatctat ccctgcccct ttcgctagat atactaaaaa actactgctt gctttcacat
     9421 tgtatagttt cttaatatcc ttagaagctc gttcaagagt gaatccttgt ttaacaaagt
     9481 agcctctgtt ttctattaaa gcatttttca cgctgttatc cctgacattt cgtaggcgtt
     9541 cacaaacagt gttaataagt tctttttctg tcattagata tgtcccttgc cacgataagc
     9601 cctgtaaaac ttcatgtgct tcatctaaaa tcaattgttt ttccttgcta agttctgatc
     9661 gggaattttg aggaaatact cttttagcct ctttttcgtt aaaacatatc ttaacccatt
     9721 cataagaaaa atattgatat gtaaaatgaa atttttccag aagtggaagg atatcttcat
     9781 tcaatttttg aatcacagaa gttgcattaa aaacgtctaa tcgatagaca ttgcttctct
     9841 tatgtttaaa gtttcctaac gctttctctt cctttaacct atttaggtat tcgagcatct
     9901 tcaaaggaac atcttcatca tctaattttt ttatcaatcc tataaaagtc ataagtgata
     9961 gtaacttatt acatttatct atgtcataag ttttaccata gtaagaactc atttttttag
    10021 ctaattgttc ccccgacaaa atacaagtca cactttcctt actatcatca aaaaaattaa
    10081 catcagaaag caagacatca tagacacgtc gcatttcctc tagataattc cttattgtcc
    10141 tgaaaagttt gtctttcatt gattcaagag tgtcaaaaag tacttttgat tcctctttca
    10201 attttcgaaa atgtgtagga tacaagtcta ggttctctaa tagccaatta aatgtttcta
    10261 cccgactgtc tttctttaaa attagcatca caactccaat aaaattttca ctgatatcac
    10321 aattactgga atgacaataa aaaatggaat gcccagtttt actggtaaaa atactagcag
    10381 agggattatg atcgttgtga aacagacatc ttaagttttt acttgactta agtaatttat
    10441 tcatatcaat ggttaacaat ctttcataaa tctcattaaa agacatgtcg gaggaatcaa
    10501 acaggcagtc tttaaaccac tgtttcattt cttctttgtc gtcatttttt atcaacttaa
    10561 caaaatcggt ttcattgact agttcatact ctctccctga atcactttga caatttttag
    10621 aacatgtcat ggtaaaatca atcggaagct cagtaatatc taacatgttg tcttcattaa
    10681 ttattatcac tgcatctgac ttatatcctc caaaaaacat tctattcgaa gcatttacat
    10741 tgtcatcaca cccggggatg ttctttttca agtattcgta aacagcaacc attttaccat
    10801 agtcagtaat tgcttctttt aatagaaaga caatcttgta tttttctaga ttttcagagt
    10861 accgaatact tttttgtatc gcaaacgcag aattatgaac taaattgata gataaaacct
    10921 tttctatgct taagtatcta ctatcatctt tactcaaatc tactaccttc cccttctcat
    10981 ctttaacagt gttatcgaca tccagaataa ttactctggt ttccacaaaa ctctccttgt
    11041 cattgccttt attctttgtc ttgcttggaa taaatgattg tgatgaattt agtatctcta
    11101 caaactcatc tcttgtaact tctctatagt tatctaaaca cctatctctt acatcagttt
    11161 tttctttttt tgtaactggc ttctctaata atttttcttt atctacataa attggaatat
    11221 taatcattga tgtttccatt tgtgtttcct ccttataaaa ttctgttaaa gactaaaaca
    11281 tacagataat aaaaacaaac tctttattac tatatgtttt agtcttattt gtttactcaa
    11341 cctttatacc gttccatgaa actatcaata tcagattttt taaatctaat gaccgtatca
    11401 atttttataa caggtaaccc gtattgagaa acatattttc taatcgttcc tactgataca
    11461 cctagatatt cacttgcctg ctgatagtcc atatactcta gagtgactcc agaaataatg
    11521 tcttctagca taggctttag cactgtaatt atctctttca cttgttgttc aacttgtata
    11581 gaaacaatgc tatcaatgat ttgtagagac atatttcccc ctcctttcgt gattgcttga
    11641 acaaattttc taagaattat cttcgtattt tttgctcaaa ataaaagtat tttgttatta
    11701 accgattaat caaacaaaaa atgattacca gttcatcgac atacacataa tacctcgata
    11761 aaaaaggggt gtcaatagtt ttgtaaaatt tatttacaac accccttttt tgaggtatac
    11821 taattttagg aggtgtggtc aatatgataa aacgtccacc aataaattat ttagaacgaa
    11881 aaaagatact cggaacaaaa ataaaagcta taagaaagag taaaaaatta acacaaccag
    11941 catttggtct tatgattaac aacggtcaat taattgacaa aaagactatc tacgaatggg
    12001 agaagggaac ctatttacca ataccagagc gattatcccg aattgctgat ttgggaaata
    12061 tgagtataga ggagttagtt tgtggcaatg tagaagaata tattttggga attattcttt
    12121 atagagatag cattgtacta gatggtataa catttccaga taaaaacctg ttccagcatc
    12181 ttcgtcaaca gttcccacca gtacactcta atttagatac atggctggat agatattcca
    12241 aattagaacc agagatgcaa gaatttattg ctaacaagac ttgtaataaa gttaaaaatg
    12301 aaaaaattag tcttttcaat atcttaaaaa tagaagaatt attcatcaat gctattgtag
    12361 aagaatttga caataacatt cttttcctca ctagcagtat agaagaacta ctagaaagaa
    12421 tggtagatga atggctacct attcaattga aagacatgtc ttatcccgaa gaagctgtga
    12481 gagaaattac tgataatata aacaagctag aacaaacgat ttcatcaatt gggaaaaaat
    12541 acactaagaa aaaaatgaag ggaggtgata ccatctagtg gtaagtactc cttgtaaact
    12601 aagaggagat gatttcatgg caaaaatatc aaagtacaaa aagaaaaacg gtcaagtggc
    12661 gtggatgtat aaaggacaca ttgccaccga tccacgaact ggtgaaaaaa taaatactac
    12721 gagaagaggt tttagtagca aacaagaagc tcaaaaagat tatgacgagt acagacatca
    12781 aatattatac ggtgttaaaa agaaagcacc tgatatgaca tttgaagacc tgtataatga
    12841 atggataact catcagcgta ccagtgttaa agcttcaaca atcgcaatct cagtaagata
    12901 cgcaaataat caaatacttc cagcatttgg taagcttaaa ctatcgaata tctctgtccc
    12961 atattgtcaa aaagtggtag atgaatggca cagtaaatat gaaagttacg attatatgag
    13021 aaaacagact gcacaaattt tacgctacgg ggttgcaatg caatatattg acaataatcc
    13081 tatggaaaaa acacttcttc cacgaaaaaa agagtatgag aaaaatcgta aattttactc
    13141 aaaagaggag cttaataatt tactggatgc ttttaaagat tttggtaaca tgaaacaata
    13201 cgccttcttc agattattgg catacacagg tatgagaaag agtgaagtac tcgctttaca
    13261 gtggaaagac atagacacgt ttaataaaga actacatgtc aataaaactc ttgctgtaga
    13321 tgaatttggt aaagttataa ttcaatctcc gaaaacaaga gcttctagac gagttatctc
    13381 tttagatact gagactcttt ctatcctcaa caactggaaa ttacagcaaa aagaagagta
    13441 tttaaaactt ggatacaata ctagctctaa agagcaacat gtttttacaa cagttaaaaa
    13501 cactctatac attccaaaca ctgttaatga ttggttaaga tatattttga aaaaatataa
    13561 cttgcctcgt attacacctc atgggtttag acatacacat gctagtttat tattagaagc
    13621 aggagaatct gtcaaggtag ttcaacaacg attagggcat gaaaatagta aagtgacatt
    13681 agacatctat gcacatatta caaataatgc acctaagaaa acaggtcaag actttgctga
    13741 tatgatggct catcagtaaa agagctggtc aaaatctggt catttctagt atacaggcaa
    13801 aacaaaaaag ctagaaccct tatatatcaa gggttctagc tcctataaca ggtttaccca
    13861 acactgcctt ccaatgactc ttttgggaat cagaggaaag aatcaggctt tatagagata
    13921 tttcatctaa caaattactt tagactgaca gtatatatca gcaggtactg tcatcgggaa
    13981 cgtagccaat atcgtatcca aaacagcagg tagagaagct atttgaggat tcaagttatt
    14041 ctatttcaac catgaagtta agttcattaa aaataaagat agttggtatt tcaataattt
    14101 ttgtatagag ttaatcactt tattgatgct aattcatctt gattggtatt ataatcgtat
    14161 tcaagttgcg tggcgtgctt ttgctagaaa atatataact gttctttata aagaaatata
    14221 aggattaaac attttttatc atagagtttt acttatctat agaattatgt ctgttcctaa
    14281 ttcagtttga tgaattaact gataaaaaaa tatctactat ttaaagatac agggataagt
    14341 aagtaaaaaa ataacatgtc attagatagt agacagttcc aactctttat ttaccttttt
    14401 tgtatgctgc actgtcaatc caagttccta aattgttgta tccatcatca cttatataat
    14461 catattggcc atcaataatt tcgtcaaacc acaaatattc tccatctggt ttttgcccaa
    14521 ttattttaat tactgattga ctagtagttg tcatctggtt aaaatctttt attttaccta
    14581 tattaacggc caaaatagca tttcctcgct tataactctc ctggatttca tatttaacat
    14641 attctcgatc aagagtttct ttacctatca gcaccacagt cactgatgtt ccaattaatt
    14701 gattatctat ccagtttttt atagcaatat ctccattacg tttaactttc tcaaattcag
    14761 ccttatctac aaaaccagct tcctttgttc ctcgcaaagt ccagctgttt ctaactacca
    14821 ttgctcttgt tacatctgca taatggaaac taaaaaaaac ccttctcacc atattaatcc
    14881 tcctgaatac tatttattaa actgacaatt tgttcaactt ttttatctac ttctccttca
    14941 gtatctaatt tttctagcgc ttgaaattta ctttttaagg aatcatcaaa ataatcactg
    15001 aaattctctt gaatcttatc aaacaaaacc ttacttgcac ctttggtaaa tccaatcgga
    15061 actactatat tttcattttg aacagataat tcaaattctt caatcattcc atctgctgta
    15121 actatttcac ctgattcaag tttgtttcca aaaacaaaaa tactaatccc agactctgca
    15181 ataatgtttt ctcgatatct tctccactca tgaggcatat caaggggaag tggtcttaaa
    15241 gaaagtacgt cttgtatgtt attttttctt aaatttcttc tctgctttag aactcctgaa
    15301 acaatactat taccaattcc aagtccgtaa cctgatacta ttctataacc ggattcagaa
    15361 agcctatatg ctaatttctc gacaaatttt aatcctttcg attcagatgt aaatggagta
    15421 taatcaacag cacttcccga aataaaaata gtttttctca aatacttttt tctaatactt
    15481 ttcaaaatat cagtaatctc agaataatca tctacttcaa caacgtttat cgaaaatctg
    15541 gacaaatcat tgatttgtag tcttcttttt gtcagttcat aattatatct ctcttgatca
    15601 aaaaaatcct cagaatcgac aaaattttcc tttaatattt ttctcattat acaataatga
    15661 ggtctttggt tttctctcaa aacccatctt atatctgata aaattctttc taaattaggg
    15721 tctgtaaaac taaatcctaa aaagagaaaa gtttttgtta acaaatctcc ttttaaagtt
    15781 tctttaaata acgcgtacga atcatcatca tatttttcat aatcatctct tgttattact
    15841 gtcgaactga catcatctat atcaccatgc attttataaa caattgtatc cacatttgga
    15901 ttagttgttg ctaactgagt ataatttttc ttaacctgag ggtttttata agcttctcgg
    15961 attgattttt ctattagatg atcataatta gttgtccaat aagtttcaat aggtaaactt
    16021 gctagtattc gatgattttc agatatttct aattcttcac caaaattatc cataagtatc
    16081 tgattcaaaa ttcctcttga tccaccattt tcatttttat aatattgagc cagagtaact
    16141 aagtctgttt cttttttaga atccaacccg atttctgttg caggatcatt aagtagttcc
    16201 ggccaactta catatccttg tgacatcgat aatcctgcac ctgcaaatat tgctgcattt
    16261 cctttttcaa tagcttcaga gaacctcttt attaaaactt tcttatccat tctacccggt
    16321 tcctttcgat tcagtaaatt gattagagat tagtgaaaat ggtgttttta tataaaatat
    16381 ctaatgtatt acttccttta gtgcattcca cagcatctaa agcccatttt ttcaaatcag
    16441 cttttcgtaa ataagttaat tcacttaaat ttaaagaata ttttatttgc agattttctt
    16501 gcgaagaata gtgctttata atgcttctaa catcctcggt gctcatttga ggattacttt
    16561 ccaatcgtgg attctttttt tccactattg aagctgtcgt cagttcgcta tatagccatg
    16621 gcgaatacgt aaatttctgt tcattagtga tttcattgct caaactaatt gaattaggtg
    16681 tattaagaaa aaacagacat tctgtactat caatcatatt atttaaagct gtagaaagca
    16741 tcatatgaac atgactagtt gttacatttc ttttatcata atcatatgtt tttgtttgtt
    16801 ccttatagca atatttttgg tctatttttt ttaataaatc agaactgtat ccccaaaccg
    16861 aagaatcaat aaatgcagta agaccaaaat tttcaaatag ccatgatgca aaacgtatag
    16921 ctaatttttc atcagcatga gagtgagata taaaaatatc tgcttttata gttggaaacc
    16981 aatctttaat aatttcgtct gcatctagaa ttttgctgtc cttagaataa taatcaacta
    17041 aatcattttc actttcaaat agattttcta ttctcttgtt aatagatttt tggttgtgat
    17101 ctatttgatt tactacatgt gtatatgatt gcgagcttga ggagaatgca tttaagtcag
    17161 catttgtaaa atccaattca tatgccttat acaaggatat ccctccattc ttgtattttg
    17221 atccacaatt tattaggcaa cataacagta taccctatcg ttacatatcc cattcattat
    17281 tataccagta tttcttcgtg acacataagg gtgtaacata ataatttcaa acgaaatgac
    17341 tacaagtagt cattgaagca aatcttcata agacacctgt agaatatctt tgatggcttt
    17401 caattgatcc aacttaatgt gttggcggcc accttcaatc ttaaccaacg tttccctagt
    17461 tatagcaatt ttacgaagct gtaggtctct aatcagctcg gtttgaccaa ttcctttttc
    17521 taatcggact cgtctaatat tgctaccgat tacattttct ttatcaataa ttttttcctc
    17581 tttcaagaat agcacacctt ttggactaaa attagtcctt tttcttatta atcttaaaga
    17641 ttcggtatgc tacaatggga ctagtattag tccatattca attttaagga ggaatttttt
    17701 tgaagaaatt tgcaatcatt ctaacttgcg ctttactcat cggaactata agcgggtgta
    17761 ccacttctga tacaagttct acaagtacgg aaactagtac aacaacctca gaaaagaatt
    17821 acccaataca tttttttccg gttccagatg gatttgaagc ttattatcat gataaaaaac
    17881 tagatgttcc tttatcagaa actgaatcta ctgaaaagat tttgccaatt gaaattgaaa
    17941 gaaatcaaga cgatataaaa ctaaaagctg atcttccctt actgaacagc acacaaattg
    18001 atagacatga aaatattaaa gttaatattg aaaaaggagc gttagcacaa ctttatactg
    18061 aaaaagttga acaatttttt gaggctggtg ccaataaaga cttttcaaaa ataagcgatt
    18121 attcggataa cttttttaat gagacccaag atgaaatcac gaactcaact tttatggctg
    18181 aagatatgta caatatgaag ttgaaaaaaa taaagtcgta tgattatcca ttaaatctaa
    18241 cattaaataa agggatactc tcttttgtat tagcaggtta cgttgactat gaatatacag
    18301 cagattattt ggagacgccc cttcaacacg ataataacga tattaatttt agatttatct
    18361 acaattcaaa aagtaaaaca tggttagttg atcaattgag cactataaat acgggatttc
    18421 atgaattacc taagcctgac aatgaaactg ctttggtcat taaaacattc taaaaaagaa
    18481 acgttgaagg aataattatt ctttcaacgt ttcttttatt gtttttcagt ttacggtctt
    18541 acctgagcga ataatctgat ttctaaatga ttggaaaact tcttgtggtt gataataatc
    18601 tgtatctatt tcaaatgttt ctgagaataa ttcattgaat ttttctactg aaatgatgat
    18661 tttaccattc tcctctcgac tgttttctat tatttctgcc tctaattcaa ataaatctag
    18721 tgacgaacta aaatcataaa tcctgtcata ttgaacaaaa aaatcaagaa aaagttgctc
    18781 aaactcttcc ttatttatca ctagtgtttc aagcggaaca actttatttt catcagccat
    18841 tcttccaatt tcctcacttt cattttcaat tgtccattgt tgcggacgta tcagattttc
    18901 tctatcattt ttcagtgtat agatcacttg taatacacaa caatgctttg taaacttatt
    18961 tttttagtta gtcgaatctt tttaaatgtg cgtttcatac catgcgcttg atatattttc
    19021 tcctattttt taaatcgttt atcacaactt ttatcgtttt ttcttctgaa aaaaatcttg
    19081 tttacatcgt tacatagttt ccaaaaagat acattactag tagaaacaac aagattaatt
    19141 ttacttccca ctgaaatggg aagtatattt taaatatttt ttctccttca acttacacta
    19201 taaagtatct taatcacacg atatgctagc cactaaattc atcatatttg attccttttt
    19261 taagataatg tagcatcttt ttatccaata acgcaattgt ttttaaggat aaatttctta
    19321 agatggtaca tttcaaaatg gcatttgaca tatgaaacaa aaaagaaaga aaagagcata
    19381 agggaaaact gatagcacct tcctacagga aggtggctaa gttggctgtg ccaactggtt
    19441 ttctttcaaa atcatttcat aatttttgat atcataaaaa aatccatttt ggacctattt
    19501 tcggtcataa tttagtacct acttttggtc atagtttcgt ccgtagtttt ggtcatagtg
    19561 actaaggctt ttctgttcac aaaattggtt taaatttgct ttctttaaca cttcatttat
    19621 atcatagcaa ttataatgtt tacacaacat cattcctaat tttaacggtt gttcctcttt
    19681 atatttttta agttctacat ttttttgcgt ttaattactt ttctcttttc tttatattcc
    19741 gctccaactt tctctttaat agtgataata gaattacata tttttgttaa acttccttaa
    19801 aaaaattgga tctcgattac tcaaatttgc ttgcaggctt ttctaattgc tttgtttttt
    19861 cttcctaatt atttgatttt atttccattt tcttctagtg aaaatttatt atttttaata
    19921 atcgtacgcg tagaactgat ttcatttttt gtatctgttt gattatctaa cgtttgaaat
    19981 tcaaagtttt gattggcttt atcaatatct ttttatacta ctttatttgc aagatatatt
    20041 ttcttgctta atttggtcat agtttcattc gtcacttcgg tgatattttt ttaatttgca
    20101 aataaaaaga gttaaaccga tatacattag gttcaactct tttgcaggtt tcatgggtgt
    20161 actatcactt ttaatctgat agatgtaaac ctctctttat ctttttgtta cttcaatttt
    20221 caacaatagc tcctttatag gttgtcaata ttgctctacg tattctggct ttttaaccca
    20281 ttatttctct aaagtggaag agaataatcg cttaatcccc actaattcct tgtcgtaaat
    20341 tcatgtcaaa tacaattttt aaaagccatt taattcatgg attatctctt cacttttttc
    20401 tagataacaa tatttcaagt tgttttttat ctcatgtttc atctttaatt cttcttgaat
    20461 catcagttca atataattag tgaccgtgat tttttcattt gttgcgttct ttactgattc
    20521 acataattcg tcaagttcta agaaatcatg cgtactaatc tttagaatta atttcatgac
    20581 aattccccct tttatttttt taatagatta tcgaaaagcg gattaggatc attaagtgta
    20641 tctctgaata actcgttgtc ttcataggta aatcttcgtc ttggttttga tcccttatca
    20701 ttttcacgga tttcactatc aataatcact gtttcatgat ttgaatcaaa ttttttcgct
    20761 gactttctgt cttcaacgat attgattttt tgtgtttttt ctttcatttc tccactatct
    20821 ttttgaacgt tctcccgaac atctactgtt ttgaatcctt gactacttgt agaactttgt
    20881 acattctctc tt
//
LOCUS       DBNICI010000035        20709 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.035, whole genome shotgun sequence.
ACCESSION   DBNICI010000035 DBNICI010000000
VERSION     DBNICI010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.035"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..325
                     /gene="sgrA"
                     /locus_tag="AC1ZCC_001046"
     CDS             <1..325
                     /gene="sgrA"
                     /locus_tag="AC1ZCC_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HIQ1629313.1"
                     /translation="SSESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSV
                     LKQPDFVSRGIDHQEKAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSL
                     FHRVK"
     gene            623..979
                     /locus_tag="AC1ZCC_001047"
     CDS             623..979
                     /locus_tag="AC1ZCC_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629314.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            1111..2139
                     /locus_tag="AC1ZCC_001048"
     CDS             1111..2139
                     /locus_tag="AC1ZCC_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629315.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(2165..2605)
                     /locus_tag="AC1ZCC_001049"
     CDS             complement(2165..2605)
                     /locus_tag="AC1ZCC_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HIQ1629316.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            2765..3271
                     /locus_tag="AC1ZCC_001050"
     CDS             2765..3271
                     /locus_tag="AC1ZCC_001050"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIQ1629317.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            3439..5352
                     /locus_tag="AC1ZCC_001051"
     CDS             3439..5352
                     /locus_tag="AC1ZCC_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIQ1629318.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            5357..6358
                     /locus_tag="AC1ZCC_001052"
     CDS             5357..6358
                     /locus_tag="AC1ZCC_001052"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIQ1629319.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(6560..6766)
                     /locus_tag="AC1ZCC_001053"
     CDS             complement(6560..6766)
                     /locus_tag="AC1ZCC_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HIQ1629320.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(7412..8260)
                     /locus_tag="AC1ZCC_001054"
     CDS             complement(7412..8260)
                     /locus_tag="AC1ZCC_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIQ1629321.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            8424..9326
                     /gene="gnd"
                     /locus_tag="AC1ZCC_001055"
     CDS             8424..9326
                     /gene="gnd"
                     /locus_tag="AC1ZCC_001055"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HIQ1629322.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            9328..10329
                     /locus_tag="AC1ZCC_001056"
     CDS             9328..10329
                     /locus_tag="AC1ZCC_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HIQ1629323.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            10326..10610
                     /locus_tag="AC1ZCC_001057"
     CDS             10326..10610
                     /locus_tag="AC1ZCC_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HIQ1629324.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            10643..11440
                     /locus_tag="AC1ZCC_001058"
     CDS             10643..11440
                     /locus_tag="AC1ZCC_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HIQ1629325.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            11454..12287
                     /locus_tag="AC1ZCC_001059"
     CDS             11454..12287
                     /locus_tag="AC1ZCC_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HIQ1629326.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIVGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            12305..13162
                     /locus_tag="AC1ZCC_001060"
     CDS             12305..13162
                     /locus_tag="AC1ZCC_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HIQ1629327.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            13184..13693
                     /locus_tag="AC1ZCC_001061"
     CDS             13184..13693
                     /locus_tag="AC1ZCC_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HIQ1629328.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            13730..14131
                     /locus_tag="AC1ZCC_001062"
     CDS             13730..14131
                     /locus_tag="AC1ZCC_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629329.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            14243..14596
                     /locus_tag="AC1ZCC_001063"
     CDS             14243..14596
                     /locus_tag="AC1ZCC_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629330.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            14630..15073
                     /locus_tag="AC1ZCC_001064"
     CDS             14630..15073
                     /locus_tag="AC1ZCC_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIQ1629331.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            15090..16175
                     /gene="uxuA"
                     /locus_tag="AC1ZCC_001065"
     CDS             15090..16175
                     /gene="uxuA"
                     /locus_tag="AC1ZCC_001065"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HIQ1629332.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            16168..16881
                     /locus_tag="AC1ZCC_001066"
     CDS             16168..16881
                     /locus_tag="AC1ZCC_001066"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIQ1629333.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(17088..17237)
                     /locus_tag="AC1ZCC_001067"
     CDS             complement(17088..17237)
                     /locus_tag="AC1ZCC_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629334.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            17633..17833
                     /locus_tag="AC1ZCC_001068"
     CDS             17633..17833
                     /locus_tag="AC1ZCC_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIQ1629335.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            18401..18964
                     /locus_tag="AC1ZCC_001069"
     CDS             18401..18964
                     /locus_tag="AC1ZCC_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629336.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(19025..19390)
                     /locus_tag="AC1ZCC_001070"
     CDS             complement(19025..19390)
                     /locus_tag="AC1ZCC_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HIQ1629337.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(19466..19711)
                     /locus_tag="AC1ZCC_001071"
     CDS             complement(19466..19711)
                     /locus_tag="AC1ZCC_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1629338.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(19781..20035)
                     /locus_tag="AC1ZCC_001072"
     CDS             complement(19781..20035)
                     /locus_tag="AC1ZCC_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629339.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(20199..20522)
                     /locus_tag="AC1ZCC_001073"
     CDS             complement(20199..20522)
                     /locus_tag="AC1ZCC_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629340.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT         7306 a         3203 c         4226 g         5974 t
ORIGIN      
        1 gagtagtgag agtagcacag gaagcacgag cagcgaaagc agcacaggaa aagtaaataa
       61 tgaacttgat ttggtaccta tcataccaac taaggaacaa aagagaaatg cagggagcaa
      121 cctaaaaagt gtgctaaaac agcctgattt tgtttctcgt ggaattgatc atcaagaaaa
      181 agcgaaagaa acagataata gaaaaagcga tttaccagaa actggtagta gaaccttgaa
      241 caagctaatt acatggatgg gtgtgttact cattttgatt gtaggagcaa gctattttcg
      301 ttctctcttc catcgagtga aataaagaat aaaagcaaca gatctaaaat tgaataaaac
      361 gatagatttt tgagtgaata tttcattcga gagtgctgta ttgcctggcg aagttgtagc
      421 tttgccagat ctatttttgt ttactaacat atttgagaaa caggaaataa caggaacaca
      481 aatggaaaat aagagcaaga ataattgaca atgcacactg cgcatggtag tatctctgaa
      541 aaatcagtaa cgaatctatt tgtacgttat tcatgtagct gtcggaataa gtctaaataa
      601 tagataaaaa ggagtatgta ctttgtttga agaatttatt gatatcaatg aaaggcaagt
      661 atatcaattt ttgaactatt gttatgaaag agacgagaaa ttatatgtgg tgaaggatat
      721 cgctttagat ctaaactaca ccctggctaa gatgaattcg gtgattcaac aagcagaatc
      781 gttttgcgaa aggtatccag aatataaatt atcttttctt tcagaaaaca agatgataaa
      841 agtcgagttt tcttctcagt ttttactgag taaagtttat tctatacttt tagagggaac
      901 gattggttat attttactgg attcacttta caaaggtact tatcaatcct tagaaaatct
      961 tagtcaaaaa attatataag tttgcgtacg attcaaagaa aattaagaga acttaatact
     1021 attttgaaaa actatcattt atcttgcagc ttgaaaaaag gtaatccgat agaaggagaa
     1081 gaataccgta tccgatactt ttttcacttg atgtattggc agtcattcga cgaaaatcat
     1141 tctgatatat tatcaagtat aaaaaataaa cgtcaactat tagaaaaatt tgcgaaacat
     1201 gcgccatata cgcgtagtat agatagaaag aaatggatca gcctactaag aatttcgttg
     1261 caacgaatga aacaaggcca ctcgattacg tatgtcccag aagagatgaa caaatttgtg
     1321 catccattga taacaaaaga agagtttacg cattacatac tagagccttt ttttgaagca
     1381 aacttctggt cgatgaatca catcactcca acagagcaag cttaccttta ttttatgttt
     1441 ggtgtaatga acacctattt ggaagaagat ttgttggata ctccagataa agtacaagaa
     1501 agttggggct ggattgaatc gtctattgta gaaaattttg aaaaaaactt caagatttgt
     1561 tttacaactt cagaaaaaaa ttatctatat gtcaatttgg caatgatcca tttgtattca
     1621 agggtttttg ggacaaaaaa gaaggtggat gcttttggaa aatcagcaga agacagagat
     1681 tttcagcata tatttccagt gatgtatcca atcatgcaac agttttttag tggggtagct
     1741 cgcagaattc cagagcttaa tacttattat gaaaagaaca gacggttgat ttttcaatac
     1801 tgtatgttag tcagggatat ttttataaaa cacgaacaac ctgtcatatt ctatattcaa
     1861 tcaaagtatg gtaaaacaca ggaattatgg gcaaagaaaa ggatattatc tatgactgaa
     1921 cgcccaataa aatatagtgc atccgctgat cagttacctg atattgtcat tagcgattat
     1981 cctatcgaca aaaaggcata tgatcaaacg aatacacaga tattttactg gaatggacaa
     2041 cctacaaaaa atgattgggc gcgtctgaaa gctgaaatca taaaaatcag gaatgagaaa
     2101 aatagatcca tattggaaaa attggatttc gttaattaag aaaagcagat tgtttatgcc
     2161 aaaattaaga cattgcaaaa agattatttt ctttaaaatc gacaagatga ccagaaagca
     2221 agagttcgtt gagctcttgc tttcttttag ttttagaaag gaacatcctg attttatctt
     2281 cgctaaatcc agtcaaaagt tttgcagatt ggtcgtagat aatcggtttt ttcaataaag
     2341 aaggggatgt acgaatctca atatatagtt cactcaaact gagttcatct attctgtgaa
     2401 caagattttt ataactaata gaatgagtag agagcatttc ggtcacgccg ttgtttgtca
     2461 attttagtaa gtggataaat tcatttttgg ttagtggttg acgaattaag ttctgttcga
     2521 taaacggtat atcatggacg ataagccaac gttttgcgcg tcttgaagac gagcagccag
     2581 aacctgtata caatttaatc atcatacatt cctccaatag ctaagtattt atgaaataat
     2641 aacatgaaga aatagtcgaa aagtgccatt tagaggtgta aaataagaaa atgttgtcac
     2701 gtataatgga actagctaga taagaaatac tattaaatca agaaaaaaga aagaaggcac
     2761 aagtatgtca aacacagccg acgcaatagt agtgatcgat ttacaaaaag gggtatgttt
     2821 tgattcttcg ccgatacacg aattagaaac agtgacagca ttagtgaata aaagaatcaa
     2881 aacatacgaa acgattggga aaccagttat tttcgttcaa cacgaagatg ctcaattagt
     2941 gccacaaaca aaaccatggg agctattgtc ggaattatct attcctgagc acagttattt
     3001 tgtgagaaaa acccatgcga attcatttta taagactaat ttgagtgttt tattaactga
     3061 attggactgt cattcgatcg aattatgtgg ggcacaagca gagtattgta tagatacgac
     3121 tgtgaagttt gctcatggat taggatacca gcttatgatg tgtagaggag caacgacaac
     3181 ttatgataat ccttttatga aagcaaagga aacaatcgct ttttacgaga aaatttggga
     3241 taaacgattt ctgacatttt tggagcaata agttgtttct tcgtttaaaa aaagcagaat
     3301 ggatactggt ttatccattc tgctttatta cgtaaaatga gaaaataatc ctgttctatt
     3361 tttatgggat aaaccattta ttttttgtat aaaaaatgtt tgcgtttaca tataattctc
     3421 ctagaaacat aaaaaggagt gaatagcttg agaacaaaac aagtattaaa gggagcgttg
     3481 gtcatcttat ttgctgtaag tacaagcgtc ccgcaatgga cagtatatgg agagagcatt
     3541 tcttccgaat cggaatctcg ggaggtagaa accacagaaa ctactgatgc tggaaatgaa
     3601 acgaaatcga gtacttcaca aacagaaaca aatactgaaa caacggttga gacatctgtt
     3661 actagcacag atcaagaaac gaccgctacc acagataacc aacaaacaga agaagcaaca
     3721 gctacaaaaa tgtcaaacga cgaatcctct tctgctaaag taagtgacaa agagaatggg
     3781 acagaaaatg atgatatcgg taaaggtgaa aatttcaatg gtaaagaata tttgaattac
     3841 ttcggtaaac agatgacaga aatacttaat ggaccaacag aagacatcaa gtcaaaatta
     3901 atagagttat tagtcactga gcttgctgtt aatgattatg gtgtagataa agagattatc
     3961 aaacatctga ttgctactta tctattgtac gatatcgctt ttgcaaaaaa cagtgtgttg
     4021 atgtcgatgc ttggaggtac agatgatggg acaacgccta cagaaaacac aggcttctat
     4081 ccaaatacaa aacaacttca tcaaactgta ctaaacaaac aagatggcaa aatgatggat
     4141 gtatatgcat attatgtcga tcaaggttca gataaaacag tcatgatcca tggcggattt
     4201 cgagggaatt ggaataacgg gatcgttaca gaagaataca atgattttta taaggccggg
     4261 tataatttat tatttgttga ttccagagcg actggtaata gcggaggcga ttatgtgacc
     4321 tatggacaat atgagtcaga tgacgtcctt tactggataa atcaagaagt tcgagagcgg
     4381 ccatctcaaa agattttact ttatggtggt tcgatgggag cagctacgat gatgtctgta
     4441 ttagcgaaag atattcctgt caatgtcaaa ggaattattg aaaattgcgg atttgcatca
     4501 attgatgaac aactacgttt tacgtacagc cagacagtag cccccgcact accggatgcc
     4561 ataaaaaatc agttagacat cattggggat caagagcatg aagatttatt tatgggcctt
     4621 ttgaaacaat actattttga tcaggagatg catttggata caaaagctgc tctacctacg
     4681 attggaatgt cagattcatt gcctaaattg attatccatg gaacagcaga tgatgtagta
     4741 cctgtgtcta acgcgcaaaa attatatgag ttatcaggag ggtataaaga tcttttatta
     4801 gtagaaggag cagggcatgg aaaggcacag gaagttgacc atgctgccta cacgaaacat
     4861 gtaacggact ttttgaaagt agtgttccat gatcagataa acgtaaagta tgtggatgaa
     4921 aacaataaaa gtctactcaa agaccaggac gaaatccagc tttacggagc ttatggagaa
     4981 aattatatga cagagcaaaa gacattcgaa ggatacgaat tagcaaatgt tgaaggaccg
     5041 actgaaggga tttttaatga gacgatacca actattattt tctattacaa aaaaatccca
     5101 gtagctccac caaaaaagca agatccgaca gagaatgcag acaataaggg aaagaatgaa
     5161 cctcgagagg atcaatcaag tgatgatacg caaccgattc agatgacagt gaaagtacaa
     5221 aaaaagacag atgcaaaaaa aagaatgttg ccgaaaactg gagaaaagaa acattcgctt
     5281 ctcatttatt caggtggatt tttatctgtt agcacgatta tcacatggat ttggcgaaga
     5341 aagagagggt aaatgaatga aatggttcaa gcgaatagtg attgcagtac cagttatcat
     5401 cgtagttatt ttagtcactg tatttttgat taatcaaatc acaccaaagc cgattagctt
     5461 gcttgttaga aaacaatttg acacaagtga aaaagaacaa gtatatgccc gaccagatga
     5521 tttccagcaa aaaatcgaac aaatcgatat aaaaaaagaa atatcctatc cttcaaagta
     5581 caaaaataat aatatggata tctacacacc gaaagtggaa aagaaaaaat taccgattct
     5641 tttctggatg catggaggcg catatgtagg aggggataaa aatgattgtc gtgattattt
     5701 ggaatatcta tgttcagaca cgcagcaaat catcgtaaat atagattatg aacgatcacc
     5761 ggaagcaaaa catcctacac cagttatcca attgaacgaa gccattcaag cagctaaaaa
     5821 agagataaaa gatcaagcag attggtcgaa tatttcgatt ggtggaaatt cggcaggtgc
     5881 acaaattagt ggagaatatt tgttagcact tcaaaatgaa gaaattaaaa aagctgatca
     5941 tcttgatccg actttggaga acaagcagat tacgaagttt atttctttgt ctggtctgtt
     6001 ggatccttct gcacttactc aagtaagtga taaaatctct agctttcttt atgaaaaatg
     6061 tggatgggct tactttgata ataaggactt tgaacaatca gagaaggtca aaagtttagc
     6121 cttggtcaga catgcggatc gttggacgca aaacacattc ttaacggatg gaaatacaaa
     6181 tacgtttacg aaacaaatgg aagaagtaac ttctgtattt gagaaagcgg gggtgaaagt
     6241 cacaaaagta gattatgcgg agaaaaatgc tgttttgaac catgaatatc agtttgattt
     6301 cagcaataaa caagcgcaag aaacatatca aaagttagta atgtttttca aggagtaaag
     6361 tcttatgatg caaaagaggt tgtaatctgt cacttagctc caagtcacaa ggagcaattt
     6421 caataaacaa gcttttcatg gtttatgaat attgtaactt gtgactgagg agttagagat
     6481 ggcaaggggc accttttatt cgaaatttgg gaatatcaca cgattattgt cacgatctct
     6541 tttacgtaaa ttttataagt cagatttgtt gttttgatcg tttaaaaaaa gcaacaaatc
     6601 cgaagcctgc ggaggcaaga agggacaaaa agccgagtgg taagcaaaag aatgctggtt
     6661 ccactaatat tccgttgtta ttgatataag gctgggaaca aagatgaata gcaaataaaa
     6721 tcaatgcact agcgaaaaaa agcaaagaaa tatggatagc tttcataagc agcacctcct
     6781 tttttataat tgtatgataa atagattaag aatctataaa tgtgacaagt ttgttaggat
     6841 gtgaataaaa tgatcatcta gcgaacttga aacaaagatg gctaatgata agcaattaat
     6901 gattcagcaa tcagataggt acaaagttta ccatcatacg tctagaccaa taatagaaaa
     6961 tatgggaata taatagaaaa gatttaggat acccccaaaa gaatttcccc aaaaattcga
     7021 tgtatcctaa acttttttat tttacataac aatcatatca actgtgaaat aaaattacaa
     7081 atgataacct aaaatgaggc caaaagccag tgttatcaag gaaatactta attcaccaaa
     7141 atttttttta attttttaga aaataagaaa caaaaataat aaaaaaaaga aaagataaat
     7201 tttttactac tgtatactaa ctttgttatc gcatctaaca agtaactcga ataataccaa
     7261 tacaaccttc tccaaagatg cgatgacata cgttctaacc caccgactat tgatgttctg
     7321 taccaccctt gcagaaccaa aaaagccact accaaatatt tggtagtggc tttttttgtt
     7381 ttatggattt ttcgcttttt ctgttatggt gctattcttt gatttcagaa atagtatgtc
     7441 gaactttagc gatagaacga cgtgattctt tttcgttgta aaacatcaaa atcacatata
     7501 gagagtccaa aatgcttagt tgagaaatac gggaagctaa agcttcagat cggaattctg
     7561 tttcttcaga aatagaaata aaaacgacat cgccaagttt agcaagggaa gaattagctt
     7621 ggctagtaat aacgattgtt tttgctccag tttttcttac ggtttcagca atatggattg
     7681 tttcttttga ttgccctgtg tgagaaatca aaacagcaca gtctttttcc gtcaataaag
     7741 aagcttccat caattgaata tggtaatccg tgctgtgaga agttgcgata ggggaacgca
     7801 aaaatttatg ataagcgtca gttgctacaa tctctgaacc gccaacaccg aaaaaataga
     7861 cacgtttaga ttcattgata atagtagccg ctttttttag atccttttgt tgtaacaact
     7921 tttttgtgtc atttaaagtt gaaatactag actcgaacac cttttgcgcc atagtgagct
     7981 cagtatctgt tttctgaata ttttcgtgga tagaaatagc agatagatca ttttcttgga
     8041 tcaacatggc cattttaaaa tctttaaatc cattataacc aagttttttt gtaaattgaa
     8101 aaaaagtaga gtcagcaatg cccagttcac ttgctaaatt gctaatagga ctatgactta
     8161 ctttgcttgg attttctaaa atataatcgg cgatagcctg ttcttttggg ctgaagtcgt
     8221 tgtacattgt tttgatttgt agtcttatgg agacgctcat gagaattcct cattttcata
     8281 ataagattat cattttttcc gttctcatta atgttaaaga tacaaggaga gtaggaaaaa
     8341 atcaaacaaa aaatattttt gtaaaatcta tttacaaaaa aatttttttg tataatgagg
     8401 gtataagaaa tggaggcgct atcatggaaa ttggtgtaat tggtttggga aaaatgggat
     8461 tgaatttagc tttaaactta aaagagcacc atcacaaagt acaaggactc gacatttcaa
     8521 gtacagctgt tagtcaggca agagaaaatg acattcctgc ttatcaagag atctctgaat
     8581 ttctagctac ttttgagcag aaaaaaatca tctggttaat gcttcctgct ggagagataa
     8641 ccgaaaacat gttaagaaaa ctttttccaa aactcggacc aggagacatt attattgaag
     8701 gcggaaactc ttattataaa gattcaatcc gtcgagcaga agagtttcag aaaaatgaaa
     8761 tcggttactt ggattgtggg acttctggag gaatcgaagg agcacgacat caagcctgct
     8821 taatgattgg aggagataaa gaaacttttc tatccgtgga acaattattc aaagatgtag
     8881 caattgaaag aggatatctg tatgctggtg catccggaag cggacacttc ttgaaaatga
     8941 tccataatgg aatcgaatat ggaatgatgc aagcaatcgg tgaaggattc caacttgtac
     9001 aaaaaagtga gtatgctttt gacttggaac aagtcgcacg tgtttggaat catggttcgg
     9061 tgattcgcgg ctggctgatg gaaatcatgg aagatcagtt aaacgaacat cccaatttaa
     9121 gtaattatcg gggaatcgtt tctgcatcag gtgaagcaaa atggacggtt gaaacagcgc
     9181 tagaaatggc tgttccagtt ccagtaattg caactagttt gttcatgagg aatctttcac
     9241 aggaagaaga tagtttttca gcaaaagtag tttcagcact aagaaatggg tttggcggac
     9301 atgaaatagt tgaaaagggt gagtaaaatg ccagattatc atatagcaat cgttaattcc
     9361 agcagtttcg gaagagtctt tccacaccac ttggaaagat taaagtcagt tggagacgtt
     9421 catttcttta ctgttgatca agagattcca ggaaaagaac tagcggagtt acttaaagga
     9481 ttcaatatca ttattgcaag tgttacgcca ttttttcgac gagatttttt tgaaaacaaa
     9541 gatgaactgc tattgatttc ccgacatggt attggttaca acaatattga tttaaaagca
     9601 gcaaaagaac accagacaat cgtatctatc atacctgctt taattgaacg aaatgctgta
     9661 gcagaaaata atataactaa tttgttgtcc atgcttcgac gcacgacaca agcacaagca
     9721 agagtcaagg acgatcgatg ggaagatcgg gcggagttcg tcggtaggac gctgtttaac
     9781 aaaacagttg gtgtgattgg tgcaggaaat accggaagcg gagtaatcga aattttgcgt
     9841 aacggttttc gctgtgatgt tctagcttat gatccttaca aatcagattt atacatcgaa
     9901 agctttggtg caaaaaaagt agaacttcaa gaattactgg aacaatcaga tatcatttgt
     9961 ttatgtgcga atttgacaga agagaactat cacatgattt ctgataaaga agtcgatcag
    10021 atgaaagaaa atgtttatct atctaatagt gcaagaggcg ctctagttga tgaagaagcg
    10081 atgattaatg gtctgaaaag cggaaagatc gcaggatatg ccacagatgt tttagaagaa
    10141 gaaccgggac gtcaagatca tccgtattta gcgtttgaac aagtagtgat tacgccacat
    10201 atatcggcct acacaaggga atgtttagaa gcaatgggtg aaaaatgtgt cagagatgtc
    10261 gaacagatca gtcagcacct tcttcctgaa cgttctgtcc agacaaagag caactatata
    10321 aaaggatgat gaagaaaatg gtgaacggtt taaaagtgaa aacaggccct caattttatt
    10381 tatatgaaga aggtggtatt tcaaaagttt ctgatctctt gaaatcatac ggcgcgaaaa
    10441 gagtactggt cacccacgga acggtatcat gggaaaaggc gctacctaaa ttagtatttt
    10501 tgaatgacga aacgattcaa tttttttatc atcgttattc tggagaatgt agttatgctg
    10561 aagccagacg aatagctaca atcatcaaaa aaatgaaatc gatttcgtag ttggcgttgg
    10621 cggaggaaaa ctgaccgatc tagtgggtta tagtgccaat cttgcaagaa tcgatttcgg
    10681 agtcatccct actttagcta gtaattgtgc tccatggaca cctcttgctg tcatgtataa
    10741 ggagaatgga caagcagaag ggaaaacaga acacttttac agacaagctg ctttttttat
    10801 aacagatccc caattagtga ttgattcccc tgttccatat ttcattgccg ggttagcaga
    10861 tacactagca aaatggtatg aatctgagct catcttagaa caagattttt tgcaagatga
    10921 atcatttttg aagctagcac aagatactgc gaagatctgc aaagaagaga ttttgaatca
    10981 ttctgctaaa gcaatccaag atatggcaca aagaaaactg acatcggagt tcaaacagct
    11041 ttcagagatt atctttgctg ttgctggttt agttggagga ttaggagata aatacgcaag
    11101 aaatgcggca gcacatgcta tgcatgacgc tatcagtaaa tatattccgg aaagccatcg
    11161 ttttctacat ggagaaaaag tagcgtatgg gattttctat caactggcac tagaaggtcg
    11221 ctgggaaaaa atagacgagt tatatccttt gtatcgggaa ttagaactgc cggtttctct
    11281 agaacaaatg ggattatttc cgaaagataa acagataata gataaaatgg ctgaatttat
    11341 tgattcgaaa gagaaagtgc atttgattcc tatagaggta acacagaaat cactgataca
    11401 gtcaatgatg gaactggaag gttatatcag aaagatctga aggaggagga cacatggaaa
    11461 cattaagtgt agtacaaagt ttgttgattg ctttatgggt agccgcggtt atgtcccgct
    11521 ggttaggtgg aggagccacg ttgaccttac gtttttctcc tttgatgact ggattagtcg
    11581 tcgggttgat cgtgggggaa gttgcacaag cgatgattgt tacagccgct ttgcaaatga
    11641 tttatatggg ggttttttct ccaggaggat caatgccagc tgaaccatct attgcagcag
    11701 ctatcgcagt accagtcgca ttattagggg atctgtcgcc agaagcagcg attgccgttg
    11761 cggtgccagt cggattgctc ggaagttact tgtatcagtt tcgttttttc atcaatactt
    11821 ttttaggtaa atatacagac aaagctgtag aagatttgaa tacaaaagct atggcccgat
    11881 caattattct ttatccaacg gttgcctcat ttttattatt cgttccgctt gttttcattg
    11941 ctttatattt aggggcgcca ttgatcgctg atgtaatcaa agctttagag ggaacagtcg
    12001 ttatccacat tcttgaagta gtaggtggag ggttagcggc aatcggtatc gcgacaactg
    12061 tatacgttat cggtcgaaaa gattatctgg tctttttctt ccttgcttat tttatgagtg
    12121 ttgttttgaa atctttagat attacgatgg ttacttatgc catttttggg attattatcg
    12181 ctttgatctt tgtccagtcg caaaaaggga aacaagtaca gcctgccaca agcaataaca
    12241 attcagcatc ttttgatgac gaagatgact atgatgacgg attttaattt aggaggaatg
    12301 ggaaatggct gaaataatgg ataatcaaac aggtgctcct gaagaaatca cgaaaaaaga
    12361 tgtaacaaaa gcctatctga gatggcattt tgcaaacgaa attcctcact cttttgaacg
    12421 ttacttggcg ccatcactac tatacggaat gatgccaatc ttgaaaaaac tttataaaga
    12481 tgaagactct ttaaaagcag catataaacg gcagttactg ttcttcaaca ctcaattaag
    12541 ctggggtggc ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa gagcagaaga
    12601 agagtatgaa gaaaaagaga tcttgatgca ggatgacttg atgtataaca cgaaagcggg
    12661 attgatgggg gcactagcag gaatcggaga tgcaatcgat tcaggaaccg tccaatatat
    12721 ttttatcgca atcgcggtac cttgggcaca acagggaagt cctttaggcg cactttttcc
    12781 ttttatctgc tttgggttgt atcaggtttt actaggggta tttttcgcaa gacaagcttt
    12841 cactatgggc cgtaatgcta caggcctaat gcaaaacact ggtgttcaga cagcgattga
    12901 aatgctatca gttttagggt tgtttatgat gggggtgctt gcgggaaact atgtaaaagt
    12961 agaatcttca ttgaaatttg cgatatccgg acgtgagttt gttatccagg aaattcttga
    13021 tcaaatcgtg ccagggatat tacctttagc tgtcgtaatg ggtgtgtact tgttctatac
    13081 gaaaaaagga ttgaaagtca cacaagcatt attatggctg actggaatat taattgtgct
    13141 agcaggaatc ggtattttat aaaaagaagg gaacgtgaat aagatgggtg tagtcaattt
    13201 agcgcgtgtg gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat ctcaaaaaag
    13261 tggagtgaat tccatttttg ttgtagacga agttgtagca aaagataaat ttatgagaga
    13321 tctttacaaa aatgcaggaa gccgtacagg acaaaaaaca attgtcatta caccagagaa
    13381 agctaaattc tattgggatg aatatcaatt taaagaatac aattgtattt tgatcgcaaa
    13441 aacagtccaa acgatttacg acttagtaaa acacggaatc cccatggaag aattgaatat
    13501 tggagggatt gctcaaaaaa atcctgatac agatctttta gtgacgaaat ctgtttattt
    13561 aaataaatca gatgctgaaa aattgaaaga aatgaatgaa caatatggcg taaagaatat
    13621 ttattttcaa gccactccat ctgcacctaa aacaggatta aaagatgtat tgaacaagtt
    13681 tgatttgtca taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa tgaagaagaa
    13741 cgagctgttt cgtgattggg aatttcgtta tcgctatatt taccgtaagc gccgtacaaa
    13801 aaagagtaaa cagcgttttc tttcagcatt agtatcagat atttacagta tgagaacaga
    13861 tgtgacagtc attgcttacg atacgctagc ctaccgatca aaaaatattt atgttggaga
    13921 tatcgaaaaa gcagagaaag ttatctgtac ttattacgat acacccgttc atgcattagg
    13981 aagttatttc atgtttgatt ggaaagacca aaggaaaaaa acaatctact ctatattatt
    14041 gtccttcatt ttgttgttca gtttaggttg gtggggaatg atgatatata acaaaaatcc
    14101 tcatcacgta ttcgatctgc tatccgtata aacaggtatc actgtactag catttgggag
    14161 ctactttttc ttattgggaa aagcagctcg cggatggcct agcagacaaa cttttattcg
    14221 aaatacttca tccatattaa cgatgctaga aatgatccgt acgattgatg atccgagtgt
    14281 agcgtatgcg tttgttgatg aaggctgtta tggagaaaag ggcttagaca gcgtgagatc
    14341 gggtatgaaa aaagaggcta ttctttttta tctagactct gttggggcag atacaccgct
    14401 acagttttcg ggaaattatt tttcaaataa agaacagtgg ctgaaacaag ttgataagtt
    14461 gaaagaaaaa aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc agttcttcta
    14521 tttgaccaaa acagatttac gagggaaaac atttaattgg caaaatgcca atcagattat
    14581 tgctcttttt cgataagtaa aaagtgcaaa acaaggagtg agaagactaa tgttggaaat
    14641 catgatcgtt acacatggta agttgagcga aggactgaaa gatgcggcgg aagtaattgt
    14701 cgggacatcg agccatattg aaacaatgtg cttagagtct ggggaggaca tccaaacatt
    14761 cggagagaag gtcaaagaaa aattaactat gatcaacaca gcagaaccca tgctagtatt
    14821 tgttgatcta attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca atgaactgac
    14881 tgaagaaaag aaagcagcca cttatgtcat agggggcgtg aatctgccaa tgttgttaga
    14941 agcaatcaat catcagatgc ttcacacacc gatagaagaa gcggtggaac aaattgtcaa
    15001 acaaggaaaa gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa aattagatga
    15061 ggacgatttt tagaaggaag gctgaaaaga tgaagtgggg atttagatgg tatggtgaaa
    15121 aaggggattc gatcccatta aaatatgttc gccaaatacc gggaataaat gggataattg
    15181 gaactttgct gactaaaatg cctggagatg tttgggagat ttcagaaatt gaggagttga
    15241 aagcatctgt tgagagccaa ggactggaac tgcttggaat tgaaagtgta gcgatccacg
    15301 atgcaatcaa ggcagggaca gatgaacgag attactatat tgataactat atacgaacaa
    15361 tcaaaaactt gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa ccaatttttg
    15421 gatgggcgaa aactgatttg tttaatgaaa acaacgatgg aagctattct cttttatacg
    15481 atcagaaaat cgtcgatcag atggagccag gagaaatgta tcaattgata cacagccagt
    15541 caaagggatt caaattgccg ggttgggaag aagaacggtt gaaaaaattc gctcagctgg
    15601 aaaaactgta tgaaggaatg accgaagaag aattatttga aaatctgact tattttttag
    15661 aaaaagtgat tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat ccagatgatc
    15721 cgccatggga actttttggt tttcctagag tgacgaaaaa tcttggcgac ttgaagaaaa
    15781 ttctaaatgc agtagattct ccatataatg gactaacgct gtgcacagga tctttagggg
    15841 caaatccgga aaacgattta gttgaaatca tccatgaggt aggttcgcgc atccattttg
    15901 tccacttcag aaacgtggag tttttaggag aaagaaagtt caaagaaacg gcacatccct
    15961 ctacagaagg ctcccttgat atgtatcata ttatgaaagc gttggtgcaa gaagggttcg
    16021 acggtcctat acgccctgat catggtagaa ccgtgtgggg tgaagtggct atgcctggat
    16081 atggcctgta tgatcgtgca atgggactca cttacatgca aggtctatat gaggcgataa
    16141 gtaaggaaaa accactggga gagcattatg aataaaatca agctcttcat ctttgatatg
    16201 gatggccttt tattagaaac tggacgttta gcttatcgtg cttatgtaaa atcagcacaa
    16261 aagtatgatt atgaaatgcg taaggaagtc tattatttac taactggcca aacagaaatg
    16321 gcgataagag aacaaatggg cattttgtac ggagaagatg ttccatatat caaatggcga
    16381 gaagcaatca atcaatataa agaaaaaatc gtgaaagaag ataagagagt atatacgaaa
    16441 aaaggagcag aagaaatttt atcttttgca aaagaaagag gaatccatac aatcgtggct
    16501 tcgtctaata cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct atatgccttg
    16561 tttgatcaaa tcatttcagg ggatgatgta aaaaaaggaa agccggaacc agagatcttt
    16621 ttgaaagctt gttcaaaaat gaatattcct ccaagtgaag cacttgtatt tgaagactcg
    16681 attgcaggga tcgaagcagc aaggcgagcg ggcattttgt cttttctcat caaagatcat
    16741 ttgatagggt tgcccgatca taatgggaag cataagttga aagtagatgt aagtttatat
    16801 gaatccaata aagcagacta tgaattttat gacctgcatc aagcaaaacg atttttagaa
    16861 agtaaagggt tgtatctcta ataaagagga ctattttcta gtcctcttta tttattgtcc
    16921 tgcacgactg agggaacaga tgaaaaagaa gtaatttttt attttgaagt atctacctta
    16981 tttgtagtga acaatttaga acaaacacaa aacgcatcta ctgttatcta ccagtagatg
    17041 cgtttaaaga gaagaacgag aatcgtccat atgagggttt ccactcctta tgcagatttt
    17101 tttaattctt gcactgcttg ttcgatcgta acacctgtcg ctacatgatt ggtattttct
    17161 ttgtcaaaaa cgacaaatag attcaaatct gtatccaagg ttactcgata ttgtatatct
    17221 ttgttataga atgtcatagc cattctcctt tctttaaata cttgctgaaa ggcaacgaac
    17281 gcgttgataa cgaaattcca tttataagta taccaccaat tgaaaggttt tcatagttaa
    17341 gaaattttaa agtcaaaagt tgcagataaa agcaaataaa caagaaaaca aaaagtgaat
    17401 caatcgcggt tattcaactt tttcttcgat tcgaagacaa tagaagacaa tgaattttat
    17461 atttcattgc attatccttg tgttattaga aaaagcatga tatattaaat gagtaatcta
    17521 ttttgaaacg taatttgagc gatatatcta catagataaa actccttttg cctatcaaat
    17581 atttattgca acgaaaaaac gtacattgat acgtatcagg aggaatttat ttatgaataa
    17641 cggtacagta aaatggttca acgcagacaa aggttttggc tttatcactg gtgaagatgg
    17701 aaatgatgta tttgcacatt tttcagctat ccaaggtgac ggcttcaaaa ctttagaaga
    17761 aggccaagca gtgtcttacg atgttgaaga tggtcaacgt ggccctcaag caacaaacat
    17821 tgttaaagct taataaaaat tgacttttaa ccgtcttttc gaaagaaaag acggtttttt
    17881 tgtacgaaaa aattcattgg tcgttgaatg ggctgaatag tagtgtataa aacaattttg
    17941 tggagcgtga agcttttatg gtagaggaaa atacgaataa agtgatgttg ataaaaagat
    18001 tcctatcttg aatatgaatc gagatgtctt gaaaatgaat gagtttgacc aatcattatc
    18061 ctatcgaaat ttacagtttc gttcgattgg tctgtcaatt aatgaaaaag taaaaaagaa
    18121 tatgttctag gaagagggcg gcacatctaa atttttaagt aacggacagt tcaaatatca
    18181 agcattgaaa gaggctatcc tactgtttga aggaatacga atatataaaa acgacaaaga
    18241 ggaaaaagta gttcattaaa tcccttcaag tgatgaaaga atgagatcta ataaaacctg
    18301 atctttcatc tgttttttta ataacgtaat aattttttta ttttttgtgc tttcgccgtt
    18361 attgtagagt gatttagcat tcttattttt aaaaataact gtgttatata ttaaaaaagg
    18421 cataatagtt ataagctttt ttaaagaata tttagaaatt attaattttc gtctgaaaac
    18481 aaaaaaagaa agcgttatta ttttagtcta ttttcagggg gggaacctta tgataaaaaa
    18541 actattttta ctatcaatat taacgctagc aatggcaggt tgttctgact ctggcttagc
    18601 aactgaactg atcacaaaat caactgaagc cacgacagag gagttaaaat gtggaaacga
    18661 tcacagtgac tacagaggct taaatcgacg atgcatggga atattaggca gaagcaattg
    18721 taagcatcga tgtagtaatc gaagaaggtg tagaagaagc aacagaaaaa tcaaaagaaa
    18781 gatcaaaaga agcaatctgt gctgtgcatc gtatacagac gtcgaagatg cggtactttt
    18841 aaaccacgtc aggagatgtt catttatgtc tagggaagtt ggcgatgaac caaaactaga
    18901 gtacgctttt agttttgcac gtgaggatga agataaccct aaaacttacg acattttagc
    18961 ttgatctaag atggttaaag ctttttaatg aagtaagagg agatagaagg cttttctagt
    19021 tatattaggt aagataaatt cctttttgct ccaaatttgc taaacattct gctagataaa
    19081 cttcatcgtg ttccggataa atcggcacgt tgcgctctac agtaggtaaa tcaaaaaatg
    19141 cttcgcatga tttgccacga tacataggat ttttccactc tggtgctggg ctgtaaccac
    19201 gtttttccat ttcatccatg acaagctgat gatattggaa taatctataa ggagaatagt
    19261 gaaaaacata atccacagta gcatgtttct ttccccagcc atttcctctt aatgcacagc
    19321 attcccgatg ttgtcctaaa agctgttgac gaggaagctt tgaaatcaat ttttcatgcc
    19381 ataatcgcat agaaatgtct cctttatata tagattgtta accttagacg ttaaatgaaa
    19441 aaaccagcac caaggctgga aaaaatcact tcagtttgct tgctgtttct gagcgaccaa
    19501 ttaggtaatc aacggaaata tcgtaaaagt cagcgatata gattaattca atgatgcttg
    19561 gaatttcttc tccttcttcg tacttacgat atttgaataa aggaatactt aacgcgctag
    19621 aaacagctac ttgagataaa gatcggccag ttcttaagaa attcagacga caacgtaaag
    19681 tatccagtaa aatagcttct tcgatactca tttaatcacc tttcattata gtagaatagt
    19741 tataatgact atagaaagct acacatgcaa tcaatagtac tcaatgtttt ttatttaaac
    19801 gtatcaaata aagaatgacg atcaataaag acaaaaccag tcctgataat tcttctataa
    19861 aaacactatc gggttcaaag tagttgtaat taaaaacttt ccacataaag tagatagccg
    19921 gaagagtcaa aatagctaaa taaaaccgtt ctttgttcca attcataaga taaacaatac
    19981 ttgcgaccag tgcgaccgca ggcacccaaa acaaaactct caccaacgta atcatgtaac
    20041 ggactcctca acgattcata taataaaaga taacatgtta ttgcatacat cacaaatgaa
    20101 atgctaggaa aataaaagaa gaatagatca atgaaaaatg aaaaagcaga acagttttct
    20161 gctgataagc aggaactgtt ccgctgaatg tgtcttatct atcgagaaaa aatcaatgga
    20221 ttgatatcta agtaattcaa tacatataaa aagaggacga tcgcattcac taaaaaggaa
    20281 agaatgatga tcccatagcg aatggaaaca tatttaacaa atacttttgt ttgaatacgg
    20341 gcatatcttt ttcctttata gctgagcatc atgacaagaa gcaaaagggt aatcacacct
    20401 tgaaaaatca ttccaataaa cagaattcct gaggtttgat aatttaattg gagaaaataa
    20461 gctgctgccg caactaaatc gaataaaatc gcaataaatg gcaaaataat cactcctaac
    20521 attgagaata aataaaagaa taacattttt tctaaaataa gcaaataaaa attaaagtaa
    20581 caaaatagtt atgacacaag taaaagaaat aagaataaca atattatttt acttatattt
    20641 tgaaacttgt gagagtttgt cattcgtgct atagttgaaa agtaacaaaa ataaatttta
    20701 tttatgatg
//
LOCUS       DBNICI010000036        20005 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.036, whole genome shotgun sequence.
ACCESSION   DBNICI010000036 DBNICI010000000
VERSION     DBNICI010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.036"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..1014)
                     /gene="tsaD"
                     /locus_tag="AC1ZCC_001074"
     CDS             complement(<1..1014)
                     /gene="tsaD"
                     /locus_tag="AC1ZCC_001074"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIQ1629341.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1011..1475)
                     /gene="rimI"
                     /locus_tag="AC1ZCC_001075"
     CDS             complement(1011..1475)
                     /gene="rimI"
                     /locus_tag="AC1ZCC_001075"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIQ1629342.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(1482..2024)
                     /gene="rimI"
                     /locus_tag="AC1ZCC_001076"
     CDS             complement(1482..2024)
                     /gene="rimI"
                     /locus_tag="AC1ZCC_001076"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIQ1629343.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2008..2730)
                     /gene="tsaB"
                     /locus_tag="AC1ZCC_001077"
     CDS             complement(2008..2730)
                     /gene="tsaB"
                     /locus_tag="AC1ZCC_001077"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIQ1629344.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(2921..3901)
                     /locus_tag="AC1ZCC_001078"
     CDS             complement(2921..3901)
                     /locus_tag="AC1ZCC_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIQ1629345.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(3925..5409)
                     /gene="galT"
                     /locus_tag="AC1ZCC_001079"
     CDS             complement(3925..5409)
                     /gene="galT"
                     /locus_tag="AC1ZCC_001079"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HIQ1629346.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(5421..6410)
                     /gene="galE"
                     /locus_tag="AC1ZCC_001080"
     CDS             complement(5421..6410)
                     /gene="galE"
                     /locus_tag="AC1ZCC_001080"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HIQ1629347.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            6658..6825
                     /locus_tag="AC1ZCC_001081"
     CDS             6658..6825
                     /locus_tag="AC1ZCC_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HIQ1629348.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(6887..8698)
                     /locus_tag="AC1ZCC_001082"
     CDS             complement(6887..8698)
                     /locus_tag="AC1ZCC_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HIQ1629349.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(8695..9060)
                     /locus_tag="AC1ZCC_001083"
     CDS             complement(8695..9060)
                     /locus_tag="AC1ZCC_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HIQ1629350.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(9223..9618)
                     /locus_tag="AC1ZCC_001084"
     CDS             complement(9223..9618)
                     /locus_tag="AC1ZCC_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIQ1629351.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(9636..10598)
                     /locus_tag="AC1ZCC_001085"
     CDS             complement(9636..10598)
                     /locus_tag="AC1ZCC_001085"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HIQ1629352.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(10598..10810)
                     /locus_tag="AC1ZCC_001086"
     CDS             complement(10598..10810)
                     /locus_tag="AC1ZCC_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HIQ1629353.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(10831..11529)
                     /locus_tag="AC1ZCC_001087"
     CDS             complement(10831..11529)
                     /locus_tag="AC1ZCC_001087"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIQ1629354.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(11549..12091)
                     /locus_tag="AC1ZCC_001088"
     CDS             complement(11549..12091)
                     /locus_tag="AC1ZCC_001088"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIQ1629355.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(12223..13227)
                     /locus_tag="AC1ZCC_001089"
     CDS             complement(12223..13227)
                     /locus_tag="AC1ZCC_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIQ1629356.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(13224..14213)
                     /locus_tag="AC1ZCC_001090"
     CDS             complement(13224..14213)
                     /locus_tag="AC1ZCC_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIQ1629357.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(14210..15016)
                     /locus_tag="AC1ZCC_001091"
     CDS             complement(14210..15016)
                     /locus_tag="AC1ZCC_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1629358.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            15182..16138
                     /locus_tag="AC1ZCC_001092"
     CDS             15182..16138
                     /locus_tag="AC1ZCC_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIQ1629359.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(16215..16733)
                     /locus_tag="AC1ZCC_001093"
     CDS             complement(16215..16733)
                     /locus_tag="AC1ZCC_001093"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HIQ1629360.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(16821..16985)
                     /gene="rpmG"
                     /locus_tag="AC1ZCC_001094"
     CDS             complement(16821..16985)
                     /gene="rpmG"
                     /locus_tag="AC1ZCC_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009554760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIQ1629361.1"
                     /translation="MGGKNMRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRE
                     RKVTLHRETK"
     gene            17198..17644
                     /locus_tag="AC1ZCC_001095"
     CDS             17198..17644
                     /locus_tag="AC1ZCC_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HIQ1629362.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(17839..19734)
                     /gene="asnB"
                     /locus_tag="AC1ZCC_001096"
     CDS             complement(17839..19734)
                     /gene="asnB"
                     /locus_tag="AC1ZCC_001096"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIQ1629363.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         5954 a         4293 c         3444 g         6314 t
ORIGIN      
        1 ttcagataaa accaagcttg gatcagcatt caactgatag ctagcaaaat ttcttttttg
       61 catttccaca tgtgctgctg ctccaatcat tgcagcatta tctccacaaa gtcgaagagg
      121 gggaatcact agttctacct cagggagttt agctgccaaa gcatcctgca atccttcacg
      181 caacccttga ttggccgcta cacctccagc tataatcagt tgcttgactg gataattttg
      241 acaagctcga agtgttttag taatcaatac atcgatcaca cttgcttgaa agctagctgc
      301 taaatcattt ttgtctagat cttctccacg ctgttgtgca ttatgcacca aattgataaa
      361 tgcacttttt aatccgctaa aactgaaatc ataattatct tcatggatca tcgctcgcgg
      421 aaaatggtaa ttatcttttc cctgatgagc tagctgatcg atttcttttc cgcttgggta
      481 agaaagacct aatactcttc caactttgtc ataagcttct cctgctgcgt catctcttgt
      541 ctccccaatg atttcatatg atccatcttc ttgcatatag actaattctg tatgtcctcc
      601 gctaacaaga agtgccatca atggaaactg gaatggttta accagacggg ctgcatagat
      661 atgccctgcc atatggttta caggaataag cggcaattgg tgtgcccaag caaaagcctt
      721 agctgcagaa ataccaatca gcaacgagcc gacaagtcct ggtccataag tgacagcgac
      781 tgccgataaa tcttctgccg acacaccagc ttctaccagt gcatcttcca agcataaggt
      841 gatttgttct acgtgatggc ggctagctac ttcaggtact acgccaccaa aacgtttatg
      901 actgtttatt tgagaagcaa cgatgttaga taagatctct gttccatttc taacgaccgc
      961 tacactcgtt tcatcacaac ttgattcaat cgccagaatc aattcttcat tcatttcatc
     1021 gtctccgttt ttaactttct gctcataatg atcgcatctt ccgtcggatt gcgataatat
     1081 gatttccgtt tacctaatat acgaaacttt tctgattcat aaagttttct tgctgcttca
     1141 ttagatagac gtacttctaa aaaaacatgc agcgaatctt ttatttgttc cgtaatcagt
     1201 aattgtaaca aatgccgtgc atgccctttt ccttgttctg aaactgcaac agcaatattt
     1261 gtgatctcga cttcatctat tactttcgag tatccaataa agccacagat ctggttatct
     1321 tcagctaaaa gcagataatc agagtgaggc tgttggatgt ctgccaaaaa ctgcattttc
     1381 gtccaaggtg aaccatgtgg ataagcattt tcgctaatca tccatacgca ttctgccaag
     1441 tacgcttcat caaaatcttt ttttctgcaa atcaactaat ctcactcatt ttctaaaagt
     1501 tttatcatat ccaacgcatc ttcttttgtt tcagtgtaat atcttttttt gatcgttgtt
     1561 gcttgaaacc ccaatttccg ataaagtctt tgtgcatctt gattactcat acgcacttct
     1621 aatgacatcg tctcacagcg attcattata gcaaagttct cgatttcgtc aataagcagc
     1681 gaaccgattc gtttacgctg atatgccggt cgaacggcaa tattcgtgat atggcaatct
     1741 aaaccgaacg ctcttgaacc gataaaacca atcattcttc cattatcaaa gatacctaga
     1801 tatagatgag gaagcggtga cttcaattct gatataaagg cactttttgt ccatggcaat
     1861 tcgccattat agacttctcg ttcgacatcc agcagctctt tgatatcttc tgtagtcaat
     1921 tctcttagta cgtaacgacg atcgatttgt tcgactgttt tttctgtata aatcatttta
     1981 tcttgaaaaa agcttctggc atacgctcta aattttttca acataacttt cgtctccagg
     2041 tgtgtgcgtc tccaaccatt tttcttctgc ttctactcgt tttaaatagc gaggaagaaa
     2101 agcttgtttc tctgcaactg gttcaactaa agcacctaat tgagccacag ttacaccact
     2161 tggaatatcc catgctggaa ctgtattaat catcgcacta ggcaatattt cctttatttc
     2221 agagaagaat ttgtgacaat cactcccgac gaaataaata gcttcattca gcttttgtag
     2281 ttgtgtgaac aaatcagaag cagaaatatg tacatcttca agtacagttt ctaatctatt
     2341 ttctttccag cggtatgccc cagcgtatac attttttctt cgagcatcaa acatgggcac
     2401 aataatatta tccacaccga tgcaattagc cgcgatggtt ttcagactag atactcctac
     2461 tagctcttta tctaatgtat aagcaatcgt ttttgctgtc gttacaccta tacgaagccc
     2521 tgtataagaa ccgggaccat cagaaaccgc gattcggtca atgtcttttg gacttatttg
     2581 aagatctgca aacaattggt cgattgcagg catcagtgtt acactgtggt tgcgtttgat
     2641 atttgtttgt atttgtccaa gaatttgttc gtcttctacc acaccgatcg ccaaagtttg
     2701 gtttgcagta tctattccta atgtaatcat atatttgatc ctttctgcta tgacattctc
     2761 gccttccatt gtagcatact tttttattct gaggatgaaa ataccaaagg caaaactagt
     2821 acaataatac atgattatct gctggcgggc aaattttgcc ccttcctgaa acacaaaaaa
     2881 gctgcggtat cactccattt tcccacagct tttccatctt ttatttcgtt gattctcgaa
     2941 taatcaactc tgtttccaca ataatctttc ttgaaaccgg tgctttggaa ttacttaatt
     3001 gaagaatcgt ctccactgct agctctccca tccattcggt ataaactttt acagtagaaa
     3061 gcgagggcgt tacatatttc gctacactga catcattgaa gcccatcaca gaaatatctt
     3121 ctggtaccac taaacctttt tcttgaatcg ctttcagtgc gccaatcgct aatgcatcat
     3181 tagctgcaaa aagcgcatca ggaacatgcg gattcgtttt taaatattgc ttcactgctt
     3241 gatatcctga ctcgacagaa aaatctgctt cgataattgg accttcgtcg tagccttttt
     3301 ctgccaaaac ttgttgaaat gccataattc tgggatcttt tagccgctga tggtttttct
     3361 tcgtatattc tgttccagca agcatagcga tatgttgcgc tttatgggag acaaagtgtt
     3421 caatgacttt ttgaacactt gacttaaagt cgacaactag agagtcgatt ccttgagaag
     3481 tgccgtcaga atctaccaaa agcaaggcat tttgggatcc tttcatttct aataactgag
     3541 cttcgtcgaa ttttcctagt gcgatcgttc catctgccgg aagatcacta atggaatccc
     3601 acgtttcttt caataaagta acgtttgctt cttctgcctt tttttcgatt cctaggcgaa
     3661 tagataaata ataaagatct tcaagctctt ccgtatcgtc gtaccactgg atcaggcgaa
     3721 gcgtttgctt agtatttgct gcatttttac gatgtttcgt ataatttaag cgttctgctg
     3781 cttcaaaaat tttttgcttt gtggctaagc caacggaaag atcaggatca tagttgagaa
     3841 ctcttgaaac agtagcagga gaaactcctg ccaactgcgc aatgtcttta attgttgcca
     3901 tttgtctctc ctcctttttt acgtttataa aatcgcagta aatcgttcaa acgctgcctg
     3961 tcctttttca tctcgcttga atactccagc gtcttccagt acacgtgcaa aaacctgacc
     4021 tacagatttt tgtacgacat cggtgaccgt atctttcgtc aaatcaggat gttctttttt
     4081 taattgttct gcccacggac gatgatattc agccatctgg ttttcttctc ctaataagaa
     4141 atgctctact tctactagtt ctggtttcaa acgaggtggt aaaacagcaa gccccattac
     4201 ttcgatcaac ccaatgttct cctttttgat atgttggaca tctttatgtg gatgaaaaat
     4261 cccatcagga tgttcttctg aaacattgtt gtctcgaagg acaaggtcca actcaaagta
     4321 attgtccctt cttcgtgcaa ttggcgtaat cgtatggtgt ggtgtaccat ctgctgaaaa
     4381 agctctgata gatactgttt catccgaata tgctttccat ttttccaata ctgagttagc
     4441 tgcttcaata agcaagtttt tctctgttga ttgcaaacgg atcacagaca tcggccattt
     4501 cacgactcct gcgatgactt gcggataatc ttctagtttg ataagttttt ctatcggtgc
     4561 ttttgccata ggaaattcat gacgtcctgc ctgataatga tcatgagaaa gaatcgatcc
     4621 tccaacaatt ggtaagtcag cattcgaacc aacaaagtaa tgaggcaaga cttcagtaat
     4681 ccgaagcagt ctagcaaatg tttcctttgt gattttcatc gggcggtgtt gttctgataa
     4741 aatgatcgaa tgttcattgt aataagcata aggggaatat tggaatcccc agctttcacc
     4801 gtctagattc attcgaataa tccggtgatt ggtgcgggct ggatagttca agcgacctct
     4861 atatccttcg ttttccatgc aaagcatgca ggtaggatag tctactttta ccgcctcgcg
     4921 ttctgctgcg atttgttttg gatctttttc tggcttagat aaattgattg tgatttccag
     4981 ttcaccatac tccgactctg ccggaaattt gatgtttttt gcgatggctc ttgttttgat
     5041 gtagtcattt ttcttactta aattaaagaa atactcagtt gcttcctctg ggcttttcga
     5101 atagtgctgt gcaaagaatg cattgacgac tgaaggtggc ggtgtgagaa aatccattaa
     5161 ttgcgcttca aaaatctctt gttctgctgg caattctgaa agaacaccat tttctcttgc
     5221 cttagcgaca agctgatcca ataagtctac agattcttcc ttaactggtc ttacttcaat
     5281 gctttctaga gcatcttctc ctaccatccc taaaagacga ttctgtaaat ataagcggtc
     5341 catttccatc catccgccag cttcgatcgc aagtgtggca aaatcacaaa tcgtttgact
     5401 gattgacata tctcattccc ctattcttca taaccatttg gatggctgac atgccaatcc
     5461 catgctgttt tgataatcgc tttgatatct gtgtattcag gtttccagcc cagaatctct
     5521 ctcgcttttt cacttgaagc gactaatcgg ctaggatctc cagctcttct tggtgctatt
     5581 tctgctggaa tttcttttcc tgtcacttct ctagctgctt ctagcatttc cttgactgag
     5641 tatcctttgc tgctgcctaa attgaagaag ttgctttcat ttccttcttt caagtattcc
     5701 aaagctaaaa tatgtgcagc aatcaaatct tctacttgaa cataatctct gatacatgtt
     5761 ccatctggtg tatcataatc gtctccataa acagccaagg cttttctttg accaagagct
     5821 acttgtaaaa tgattgggac aagatgcgtt tctggtgtat gatcttctcc gatagatgca
     5881 tctgcttttg ctccggctac attgaagtag cgcaaagcga catagcgcat gccatatgct
     5941 tggtcacacc acttcatcat tttttccatc atcaatttac tttcaccata cggattttta
     6001 ggattagctg gtgtatcttc tgtgattgga gactctttcg gctctccata agttgctgca
     6061 gtagaagaga atacgatttt attcacgttg aactcgtgca tgacttcaag taagatttgc
     6121 ataccgtata cgttgttgtt gaaatacatc aatggttttt cgacagattc tcctactaaa
     6181 gagcttgcag caaaatggat tactccttca atcggttcct tttcaaatac ggaacgtaga
     6241 aattctttat cccggatgtc tccttcataa aaatgagcat ctggatgtac tgcttgtttg
     6301 tgacctgtga gcaaattatc taccacagct acttgatacc ccttttgaac tagctggtct
     6361 acagcgtgcg aaccgatata accggctccg cctagtacta aaattgtcat aaaaaacact
     6421 cctttgatta gtaaaattgt gtttgttgat tctatataat ttaaaaaacg aagtttactt
     6481 ttatggtgaa aacaaacaaa gtaaaatttc gtaaattcat atggtaaatt tataataaaa
     6541 catttagtaa acattttcaa gggttttttg caaaggttta caaacaaacg taaaactagc
     6601 ctttttttct cttttgttcc ataatagata taacaaataa taaatgaggt ggattcaatg
     6661 aaaaaatttg tatctggaat attagtagga agtctggcaa cagccgctgc cgttgctggt
     6721 ttggttgctt ctgtgaaaaa aacagtgatc gatccaattg acgaaaaaga agcaatgatc
     6781 gaagaaaacc gtaaaaaagc aatgcgtaaa cgcgtttctc gttaataatt atttaaaaat
     6841 acgcatgaag actttctaaa aaacagataa gtcatcatgc gtatttttat ttgtcagtca
     6901 atataagttg tgctatttta tctgcctgtc tcagattcca tggatctgta taaggtctag
     6961 ctactgctgc agttagccaa tccacacctt caacaggtat tccatagcaa taaaagtcag
     7021 actggatttc ccctgaagca tcaatcacgc gattcgtacc tcgctcaaca tctatagctt
     7081 ctgaatgata acttcctgta ttatctgaaa atgaaaaagg gcgaacaagt ttgttttcct
     7141 tcaaattttt tatgacagga ttacttgaac gagaaaagct tgtttgtggc aatcgagctt
     7201 caatcaaata gcgactgctg atttctttat cttgtagaga aataacatag cggttgtgct
     7261 tttgctgaat atcaaatggc ggttcgatca aatgaaaaat acccgcatct atcagtgctg
     7321 ctaattcttt cgtccgaatg attggcggac cgatcgttaa aaagctattt agatgagtaa
     7381 accatcccca tagtatttct ttgtattcct tagcagaaaa aacgtgccat tcaatagcct
     7441 gccggattgg atcacgccag tcttttaatg catcaaatgt tgaagcaaat gctcctgtgc
     7501 aattgccttt ttccgcttct tttatctgcc cttcaataaa tattcggcta gcttctgaaa
     7561 agggctcatt ctgataggca agaggagttt ctaaatacga ccaatcccat ctataaggga
     7621 ttagtgctgg atgctttccg agacactcct ctgaagagag ggagagaaaa tcatgctcaa
     7681 aatttttctt tggaatcggc aagtgatatt cttcgatgat tttcttgtaa taaaacaact
     7741 caacgtcttt tttcagatag tcaaaaaatg tttcgccagt caaaaatccc gactgatgcc
     7801 attgctccag gttttctttt gttagtaacc gtggccatgc cattgctccg ccttgctttt
     7861 gatttcttcc tcgagggtaa taagggagcc cccttcttga tccactgtat acaatcggtt
     7921 ccttgcctga tggatgatag accagttttc ccttttcgtt ctcgaagcgc cctcctcttg
     7981 atactgtaaa caatccgaca taatcaaaaa aacttaagcc tagacctctt aaaaagacat
     8041 tttctttagg cggtatcttc gaaagatcta catctgcagg attagaagga agctgataca
     8101 gtaaaacatt tttctttgcg tagtctaccc acactctttc ttcttctgtc tcatcatttt
     8161 cccagtgacc gcttgctaaa acaagctgat cgatagaata gctttgctga tctgtctgta
     8221 aaagataacc atcttttctt ttgcttactt tttttactaa agaataaaca aactgaatag
     8281 aagctggaaa ttcttcttgc aactttgaga aaaaccagcg ttgatagagt ccgtataaac
     8341 atcgagtaga ttgctgatct ttttccagac gattgatttc ttctataatt agttgcttat
     8401 ttttggttac cgtctgttcg atataatctg tagcaggtcc tttactccat tgaaacaagt
     8461 tgggtccctg tgttaccaat cctccactgg acaatgtttc atctgtaaaa agcgtcactt
     8521 gttgagaaac agaattcatt aataactccg gtggctgatt caaccgccaa actcgacctc
     8581 caggaccatt gggatcgaaa agaaaaagtt caagagaatg tctgtcctga ttattatgga
     8641 gaatcctttc tgctacactt aatcctctag gtccggcacc aatgatccct attttcattt
     8701 gcatgcctcc atttcgataa attttttcaa taaaacagca tggttctgat gttcattttt
     8761 tgcaccataa acaaaagtaa tgactggatg ttctttcata agttgactga gtttaaaaat
     8821 agcttgcttc ttttcttctt cgaaaatcaa ttcttgctgg tatttctttg caaatgaagc
     8881 aaatcgttct ggtacatggt caaaagtttt gcgtgcttca ttactaggtg caatttcttt
     8941 caaccacaga tctaactgcg ctttttcttt tgaaattcct cgtggccaga atctatccac
     9001 gagtattcta tagccatcct ctttttcagc atcaaggtaa acacgtttta atttgatcat
     9061 agttgtttcc ttctttctac cttttcgctt agcttatata tgtagataat agcacaaaat
     9121 aaaaaaaatg aacggcacaa ctatccattg cgccgttcat gtgatcttgg tcaaaatcta
     9181 atgattgatt cggtttttaa agctgtcata caatcatctg ttttatactt ttttactttt
     9241 gatttttcca gaccatccgt catatccacc cttaagtaaa taaatctctt gataacctgc
     9301 tttacgtaat ttatttgctg tacggatgct caaacttttc ttttgatcgt aaatatagac
     9361 aggctggtct tttcgaatag atcctaatga atctttcaaa acagtgtaag ggatgtttct
     9421 tgcacctaaa atatgcccag catcaaacac atctttttcg cgaacatcga ttacttgtgc
     9481 ttttctcatt ccttctcgaa attcttcttc tgtcaaagtc gttgctgaac gtttggccat
     9541 tactcgtaga tagacttccc aaaggatgat tgcaagcaca atcaatagca aaatgatatt
     9601 aattacccat aaaaccatgt aatgcctccg tttttttaag caaatttcaa ggctaatgag
     9661 gcagcaccaa tgacaccagc ttcattacca agctctgcta atttgatttt tgttgattct
     9721 cttacttgcg ggaaagtaaa ttctttaaaa tatttctcta cacggctacg taaaaattct
     9781 cctgctgccg atactcctcc acctaacacg atacttgacg gatttaatgt attccccaag
     9841 tttccgcaag ctaaacctaa gtagaagcat acgcgatcaa caaccatcaa tgcaaacgga
     9901 tcgtcttctt gagccatttc aaagatatct ttacttgtta cttcttctcc gttgtctagc
     9961 attgctttca gttttgaatc ccctgcgtat tcttctgcca agaaacgacc taggcgtacc
    10021 acacctgtag cgctagatac agtttctaaa catccgcgtt taccgcaagt acattcgaac
    10081 ccttcaggat ccacagtgat atgtccgatt tctcctccag ctccgctcac gccgtgaatc
    10141 aaatttccgc cagcaacgat acctccgcca acacctgttc ctagagtaac gaatacaacg
    10201 tctggatcgt tttctccagc acctttccat cgttcaccta aagcagcaac attcgcgtca
    10261 ttatcgatag cgaatttgat tcctgttcct gcctcgatca gttttttcac aaattgaact
    10321 tctgtccagt ttaagttata cgcaccaata accgttccag cttcgctgtc cacgcttcct
    10381 ggggtaccca tgccgattcc gataaagtct tcggctttca tattatataa attcaaacga
    10441 tgattgattg attcaatgat ttcaggaacg atatgcttac catcatccaa aatattggta
    10501 tcgatgctcc atttttgctg aatctctcct tctggtgtta aaattgcaaa ttttgctgtt
    10561 gttccaccta aatcaatccc aataatcttc ttgtccatta caaatttcct ttctttcggc
    10621 tttcttcaat tcggtgctct ctttttaata tgcgccacgc tgtcatgtat gtatcatgat
    10681 caatcaaacg gctatcatac agattacgca attcgatagt cattaattca atgtcataga
    10741 tgcgtgcacc cacatagata aagatgccaa attttttgaa cagctgctgt acatcatata
    10801 agctctccat ctttaagcac ttccttcata ttatacaagt aaaccatatt tttttaaacc
    10861 cagtaaaata catattacaa aaagaaatac aaaaactagt gtagaaagaa tcctttggtg
    10921 tatcgaatag cttgtctgtt gtttcggtag acccaaaaca ttccctaaca ctaatccgcc
    10981 aattagccca ccgacatgtc cccaaacatc tacagaggta tccagaagac caaaaacaaa
    11041 tgtcaacaca ataaataaaa gaaaatgtct gactagctgc tggatcgctg ggttatgttt
    11101 aaaatgaaac cctaagataa acaacgcacc gaacaaaccg aatatcgaag tacttgcacc
    11161 accagacaga acattcgctt cgttaaaagc aaagcttgct gtatttccca ttatcccgct
    11221 gaacatataa atcaagaaaa aacgccaatg gccatagatt gcctcgattt gctgccccat
    11281 aaaatacagt atcagtgagt tcattacgaa atgcatcagt ccaaaatgga tgaagattgg
    11341 tgtcactaaa cgccaccatt cattgaagtg aacaagataa ggaccataca ttccggcata
    11401 ataagggatc tcaagtcgcg gaaataattc catcatgagg aagaccgctg tctgtatccc
    11461 taaaaaagca taggtccata acggtcgtct taaccatgca cgcaatttta tttgctgttg
    11521 ataattcatg aaaacctccc atttcatttt agtcaataaa caattgttgg atcttctgat
    11581 caaagctctc cggttgccat tgttcattca attgttcacg aaaaacaagg ctacatgttt
    11641 ttttatgaaa attatttaaa taccggtcgt aataaccacc gccaaaaccg attcgataac
    11701 cggctctatt gtagataata cctggtacaa tgaggagatc gatcatctcc ggcaaataga
    11761 gcttctctga ttgtggctca agaacaccaa aactcgaacg tacaagaacg gactgttcat
    11821 ccatttcata aaacgccatc tgacgtttcg gcaaagtttt aggtacaact attttctttt
    11881 gttctttttc tgctcgttgg ataaccggct gtgtatccag ttcaaattca ttcgataaag
    11941 tcgtccctat cacttttgct gacttccagt actcgctggc gaatagctgt ttatagattc
    12001 gctgttcttt ttcctttttc ttgccacttt tggccagatt tttcagcttt tcgattcctt
    12061 tccggcgtaa tcttactttc aattggtcca aaaactcacc cttccttctc tcataaaatg
    12121 gtataggaaa aaaagaaaaa tagcaatcga actttcaaat aaatgcataa atagaataaa
    12181 aagaagacct agaaatgatt ggccttcctt ttttaatgaa tttcaactgt tcagcgcgtg
    12241 tttttgtctg ataaaaagtg catacagaaa atatggcgca ccaaaaaggg caatcaaaat
    12301 tcctgtaggt acttccccac ttggtagaat cactctagca accatatctg cactaagcag
    12361 aaaaatggaa ccgaacaaac cgctcagaac aaaggtccag ttattttctt tacgtacgac
    12421 tagcttcgca gcatgaggag ctagtagtcc tacaaaagat aaactacctg tcaccgctac
    12481 actaccgctt gccaaagcaa cagctaacaa caaaaggatt ttttgacttt tagccacgtt
    12541 aaggcctaat ccttgcgcgc gttcttgacc aaatgatagc aatacaatcc tctgtgcata
    12601 aagtcctcct agcggaatta agataaccgc ccatggaagc aatgcccaaa cataaggcca
    12661 gctcgcaccc cagattgatc ccgctaacca tgcattcaca aatccataat tttccttaga
    12721 taattggatc gtgcatagca atgttaatgc agaaaggccg gcattaacgg caataccgga
    12781 caataagatt cgattcatac ccaaaaagcc atttcgatca taggccgcaa gatagacaca
    12841 gcaagcagct aaaacaccac ctagaaaggc tacaactagc aatagaaacg gcagcgaata
    12901 gtgcgaaaag aaacctaaat aaagcatcac taaaaatcct gctccggcat taatgcccaa
    12961 tatactagaa tctgccaatt catttcttgt tactccttgc aagatataac cagaaacgcc
    13021 aagacaagct cctgctaata aactgatcag taatcgagga actcgaaatt ctctaacaat
    13081 cagcgccaaa gaagatgatt ctttccctat gaacgtggca aaaagctgat ccaggtaaat
    13141 aaaaggcgta ccaatcatta aactacctat agacaggatg acaagtacaa aaagcaagaa
    13201 atagaacgtc caatatattt ttttcaccgt tgttccctcc ttacttgtat cagtaaaaac
    13261 ggaacgccaa tcaatgcaat gactaatcca aaaggtgttt caaaaggggg attgatcatc
    13321 ctcgctgtaa aatccgccaa cataaccaat ccaccgccaa ctaaaaaaga gacaggtaga
    13381 acataacgat agtccgttcc aaccattccg cgaacaactg gcggaaccaa cagcccaata
    13441 aatgaaacag gtccgactaa cgaaacggca atggctgcta acactgcaac tagacagaaa
    13501 acaatcaacc gcgttcgttt aggatgactt ccaagagcta atgcatgtgt ttcactcaaa
    13561 tgaatcaaag atattgcttt tcctaatgaa aaagaaacca aaacagcaag tatatataaa
    13621 ggaaacaata ctgctaattg tttccatgtg atattggcag ctccgcctac aaaccaaaaa
    13681 gcaagatcct gctgcagatt aaagaattgg gtaagtgctt ggctgatagc agtaaaaaaa
    13741 gaactaatag ctacgccagc taaaatcagc tgtaaaggat tcatgcccat tttcgaaaac
    13801 tgcaaaaaac caaatacgag caaagttaca gcaaaagaac ctgccaatga cattagaaac
    13861 acagaaaaat cagaagcttt aggccaaaaa gcaaaactaa gggccattgc caacgaagca
    13921 cccgcgttga ttcctagtaa gcctgaatct gctaaaggat ttcttgtcac tccttgcatc
    13981 aatgcaccgc tcaatgcaaa acaactccct acaagaaatg ctcccgccac acgaggcaaa
    14041 cgaatatttc gtatgatttg gtgactttgt tttgatctat caaaatcaaa caacgcctgc
    14101 caaaaatctg ctttttccac agtcgctgcg ccatttgaaa gagcaacaaa aaaaataatc
    14161 aaaagaacaa aacagaccaa aagcaggagt ctatttttct tcatagtcct cataattgat
    14221 cctcctgctc ttttggcaac aaatcatagg ttagaaaaac tggtttattt gttcttggaa
    14281 gcgtgacaat cgttgcatca atctcaaata tttttttcat acactctttt gtgatcactt
    14341 cttcaggtgt accttctgtg atcaacttac cgtttttcat tgctaataaa taatctgaaa
    14401 aacgtgaagc atgattaatg tcatgaatcg acatcaagat cgttgtttgt ttcatttgat
    14461 tgatttcagt caataactgt aggatctcta attggtgtgc cggatctaaa aatgttgtcg
    14521 gttcatccaa gatcaagata tctgtttcct gagcaagcgc catagcgatc cagacacgct
    14581 gggtttgtcc accagacagc gttgcgatat cccgcatacg aaatccagct agacctgtag
    14641 catccaacgc ccactcaatt gcttcatgat cttgcgtgtc catgccactg aacagttttt
    14701 gataagggta tcgtccgtaa gatactactt cttctactgt catacctgta gggtgttcac
    14761 tagtttgtgc taataatgca attttttttg caagttcttt tgaactgtat gtagagatat
    14821 cagagttatt gacaatgatc tttcctgatt tcacaggtag tatacgggta aaacttttca
    14881 ataaagtaga ttttccactt ccatttggcc caatcaaact agtgattttc ccttcaggaa
    14941 tcgtaacaga catgttttca atgatttttt tgttttcgta gccggctgca atgtcagctg
    15001 ctcttaatct cgtcatttca cccatccttt cgctgaaaat cattctcaat tatgacgctg
    15061 tttcaacatt tcgtcaagcg ctactttcat atctatagcc aaatgaagcg gttgacagcg
    15121 ttcgaaatat caggaataat aaccaatgag aaagattctc aattatgtta ggaggaaaga
    15181 aatgaaatta aaaacaaaag tattcacatt atctagcata ttgctgctgg ctggagtact
    15241 gagcgcttgc tccccctctt cagataaaga aacagctaca actgccaatt cgacgacttc
    15301 atccaatagt actcatacag tcacagatac acttggacat aaagtagaga ttccaaacgc
    15361 gcccaaacgg atcatcggca gttacttaga agactatctg attgcactag gggaaaaacc
    15421 tatcgcccag tggactgttg gcagtggttc cattcaagat tatctgcaag atgatttaaa
    15481 ggatgtccca acgatttctt atgaccttcc ttatgagaaa gtgttaagct ttgagcctga
    15541 tttgctgttg atttcttctt ccgctactgt cgaaggcggg aaatacgaac aatatagcaa
    15601 aatcgcgcca acttatgttg taaaaaacgg aacagatgtc acatgggaag aacaactaaa
    15661 agatgtcggc aaagctttgg caaaagaaaa agaggcagaa aaaatcattt ccgattatca
    15721 aaaatccatc aagaaaactc gagaagaact agcagataaa atcgagaaca agacagctgc
    15781 agtgttgtgg gtgaccaaca actcagcctt tatggtcagc aaaaatcggt caagtggaag
    15841 aatcgtatac gacgatctgg aatttggtgt tcctgatcta gtggaagagg tctccaaaaa
    15901 agccacttct gactggtctg ctgtttcctc tgaaaagcta gctgaattag atgctgacta
    15961 catcatcctt gtgaacagtg acaaagatgc tgccatgttc aatgaaccaa gctggaagaa
    16021 tctaaaagca gtgacagaca atcatgtact gcaattcgga cctgaatcta gttggctata
    16081 taacggacct attgccagtc aggaaatggt caatgatatc aaagaaaaac taaattgatt
    16141 ttaactaaaa aaagcatctt cacgattagt cgtgagatgc tttttttctt attaacaaac
    16201 tagacgcata gattttattc ttcttttgaa gcaaccctat tttttactct ttcccatttt
    16261 tgaaaacgat gggcatatag attttcttga tccgttatcc cctcatactc ctctataagg
    16321 gcgaagtctg aaaaatcaat attcccaata tacgtatcgc cttcaaacgt gctatcgatc
    16381 aatgttcgcc agatccgacc agtgtaagga agaagttctt gaaaaatccg actaccaccc
    16441 gaaacataga tatcttctga gatcgtttga ctatacgaca aaagttcatc gatcgaatgc
    16501 attacttccg cattttcttt cgtgcgaaaa tttggttgcg ttgtcaacac gatgatatgg
    16561 cgataaggaa gtgaaagatc ccccattcct tcataggtct ttcttcccat cactaaaatt
    16621 cgatccattg tatgttcgcg aaagaaacgc atatcatttg gcaatcgcca tggtaacaaa
    16681 ccatttttgc cgatcagacc atttttatct tgtgcccaca tggaaataaa catattatcc
    16741 gctccttatg attttagatg ttttgagata aaaaaagtct caattctttg accagcaagg
    16801 aattgagaac tttaaaaaca ttattttgtt tcacggtgca aagtaacttt gcgttcgcgt
    16861 gggcaatatt tttgtttttc caaacgatca ggattgttac gtttgttttt gcttgttaga
    16921 tagttacgtt ctttacaaga agtacactct aaagtgatgt ttacgcgcat gtttttccct
    16981 cccattttta caagaatcct ctaagatgac tctcagactt gattatctta ccatgatttt
    17041 ttctagattg ctagtgtttt tcacgatttt gttaagtaaa actacttgac gataaataca
    17101 acggttttac ttctcaaaca aaatcttttt ctttttgtat aaataccctt acacttgttt
    17161 atatagatta tgttttttaa taaaggggga tactaatatg ccatggaaca tgaaggactt
    17221 tcctgcatca atgaaaaact tagataaatt gacaagaaaa aaagcaatcg acatcgcaaa
    17281 tgcattatta gatgaagggt accctgataa ccgtgcgatc ccaattgcca ttgatcaagc
    17341 aaaaaaatgg tatgaagatg ctagtgaatc agaaaaacga actttcgaaa aagaaaaaaa
    17401 tccaagcaag acggatgaac acgatacgaa tcctcgtgct ggaaaactgc ttgattcaga
    17461 tgtgatcgta gaatatgagg aagaacaatg gatcgtgaaa tctaaagggg caaaaaaggc
    17521 aagcaaccat tttgatacaa aaaaagaagc aatcgaaaaa ggaaagcaag tagcacaaaa
    17581 caaagaatca actttgttca tttataaaaa agatgggaca aaagaaaaag aaatcgatta
    17641 ttgaacatat gtaaaggggt tgtgacgaaa atcgtgtcac aaccccctta tgttcgaaac
    17701 aatggtgttc ctctgccaac tccgaaccat aagtcataat atctataaaa cgggaagagc
    17761 tgttttttga aattgtttct tatcactcag tgttaaacgg taggagaaaa cctctttttt
    17821 attgatattt gattttcatc aaacagtcac atagtcgatt gcttctgctt gcggaatcgt
    17881 ttcatctaca aagtagactt tataccatgt caagaaactg aagattgtcc agatttttct
    17941 ttgctcttct ttacgtcctt cgtgatggtc atctagcaac ttcatgattt tttcttgatc
    18001 aaaaaattct tttgcaaaat ctgcttcgaa taatgctttg acttgttcgt agccgtgttc
    18061 ttctttcaac caagctttga ttggcacagg gaatcctaat ttcacacgat tcgaccattc
    18121 ttccggaaga tgtttgttcg cagcttgtct gaaaatatct tttgtgttgt tttgattcaa
    18181 taaaaactta cttggtattt gttgagccaa tttcatgact tcgatatcca ataaaggtac
    18241 tcggacttcc aaagaacttg ccatagaaag tttatctgcc tttaacaaga tatcttttgg
    18301 catccattga tgaagatcga catattgcat cttattgact tcatctttga ttccttctgt
    18361 tttctcgtaa tgaacagtca tgatttcatc cacagatggt gctgtttgat attttggtgt
    18421 taacacttct gatgcttctg attcttcgaa cactcgagca tgaccgataa aatattcttt
    18481 tgcaggagca agtgattcat ataaatggat ccgtccatgg aagtttttca tttttccgat
    18541 tttacgtcct aaattatagc gtgttccttt tggtaatttt ttcaatcctt gagcaatcac
    18601 acggatgaat tttgaattcg tatggaatcc atacgtttgg tatcccgcaa acagttcatc
    18661 cgccccttct ccagattgga ctacccgcac actttgtgaa gcaagatttg ctaaaaagta
    18721 caaaggtacg caagaaggat ttgaatcagg ttcatctaag tgatactgga tcaaagggaa
    18781 tgctttgaac gacatgtctc cgtcgatcac tgctgctgta ttatctaagt ccaatagttc
    18841 ggttagtttt ctggcttcaa tcgcttcatt ataggtttta tcatcaaacc cgattgagaa
    18901 agaatgatct ggtttcaata cagaggttac ataactggaa tcaaccccgc tggataagaa
    18961 agaaccaact tctacatcac tgaccgtatg tgcttcaata gaagcttgaa cggtttcgtc
    19021 gattttttcg atccattctt gtcgggaatg cgtttctttt gtttcaaaat ctgcatccca
    19081 atattgctga atgtctattt ttccatcttt atacgtataa taatggcctt caggcaaacg
    19141 atagacatct ttgaagaatg tttcgccgtt taatggagaa tattggaaag tcatataagg
    19201 tttcaacgct tctttgttca attctttgtt aaaatcagga tgaggcagaa aacttttgat
    19261 ttctgaacca aacataaatg ttccgttcat ttctgcataa taatatggtt tgatcccaaa
    19321 gtggtctctt gcaccgaata actcattctt ttcattatcc caaatagcaa aagcaaacat
    19381 tccgcggatc ttttgcaata attctgttcc ccattcttcg taaccatgca aaaggacttc
    19441 tgtatctgca tgtgtttgaa aaacgtggcc agctgcaatc aattcttctc tcaaaggctg
    19501 gtaattatag atttcgccat taaaaataat gatctttgtc cgatcttcat tgtaaatagg
    19561 ctgagtaccg ccctctaagt caataatact taatcttctg aatcctaaag caacatgttt
    19621 atcgatatat tctcctgaac tattaggtcc acggtgaacg attcgatcca tcattgtatt
    19681 aataattgtt tttttgttgt ccttgtcgtt aacaaaacct acaataccgc acatttaact
    19741 ttcacatcct aatataaaat tactgtttat ttcattcata tgcatcttat catagaataa
    19801 atagcggacg aatattttct ctcttacaat ttcgtcacgt gaaaacaaaa gatgaaagtt
    19861 tctgttttta cagaaaataa agtcccaata tctaatttac catgttttct tttttgaggc
    19921 taccttattc tcttcgtgtt aaaaaaatat tagtggatgc tatttccaaa aaaaccagca
    19981 aaaaaagaca cgacctaaag atcgt
//
LOCUS       DBNICI010000037        19681 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.037, whole genome shotgun sequence.
ACCESSION   DBNICI010000037 DBNICI010000000
VERSION     DBNICI010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.037"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            398..628
                     /locus_tag="AC1ZCC_001097"
     CDS             398..628
                     /locus_tag="AC1ZCC_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629364.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            787..1620
                     /gene="terS"
                     /locus_tag="AC1ZCC_001098"
     CDS             787..1620
                     /gene="terS"
                     /locus_tag="AC1ZCC_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HIQ1629365.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            1613..3028
                     /locus_tag="AC1ZCC_001099"
     CDS             1613..3028
                     /locus_tag="AC1ZCC_001099"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HIQ1629366.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            3040..4569
                     /locus_tag="AC1ZCC_001100"
     CDS             3040..4569
                     /locus_tag="AC1ZCC_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HIQ1629367.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            4487..5539
                     /locus_tag="AC1ZCC_001101"
     CDS             4487..5539
                     /locus_tag="AC1ZCC_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HIQ1629368.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            5541..5858
                     /locus_tag="AC1ZCC_001102"
     CDS             5541..5858
                     /locus_tag="AC1ZCC_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629369.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            5985..6608
                     /locus_tag="AC1ZCC_001103"
     CDS             5985..6608
                     /locus_tag="AC1ZCC_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HIQ1629370.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            6623..7513
                     /locus_tag="AC1ZCC_001104"
     CDS             6623..7513
                     /locus_tag="AC1ZCC_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SU10 major capsid protein"
                     /protein_id="HIQ1629371.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            7536..7751
                     /locus_tag="AC1ZCC_001105"
     CDS             7536..7751
                     /locus_tag="AC1ZCC_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629372.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            7763..8104
                     /locus_tag="AC1ZCC_001106"
     CDS             7763..8104
                     /locus_tag="AC1ZCC_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629373.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            8104..8475
                     /locus_tag="AC1ZCC_001107"
     CDS             8104..8475
                     /locus_tag="AC1ZCC_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629374.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            8468..8863
                     /locus_tag="AC1ZCC_001108"
     CDS             8468..8863
                     /locus_tag="AC1ZCC_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HIQ1629375.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            8865..9242
                     /locus_tag="AC1ZCC_001109"
     CDS             8865..9242
                     /locus_tag="AC1ZCC_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator family protein"
                     /protein_id="HIQ1629376.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEIPQYIEWSER"
     gene            9243..9851
                     /locus_tag="AC1ZCC_001110"
     CDS             9243..9851
                     /locus_tag="AC1ZCC_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HIQ1629377.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            9851..10192
                     /gene="gpG"
                     /locus_tag="AC1ZCC_001111"
     CDS             9851..10192
                     /gene="gpG"
                     /locus_tag="AC1ZCC_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone G"
                     /protein_id="HIQ1629378.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            10434..13223
                     /locus_tag="AC1ZCC_001112"
     CDS             10434..13223
                     /locus_tag="AC1ZCC_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HIQ1629379.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            13213..13953
                     /locus_tag="AC1ZCC_001113"
     CDS             13213..13953
                     /locus_tag="AC1ZCC_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HIQ1629380.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            13950..16712
                     /locus_tag="AC1ZCC_001114"
     CDS             13950..16712
                     /locus_tag="AC1ZCC_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HIQ1629381.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYE
                     RPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYS
                     GYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIIN
                     WARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISS
                     IVIPPK"
     gene            16725..17633
                     /locus_tag="AC1ZCC_001115"
     CDS             16725..17633
                     /locus_tag="AC1ZCC_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HIQ1629382.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            17633..18253
                     /locus_tag="AC1ZCC_001116"
     CDS             17633..18253
                     /locus_tag="AC1ZCC_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629383.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            18257..18706
                     /locus_tag="AC1ZCC_001117"
     CDS             18257..18706
                     /locus_tag="AC1ZCC_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629384.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKAKLDGLANITFEKVGTV"
     gene            18907..19200
                     /locus_tag="AC1ZCC_001118"
                     /pseudo
     CDS             18907..19200
                     /locus_tag="AC1ZCC_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19214..19645
                     /locus_tag="AC1ZCC_001119"
     CDS             19214..19645
                     /locus_tag="AC1ZCC_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629385.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
BASE COUNT         7132 a         2707 c         4076 g         5766 t
ORIGIN      
        1 ttaatttatt tcagcggacc actcgctgat aaataaaatt atacacttag tatttatttt
       61 cacaatatta atttgtcact gtggcggaaa gggtagacgc taagcatgtg tgttaggtca
      121 atgcttcggc aaccatgcaa ggttcgattc cttgccagtg actttggcaa ccgaggcatc
      181 ggcggtttaa aaatataggg gtgcgcaatt tcgtacgcgt tttgtgcatc gtgtaggttg
      241 ctattacata actggttagg ttagattgag atttgggatt cggtacaaat gaatcgtcaa
      301 atgtctcaag cacaggatcg gaaacgtccc tgcctgtgca ttacatatta gatcactctt
      361 tgagtggtct ttttctttac ataaaggagg ctacataatg agaaactact ggtatgtatc
      421 gctaactaat gaatatcctc gaaccattga tgattgttca gtgcgtgttg tgcgttctgt
      481 acaaatcaaa gggaagtact ctattgtcga aatggggaga gaagcagaac catgtgagat
      541 tgatgcgtgc aagctcgttt attgcggtca tggtttctat gatgaaccaa acattcaaaa
      601 taatattaac aagaatttga gggattagaa tgcaagaaat ggcttaaaaa tctccaatca
      661 ggacaaatca agttagacaa agtttccgat ttaaagatat tgattgaagc tgatttaatg
      721 ctaaaagata ttgaaaatta gaaaacaaaa ctcaacctaa acagaatgtg aggtggtgtg
      781 tattgaatgg caagacaacg tgatccaaga cgtgatgaag ccaaaagaat ttggttagaa
      841 tccaacggag aaaagcagtt aaaggaaatt gcatctgaat taaatgtttc agattctcag
      901 gttagaaaat ggaaatcgca agacaaatgg agcgctgaat tgattagtaa cgttaccaat
      961 ggcaaaagta acgttactaa tcaaggtggc gctcctattg gtaatcaaaa tgctaaaggt
     1021 aacaaaggaa atagccgagc ttctccgcca gtgggtaata aaaacgcttt gaaaacaggt
     1081 gagtatgaaa caatattttt tgacacgtta accgataatg agaaggacat ctattctagt
     1141 ttgaatgatg atccttcttt tgctttgtcc gaagaaatac gacttcttaa aataagacaa
     1201 cttcgaatga tgaaaagaat ccaacaagct gaagctggac taaatgatga agaagtcgaa
     1261 cgattgcagc agctaagaaa gattaaaact ccgattgaaa aaaatggtaa aaagctagaa
     1321 atcaagcgtg aggttatgca agatgtgcag attagcagaa aaaaacatcg caaaattgat
     1381 gatattcttt caattgaaga ttcattgact cggattagca accagttagc taaagccatc
     1441 aagcaaatga atgaacttta tatgaatgaa tacagaactg atttaattaa agctcagact
     1501 gataagatcc aagctgagac aaatgaaatt ggcggaaata attcaggtga agaaatagaa
     1561 gaatggaaac aggcagtttt aaatgccgca aacaaacggg cggtgaaaga aaatgaataa
     1621 tgaatttatt ccttttgctg atattggttc tgccattgat tattactatg ataaaccagt
     1681 agctttttgc caagatattt tgcatttgaa tcctgatgaa tggcaagaaa atgttttaaa
     1741 tgatttagct gaattttcaa aggtttctgt tcgttctggt caaggagttg gaaaaacagc
     1801 attagaagca ggagcaatac tttggttctt aacgtgtcga ccctacgcta aagtaatagc
     1861 aacagctccg acaatgaagc aactttacga tgtactttgg gcagaggtag ctaaatggtt
     1921 aaatgatagc ttgatcaaaa acttactgaa gtggacaaag accaaaattt atatggttgg
     1981 tgattcagag cgttggtttg ctacggctag aacagcgact aaaccagaaa gtatgcaagg
     2041 ttttcacgag gaccatatgt tgattgtggt agatgaagct tctggtgtgt ctgatccaat
     2101 tatggaagct attcttggta cgctatcagg ttttgataat aagctgttga tgtgtggaaa
     2161 ccccaataat attgaaggtg tcttttatga ttcccataat tcagaccgtg ataaatacag
     2221 agttcataaa gtatcaagct atgatagtaa acgtacaaac aaagacaata tagaaatgat
     2281 tcttaaaaaa tatggaaaag aaagtgatgt tgctcgtgtc cgtatttttg gagaatttcc
     2341 gaaaggtgcg ttggattcat ttatcagtct tgaaacagtt gaattggcta cagaaaaaca
     2401 aattagtgat tctttagtca ataaaacaaa ggttgctcat attggtgtcg acgtagctcg
     2461 atatggtgat gattctacga ttctctttcc tagaattgct accagggcat tggagtatga
     2521 gaagtattca aaacgtagca ccatggaaac aacaggatat gtcatcaaca tggccaagaa
     2581 tctaatgagt caatatccga gtattgataa agtgatgatt aaagtcgatg acactggtgt
     2641 cggaggtggt gtaaccgacc gcctagaaga acttatagaa gacaaacatt atccttttga
     2701 ggtgtttgga gtgaataacg gttcaacatc agaagacgat ttttacgata atttaggtac
     2761 tcaactatgg ggaaacatca aggaaatgtt agaagaaaat atgacagcaa atcttaacgg
     2821 agaacagcct gttattgaat tgccttctga tagttcgtta atcaaagaat taagtactcg
     2881 caaattcaaa atgacaagta gaagtcgtat acgtttagaa agtaaagatg atatgaaaaa
     2941 gcgaaatatt ggtagtcccg atattgctga cgcactggct ttagcgtttt atgagccacc
     3001 aagtcactat caatttattc aattttagga ggtgagcttt tgattagtac agtattatct
     3061 ttagagagat ataagaagct acgtgttaaa tatgcaacac aaattgaaga tggaatgttt
     3121 gatccgaatg gttttataga agatatgaaa ccattttttg ctgatcgcga aagaaaatat
     3181 ctagcttata ctagcgaaaa gaatgaaata gataatagac caaagcctaa cactgatatt
     3241 gtaaaagtta ataataaact tcatgctggt atgtattcaa ttgtcgtaga ccaagcagtc
     3301 aatcatttta ctggtatacc tatcaaatgg gattacgatg tatcggaaca aaaaagaaca
     3361 ctcattcaaa gattaaaaga taaattctta aaaaatgata tagaacttcc gacagttcca
     3421 gaagctttta acaaattaac aagcaatctt gattccatga gattcgcaat gcttgattct
     3481 gaaactgcaa cttttcaagg agcttgtgga gtagctttcc gattgctaga gcctgtggaa
     3541 gaagatgatg gttggaagtt aagagcaagt aatattgaac cttggagagc agaaagatat
     3601 ggaaatgccg gaatctatat caaagaaaag tatgactctt accagaaaaa attttttcaa
     3661 gaaatgaaag ttatgacaag aaataaaatt ctgacatatg cttgctatgg tgatttgaat
     3721 tttgttacaa gcggaacgtt caaaaaaatt gatgagaccg ataacccttt ggggacgatt
     3781 accttggcag aatttaaaaa taatacgaat cgttattgtg attttgaagt agctgaggaa
     3841 attggagatg caattgatcg tgctttgtct gatcaacaaa acgaaatcga acaatttaag
     3901 cttgcgtaca tgcttgttac tggcacaaca atgagcaaag gcactgctaa agaaatgatg
     3961 aatcagctag ggattatcaa cttaaaagcc cctactgcaa aagctgaata tgtcacaaaa
     4021 aatttagcaa aagatttcaa tgaatatcat atggatctat tgaaaaaaca gttttacacc
     4081 atttgtaaag caatcgattt taacgatgag gtgtttaaat caaacagttc tggagaagct
     4141 cgcaagtggc aaatcattag cctagaagcg aaaacaaata ctaaagagca atattttaga
     4201 gaaggattga aagaatgcgc agaaacaatt gctgcttttc ttaagtttca tgacaaagta
     4261 gaaattgagc ctgaaaaaat tatttttact ttctctagat ctttgccaac tgatttaagt
     4321 tatttagctg aagctttacc taaacttgca ccatacgtat ccaaacgaac tattcagagc
     4381 caaattccat ttgtaacaga tgttgattat gaaaatgaaa tgatggaatt ggaaagtgga
     4441 agtgcttatc cagatagtga atacaatttt ggcggaggtg gcaatagtga cgatagaaac
     4501 gaagtattgg accaaacgtc gagaactgga ggatcaagca aggctcaaac aagaaaatca
     4561 gacacttaaa aaattaacta gtgtatttcc tgaagcactg aaagagatac aagcaaaact
     4621 attatcacaa gctgacttac acaatatcac ttatccagaa atgatggagt tttacagtac
     4681 aagtaatcag aaaaaatatc gtgaatatgt ggaaaaaaat tataagtcat taaaaatgta
     4741 tgatgcaaaa tacaaagagt ttatcgatga attttttcca ccatttgact atgcaaaagt
     4801 caatcgctta ttacaaatac gatcagatgt atttaaaatt cttgcagaat atgcgatgga
     4861 tgcagatgtg aatcaatatt tttctgatcg cttagaggaa attcttcaaa gaacatattc
     4921 ttctaatgct aatgtttttg ttcaactttt aaacgttgat ataccaaatt atttaccaga
     4981 aaatgaaatt aaacattatt tgaactatcc atggtcagga aagacatttt caagaagact
     5041 ttggggaaat atttcatcgc ttgaacagaa actctctaat gctattgtaa aaagtgttgc
     5101 tagtggagaa ggcgttatac acgcattaaa caccatgaga ttagactcag aaatttgtga
     5161 catgtttaag ttagaagaat caaagtataa caaagcgata gaaaatctcg ttcgaacgga
     5221 gtatgcaaaa tttgcacaag atggtattga aaaatcatat ttagaaacag gtattgagga
     5281 atacaacgta ttgactgcaa aagatgagag agtttgccgg atttgtggag gaaaggcaag
     5341 taagaatccc tataaactga aagatgctgt cataggtgaa aatcgagcac ctttccatag
     5401 tcgttgtaga tgtacggatg ttcctaattt accaaaatta ggaaaggata ttgatgaaga
     5461 atatgaccgt ttatttggcg atttattaga tgagtttgca catgattctt ttggaattaa
     5521 tttgaaacgg aggaagtaga atgaaagatt tttttgaagc agtactaaca attaatgtaa
     5581 atgctgatat tgcagaagcc tacaaaacag ctattgagtc tgagaaccat cctaatggct
     5641 tgagagacca ttggaatggc aattatgcct acgtggttat tggcgatcaa actgttaatt
     5701 atcaagataa tactccagtt gataagaata ccgttaattt aacgattcaa ttattatctc
     5761 attcattacc aaatttaaaa gaaacagttg attggtatga aaagatggga tgtattgttg
     5821 ttagaactga ctacaaagaa ggaaagtcta gtaattaggc ttttttattt tgtccgaaat
     5881 gacgttaaac tagcgcaata ctgggcttgg ttgaatggtg gggcgcaact attaaaactc
     5941 aaagcaatgc ggggcgtgaa aacgaatcgt ggggcgaaag gagaatgagc atgaaacaca
     6001 aatcattaat gccaatgaat ttgcaatact ttgccgaagg tgacgatcaa aagttttctt
     6061 ttgatgactt caaatctttt gtggaatcca atgaagaagc tcaaaaattt attcagtcac
     6121 aatctcaaag tgttgccgac aaacaattgg aagcttggaa acaaaataac ttagataaaa
     6181 ttaaacagga taccatcaag gaatatgagg aatctaagaa aaacaagtca cctgagcaaa
     6241 ttcaactgga aaaactacaa gctgaatttg aagcagaaaa agcgttgcgt gtgacaagtg
     6301 ataataaagc atttgttgca gaacaaattg ctggattaga actagatgga gagttaaaag
     6361 agtctatttc tcaatttatg ctaaataatc ttgttagttc ggatacagat ttcactaaga
     6421 atgctgttga aggttttaca agtgttttga atgcaattaa tgagaaacat gcagatgcat
     6481 taaaagaact acaaatgaag tctgcatttg gtggaactca acaatcgaat aaccaggttc
     6541 agcagaacaa tgaaacatta acaaatccag aagaacaatt aggacaaatt cttcaacaat
     6601 ttaactagga gagtgaaaaa ttatgaaaaa aacatcttta aataatttag agtatttgga
     6661 tatttcacca gcgattaatg ctatgcaagt accaaataca ccttttttaa gctatttact
     6721 tggtgctgga aaaacagagc cagcaaactc gacagaaatt aaatggcgtg aatatgatat
     6781 caacaacgat gattcttctg aaaaacttga gggcggagaa tatccagatg ctgaatcagg
     6841 tcgaacttgg tttaacaact atactgaaat ttttagaaaa tcaacctctg tatctggtac
     6901 attagatgct attaatgtga atggagtcgg aaatgaatta actaatcaag tagcactacg
     6961 tggtatggaa atgaaaattg atttgaaccg aaaattgatt actggtgtaa aagctgatga
     7021 aaatggttct aaaggtcgtc gaatgaatgg gattttgaac ttgatcaatt cagcaaataa
     7081 ggcagaaaca gccactgcgg gtgcagtaac aagaaaagat atcgatactt tatttaaatt
     7141 gatgtatgaa aaaggttata tgggagaaaa actatgcttg atttctccag atatgcagga
     7201 gttaatgact gatgagttag atggaaaatc aacaaaaatt gttcagttcg gggaaagagt
     7261 aacttttgga ttgcaaattg gaaatatcgt gtctaattac ggtacaggta ttgctctact
     7321 agaaccatca ttgccaaaag gaacaattgc cgcaatcgat actaattatg tgaaactacg
     7381 tccattacgt gaatggagag cagaagaact tgcaaaaaca acggattcta gacgtatcgg
     7441 tcttgtaggt gaatactctc ttgaatacaa tgcttcaaac tctggggcaa ttttaaattt
     7501 aaagtctgaa taaaagggag ttagtactcc cttttttgat aggaggaatt acagtggtaa
     7561 aaaaagatga aactaaaaaa gatgaagtcg tgaaatatag agtaggtaaa actaaaaatt
     7621 ttgttgggtt tgttcatcct aaaactcgta gatttatcac agcagattca aataacgaat
     7681 ttatcatttc tatagatgat aaagaagcaa ttgcaatttt ggaagatgca attgatgtta
     7741 atgaaattta ggaagtgatc tgatggatga atcgctaaaa acggaaatca ttgagtctac
     7801 aaaagaagat tttccagatt tgagtgaaga acgcataact aatttattag aaataatttt
     7861 gctagaaatt gaatcataca acacttgtaa aaatgatatt tcatgggaaa agttaaaaag
     7921 cgtgattagt gaagtgttgt ataaaataat aaaaaatgaa tcagaaaaaa cagtatcttc
     7981 agttagacgt ggcgatacaa cgattagtta tgcttcaaca acaaatgatg ttagtgagtt
     8041 acttctaggc tatggtgatt tgatacgaag agttattggt tgtggaggat tggagttttt
     8101 ttaatgaatg aagcagatat tttagaaact acttatgaag atagttgtat tattgaaaga
     8161 ctgacggaca ttgaagattc taatacaaat attactattc aagattacaa aaaagtatat
     8221 gataatccta tttcttgcgc tctttcacaa ggtcaaatcg atggactagc agtcatagaa
     8281 gatggagaga tggtaaatgt ttcaactgac acatataaat tatttgttca tcctaagatt
     8341 aaactaaaaa aaggagatcg aataacaata actcaaaagg ccagtggctt aattttttct
     8401 ctatttgcta ctaagccttt ttactatcct agtcattgtg aagtgaattt gataggaagt
     8461 gagaaaaatg ggtaatctca aatatgaatc aaatgcagaa aagatcattg agaattttaa
     8521 aaatatgact gtaattgctc aaaaagaagg aatatccttc gttaacgatt caatgaataa
     8581 agtcgttagt ttagctaaac cattaactcc tgtaaaatca ggtaatttaa ggcgaggata
     8641 tcgtgtagta aaagctagaa aactatcaag cggtcgaatt gttggagaag ttataaacaa
     8701 tgaacattat tttaaatatg tagaagaagg tcataggact aaaaatggtg gctttgtcaa
     8761 agggaaattc atgttaactc gtgcaacgaa tcttgcaaat atgtcttata ttcctcgaag
     8821 atttaaacaa atggcaataa aaatcgttaa gaaaggaaag taacatgaaa gatgaaatca
     8881 ttgctgcaat cagtagcaaa ttaaaagaaa tctatcctga tgggacaata tatcttgatt
     8941 cagttatgca gtcaactaaa gatttttatt tcgttttatc agtaatggaa tctggaactg
     9001 aaaatgtagg aattgatatt caaaatgttt ctttcttaat tgatattgca ttgattgata
     9061 ataaccctaa tagaaattta attaatgaat tagtctcacg ttgtgggact ttttttaata
     9121 cgattacaat cgatgagcaa atattatttc caaaagccta tttacctgat gaagcagatg
     9181 gtgttcaaca tattcgtttt acattagaaa ttccacaata tattgaatgg agtgaaagat
     9241 aaatgggaga aaaaagaagt aaaaccggaa ttatttctgt cgaaaaacct acttggtttc
     9301 ctttggaaga tgagacaggg aattttccgg tatatggtac tgcgttgcca atgggaacag
     9361 cagtaagtat caaaccaaca gctaattatg aaacaacaca agattatggg gattcagtgg
     9421 ttcaagatca atttacagct tttggtggtg cagaagttga actggaagca aatggatata
     9481 ctcataaagt tttatctaca attactggag ggaaaattgt taaaggtggt gccttgcgtt
     9541 ctggagaaga tattgcgcaa gatggtgcgt ttgcatatag acgtaaaaaa tcgaatggga
     9601 aataccgtta tacggtcttt tataaaggac agtttgctct agattcagat gaaacatcaa
     9661 cgattgaagg aagtaaagta agttttactc atccaacttg gaagggttca tttgtagatg
     9721 taccaggact tggatatatg tattcagttg atgaagatga tgaaggcgtg gataaagcaa
     9781 tgatcggaaa ttggtttaca aaagtagcaa ttccaattga agaagcagaa ctttcaggag
     9841 gtacagaata atgtcaaaat atcaaacaac gattaaatta acgaaaaaag acgaagaagg
     9901 taaatatgag caagtacaat ttaaatctgc tgaattttta ccaggaacag ttgtagaaga
     9961 tgctgcagga gttatggaag aaatgcaaac agcgactgat aagcaatcag ttaaaaaagc
    10021 tttaagtcgt gcttattcat ttattgcaga tactttattt gagggacaat ttactggtga
    10081 agattattgc aaaggaattg atgctcgtga gattgcctca ttgacaggaa aattattgaa
    10141 gtctgttact gcaggttttg atgaaactta tacagagacg aaaaaaaagt aagtgaggcg
    10201 ctcaagtcac cttcatttaa gtattcgatt acttaccgag aattagatat aaagacgcaa
    10261 ttactcgaag caggttggac gttaccagaa attgaacgta ctgacttgga tgagttaatg
    10321 cgtctttttg cttttagaga tgcggtaaaa gagcatgaag atgttgagta ctatgataat
    10381 ttcactcaat tttaggaggt gatactttga ataacgatga cctaattctg aagatgatac
    10441 tagatgaatc tggttttact gccggtatga ataatgctgt caaaaagttg aactcttttg
    10501 atgaaacaat cgaaagaaaa agtagaaata gtggcagttc attaggtagt atttggaaaa
    10561 tatttgctgg aagttttcta gctagcggag taactagaat cgtaggagct ggctttgatc
    10621 taatcaaggg ttccataagt ggagcggttg atcgagtaga tacgatgaat aacgctctac
    10681 gaaatttcca aaatatggga tttagtaatt cagaaattat gaagaatatc ggaaagaatg
    10741 ggcttttatc tcaaggtatt caaggacttc ctactgcttt gaatgatgcg ataagtcatg
    10801 ttcaacttct tgcttcttct acaggtgatt taactcgttc aactcaaata tttaaagctt
    10861 taaatgatgg aattcttggt ttcggtggtt caactgatca agttaatgaa gctgttattc
    10921 aattatctca aagtttctca aacggaaaag tagacgcaca aacttggaac tcaatgatca
    10981 acgctcaact tggacctact ctttctgcta ttgctaaaaa gatgggaatt acaatgggag
    11041 atctgaaaga aggtttatct caaggtaaga tttctgttga agaattccaa aatcaattaa
    11101 tagaaatgga taccaaaggt ggcggaggac ttaaatcatt aagtcaaatc gctaaagatt
    11161 ctactaaggg aattaaaacc tctatacaaa atgcaaaaac agctgttaca cgtggtgttg
    11221 gtgaggtaat agaaggatta aataaagcat tagtagattc agatttaggt ggatttaagg
    11281 gaattattga taaagtgggc agttcaatgg aatcgttctt aaaagtaatt gctgcaaata
    11341 ttcctacagc agtatcattt ttaagtaatt tatttaacga aattcaaaaa tttggttctg
    11401 ctttgaaatt catgatgccc tttcttgttc ctgcagctac cgcttttgga gcgttcatgt
    11461 ttcaacttaa aggtatacca gcaataataa aaagcttcaa taattttaag aatgccataa
    11521 ttggcgttgg aaattcatta aagattatgg gggcaatagc tgctgctaat ccatttgttt
    11581 taattattgg tgcagtcgtt ggtgcaatag ctgttttcgg ttactttatg gcaaccaatg
    11641 aagagcttag aaacaaggtt atatctattt ggaatgatgt gaaagattcc gtaattggtg
    11701 tattaaaaaa tataaaggac tggggaattg atacttggaa ttctgctaaa gaaatggcat
    11761 caaatgcagt tgagggtgtc aaagatgctt ggtcaggaac aaaggaatgg ttttctaaca
    11821 cctggaaagg tattaaggat ggggccacag gtttatttga taaaacagta gagacttcaa
    11881 gaaatgcagt cgatagtgta aaaaatgcat ggtctaatat gaagaaatgg ttttctgata
    11941 cttggcaaag tataaaagat tcagccagag aaaaatggaa tggaatcaaa ggttctatta
    12001 tgtcagttgc tggtccattg attacaggta taaagaatgc atttttacat gttacttttt
    12061 atctagagac cttatggaat aatttagttg agattggtaa aaacgtcttt gagattttaa
    12121 aaaatgttat acttgcgccg gttttgttta ttacctccat gatttctggt ggatgggaag
    12181 aaacaaaaaa taatatgatt gctgtttgga ataatattaa agaaagtgcc ataaatattt
    12241 gggaatctat aaagaatata tttgtaagtt attttacaaa tatttatttt gctgcactca
    12301 atatttggac agggttcaag cttacattga taaatatttg gaatgaagtg gtaaatcaag
    12361 ctaaatcgat ttggttaaac ataaagtatt tttttattaa tctttggatt gatataaaat
    12421 attttgcaat tcagaaatgg attgaattga agttttctat tattcaaact tggatcgatt
    12481 taaaatataa tgccattact acttggaata atattaaaca gtttttcaaa gatacttgga
    12541 aaaatattaa agatacggca tacaatacgt ggatttctat aaaaaatacc atgattaata
    12601 catggaataa cattaaggac tctttctgga atattgttac tggaattgtt aattctgctg
    12661 aaaatgcatg gacaaatcta aaaaatggtg tttcaaatgc tattaatcgg gtaaaagaaa
    12721 tctttaattc gttaagggaa gtcaatttat ttgaaattgg caagaacatc attgatggac
    12781 ttataaatgg tgtaaaagaa aagtggaatg ctttgaaaga tactatcaaa ggtattgcag
    12841 gtaatatcaa aaattggatt aaaggagctt tagatattca ttctccatca agatggatgc
    12901 gtgatatggt tggtaaaaat attgttcaag gtattatcgt tggtattgat aaagagcaaa
    12961 gtaagttaga tcaaaccatg acaaatttag taaaaacacc gtctgttcaa cctgtagtaa
    13021 atggaataag tcacctacca attacacaaa caaagcaaaa tatatctgct agtgaaacaa
    13081 aagaaattca tttacattta aatgtttatg gagatttacc tgattcaatg gttcggaaga
    13141 ttaccacaaa aattaaaaca gaattgacaa gacaaatgaa acgagatgct ggtgcagtag
    13201 gaggaacatt atatgcaact taagagagga caatttttta ttaatcaaca ttattcttct
    13261 gaatttaatg tgtatattca aaacaggcct gcttctgttt cagctagtcg agtaattgaa
    13321 ttgagagagc gagaaggaaa tgattctatc attattgata aaggctatta caaaaatgtg
    13381 actagaaaga ttgagtgtta ctataaagca ccttcaattg atatggtgca ggagtgggaa
    13441 gatcgaatca ctgaatggtt agatatgtcg tcttatagtg attttattct ttactatgat
    13501 gaacaatata tttatcaagc tgtagtaata gaaggacctg aatttaaagg aacaagaaaa
    13561 acaggaaata ttgtcccttt tgaatttaca gtgagtattc gaccatttaa ggaaaattac
    13621 aatggtagat ttactattcg acaaacagaa acttttgaaa tttataaccc agagaagtat
    13681 tcttcaaaac cgcttattaa attgagtggt tctggagatg cttcttttta tattaataag
    13741 gataaatatg atttgaaatc gttggacaga gaattattta tagattctaa attagaagag
    13801 gcttatcgaa aattagatgg taatttagaa catcaggatc aggtcacctt atttttagat
    13861 tttccatttt tatatcctgg aaaaaatgaa attaaatgga cgaaaaatat tcattcattt
    13921 gaaataatgc ctaggtggtg gagaaaagta tgaaaccaag aatatatagt cctactgaaa
    13981 cagatttctc aacgaatggc ttaggaattt taaaggattg tacaagatgt ggaatatatg
    14041 aagtagctaa tggaaaatat gaattagagt tggattatcc tttaggaact agatttgatg
    14101 aatattttga aaatgactat caaataaaag caaagccaaa cgatcaagaa gagtatcata
    14161 tcttttttat tgatgataaa gatatagata cttttttaaa tacagtaact atttatgctc
    14221 agagtcgtac aaatcgactt ggaagacggg tagtcactct tgtggaagtg gactctaaaa
    14281 ctggtcaaga agcgatatca attattgaaa ctaaaatgga taaaaaatct gacatacgac
    14341 tttattctga tattacggct gtttctagca caatctttga agcgagaaat gttttaaatt
    14401 gtattgctgg tgaacaagga tcattgcttc aatattgggg cggagaaatt aaacgtgaac
    14461 catttaagct ttctttgtta aagcgaagag gtcgcaataa tgttggaacg attcggtatg
    14521 gaaaagatat gtctggtttg aaggtcaaat tagattggac aggtataaaa acaagaatta
    14581 ttccatatgc tgatcctcaa agtgaagtag gtacgactaa tcgaatttat ggttcgccag
    14641 tagatagtgc ctatattaat aactatcctg atgtctatac agaacatgtt cagtttacag
    14701 aagaacaagg agtaaaagac attaagagct taaataaaat agctaaaaat tactttaaaa
    14761 cgatcaatcc tggctgtgat aagcctaaag tttctattac ggttgaattt gataagttga
    14821 ctgatagtga agaagcgaaa gaatttgcga agattagaaa ttatggtctg tttgatacat
    14881 ttaaaatata tcataaaaaa tataatattt atcttgaatc aaaggttagt ggactacaat
    14941 acgattcttt atctgaaaaa actttgaaat tagaagcagg agacattcaa gttgcttttt
    15001 atcagcaaca agctgtaact attcaggata aattaaaaga ttatgcaacg aataattata
    15061 tgagtgattt taatgattat gtttcttcaa tgattacagg tcaaggcagt gcaggaggat
    15121 atgtagtttt atggccaaaa gaaaaacctt ccaacatctt tattatggat aatccagatt
    15181 tagaaaaggc aagagaagta ctaagaatga ataaaaatgg gattgctttt tctaagaaag
    15241 gatggcgggg gccttttaat tcagcttgga cattagatag tatatttaat gccaatttta
    15301 tacaaactgg attaatcaaa gcagatatct ttcaaaattc ttttaacaag actggagatg
    15361 tattgaaatt agttaatgga ctactccaaa tttggaacaa caaaaagaaa ataatggagt
    15421 taactaaaaa aggaatggaa ttttggaatt ctaagggttc aattggaacg attggaacaa
    15481 ctgattctgt tggtaatcct tttcctgggg cttctactcc aacccctatt gaagataatt
    15541 ctttagttat tcgtacaaat ggagacggca aatatatttt gatttctcct aaagtaggaa
    15601 aaggattagt tttattagga aatggtaaag caatttattt tggagactta gatgtacaag
    15661 gaaaacttac agttaaagga aaagaaataa ttcctggaca aggtggtggt ccatcgggag
    15721 gtggagaatc tactggtggg tatcctagtg aattaaaaac agatgcagaa aagagagctt
    15781 ggagaattta cgatattttg tgtaataacg gatttacgaa acaatctgca tgtggaatat
    15841 tggggaacat tcaacaagaa acaggaggaa cttttgatcc tgatactgtt caaataggtg
    15901 gaccagcata cggattagtt cagtgggatg gttcttcata tcctcttgtt ggtccagcaa
    15961 catgggatgg aaaagtttat gttcaaaact tatttaatgc tgcaggtatt aaagaaccaa
    16021 taacgagctt agatgcacaa gttcgattgc ttatttggac atttacaaat ggacaatgga
    16081 tgggcgtagt acaacctacg acggttgatg gatttaaggc ctgtactgat ccaagacaag
    16141 cagcatatgc ttttgaacga aactatgaac gtccggcagc gacacatcct gaacgtcaag
    16201 attatgcagt taactggtat aacaaattta aagatttaaa accaggagga gctactggag
    16261 aagcaggact aaaacattta gagtctttga ttggacaacg aattggcaat ggtcagtgtt
    16321 atggcttgtc tgcagaatat tcaggatatc tgggtggttg tggcatgggt gctggaacaa
    16381 aatatggttt aactcatgtg ataggaaata cttctgcagc atctgatatt ggtattgcct
    16441 atgattggtc tgctgttggt tggaaagtga ttcaaaaccc tagatatgat caattagtag
    16501 ttggtgcaat tattaattgg gcaagaggtg gacaagtggg ttcatggttt gcagatggaa
    16561 cttatggaca tactggcgtt atccgaggct tagctaatgg tcgtatgcaa acttatgaac
    16621 aaaacacaga gttaggtatg atttgtggaa agttagatcg ccagtattat agtgcaagtg
    16681 caatttcttc cattgtcata ccaccaaaat agggaggtga tttaatggca aagtggaatg
    16741 tcatattaag tacaacagaa ccctataatt acgtggggat gattcaagtt cgacaaggca
    16801 ataagaatac agaggttatg gaagcgacta tagttgaaaa tggtcttccc tacgatttat
    16861 cagaatgtaa ggtatatttt gaatcagttg taggtgggaa atatccagtc caattagaaa
    16921 caaaaattgt ggatgctaaa aaagggaaaa ttaactatat ttttgataaa tattccatgc
    16981 agtgtttaca tcgacaaaca gccaatttca ttatatttaa aggagaagac ttgattggaa
    17041 caactcaaga cttctcttat tttgtcatta atgctgtttc aaaaacagaa ggagaaatgg
    17101 gttcttattg gcaatcaatc gaagatttaa ttgcggatat gacggacttt attaatgaaa
    17161 ataaaggcga ttttacggac tggatgaatg aaaggaaaga agaatttgaa cgttggagac
    17221 aagaacagga acacagtttc caagattgga gagaaggaca agaatcagac tatttggcat
    17281 ggtttgaatc aatcaaggat attttaaaga cgattgatcc aggtggagtc atgttagccg
    17341 aactaacgga tgcgcgagta gatattcaag gagttcgtca tgcgtctatt tcagagcgtt
    17401 tgctggcaga tatggactat ttatatcaga aattgcgagc aacgcttttc acgattgaat
    17461 acggggagat tgaagtgact gatattttgc aggatgatct cttttcagat aatcatgaag
    17521 tcgaaaaagt tggaactgta gcattcccga ttgaagaagg agccttgatc atcgcaaccg
    17581 ttgatgatcc aaagcaaaat gtgttcactc ttgagaaagt tggggtgatc taatggctaa
    17641 aactaaacga atgatggaaa cggatgaaaa aacgggtgta caacgccaat tctttccaat
    17701 cacacatgct tccgcagttc ttgggttaga agaaataata gcaggagaag caacagtttt
    17761 atctgttaat ggaaaaattg gagccgtcgt cattactaaa gaagatttgg gattagagaa
    17821 tgttctcaca gaattaccct atgcaagtga agaagatgac ggcattatca cggcagaaat
    17881 gtatcaaaaa attttaaaca gtggagaagg tgactacgtg ttgccagttg ccactatcga
    17941 ccgtttgggt ggcataaaag ttggtgaatt attgacgatt gatgagacag ggaaagtctc
    18001 tgcagtcaga caatctgatg tcaatttttc gctggagtta aaagaaaaac tcgattcact
    18061 gaaaaattat actgctggag aaaacatcac tattgatgaa gatggaacaa ttagttctac
    18121 aggtggtagt gggactggtg gtgtcaatca gtcatatgta gatcaaaaat tccaagaagc
    18181 tgtaaatcaa gcagaaaatt acacaaacga aagaattcca aattttactt ttgaaaaaat
    18241 tggggaggta tagagaatga cagatatcgt aaaaataaaa caaagcaatg tacaggttta
    18301 tccacaaact cattggaatg ctatagaagg caaaccaaca acggtgaagg gagataaagg
    18361 cgatcctggc caagcagcaa caattaccat aggaaccgtt tctagtggtt caaccgcttc
    18421 tgttacgaat gtaggaacat catctgcagc tagatttaat tttgttttac caaagggaga
    18481 taaaggtgat cctggaataa atgccacaac tacagctgta gctacaacta cagcaaatgg
    18541 gttgatgtcg tccacagata aaactaagtt ggatggaatt gcagctggag cacagaaaaa
    18601 tccaggtaat gctacaacta cgacagcagg tttaatgtca gcaactgaca aagctaaact
    18661 cgatggatta gccaatatta catttgagaa agtggggacg gtttaatggc tgatattgtg
    18721 caattaaaag aagacggagt tgctaaatac ttaaaaacgc atgtagaagc tattgatggt
    18781 aaagaagcat tggtgcagac agatggagac caagccattg caggacataa gaatttttca
    18841 ggttctgtaa caatcaataa taaacgtgtt ttgacgactg atgataacag atatgaagtg
    18901 gtaaatctgg ttgtcactaa cggtaataca gggacagcaa agctttatcg tgaaggaaaa
    18961 acagtttcta tatattttgt ggctttaaac ggaaaaagta gtggcgggaa tgattcggtt
    19021 attttaactg ttccagaagg ctatcggaca ccaattagtt ttgaacaact ggttgggtca
    19081 atagaccgtt ctactttgaa cagtgctcaa ctatctattg gcgcagacgg agccattaaa
    19141 tggagaagaa attcaagtta tggatcagct tattcatttg ttatcactta ttcaatttaa
    19201 gggaggaaat ctaatgaaag tagtttacaa atcaatcaag ccttacggat tcgagcaaat
    19261 cattttgaac aatcaagaaa atatccctga aaactgtaca gagattaaac caccagttcc
    19321 taactggaga ccaagatttg attttgataa aaaacagtgg gttgagttag ctactgaaga
    19381 agagaaaaaa gggaatgctg ttgatagcgt agatgaattg gctaatatta aggcattata
    19441 cgaaacgctg aaggcagaaa atgatgaatt gaaacagcta aattctaaag caatgctaaa
    19501 caatgtagca atcaagcaag aaaatgtctt attgaaagaa aagtcagaaa gtttagcgca
    19561 gttgaattca aaaacgatgc ttgcttctgt acaaaatacc aaggaaatcg aagaaatcaa
    19621 aaaacaatta caaggtggga agtaacatgt attcatatga agatatcaaa ctaatgtatg
    19681 a
//
LOCUS       DBNICI010000038        19603 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.038, whole genome shotgun sequence.
ACCESSION   DBNICI010000038 DBNICI010000000
VERSION     DBNICI010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.038"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            289..501
                     /locus_tag="AC1ZCC_001120"
     CDS             289..501
                     /locus_tag="AC1ZCC_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629386.1"
                     /translation="MNENQLRELFKTNEANKTMEATFYETQKSLALIAKQAKYFYDQL
                     ILQGFNDAQAMEFMMRTFSASNQQKE"
     gene            508..732
                     /locus_tag="AC1ZCC_001121"
     CDS             508..732
                     /locus_tag="AC1ZCC_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629387.1"
                     /translation="MRNYWYVSLSNKYPQPNADDPIRVVQSVQIKKKYSIIEMTREAT
                     PKEIDKYNLRYCGHGYFSEQNIQTNIERYC"
     gene            759..1292
                     /locus_tag="AC1ZCC_001122"
     CDS             759..1292
                     /locus_tag="AC1ZCC_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HIQ1629388.1"
                     /translation="MVNLTPKQKAFADEYIKNGGNATQAAIKAGYSKRSARVIGKENL
                     TKPNIIQYINERLNPIEKKRKLSAEDALNELIDIWQGEVQISVSKQIDRLEKNKVIKH
                     MQYEYTPDLESKLKALDLYLKYKSLLSQTQLEKAQTEIKLMQAKLEQLQINSERSTEE
                     KLDELLEKISGELDGTS"
     gene            1279..2523
                     /locus_tag="AC1ZCC_001123"
     CDS             1279..2523
                     /locus_tag="AC1ZCC_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HIQ1629389.1"
                     /translation="MALVDIYNPKQIDVLNKTIKNDWFITLLHGAKRSGKTKINNDLF
                     LFELRRVRKIADEEDIKEPMYILAGVSSATIQKNILQELYNMYSIEPKFDKHGNFKLF
                     GVKVVQAYTGNIGGVGAIRGMTAYGAYINEASLARQEVFAEIVSRCSGTGARILADTN
                     PDNPEHWLKKEYIDNSSKNIQSFHFELDDNTFLSERYRNNIKESTPSGMFYDRDIKGL
                     WVSAEGVVYRDFDASKHYIQSKDLPPLSNFYCGVDWGYEHWGSIVVIGETDDGTAYLI
                     EEHATQFEEIDYWVDVAKGIQERYGLRVPFYCDSARPEHVARFVREKINAKNAHKARL
                     SGVEEVAKRFKQDKLFICQDRVMKFRDEIYQYIWDKKKGEPIKEYDDVLDSVRYAIYT
                     HELLKKPKVNVNEKIKRIKRMF"
     gene            2536..4035
                     /locus_tag="AC1ZCC_001124"
     CDS             2536..4035
                     /locus_tag="AC1ZCC_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046798 - viral portal complex [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0051027 - DNA transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HIQ1629390.1"
                     /translation="MDKVNEFEYGADIHYSNDVNTNYVKFSVDSNLHYRFSSAEDLLN
                     DLDTLAAMIKHHHEYQVKRLSVLDDYYKARNTNIMDNRRRREKEKADHRSAHNFGKVL
                     CTFDVGYNTGNPIKVQIEDTNQQKEIEEFNTNNDIDGLNAELWLDMDKYGRAYEIIYR
                     DSDDTDYVDLANVFETFVVYDTTVKREPILAVRYPKTRFNKDADKQYIQPIVYTKEKS
                     ITYDETTLTAIELKNPQDEPHEYKEVPITEYSPNRFRMGLYEDVLSLIDLYDAGQSDT
                     ANYMTDLNDALLVISGDIEAAGLSTEDAIKQKEANMLLLESGTDVNGNKTSVTAGYIY
                     KQYDVNGVEAYKYRVRKDIHEISMVPDLTDDNFSGVQSGEAMKYKLFGFEQMTATKQR
                     LFKKGLMRRYRLLFSLKSSISEMDNSDLKGLRVIFTPNLPKAILEELKSLVDAGAELS
                     QETILGLASFVPDVQAELKRVNKETQKQIGIFDSDGEEVINNKKDETGG"
     gene            4040..4966
                     /locus_tag="AC1ZCC_001125"
     CDS             4040..4966
                     /locus_tag="AC1ZCC_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HIQ1629391.1"
                     /translation="MNSQEYWIKREKEWQKQQIKDDKKRMAEIKSRMQYAQDAIQKEI
                     DAQWDSFSNGQKITRSEAMKRASEMDVKAFARKAKKYVKEKDFSPTANQELKLYNLTM
                     RVNRLELLKANIGLELISLFNELDKYFSNELTKAGLAELKRQAGILEMTIASSGYAKL
                     IELVINSSFLSDDVSFSDRLWMYQSELKSELDRLLVRSITMGKNPKQLASKLAEYLTA
                     EGRENTKFNTQRLMVTETTRVQVGIQERSYRDAGITQYIYIAEPTACKLCIPLNNQVF
                     DVADMQPGSNAPNMHPFCRCSTAPYIERISSR"
     gene            5093..5695
                     /locus_tag="AC1ZCC_001126"
     CDS             5093..5695
                     /locus_tag="AC1ZCC_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HIQ1629392.1"
                     /translation="MKKRLFMPMNLQFFSEPGDGGSGDEGQQGNLPAGSQETPPEAKE
                     ENNTGKTFSRDEVAKMIAAETNKAKAAWEKELAAKKEEAKKLAKMNAEEKLQHELEQK
                     EAEIAELKRGQALSEMTKEASKMLTDANLPHDDDLLGLIVSDDADATKQAVAVITNFA
                     SLIKRENARQTPPNEGGQFTASKNTKETVAKLAAKNRIIK"
     gene            5708..6631
                     /locus_tag="AC1ZCC_001127"
     CDS             5708..6631
                     /locus_tag="AC1ZCC_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HIQ1629393.1"
                     /translation="MKKKQLLPMNLQMFAQTWDPDNVLVYETKEGKIPDKYNTLILSE
                     VMENSKIMQLAKYEEMTDKEKKFEYFAEGPGAYWVGEGEKIKTSKPKWMQATMTAKKL
                     GVILPVSREYLNYKLSDFFEEMQPKIAEAFYKKFDAAALLNKENPFPQSLDGSVISAG
                     NVVEGGLTYDNILALEDKLAENEFEPNAFISNRKNRTELRSAAQTVGSNVEFIYDRST
                     NTIDGLPVVDLKSLDKGTLYAGDFNYMFYGIPYNISFKISEEAQLSTLTNEDGTPVNL
                     FEQELIALRATMDVGFMIVKDEAFGKISPKA"
     gene            6649..6912
                     /locus_tag="AC1ZCC_001128"
     CDS             6649..6912
                     /locus_tag="AC1ZCC_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629394.1"
                     /translation="MPNQKTWTGKVGDTKTFTISAVPADASDAAAVVAATTATSSDGA
                     IATVTKNENGGFDGTIAAEGSATFTFTSGEFTTSINVTGQPAS"
     gene            6924..7253
                     /locus_tag="AC1ZCC_001129"
     CDS             6924..7253
                     /locus_tag="AC1ZCC_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HIQ1629395.1"
                     /translation="MMIADDIKKLLKGTIDEKLEVIERRTNERMKTLLNTQEVPKEFE
                     TVVYEVSLKRFNRIGQEGMQSYSQEGLSMAFPDSDFSEYQNEIDEFKRKDQEELYKPK
                     RGRFKFV"
     gene            7250..7576
                     /locus_tag="AC1ZCC_001130"
     CDS             7250..7576
                     /locus_tag="AC1ZCC_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629396.1"
                     /translation="MRFTDEIIFVKRSSDSKYDPDLGEWVEGKPERTRTEANVTDIGT
                     DRSVTIFGSVEEGAKVIRTQPLFSIPTFDYIEIEGKTWQQKTARNPAYRNSLIVQEVV
                     LDEGTT"
     gene            7560..7898
                     /locus_tag="AC1ZCC_001131"
     CDS             7560..7898
                     /locus_tag="AC1ZCC_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HIQ1629397.1"
                     /translation="MKAQLEYKGIDQLMRHLKKAATLNDVQKVVKSNTAEMTERMQKG
                     APVDTGYLRRSINMNLLEAGLTGIVGPTADYAPYVEYGTRFMSAQPYVRPAFNYQKVK
                     FMAEMKALVK"
     gene            7898..8290
                     /locus_tag="AC1ZCC_001132"
     CDS             7898..8290
                     /locus_tag="AC1ZCC_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HIQ1629398.1"
                     /translation="MIKTRDQSIFDELFKISQNKLGYKTYDYKTLEDVGYPFVEFENT
                     QTIHEVNKTDIKGSVIVVLSVWGLQKKRKQLSDMASALFNEARLIEATEGYYWALNYQ
                     ASGIQVMDDTTTNTPLKRAVVTLEFRIR"
     gene            8301..8813
                     /locus_tag="AC1ZCC_001133"
     CDS             8301..8813
                     /locus_tag="AC1ZCC_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HIQ1629399.1"
                     /translation="MEALKGIDVILLYRLLKKETQEAAWKMAFQTEHENGLSRDSDST
                     VTKDGNVQSLSPVEYDFSATSIVAKGDSHVDEMKQALLNGDIIEIWEINKAEQGTDDN
                     ANKYKATYYQAYVSEFTPSAAAEDNVELSLSFAVNGVGQDGYATLTEDQADVVQYAFK
                     DTVKATSTGA"
     gene            8859..9227
                     /locus_tag="AC1ZCC_001134"
     CDS             8859..9227
                     /locus_tag="AC1ZCC_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HIQ1629400.1"
                     /translation="MKLKIKGKEYSFKFGTKFVRELDKVMPFVDGNMEFGMGLSAKVL
                     PELRSYNVNTLSRVLEIANRTEDESITLDELDDYIDEVKDIEKLFDEVLKELAESNAG
                     KLAVRNLNQKLKEAEKQQVE"
     gene            9281..9568
                     /locus_tag="AC1ZCC_001135"
     CDS             9281..9568
                     /locus_tag="AC1ZCC_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629401.1"
                     /translation="MTNISDIERMTLYEYNIRMTAAQLSWLDKEKLIHELAWANQQVQ
                     AEKKVGKKTVPVYRSFEEFFNYQKIEDSIMGVSELSKQDKKFQSLLTKANS"
     gene            9585..12704
                     /locus_tag="AC1ZCC_001136"
     CDS             9585..12704
                     /locus_tag="AC1ZCC_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HIQ1629402.1"
                     /translation="MEQFSVEALLKATDSGFVKTFKDAQDAVKTFEKNSNSMTTAVGK
                     VMQGTGAAMTKYITTPLIGVGVAAAKVGGDFEAQMSRVKAISGATGDTFEQMKQQAID
                     LGAKTAFSAKESAAGMENLASAGFSAQEIMKAMPGLLDLAAVSGGDVALASENTATAL
                     RGFGLEASEAGHVADVFARAAADTNAEVGDMGEALKYVAPVANSMGISLEETAAAIGI
                     MSDAGIKGSQAGTTLRGALSRLARPTKAMQDTMDNLGVSFYDADGKMKPLKTQVELLK
                     KAFEGLTPEQQQNALVTLYGQESLSGMMALIDKGPDSLGKLTKSLKDSDGAADDMART
                     MQDNMNSSIEQMFGAFESAAIVIQKILAPSIKKVADAISGLVEKFVSAPESTQKLVVA
                     IGAIVAAIGPLIFMIGSVIIWINRVKVAFKALSESSKLFSGLSKAMGLLTNPVFLVIA
                     AVALLVVGFIYLWNTSEDFRNFWIGLWEGIKSAVSSAVEWIQNAWKSTGEWFNNLWKS
                     IKEGADNVWTTVQEAPGKAADWIKNKWTGTKEFFSNLWSSIANSASEIWNSLKEGVIS
                     VIDDLVSSAGEKWEGFKNTISTAWKTITSKIKSGFDFILKYIGPFVSSFSDVFSNIVK
                     AITSIFAEVKNIIVNAWEIIKSLIAAPLLFIIDLITGDFEQMKEDLDLIWNTLVQSVV
                     NIWTSVKNIFTEYIGAIVNSAVSLWTGFIQSISNIWNEVVYQATMIWIDLKLFFTNLW
                     IDIKYSAIQMWINLKFSIIQTWIDTKYGAIELWNNLKQWFFQTVNNIVQTLIKSWNSL
                     KQGTIDLFNNTVQGAKDIWTSFKSWIGDLITGTKDNIIQGWENLKQGTIDTFNNLVNG
                     AQEAWDNLVNAVSDTVDRVTGWFDNLKNIDLLAAGKAIMDSFLEGLQNAWKSVQDFVG
                     GIGDWIREHKGPIQYDRKLLIPAGQAIMNGLNKGLTGGFNDVQNTVGSMADFIAELFN
                     ANSDVDIAANLKNANKNIGAQVEHKINMGGSTKPAVFKINLGRQSFRLFMDDISQAMG
                     EGADINLEF"
     gene            12716..13504
                     /locus_tag="AC1ZCC_001137"
     CDS             12716..13504
                     /locus_tag="AC1ZCC_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="distal tail protein Dit"
                     /protein_id="HIQ1629403.1"
                     /translation="MDQRENKMYSFKDTTINLTSSKRFLPTSAMMYDGMYLEDLIDGY
                     QTLTVEGREMLSVEVEQQEIQIGSIITNQKIPSRTLKITYKLEDRDPEKLQFKFKELL
                     NYLYRNEDVEIRFHDELDYYYYGRYTSTDTVPGDSNSIISSFNVFCADPLKYTKECVS
                     DGYIGNPIQFPITPRKIEVTLSMNNSIKITNGEQNITITDAAIKTGDVLVFDFSDEQV
                     TVNGEDCTSMIDLESDFENFYLKQGQKITSNNGKLKIFYRGATI"
     gene            13501..16332
                     /locus_tag="AC1ZCC_001138"
     CDS             13501..16332
                     /locus_tag="AC1ZCC_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HIQ1629404.1"
                     /translation="MSETVYFFDHLQKLIKRKNTRSLIEVSQEKEISSDKSDLMKDTL
                     YVTTKYDKEIEDARYMAIRENESSFSLYRITKVSDPFETLEFTGLGFATNELDAYIIK
                     DIRPSGQPLKNVLDRLIEFTEGNWRVGHVEAMLPTVTATFYYVSVKEALKELQTLGME
                     FVFRCSLNSDGIKDKWIEVYEQIGEESNTRFVYGSKALTVVREIDRSSISTSMIGRGR
                     GEEVGDGYGRRIEFTDVEWKKSNGDPLDKPKGQNWLEDPESTQKYGIPQKDGSMRKRE
                     TVVVFDDIDDPTDLLKNTYSTLIDSARPLVQFKAEVTGGDVIGNTVTIHRYDKGYHYK
                     TRIYKTTFNRLTGQTNIELGDNLTQDVRKQTASIVNNINSLESSKMTFYESTEIGKYQ
                     DDIMRGAGDNGGSIYWVNGIEAGVSDSREIYETVYMDGPNIPRSRFFMVQNNSGISFK
                     QCKKGEWQTIQDVHNGDSTTAWTLDGTFNANFIRAGILSGILVQGVALKTFDDKDFQL
                     VAEGGELSFEKQVKSTGLDDVHGERLGSIVSTYGSKGINGFAVWKEPNYIFSINAGDG
                     GDRGNPIFQIPADVTADKRKYNLYGDGNFSEGNITIDGRLDVKELYVNGTKIDTNGGG
                     NSGGNDNGWNGQYPPEVTTDRDKRYWQIWATAIGAGFTQQAAAALLGNAQGESDANPT
                     ADEGNGAPGFGYGVWQWTDSSGATSGRVYMINLMTKAGISDDPDTITAQFKLLMWHAP
                     NGQWIATSAYPYTWTQFMNLTDINTAAQAFVANFERPRDPHPERTAWSQEWYDKFKDL
                     EIPVSKGYMKPIADPITVTSEFGWRTSPITGAQEFHNGIDLVNGNPNTPIFASADGEV
                     IVAGDANYFDWYGNWTVIKHADGMYTGYAHQSRVDVSKGQKVTAGQQIGLMGTTGPST
                     GEHLHFQFMDEFYPSSSGHFHNARDYIDF"
     gene            16307..18063
                     /locus_tag="AC1ZCC_001139"
                     /pseudo
     CDS             16307..18063
                     /locus_tag="AC1ZCC_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321069.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
     gene            18080..18862
                     /locus_tag="AC1ZCC_001140"
     CDS             18080..18862
                     /locus_tag="AC1ZCC_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629405.1"
                     /translation="MLDFQSKPNIFEEMSYEEAVKWLLRQAAIHYDGSDHDAHVLATE
                     SNAGFATPETVMQARGRWLRDYKLPQKYPNILDIPPGKYATKAGWGADNPDGIEDDSF
                     VEMMVFADHDSRKLIVAFARYSGEIYIKMTHNSEPVEGYNSLGWRRVYTTSVLFEGEL
                     RKGQSVNLPDDTFRYQTLRIHYTDGDGDFVEEVKRQRYARITKANLWNGDAGVTFIEF
                     ELTIEARKITMSNGRALDISSGNVSNPAMSNNVKITKIEGVK"
     gene            18862..>19603
                     /locus_tag="AC1ZCC_001141"
     CDS             18862..>19603
                     /locus_tag="AC1ZCC_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HIQ1629406.1"
                     /translation="MAHVIKKGSIKVPTQPKDYDLQATGLVFKSYDNQIALEFNVEQQ
                     NGTPADLLGANLRLLMFIYDEVDGMITKEPIPFITKNLITESFLNGQVVYILPEAMKA
                     YNGMVEAYVYIEYPDGSTSDNLGFTFRMKRSAIDGLAQDKADYFIADFQQLLDGVKQE
                     ATDAVNAALTNVDSTIEKANQQINEFVEGATQAIDQTVDEVTEQLQATQTKIDTVSQN
                     VTSAQNNLKAVEDKMNQTNQQIGDLGKLK"
BASE COUNT         6860 a         3171 c         4304 g         5268 t
ORIGIN      
        1 ttcacaatat taatttgtca ctgtggcgga aagggtagac gctaagcatg tgtgttaggt
       61 caatgcttcg gcaaccatgc aaggttcgat tccttgccag tgactttggc aactgagggt
      121 tggaaatggg cgctcaaagt acacgagcaa ggcgaggtcg atagtaatcg atggaatcgg
      181 tgtaggttgc ttgatagaac tataactgca tctcgtttat gaggtgtagt ttttacatat
      241 tagatcactc atcgagtggt ctttttattt tggaagggga gtaaaaaaat gaacgaaaac
      301 caattaagag agttgtttaa aacgaatgaa gcaaacaaaa caatggaggc gacattctac
      361 gaaactcaaa aaagcttagc gttaatcgca aaacaagcta agtatttcta cgatcagctt
      421 attctgcaag gatttaatga cgcacaggct atggaattta tgatgcgaac cttttctgcc
      481 agtaaccaac agaaagagtg atacgtaatg aggaactact ggtatgtatc attatcaaat
      541 aaatatccgc aaccaaacgc agatgatcca attagagttg tccagtcagt ccagattaaa
      601 aagaagtact ccatcattga aatgaccaga gaagctacgc caaaagagat tgataagtac
      661 aatcttcgtt actgcggcca tggatatttt agtgagcaga acatacagac aaatattgag
      721 agatattgtt aataaaattg aaaggtggtg gcttgaatgt ggtaaatttg acaccaaaac
      781 aaaaagcttt tgcggatgaa tatataaaaa atggtggtaa tgctactcaa gccgccatta
      841 aagcaggtta tagcaaacga tcagcaagag taataggtaa ggaaaaccta actaaaccta
      901 acataataca gtatattaat gaacggctaa atcctatcga aaagaagcgc aaattaagtg
      961 ccgaggacgc tttaaatgaa ttgatagata tttggcaagg agaagtacaa ataagcgtga
     1021 gcaagcaaat agaccgcttg gagaaaaaca aggttattaa acatatgcaa tatgaatata
     1081 caccagattt agaaagcaaa ttgaaagcct tggatttgta tttgaagtat aaatcgctgt
     1141 tatcacaaac gcaattagaa aaagctcaaa cagaaataaa attaatgcaa gcaaaattag
     1201 aacaattaca gataaactca gagcgttcta ccgaagaaaa acttgatgag ttgttagaaa
     1261 agattagtgg tgaattagat ggcactagtt gatatttata acccaaagca aatcgacgtg
     1321 ttaaataaaa ccattaagaa tgattggttt attactttat tacatggagc aaaacgttct
     1381 ggaaaaacaa aaataaacaa tgatttgttc ttgtttgaat tgcgacgtgt gcgaaagatt
     1441 gccgatgaag aagacattaa ggaaccaatg tatatcctag caggagtttc aagtgcaaca
     1501 atccaaaaga acatcttaca ggaactatac aacatgtaca gcatagaacc taaattcgat
     1561 aaacatggaa actttaagct atttggcgtt aaggtcgtac aagcttatac aggaaacatt
     1621 ggcggtgttg gcgcaattcg tggtatgaca gcatatggcg cttatatcaa tgaggcatcg
     1681 ctagctagac aagaagtatt tgctgaaatc gtttcacgtt gttcaggaac aggagcgaga
     1741 atactagctg atacaaaccc tgacaacccg gaacactggc taaagaaaga gtatatcgac
     1801 aattcaagca aaaatattca atcgttccac tttgaattag atgataatac atttttatct
     1861 gaacgatatc gtaacaatat taaagaatca acaccaagcg gcatgtttta tgatcgtgat
     1921 atcaaaggtt tatgggtttc tgcagaaggt gtcgtttatc gtgattttga tgctagcaag
     1981 cactacatcc agtcaaaaga cttaccacct ttgagcaact tttattgtgg tgttgactgg
     2041 ggatatgaac actggggttc aatcgtagtt ataggtgaaa cggatgacgg aacagcttat
     2101 ttaatcgaag aacatgctac tcaatttgaa gagattgatt attgggtaga tgtggcaaaa
     2161 ggcattcaag agcgttacgg cttacgagta cctttttatt gtgactctgc gagaccggag
     2221 catgtggcta ggtttgtaag agaaaagatt aatgctaaaa atgctcataa agcacggtta
     2281 tctggagttg aggaagtcgc caagagattt aaacaagata aattgtttat ctgtcaagat
     2341 agagtgatga aatttcggga tgaaatttat caatacattt gggacaagaa aaaaggcgag
     2401 ccaataaaag aatatgatga tgtgctagac tccgttcgat atgcgatata tactcatgag
     2461 cttcttaaga aaccaaaagt taatgtcaac gaaaagatta aacgtattaa acgcatgttt
     2521 taaggagtgt gaaaaatgga taaggtaaac gagtttgaat acggagctga tatacattat
     2581 tctaatgacg tgaacacaaa ttatgtaaag tttagcgtag actcaaatct tcactataga
     2641 tttagctcag cagaagattt acttaacgat ttagatactt tagcagcaat gataaaacat
     2701 catcatgaat atcaggtaaa aaggctaagt gtattagatg attattataa agctagaaat
     2761 acaaatatca tggataaccg taggcgcaga gaaaaagaaa aagcggatca ccgatcagca
     2821 cataactttg gaaaagttct ttgtacgttt gatgttgggt acaacacagg caatcctata
     2881 aaagtgcaaa tcgaggacac aaatcaacaa aaagaaatcg aagagtttaa tactaataat
     2941 gacatagatg ggttaaatgc tgaactctgg cttgatatgg ataagtatgg gagagcctat
     3001 gagattatct atcgagattc agatgataca gattatgttg atttggctaa tgtatttgaa
     3061 acgtttgttg tatatgatac tacagtaaaa cgagagccta ttttggctgt acggtatcct
     3121 aagacaagat tcaacaagga tgctgataaa cagtacattc aaccaatcgt atacacaaaa
     3181 gaaaaaagta tcacttatga tgagacgaca ctaacagcaa ttgagttaaa gaatccccag
     3241 gatgaaccgc atgaatataa agaggtacct attacagagt attctcctaa tcgttttcgg
     3301 atgggcttgt atgaagatgt actatctttg attgatctat acgatgcagg gcagtctgat
     3361 accgccaatt atatgactga tctaaacgat gctcttctag ttattagtgg tgatattgaa
     3421 gcagcaggac tatccacaga ggacgccatc aagcagaaag aagcgaatat gcttttgctt
     3481 gagtctggaa ctgatgtgaa cggtaataaa acaagtgtga ctgcaggata tatttacaaa
     3541 caatatgatg tgaacggtgt agaagcatac aaatacagag tacgcaagga tattcacgaa
     3601 atatccatgg ttcctgatct tactgatgac aatttttccg gagtgcaatc gggagaagca
     3661 atgaaatata aattatttgg atttgaacaa atgacggcaa caaagcaaag gctattcaaa
     3721 aaaggcctta tgcggcgtta tcgtctttta tttagcctaa aatcaagtat ttctgaaatg
     3781 gataactccg atttgaaagg cttacgtgta atatttacgc ctaatctacc taaagccatt
     3841 ctggaagagt tgaaatcttt ggttgatgct ggagctgaac tcagtcaaga gacgatctta
     3901 ggactcgctt cttttgttcc agatgtacag gcagagttga aacgagtaaa taaagaaacg
     3961 caaaagcaga ttggcatttt tgattcagat ggtgaagaag taattaacaa caaaaaagat
     4021 gaaacagggg ggtgattaaa tgaactccca agaatattgg atcaaacggg aaaaggaatg
     4081 gcaaaagcaa caaattaaag atgataaaaa gcgcatggca gaaattaaaa gtcgcatgca
     4141 atacgcacaa gatgcgatac aaaaagaaat agacgcgcag tgggacagtt tctccaatgg
     4201 ccagaaaatc actcgtagcg aagcgatgaa gcgtgctagt gaaatggatg tcaaagcatt
     4261 cgctcgcaaa gcaaagaagt atgtcaaaga gaaagatttt tctcctacag caaaccaaga
     4321 attaaagcta tacaatctta cgatgcgtgt aaatagatta gagctcttaa aagctaatat
     4381 cgggcttgaa ttgatttcac tgtttaatga attggataag tacttttcga atgaattaac
     4441 aaaagctggt ttagctgaat tgaagagaca agccggtatt ttagaaatga ctattgcttc
     4501 aagtggatat gcaaagctga tagaactagt aataaacagc tcctttttga gtgatgacgt
     4561 gtcttttagt gatcgcttat ggatgtatca atctgaattg aaatcagaat tagataggtt
     4621 gttagtcaga agtataacga tggggaaaaa tcccaagcaa cttgcatcta aattggcaga
     4681 atatttaaca gctgaaggac gagaaaacac taagttcaac actcaacgtt tgatggtgac
     4741 tgaaacgact agagttcagg tagggattca agaacgaagt tatagagatg caggcattac
     4801 ccagtacatc tatatagcag aaccaacggc gtgcaaacta tgtataccgt taaataatca
     4861 agtttttgat gttgccgata tgcagccagg aagtaacgct cctaatatgc atccattttg
     4921 tcgatgcagt acagcacctt atatagaacg aatatcaagc cgttaacaca aattaacggc
     4981 tttttattgt gccttcttac agcttacagg cgttaaagag aaagctattt tcggctgacc
     5041 ggcgtaactg gtcaaattta tcgggtagcg gcgtaaccgt ggaggattaa tcatgaaaaa
     5101 acgtttattt atgccaatga acttacaatt tttttctgaa ccaggagatg gtggatctgg
     5161 tgatgaggga caacaaggaa acctaccagc tggctcacaa gagacaccgc ccgaagcaaa
     5221 agaagaaaac aatactggca aaacattttc tcgtgatgaa gtagcgaaaa tgatcgctgc
     5281 tgagacgaat aaagcaaaag cagcgtggga aaaagaacta gcagcaaaaa aagaagaagc
     5341 taaaaagctg gcaaaaatga atgcggaaga aaaactacag catgagttgg agcaaaaaga
     5401 agctgaaatc gctgaattaa agcgtggaca ggcactatct gaaatgacga aagaagcttc
     5461 taaaatgctg acagatgcaa atttaccaca cgatgatgat ttacttggtc tgattgtttc
     5521 tgatgatgca gatgccacaa aacaagctgt agcagtcatc actaactttg cttctttgat
     5581 taagagagaa aacgcaagac aaacaccacc aaatgaaggt ggacaattta cagcatcgaa
     5641 aaatactaaa gaaacagtgg ctaaactagc tgctaaaaat cgaattatca aataggagga
     5701 aaacttaatg aaaaagaaac aacttttacc aatgaacttg caaatgtttg ctcaaacatg
     5761 ggatccagat aatgtcttgg tatatgaaac gaaagaggga aaaattcctg ataaatataa
     5821 tacgctcatt ttgagtgaag ttatggaaaa ttctaagatc atgcagttag caaaatacga
     5881 agaaatgact gacaaagaaa agaaatttga atactttgca gaaggaccag gcgcatactg
     5941 ggtgggtgaa ggtgaaaaaa ttaaaacgtc taaacctaaa tggatgcaag ctacgatgac
     6001 tgcaaaaaaa ctcggtgtca ttcttccggt ttctcgtgaa tatttaaatt ataaattatc
     6061 agatttcttt gaggagatgc agccaaaaat tgctgaagct ttctataaaa aatttgatgc
     6121 agctgcctta ttaaataaag aaaacccatt tcctcagtca ctagacggat cagttattag
     6181 tgcggggaat gtggttgaag gcggattgac ttatgataat atcctagcct tagaagacaa
     6241 gttagcagaa aatgaattcg aacctaatgc gtttatttca aaccgaaaaa atcgtacaga
     6301 attacgttct gcagctcaaa cagtcgggtc aaatgttgag tttatttatg atcgctctac
     6361 taatacaatt gacggattac cagtagtaga ccttaagtct ttagataaag gaacacttta
     6421 cgctggagac tttaattaca tgttttatgg gatcccatat aatatttcat ttaagatttc
     6481 tgaagaagcc caattgtcta ctttaactaa tgaagatgga accccagtta acttgtttga
     6541 gcaagaactg attgctttgc gtgcaacaat ggatgttgga tttatgattg taaaagatga
     6601 agcatttggg aagatttccc caaaagcgta acgcctgcta ccggtattgt gccaaatcaa
     6661 aagacatgga ccggtaaagt aggcgatact aaaacattta ctatttcagc tgtgcctgca
     6721 gatgctagcg atgcagctgc tgttgttgca gctactacag caacttcaag tgatggagct
     6781 atcgcaacag tgaccaaaaa tgaaaatggt ggttttgatg gaacgattgc agcagaaggg
     6841 tcagcaacat tcacatttac ttctggagaa ttcactactt caatcaatgt gacaggtcaa
     6901 cctgctagtt aggaagtaaa aatatgatga ttgcagatga tattaaaaaa cttcttaaag
     6961 gaacaataga tgaaaagctt gaagttattg agcgaagaac gaatgagcgt atgaaaacct
     7021 tgttaaatac gcaagaagtt cctaaagaat ttgaaacagt tgtatatgaa gtatcgttga
     7081 aaagattcaa tagaattggt caagaaggta tgcagtcata ttctcaagaa ggtttatcta
     7141 tggcttttcc tgactcggat ttttcagagt atcaaaatga gattgatgaa tttaagcgta
     7201 aagatcagga agagttgtac aagccaaagc gagggaggtt taaatttgta tgagatttac
     7261 agatgaaatt atatttgtta aacgttcatc tgactctaaa tatgatccag atctcggtga
     7321 gtgggttgaa ggcaaaccag aaagaacaag aacagaggca aacgtgacag atattggcac
     7381 tgatagaagt gtgactattt ttggtagtgt ggaagaaggg gcgaaggtca ttaggacgca
     7441 gcctcttttt tctatcccta catttgacta tatcgagatt gaaggaaaga cttggcaaca
     7501 aaaaacagct agaaatccag catatagaaa tagtttaatt gtgcaagagg tggttcttga
     7561 tgaaggcaca acttgaatat aaaggaatcg atcagctgat gcgacatctg aaaaaagcag
     7621 caacgcttaa tgacgttcaa aaagtcgtga aaagtaatac tgctgaaatg actgaacgaa
     7681 tgcaaaaagg tgcgccagtg gatacaggtt acttacgaag atcaataaac atgaatcttt
     7741 tagaagctgg tttaactggt attgtaggac cgacagcaga ttatgcacct tatgtagaat
     7801 atggcactcg atttatgtcg gcacagcctt atgttagacc agcgtttaat taccaaaaag
     7861 tcaaatttat ggctgaaatg aaagccttgg tgaaatgatg attaagacaa gagatcaatc
     7921 aatttttgat gaacttttta aaatatccca aaacaaactt ggatataaaa catacgatta
     7981 caaaacttta gaggatgttg gttatccttt tgtggaattt gagaacactc agacaatcca
     8041 tgaagtaaat aaaactgaca ttaaagggtc tgtgattgtg gttttatccg tttggggatt
     8101 acagaagaaa cgaaagcagt tgtcagatat ggcatctgct ctttttaatg aagctagatt
     8161 gatagaagcc acagaaggct attattgggc tttaaattat caagcaagtg gaattcaagt
     8221 gatggacgac acaacaacga atacaccgct aaaacgggcg gttgtcacac ttgaatttag
     8281 aattagatag gaggaagaac atggaagcat taaaaggtat tgatgtcatt ttgctttatc
     8341 gcttattgaa aaaagaaact caggaagccg cttggaaaat ggcatttcaa acagaacacg
     8401 aaaatggttt atcaagagat tcagactcta cagtgacaaa agatggaaac gttcaaagtt
     8461 taagcccggt tgaatatgat ttttcggcta cttcaatagt tgctaaagga gattctcatg
     8521 tagatgaaat gaaacaagct ttattaaatg gggatatcat tgaaatttgg gaaattaata
     8581 aagcagaaca gggaacagat gataatgcaa ataagtacaa agctacttat taccaagcat
     8641 atgtgtctga atttactcca tcggctgctg cagaggataa cgttgaatta agtttatcat
     8701 ttgcagtaaa tggtgttggt caagatggtt atgcaacctt gacagaagat caagccgatg
     8761 ttgttcaata tgcattcaaa gataccgtga aagcaacttc gacaggagca taagagggct
     8821 tagatgctct cttttttatt ttaggaggat gaaaaacatt gaaattaaaa attaaaggta
     8881 aagaatattc gtttaaattt ggcactaaat ttgtacgtga attagataaa gtgatgcctt
     8941 tcgtcgatgg aaatatggaa tttggaatgg gactctccgc aaaagtctta ccagagctac
     9001 gttcttataa cgttaacacg ttatcacgag ttctggaaat agcaaataga actgaagatg
     9061 aatctattac attagatgaa ttggatgatt acatcgatga agttaaagac attgaaaaat
     9121 tgtttgatga agtcctaaaa gaattggcgg agtcgaacgc gggaaagtta gcggtccgaa
     9181 acctgaatca gaaattgaaa gaagcggaaa aacaacaagt ggaatagatt ctgcactggc
     9241 atacgaacaa attcttatca attcttttcg atatttggga atgaccaata tctcagatat
     9301 cgaaagaatg acgttatatg aatacaacat tcgtatgact gcagcccagt tatcttggct
     9361 tgacaaagaa aagttgattc acgaattagc gtgggcaaat cagcaagtcc aagcggagaa
     9421 aaaagtaggc aaaaagacag ttcctgtata tcgatccttt gaagaattct tcaattatca
     9481 aaaaatcgaa gattcaatca tgggagtttc cgaactttca aaacaagata aaaaattcca
     9541 aagcttacta actaaagcta actcttgagg aaaggaggag aatcatggaa caattttctg
     9601 ttgaagcctt attaaaagcc acagatagtg gatttgtaaa gacttttaaa gatgcacaag
     9661 atgctgttaa aacttttgaa aagaattcaa atagtatgac aaccgctgtt ggtaaagtga
     9721 tgcaaggtac tggtgccgca atgacaaagt atattaccac tcctcttata ggagtaggcg
     9781 tagcagctgc taaagttggt ggcgacttcg aagcacaaat gagtcgtgta aaagctatat
     9841 caggagcaac cggcgacaca ttcgaacaga tgaaacagca agcgattgat ctaggagcaa
     9901 aaactgcttt tagcgcaaaa gaatcagctg ctggaatgga aaacttagct tctgctggat
     9961 ttagcgcaca agaaatcatg aaagcaatgc cgggtctttt agacttagca gctgtatctg
    10021 gaggggatgt ggctctagct tctgaaaata ctgctactgc tttgagagga tttggtttag
    10081 aagcaagtga agcaggacat gtcgctgatg tatttgctcg tgctgctgcg gataccaatg
    10141 ctgaagttgg agacatggga gaagcattga agtatgttgc tcctgtagct aattcaatgg
    10201 ggatttcttt ggaagaaact gcagcagcta ttggtattat gagtgacgca ggcattaagg
    10261 gttctcaagc aggtacaacg ttgcgaggag cattgtctag gttagcaagg ccaacaaagg
    10321 ctatgcaaga tacaatggat aatttaggtg tttcgtttta tgatgctgac ggtaaaatga
    10381 aacctttaaa aactcaagta gaattactta aaaaagcttt tgaaggcctg acgcctgaac
    10441 aacaacaaaa tgctttagta acactatatg ggcaagaatc attatcaggg atgatggctt
    10501 tgattgataa aggacctgat tcattgggca aattaacaaa atctctgaaa gattctgatg
    10561 gtgcagctga cgatatggct cggaccatgc aagataatat gaattcttcc atcgagcaaa
    10621 tgtttggagc ttttgagtca gcagctattg taattcaaaa gattctagca ccatccatca
    10681 aaaaagtagc agatgccata tccggcttag tagaaaaatt tgtaagtgct ccagaatcaa
    10741 ctcaaaaatt agtagtggcc ataggagcaa ttgtcgctgc tataggaccg ttaattttta
    10801 tgattggttc agtaattata tggatcaata gggtgaaagt agcttttaaa gctttaagtg
    10861 aaagttcaaa attgtttagt ggattaagta aagcaatggg tcttcttaca aatccggttt
    10921 ttctggttat agctgcggta gcactactcg ttgtaggttt catctatctt tggaatacga
    10981 gtgaagattt tagaaacttt tggattggct tatgggaggg aatcaagtct gctgtaagct
    11041 cggcagtaga atggattcag aatgcatgga aatctacagg agaatggttt aacaatttat
    11101 ggaagtccat taaagaaggc gcagacaatg tttggactac agttcaagaa gcccctggga
    11161 aagcggcaga ttggattaaa aacaaatgga ctggaacaaa agagttcttt tcaaatttat
    11221 ggtcaagtat tgcaaactct gcttcagaga tttggaatag tttaaaagaa ggtgtcatat
    11281 cagttattga tgatttagtt tcaagtgctg gtgaaaaatg ggaagggttt aaaaatacta
    11341 tatctactgc atggaaaaca attacaagta aaatcaaatc tggttttgat tttatactaa
    11401 aatatattgg tccatttgta agtagctttt cagatgtgtt ctctaatata gtgaaagcaa
    11461 taacaagtat atttgctgag gttaaaaaca taatagtaaa tgcttgggaa atcattaagt
    11521 ctttaatagc tgcgccacta ctgtttatta tagatttaat tactggtgac ttcgaacaaa
    11581 tgaaagagga tttagatcta atctggaaca cacttgtcca atcagtggta aatatttgga
    11641 catctgtaaa aaatatattt acggaatata tcggtgcaat agtaaatagt gccgttagtt
    11701 tatggactgg attcatacaa agtatttcta atatttggaa tgaagtagtt tatcaagcga
    11761 ctatgatttg gattgatttg aaactatttt ttactaattt atggattgat attaaataca
    11821 gtgcaattca aatgtggata aatctaaaat tctccataat tcaaacttgg attgatacaa
    11881 aatatggtgc aattgaactt tggaataatc taaaacaatg gtttttccaa acggttaata
    11941 atatcgtgca aactcttata aaaagttgga acagcttaaa gcaaggaacg atagatttat
    12001 tcaacaatac agttcaaggt gctaaagaca tttggacttc attcaaatct tggattggtg
    12061 atttaattac tggaaccaaa gataacatca ttcagggatg ggaaaaccta aaacaaggta
    12121 ctatagatac tttcaacaat ttagtaaatg gtgctcaaga ggcatgggat aatttagtaa
    12181 atgctgttag tgacacggtt gatagagtaa ctggctggtt tgataacttg aaaaatatcg
    12241 atttactagc agccggaaaa gctatcatgg atagttttct agaagggtta caaaatgcat
    12301 ggaaatctgt gcaagatttt gttggaggta ttggtgattg gattcgtgaa cacaaaggac
    12361 ctatccaata cgatagaaag ctattgattc cagctggtca ggctattatg aacggtctga
    12421 ataaaggtct gacaggagga ttcaatgacg tacagaatac tgttggaagt atggcagact
    12481 ttatcgcgga acttttcaat gcaaattctg atgtagatat agctgcaaat ttgaaaaatg
    12541 caaataaaaa cattggtgca caagttgaac ataaaataaa tatgggtggc tctactaaac
    12601 cagctgtatt taaaatcaat cttggaagac aatcgtttag attgtttatg gacgatattt
    12661 cacaagctat gggcgaaggt gcagatatta atctggaatt ttaggaggga atattttgga
    12721 tcaacgagaa aataaaatgt actcattcaa agatacaacc attaatctca ctagttctaa
    12781 acgattcctt ccgacgtctg ccatgatgta cgatggaatg tatttagaag atttgattga
    12841 tggttatcaa acacttacgg tggaaggtag agaaatgctt tctgtagaag ttgaacagca
    12901 agagatacaa attggttcaa tcattacaaa tcagaaaata ccttcaagaa cactaaaaat
    12961 aacatataag ctggaagata gagatccaga aaaactacag tttaaattca aagaactgtt
    13021 gaattattta taccggaatg aagacgtgga aattaggttt catgatgaat tagattatta
    13081 ttactacggt cgctatacat caactgatac tgttccagga gactccaact cgattatttc
    13141 gagttttaat gtattctgtg cggatccact aaagtatacg aaagagtgtg ttagtgatgg
    13201 ctatattgga aatccgatac agtttcctat aacaccaaga aaaattgaag ttactttatc
    13261 catgaataat tcaatcaaaa ttacaaacgg agaacaaaat atcacgataa ctgacgcggc
    13321 aataaaaaca ggagacgtgt tggtttttga tttttccgat gagcaggtaa ctgtaaacgg
    13381 agaagattgt acttctatga ttgatttaga aagtgatttt gagaactttt atcttaagca
    13441 aggtcagaag ataactagca ataatgggaa gcttaaaata ttctataggg gggcgacaat
    13501 ttgagtgaga cagtttattt ctttgatcac ttgcaaaaac ttattaaaag aaaaaataca
    13561 agaagtttga ttgaagtctc ccaagaaaaa gaaattagtt ctgataagag tgatctaatg
    13621 aaagatactc tttacgttac gacaaaatat gataaagaaa tagaggatgc aagatatatg
    13681 gcgattcgtg aaaacgagtc gtctttttcg ttgtatcgaa ttactaaagt tagcgaccca
    13741 tttgaaacat tagagtttac agggttagga tttgcgacaa atgaattaga tgcttacatc
    13801 atcaaagata ttaggccgag tgggcagccc ttaaaaaatg ttcttgatcg attgattgaa
    13861 tttactgaag gaaattggcg cgttggtcac gtagaagcaa tgttaccaac agtaactgca
    13921 actttttact atgtctctgt aaaagaagcg ttgaaagaat tgcaaacctt aggtatggaa
    13981 tttgtcttta ggtgttcttt gaattctgat ggaataaagg ataaatggat cgaagtatat
    14041 gaacaaattg gcgaagaatc gaatacacgt ttcgtatatg gtagtaaagc attaacagtt
    14101 gtaagagaga tagatagaag ctcaatctca acttcaatga taggtcgtgg gcgaggcgaa
    14161 gaggttggtg acggttacgg tagaagaatt gaattcactg atgttgaatg gaaaaagtcg
    14221 aatggtgatc ctttagataa gcctaaaggc caaaattggc ttgaagatcc ggaatcaact
    14281 caaaagtatg ggataccaca aaaagatgga tcaatgagaa aacgagaaac cgtagtagtg
    14341 tttgatgata tagatgatcc aacagattta cttaaaaata cttattcaac cttaatcgat
    14401 tctgctagac cgttagtaca attcaaagct gaagtcactg gaggagatgt gataggaaat
    14461 acagtgacta ttcacagata cgataaaggt tatcactata aaactcgtat ttataaaact
    14521 acattcaatc ggcttaccgg tcaaacgaat atcgaactag gggataattt aacacaagat
    14581 gttagaaaac aaacggcttc tattgtcaat aatattaata gtttagaatc tagcaaaatg
    14641 acattttacg aatcgacaga gattggaaaa tatcaagatg acattatgcg aggtgcagga
    14701 gataatggcg gttctattta ttgggtaaat ggaattgaag ctggtgttag tgatagtaga
    14761 gaaatctatg aaactgttta tatggatgga cctaacattc ctagatcacg cttttttatg
    14821 gtccaaaata actcagggat atctttcaaa caatgtaaaa aaggtgagtg gcaaacaatt
    14881 caagatgtac acaatggtga tagcacgacc gcttggacgt tggatggaac tttcaatgct
    14941 aattttattc gcgcaggcat tctgtctggt atcctcgtgc aaggggtagc tttaaaaaca
    15001 tttgacgata aagatttcca attagtggca gaggggggag aactttcttt tgaaaaacaa
    15061 gtcaagtcta ctggactaga tgatgtacat ggagaacgct tgggctcaat tgtatctact
    15121 tatggttcta aagggattaa cggctttgct gtctggaaag agccaaacta tattttttcc
    15181 attaatgctg gggacggtgg cgatcgaggg aatcctattt ttcaaattcc agcagatgtt
    15241 actgctgata agcgcaaata taatctttat ggtgacggca atttttcaga aggaaatata
    15301 accatagatg gacggctaga tgtaaaagaa ctctatgtta acggtacaaa aattgacact
    15361 aatggaggag gcaattccgg agggaacgat aacggttgga acggacaata cccaccagaa
    15421 gtaactactg atcgggataa acgatattgg caaatttggg caacggcaat aggtgccggt
    15481 tttacacaac aagctgctgc agccttactt ggaaatgcac aaggagaatc agatgctaat
    15541 ccaaccgctg atgagggcaa tggcgcacca gggttcggtt atggtgtatg gcaatggacc
    15601 gattcttctg gcgcaactag cggacgtgtc tacatgatca atttaatgac aaaggctggc
    15661 atcagtgatg atccagacac aatcacggca cagtttaaat tgttgatgtg gcatgcacca
    15721 aatggtcaat ggatcgcaac tagcgcttat ccttacacat ggacacaatt catgaatctg
    15781 accgatatca acacagcagc acaagcattc gtggctaact ttgaacgtcc acgtgatcca
    15841 catccagaac ggacggcatg gtcacaagaa tggtatgaca agttcaaaga tttggaaatt
    15901 cctgtatcaa aaggatatat gaagccgatt gcggatccaa tcacagtgac gagcgaattt
    15961 ggctggcgca cttctccaat cacaggagca caagaatttc ataacggtat tgaccttgta
    16021 aatggaaatc ctaatacacc tatttttgct tcagcggatg gcgaagtgat tgttgcaggt
    16081 gatgcaaatt actttgactg gtatggaaat tggacagtga ttaaacacgc tgatggaatg
    16141 tatacaggat acgcgcatca aagccgagta gacgtctcaa aaggtcagaa ggtaactgct
    16201 ggtcagcaaa ttggactgat ggggacaaca ggaccatcca ctggagaaca tcttcatttt
    16261 caatttatgg atgagtttta tccatcttct tcaggtcatt tccacaatgc aagagactac
    16321 atcgatttct aaaggaggga tagtcatggc agaaacgcag cataaaatgg tcctatccac
    16381 caccgaacca aacaacggaa taaatttggt tcgaattcgg caaggggatg ttttaacgca
    16441 aaagttcgtt gttgaagtgg tggaacatgg caaactaaaa acattcgagg gcctagtgcc
    16501 gttttttatt aatacaacaa aatttggcga aaaccaacct gttgaacaaa aagtacaaga
    16561 atacagtcca gcgcaagcaa ggcttgttta caccttaagt gagcctgact ggcaatgggg
    16621 tggtgaaaac accgcacatt tcagtttccg atcacttaat ggtgatggaa cttggagtga
    16681 acaatttagc acacaggatt ttacctatcg agtcatttct ggaatatcta gaagccagtt
    16741 acgtgactct ggctatgtgt ggacatttga ggatttgcta agaaaattca aagattacat
    16801 ggatcagggc aaaaatgact ggggcagtgg ttagaagata atcgtgaaat actggaaaat
    16861 atcgatccag gtggtatgat cattaacatt ctgaatgaag ctaaaggcga ctacgaatca
    16921 ttagctgatc gtttaaacca aaaataccaa gtgccagtcg gcagctcaca aattagagaa
    16981 acaacacgct tttttgatta cgacacgatg aaatacgttg acctagtgcc gcgcaatttg
    17041 aatacggttg tcaacagtgt taataacagc aaatttaact tttctttcat tacagacatt
    17101 cacgcagata atcacaactt gcgtatagat ggcgtcggtt acaaagatgc ttcttattta
    17161 agacattggc gcgcaatccc tcaatttcaa aaattaggga acaaaacaga tgtgatgatt
    17221 tacggcggag acaatatcga cggcggactt ggttcactcg gcagcgatat aggcattatt
    17281 gatgaatgga gcgcgcgaca ttccatgtta ggcacgctca aacgcttcac taacgcagcg
    17341 gtagcaggac aagaaaaacc ggttattatc tgcaaaggga atcacgacgc ttgtttcgac
    17401 cccgcgtggc gcaagcgaaa gggaatgtta tgcaacgctg acttcgaaga gtattggaac
    17461 ggtttgtacg gtggcgtgtt gttcccagat aaaaacgcag caatttaccg tttcgatact
    17521 tgtgattttt atgaaggcgg cacaggcgac aagtacgtgg acggttacag cgatacagcg
    17581 ccgggagctt tcagtgccaa acaaattaac gctttcggag aatggttagt gaacgttccg
    17641 agaaactatc atgtggcgtt agtaggacat acgcctttag gtctcagcaa gtttcccgtt
    17701 cgcaatgaaa acatgattag cacgttgata gaagggttta aatctggtag tccggtaact
    17761 atcgattggt ctaaattagg acaaccgaat gacggttcgt tcggcggatt aaagaccttc
    17821 gcaatgaata caaaaggcgc cggcgttgta gtaggttatt tctgcgggca ttggcacgaa
    17881 caagtcgaag gggcgtttgg aacagtaaaa atgattcttt gcgacgtcgg cttctctcat
    17941 acagctagcc aagttgatac accggatgaa ttagcgtttt ataaaataga agttgacaca
    18001 gcaacaagaa aagtgacaag caaaggcgta gggcgcgcga gagacttcac ttataactat
    18061 taaggcggtg aacaattaaa tgttagattt tcaaagcaaa ccaaatattt ttgaagaaat
    18121 gagttatgaa gaggctgtga aatggttatt gcgtcaagcg gctatccatt acgacggctc
    18181 ggatcacgac gcgcacgtcc tagctactga aagcaacgcg ggctttgcta caccggaaac
    18241 agttatgcaa gcgcgtgggc gttggttacg cgattataag ctaccacaaa aatatccgaa
    18301 cattttagac attccgcccg gcaaatacgc aaccaaagcc ggatggggcg cagacaaccc
    18361 tgacgggatt gaagatgaca gttttgttga aatgatggta ttcgcggatc acgattcgag
    18421 aaaattaatc gtcgcttttg ctcgttatag cggcgaaatt tacatcaaaa tgacacataa
    18481 cagcgaaccg gtagagggtt ataactcgtt aggctggcgg cgcgtttaca ctacttctgt
    18541 tctttttgaa ggcgaattaa gaaaaggaca atcagtcaat ctgccagatg atactttccg
    18601 ttaccaaacg cttcgtatcc actacacaga cggtgacgga gactttgtag aagaagtgaa
    18661 acgtcaaaga tacgcgcgaa ttacaaaagc gaatttatgg aatggagacg cgggagtgac
    18721 gttcatcgaa tttgagctga caatcgaagc gcgaaagata acgatgtcaa acggcagagc
    18781 tttagatatt tcatccggca atgtttccaa tcccgcaatg agcaacaacg tgaaaataac
    18841 taagattgaa ggtgtgaaat aatggcgcat gtcatcaaaa aaggctctat caaagtacct
    18901 acacagccga aagactacga tttgcaagca acggggcttg tatttaaatc atacgataat
    18961 caaatagcgt tagagttcaa cgtcgaacaa cagaacggca caccggcgga cttgctagga
    19021 gctaacttgc gcttgttgat gtttatctat gatgaagtag atggaatgat cacgaaagag
    19081 ccaatccctt ttatcacgaa aaacctcatc actgaaagct tcttgaatgg acaggtcgta
    19141 tatatcttgc cagaagcgat gaaagcttat aacggtatgg tggaagctta tgtttacatc
    19201 gagtatccag acggatcaac aagtgataac ttaggcttca ccttccgtat gaagcgttca
    19261 gcaatcgacg gactagcgca agataaagca gactacttta ttgcagactt tcaacaatta
    19321 cttgatggag tcaaacaaga agcaacagat gctgttaacg cagcattaac aaatgtggat
    19381 tctacgattg aaaaggcaaa ccaacaaata aatgaatttg tagagggagc cacacaagca
    19441 attgatcaaa ctgttgacga ggtaacagag caactacaag ctactcaaac taagattgat
    19501 accgtttctc aaaacgttac atcggcacaa aacaatctta aagcagttga agacaagatg
    19561 aatcaaacca atcagcaaat cggcgacctc ggcaagctga aaa
//
LOCUS       DBNICI010000039        19586 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.039, whole genome shotgun sequence.
ACCESSION   DBNICI010000039 DBNICI010000000
VERSION     DBNICI010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.039"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            62..361
                     /locus_tag="AC1ZCC_001142"
     CDS             62..361
                     /locus_tag="AC1ZCC_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629407.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKK"
     gene            402..1094
                     /locus_tag="AC1ZCC_001143"
     CDS             402..1094
                     /locus_tag="AC1ZCC_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HIQ1629408.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            1091..1858
                     /locus_tag="AC1ZCC_001144"
     CDS             1091..1858
                     /locus_tag="AC1ZCC_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HIQ1629409.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            1958..3526
                     /gene="liaX"
                     /locus_tag="AC1ZCC_001145"
     CDS             1958..3526
                     /gene="liaX"
                     /locus_tag="AC1ZCC_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HIQ1629410.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            3823..4143
                     /locus_tag="AC1ZCC_001146"
     CDS             3823..4143
                     /locus_tag="AC1ZCC_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIQ1629411.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            4143..4493
                     /locus_tag="AC1ZCC_001147"
     CDS             4143..4493
                     /locus_tag="AC1ZCC_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIQ1629412.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4660..5598
                     /gene="hprK"
                     /locus_tag="AC1ZCC_001148"
     CDS             4660..5598
                     /gene="hprK"
                     /locus_tag="AC1ZCC_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HIQ1629413.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5613..6443
                     /gene="lgt"
                     /locus_tag="AC1ZCC_001149"
     CDS             5613..6443
                     /gene="lgt"
                     /locus_tag="AC1ZCC_001149"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIQ1629414.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6487..7512
                     /locus_tag="AC1ZCC_001150"
     CDS             6487..7512
                     /locus_tag="AC1ZCC_001150"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIQ1629415.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7527..8465
                     /gene="galU"
                     /locus_tag="AC1ZCC_001151"
     CDS             7527..8465
                     /gene="galU"
                     /locus_tag="AC1ZCC_001151"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-alpha-D-glucose metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HIQ1629416.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            8590..9030
                     /locus_tag="AC1ZCC_001152"
     CDS             8590..9030
                     /locus_tag="AC1ZCC_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HIQ1629417.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            9033..9635
                     /locus_tag="AC1ZCC_001153"
     CDS             9033..9635
                     /locus_tag="AC1ZCC_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HIQ1629418.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(9703..10809)
                     /locus_tag="AC1ZCC_001154"
     CDS             complement(9703..10809)
                     /locus_tag="AC1ZCC_001154"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HIQ1629419.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILSGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            11019..12020
                     /gene="ccpA"
                     /locus_tag="AC1ZCC_001155"
     CDS             11019..12020
                     /gene="ccpA"
                     /locus_tag="AC1ZCC_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HIQ1629420.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(12356..14701)
                     /locus_tag="AC1ZCC_001156"
     CDS             complement(12356..14701)
                     /locus_tag="AC1ZCC_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIQ1629421.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            14980..15882
                     /locus_tag="AC1ZCC_001157"
     CDS             14980..15882
                     /locus_tag="AC1ZCC_001157"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HIQ1629422.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            16095..17870
                     /locus_tag="AC1ZCC_001158"
     CDS             16095..17870
                     /locus_tag="AC1ZCC_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HIQ1629423.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            18127..19344
                     /locus_tag="AC1ZCC_001159"
     CDS             18127..19344
                     /locus_tag="AC1ZCC_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HIQ1629424.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLCSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFLNRLTPYALILIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKEALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(19354..>19586)
                     /locus_tag="AC1ZCC_001160"
     CDS             complement(19354..>19586)
                     /locus_tag="AC1ZCC_001160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629425.1"
                     /translation="MFLTWLDLLPQMQAFEHRLFGKRNCDKTFVTVPFPTISQVKGVR
                     KIAHKRKRQNQGTPNKAPFVKNFRSGQRSGIL"
BASE COUNT         6275 a         3543 c         4025 g         5743 t
ORIGIN      
        1 aaagggtttg tgacaaaaca tgtcacaaac ccttttcatc atttcataga aaggataaag
       61 aatgcaaaaa aaaattagca tcatcactat attttttatt tgtgtgctta cttcatgggt
      121 tattcagcat caagctgctt tatatgaagt acctgtcatc gagatcgaaa aagtacgaat
      181 aaaagaggat catcaattga ttacaggaag actgatgaac acggatcaaa aaggagaatc
      241 gatttattta tcggttcctt atcaaaaaaa tcaaatagaa acagcaaata taaaagcgag
      301 acaaatgtat ctagtccaaa tgcatggaga tgaatggcag ctagtagcaa aaaaaaaatg
      361 atggctggct tttcttcttc acaagtatct ccattggcgc catgttgctt attggcggaa
      421 aacagggtct atttgcttta ggaagtctca tcatcaatat cgttttactt ttacttttgt
      481 tgctcatctt tactaaaaca gaagggattt ctctattaaa tatatctatc ctgttcgtcg
      541 ttatagggat gattattagc atattggctt tagatggatg gaatagaagc agtataatca
      601 aaatttcagc tgcagttacg agtgtttttt tagcattttt tatttgtctt ttgacaatga
      661 atcattggaa agaccaagga ctgcgctatg aggaactgaa ttatctgaca cgaccgtatc
      721 gttctgtttt tctttctagc gtactgattg gaacagctgg aggaacactc gataccgtta
      781 tcacagtcat tgcgacatta gaagaattga ctaagcgaca accagaaata acagcgaaac
      841 agttatggct ttcaggaagg caagtgggtc gagatgtgat tggctcgatg accaatatcc
      901 ttttgttcgt ctatatcagt gggtcagttc catcgcttct tttatattta ggaaatcaat
      961 ggtcgttcaa agaaacgata gaacagcatt tatcattgga atttttacgc gtcattgctg
     1021 gcagcttagg aatagtatta tcgatcccta tctcggtcct cttttttctg actgtaagga
     1081 ggctgaaacg atgaatgtac ttattatact tgttgggatt ttagcaatca gcgtgctact
     1141 tattggcggt acgcaaggta tgtatattct tttaggctta ttcatcaatt tagggatctt
     1201 tttcctgttg ttattcggtt atcatcagaa gtggccgata ctggcattgt cggtcatagg
     1261 atttttattg attgctgtgg ttatcttatt ttttatcaac ggttataatc taaaaatgcg
     1321 tgcagctttt gtgagtatac tgatcttttt attttgtttc ctactattgc ttcctatcac
     1381 tgatttttta gcgatccaag gttttacatc ggtagaacta gaagaattat ctggcttaga
     1441 taagacttta gcgattgatt ttagattact tacgagatcg ttgcttctga ttagtcttag
     1501 tggtgctgtt ttagatgcta gtgtagcaat ttctagtggg acattcgaag tatatcaggc
     1561 aaatccgcaa ttatcttttt accaattgcg ccattctagt ttcgctattg ctaaaaaaat
     1621 attggcgtca acagtaatga cacttctttt tgcattcatg ggaagttctt tagcgttgat
     1681 cctctggttt attgatttag acattccttt tcagcaagtg atcaatgaaa aatcatttgt
     1741 tctagaatat acgatggcta ttcttaccac attgtctgca ctacttgttc ttcctttaac
     1801 ctgcgtgact acagcatatc tctttacaaa aaagaaagaa caactcaaga tggagtaaaa
     1861 gttacaactt aagtcctatg acatttttac tgaatcagtg cataatatgg atgtaaggaa
     1921 aagcttgcaa acaattttgc ttaaggaggc aatttccatg aaagaaagag aacgtatttt
     1981 agatttagtg aaaaaaggaa tcctttcgac tgaagaaggc ttagatttat tagaaagcat
     2041 ggcaactgaa aaagacgaaa agcaaataaa aaaagaagca gacaaggtaa ctgctagtca
     2101 taaagaaaaa gatcaggcaa gtcaattgat cgataaactt gaaaacggcg aagaagaaat
     2161 ttctgagcca gttgatccaa aggaaagaga aaggcaagat caagaaaact tagaaaagat
     2221 cttagatgaa ctagcaactg aagcgaataa aacttcggcg cgtctagatg aagtaaatgc
     2281 tcaattcgct gacaataagg cagcacgcaa tgagaaacag gaagcattga tgcagctgaa
     2341 tacaaaagaa gaattaggcg agttgacaga agaagaatta gcacagcgtc agacgttaga
     2401 agcagaaatc aaagaattag aagctagtgg cgatacactg ttagaagaac aattgaaatt
     2461 agaagcagaa ttaaaagata tccgtaagaa tcaatggtct gagaaaaaag aaacgtttac
     2521 tgataagttt gaattgccag atgattggaa agatcaagca acagatacac tgaatcaagt
     2581 aggcgaaaaa atgtcagaag ctggatcaca gttaggcaaa ttcttgaaga aaacattcca
     2641 aactgtttca gaaacagtga atgacaacat ggaatggaaa gatgtcagct tacgtgtccc
     2701 tgggattgcg acaacgaaat ttgagcatga attttactat gaagctcctg cagcaagtat
     2761 ccttgatatc aaggcagcaa acggaaacgt gacgttaaaa acgtgggaca gcaacgatgt
     2821 gaaagttgaa gctaagatca aactttacgg aaaaatgggt gctgaaccat ttgaagcatt
     2881 ctctgaacgg agccagattg aggttaatga agatcatatc tccttccaaa ttccaaataa
     2941 acgtgtgcgt gcagaccttg ttttctactt acctaaacga gtatacgacc acgcagctat
     3001 taaacttcta aatggcaaca tcatgattga aacattggaa gctaaagata tttatacaaa
     3061 atctacgaat ggtaacatca tcgtggacca attaactgca acgatgctgg aagtagaagg
     3121 tgtaaacggc aatatcgata ttcgaaacgg gaacatctta gattctatta tcgaaacagt
     3181 aaatggtaca gtgacatttg gtgcgacagc agaaaacctt tctgtgtctt tggtaaatgg
     3241 tgatgtacgt cttacgatta aagaagataa tttgaaaaaa gtagaagcaa gttcagtgaa
     3301 cggaaatgtc aaagtcgctt taccagatgg catcggtttg gaaggacatg ctaaaacaag
     3361 tcttggaagt atcaacagcc gtctgtccaa ttacgaagta gttcgtgaga aaaaagaacg
     3421 gacaaaccaa atgttgcaat tccgtcgtgt ttcagatggt gaaatcacgc aagtgcaact
     3481 ttcaacgaca acaggaagta tctatttaaa agatacagat aaataaagag gttgtgaaag
     3541 agatgttcag ccttgagcaa taagaagcga ttttgtaaaa tagcttcccc tattttacaa
     3601 aattgtgcct tattgccgat aggctatctc ttgaacaccg tttaccaaga gattatgaac
     3661 ctgccaatta tcgccgagtc acaaggaata atttcagaaa aagctcgtca cgttttatga
     3721 atatggtaat ttgtgaccga gaattttgca gaaagacacc gattaagaag aaggttgtca
     3781 caacgatttt cttcatttaa aatagtagaa cagaggtgag acatgatgaa aaaaaagttg
     3841 acaaaatcca ataaaaatgt tgttttgaca gggactttag caggaatagg agaatacatc
     3901 gggattgatc cgactgttat ccgtgtgctt tatgtattcc tgagcttagt tgcttttgga
     3961 agtccaatca ttttatacat tttattagct ttgattatcc catctgcgcg ttctgcacga
     4021 acaaactatg gacataacaa tgcatattat gaaaaaaaca attatcgtga acaaactcgt
     4081 gaacaaacaa aaaaacgcaa agaagcggaa aaaatcgatg atgacgaatg gagtgacttt
     4141 taatgtcgta ctttcaacgt attttagtta atacgttaac attcatatca ttggctgttc
     4201 tttttcctgc gatggtccat gtgagcagta tttggacggc cgttcttgcc agcttcgtat
     4261 tgtctgtgtt gaatatgttt gtcaaaccga ttttgacgat actgtccctg ccctttacat
     4321 tagtcacatt gggattgttt acgtttgtta tcaatgcagc gattcttaaa atgacctcct
     4381 tttttgtggg agcagaacat tttagttttt cttcattcgg tgcagcgttg ttagtagcta
     4441 tcttgatgtc gcttgtcaat gtaattgtca gtgaacataa cagtagaaaa taaaagaaag
     4501 ctgtgccgaa ctatcggcgc agttttttta tttagaatca ggaatgcgtt ttctattaca
     4561 ttggtaatta aataaaaacg ttttttttaa ttggtagtac ttttggctag acaatgataa
     4621 aatgatagag aacactctaa tagaaatgag gtagcatgaa tgtcagaagt tgtaaaagtt
     4681 agccaattag tgaaaaaatt atctttggaa atcgtgacag gcgacgagaa aagcttagac
     4741 cgtgtcatcg taactgggga catctcacgc ccaggactag agttgactgg ttattttaat
     4801 tattattccc ataatcgttt acagctattt ggcagtaaag aaatcagttt tgcggaacgg
     4861 atgatcccag aagagcgttt gatcatcatg agaagattat gtagtgaaga tatgcccgca
     4921 tttattattt caagaggatt accggctcca gaagaaatga tccaggcagc aaatgaaaat
     4981 gggatagctg tactgcgttc tcccatctca acttctcgtt tattaggaga attatccagt
     5041 tatttagatg gtcgccttgc ccctcgaaca agtgtccacg gtgttttagt ggatgtttac
     5101 ggattaggtg tactgattca aggtgacagt gggataggaa aaagtgaaac agcgttggag
     5161 ttgatcaaac gcggtcatcg attgatcgca gacgatcgag tagatgttta tcagcaagat
     5221 gaattaacgg tgatcggaga accgcctaaa atcctggaac atctaattga aatccgagga
     5281 atcgggatca ttgatgtaat gaatcttttt ggtgcgagtg cggtacgtgg ctctatgcaa
     5341 gtccagttgg ctgtttatct ggaagcatgg gcaaaagata agaaatacga ccgtctgggc
     5401 tctgaagaca cgaccgttga aatcgcggaa gtgggcattc cacaaataaa aatccctgtc
     5461 aaaacaggac gaaacgttgc aatcatcatt gaagtagccg cgatgaattt ccgtgcacgt
     5521 acaatgggct ttgacgcaac gaaaacattt gaagaaagat taagtcgctt gattgaagaa
     5581 aactcaggca acgactagca aggagtgaca atatgttagc acaaatcaat cgagttgctt
     5641 ttgagcttgt agggattcct atctactggt atgcattgat catcgtatca ggtatcatta
     5701 tcgccatgtg gcttagcagc agagaggctg ttcgggtagg attaaaagca gatgatgtga
     5761 cagattttat gttgtgggga ttgcctatct ccattattgg tgctcgctta tattatgttt
     5821 tatttgatct tccccagtat atagctgatc caatccaaat attcaatatc cgttcaggag
     5881 gcttagctat ttatggcgga ctgattgcag gcgcgatcac gctgtatttt tttactaaat
     5941 atcattttat ttctttgtgg acttttttag atatcgctgc accaagtgta ttattagcac
     6001 aggcaatcgg tcgttgggga aatttcatga accacgaagc atatggacca gacacgacgc
     6061 gaagcttctt agaaagtctg catttaccgc aattcatcat tgataatatg tatatcgaag
     6121 ggatctatcg tcaaccaaca tttctttatg aatcactttg gagtttgatt ggatttattt
     6181 tgcttttgtt tttacgaaag aaaccacacc ttttgaaaaa aggcgaagtc cttttatgtt
     6241 atctaatgtg gtatagtttc gggcgtttct ttattgaagg attacgtaca gacagtcttt
     6301 actttttagg tgtgatccgt atttctcaat tattatctgc tgtgttattt gtcggtacat
     6361 tgattctatt tatttggcgc agaaaaaaag gaaatttacc ggattatgac cgctcattag
     6421 gaaaaaatca acaaattatc tgatttttag agcgttaaaa atgaaaaaaa tttcaggagt
     6481 ggtaaaatga attatagagt agcggtttta ggaccaggct cttggggtac tgctttggca
     6541 caagtcctcg ccgaaaacgg acatgacgtt cggatttggg gacatcgtgc ttcacaagta
     6601 gaagaaatca acacccaaca cacgaatcaa cgttatttac cagatcatta tctacccgat
     6661 tccatccaag cattcaccaa tatggaagat gcagtaaaag acgcagatgc ggtgctattc
     6721 gtcattccaa cgaaagcaat acgttctgtt gcaaaagaac tagtgcctaa attgaataca
     6781 aagccagtga tcatccatgc aagtaaagga ttagaacagg gaacacataa aaggatctct
     6841 gacatcttgt tagaggaaat cccagcagaa aaaagaaaag ctgttgttgt tctttctgga
     6901 ccaagccatg cagaagaagt agcgaaacat gacatcacta cgatcacagc tgcaagtaca
     6961 gatgcaaaag cagcaaaata tgtacaagag ctgttcatga ataattattt tcggatctat
     7021 accaatcctg atgtgatcgg tgtagagatg ggggcagctt tgaaaaatat tattgctatt
     7081 ggtgctggcg ctattcacgg cttagggttt ggcgataatg caaaggcagc aatcatgacg
     7141 cgcggattag ctgagatcag ccgtttaggt gtagcaatgg gtgccaaccc gctgaccttt
     7201 atcggattaa gcggagtagg cgatctagtc gttacttgca caagtgttca ttctaggaac
     7261 tggcgcgcag gcaacttgtt aggacaaggg cagcccctag aagaggtgct agccaatatg
     7321 ggaatggtcg ttgaaggtgt gaatactact caagcagcga tggaactagc ccaaataatg
     7381 gaaatcgaaa tgcccatcac tcaaacaatc tatgaagtgc tgtatgaaca aaaagaaatc
     7441 aaaacagccg caaaagaaat catgttacgt gatgggaaaa aagaaaatga attctcatta
     7501 acaatttttt gaaggagcga atgtatatga aagtcaaaaa agcagttatt ccagcggcag
     7561 gattaggaac aagattttta ccagcaacaa aagctatggc aaaggaaatg ttgccaattg
     7621 ttgataaacc aacaatccag ttcatcgttg aagaagcgtt gaaatcagga atcgaagaca
     7681 tcttgatcgt tacaggaaaa gctaaacgac caatcgaaga tcactttgat tcaaatattg
     7741 aattagaaac aaacctaagt gaaaaaggaa agaccgaatt attgaaatta gtagaagaaa
     7801 cgacagatgt caacttgcat ttcatccgtc aatctcatcc aaagggttta ggacatgccg
     7861 ttcttcaagc aagagccttt attggaaatg aaccgtttgt cgttatgcta ggcgatgata
     7921 tcatggaaga tgaagtacca ttgacacagc aattaatcga tgattacgaa cgtactcatg
     7981 cttctacgat cgccgttatg caagttcctc atgatgaaac ttcaaaatac ggaattatca
     8041 atccaggaga agtattggaa gatgggttat acaatgtcaa aaactttgtt gaaaaaccag
     8101 atccaagcaa agcaccaagt gatttagcta ttattggacg ttacttattg actccagaaa
     8161 tttttgatgt attgtccgag caacaaccag gagccggaaa tgaaatccaa ctaactgacg
     8221 caatcgatac attgaataaa acacaacgcg tatttgcccg taaattcaca ggtaaacgtt
     8281 acgatgtcgg tgacaaattt ggttttatga aaacaagtat tgaatatggt ctaacacatc
     8341 cagaagtagg tgcaccttta cgtgagtata tcctgtcttt aggtaaaaaa ttagaagcag
     8401 aaggaagcaa gatcgatcat aatccaaaag gaataacaga aatcaaagaa gaaaagaaaa
     8461 aataaccctt ttttctaacg agcttcagct gctaattgaa taaaaagtag ttgaagctcg
     8521 tttttatttg tttaaaaaac acgaaaatgc tatccttata gaaaagagag acaagaaggg
     8581 agtttgatca tgtcaggagg agaaattgca ggattgatcg cagcaatcgc ctttgcgata
     8641 cttgtcgttt ttatcgttcg tgtgttgttg caagtttcta aaactttaga aaaagtagat
     8701 cagactgtag cagaagcaaa tacgacgatc gaaatcgtca caaaagacgt agatcttttg
     8761 tctagacaag tagaaggctt gctggtcaaa agtaatgaac tgctcttgga tatcaatgga
     8821 aaagtcgcaa cgatcgatcc attgttcaca gcagttgctg atttaagtga aagtgtatca
     8881 gaacttaatc attctagtaa aaacatcgcg acgaaagtcg gcggaatcgg taaaacgaca
     8941 gctaaagcga cggttgccgg aaaagtcggt gggacagcga tgaaattttt taaaaacaaa
     9001 aaacatactg aaacgacagg aggaaaataa aaatggctaa aaaaggtgga tttttattag
     9061 gtgcagtagt cggaggaaca gccgcagcaa tcgccgcgtt attgttagca ccaaaatctg
     9121 gtaaagaact tcgggatgaa ttagctgccc aagcggatga tttgatggat atggcttcag
     9181 attacacaga tatggcgaaa gaaaaatcga ctgagttaac aggtattgca aaagaaaaag
     9241 cgcaagatct ttctcaacaa gcaggtgatc tagcaggtag tgtcaaacaa aaagtaagtg
     9301 aatcagcaga tgaagcagga agtgaatctg acgatatctt gttaaatctt aaagaacaat
     9361 cagctgactt atctaatcgt ttcaaaaaaa cggcagaaga tatgaaaaaa cctgcagatg
     9421 aattaggaag tattgctaaa gatacttcag aagatatcat gattgatgta aaagacgctg
     9481 ctggtaaagc aggagatact gtcaatgaag gaatgtcaga agcaaaacca gttgcagatg
     9541 acacaaaaga aatgatgaaa atggcaaaag aagatgcgaa agaagctaaa aaagaaataa
     9601 aagatacaat gacagattca tctacaaatc actaaaatca ctaaaatcac aaatttatag
     9661 aataaaaagg tgggagcgga ctagctctca ccttttttac tcttattttt ctaaaactag
     9721 taattctttt ggtgtagtcg tgaatggcac acagccatcg cttgtcacat atacacagtc
     9781 ttcgatacgt acccctactt tttcaggaat atagattcct ggttcaagag agaagcacat
     9841 cccttcttcg attactaagt catttccttc tacaagggaa gggaattcgt ggacggttgt
     9901 tccgatcccg tgacctaaac ggtgattgaa atattcgccg tagccatagc tgttgatcac
     9961 gtttcgtgcg atttgatcaa gttcacctgc tgtcacacct ggacggacgg cttcttgtgc
    10021 ggcaagctgt gcttcaagag tgatgttgta tattttttct tggaagtcgg ttggtttttg
    10081 ataagcaatt gtacgagtag catcactgca atagccattc caaacaacgc caagatcgaa
    10141 taacacaagt tcattcggtg aaatcttcgt gtttcctggt gtaccatgag ggcttgcagc
    10201 atttgctcct gcaaggacta atgtgtcgaa agacatttgt gaaacacctt gttttttcaa
    10261 ttggtattcg atttctgcaa tgatcgcttg ttcagtcacg ccttctttca ctgctttaaa
    10321 gccaatctca aatgcaacat cggcccattt accagcttcg atcagtttgt ctttttcctc
    10381 tagagttttg atcagttgta gacgttggat gatggaagct agatcagtgt aagaatccgt
    10441 tcccgacaag atatcagaga tagcatcgaa gcgctcgaca gtcaaggcgt tcttttcaat
    10501 ccctacattg ccaggctgac catagcgagc acgaagttct tttccgatga tctcccaagg
    10561 gttttcgctg tctaaatagc cacggacatc atacgcccaa ctactgtttt gagcatcttc
    10621 aacttccaaa gcaggagtga aaaggaaagg atcttgttca agagggataa aaagagctag
    10681 tacacgctca tgaggatcac tttcgtatcc ggaaaagtaa gcgatgtggc cagggtcgct
    10741 gatataagcc aattcgactt ggtgttgtgc catccattga cgtagttcat tgatttttac
    10801 gttattcatt tggcaaccta ctttctaaat taattttgta ttcaataatt gtaccactga
    10861 tttattaaaa gaccaacgtt ctaaaaactg aaaaaagtaa aaattttcat gaaaacagag
    10921 ataaaacggt tgcaattctt gagattattt gatattctat tttagaaatg taagacgtat
    10981 tttcagaaaa catataaaat atgaatagga gaacacaaat ggaaaagcaa acaatcacta
    11041 tctatgatgt agcgagagag gcaaatgttt cgatggctac tgtttcacgt gtcgtaaatg
    11101 gcaatccaaa cgtcaaacca gcaacaagaa aaaaagtctt ggaagttatc gatcgcttgg
    11161 attatcgtcc aaacgcagtt gcgcgtggct tggcaagtaa gaaaacaaca acagttgggg
    11221 taatcatccc tgatgtcagc aatatgttct ttgcttcttt agcacgtggt atcgatgatg
    11281 tggcaacaat gtacaaatac aatattatct tagcaaattc agatggtaac gaccaaaaag
    11341 aagtcaatgt attgaataac ttactggcta agcaagtgga cggcgtgatc ttcatgggtc
    11401 accatatcac agacgaaatc cgtggagaat tttcacgttc aaaaactcct gttgtattag
    11461 ctggctctat tgatccagat gaacaagtag gtagcgtaaa cattgattat gtatcagcaa
    11521 caaaagatgc agtaaacaaa ttggcaaaaa gcggcaataa aaaaatcgct tttgtcagtg
    11581 gtgcgttgat cgatcctatc aatggacaaa atcgtctgaa aggctataaa gaagcattaa
    11641 gcgaaaataa cctaccatat agcgaaggat tgatctttga agcacaatac aacttcaaag
    11701 atggtctatc tctagtcgat cggatccata atagcggtgc aacagctgtc tatgtttcag
    11761 atgatgaatt agccatcggt attttggatg gtttattaga ccgtggcgtg aaagtaccgg
    11821 aagaattcga aatcatcaca agtaacaatt cactactgac agaagtagct cgtcctcgtt
    11881 taacaagtat cacacaacca ttatacgatt taggggcggt atctatgcgc ctattgacga
    11941 aaatgatgaa taaagaagaa gtcgaagaaa aaacgattat cttgccttat ggaatcgaag
    12001 aaaaaggatc tacaaaataa taaaaggaca accattcaga ggttgtgagc ctgccgttta
    12061 actctgcgtt acaaggagca atttcaaaaa acagcatttc atgtttgatg aatattgtga
    12121 ctcgtgattc ggagttggca ggagaacacc gtttattcgg gaaaaggggt tatgacaaaa
    12181 ttttgtttaa tccttttttt ttgtcttaaa aaagagagaa agtggttgaa attacattta
    12241 tcgtatataa aacaatccgt gtatagatgc tgcattatgt tcaccaccat caaaaaaata
    12301 agggactgtg acaatactat tgtcacagtc ccttattact gtgtattcta ttaaatcaat
    12361 catctttctc atctgattct ttggttgctt gattcgctct tgcataagct gaagcggtgt
    12421 tccagtaatc tgtatagttt tcatcggtac cgccaacgcc aaatttaaag gcgcttttct
    12481 tcggtccatt tttcgcccat agcgattcaa ctgtttttcc tggtgtttgg atggattttt
    12541 tattgacagt aacttttcca ggtgtttgac cagtaaatcc agatactttt ttcttcatca
    12601 catcagatga taattcgaat ttctcatcag tgccaaaaat gtcaggtttt gcttggtaag
    12661 cttgattgat cagattggca agataattag ccgaacgtat ccttgctttg ctgtccattc
    12721 cagtattgtc atcgtgccct gcccagctac tgatggtaat agacggagta gagacaatta
    12781 gccatgaatc tcggtattca ttggtagaac cagtttttcc gacccaatct gcttttccta
    12841 gatttccgtt taagcttgag atggcatctt tgaatggtgt agtgattttt gcattgatca
    12901 cactgcgcat catgtcattc atgatcgatg ccgttgcttc cgaatagatc cggacagagt
    12961 tgctttcgtg tttataaatt acatttcctg cgttatctgt gatggaatcg atgatatagc
    13021 cttcttcata gacgccgccg tttgctaaag cctgaaatcc attggtctgt tgaagcgtcg
    13081 tgacatctac tgtacctaaa ggagcagatt caacgcccca attgtcattt gccgggtagt
    13141 tcatctttgc caagtagtgc tcgtaagcat attgtttcga tccaccggaa ttcaacacat
    13201 cttgatataa gtgataagcc gggatattgt tggaccattc tagtgactca cggacggttt
    13261 ggaaggtgtt cgatccttcg ttagtagcat tgacgatttt ctcgccagca ttttctcctt
    13321 cttgccaagt tgttgcataa tctgatacac gggattctga tccgataagc cccatgtcga
    13381 ttgcaggtcc ataaacaaga actggtttga ttgatgagcc agcttgacgt tctgtatcga
    13441 aggcatggtt attttgattc tgactgtaat tccgtccgcc aacgaatccg tagatgcggc
    13501 cagttctgtt atccatcaat acatttccgg tttctacttg actgccagca ccatcatcta
    13561 acatgtatcc ataattttct acgccatttt gcaatgcagc atagacatct ttatcgattg
    13621 tcgaatggat tgtatatcct ttattttgga tcgtttgctg tgccttttga taataagcgt
    13681 cactgaccga tgaatcagac atatcagcgt tgtctttttc agccaattgt tgtgcaatga
    13741 ttcttgttgc ttcattcatc acagtataat acaagaattc tctgtcagtc tgttctgcga
    13801 tttgttgtgg tagaaagtct tttgtaaggt cataggcttt tgcttcttcg tattgttctt
    13861 tcgtaatttg gccttcacga tacatactga ataaaacaaa atcttttctg gcaaggccgg
    13921 cagaaagatc ttcttttagc gcacctgtgt tcgtgtatgg actatacgtg atcgggcttt
    13981 ggggtaatcc agcgatataa gcagcttgcg gcagcgtcac atcttttgca tctacaccaa
    14041 agattcctct tgcagcttcc tggactccag caatgttttg tcccttatta ttacgaccaa
    14101 atggtgagac attcaaatac gtagcaatga tctcatcttt tgaaaagtat ttttctactt
    14161 gtgcagaaag aatgatctcg tttgctttac gtttgaatgt tgtttcatct gtaaggatct
    14221 gctgtttgac taattgctgt gtcaaagttg atccaccaga agagccaata ccagtagctt
    14281 cagatactaa tgctcgtaaa acagctttag gaacgtaacc ttggtgttta tcgaagtatt
    14341 catcctctgt gctgataatt gcagttttca ataaaggtga aattttatca gatgaaatag
    14401 tcgtgcgcat cagatctgtc tggatttttg atatctctgt attatctgca taaacaatct
    14461 ttgacgtttc tgtaatgttt cctagttctg tctgcagtgc ttttttagtc ggcaattgtg
    14521 tgtcttcgac gagataggca aaatagccag cgcctattcc aatacctaga gctgcgaaca
    14581 tcaaaagaag aacagtaata aaaacagtca atgattgaaa aactctaaaa ataatcttag
    14641 gaacaaacca tttttctttt tttcttctat tctgtttttt tcgagtattt ttgcgtggca
    14701 aaagggtcac ctcaactaat ggttttattt cgctaattat agcaaataat agaacaaaaa
    14761 aacagacaag gctgtttttg gcaggaattt cttttttaaa agagttaaaa cgtgagaaaa
    14821 tgatcttttt tataaaagag ttcttaagct tttctttaga gaaaatttca gttactatgg
    14881 agaacagggc aaagaaaaat caatcatttt ctttgtatat catcttacca atatatagga
    14941 attgttagta acaattgcct ttcaaggagg aatccatcca tgttattagg ctcacacgtc
    15001 agcatgagcg gaaagaaaat gctgttagga gcagcagaag aagcagccag ttatggatct
    15061 tcgactttta tgatctatac aggcgcccct caaaacacta gaagaaaagc catcgaagag
    15121 atgaatattg atgccggaaa agcgtacatg gcagaaaacg gtctaacaaa tatcgtcgtt
    15181 catgcccctt atatcattaa tttggggaat acgatcaaac cagaaatgtt tccttttgcc
    15241 gtccaatttc tgagagaaga gattgttcgt gcggaagctt taggcgctac tcaaattaca
    15301 cttcacccag gtgctcatgt aggggcagga acagaagccg gaattgctag aattattgaa
    15361 ggcttgaatg aagttttgac aaaagaacag acggcacaaa tcgctttgga gacaatggca
    15421 ggtaaaggta cggaaatcgg tcgaacattt gaggaattag cgcaaatcat cgatggagta
    15481 aaattgaatg aaaaattatc cgttactttt gacacctgtc atacgaatga tgccggttat
    15541 aatattaaag aagacttttc aggtgtaatg gaagagtttg ataagatcat cgggttagat
    15601 cggcttaaag tactccatat caatgattct aaaaatccgc aaggttcgca taaagaccgg
    15661 cacgccaata ttggttttgg aacaatcgga ttcgacacgc tgaataagat cgtccatgat
    15721 ccaatgtttg acaatatttc gaaaatcttg gaaactccct atgtcggcaa agataaaaag
    15781 aaagcttacg caccatatgg aaaagaaatc gccatgttca aagcacaaat attcgatcca
    15841 gaagtgtttc ctgagttatt agcagaagaa gtgatactct aagattttct atctgacagc
    15901 agcaaaaaag gaccaggcac attgccttgt ccttttttgt ttaagaaaca aaggagtgct
    15961 ttttgcagtc tttttcatca tgcatcttct cccccctctt tttaaattta gacaaacagg
    16021 gtatactggt agaaaagtaa aaaaacgaaa agtttcacaa gcaagtggca aaacgtgatt
    16081 ggaaggacga aaagatgctg aacactttaa aacccttgat caaccaacta aaaaagaaac
    16141 atacatactt tattcttggc atagccctat tatttacaat tattcgtttg tatttatata
    16201 gtaaagcaat ctatggaaca gacatgtcgg caacctatga tgaccagctg ctgattcgct
    16261 atgctcaaaa tattttggac ggtaaatggc taggggagta tacgactaca acactttcca
    16321 aaggaatctc ctacagctta tttttagtac ttgcacataa actgcatctt tcttatgcag
    16381 ttttgttcgg catgttcagt atttttgcca gtatattggt tgcactcgcg tttaaaccga
    16441 tcgtgaagaa caagtatctt cttttaagtt tctattttta ttttttgttt tctccgatta
    16501 gttttacaca tgaatattcc actcgaacgt atcgtaatgc cctcgtgatt ccggctgttg
    16561 agatcattat cgcttgttta ctagctattt attaccgtaa atatagttcc agcaaaaaaa
    16621 tgctgccgtg gtttttaggg ctagctgttt cttttccttt cttttggttc attcgcgaag
    16681 attccatctg gcttcttcct tttactgtag tcgcgttagg catcagtttc gtccagatct
    16741 tgctaggaca agacttttct ttcaaagaag caaagaagtt aaaagaacga ccatttctcc
    16801 tttcgaaaca agaatggctg aaactttcat tgtttttact ccctctagtc ggtttctcca
    16861 tcgtctatca gtggatcgaa tataaaaacg agacaacata tagtattgcc gccatcaatg
    16921 accgttcgag tggcgaattt ggacgtttga tgaaacagct gatccgaata gatgacgaaa
    16981 cgaacttaaa taaagaaaac agtgagatat gggtctcgca tgctgcatta aataaagcat
    17041 tagacgcatc tcctacattt catgctgttt ctactcgtat tgacgaattg tatacgactc
    17101 atgcgtggac ccaaggtgga aaagtaaaag aattaccagg agatatcatt ttttgggcat
    17161 tgcgagatgt ggtaagcgaa tctggttatt accaaaacaa tgcaaaagaa accaatgatt
    17221 tttggaaaaa agtcaatcaa gaattagaag aagcctatca aaagggaact atcaaaaaga
    17281 aaaaagaatt ctatctgatg ggatcagggg atggaaagac gatgaatgat ctgccaatcg
    17341 tatttgcttt tctgaaatca ggatatgcct atgcatggcg atacaaagat ttccatcaag
    17401 gggggaatta tagtttcggt acgcaagaac aagtcgaaca ggcccaagaa ttattgaatc
    17461 ttccactaat ggataattgg atcgatagca gtgatccgag aacacctgat ttccagttga
    17521 caaaaacagc aaaagtcgcg aatgtcatga taaaaattta ccaagttctg gccattcccg
    17581 tgctgattgc ttcaatagca gggatattac ttatgatact aggaaccgca tttgcaaaag
    17641 ctgatcgtgc ctattatagt tcctctttga ttgtagtcac cggattaata ctgacacaat
    17701 tggtttttct tttcggcgtc tcgtggtttt gttcttatgc gccagaaaaa aaggattatt
    17761 ttttaagtgt ctacactggc gcaggcgtgc cattaacgca attcatcgta gtaacaagca
    17821 tgctagtttt cagtaaattg aatctgaaaa aagctttatc ttccaaataa aaacatacta
    17881 agattagagg agaacatcat tgacgaatga tgttctcctc tattttctat ttttagatta
    17941 tagtgaagag tgaaagaaag ttcgaacaaa gccttgagga ctatgagtcc ctatcaaaaa
    18001 ccgaacttct ttactgtcat tttagaatca ggtttacgta tgttggggcc atgagcccgt
    18061 gtataggaaa ttggaatcga aatgaagcag taaagttctt tcaaaactta aaaaggagga
    18121 aaacaaatgc cttacattct tgcattagat gtttcaatgg gacacagcta cagtgtccta
    18181 tacaaagacg acacctgctt atttgaagac gagatcgaac acactcaaag aggatttcat
    18241 gcattactcg aagagatcca gcacttgcct gaacgtccat tgattgtttt tgaagcaacg
    18301 gggatttatt ccagaccagt cgaaaagttc tttcaagatc atcacttttc ttactgtttg
    18361 ttaaacccgt tagaagcgaa aaaacagatg gaagaaaaca ccttatgcag ttggaaaaca
    18421 gataaatcgg atgcccatcg gctcgcacag actcatctta aaaatcaacg tcagccaaaa
    18481 gaagtacaaa gcaattttta cttagagatg cgcgatttag cccgttttta tcaagaaatt
    18541 gagaagaaaa tcactcgtct gcgcatggat ttacacaact gccttcagtt gacttttcca
    18601 gagcttgaac agttcttttt aaacaggctg accccttacg cattgatatt gatccgtctt
    18661 tttccccatc cagattttgt attggcatct actcggacaa aaataaaaaa taaactgatc
    18721 aacgaaacac ggaaaaaaat ctccgcgaat cgtgccgagc aaaaggcaga tcaaatcatc
    18781 cactatgcac agtgtgccta tccagctgtt gaaaaagaca gtatccactg tcaaaaaacg
    18841 atctattacg ctgaacttct ccaagattta ctcgaacaaa aagaagcact ggcgacacaa
    18901 atgatcaaaa aagccgaagg ctctccttgc tttttgttgt accaaacgtt ccctgggatt
    18961 ggcgcactca cagccgcttt attgctcggt gaattaggag atatcacacg atttaaaacc
    19021 cataaacaat tgaatgcctt tgtcggaatc gacatccgac ggtatcattc aggtaagtat
    19081 actggccaag accgcatcaa taagcgtggt aatcccaaag cacggaaaat tattttcttc
    19141 accattcgaa atatgattcg ccagcaacgc gcagctccta atcatattgt cgattattac
    19201 tacaaattaa aaaagcaacc tatccctaag aaagataagg ttgccacagt tgcttgtatg
    19261 aacaagcttc tgaaatgtat gcacgctatg gttagggcac atacagagta cgactatgcg
    19321 tacgcggtct cagtggacca ttaactaaac cacttacagt ataccacttc tttgcccaga
    19381 tcgaaaattt tttacgaacg gggctttatt tggtgtgcct tgattttgtc gctttcgttt
    19441 atgagctatt tttcttacac cttttacttg actaatcgta ggaaagggaa ctgtgacaaa
    19501 agtcttgtcg cagttccttt ttccgaataa acggtgctca aaagcttgca tctgcggtaa
    19561 taagtccaac caagttaaaa acataa
//
LOCUS       DBNICI010000040        19408 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.040, whole genome shotgun sequence.
ACCESSION   DBNICI010000040 DBNICI010000000
VERSION     DBNICI010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.040"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..1340)
                     /locus_tag="AC1ZCC_001161"
     CDS             complement(<1..1340)
                     /locus_tag="AC1ZCC_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1629426.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIK"
     gene            1488..2656
                     /locus_tag="AC1ZCC_001162"
                     /pseudo
     CDS             1488..2656
                     /locus_tag="AC1ZCC_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(2782..3861)
                     /locus_tag="AC1ZCC_001163"
     CDS             complement(2782..3861)
                     /locus_tag="AC1ZCC_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIQ1629427.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(3964..4404)
                     /locus_tag="AC1ZCC_001164"
     CDS             complement(3964..4404)
                     /locus_tag="AC1ZCC_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIQ1629428.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(4461..4871)
                     /locus_tag="AC1ZCC_001165"
     CDS             complement(4461..4871)
                     /locus_tag="AC1ZCC_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HIQ1629429.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(5051..5485)
                     /locus_tag="AC1ZCC_001166"
     CDS             complement(5051..5485)
                     /locus_tag="AC1ZCC_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIQ1629430.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(5585..6433)
                     /locus_tag="AC1ZCC_001167"
     CDS             complement(5585..6433)
                     /locus_tag="AC1ZCC_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIQ1629431.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            6822..8588
                     /locus_tag="AC1ZCC_001168"
     CDS             6822..8588
                     /locus_tag="AC1ZCC_001168"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HIQ1629432.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            8690..9949
                     /locus_tag="AC1ZCC_001169"
     CDS             8690..9949
                     /locus_tag="AC1ZCC_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIQ1629433.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            10096..11397
                     /locus_tag="AC1ZCC_001170"
     CDS             10096..11397
                     /locus_tag="AC1ZCC_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1629434.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            11400..12251
                     /locus_tag="AC1ZCC_001171"
     CDS             11400..12251
                     /locus_tag="AC1ZCC_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HIQ1629435.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            12264..13079
                     /locus_tag="AC1ZCC_001172"
     CDS             12264..13079
                     /locus_tag="AC1ZCC_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HIQ1629436.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            13230..14396
                     /locus_tag="AC1ZCC_001173"
     CDS             13230..14396
                     /locus_tag="AC1ZCC_001173"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIQ1629437.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            14626..15273
                     /locus_tag="AC1ZCC_001174"
     CDS             14626..15273
                     /locus_tag="AC1ZCC_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIQ1629438.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            15357..16301
                     /locus_tag="AC1ZCC_001175"
     CDS             15357..16301
                     /locus_tag="AC1ZCC_001175"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HIQ1629439.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            16436..17887
                     /locus_tag="AC1ZCC_001176"
     CDS             16436..17887
                     /locus_tag="AC1ZCC_001176"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIQ1629440.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            17908..18744
                     /locus_tag="AC1ZCC_001177"
     CDS             17908..18744
                     /locus_tag="AC1ZCC_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIQ1629441.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            18744..19403
                     /locus_tag="AC1ZCC_001178"
     CDS             18744..19403
                     /locus_tag="AC1ZCC_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIQ1629442.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
BASE COUNT         6533 a         3442 c         3710 g         5723 t
ORIGIN      
        1 attttgatcg attcatttga aaaaatttgt tctatcttaa cgatcagatc tttactgata
       61 taaaaatcac gcgtatagtt cacaccaatc acaattcgcg gctgtttttt gtagcgatca
      121 tacaaatgta ctaggtcttc aaccatccat tgcacatttt ccttttctaa aataactggt
      181 aacaagtgtt gattttgttc tctgaaatcc atataaaatt gttcaatggc ggtttggact
      241 tctttcaaca cgctatgttc ttgataaagc atgaaagtag gaaggggcgt atttggttga
      301 ttgaagttaa gaaatccatt ataaagttta tttcgtgcga atgccaatga gtaaagaatc
      361 ttttcttttt caggtataaa caaatcaaat acagtacaaa aggactccac catttctctt
      421 gaataaagaa catgaggaac gtttgctttt tttaaaagtt tgtattgctc caagcttgtt
      481 tcttccgatt cgattttcgt gatagaaagt cgagctaaaa tattcactaa gatgcgttgt
      541 tcgcgacaaa ttggcagatt aggtcgcaaa atatctacag agggatcaat aaaaaagtca
      601 atatattcat actctgtgaa ttctttttgt acgtctttca cagaagttgc aggaaaattc
      661 tctttacata tttttaatag aaaatataat ttatctatca taccgctatt tagtcggtca
      721 agtacttctt ttttaaaaaa agtattcatc ataaaagcca ctacttgcgg atcttcatca
      781 taaaattctg tagcagtact tgaaaagatg ttccaataaa tatctaatgc tgcatattga
      841 atttgtacct cttttccaga aaaagacttt ctgccattat tagaaaagga gatagacaaa
      901 ccgtacaatg ctaaaaatgc attaatcttt ttcattttcg tatacaaata agaggcacta
      961 tagtttgttt gctgacttaa actgataatc gaatttgtct gacagctgaa caaagaagtc
     1021 aagatctgat aactgactga ttcgttgcag tatcttccaa atagacgata atagcagtaa
     1081 gccggatcaa ttccttcaaa attcaacttt aaaaaaggtt tttgcttatc aatatgcagt
     1141 tctgttagat tgagacgctc aaagtcttgt tctaattctt tgatgtatag taaaaaattt
     1201 gattcagaca tatggaaatg atctgctaaa tattgtgtag taataaagaa acgatgttca
     1261 gataggaaaa gaaaaatatc taattttgta ttgctttttt tcttcaagag aaacgtttta
     1321 atcgcttctt gagtttgcat gaaagaggct cctttttact taaattgtta aaacttgaaa
     1381 atattactgt ttttttcata aatttagaca aaaaaacagt aaacatatag gtagaatctc
     1441 tactatctta tttcttgcat acaaaaaaat caaggagtga gagagttatg agatattcaa
     1501 ccatcttaat atcaacattt gcagccttaa gcttatcgct tactataaat cagacagtcc
     1561 acacagagga gatagaaaca gccaaatgga cagaaaccaa gtgggccaac gtcaatcgaa
     1621 ttacctttca ggatgaattt tcatccttca attcaggagt cgcaaaatta agaaaaggac
     1681 aaacagcgac catcaaagca aaaattgact attcaggaga cttagcacct atactacaat
     1741 cagctgttac atttgagaat gttgatcccg ctttgtcgat tggctatgac gagaacactt
     1801 tgactatcca acacaagcaa gcgatattta ccttcaccgt tacattaaat caggacttgt
     1861 taaaaccagc gctttttact gtcaaggtat cagatggact accgaacgat catcatcatt
     1921 taacgccgta tagtcagcgg cagacaattg agcaagattc agctatcgac tcaggaggag
     1981 atcttgttga agaaccaaca gataaaccag aaaatgaaaa taagccagaa gtaccgccta
     2041 cagaaaatcc agacggagaa cagaaaccgg aaatagaacc tggagaagaa cccgatacag
     2101 aaactcaacc agagccagac aatgaatcaa aacctgaaat aacccctgga gaaaaaccag
     2161 atgtagaccc agaagaaaaa ccagatgtga ctccagaacc agatacggat tctggaaacc
     2221 aaacagtacc ggaaacaaat ccagacacag ataatgaaac tgaaaatcca gaaaaaccag
     2281 aggtagatcc agaagaaaaa ccagatgtga ctccagaacc agatacggac gcaagagacc
     2341 aaggaatacc agaaaaaata aacaaaaaaa caattcaaga ggatggaaaa aaagagagta
     2401 aaaaatcaaa tctagcaatt ctaaaaataa atgaagagca gctgaataaa aaatcacgaa
     2461 tatttgatag cgcccaatca gctgaaacct taaaaagtag caaggataca acctttgctg
     2521 tcctgaaacg aaaaataaac agctaccaaa atccggagag tcacaaaaca aagttatact
     2581 gtggagcgga atcattctat taagtatagc aacaatgctt tctgcaaaaa gatttaaaca
     2641 aaacagatct ctctaacatt tgaataatgt ctgtgaacgc atttttcaaa taaaaagaaa
     2701 tatccagaaa atgaccccaa aataccagat tgaaatctga tatttggggt cgttcatact
     2761 ttatctttct tcatatttat tttaagggat atctgtttct tttggaagat ggttcgggat
     2821 ctttttcact ttcaatacat ctaaaaagaa ggtaaagatc ggaatgccga caatcaagcc
     2881 ccaaacgcca aagaaccgtt cgctaacaag taagaccaca aatgtataaa aaatcggcag
     2941 ttctgttttg ctggacatca acttcggatt cagcacataa gactcaatta gatggacaat
     3001 caaaatcgta atcaggatat aaacaacatc tcggatgcct ccttgggaat aagctatgaa
     3061 agatagagga atacaagaaa tgataacacc tgctactggg atcagactta aaatgaaaat
     3121 catgatcgct aaactcagca gctgatgaaa gccaaataca gccaaggcga ttgtagtgag
     3181 caacgtattt aaaagagcga caacaaattg agcttctaat accaaaccaa atgtattgac
     3241 aaacttatcg gcaaaatagt aaatatcttg aaagaaccaa gaaaattcac tttttaaaaa
     3301 caatcttgag aatacaattg tttttttctt ttcaatcata aaaaagaaac ttaagatgaa
     3361 tgacatagca aacgccactg ccatttttcc tatatcctgt aaatagccaa gcgcaatcga
     3421 agcgccattt tgaagctgtt caaaaaaatc gtttcgctca atataagtat ggatatattg
     3481 taaaacagga tctgcatctt ttggctgatg ctcgtaaaaa ttaataacgg actggatcat
     3541 gtcaaatgtt tgatgaataa gcatcgggat atactttgtg aatgccagat agataaggta
     3601 aacgatcagt gtataaatca tgatagctaa aacacctgta gggattcgga caaatcgctg
     3661 aatgatttga acaagatgaa tcatcaaata agtaaagata aatgtgagca aaatggttgt
     3721 aatcatactt ctaactaaga aaagaacaaa aacgacaaat aataaaacag tgagacggcg
     3781 tattttttca ttcgccaaaa aacgctgata aacggtcaaa tcgacaacct ccaaataaca
     3841 aaaaataaac acgttattca ttatatgatg gaatatgaaa ataagaaaga gcaaaaagaa
     3901 aaaccgctaa atgacgttat cttatgagtt ggttttcctc ataaaatcgt tcatctagca
     3961 gtttcagccc tttttctggt cttgatgaaa attatctagc atttttcggg tcagatgttg
     4021 cagtgtatcc aattcttcct gttcaagtgg aagataagac ttgatctttt gtggaatacc
     4081 ttctgcttgt ttttgcaacg cagcaccttg agcagtcagc gtaacattta cgattctttc
     4141 atcacccgtt tcacgctgac gattaatata atttttttct gctaactttt ttagtaaggg
     4201 agtcagcgta ccagaatcca gacacaaatc atttcctagc tctttcactg aagtcttttt
     4261 cttttcccat aaaactaaca tcacaagata ctgggtatag gtaagattca gcggcttgag
     4321 gaggggagtg tagtgtctaa tgatttcttt agaggcggca tataaaggaa aacatacttg
     4381 attaactaaa tatagatctt ccatttttcc acctccgctg ttttactata gtgtatcata
     4441 gcggatttac acaaaaaagt ctattcacta actgctttca gagtaacttc tatatttcca
     4501 gctgtagctt ttgaatacgg acacacttta tgcgctgcct ctaataattc ttgcgtttct
     4561 tccagagaaa gaccttcgat agatccttca atctctacac ctaagacaaa gctcgtttca
     4621 ctttctgaat aaagggaaac ttgtgcactg acagtagaag ccccagagat atttttttgc
     4681 tgtttgatca attctaatgc actgttaaaa catgcactga aaccagctgc aaacaattgt
     4741 tcagggttgg ttgcattttc gacgcgttta ccaggagctg caatattcaa ttcgaatgta
     4801 cgatcgggat tatgcacttc tccagagcgt ccgcctgtgt tgatcatttt cgtagaatac
     4861 atttttttca taaaattacc acctttaatt atattttata caattgaatt ttacacaata
     4921 taattgtgga aagcaaagaa atggattggc ataagataaa taagagaaag cacatgaaac
     4981 agacgcgctt tctcgaatag caaacatata caaaaaatat cagagacaaa aaataatctc
     5041 atcataaaca ttacatatat ttctcaaaaa gttgttttct ttcttcaaaa attacttcac
     5101 gatggacctg atgaagcatc cataaataac caaaaggatc tctgaacatt gagttagtca
     5161 caccaaagtc catcatttct gtcatttctt gaatctctac acagccattt cgtaatgctt
     5221 tattatgtgt gtcctcgata tcagagacga tgatattgaa ccagatcgta tttggatgtt
     5281 cagcagtagg agcagtcaaa ccaagagacg tgttttcatc cagcatatgg aatctcgtac
     5341 catacaaggt aaaaatgact tcattattgc ctctagggta atctgttgcc tcaatcagtt
     5401 gtatttcatc aaagattgtt tgatacagtt ctagtgcttt caaactatca tttaatacaa
     5461 aatcgatttc cgttccttta tgcataaaga tctcctctcc ttttcagtcg aacagttact
     5521 agattctttt attataccag ctaaagaaaa cgcattcaaa aaaatagcgg atttacttac
     5581 caactcattc gattggatga taagtaaatc cgcgtcccat tacttctgct acgctgctga
     5641 caatgacaaa ggcacttgga tcgatttctt ctatcaatgg tttcattcgg gaaaattcat
     5701 gatcagtcac aacgatcaat aaaacctctt ttgactttcg attatacccg ccttttgcct
     5761 ctagcagggt aaggccacgt ccaatctcag cacttaagcg aaggcggatg tcttccaaat
     5821 aatgttcact catgaccata acagatttct ttcgattgat acctgtttcg atataagtca
     5881 tgacaattga agtgattgca atagataaaa tagcataaag aaactcttct attccaaaaa
     5941 taaacagatt aagagaaaca acgaatgcgt ctgtaaaaaa taagccaatc gaagtattca
     6001 agtggaaata ttttttaaaa atgaggggag ggatcgtcgt accgccgcta gaagagttgt
     6061 ttttatataa aatcgcaact cctaaagcaa aaatcgcact tccaaaaatg acagacaaca
     6121 accgatctga agtaatcatt gtttctggaa ctattgcaat gattaaagga aaaaccaagc
     6181 ttccgaaaag tactttaaaa aagggttttt tacctaaaaa tagaagagct agtaccaaca
     6241 taacgatatt caacactaaa ataactatag ctcggtcaaa gcctaaagca gcttccagta
     6301 atattcctag accgctgaca cctccagcag caatatgatg aggccctaaa aacatattga
     6361 tagcgactcc taatatgaag agggcaaaaa taatcttaat gtatgtccat ataaattgtc
     6421 tcgtttcttt catttattca cttccttaat tcattaataa tactcttatc ttattataac
     6481 ttacttttca ccttaagtta actttaatta ataaaaaaat taggctagtc taaattatta
     6541 tgtttcattc ttctagaata tacttctacg ccaaaaactg ttaaaaaact tttactttag
     6601 aaaattggag aaattgatcg cttaagataa attcaggaca ctaaatagta caggaagtag
     6661 actaaaactt ttaaaagttt taagtctatc tatttggcaa gtttaaaaaa taaaaattcg
     6721 gtatcaaaaa actgttaccg ataacatttt tgaaagcgtt gtcttttgct tctctatcac
     6781 ttatagtgat gacgtcatat taaaacacaa ggaggaaatc aatggataca gctgcaattt
     6841 atcatcgacc ggaaagtgaa tatgcatttc tatatacgaa tacaagattt cgtattcggc
     6901 ttcgtactag gaaaaatgat atcaaaaaag tatatgtgct atgtggagat ccatatacca
     6961 taacaactga aaaatggtat caaaaacaac gaccaatgaa aaaatgtcta agtaccaatg
     7021 ttcatgatta ttgggaaatt gaagtgacga gcgaaacaag tcgcttacag tatgcgttcc
     7081 atgttgttgg ggaagataac acggactgtt tttacggtga tcaaggaatt tttccttatc
     7141 gagaaaatgt gattactgaa ccaaattttt attttcgaat accttatttt catcaaatcg
     7201 atcgattcac tattccagaa tgggtaaaag aaacagtatg gtatcaaatt tttcccgaaa
     7261 gatttgcaaa cggggacaaa gcaaatgacc ccgaacacac tttaccttgg ggaagtaaag
     7321 atccagaccg agaagacttt ttcgggggag atttgcaagg tgtgatggat catttggatt
     7381 acttgacgga ccttggcatc aatggcatct atttttgtcc catatttaaa gccacatcca
     7441 atcataaata cgatacgatt gattattatg aggtggatcc cgcttttggc gataaaaaac
     7501 ttttaaaaaa cttgattgat gaagcacata aaagagggat acgtatcatg ttcgatgctg
     7561 tattcaatca tatgggtgtc cattcaccac agtggcaaga cgttctcaaa aacggagaaa
     7621 aatcgatcta taaagactgg ttccacatcc gttttttccc agtagattct tatcagatga
     7681 ctgacttacc agaaactgct gaaaatatcc catatgatac ttttgctttt actccattca
     7741 tgccgaaact aaatacagca aatccagaag tgcaaaaata tttgctggat atcgcaactt
     7801 attggataaa agaattcgat attgatggat ggcgtcttga tgttgctaat gaagtcgatc
     7861 atcatttttg gaaaaaattc agagaagcag ttacagaaat caaaccagac atctatatcc
     7921 ttggagagat ttggcattcc tctcaagctt ggctgcaagg ggatgaattc catgcagtga
     7981 tgaattatgc atttacagat tcgattaaag attattttgc aaaaaagaaa atcacagctt
     8041 cacaaatggt cagtggaatg aatcatcagc aaatgcttta cagagatcag gtgaacgaag
     8101 gaacattcaa cttattagat tctcatgata ctgccaggat cttgaccctt tgccaaggta
     8161 acaaagaatt gatgaagtct gtgatggcct ttatgtttct tcaaaaaggg tctccttgta
     8221 tctattacgg gacagaaata gggatgactg gtgaagatga cccggattgc cgcaaatgta
     8281 tgatatggga gaaggaggaa caagatctag agctattcgg ttttataaaa gaacttgttt
     8341 ctctaaggaa acagttgtct aagattattt ctgaaggttc tactcagtgg ctaatagtaa
     8401 atgatcgtga agataagctc tatttcacac ggaaactaga gggacagatc atctatgttt
     8461 actttaatca gtcaaaagaa ccttgggtag tagaacagga aaatgaggtg attcttagtc
     8521 aaaattgtca gttgttagaa gacgggaaag cagagatacg acaatacgga tttttgatca
     8581 tgtgttgaaa actgttaccg gtaacaacaa gaaagcgttt gcaaatgaag atttgtgccc
     8641 attataataa gggtgtaaca gcacgttgaa tacaaaggag gaaatgaaga tgaaaaaaag
     8701 aagtgtgaaa cagttgggtt tagggttaat ctctttagga atcttaggcg gattagcggc
     8761 ttgtggcaat ggtaactctt cagcagaagg ggaagccgat gaaggaaaaa cgttaacagt
     8821 ttccgttgat tctggatata aagattatat aaacgaaata aaaaacactt tcgaaaaaga
     8881 aaacgatgtg aaaatcaagc tagtagaaaa ggacatgttc gatcagttgg aatcattagc
     8941 gttagatggg cctgctggaa agggacctga cgttatgatg gcagcatatg accggatcgg
     9001 tgcgttagga cagcaaggac atctggcgga agtaaaactg ggaaatgaag cagcttacga
     9061 tgaaacagac aaagcccaag tgacttatga tggaaaaatc tacggagaac ctgcagtaat
     9121 cgagaccctt gttttgtact ataacaaaga tttagtcgat acagcaccat caacgtttaa
     9181 agatttggag aatctatcca aagacagtcg ttttgctttt gagtcagaag caggaaaaaa
     9241 tacaggattt ttagctaaat ggactgactt ttattattcc tatggtttaa ttgcaggtta
     9301 cggtggctat gtatttggtg atgatggcac aaatccttca gatatcggtt tgaataatgc
     9361 aggtgctgta gaaggaatct cttatgcaac agattggttc aaaaatgttt ggccaaaggg
     9421 aatgcaagac ataaaaagtg caggagactt cgccagccag caattcatgt ccaataaaac
     9481 tgcagcaatc attgacggtc catggcaagc gcaaacatac aaagaaaaca atatcaatta
     9541 tggtgtagcc aaaattccta ctctaaataa tggacagcct tatcaaccgt ttggcggagg
     9601 aaaaggatgg gtagtcagca attatgctaa aaacaaagac ctctctcaaa aatggcttga
     9661 ttatgtaacg aatcaggaaa atcaagaaaa attctttgaa atggtaaatg aaatccctgc
     9721 aaaccaacaa gctagggaaa cagcaaaggc taaaaacgat gaattaacaa cagctgtgat
     9781 tgaacaatat gaaacagctc aagcgatgcc aaacatccca gaaatgggcg aagtttggac
     9841 aggtgcagaa aacttgatgt tcgatgcagc atcaggttca aaaacaccaa aagaatccgc
     9901 agatgaagca gtaaaaacaa tcagtgaagc catagaacaa aaatactgat taaaatgata
     9961 accgtccatt tttataaaaa atggaacagg tgcttcagat aaaagtggca agagcgagat
    10021 ggaaaattat cttgctcttg ttgccattat cttaaaagca gtttgcggtt tagcaagttt
    10081 agagggggca ttcagatgca gcaagataaa acaagcaata tcaacgaaaa aaaaatcacg
    10141 ctttgttcac ttattccagg gatggggcag tttgtaaatg gccaagcttt taaaggtatc
    10201 gtttttttcg ccatattcgt cctattcatt ggtcagatgc ttctgggcgg atttcatgca
    10261 ttggtaggat tggttacttt gggaagtata ccaatggaag atcattcatt atttattttg
    10321 attgaaggca ctttgcaatt catcattaca atcattttct tgatttttta cggtttgaat
    10381 cttttagacg cttatcgagt agcgaagatg cgtcaagagg gcaaaaaaat caatcggacg
    10441 atgaaggaag tcatacataa tataggtgat gaaggttttc cttatctatt gacacttccc
    10501 gcttatcttc tcatgatttt tacgatcatt ttcccagtcc tagttacatt gttcactgca
    10561 tttactaatt atgacttcaa tcatattcca ccagccagtt taattgattg ggtaggggct
    10621 aaaaactttt ttagtatctt ctttttaagc tcatatcgta atactttttt tgcagtcttt
    10681 tcgtggaccg tgatttggac tttatgtgca acaactttgc aaatcaccct tggtattctc
    10741 acagcggttg ttgctcacca gaaatttatt aaattcaaac gtattttcgg tatcattttc
    10801 ttattaccat gggcggtccc agcttttatt actatcatga gtttttcgaa tatattcaac
    10861 gatagcgtag gtgccatcaa tactcaagtg attcctttca taaaccagct tccttttgta
    10921 gatatcagtg caatctcttg gaaaacggac ccgaactgga cgaaagtagc aattatctta
    10981 gtgcagggct ggttaggatt tccctatatc tatgtgatgg ttacaggaat cttacaatcg
    11041 atttcagaag acttatatga agcagcaaaa atcgacggag caaatgcatg gcagcgcttt
    11101 tcaaacatca cgcttcctgc tgtcttccta gtggcagcgc caacatttat tacacagtat
    11161 acggggaatt tcaataattt ctccatgatc tatctgttta acaatggcgg tcccggaagc
    11221 gtaggcggag gagctggttc tactgatatt cttatttcct ggatctataa acttacgact
    11281 ggaacttctc cgcagtattc tatggcttca gcaatcacgt tgattatctc cgtcatcgtt
    11341 atctgtgtgt cattgctggt atttaaaaag acaaatgcgt tcaatatggg ggattaacaa
    11401 tgaaaaaatt tcattcgagt gctcgattcc aaaaacgact ttctcacttt tttacttact
    11461 tttttctttt aatactgtca gtcattatca tctatccgct tttgatcaca gcaagtactg
    11521 ccttcaagtc aggaaatatt gcagctttta gtttagatct ttcaagtgac tggactttag
    11581 gaaactttac tcggctattc agtgaaacac tttacgggac ttggtataaa aatacattga
    11641 tcatttctgt cctaacaatg atcgtacaag tgactgtagt aactttggcg ggctatactt
    11701 atagtcgcta tcgttttatc ggccgcaaat caagtttgat gttcttttta gtgattcaga
    11761 tggtaccgac tatggcagca ttgactgcct tttatgtcat ggctttctta ttagaagcgc
    11821 tcgataagta ctggttcttg acaatgatct atataggcgg tggaattcca atgaatactt
    11881 ggctgatgaa aggatacttt gacactgtac caatagattt agatgaatct gctaaacttg
    11941 atggggcagg gcatttcagg atttttttcc agattgtatt accgcttgtc aaaccgatgc
    12001 tcgccgttca agcattatgg gcttttatgg gaccttttgg ggatttcatg ttagctaagt
    12061 tcctgcttcg aacacctgaa aatcagactg ttgctgtcgg cttacaaaca tttatttctg
    12121 atgtgacaaa ccaaagagtc tctctttttg ctgctggggc aattttgatt gcactaccga
    12181 tttgtctatt attcttcttt ttacaaaaga actttgttac cggcctgaca tctggcggaa
    12241 caaaaggata ggagcagagt atcatgcaaa catttccgtt aaactatttt tccgcattac
    12301 gtacgccaac tcaagtgttt gcgggacgca aactattatc atggcctaaa tttttcttga
    12361 tatttgtatt tttagtttcc ttgatggtca tgcctgtgac tcttttttat gccaatcaga
    12421 tccaagcgat tccattagaa caatttctat ccgttcattc gctgatcgat gaacaaggaa
    12481 cccaaaaatt ttctgaactt gaattatctg aaactgggtt acaagcatcg caacaaacga
    12541 tagctgtgac ccctgaaatt ttggtaggtg tgtctttatc tgagaaacaa caatccgatc
    12601 atggaacttt tattgatttt gagaaggaac aatgggtgat ccaacaaaag gataaatctg
    12661 gtatacgccg ctatacgatg aattatagcc catctttcca accagattct gtacgtacgc
    12721 ccgaagattt ccaacgattt ttagaaagag aattttatgc cagtaatcgt ccaacaatca
    12781 ttttatctta tagtttaagt cttggtcttg tcctatttgt catgacgagt ctcattttat
    12841 tcggggcttc ctttttcttg tggatgacaa gaaaaagcca actttcatcc attcatacat
    12901 tcaaagagag tgcaaatttg atgctgaatg ttatgggaat aggttccatg gcagcagcag
    12961 ttgtagggtt tatccacttt gactttatct tgatgctaag tgtccaaaca acgataactg
    13021 tcttgctgct tttatgggtt tttgtcaaaa caagattcaa agataagcgt gtcgaatgac
    13081 aagtttaagc gatttatgcg acaatgaatg tgaaaaaaat gaaaaataaa gtagtttttg
    13141 aaaaatttct gaaaaaatta aacttcttag ttgattcttt tcttttttag tattaacgta
    13201 atagggtagt ttttttaagg aggaaaatca tgttagagaa aactttatac aatcaattat
    13261 tttcacgttc tttctcgctc cctgtcgaag taaattattg ggacggtaca acgaaacaat
    13321 acggagatac ggacactcct ccccaaatca aaatcaatat ccatgaagaa atcccaatga
    13381 aagagattac aagtaacgct tctttggcac taggagaagc ctatatggat catcgaatcg
    13441 agatcgaggg aagtatccaa gcattgatca acgatgctta tcagcaagca aacagctttt
    13501 tacgaggaaa agattatcta aaatggctgc caaagaaaga aaaacatacg aaacaaaaaa
    13561 acaaagaaga tatccatgcg cattatgact taggtaacga cttttataaa ctttggctag
    13621 atccaacttt gacttattct tgtgcttatt tcaatacacc tgaagatacg ttggaacaag
    13681 cacaaatcaa taaagtccat catatcttag acaaactctt tatcaaagaa ggcgaaacgt
    13741 tgcttgatat cggttgtggc tggggaacat tgatgttcac tgctaccaaa gagtataatg
    13801 tcaaggcaac tgggatcaca ctaagtgaag aacaatacga tttgattacc aaaagaataa
    13861 aagaagaaca tttagaagat aaatgccgtg tattattaat ggattaccgc gaactaaaag
    13921 gcgaaacgtt tgatcatatc acaagtgtag gtatgttcga acatgtaggt tctgaaaatc
    13981 ttgaaggtta tttcaaagtg gtaaaagatt tattgaaacc aaaaggaaca gctttgatcc
    14041 atggaatcag ccgacaacaa ggcggtgcga caaatgcttg gatcaataaa tatattttcc
    14101 ctggcggtta tgtccctggt gttactgaat tagttgataa tattacaaaa aacgatttgc
    14161 aactgattga tttggaaagc ttacgtcgtg actaccaatt gacattagaa cattggacaa
    14221 agaattttca tcaagtccaa gatcaagtca cgaaagaaat gggtgattcc ttctatcgaa
    14281 tgtgggatct atatttacaa gcttgtgcag cttcatttga atcaagtaat atcgatgtga
    14341 ttcaatattt gcttgttcaa cctggaaaca atgacatccc aatgcatcgt gcataattga
    14401 ggcaaaagta ctgtttaaag actatttaaa aagaagctaa gttttatgat gctccagaag
    14461 tgaacttttt tgtctaactt ctaggattgt ctctaaagct tagtttcttt ttctttatat
    14521 tttcataata aaagtttggt tctttaattt ttaaacatga cagaaaattt tttcttcgtt
    14581 atacttgtaa tagaatacta ttttataaca aaggggatcg aatcaatgaa aaaaactgtt
    14641 atttatacta gtttatttgc agctttgttt ttagctgctt gtcaatcaaa taccaatcaa
    14701 ggcacatctg ggaatacgtc ttccagtagc gaacagcgct cttcacaggt atcggactcc
    14761 tcaacaaaag caagcacatc ttcgtctagc cagacaaaaa gttctagtga agcaagtact
    14821 cagtcagata tcaaagtgtc tgcagctcgt gcgatcgaat tgttccaaga taaataccca
    14881 gatactgcaa ttactagttt ggaattagat agtgattggg gatcttactt ctacaagata
    14941 gaaggtgtcg atgatcaaaa agaatacgaa gcaagaattg atgctcaaaa tgaaaaaatg
    15001 gagacagaga atccagaaac tctagatcga gatgaacaaa atggtcaaaa gaaaaaagaa
    15061 gatggtctgg atgttgataa attgatcacg attgaggaag cagcaaaaat cgccgtcgac
    15121 aaagtaggaa gcggtacggc aacagattgg gatttggata aagatttagg tactacttat
    15181 tgggaagtca aagtaaaaga cggcaatcaa agtacatcag tcaagatcaa tagccaaaca
    15241 ggcgaagtac tgtcaactga acaagatgat taagaatttc ttcggaaaac aagagttgat
    15301 ttttctttta gtcatgatac actagacaaa ataacttgtt tgaaggagcg atttacttga
    15361 gtttagaaac gacctttgcc aatcatatct ttgccaatcc tttgatgaat gcatctggtg
    15421 tccactgcat gactacccag gaattagatg aactggctca ttcagaagca ggagcattta
    15481 tcactaagag ctgtacgatc aatgaacgaa aaggaaatcc cgaaccacgc tattttgatg
    15541 ttcctttagg gagtatcaat tctatgggac ttccaaactt aggtttttct tattatctag
    15601 agtatgctct tgcgtatgaa aaagtgcagg aaaaccaaaa tcaaccgtta tttttttcca
    15661 ttgccgggat gagtgtccag gaaaacttgg aaatgcttga gaaaatagaa aaaagtgggt
    15721 ttaatgggat cactgaatta aatttatctt gtcccaatgt gcctggaaaa ccacaactag
    15781 cttacgattt tgaggccact tatgaaacac tgaaagaagt attcagcatc ttttctaagc
    15841 cgctaggaat caaattgcct ccttattttg attttgctca ttttgatcag atggctgata
    15901 tcttgaatca gtttccattg acctatgtca atgctatcaa cagtgtgggc aatggtttgt
    15961 atatcgatac agaacaagaa gcagtagtca tcaaaccaaa agaagggttc ggcggcattg
    16021 gcggagagta tatcaagccg actgctttgg cgaatgttcg cgctttttat acacgtttga
    16081 aaccagaaat ccaaatcatt ggtacaggtg ggattcggac aggacaagat gcatttgaac
    16141 atctgctatg tggcgcaagc atgttacaga ttggtaccga gcttcataaa gaagggccgg
    16201 aaattttttc tagaatcata aaagaattaa cgcagattat gtcagaaaaa ggatatacgt
    16261 caattgacga atttaaaggc aaattacgca ccatttcata gcaaagagac gaaggtcatg
    16321 attgatcttc gtcttttttt ataaaaaaca ctcgtctgtt cattcctgtg caagcgtact
    16381 cattgtatgt tagacgaact tgtgggaaaa taacagaggc aaaaaggagg ataatatgaa
    16441 tgatgcaaca gaattagcaa aagagcttaa agaaaaagaa tttagttttc aagaactagt
    16501 tacggatata cagaaaaaaa taaagaagca aaatcatgaa ttgaatgcgt ttgtcgcgct
    16561 tgaaccattc gagagtaatt cccacgagct attgattccc cagtcagaaa aagggccatt
    16621 ggctggtatc ccatttcctt taaaaatgct cggtcaagaa aaaaagggat ggctagctac
    16681 ttcaggatct cgtttatttg aaacacaccg agcttctcat acatctaatt atgtgcggca
    16741 agcagaagcg ataggtctag ttccttttgg acaaaccaat gcgccagagt ttggtttcaa
    16801 aaatattaca gacccagtca tctatggccc agcaagaaac ccttggaacc ttgaatgtac
    16861 accgggcgga tcaagtggag gagctgctgc tgcagtttca tcaggaatcg ttcctttagc
    16921 tggtgctagt gatggcggtg gttctattag aatacccgct tcgttttgcg gattgattgg
    16981 gctgaaacct tcgcgaggga cgatgccagt tggtccatat gcttggagag gatggcaagg
    17041 agctgctatt gatttcggct tgacagtttc tatgagagac acggaggctt tattttatgg
    17101 gatgagaagt atccattccg gtgcgccata tcaagtatcg ccagttgagt ggcaaacaca
    17161 tccaagaaaa aaacggttga agatagcttt ttgtgttgat tcaccgatcc actcacagat
    17221 tagtgaagag gcagtacttg ctgtaaaaaa agctgtagat ttccttggaa aagaagggca
    17281 tgatttgaca gagattcctt acccactaga tggtcgtaaa ctgatcacgt cttattatca
    17341 gatgaatggc gcagaaacag cagcaatgat ccgctcgatt gaaacaggca tccagcgagc
    17401 tgtcagaaaa gaagaacttg aacccatttc ttggactttg ttccaatatg gtcagaagct
    17461 ctctgccgct acttacatcg attctctgca tgtatgggat gaagcagcaa ttgtcatgga
    17521 aaacctacat caagaatacg atctattcct ttcaccaacg actgcattta ccgcccctaa
    17581 aatcagcgaa gatttgcaaa gtgatgcgat aagagcacag atggaacaca tcagtgattg
    17641 ttctgaagta gaaggtttag acgttgtttc ttccatgttt gaggagagct tgaagatcac
    17701 accttataca cagttggcta atctgactgg acaaccagca atcagcttgc ctactcattt
    17761 gacaaaagaa aacatgcctc taggaatcca atttatggct gccagaggaa gagaagattt
    17821 gctatttcaa atcggctatc tatttgaagc aaatcatcag ttccatttac caaatccatg
    17881 gaaatagcag aaacaaggag aagaagaatg aagaaaaaaa gattgtttgc tctaataact
    17941 agtgcctttt ttattttaag cgcttgcggt acagctgccg ctaatcagga agataacagc
    18001 tggagtaatg tagaaaaaaa gaaagaatta gtagtagcga cttctggcac actgtttccc
    18061 gcatcttatt ataatgatca aaatgaatta gttggttatg atgtagattt ggcaaaagag
    18121 gtggctaaac ggttaaatgt caaaatctcc tttaaagaat acaacgtgga tggacaaatc
    18181 acttctgttt caaaaagaga atcagatttt gctgcgaacg attttggtat cacaaaagaa
    18241 agagaaaaaa aattctccct ttcagagact ttcaaatatt catttgacag tatgatcgta
    18301 cgaaaaaaag atgattcagg aatccactca cttgaagatc ttaaagggaa aaaagcagct
    18361 ggcgaaccca atactagcta tatgcgttta gcggaaaaac acggagctaa attagtcaca
    18421 tatgacaatg ccacgaatga tcagtattta agcgatgtag caaatggtcg aacagatgtt
    18481 attctaaatg attattatct gcagaaaatg gccgtatctg ctttaccaga tatccctgta
    18541 aaaatactgg agaacgttta tttcaatgaa aacgaaagtg gttttttatt gaaagaagga
    18601 aatgatgctc tgaaaaagaa aatcgatggc gtattaaaag agatgaaaga agatgggacg
    18661 acaaagaaaa tatctgaaaa atattttcaa actgatgttt cagtcaaacc gaaagaaacg
    18721 atggctgagt cggtgagtga gtgatgtact ttcctctaat tgatttttcc ctcatgtgga
    18781 cttcacttcc tttcgtactg caaggcttgg tatacacatt agggattggc ttcgtgtcgt
    18841 ttcttttagg aaatctggta gggttgcttt tgacagtttt aggtcttttg gattggctgc
    18901 ctctgaatgt atttattcgt ttttatttat cgttctttag aggcattccc gcactagtcc
    18961 tgctgtttct gttatatttt gggttacctt atcaattaag tgccttaacg gcttcagtca
    19021 tctgctttac gattaccagc agtgcattta ttggtgaaat ctatcgagga tcactagcag
    19081 gcgtttcttc aggtcagtgg gacgctggtt tagcttctgg tttttctttc tttggggtat
    19141 tgaaatatat cgttttgccg caagcttttc gaatctcagt accggcactc agtaacgtag
    19201 ctatggatct agtgaaaggt acttcgttag cagcaacaat cacagtgcca gaaatatttc
    19261 aaaaagcaaa gatcataggc ggtcggacat tcgattattt ttctctctat attttagttg
    19321 cactgattta ttgggtcatt tgtatcggga tcgaaaaagt gcaacagtat ttagaaaaac
    19381 gttctatgca tcagtcaatc tgaaaaat
//
LOCUS       DBNICI010000041        18642 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.041, whole genome shotgun sequence.
ACCESSION   DBNICI010000041 DBNICI010000000
VERSION     DBNICI010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.041"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(185..1168)
                     /locus_tag="AC1ZCC_001179"
     CDS             complement(185..1168)
                     /locus_tag="AC1ZCC_001179"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIQ1629443.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            1546..2937
                     /locus_tag="AC1ZCC_001180"
     CDS             1546..2937
                     /locus_tag="AC1ZCC_001180"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIQ1629444.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            2953..3993
                     /locus_tag="AC1ZCC_001181"
     CDS             2953..3993
                     /locus_tag="AC1ZCC_001181"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIQ1629445.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            4012..5097
                     /locus_tag="AC1ZCC_001182"
     CDS             4012..5097
                     /locus_tag="AC1ZCC_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HIQ1629446.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            5130..6092
                     /locus_tag="AC1ZCC_001183"
     CDS             5130..6092
                     /locus_tag="AC1ZCC_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HIQ1629447.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            6085..7512
                     /locus_tag="AC1ZCC_001184"
     CDS             6085..7512
                     /locus_tag="AC1ZCC_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIQ1629448.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            7514..8584
                     /locus_tag="AC1ZCC_001185"
     CDS             7514..8584
                     /locus_tag="AC1ZCC_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HIQ1629449.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            8571..9992
                     /locus_tag="AC1ZCC_001186"
     CDS             8571..9992
                     /locus_tag="AC1ZCC_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HIQ1629450.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            10005..11012
                     /locus_tag="AC1ZCC_001187"
     CDS             10005..11012
                     /locus_tag="AC1ZCC_001187"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HIQ1629451.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            11024..12028
                     /gene="neuB"
                     /locus_tag="AC1ZCC_001188"
     CDS             11024..12028
                     /gene="neuB"
                     /locus_tag="AC1ZCC_001188"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HIQ1629452.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            12025..13173
                     /gene="neuC"
                     /locus_tag="AC1ZCC_001189"
     CDS             12025..13173
                     /gene="neuC"
                     /locus_tag="AC1ZCC_001189"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HIQ1629453.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            13146..13823
                     /locus_tag="AC1ZCC_001190"
     CDS             13146..13823
                     /locus_tag="AC1ZCC_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HIQ1629454.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            13774..14955
                     /locus_tag="AC1ZCC_001191"
     CDS             13774..14955
                     /locus_tag="AC1ZCC_001191"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIQ1629455.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            14968..15945
                     /locus_tag="AC1ZCC_001192"
     CDS             14968..15945
                     /locus_tag="AC1ZCC_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIQ1629456.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            15945..16853
                     /locus_tag="AC1ZCC_001193"
     CDS             15945..16853
                     /locus_tag="AC1ZCC_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HIQ1629457.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            16854..17606
                     /locus_tag="AC1ZCC_001194"
     CDS             16854..17606
                     /locus_tag="AC1ZCC_001194"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HIQ1629458.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            17611..18558
                     /locus_tag="AC1ZCC_001195"
     CDS             17611..18558
                     /locus_tag="AC1ZCC_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIQ1629459.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT         6633 a         2470 c         3324 g         6215 t
ORIGIN      
        1 gcttatttaa gaagaagttg cttctgctcc caccgtttat tcgtttttaa agaatgagac
       61 aaaagtgttt cttacttttg tcccacgctc ttattcatgc tcagagcgcc aatagataca
      121 aaatcatagc caaagtttca cgaaccctag ctgtcaaaga atcgatattt tattttaata
      181 taggctattt taaaataaat ggtttaaggt attcctctac aaaagccact ctatcaatta
      241 tcatgggttt aaaataagaa gtgatggaga taaccagatt gttcttagcg tcaatataaa
      301 gaacatttcc actattacca attgctgaat atatttcgtt ctcagaatct aatacccacc
      361 ataaatagcc atatctcata ttttgtaagt cttctaaagc actggttcta ggtttgaaca
      421 tctcgtttat ccacatgggt ggtacgattt gttttccttt atacatccct ttatttaggc
      481 ataatacacc aatttttgcc agatcacttg ccgataaaca caaaccatat cctgccgtcc
      541 cgatatttag tggatcacaa aaccagacat tggttttagg agacttatcc attgtaaact
      601 gcttatgttc ttcagctgtt tcagcataat aattgacatg gtttggtatc cctataggat
      661 caaatagata cttattggca aaatcaacag ttgttagctt acttgtctga tacaacaagc
      721 cagttaatat gtgtaaacag acagtttgat agttgaattc gccggttata ccttttcttc
      781 ctcccaataa atctagtgat gtttttgtcc aatcttcact tgaacagact tttgtccatg
      841 gatcgccttt acatttgtag ggtgctttca ttgttagcaa gtgcttgatt gtaatagcgt
      901 aaatagtttt ctccccgcgt ttcactttat actctggaaa ataatctaat actttatcat
      961 cgatactttt gattaatcct tgctctaaag caatgccaat cagcaaagat acaatacttt
     1021 tcgttgctga catgatatga caagtatcgt cttttttata accattccat tcatctgtat
     1081 aaacttctac cccatcctta tatgcgacaa tttgacatat atttggttgt cgaaaaccaa
     1141 taaaatcatg caactcttgt ttattcataa gatattacct cactttcttc tataagcata
     1201 atgtcattaa aaatcgattt caattttttc gattcttcac gtaatacagg tattcttatg
     1261 gactagacag cgctttggga agatggattc tctgaaaaat ataaaggttc actttatgag
     1321 tggataaaaa aagcagcaaa tacttcgact tctttaattt accaacatta gtgaaaatcg
     1381 attcatctgg agatttgtaa tttagagtcc ttgtcttgat tagaggtgta taaagctttt
     1441 tattagacat ttttaaattc cattacaatt tgcttataaa ctaggttttt aaaggtgact
     1501 atggtaagat aatagagata ttattttgtg attaggagtg tatatatgaa taaaaatgga
     1561 gaatggagtg ctgcacgacg gatattcatt atattaatgg atgttattgt atacaatgca
     1621 gcaatatata gtagtttctt actgaagttt cgtggagaga tcccattacg aaatttagaa
     1681 actttccaac actcagcaat tattatttca gtgattttta ttgcgttgaa cattttgttg
     1741 ggtgcatatg ttttttataa tcgaatgatc agtgacatcg tgtttgtcac agtcatcggt
     1801 caggtattga tgacactagg gatcatggta gtgacttttg cgggccgctg gtttgctttt
     1861 ccacgtcttg ttatcttact ttcatttgtt ctcggtacgc ttttcttaag tatctatcgt
     1921 attctcgtat ataaacttta tttacgcgta agtcgagata aaaaggtcgt cattattggg
     1981 ttagaaaagg atgtcgcacc agcaatccaa aattttggtt caaagaaaaa caacaaacat
     2041 aaagtcgaag cagtcattct tgataactat tatgcaaaca tcaaaaatat gattaaagac
     2101 atcgatattg tctatcttgc ttctcatatc gaagaaactg aaaaaatcaa aatctatcaa
     2161 ttagttacta aaaatgaaaa aaaattgttt ttgaatacaa catttgaaaa cttgacattg
     2221 atcaatccaa atattatgaa tttcgaagat gaaagtatta ttgaagcatc tggatttaga
     2281 atccaacctg aatatgaact attcaaacgt ttgtttgata tcatcgtttc acttgtttta
     2341 ttagttattg cttcaccatt tatgttattc acagcaattt tagtcaaagt gacatcaccg
     2401 ggaccaatca tctataaaca agtgcggatc acaaaaaatc aaaaggaatt ttcaatctat
     2461 aaattccgct cgatgaccgc cacagccgaa gcaaaatctg gaccagtttt agctaagagc
     2521 aatgatgcac gagtaacgcc tgtaggtaaa tttattcgag ctgtccgctt tgatgaatta
     2581 ccacaaatct tcaatgtgct gaaaggcgat atgtcgatcg taggaccacg tcctgaacgt
     2641 cctttcttcg tcgatcagtt caatgcagaa aacccttatt actacttacg gcataatgtt
     2701 cgtgctggta tcactgggta tgcacaagtc tatggaaaat atgtctctga ttacaacagt
     2761 aaattacgtt ttgatttact ttatatcaaa aaatattcat tgatgcttga tttaaagatc
     2821 ttgctgcaaa ccatcaagat cctatttgat aaaatgtcct ctcaaggact tgaagaagac
     2881 gaaaatgtga tctcattggc agaaattgct tttccgaatg aaaaaatctt tcgctagttg
     2941 ggaggcgtaa aaatgaacaa tccgaaattc agcataatcg taccagtata caatgcagaa
     3001 gcaactattg gtcgaacgat tgatattcta aaagaattgg attatgaaga gtacgaagtc
     3061 gttttagtaa atgatggatc aacagatcaa acacaggaaa tcattcaaga actgattgaa
     3121 aatgatgaac gttttgaatt gattactacg accaatcaag ggcccggatt agcaagaaac
     3181 gaaggaataa gacagtcaaa aggagaatat ctcttattct tcgatgcaga tgacacgcca
     3241 gtaaaaacga tcttgtcaga ttatgatcat ttgctagaaa agagtccaaa agcggatctt
     3301 gttgttagtt cgtttgtttt tcgtacaatg gacgagggaa gaatcgtttc cgaaaaagaa
     3361 aacttagtta cagaatgtca ctatctaact cacgaagagt ttataaaaga catgtatcag
     3421 ttaatgaatg aacagttgat gtatgttgta tggaacaaat gctatcgtag cgatattctg
     3481 aaaaataacc aaattctatt taaaacttat aatagttgtg aggatcgaat atttaactta
     3541 cattattatg aatactgtca tcaagtgatc atgaacccaa aagtagaata tatttatgaa
     3601 tttgaaggtg ggaaggggat cactaatcaa taccgaccta acaagttcga aacatttaaa
     3661 gaattctatt cattagcaaa tactgtaaca gacgatatta ataaagacgg tatggcggcg
     3721 cttcttttaa aagggacaac ttcagttatt ttttctattt atagtacctc tgcaagaact
     3781 tcaaaagaga agaaagacga agcaaagcag atattgagtg atccagtgat ccgagaagcc
     3841 aagcaaattg ctaagacgga ttcttcagca aaaaaaataa caaagttgct ttacaatatg
     3901 ccagctccac tatttttatc tgctgtaaaa gttggaagct tcgttgaagt gaaaatgcca
     3961 gggttaatgg ctgctttgaa gcgagtatat tagttaatgg aggaaaatat aatgaaaaga
     4021 attttgcatt ttcagggacg aatgggttta ggtggagcag agtctttcat gatgaattta
     4081 tatcgtaaaa ttgatcgtac aaactatcaa tttgattttt tgatttatga agattatgca
     4141 gatgttcaag actatcattc agagattgaa agattaggag gccgtatttt tgttgtacct
     4201 aatcctaaga aaaatatttt taaatattta atagaagtga ataagttact aaaaaaagaa
     4261 tctttttcta tagttcacaa tcaggtttat tttggcgggg gaattaattt atggttagcc
     4321 aaaaagaatg gaatcagaca gagaattgct catagccatg caactgaaga tggaaaatct
     4381 caaaatattg taatgaatgt attgcgtaaa tttttaacca aattgttgtt acaaaatgca
     4441 acagactacc ttgccgttag tcaagaagct ggtgaaagtc tgtttcaaaa ccaccccttt
     4501 gagatagttc ataatggtat tgatttagag ctttatagta aaaattcaga agctaaggtt
     4561 aataagagaa aagaattgga tatctcaatg agtacttttg ttgtgggaaa cattggacga
     4621 ttagagaaac aaaaaaatca atcttatttg ttagagattt ttttagaaat actaaaaatg
     4681 aattctgatt cttacttgat tttagtaggt gaagggtcac ttagaaataa actgaaagcc
     4741 aaagcagtag aattggatat agagaaaaga gtaaaatttt taggagaaag aaacgatatt
     4801 cccgagttat tatctgtgat ggatgttttt gtaatgcctt ctctttatga aggtctgcca
     4861 atggttggat tagaagcaca agcagcaaaa ttaaaattag tcttagcaga tactatttcc
     4921 aaagatacaa agttaacaaa taatgtggat tttgtatcat tagatgaaac accacaagaa
     4981 tgggcaaaaa aagtaataaa agagccgtat gctaataaaa taacacaaga actaaagagt
     5041 tatgatgttt cgtacactac tgaacaaatg gagagtatat atagtagaga tcattaaaaa
     5101 tgttcaagat attttaggag tgattatttt tgaaaatatg ttatttgata ttggcataca
     5161 atgatattgg caacttaaag agattggtca atcgattgaa cgaaaatgca gtttttatta
     5221 ttcatataga taaaaaaact gatatagaac catttattga agcatttaaa tcatatgata
     5281 atgttgaatt tttatgtaat aaaaaccgtg ttaaaattta ttggggtggt ttttccattg
     5341 tacaagcaga aatcaattta gtaaagagag cattacaaaa tgagaaatat ttaaaatatg
     5401 tattattatc tggtgctgat tatccaatca aagataatga gtatatttat aattatttta
     5461 aaaagaatag ttcagtggag tttattcgtg gaatagatct agatcaaatt aaacataaag
     5521 aattttatta taaacatata gatgtatatc aaaaacatga ttatcctaga attaatagga
     5581 ataacacaac tgcatttaaa atttttagag ctataattaa tcgttgtctg aggatgatta
     5641 aacttccccc aaaaattaga caccataaat ttgatttgta tcatggttct caatggtggg
     5701 ctttgtcgaa agaatgccta acagaattaa tacaaatgta tgaacaaaat caacaagact
     5761 acctgaattt taaaataggt atgtttgccc cagatgaaaa attttttcat acactttttt
     5821 ttaattcctc gttcaaaaat aaaaatgtta ttggaggacc agatttacca cttgaattga
     5881 aaaatattga agaaactact ttacaaactt cgaaattagc aaatattcat attattgatc
     5941 cttccatgaa caaatggttt gatgaaaggg atatagatca aataaaagaa agtgaaaaat
     6001 tatttgtaag aaaagttaaa tcggattact ctgataaact attaaataga attgataaag
     6061 aaattttgaa agttggaaat atagatgaat agaatagtac ctgttttaga gaagagttta
     6121 ttaagtcgag atgcgtttcg agtaataaat ttaatcattt gtctgcttta ttttatgcct
     6181 agtactttta atgtaactaa tatcccgatg aaaatagctt ttgcttgggg tggaattata
     6241 gttttgtatg aggtttttat aaagaaaaat ttttttaaaa tgcgtttttc ttggctactg
     6301 attggagcaa tgattagttt tatcttaact ataatcatta acagagaaaa ttttttaatt
     6361 ccatctattt ataattttgg atatttgctg attactttga tagtaatttt tcctgcagac
     6421 ttttcacaaa gtgaagagga aaaaaagaaa aaaatggtgt attttaataa tgttttcatt
     6481 atcataattt ttttagctgc gctaatatct atctatcaat ttattttttt gatttcttat
     6541 catgtcccaa caggaacgcc aggattactt gcaagacaag gttttattga acatcggtta
     6601 tttggtgtct atacaagtcc aaatgttgga gctatgtttg gatatacttc tattatttta
     6661 agtttaatta gtgtattaat ttcaaaaata acaaaaacga ttcgtgtttt ttatatgatg
     6721 aattttatcg tacaaatatt ttatattaca ctttcttcgt ctagaggtgc ccaggtagta
     6781 atttgttctt ttttagtatt atttcttttg ttgtttgcca attcaaaatt tagaaagagc
     6841 attagagaag tactacctgt aaaaatcaaa tggatggtaa gcatattatt agttctattg
     6901 gtatattttt cttttggtac taattatgct aaggattttt tagcaacaat tcctgtatcg
     6961 attgatagaa aagtagaagt tatagagtca aatgaatcag ataaaactcc acttccagaa
     7021 aaagaagtaa tattacagca ttctagtgat aactccgaaa tttcagctgg tcgttttacg
     7081 atttggaaag ctgcgcttaa agttataaga ccaactttgt tatttggtta tggagagacc
     7141 gatttctatg ggaatagcga tgcaaaattt ttgaaaaaag tagatcttga tactacagat
     7201 gtgaatgaac taaaaagagc acatggaaac atgcataatg gatttcttca agtacttgta
     7261 tcaagtggta taattgcttt tatatgtatc tatgctttct atgtgttaaa tatctttagt
     7321 ttactcaaaa cattttttag aagtaaagtg aattatgcgt ttcttggtat tgttttaatt
     7381 tttatactat ctatttttat agatgaaatg gtggaggctc atgtactttt caataagcgc
     7441 gatgtcattt caattgtttt ctggtattat ttgggaagca tttcttactt gtatttaaaa
     7501 aaggagaatt aatatgaaga cagctttcat tgcttggact cctttacatt tgattaatat
     7561 attaaatgtt tatcaaaatt tttaccgaga atcaaaagga gatttattta tttattctga
     7621 atttgaaaac gctagaagat atagtgagcg tataaaagaa ttagggctat ttaatgatgt
     7681 tgtttttgtg aatcaccgga aaatcggttc ttcctttaaa cgtaaaatta acttgttaac
     7741 taaccataat caatttttct cggatgattt atccgtttat gatgaaattt ttatacaggg
     7801 ggaaaattat ttcacaaaaa tacttttctc caatgtttac aaaaaaaatc cagatgtaaa
     7861 actaaattat atagaagatg gtttgggtac ttatttgaag aagagtatat tttcaacaag
     7921 taaaaacgtt caaagagttt ttaaaatatt aaataaaaat agcatcttca aaaaaaaaat
     7981 tgacgattat tttgtctatt gtccggaatt gttactttat gatgtaaaaa atataaaaaa
     8041 aattcccaaa atagatgaaa acagtaaatt gatgaaaaac atacaatcaa tctttgggat
     8101 agaagaagtt tttgtcggag ataaatgtat cttttttgat caacctttat tatctgatgg
     8161 ttatggcata gatgaaacag atattttcgc taaagtaaat agtatatttt ctggaaaaaa
     8221 agaaacttta attaagcttc atcctagagc aaggacaaat cgttatggtg aaaaaaacat
     8281 attgaattcc agtttaccgt ttgaagtctt gctacttgaa aatgacttct cttcaacact
     8341 tttgattagt cctatatcta caatttcttt ttcaccatct atgatgtttg aaaagacgat
     8401 caactctatt atgttggcta aattaatttt aaatgaatat acagcattat ctgaagataa
     8461 aaaagaaaca ttgacaaata ttgttctttt ctgtgaacat tataaccgtc tatcagaaga
     8521 aaaaatttta ctcccagaaa actgggcaga attaagaaag ttggtagagt atgggattat
     8581 ttaacacact ttccaagtca aaattagtta agtccagttt ctggtatact atagggaatt
     8641 tttttattaa gggaatttca ttcataacta ttcctatttt tgtaaatatt actactcaag
     8701 cagaatatgg tttgattaat aatttcactt catttgtatc aattttttcg attcttgttg
     8761 ggctaagcct aaattcggca attaataatg caagttttga ctataaagat gacataaatg
     8821 aatttatgtc atcggtactt tctctagcta cactttcgtt aattgtgttc ttattaatag
     8881 ggaacatata ttttttgttt tcaaatgctt tttttgatat gaaccaactt gtatttaatt
     8941 tgatgttatt acaaagtttt gctagttttg taattagtta tatccagtct tattttacga
     9001 ttaactttaa ccattttaaa tatttgattt tatcttttat atctactttt ttgaatattg
     9061 caatctctgt agctttgatg cagacattat attttgatga taaatatgtt ggcagagtag
     9121 tcggcggaac actgtcattt gtgttgatag gtttagtaat ttatttaaga ataatgataa
     9181 agggtgctca tttttttgat aaaaaacagt ggagatacgc tttaaaaatc tctttgccgc
     9241 taatcccaca ttccttagca aatatagttt tagcacaatt cgatagaatt atgattaatt
     9301 cttatttagg tcctgctgcg gggggaatat attcctacat tagtaacata ggtattattc
     9361 taagcgtaat atggatgtct actaataacg cctgggttcc atggttctat ggtgagatga
     9421 gtaaaaataa tgaaaaaaaa gtgaagaaag ttagtaacta ttatttagta gtctttactt
     9481 tgattacgat gattgtaatg gtagtttcga ttgatttagc aaaatttatg gctccgaaag
     9541 actattattc tgggttatct ttagtggttc cgataacctt aggctatttc tttcagtttt
     9601 tatatagttt accagttaat gctgaatttt atgagaaaaa aacatcgttt attgcgattg
     9661 gtacagtagc aagtgctata gttaatatac tattaaacat gattttcata cctaggttag
     9721 gaataattgc tgctggatac acaaccgtat ttacgtattt tttacttttt atttttcatt
     9781 ataatatcgc taaaaaaatt acgaataaac aattatttga cacaaaaaaa atatgtatca
     9841 taataatttt tgtatcaatt ttttctttga tacttcatat tttaatcaat caaatgttga
     9901 ttcgttacgt tttgacatca ttattgataa ttagtttagt tatatatttt ttaaagatta
     9961 agaatcgaat tatagatgac tataattcat aggaggagat ataagtgttt aaaggaaaaa
    10021 caatattagt aactggcgga actggttcca ttggaagtga aattgttcgt cagcttttaa
    10081 aatatgaacc aaaagctatc agaatttata gtagaggtga agaaaaacaa ttttatatgc
    10141 aacaagagct ctcaaatcac actaatttga gatttttaat tggtgatatc agagactctc
    10201 aaaggattga ttatgcatgt cgtgatgtgg atattatttt tcatgctgcc gcaatgaaac
    10261 acgttccagc aagtgaatat aatcctatgg aagctgtaaa aacaaatgtg atgggaactc
    10321 aaaatgtaat agatgcagcg atcaaaaata atgtaaaaca ttttgtaggt atcagtacag
    10381 ataaagtagt gagtcctact aacaccatgg gtgcgacaaa gttattgagt gaaaaattaa
    10441 taatagctgc caatcagtat aaaggaaaaa cagaaacaat attttcttgt gtaaggtttg
    10501 gaaatgtcat ggggtcaagt ggatctgtta ttcctctttt tagtaaacaa ataaaaaaca
    10561 ataaagatat tactttgaca gatgttgata tgacacgatt tatgatgtca ataccggatg
    10621 ctgcaagatt agttctgtct gctgctatga attcaaaatt tggagaaaca tatgtcttaa
    10681 aaatgccggc aatcaatatt ttaactttag ctcaatcatt aataaaaata tataatgata
    10741 cacatgatga aaaatataga ggtgaaataa aagttgtagg tatcaggcca ggagaaaaat
    10801 tatatgaaga gctaatgact acagacgaaa ttgaacgctc atatgaaaat gataaaatgt
    10861 atatcattcc accacactat catgctactg acgaactgta caaagattat gaaaaaatta
    10921 aaatcactgc atactcttca gatgcaacag aattaatgag tggacaggaa tttattagaa
    10981 tcgcaaaaga gaataatgtc gtgacttggt aaggagtttt atgatgaaat cagaagtata
    11041 tgtaattgca gaaattggtg taaaccataa tggaagtcta gatttagctt tagaaagtat
    11101 tgataaagct aaagaatgtg gagctaacgc ggttaaattt caaacattta aaacgaataa
    11161 gctagtttcc aaaaaggctc caatggcaaa atatcagaag gataatctta aagaggacag
    11221 ttctcaattt gaaatgctaa aaaaattaga attatctcaa tctgatttta ttagaataaa
    11281 agattattgt atacaaaata aaattgattt tttgtctact ccttttgatg aagaaagtgc
    11341 agactttcta aaacaaattg gtgtacaagg atttaaaatt ggctcaggag atttaacaaa
    11401 tttacctttt cttagaaaaa tagatatgta tgggttgcca gttttattat ctacaggtat
    11461 ggctgatatt catgaggtca gtgaggctgt agattgtttc atagatagtc cagttactat
    11521 tttacattgt acttcaaatt atccggctag ttctgaagat attaatttat tggcgattaa
    11581 cacattaaaa gaaatgttcg ggatacccgt tgggtactcc gatcattcat tgggatatga
    11641 tgttggtatt tgtgctgttg cgatgggtgc taaggtaatt gagaaacatt ttaccttgga
    11701 taaggaacta cctggtccag atcataaagc ttctttagat cctcaggaat ttgcagattt
    11761 tgtaaagcat attagaaaca ctgaaatatt tttaggtgat ggtgttaagc gagcaatgcc
    11821 ttctgaactt agtacaaaag aagtatcaag aaaaagccta gtggtcactg agaatttaaa
    11881 tgtgggagat attcttacag agaaatctct tacaataaaa cgtcctggaa atggaatagc
    11941 tcctaagttt ttatatcaat atattggcaa aaaagttaaa agaccaataa gtgcagatag
    12001 tgttctgctt gaggaagatg ttgaatgaaa aaaatactta tagtaagtgg aacacgagct
    12061 gattatggaa tatataaacc tgttgctgag aatttactac aagataattg ggatgtatca
    12121 tttgttgttt ctggaatgca tttatcccat aaatatggat ttactaaaga attgatctta
    12181 gctgacggat ttagaattag tggagaagtt caaagtttgt ttttagagaa caataaggga
    12241 aacatggctc ggtctgtggc attagagata aatggtttca caaatatatt tgaacaaact
    12301 agccctgatt ttgtattatt actaggggat agaggagaaa tgttggcagc tgcgatagct
    12361 tgtttgtatc ttggaatcaa aacagcacat attcacggag gcgaagtgtc tggtactgta
    12421 gatgaatcaa taagacatgc cataacgaaa atagcatctg ttcattttgc tgctacggaa
    12481 aaaagcaaag agcgtttaat aaaaatgggg gaagattctt ggagagtttt tcccgtcgga
    12541 gctcctagaa tagatactat tttaaataat agattaccta gttttagaga tataaaagaa
    12601 aaatatcatc taaaatttga aaagaaaaaa tacgttttgc aagtatttca tccagttgta
    12661 accgaactag aaaatataga aaaacaaatt ttagctttga tcaattcaat ggaacaacaa
    12721 tctcaaccaa ttatttgcat tttacctaat agtgatgctg gtagcgaaat tatcagaaaa
    12781 atgtaccaga aatatttaaa aacggatatt atttatatta cgaatttaga gccggaggaa
    12841 tacctgacta tattaaaaga gtgcaaattt atgatcggaa attcttcttc tgggataatt
    12901 gaagctgcat cattcaaaat accagtaata aatttaggaa ccagacaaaa tggacgagaa
    12961 caatctaaaa atattataaa tgcaaatcta aatgaaaatg aaattgaaac tgctttgaat
    13021 aaaatacaag atcctcagtt tataaaaagc ttgaaaaatg ttaagaacat ctacggtgac
    13081 ggcaacagtt ctagaagaat aacaaaaaca ttgaatcaaa tattgaatga tggggaagat
    13141 attaagtgga ttcaaaaaca aatagcttat taaaatatac tagtgatcca tacattatta
    13201 ttggggctgg cggacacgca aaagtcatta ttgaaacctt aagattagaa aataagttaa
    13261 tatatggatt ttttgatgat aagcaaaata aaatgcaaga tctgaaatat cttgggaaaa
    13321 taaacgaatt gaatagctgg gcggatagca aaaaaaaaat ctataaattt attgtagcta
    13381 ttggtgactg tgtagtaaga aaaaagatta taaaaaaatt gaatatgcca agtgagcaat
    13441 atggaatagc tgttcatcca agtgctatag tggcaaaaga aatttcaatt ggttatggaa
    13501 ctgttatttt tgcgaatgct gtaattaacc cagatgctgt aattggagag catgctataa
    13561 taaacacagg tagcattgtt gagcatgatt gtagaattaa taactatgtt catttatctc
    13621 caggtgtatg cttagcgggt ggagtacatg taggtgtagg aactcaaata ggaattggtt
    13681 ctcaatgtat ccagatgaag aaaattggat cttggtgtat gataggcgca gggagcacta
    13741 ttgttaagga tattccctca cattccttag cttatggcaa tccagctaaa attaagaaag
    13801 aaggaattaa ttttgaattc tagaatttta ttatcaagtc ctcatatggg aggaacagaa
    13861 gaaaaatatg ttgctaaggc ttttaaagat aattggatag cgccattagg tgaaaatgtg
    13921 gataaatttg aagaaagtat tacaaaatat actggttcta agggcggagc ttctgcgtta
    13981 agttcaggta cagctgccat acatcttgcc ttaagactgc ttaatgttgg aaaaaacgat
    14041 attgtctttg ctccttcatt tacttttata gctacagtaa atcctatttt atatcaaggg
    14101 gctgtaccag tgttcattga ctcagaacct aatacatgga atatgagccc tgttgcacta
    14161 gagagcgcat tagaagaagc tgaaagaaac aaccaattac ctaaggcaat catcgtagtg
    14221 aatttatatg gtcaaatggc tgattatgaa cgaattttag ctattgcaaa ccaatttaat
    14281 gttccagtga tagaagatgc agcggaatcg ttaggttcta cttataaggg aatttcgtca
    14341 ggcttatttg ggacaattgg gatctattca tttaacggta ataagatcat cacaacctca
    14401 ggtggaggag ctctagtgtc tagtgataaa aaatatgttg agcattcaag atttttagca
    14461 acacaagcaa gagatcaagc accgtattat caacatagcg aaataggtta taattaccgt
    14521 ctaagcaata tttctgccgg tatcggacgt ggacaaatgg aagtattgaa tgatagagta
    14581 gctaaacgaa gagaaatttt caatcgatat aaggaagctt tttctggaat tgatagaatt
    14641 gaaatgatgc cagaagtgga tggcatggtg ccaaatcgtt ggttgtctac tttaacgatc
    14701 aatgattata attctattga tcaagtagtt aacgtaataa ataaactaaa tgaaaaaaac
    14761 attgaaacaa gagctttgtg gaaaccacta catttgcaac cagtatttca aggggtaaaa
    14821 atgtattacc atgataataa tcttagtgtg tcagaaaagt tatttaatag cggcttatgc
    14881 ttaccttctg gaagtaatat gacagatagt gaacaagaat ttgttattga tgagtttaaa
    14941 aaaatattca agtaggtgtg atattttatg aacataggaa ttattggttt aggttccatg
    15001 gggaaaagaa gacttagact cattcaagaa cactttaatg atattttact atgtggtgtt
    15061 gatactaatg aggcgagacg aaaggaagtt gaaaaggaat ttggaatacc aacttataat
    15121 agtctaacag aggcaaaaaa tttgtttgaa ataaattcag tttttgtttg tacatcccct
    15181 ataacccatg aatttattat aaaagaggct ttaaatttag attgtaatat ctttacggaa
    15241 atcaatttat taaatgaata ttatgatgaa ataatcgaag tagctaaaaa gaaagataga
    15301 ttactttatc tatcttctac atttttgaag agaaaagaaa ttcaatatat taagtctaaa
    15361 gtgacgcata ataaaaaaat ttcttatcaa taccatgtag gacagtattt acccgactgg
    15421 catccttggg aagattatcg caattttttt gtttcaaaca aaaaaactaa tgggtgtaga
    15481 gaaatattcg ctattgaatt accttggatt atagatacat ttgggaaagt taaatcgttt
    15541 actgtaatga cccagaaatt atccaatctt gatattgatt attcggacag tttcactctt
    15601 attattgagc atgaaactgg tatagttgga actttaagtg tagatgttgt ttcaagagta
    15661 gctaaaagaa atttaactat aattggtgaa aagattttaa tagaatggga aggtaacccg
    15721 gattctctat tttcatggaa tgaagaaact tctagtatga cagatattca attgtacgat
    15781 gaggttatgc atgataataa ttattcacat actattattg aagatgcata tttagaggag
    15841 attaaagaat ttttcacgct tttggaaaag aaaatggaac cccaatatac ttttgaaaaa
    15901 gataagtata ttattaactt aattaacgac attgaggtaa attaatgatg aaagtaattt
    15961 tttttgggtt agggtcaatt ggaaagagac atctaaaaaa tctaataagc tatggaaaaa
    16021 agaattctat taattttgat ataacagctt atagtagcag tggaaaaaaa gagtttgaag
    16081 aaataacgta tattcaaaat agagacgatt tggatacaca ttatgatata gcttttatta
    16141 caaatccgac ttttaagcat ctggaaacac taaatgtaat tgttgaaaaa gttgattaca
    16201 tatttttaga aaaacctgct ttttctgaga gtattgatct tactcaatat aattatgatt
    16261 acaaaaatat ttatgttgct gctccactga ggtataaaaa tatcatggaa gttgttcgta
    16321 atagtattca aagtaaaaaa gtatattctg taagagctat ttgttctact tatcttcctg
    16381 attggagaaa aggtactgat tatagaaata attattctgc ctttaaatct atggggggag
    16441 gagtagaatt agactgtata catgaattgg attatattat aaacctattt ggtttcccta
    16501 aatcagtaaa aaaacagttt ggtaaatatt cgaatcttga aattaacagt aatgatttag
    16561 caacatatat attgagatac gatgataaaa ttatagaaat tcatttagat tattttggaa
    16621 gagttcctac aagaaaattt caattgttta ctttagatga atgtatcaat gttgacttat
    16681 taaataataa aataagtttt gaagaaaaca ttgttaaaga aatgcctgaa tctgctaatg
    16741 atatgtatga gaaggagttg aaatatttct ttgaaaacgt agtaacaggc aaagaaaact
    16801 ggaatgattt atatcatgca aacgaagtat tgaaaatagc tgaggagaaa taaatggaaa
    16861 ttttatttac tatttgtggt agagctgggt ctaaggggtt taaaaataaa aatttgaaat
    16921 atttggcagg aaagccccta gtccatttcg ctttagcaat gatagatatc tatcaacaaa
    16981 aacatcctga acataattgc acgacttgtt tgaatacgga cagtaatgag ttagtagaat
    17041 tgacgactaa gttaaatccg tctgtgaaaa ttgtagaacg taaacctgaa ttagctacag
    17101 atactgtagg gaaaattgaa gtgatacgtg attcattctt acaactagaa agagaagata
    17161 atatatttga tttaattata gatttagatt tgacttcacc tttacgtgaa gtttctaatc
    17221 ttgaagaatt aatagatgaa catctaaaaa atttagataa agacgtctac ttttcagttg
    17281 tttctgcaag acgtaatccc tattttaata tggtaaaaag aattgaaaat gaagttacta
    17341 tagttaataa atcaagatac acatctaggc aacagacacc tgaagttttt gaaatgaatg
    17401 cctcaatgta ttcttttaaa cctgaatttc taaaaaaaag taattatatt tttgatgggc
    17461 aatgtgggat aataaaaatg agagattatc taattttaga tattgatagt gaagaagatt
    17521 ttaaatggtt agaatttttg tttcctaaat ttttagaaga ttcttcggag cttcaagata
    17581 tctataatta tttatatcgg aggtaaaagg atgagctgtt atcttatcac cggtggggct
    17641 ggcttcattg gttctacctt agccaatcat ttaggagaag aacatgtggt aatcgtcgtc
    17701 gatgacttat caatgggaaa agtagaaaat cttgatatga cgaaaaatat cacttttatt
    17761 gagggagatg tcgctgagaa atccttgatg gaagagatca tgaaagctaa taaatttgat
    17821 tatatctttc atttagcagc agttgctagt gtggcagatt cagttgcccg accagtagaa
    17881 acacatcgtg tgaattttga aagtgtatta atgattttag aattgatccg caagtatcaa
    17941 cctgatttaa aacggatcgt gtttagttct tcagcggctg tttatgggga tgaaccgact
    18001 ttaccaaaaa aagaagagtc cgtgattcgc ccattgacgc cttatgcggt tgataaattc
    18061 gctgcagaac aatatgtgct agattattgt catttatatg atgtacccgg tagcgcagtt
    18121 cgttttttca atgtctatgg tccaaatcaa aatccgaatt ctccatacag tggtgtgatt
    18181 tctattctcg tagatcgcta taagaaacaa atcgtaggag aagcaacttc atttacatta
    18241 tttggtgatg gaagccagtc tagagacttt gtttatatag atgatgtaat ccaagcatta
    18301 cttttagttg cgaatgagga agctgcactg gggaaacaat tcaatgttgg aacgggtaaa
    18361 gcaaccacac ttttggaatt gattcatgca atcaatgaaa ttctcggtgt agaattgaca
    18421 cttgaataca aagaagagcg ttcgggtgat atccatgact ctctagcaga tattactaag
    18481 atccagtcaa ttggctatca accgaaatat ggtattttaa gtggaatgac taattatttg
    18541 aaaactgaaa tcaattaaaa catactaaga taagattaga gggtaaattc aggctctttg
    18601 tcaataagga ctgatgagtt ttgtaaaatc aaatctggga ta
//
LOCUS       DBNICI010000042        18347 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.042, whole genome shotgun sequence.
ACCESSION   DBNICI010000042 DBNICI010000000
VERSION     DBNICI010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.042"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            156..2627
                     /locus_tag="AC1ZCC_001196"
     CDS             156..2627
                     /locus_tag="AC1ZCC_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIQ1629460.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2730..4466)
                     /locus_tag="AC1ZCC_001197"
     CDS             complement(2730..4466)
                     /locus_tag="AC1ZCC_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIQ1629461.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4463..5167)
                     /locus_tag="AC1ZCC_001198"
     CDS             complement(4463..5167)
                     /locus_tag="AC1ZCC_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIQ1629462.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5298..5801
                     /locus_tag="AC1ZCC_001199"
     CDS             5298..5801
                     /locus_tag="AC1ZCC_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HIQ1629463.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5761..6099
                     /locus_tag="AC1ZCC_001200"
     CDS             5761..6099
                     /locus_tag="AC1ZCC_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HIQ1629464.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6120..7538
                     /gene="ffh"
                     /locus_tag="AC1ZCC_001201"
     CDS             6120..7538
                     /gene="ffh"
                     /locus_tag="AC1ZCC_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIQ1629465.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7650..8228
                     /locus_tag="AC1ZCC_001202"
     CDS             7650..8228
                     /locus_tag="AC1ZCC_001202"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIQ1629466.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8232..8753
                     /locus_tag="AC1ZCC_001203"
     CDS             8232..8753
                     /locus_tag="AC1ZCC_001203"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIQ1629467.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8832..9386)
                     /locus_tag="AC1ZCC_001204"
     CDS             complement(8832..9386)
                     /locus_tag="AC1ZCC_001204"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase [NAD(P)H] activity
                     [Evidence IEA];
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HIQ1629468.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9639..9914
                     /gene="rpsP"
                     /locus_tag="AC1ZCC_001205"
     CDS             9639..9914
                     /gene="rpsP"
                     /locus_tag="AC1ZCC_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIQ1629469.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9926..10177
                     /locus_tag="AC1ZCC_001206"
     CDS             9926..10177
                     /locus_tag="AC1ZCC_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HIQ1629470.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10302..11093
                     /locus_tag="AC1ZCC_001207"
     CDS             10302..11093
                     /locus_tag="AC1ZCC_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629471.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11263..11784
                     /gene="rimM"
                     /locus_tag="AC1ZCC_001208"
     CDS             11263..11784
                     /gene="rimM"
                     /locus_tag="AC1ZCC_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIQ1629472.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11774..12535
                     /gene="trmD"
                     /locus_tag="AC1ZCC_001209"
     CDS             11774..12535
                     /gene="trmD"
                     /locus_tag="AC1ZCC_001209"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIQ1629473.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12671..13018
                     /gene="rplS"
                     /locus_tag="AC1ZCC_001210"
     CDS             12671..13018
                     /gene="rplS"
                     /locus_tag="AC1ZCC_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIQ1629474.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13150..14286)
                     /locus_tag="AC1ZCC_001211"
     CDS             complement(13150..14286)
                     /locus_tag="AC1ZCC_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIQ1629475.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPNLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKMPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVDFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPKGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(14403..15095)
                     /locus_tag="AC1ZCC_001212"
     CDS             complement(14403..15095)
                     /locus_tag="AC1ZCC_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629476.1"
                     /translation="MKKVILGLVFGAFFLTGCSQSNVVDNTGKTNEKDSSVTLSESTT
                     QSSKNSQEVDGPLLKVGQWTKEKRDTSGKIELIGTASPQSDIPLGDIGIYISNIKLLK
                     YSNYADPSAAGYYDANNHKDSEGNFYGLQIAFKVKNNSEVDYGYNGLAHAILDNGQQI
                     DFSSDDLMHTMTTNSFFKKTESKEFYSLAYLDPSKVNDITKVTIKTGVLYNPSDYSTV
                     AESAESTFNINR"
     gene            complement(15233..15886)
                     /locus_tag="AC1ZCC_001213"
     CDS             complement(15233..15886)
                     /locus_tag="AC1ZCC_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629477.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKVSKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQDTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     GQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(15936..16364)
                     /locus_tag="AC1ZCC_001214"
     CDS             complement(15936..16364)
                     /locus_tag="AC1ZCC_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HIQ1629478.1"
                     /translation="MEMDTINLVEELKRKYRSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIDELKNLYGLPESAYRFLI"
     gene            complement(16369..16743)
                     /locus_tag="AC1ZCC_001215"
     CDS             complement(16369..16743)
                     /locus_tag="AC1ZCC_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1629479.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            17028..17213
                     /locus_tag="AC1ZCC_001216"
     CDS             17028..17213
                     /locus_tag="AC1ZCC_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1629480.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALVGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            17226..17474
                     /locus_tag="AC1ZCC_001217"
     CDS             17226..17474
                     /locus_tag="AC1ZCC_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629481.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKITPKEFIEKHLVDL"
     gene            17505..17684
                     /locus_tag="AC1ZCC_001218"
     CDS             17505..17684
                     /locus_tag="AC1ZCC_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629482.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(17727..18197)
                     /locus_tag="AC1ZCC_001219"
     CDS             complement(17727..18197)
                     /locus_tag="AC1ZCC_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629483.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
BASE COUNT         5911 a         3248 c         3607 g         5581 t
ORIGIN      
        1 caacacaaaa tattatagaa cccttttttt ttgctatatg acaggaatga aaggtgtttg
       61 caagcttaat gtgaggaaca ctttccatat ctgttctata ctggatagga gaagtttgga
      121 aatatccaga aaacgaaatc aatcgaggta gccacatgaa gaataaatgg ataaatcgaa
      181 gtaaaaaaaa gaaagtaaca caaagatcat cagataaagt catccgtctt tttccatcgt
      241 ataaagaagg attaacggca gatcaagtca aggaacgaat agaaaaaggt gcagcaaata
      301 attcagtcga tcccacattc aaaacaaatc agcaaatcgt attggaaaac atatttacgt
      361 atttcaattt gatctttctt atcttagcca ttttgctttg tttagtagaa tcctataaga
      421 acttgacttt tttaccagtt atcatagcca atacaggaat cgccattttt caggaaattc
      481 gatccaaaaa gatcttggac gaactaaatg ttttaaatac agcaaaagtc aatgtcatac
      541 gagacgggat agaacaaaca atagatatag aagaccttgt aatagatgac attgttattt
      601 tagagactgg atatcaaatt ccagcagatg cagaggtcgt ggaagggaaa ctgcaagtta
      661 atgaggcttt attgacaggc gaagcagatg aaataaccaa agaaataggt gatccattaa
      721 tgtccggcag tttcattgtt tccggaaaag cttacgcccg attagacaaa gttggagcag
      781 attcgtatat ttcccagctg acagtcaaag ccaagacaat gggagaagga gaacagtcgg
      841 agatgattgc ttctcttaat caattgatca aatggattgg gatcatcatt attcctatcg
      901 gtatcctgct tttttctcaa agttactttt ttaatggaaa cacgatcaaa gaaagtgtcg
      961 ttgcaatgga agcagcattg atcggtatga tacctgaagg actgtacttg ctgacgacga
     1021 ttgcattagc attaagtgcg actaaactag caaagcaaag agttctttta cacaatatga
     1081 aaagcattga gacattggca agggtaaatg tcctttgtgt agataaaaca ggaacgatca
     1141 cagaaaataa aatgtctgtt caaaaaatga tcgtctctaa aaaacaagaa ataatcagtc
     1201 agtcagatca attggaagca tgcattgctg actatgcaaa agcgatggca ggagacaatg
     1261 caacaatgga agctgttaaa gcatatttta caaaaaccac tgatcaatcc tatcaccatg
     1321 ttattccttt ttcttctgtc caaaaattca gcagtgtagc gttggcagac aaggtgtatg
     1381 ttttaggagc gcccgaaatg gttttgcgcg atcaattgcc ggaatatgcg gaagagtttg
     1441 tttctttcgc tgaaaaaggc tatcgcgtgt tggtctttgg tgtgtacgat gggattcttg
     1501 aagaaccagt attgacagaa gcagtcacac cattaggcta tattctgatt gctaatccta
     1561 tcagaaaaga agcaagagcc acatttgatt acttcaaaaa acagcaagta gccatcaaag
     1621 tgatttctgg agataatccg ctcaccgtat ccaatgtagc tgtccaagca gggatttcac
     1681 atgctgatca gtacgttgat gcatctaaat tatcagaaga agaatatgaa gcagctgtcg
     1741 aaaaatatac agtttttgga cgcgtcaagc cagaacaaaa gaaaatcttc gttcaattga
     1801 taaagcgtaa caatacagta gctatgacag gtgatggcgt gaatgatatt ctagccatga
     1861 aagaagcaga ctgcagtata gcgatggctt ccggaaatga agccgccatg caggcctcac
     1921 aagtggtctt acttgattct gacttttcac gcatgccaga agttgttgca gaaggacggc
     1981 gcgttgtgaa caatattgag cgatcagcca gcttgttttt agtgaagaat attttctcct
     2041 ttttgctctc actgttttca gtgatctttg ctctgaccta tcctttagaa ccatcacaaa
     2101 tcacattgat tagtttgttt actatcggac ttccatcttt cttgcttgct ctagaagaaa
     2161 acaaaaagcg aatcagaggg aaattcatca tgaatgtgat ggaaaaagct gtgccaggtg
     2221 gactaacaga tatgatcgta gtcggtgcgt tggtgatctg cggggtgact ttgaatctaa
     2281 ataaaacaga tgtttctaca gcatctacca tgcttttgat tgcagtaggg ttcctcgttc
     2341 tctataaaat ctgttcaccg ctgaataaat tcagaagtca gatcattctt ttctgtgcta
     2401 gcggtaactt tttttcggtc atctttttgc ataagctgtt ttcaatcacc ggtatctcag
     2461 ctgtatcact tttgctgttg agcattctat ttttctcggc agattcgatt tttaggcatt
     2521 tgaccacatt ggtagaatat ttgtttgaac gtaaaaatga aaaagagcaa aagccgttta
     2581 gtcgcgtagc taaaaaattt ctatcgatat ttaaacgaga aaattgacga aaagtatctc
     2641 aaaaaagagt aaaaaagaac aaccactcgt gtatctttcg aagcaggatg agtcatccaa
     2701 ctttaaaacg cacgagcagt tgctcttttt tacattttcg gaatggtaac ggtgaaagtc
     2761 gaacctgttc caagatgact gtctacagta atcgtaccat taaggagttg cacgtagtct
     2821 tttacaatcg ctaaccctaa tccggtcccg cctgaatgtc ggcttcgcgc cttgtctacg
     2881 cggtaaaaac gctcaaagat ccgttcctga tcctcaatgt cgatacctat accaaagtct
     2941 tggacagaaa acgatagatc atctgttgcc tgatagcgga taataatctc gctgtcgctt
     3001 tttgaatatt ggacagcatt ttcaattaga tttttgataa ttggataaag gatatctgtt
     3061 ttagtagtaa atggttcgtt ctttccaatc aatcgaatcg tcaaccgttt cttttcgatg
     3121 attggctgat aattttgaac gatttgctgg aaataaggtt ctgtataaat tgtctggatc
     3181 tcgtaagaat tctcgccatc ttttgacagc tggatgattt cccgaatcag tttatctaac
     3241 cggatcgcat ctttttgcat gatctcaaga aaagaagtca gagtttgcgg gtcatctttt
     3301 gcaccatcca gtaaagtctc ggtaaaacca atcagtgaag tcacaggagt ttttaattcg
     3361 tgagagacgt tgccaacgaa atccttttgt agcttttcta aacgtcgcac tttcgttaag
     3421 tcatacgcca cccctagaat ctgtccggta ccttctgaat tagtaaagta gcgcaaactc
     3481 aagtctaatg tctgccgacc ttctgtaagg gtgatctcct gatggatcag cggatgctca
     3541 ggagtcactt gatgaatcag ctgaatgagt tttggctctt gaatgacttc tgtgtaccgc
     3601 tgttcaggtt gatagcttac cacgtttaaa tgtagctgca tctttggatt aagcagtagt
     3661 aaacgagaat cagcatcatc aatcaggaat acgccaatca tcagttcgtt cagtaacgta
     3721 tataattgct cttctgtggt agtacacgcc cgatacatct tactcatttg ttcgctcaac
     3781 gcattaatcg tctgatacaa ttcttcccat tgatcgggtg tttgcatgat tacttccgcc
     3841 tgattaggat tttgcaccat tttttttagt acgggcagca cggttttcaa tggctggttt
     3901 ttttgataga tcagataata gatcattcct gcaagaacaa ggaagaaaga cagaaagaag
     3961 aaaaagaccc aacggcgaaa actctcagtt ctcggtaaaa aaccgcttgt cggttctgcg
     4021 atacgtaaaa tggcttctaa ctggccactt tttttgactg gtaatgcgac atataaaaga
     4081 tcctcattca aagtagtact tttgcgcaaa gcgctaccta ggttcccacc attcaatacg
     4141 gctttgattt cgggacggga gtttcgctgt tcattcaatg tttcattgtt tgtgtcaaat
     4201 aagatctttc cagtagcatc cattaaagta atccgttcgt tcgactgatg gacgaattct
     4261 tccaaagcag ttttgttttg cgctgcttgg aggttttcga catctaattg ccggatcagc
     4321 agttgtgctt tttttgtcag gtagctttct tgctgttcca cgatctgctg ttgaaaataa
     4381 tgggaaatca attgccagcc accaaaaaac aaactaacta aaagaataag aaagaagaga
     4441 cgttcataaa tttttttcca tttcatcgtt tgggttcctg aaacttatag ccaaatcctc
     4501 taatagtcac caagtacttt gggtgttttg gatctcgctc gattttttct cgcaaatgac
     4561 taacgtgtac atctacgatt cggctttgtc ctgcgaagtc aaaattccaa atgcggtcta
     4621 gcaatgtgtc tcggtcgatc acacggtctt ttcgtttcat gaaataaaca agtaattcaa
     4681 attctttcgg tgttagttca atcagatggt cgtctacggt cacttgataa ttggtcaaat
     4741 cagcagtgat ttgtccaatc gatagatatt caggctcagc ttcatctttt gattgcttac
     4801 gaggttcaat tcgtctgaat attgctttca tacgggctaa tacttcgcga ggactaaatg
     4861 gtttggtcaa atagtcgtca gcacctattt ctaggccaat gatccgatcc acttggtcat
     4921 ctttagccgt cagcatcaaa atcggtgttt catttttttc ttgacgcagc cgctgagtga
     4981 tctccatgcc atccatcgtt ggaagcatca catctaaaat gatgaaatca aatgattgtt
     5041 ctaatgctaa ttctaatcct tcgcctccat ctgtggcact ggttaccttg tagccttctt
     5101 tttccaaatt gaacgtcaag agcgttacaa tagaaggttc atcatcaaca actagtattt
     5161 ttttcattca aatctccttt aaataggttt cattctgttt atattttagc atagaatcag
     5221 tacaaagggc tattttacaa aagaaataaa tggtataata ggaaggcact ttgctgaata
     5281 gaaaggaaga gcagtcaatg attggaatca gtgcatgttt aggcggtctg gcctgtcgct
     5341 atgatggaca gaaaaaagaa gtaccagaat tgatggaact agttgaaact ggagaagctg
     5401 tcatggtctg tccagaagtg atcggcggat taccaattcc tagagatccc gctgaaatca
     5461 ttggtggaaa tggattcgat gtgtggaaag atcaagcaaa agtatggacg acaagcggcg
     5521 aagatgtaac ggaagcgtac aaaaatggcg cgatcaaagc ctatcaaaaa ttgcaggaaa
     5581 aacacattga tctagtcatc atgaaggaaa aaagcccttc atgcggaacc cattcgattt
     5641 atgacggaag tttttccgga ataaaaaaaa atggtgccgg agtggctact gcttatttta
     5701 ttcagcaaaa aatgactgtg ctctctgatg aagaatggct gaaaactaga ggtgagcaga
     5761 atggaaatcg aaaagaccaa tcgaatgaac gcgctctttg aattttattc gaccttattg
     5821 acagaaaaac aaatgaacta catggaatta tattatgcag atgatttttc attaggtgag
     5881 atcgcggaag aatacgaagt aagccgacaa gcagtatatg acaatatcaa gcggacaagc
     5941 aagatcttgg aggattatga aaaaaaactc catctttttt ctaattatat tgttcgagaa
     6001 caggtattgg aaaatatgac tgcatatatt acagataagt acccggaaga taaaaaacta
     6061 cttgaatatg tacagcaaat tcaagcaatc gaagaataaa taaaaggaat gaagaaaata
     6121 tggcatttga aagcttaact gaacgtctcc agcaggcgat gggaaaaata agaaaaaaag
     6181 gaaaagtatc agaagctgac gtaaaagaaa tgatgcggga aatccgcctt gccttactag
     6241 aagccgatgt taacttgcaa gtagtcaaag attttactaa gcgcgtacgc gaacgtgcgg
     6301 taggggcaga agtgctggaa agtttatcac ctgcacaaca aatcgtaaaa atcgttgatg
     6361 aagaattgac gatcacactt ggttctgaaa cagcagagtt aaataaatca ccaaaaatac
     6421 caactgttat catgatggct ggtcttcaag gggcaggtaa aacgaccttt actggtaaat
     6481 tagccaatta tttaaagaaa aatgaaaatg ctcgtccgct cctaatcgca ggggacgtat
     6541 atcgaccagc tgcgatcgac caattgaaag tattaggtca acagctagac gttcctgtat
     6601 ttgatatggg aactgaagtc agcccggtag agatcgtccg tcaagggatg gagttagcaa
     6661 aagaaaagaa aaacgactat gtattgatcg atacggcagg acgtctgcat atcgatgaaa
     6721 cattgatgga tgagttgaaa caaatcaaag aattgacgca accaaatgag attttgcttg
     6781 ttgtcgatgc catgactggt caagatgccg tcaacgtagc tgatagcttc aaccagcaat
     6841 taggtattac aggggtcgtc atcactaaac ttgatgggga tactcgaggc ggggcagcac
     6901 tatcgatccg ctcagtgaca ggcgcaccaa tcaaattcat cggttctggt gaaaaattga
     6961 ctgacttaga agtattccac ccagatcgta tggctagccg tatccttggt atgggggata
     7021 tgctgacatt gattgaaaaa gcgcagcaag actacgacga gaaaaaagcg gaagaacttg
     7081 cacagaaaat gcgggaaaat tcattcgatt tcaatgactt tattgaacag cttgatcaag
     7141 tgatgggtat gggcccactg gaagatctaa tcaaaatgat cccaggaatg aatcaagtcc
     7201 ctggaatcga aaacgtcaag gtagatccaa aagatgttga acgcaaaaaa gcgatggttt
     7261 attctatgac gccagcagaa agagcaaatc ctgatctgtt gaacccaagc cgtcgtcgcc
     7321 gaattgctgc cggttctgga aacagtgttg ttgaagtgaa tcgaatgatc aaacaattca
     7381 aggaatcaag aaaaatgatg cagcaaatgt ccaaagggga tatgaacatc ccaggtatgg
     7441 accaaatgtt tggttcaggc gtcaaaggaa aactaggcaa aatggcgatg aatcgaatga
     7501 tcaagaaaaa taagaagaaa aagaaaaaac ggaaataaga tgaaaaaaac agcttcggta
     7561 cagactgaaa atgtctactg ccggagcttt tctatcagca atttttattc tttactaata
     7621 caattactct aaacgaagag gtggcacaga tgagatgtcc gtggagtgaa caaacagaaa
     7681 gtatgcggca atatcatgat actgtctggg gtgttcctga atatgatgac caaaagctgt
     7741 tccgaaaatt gatgcttgat attaatcaag ccggattgag ctggcagacg attttaaata
     7801 aaagtgaagc attcgatgaa gcatatgatc gattcgatat caaaaaagtc gcaaattatg
     7861 gagaaacaaa aatcagtgaa ctaatggaaa acaaaggaat tattcgtaac cgaagaaaaa
     7921 tcgaagcagc cgtccaaaac gccaaagcag tcttgaaaat tcaagaagaa atcggcagtt
     7981 tttcagagta tctttggtcg ttttctaatg gaaaaatcgt tgattcttct tatcattcac
     8041 aagaagaagt cccaacaaca agtcctcttt cagatgaatt agcaaaagat ctaaagaaaa
     8101 gaggatttaa atttatcgga agcacaacaa tctatgcttt tttagaagct gtcgggatca
     8161 tcaatgatca ccttgacagt tgtttccgca aagaagaggt cgtacgtatc ggcagaaagg
     8221 gcgaataaag catgcgaaaa atcatatttt atggtgctat aagtttagat ggctatttgg
     8281 caacgaaaac ggatgatctt caatggcttt ttgacactcc aacaggtaag caaactactt
     8341 atgaagcttt ttatgaaaca atcgatacga ccatcatggg cagaaaaact tatcaggaag
     8401 caaaaaaata tttggagacc gaagcaattt atccagagaa aaagaatttc gttttttcca
     8461 cccacgcaca attacaattg ccagatgctg aggtcgtaaa cgaagatcct gttgcttttc
     8521 taaaaaatct acaaatgaca gaaggaaaag atatctgggt tgtgggtggc ggtcgattga
     8581 tcaaaccgct attggaacat caaatgattg atgaatggta tatccagatt acccctgtat
     8641 tattagggga cgggattcct ttattccaaa aaggaacata tgaagcgcgt tttgaaatgc
     8701 tagacacgac acgattcggt gaatttatcg aattgcatta tgtacgaaag tagaagtaaa
     8761 taaaataaaa aaggcaacta tatgcagaaa tgtcctgtac atagttgcct tttttattac
     8821 cgttatgctt tttactctgt taatttgtta gaaaaatcta gatacgcatc taaaatcgtt
     8881 tggaagaaac ttaatgttcc ttcaacgata ttaccatttt catcaattag attcgcaacg
     8941 tttccgatat atgcttccgg ttgctgcaag gttggcacat tcaaaaagac aagtgattgg
     9001 cgtagatggt ggtttgcgcc aaagccgccg atcccacctg gtgatgcagt aacgactaaa
     9061 cctggtttat gatcccaaac actgtgtcca taaggtcttg agcctacatc taaagcattt
     9121 ttcaatactg ctggcacaga acggttgtat tcaggagtaa cgaaaacgac accgtctagt
     9181 cccctcactt cctcacgaaa acgagtccat tcagcaggag cctgttctgg agtttcgaga
     9241 tcctcattgt aaaatggcag atcatcgatt tttacaaata cactttcgta cccttcagga
     9301 agcaatttcg caaaagcttc tgctactttt ttgttgtaag aatcttttct taaactacca
     9361 ataaaaaatc caattttttt tgtcatacga tttttcctcc ttcaaactat ctataataat
     9421 cttacaaaag ataagtaact ggagcaaata aaaacactta ctgacaaaag ctacatagaa
     9481 gatagattac ttatttttaa caaaaaagaa gtctaaaatc ttctattttc taaaaaaaat
     9541 cgaatttaat caagctgtaa agaaaaaaag ctttacaggg taaaaaaaaa ctgttacact
     9601 acatcttgtg aataaaacaa tggaggtgga aataaataat ggcagttaaa atccgtttaa
     9661 aacgtatggg ttctaaaaag agtccgtttt accgtatcgt cgtagctgat tcacgttctc
     9721 ctcgtgatgg acgtttcatc gaaactgtag gtacttacaa tcctttgaaa gaccctgcag
     9781 aagtagtttt aaaagaagac ttagttcttg actggttatc aaaaggtgca caaccttctg
     9841 acactgtacg taacatcctt tcaaaagaag gcgttatgca aaaacaccac gaagctaaat
     9901 tctcaaagaa ataaggtgac cggatatgaa agatttaagc gacttagtct taacaatcgt
     9961 tcgtccgcta gtcacttatc ctgatcaagt caagttagac gtcgtagagt cgaaagactt
    10021 ttacgagtac aatctaacag tggctcctga agatattggc cgtatcattg gaaaacaagg
    10081 gcgtgttgct aaagcaatcc gcacaatcgt ttacggtgtt cggatcaatg caccgaaaaa
    10141 agtgcgtttg aatatcatcg atgataagga aaagtaaata aacgtgctgt actcgaattt
    10201 tcgagtgcag cttttttgtt ttttttatta attgaagaaa tgattatttg gcaaaacaag
    10261 caagaaagcg tatccttata ggaggaggga gtgaacagcc aatgagaaag aaaatgttat
    10321 ttattccatt tgctctattg ttaagcgctt gtagttcggt tactcaagaa tcagacaata
    10381 cacatctatc aagcagtata tctgaaactg ttaccagtcc agaaaaaagt agtaccaaaa
    10441 cggcttccac cactgaacaa acaatggcta cctcaaataa tgagaaaaag actgctttag
    10501 accagttgaa agaacaacag ccaaatgttc ccatgcctct agatgtacca gtctcttcag
    10561 gctatctcaa tatcgcagcg acacatacaa agcaaggtta ttctatttta tattacagaa
    10621 cagatcgtcc cctcgggttg aatgctgacg aattgaatca agaaacgcct attgcaacct
    10681 atctatatca atatgggttt gcttctagtc aagaaacaat ccaagttttg cagccatttg
    10741 agatcgacac aaatggtcag caagtggact taggttctcg aatcacaggg tatcagcaag
    10801 gtgcagcagg ttcaagtttt cttgaatggc aagaaggaaa ctggcgtatt cgaatccgtg
    10861 ggaataatat agaaggccaa gatcccttac tattagctaa agaaatcgta gcttatttgg
    10921 aagaaaacag tctaccagca cctgaacaat ttggtaaaat tactgttgat atgggtgata
    10981 cgacgaatcg agccgtagaa gtaagctggc aagaaccgaa aaatgcctat acgatcactc
    11041 atcaagatcc tatgtctgcg ttgaagatgg cagtttctat gaagaggttg tgaaatcagc
    11101 gcgctgatct gagcccatca tttaactcca agtcacagga agtaatcaaa aatagcacgc
    11161 cgcatttttg cataattttg gcttaatacc ttaaaagaaa actagaattg cctcttcaca
    11221 catgctaaaa taaaacagac aaaaaagaaa tggggatata agttgactga atacttgaat
    11281 gtaggaaaaa tcgtaaacac acaaggtatc aaaggcgaag tccgtgttat ctcaacaacg
    11341 gatttcccag aagaacgtta caaaaaagga acggtgctaa cactattcca agacggaaaa
    11401 gcacctgtgg aattaacagt caaaagtcat cgcaaacata aaaattttga cttattgagt
    11461 tttgaaggac atccttctat caatgatgtg gaaaaatatc gggatggcat attaaaagtg
    11521 tcaaaagaca actctgtaga tctagctgaa aatgaattct attaccatga gatcatcggt
    11581 ctgcatgtat tagaagatgg aactgagtta ggtaaagtaa aagaaatctt gtcaccaggt
    11641 gccaatgatg tttgggtcgt tcaacgtccg aaaaaagctg atatattgct tccatatatc
    11701 gcttctgtcg tcaaagaagt cgatttagaa gctggagtgg tccgtgtaga aattccagaa
    11761 ggactgattg acgatgaaga ttgatgtatt gactttattt ccaagaatgt ttgaaggtcc
    11821 tatgggggaa tccatcatcg gtaaggctgt agataaagga ttattagata tcaatatctc
    11881 taacttccgc gattattcag ataacaaaca tcagacagta gatgattatc catacggcgg
    11941 tggagcaggt atgctgctca aagtccagcc catctatgat aacatccaag ccattgaaaa
    12001 agctgcaccg gatgtcaaaa aaagagtaat tcttcttgac ccagctggta aacgattcga
    12061 tcagaaaatg gcagaagaat tttcacaaga agaacactta gtattcattt gtggtcatta
    12121 tgagggatat gatgaacgta tccgcacatt agtgacagat gaagtttcct taggagatta
    12181 tgtccttact gggggagaat taggtgcgat ggtcatgatc gatgcaacag ttcggctttt
    12241 gccagatgtg ttagggaatc agacttcagc tcaaacggat tcttattcaa ccggtttatt
    12301 ggaacatccc caatatactc gcccggcaga atataaaggg atgaaagtac cagaagtatt
    12361 gacaaatgga aatcataaat tgattgaaga atggcaatta aaagaatcac taagaaggac
    12421 ctatcaacgt cggcctgatc ttttggaaac acttgaatgg acacctcaaa tgacgaaatt
    12481 tttagaagaa atcaagaaag aagaacaaga aaaaacagca gatgaagcca agtgactttt
    12541 tctaaaatct tctttacagt aggttgataa tatgttagta ttttatggtg agtgcaaaag
    12601 cacttaacta ttacgatatt ccgctgtgag aaaagctcat aagaatattt ggaataagga
    12661 gaagaaaacc atgaatccat taatcgaaga attaacaaaa gaacaattac gttctgacat
    12721 cccagcgttc cgccctggtg acactgtgcg tgttcacgca aaagttgttg aaggtactcg
    12781 tgaacgtatc cagttatttg aaggtgttgt tatcaaacgc cgcggtgctg gaatcagcga
    12841 aacttataca gtacgtaaag tttctaacgg agtgggcgtt gaacgtacat tcccattgca
    12901 tacaccacgt gttgcaaaaa tcgaagtagt tcgctacggt aaagtacgtc gtgcaaaatt
    12961 gtactaccta cgtgcattac acggaaaagc tgctcgtatc aaagaaatcc gtcgttaatc
    13021 attgttatca gactgagaaa gaacccttgg tattcaaggg ttcttttttt attatctgtt
    13081 tttttatatt aacgatttct atttggtaca tttttgatta ataaaaaggg gcaaaaaagg
    13141 ggcaacaagt tacagttttt ctatatcttc tttcaatttt tcttttactc cttttgtcac
    13201 atgcaaataa atcttttcag tgatatcact attttcgtgc ccaacgcgat cctgaatagc
    13261 atacagagga gttcctagtt ccgctaattt tgaaatatgg gtatgtctaa aaatatgtga
    13321 actaagtttt ttatcaatct tcatgtcagc tttatgattt ctcaaatagg tgttaattgc
    13381 tgttagttgg aaaggagttc ctttcgttgt ttgaaataga aattgaccct tcggatttaa
    13441 ttctagaagc tcattgtaaa tagctatcgc tttttttggt aaatcaattt ctcgcattcc
    13501 agcagcagtt tttgtagaat cagatttttt catatcagct attgaacgtt ctctatacat
    13561 catcgtccca tttataacta ctgatgcatt attattggta atgtgtacat cgtctttact
    13621 taatgcgatt gcttctccag gtctcattcc agtcaaatat agccactgga aaaggagaga
    13681 atatctttta ttgtgtgaag ttgtgaaatc tactagtctt ttatattcat catcttctaa
    13741 aaacttatct ttaattttta tcgtttttga ctctcgttta taatcaataa ctacttcgtt
    13801 gataggattt ttttctacat agcctttttt catagcgtat gaaaaaagaa gattcaattt
    13861 ggatttaatt acactgacgt acttatttga caagtcatct ttatatatca tatcttcaaa
    13921 aatattattt aaatctactg tattaatacc agaaacgatg taagtctcag gcatcttctt
    13981 ctttatagtg tttaagatat tattcgtagg ataatatgta gactccttaa cttgcctttt
    14041 gtaaataact aaccattctt cgacaagctc atgaaatgtt agattaggct tctggagtgt
    14101 tttttcattt agttttatat caattttttt atttaactcc agtaatgcca ctttctgagt
    14161 ttctcgagac ttatttttat aagtaatgct cacttttttt ctttttctag tttttggatc
    14221 aatatatcgt tcattatatt tatatacttt ttttccgttt ttatcagttt tggtttcaat
    14281 ccacattgtt ttcatctcct atttgttgct ataataggaa tggataagta agccgattat
    14341 agcaggttta tttttcatca cgtccacaaa ctttggcgag ggagggggcg tgtttttgta
    14401 ttttaacgat ttatattgaa tgttgattca gcactttcag caactgtact ataatcagac
    14461 ggattatata acactccggt ttttatcgtt acttttgtaa tatcgttaac tttagatgga
    14521 tctaagtaag ctaaactata aaattcttta ctttcagttt ttttgaaaaa cgagtttgtt
    14581 gtcattgtat gcataagatc gtctgaactg aaatcaattt gttggccgtt atcaagtatg
    14641 gcgtgtgcaa gcccattata accataatct acttcagaat tgtttttaac tttgaatgca
    14701 atttgtaaac cataaaaatt tccttcacta tctttgtgat tatttgcatc gtagtaacca
    14761 gcagcgctag ggtcagcata attggaatac ttgagtagtt tgatattaga tatataaatg
    14821 ccgatatcac caagaggaat atctgattgt gggcttgccg ttccaattaa ttcaattttg
    14881 ccacttgtat ctcttttttc tttggtccat tgaccaactt tgaggagggg gccatcaacc
    14941 tcctgtgaat ttttagaaga ctgtgttgta ctttctgata acgtaactga agagtctttt
    15001 tcgtttgttt tgccagtatt atctacaaca ttagattggc tacatccggt aagaaaaaaa
    15061 gcaccaaata ctaaccctaa aatcactttt ttcattcttt attctccgtt tctatgatat
    15121 tatttttatg taggatctta gaaacgagat cttagtccgt gttgcagcac gggctttttt
    15181 ctttataact tttttagaga ttataggcaa aatagtagga cacaaaaata tattattgta
    15241 ttccgtattt agaaaatcct aattgaactt caccagaagt tttttgctgt gttgtacgca
    15301 atgcttcttc ttcagacatt ccattctgta ctttccatgc aacaggcgac atcccgtatt
    15361 tgttaacaaa atcagtaagt gataaagtgt cagcgtcttg ctgaccgctt gtttgttggt
    15421 cttctggatt ttgttgagat gctgcttgtt gttctttctg atcttgactg ataatattgc
    15481 cagcatcatc tgtagtcaat ccattttcat aaagggccac gccgaaagct tcccactctt
    15541 tgttggacca atttgcacga tcagctggag ttgactgtaa agtgcgttgt ttcatctgtt
    15601 catatgtttc ttcttgaggt gcggtttgga ttgtatcctg actggagctt ataactgttg
    15661 ggctaggttc cgccgtagct tggttagagc ttgtaactgt tgaactagaa tctgtcttag
    15721 atgtagattt gctagtagag gaactggttt cagttgtttc ttttgtttta cttacttttg
    15781 tttcttggtt agaagtggca tctgttgatt cagctttttt attatttgaa caagctgaaa
    15841 gtagcagagc agtacttaac aacaacataa cgctaacttt tttcatttta taattcctct
    15901 ttctcgttgt aatatgtgtg ctaacacggg cttttttata taagaaaacg ataagcgctt
    15961 tctggaagtc cgtaaagatt ctttaattca tcgatttttt tgggatattg atcattatct
    16021 tctttataaa gagaaacaat gagattagca gcaaagcaat tagcttcgct ttcagatttg
    16081 cttctagatg ttcttgttga tacatagtaa ctggataagc cgcgatgaaa aatagcgtga
    16141 cctaattcgt gagcgcaaat gtagaatctt tcctcagagt ctcgcagttc atcatttaag
    16201 aagattattg cacgacctct aatttcttga aactgtcctt tgggattttc gataaaagga
    16261 acgtattgaa ttttaatgcc catcttttca caaatataaa aaggattagc agaccggtat
    16321 ttccgcttca actcctcgac taaattaatc gtatccatct ccataagctc acatcttttt
    16381 gcctttttct ttgtcttctt tcacaatatc ccagaaggtt ccaataagga tatcttttac
    16441 gcgttggacc tgttcaggcg tcagtgtttc tccgccataa gacatattga catttgaatc
    16501 tagtaattta tcaagttcaa ctacttcatc ttttgttgcc cagtcaggaa cttggtttct
    16561 accgagtaaa taatcagtag ttacattaaa ataatctgct acaagttgta gtcttttagt
    16621 actaggagtt ctatttttcc attgataaat cgtgttttta ggtatattta attcttcttc
    16681 caattgagtt atacttatgc ctcttttatg agctaattcc tttattctat ctagtaaatt
    16741 cattttcgca cctcacaagc tacgaaaaca ctaataaaaa aattagttaa aggtgttgac
    16801 aactaataaa attgttagta tactgttttc gtaagctaaa atattagcta aaaagacttg
    16861 ataactaata aacacttcac ggtcggcaaa ctctgaaatg taaattacta ggcatttctg
    16921 tgtcttattt agctatatct atatactaat aaaaatatta gttattgtca acgaatatta
    16981 gctaattttt tagcttacag actatttttt agaaaggagc tatttttatg tctgagaatt
    17041 tagacttaaa aattcgagcg gagatgagaa aaagaagaat gactttcaaa gaactagctg
    17101 cgcttgttgg tatttcagga gcttatttat cagatattct aaacggcaat cgtgatggaa
    17161 agaaagcaca acagcatatc gaaacagtga aaaaaatatt ggacatccga tagggggggt
    17221 aggcgatgac aaaactaaaa aaacaagatt ttgtaaaaaa atacaattat tctccatcta
    17281 cttatcaacg tcgaatgtcg gaactaaaaa atacagcaat tttctcagcg gcgtatgaac
    17341 gggtcacagg acaagaagtt tggatcaata cagaattata cgataaattt ttgtctttca
    17401 aatcctataa caggttacgc acaagaaaga taacacctaa agaatttatc gagaagcatt
    17461 tagttgattt ataaaaaaca gaacattttg agaggtgaag gttaatgggc aaattcaaca
    17521 gagcgctagt gttcagcgca ccgctaatca tctacgcttt aggcctctgg ggaagcaggc
    17581 aagcgttgat aggaacgatt gtttacatgg tttggatttt tatggggctt gatgaagctg
    17641 aagctgagta cagagcgaaa aagccaaccg agggggctga ctaaaaatga atagaaaaga
    17701 gaaactagaa tgtatattac tattactcag tttaattctt tcactaattt ctctattggg
    17761 aagtttgtat ttttgatatc aacaattttg tggatttggg ttctatttag agtaaatatc
    17821 atttttgttt ggtgattaag gagtttatta aaattaattt tccctacttc aaatgcaagg
    17881 taacaagata tagcactttt aggaggaata tttagcggta cagcttcaga gtatattcta
    17941 ttatcatcga ctgatcctcc tttagcaatt aatttcttaa atggagtagc tgaccatctg
    18001 cggtttaagt tagcggatga aagttctaaa ttaactaaca tagctggttc tgttgaatag
    18061 ttagaaatga ttacttttgt ataaatcata tctgcagcga agtaagaagc attaagttca
    18121 acttctagtt gagggcgcat tccttttatt tttatccctg ttaaaaacgt actcaatata
    18181 aaacttatta ttgacatcca ttgaaaaatt gttaagtgaa aaaaattcaa aataaacacc
    18241 accagttttt aactaaatta taccaaaaag gagagaagaa ataatgcaag aattagtaat
    18301 tttgaaaaat aaagaagctg tgactacgag cttacaagtg gcagaaa
//
LOCUS       DBNICI010000043        18273 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.043, whole genome shotgun sequence.
ACCESSION   DBNICI010000043 DBNICI010000000
VERSION     DBNICI010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.043"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(68..1960)
                     /locus_tag="AC1ZCC_001220"
     CDS             complement(68..1960)
                     /locus_tag="AC1ZCC_001220"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1629484.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(1957..3684)
                     /locus_tag="AC1ZCC_001221"
     CDS             complement(1957..3684)
                     /locus_tag="AC1ZCC_001221"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1629485.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3700..4350)
                     /locus_tag="AC1ZCC_001222"
     CDS             complement(3700..4350)
                     /locus_tag="AC1ZCC_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIQ1629486.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4554..4850
                     /locus_tag="AC1ZCC_001223"
     CDS             4554..4850
                     /locus_tag="AC1ZCC_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIQ1629487.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5042..5662
                     /locus_tag="AC1ZCC_001224"
     CDS             5042..5662
                     /locus_tag="AC1ZCC_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HIQ1629488.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5754..6920)
                     /locus_tag="AC1ZCC_001225"
     CDS             complement(5754..6920)
                     /locus_tag="AC1ZCC_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIQ1629489.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(6932..7285)
                     /locus_tag="AC1ZCC_001226"
     CDS             complement(6932..7285)
                     /locus_tag="AC1ZCC_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HIQ1629490.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7413..8435
                     /locus_tag="AC1ZCC_001227"
     CDS             7413..8435
                     /locus_tag="AC1ZCC_001227"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HIQ1629491.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8454..8615
                     /locus_tag="AC1ZCC_001228"
     CDS             8454..8615
                     /locus_tag="AC1ZCC_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629492.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(8874..9308)
                     /locus_tag="AC1ZCC_001229"
     CDS             complement(8874..9308)
                     /locus_tag="AC1ZCC_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629493.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9418..11277)
                     /locus_tag="AC1ZCC_001230"
     CDS             complement(9418..11277)
                     /locus_tag="AC1ZCC_001230"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIQ1629494.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11278..11418
                     /locus_tag="AC1ZCC_001231"
     CDS             11278..11418
                     /locus_tag="AC1ZCC_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HIQ1629495.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11395..12555)
                     /locus_tag="AC1ZCC_001232"
     CDS             complement(11395..12555)
                     /locus_tag="AC1ZCC_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIQ1629496.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(12862..14286)
                     /gene="araA"
                     /locus_tag="AC1ZCC_001233"
     CDS             complement(12862..14286)
                     /gene="araA"
                     /locus_tag="AC1ZCC_001233"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HIQ1629497.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14302..15000)
                     /locus_tag="AC1ZCC_001234"
     CDS             complement(14302..15000)
                     /locus_tag="AC1ZCC_001234"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HIQ1629498.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(14957..16618)
                     /locus_tag="AC1ZCC_001235"
     CDS             complement(14957..16618)
                     /locus_tag="AC1ZCC_001235"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HIQ1629499.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16836..18236
                     /locus_tag="AC1ZCC_001236"
     CDS             16836..18236
                     /locus_tag="AC1ZCC_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HIQ1629500.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
BASE COUNT         5211 a         3859 c         3292 g         5911 t
ORIGIN      
        1 tcggaaaaag ggactgtgac acgacttttg tcacagcccc taaaaaaaat aataaatttg
       61 tactattcta tgaagcttct tcttcagaaa actgactgtt ataaagatct gcatagaatc
      121 cgtctttttc catcaatgta tcgtggtttc ctgtttctac gatcgaacca tgattcatca
      181 caataatgtt gtctgcatcg cgtattgtag acaagcggtg agcaacaacg aaacttgtcc
      241 ggttttccaa taggcggttc atcgcttttt gaatcagtat ttctgttcgt gtatctacac
      301 tagatgttgc ttcatcaagg atcaggacat ctggatttgc taagaatgcc cgcgcaattg
      361 tgattagctg acgctgccct tgcgaaatat tgcttgcatc ctcgtttaat acagtgttat
      421 atccttctgg caatttacga acaaaatcgt ctacgtgagc agctttagca gcctcaatga
      481 tttgttcgtc tgttgcatcg tcgttcccat atttgatatt gtcatagata cttcctgtaa
      541 acagccaagt atcttgcagt accattgaaa actgtgcgcg caattcatca cgtgaaagat
      601 cacgggtatc tacaccatcg taacgaatac tacctccaga aacatcgtag aaccgttcaa
      661 gaaggttgat cagtgtcgtc ttccctgcac cagtcggacc tacaatagcg accatctcac
      721 caggtttgac atccaagtta aaatcagtca ttagcaagtt atctggtgtg tagccgaatt
      781 gaacattttc aaaacgaact ttgtatggtg tatctttttc ttctggaagg ccagattttt
      841 cattggacat ctcttcttca tccaacactt caaatacccg ttcggctgaa gcaactgttg
      901 attggatagt gttcatcaaa cttgcaagtt gagtgattgg ttgagagaac tgtgttgtat
      961 attgcaagaa cgcttgaaca tcccctaatg tcatgttccc atttgcaact tttactccgc
     1021 ctagtacagc gacgaacaca tagcctagat ttttaacgaa attcatcaac ggcatgatga
     1081 ttgccgaaat aaattgagct ttccaaccag cggcatacag attctgattt tctttttcaa
     1141 atacttcctg gtctttttca gtatgattga atgttttaac aatggtatga ccaccatatg
     1201 tttcttctac ttggttattc aataaaccta aacttttttg ctgatctgcg aaaaacactt
     1261 gtgatttcgg cgctacgatc atcacgatga tcaagcttaa agggatcatc aataacgcga
     1321 ctaacgtcaa ttgccagctg attgaaagca tcatcacgat gaccccaatc aatgtgactg
     1381 cactagttac aaattgagtc aaattttgct gcagcgtgct tgcgatgtta tccatatcat
     1441 tgattgctcg agacatgata tccccatttg tatgtgtatc ataataggaa atcggtacac
     1501 gattcatttt ggcttccaaa tcatgacgta attcataaac tgttcgttgg gaaacacgtg
     1561 tcataataaa ttgctgtaaa aagttgaata cagcagaaat caaatacatc acgattactg
     1621 tcgcaatgat ctgcgcgatt ttatcaaagt ctatcggaaa ttttgttact tgttggcctt
     1681 gttgcatctg ttgagcgcca gccattacac ctttgaatat ttctgtggtt gctttcccca
     1741 agacctttgg tgtttggatt tggaagatca cggctgctat tgctagtaca aatacagcga
     1801 tgatcgcaac catacgttta gacatatagc caaacaatcg gcgtaccgtt ccccagaaat
     1861 ttttaggctt ttcacctttt acaccgagat ttctgccagg tccaccatgt cctggaccgc
     1921 ctgctggtcc atgagaggga cgtgtgtttg ttttactcat gctaagtcct cctctcttag
     1981 ttgtgaatga acgatttctt gataagtttc gtttgttgct aatagttctt catgtgttcc
     2041 tttgccaacc agttttcctt catctagcac aaggatcata tctgcatcca ttactgtact
     2101 tactcgttga gcaactaaaa cggtgatact ttctttcata ttcgttttca atgcttggcg
     2161 aagattggca tccgttttga aatccaacgc tgagaaggaa tcatcaaaca catagatatc
     2221 tgctgttttg actaatgccc gtgcaattgc cagacgctgt ctttgtccac cagagaagtt
     2281 tcctcctcct tgttccacat ggctatctaa gccatcttcc atctctgaaa caaaatcttt
     2341 agcttgtgcg atttctaatg ctttccaaat catttcatct gttgcatccg gcttaccata
     2401 ctgcatattg tctcggatcg ttccagagaa cagaactgct ttttgaggaa caaagcccat
     2461 taattctcgc aaattatatt gagtcatgtc actaatgtcc gttccattta tttggatcga
     2521 accagattcg atgtcatata atcgagggat caaattgact aaggtcgttt tacctgaacc
     2581 agtcccccct atgattgcga cgatctcgcc cgacttggct tggaaatcaa tatcttccaa
     2641 tgcaagtttt tctgctccat gatatcgata attgacatga ttgaaggcca atgttgcgtt
     2701 ttttccagca aaagaaactg ttttaggatt ttcagggtca tggataccga ttttttcatc
     2761 caacacttca ttaatacggt cagctgaagc ttgtgcacga ggcaccatga taaaaatagc
     2821 ggaaagcatc ataaagctga tcaagatctg catcgcataa gtcataaatg caatcagatt
     2881 accgacttcc aaagtcatat ctgctatgta gtgtccacca aaccaagtga ttgctacgtt
     2941 tgtaccgctc atgatcaatg tcatcattgg catcatcaat gcgacgatcg tattaacttt
     3001 gattgccgta tttgtataat ctttattcgc taaatcaaaa cgattttctt caaagcgtgt
     3061 cttatcgaat gctcgaatca ctctgacccc tgtcaatcct tctcgaaata ccaggtttaa
     3121 acggtcagtt ttcttttgca tacttttgaa taatggaaca gcaaagtaca tgattccgcc
     3181 gatcaaaata atcattactg ggatgacaac taagaagatt tttgtcaact gatgatcttt
     3241 ttgataagca agtatgctcg cgccaatcaa cgtgattggt gcattgatca tcatacgtaa
     3301 aaacatttgt gtaaccattt ggatttgagt cacatcattg gtcgttcttg tgatcaaaga
     3361 ggccgttcca tatttatcaa acgcatcttt tgtcagactc tctgattttt tatagacatc
     3421 cgtacgcaat tgcttaccca gtttctgtga ttcacgagta gcaaaaaatg tatttgcgat
     3481 agcagctaag atactgatca aagaaaagcc gatcatcaca aaacctgtat gccaaatata
     3541 atcgacatct ccttgcgcta cccctttatc aataatattg gaggtaagtg tcggcaaata
     3601 taaatctgcc agtacttgga tcaccatgaa aactgcagct gcaattgctg aagtcaagga
     3661 gatccgtttt acgattttta gcatctagtt cccttctttc tatttatata ctccgttttt
     3721 cagccaagaa agtttcaaat tgaattcact gattgcttgc tctactgagt aatcttcaga
     3781 tgttgaaaat aagcgatacg aatgcgcaat tgttgtaaat acagtattca tcactaattg
     3841 gatcagtaat tccaattcat gatcattttt tactttcagc ttcgtccgat cgatcaattc
     3901 aagcaactct tttgcatctt gtttgtgttc tttatgtgga tcgtgaggcg ttttttggcg
     3961 actcgtatga ggagcgactt ttcttgaggc acggtaatcc atgttcataa acaagttacg
     4021 ataaaaagaa gcattctcgc cttccaaaat ctcataaatc attttagaaa aataggattc
     4081 gaatcctttg aatagatcac catcggattc tttcattagt tgtaacatat ctttagagct
     4141 gtcacgtctc attgtctcaa agtaataaaa ataaagatcc tctttatctt caaaatactg
     4201 atagaaactg cctctaggga tatccgctaa tttgacgata ttagcaatcg aagcttcttt
     4261 taagggtact ctagaaaact cgattctcgc agcctcgatt agtctttttt gtttttcttt
     4321 aggcaaatgg aaaaatgtct gtgtcggcat atggattctc ctttctttca agatgacaca
     4381 ttgtcatatg acgctatgtc attataacac ctcctacctg ttttgcaaga catttgcata
     4441 gtttttttca ttatcatccg atacattaaa aaagtgataa gaggaatcct tccatttgaa
     4501 ttaatgagag ttgcccatta aaatagccac atactaaaca aaggatgaga taaatggccc
     4561 aatcaattag tgtccaagac ttcaataaat tgcctctcga tgctgacagc atcgttttag
     4621 atacaagaaa taccttagat tacacaaaaa accacttaag ccactcgctt tctatccccg
     4681 ctgctatctt gccaagacaa ttaaaaaatc tagatccaaa gaaactttat tatattctaa
     4741 gctatacagg caggcgatca gaagcattag ccgatcaact tactgctaat ggttttcgtg
     4801 ctgtttatat cattggtggg atggaagctc ttcagtcgag tgttgcttga atattgcttg
     4861 ttagattcaa aagaggctgt gacaaaaaat cgtgtcacag cctcttttat accctgtctt
     4921 tttttagtta tttattcttc aaccttatca atatgacttg atagcctttt ctttttcgag
     4981 taaactgatt ttgttagtca tagttataac aatcatagca ataaaaggag aaggtaagca
     5041 tatggcatct gttttagtag tcaaaggtca tcccctaact gcagaagaat cgcgcacagt
     5101 taaggcattg acgtcttttt taacaagtta taaagaaaat catccagatg atgaagtgac
     5161 tgttttggaa ctttatcgcg atgatattcc ggaaatcgat gaagaattat tatcaggttg
     5221 ggaagcatta cgtgctggtg cagaattcac ttccttatca gaaagtcaac aacaaaaaat
     5281 tgctcgtttc aatgaattga ctgatcagtt ccttgctgct gataaaatcg ttatcgcaaa
     5341 cgccttatgg aatctaaata tcccaacaaa attaaaagct tggttcgata ccgtcaatgt
     5401 agctggaaaa acgttccgct atacagaaaa cgggccagaa ggattagtga caggtaaaaa
     5461 agcactgcac atccaatcta atggcggtgt atacaacgga caagactttg cctctcaata
     5521 tgtaaaaggt attttaaact ttgtaggcat cgatcaagta gatcagctat tcatcgaagg
     5581 aatcgattat gaccctgacc gtgcagatga gttgatgcaa aatgcattag ataaagctgc
     5641 tgctttaggt aaatcatttt aaaaattgag ataaatgcat acaaaagaga cagaggaaaa
     5701 aatcttcaaa gcagcatttg tgccttggat attttcccat ctgtctcttt ttgttattct
     5761 ttctgctttt tcaacggatt ttcttcataa agagaagaga ttgttgtgta ccattcaaaa
     5821 acatgagtaa tcactacttt agctgctgca taaacaggaa tccccaagat cacaccgatg
     5881 attccgaaca atttcccaga agtcagcaga actaataaaa tcgttactgg atggattgct
     5941 aactgattcc ctagtactaa tggagagatc aagcgtcctt ctattgtctg ctctactgca
     6001 aaaacgatca gtactttgat gatcatgaat ggacctgcta caatggctaa aaagatagcc
     6061 ggaatcatcg ctaaaaatga tcctaagtaa gggatcaggt ttaaaaaacc cgcaataatc
     6121 cccaacgtga cagcatactc taaaccaatg accgcaaaac caatcataaa catgattgcc
     6181 acagcaaaag ccacagttag ttgtcctctt atataagaag aaacttgact attcatttca
     6241 ctcagcacat tcaacgtcgg tttttgcatg cgtgtcggta aaaatttgac aaaataaggt
     6301 gctaattgtt taccgtcttt caatagataa aataagataa acggcatcgt cagcagagcc
     6361 actaaaatag tcgctaccgc gccgacaaaa cttccaagac tctgaactgt actcttagat
     6421 atatcttgga tcatatcgcc tgccgaattc atgaacttat cacctgctgt ttccaactgt
     6481 tcgcgaaatt ggttgaacag cgggtcccgc aaaatctcgg ttaatttatt atcaatcgta
     6541 tctacatatt gagggaagtt actgatgaag ctcactgttt gttcctgaat cttagggatg
     6601 atcactacgc tcccccatgc gattagtgca acaaccaaaa caaacagccc aatgatactg
     6661 taaagccttg gtacttttcg cttttcaaag taatcaacta ccggattcat caagtaatac
     6721 aagattcctg ctaaaatgat aggcagacct acgattgcta aaaattgcca gattggtgca
     6781 aacaaatatg aaacttttgt aaataaaaag acgatcagca atattaacaa tacaacaagt
     6841 aaggctgtga ctacttgatt atttaaaaac cagcgccaaa accacgatac cggtctgttc
     6901 ttcttatttt ttccttccat aattcctcct gttagttata aaaaatttgg cttcccaaac
     6961 caatagcagg cagtctatgc ggatgaagat agattccatt tgcaatttgt ccttcttctg
     7021 gacaagcatc aaaaatcaat gatacgtgag ctactgtctt taaattccca ttggccatcg
     7081 gaccgacata atcgatcgta tatacctgat catcaaaagc aattgttccg cctgctttca
     7141 aagaaaaagg ttcgtctttt gatacttctt gaatcaccga atattttcgt aaggcagctg
     7201 ttgctgtttt atcgaacaaa atcagcatgg gttcttcctt gtttattgct tctgtcccaa
     7261 tttctgtaat ctttgcctga atcattgcac tctctccttt aattgctttc aaaaacagta
     7321 taacacattc tcattcttta gccgtgtgga ataattatgt gatagaataa aaacgaaaac
     7381 tatttcatta caattaggag gaatcagctc atatgatcga aaagataatt ttaggaacct
     7441 atacgcgtcg cgaaagtaaa ggaatctata cgatcgacct tgatacagaa aaagaagaat
     7501 tagccaacct ttcattagct gttgaagaaa atagccctac ctatgtagca aaaagtaaag
     7561 ctggcaatct ttacacagtt actagtgtgg atggattagg tggtgccggt gcgtacgata
     7621 aggattttca ttttttgaat gcggtaacag agtctggcgc tcctttgtgt tatgtagcgg
     7681 ttgatgaagc acgtcaatta ctatacgggg cgaactatca taaaggtgaa gtaaatgtct
     7741 accatatatt aggagatggc gaactgaaag cagcagattc aatattccat caagaagcga
     7801 caggtcctca taaaaaccaa gatcatgcgc atgtccacta tacggatctg actcctgacc
     7861 aacgattagt cgtatgtgat cttggtacag atcgtgtgta tacttatgat gtgtccgaaa
     7921 acggcaaatt gaatcttgta actaccttca ctgcagagcc aggaacaggc cctcgccatc
     7981 ttgtctttca tccaaatgga caattcgctt atttatttgg tgaattagac agcacagtcc
     8041 gcgtgctttc ttataataaa gaaacaggtg cttttgaaga attacagaaa atatcgacct
     8101 tgccagaaga attcgacggt gaaaatggag gggcagctat ccgcatttca aatgatggca
     8161 agttccttta tgcttctaac cgaggacata actcgattgc tgtattcgca gtttttgaag
     8221 atggttccca cattcaaaac aagcaaatca tctcaacaga aggggatttt ccccgcgatt
     8281 tcgcactaga tccaagcaat aattttgtcg tgtgtgccaa tcagaacacc gataatctga
     8341 cattatatcg ccgtcatcct gaaacaggat tactggaatt actgaaaaaa gatgtctatg
     8401 taccagaatg tgtctgtgtt tattttgatc gttagtaaga aaaggagaat gaaatgaatc
     8461 agcaagacgt attagacaga ctacgggaag aactaaatat tcctttcttt gatgggaaaa
     8521 tcgaagataa agagtatagc gaagaggaat atcagaaact caaagccgat ttacagaact
     8581 actttgaaca atatgtacag aatgtagaaa actaatcagc tttccatact atctgaaaag
     8641 aacatcagga caagactttt ccgtctatcc tgatgttctt ttctacaaat aaaaaaccgc
     8701 cgagatggtt agactcgacg gaaaaggagt atttactcga ttttgagtaa gtacaaggtt
     8761 gttgttggtt ataggtatgt cattatcata aaggattttg atcactctat cataaaggta
     8821 atattacatt tatattagat aactctagtt tttattgtta tcaaagattt cttttatttt
     8881 ttcaacaaca aattagtcat tttttggatg gcagctgtca attggtctgt cgcttggtct
     8941 tgttttgctt cagatgtatt gaatagatct tcatctaccc ctgattcaag atcgatactt
     9001 tggatcattt cttcacagcc tgctacatat gaaagataag ctttttcaaa atttttatga
     9061 atgcccattg cttttgctgg tggacgcaaa gtactgactt tcataagcat tgcccgatat
     9121 ttttttgttc cttcttcaaa taacaggatg atctcttcaa gagtcgattt gtccagttca
     9181 cttgtttttc cttcatcaat tgcgtttctg atcacttcat atttcggatg cattccttct
     9241 ccgattgctt ctgtttcctt gacgatttga tcgatggact gcacataata gccaatattc
     9301 ggtaacatgt tcgttttcct actttctcca ttgttgttat tatttattct acttgttttt
     9361 atcctcaaat gacaagtact tgaaaaaatt taatggtttc tatacagctc gccattctta
     9421 ttcgtgctca aagaaaccat ctgctaaact atattgtttc cctgcgacaa tgtcatattg
     9481 gatcaattga tattcgtcgt aaatctcttg ttgcgagcga ttgaattgcg cttctttata
     9541 ccactgtccg ttttgatagt acagttctcg ttcaaacgcc ggaagttcct gttctaatga
     9601 taaaagcagc tgataaaagc catttgttgt ctgattcgtc tgttccatta gattcgctgc
     9661 aaaataaaac ggactggtga ttgcatcgtg agtcgtttgc ttttctaatt ctcctttact
     9721 gtcgaacata agaaattgtg tttcatgcaa tgtttgtttg tcattttttg cttggatcgt
     9781 atctgaatag atccctggaa gatgatcccc ccaaaaaaca actaaagtcc ttctagaaag
     9841 attcttcaat tcttccgtga actgcttcag tgccgtgctg ctataggaaa tatcttgcag
     9901 ataattctct aagtcaagtg tcccacttcc atctgaagtt tttgcaggat agtctagatt
     9961 atcatacttt ccatcgtagg gcatatgtgt ctgcatcgtg actaaatgaa tgaactgagg
    10021 tgttttttct tccttgagaa gtgtcattac ttctttgtaa gctgactcat cagaaatata
    10081 tggattattc tctatcgtat ccgtataggt catcgtacgc tcgctgatga actggtcaaa
    10141 tcctaatgtc tgataaacat cctctctttt atacatcgaa gtattgtatg gatggatcgc
    10201 tgtcgtctgg tatgattgtg cgtttaacgc tgaaacgata gaaggaagct gatccatttt
    10261 tggtacaagc atcgtgtaag gggtggtgag ttgcgcatta aataacgcca tagaaaaacc
    10321 agtcaatgct tcaaattcga tatttgctgt tccaccgcca tagttttgag aaagcatatt
    10381 gccactatat gtttgatcag ctacttcata ataatctgcc aatggctcac cagatacttc
    10441 tacgccgttt aatcgactag gatctgagaa actttcactc atcacgaaca cgatgttcgg
    10501 cgactcttcc tctacagttt tatttttttc atcggctagc ttctggtatt tttcagtgat
    10561 ttctttgatc ttttctttcg aatatccttc aggttcatcc attggttcta ccttcagatt
    10621 gtacaaaaac ccaccgataa aaccagtgtt atagtagttc attttctggc tgtaaggtat
    10681 ccatagtgcc gttttattat atgcttttcg caacaaatta tcgggattgt tgaaattact
    10741 gatataccct agcagtccaa ttgccacaag cagtgtcgtt aatcggatca attggaatcg
    10801 ccttcctttt ttcaggcttc gaaacatgta ccagcaaaac aatcctatca cggttcctgc
    10861 ggcagctaag atcactgtaa acagcatagt gccggtcata tctttcagca atccgatttc
    10921 agtgatcatc ttcaaatcgt ctggataaat cggttcttgc cgataactca ttttcatata
    10981 atttgcaaaa cctaatacac cgatggctac gacatacgta agggaaccta aagggacaga
    11041 tcctgctagt gcaatcagaa aaataagaat aatcagcaag acgagacagg ctaagaagaa
    11101 tttttccgta tgccaagaaa aagcaaattt caacgcaagg tctacggata gatcattttg
    11161 acaccactgc aaaaataaat tactaaaaac aacaagaaaa aagatactga tgattgctgc
    11221 tgttcctttt aaaatctttt tctgtttggt cgaaagtctg cttatatatc ttttcaaatg
    11281 cttgctcctt catttctatc aataaaagaa tgttgcttct cttattttag ttgtacgaaa
    11341 ttcgaaagta aagaacggaa agaagaatta atgaaatcca aatattctct ttctttatgg
    11401 cctttctggt ttctttaata agcggacctt tcgtactcca gaaatttttt ctagctgttg
    11461 tttcacttga ttgagctgtt caaaatcagt actgtctatg tcaatcaata tgtagacatg
    11521 ctcattctta tggttacgcg atatatctgc gatcgctagc tcgttctcat tcagcagaga
    11581 aaatatctct gctaatatat tggttttctt ttgataaaat atagtgaatc gaaacggcgc
    11641 ttggaaaaac attcgtgcgg atggataatt gaccgcttct ctaaccgtac cgaacaaaag
    11701 ataatctttt aaggcttcaa ctgccaaacg tccgctgtct gccaaagctt cctgagtcga
    11761 tccccctaga tgaggcaaca taagtatacg gtcattttct aagaactctt cttctggaaa
    11821 atcagtcaag tatttagcca gatgttgttt ttccaatgct tgcataacag catatttatc
    11881 aacaatttca cttcttccta cattgataag tacagcatct tttttcattt tttctatatt
    11941 cgcctgatcg atcaatcctt ttgtatcttc tgtcaacggc agcatgacta cgatgtaatc
    12001 cgaggtagaa agaactgttt ctaaatctgc ttgccgaaca taatccaact gggcatcgcg
    12061 gatagaataa cccaagacat ccatccctaa gctatagcaa cttagcgcta cttttgtgcc
    12121 tatcgctcca agaccaagaa gaccgatggt tttgccttgc aattctcgtc ccacataagc
    12181 acttcttttg ttttctgcct gttctaaaat attaggacct gttagtgttt gtaccatacg
    12241 acttgcttcg attatcggac gggaagataa gagcaaacag cacaaaacaa gctcttttac
    12301 tgcattagca tttacacctg gtgtattcat gacgatcgtc ccgttttcag aagctttttc
    12361 aacgttgatc gtgttcaccc caacaccagc tcttgaaatt gctagcaaat ctggtgtaag
    12421 ccattcatct ttcactttcg tactacgaat aactattcct tcggctggca aatcatctat
    12481 tattttgaaa tattcccctt gtaacttttc tatctcttgt tctggaatag catcaatcaa
    12541 atgaacgtga tacataaaac cccctcctta ctaacaagta tctcgaaatt tctgcaatta
    12601 gacaactgtt taccatttga cttcctagaa gcaaaaagag cagttagagc agagtaggct
    12661 gagtgataaa aataaggcta tgacaagtta ctgccataag ccttattttc gagagcaaaa
    12721 tattgagggt gagatatctt gataaatggt gttccgttag ctgacattga taataagact
    12781 aaatttcaaa aaagttgaaa agcaaatttc gtcaatctct tcttatttct taaaactaag
    12841 tggctttttc acaacctctt tttatctaat aaccactgtt tccaactcaa ctaattttgc
    12901 ccacgtttca atttgatctg ttgttagatt tagcgagaca acggtatgat gaccgccacc
    12961 tgcctgaatc catgaatgga caccttcatg gaaattcggt ttagttttcc ataacacacg
    13021 tgcaacaggt aaattaggtg cttcagtcgt tggttcaaag gcttctactt cattgatcag
    13081 cagtttataa tgcgtaccga aatctgccat cgatacgact accccttctc ctgctttccc
    13141 atcaaataca agacgcgccg gatcttctct atctcccatt gataaaggag aaacaacgat
    13201 tcttggtttc gtcgcagcaa gcgtcggatc tacttccatc atatgggatt ccaagattgc
    13261 ttcttggcca ctagctagct cgtaagtata atcttccatg aatcctgtat ctttccctcg
    13321 agccatgatt ttcagcaacc gatcaatcgc tgcagttttc cagtcgcctt ccccagcaaa
    13381 accgtatcct tctgccatca aacgttgtac agctagtcca ggtagttgtt tcattccata
    13441 caggtcttca aaattagtcg taaaagcgtt atagcctcct tcttctaaga aacggcgaat
    13501 accgatttcc tgctgagctt gtacttttac atgttcctcc catgtctttt cttcataatc
    13561 accatagtcg aaggtataca attccttgta ctcttcaaat aattgatcga cttcttcgtc
    13621 tgatacgcga tcgattactt gtacaagatc tccgatacca aaataatcaa cagtccaacc
    13681 aaattgaatc tgtgcttcca ctttgtctcc ttctgtgaca cccacgtttc tcatgttgtc
    13741 accaaaacga gccactttta ttccaaaact ttcattatag gctactgcaa catccatcca
    13801 atcagcaatc tctttttgga tctcaggtcg tttccagtag ccaacgacga ttttattttg
    13861 tttatttaat cgagcattga tgaacccata ttcgcggtca ccatgagcgg attgatttaa
    13921 attcatgaaa tccatatcga tcgttttcca aggaatgctt tcgttatatt gcgtagctaa
    13981 atgaagcaat ggtttttgta gaagctttgt cccgcggatc cacatttttg ctggtgagaa
    14041 agtatgcatc caagtgatca cacctgctac ttcttctcga taattgacct ctttcatgat
    14101 ttttgtgatc gtatcagcag taactgctaa ttcttgaagg cggattggat aaggcaattg
    14161 gccattttca ttcagttcat cgaccatttc ttgtgcatgt ttctttactt ctcgcagtgc
    14221 ttcttcccca tataaatgct gtgatcctac aacaaaccaa aattcttttt ctccaatatt
    14281 caacataatt agccactcct tttatttgtt ttgtccgtaa tacgcatttt ttccgtgttt
    14341 tcgtaaataa tgtttatcta aaatccgttg aggcagctct tctgaaaatg aattgagctg
    14401 tctggtaaat aaattcatct tgcatacttc atctagtacg actgcgttca ttaccgcact
    14461 ttgcgcatct ttcccccaag taaatggacc gtgtccgtgt aaaagtaccc ctggaattgc
    14521 taagggatca attttgcgtt ctttgaatgt ttctatgata acttttcctg tttcatattc
    14581 gtacccgctg tcgatttcct gttgtgtcaa aaatcgagca caaggtaccg atccataaaa
    14641 agtatctgca tgcgtcgtcc ccatcgcagg aacatccaat cctgcttgtg cccagatcgt
    14701 cgcccatgtc gagtgagtat ggacgattcc tccgatttga ggaaatgaac gatataaaac
    14761 agcatgagta ggcgtatccg aagagggatt gagtttacct tcaatgatat gattgtccaa
    14821 gtccacaacg accatgtcgt ctgctgtcat ctcttcataa ctgactccgc taggtttgat
    14881 aacgaaatat cgtgtttcag gatcaaaagc gctcacattt ccccatgtat atttgacgag
    14941 tccgtgttta ggcaagtcaa gatttgcttg gtatacttct tctttcagtt gttccagcat
    15001 tttctatcac tcctttttct tgcaattgat gaatcgctgt tttttcaatg tcgatcccct
    15061 cacggtaacg tctcataaat atctgatagc ctgctatttc ttctgctgaa ggtgtgatgg
    15121 tgattccttg gtcttgtcca aacacgtctt ctgccaagaa gttttctaaa gacttcccat
    15181 gtcgatccat catataggct gctaataacg cgattcccca cgctccacct tctccggctg
    15241 tgtccatcac tgtaactggt gcctccatag cagatgctag aatccgctgt ccaacttctg
    15301 gtgttttaaa aatcccccca tgccccacta aacgatcaat ttggacattc tcagatttca
    15361 atatatccat tccgattctc attgcaccaa aagcactggc taaatggatc cgcatgaaat
    15421 tcgctaaatc aaaacggcta cctggtgtac gtacaaataa tggccttcct tcatttactc
    15481 ctgtaatgtt ttcaccggaa aaataaccat aagaaagtaa tcccccgcaa tccgtatcgc
    15541 cttctagcgc tttgttgaat aataccgtga ataatttatc tctgcttatc tccataccca
    15601 aagaattggt aaattcttca aaaatactga cccaagcatt gatatccgaa gagcagttat
    15661 tcgtatgaac catgcctact agttctcctg aaggtgtagt gaccatgtcg atttccggat
    15721 ggacattttc tagttctttt tctaatacga tcatagcgaa ggcggaagta ccagccgaaa
    15781 tgttccctgt ccctttggct acactgttcg tagcgaccat accggttcct gcatcccctt
    15841 ctggcggaca aaccgggatt cctggtctca aatttcccgt agggtcgatc agataagccc
    15901 ctgattcgct taacttgcca gcttcttctc ctgctactag aactttaggc aaaatgtgat
    15961 ccaagtcaat cgctacgtgc gctttttcga ttaactgacc aaaaatattt ttcatttctt
    16021 gatggtaggt tttcgttgaa ggatcgatag gaaacatgcc tgatgcatct cctacgccta
    16081 gtactttctc accagtaagc tgccaatgga cataacctgc aagcgttgtc aaaaaattaa
    16141 tttctttcac atgttcttct ttattcaaaa tagattgata caaatgagcg atactccatc
    16201 tctgcggaat attgtattga aatttttccg ttaatattcg ttctgcttct tcagtgattg
    16261 tatttcgcca agtacgaaat ggtactagta actgttcctt gctatcaaat gctagataac
    16321 cgtgcatcat tgcactgaac ccaatcgacc cgattgtcgt aagattctcg ccatagattt
    16381 cgaatacttc tgctgccatt tttcggtaac tgacgcgcag tcctttccaa atatcttcta
    16441 atgaataggt ccagatacca ttttctagtt tgttttccca ctcataacta ccagcggcaa
    16501 taggagtaaa agatgaatca atcaatacag ctttgatcct agttgatcca aactcaatcc
    16561 ctaaagatgt acgaccatct atgacatcta acatactttg cgcatttact tccttcataa
    16621 tgatttaact ccctccttct cgtcaaacct gatcaaaccg tctttttagt agcgcttaca
    16681 atgattattg tatacttttg tacgtacatg tcaagcagtt atttacaaga atacaataaa
    16741 aaacacttgt tttgtacgta cataagttat aaaatgaaat tgcttacaaa atctgatcaa
    16801 ccgccttctc gaaattctag aaggagagaa tacttatgaa aaaagaaaaa aaagtttcct
    16861 catcgtttat ttattttttt ggcgcatttg gcggcattct atttggatat gatattggtg
    16921 ttatgaccgg cgccttacct tttttacagc atgattgggg tttggcaggt aaagcttctt
    16981 tgataggatg gatcacgtct tcggtcatgt taggtgcgat ccttggcggc tctttatctg
    17041 gtcttctttc tgataagctt ggacgacgaa aaatgattct tctttctgca cttattttta
    17101 tggctggttc tgtcctttct gcatctgccc ctcacaatgg ttcttatttt ctgattgcag
    17161 caaggattct tctaggttta gctgttggcg ctgcatctgc cctagttcct gcttatatgt
    17221 ctgaaatggc tcctgctaga ctaaggggaa gattgtcagg gatcaatcaa gtgatgattg
    17281 cttctggcat gctcctttcc tatgttgcag attatttgct aaaagggttg cctgaaacga
    17341 tggcttggcg cgtgatgtta gggctcgctg ctgtccccgc gctgatttta tttttcggtg
    17401 ttttagctct accagagtca ccgcgttttc ttatgcaatc tggccgcttg gaagaggcaa
    17461 aaagagtatt gaactatatt cggacaccaa aagaagctga acaggaattc gaacaaatcc
    17521 agctgaatgt caaacaagaa aagacaaccg gaacttcttg gcatactttg tttttagaaa
    17581 aatatcgttc cttagtcatt gccggtattg gtgtagcagt attccagcaa tttcaaggag
    17641 ctaatgctat tttttactat attccgctga tcgtagaaaa agcgactggg catgcagcga
    17701 gcgatgcatt gatgtggccg atcatccaag gaatcatttt agttgctggt tctttgctct
    17761 ttttggttat tgcagacaag ttcaatcggc gaactttatt gaaaataggc ggtagcgtga
    17821 tgggactttc tttcatccta ccagctgttt taggaactgt actggatgct catacaaatt
    17881 cgttgttgat tctcttgttt ttatgtattt atgtcgcatt ttattcttgc acatgggctc
    17941 cactgacatg ggtaatcgta ggcgaaatat tcccactagc tgtccgcggg cgagcatctg
    18001 gcctagcctc ttcttttaat tggattggtt ctttcctagt tggcttgctt tttcctgtga
    18061 tgactgcttc tatgtctcaa gaaatcgttt tcggcatttt tgggatcatc tgctttttag
    18121 gggttttatt tattcaagag atcgtaccag aaactcgtgg gaaatcttta gaggaaatcg
    18181 aacaaagtgc atctaaaaaa acttatccta aacgtatttc tatccatcat tcttaaagag
    18241 gttgtgacag aagtggtcag cttcaagaaa taa
//
LOCUS       DBNICI010000044        18129 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.044, whole genome shotgun sequence.
ACCESSION   DBNICI010000044 DBNICI010000000
VERSION     DBNICI010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.044"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..189
                     /locus_tag="AC1ZCC_001237"
     CDS             <1..189
                     /locus_tag="AC1ZCC_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629501.1"
                     /translation="VRYVGIRDLLSDNCCSKYKVNQVREALNKDNNVERIKNVFGYSI
                     EEINYYIDFAEAFIPMVR"
     gene            292..606
                     /locus_tag="AC1ZCC_001238"
     CDS             292..606
                     /locus_tag="AC1ZCC_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HIQ1629502.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            584..2278
                     /locus_tag="AC1ZCC_001239"
     CDS             584..2278
                     /locus_tag="AC1ZCC_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HIQ1629503.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            2298..3476
                     /locus_tag="AC1ZCC_001240"
     CDS             2298..3476
                     /locus_tag="AC1ZCC_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HIQ1629504.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            3439..4125
                     /locus_tag="AC1ZCC_001241"
     CDS             3439..4125
                     /locus_tag="AC1ZCC_001241"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HIQ1629505.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            4125..5285
                     /locus_tag="AC1ZCC_001242"
     CDS             4125..5285
                     /locus_tag="AC1ZCC_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HIQ1629506.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            5295..6170
                     /locus_tag="AC1ZCC_001243"
     CDS             5295..6170
                     /locus_tag="AC1ZCC_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629507.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIKIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            6167..6478
                     /locus_tag="AC1ZCC_001244"
     CDS             6167..6478
                     /locus_tag="AC1ZCC_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629508.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            6468..6821
                     /locus_tag="AC1ZCC_001245"
     CDS             6468..6821
                     /locus_tag="AC1ZCC_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HIQ1629509.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            6811..7212
                     /locus_tag="AC1ZCC_001246"
     CDS             6811..7212
                     /locus_tag="AC1ZCC_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629510.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            7205..7609
                     /locus_tag="AC1ZCC_001247"
     CDS             7205..7609
                     /locus_tag="AC1ZCC_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629511.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            7621..8226
                     /locus_tag="AC1ZCC_001248"
     CDS             7621..8226
                     /locus_tag="AC1ZCC_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HIQ1629512.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            8246..8608
                     /locus_tag="AC1ZCC_001249"
     CDS             8246..8608
                     /locus_tag="AC1ZCC_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629513.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            8611..8793
                     /locus_tag="AC1ZCC_001250"
     CDS             8611..8793
                     /locus_tag="AC1ZCC_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629514.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            8810..12229
                     /locus_tag="AC1ZCC_001251"
     CDS             8810..12229
                     /locus_tag="AC1ZCC_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HIQ1629515.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            12280..13017
                     /locus_tag="AC1ZCC_001252"
     CDS             12280..13017
                     /locus_tag="AC1ZCC_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HIQ1629516.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            13027..15318
                     /locus_tag="AC1ZCC_001253"
     CDS             13027..15318
                     /locus_tag="AC1ZCC_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HIQ1629517.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITASQAWQNAKTLAKPVIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            15342..17423
                     /locus_tag="AC1ZCC_001254"
     CDS             15342..17423
                     /locus_tag="AC1ZCC_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629518.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGNYDYSGNPNLMSKLKSSDFN
                     VGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFVD
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDGPYWLGKVPLGENIADHTVKFPIESGDH
                     QIYQGYTEEELMIGQTYTITLKGTKPASQTFVAYNHWTARLGELKPVDGLTDVWSLTF
                     TPTNVVASSPKLFRVYQYPQSTVGACRIDWLKIEKGDTRTPNISEYKYRGTGMRDSNN
                     PKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKD
                     AQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALN
                     GKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYG
                     SDYTFAITYTI"
     gene            17440..17589
                     /locus_tag="AC1ZCC_001255"
     CDS             17440..17589
                     /locus_tag="AC1ZCC_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629519.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            17586..18032
                     /locus_tag="AC1ZCC_001256"
     CDS             17586..18032
                     /locus_tag="AC1ZCC_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629520.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            18034..>18129
                     /locus_tag="AC1ZCC_001257"
     CDS             18034..>18129
                     /locus_tag="AC1ZCC_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HIQ1629521.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEE"
BASE COUNT         6414 a         2721 c         3803 g         5191 t
ORIGIN      
        1 gttcgctatg taggtattag ggatctgtta tcagataact gttgtagtaa atacaaagtg
       61 aatcaagtaa gagaagcgtt gaataaagat aataacgtcg aaagaataaa aaatgttttt
      121 ggatatagta ttgaagagat taattattac attgacttcg ctgaagcttt cattccgatg
      181 gtgagataac ccccccttaa aataaatcgc aaattttttg ggggtgatga aacggagggg
      241 gctgtcagga aaagagattt tttcgaactt tatcatgaaa ggagggctaa aatgtttaaa
      301 aacgaattgt ctcaaaatcg ctacagagaa aaattacgcc gctctttaat aagccaattg
      361 gaaagtcaga aaacaaatat tgagccattc ttagataatg ttgatcgtta tatcagttta
      421 tgggaaacgg cgatatcact ggaagaagat atatccgaga acggcattag actggagaat
      481 ggtaaaaaga atgaatcagt agcgttgctt gtttctgtca acaaacaaat gggattgatg
      541 ttggataaac ttgccattac tcctgaattg gtaggtgaag caaatgaatc aattcctgag
      601 ttataagcat attgaaaatt ggttcaaagc tatagaagaa ggcactatca aggtatgcaa
      661 agagcaatta ttgctaaaaa attatctaga agaaagagtc tttactagag aagatattta
      721 cttcgataag cagatggtag aggattcaat caatatacca gcacaatact ttccattcga
      781 attaattccg tgggaaaaat ttctacaatg ttttatttat ggtgttcgat ggaaaaaaga
      841 taaaacacta gtgttcaata gatatctttc attaatggga cgtggtaatg gtaaaactgg
      901 ttttgcttct tggaacaact tctttctact aaccgctaaa cacggtatta aaaattatga
      961 tattgatatc tatgccaata atgaaagcca agcaaagact agttttgatg atgtatttaa
     1021 agtaattaaa gatcatcctg atttagataa aaaagtattt aaagctacga aggaagttat
     1081 tcaaaatatc gctacaaaca gcaaacttcg ttataacacg gcaaatgcta gaacaaaaga
     1141 tgggaagcga ccaggtgcaa accgctttga tgaaattcac gaaaatgaag attattcaat
     1201 gataaatgtg gctacttctg gtggtggtaa aattcgagat tatagagaat tttatgatac
     1261 aactaatggt catgttcgtg gtggtccgct tgatgacatt atagaagaat caaaaatgat
     1321 tctttctgga gaacttggaa ttgacaagga tggagcagaa ttttctagtt tgtttccatt
     1381 tatttgtcgc ttggataacg ataatgaagt tgatgatccc gacatgtggg aaaaagcttg
     1441 tccaactatt aattacaatg cagatttaaa acggaaaatg tttcaagaat actctcaaat
     1501 gcaacgtaat gctggtttaa gacttacgtt catgaccaaa cgaatgaaca gacctatgga
     1561 agatacacga tttgctgttg cttcatatga tgatgttctg catacgaaag aaaaagaatt
     1621 tcctgaaaaa atggatgaag tgataggaac agtcgatttt gctgatagac gagattttgc
     1681 cagcgttggg ttgctaggaa aatacgataa agatgtttat tttacacaac atacttttat
     1741 ccacgaatca gcccttcgat tacaaaacat caaacgagag gttatagata tttctataga
     1801 tcaaggaaaa tcacagatcg ttcatggaaa aaatatagaa gctgattata ttgtaggttg
     1861 gtttcttgaa atgagtaata aatattatat taaaaaaatc gctatggata tgtaccgtgc
     1921 aaaaatattg aagcccgctt tagaagaagc aggttttact gtggaaattg ttcgaagcgg
     1981 atctgttaca catggtatgt taaaagatct ggttgatgac ctttttatta atcaacgttt
     2041 attttttggt gacgatgcga ttatgcgttg gtattgcatg aatgtatatg aagagcatat
     2101 ttctaatgga aatatacgct atgaaaaaat agaacctgaa actagaaaaa cggatggctt
     2161 tttttcattc cttcatggtt tgaatttttt agatgatatt tatgattctg ctcctgtaac
     2221 agtcacaaat agctcagtag aaaatacagg aactggattt actcctctag tattctaact
     2281 tgaaaggagg tgagaaagtg gggatttttc aaaaggcggt aggatacttc acaaaaaaag
     2341 caacggttcc tttagaagaa tacttttgta aattgcaagt tgattttgtg tatcgaaaat
     2401 ttgcgattga aacttgtatt gatttgattg caaatgcgat gagtaaagcg gaattcaagt
     2461 catatgaaga tggaaaaaat aaaaagaatg atctttacta taggctgaat gtagctccta
     2521 ataagaaaaa taatgcaaca gaatttagaa aaaaactgat caggagatta atattctaca
     2581 atgaagtatt gatcgtttct ccgtctaata attctagcga aatatttatt gcggatagtt
     2641 gggatgtcac agaatatgca ttgaaagatg atgtgttttc tcaagtgcaa attaacaaca
     2701 tagtccttga tagagaattt ctagaaagtg atgttatcta tataaaatac gcagatcaac
     2761 aaattaggca actagtcgat gcgtattatc aagcgtatgg gaaactcatt tctagtgcca
     2821 tgaatgttta caagcgctct aacgctcgta gatacgtact gaaagggaat ttattccgac
     2881 cgcaagacaa tacaacacaa gatcaaatca ataaaatgat gacatcacaa tttaaggctt
     2941 ttatggaagc tgataatgca ggtgcggtat ttcaattaca aaatgagtac acattagaag
     3001 atttcagcgg aaacttccaa agcaattcaa gagatataaa aaacttaata gacgacatct
     3061 ttgagatgac agcagcagcg tttcacgttc cgaaaaacct actaaaggga gacatgagtg
     3121 ggttatcgga tcaagtggac gcttttttaa tgttcgaaat cataccgatt gctgaactta
     3181 ttcaggatgc gtttaacgct agtctctatg aagtagaaga atacttgtca gggaattttg
     3241 tacgtgtgga tacaactatg atcaagatta ctagcttcaa agatttggtt gacgctattg
     3301 atgtaggcat tagaaatgga gtatttacaa tcaacgaagg aagagagcgc gttggaaatg
     3361 atcgctctga taaggcgatg gcagatgaaa tatttataac taaaaacaat caacaagtat
     3421 cgaaaggagg tgaggcgaat gacgacaatg aaaacatttc tagcagtaaa gaatgaaggc
     3481 gcagtaccgc aaatttttat tcagggattt attggttcta gttggttctt tgaagggaat
     3541 actgacaagg gaatcaaaaa tattttggat agtctaggtg atcaagaaga aattgaagta
     3601 gtaattaatt caaacggtgg agacgtattt caagggattg ctattgggaa cttacttaag
     3661 tcaaataaag caaaagttaa cgttgtgatt aacggcttag ccgctagtgc tgcttcaatt
     3721 atcgcaatgg ctggcgatac tataaaaatc tacaacaatg cacaattgat gattcaccgc
     3781 gcttccacat acggagaagg aaatgtcgat gacttccgta cgattgctga ccaactggaa
     3841 tcaattgata aatcggtaaa ggcttcatat aaaacacgat tcaatggcac agatgaagca
     3901 ttgcaagaac ttcttgaaaa agaatcgttt atggatgcag aaacagcttt gagttatgga
     3961 ttggtcgatg aaattatcga tgcagaaaat agctcaggta ctgaagctaa aaaagaacaa
     4021 agcgttgaag aaattttgaa tgacgttaaa gaaaaaagag cagaaaaaat tgctgcattt
     4081 acagcagcat taaataaaac atttggacaa ggagatgtaa aataatgaca gttaaaaatt
     4141 taaaaggtgt aacagctgca agcgaccaat tgatgaaagc ttttaaagat ggtaacgaag
     4201 aatcttttag cgcagctatg gtaagcttat ctaaggaaat tcaggataaa attttagaag
     4261 aagcaacagc aaaaaatcaa gatcaattag tattaatgaa ccgtggtcag cgtgtattaa
     4321 ctacacaaga aacaaaattc tataacgaag tagtgaaaaa cgaaggtttt gcaggggtcg
     4381 aagaattagt gccagctact gtatttgaac gtgtatttga agatttagaa caatctcatc
     4441 cactattgca aaaaattact tttgttaaca caactggtgt aacagaatgg attgtgtcac
     4501 gtggagtcaa tccagcatgg tggggtaaac tttgcgaagc tgttaaaaaa gttttagata
     4561 atggctttga tgtaattaac atgaagcagt tcaagctatc aggttatatt cctgtatgta
     4621 aggcaatgct tgatttaggt ccagtatggt tagatcgtta tgtccgtact gttttagtag
     4681 aatcattgag aattgcatta gaacaagcaa ttgttgatgg tactggtaaa gatatgccag
     4741 tcggaatgat gcgtgacatg agcaaacaaa ctagcggaga atatgctgaa aaaaaagcag
     4801 aacctattac agctttagat gctgtaacta tgggcggttt gatggcgcga ctatcaaaat
     4861 tcaatatcga aggtgtgaat gatccgattt atcgtaatgt gaatccttct gatgtggtcc
     4921 taattgtgaa tccaactgat tactggtcta aagtattccc agctaagact gtactaactg
     4981 ctaatggaga atacgtacaa gtattgccag taccagtttc agatttgcag tcaacggctg
     5041 tgccagaagg aaaagcagtt attggggtag cctcagatta cttcatgggt gtaggatcta
     5101 cactaaaaat tgaagcttca gatgaatacc attttgttga agacgaacgc atttatctag
     5161 ctaaacaata tgcaaacggt caacctaaac gtaacgatag tttcattgta ttagatatta
     5221 gcgctttggg aactactact acaactacaa aaccaacaac cacaacaact acaacacaag
     5281 cgtaggtgat cagaatgaag tatattcttt gtcagccggc aatcaatcgg tttaaatggg
     5341 agcttgaagt ttgtttaact aatctgaaga aactaggaat caaagatatc gtattgcttt
     5401 tcagcagaca cgatgatcag attcctattt tttttgagaa ggaatatggt gttgaagttc
     5461 atgtgtacga tgatctgcgg gacgacaaag agtatattcc ttcgattaaa ccatatttat
     5521 ggtggaaata tttagaagaa gatcattcgc gtgaggacga ccgatatttc tatatcgatt
     5581 cggatgtcat tttcaataaa agaattaatt tgcgcaaatt gccttctaaa gatgatgttt
     5641 ggtattgtag cgactgctgt agttatctaa gtcttgatta tattagaagc tgtgaaaacg
     5701 gagaaaatat tctaaaagat atggcaaaca ttgtaaatgt tacagtagaa tctttggaaa
     5761 ctataaacac taattcagga ggcgcacagt gggttattaa ccgccctaaa gcgaattatt
     5821 ggaaaaaggt ttatctggat tctaatcggc tatatcgcta ccttagaggg caaaaaacaa
     5881 atataaaaat ctggacagcc gagatgtggg cacagctttg gaacatgatg tatttcaata
     5941 ttggtcctaa agttcacgag gaattagact tttgttttgc tactgatcca atagaaaaag
     6001 ttaaagaagt aaaaatctta cacaatgctg gagtaacaac aaacgatgaa gatttatttt
     6061 tcaaagggag atacgtgact tctacgcctt ttgatgaaga tttatcattt gtaaacaaga
     6121 aaaaatgctc ttacgcatat gttaaagcaa ttaaggcggt ggttagatga cgcctgaaca
     6181 agtgactgaa gaattgctaa tagctgtgaa ggataatatt tacgttacct ggaacgaaga
     6241 agatgagtca attaaaaaga tgatagctaa aaatgctgtt tatcttcaaa gtaaagtgag
     6301 tacaacactt tctttttctc ctgaaagctt agaatacgga ttgctaatcg aaagatgtag
     6361 atacgactgg aatcgtgctt tagatgagtt tgaacaaaat ttcgctagtg agttattagg
     6421 tttcattcaa cattatgcgc tacaagaata tattgcaggt gatgtgaatg gcgaataatc
     6481 gtagactcga agaaacattc aacgatggtt ggttaaagat tttgacgcaa actaccaaaa
     6541 gaaatgaact aggaaaaaag attggtgtag aagatacaga aatcacttct ttaaaattta
     6601 gaaatctttc catgagagat agtgatataa cagctatgga tgcgatggga tcgaaattaa
     6661 ctaagaaagt aaagactcca tttcatccaa tcgccaagaa atttaataaa gatcaatatt
     6721 ttatcgtaat cgatagtatg cgttacaacg ttatctatgc cgattacgat aatttttata
     6781 tctattttta tcttgaaagt gtgggtgaat atggtgattg ataattctaa agaaaaagaa
     6841 cgtttaaata agcaaatttc tgctatcaaa acttccttag aagagcattt tggcctcaaa
     6901 ctctttcaag actccgtagg cgaggatgag ctacctgatg attttaatta cttcattctc
     6961 gaaacaggag aaatagaaat gatcactgag ccaaaatata gcgtgggtca aaatctatat
     7021 ctaactttct attcagaaaa tagagaagat ttaacaggag attcactaga tattatttca
     7081 ttgattcaaa atcgttcgat tcgttttcag agaatggatc ccaatcattt aaaactagag
     7141 aaccaagatc gctatatcga tcaattggta tttacgttta gacgattatt gaagagtgat
     7201 tgtcatggct aaaaatagtt gggagctaaa aataaatgga catgatgaac ttcttgtgcg
     7261 gatggaacgc tattcaagcg agagcgaacg actgattaac gaagcattga aatcaaaagg
     7321 ttcggctatt gcagtggata ggattacaga aaaaattcct gtttctgaag cagatttaag
     7381 aagagggcac caacacgcaa aaaatagtcg tccacttaag actcaataca ttaatttggg
     7441 tttcatcatt agacctacaa gaaaatttga gtatttaaaa tatcctgatt tggggatagg
     7501 tacttctaaa agaaatcagc cggacgaatt tatgagaaga ggattaggtc ttgcacttga
     7561 tccaattaca gaacttctga ttcgtcaatt cgataaatta aataaatagg aggaacaaca
     7621 atggctaaaa caacaacagt aacaactttt gacaatataa gtattaaaag aatttctttt
     7681 aattttaaga atgcaacaaa tgcaatttca actgattgta atggacaatt agatggcgaa
     7741 acagaaatgc agagaattgt aaaaaaatgc ggttcaacag aagtaaaatc gaaatctaaa
     7801 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgcttc
     7861 aatgggttga aacaagatga acgtattaaa tcgggaattt actcttatgg tcctgattcc
     7921 gtaggggaag atttctctct tgctgcagag atcgtggatg atttcgaaga aaaaaataag
     7981 ctgattggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
     8041 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
     8101 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
     8161 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
     8221 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
     8281 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
     8341 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
     8401 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
     8461 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
     8521 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
     8581 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
     8641 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
     8701 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
     8761 aactatgcaa taaaccaatc ccagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
     8821 gagaacagaa gcagaagtaa cattcatagc taacgatgac ggattgaaat ctacgttaaa
     8881 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
     8941 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
     9001 gtatgaatta caaagtcaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
     9061 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
     9121 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatgcac tagctcaagc
     9181 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
     9241 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag caactgctgg
     9301 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
     9361 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
     9421 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
     9481 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
     9541 cggaagcaaa atagatatga ataatctaat ggaagcttct gacgttttaa gcgacattgg
     9601 cgataagctt acagaattag ggaaacaagc agtggactct gctaatagtg taggtagttc
     9661 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
     9721 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
     9781 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatatcac
     9841 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
     9901 tgtaaatggc ttaatggtca atttcggctt gagcgctcaa gatgcaatgg atttaatggt
     9961 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    10021 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    10081 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    10141 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    10201 aaaagctttt gaagagtgga aaaatggcgg aggaacacaa aaagacgtta ttaattccat
    10261 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    10321 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    10381 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    10441 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    10501 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    10561 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    10621 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    10681 aataatagga gcagttatta ctgttattgc tggagttata gcaattataa aaaactgggg
    10741 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    10801 ttgggaaagt ataaaagaag gtgcctcatc agtttgggat ggagttaaag aaacctggtc
    10861 tggatttgta gattgggttc aagatatttg gcaaggagtt tctgattggt ttggagagct
    10921 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    10981 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    11041 ttgggcagac attgtaggaa ttgttcaaat accatggacc ttaataacgt cattgattca
    11101 agccggtatt aatattatcg tgggtatttt tgatgtagct ggacagttat taggcgcagc
    11161 ttggcaagct gtttggacac ctatttctga tttccttaaa aacacttggg atactatgac
    11221 acaatgggta agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    11281 agtagtggca tggtggaatg gtatatggac agctattagt actacggctt caaatatttg
    11341 gaattcaatt agtgcaacag cttctagtat ttggaacagt atcaagaata caatcactag
    11401 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    11461 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggaata gcgtgactag
    11521 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    11581 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    11641 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    11701 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gtacaattgc
    11761 taacatttgg gaaggtatca aaaagactgt tagcgctcca attgattgga tcagagataa
    11821 aatcagtagt atctttgata atttgaatat ttctatacca catattccgt taccacattt
    11881 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacgttgg gtgttgattg
    11941 gtatgcgaaa ggtagtgtgt ttaattctcc gaatattatc ggtgtcggcg aagcaggacc
    12001 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    12061 tagcacatca gtttcatcta gggcacaaac gattcaacct gtgaataact acgaattcaa
    12121 tttcacaatt gatggtaacg cagatgaggt tactatgaag caaacaactc aacaaatcat
    12181 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    12241 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    12301 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    12361 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagta
    12421 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    12481 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaattatgca
    12541 aagaaattag aattctctga tgatcccaac gtctattatg ttatccagac tatcgatatt
    12601 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    12661 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    12721 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    12781 tcatgccagt tcatcttaaa tgattatgtg tttagttttg aaggcttagt agtaggaact
    12841 gacgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    12901 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatattaat
    12961 tattttaatt gtactggttt gagcaaaata caaatttatc gtaatgggtt aaggtaggtg
    13021 aaatagatga tcgataattt aataactatt tacgataaaa acgacgcgaa taatttagct
    13081 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    13141 agcaataaat taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    13201 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    13261 cagcgtctac ggatttatta tgcaaagact tctgtaatag gcaatacgat agaagtaaaa
    13321 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagtggaaca
    13381 gaaaagatca ctgctagtca ggcatggcaa aacgcaaaaa ctttagcgaa accagttatt
    13441 ccttcacagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaag
    13501 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    13561 gaatttctaa aagataataa cacattacgt catgaaaaaa ggctaggcac ggatcataaa
    13621 atcaaagcga tttatactaa aaacttaact ggtcttgact tagagattga tgctcaaagt
    13681 gttttagttg gagtttatcc attcattagc agctcttcag aaggagaaga cgagatcact
    13741 ctaccagaag aagttatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    13801 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    13861 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    13921 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    13981 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    14041 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    14101 aacttcttag aaaacacaga gaataatgtc agcaatttga ttaatgatgc cattgatcaa
    14161 ttgaaaaacg gtggcgaaat cagtgattta ctcaatgata ttgtagatca tcaaactgat
    14221 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    14281 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    14341 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaacggc atggacgtta
    14401 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    14461 ggtactacat taattagtga tggtgctgat tatagaacaa gtattgctaa tggcaaaatg
    14521 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    14581 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    14641 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    14701 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    14761 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    14821 actatgaaat ttactagtga tggtatgcta acgttaccag gttgtttttt tggttcatgg
    14881 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    14941 gttagaggta ctaaaaactc aactgtacca acagaacatt atggacaacg actattgaac
    15001 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgacaat
    15061 ggtaaagttc gtgttgatat tgaccccatg tttgctgaaa cagtaaatct aagtcggtat
    15121 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    15181 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    15241 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    15301 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    15361 atttggcttt agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    15421 gtgataaagg gattacggtg acggttaaac cgtttctaaa tggcttgcag atggatacga
    15481 gtggcggaac atttacttta aaaggaacaa caccatctaa ccgttacgta gataatgttg
    15541 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    15601 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    15661 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    15721 atgtttcgca attagaagag ttgattcgaa agtacaacga aacttttgat gcttttatgg
    15781 ctgaaatcaa aggtagagtg gatagcttaa atcaacagat tactgattta actggtcaag
    15841 ctaaaacgct acaagacaag ttagatgctc tgaaagaaga aatttctaag ttaggtaact
    15901 tacaagtgat gtacagtaac agcatcgact tcgggaatta tgattatagt gggaatccga
    15961 atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg tcactaactt
    16021 cggataacga aaagctacat tttacttctg atggtacagg aagcattatt atgtttacgc
    16081 gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg aaagttcgat
    16141 ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt acatcaccag
    16201 gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat gtagggaaag
    16261 aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta gatcgatttt
    16321 atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag ttgtacgaca
    16381 tcaaaatcga agaaggctca acagccaccc catatcagcc aaacttacta gatggccctt
    16441 actggttagg taaagttcct ttaggcgaga atattgccga tcatacagtt aagttcccaa
    16501 tcgaatctgg cgaccaccaa atatatcaag gttacacaga agaagagctt atgataggtc
    16561 aaacgtatac tatcacgctt aaaggaacaa aacccgcaag tcaaaccttt gtagcgtata
    16621 atcattggac tgctcgttta ggagaactaa agccagttga tgggttgaca gacgtatggt
    16681 ctctaacatt cacaccaacg aatgttgtgg cgagttcacc taaacttttt cgtgtttatc
    16741 agtatccaca atcaacagta ggcgcatgcc ggatcgactg gctcaagatt gaaaaaggcg
    16801 acacacgaac cccgaatatt agtgagtata aatatcgtgg tactggtatg cgtgattcaa
    16861 acaatccaaa agattatgtt tgggatctag caccagaata tgtcgaagat aacttggcca
    16921 cagatattaa aatttctgaa attactggta aagcaaacaa ttataccgat gggaaagtat
    16981 cggagattaa ttcgcagttg actgcttcaa ttaatgaagt agacaccaca gctaaggatg
    17041 ctcaaacaaa agcgaatgct aatgcgactg ctatagatga attagacaat aagatcgatg
    17101 aacgcattaa tgatacagct actaccacat taacagttac aaacgggaat accggatcag
    17161 caaagcttta tcgtgaagga aaaacagttt ctatatattt tgtggcttta aacggaaaaa
    17221 gcagtggtgg aaatgattca acgatactaa caattccaga aggctatcgg ccaccaatta
    17281 gttttgagca actggttggc tcgatagacc gttctacttt gaacagtgct cagttatcta
    17341 ttggtgcaga tggagccatt aaatggcgaa gaaactcaag ttatggatcg gattatacct
    17401 ttgcaattac ttacacgatt tagaaaagcg tgaatcgata tgaaggcagc atatagacca
    17461 attgaacctt acggattcga gcaaatcatt gtgaatgatg aagaacattt accggaagaa
    17521 tgcacagaag tcgaaccacc gattccaaat tggaaaccga aattcaatta ctgggaggga
    17581 aataaatgaa aaacatttgg aaatatggac gtactggcgg agagtacgca ggaaaagtat
    17641 tggacgacat gcttgtatcc gttccttaca cagatcagcc accgcttgaa ggaattcgtg
    17701 ctgatggcga accgttaaca atcgctgatc aaatgtttga tcctaaattg aatcaatgga
    17761 ttattttagc gaacgcacta gatcacaacg atttaaacaa tctcaaagca atgtatgagt
    17821 cgttagaaaa tgagaacggc gatttaaaac agatcaatgc caaactcatg ctaagcgatg
    17881 tagcaattaa acaggaaaat actgcattga aagaaaaagc ggatagttta gcacaaatca
    17941 attcaaaaat gatgcttgct tcgttacaaa atagcaaaga catttcagaa attaaagagc
    18001 aactaaatcc agcttcaaag ggaggtgagt agtatgttta gttttagcga tgtgaaaatg
    18061 atgtatgatt ggggctgttt cactaacgaa caagtaatgg ttttcgttcc gttgtgcatt
    18121 actgaagaa
//
LOCUS       DBNICI010000045        17689 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.045, whole genome shotgun sequence.
ACCESSION   DBNICI010000045 DBNICI010000000
VERSION     DBNICI010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.045"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(59..187)
                     /locus_tag="AC1ZCC_001258"
     CDS             complement(59..187)
                     /locus_tag="AC1ZCC_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIQ1629522.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="AC1ZCC_001259"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="AC1ZCC_001259"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIQ1629523.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="AC1ZCC_001260"
     CDS             complement(1674..2729)
                     /locus_tag="AC1ZCC_001260"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HIQ1629524.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="AC1ZCC_001261"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="AC1ZCC_001261"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIQ1629525.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="AC1ZCC_001262"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="AC1ZCC_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIQ1629526.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="AC1ZCC_001263"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="AC1ZCC_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIQ1629527.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="AC1ZCC_001264"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="AC1ZCC_001264"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIQ1629528.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="AC1ZCC_001265"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="AC1ZCC_001265"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HIQ1629529.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="AC1ZCC_001266"
     CDS             10491..12050
                     /locus_tag="AC1ZCC_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HIQ1629530.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="AC1ZCC_001267"
     CDS             complement(12161..14092)
                     /locus_tag="AC1ZCC_001267"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HIQ1629531.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="AC1ZCC_001268"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="AC1ZCC_001268"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HIQ1629532.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="AC1ZCC_001269"
     CDS             complement(15021..15773)
                     /locus_tag="AC1ZCC_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIQ1629533.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="AC1ZCC_001270"
     CDS             complement(15932..16141)
                     /locus_tag="AC1ZCC_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629534.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="AC1ZCC_001271"
     CDS             complement(16152..17480)
                     /locus_tag="AC1ZCC_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629535.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     NQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..>17689
                     /locus_tag="AC1ZCC_001272"
     CDS             17616..>17689
                     /locus_tag="AC1ZCC_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HIQ1629536.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKT"
BASE COUNT         4664 a         3811 c         3200 g         6014 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gattttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat ttaattataa
    17521 ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaac
//
LOCUS       DBNICI010000046        17656 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.046, whole genome shotgun sequence.
ACCESSION   DBNICI010000046 DBNICI010000000
VERSION     DBNICI010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.046"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..284
                     /locus_tag="AC1ZCC_001273"
     CDS             <1..284
                     /locus_tag="AC1ZCC_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1629537.1"
                     /translation="YEKEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISK
                     AKLFLTETRLTLTEICEKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            281..2017
                     /locus_tag="AC1ZCC_001274"
     CDS             281..2017
                     /locus_tag="AC1ZCC_001274"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing sensor histidine kinase"
                     /protein_id="HIQ1629538.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(2080..2916)
                     /gene="pheA"
                     /locus_tag="AC1ZCC_001275"
     CDS             complement(2080..2916)
                     /gene="pheA"
                     /locus_tag="AC1ZCC_001275"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIQ1629539.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(2913..3422)
                     /locus_tag="AC1ZCC_001276"
     CDS             complement(2913..3422)
                     /locus_tag="AC1ZCC_001276"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIQ1629540.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(3415..4710)
                     /gene="aroA"
                     /locus_tag="AC1ZCC_001277"
     CDS             complement(3415..4710)
                     /gene="aroA"
                     /locus_tag="AC1ZCC_001277"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIQ1629541.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPISVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(4723..5814)
                     /locus_tag="AC1ZCC_001278"
     CDS             complement(4723..5814)
                     /locus_tag="AC1ZCC_001278"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HIQ1629542.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(5831..6997)
                     /gene="aroC"
                     /locus_tag="AC1ZCC_001279"
     CDS             complement(5831..6997)
                     /gene="aroC"
                     /locus_tag="AC1ZCC_001279"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIQ1629543.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(6999..8063)
                     /gene="aroB"
                     /locus_tag="AC1ZCC_001280"
     CDS             complement(6999..8063)
                     /gene="aroB"
                     /locus_tag="AC1ZCC_001280"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIQ1629544.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(8096..9118)
                     /gene="aroF"
                     /locus_tag="AC1ZCC_001281"
     CDS             complement(8096..9118)
                     /gene="aroF"
                     /locus_tag="AC1ZCC_001281"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIQ1629545.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(9777..10751)
                     /locus_tag="AC1ZCC_001282"
     CDS             complement(9777..10751)
                     /locus_tag="AC1ZCC_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HIQ1629546.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(10732..12615)
                     /locus_tag="AC1ZCC_001283"
     CDS             complement(10732..12615)
                     /locus_tag="AC1ZCC_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HIQ1629547.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(12633..14858)
                     /locus_tag="AC1ZCC_001284"
     CDS             complement(12633..14858)
                     /locus_tag="AC1ZCC_001284"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004557 - alpha-galactosidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HIQ1629548.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            14973..16007
                     /locus_tag="AC1ZCC_001285"
     CDS             14973..16007
                     /locus_tag="AC1ZCC_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIQ1629549.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMMYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(16074..17087)
                     /locus_tag="AC1ZCC_001286"
     CDS             complement(16074..17087)
                     /locus_tag="AC1ZCC_001286"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIQ1629550.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(17165..17353)
                     /locus_tag="AC1ZCC_001287"
     CDS             complement(17165..17353)
                     /locus_tag="AC1ZCC_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HIQ1629551.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         4906 a         3836 c         3248 g         5666 t
ORIGIN      
        1 attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct
       61 atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag
      121 tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct
      181 gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag
      241 gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga
      301 gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat
      361 cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca
      421 atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat
      481 cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga
      541 tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc
      601 tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag
      661 aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc
      721 gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg
      781 attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac
      841 attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct
      901 ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac
      961 gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact
     1021 aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg
     1081 ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt
     1141 gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt
     1201 tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct
     1261 agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct
     1321 agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca
     1381 agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca
     1441 ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga
     1501 agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga
     1561 agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag
     1621 tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt
     1681 atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca
     1741 caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt
     1801 cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca
     1861 tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc
     1921 atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc
     1981 gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt
     2041 ttattttaag ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac
     2101 tgtcagcact ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg
     2161 gagcgcattc tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata
     2221 ttctcccaag ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca
     2281 gccaaaagct gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat
     2341 gacactcatt tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca
     2401 aaaacgtgtc tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac
     2461 atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac
     2521 atacattgct gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt
     2581 ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt
     2641 tgttgctgga ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc
     2701 gacttctgga tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt
     2761 ctctacagga acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg
     2821 gatggaagca tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt
     2881 gaatgaactt tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg
     2941 gtatcgatga tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct
     3001 tcataccaag aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga
     3061 gctaaaggtc gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct
     3121 gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg
     3181 acaatccctc caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct
     3241 gactccactt ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat
     3301 tggatatgtt cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct
     3361 aaccctttac cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc
     3421 atgagcttct cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat
     3481 tgaaacagct tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg
     3541 cagcatcata ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt
     3601 cggcccatgg atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa
     3661 ttcttctgcc accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt
     3721 gatgatcgtg attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat
     3781 cagacgcgga atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact
     3841 taaatcagcc gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc
     3901 ctccaacaca tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag
     3961 ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc
     4021 tggaacagtt atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc
     4081 ttccactgtg atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg
     4141 ttctttttca acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga
     4201 tttgacttgc gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc
     4261 tgtcacagag attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc
     4321 gcgtaaaggt tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag
     4381 agtagtcgta aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga
     4441 gtttcccatg tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat
     4501 cttgatcacc tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt
     4561 actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc
     4621 tccaaacatg atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct
     4681 taatccatgt atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg
     4741 ataactggta ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa
     4801 actgatttgc agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa
     4861 agaaactccc gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg
     4921 cacatcgata aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg
     4981 gtctcgactt tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat
     5041 gatccattct gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat
     5101 ctgcttattg ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt
     5161 gatatctcga aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg
     5221 ttctgcctga ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt
     5281 tatacggtca tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag
     5341 ttcttgcagt ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc
     5401 attttcaaat agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg
     5461 atgtccaccg acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt
     5521 actgctgaca tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag
     5581 ataagctaag gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc
     5641 tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt
     5701 ttttgactga ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag
     5761 tgccatagaa gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt
     5821 tgcccccttt ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact
     5881 caacaagcga atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa
     5941 cattttccgc aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg
     6001 ctttgtaagg ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa
     6061 tgggtttcat gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc
     6121 cgcctagatt attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca
     6181 tcacttctga gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa
     6241 aggcattgat actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta
     6301 cgtagcttcc taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg
     6361 tatctccatt tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat
     6421 caacaactcg tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc
     6481 caggtatttc tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa
     6541 gttcttccag aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg
     6601 cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca
     6661 aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca
     6721 cttctgctac agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag
     6781 tgacaggtga accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt
     6841 tttcgatctt catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat
     6901 tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt
     6961 ctggtccatg ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct
     7021 ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg
     7081 atattcaatt ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct
     7141 ggatgactta gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca
     7201 gctgtcgatc cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt
     7261 ccaatagcta ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca
     7321 tggccaattg tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct
     7381 tccactactt ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga
     7441 agatcttttt cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct
     7501 gctgatttga tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga
     7561 acctcaggat caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct
     7621 ttgtttgtat tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta
     7681 gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga
     7741 tcacccacca ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct
     7801 gctaaaaaat cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt
     7861 acgattgaaa ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca
     7921 tatagtggag caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt
     7981 tgaagccatg atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg
     8041 ttatcaacat gtacagtaag cattcgtttc acttctttct tttaaaaagc tggaattaat
     8101 cgagtgtctg aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg
     8161 agtaagtctt ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat
     8221 ggatctctac gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta
     8281 caagttccca aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt
     8341 ttttcatcaa tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc
     8401 gaatcccacg ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag
     8461 cattaagcca ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg
     8521 ttttaccgac agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga
     8581 tatcgctgta ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt
     8641 tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt
     8701 ggaaagcata tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc
     8761 cggctttggc cattcgcgca cattctctga tttggtctaa tccttcgata gaacaaggac
     8821 cagccatcgt cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat
     8881 tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt
     8941 ctactccttc atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc
     9001 caatcactac tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat
     9061 gatcgacgac tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg
     9121 tttttcccgc tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag
     9181 gtggttttac attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt
     9241 ctttcctact tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc
     9301 tacagtgaac atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat
     9361 agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac
     9421 aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa atagctaaag
     9481 taatacgtat tcagttttgt aagttttcta gatttcataa aaaatcgctc caaactgatg
     9541 taagaattgt acctagtata gacataaaaa aatcgactgt caatcatttt tatgaaactt
     9601 tttttaattt tctgatagtt aactttgttt ttcagaaaaa taacaattgg ttttattgaa
     9661 gaaaatagtc atgaacaaaa gtatctgaga acttttttac tctcttgttc atgacatata
     9721 gaataaagca gctttgtctt gtaaactccg tttttcaaat aataatctaa agattgctac
     9781 tgaataataa agccgatttc atggtcctct tcgctgctga ttcggtaagg tgactcaaca
     9841 tccgctcccc agctattgat acccccgact cctcttactt ttgcaaaaat cgtaagaacc
     9901 gttcgtcttt ctggcggtag ttcttctata tgtgttgcct gttccagttc ttctgctttg
     9961 aatggtaaag cggaaaaagc aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct
    10021 tcttgatctt tatccgtatt atccaacgtt tgatttcttg ttagttctac ccatttcgtt
    10081 tccatatgca tgccgcattc ttggggaaca aggtatgggg aaacggggag tcctgccact
    10141 ttataaatac cttcgacacc accagccatc cgatccggat atgtttcccc tgataggcct
    10201 tcatactcaa aaaattttgc tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat
    10261 tccggtaatc ctttttgtcc ataataatgc atattgacaa gcattttacc ctctccagcg
    10321 atttggtagg aaattgtcac ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg
    10381 atttcaaccg tttcagcgta ttcattactt gaatattgat tatttactgg agcaatcggc
    10441 aaatcgatct ttttattgtc caccagtact tctatttctt tgacttttgg atacaatccg
    10501 gctcctaacc acataccgga tttaaatcca aatccgctac ctctgtcatt atcggtcgtt
    10561 gctcgccaaa aaactggaag tgtttcccgg tatagccatt cttttccgtt tttattgagc
    10621 gattccagtc cgcctcttgt ataagaaaaa atataatgcg tattccccga tttgacacct
    10681 aatgtacagt cgccgaagac gattgcaact ttttccgtat ccatatgact tttatttact
    10741 gtatttttca taataatatc ttacctcctg catggcgggt ttttcttgtc catctgcaaa
    10801 aacgatccca ttccctgaga attcatagtc acttggttta tcgtcaaagt caccgccata
    10861 gcgcatcact tctctgcctg tgattggatc agtcacttgg atcgcctgat ctttaaaatc
    10921 ccatatatag cctccttggt acatttcata acgatcaagc aacgtcatgt aagaatccat
    10981 tccacctaac gaattcccca tgtcatgcat atattcacat aagatgaacg gtttttccgc
    11041 tttattttca aggtagtcaa tgatctcttc tggtggcgca tacatgcagc tttttacatc
    11101 tgaaatcgaa tcttcgaatg ctggatttgg aaaattccct tcataatgga caagtcgatc
    11161 ggggtctact tttttgaagt aatcgttcat ttgtcgaatg ttttcttccg cataagattc
    11221 atttccaaga gaccaaaaaa gaatacttgg atggtttttt aatgtttcga aattcgtttt
    11281 tgctcgatca agaacagccg cctgccactg aggtactgca cctggtacgt tgtaagaagg
    11341 ctcaaatgcg cccatttttt gccatgagcc atgggattcg aggttcgttt ccgccatcac
    11401 atggattcct gcttggtcgc ataaataata ccaagtgatc tggttggggt aatgagatgt
    11461 ccgtacgccg ttgatctttg cattttttat gatagccaaa tctttttcca tatcttctat
    11521 agtgatggcg cgtccttttt tcggatgcca ctcatgtcga tttaccccgt tgataatcat
    11581 ccgttttcca ttgaagtaga tgattttatc tttgatttcg attttacgaa aaccgaagga
    11641 atagtcaata tactccgtta ctcttttttc ttcgtctatc gtctccaatt caagcttata
    11701 taaattgggc tgaaaatgcc cccacagtgc cacattttca aatccatcga tgcgaatcgt
    11761 catttgctct tttaacggat attgctgttg atagatttcc ttcccatcag gatctagtat
    11821 cgctacattc agctgactgt ttttggctac ttgctcaaaa gacaaatcta cttcaagttt
    11881 gccattttga catttgtcat ctaagcgagg tttgatccaa agatcttcta aatggttttg
    11941 tcctcgagca actaagaaga catctctgaa gatcccaaaa aatcggaaaa agtcctgatc
    12001 ttccaagaat gcagctgtac ttcttttgta aacctccaca gctagaatat tttcttcttc
    12061 caaataaggt gttaaatcaa attcagacgg agtgaacgaa tcttcggcat acccgataaa
    12121 atgaccattc aaccaaataa acattgcttg ttctacacca gcaaaatgaa tcgagatttg
    12181 ttttcccttt agaccttctt ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc
    12241 tttagcagct gaaaagatcc cttccgcctc tttctcaggg cttgtcgtga aagggggacg
    12301 gcgaaataca tgtccctccc acggatacat cgtattgatg taatggatct tgtcataatt
    12361 tgctaattca atatgactag gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt
    12421 tttataaaat gtttttggtc gtaccgaagg atttggcgaa taacaaaaac gccatgatcc
    12481 atttaaagac tgtttaaggg aggatgtggt ttctacttct tcccttgttg cataacaaac
    12541 atgatcgcta tgtgcatttg attggttcac tctaaaaact tgtggatcat ctaaccattt
    12601 tatatttgct ttcatacttt tttccttctc tctcataact ttttgaaatg aagcagttct
    12661 gtttggaaat caccttgaaa atcggggaag taaagtccaa ctgtcatcaa ttcggccccc
    12721 gaaaatatcc gcttcgaggt ctgttcctga taaagtgcct catcatctaa ccccatcaga
    12781 tatacttcat ggaacgctgg ttgagctgaa gctagtatcc tccctaaaaa cacaatagct
    12841 tcatcttgtt taggagacac aaacatccat gcggcaatat tttcttcaaa aggactcgcc
    12901 aaacgataat aatggccata ctggatcacc ggacgaatcg tttgatagga tataatttgg
    12961 tcttttactt gctttttttc ttcaggagac agctttgtca aatccagttc ataaccaaaa
    13021 acagcgctca tagctgtttc tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga
    13081 tttggtacag ctgatacatg agctgtgatg gacgaaatcg gataagcaag gcttgttcca
    13141 tattggattt tcatacgctc cactgcatcc gtgttatcac ttgtccatga ctgcggcata
    13201 taataaataa acccggcatc aaaccgtcca cctccaccag aacagccttc ccataagatt
    13261 tccggataat cagttgtcaa atgttcaagt agttcataaa gcccgagcat ataacgatgc
    13321 gctacttctc cttgtctctg tgggtcaagg gtaatcgaat agacatctga aagcgaacgg
    13381 ttcatatccc atttgatgta gtcaagagga atcgtatcta aaatctttct catttgtttc
    13441 tcaatcgtct gccgaacatc taaccttgta aaatccagta catgttgtga tctggaagca
    13501 gaaggcattc tacctggctc ctgcataaga aaatcaggat gtgttcgata cagttcagaa
    13561 tcaaccgaga tcatttcagg ttcaaaccat aaaccaaatt tcaatccttt gctatgagca
    13621 taatctgcta tttcacgtaa tccatttgtc aacttgcctt catactcgaa ccagtctcct
    13681 aatgaagaat tgtccgcatc tctttttcca aaccagccat catctaatac gaacatttcg
    13741 atgcctagct cctttgcttc atcaatgatc gccttgattt tttcactatt gaagtcaaag
    13801 taagtggctt cccagttatt gaccagaatc ggccgttcag caaattgatg acgacctctt
    13861 gcgattcttt cacgtagcaa ctcatggtgg atctgactca ttttgttcaa tccctgactt
    13921 gagtaagaaa ggatcatttc aggtgtttga aaagactggc ctgcattcaa ttcccacgaa
    13981 aaacgatggc tgttgatccc accgacgact ctcaattgat tgatctgatc tttttcaagt
    14041 tcgaaagaat ggttgccgga ataaacaaat tgaagtccga tcgcttctcc agtattttct
    14101 gtcgtatgat gatgaactaa agcaataaag ttattcatat ggtgactgct cgttcctcga
    14161 cggctttcaa atacttttgt tccagataaa acagattgcc gtgaaatctg tctttcattg
    14221 acatgtgctc ctggcaaagc aatgacttca tcaaatcgcc tagtatgggc aaagtctagc
    14281 tggaaactag ccgctttttc tagtttgatt gtttctccgg tttcatttct tatctttaca
    14341 gatcgtgtga tcactgctct atcagtaaat atggtataga acaaatcgaa ataaatcgct
    14401 gtttctctat ccttaagtga aatcaccaac gtttgtgcct ctgacttgtc catcacataa
    14461 gtttgaggca agccttcgat ctctggcttc ccatctgtaa tataatggct gtcatatctc
    14521 agatccagta agtctgatcc attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc
    14581 atgctgccat gagaagagta ttcttgcggt agtgtatctt tcgacagaga ccggtcagga
    14641 tttaagggaa catttcctga aaatccacga tctcttcggg gataattttt attgtctttg
    14701 tactgcttga ctggtttgcc aaaataaata tgcgccaata cattgctttc ttctatcttt
    14761 atcagataag aaagaaatgt atttcgtaaa tggaagactc ggctcttttc atcaaataag
    14821 atgaaatttt tatctgtttt tctatgcgta atatccatca tttcactccc aacaaaacgg
    14881 aaaccgttta ctttttttta taattcctgt atactgttta ctaaacaatt ttgtcaataa
    14941 aaatttttta tttttttgag gagtgtgtaa aaatggttgg aattagagat gtcgcacgaa
    15001 aggcaaatgt ctctcctgct acagtttccc gtgtgttgaa taacgatcag acgatccatg
    15061 tggctcagac tactcggcaa cggatagtag aggcagctgc tgcgttggat tatgacatca
    15121 gtactcgtaa atatatcaaa aagagaatgc cttcgattgg tgtgatctct acgatcggca
    15181 aagcgagcga agaacaggat atctattaca aacatttgcg agtagggatg gaagaagaag
    15241 ctcgacgtct gcatttaggg atgaatcgaa tctataatct atctgataat cctaaacaat
    15301 gggaagatct agatcagtta ggtgcggtga tcgttgttgg gactgttacg aaagaatcga
    15361 ttaccgcctt gttaaaacaa aacatgcaaa tagtcgtcgt ggataaccct gacatccagc
    15421 aagaggtcga tatgatgtat gcagatcttg aacggatgac taaaaccgta ctggaattgt
    15481 tcttagaaaa tggtcataaa agaatagcct atatcggggg atataaagtc gatatggatg
    15541 aattcggtgg gaagcagatt tctataaatg aaaagaggat gcgtgcctat cagcatttta
    15601 tgatcgatca tcacttagaa aaatatatgt cttatcatct aggtggctgg acagaaaaac
    15661 aaggtgaggt acttgcgact aatctattag aaaatgaatc ccctcttcct actgccattc
    15721 ttgtcgggag cgatccaatg gcaattggtg tctatcgggc attgaaacaa aaaaatatcc
    15781 aaattggaga agagattgtt gtagcaagct ttgataatat cgagacaatc gcaaacctct
    15841 cccctggctt gaccagtgtt caaatcaatg cgaaatcaat tgggcaagca gctgttcgaa
    15901 tggcattgga gcgaattgac ggtatacggg acgaaccaat cattttgact tatcctgcac
    15961 gattgattgt tagggaaagt tttatcccaa gaacaaaaag gtattgaaag agaggttgtg
    16021 aaaagccgct tcgtttcttc atactcaaaa cgaaacggct ttttcacaac ctcttactta
    16081 tacttattca caaaatccaa tgtctgctgg taacaatttt gcgattcttt tagcgtgtaa
    16141 tcaaattggt attcatgggt aatctctttt tttgtatctg tataaaacaa ggattgcacg
    16201 ggtaccttta attctgtcaa ccgattttct aatgcttgac cttgctcctg gaatgagtat
    16261 gcatttccgt cagttatata cgttggcgga aattccttag aaacatggtc agccacacta
    16321 gcttctttca attgctcact cgttttccaa tcttttgttc caatcaacga ccacgcaacg
    16381 gttttgacga aaaacttcat aaatcgatca tccgaatgct gctgtgctgt tagcttcaaa
    16441 tcaacaggtc cgcaataaga aatggttcct ttcagactat cttttggaag tagctgtttg
    16501 attttcattt gtttagcata cgcatcattc gtttgtatag ctgcatattg caatgcgatt
    16561 tgtgaacctg cactatctcc accaaaaaat aattgttcca tgtctagcaa tttgtcgtct
    16621 ttttcttcag ctaataaatc tttgatcagt tcattcactt gaagaacttg attagggtat
    16681 tctgaatcag gcgctaattc ataattcatc gcaaccactg cgatgttggc gtctgaaact
    16741 aattttgtcg caaattcttt gacacccgat ttatctccgc cgacgaatcc tccaccatgt
    16801 acccaaaata gaacaggaac aggccccttg ctgttttttg gatagtagat gtcataagta
    16861 ttctctttat acttggactc atacgtcaaa tctgtttttt tcgccacgtt tttttctgct
    16921 tgatcgaacg tttttttatc agtgatttga acagtatttg aaaacatatg gttgatcaag
    16981 aatgctccag gtctggggga aagctggaag gcaagaaaga ttgcaaacgc gatcccaaga
    17041 atgattccga agaaccatcc gataaattta agaattttct ttttcataag gctccctcag
    17101 tagttagtat taagcattct gattatacca tagtgttatt tcctatcaag ctctcctaga
    17161 tgatttaatg attttcccca aacttcttct gcaatgcttt tgacaaaaga aattttttcc
    17221 attgttcttc aaacaaaaca ttttcttctt ctgttgaagc aaatccacat tgaggactta
    17281 agcacaaacg atccaaaggc agatatcgtg tcgcttcgta gatccggtca atcacttgct
    17341 ggtgctcttc catttcgcca tttttcgtta cagtcaaaat ctaagacaac taatttatct
    17401 tcagagactt tctctaaaag aacaaaactc ccagcacgtt cactatcgta ttctaaataa
    17461 taggcacgga cattttcttg agtaaaataa tgggtctgct acgtcgtcat cccccctgat
    17521 gccgccaaaa tcgaataata atttccgcgt caaacatgta tatttagtgt tgaatcattt
    17581 gagaatatga aaataaaaat ctttttgatc aatcagttaa tcaaattgaa ataacgaaaa
    17641 atctgagaca gaatga
//
LOCUS       DBNICI010000047        17317 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.047, whole genome shotgun sequence.
ACCESSION   DBNICI010000047 DBNICI010000000
VERSION     DBNICI010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.047"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(567..1592)
                     /locus_tag="AC1ZCC_001288"
     CDS             complement(567..1592)
                     /locus_tag="AC1ZCC_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIQ1629552.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1867..3552
                     /locus_tag="AC1ZCC_001289"
     CDS             1867..3552
                     /locus_tag="AC1ZCC_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIQ1629553.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3566..4849
                     /locus_tag="AC1ZCC_001290"
     CDS             3566..4849
                     /locus_tag="AC1ZCC_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIQ1629554.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4919..5788
                     /locus_tag="AC1ZCC_001291"
     CDS             4919..5788
                     /locus_tag="AC1ZCC_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1629555.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5785..6621
                     /locus_tag="AC1ZCC_001292"
     CDS             5785..6621
                     /locus_tag="AC1ZCC_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1629556.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6647..7714
                     /locus_tag="AC1ZCC_001293"
     CDS             6647..7714
                     /locus_tag="AC1ZCC_001293"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIQ1629557.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7711..8538
                     /locus_tag="AC1ZCC_001294"
     CDS             7711..8538
                     /locus_tag="AC1ZCC_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIQ1629558.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8583..9608
                     /locus_tag="AC1ZCC_001295"
     CDS             8583..9608
                     /locus_tag="AC1ZCC_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIQ1629559.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9605..11788
                     /locus_tag="AC1ZCC_001296"
     CDS             9605..11788
                     /locus_tag="AC1ZCC_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HIQ1629560.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11788..12918
                     /locus_tag="AC1ZCC_001297"
     CDS             11788..12918
                     /locus_tag="AC1ZCC_001297"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HIQ1629561.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12915..13592
                     /gene="pgmB"
                     /locus_tag="AC1ZCC_001298"
     CDS             12915..13592
                     /gene="pgmB"
                     /locus_tag="AC1ZCC_001298"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HIQ1629562.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13676..14203
                     /gene="msrA"
                     /locus_tag="AC1ZCC_001299"
     CDS             13676..14203
                     /gene="msrA"
                     /locus_tag="AC1ZCC_001299"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIQ1629563.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14404..15090)
                     /locus_tag="AC1ZCC_001300"
     CDS             complement(14404..15090)
                     /locus_tag="AC1ZCC_001300"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIQ1629564.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            15170..16708
                     /locus_tag="AC1ZCC_001301"
     CDS             15170..16708
                     /locus_tag="AC1ZCC_001301"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - obsolete D-gluconate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HIQ1629565.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16828..17295
                     /locus_tag="AC1ZCC_001302"
     CDS             16828..17295
                     /locus_tag="AC1ZCC_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HIQ1629566.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
BASE COUNT         5613 a         3136 c         3741 g         4827 t
ORIGIN      
        1 tattgtcaca acctcttttt tggtttgtct aaaataattt gtctaactag aagaaaagtg
       61 ggaaaggatc ttaacaacat cggttcgtat ccaattaagg tatcggaacg atcatcttcc
      121 aagagaagtc aatcaacaaa agtaaagata gatcattttt tgaatgcaaa ataaattgat
      181 gggaagatag agcggttatt tttaatattt tgctagctat tattgaataa ttcctcgaat
      241 aaaacaaaac cagtagacat gtctgttatt ctatttatgt tttagagata agtatcccca
      301 aataacatgg attgaaataa gggtaaatgt gtttaaaaaa acgaactata aaaacttata
      361 cacaacgtgg ttaaaaatag ttttttcttc cctgtggaaa aaatagttta acacaaacat
      421 tatagttatc cacagctatc taaaacagat ggaaaataag aaaaattcgc taaaataccg
      481 ggagggggta acgagggatg agtgattgaa aaaagaacag agtaactgac ttatcctcag
      541 tgtttctgtt cttttataat tctgttctac atttcttgtt gttggatcga atctgtgaga
      601 gtaatctcgg ttgaaagaat gattcttttt tcttcagggt attggtggtg gattagttgg
      661 aataaaagtt ctgtggcttt gattcctaac tgatttgtcg gaatgtctat ccgagtcaac
      721 tgtggcgaaa atagggagga caataaggga tcactgaatc ctgcgatcga aagatcatca
      781 ggtatctgct ttccagattg ttctgccact ttgtaaatac tcaatgattt taatgcatcg
      841 gtagaaaaaa tcgcagttgg tctttctcgt agcttcatta aatcattcat tgctgccatg
      901 ctatcttcta agcgaaagcc gccgtctata atccattcat tacggatatc taggtggtga
      961 tcaaataagc attgacggta accattgaga cgatcattgg atacataaac ttctgtcgga
     1021 gcatggagac agccgatatg acggtgtcca ttcttgatca gtgtttccac caaatcgtaa
     1081 ctatcctgga aattatccgt gtccactgag tagatgttcg tgtagtcgtt tccgactttt
     1141 ccgattacca caataggaat attgtattga tccaattcaa aaagaagtgt ttcatccatc
     1201 gtagagctta aaataataat tcctcggatc attttttctt ggatcttttt cgcgcatttt
     1261 tctagctctt cttctggtgt ttttgccgtt tgtagaatca agtcgaagtt attttgttca
     1321 gaaacggcag aaatggattg aataacatct gaaaaaaaag gattgccata ggtcgtatga
     1381 gaagtacgat gagataatgc tagaatcgcg tcaaaaccat ttcctgacag tcctcttgca
     1441 attttgctcg gttcataatt caattctttg attgcttgaa gtacacttgc ttttgcttta
     1501 tcagagatat ttggctggtt atttaatact ctggagacag ttgattttga gacattagct
     1561 tttttcgcaa tgtcataaat agtaggtttc aattttatat gcacctcctt tttgggagcg
     1621 ttttagaata attccatagt acaatagaca ttcttttttg tctatgattt tgaaaattta
     1681 ggaatagctt gcatgatcat gagagaccat atttttttaa gaagctatta attattgata
     1741 cataagcttt cttatttgta tttttgtgta tatcaaaatt atttaaaaaa atgctattga
     1801 cagtaagcgt tttccataat atgatcaaat cgttgggaac gctcccaaaa aagaaagggg
     1861 aatccgatgt atacaaaact tttacaagcg atttatcgcg actcagattt gtctgagaaa
     1921 accgcctcta tgattgaaca aaaagtcagt gtttatcagc agaagaaagg ccaggcaggc
     1981 aaactgcatt gggacgaaga agatattttc ttgattactt atggggatca attttttgaa
     2041 gagcagcaac caactttgcg tacatttaag aaattttatc aaaagtattt ggcagataca
     2101 tttagagttg tacatttctt gccatttttc ccatatacat ctgatgatgg tttctccgtt
     2161 gtaaattatc aggcaatcaa cgaccgtttg ggcacatggg aagatataga agaaatgaga
     2221 agcgaaacac ggttaatgtt cgactttgtc tgcaatcata tgtctgccga aagtacttgg
     2281 tttcaggaat atttaaactt gaatgatacg tacaaagatt tttttattga tttgccactg
     2341 gatacagata caagcagcgt gactagacca aggaccagcc cgttacttac aacgttcatt
     2401 gacaaaaacg ggaaagaaaa acacgtatgg acgactttta gcgaagatca agtagacttg
     2461 aattttaaaa atccagaagt tttattgaaa atgatcgata ttcttttgtt ttacttagaa
     2521 aaaggcgctt catttattcg cttggacgct gtgggatttt tatggaaaga actaggaact
     2581 tcttgtatac acctgccaga aacgcataaa atcatccaat tattccgtgc aatcgctgat
     2641 gaagcagctc caggaacggt attgatcact gagacaaatg tgccacacaa agataatatc
     2701 agttattttg ggaatggcga gaatgaagca cagatggtct atcagttttc gctaccacct
     2761 ttgacccttc aagcgattcg ttcgggtaat acaagtaagt tgaaagaatg gttaaaaacc
     2821 atcgatttcg catcgaaaaa aacaactttt tttaattttt tagcctccca tgacggtatc
     2881 ggattgaatc cggtacgagg tattctgccg gaaaaaacga tccttgaatt agtggaacag
     2941 ctgaagaaag agggtgcccg agtaaactac aagaaaaacc cagatggaac gaccagtcca
     3001 tatgagataa acgctacgta tctagatgcg ttaagttctc caacagatac gacggaatgg
     3061 agaagcaaac gctttttagc tgctcattca tttttggtaa cgttaccagg cgtgccggca
     3121 atttatattc aaagtgtgct gggaagtcga aatgattaca aaggcgtaga aaaaacagga
     3181 gagaatcgaa aaatcaatcg gcaaaaattt ttgttagcag atattgagaa agagttgcag
     3241 acagatccat taagaacagc agtatatggt gaattgaccc agatgctatc tattcgaaaa
     3301 aaagaaaaag ccttccatcc gaatagtttg atttacttac tggactcagc agattctttt
     3361 atcagtttta ttcgcgaagc gaaggaagag aaagtcttag ttatccataa tttttccaat
     3421 caatcagcag gttatctttt accaaaagga gattacctag acctgttttc tggacaaatt
     3481 atccattgca gtcaagaacg aaagttgata ttccagccgt atgaatatat gtggctgaaa
     3541 ataataaaat aagaggtgga aaaaaatgaa aaaaattatt gttactacct gtctgctagc
     3601 tactggattt cttcttactg cttgtggtga aagtcaaagc tctaacgaat cacaacagac
     3661 aacgattcaa ttcatgcact cttctgtcga acaggaacga ttaagtgtga tcaatgaact
     3721 ggttgctgat tttgaaaaag aaaatccaga catcaaaatc aagcaagtgc cggtagaaga
     3781 atcagcgttc aatacaaaaa tcgttacgtt agctcgttcc ggtaaattac cagaagtaat
     3841 ggaagtcagt caggattttg ctaaagtgat ggataaagat gaactgattg atcagacggc
     3901 agtccaaaac gttatcgagg aagctggaga agataattat tatgacggag ccaaaaacct
     3961 tgttcgttca gaagatggaa aaagctatat tgctgcacca attagcggct gggttcaagg
     4021 tatttggtat gacaaagaaa aattatcaga agctggtttt tcagaaccag aaaattggga
     4081 tgatatttta aaaattgctc aacatttcac agataaagag aataaacaat atggaattgc
     4141 tatgcctact gctgaaggaa caatgagtga gcaagcgttc tctcaatttg ctttgtcaaa
     4201 tggagcaaat gttttggatg atgaaggcga ggtaacgatc gatacggaaa agatgagaga
     4261 agcattgtcg ttttatcaaa acttatccca atacacgatg ccaggctcta atgatgtgac
     4321 agaaatcaaa gatgctttca tgaatggaac agcaccaatg gcaatctatt caacttacat
     4381 tttgccttct gtttatgaag cggggaactc agaaaatatc ggttttgcta tcccaacaca
     4441 aaaagatcaa gctgtctatg gcacagtatc tggattgacg atttcttctg gtctagacga
     4501 gaaacaaaaa gaggctgctg aagcattcac agctttctta tctcaaccga aaaacatgga
     4561 aaagtgggtc ttgatgtctc ctggtggtgc tcaaccagtg aataaacaag tagtcgaact
     4621 ggatggttac aaagaaaatg aagtcatcaa gtcttttggc gagctgccat ctgagattgc
     4681 tagtgctttt gatgaagtac aagtctttgg actagtcggg gaaaagaatt tcacgaaaat
     4741 gggagatatc acaagctcag gtgtgatcgg aaaagcagtg aaccaagtaa ctgtcggaaa
     4801 cgaagatgtc gatcaagctt tagctgacgc acaaaaaaat acgaaataac aagaagttct
     4861 gccaatggtg ttcgaaaaaa gtagatagct attggcagat tatcaggggg aggtttcaat
     4921 gaatcgtcca aggaaacatt ctgacttaaa attagccatg ggacttttag caccaagcat
     4981 gcttttatta gctgttttag tgctgattcc aatgctttcc aatattcaga tcagtttttt
     5041 cgatatgccg ctgaatccta actcagacgc cgtttttatc ggattaagca attatcttga
     5101 aattttaagt gatagtgaat tttatcgttc tttaggttta acagttgctt acacattctt
     5161 agttgtagca ggcagtacag ctttaggatt agccgtagct attttgctta accgcaagtt
     5221 cagatttagg aaaacggtgc gttcactgat tttattaagt tatgttacgc catctatttc
     5281 tttgattttt gcttggaaat atatgttcaa taatagttat ggaatcgtca atgatctgtt
     5341 agtagatagg ttgcagcttt tcaacgaagc accgctgtgg ttcgaccagc cagttgctag
     5401 cttctttatg gtcgttttgt ttgctatctg gagatactat ccgtatgcgt ttatttcatt
     5461 tttggctatt ttgcaaacag tcgacaaaac gctttatgaa gcagctgaaa tggacggtgc
     5521 aaatgcttgg cagaagttca agatcgtgac actgccggca atcaaaccag tactagcaac
     5581 tgtgattgtt ttacgatcta tctggatgtt ctatatgttt acagatgtct atctactgac
     5641 gaataaagtc aatatcttag gcgtttattt gtatcagaca gcctttgcgt tcaatgatct
     5701 tggaaaagca gcagctatat cgattctatt gttcatcatt attgcagcgg tcgtcttgct
     5761 ctcacgaaag aaggtggact tgaaatgaaa aataaaaaga gcaggaaaat cttattttac
     5821 gcgacgattt tgttgctatt agctgtcaat ttgtttccat ttttgatcat gctgatgaca
     5881 tcattcaagg attcacaaga agcaattgca acgacacaaa ctatttttcc gaaagaatgg
     5941 acttttgaac attatcagga tattttcaat gctgagattt ttccattcat catgtatttc
     6001 aaaaatagtt tagtgatctc cttgatagcc tccgtaactg cagtagtgat tgggatcagc
     6061 ggggcatacg ctttgtcaaa actacgcttc aaagctcgct cgaaaatcaa tgctagcttt
     6121 tacaccgttt atatgttttc cggtattttg atcgttgttc cattatttaa aatcatttcc
     6181 ggtctaggtt tgtatgatac caatacagca ctgattatca cgatggtcgt tcaaacactt
     6241 cctactgcga tattcatgct aaaaagttat tttgacacga ttccgactga tattgaagaa
     6301 gcagcaatga tcgatggtct tagccggatc cagatcatta ttcggatcat tataccattg
     6361 gctatttctg gaatcatttc tgtcttcgtt tattgcttta tggtttcgtg gaatgattat
     6421 ttatttgctt cgattttcct gtcgacctcc gaaaaattta ctttaccaat cggtttgaac
     6481 acattattca gtacaccaga ctacatttgg ggacgaatga tggccgcttc attggtgacc
     6541 gctttaccag tcatcattat gtatgcgtta tctgaacgat ttattaaagg tgatttagtc
     6601 gaaggcggcg taaaaggata aaaagaaaag aaagaggaaa gaaaagatga aaaaattagt
     6661 tgcattagaa ccaagaatag ccggattcaa agattacgaa gaccgtgaga tcgaaaagaa
     6721 cgaagtacgt atccgagtaa ttttcgctac tccaaaacca ggaacagaaa ttgtcgactt
     6781 tagaggatta agtccattta tcgatgaaga attttcagaa gaatggcgcc tattcttgcc
     6841 aagagaagaa ggaagccaac gcggtgtggt atttggcgaa tttcaattag gaaatatgat
     6901 tgtcggtacg atcgaagaaa cgggcagttt ggtgacagag tatcaaaaag gagagttagt
     6961 ctgcacttat ggacccattc gtgaaacgct gattacaaac ggtgttgata attataaact
     7021 acgtaaactt gatcggttgg atcaatggaa aaatgcagta tgttacgatc ctgctcaatt
     7081 cgctttaggt ggtatccggg aagcacatgt tcgagcaggt gatgctgtag caatcttcgg
     7141 tttaggcgct atcgggcaaa ttgcgattca attagctaaa aaagcaggtg ctactaccat
     7201 cgttgggatc gatcccatag cgcagcgtag agaattagca gaaaaatacg gtgccgatta
     7261 cacgctagat cctatgaaag cagatgtcgg ttttcgtcta aaggaattga cagataaacg
     7321 tggagtagat tcagttattg aaacgagtgg cagtgctgcc gcattacaag cttctttaag
     7381 agggatcggc tatggcggga tcatttctta tgttgccttt gctaaacctt ttccagctgg
     7441 gtttaatctt ggtcgcgaag cacatttcaa taatgcaaaa atcgtctttg ctcgagcatc
     7501 tagtgaacca aacccagatt acccaagatg gaaccgtaaa cgaatcgagg acacatgttg
     7561 ggaattattg atgaacggtt acttggattg tgaagaaatc attgatccag ttgtgccatt
     7621 tgcagaaagc gcagaagctt acatgcgtta tgtagaccag cacccagaag aaagtatcaa
     7681 aatgggcgtt caagtccgag gaggagaaca atgaaattcg gtacgcaaga tcaagcattt
     7741 ttcccaagag acattttaga aaaatttaga tttatcaaag actgtggatt tgaagaatac
     7801 gaaatcgacg gtaagttgtt aatagaaaat atcgacacag tcaaacaggc aatcaaagaa
     7861 acagggatcc cagtagtgac agcttgtaac ggctacgatg ggtggatcgg tgactttatc
     7921 gaagaacggc gtcttaatgg tgtacgtcaa attgctgaga ttttgcgtgc actaagcgag
     7981 gttggaggca aaggaatcgt tatcccagca gcttggggaa tgttcactta tcgattaccg
     8041 ccgatggttt caccccgaag caaagaagga gactataaag cagtcagcga atcacttcgt
     8101 tatttagata aagtagccga agaaacaggt acggttattt atttggagcc tttgaaccga
     8161 taccaagacc atatgatcaa tttgttagcg gatgcgcgtc attacattga ctctaatcaa
     8221 ttgaaacatg tgcagatcat cgctgatttt tatcatatga atattgaaga agatgatatt
     8281 tcaaaagcat tgactacgca aaaagagtat gtgggtcata tccatttagc agataatcat
     8341 cgctaccaac caggatctgg cgcaattgat tttactcgcc attttcaaac gctacttcaa
     8401 aatgagtatg aaggttatat ggtatatgaa tgtcgtttgc gaggagaaaa tgaagtgaaa
     8461 atttatcagg aatcattgca atacttgaaa aactgtctgg aaacagcgcg tcatttaaat
     8521 gatttagaga aagaatagag aaagaataac aataagtaaa taagcaaagg agggagaaat
     8581 aaatgaagaa agtagcaatc ataggcgctg gacaagtagc agaaaaagta catgctgctt
     8641 attatcaaaa aagatcagat gtcttagaat tggccgctgt ggttgatcca gtcagccagc
     8701 gagcagaaga cttttgccga cgtacaggat tttctcatgc gtatacatca gttacacaaa
     8761 tgctgaatga gatttctccc gatatcgtga gtgtctgtac acccaatcgc tttcacttcg
     8821 aacatgtatt ggctgccctc gaagctaatg cttcagtgat gtgcgagaaa ccaccagcta
     8881 tgacagctga tcaagcaaga aagatggcag agacagccga taaaaacggg cagattttgg
     8941 cttatggatt ccagcatcgt ttttctgacg aagcaacaat gatcaagaat catcttgatg
     9001 ttctcgggga tatttattac gtagaagcga atgcgcttcg tcggtctggt gttccaggct
     9061 ggggaacgtt tatcgacaaa gaacttcaag gaggcgggcc tttgatcgat tatggcattc
     9121 acatgcttga tacagcgatg tatattactg gatttcctaa agtaaaaagc gttcgggcgt
     9181 acagcttcca aaaaattggt acgaaaaaaa attcagggaa attcgggaaa tgggatccaa
     9241 gcaaattcac agtagaagat gcaatttttg gcacactgga attagaaaac ggcaagatca
     9301 tccaactgaa tacttcgttt gctttgaata tcagagaaga caaactgaat gtggcttttt
     9361 gtggcgatca ggcaggtgct aatctgtatc caggcgaact attcgaagat gtagatggaa
     9421 agctggtttc cttacaaaag accacgccag ttaatgaccc acatgaacta agtatggctc
     9481 attttgtgaa tcgggcgctc ggtgaccaga ctcagatgat tgcagatggc tggcaaggat
     9541 atgtcatcca gaatctggtg gaaaatttat atcgttcggc agaaacagga gaagttatta
     9601 ccttatgaaa gtagaagaaa aacagtttga tttagagtat atcaataagt atgcgagtca
     9661 gatgaccatt ggaaatggat tttttggtat cagaggaagc caggaggaag actatcggaa
     9721 tcaagtaagg ggaatgtttg cagctggtgt atataaccga cctttaggag cagaatcagc
     9781 cgagttagtg aatctcccag atgtcatcag atgggaaata aaaatcgacg gtgaacattt
     9841 ttctcttgag acgatgaaag ttcttgcata cgagcgggca ctagatttag aaatgggaga
     9901 gcttaaacgc tatgcgctca ttgaaactgc tcaaggaaaa aaagtggaag tcattacttg
     9961 tcgtgttgct gatcaaaata atctccatca ggcagccctg aaagtggtca tcaagccgtt
    10021 gaccgaagac tgccaagtag atgtgaaaac gggtatcgat ggacagcaaa ccaattttgg
    10081 tacacagcag ttggttgaaa aagaactgcg agtatttggt gacgatttga tcagtgcatc
    10141 ctaccagaca acagaaagta aaattgatat tgggattgca gctaaattca ataaagcagg
    10201 tgtattccaa gcaaaaaatc gccggatcac cgcttccttt tcagaaaaga tcagaaaaaa
    10261 tacgtccttt acgctggaga agatcatcgt gctctctacc tctcttcagc aaacagaccc
    10321 gtttaagaaa agtattgccc aagcccagaa aagcgttagt tatacagaaa ttttagagca
    10381 gtcgatgtct tattggacag atttttggca tcagcatcgg ataacgatcc aaggcgattc
    10441 ttctttcgat cagttagcga tggacttcgc tgcttaccat ttacagatta tgactccccg
    10501 caatgatcca agtgttagtg taggggcgaa gggactaact ggcgaaggtt ataaagggca
    10561 tgttttctgg gatacggaaa tttttatcct gcctttcttt ttatatcagt ttcctgaagt
    10621 agcaaagaat ttgctgctgt accgttatga tcgattagaa ggcgcacggc aaaaagcaag
    10681 agattatcac tatcaaggtg cacttttccc atgggaaagc gcacacactg gtcaggaaga
    10741 gacacccaaa tttgctgcat tgaatatcaa aaccggaaca agacaagaag ttgcttcagc
    10801 tgccgcagaa catcatatca gtgcagacct tgcttatgcc gttcaagagt atgtccaagc
    10861 aacagaggat caatcgtttt tagaaacaaa gggaaaaacg ctcattcatg aaacagcagc
    10921 tttttggctg agcagagctg taaaagtcga tgatcattta gagatacaca gagtcattgg
    10981 tccagatgaa tacacagaat atatcgacaa taatgcatat acgaactatc tggcgcatta
    11041 taatgtggcc ttagctgtta aatatcaagc tggcgatccg ctttttttga aagaatgtca
    11101 aaatttttta gataagttat atttgccagt gcctaacaaa gaacagctga ttccgcaaga
    11161 tgacacgtat ctcagtaagc cagttattga tttgaccact tacaaagaac gtcctggcag
    11221 ccagtctatt ttacttgatt atgcccgtag cgaagtaaat gaactccaag tcagcaagca
    11281 agcagatctt gtcatgctgt tatacttatt acctgagtat ttttctcaac caatcgtttc
    11341 taagaatttg cattactatg aaaacaaaac aatccacgac tcatcgttga gtaaagcgat
    11401 tcacgccata gaggcttgcc gtatccaaga aatggaatgg gcgtatcatt tattccaaga
    11461 agcgtgcctg attgatttag gaccagatcc gcattcatca gatgacggaa ttcatgctgc
    11521 ttcattagga gcactatggc tagcagttat atttggtttt tcaggtatta cagtaacgaa
    11581 tggaactctt tctatttctc caagacttcc ttcagcatgg gaaaaaattt cttttccttt
    11641 tatttggaaa aatcacaggt tgctatttgt tttgacaaaa aaacaaatag taataaaaaa
    11701 agagacgaaa gaaacattac ctatttttgt caatggagac caataccagc tggaaaaaga
    11761 actgatcttg gaacggaggg gaaaataatg cgtattctga ttgcgccaga ttctttcaaa
    11821 gaaagtctga cagccttaga ggcagcacaa gccattcaat caggttttca agaagttttt
    11881 gttcaggctg aatatgatct gctacctata ggcgatggag gagaaggaac ggtttcatca
    11941 ttagcagaca gtttaaggtt gaagcggcaa acagcagtcg ttactaatgc atttggcaaa
    12001 aacaaagaaa tccagtacgc ttcagacggt caaactgcga tttttgaaat ggcagaaatc
    12061 gtcggattga aagatgtccc actaaaccaa caacagccac ttaggttatc cacgcgagga
    12121 gtaggagaac tgatacgaca attgttgcaa caaggtgttc gacacatcat catcggcgtt
    12181 ggcggaagta gtaccaacga tggcggcatc ggcatggcag aaggtctagg ttatgctttt
    12241 tacgatcaag ataatcaaaa agtagaagca ctgggagaaa atctaggtaa aattgctaca
    12301 gttgataaaa cgaacgtgcc tattgagctg attaaagcca aaatcactgt cattactgat
    12361 gttactaatc ttttatgcgg aaaagaaggc gcttcttttg ttttcggacc acaaaaaggc
    12421 ttgaaagaaa atgaattagc agtggctgac caagccatgc atgattttta tatgagattt
    12481 gcccctcaag taatcgaggc atcgggcagt agtgctggtg gtggaatggg tgcaggactg
    12541 ttagcatttg cttcagggaa aatagtctct ggcatcgact acgtattgga tttactggag
    12601 ttcgataaac gggtacagca agcagacctc gtaatcgtag gcgaaggacg tctagatgga
    12661 caaagtctaa aaggaaaagc gccagttggg attgctagaa gagtaccaga gggcgttcct
    12721 gtgatagcta tttgcggaag tgttggtaaa ggaagcgaaa aagcagcaga tgttggcatt
    12781 tcggccgttt ttccgatcct tcctgcagct gtgcctttac aagaagcttt acagcaagcc
    12841 actgataatc ttcatcgcac agctaaaaac atcgcatctt tacttcaagt cgttcatttt
    12901 caaggaggta atcgatgaaa atcaaagcag cactattcga tttagatggc gttcttgtag
    12961 acacagctcg ttatcattac gaagcgtggc tggttttagc taaccagctg tccattccat
    13021 tcactgagaa agaaaacgag caactgaaag gaatatccag aaccgagtca ctagagcgat
    13081 tgctgtcatt tggaaaaatg gaacagaaat tctcagaaaa agagaaatca gcctttgctg
    13141 agcaaaaaaa caatctatat cttcaagcga ttcaaaaaat ggatgaaaca tccgttttgc
    13201 ctggtgcaat agctgttctt gagtacttga aaaaaacaaa catcaagatc ggattgggat
    13261 cagcaagcaa aaatgcacgc ctcattttag aaaaaacgaa tttgacaagt tattttgatg
    13321 tccttattga cggcacgcaa gtatcaaaag caaaacctga tccagaagtt tttctcaaag
    13381 gagcacagca attgaatgta ccgccaaatg catgcttggt cattgaagat tcagaagctg
    13441 gttgtcaagc agctttggct ggtaacatgc atgtattggg aataggtgaa aacataaatc
    13501 ttccttctgc tgaatatgtg attcctgatt taaccgtttt tgatcaagta cgttcgtttt
    13561 ggcatttatc agaagccgta tcacgtatat aatagtttcc gttttttctg tcacaagcaa
    13621 aacgttatcc tccttttgga aatcaaggta tgatgagaaa aaagggggat tttatatgac
    13681 aaaaaaagca gttttcgcag gcggatgctt ttggtgtatg gtcaaaccat ttgaagagca
    13741 gccagggatc atttctgtga catccggtta tactggcggg catgtaccaa atccaactta
    13801 tgaacaagta acaacaggca caacagggca tacagaagca gtagagatcg aatatgatcc
    13861 agaagttatt tcttatgatc agttggtgga aatctattgg cagcaaaccg atccgactga
    13921 cgcattagga cagttcgctg atcgcggtga ttcttatcgt ccagtgatct actataatga
    13981 tgaagaagaa cgaaatattg cagagcgttc aaaacaagca ttgcaagaca gcgggcgatt
    14041 cgataagcca atcgtgacaa aaatcgaacc aagaagcgaa ttttatcctg cagaagatta
    14101 ccatcaggat tattacaaga aaaacaagct ccattacaat ctttatcgag aaggttctgg
    14161 acgtgcaggt ttccttcgtc gacattgggg cgtcaagaaa taagaagccg aaaaagctac
    14221 ttatggaaca ccgtatatca gaggttgtaa aaccagcatt ttgagctaat actgagggtc
    14281 agctgattga acaccattta gtggagaggt tgtgaacctg tcgcctagct ccaagtcaca
    14341 aggatttggt aagagaacac tatatatcag aggttgtgac aagtcgtttg tcacaacctt
    14401 ttttcaatac ctaagatgaa ggacaatcag caacgcactg ctcaaaaaag gaatgaaagg
    14461 cagcttttcc aacttttttt tatgacagat aaagtaaaca gaaaaaacgg atattccaaa
    14521 cagactagca agaaacaaga tctgaagcaa ctcttctaga gacaagccac cactccaaga
    14581 aagcatcaac agagtatcgc cagctccgag ataggattga ccatagcgaa ggcacacacc
    14641 gcctacgata aacgttaaca gcacactttg ccattggaag acgccggcct gcaaccaaaa
    14701 aatccataaa attgcccaag aacagaatag cgtgtgagaa tggaccatga agtaaaataa
    14761 atccatcaac gaaaatagca aagccgaaag catccatatg aacgtcgaaa aatttcttgc
    14821 ggaagaaaaa gtaaagaacc aggcaaaaag accaccacat aaggcttctg ccaacaaata
    14881 gtagaagggc aaccgacacc tgcaatagcg gcagcgaaat cgttggacga tgatcgagag
    14941 aatcggaatc aattcatacc aagaaagcga acgacagcat ttgacacaat gagatctagg
    15001 ataaataata gagtgaccta acggaatccg ctgggcggtt aaacataaaa atgatccgat
    15061 acagcaacca atgataaaat aaagtaacac gccatctccc ccttttcaaa accaagatac
    15121 gcaagcttaa actaaatttt tgtacataca taaaatttga ggtgaaaaaa tgaacgtttc
    15181 aatcggatta gatatcggaa ccacacaaac caaagcagtt gctttcacag atgaagccca
    15241 agaagcagca tccgcttatt ttcgctaccc tttgatccaa gaaacacaag ggatggcaga
    15301 acaagatcag gaattgatct ttcaaggtgt ctgcaccgtt ataaaagaag taattaaaca
    15361 gttgccttca gacacaatca ttcaatttgt ctccttctcc tctgcgatgc atggcctgat
    15421 attgttggat gaagagaaaa caccgttatc tcgaatgatc acttgggcag ataaccgagc
    15481 agcggacgaa gcagaaaaac tgaaatatac cgtgcttggc aaagaatttt atcggcagac
    15541 tgggacgccg attcatccaa tgacaccatt gaacaaaatc aagtggctgc aaaacagcca
    15601 acctgaatta ttcaaaaaaa ctgcttattt tgtcggtatc aaagactata tcttttatag
    15661 attgtttggc gaactgatca gcgactacgg aacagcttca ggaactggat attttgatat
    15721 ccatgaattc caatgggcag aagaagtgct tgactacttg aacattcgaa tagagcaatt
    15781 gccgcaagtc tttccttcca cctatcaagt aacaggtcta ccatcaaaaa cagcagcaga
    15841 actaggtttg ccaaatgata tatcatttgt tttgggaggg gcagacgggc cgctttctaa
    15901 tctagggtta ggtgcttttg gtgatacgat cgccgctttg accgtaggaa cgagcggagc
    15961 acttcgtttt attactactc agcctcagtt ccatccagaa atggagacgt tttgttatgt
    16021 tctggatcag aaacattggg tagtgggagg agcgactagc aatggtgctg gcatctttga
    16081 ttgggcaggg catacatttt tacaggaagt cgttcaatct gctaaagaaa aaggagaaaa
    16141 tccatatgat gcactgcttt cggctatcgc ttttgtgcca gcgggtgcca atggtctgat
    16201 ttttcatccc tatttacttg gtgagcgagc accgctttgg gacgcagatg cgtttggcag
    16261 ttttatcggt ttacagcggc aacatacaga aaaagaaatg atgaaggcga ttctagaagg
    16321 gatctgtctc aacctttatc ggattttaaa aggagtttgc caggaaaatc agctcacaga
    16381 gatccgtgct acaggcgggt ttgctcaatc tccgattttt cgtcagatga tggctgatgt
    16441 actgggttac ccgcttgttt tccctactgt cacagaagca tccgcattag gagctgtgat
    16501 tttaggctgg agaagcatcg gaaagatcca aagtatcagt gaagcacagg aattgataga
    16561 attgaaagag caagttggag tacaagaaac tgatcataaa aaatatcaga aaatctatcc
    16621 attatttgtt tcgacacaaa agcatttagc acagacttat cagcaatttg ctcaactgcg
    16681 agaagaatta gctgattctg aaaaatgatt ggctttacgg ataaaaaagc gaactgaaaa
    16741 tcgttctcag gtaaatgatt ttgttgtact tttcattgaa agtctactat tattttaagt
    16801 gtaagaaaat gttaggaggc aagaaagatg aaattccaac aaaccaaaga agtattaaat
    16861 caattagttg cagatctaag ccaaatgtct gttgttgttc accaaacaca ttggtacatg
    16921 agaggaccag gtttccttac attgcaccca atgatggatg agttcatgga tgatttgaac
    16981 gctcagttag atgaagtatc cgaacgcttg atcacattag acggttcacc ttattctaca
    17041 ttgcgtgaat ttgcagataa cacaaaaatc cctgatgaaa ttggccgctg ggatcgtact
    17101 attccagaac gtttggaaac acttgttgca ggctatcgtt atctagcaga tctttaccaa
    17161 aaaggaatcg aagtttctgg agaagaaggc gacgatccaa cacaagatat ctttatcgaa
    17221 ttcaaaacag ccacagaaaa acgtatctgg atgatccaag ctcatttggg caaagcgcca
    17281 gagatcgacg cgtaaaaact gttgttgaac ttgaccg
//
LOCUS       DBNICI010000048        17246 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.048, whole genome shotgun sequence.
ACCESSION   DBNICI010000048 DBNICI010000000
VERSION     DBNICI010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.048"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(361..987)
                     /locus_tag="AC1ZCC_001303"
     CDS             complement(361..987)
                     /locus_tag="AC1ZCC_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629567.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1282..1617)
                     /locus_tag="AC1ZCC_001304"
     CDS             complement(1282..1617)
                     /locus_tag="AC1ZCC_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HIQ1629568.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1604..1888)
                     /locus_tag="AC1ZCC_001305"
     CDS             complement(1604..1888)
                     /locus_tag="AC1ZCC_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HIQ1629569.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1944..3467)
                     /locus_tag="AC1ZCC_001306"
     CDS             complement(1944..3467)
                     /locus_tag="AC1ZCC_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HIQ1629570.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3460..4635)
                     /locus_tag="AC1ZCC_001307"
     CDS             complement(3460..4635)
                     /locus_tag="AC1ZCC_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HIQ1629571.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4639..4824)
                     /locus_tag="AC1ZCC_001308"
     CDS             complement(4639..4824)
                     /locus_tag="AC1ZCC_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629572.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4790..6484)
                     /locus_tag="AC1ZCC_001309"
     CDS             complement(4790..6484)
                     /locus_tag="AC1ZCC_001309"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140657 - ATP-dependent activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HIQ1629573.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6481..6954)
                     /locus_tag="AC1ZCC_001310"
     CDS             complement(6481..6954)
                     /locus_tag="AC1ZCC_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HIQ1629574.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(7023..7178)
                     /locus_tag="AC1ZCC_001311"
     CDS             complement(7023..7178)
                     /locus_tag="AC1ZCC_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HIQ1629575.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7312..7692)
                     /locus_tag="AC1ZCC_001312"
     CDS             complement(7312..7692)
                     /locus_tag="AC1ZCC_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIQ1629576.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7696..7911)
                     /locus_tag="AC1ZCC_001313"
     CDS             complement(7696..7911)
                     /locus_tag="AC1ZCC_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629577.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7914..8321)
                     /locus_tag="AC1ZCC_001314"
     CDS             complement(7914..8321)
                     /locus_tag="AC1ZCC_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HIQ1629578.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8587..10062)
                     /locus_tag="AC1ZCC_001315"
     CDS             complement(8587..10062)
                     /locus_tag="AC1ZCC_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HIQ1629579.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(10052..10900)
                     /locus_tag="AC1ZCC_001316"
     CDS             complement(10052..10900)
                     /locus_tag="AC1ZCC_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HIQ1629580.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10937..11299)
                     /locus_tag="AC1ZCC_001317"
     CDS             complement(10937..11299)
                     /locus_tag="AC1ZCC_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629581.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11300..11458)
                     /locus_tag="AC1ZCC_001318"
     CDS             complement(11300..11458)
                     /locus_tag="AC1ZCC_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629582.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(11599..12223)
                     /locus_tag="AC1ZCC_001319"
                     /pseudo
     CDS             complement(11599..12223)
                     /locus_tag="AC1ZCC_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12220..12531)
                     /locus_tag="AC1ZCC_001320"
     CDS             complement(12220..12531)
                     /locus_tag="AC1ZCC_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629583.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12575..12868)
                     /locus_tag="AC1ZCC_001321"
     CDS             complement(12575..12868)
                     /locus_tag="AC1ZCC_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629584.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            13062..13709
                     /locus_tag="AC1ZCC_001322"
     CDS             13062..13709
                     /locus_tag="AC1ZCC_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1629585.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13770..14915
                     /locus_tag="AC1ZCC_001323"
     CDS             13770..14915
                     /locus_tag="AC1ZCC_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIQ1629586.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(14996..15301)
                     /locus_tag="AC1ZCC_001324"
     CDS             complement(14996..15301)
                     /locus_tag="AC1ZCC_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HIQ1629587.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(15326..15757)
                     /gene="ruvX"
                     /locus_tag="AC1ZCC_001325"
     CDS             complement(15326..15757)
                     /gene="ruvX"
                     /locus_tag="AC1ZCC_001325"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIQ1629588.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(15754..16020)
                     /locus_tag="AC1ZCC_001326"
     CDS             complement(15754..16020)
                     /locus_tag="AC1ZCC_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HIQ1629589.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(16275..17000)
                     /locus_tag="AC1ZCC_001327"
     CDS             complement(16275..17000)
                     /locus_tag="AC1ZCC_001327"
                     /EC_number="3.1.30.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HIQ1629590.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
BASE COUNT         4933 a         3572 c         2743 g         5998 t
ORIGIN      
        1 ctgtgaagtc aacgttttga cctgattatt agagaaacaa atggcaaaat agttcttcat
       61 atttcgccgt ttttcgagcg aatgccgagg ggcagctgat tgaacatcgt ttatttggaa
      121 aaagggattg tgacaagacg tttgtcacaa tccctttttt tatatcttat taattcattc
      181 ttttgttcta ttcttcttct tccgccatga atgagaaacc catatcaaag aactataaag
      241 gttatcaaaa cagcgtttat gactaaatga attagagtta tctatcacta tttttcaagc
      301 gatttaaacc aaatggcata caatatggca tacaaaaact tcaataagcc gtgggggttt
      361 ttaaatttct tcttgttctt taaaatcaat gagtggagct acataatttg aatccacttt
      421 ttcaaacgtc actttttgca tcgacgaatg ttttctttcc caagataacg taaatagatt
      481 tgactctgga cttataaagt ctacttcacc ttctagtatt actttcttat gcaaatattc
      541 ctctaataaa tatagacttg ctaaagcata caacacattt tccaaatttg cttccttata
      601 atttgaagaa cgaccatgtt ttactctgtt atatattttc caccaatctg gtgtatcttc
      661 aggccaccca tctccaaaag gttttagtga agtcaatgtt aaagaattca taaccctaac
      721 accattatcc aaacacatta attcgctaga tttaataaca gctttaaaat cagttatgct
      781 tttattgcta ctgctatcaa atccgcaaag ttctttgaaa ataatctcac actccgaacc
      841 aatcgctagt agtagttttt gatattcaat agagaatgtt ttgaaattat tctcatcgag
      901 catcacaaaa tctgtggtat taataaactc attttctaaa tgtagataat acttccaata
      961 aaaatttgta aactctgctt tgtccattaa atcacctctt ttgaattata acaaaaaaaa
     1021 cacgtccaca acagacgtgt tctttgattt ctacacaatc agcttgccaa aaccatccaa
     1081 tcagtcctag catgcctgta ttaaaattga acgttcatca tctatcatct ttcttagtcc
     1141 agcatgtttg caaccacacc tacaattttc agtccaacaa tagatttcga ctagctaagt
     1201 cttgcaaaat tcaggcagct aacccttcat ttactacctt aattatacta aatacccaca
     1261 acgtttacaa gtgtaatcat tctaagaaat ctgtttagct atgatcgttg tataatcttt
     1321 aaacgccgtt ccttcattga ttgaaataat tttataactc ttgtttttcc aagctacttt
     1381 catatcgttt gttagttcat aatcttgatc gtatcgaata ataaacgtca acgttccttc
     1441 aagaatggta cctacactag caagcacgtc ttttagcatt tgtgaacgca cattagccca
     1501 acattcaaat acagtttctt cactatccac catctgccca tcttcatctt ttacccgttt
     1561 tgtcttttta aacttgactc tctcagtcaa ttcatttaca ttcttaatga ttggcataat
     1621 aagcacctct catttgttgt actaatgctt gtaccgttac tggtatccgt tcgctgctgg
     1681 cttcttgacg gttcaaatac caatgttgag taagtaatga caccgccaaa tcaaattgct
     1741 gataattatc catatcttta tccgttgcgc tactatcaat agcacttttt atatattcgc
     1801 ctgctgtatc aatcaattgt tgaataaagt catcatcaat agtgtgatcg atccgcatac
     1861 ttttttttat gctatccaaa attaccattt tatctctcct aatgaaaaaa aggcagtggg
     1921 ataaaccgcc gcccaatatt tttctatact tttggtgtta caactggcgt aaactcaata
     1981 tacactgaag cattttgatc aattttctta taatcgttgc gaacgatcac tgcaagtcct
     2041 tgtgagtagt aatcgaattt ttcccattga gtcgttactt gatttctacg agcaacaaag
     2101 atagattgtg caatatcccc cataatcata gggaatgttc cagctttagg attggcaaac
     2161 aatgtatctg caatcaatac tactggcata ccgaataatg atttaccact aggtgctgtc
     2221 acatcgggtt gtaaaatgta acgtccatct gaatctttca atgtatctag gtggtaatat
     2281 ccgctttggt ttagaattac cattttattt aatgctgggt ctaatgccac attgtatagt
     2341 tgtttcaaat cgtccaacgt agcagccgtt ttcttagtga atgtttttaa taaatccatg
     2401 atatgcttat tgtcagtgtt ttctaccaat ttcgctaact gatctttgac ctcttgtaca
     2461 atattcactg ctgaatcttc cacaacctca tttgataagg caatcttacc agcacgagtt
     2521 tctactttat aatcaactga agtaaacatt tcagcgtcaa tatcaccaat ttcagcaagt
     2581 tcagcttttg ttgctaacac tgcttgttgg ttagttgcta ctggatattt accttgtcca
     2641 tttgatactg tttttactgt tgcatattga gctaagttat aatttgaacg tttcaaatca
     2701 aagacttcac cgattacttc ttcgggaaca actgccgctg catttacagt agtgactcca
     2761 tcacgcactt cgccttgtga acggatatat tcttcatacc cacgcaattc tgttttttcg
     2821 ttatcgatta aagttttttc catgttatta ctctcctttt gtgctttaga agtggattct
     2881 aagctattgt ttgtttgttt tgcttggttg ttactcataa acgattcgta agaacgctta
     2941 tttacttgga catttgatga atcataagcg ggaacggtca cgacactaat ttcattaagt
     3001 gctttgattt tatctattga tcgttcaatt gtgccatctt cttttttgtc gaatgaatct
     3061 aatccaagca cgaacccaaa ggacatagaa tcaacaaccc cttttgagat attctcatac
     3121 acatcatttg aataggtcgt gtcggttagc tctgcttcaa aatgtaatcc tgtatcatcg
     3181 acattcaatt tcaacgtacc tgctttgaca ctagctagtg gctttgaata atcgtgtcca
     3241 tataataaga acacatttga taaatccacc tcttttaatg cttcgggtgt aatcacttct
     3301 acaaagccgc ctaaatcttt tgacggttgc ccgaatttca acgcatagcc tgaaatagtt
     3361 ttgccgtctg ttggttgttt ttcattttct ttctctactt tgggagaatc ggctgttagc
     3421 tcagcttctt ctgtcagccg tttttccttt tcttcttcat tattcaactg gtgtaactcc
     3481 ttcctgttct tttaattgat atttctccaa cgtatctaaa tacgtaaagt tcaaacttgc
     3541 tagtaaacga tcgccaccat ccatgggggg tagccccatt ttcgaccgcc cctcattaat
     3601 cgtgagaagt gaccctttga cctgatccaa tacatttttt acttttgttt caggatcagt
     3661 ctctagtaac tgatcggaat taaagcggat attggtttca agtttcgtat ctaattcact
     3721 cacaaacaca ttaaagtaat gaatcagcgt actttggata tactggagat tactttgaac
     3781 ggtgctagaa tgttcatttt caacgcctaa acgctcaatc ggtacaccaa acgccttggc
     3841 aatttgtttt gtgttccaat cattggaatt gataagcttc aaaacatcgg tgtttatttc
     3901 taatgttttg tagtccattg tttcatcaag aatgatcgtt ctaagagcat tatctccact
     3961 acttgaaccg ttggcttctt caaacttttc acgaattgcg ttctttgctg atccgtctaa
     4021 atctgactta tgaaccttca aaatacctga cccactgaca ccacgagtaa agaaattgtg
     4081 caatgtgcgg ttgccagctt gttgaatttt cagttcatca cgtaacgcat aaagtggtgg
     4141 taatcctgtt aaaccatctt gggtgaaata tttgaagtgc aaaatatcgc tagacttcac
     4201 acgtcttttt ttatcaccaa tctgataaga caacgtacca ttatctaatt gagtaaccgt
     4261 tacacttgag ttaggtaata agtgaatttc ttctaccttt tcaccgttgc gtttaatctc
     4321 tgcaaagcta ttaccgttta gcaacatatt gaccgccaaa gcaaacttta aatgccaacc
     4381 atccatttct gaattaggtt tctcgtttag taacttcacc agttcattat cagcttgtgg
     4441 catattgttt ttgaccaatt gaatcggact tgaagcaata tcactagcaa taattcgaac
     4501 ggctgcaaac acatcactat ttcttaatgc acgaacgctt gtaaagctgg tggtgtaatc
     4561 atccgattgg attgatacca catgttgtag gaaaggatct cccgttgtat tttgtctcgc
     4621 tttaaaaaat gccatctttt aacctccctt ctgttctgta tttagcatga ccgctacccc
     4681 aataagcact atgcccagcg ctaacaagcc atagtagaca tttgttagta aggtgatagc
     4741 aaccaacaca cacaccaatc caagaagtaa aagaacggta tgaatgtaat tagaaagaaa
     4801 attcttcact tgtataaaat tcattgtctg ctttcgctcc ttccgttgtg gtgaagtaat
     4861 ccattgcaaa cacataggca ttgattaatg cagctattgg atcaattttg ttactgttct
     4921 ttgatttatt aatctgaatg ccgttgttat ccgtttttaa aatagcgttg ttcactgcat
     4981 gggtgagaat cgtattttta ttgtgaataa cgttgccttc ataaacttgt tctctaaaag
     5041 ttcgggtcgg aacgtttaga gtaatcgttc cttgtcttac ttctaacatt gggtagttgg
     5101 ctttttcagc tttactgata agcgaattag cgttatacgg gtcataacaa atccccatac
     5161 attccaaatc attttcttga atcaaatctt gaataaactg aaatacttgg tcatagtcca
     5221 cgattccact ttctaattga gtaatggaac actcaccagc tttttctagt tctcgataag
     5281 gcaaaccgtc gcgcttttct ttgtcttgaa gcccatattt ggtggctaca aagctatgtg
     5341 aatcacaata aagttcgccg ttatctagcg gtacgatcca tgaaacgcta gttaaatcat
     5401 ctgttttaga taaatccact ccgatataaa cgggcttgcc tgtaatatct tgtggttcta
     5461 cttcggttgc ctgccagtct tcagcaatca tatagctgtc ctcactcgct tgtctccata
     5521 aattgaagtt tttcaccaat acagaattaa ggttattttg tttcaaagca aggttcacat
     5581 catcttgaat ggttggaatc attacttttt tgatttcttc actttcaaaa atcggattgg
     5641 ctttgatcca tttctcttga tcgtgaattt cttcgggatc gtccaattcc catatcgcta
     5701 taaagtacct gtcggcttgt tcttcgcctt ttagcacacg atctaacaat aagtattcct
     5761 cgtacatagg aacgtttaaa tcaagcccag cggtactaat aacacacaat aagccgttct
     5821 tttgttgggt cattcctgac ttaatgacgt tgtacgtttt acgagttttt gcttcgtgcc
     5881 actcgtcaag aattgcggtt gttggtgcaa acccatctag cgtgctggtt tcactagcca
     5941 gtgccatagc gaacgaatta gagggcaagt gctgaatctt agagttcata attttcagtt
     6001 gtggtcttaa aaacttacta gacttgacca cgtttctaag tgaattagac agcatatcat
     6061 agcccaattt agcttgtttc aaagcgttac ttacaaacaa tacttgcctt gcttctgctg
     6121 gttcttgttc cataatcaac gcattggcag ccatgccact tgcgatatac gttttcccat
     6181 tcttacggct catactggta aacgctcgat tgaatcgcct gtaattccct tctttagtac
     6241 gccagccata aagactgcct agaatgaatt tttgaaatcc taacatcttg attgctttac
     6301 cgtccgtaga tggtagcaac tcaatgaatt taatcgcttt ggtggcttgt tcttcatcga
     6361 acacataagg gaagctatct gacttagaac gctccaaatc gcttatatga cgctcacacg
     6421 cttggataat tttcttgggt gcaatcagct tacctgatag gactttctca atgtaatcat
     6481 tcatgaattc atcaactctt tgaacgggtc ttcgggttca tcatttttaa ccttgttcaa
     6541 agctagtttt gatcgtgatt ctaaggtcat acctagtgag gtggcttgtt ttttcaaatc
     6601 actcatagct tgtgattgaa cccgaaccgc tggattggct ttctttactc cgttttcgca
     6661 tgtgataaac gtacctgatt ttcttatctc ggcttcggct gttttcattc gagcaaaggc
     6721 taaacaatag gctaccaatg aaccataatc tgcttcactc aatggataat ctttttttaa
     6781 tgttggtacg atacgattcc attcacttct agcgctcgct gccatccagt cagggggcgt
     6841 gatatttatt aatggttgct gcttgaacaa ttcttctcta gcgtccacac ggattgcttt
     6901 ttcttcactt gatatattgc cttttgtatc gtgtaatatt ttagggtgtc ccatgttgtc
     6961 ctcctttcag acaaaagaaa aaggaacgta agcaattaag ctcacattcc ttcgtttttc
     7021 gatcagaatt tttctttatc taatttatct atataaatga tttttccgtt ctgcatttga
     7081 atggtcactt gaccaaaatg aggtaactca atggtgcgct gtttccctgt tttatcagta
     7141 ataacaattg tatttttcaa taaatctcct actttcatta tatacttttt cacttgtgat
     7201 tacaagtgta atcgttattt gtaattaccc ttatgccttt tcacaacggt tatttgtacc
     7261 aaaatgagta ggcatcgatt gtcagtaagt ttcacactat gcccccttaa ctcaaagggt
     7321 actcttttct cgtctttgtc tttcattggc tgtcttggaa ttatggtgtg catggcataa
     7381 agaacgaaaa ttagtttcat ctaaccgtct attccaatca tcttttagtt caattttgtg
     7441 atctacaaca tcagctttcc ttatcaagcc ttcttctagg caatcctcac atactggatg
     7501 atttaaccga tacaagtatg aagcctttcg ccatgttcta ctttgataga attgaaaata
     7561 tttaccttcg ctttgctttc tttcttcacg cttaattgcc gtttgctttg ctttatgctt
     7621 cccacaatac ttctgcctat aatcaatcaa tgttttacat ccagcatgat tacattgttt
     7681 tttaactgcc atagattacc aaccaaagcg atctcgttcg tcagaatcat cttgttccca
     7741 ttttgatttg tattcaatat aaggcgaaac tatcttcaag cacgttccgt ctttaaaatt
     7801 aactttagtg attttatctt cctcaaaact aataaagtct acatcttctt gagtgtttga
     7861 atactcaatg tgtttctcac catcgaagta tctaatactt tccatatgca taattattct
     7921 acctccaata aattagttat caattcatca tctgttagta gcaaagccaa gaacttatca
     7981 tggatgttta aaaacaaatt atcctcattc gatgtaagct ctcgttgtct cgctcgatca
     8041 tataggttaa gcagacaatt aaattcatcc attagctcac ctgtatcatc atagttgcca
     8101 ctatctacca atgatcccac tactagcgat tgacaccacg ctactgactc ctgcagccaa
     8161 tcaggttctt cttccattat ctttaatact tctggatcaa atgtggaata atcttctgtt
     8221 accaatccca cactaaatgc cagcgtgttc attagaaacc gatccatttc atctgtatat
     8281 tgaaagttcg gtgctttgtt tgttgaaata atattgagca tttactttcc tccttgattc
     8341 gtaacacgtg gtaacaccta cgtaacaggt acccgttacc actcaatccc aacggggaca
     8401 agggttctag cgttttggta acaggtaacg ggtaaaatcc agtaacatta ttccccagca
     8461 tattagctaa ctatttcaaa agttttaagg aaataaggtg ttaccccgtt accaaattgc
     8521 tgtatccctt ggggcagtaa gggtaaacta gtaacgggct aggtgttact gacccgttac
     8581 tacccgttac tttttgtact ttccagctcg atttttattt tcataggcat ttgacttatt
     8641 gtaaaatcct cgtcgtttat ttctttttgg cagttgcttc cgttcccaac caggaatact
     8701 gctaataatc aaggcaatct ttttagtttc tgcatctgat cgaccattca ataaatcctt
     8761 tgcttgttta tcaaaaacag cttccaaaat atcagcggtg agtacgctat caactaaata
     8821 aaactcttgt tgcatagtag ctccattata gaaatgctgt aaatttttct cattacattt
     8881 actgtcagga tagttctgat aataagaacg tctcgcccac gctggagctt cttcccattt
     8941 atacgggatt tccatttcca aatatttagt gattgcttct ttaattggat tctccatctt
     9001 ggcatcctct tgatagtctt ttgcaatttc taagacttct ttatcttcat cgggactaaa
     9061 atagattcgt tgtcctttat caaaaagcac ctttgcttca gctaacactt gaagaaaata
     9121 ttcatccttt gttttaaacg gattcatctt tggcttattc ttacaaggca aaggataaaa
     9181 acggcggttt cctgtatcat ctttcaaata ctgcccatca ttcgacgttc caataaatac
     9241 acattgcctt gcccaagcta tgacattccg accataagga ggtctaattt tatcgatctt
     9301 ggcactgatg aagtttttta tctgttcaat tttagttttg ttcattgaag ataattctcc
     9361 aagttcaata attacattgc cttgaagctg catataatca tctttctttt cacctagcga
     9421 atctaatgaa tcggtgaaaa aatcatccgt gtaaagtaat gctggaacgg tactcttacc
     9481 aatcccttgc ggtccatcca aaactggaac gatctcaaac tttattcctg gtttatagat
     9541 acgtgcgact gatccgacta tccaacgttt tgttacttct cgtgtgtaaa ggttgtcgtc
     9601 tacccctaaa aggtcaataa acagtgtttc cagtctaggc tgttgatccc atttaacttt
     9661 ttcgatacgt tcttttactg ggttgaaaac attctttcta gccacattta ctactgcttt
     9721 gtgtattttg ttgtcatcaa aaactacatt gtagttttct tcaatatatg ccgttaaggc
     9781 atcctcaaaa ctgtcttccc attctccagc ggtacgattg atccaaggag aatcttttaa
     9841 taggtgaatg tatccgctaa attcgttata agcaattgtt ccagctaaat tgctatcatt
     9901 ggttaaaatc aattctgcat tcacaacact gttactttta atctgatcgt ctttattgca
     9961 tcttaatcgt gacatccagg ttttcaattc aatgatattg tcttcacgct cgctggcttc
    10021 gggaaagtct aaaatactat ctaatttatg cttatgagcc atgagctttc cccctcaaat
    10081 cttttttaat catcgatcga ataatttgat tcacttcttt atcttttagc ggtggttcat
    10141 tgtacgcatt tgctgtataa atcaattctg ctaaagtttg attatctaca ccgtgccata
    10201 gtaaccaacc gcataactta gttaaatgat cgtttctact accttcgcca gcgccttgaa
    10261 caatcatatc taattttttt gctgtccctg tttttgctac gggtcggtat tgagtcttta
    10321 tttcttgcgg tttttttagc agtaggttca ctagccaatc gggagctggt gcaatattat
    10381 ttagagatac gttttcaata ggttcatatg ctctcccaac acttcttgaa ataatcatag
    10441 ttggcgctat tggaactcct ttagtgataa tatcaatccc gcttaaactt ttacctttat
    10501 catcacgccc gaagatatca gcaaatgcac ctgtttcttg ttggataatt acaccatcag
    10561 gaattttaaa gaagaaatgg attcctttac ctggtgtgat ttccgtataa gtagaaggaa
    10621 atggatcata cattgaagca agttttttat aattctcaac gccgtttcgt gtttcagaat
    10681 gttgatctag gtcaactacc accatttgat gatcttcaag cattaaccca atatttctag
    10741 ttgcatcttc cgtccaccat tcattaattt gattagaatc gttagttgca tcattccaac
    10801 cactagagcc ttttattgga attttagttc caggagataa gggataaatt gaccatcccg
    10861 acttttgtaa ttccgttgct gcttgataat tatttgccaa taaacctctc ctcctttttc
    10921 aaaatactgg tataaatcat tcagacacct ccatgagctt caactgctca agttcgtatt
    10981 tcaacccatc ccgaacaaat tcagcacctt ttaattgatt ctgatacttt acccatgtat
    11041 taaatgtttt cacccttgct tcttgcatat cagtaatgtt taaccatgcg gttaaaatct
    11101 gattttttag ctcgttgatt tctcgacaaa ttattttgtg ttctgcatgt tttttgagta
    11161 catacaaaac tgttagtttt tcattgaatg aatttccaaa gtaggttttc ccgatattat
    11221 tttctagttc agcgatccct tgattaattt cctctaattc aaggctatcg agttggttta
    11281 atagttttgt tttgtacatc tacttatgcc ctccagttct taattgatag tcgtactcgt
    11341 cataagcagt gaagagatat aacgctaatc ctgcaaagat agctattttt gcaattaatg
    11401 gtaataagcc gaaaattccg ccgattaaaa tgcttcctat aattaagttt cgtttcataa
    11461 tggttccctc cgatttttta atatggtata atcggaacaa agaatatcgg gttttatccc
    11521 aattaagttg cctttatcct gtccgccaag actgttatgg ataaggcttt ttctttgttc
    11581 tcgatcatgt taatgctcct aagttgtctg caattcagca gaacgattat aaattttctc
    11641 actcactgaa aactgcaacc catatttttc tttaacagtc attaattcta ctgtctcatc
    11701 taaaataggt tcacgatcaa ccaacatttc aggagtcata tctttctttt ttaccatttt
    11761 ggaatgacca taaatagttg atacagcttt attggctatc acattcgctt taatgtagtc
    11821 tactggtttt ggttcagtaa tagcgtggct taatttagtc attgcttctt tttggtgttc
    11881 tttgtctagc atgcggaata cttgaaagcc ttcaagtcct gtggactggc gtagttcttt
    11941 gatgatgtcg aaaatccagt cttgaaatgc ctcggcttct tttttacggc ttttgaatgc
    12001 catgcggtaa atattttttt tcgttgatga tgtttacatc ttgtgttctg cccaatgaat
    12061 cgatgacctt actagtagta accccatctt taggcaaaga gtgaatggct ctagtaacct
    12121 gtttaagacc taacgcttcc gcaacatctt tcgccactgc ccaccattca tcattaatat
    12181 cgacaaatct tataatgtgt ccgttccaaa tttcagtttt cattcaacgt cctccagttc
    12241 aagccctagc aagctcgcta acaactcaac ttgctgatac aaaatttctt gatactcttc
    12301 aatagaaagt cgttctttaa actctgcttg tactaaatcg ttcaagccac gcaatactcc
    12361 gcttaccgtt ggttcttgtg gtagcaattt ttgatagtcc atttgttcgt acaaacattc
    12421 aaacgtttct tctagcgctt tgccttccaa gtttgctaat tctgccgctg tgtcttctgt
    12481 aagaattgtt gataatactt tttcgattgc tttattttgt gtcgattcca ttattatttc
    12541 ctcctattaa tccctttgcc cccacggctt gcgtttattt ttggtattct tccataaagc
    12601 gatcacattc atcttgtgaa attcttacga ctccatcaac tataatcaac tttaaaccgt
    12661 taggaacata tttctgcatc atagtattgt atgaaacgtt catatactta gcagcttgtg
    12721 ccttgttcat atattttgga aactggtact ttttttctaa taatgaaagt aacgttcctt
    12781 gagtttgctc tacttgttga acaactcgat cgtcaataat tttaaaagct ggatcatcaa
    12841 gcgtgagttt gtcaattttt aattccatat ttatcccccc tcgttagcat acatttctat
    12901 acaaagattt taaaaaaatg caagaggctc ttgaatacat ttctatacct cttataaact
    12961 caatatacca tcataccttt tcatttgcaa gaattttaac atactttttt gaacttttat
    13021 gtttcacaca tatttaaatc gtgatatagt agagaaaata tatgaatggt ggtgaaaatg
    13081 tggctagaaa aaaatacccc gaagcagtgg agacaggaca aagaattaga ctggctagaa
    13141 ttgaaaaggg actctcaatg gaacaacttg gaggtaaatt atccccaccg gccagtaaag
    13201 gtgccgttag taattgggaa aatggctata atcttcccaa taacaccagg cttaaacaac
    13261 tttcaaaggt attagatgta tccactacct atctattaaa cggatcttat acattaagag
    13321 atatgcatat gatgcccgaa attaagcgaa atgtgcttga aaagcaattg aaaaaaacta
    13381 ttgaaaaaaa taactctata attgattcta atctttctaa tgctatcaat aacatcgaag
    13441 aagataacct gactgtctca cagaagattt atttaacaaa cgcaataaaa tatcttaaaa
    13501 caggatctcg aattgaattt cctgacggga gtaccccaat tgcttatctc gcagctcttc
    13561 taaatacgct tttacgatat gactcattaa ttaatgattc agaaaaaaat acagatgaga
    13621 aaaaagagta tcaagaattt tatgaaaatg atcttctaaa agagttcaca gctcttgtta
    13681 ctgagctaaa taaacactat tcaaaataat aataaactgc ctcctccaac cgcccccacg
    13741 gcttgctaca ctatggaggg aaaactatta tggcaacatt caagcaatat gaaactaaaa
    13801 aaggtaaatt ttggctctat gaagcctatc ttggaatgaa caaaatgact ggcaaacctg
    13861 ataaggttcg tagacgaggt tacaaaacaa aaaaagaagc ccagcttgcc ttgtcacgct
    13921 tgcaagtgga ctacgataaa aacggtatta aaaaagcgaa caacgaaacg tttaaagaag
    13981 tttatgactt gtggctagaa acatataaaa ccactgtccg agaagccacc ttcatgaaaa
    14041 ctgaaatcaa gtttagaaag tggattcttc caaaatatgg aaatcttcgt gtgaatgaag
    14101 tcacggtaaa aaaagcccaa caaattgtaa atagctgggc tgaaactact gaccaataca
    14161 aggtacttca ttcaacttct aagcgtattt ttagctacgc tatcaatctt gggattattg
    14221 agactaatcc actagacaac gtattaatgc ctaaaagaac caccactaag cataaagaaa
    14281 aggtcaaagt ttacagtaaa gaacaactga tcaccttgtt tgattaccta aacagcaaac
    14341 ctgaaactta tcgaaacgac tttgataaaa cgttactacg ctttctcttt tatagcgggg
    14401 ttaggatcag tgaagccctt gcattgaatt ggtcagacat tgattttcaa gaaaatacgg
    14461 tcaccatcaa taaaacacta agccaatcca aaaatggcta taaaatcagt gatcctaaaa
    14521 ctgattgtag tgctggggtt cttccactag atgaaagaac aatttctata ttaaaaaaat
    14581 ggcagattaa tcagcggaaa tatatgctga cacttggtgt aactgatccg actatgattt
    14641 tttgtggtat ctataaagaa ctaatcacac accacgctat ttatgcaaga ttgattacta
    14701 tttgtgaaaa agctatggtt ccatttctcg gtgtgcatgt agcaagacac actcatgctt
    14761 caatgctctt ggacgctgga gctactatgg tagaagttca aactagatta cggcatacaa
    14821 aaattagtca gacgatcgat tgttacggtc acttggcaaa agaaacgaaa gaaaagactg
    14881 ttgaaaaatt agtacaacac ttaaatattg actagatggc atacaatatg gcatacagta
    14941 aaaaataaaa aaaggagaaa ccctatcata tcaaggtttc tcctagcttt atagtctatt
    15001 cttcttcttc cgccatgaat gtattgaata cttcttcaat catgtcccat tctgcgtctg
    15061 tttcgatttg ttctaactcg ccttctgttc catcttcgtt ttcgatgtat gcataagctt
    15121 gtaattctac atcttcatct tctgaaacac cagcaggata taaaagaaca tagttacgtc
    15181 caaattcttc ttgtccgtca actgttagta ggatttcata taatgtttca tttccttggt
    15241 catccaccaa tgtgatgtgt tcgtgtcctt catggtcgtg ggaatggtta tgttctgtca
    15301 taagattacc tcttttcttt tattattaaa ataattgctt acctgagccg tctaaataat
    15361 tttgtaagat catcacagct gctaatttat cgatgacttt ttttcgtttg gctcttgaag
    15421 tatttgcctg ctctactagc atacgttctg cctgaacagt ggttaatcgt tcatcttgat
    15481 aatcaactgg caaggagaat agttctttga tcttttctcc gtatgacatg gaagcttctg
    15541 cccttggtcc gatggaattg ttcatatttt taggtaatcc aactacaaac cggtctactt
    15601 catattgtgc tactaattcc ttcaaccgat caaagccaaa ctcttcttct gcttcattga
    15661 tccgaatgat ctctactcct tgagctgtcc agccaaaggg gtcactgata gcaacaccta
    15721 ctgtcttaga accgacatcc aagcccatta ctctcataaa ttcactccat gatttgagag
    15781 gtagtccttg acaattactt ccatgatctc atcgcgttcg tgacggcgaa tcaaatttcg
    15841 agcatcacga taacgaggaa tgtaggacgg gtctccagaa agcaagtagc cgacaatctg
    15901 attgattgga ttgtagcctt tttcttccaa agcccgataa acaatcgcta atgtttcgct
    15961 cacttctttt ttgcgactat cgtcaaaacc aaaacgtacg gtttcatctg taaaacccat
    16021 gattctacac ctctttcccg caaggtaatt ccagcttacc ttctacaacc ttattttact
    16081 cgaagaaagg gaaaactaca aacaaatatt ccaaacttcc gaataaatta agaaacacta
    16141 tttgtatgtt tttttccttt tttctgtagc tttatcaaaa gtactctttt cataaagtat
    16201 acgctatatt tctttgaatg tatgttttta cacaattttt tcataatatt cataaaaaat
    16261 tcgaatagct gctactaatt ttttgttttt gttgcttgtc ctgtctcata attgatcgtg
    16321 tagccatctt gaacattgta gataaagaca ttgaattcaa tttttttgtc ttcaatgctt
    16381 tctgcttcca attgcactcc cctggctacc agttcattcc cctcaaaatg aggagtgaca
    16441 cggtagcgta catgatgatt tgtttctttg atatatgccg caatatcatt ttcgtgagca
    16501 agcatgtatg gagcattcaa cgaacgtgtc cctgtcatca agtttttaat gttgttattt
    16561 tgaccagtca gctggaaacc aattaaatgg ctgcgattat ataaccattg cccgtcactt
    16621 aatttctttt gtttccaacc tgtcgggtca atatacaaag gctctctctt ttcagtaggg
    16681 aacaaatctt ttccaagcat agcattggct tgtcctactc tgttcaattt atcaatattt
    16741 gaatagcttt cccatgttcc tttggaaagg ctaagatcct cttttgtaaa ttccggctga
    16801 ttcccattga tttctatttc ttgatgtttt ccatcatatt ctggcacatc cgctacttga
    16861 gaagcttgtg gtgtggaatc aaaaatagtt gtaatctgct gaaagatatc tgaatctttt
    16921 tgtgtggtgg aaacaccgaa tgcccctgca atcacagcag cgacgatccc cacgatcgcc
    16981 ttggttgttt ttttgctcat ttctttactc ctttactaac agctccctaa atcttaacat
    17041 atgttcccct ataaataaat actagaaaga aatagaaaca gcaaaaaata aaacctgttt
    17101 taagatggct taaaaataca aaaaagagac ggattcctcg tctctaatca gtcaccactc
    17161 tatctgtaag gtatttatta ttattcaaaa attttcttca tactcaagac aatacactga
    17221 tttcacaacc gcttggtaaa cggtgc
//
LOCUS       DBNICI010000049        17136 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.049, whole genome shotgun sequence.
ACCESSION   DBNICI010000049 DBNICI010000000
VERSION     DBNICI010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.049"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(151..2058)
                     /locus_tag="AC1ZCC_001328"
     CDS             complement(151..2058)
                     /locus_tag="AC1ZCC_001328"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HIQ1629591.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            2188..3063
                     /locus_tag="AC1ZCC_001329"
     CDS             2188..3063
                     /locus_tag="AC1ZCC_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HIQ1629592.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            3143..4117
                     /locus_tag="AC1ZCC_001330"
     CDS             3143..4117
                     /locus_tag="AC1ZCC_001330"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIQ1629593.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            4297..5442
                     /locus_tag="AC1ZCC_001331"
     CDS             4297..5442
                     /locus_tag="AC1ZCC_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIQ1629594.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            5579..5923
                     /locus_tag="AC1ZCC_001332"
     CDS             5579..5923
                     /locus_tag="AC1ZCC_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HIQ1629595.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            6194..7480
                     /gene="efmA"
                     /locus_tag="AC1ZCC_001333"
     CDS             6194..7480
                     /gene="efmA"
                     /locus_tag="AC1ZCC_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HIQ1629596.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(7618..8217)
                     /locus_tag="AC1ZCC_001334"
     CDS             complement(7618..8217)
                     /locus_tag="AC1ZCC_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIQ1629597.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            8621..9742
                     /gene="mnmA"
                     /locus_tag="AC1ZCC_001335"
     CDS             8621..9742
                     /gene="mnmA"
                     /locus_tag="AC1ZCC_001335"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIQ1629598.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            9988..11409
                     /gene="arcD"
                     /locus_tag="AC1ZCC_001336"
     CDS             9988..11409
                     /gene="arcD"
                     /locus_tag="AC1ZCC_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HIQ1629599.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLITDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            11423..12541
                     /locus_tag="AC1ZCC_001337"
     CDS             11423..12541
                     /locus_tag="AC1ZCC_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HIQ1629600.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(12601..13893)
                     /locus_tag="AC1ZCC_001338"
     CDS             complement(12601..13893)
                     /locus_tag="AC1ZCC_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HIQ1629601.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            14043..14603
                     /locus_tag="AC1ZCC_001339"
     CDS             14043..14603
                     /locus_tag="AC1ZCC_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIQ1629602.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            14732..15109
                     /locus_tag="AC1ZCC_001340"
     CDS             14732..15109
                     /locus_tag="AC1ZCC_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HIQ1629603.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            15042..17114
                     /locus_tag="AC1ZCC_001341"
     CDS             15042..17114
                     /locus_tag="AC1ZCC_001341"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HIQ1629604.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         5473 a         3103 c         3651 g         4909 t
ORIGIN      
        1 gaaactaagc agctttttca caacctctaa tacatggtat tccagaagta acttcttcga
       61 aaaataagcc gaaatgtcac aaaaatttga gaagcgattt tcccaaattc cttcttattt
      121 cttgaagctg accacttctg gcacaacctc ttaccagatt cgcactcttt tttctggcgc
      181 tagatacata gcgtcttgtt ccgtgatacc aaatgtttca tagaattcgt caaggttttt
      241 tggttgcacg tttgctcgga gtttagcagg ggcatggaca tcgatttgca gtaaaagttg
      301 ttggtattct ttttttgctt ttgtacgcca aatcgttgcc caattgacaa agaattcctc
      361 gatagaaaga tctgattccg tttttgctgc ttccaatgca cagcttagac cgcctgcatc
      421 agcaatattt tccgaaacgg ttaattttcc gtttactttt ccgtcagcaa aaggcagacc
      481 atcgaattct tcgatcattg cttgtgcttt gttttgaaat tgtgtaagat cttcttctgt
      541 ccaccagttg ttcaagttgc catattcgtc aaataaagcg ccgttattat caaatgcatg
      601 ggaaatctca tgagcgataa cagctccgat tccaccataa ttagcagagc ttgattgttc
      661 tagactgtaa aatggtgctt gaaggatcgc tgccggaaag acgatcacat tccgaaatgg
      721 atgataataa gcattgaccg tgtcagcact catttcccat tctgaacgat ccactggttt
      781 gttccatttg ctgaaacgat cttttaatgt gagtttgctg aaaacaagag tatttgaaag
      841 caaagtacca ccctgatctg caggtactgt tttgaactgg tcgaataatg ctgggatctt
      901 atcggggtag cctacttgga tacctaagtt atttaatttt atgacggctt tttcttttgt
      961 tgcatcccca agccagtcat tttcttctag acgtttttta taaacggcga tcattttttt
     1021 caccatttgt tctacgtctt ttttagcagc ttcgccaaaa tatttttttc cgtagtaatc
     1081 gccaactact tgatcgaatt gtccagaggc aaggtaatac gctgcttttt tctgaggcat
     1141 tgcttcgtct gttccagaca atacgcgaga atagattccg ctgacttggc ggaattcttc
     1201 tgatagataa ccactaagag aacgtactgt tttaatgatc atccagcttt tgaataacgg
     1261 aaaatgagct tctgtcagga tgtcattcaa atgatcgaag tacactggat cagttacgat
     1321 cactttatct ggagttgtcc caatcaaacc cgtgacaagt tttttcaagt ccagtttatc
     1381 tgtgtattta ataaatgtat caaaagattg cggattatac attttgctgt aatctgcgtt
     1441 ttcttctgca ctttttacat gaggagcaat caatttatca aattgcactg cttgttccgt
     1501 gattgcttct gcctgacttt tttctttgcc ggcaagctct aataactgaa cagccatatc
     1561 aaagaaaacg cctattagtt gtgcaccgtt gggatggtct gatgcataat acgtcttatc
     1621 tggaaggaat aaagaaggcg gataagcaaa gaaggcgttc gtttgcgcat tcttcatatc
     1681 tgcatctaca tccagattaa atggcaatgg taatgtatcc aacagccatt ctggaaattg
     1741 cttattcaat tcgtcgaaag actgcaatga ttcaactttt tcgattatag gttgaagcgg
     1801 ttgagcatgc aactcatttc ttcggtcata atcgtttgcc agttgataat atgcaaggaa
     1861 atgtttcatt ttttctgaag acactttttc tggatgggca agcagatcgt ctgtttctcc
     1921 catcaaaagt tggtcgatcc cgtcaactaa atcttggaaa ccgcctgtag ctggtttatc
     1981 tgcagggatt tttgctgttt tttgccattc ttcattgact gcttcataga aatcttgttt
     2041 caatcgttct ttgttcattc atcgatcgct ccttttttgt ttcgtgtgcc cattatctta
     2101 caaaatgaag gaattaacaa atagaacata aaaaattgaa aattcgaaca atcaaactga
     2161 tttgtttttt tcaatccttc acttataatg aaaaaaagga ggcgatccca actgaatcaa
     2221 gtagtggata aacctttata tttcaaagta gataaaaaaa ttaaaaaatt agccagcact
     2281 cagcagctgc agtcaagaaa aagtaaattg ctttttttag ctctggtttt tgaagatcaa
     2341 agttatgtga ttatcgatca atcaggacat ccggtagaat attctccagc aaaatatact
     2401 tatcaagagg gtctttcttg taaaaaatgg aagctgatca atgaagagcc aatcgagtta
     2461 tctagatgga tcaacagaaa agaagatatt cccgttttga tcgaagaaaa aaggtcagga
     2521 aaggaactcg caaattgctg ggtgggattg ccagaagaac gttttttgag gtataagaaa
     2581 tgggctaccc cctcaggcta tctttgtggt acttacgcgg cagcagttct ccttgcctat
     2641 tatcaagact atcgtaaggg gtggatgctt ccaaatgaga ttcgcaagaa aaatacggct
     2701 gattcgttgg ttttgacgaa ggcattgcgt agccagatcc aaccactagg acttcctacg
     2761 attccttttc aggttagtac agggatttct agttttttaa agaagaatgg caatcatgag
     2821 cgtgcacgag caactcttct aggttcatgg cagcgagcta caaaacgtat acgtgaaggc
     2881 aaacctgtga tgattggtat tttgaaagta cttggcagta catatggcaa tcattgggtg
     2941 acagcgtacg cttattttga aactgaaaca ggagaacgtt attacaaagt acacgacaac
     3001 tggggcgatt atcataaagt cattcctgcc agttggagta acggaacagt ttcacttcct
     3061 taagaatttg ctgaaattaa cagaaaatct tggaaaaata tgttaaacta tcgaagaata
     3121 gagaagtttt ggggaggact acatgagtaa taagtatcag gatgacacgt tgaagatttt
     3181 tagtttgaat ggcaaccgtc cattagctga aaaaatcgcg aaagtgttcg gaacagaatt
     3241 aggtaaaagc gtcgtgaagc aatttagtga tggcgagatt tccatcaata tcgaagaaag
     3301 tattcgaggg gatcatgtgt atatcgttca atcaacgaat gcacctgtca atgattacta
     3361 tatggaacta ctgatcatga tcgatgcaat gaagcgagca agtgcgaaaa cgatcaatgt
     3421 cgtattgcct tactatgggt atgctaggca agaccgcacg gcgaaacctc atgaaccgat
     3481 cactgcgaaa ttgatcgcta acttgatcga agaagcaggg gcaactcgag tactgacttt
     3541 agatctgcac acggttcaag tacaaggatt ctttgatatt cctgtagata atttatttac
     3601 gatgccgttg tttgctcatt attaccgtca attaggtctg acgggagatg acatcgtagt
     3661 tgtttcgcca aaaaacagcg gtgttcagcg tgcacgtagt ctttcagaat atttgaacag
     3721 cacattagcc atcgtagatc atgcagatga tgcttcaact gagggtggtt atgtgatcgg
     3781 tgatgttcaa ggaaagacat gtatcatggt agatgatatc ttgaacacag gagcaacgct
     3841 tgcacgagca gcaaatgtat taaaagaaaa cggcgcaaaa gaagtatatg cctgtgcgtc
     3901 acatggatta ttgtccgatc atgcgaaaag tattttagac aatgcaccaa tcaaagatat
     3961 ttgtatcact gattctgttt tcacagaaga aaatcgtcat ccagaaaact taacaatcgt
     4021 tacatgttca gaattaatgg gtgaagcggt caaacgcatc catgaaaata caccgatgag
     4081 tccgttattc cgtctggaag aaaaagaatt cgaataaaca aaaaaaagag ggaaagaagt
     4141 tgtgacagaa gtgagcagct tcaaggagct gcgacaagac ttttgtcaca gctccttttt
     4201 tctatgccca tgaaacgtcc tctagacaaa aatgagctct tctttggttt gttcgctatt
     4261 ttatgctaga atgtgcctag caataatttg gaagggatga atgaagtgaa aactatttat
     4321 ctggatcatg cggcaactac ccccatgcat ccacaagtaa ttgaagcgat gacggcaatc
     4381 atgcaagaca cgtttggaaa tccttcaagc atacatggat ttggtcgcca cgcgcatgaa
     4441 aaattggaaa cagcaagaca aatcattgca gacagtctgc aagcaaaacc tcacgagatc
     4501 atctttaaca gtgggggaac agaaggggat aatacagcta tcttagaaac agcgttttcg
     4561 cgtcaaaaag aaggcagaca tctaatcact acagctatcg aacatcctgc tgttcttaat
     4621 accatgaaat atttagagac aaaggggttt gacgttacat atctgcctgt agatgaaaaa
     4681 gggaatctgt cgatcgaaga ggtcaaaaaa gcgttacgag aagatacgat cttggtctct
     4741 gtgatgtacg ggaacaatga aatcggaaat ttgatgccaa tcaaagaaat cggtgaactt
     4801 ttagcggatc atccagctta tttccataca gatgcggtac aagcgtacgg aaaccaaacg
     4861 atccatccag aagaattagg aatcgattta ttaagtatct ctgctcataa aatcaatggg
     4921 ccaaaaggcg tcggtttctt atataaacga gacggagtaa atatccctgc attgctttta
     4981 ggcggggagc aggaagaaaa gcggcgtgca ggtacagaaa atttagcagg gatctggggt
     5041 atggcaaaag cagttgaact gctgtcacct gaagaacgtg aagcacgtaa caacaaatat
     5101 aaaggattcc agcaattgat tcttgataca ttagaagcaa acgaaatccc ttatcaagta
     5161 aatggtgatt taacgaataa actccctcat gtgttgaatc ttcgcttaat tggcgaagca
     5221 aatgatttgc tactgatgaa gcttgatctt aatggggtcg ctatttctac tggttcagct
     5281 tgtacagcag gaaatatcga gccttcacac gtattagaag caatgtatgg agaagataca
     5341 ccgatactaa aagaatccat ccgtatcagc tttggtttgg gaaataccga agacgacatc
     5401 cagtatttcc ttgatcaatt agttcgtgct gtcaaaaaat aactagtgaa gtcattctta
     5461 tatgaacaga tgtctgagag aacgccttca ttttttaggg aacttaactg aaaaacctag
     5521 taaaaatcag gataaacttt tataataaag agaaactaat tttatcgagg tgaaaatcat
     5581 ggcatttgaa acaacagcaa cggtactggg cgcaccaatt agctatcgtt tgcatcctga
     5641 tgcaaaaaga tatacattgc gcgataatgg atttacagaa acaaacggag gcaattatca
     5701 attgatccga ccattagatg ccacgccgca aagcaaagaa ggattcaaat taaaaatcac
     5761 tgtatcaaaa gatattcaaa cattgaaaat gtcgatcact acagcaaatg gattgaaagc
     5821 agtcaatatt ttcaaagatc caaaacagaa aatgagccaa gataaatttt attttctgat
     5881 ggatgggatg attagccgtg ggttattcga aaaagtcgaa tgatctgtgg atataaaaag
     5941 acaactagtt ttgaaacggc ctattgttag atgactaact ttgtaaggac gacgcaaatt
     6001 agttgtcttt tttattttta aatttaacac atgttgttat ttttaatatt ttgctgattt
     6061 attgtttcta agtaaagaaa tttaaattga aacgattgtt atttagggaa aaaggtattt
     6121 tttgaaaaaa gaatcccgta taatgattgt tgtgcaaacg ctatacaaaa tgaaaaagaa
     6181 aagagggatg tttatggaaa atgaacagtc ggtcgtttta acgaattgga agagaaatta
     6241 tttgttcttt ttatcgggac agtttttgtc aggaatcact agtatggtcg tccaatatgc
     6301 gatcatctgg tatttgacaa gagaaaccgg ttcggcgaca attttgagtt ttgctacttt
     6361 actaggaatg attcccatgg ttttactaag cccatttgta ggaccgctag tggatcgctg
     6421 ggataaaaaa gctcttttga ttgtcacgga tattattgta gcgattttcg cgcttatttt
     6481 agcagtagtc ggaacgattt cagaatcctt cccgatttgg ctagtttttg tgtctttgtt
     6541 tatgcgttcg gtcgcacaaa cattccaaat gccaacgatc cagtcgatta tgccgacaat
     6601 cgttccatct tctcacataa caaggacgaa tggacagtta ggaatggtcc aatcagcgaa
     6661 tttcatcatt gcgccagctc taggtgcggc tttgttttca gtcgttcctg tcaattacct
     6721 tattttatta gatgtattgg gggcagtttt tggtgtcggc ttattgattt ttgtgaagat
     6781 cccaaaagtt tctcctgaaa tattggaagt tccgcttact atttttaaag atgcaaaatt
     6841 tgggctgcag caattgatgg ataataaagg gttatggtac ataacgatca atggtgcatt
     6901 tgtgatgctg ttgttcatgc cagcaatcag tctgtatcca ttgatgacgc tagattactt
     6961 tggtggttcc gttggacagg cgggagcagt ggaagtagtc tacgcagtcg gaatgctctt
     7021 aggcggtgca ctgatcagtt ttataggaac atggaaagac cgaatgaaac ctattattat
     7081 agcctacatc attatgggcc tgacgatcgg tgcaagcgga ttggttccaa acgatagtca
     7141 agggttcttg tactttctta tcttgaatgc aggagcaggt tgtgcaacgc catatttcaa
     7201 tacactgctg atggcaatga tccagcagag ttatgaatcg aatgttttag gtcgtgtctt
     7261 agggaacttc aactctttga tgaacctggc aggtccaatt ggattattat ttgcaggacc
     7321 attagctgat cgtttagggg tagaaaaaat gttcttgttt tccggaatcg ggattttatt
     7381 atgcggaatc gttttatttt taacgtcagc tgcccgaaat tatgataaag aattacagaa
     7441 aaaactggta aaagaacacc atgagcaaaa agacgaatag tatggaatgg agttgtgaca
     7501 gaagtcttgt tacaactcca tttctgatcc taatgttcct gtggcaataa gcctactcaa
     7561 atgaaaaata tgagtaatta tctttatttg tttaatactt gatatagggc agttttttca
     7621 cgacctatcg tacttcctct ttgcttcata gatttctttg atttgttgct cagataacgg
     7681 agtgccaaat ggtagttttt cctttaagag ttctctttcc ggtgcgtcta cgcctacatt
     7741 gacatcgttc tcgataggta cgctgtaatg atggatatgt ccatgaagat tgcggatagt
     7801 gtcgttaggc ccgagcaaca tcggataatg agtcaagtaa tattgttgat ggtcgaattt
     7861 aacgatggca cctacatcat aaaaataaaa tttgggactt ccgtcagtaa gcctaggatc
     7921 atttttttct aaataacgga aaaaagcgcg actgtcatgg ttccctttga tcaagtggat
     7981 tgttccttgc agttgagtga ccagttccaa aatttctgca tttgcttttt catattgcgg
     8041 atgcatagca aggtcgccta gatgataaac atggtcggtt tcttttatta ctgcattcca
     8101 gctagtgatc aactgttggt tcatttcatc cacatttgca aagagccgcg gagcaaagtc
     8161 gttttttcct aataagcgtt catggaaaaa gtgcatatca ctgataaagt aattcatgta
     8221 tcttttgcct cccttttttc ttagagtaaa caaaatcact tatctgagca actcataaca
     8281 tctaaaaaga aaaggttgta aatgagacgt gcagctttaa gcgataagaa gcaattttga
     8341 aaaatagttt ttcacaattt ataaaattat tccttattgc cgaaaggctg gcccttttta
     8401 tgagtatgcc aattttttat atcgaatgct caaaagatga gtgatttttt ggttgactat
     8461 catgtttgtg tactgaatag gtccagtaga acagtatttt tacgacttga taattaaggg
     8521 atgaaatttt cagaaatatg acgtataatg taaaatactg aaaagttgcg accttcaact
     8581 cgtagatgtt tcagaatcta attgaaatga tggtgatatc atgacagaca acagcaaaac
     8641 acgcgttgtc gtagggatga gcggcggtgt cgactcatct gtaacagcac ttttgttaaa
     8701 ggaacaagga tacgacgtga tcggaatatt catgaaaaat tgggatgaca cggacgaaaa
     8761 cggtgtatgc acggccacgg aagattataa ggatgtagcg aaagtagctg ctcaaatcgg
     8821 gattccttat tactctgtga attttgaaaa agaatattgg gatcgtgtat ttgaatattt
     8881 cctagccgaa taccgcgcag gacgtacacc taacccagac gtgatgtgca acaaagaaat
     8941 caaattcaaa gcattcttgg attatgcaat ggatcttggc gccgattatg tagctactgg
     9001 acattatgcg caagtcactc gcgatgaaaa cggcgtggtc cacatgcttc gtggagtaga
     9061 caataataaa gatcaaactt acttccttag ccaattatca caagaacagc tttcaaaaac
     9121 gatgttccct ctaggcggca tggaaaaatc agaagtacgg gcaattgctg aacgcgcagg
     9181 ccttgcgact gctaagaaaa aagattcaac gggaatctgc tttattggtg aaaagaactt
     9241 caaacaattt ttgagcaact atctgccagc aaaaaaagga aatatggtca ctcttgatgg
     9301 tgaagtcaaa ggacaacatg ctggcttgat gtattatacg attggtcaac gtcaaggact
     9361 agggatcggt ggcggaggag attcgcaaga accctggttt gttgtaggaa aagatctagc
     9421 gacaaacaca ttatatgtgg ggcaagggtt ccatcatcct gcattgtatg ccacaagctt
     9481 ggatgcaagt gaaatccact ttacgacgaa tgaaccaatg ccaaaagaat tcaagtgtac
     9541 agcgaaattc cgttatcgtc agcaagacgt tcctgtgaca gttcgcttat tggatgataa
     9601 tcgtgcagaa gtcgtattcg acgaacctgt acgtgcaatc actcctggac aagcagttgt
     9661 attttatgat ggaatggaat gtctgggtgg tggtttgatc gaccacgcat atcaagaaac
     9721 aaaagtttta caatacgttt aagcgatatt aaatgaatca aaacagctat ggctaacaaa
     9781 agaaacacct tttcttttgg atagcatagc tgtttttttg caataaaatt aatttttatt
     9841 cattttatac ttgaaatact tgtgtaaaaa gttcataata tccaataata tgaataaagt
     9901 tgctttttgt taagcaagag cagttgcaaa atagaaggga atcttttgca agtgatttgt
     9961 tcaattttat aagtgagggt gatggaaatg gaaaagaaat cgacgggtat tagcaaatgg
    10021 ggactggttg ctctggttgt cagttcgtca atcggaagcg gtgtgtttgg gatcacaagt
    10081 gatctggcta gttctgcagc tccagggcca gcgatccttt catggataat cgttgggatt
    10141 ggtattttag ctttggtatt atcgctgaat catttaggag aaaagcgtcc agatctagat
    10201 ggtggtattt tcggttacgc ggaagcttca tttggtaaat taggtggctt catcagtggt
    10261 tggggttact ggttgtctgc gtggctggga aatgttgctt ttgcaacgat gctgatgagc
    10321 gccatgggag aatttttccc gatattcaaa ggtggacaaa atgttccttc tattttattt
    10381 gcaagtattt ttatttgggg tctgacttta ttagtaaaca atggagttga aggagcgagc
    10441 gtcatcaata caatcgtgac gatctgtaaa ttagtgccat tattcctatt tttaatattt
    10501 gtatttatcg catttaaagt gaatatattc actgctgatt tttggggtaa cgtatctgat
    10561 aatctagtga atggaacagg gcagcaagga acgatctggt tgcagattaa aggttgtttg
    10621 atggttatga tgtgggtctt tgtagggatc gaaggcgcaa gtgttttagc caaccgcgcg
    10681 aagaaacgtt cagaagccca gcaagcatcg atcattggtc tgctttgttt attgtttatc
    10741 tatatccttg cttcgatctt gccatacggc gtcttatctc aagaagagtt agcaactatt
    10801 tcacaacctg ctatggcaaa tattttaaaa ggaatcgtgg gaaattgggg agcagcgctg
    10861 atcaatatcg gcttgattat ttcgatcatt ggttcttggt tgtcttggac gatgctccca
    10921 gcagaaacaa cgatgttgat ggctcgagac ggtctgttgc cgaaaggttg gggaaaaacg
    10981 aataaaaaaa atgccccaac ttattcgtta gttgttactg caatcttgac taatctattc
    11041 ttattgactt tcttaattac tgattatgcc tatcaatttg cttattcttt atgtacagcg
    11101 gcaattttga tttgttatct acttgtcggg atctatcaag tccagttttc tttaaaagaa
    11161 ggaatcaaaa aacaagtagt gatcggtggg attgctgtag tttttgaatt gatgggcatc
    11221 attttagcag gattttctta tgtcctttta tgtagtattg cctatcttcc tggtttttgc
    11281 ttctactgga tcgcttgtcg tgaaaccaaa catcaaatta cgggaaaaga aaaagtaatg
    11341 attgggctga tttgtgtggc cgcactgctt tcaatcgggt tgcttgctgt aggctggatc
    11401 aagatataag ggggaaagaa acatgaaaat cgcaaatttc ggtgtagaag aatggctaaa
    11461 taaatgggaa aagaaagcgg tatatgatat tgcacaaagc tcgatcgaag cattgacatt
    11521 agacgaactc gttggcttag atgggactag cgtaagtaaa tttttcgaaa aacgcaaaaa
    11581 cgaaccattg gactatggct ggatcgaagg atcagatgtt ttcaaacaag aagtcgcttc
    11641 tctttatcaa accgtcgatc cggaaaacat cttacaaacg aatggagcga ctggtgctaa
    11701 tcttttagct ctatacgcgt tagtggaagc aggagaccat gttgtttcga tgcttcctac
    11761 ttatcagcaa ttatatgaca tccccaaatc tttaggtgcc gctgttgact ttgttcattt
    11821 gaaggaagaa gaagattggc agtttgattt agaacagcta gaagccttag tcaaacctga
    11881 aacaaaaatc atctgtttga atagtgcaaa caatcctaca ggcacattgc tagatcggaa
    11941 aactttagaa aaaatcgcag aaattgctcg tacagtagat gcatatgtat tgatagatga
    12001 agtatatgcg cctttaacgg ataaaggtga attcttgtcg atcgtagatg tctatgaaaa
    12061 aggtatagca accaattccc tatccaaaac ctactccatt ccaggtattc gtattggctg
    12121 gacagctact ggacctgagt tggcagaagt ttttcgaaag tatcgagact acacgatgat
    12181 ttgcggcgga gtcctgtcag atgatcttgc tgtacatgca ttgaagaata aagaaaaaat
    12241 attggcgcga aacaaaaaga tcattacaga gaaccttgtg attttaaaac aatgggtagc
    12301 caatgaaccg aaagttgaac ttgtcgctcc caattacgtc tctacttctt tcattaaatt
    12361 agccatcgaa gaagatgacc agacattttg tatcaattta ttagaagaaa caggagtact
    12421 attagtacca ggaagtgcct ttgatttacc gaagcatgcc cgactaggtt actgttgtaa
    12481 aaaagaaacc ttggaaaagg gattattcct cttatctgag ttcttgaaaa aacaggccta
    12541 aacaaaaaca ctgcaaggac agtctgtgac aactgttcaa gcagtgtttt tcatttacgc
    12601 ttattgtcta tgagaaggag agtgccctga agctatggag actcctgaag ctttttcttc
    12661 tgcagtttct tgctgatgga tctcttgtgt attgtgtcgt acaagcaaga agttgaccaa
    12721 aacaagaaga gaagtcgaaa gaaagacgcc gcgataacca aatgcactgg aaacactgga
    12781 gccgatcata ggccctacta cgttaccagt tgcttggaaa gattggttat aactgaagat
    12841 ccgtccagca gcttgatgag gcgaatattt tgtgatcaat gcttggacag cagggagcag
    12901 acaagcatca gagataccaa tcaaaaaacg taaggcagct aattgccaaa cattcgtgac
    12961 gaatgccatt gggatataaa ccagaatcgc aaaacccaaa ccaatcgcaa gtattctttc
    13021 actcccaatc ttgtcaccta agcgaccaaa tcttggagcg gctatcaaag tggcaatccc
    13081 aggtatcgaa gcaatgattc cgctgatcaa cgtaacattc ccatgtccat gcagcaattg
    13141 gcgaatgtac agactgatga ttggactaat ggaattgttg gatgcttgga tgatcatcgt
    13201 tgtgacgaac ataccgatga cgacgtgtgg atatttcaag tctttgaaaa tctgtttggc
    13261 agaaaccatg ttttcttttt tcactgggac aaatttttca tggacgaaaa acagactcaa
    13321 caagaagacg aaaaataaga tgatccctgt aatgaaaaag gtagggcgat agccaaacgc
    13381 tgaagcagag atcccgccaa gaagcggtcc aagtaatgtt cctgtaacag aaccagttgc
    13441 taaggttcca agaacttgac cacttttttc ttttggcgtc ccagttgcga caagggcagt
    13501 ggcattactg atataaccag aaaaaatccc ttgaagcaga cgcaatgcga ctaattgata
    13561 cacactagtc acgcaaccca tcaaagaaat gacgattgcc attcctaatg aagcacgcaa
    13621 cagcatcaat tttcttcctt tttggtcggc taattttccc caccaaggag agataagcgt
    13681 agtcaccaag aaagtagatg aaaaagtcaa tccgctccaa aagttcaatt gtgacgtact
    13741 ataattacct aacgtatcta tgtaaagtga cataaaaggc atgactaaac taaatccgat
    13801 accagccata aatgttccaa accataagac aaacaagttt tgttcccaag tttctctttt
    13861 cctaaataat ttttccttta ttcttgcagc caattagact ccttctttct gaaaattttc
    13921 atctacagct ctttattata aactacaata tgtagtttat caatgaaagt ctcttttttc
    13981 tctttctttt tcgtggtaaa ctgaaagagt gaaggattta ttttatgaga ggaaacatgg
    14041 aaatgaaaaa aagaaatctg tctcaagaga aaatcatcga ctgctttcga gaactagcag
    14101 aagagatgga tgttcagcaa ataacctttc agcatttagc caaacgcttg gatatcaagg
    14161 ctccctcgct gtataaccat ttcaaaaata ttcgtgatgt caaaacagcg ctaacagcga
    14221 aacttctgca ggaacttaat gaccagctgc gacgggctct tgtgggaaaa agtggtcagg
    14281 aagcactgca gatctatgca gaaacttatc gatcctttgc tttttcaaat caagcagtgt
    14341 atgagttact gatcagtgta ccgcatacca atgaagctgt tttattagat ggaatccatg
    14401 aaacgaatca gatcattttg cagatatttg aggcttttca attaacgaaa gaagaaaaag
    14461 ttcatcgaag cagggaactt cgtagtatga tccacggtta tctttcctta cgttttttag
    14521 gatactttac aaaagaacca acaatcgatc cagaagacag ctatttttgg atgatcaata
    14581 actttatcgc tactttgcct taaacaatca gattcatttg taacaatttt aagatgttca
    14641 ttaaatgacc cttttataag agaaaattct tgcaactttc ttgaaaagtg actaagatga
    14701 gtctagaaaa gctgaaagaa ggagcacgag tatgtatcaa gtaattacca tgttcgggga
    14761 caatgagcct tggtggtttt ttgaagactg ggaagaagat attgaagaag aagaaacatt
    14821 cgagtctttc gaagaagcac gacaagcgta cgaaaaacaa tggaacgaaa tccatcaaaa
    14881 ttatgaatat atcaatgcaa aaagtaactt tctcagcgcc ttttggaatg acggagatga
    14941 gcgctggtgt gaagagtgtg acgatgattt acagcaatac aagggacttg cgttgttgaa
    15001 aaatcatcag cccatcacag tagaaagcag aaaggaattt tatgaaacaa ctaattctag
    15061 cggaaaaacc aagcgttgcg aaagacctaa gcaaggtgct tggtgctaat caaaagcaca
    15121 aaaattatta tgaaggaccc aaggtgatcg ttacttgggc ccttggtcat ttattaggat
    15181 tgaaaatgcc ggaagatctg aataaggaat ggcagagctg gcaaatggac acgttgccaa
    15241 tgatccctaa aaaactagga atcaaaccac ttcctaaaac aggacatcaa ttaaaagcaa
    15301 tcaagcagct ggctcagcga aaagatgtct cagaagcagt cattgcgaca gatgccggaa
    15361 gagaagggga acttgttgct cgctggatct tagagtatgt gcatttcaat aaaccagtga
    15421 agcggttatg gatctcttct caaacagata aagcaatcaa aactggattc aagcaactaa
    15481 gacctgcgaa agaatatgat gcattgtatg aatctgctct tgcccgggca aaagcggact
    15541 ggctggtagg attgaatgtg accagagcct taactgtgaa gtacgcggat aatttatcag
    15601 caggccgtgt gcagacaccg acactagcat tagtgagaga ccaagaaaag aaaatcgagc
    15661 agttccgtcc acaaacgtat ttcacgatca cgttgcaagc tggaaaagaa caagccaaaa
    15721 tgcttcaaaa aaatcaattt gctttaaaaa cacaagaaga agcacaaaaa cttgttcaat
    15781 tgatcagtac aagcaaagga actgtcacag aagtccaaga aaaattaaaa acggaatctg
    15841 cgccattgcc ttatgattta acagagatcc aaagagaagc caatcaacgc tatggctatt
    15901 ctgcaaagaa aacattaagt cttgtacaaa gtttgtatga gacccataag atcgttactt
    15961 atccgcgtac agacagcaaa tatctgacaa atgacatgaa aagtacgatg aaagaacgat
    16021 tgcaagcagt cagcgacttt gctcctgagg tcaagcagta tttgaaaaac ggagccatcg
    16081 tccaacagac aaaagtattc caagacagta aagtgaccga tcaccatgca cttttgccaa
    16141 ctgaaaacag agcacgttat gaaaaattaa gtaatgaaga gcgaaaaatc tatcaaatga
    16201 ttgtctctcg ttttttagga ctgtttgctc aaccccataa agtcagccaa acaaaagtta
    16261 cggttgaatt tggtaaagaa cgattcattt ttcggcaaaa tcgtgtcgtc caagcaggat
    16321 ggaaaggcga aactgaaact gagacggaaa cagtcaaatg ggaaaaagga atgagaatca
    16381 atcccgattt cgccatcaaa aaagaactat ctgcaccgcc taaaccattg tcagaagcaa
    16441 gcttgcttgg tcaaatggaa aaatacagtc tgggaacccc agctacgcgt gcagagatta
    16501 tcgagaaact aatcaaatct gaactgatgg aacgtacccc tcaaggactg caagtatctc
    16561 ctaaaggtaa gcaattatta gtattggtaa atccctcttt agtaacacca gatttgacag
    16621 aaagctggga aaaagacctt gaagctattg ctgcaggaaa gaaacaagca ggaaccttct
    16681 tgaatggaat cgaaaaagaa acaaaacgct tggtgaatga gatcaaatca agcaaacaag
    16741 aatatcaaga tttctcgatt acccaaaaga aatgcccaga atgtggggcc aatctgcgcg
    16801 agaaaaacac acgtgacggc aaaatctatg tctgcaccaa ttcagattgc aactaccgca
    16861 gaagaaaaga tccaaaagtc tctaatcatc gatgcccgca atgtcaccgg aaaatggaaa
    16921 tcatcgaagg taaaaacggt gcttatttcc gttgtaaata cgatggaacg acagaaaaat
    16981 tgcttgataa aaaagagcag aagaagaaaa tgaccaaaca tgaagaacga cgcttgctga
    17041 aaaaatatgc acaagcagaa gaaccagaag aaagcccatt agcagcagca ctaaaagcag
    17101 caatgaagaa gtaataagat atgaaagagg ttgtga
//
LOCUS       DBNICI010000050        16962 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.050, whole genome shotgun sequence.
ACCESSION   DBNICI010000050 DBNICI010000000
VERSION     DBNICI010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.050"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(58..579)
                     /locus_tag="AC1ZCC_001342"
     CDS             complement(58..579)
                     /locus_tag="AC1ZCC_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIQ1629605.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            777..4457
                     /gene="nifJ"
                     /locus_tag="AC1ZCC_001343"
     CDS             777..4457
                     /gene="nifJ"
                     /locus_tag="AC1ZCC_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HIQ1629606.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            4646..5533
                     /gene="cdaA"
                     /locus_tag="AC1ZCC_001344"
     CDS             4646..5533
                     /gene="cdaA"
                     /locus_tag="AC1ZCC_001344"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HIQ1629607.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5530..6699
                     /locus_tag="AC1ZCC_001345"
     CDS             5530..6699
                     /locus_tag="AC1ZCC_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HIQ1629608.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6723..8078
                     /gene="glmM"
                     /locus_tag="AC1ZCC_001346"
     CDS             6723..8078
                     /gene="glmM"
                     /locus_tag="AC1ZCC_001346"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIQ1629609.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8150..9955)
                     /gene="glmS"
                     /locus_tag="AC1ZCC_001347"
     CDS             complement(8150..9955)
                     /gene="glmS"
                     /locus_tag="AC1ZCC_001347"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIQ1629610.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            10365..10655
                     /locus_tag="AC1ZCC_001348"
     CDS             10365..10655
                     /locus_tag="AC1ZCC_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629611.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            10668..11693
                     /gene="sppA"
                     /locus_tag="AC1ZCC_001349"
     CDS             10668..11693
                     /gene="sppA"
                     /locus_tag="AC1ZCC_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HIQ1629612.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            11696..12355
                     /locus_tag="AC1ZCC_001350"
     CDS             11696..12355
                     /locus_tag="AC1ZCC_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIQ1629613.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            12512..13444
                     /locus_tag="AC1ZCC_001351"
     CDS             12512..13444
                     /locus_tag="AC1ZCC_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629614.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            13598..14887
                     /gene="tig"
                     /locus_tag="AC1ZCC_001352"
     CDS             13598..14887
                     /gene="tig"
                     /locus_tag="AC1ZCC_001352"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIQ1629615.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            15134..16384
                     /gene="clpX"
                     /locus_tag="AC1ZCC_001353"
     CDS             15134..16384
                     /gene="clpX"
                     /locus_tag="AC1ZCC_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIQ1629616.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            16467..>16962
                     /gene="yihA"
                     /locus_tag="AC1ZCC_001354"
     CDS             16467..>16962
                     /gene="yihA"
                     /locus_tag="AC1ZCC_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HIQ1629617.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDF"
BASE COUNT         5544 a         3025 c         3723 g         4670 t
ORIGIN      
        1 gtgtttagag cgtgagacaa aagtgttctt tacttttgtc ctacgctcct tattttttta
       61 ttttgtttgg atcaattctt ttaattcctc tattgaaata gattcatcaa agatttcttt
      121 acccaagatg acagtcggaa caaatcgaat atgtgcttgc tcagcttcgt tgatgacagc
      181 ttggctcagc tgttcgtctt tttgttctgt cagctttagt ttatctactg caaaatccgc
      241 tacttcttgg agagaaagat gtttccactc gtcttgagta tcgaatattt gtttgatctc
      301 tttgatcgct ttttgaccat cgctgattgt gagataccgg tgcatcacgt ttcctcgctg
      361 caagctttct ttttctttat cgaacaattt gatcactcgc tgcaattgcc cgctttggac
      421 tgcttcttct aatagctcat aagattcttc aaaccattgt cggcaatacg gacaagatag
      481 gttgatgaat tcgaccattt ttttaggagc attcgatgaa ccataaagga ttcctttttg
      541 tgtatttgtt ttcgttgcat caatcactga tatatccatg ttaaaacacc tctttcgctt
      601 atagtttata catgtctgac aaaaaaaggc aacgatttag actgtttgtc taaaaattag
      661 atgaccaatt caaatagaca tatgtgaaat atttttataa ataaaagaaa aaatggaatg
      721 ttttacgtta taatggaaca gataataaga gagaatatga acaaggatga tgaatcatgc
      781 gaaaaatgaa aacaatggat ggcaatacgg cagccgcata tatttcttat gcatttaccg
      841 agattgctgc aatatatcca ataacaccca gttcgaccat ggctgaatta gtagatcaat
      901 ggtcagcagc aggaaaagaa aatgtattcg gtcaaccagt aaaagtagta gaaatgcaat
      961 ccgaagcagg agcagctggt gtggtccatg gatcattgaa aactggtgca ctgacaacta
     1021 catataccgc ttctcaaggg ctgcttttga tgataccgaa tatgtacaag attgctgggg
     1081 aactactgcc aagtgttttc catgttgcta gtcgggcagt gacgacaaat gcgttgaata
     1141 ttttcggaga tcacggagac gtgatggctg caagacaaac aggatttgct atgttagctg
     1201 aaagcagtgt acaagaagtg atggatttag cacctgttgc ccatcttgct tctattgaag
     1261 gagaagtacc ttttttgaat ttctttgatg gattccgtac gagtcacgag attcaaaaaa
     1321 tcgaagtttt ggaatatgaa gaattagctc ctctgatcaa tcaggagaaa ttagcttcat
     1381 ttcgtaaacg agggatgaat cccaatcatc cgactgttag tggtacgaac cagaatccag
     1441 acatccattt tcaacaacgg gaaacgatca acccctatta tgataaatta cctgaaatcg
     1501 ttcaaaaata tatgaaagag atcaaccgat tacgaggaac ttcttatgac ttggtgactt
     1561 attacggagc agacgatgcg gaagaagtca ttgtttctat gggatcagtt gcacagacga
     1621 ttgaacaaac gattgattac ttgaatgcaa acggacggaa aacaggtttt ttgaatatcc
     1681 atctttatcg tccttttcca gtggaaacat tcgtgcaaaa gttgcctcat acagtaaaaa
     1741 caattgctgt tcttgatcga agtaaagaac caggggcagg tggagaacct ctcttattag
     1801 acgttcaaag tgcgttatat gattctgaac ttcgcccggc tgttattggt ggtcgctacg
     1861 gattggggtc taaagatgtg actcccgacc aaatctcagc agtttttgat gaattgaaaa
     1921 aagacccaag catacgcaaa aaacggttta ccatagggat cgtggatgat gtgacttatc
     1981 aatctttgga accaaaggaa agtctggatt tgacagaacc gcaaacattc caggccaagt
     2041 tttggggatt tggatctgac ggaacagttg gagcaaataa atcagctatc aagattattg
     2101 gagatcacac cgataaatat gcgcaaggct atttttatta tgattcgaaa aaatctggcg
     2161 gtctgactgt ctctcatctt cgttttggag atacgccgat acgttcagcg tatcttgtcg
     2221 agcatgcgga ccttgttgcc tgtcacacac cagcttacct gcatagctat gacttggtca
     2281 aaggattgaa gccaggtggc atttttttat tgaatactct ttggagcgac gaacaattag
     2341 aaacgcatct gcccttaaaa ttaaaacgtt acttggctga aaataacatt agattctata
     2401 cgatcaatgc catgagattg gcccaagaag tcggtttggg acgccggatc aatacggcaa
     2461 tggaaacggc tttttttaaa ttagctgaca tcattccttt tgatgaggtg cttccgctgc
     2521 taaaagaaga agccctgaaa agctatggac ataaatcaat ggctatcgta gaaaaaaaca
     2581 tacaagcaat cgatcggacg accgaactgc ttcatcaagt cgcgatccca gaccattgga
     2641 gaacgttgga gatgcctgaa aaaatcaata aaaggaacac tggttatata caagaaatac
     2701 ttgaaccagt gaatgcacag gaaggaaatc aactctctgt tggtacactt gtagcaaatg
     2761 gaatgacaac tggcgaaatg cctttaggaa cggcttctat ggaaaagcgg ggcgtcgctt
     2821 tagaagtacc tgagtggatt agtgatcgct gtacgatgtg taatgaatgt gcctttgttt
     2881 gtcctcatgc tgccatccgt ccatttttag cagatgaaga ggaaatggaa gaagcgccag
     2941 aaggatttat cgtgagagaa atgcgaggcg cagacggcgt aaaataccgt atccaagttt
     3001 cagtggaaga ctgtacaggt tgtggtctgt gtgtagaggc ttgtcccgca aaagaaaaag
     3061 ctttagtgat gaagccttat gaagaacaga aagaacaagc tgtgaattgg gcttttgcaa
     3121 tgacattaag acaaaaagca aatccagtca aaggaaagaa tactgtcttg tctacacaat
     3181 tcaatcaacc gcttcttgaa ttctcgggcg cttgctcagg ctgtggcgaa acaccttatg
     3241 taaaattatt gacacaaatg ttcggtgatc gaatgatgat cgcgaatgcg acaggctgtt
     3301 cttctatttg gggcggtgct tctcctgctt ctccttatac gaccaatgaa tgtggtcaag
     3361 ggcctgcatg gtcaaattct ttattggaag ataatgctga atttggttat ggtatgcttc
     3421 tagcggcaca gactcgtcgc gaggctttgg caagttcaat caaagaagta ttgccaaaag
     3481 tatcagaaga actacgagaa ttgatggaag actggttggt acacatgttt gagagcgaag
     3541 gaacgcagca aagagcagca aaactgcaag ctgcgatcga aagcgaacga tcaattgtcc
     3601 ctgaactgga ggatatatac aagagaaagg atctattcgt aaaaaacagc caatggatga
     3661 ttggtggaga cggatgggct tacgacattg gttttggcgg gatcgatcat gtacttgcca
     3721 gcggagcaga cgtgaatatt cttgttttgg ataacgaagt ctattccaat actggcggtc
     3781 aaacctcaaa agcaacacca gcttcggcaa tcgctaaatt ttcggctagt ggaaagtatg
     3841 cttcaaagaa agatctagga atgatggcga tgacatatgg aaatgtttat gtggcgcaaa
     3901 ttgcttcagg tgccaatcaa atgcagacga taaaggcttt tgaagaagca gaaaagtatc
     3961 ctggaccttc tttaatcatt gcctatacgc cttgtatcac acatggtttg cttggtggaa
     4021 tgaaagaatc gcttaaagaa gcaaaagaag ctgtttcttc tggctattgg tcattatatc
     4081 gatataaccc tgcaatggtc gaaaaaaaca agaacccaat gactttggac tacaaaaaac
     4141 ctgatttctc tgctatgcct gatttcatga ggaaacaagt tcgcttttcc tcgctagaga
     4201 acgcaaatcc aaatcttgca aaagctttat ttgaaaagac tatccaagac gctaaaacac
     4261 gtttttacaa ctatgctgca ctgacgggag aattagaaaa aatcaagaaa cgacttgaac
     4321 cagaggaatc tatcgatgta cccgttaaga ctagaaaaga aaaaacgacg gaccttgaag
     4381 cagaagaaag aagagctgcc agagcagaac gacgtaaaaa aatggaacaa gctacaaaag
     4441 aaaaaaagga aaaataacag aaaaaatata atgaataatt ctctgaagtt taaaaatatt
     4501 tattgcagag aaaaataaaa cgcatacttg caattttcct gaaaattatg cgtttttctc
     4561 aagaaaaata cgagcagatc agcctctaaa attaaaacat atgatatact agagaggaaa
     4621 tctcaaaaga gaagagaggc tttttatgtc atttcaaatc ggggaacttt ttaatttgga
     4681 ctattggcag caaatgtttt ccggtgactt gttctctctg aattttttgg tgaacatact
     4741 tgatattctg gttgtctggt atcttgtata caaactgatc caactgctgc gaggcacaaa
     4801 agcgattcag cttctgaaag gtgtagctat ttttattgtt atccgaatcg tagcggaaat
     4861 cattggtctg cacaccttgt cttggctgat gaaccaagtc attacgtacg gggtgatagc
     4921 tgctatcgtg atttttcagc cggaaatccg gagaggactg gaacatcttg gacgcagttc
     4981 gcttttcaaa caatcaaaaa gcgaacagca ggaagatgag aagatgatct tgtcttttga
     5041 caaagccatc caatatatgt ccaagcgaaa aatcggtgcg ctaattacta tcgaacggca
     5101 tactggatta gatgagtata ttgagacagg aattgcatta gatgcagata tcactggaga
     5161 gctgctgatc aatatcttta ttcctaatac accgctgcat gacggtgcag ttattgtaaa
     5221 agaaggaaag atcgcagtag caagcgccta tcttcctcta tcagaaagta tgttgattcc
     5281 aaaagaattc gggactcgac accgggcagc tgtcggtata agcgaagtca gcgacgcgat
     5341 taccatcgtt gtctctgaag aaactgggga tgtcagcatt acattagata atgaactgat
     5401 ggctggctta tcccagcaag aatatttggc aattttgcgt agagaactga ttgctgaaga
     5461 gaaaacaaaa aacaagaaaa acgtgattca atctttccta gacggtatga ctaagggagg
     5521 ggatcgcaaa tgatctcaaa agagaaacgg acaagcattc tttacagttt tattgcattg
     5581 ctgttcagta ttgcgctgta cttcaatgca aatggccagt ccgttccaaa tacgctttct
     5641 ggaaatgaag cgtataatca aacagttact ggggtgccaa tcaagatttc ttatgattca
     5701 aagcgctact atatccatgg atatgaaaat gcagtgactg tcaaactttc cagtgcaaac
     5761 cgagttcagt tgaatgcaga ggcaaacgaa gatacacgga tgttccgcgt cacagcagat
     5821 atcactaagc tgggcgaagg aacacatgag gttccgctaa aaattcaaaa tttgagcagt
     5881 gctgtcacag caaagatcga accatcgact attacagtaa cggttgagaa aaaggtaata
     5941 aaagatttcg atgtggaaac gttgattcct tcaacgttca caccatctgg atatgaactt
     6001 gatgatactt ccgttagtcc taaaaaagtc tctatcacta caggtgacaa aacattggca
     6061 gaaatcaaac gagtagtagc tgatgtggac gcttcgatgg tcacagatga tgggattaat
     6121 agtgaggtcc caatacaggc gctgaatgca gcaggggaag tattaagtat cgtctctgat
     6181 ccagtgcaag taacagtgag agctgattcg atcaagccaa gcaagtcggt acgactttat
     6241 gggatacagc aaggaacacc agctgccggg gtagaaagct atgatttcag tttttcaaaa
     6301 ctggaagcag aagtaagcgg ctctagcgat ttgcttgcat cgatcggtga cagtattgct
     6361 gtaccgatca atgtctcagg tatcaatcat cgaacaactc gtaaaattga aataccagta
     6421 gaagaggggt taagtatcaa gcccgaatca gttagtgtgg agattacacc cgtgttgcaa
     6481 acgtcaagca gtagctctgc ttcgacgagc agtcggacac aagaaaatac aagtacgagt
     6541 agaagtattt catctgctga ttcaggaacg gaaccatcga caacaaccat ttcttccagt
     6601 tcggaatctg ggacaagcag ctcgaccaca agtacagaag aaacatcaga aacaagcgaa
     6661 tcaacacaaa tgagcagaac aaattcctca gaaaattaag ttaaaggagt aattttacaa
     6721 ctatgggtaa atattttgga acagacggcg tacgcggtga agccaacaag gaacttacgc
     6781 cagaacttgc atttaagctg ggacggtgcg gaggttatgt cctaagccag catgaagaag
     6841 aaggaaaacg cccacgcgtg ttagtagggc gggatacaag aatctcaggt caaatgttag
     6901 aagaagcgct gatttcagga cttttatcgg tgggtatcga agtgttccag ctaggtgtga
     6961 tctctacacc aggtgttgct tatctgacac gtttgcaaaa agcaagcgca ggggtcatga
     7021 tttctgcttc tcataatcca gcacaagata atggaatcaa atttttcggt gcagatggtt
     7081 ttaaactagt agatgaccaa gagctagaaa ttgaagcatt attagatgct gaagtggatg
     7141 aattgcctcg tccatcagct gaaggattgg gaactgtaga agaatttcca gaaggcttat
     7201 tgaaatattc tcaattcctt gtacaaacga tcaacggtga cctttccggt ttgaccattt
     7261 gtgtcgatgc agcaaacgga gcaacttcaa caagtgttaa tcgtttgttt gccgatcttg
     7321 aaacagattt ttataccatg ggcacaaatc cagacgggtt aaatatcaat gatggcgtag
     7381 gctctaccca tccagaacgt ttagctgaga tggtcgttga aaaaggagca gatgctggac
     7441 tagctttcga tggcgatggc gatcgtatta ttgcggtgga tgaacttgga aacattgtcg
     7501 acggtgataa aatcatgttt atctgtgcta aatacttggc agaaaaaaat cgattgaaaa
     7561 aagatacgat cgttacgact gtcatgagta acttaggatt ccacaaagcg gtggaaggaa
     7621 tcggtttgaa agatgtcgtt acacaagttg gtgatcgtta cgttgtagaa gaaatgcgca
     7681 aaaacgatta taactttggc ggcgagcagt ctgggcacat gattttctta gattttaaca
     7741 caactggaga tgggatgctt tcaggaattc agttactgaa tatcatgaaa cagacaggta
     7801 agaaattatc agagcttgca gcagaggtaa caatctatcc gcaaaaatta gtgaatatcc
     7861 gagtaagtga taaaaacggt gcgatggatg ttccggcaat caaaaaagtg attgaagagg
     7921 cagaagctga aatgggatca gaaggaagaa tcctagttcg tccttccggt acagaaccat
     7981 tacttcgagt gatggcagaa gcgcctactg atgagaaagt taattactat gtcgataaaa
     8041 tcgcggcagt agtcaaagaa gaaatcggac tggattaaga gatattttca taaaaaataa
     8101 aagaacaagg accagtgtca gatgtctgac actggtcctt gttcttttat tattctactg
     8161 tcactgattt cgctaaatta cgtggtttgt caacgtcgaa tccccgtaat agcgttgcgt
     8221 aatatgcaag taattgagtc ggtacgactg tggttagagt agttagataa gggtggacag
     8281 ttggtagcac cagctggtca ctttcctttt ctaatccctc tgagacaata actagtgttt
     8341 tagcgccacg gctttcgact tctttcaggt ttcctcgagt atgcgcacca gtcacttctt
     8401 cactgatgat tccaatcact ggtgttcctt cttcgatcaa agcaatcgtt ccatgtttca
     8461 attcgccagc ggcaaaacct tctgcctgga tataactgat ttctttcaat ttcaaagcag
     8521 cttcaagaga aacgttgtag tcagcgccac gtccaatgta aaaggcattt cttgtatcgc
     8581 tgagatatac ttctgccaac tcttctaaat aatctttttg ggcgatgacc gattccatcg
     8641 cattagcaac taaactcaat tcatgagtga tatcgaatac aagagaagtt tcaaattctt
     8701 ttttatcacc gatagctttg gctaataaag ttagtacagc tatttgtgca gtataagctt
     8761 tcgttgatgc tactgcgatt tcgggtccag catgcaatag cagtgtaaag gatgcttccc
     8821 gcgaaagagt agaaccagca acatttgtaa tcgtaagtga agggtagccg agacggttga
     8881 tttttaccag aacttggcgg ctatctgctg tttcgccact ttgactcaag aaaataaaga
     8941 atggtttttt tgaaagaaga ggtgtgttat agccaaattc actggctaaa tgaacctcaa
     9001 cagggatctg tgtcaatttt tccagagtct gtttacctgc taaccccgca tggtaactag
     9061 ttccgcaagc aacgatatag attcgatcgc tatcagacaa tgtatcgacc agcttttgat
     9121 caagagagac acgattgtct tctgttacgt atttttgagc gatacgacgc atgataatag
     9181 gctgttcgtc gatttctttc agcatataaa aaggataggt acctttctcg atatctgaag
     9241 aatctacttg tgcttcgaat gaagaacgtt ctacgatgtc acctgcaaaa ttttgaataa
     9301 taactttttc tgatgtgacg gtaaccatct cttcatcttc aatctcaacg aattccttcg
     9361 tatgagcaag catagccatt gcatcgctgg caatcacatt gaatccatcg ccgagtccga
     9421 ttaatagtgg acttttattt ttagcgacgt aaataacatc aggtgctgtc cgatcaatca
     9481 aagcgaaagc gtaagaacct ttgatgaccc gcaaagcttt taaaaacgcc tcttgcgttg
     9541 tagtatcctt cgcgaaagtt tcaattagat gagcgataat ctcagtatcg gtctcaccaa
     9601 taaagtggtc atttgctaaa taagcaattt taagttcttc aaagttttcg attacgccat
     9661 tgtgaacgag cacgaagcgt ccactttggg aagtatgtgg atgggcgttt tcttcagaag
     9721 gttgcccgtg agttgcccag cgtgtatgac caatacctat atttcctgta gtatcaatat
     9781 ttattttgtt tttcaaattt tgaattctgc cttgtgcttt gaccagataa tcagttgtgc
     9841 catctgagac aaagatacca gctgaatcat aaccacggta ttctaatttt tctagaccgt
     9901 caatcaatcc tggtgttaca ttttttaatc caatcattcc aacaattccg cacataaaaa
     9961 acatcctact ttctataatg aattcgtatg aatgcttcca gctgcaggaa aatccttttg
    10021 ttttatcttt gcagatattt tttgtggatt ttctgtagag tgcagtccct agggccatcc
    10081 gccgaatctt tcgataaacc ctctcctcgt catctgttta cacagacctg gcgctatcct
    10141 aaaaggtata actatcctcc tcttttgttt tttatctgga aacatttaaa atcataacct
    10201 tttgcgaatg ttcttgtcaa caaaaaaaga ataaaaaata aattggtata tatcaaatgc
    10261 tttgtcaaat gctcttttta tgtcaaaaaa taatgggttt ttatctatca tctttttcag
    10321 gagttcggta caatagaaag taatcggata gaggaggagt aaaaatggat tcaaaaattg
    10381 agatcatgac actgggaatg ttaaaaaaac aattgtccaa attcgaagca tcagctggtg
    10441 tatcagatga tacgaaaata tttttagata ctggctggga tagcatccaa gaaatcgcac
    10501 ctgatgcctt ggaagttgct gaagctcgtg agtttacggt agaagatgaa ttgacaaaag
    10561 agtcgttttc cgggtatgcg cgggaagaaa aagcagagcg gttcgacact tctgagcagt
    10621 cagaaactgt gatcgtcata aaaaacttgt attagaaagg agaaagaatg aataagagac
    10681 gttgggcagc agttctgatt gctgcaggat tatttgtggt ttcgttagtt tcagctagtt
    10741 tgactaagcc tcaggaaaac gatcaaattg agggattgaa cagttggtta tatggggatg
    10801 aagaactagc ttcaaatgtg ttggaagagg gagacagtac gcaaaaaatc gtaaaattat
    10861 cagtagatgg aacgattgct gcgggagggt cttcaggttt atttgcttct gaaggttaca
    10921 atcataaaaa ttttatggaa caactgaagg cgattgaaga agatccaaca gtcaaaggta
    10981 tctttttaga agtaaattct cctggtggcg gtgtctacga aagtgcggaa atcgctaaga
    11041 aattggatac gattcgtaaa gaacatgata tcccaatgta tgtcagcatg aaaaacatgg
    11101 cagctagcgg tggctactat atttctgctc aagcagataa aatctttgca acagaagaaa
    11161 cagtgaccgg ctcaattggt gtgatcatgt caggattgaa ttattcagga cttctagaaa
    11221 aactgggtgt ggaagataca acagttaaaa gcggagcgtt gaaagatatg ggttcaggaa
    11281 cgcgaccaga gacaaaagaa gaagaagcgg tgcttcaagc atatatcgac agcgcatata
    11341 gtcgttttgt agcaatcgtc agcgagggac ggaataaatc tgaagaagaa gtcaaaaaaa
    11401 ttgctgatgg aaggatttat gatggtgtcc aagcaaaaga agtaggttta gtcgatgaat
    11461 tgggatttcc tgaagatgct ttaaaagcta tgcgaaagga acaacagctt gaagatgcgg
    11521 aactagtaga atattcttcc acgccatctg ggtttgctag tacatggtta ggaagtaaac
    11581 tagcagaatt ccagggattg aagccttctg agactagtca gatcttatcc gttcttgaga
    11641 atctagggac agcggaatca ccaaaagcaa tgtattatta cggaggtgag taagcatgtc
    11701 agagctttca aatgaaaata aggaacaaga aaaagataag caacagcagc ctgctgtagg
    11761 acctgactcc aacttttccc aaaaggtact gagctctttt gaaagagagc ctttgacgcc
    11821 cgaagaaatc aaaaagaaac aacgtaaatg gaaaaagtat acggacacaa gaaatgaacc
    11881 gccaaaagca aatgattttc ctaagtattt ctatgcaggt ttttttatcc gaatgtttgc
    11941 tttcttggtc gatcttttgt gtatcggagc tattacgcgt ttaacacttg gtattgccaa
    12001 aaatctaggt tggcttaatt tttcagattc ttatctgagt atctacggat tcacagcctt
    12061 acttatttat ctagcgtatt ttattttatt gacgaaattc aatcatggtc aaacgattgg
    12121 gaaaatgatc tttggtattc gagtcatcag ctttgatgaa gaagaactta gctggtcaac
    12181 cgttttagtt agagaaggtg cttgccggtt cattctaaaa tttcctttgc tgatgttagg
    12241 gtatcttcca gcaatcttta atcagaaaaa gcaacatcta ggcgattttt tcactaatac
    12301 aagtgttgtt acgatcaatc tgatcaaagc attcaaccag gaagtcaaca gctgatgagc
    12361 aataaaaagc agtaacagtt gaaagcacct tctaaaagtc agatctaaaa tctagctttt
    12421 agagggtgct tcagttattt ttaattgtca tgcttttttt gttttgctac tattgaacat
    12481 gagctgaaca tgagcaagag gaggacactg cgtgaagccg attgattttc cagaaaaata
    12541 tgagaattta atgcgagtag cacaacaagc actagcaaac cagcaatatc agcaggcaaa
    12601 agaattattt caacgagctt acgagttgaa agaaagcttt gaggctaata gcctgcttgt
    12661 tttttgtctt tacgagttgg acgagaaaaa agaagcattg aagcaagcac tcttgcatga
    12721 aaaacagtat cttcaaaatg aggagtttgc tgaattttat tttgacttgt taatcagtac
    12781 acaagatttt ttatatgcta gaaaattgat agcaagtaca gatttttatg aagctttcga
    12841 gcagcgggtc attgaaaaaa tccaatttgc agaagaatta tccggacaaa tggagaggca
    12901 gaagatcaag gcactccaca ataaatcgga ggaacttcct tcgttggaac ctgcacgtca
    12961 gcttgcgtct attgatgaga tcgaacagct tccttatcat gagtttatcc aaacagcgag
    13021 taaacttatt gtatcgccag aggtccatat tttagcaagg gcaaaattac tggagacact
    13081 taggcaattg aacgaatgcc atcctgtttt ctatttgacg attgaagaaa agttagtcaa
    13141 agtcatacca aaagatctgc caaagccgca gcagcaggca agttaccagc aattatgtgt
    13201 attttcagat cgttacggta atgaagatgc gctgcttagt tccgtgttaa atgaagaatt
    13261 cacgttgcaa tctgcaattg tttatcctgt ctatgataca tacatcgaag acccgaagcg
    13321 ctggtttcaa ctgactgtag aggcgtgtac aggaaaaacg atgtatgaag gtacagaaga
    13381 tgaaaaacaa gattttttaa aaaaaagaga aaaaattttg cagcaaatga tcttcttcca
    13441 ttagatttta tcagtgaaaa agataaaaat gttatgaaaa acaaggattc tatccattca
    13501 atatgtttac aatttgaaag ggttagtgta ctatttaaag gtatgcatga aacgtttttg
    13561 atgtataaaa tgaatttcta aactcggagg gaattaaatg actgcaacat ttgaaaaaaa
    13621 aggcacgaat gatggtgttt taaaatttac gattgcgcaa gacgaaatcc aaaaaggatt
    13681 aacaactgcg tttaataaag tgaaaaaaag cttgaacgta cctggattcc gtaaagggaa
    13741 agtttctcgt caagtattca accgtatgta cggtgaagaa gctttatatg aagatgcatt
    13801 gaatgctgta ttaccagaag cttatgaagc tgctgtcaaa gaagctggca tcgatcctgt
    13861 ttctcaacca aaaatcgatg ttgaaagcat ggaaaaaggt caagactggg taatcactgc
    13921 tgaagtaaca gtgaaaccag aagtaaaatt aggacaatat aaagatctag ttgttgaaaa
    13981 acaagaccgt gaagtaacag atgctgacgt ggatgcacgt atcaaacgtg aacaagaagc
    14041 acaagctgaa ttagttatca aagaagatgc tgcagctgaa aatggcgata cagtagtaat
    14101 cgacttcgaa ggctttgtag atggcgaagc gtttgatggc ggaaaaggcg aaaattactc
    14161 acttgaatta ggttcaggct cattcattcc aggttttgaa gatcaattag ttggtcataa
    14221 atcaggtgaa agtctagatg taaatgtaac tttcccagaa gattatcaag cagaagatct
    14281 tgctggaaaa gaagcagtct tcaaagtgac aatccatgaa gtaaaagcaa aagaagtgcc
    14341 tgaattagat gacgaattcg caaaagatgt tgatgacgaa gtatcaacat tagacgaatt
    14401 gaaagaaaaa tatcgcaaag aattagtaga aacaaaagaa aaagcagcgg acgatgcaaa
    14461 agatgaagca gcgatccgta tggctgttga aaacgcagaa atcgttgaat tgccacacgt
    14521 gatggttcat gatgaagtac atcgttcaat ggacgaattc ttgaacaaca tgcaacgtca
    14581 aggtatctct cctgaaatgt actatcaatt aactggttct acagaagaag acttgcataa
    14641 acaattcgaa ggtgaagcag aaatgcgcac aagaacaaat cttgtgatcg aagcaattgc
    14701 agctgctgaa aatcttcaag caacagaaga agaaatcaat gcagaaatcc aagaattatc
    14761 agaaacatac aacatgccaa tcgaacaaat caaacgcgtg ttgacagaag atatgctgga
    14821 acatgatgtg acaatgaaaa aagcaatcga attgatcaca ggtacagcga cagaagcgaa
    14881 agaatagaaa tcaagtaata aatcatttct gaagaagcta cttctagaaa attgtttatt
    14941 cgaaataagg gactgtgaca cgattttgtc acagttcctt attttttgtt aagagtaaaa
    15001 aatgcaaagg tataggtcag ttgatccttt gttaaacact ttttcttttt actaaactat
    15061 gttatgatag taaaagactg taaagtcttt gatagtttct tgcatacttt ttgcaagtta
    15121 aggggtgaag accatgtatg acaatccaag tagcaatgag accgtacgct gttctttttg
    15181 cgggaaaacc caagaagaag tgaaaaagat cgtagctggt ccaggggtct acatctgtaa
    15241 tgaatgtatc gacctatgta aagaaatcat tgatgaagaa ttttatgaag aagctgtacg
    15301 cgaattcact gaagtcccta aaccactaga aattcttgaa gtattgaata actacgtgat
    15361 tggacaagat cgcgcaaaac gctcgttagc tgttgccgta tacaatcact ataaacgtgt
    15421 caatcagcaa gcacaagaaa acacagatga tgttgaactg caaaaaagca atatctgctt
    15481 gatcggacct acagggtctg gtaaaacatt tttagcacaa acattagcaa aaacattgaa
    15541 tgtacctttt gcaatcgcag atgcgacaag tttgacagaa gctggttatg tgggggaaga
    15601 tgtagaaaac attttgctta aattgcttca atctgctgat tacaatgtgg aacgtgcaga
    15661 aaaagggatc atctatattg atgagatcga taaaattgcg cgtaaaagcg aaaatgtatc
    15721 tattactcgt gacgtttccg gcgaaggtgt ccaacaagcc ttgctgaaaa ttctagaagg
    15781 aactgttgca agcgtgccac ctcagggagg acgtaaacat ccacatcaag aatttattca
    15841 gattgatacg acgaacatat tgttcatcgt aggtggtgca tttgatggca tcgaaacgat
    15901 tgtgaagaat cgtatgggcg aaaagacgat tggatttggt acacaaaacc aaaaactttc
    15961 tgaagacgaa agtgtcatgc agcatatcat tcctgaagat ttactaaaat tcggattgat
    16021 ccctgagttt atcggacgat tacctgttat ggcagcttta gagaaattga caacagatga
    16081 tctggtacgg atcttgactg agccgaaaaa tgcgctagtc aaacaatatc aaaaacttct
    16141 ttctctagat aataccgaac ttgaattcga acctgaagcg ttgagagcca ttgcgaaaaa
    16201 agcaatcgaa cgcaataccg gtgctcgagg acttcgttcc atcattgaag aaatcatgat
    16261 ggatgtgatg tttgatatcc cttcaaatga aaacatcgaa aaagtgatta tcacaaaaga
    16321 agcagctgaa ttgtctggtg agccaacagt aatctataaa gattcagaga aaaaagcggg
    16381 ttgatgaaaa gaggctgccg taaaagtcat tttggctttt gcgacagccg cttgctttat
    16441 tcatcacctt gttaaggaga gagaccatga aagtccatca agctgaaatt atcataagtg
    16501 cagtggcacc aaaacaatat ccagatacac aattgccaga aatagctctg gcaggacgat
    16561 caaatgtagg gaaatcttct tttatcaaca cattgattga tcgaaagaat ttagcccgta
    16621 cttccggtaa gccaggaaag acccagacat tgaattttta tttgatcgag aacgcattac
    16681 attttgtcga tgttccggga tatgggtatg caaaagtatc caaaactgag cgagcaaaat
    16741 ggggaaagat gatcgagaca tatttaacga ctagagacca gctacgtgcg gtcgtttctt
    16801 tagtagattt gcgtcataag ccttctcaag aagatatcca gatgtatcaa tttttgaagt
    16861 attatgatat ccctgtaatt gttgttgcca ctaaggcaga taagatccct cgtggtaaat
    16921 ggaacaagca tgagagcgaa atcaaaaaag cgttggattt tg
//
LOCUS       DBNICI010000051        16867 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.051, whole genome shotgun sequence.
ACCESSION   DBNICI010000051 DBNICI010000000
VERSION     DBNICI010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.051"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(120..733)
                     /locus_tag="AC1ZCC_001355"
                     /pseudo
     CDS             complement(120..733)
                     /locus_tag="AC1ZCC_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(869..1063)
                     /locus_tag="AC1ZCC_001356"
     CDS             complement(869..1063)
                     /locus_tag="AC1ZCC_001356"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629618.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(1113..1553)
                     /locus_tag="AC1ZCC_001357"
     CDS             complement(1113..1553)
                     /locus_tag="AC1ZCC_001357"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIQ1629619.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(1557..2402)
                     /locus_tag="AC1ZCC_001358"
     CDS             complement(1557..2402)
                     /locus_tag="AC1ZCC_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HIQ1629620.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            2551..3969
                     /gene="pepV"
                     /locus_tag="AC1ZCC_001359"
     CDS             2551..3969
                     /gene="pepV"
                     /locus_tag="AC1ZCC_001359"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HIQ1629621.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(4026..4973)
                     /locus_tag="AC1ZCC_001360"
     CDS             complement(4026..4973)
                     /locus_tag="AC1ZCC_001360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIQ1629622.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(5196..5546)
                     /locus_tag="AC1ZCC_001361"
     CDS             complement(5196..5546)
                     /locus_tag="AC1ZCC_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIQ1629623.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            5746..6825
                     /gene="pepA"
                     /locus_tag="AC1ZCC_001362"
     CDS             5746..6825
                     /gene="pepA"
                     /locus_tag="AC1ZCC_001362"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HIQ1629624.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            6969..7289
                     /locus_tag="AC1ZCC_001363"
     CDS             6969..7289
                     /locus_tag="AC1ZCC_001363"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIQ1629625.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            7311..7775
                     /locus_tag="AC1ZCC_001364"
     CDS             7311..7775
                     /locus_tag="AC1ZCC_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIQ1629626.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            7980..8585
                     /gene="ytpR"
                     /locus_tag="AC1ZCC_001365"
     CDS             7980..8585
                     /gene="ytpR"
                     /locus_tag="AC1ZCC_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HIQ1629627.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(8624..9913)
                     /locus_tag="AC1ZCC_001366"
     CDS             complement(8624..9913)
                     /locus_tag="AC1ZCC_001366"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIQ1629628.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            10341..10820
                     /gene="rlmH"
                     /locus_tag="AC1ZCC_001367"
     CDS             10341..10820
                     /gene="rlmH"
                     /locus_tag="AC1ZCC_001367"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HIQ1629629.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            10905..11414
                     /locus_tag="AC1ZCC_001368"
     CDS             10905..11414
                     /locus_tag="AC1ZCC_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HIQ1629630.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            11514..12164
                     /locus_tag="AC1ZCC_001369"
     CDS             11514..12164
                     /locus_tag="AC1ZCC_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629631.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            12615..13553
                     /locus_tag="AC1ZCC_001370"
     CDS             12615..13553
                     /locus_tag="AC1ZCC_001370"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HIQ1629632.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            13566..14618
                     /locus_tag="AC1ZCC_001371"
     CDS             13566..14618
                     /locus_tag="AC1ZCC_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIQ1629633.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            14884..15519
                     /locus_tag="AC1ZCC_001372"
     CDS             14884..15519
                     /locus_tag="AC1ZCC_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HIQ1629634.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            15710..16501
                     /gene="msrA"
                     /locus_tag="AC1ZCC_001373"
     CDS             15710..16501
                     /gene="msrA"
                     /locus_tag="AC1ZCC_001373"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIQ1629635.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT         5367 a         2944 c         3405 g         5151 t
ORIGIN      
        1 taaacggtgt gcaatcagct gaccctcggt attagctcaa aaaaaacggc aaaatatgag
       61 gaactatttt gccgtttctt tctctaatac tcaggtcaaa acgctgattc caaaacctct
      121 tatttgatgg gtctgtcctg aatatcttca aaataatcgg ggtaattatg aatcacgtat
      181 tcttgctcct cgataatcat atgtaaatgc atcgtgcaaa gaaatccgac ttcatcaaag
      241 ttatgatcaa tcattgattg aagcattgtc tgatgttcat ctaatactcg accccagtct
      301 aattctgaaa tcgtcaaacg taaccatctg aaacggttca agtgcgtact ataactttct
      361 agccaatccc aaatttctcg ttttccagca atttcataac aagttcggtg aaaatcagta
      421 tccaaatcaa aaaaagcttt tttattttga gcgattactg cctgatcggt ttttccaact
      481 tttcccgcgt ataacgggct aatgaagcag atcggatatc gatttttgaa acataagtgc
      541 cggactgtgg aatggttttt acaagctctt gattgcgcag ttggatcagt gcttctcgaa
      601 tcggtgtacg cccgatagct agttgttctt ctaacacttt atcggagatt ttacttccag
      661 gtgctaactc ggaataaatg atttgtttac gtatagattc ataagcttgc tgttgcaaat
      721 tattgataac catttaacct tcctttctat gcagatataa tctattataa cataaatcgc
      781 ttacaaatcg tgtgggcata aaaacacagt ctgcaaaaaa agccctgata ggcatgccta
      841 ttagagcctc gaaaagaaaa atattgaatc aatctggtcg tacctcttgt atcggtttga
      901 cacgaccgcc gattctttta gcaaaagctt cggattcttt tttgtctttg aaaatgagca
      961 gattgtctaa gtttttttcc cgagtgcgac agccagtttt atcgatgtaa ccgtgcatta
     1021 ctttaacgac atacatgggg atcacctgct tttttctttt cattatacga actactcgac
     1081 acaatacaag aaaaatgctt agacggtctt tattaagaat ttacatcatc caaagaagcg
     1141 tacgcttgga ataactgatc taactccgga ttgactttgc tcagcttcac taatcgttgc
     1201 gaatcagaaa gtaaaaaaca gtgtttacta ctgccagtag tttttaattt tttgtcttca
     1261 gttcccacaa tttcgtagct aaaatccaac cgtgtccccg tatacttttg gatagttgga
     1321 tggatgctca ctttttctcc gtaatggatc atttctttat agctgcaatt tacttccaga
     1381 acggggatga tgatcccaga ttcttcgatt tttttatagg gaaaccctag atgtaccaat
     1441 aaggctaccc gagcctcttc aaaccagcgg atataattgc tgtgatgaat aatccccatt
     1501 tgatctgttt cataataaaa aggttctcgc acataacctg taaatttctc catgactcat
     1561 gcctcgtttt ccattgattt tttacaaaaa taattcatcc atttcggaag tgcttcgctg
     1621 atttgtgtcg gcaaaacaat atattgttct tttgccagct ggtctccaat gaaactatga
     1681 agataaatag ctgctgcaat cgtttggtca ttttgaggaa actgggctaa aaatcctgtg
     1741 atcatcccag ctaaagtgtc acccatgccg cccgttgcca tagcagcaga acctaaagga
     1801 ttggtgtaga agtgctggtg accataaatt tttgtctcat gacttttcaa aacgagcaca
     1861 gcaccgattt cctgttgctt ctttttgttt tctttatccc gttgttcttg tatcggtaga
     1921 gaactcaacc gttgccactc catttgatga ggtgtaagta cagtttgttc gggatagggt
     1981 agcaaaagat gattagcagc aagtaaactt aaagcagagc catcaatcac cagccattgt
     2041 tcttttgatt ggttttctag tacttgtttg aaacgttcca aactcttaga agaagtaccc
     2101 aatccgggac caatcaagag tacatctgct tgctttatta cggaaagaaa ctggtcagta
     2161 tccgtccaaa ctacaatcat tgcttctggc agatgactgt gaagagcact ccgatttttt
     2221 tcatcagtta ctacagtcgt gagacccgct ccgctgtgta cggcagctaa agcactcata
     2281 atgattgctc cgccatattg ttcattgccg ccaatcaaaa cgatacgtcc aaatgttcct
     2341 ttatgtgaaa tttttggacg aggtgtgatg actcttttca ctagttgttc gtttatcagc
     2401 atctaaaaac ctccttttac tcattatagc gtatattttt tttagagaaa aacagataag
     2461 aaaaggaatt gtcagatttt ttgaactatt tttttctgta atttgtggta tcatgaacac
     2521 gaagacttaa ataagtggag gattaacttt atgacaattg attggcaaaa agaagtagaa
     2581 gcacgtaaag aagacttgct tgaagacttg aaaaatcttt tacgtgtcaa cagcgagcgc
     2641 gatgactcaa aagtaacacc agatgcacca tttggacctg gacctcgcga cgcattgaaa
     2701 catatgttag cttatggcga acgtgatgga tttaaagtaa aaaatgtcga taactatgca
     2761 gggcatattg atcttggcga aggagacgaa acgctaggga tatttggaca catggatgtc
     2821 gttcctgcag gagatggctg ggacacagat ccttatgaac cagtaatcaa ggatggaaag
     2881 atctttgcac gcggatcaag cgatgacaaa ggtccaagta tggcagctta ctatgcgatg
     2941 aagatcatta aagaacttga cttgaaacta tcaaaaaaag ttcgcttcgt agtcggcagc
     3001 gatgaagaaa gcggctgggg cgatatggct tattatttcg aacatgaaga agaaccagat
     3061 tttggtttct caccagatgc tgaattccca atcatcaatg gtgaaaaagg aaatgtttct
     3121 ttagctttac gcttcaaagg cgacaatgca ggagattatg tcttgaaatc ttttgtttcc
     3181 ggacttcgcg aaaatatggt gccaggaaca gcaactgcag cacttcaagt accaagtgca
     3241 gatgcagcta tcgcgatgga agaagcattt taccaattca tcgaagcaaa tccagtaagc
     3301 ggaacgattg aagcggacaa tacgtatgta aaaatcgaat tagttggtaa aggcgcacat
     3361 ggtgcaagcc cacaatctgg tatcaatgca ggttctttct tggcattgtt cttagataac
     3421 tatgaattcc taggatctgc taaacaattt atccatgtag cagctgctta tgtccatgaa
     3481 gatttttatg gagaaaaact aggagtcgct tatgaagatg aaaaaatggg caaattgaca
     3541 atgaatgctg gactatttgc ttttgaagaa aatggtacag aggaagcgaa cttcatcaat
     3601 atgaatttcc gttttccaaa aggtgtaaca gtagacggat tgcaaagtga catcgaacaa
     3661 acagtggggc aagaaggcgc aacagttact cgaggtgctc gcgtaatgga accacattac
     3721 gtaccaatgg atgatccatt ggtggctaca ttattgcaag tctatgaaga ccacactgga
     3781 gaaaaaggct acgaacaaat catcggtggc ggaacttacg gacgtttgtt gaaacgtggc
     3841 gttgcttatg gagcgatgtt cccaggatat actgatacaa tgcatcaagc aaacgaattt
     3901 atgagtcttg atgacttgtt cagagcaaca gcaatctatg cagatgccat ctatcgtttg
     3961 gcgaaataat taattaaaaa gaggatcaag aattgcaaat tatttttgca gtcttgatcc
     4021 tttttttact ttctactgtg gtattcatga taaacatgat atagattcaa ttcatattct
     4081 ttcttagcgt tcgtgacgac tgtatccaaa atcaaccctg ttactaatga aagaagtgct
     4141 aaagtcatca atccagtact taaaatcgca gaagggatgc gtgtaataaa gcttgtcagc
     4201 atatattcgc gaatcacagg aatccccgtg accaatccaa ataaaaagaa gaagatggtc
     4261 caaatcccaa agaaaagcaa aggcttataa tctttaaaca ttttaataat catcatgatc
     4321 actttgaatc cgtcagagaa tgtattcaat tttgattcgc tgccttcagg acgatcacga
     4381 taatcgattg ggatttcaac gaacttgaat tttttatcta aagcatggat cgtcaattcc
     4441 gtttcgattt gaaaaccaga actcataata ggaaaacttt tgacaaagat acgattgaat
     4501 cctcgatagc cggtcatgac atcaagaatt tttgttttat acattctagt aattgtattt
     4561 ctaacaaggt tatttccaaa atcgtggaaa ggtctcttat tctcatcaaa gtaggttcca
     4621 tttgataggc gatcaccaat gaccatatct gcttcaccag aacgtaattt ctcgagcaaa
     4681 ccatgtacag cttctgcagg ataagtatca tcgccatcta ccattaaata atagtcggca
     4741 tcgatatcaa agaacatttg gcggatcaca tttccttttc cttgacgagg ttcttttttt
     4801 acgattgctc caccttgaac ggccaattcg tatgttcgat cggtcgaatt attatcatag
     4861 acataaatat ctgcttctgg taattctcgt ttaaagtctg caatgactgt tgaaattgtt
     4921 gcttcctcat tgtaacaagg aatcagaaca gcaatttttt cttgttgttc catttttcac
     4981 tctccttaac tatagaaaac aaaagctaaa ttaaaaatac ctcataatca aaggaaatgc
     5041 tagtttttcc cgctaaatat catttattta aaaagaaaat actggaatat atgtttggaa
     5101 agctctatct aaaaaaccac ccactacaaa agaaggacat gatacctctc ttttgaatag
     5161 tgggtgcgta ttcgttctct cacttttttg acgtattaat ctgttgcacc tttttttctt
     5221 gaaatttcta atacggttcc tgtatgagca tctgctaaaa attcataata aacagtttcg
     5281 ccatcttcta ttcgagaaag acctccccga taagcactcg accgaacggc gaatttccta
     5341 ataggttcct tttcaaaaga gatccaagaa ccttcgattg caccttcttt tagaaaagat
     5401 gacttgatat tttcaagtac ttggtctgct gacaatactt ttttgcgatg ataccaaatc
     5461 gttgaagcta ccccgctcaa taatcctaga ccgattccaa ctgctaaccc gcctttaaaa
     5521 tagcttagtt cgctttcttc atacatgtgg acatacctcc ttcttctcac ttgttactcc
     5581 ttgtgcttat tttaccacac tctcatattt tctttgaaat aaataaccgc taccagaaaa
     5641 aataagctat aataaatacg atcggtctgc agaaaaaatc atattttatc cgcagatcaa
     5701 tataagtctg agaggagttt agtctgaaat ttagacgaat agcatatgga agaaaaaaca
     5761 tttcaacgaa tcaaagagtt aacagaatta caaggaacaa gcggttttga acaagatgtt
     5821 cgcgcttata tggaaagtca tatggaaccg ctggtagatg agttacagtt agatggtttg
     5881 ggcggcattt ttggtctgcg ccatcatgaa gaagcggatg caccccgtgt aatggtcgca
     5941 gcacatatgg atgaagtcgg attcatgctg acacaaatcc aagaaaacgg gctttttaaa
     6001 gtcgtacctc taggcggctg gaatccttac gttgtttctg ctcagcgttt tacacttaaa
     6061 acctctactg gtaagaatta tccatgtatt tcttcttccg ttcccccaca tttattgcga
     6121 ggaacaagcg gacaaaaatc agtagaagta accgatattt tgttcgatgc tgggtttgaa
     6181 tcaagagaag aggcaatgag ttacggtgtc cttccaggag acacaatcgt tccttacgct
     6241 gaaacgatca aaacagccaa cggaaagaat attatcagta aatcatggga caaccgctat
     6301 ggctgtacaa tggtattaga agctttagaa gcgttgcaaa atgaaacatt aggtcataca
     6361 ttgattgcag gcgcgaatgt ccaggaagaa gtaggcttgc gtggttctaa agcttctgtc
     6421 aataaattca agccagatct gttctttgca gtagactgct ctgcagcaga tgatacagtg
     6481 acgaaaaatg gcacattcgg acatttagga gaaggtacat tgatgcgtat ccaagatcca
     6541 ggtttgatca tgcttcctcg tttgcgcgag tacttgctgg atatcgctga aacaaacaat
     6601 atcccttatc aatactttgt ttcaaaaggc ggtacagatg caggtgctgc tcatacacaa
     6661 aacgaaggaa tcccaagtac agtgattggc gtagtgggac gttatatcca tacgcatcaa
     6721 acgatgttca gtattcgcga ttttgaagct gcacgagaaa tgttgattca aacactgaaa
     6781 ggtttggata aatcaacagt gaatacgatc gtatacggaa aataatgcga tagtgaaact
     6841 ataaaatctg gcaaaagatt gctttttgcc agattttttt tataaaaaga ggatcaatgt
     6901 ttgttagttc gaaagaaagg tgtataattt aattgaaaaa tacataaaca gaaagtagag
     6961 gacaaagaat gattatccca aatagtttag aagaattagc aggctacgta gaaactggaa
     7021 agagtgtatt tttctttact gccggctggt gcggagactg ccgttttatc aaaccgcaaa
     7081 tgcctgaaat tgaaaatgac ttttctgatt ggcaatttat tgaagtagac cgtgacaagt
     7141 acatagatgt agccgctgaa tggaacattt ttggtatccc aagttttgtc gtgatccaag
     7201 acggaaaaga attaggtcgt ctagtgaata aagatcgtaa aacaaaacag gaaatcgaat
     7261 cgttcctaga aagtgtaaca aaaggctgac atctgttagg aggggtcaag atgaatcaag
     7321 aatataagaa tattttagta ggtatcgacg gtagtgaaca agcacttgct gctttcaaaa
     7381 aagcggtaga agtggctcgc cgtaataatg ggaaagtata tgtagcaaac gtaatcgatc
     7441 agcaatttta taactttatg ggctatgcac ctattgatca aaatctagtc gatcagcaaa
     7501 ctgaaacagc caaggaaatg atcgaagaat gtaaagaata tgggaaatct gttgattaca
     7561 caaatataga aggaattatt gcatatggtt ctgttcgtga atccatggca gtcaaattgc
     7621 caaaaaaata tgagatcgat ctaatcatgg ttgggcagtc aggtctaaat gcagtcgaac
     7681 gctttatgac aggaagtgta gccagctacg tgatacgtca ttcaccttgt gatgtcttga
     7741 tcgtttctga cgaagaagaa cagccaagta attaagcaag tggaacttgt acgacctttt
     7801 taatcaatta actaaaacag taaagggact gtgacaaaag tcttgtcaca gtcccttgtt
     7861 acgaataaat ggtgttccag aagaggcttc ttcggaaata aagcatgaaa aaaatttcat
     7921 gcagtgaatg caattttttg ttaagataaa tctcagcaaa ggataatgga ggaaagaaca
     7981 tgatttttgc ttataacaaa gcacacgtcg gagacacctt gatggttatt atagccgacg
     8041 ataaaggaac agaaaacact gttgaacgca aatggaatgt tgctcggatc aaagatgaac
     8101 aaggtcaagt tgttgcttgg aactttttcc atatctccga tcatctaaca atcgaaggga
     8161 acggccaagt aagcttaaca gatgaggaat tagcgaaatt gaatcgattg atcaaagaag
     8221 caggattcga agaacagctt gaagctgatc atgaaccgaa gatcgttgtc gggtacgtaa
     8281 aaacatgcgt cccacatcct gattctgacc atttatcgat tactgagaca gaagtagaca
     8341 atggtacagt attgcaaatc gtttgcggtg cacccaatat cgaagctgga caaaaagttg
     8401 tcgttgcaaa accaggagct atgatgccag atggaatgat gatctggcca ggaactttgc
     8461 gaggggtaga tagctatgga atgatctgtt ccgctcgtga attgcatttg ccaaatgctc
     8521 cagcaaaaaa aggaatctta gtattgcctg aggatgccgt agtcggagaa gcatttccaa
     8581 aataaagagg ttgtgacaag tctttgtcac aacctctttt ttattaattt tcactatttt
     8641 gattttgttt taatgccgat tgatcgatcg tcaattcaac agtagttgtt ttttctttcg
     8701 agccttcata gtaagtgatt ttgacagaat ctcccacttt tttcttatac aaagcagatt
     8761 gaagttctac accagaagaa acttctttgt catcgatctt agtaatgacg tcgtattgtt
     8821 tcaatccagc tttttcagcc ggtgtagctg tttggacatt tgtcacgatg acaccgttag
     8881 ttacagatga aggaatcttc aagatttgtt cttgttgctg agtagaaacg gcagataagt
     8941 cgaccattgt gatacctaga gctggacgtg tcacttttcc gtctttttcc agttgattga
     9001 tgatattcac tacatcgtta cttggaatag caaatcccat tccttctacg cttacattag
     9061 aagtagattc agaagtactt gcgatcttac ttgagttgat accgataact tgtccttcga
     9121 tattaactaa aggaccacca gaatttccag ggttgattgc tgcatctgtt tggatcgcat
     9181 tgatattgac tgtttcgttc gattcattcg tacttgtcac ttgtcggttc aaggaagaaa
     9241 tgattcctga agtgacagaa tttgcatatt cagatcctag tggggaacca atcgcaatcg
     9301 caggctcacc aaccttcaga gcacttgagt caccaaaaga ggcaactgtt tcgactttgt
     9361 ctgaattgat cttcaacacc gctaaatctg aataagcatc tgttccaact aattcagctt
     9421 ttaccttcgt accgtctttc atcaagactt caagaccttg ctggccatct actacgtgat
     9481 tattcgtgac tacataagca gtattcccgt cttttttgta gatcacgccg cttccttcac
     9541 tataagcttc caaattacta tcatctgagc tgctactttc ttcttgctgc ccgaatagtc
     9601 cgccaaaacc gcttgattgg ttttgacttt gtaagttgat aacagaaacg acagcgtctt
     9661 gaactttatc taccgctttg gtgatatcag aatctacatt gactttgaca ttttccacaa
     9721 ctgtttcacc ggcagaattc tgattgccgc ttgtagcagt agaactattg ttgccagtgc
     9781 ccatcgctgc ataaaaaatg ccagctgtca gtagtcctcc aacgatccct ccaacaagcc
     9841 ccagacctaa cttacgccag aggctattat ttttactttt tttcatcttt ggtgtcacat
     9901 tttttctatc catgtgttcc tcctccaatt acattcattt atctactgtt agtatagtct
     9961 tatttgttga ctttagtcta ctttgagact gtgaactttt tatgaaaaac tttaggaaaa
    10021 atcattgttt tcagatgaaa atatttgatt ttctttttcc acagatactg tgtaaaacta
    10081 cgcggatagg attggaaaaa taagggataa aacatatcca cagcctgtgt ataaagtgga
    10141 taaattctta taaaacgtat gtttgcctaa tgaatgtgat gaaataaggt aaaataacac
    10201 tgttttctgt ggataaacct gtggatgttg tgcataactg ttggggaaat ctttttaaca
    10261 aaaatagcca aaataaacat atagacgaaa aaattgatta taataaacaa agtgagcatg
    10321 aagaccaaag gagagaaaac atgaatatca aaattatctc agtaggaaaa ttaaaagaaa
    10381 aatacctgat ccaaggtatc aatgagtatg tcaaacggct gaatgcctat gcaaaaatcg
    10441 aattgatcga agttcctgac gaaaaagccc cggagaattt aagcgaagcg caaatgagac
    10501 aagtaaaaga aaaagaaggg gaacggatct tagcgaaaat caaagaacaa gaatacgtct
    10561 atgccttagc aattgaaggg aaaaatccta caagtgaatc ttttgctaaa caaatcgacc
    10621 aattaggtat ccaaggaaaa agccaccttg tttttgttat cggcggctct ttaggactca
    10681 gcgaagccgt catgaaaaga agcaatgctc agatctcttt tggaaaaatg acgtatccac
    10741 atcaattgat gcgtctgatc cttgtcgagc aaatctaccg tgccttccgg atcaatgcgg
    10801 gggcgcctta tcataagtga tgcggttttt atcattgatc ctaaaaatct tagcaagaca
    10861 aaaagtacgt aagaaagtaa agactaaata cttcggagga acaaatgaat aacaatgata
    10921 ggttattgcg attgcgttat gcgattgatt tgaaagatac ggatttgatt aaagcttttg
    10981 agctaggcgg tgtaccactg actaaagaag aagctcaggc ggtactgacc aaagtacagg
    11041 ataagaacaa agataatgca gaaaataatg tgtatgaaaa aaccatcaac aaccaagttt
    11101 ttgacgcatt cctaaatggc ctgattctct tggcgcgtgg tccgcaaaaa gataagccga
    11161 tagtagacac gccgcaaaaa agcaaggaaa tcaaatatat caacaatgtg ttgctaaaaa
    11221 aattaaaaat cgccttgtct atgaccactg atgatatttt agacgttttt gctgaggcag
    11281 aaatctatcc atcaaaaggc gaaatcgggg catttttgag aaaagaaggc caacgaaatt
    11341 ttaagccttg cggagataaa tatatgcgta attttctcaa ggggttagga atttataatc
    11401 ggagaaaagt ataaagaaat ttcatgagaa atcaaaacaa tggttgtttt gatgagagct
    11461 agtttttgta tgagattaaa atattatctt aatcgaggag ctggattggt tttatgaatg
    11521 atgaatataa gaatgatgaa gataagatgc tttttgaaga gatagagaat cgatgtaggc
    11581 tcaattttga gcttcgggga aagatgtctt taatacaaca aaaaaagtat ttagcgaata
    11641 aatcagagtt tactctggga catgttgaaa aattaatctc ggattggatt agtagtagaa
    11701 gcgaatttac aaaaataaaa cagccgatta aatttgacat gaaaaaactt ttgttaaata
    11761 agagtgagat aggtaataga gatcaatata ttagagctaa agggcaagaa ataattgatt
    11821 cattaggtga aatgaggtca tacaattatt tgtatgtaac acatagagct gatggaatgg
    11881 tcattactgt ggggaaatct tcttctaatg atattttttt agatggagat cttttttatc
    11941 aactcaatac aaatcacttg tctggcactg aaaatataat tttaaggacg gaatatggca
    12001 atgaaatatt tgcaaaatat gatgagattt taaaaaatta tttggactgg gcttggataa
    12061 ttccggttga gtcaggagat gctaagaaac tggagagatt attgggcgat gaactaatta
    12121 ataaaaaagt accaatttta aattattatt cccatagaca ataaaaggct atttttcatg
    12181 ataatactag ttataactgt aaaaatggaa gaaaatctgg gaaagaagtt tttaaataag
    12241 tgtattgttc gattttaata aataataagc aataccatcc gtctgtgttg cgcaacatcg
    12301 tactacaatg aatatttttt cacaaaaaga aaatcatcca tcgaatgaat ggaaggtttt
    12361 agtatacacg aggttaatgc cccctctttt ataatcagag gagtgaggtt ttttctatga
    12421 atgtttctat tttcttattg ctagtctagt ttctcttacg atgaaattgg gattctattc
    12481 cagtttttat tgatttgctg ataaaataac ttgaatggtg gatataaaca tgataaataa
    12541 gagaagaata acaaaacaag gttgtgaaat gattctcaaa tgaatataat agaaagaaaa
    12601 aggaggaaga atcaatgaaa atagcaattg caggcgctgg cgcaatggga agtcgctttg
    12661 ggttaatgct tcaccaaagt gggaacgaag tattattaat cgatggctgg gcagaacatg
    12721 tccaacaaat caaagagcat ggattacaag caaattttaa tggtaaagag gtagaagcaa
    12781 aactaccaat cgttcttcaa tccgaagtag aaaaagaaga tcaagttgat ctgattattc
    12841 tatttaccaa agcgatgcag ctggaaaaaa tgctgcagga tatccaatca ttaatcaaaa
    12901 aagatacaga ggtcttatgt ctattaaatg gtatcgggca tgaagatatt attgagaaat
    12961 ttgtaccaat ggaaaatatc tatattggaa ataccatgtg gacggcaggt cttgaaggtc
    13021 ctggtcaggt caaattattt ggaagtggtt cagtagaatt acaaaattta ggtgatggaa
    13081 aagaagcagc tgcaaaaaaa ttagcagaca aattgtctga atcaggatta aatgctcatt
    13141 tttctgacaa tattcactat tctatttatc gcaaagcatg tgtgaacgga acaatgaacg
    13201 gactatgcac aatcttagac gttaatatgg ctgagctagg aaaaacatcg actgctcata
    13261 aaatggtggc gacgattgtc aatgagtttg ccaaagtagc agcagtagag aagattgaac
    13321 tagatgtccc agaagtcatt gcacattgtg aatcttgttt tgacccggaa acaattgggt
    13381 tacattatcc ttcaatgtat caagacttga ttaagaacca tcgattaaca gaaatcgatt
    13441 atatcaatgg cgcaatttct agaaaaggga agaaatatgg tgttgcgacg ccttattgtg
    13501 atttcttgac tgagcttgtc catgcaaaag aagatagttt gaacgtaaaa taaaggagag
    13561 attgaatgga aacttcacaa aaaatatcac cgaaaatttt tctgaataaa gtcctcgctg
    13621 gtacagcaac aggaatcatt attggattga ttcctaatgc agtattaggt gccattttaa
    13681 aatattttag tgcgtatccc tttgcggtaa cgatcgcaca gttagcagtt atttttcaac
    13741 tagcaacacc tttgatcatt ggtggtttga ttgccttaca atttggattt gatccgatga
    13801 gaatgatggt tgtagccgga gcttcttttg ttggttctgg tgtagtcaaa ttcacacctg
    13861 atttgggtga aacgggtgtt tacgttggtg ctggtacagg cgatattatt aatacgatgc
    13921 tgacagcatc gattgctgtc gggctgattt tactgattgg aaatagtttt gggtcagtgt
    13981 cgattgtgtt aacaccgatt gttgtaggtg taggtgcagg tttacttggt ttttatttat
    14041 tcccttttgt aacagcaatt acttctgcaa ttggatcatt gattaataac ttcactacct
    14101 tacaaccgct gttaatgtct atcttgattg gttgttcctt tgcctttttg attatctctc
    14161 ctatttcaac agtagcaatc ggaatggcaa tccaactaga tggtgtttct gctggtgcgg
    14221 ctgcgatggg cgtagctgca accacttttg ttttagtagt gaactcttgg aaggttaata
    14281 agtcaggtgt gacaattgcg attgctttag gcgctatgaa gatgatgatg cctaatctat
    14341 ttagaaaacc tgtgattctt attccttgtt tagttagtgc agtgattaca gctattcctg
    14401 tcgctctatt ttctatttca ggtacgcctg cttctgctgg tttcggcgtg gtcggacttg
    14461 ttggtccgtt ggcttcttta gatgcagggc taaatggctt tcttgttgtt ctttgttggt
    14521 tagtcatccc tgtttttgcg gcactaatta ctcaattttt atgcgagaaa gtatttaaac
    14581 tgtatgatcg agaagaagtc ttcaaatttt taggctagta gattttacta agaaattttc
    14641 aatcaatgga tgcgtaagtg agatgtcaac aacatctgac ttacgttttt ttgccttttt
    14701 gagtaggaga ttgatgctga caactgaact cttcagtgcc attcgatttt ttaaaatgta
    14761 gaagaaactc acctttatgt tattgattgt tcaaaaggca actagaataa aaatatgtgt
    14821 taaaatgaga aaaaatttga ttgttggatg tatcgaataa tcactaaaaa gtaggagaag
    14881 aaaatgattt ttttgcaaaa atatggtttt tattttttat tattaggtgt gattagtgat
    14941 ttatcgacac cgtatatcct tggccttttt tatcccaaac tgaatcaaat gactaccgta
    15001 attagtgtgt ttggtgacgt agatagtcca gtcagacgtg ctttcttggt ctggtcagtt
    15061 gtatctggac ttttttttgt tctttcgttg cctgcgttgt atcacctatt tattggaaca
    15121 tcaaaaacac ttgcaatact tgctgttgca acagttggtt tatatgggat aggtgattgt
    15181 atttttactg ggttattcag tatcaatacg aatgaaagct cttggaatct atcgacttgg
    15241 atccataata taggttctgg gctaggctat gcaggctttc tacttttccc attgttactt
    15301 gttctgcttt atcgtcaaag tggtcaaggt acgcttagcc atttttatct tgttttaaca
    15361 gttattagtt tactaattgc aggtctttac ggattagcac ggattccgtc gattagtcag
    15421 tttgccttct ttaagcaatt gggcttcttt caacgcttaa gttttttctt taactatttg
    15481 ccgatgattt gtttaagttt ttttcaacta cgaaagtaga aaaagcagtg tgaagctgat
    15541 taagtaaaag aatttctcta tgggtaacca ttcgtgtgga tgtgttcgac taagaataga
    15601 gatgaggaga gcgaaaagca agttagtctt aacttgttat gcgttgtgct aaatttcagg
    15661 tagtcatttt ttgaatgaat aagaagaaaa cttgaaagag ggtgacttca tgttagatgt
    15721 ggagcagatg gaaatagacc tttataatat tattttaaat cgaaatacaa gagaatggga
    15781 aagaacacaa tttgaagagg caaaagaaga cctagcaaaa atgtcgataa agcaggtgat
    15841 agaaaaacta gaggtcaact tgcgtccgct agcactacga gataatttga caccagatgc
    15901 gatggatttt tatttaagaa tcacagggga gtcagcgagt gcaaaggctt atgattatgc
    15961 caaacataag ataacttcac ccttatacca agaacgggcg atttttgccg gtggctgttt
    16021 ctggtgtatg gttgagcctt ttgaaacaaa accaggtgta atctcagtgt tatctggtta
    16081 tacaggtgga gagaccaaaa aaccaaatta cgatcaagtc agtggtggat atactgggca
    16141 tgttgaagct gttgagatca tatttgatac acgtcaaata acctatgaag agctgttgga
    16201 gatttattgg caaatttcag accctacaga tgcctttggt caatttcaag atcgggggaa
    16261 acaatatcgt tcaattctat ttgttgttaa tgaaaagcaa aggcaattgg ctgaaaaatc
    16321 aagagaacaa ctgattcaat ctaaccgatt tagccgtcct atcgttacag agattcgcca
    16381 agcaaagact ttttggccag ctgaaaatca tcatcaacaa ttttatcaga agcaacctaa
    16441 aagatataaa aagatcaagc attcgcatca gcttcattta ttttttcagc gacatagata
    16501 gaggaaggtt gtggcgcaaa gcgttcagct ccgacaaaat aatgagcaac tgacaaaaat
    16561 agcttttcat atttttggac agttatgaat tatttcgagg agttgctttg agaacaccga
    16621 ggaaaaggtt gtggcgcaaa gcattcagct tcaagtcgca aggaaacagg aggtatgaca
    16681 agtcttttgt cacaactcct gtttttttac tagcctttat ttccatgtga aagagaattc
    16741 gaattgtaga atcttgattt ttctatggaa ttttgtagag aatcatcttg ataattaata
    16801 gacacctaat cgaattccac attaatgatg aactaaattc cgtataaaag cgcaaacaat
    16861 aactata
//
LOCUS       DBNICI010000052        16676 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.052, whole genome shotgun sequence.
ACCESSION   DBNICI010000052 DBNICI010000000
VERSION     DBNICI010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.052"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(184..2892)
                     /locus_tag="AC1ZCC_001374"
     CDS             complement(184..2892)
                     /locus_tag="AC1ZCC_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HIQ1629636.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRRTAIEDGKLTDDEK
                     KNISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQ
                     NRLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDT
                     IGTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGN
                     MDTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEV
                     ETAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQ
                     PVELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQ
                     FVARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAAL
                     FDNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYW
                     PNATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(3198..4145)
                     /locus_tag="AC1ZCC_001375"
     CDS             complement(3198..4145)
                     /locus_tag="AC1ZCC_001375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIQ1629637.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            4335..4604
                     /gene="rpsN"
                     /locus_tag="AC1ZCC_001376"
     CDS             4335..4604
                     /gene="rpsN"
                     /locus_tag="AC1ZCC_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HIQ1629638.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(4780..5142)
                     /locus_tag="AC1ZCC_001377"
     CDS             complement(4780..5142)
                     /locus_tag="AC1ZCC_001377"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIQ1629639.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(5153..6274)
                     /gene="alr"
                     /locus_tag="AC1ZCC_001378"
     CDS             complement(5153..6274)
                     /gene="alr"
                     /locus_tag="AC1ZCC_001378"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIQ1629640.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(6290..6640)
                     /gene="acpS"
                     /locus_tag="AC1ZCC_001379"
     CDS             complement(6290..6640)
                     /gene="acpS"
                     /locus_tag="AC1ZCC_001379"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HIQ1629641.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(6770..8281)
                     /gene="cshA"
                     /locus_tag="AC1ZCC_001380"
     CDS             complement(6770..8281)
                     /gene="cshA"
                     /locus_tag="AC1ZCC_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="degradosome RNA helicase CshA"
                     /protein_id="HIQ1629642.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(8603..9955)
                     /locus_tag="AC1ZCC_001381"
     CDS             complement(8603..9955)
                     /locus_tag="AC1ZCC_001381"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIQ1629643.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(10108..11184)
                     /locus_tag="AC1ZCC_001382"
     CDS             complement(10108..11184)
                     /locus_tag="AC1ZCC_001382"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HIQ1629644.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            11380..13125
                     /locus_tag="AC1ZCC_001383"
     CDS             11380..13125
                     /locus_tag="AC1ZCC_001383"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC1 kinase family protein"
                     /protein_id="HIQ1629645.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(13405..13842)
                     /locus_tag="AC1ZCC_001384"
     CDS             complement(13405..13842)
                     /locus_tag="AC1ZCC_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HIQ1629646.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(13826..15199)
                     /locus_tag="AC1ZCC_001385"
     CDS             complement(13826..15199)
                     /locus_tag="AC1ZCC_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629647.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(15521..16009)
                     /locus_tag="AC1ZCC_001386"
     CDS             complement(15521..16009)
                     /locus_tag="AC1ZCC_001386"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1629648.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(16031..16633)
                     /locus_tag="AC1ZCC_001387"
     CDS             complement(16031..16633)
                     /locus_tag="AC1ZCC_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIQ1629649.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         4672 a         3574 c         3035 g         5395 t
ORIGIN      
        1 ctgtttttat ttggtcgttc ttattacttg atgcagagca gcttttttac aacctcttgg
       61 tacacggtgt tcaatcagct gaccctcggt attagctcaa aaaaacggca aaatatgagg
      121 aactattttt ccattttttt ctctaatact caggtcaaaa cactgatttc acaacctctt
      181 tttttaatga aaaatatggc tctcatgcgc cgcttctttg aatttcgtca ttttttcttc
      241 gatgaacgga tagattgccg ttcctacgat tccaaagaca acaaacaaac cgatcatgat
      301 ccagattgct ttcgtagcat ttggccaata aattcctcct gcagattccc gtaacaagtt
      361 taccgcatga gtaaatggca agaacggatt gatcatttgg aagaatttcc ctgagacttg
      421 gatcggatag ttcccgcctc cgccagagat cgacagtact aagatgataa tagcgatccc
      481 tttaccgaca ttatcgaata atgcagccaa gacatagacg atcatcatga acgctaaggc
      541 gatcaagaca gcaaatgaca cgctgtagac cggattttgg acatctactc ctagtaagaa
      601 aatattccct aatgtaacga tcaagctttg cgcaatcgcc attgtcaaaa atgtcagcat
      661 acgagcaacg aattgttctc gtttcgtaaa attgtttcgg tctttctttc ctaaatagta
      721 ttctgtcgta gcaacacttg aaagaagaac agcgccgacc cacagacata gtgcagtata
      781 aaacggggtg ctagctgaac cgttatttgc gattggataa agcgcattgg tttgtagctc
      841 aacaggttgt gtaaagaagt cactttcttt tgttgcgtct agtttcaata atttgatgac
      901 ttcgcctaga tcgacttctt gttcgccttt tcggatcgcg tctgctgctt tttggatacc
      961 agctttgata tttggccagt cattgttgat caagtccgca gcagcgttca cagctgtttc
     1021 gacttctggc attttttcat ttactgttgt caatgtaccg gtgatttcat tttttagtgc
     1081 tggccaatca ttttggataa aatcagcggc caatcctaat ttttgctgaa tgatcggtaa
     1141 ttcattgttg tataggtctg ccccttcatt gatgccattg acgatggtat ccatatttcc
     1201 attcagcatt gtattggcat catggatttc ttgtccaatt gcaggcattt ctttttgata
     1261 tttttctaaa attgccactg cattcgttac agttgctttc gttgagctga gaagcgaatc
     1321 aaagtcgatc tggtccgctt tgctcagtgc gccttgtgca gtcccaatcg tatcgatgac
     1381 ttggtctagt agagaagtga cagtggaact gatttgacct gtattgatac cagcaatgat
     1441 gccattgata ttattaacaa cggcatcaat ttgatctagc aattcatcaa tagccttcgt
     1501 gtcttcattt tgaacagctg tgttcaaagc agataggcgg ttttgcagat tgcttagcaa
     1561 gggttttaat tgatttagct gatcaataat tggctgtaat ttcgtatttc cagcacttgt
     1621 ttgcaattta gtcaggaact cgaccagttt atcgattgca ttttgttgtt gagatattcc
     1681 gtcaataaag ccgttcaata cattgctgat gtttttcttt tcatcatcag tcagcttacc
     1741 atcttcaata gctgttcttc ttacagtatc tattaacgaa cctgtcgttg ttgccacttg
     1801 tccaattgac ttcaatgtaa catctacact gtttgtgatg cttggtatcg catctttcag
     1861 ctgagaggca cctgattttg tttggtttgc tagatcactt gcctgatttt ctaaattttt
     1921 cacatctggc aagatatctt gtacttgttg gatgatcgtc aagccttgtt ttgcttcatc
     1981 gattccttgg ttcattgtct cttggatcga agcaaaatcg ccgtcgatct ctgctagttg
     2041 cttaccggca ttttggatct ctgggatttt ttcttgcaaa tcaaggatga tttttgcttg
     2101 atctttcaac tcaggcattt tgtcattcaa tgcaacgact ttttcaccca tctcatctac
     2161 tttagggatg taatccacga attcatttgc tttattcagt ttttccttga tttcaggtag
     2221 ttgactttgt aattcgagga cttgttttgt gtagccgtcg atcgtgtcca aattttcatc
     2281 tgttgataaa atcatatctt taactttatt gatacttact aggttcgtat cgatgtcgta
     2341 acctatctca ttgaatactt tcaataaagt cgaactggct gttttgataa attcatttgt
     2401 gatttgcgat tggatcgaac ttgctccttt atcggtgatt ttcggtgcaa tcgcattgat
     2461 tttttcattt accgtatatt cgatttttgg tttcttgata tcaccactag taaaactcag
     2521 cagatcttct gagaaatcct ttggcaagta aatacctgcg taatactttc cggaacggac
     2581 tccgtcttct aattgttctt ttgaatcaac gaattgccat cctaattgtt tatttttatg
     2641 aagcgattcg atgacttgct ccccaatggc cacttcttta ccttgaaact ctgctggctt
     2701 gtctgcactg taaacagcaa ttggcagctc tccagtattt ccatatggat cccataatgc
     2761 tttgatgtta aaccacgcat atagcgaagg gatgatcatg atcgcgatga tcagaagtgt
     2821 tgcaatcggg tttttgaaaa tccgtttcca gtcaagcaaa tataatcgaa gcgtattttt
     2881 gattgactgc atagttctcc cccttcttct tttttcagct gaattacttt accatgtatt
     2941 tcatactttt ttcaatcggc ttttttcaaa aaagtcgaat ttttggacat atcacggcaa
     3001 ttgtctcatt gtaatctctt acatattgag aaataattaa ggattacagc ctttacttta
     3061 tcatgaaata aaagggaaag ataattttat gccaaagttt ttatctatta tacgtatacg
     3121 caaaaaagct aaaacatttt ttgtctttgc ccctacaaaa atgtttcagc ttttttcgtt
     3181 ttttattcct gtatttctta tttttcgaat gacaggatga ttttcccagc aggaggatgt
     3241 ccttcaacta gctgatgtgc ttggatgaca tgcttaagtg aagcagacag attcattgaa
     3301 ataaaaacat gaaaggctcc tttttggtag gctttgatta tgccagcaaa ggcttctgca
     3361 tctagatatt cctttcttgg aacaaacgat tcataaaagc cttccttttt ttgccgcaaa
     3421 tctagatctg ggaggtcatt taatgcaaca tacctgccgc ctggtttcat gatctggatg
     3481 ccggtttctc ctttgatact tcctttcgtc gcatcgatta caaggtctgc ctgatcagca
     3541 aattgcagac ctggatttgt tttatcataa gcagcaaatg ctgaagctcc taattttttt
     3601 accttttcct catttttact ggatgcactg gttaagattc ggattccctt ttcatggaga
     3661 agctggatca gacttgaccc gaccgcacct gatgctccta gtatcattac agtatctgtc
     3721 ggctggattt ccgtcaaatg gttgattaga ttataagccg taattcctgg cgtgaccatt
     3781 ccggctgctt cttcccaact cattttatct ggaatttttg ctacttttgc tgatggcaac
     3841 acgacttctt ctccgtaagt cccgccgact gcatggacag ctactcgatc acctacatga
     3901 acagttgtga catctgaacc aatctctgtc acaataccag cgccatcatt acctggtaca
     3961 taagggaatt tcaatgttcg tatctctttc atttctccta aacgcaaggc aacatcataa
     4021 ggattcacac tgaatgcttt tatttctaca cgaacatgtc cagaaccgac ttctctggga
     4081 tgagcatcga tttcttcaaa aacatcttgt gcttctccgt aaccgttaat tgcaaatctt
     4141 cgcatggtca ccatctccct ttttctttaa gtttacgttt atgcctttat tttgacaaaa
     4201 aaaatgtctt ttcgttttgg cgcatgattc aaaacaaaat agttgtcttt tttcgcgtaa
     4261 cgtgttaaat tatttatcta aaagcgtaaa gattacgatt tacgctaata aaaaataatt
     4321 ggaggaattt gttgatggct aaaaaatcaa agattgccaa agcaaaaaaa cagcaagaaa
     4381 cgattgaacg ctatgctgct ttacgccagg aattaaaagc gcaaaaagat tacgaaggct
     4441 tgcgtaaact tcctttagat gctcgtccag aacgattgaa aaatcgtgat ttaatcgatg
     4501 gacgccctcg cggatacatg cgtaaattcg ggatgtcccg tataaaattc cgtgaattag
     4561 cacataaagg tttgattcca ggagtaaaaa aagccagctg gtaatccaag tctggctgta
     4621 atgtaaagga ggagggcgca tggaccactc cttggatcat caaaaaagat acgttcgctt
     4681 cttctagcag aaatgctggt actagaagca gacgtatctt tttttacgca cagctggtcc
     4741 ttttttatat gtaaaggctg catggttact tcgctatatt tatttagtaa caggagtcag
     4801 tgcttcgata cccacactaa ttttcaatgc tcggtcaact tctgccataa tgggtcgatc
     4861 caaatggcag accttttctt tcaatcttga tttatcgatc gttctgatct gctccaacag
     4921 gataacggag tctctttcta ttcctgttcc agtagctttg atcccaatgt gagtcggaag
     4981 cttaggttta gccatttttg ccgttatggc cgcaacaatg atcgtcgggc taaaatggtt
     5041 acctagatta ttttgaataa ccaaaactgg gcgtgttccg ccttgctccg atcccacgac
     5101 tggagaaaga tcagcaaaat aaatatctcc tctttttacc ataggatcct ccttattctt
     5161 gatattctct tggtatccgc tgtcctaatc cgcaagccac ttcatagtgg atcgtaccta
     5221 attgatctgc tatatcctgc atagtgattt ctttgtctgc atttttgccg ataagcgtca
     5281 cttttgttcc gacaggaaat tcttgaggca atcggatcat cagttgatcc atacatacac
     5341 gcccaatcgt ttcacagtag ttcccttcta ccaatagaga aaaaccctgc atctttcgtg
     5401 gccagccatc tgcataaccg atcggtatcg ttccgatcca ctctgcttca ggagtgatat
     5461 atgtttctcc atagccgatt ccttcacctg ctgggagttt tttgacttga atcaattcag
     5521 aaaccagctc aagtgctggc tgcaatggat aaacttccgg tagtgcgtgc ccagagggat
     5581 taagtccgta cattgctact ccataacgaa tcatattgcc gattgtttca tcatgccata
     5641 aagccgttgc actattgctg acatgtacat atcgaggaag ttcgggaagt tttttcagca
     5701 cttctataaa gcgttctttt tgcagattcc aatatgtatc atctgcctga tctgctgtag
     5761 aaaaatgggt aaataaacct tcccattcta atacacgact ttctttgatg aatgcagctg
     5821 cctcttttac ttcttcaggt gtacaaaatc cgatacgacc catcccagtg tctgctttta
     5881 gatgaatcga aagaggcgtc tctagcttac ttttctctaa aacagcagct gctgcttgca
     5941 accattcccg tgtacctgca gttactgata aatcatgagc aacagctaca gtcagtgcgt
     6001 cgtaaggaac catattcaag ataaggatgg gctgagtaaa tccagcttct ctcaactcga
     6061 ctccttcatc taaatttgaa acacaaaagc ccgaagctcc acctgcaact gctgcctcag
     6121 ctgtttcaat agctccatga ccgtatccgt tcgcttttac tactgcaaac aattcttttc
     6181 cttgcggaag tcttgcaact tcatttgcta cattttcagt tatagctttt ttatgtatca
     6241 ctgctttcgt tgggcggtgc caagcgacaa ccatcgtttt tccttctttc tagtcttcca
     6301 aaataatttg tgcaaaagct gtttcatttg tatgagtaat actgacccat acattgcctt
     6361 catgaggtga acgcgtcaca acaggctgac cattttcatt ttttaaaatc tcgacatctt
     6421 gaaaggtaac ttttccgatc ccagttcccc aagctttaga gaaagcctct ttgcaagcgt
     6481 aacgcccgcc taaaaattct acttggcgat gaaaaggcaa ggactggaat agtttcattt
     6541 catcagaggt caagattcgt gcaatgaatt ttggtttttc ttcgatcagc cgagtgatcc
     6601 taggaagttc gactgcatcg atcccaattc cttttatcat atgcttttct cttttcaata
     6661 aatttagaac actctcattc taccaaatta taaccatttt gccacaaata agtccacaaa
     6721 taacaaaaat gaaagtttct cttaaaaaag agaaactttc attttatgtt taatcgttat
     6781 ttgaacggat tacaaatccg cgttttttat cattgtgacg tttaccgcca tctttttggc
     6841 tgttattatt tttgctataa ccgccttttg atttgtttcc acgataatta ccgccgtcac
     6901 gacgattacg gttgtttccg ccaccacgtt tgccattttt attgtatcct tttttctgca
     6961 ttggcaatgg gcgttctggt gtgatcttca ctggcactgc atcagctgga tcttttgctg
     7021 tcgtttttag caaaagtgca actaaatctt gtgcagaata ttcttctaat aatttatctg
     7081 ctgtttgcag gtatttatct agaccatttt ctgcaagttt tgtttcgatt tgttcaactg
     7141 cagcacctaa ttgacctttg aatgcttctt tttcggttgg tggacgtaaa gtcgtcatcc
     7201 gttttttcgt caagttttca atcacatgca agtagtccat ttcatttggt gtaacgaaag
     7261 tcacagacat accgcctttt ccggcacggc ctgtacgtcc gatacggtga acgtagcttt
     7321 ctggatcttg agggatatca tagttgtaca catgagtgac acctgaaata tctaatccac
     7381 gtgcagcaac gtctgtcgct acaaggatat ctaaatgacc gctcttaaat gaacgcaata
     7441 cgctcattcg tttttgttga gacaaatcgc catggattcc ttctgcacgg taaccgcgtg
     7501 cttctaatcc gcgtgccaat tcgtctacac ggcgttttgt acgtccgaag acgattgtta
     7561 attcaggtgt ttgaacatca aacaagcgag tcatgatatc gaatttttcg tattcttttg
     7621 cgcggacata gtattgatcg atcaagtcag ctgtcatttc tttcgctttg atcttcacat
     7681 gatgtggatt tttcatgaat ttaacgccaa tattcttgat agctggcggc atcgttgctg
     7741 agaaaagtaa tgtttgtctt tgatcaggaa cttgtgaaat gattttttcg atatcttcca
     7801 agaagcccat attcaacatt tcatctgctt catccaaaac taatgtttga acggttccaa
     7861 gtttcaatgt gtggcggtta atgtggtcaa gcatacgtcc tggtgtaccc acaacgatat
     7921 gtggacgatc tttcaagcca cggatctgac ggccaatatc tgctccgccg taaacggctt
     7981 gtacacgtac ttttttgtcg cgccctaaac gatataattc ttcttgtgtt tggatagcta
     8041 attcacgtgt tggtgcgata actaaacctt gtaattcgtg acgatctgga tcgatttttt
     8101 ctaacattgg taatccaaat gcagctgttt tccctgtacc tgtctgtgct tgtccaataa
     8161 cgtcttttcc agctaatgct agtggaatcg tttctgcttg gataggcgtt gcttcttcaa
     8221 aaccagcgcg ttcgactgat ttcaacaatt ctggtgataa ttctagttct ttaaatttca
     8281 aatgaatcct cctatatggt tgttaactga ggatgtagct tttttgctac cccgtcgatt
     8341 tgttcggtca aagtgtattg tttctttttg acagtggcca cgcacgtttt tatccattct
     8401 tagcccctgt ggcaccaccg aaaaatcact ttgcgaagtt taggcattag aaaagccaaa
     8461 cgggtcgcgt tcagctttat cattattatg acatctggct tcaacaagtt gtttctatgg
     8521 caaaaccagt ccaatttctc ttcatctttc ggcaataggt tattctaaca tagtttttcg
     8581 ttttctgcaa gtttacagat ttttatttca tttgctgaag ttgttctacc acttcaaata
     8641 agcccatgct gttgctgcct tttagcataa tactatctgt tgcttcaaga ttttgtttga
     8701 tagcatcaat caatttattc ttttcttttt ccaaatagaa cacaggtagt tgggggtatt
     8761 tttggttcaa ttcttctttt agtgcatgca tttgttcccc ataaaggaaa atcatggcga
     8821 atttctcagg atcgatatgt tcactcattt gcgcatgcat atttaaagac tctttcccta
     8881 attccagcat atctgctaac acagcgattc tgcgtccttt tgttggcaga tttttaaaag
     8941 tatctaatac cagtcccatg gctgttggat ttgcattgta gacatcgctc aaaatagctg
     9001 caccatttcc tgccgttagc cattcggttc tgttttgagt gatagaaaca tgggcgagcc
     9061 cttggcgtat ctcaggtatg gacagtccaa agaagcgccc gatcgtatac gcaatcaaag
     9121 cattcgttac attataactt ccaggcagcg ggatcgtaaa tgtttctcct tctactacaa
     9181 aatcggtttg tgcttttcct tttgctgtca cttccgcatg aacatcgcca tttccaatac
     9241 cgaatgttgt gactgtttgt actaatgttg ccgttaaagg tgtcagcaac ggttcatctg
     9301 cgggcacaac gagtaatccg tctgatacaa gtccgtcagt gatttccatt ttggctttag
     9361 cgattccttc tcgcgaccca agattttcta tgtgtgcttc accgatcatt gtgattgctg
     9421 cagcttttgg ttgagccata agtgaaagtt ccgtgatttc attggcgtga tccatgccca
     9481 tttccaaaac gatcttttct gtatcttggg gcatgtgcaa aatcgtgtaa ggaagaccaa
     9541 tattattgtt gtaatttcct tgcgtcttat atgttttgta tttttcagac aagacagacg
     9601 cggttagatc tttcgtggtg gttttaccat tgcttcctgt aattgcaacg acttctgcgc
     9661 ctactttttc caagtaatag gttgccaatt gctgcatggc tttcaaagga tctgctacag
     9721 gaataactgg aaaatttttt ggagcttgtt caacaggcaa gctccaaaat gaagcagatg
     9781 ctccgtttgt gatcgcttgc tggataaatt catgtccatc tcgcgtacca gcaagaggaa
     9841 cgaataaact tccttcggtt atcttccggc tgtcaaattc gacattggta atctttgtca
     9901 gtgcagagca ttcgctgccc acggcttgtg caatttcttg tattgttaga ttcattgaga
     9961 tctctcctaa aaaaggttgt gaaaaaagcg tttggcctaa acggcgggtt cgcatgactc
    10021 tcacttccaa gtaaatttct ctattcgtat ttagaagcta gaatgaacgc ggttacgcaa
    10081 tcactccagc ctcttcttta tttctcttta ttcatttttt tcaaaaaaag attgacgctg
    10141 atggtatcga ttcattccta actggatcag ttcttcaatc aaatcaccgt atttcaagcc
    10201 catattttcc cataagagtg ggtacatact gaactccgta aatcctggca tagagtttaa
    10261 ttcattcagg aataattcat ttttatttgt caaaaagaaa tcgcaccggc tcaatccgct
    10321 tccacctaac atcgtgtaag ctaacttcgc gtactcttgc gctttttgat aaacttcttc
    10381 tggcacttcg gctggaatct gcatttcgat tttattattg atatattttg cttcataatc
    10441 atagaatgct acgtctttta cgacttcacc aggcaaagtc gtccgaacgt cttcatttcc
    10501 taatacagca acttcgattt cgcgcgcttc aattccttgt tcaacgattg ctcgagaatc
    10561 atactgatag gctgttgcta aagcattttg cagctcttct cggttttctg cttttgtaat
    10621 gccgacacta gaacccatat tcgccggttt gacaaacatc ggataaagca aagaaccttc
    10681 acattgatca aatacttttt taggattttc tttccattga ttcttaagta ctggtacata
    10741 aggaacttgc ggcacaccag cagcttgtaa aatatacttg gtcatgattt tatccatggc
    10801 acatgcactg gtcaatacgc ctgcgccgac ataaggcata ttcaatgtct ctaagaagcc
    10861 ttggatcgtt ccatcttccc cgtttggccc atgtaaaact ggaaaaacga tggctccttc
    10921 ttctttgatt tcgcccggat tgatcacttt tcctgtaaag ccttcctctg tctgtccact
    10981 tgggtcccat gaaagatgca agacatcttt gctggcaggt ttttctgtta gtaatggacc
    11041 tttgacccat tgtccttctt ttgtaataaa tacgagttga acttggtaat aattataata
    11101 aatggcattt aaaactgaaa atgcggaaag aatggacact tcatgctctg cgctgcgtcc
    11161 gccatatagt aaagtaatct tcaaaagatt gtcctcctgt attctaaatc gttcagtgat
    11221 ttactctcat attttagcac aaaacaaaaa taaacgaata acttatttat aaaaaaacga
    11281 gccagcttct aaaagaacga gcgtcattta ttttttgtca gctagattat ttgtcccgtt
    11341 ttttataaaa agttatgata aagaaaagga gggatgatga tggcgcaaac aaatccacgc
    11401 agccgcttgg cagaaatcat tcgagtgttc atcaattaca atgtagtgcc aaactttgta
    11461 cagcaaaaaa atcccgaaca ggtaaaaaaa gcctttgaag aactaggccc caccttcatc
    11521 aagataggac agatgctttc tgttcgagaa gatttgcttt cttctgcctt cactcaaaca
    11581 ttcaaaacat tacaagatag tgtacctagc gacacatttt caacggtcaa aaagacgatc
    11641 gaaacagaac tttctctttc attatcagat atattcgatg atttttcaaa atcgcctttt
    11701 gcctctgctt ctatggggca ggctcatcgt gccaagctga agaacggtga cgcggtagtc
    11761 gttaagatcc aacaccctaa catcgccgaa gagatccgac ttgatcttca actatttgaa
    11821 cgagcgatcc ctttgatcaa atacattcct gaaactagcg tagttgatct aaaaggggta
    11881 ctccaagaag taaaacgttc cttgatcaat gaaatggact tcttaaaaga aagtcaaaat
    11941 ggcgaacaat tctatcaaaa gaacaatggc tggaaagaga tccgcagccc caagatttat
    12001 gacgcttttt gttcgaaaaa agtgatcgtg atggaagaaa tgtctggtaa aaacttgaat
    12061 catttaatga acgccgaaaa caaaactgaa acttttatca ctggaattca aaacaagcag
    12121 ctgaaacaag aagttgcaaa gcttttagtt gagaacttta tgaaacaggt gtttgatgat
    12181 ggttttttcc atgctgatcc tcatccaggg aatctgctat tccatgtact aacaaaagaa
    12241 gaacagacac aagctagccg aaaaactgaa acagtccatg aaaaagagtt cggttccttt
    12301 gcttttcgag cctccacttc tgcagaagac cctgttgcgc catataccat caactacatc
    12361 gattttggta tgatggggca tctttctgca ggacttaggc aaaaactcac acaagccgtt
    12421 ttagcgctgt atacgaaaga tgcttatcga atcgaaaaag cggtccttcg actttgtcag
    12481 caagaaggct cgtttgatga aagtcggttc caccaagaac tgacaagttt tcttgaacag
    12541 tattatgatt ctcctattga tgaaatcaac ttgcaagaag tattcacaca tgtcgtgacg
    12601 atttgtcatc aaaataatct gcagttcgat cgtgatatca cgcttttgct gaaagcattt
    12661 gggacattag aaggggtgat ccgcgtcctc gatcctgaag tttccctcat ggaagtcgct
    12721 tcgccttttg ctcaacatta ttttctcact catctggatg tagaagatac attgaaacaa
    12781 agcggattag acttattgga agggatgaag gctgcgccta agatccctca acagctccac
    12841 catctattag aaatgtggac ttcgggacaa ggaaaagtca atttggaatt gaaaaagcaa
    12901 gacaaattat tgagcaggat cgaaagtatg atcaatcggc ttgttttcgg tatgatttta
    12961 gctgcattga tcgtagggtc ctccctgctt gtccaagcag cacctgtaga aaacgcagag
    13021 gtcgtttcct tattagggat atttacgtat gctattgccg cattcgtcat tatttttttg
    13081 gcaatcgatg cattgatcca aatgtataag aagaggaaaa agtagaaaaa ggttgtgaca
    13141 gaaagaatca gcttcaagaa ataagaaggg gttttacata aattgtttct caaatttttg
    13201 tgaaaattcg gcttattttt cgaagaggct acttctgaaa caccgagaat aaaaaatgtg
    13261 aaaaagctgt tctgcaccca gtaataagaa caatcaaaaa gggaacatgg aacacattta
    13321 tcctgtttcc ctgttccctt tttgatgtat tcgagcataa ataaaatacc ttcgttacct
    13381 actatcttct gtacttggta aaccttagtc aattgccttt tttaacgctt gacgaaaaga
    13441 catatgatct ttctccgtaa agggttttgg accctttgtc cttatgccag cttttcttaa
    13501 ctttccacta tgataacgtt gttctagaag cgtgccgata ttcgcttcgt caaattcttt
    13561 tccagagtga tggaacactc gcacaccctt cggcaataca agtgaagcta aaccataatc
    13621 ttgcgtgatc aaaaagtcat tcttcttaac cataccgacg attttataat ccgcactatc
    13681 agctccgcgg tctacgtaga cgatttggac atgattagcg tattctttgg tcgtataatg
    13741 gtccacactg gtcacgataa ccacgtcaag accataggat tctcctaatt ttatgacatc
    13801 ttcttttact ggagaaccat ctccatcaat gaatagcctc attctttttc gccttcttcc
    13861 ataaatgata aacagttact aatccgctca gtaaaagacc aacattgatc cattttgcca
    13921 agataagcag ttgtacactt tctatcggca ctgattcatg gttcagcgtg atcaataata
    13981 gatggattgg ctctcccatt tcttttgcta aaacaaacaa aagcgtcatc aataaaaact
    14041 ggtggatcac actcccttta ttttgagtag tatacttgac aacgatccgg cgatccattg
    14101 gaatagctgt atcagtttga taatattcct tatttagcca gctgcctgtt actacgtgcc
    14161 agaaactaag cagaaagaca cctatcgcaa aaccatttgg aatcaagagg accaataaag
    14221 aagaactcag tccaagcaat tgcatagcca atggagacca tgttaaacgt ttcaaaatcc
    14281 atggtccgac tatttttgct gcaatcaagc ctgctgcata aggcaaatac ataaaaatcg
    14341 aaagtttttc taatccgtac agtatgccaa cataaaaact gctaaataaa aaaaggaaat
    14401 tccctagcat tccatttaga aaagtaaaca gatttagcca gcttggcaac ttccattcct
    14461 tcttctggtg tcctaaaaca aacacgaaca agatgaacag cagcaagaaa ccataaatag
    14521 caagatcaaa aagcctttca tcgaataaca atcgggcatt tctcaaaaga aacaataagc
    14581 caaagaatat caaaaataaa acaagctctc gcgtagctac cagatttctt ttgatgtctt
    14641 tgaaatccag ttcgtaacgc ggataatgac taaccgtatg gtaggccatg acgtaaaaaa
    14701 gtgtataaat agtaaagctt gctgcaatct gagtcggtgc aggcaactca atcgcttgat
    14761 acaggagaaa tagccagata gctgcaaata agtacttttt cccatccata ttgataaaac
    14821 cttgcgtctt ttcgtgaaaa ttgaccgtgg tattagcagg tacaagccat gctgcactca
    14881 gccctagcaa aacacttgaa aaataaaata aaggaaaata aaatacgcct aatattccaa
    14941 gcaatgaacc agcaccacca atcagcagag cgatcatcag caacgtgtaa ctatctagac
    15001 caaatcgtag actgttgact aaaaatagtc cagtcatccg aaaggcataa aacagcgtaa
    15061 atggcaaaat cgtcagccaa ttatgcgagt ctgttagtcc caaaaataaa agataaggaa
    15121 taaaaataac cgtgttactt aagataaagg gcgttgtttg tgtaaaatta cgcccaaatc
    15181 gtttgagttt ttcgaacatg acaatggctc cttaaacagt cttataacta atctgctctt
    15241 cgattttagc attaaagggg aaataaacca attttacaga atatattctt gtaaaaaaaa
    15301 atacagaaag aagcataagc ttctttctgc atttttacgg tacttgatta ctcaagcaga
    15361 caagcccttc tccacaaaag tcttgcccga cacccctgca tccggcaggt gataaagata
    15421 gaaatcaatt atcctgtgcc tccaaaatca attaggagct ctgtaaacca gagtccttct
    15481 cgacccgtcg cataaattta ctttaacaca gttcctatgt ttatgcgaga gcttttttca
    15541 ttttataatc gaccatttga aatccttgac cgatatctga aataagttct tctgtaatct
    15601 caaatccttc atgcttataa acctcgattg cttgatgatt cccctgattc actcgcaaaa
    15661 agatctctct ttcctttgtt tgcgcagcaa gttcttcata ccagcgaaaa actgaagaag
    15721 tcaatccttg accacgtact ttatttagca aatataattt acttaaataa atttttccat
    15781 ttatctcttc ataagcagtg taaccgacag tcttttcttc gctttttaat aaaaagtagg
    15841 acacaccttc tgtcagttct ttttgaatct gtttttttga ctgataagtt ttcagcatgt
    15901 aatccacttg tttttgacca atgatcggca caaaagcttc cggccatact tcttgtatga
    15961 ctgccatcag ctcatccagt tcattttctg ttacttttat tttttccata cacccacgtc
    16021 cttttttaca ttataaatga ccttgcgctt gatggaataa aaagagcatg atcatgctat
    16081 acacacccac cgaccatggt ttcaagaaaa tcacaatctg cataaattcc ctaaaagata
    16141 atttcgtcaa accagctact agacagatca agtcgtctgg cgcaaaggga agcagcaagg
    16201 taacaactag tactttttta atatctttcg tttttcttgt cagcaattca tcaaatttct
    16261 gatacttttt tggcgataaa ataagctgga caaaggctac gccatagtaa cggacaagca
    16321 aaaatccgat tgcttcccca atcactaggc caatacaact atataaaagc ccaaatccat
    16381 tgccaaacat cagcatgccc gctactgagg agatccctcc cggcaaaata ggaatgatca
    16441 cttggatcac ttgcaacaga acaaatatca tcactgcata cttgccaaat tgctggataa
    16501 acgcttgcaa agacgtgctc gatcgaaaaa tcccatgatg ataagcataa agaatcaaca
    16561 caaagaaaaa aaacagacca ataatgggca tgatctgaag acatttttgt aaaactgatc
    16621 cttttttaga catctatatt ccccacatct tttttcattt attaaaactg cctcct
//
LOCUS       DBNICI010000053        16594 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.053, whole genome shotgun sequence.
ACCESSION   DBNICI010000053 DBNICI010000000
VERSION     DBNICI010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.053"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            50..1189
                     /locus_tag="AC1ZCC_001388"
     CDS             50..1189
                     /locus_tag="AC1ZCC_001388"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HIQ1629650.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            1182..1982
                     /locus_tag="AC1ZCC_001389"
     CDS             1182..1982
                     /locus_tag="AC1ZCC_001389"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIQ1629651.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            1982..2809
                     /locus_tag="AC1ZCC_001390"
     CDS             1982..2809
                     /locus_tag="AC1ZCC_001390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIQ1629652.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            2814..3521
                     /locus_tag="AC1ZCC_001391"
     CDS             2814..3521
                     /locus_tag="AC1ZCC_001391"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIQ1629653.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            3621..4487
                     /gene="rfbA"
                     /locus_tag="AC1ZCC_001392"
     CDS             3621..4487
                     /gene="rfbA"
                     /locus_tag="AC1ZCC_001392"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HIQ1629654.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            4501..5073
                     /gene="rfbC"
                     /locus_tag="AC1ZCC_001393"
     CDS             4501..5073
                     /gene="rfbC"
                     /locus_tag="AC1ZCC_001393"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HIQ1629655.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            5095..6123
                     /gene="rfbB"
                     /locus_tag="AC1ZCC_001394"
     CDS             5095..6123
                     /gene="rfbB"
                     /locus_tag="AC1ZCC_001394"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HIQ1629656.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            6221..7072
                     /gene="rfbD"
                     /locus_tag="AC1ZCC_001395"
     CDS             6221..7072
                     /gene="rfbD"
                     /locus_tag="AC1ZCC_001395"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HIQ1629657.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            7106..9139
                     /locus_tag="AC1ZCC_001396"
     CDS             7106..9139
                     /locus_tag="AC1ZCC_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7657 domain-containing protein"
                     /protein_id="HIQ1629658.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            9183..10463
                     /locus_tag="AC1ZCC_001397"
     CDS             9183..10463
                     /locus_tag="AC1ZCC_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HIQ1629659.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            10673..11479
                     /locus_tag="AC1ZCC_001398"
     CDS             10673..11479
                     /locus_tag="AC1ZCC_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1629660.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            11491..12711
                     /locus_tag="AC1ZCC_001399"
     CDS             11491..12711
                     /locus_tag="AC1ZCC_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1629661.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            12701..14287
                     /locus_tag="AC1ZCC_001400"
     CDS             12701..14287
                     /locus_tag="AC1ZCC_001400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIQ1629662.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            14326..16464
                     /locus_tag="AC1ZCC_001401"
     CDS             14326..16464
                     /locus_tag="AC1ZCC_001401"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIQ1629663.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         5269 a         2750 c         3545 g         5030 t
ORIGIN      
        1 catatataat ttataataat tatgactctt aattaaagta ggtgcccata tgcttgtttc
       61 ttttagtatg gttataagat tttttctgac aattttatta tcactgatat taacacctgt
      121 attcaaaatc atttcagtac aaacaggtat ggtcgacaaa ccaaatgaaa gaagaataaa
      181 taaagttcca atgccttcag cgggcggact gcctatcttt atttcatttg ttatatcgac
      241 tttattctta ttccgtaaaa ttatccctca atcgtatatt gtaccgatct tgattgcttc
      301 tttagtaatc atagtcacag gagtgctcga tgataaatat gagctgacac ctaaacaaaa
      361 aagtataggg atcttgattg cttctcttat tatttatttt gtagcagata tacgtattga
      421 ttccttcacg cttcctttta ttggatatat ccaactaggc tggcttagtt ttcccgtaac
      481 gattttttgg atcttcggta tcacgaatgc ggtcaatctg attgacggtt tggatgggct
      541 ggcggcaggg atttcattga tcggtttaat tacgatagga atcattggat attttttcct
      601 ccatgcttct acagtgtata ttccagttgt tattttctgt cttgtagcaa gtattatcgg
      661 tttttttcct tacaattttt atccggcaaa gatttattta ggcgatacag gagctctatt
      721 cctagggttt atgatggccg tcctttcact acaagggctg aagaacgtga cttttgtttc
      781 atcgatttct ttgctgatcg tcatgggagt gccggtaaca gacacgtttt ttgctatcat
      841 taggagaaaa gcaaaccgtg tttctttttc tacagcagat aaaaaacatc ttcatcaccg
      901 actaatcagt ttaggcttta ctcataaagg tgctgtcctg acgatttatg cgatggcttt
      961 gatgttttct ttcacagcta tggtcatgaa ttatactgga agaatcggaa cgatcatatt
     1021 gattattgcc atgttgtttg ctgcaatttt attatttgaa ttgattggtc tgatcaatga
     1081 gaaacatcag tttattttaa aaacattgcg atttttaggg aataaagagt accgtacgca
     1141 agtaaaacag aaatatttga agaaatggaa ccgtcatata gatgaataac aaattaaacc
     1201 agccgatcct tattagtatt gtgacggcaa acagtgataa aatttttcgg acactagata
     1261 attttatttc aactgtaaaa gatgaaacag cagtaaaaat ccgaattttt gacaacaatt
     1321 cacttcccga gtacaaaaaa agactaacag agtataggaa atatccctac atcgatatct
     1381 attttcatga tgaaaattcc ggatttggtt atggacataa tcacaatctg atagctagcg
     1441 attctcgcta tggcattatt tgtaacccag atattttagt ggatgaagag acagttacag
     1501 cattggtctt gttattgaaa gaacatccgg aatgtgcgat gttagcacct aagattctta
     1561 atgaagatgg aacaactcag cacctcatcc gtgagcagct ggctgttttt gattacgttt
     1621 tgcgctttgt gcctttcaaa tgggtcaaaa aaaagtttga caaacgactg gctgtgtttg
     1681 aatgccgagg attgcctact gatgctaata gttatgtacg gatgatctca ggtagtttta
     1741 tggtagctga tattgataaa tttaaagaga taaaggggtt tgatgagcgt ttcttcatgt
     1801 actttgaaga caacgacttg tgtttgagtt ttgaaaaagc aggttacaag ttattgtaca
     1861 caccctttta tgcagttatc catttttacg ggaagggtgc gcatcgaaat gcaaaactgt
     1921 ttcgcgtgtt tttgacttct atgatgaaat ttttcaataa gtgggggtgg cgttttttct
     1981 gatgaaacca gaaatattag tagtcgttgt gctttatcag caactattca gtcaatctcc
     2041 agcttatgga ccattaagca aagcacttag cgaaaagcaa attcagctta ttatttacga
     2101 taatagtcct caaaggcagc aaaatgagct gtttgaaaaa gaaaatatcg tttatcatca
     2161 tgatccgcaa aatcctggtt tagcggtggc ttataatttt gcgttgtctc aagcagatga
     2221 aggaacacgt tgtctcgtca ctctggatca ggatacgagg ctagtcgacg attattttga
     2281 actattgcgt aaagtaacct ttacagatga atgtgttgct gcagttccga tgattttttc
     2341 tgggagccga caagtttctc ccctatattc agatcgttat atcaatcgta cagctcaagc
     2401 agtagaagtt gaaggcccaa cagctgaacg aatcatggca atcaattcag gtgttgcctg
     2461 gtctgtcaaa tttctaaaaa agattggcgg gtttaatcct gctttttctt tagatttttt
     2521 ggatcattgg ctgttttgga aagtggatca gttggaaaaa actgtggaga tccttcctgt
     2581 acgaatggaa catgatcttt cggttttaga ctatgaaaaa gtttcaagta atcgataccg
     2641 ttctatttta caagcagaaa atcgatttta tcatgaatat gatcaaaggc atttatccag
     2701 tcaccgacga caattattgt taagaacagc taaacagttc ttgaccgtca aaaaccggca
     2761 tatctggcgg atgactttgc gctcatatgt taagaattgg aaggtgtgaa ttcgtgcgtt
     2821 cggtttgtat agctaccttt aatggaggca gatatttaag agagcagtta gatagtattt
     2881 tatctcagct ttcagcagaa gatgaagtag tcatttctga tgatgggtca gtagatgata
     2941 catgggatat attaactgaa tacgcagaaa aagataagcg aatccagctt ttttcggggc
     3001 ctcgtcaagg attgatcgct aattttggtt atgcgatcca gcagacgaaa ggagatctaa
     3061 tctttttggc agaccaagat gatgtttggt tggagacaaa agtgaaaaag atcactgact
     3121 attttgagca gaatccggat cagctagtag tagtttctga tttaatcatt gtggacagcg
     3181 agctgcaaga gatccatgat tcgtattttt cgtttcgaaa ttccaaagaa ggatggctta
     3241 ataatgtgat aagaagtcac tatattggtg ctggtatggc tttccggtct tctttaaaac
     3301 ccgatatttt gccgatcccg tcagaagtgc cgatgcatga tatgtggatt ggtttgctgg
     3361 gaggaaagaa gactgggttc ctaagagaac cgttaactct gtaccgaaga catgatttaa
     3421 acgcgagtga gattgaaaca cattctaaat tacggcaaaa aatttcttgg cgtgttcatc
     3481 tgattacggc gttgatcaaa cgaaaatggt ttaaacgttg ataaaagatg tttaaatcct
     3541 taatatttcc atgacaaata taacaattga tgagataata acaaagtgaa atttgaaatc
     3601 aaaaaggaga gcagatatcc atgaagggaa tcattcttgc tgggggcagc ggaacacgtc
     3661 tgtacccttt aacaaaggca acatcgaaac aattgatgcc gatttatgac aaaccaatga
     3721 tttattatcc aatgtctact ctgatgttgg cgggaatcaa agaaattctg attatttcaa
     3781 cgccacaaga tacaccacgt ttcaaggaat tatttggaaa cggaaatgat ttaggactgc
     3841 agatcgaata tgcagtacaa gaaagtccag atggattggc acaagcgttt atcattggag
     3901 aagaatttat tggagatgat agtgtctgct tggtattagg ggacaatatt tactatggtg
     3961 gcggtctgtc taaaatgttg caacgagcag cttcaaaaga tacaggcgcg acagtatttg
     4021 gctatcatgt caatgatcca gaacgctttg gtgtagtaga atttgatgag aatatgcaag
     4081 ctttgtcgat tgaagaaaag cctgctcagc caaaatcaaa ttacgctgta actggccttt
     4141 atttttatga caatgaggta atttctatcg ctaaaggtat caaaccttca gaacgtggag
     4201 aactagagat tacggatgtc aataaagcat atttggataa aggaaaacta tccgtagaac
     4261 ttatgggacg cggatttgct tggcttgata caggaacaca tgaatcattg cttgaagcat
     4321 ctacctttat cgaaacaatt gaaaaacgtc agaacttaaa agtggcttgt ttggaagaaa
     4381 ttgcttaccg tatgggctac atcgataagg aacaattgct ggcccttgca cagccattga
     4441 aaaaaaatca atacggtcaa tatttattga atttggccgc tgaataagga gtccttacag
     4501 atgaaagtaa cagatacaaa attaacagat gtcaaaatcg tagaaatgga cgtctttggc
     4561 gatcatcgcg gtttcttcac agaaagttat tcaaaagcaa aatttgcaga acatggattg
     4621 aaatatgact ttgttcaaga caaccattca ctatcagctg aagctggtgt ccttcggggg
     4681 cttcatttcc aaaaaggaga agctgcacag acaaaattga ttcgggtagt cactggagca
     4741 gttttagatg tagtagtgga tatccgcaaa ggtagcccga cttatggtca atgggacgga
     4801 tttatcttat ctgaacataa tcatcgacag ttgcttgtac ctaaaggata tgcccatgga
     4861 ttcgttactt tgacaccaaa tgtgaacttc ctttataagt gtgataatta ctataatgca
     4921 gaagcagatg ggggcatcgc atttgatgat ccagatttag cgattgattg gccaattgat
     4981 ccatcaagag cgattatgtc ggaaaaagat caccgtcatc cgacattaaa agaatttgaa
     5041 gcagaaaatc catttgttta cggagaaatc taagaattaa ggagaaattt tcttatgaaa
     5101 aacatcattg ttaccggagg tgccggattc atcggctcta atttcgttca ttacgtggta
     5161 aacaatcatc cagaagtgca tgtcactgtt ttggataaac tgacttatgc aggaaacaaa
     5221 gagaacttgg ctggacttcc tagcgatcgc gtagaattgg tcgtaggaga tgttgcagat
     5281 gcagaattag tggaccgtct agtaaaagaa gcggacgcag ttgttcatta cgcggcagaa
     5341 tcccataacg ataattcatt aaaagatcct ttcccatttg tacaaacgaa tatcattggt
     5401 acgtatacac ttttagaagc ttgccgcaag tatgatgtac gctatcatca cgtatctact
     5461 gacgaggttt acggcgatct tccattacgt gaagacctgc caggacatgg ggaaggcgaa
     5521 ggagaaaaat tcactgcgga aacaccatat aatccttcaa gtccatattc ttctacaaaa
     5581 gctggttcag atctgttagt aaaagcatgg gtgcgttctt ttggcttacg tgcaaccatt
     5641 tcaaactgtt caaacaacta tggtccttat cagcatattg aaaaatttat tcctcgccaa
     5701 gtaacgaata tcttaagcgg tattcgtcca aaattatatg gcgaaggaaa gaatgttcgt
     5761 gactggatcc atacgaatga tcattcttca gcagtgtggc tgattttgac gaaaggcaga
     5821 attggcgaaa cctatctgat cggtgcagac ggtgaagaag ataataagac cgtgatggaa
     5881 ttgatcttag aaatgatggg tcaaccaaaa gatgcgtacg accatgtaaa cgaccgtgca
     5941 ggacacgact tgcgttatgc aatcgattcg acaaaattac gggaagagtt aggatggaag
     6001 cctgagttta cgaacttccg tgatgggttg gctgacacga tcaaatggta cgaagaacat
     6061 gaagactggt ggaaaaaaga aaaagaggcc gttgaagcag catatgccaa aaatggacaa
     6121 taatacagag aaaatgtcac tgtattaatg gaaaaacaca tgtgaggcag gcgctcgcct
     6181 gtctcttttt taaaataaaa atcaaggagc ggataatcat atgtttttat taactggagg
     6241 aaatgggcaa ctaggtacag aattgcgcca tttattagat gaaaaaaatc tgcaatatgt
     6301 ttcaacagat gcaaaagact tggatatcac agatgccgag aaaacaatgg cttacatcac
     6361 agaattaaaa cctgatgtta ttttccattg tgcagcttac acagcagtag ataaagcaga
     6421 agaagaagca aaagaattag atgagaagat caatgttgat ggtacaagaa atgtagcact
     6481 tgcagcaaaa gctgtgaatg ctcaattcgt ctatatcagt acagactatg ttttcgatgg
     6541 aaaaaacaaa aatgtagaat acgaagtaga cgaccaaacg aatccactaa atgaatatgg
     6601 tcgtacgaaa ttattaggcg aaaaagcagt agaagagatt ttagaagact attatatcat
     6661 acgaacttct tgggttttcg ggatctatgg acacaatttc gtcttcacga tgcaacgcct
     6721 agcggaaacc cgcgacaaac tgactgtagt aaatgaccag tttgggcgac caacttggac
     6781 acgtactttg gcagaattta tggtatatgt catcgaagaa aaagcaccat ttggtattta
     6841 tcatttgtcg aacgaaaata gctgttcttg gtatgaattc gcaaaagaaa tattgaaaga
     6901 tacagatgta gaagttgcac ctgttacgtc agaagaattt ccgcaaaaag caacacgtcc
     6961 gcagtattca gtcatgagcc tgaaaaagac agaagagtta ggatttgtca taccaacttg
     7021 gcaagaagca cttgcgcaaa tgctggaagc tgccaaaaaa atgaataagt aatttccttt
     7081 tatcaaatag aatttggagc atgatatgga aaaacaaaca agaagtgcac gacgaaaaga
     7141 aaagccaaaa gcgaaccagg cagattcgaa tttggtgctt gacctgtttc taaaactccg
     7201 ctatcttatt ggcgttttag tcatcgtggt catcgttact tttaatttaa acggaagttc
     7261 acttggtgtt tgggataaat atgtgtcgca aagagacgat gggaaaaaaa ctgatgtgat
     7321 ttttggacag aatcgggaag tgcggtcaga cgaatggctg gtgcagacac ctttttacct
     7381 ctcacaagct gaagaggact ttccgttggt gaacgaggat tattcattaa acggacaaaa
     7441 catgatcatt gcgtataatt ctcctgtaaa ggatattacg gttatcggaa aaccattcaa
     7501 ctggggattc tttttcctcg gaagagatcg aggactttcc ttttattggg cgatgaaatt
     7561 agtcgtcatg gtcttgcttg gattcgaagt actgatgata ttaacgaaga gaaaaaaagc
     7621 tctatcgcta atcggtgcat ttgcgctgac attttctcca gcagtccagt ggtggttcat
     7681 gcaacatgtg ggcgatttga ttttctttac tttgggactg atgactgctt tttatcatta
     7741 tttctacaat catgataaaa aatggctacg aacgttgatg atgcttttgg tggttatttt
     7801 cggtattggg tttattttag tgatttaccc agcccatcaa gtgatgctgg cgtacttgct
     7861 gattttttat tttgttggcc tgcttattta ctatagcaag aaagtttcat gggatctgtt
     7921 ggatgtagtg ttgattggag gggcagtttt atttattggc ggagtcatgg ttcatttctg
     7981 gctaacgtca aaagacgcat tgttagcttc tttgaatacc ttatatccag gaaaacgggt
     8041 aagtactgga ggaaagtgga cgattggcga atttttttct ttccttacaa attggaaaat
     8101 tccttttgaa gatatcaact attcgaataa ctctgaagtg gccctgttct atcacttttt
     8161 tccaactgta ttcctagcaa gtccatttgt gttatttggc aaaaaagata gcgaacaaaa
     8221 gttactggga cgtattttga tgattttttg tctgttttct atcttttgga ttaccgttgg
     8281 gttgccaaaa tggctagcag aaatcactct gctgtcttat gttcctccat taagagccat
     8341 ccttactttt agttttgcgg cttgtttatt atcgatctgg tttatcgctt atatttggag
     8401 agaagatgtt attcctattt ggtataaatt cattttgttg gcagtaaatg catggtctta
     8461 tttctatgcg attacgcatt cgaaaatgga taattatttt tcggattttg aaaccatcgc
     8521 agttgctgtg ttgggaacag tgattttagc ttttgcatta tttggctgga gacgcttgtt
     8581 ttacctcgct tttgccgcct tagttatcgt atctggcttt ttcgtcaatc cagtcgtaga
     8641 aggtacagga gcgatttttg aaaaaacact ggcaaaagaa atccaaaaaa tcgatcaaca
     8701 agatcctggt aaagtttggt tgtctgaaga tgaattatat aacttcaccc caacattggg
     8761 agtgaaatcc ttcaatacag tacgctttta tccagatata gctgcatgga agaaaatcga
     8821 tccgaaagaa gaatacgaga agtattataa tcggtatgct catacaagag cttttatcgc
     8881 taaggaagat acaaagttca ttttagatcg tccagatatg ttttcggtca cgttgaattt
     8941 tgaagacgca gaaaaattag ggatcgacta tgtgatcagt aaacgaccat taactgatta
     9001 caaccaatta tatgaagcgc agttcacgca gctatatgga ccggataaag acggttatat
     9061 gatttttaaa gtagaatatc cggattatcc agatgtacca caaacacaaa ctcaggaaaa
     9121 tcagctagtc gtacagtaat aggctagctg agaataatcc tgaaatgtta gtagaaagga
     9181 gagtggagat aattcaagca atcgtacaaa aatttgtcaa caaaatgagt gtcggtttct
     9241 tgcttttgtt actgacgcta gtcttttttg tttggacagc agggttaggt tttccagcaa
     9301 ctttgtttat atataacaat agttcattac ttatcgcttt tagtgtggct attttgctca
     9361 ttttaaatgt ttataaagca aaaggcgtcg attttcttta tctaggtgtt gcgcttgccc
     9421 ttttcatttt cttttcttac tttcgggaat taagaaatga gtctttattt ggagacttac
     9481 tgtttccttt gattatttta ttttgtttag taattaaatg gatcgagtgg acaaaatggg
     9541 atcgcggtat ttatctgatt gtcttttcgg tcttcctcgg aatcacgtta tatcgtgtat
     9601 ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg
     9661 atatttggat caatacgaat ataatcggaa gttctttgat gactcttgct ttattgatca
     9721 gtggatttgc cagttctttt gaacgatggt atgtacgaat catcggtgca gtagcagttg
     9781 ttgcagcttt tctcgggatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg
     9841 tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta
     9901 ttgcttacac ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg
     9961 aaaaagtcaa tttgtttacc ggtcgagaag atatttggca taaattctat cagacgctag
    10021 gagaaaaatc tgaacaaatt ttactaggaa tgaaaacatt tatcttccaa cgaggaaacc
    10081 aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat
    10141 ttgggattgc tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt
    10201 tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta
    10261 tggaggatac attgacttca tacccatggg ttccgattat ttatatcctg ttggctatgg
    10321 ccgttcatcg atatgatgaa ccagaagttg cggaggaatt ctatgttatg gaagaggcat
    10381 atgacgatat gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt
    10441 ccaggatcca acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg
    10501 cagccttttt tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga
    10561 aaatcttgta tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat
    10621 ggtaaaatgg cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa
    10681 agatttgtat ttatttatga aggacattta tcagaacaga aaattattgc tacagttttc
    10741 gatcaatgac ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat
    10801 caatccgcta tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca
    10861 aatgatcgat gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt
    10921 cttcttctca gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt
    10981 aaagaaagtc gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta
    11041 cacccactta ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc
    11101 ttcagcaatg tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg
    11161 attgacttgg ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac
    11221 gatcgtgctg cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga
    11281 ttcaggtctt gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg
    11341 tgagtcattt ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg
    11401 ggttttcacg ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca
    11461 tttttcagat gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat
    11521 atcacaaaat cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat
    11581 ccttttaaga aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata
    11641 aaaaaaggtg agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg
    11701 aaaatcatta cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc
    11761 tcagctttgc tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc
    11821 tatttaaatg gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat
    11881 atcattgaat ttgctgacat cggagaccat atttatcaac ctgtaaaaac ttattcaagc
    11941 gggatgttcg ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga tattttgatt
    12001 gtggatgaag cattggcagt aggcgatctt gaatttcaac taaaatgtat ggaaaaattt
    12061 acagaaatca agaactcggg aaagacgatt ttattcgttt ctcatgatat cagttcgatt
    12121 cgtcgttttt gtgatcgttc ctattggttg aaaaacggag aagtcgtcga atatggcgat
    12181 acgatggaag taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg
    12241 gatcggaata aaaaccaagt agaacggttc gccgctccag atatcgtcga cgtgacgaaa
    12301 gcggaattgt tggataaaga caaaaatccg gtggatatgc tggaacaagg agaaaaacta
    12361 tacgttaaag taacttatga agtaaaagat gatacaatca aaaatccggt tttaggaatt
    12421 gcgctacgaa cagtcgacaa tttttatgta tgcggactta atactctact agatggtatc
    12481 aagattcctt ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa gttaggtttg
    12541 ttagctggtg aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg
    12601 gtatataaga caaagtacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc
    12661 gtggtcttag accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat
    12721 ggacctagat gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa
    12781 agttttagag tttggtcccg gaaacggccg attaacgaaa catttaatcg gagcaaaaca
    12841 atgtgaagtg agcattgttg agttagataa ggaattgttc gattttgtca gcgaatttgc
    12901 tcaagatgga ttttatggtg atatcgaaag ttttgagtgg gcaaactatt atgctggaca
    12961 gacatttgat tacgtcttgt ttgcggatgt tttagaacat ttggtagatc ctggaaaaac
    13021 attgaagaaa gtcagagaat ttttgaatga agaaggagag atcctgatta cattccctaa
    13081 cttggcgcat aattctgtca tgatcgattt gttcaataac caattgccgt gggcttctta
    13141 tggtctccta gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt
    13201 tgaaaaagcg ggattattca ttaatattga agattatttg tacttagctg taggtgatac
    13261 agaattaaaa tcaacttatg aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg
    13321 accatttggt gaagtctacc aatacttttt ctcattgatg aaacatccag tagcacaaag
    13381 ttccattgca gagccacaaa attctaatta tgtaaaagtg ttagaagtaa cccagcaaac
    13441 gaaacaagat gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac
    13501 actaagtttc cccgtatcag aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc
    13561 tagctttatc gaattcagcg cagaagctgc cggagaaaaa ctaacattta tcgattcgaa
    13621 tgctgtagtc aaaacagtaa atgattgtta tttgtttgat gggaaagaat taccagagtt
    13681 cgttctaacg gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg
    13741 ggaattgaca ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac
    13801 aaagcagctt tctgagaaaa atgacgaatt agaaagagaa aatcatcatc ttcttgagga
    13861 aaatacaaga ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga
    13921 aagtgatgaa tggacaataa aacatagact aggacgacgt aagaaagaaa cttcgaaaaa
    13981 aatccaagaa aaagaacttt ctatatgtat tgacgagaaa atctgggatg cagaaacaaa
    14041 aatattaaaa atcatcggat ggggaatcgc taattcggat cgccagccct tgtcctataa
    14101 gttatcagct gaccaatcgc catttttcga agcaattcct gtttcacgag aagaagtcaa
    14161 tcaagcagaa cagctcgcaa aaggaacaga agcgggattt gagttacgaa tcctttgcga
    14221 gcaagaacgt tcattcttag tggaagcagt agctcaaaat ggccaatcat ggatcattga
    14281 aatgtaaata atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa
    14341 attgccgtat ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg
    14401 ggctgggcaa ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat
    14461 gtgattgctg aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat
    14521 gttaagaccc aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagccatc
    14581 ttagacttcc aaacagcgag acatcaaaat ggcatagctg tgaaattaaa cggtaaatac
    14641 ccttatgatg acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg
    14701 ataaattatg caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg
    14761 aagccaggat cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa
    14821 gagcaaagga aagaagcaca aggatttgcg taccgtccat tgatttccat acttatgcct
    14881 gtctataacg ttgaaatcaa atggctcgaa aaatgcattg attcggtact ggatcaaacg
    14941 tatgatcatt gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag
    15001 tgtctggaat cctatcaagc aaaagatgat agaatcaaag tcgttttccg aaaagaaaat
    15061 ggacacatca gtctggcaac taattctgca ttggaaatag cagaaggaga atttatagca
    15121 ctcttagata atgatgatga attaccgcca tttgcattat atgaagtggc aaaagtgcta
    15181 aacgtacatc cagaactgga tgtgatctac agtgacgaag ataaaatcga tgcagatggc
    15241 aatcgctttg atccgcattt caaagcagac tggtcgcctg atacattgat gggaaataat
    15301 tatatctctc acttaggcgt atatcgaaca agcattgtta aagaacttgg cggtttccgt
    15361 aaaggctatg aaggttctca agattatgat ttggtccttc gtgtgacaga acaaatccct
    15421 gcagatcata tttaccacat tgaccgagtc ttgtatcatt ggcggacgat cccaggatca
    15481 actgcaagta atggcgaagc caaaagttat atctatgatt ccggagtgaa agcattgaca
    15541 gatgccctgt ctagaagaaa catcaaagga agcgtacgtc ctggcagaat ttctggattt
    15601 tatgagattg cttatgacgt cttgcaagag gatttggtca gtgtgatcat tccaacgaag
    15661 aatggctatg aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttattca
    15721 aattatgaaa tcattattgc ggataacgga agtacagatc caaaaatgca agaattattt
    15781 gcagaataca agcatcagct gaaagaccgc tttatcgtag aattgattga cattccattc
    15841 aattattctc gaattaacaa tttagcagca gaaaaagcaa acggcaaata ctttttattc
    15901 ttgaataacg atacagaagt gattgagcca gattggatga cggtgatggt ttcttatgca
    15961 cagtttgatc gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag
    16021 catgcaggag tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat
    16081 cgaacacatt gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact
    16141 gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca
    16201 ttagaagtag cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac
    16261 aatgtctatg cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa
    16321 gatactccag aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg
    16381 ccagcatata ttgcgcatga tccattttac aatgataatt taacaaaaga aggaatcggc
    16441 gacttttcgt tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg
    16501 cacataacgg ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa
    16561 ataagtcgag gttgggacag aagtgtttaa ctcc
//
LOCUS       DBNICI010000054        16090 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.054, whole genome shotgun sequence.
ACCESSION   DBNICI010000054 DBNICI010000000
VERSION     DBNICI010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.054"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..187
                     /locus_tag="AC1ZCC_001402"
     CDS             <1..187
                     /locus_tag="AC1ZCC_001402"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629664.1"
                     /translation="HVYKIFLTTRNEAALSQPLDKRRSISCPSVLAQKRQNMRSYFSI
                     FFSHTQVITLISQPLLS"
     gene            complement(182..601)
                     /locus_tag="AC1ZCC_001403"
     CDS             complement(182..601)
                     /locus_tag="AC1ZCC_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HIQ1629665.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(603..1187)
                     /locus_tag="AC1ZCC_001404"
     CDS             complement(603..1187)
                     /locus_tag="AC1ZCC_001404"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HIQ1629666.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1234..2709)
                     /locus_tag="AC1ZCC_001405"
     CDS             complement(1234..2709)
                     /locus_tag="AC1ZCC_001405"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HIQ1629667.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            2890..3201
                     /locus_tag="AC1ZCC_001406"
     CDS             2890..3201
                     /locus_tag="AC1ZCC_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629668.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3316..5652)
                     /locus_tag="AC1ZCC_001407"
     CDS             complement(3316..5652)
                     /locus_tag="AC1ZCC_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIQ1629669.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            5794..6462
                     /locus_tag="AC1ZCC_001408"
     CDS             5794..6462
                     /locus_tag="AC1ZCC_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIQ1629670.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERVGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            6465..7508
                     /locus_tag="AC1ZCC_001409"
     CDS             6465..7508
                     /locus_tag="AC1ZCC_001409"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HIQ1629671.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(7745..8626)
                     /locus_tag="AC1ZCC_001410"
     CDS             complement(7745..8626)
                     /locus_tag="AC1ZCC_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIQ1629672.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            8785..9513
                     /locus_tag="AC1ZCC_001411"
     CDS             8785..9513
                     /locus_tag="AC1ZCC_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HIQ1629673.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            9669..9986
                     /locus_tag="AC1ZCC_001412"
     CDS             9669..9986
                     /locus_tag="AC1ZCC_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIQ1629674.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            9979..10587
                     /locus_tag="AC1ZCC_001413"
     CDS             9979..10587
                     /locus_tag="AC1ZCC_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HIQ1629675.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            10642..11433
                     /locus_tag="AC1ZCC_001414"
     CDS             10642..11433
                     /locus_tag="AC1ZCC_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIQ1629676.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            11571..12059
                     /locus_tag="AC1ZCC_001415"
     CDS             11571..12059
                     /locus_tag="AC1ZCC_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HIQ1629677.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12110..12982
                     /locus_tag="AC1ZCC_001416"
     CDS             12110..12982
                     /locus_tag="AC1ZCC_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIQ1629678.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13158..13520
                     /locus_tag="AC1ZCC_001417"
     CDS             13158..13520
                     /locus_tag="AC1ZCC_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HIQ1629679.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            13529..14281
                     /locus_tag="AC1ZCC_001418"
     CDS             13529..14281
                     /locus_tag="AC1ZCC_001418"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1629680.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14295..>16090
                     /locus_tag="AC1ZCC_001419"
     CDS             14295..>16090
                     /locus_tag="AC1ZCC_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIQ1629681.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKK"
BASE COUNT         5001 a         2802 c         3296 g         4991 t
ORIGIN      
        1 ccatgtttac aaaatttttc ttactactcg aaacgaagcg gctttatcac aacctcttga
       61 taaacggcgt tcaatcagct gtccctcggt attagctcaa aaacggcaaa atatgagaag
      121 ctatttttcc atttttttct ctcatactca ggtcataacg ctgatttcac aacctcttct
      181 ttcataatgg tttttcacca tctttataat aaactggttc tttgatatgg aaatctattt
      241 tttccaacca atctaaaatc ttgtaaccta actcttctga catcggaaaa gaggcgttga
      301 tttgtgcatt ggagatatgg tcaacatgga taccggaagt gatcgtacaa cttcccggaa
      361 cggcagtttt gatgatcaca gctatttttt cggctaagac atcgtcttta tggaaccgac
      421 catcgtggct agggaaacgt acagtttgca tctctgtatc acgagaaaat gtagtgactg
      481 ttccgatatg tggtgtatct ccgcctgtta aagtgatcaa taagtcttta ccgattactt
      541 taacagcagc ttggatcgtg aaacctagtt tggttacttc aactatcgtt tctttttcca
      601 tattaatcac cctgccaacg ttttgaatgc tcatttacaa tttcaaattg atccaaaagt
      661 ttcattgtat ggtgattaac aagatcttgg atcgtttgag gctgattata aaaagcagga
      721 attggtggta tgatttgcac acctagtcgt gctaatttag tcaagttttc caagtgaatg
      781 gcatttaatg gcgtttcacg aggaacaatg actaactttc gttgctcctt cattgttaca
      841 tcagcagcac gtgcaatcag gttgtcgcct aaaccaacag agatacctgc gactgttttc
      901 atacttgccg gtacgatgat cattccatcg gtcaaaaatg atccgctggc gacagcagca
      961 cctaaatctt tgtttgaata atggtaatca aacaggcttt cgaattcagt tagtgataag
     1021 tcggattcca gctttaagtt ctgttttgcc catggactga tgattccgtg tgtttctatc
     1081 atcggatatt cttttagttt cttcactagg ttgatggcat aaatggtgcc tgaagcgcca
     1141 cttacaccaa tcacgatttt tttcttccgt tgtgccaatt gttccatttt ccgcactcct
     1201 ttttatgatg tcaaaacagt tgcagatcat tcattatttc aaatattttt cccaatcttc
     1261 tacttcttgg aattttgcac gtacaaaatg ttctttcata tcgaaaggaa ctgtaccgtc
     1321 aaagatagtt tttgcactta tcccacggaa acgaatagag ccaggattat attctggtaa
     1381 ttctgatggg tccaatggat ggttcctcat tcctgggatc acgatgatat ctttatctcc
     1441 ttggaaacga gtgttgaaag tccaaactac atcgttcatg tcgaaaatat caacatcttc
     1501 atcgactaaa atgactgtct tcaattcttt gaaggctgat aaagcaagaa tagctgcttg
     1561 acgctgaata ccttcatccg cttcgttttc tttacggatt tgcatgatgg tcatcaattt
     1621 accaccacct gcaggaggat tgtagacatt tactactttt cctggaatag ctttatcgac
     1681 caaggcaaga atacttgctt ctgtaggaat accggccata gatacgtgtt cttcactggg
     1741 accaatcgtt gtttgcatga ttggattgtc ttttcggtga gtaactgctt tgactttcac
     1801 tacttgtaca gctggattag catccccagt atagccagga aattctggca ttgcttttcc
     1861 tgagttcgtg ttgatatctt cttgcattgt ttcatttggc atgatttctg cttcaatgat
     1921 gaattcagca cgagcaatac ccacttcatc gactgctata ccatcaacga tttgaatagg
     1981 ttcgttacgc aaagctcctg cacaccatag ttcattgtag ccaagtggag tggtaggtgg
     2041 ttcgaaagtc gtgccaatgg caattgctgg atctaaacca atattgatag tgataggcat
     2101 tggtttattc aaggcttcga attgtttttg gaagtgacca atgtggcgtc cgccaggcat
     2161 gatgtacata ccgatcgtat ctttatcttc tagaaccata cgatggatcg ttacatcgct
     2221 cattgttttt tctggatctg atcctaagac tacacccatt gtgatgtatg gaccagcatc
     2281 ttcaggagta tttgtaggtg caggcaaaat gttgcgaata tcgaagtctg catcggtagc
     2341 tagatgaacg acttcttgtg ctggagcgtc tgctttgtct actttcacag gtttgattgg
     2401 attttctaca gcgtctttca agaaacgacc aagtgttcga taatcatggt gcaaaatttc
     2461 acctacacgt ttacgactag ccattgttcc gatgttcaca cgtgtgccgg ggaaaccttt
     2521 gatattgttg aaagtcatag ctggtccttc ttgagtcgga cgttttactg taccgccagc
     2581 accgatataa cggtaaacac cagataattc tgcttcagga tcgatttcaa tatctgtttc
     2641 gtgatattga cctggaagtg tttttagttc ttcgatgact tttcttaaat catacggttg
     2701 atctgtcatg agagattcct ccttagttgt ctttacctat ctatagtaaa aggtgaaaaa
     2761 gaggaaaaca attcaacttg ttcggtaaag tattctacaa tggaatggat gcgttttttt
     2821 gataatagaa taaactatga tatacttatc actaaaagaa agcgcatact tagtgttgga
     2881 ggaaggaaag tgaaaaaaat ggaggagtca tttcctaaag cagtaaaagt ggaaaatata
     2941 gcgaatattt tgaaagttac atttgaaaac ggagaagtaa aatacgtgaa atctcattgg
     3001 acagaagaga taaccgatgc cttgcaattt ggaaaaaaag gaagaggaaa aagaaaaaat
     3061 cttctcgcac tttctcgtaa tatgtggatt gggactgaag taacgattga agcagatgga
     3121 acagtattca taaatggaaa agacaggtat acgccagaag agttgtggta caaaggaaag
     3181 aaaagtatac cagaattata aaaaataatt aaaaaaaaca gcgcgaaaca aggaacatat
     3241 tacttcctcg tttcttgctg ttttctacta tttcagcgct cttatgactc aaatctaaat
     3301 gtggttgtca caaccttatt ctgcacgcaa agcgatagcg gcgtcttttt tcgctgccat
     3361 tcgtgcagga agatgaccgc caagcatcgt caatatagta gagacgatca ctagaataat
     3421 cgcatggaca ggatttagtt gagcaacatt ttttagatcc gtcatgttgt ataagatgct
     3481 attgataggg aacgtagcta gccatgcaat aaacacacct aatatcccag aagaaatacc
     3541 taaaatacac gtttctgcgt caaatacgcg agtgatgtct tttttacgtg cacctaaagc
     3601 ttttagtaca ccgatttctt ttgtccgttc aatcacagat gtataagtaa tgatactgat
     3661 catgatcata cttgttacta aagagatccc tgcaaaagca ataaggacat aagtgatagc
     3721 atccatcaat ccgccagtca gttcagtcat cgttccagct aaatcagtat aaatgatttt
     3781 atcttcatct gatttgcctt tattgtaatc atctagataa tccaagatct tttctttgtc
     3841 ttcaaaattg ttcggataaa tcatgatact tgaaggcaag ctgtctccac ctaaataact
     3901 aagcaatgtt tgtttggcat tttcgtcaac tttttcagta gttaagacat ttacaccact
     3961 gtctctttga gctttgacga tttctgattc tttgttttcg ttgacgattt gagtcgttaa
     4021 ttgatcgcta taagcgattc ctggagataa gagattcatt gtagaagaag atttcacgcg
     4081 taggatcccc gagatcttca attcatcaga agcattctga tacatcgcat cataatctgt
     4141 attagggata aaattcccag ttggaagttt tgtatagtaa gtattgttgt ttactaattt
     4201 gaatgttgta ccaacgatat catcaaaatc aagtttttcg tcttctttca catcaaatcc
     4261 aagattcttc aatgcgttga tattagtatt attattgccg tctacgatca gcacgacatc
     4321 tgttgctgag gctggataac ttccggcaag taaagaataa ttgtctttta aaaagttttc
     4381 tttgctagtg tcaagctgtg tagggaaact tgacactccc acacctgtca ttgctgacat
     4441 cgtactagat aaagaaagag attccgtatc aggattttga ttcgagaagc tgacaggctg
     4501 tactttgcca tttacatcac gtaaaagatt gattcctgta gtacgggtaa atccgatatt
     4561 attacttaag ttaggatcga tgtctgttac gtaatcgacg tatgtttgat caatcttatt
     4621 cgtgtgctgt gctttatctt cttcgctgac ttttgcagta atcgttttgc tgtctggata
     4681 atccgctttg cttgcaccca atccggcatc gtcacgagtc tggttagtcg ttaccttaga
     4741 aatagtaatc ggaaatttcg ccatcgtttc agcttgcgtg ttatcgattt gtttttggaa
     4801 tccagaagaa agggacagaa cgatcgcgat tccgatgatc ccaatactgg aagcaaagga
     4861 ggtcaagaaa gtacgtcctt tttttgttcg gatattgttg aaggaaagct ttaacgctgt
     4921 ccaaaaactc atctttgttc tgcgtaagtt aaattgatca tcttttggtc gttcgatgtg
     4981 aggatgggaa tctgttaaga tcttaccatc agaaaattca atgattcgat ctgcgtattg
     5041 atgggccagt tcaggattat gtgtgaccat gatcactaat ttttctttcg ataactcttg
     5101 gatcaattcc atgatctgga tacttgtttc tgtatctaac gctccggttg gttcatcgca
     5161 caaaaggata tctgggtcat tggctagtgc tcgagcgatg gccactcgct gcatttgtcc
     5221 accagataac tgatttggct ttttatgcat atgatttgtc aagccgactc ggtgcaatgc
     5281 atcttctgcc cgttttcgtt tttcgtcttt agaaacacca cttaatgtca tcccaagttc
     5341 aacgttttca ataatgccta gatgaccaat caagttgtaa ctttgaaaaa tgaaaccgat
     5401 agagttgttt cggtaggcat cccagtcact gtccttaaag tctttagtag actttccgtt
     5461 gattaccata tcaccggaat cataattatc tagtccacca atcacattca gcatagtcgt
     5521 tttaccagaa ccactaggac ctaaaatcgc tacgaattct ttttgtctga aagcgacaga
     5581 cacaccatcc aatgctttgg tggtggtttc gccgacttta taatattttt ttatatcttt
     5641 taattgaagc ataatcaccc ttctcccgta ttgaaatatt cggtatacta ataagtatat
     5701 cagaacaatg atcgtagtat acgaaataaa tgttaactga agatgaacta caagaaaaaa
     5761 tctagaaatt tttcaaaaaa cagagggaga tcaatgaatc gtattttagt agtagaagat
     5821 gatccgcaat tacagttgct ttaccgttct gtgttggaac gagtgggctt ttcagtcatc
     5881 accgcattga atggagagga tgcgctgaaa aaactagaag cgacacgtat agaattaatc
     5941 attacagata tcatgatgcc gaaaatggat ggctatgact tgctagactc attacgtagc
     6001 accagatttg acacacctgt tctaatgatt acggcaaagg ccgattttga ggataagaaa
     6061 cgcgggttca aattaggagc agatgattat atgacaaaac ccatcgatgt caatgagatg
     6121 atcctacgtg tggaagcatt gcttcgacgt tcaaagatca attacagcag tagcttgata
     6181 atcggacaaa caaaattaga gctagatgct tatacagtgt tacaagatca gcattcgttg
     6241 attctgcctc aaaaagaatt tctgcttctg tataaattac tgtcttatcc caacaagatc
     6301 ttcactcgcc aacagttgat ggatgatatt tggggattag atacgaatac agaagaacga
     6361 acgatcgatg tacatatcaa acgtttaagg acccgttttg aaaagacgga agattttcaa
     6421 atcatcacag tacggggact aggctataag gcggttattt aaagatgaaa aaaaagctat
     6481 tttcacaatt atggatctat tttgctagta ttgtcttcat ttcgatctta gtgacactgc
     6541 tgtgttttct cggttttatt ttcttattgt ctcgacattc tatctcgcct gctgaatcgg
     6601 ctggaaaacc aacgttatac ccgttattcg tctttgcggg gttcagtatg gtcgtgggta
     6661 caggaatatc gatttttgtt gggcggcgta tcctgcatcc aatcagtgcg ctagggacaa
     6721 atatgagtct ggtagcaacg ggagattttt cgattcgaat ggatgaacag cagaaagtag
     6781 cagaagtaca gcaactgtat aaagatttca acgttatggt ccaagaactc aatagtatcg
     6841 agacactgag gaatgacttt gtgtccagtg tatcccatga attcaaaaca ccactagcaa
     6901 caatccaagg atatgttcaa ttgttacaag ccccaaatct ctctgacgaa gaacgccaaa
     6961 tatttttata tcggattatc gaaagtatca cgcagctttc acaattaaca gagaacatct
     7021 taaaattgaa caaactggaa aaccagcgta tccaactgga aaagaaagag tatcgattag
     7081 atgaacaaat cagagaagta atcgtttttt ttcagcctaa atgggagaaa gaacagctag
     7141 aattggatat cgaacttgct gcagtaaatt acacaggcaa tgaagagttt ttgtaccaag
     7201 tgtggctgaa tatcatggat aacgccataa agtacaatca aatcaatgga cagattcata
     7261 tcaaactatt tgagacagcc acagagattg tactggaagt gaccgattca ggtgtaggaa
     7321 tgaatgaaga gaccagagat cgaatgtttg aaaaatttta tcaaggagat acaagtcgtc
     7381 aaatttctgg gaatgggcta ggactttcac tggttaaaaa aattctagaa ctgcatgatg
     7441 gacgtattga ttatagcagt atcgaaggag taggaacgac agcaatgata cggttaagta
     7501 aacaatagga aaaagaataa aacccctaaa aattctattg aaggttttta ggggttttta
     7561 gtcgtttttt tgtgattagg gaaatgttct tacggttctt ttggcataaa aatcgctgct
     7621 ttcttattga actacgagaa atgttagagg tgtcattgaa acaaggacat gatgcaagaa
     7681 agagagcgaa agtcatgcgt cgctctcttt ttttgggtgg atagatggac agaaaatgta
     7741 gggcttagtt ttcttcttga tttaaatact cccaaaatgt atctgaagcc aaagaatgct
     7801 caccgacttt acgagtaaat gcaagataac tctctgttgt tggagaaata gggagaatca
     7861 cagtattttc ttttaataga ttcttgactg ttttttccat aacaatagca atccccatgt
     7921 catttgcaat catattgatg atcaagtcga ttcttgctcc tttataagag atttttggtt
     7981 caaatccagc ttcgcgacac agatctagta caggttgata caagttcgta gtggggccaa
     8041 ggagcaaaaa gttctccttt ttcagttcgc ttagatccaa tttcttttta ctagctaaag
     8101 gatgattttt aggtagaact gctacaaaat cgtctgtttc agtaggcaac cactcaatcg
     8161 aacttggtgg ggcctggaaa cttctagcaa agtggatcgt gtcactatgt tcattggcgt
     8221 cattgtaagt taattcgaca ctttccactt ctttcaactg gactgtaaat tcaggatggc
     8281 gtttcaaaaa ctcggtgatt ttggaaaaac ctttataatt gatcattgtc ggtatcgtta
     8341 agatatttaa tgaaagattg ttgtctttcg tataggtttc aagcacttct tgcatagcat
     8401 ggtattgttc tacgacattt tttacatatg ggagcaataa gcttccagct tcggtcaatt
     8461 cgatttttct atgggcgcgt tcgaataatt tgacgtctaa ttctgtttct aacgaaagaa
     8521 tctgtttaga aatatttcct tgggtggtga attgtcgatc agcagttttg gtgaaattca
     8581 ttgtttcggc taaatcaaga aaaacttcga atttatggat atccatcata ttcctcctac
     8641 ttattccaat aaagaatact ttaacacaaa aaatgaattg gtgtaatctt ctctgtctgt
     8701 tacaatcttt cttgcagcga aagcaataat tacagaaatt tttctgattt ttttcgcttc
     8761 gtagaaaaag agaggagttt tttgatgcca actggagttt tgatcaatgt aggttccgtt
     8821 ttgtttggcg gactaattgg cggattagta ggaaataaat taagtgaaca ttttaaagca
     8881 cagctgacga tggtttttgg tgtgtgttcg atggggatgg gaatctattc cattgcgccg
     8941 atgaaaaata tgccggctgt tatttttgct ttagtgatag gaacagcgat tggtttgatt
     9001 gttcatttag gtaatgggat aaataagggg gctgcgctga tgcaagtacc tatttctaaa
     9061 atcttcccta gtgaaaagct tggtatgacc cacgatgaat ttatcagcac attagtgacg
     9121 gtcattgttc tattttgtgc aagtggtacg gggatctatg gaagtttaga ttctgggatg
     9181 acaggagaca gtacgatttt gatttctaaa tctgtccttg attttttcac agcagctatt
     9241 tttgcgtgta atttagggta tgtcgtttct gtgatagcaa ttccccaatt cattattttt
     9301 tatattttgt tcttattggc aaaatttatt tatccattga ctacaccaga catgatctta
     9361 gattttaaag cttgcggcgg attcttgatg gtcgctaccg gttttagaat gatcaacgtg
     9421 aaaatgttcc cagtagctga catgatacct gcaatgatcg ttatcatgcc actgagctgg
     9481 atgtggacaa attggattat gccactgtta taataaaaat caaagagaag ccattttttg
     9541 ctgactaaaa aggatcaaat cttttagtca gtttttttgt atttgacaaa ctgattttga
     9601 aactatatta tatgttgtat aatatagatt gaaataaaat attacacttg agaatagaag
     9661 gtgacgtaat gcacatccag attccaactg ttcttcttga tggtactgtg ctagcagcat
     9721 taagcaagga agatatgtat gggtatgcct taacaaaaat cgtacaagaa agtctctctg
     9781 tcagtgaatc tacactatat ccggttttga gaagattgaa aaaagaagga tatcttgaga
     9841 cctatgatga accttttgaa ggcagaaacc gtcgatatta ctcgttgaca ccaatgggaa
     9901 gtcgtcattt agaagaaatt attgatgaat ggattgtttt tcgagattct ttagaccggt
     9961 tattggagga gagcacaaat gaatgaatat ttgaatgaag ttgtggatca tcttcaagaa
    10021 attcctgaag acgatcgttt tgatttgatc cagtattatg aggaatattt tttagattct
    10081 ggaaaaacat tagatcagat cattgatgaa tatggtacac caaagcagtt tgccttaaaa
    10141 ttaaaaatca gttacttttc agaagaagaa caagcagaaa acgagttagt gaaaaatcca
    10201 aaaagacaaa ttcgcctgat ttggttaatc attatcggtt tatttgcttc tcctgtcctc
    10261 attccactag cactggggtt tatattggtt attgggggac tattggttgc tttactagcg
    10321 ctcattttta gtatctatgt tttatgtatc agtatcttag gtgttggcct attcatgatc
    10381 atctcaggaa tcagtgtgtt tggacaatcg attctgagtg gattattctt tatcggtgtc
    10441 ggtctgttcg ccacaggaag cgcagtattt atttcgccac tgttgctgaa aggaacaaaa
    10501 gggctttttt atgtcatgat gaactttgtt aaatggatgg gacgacgttt tattacaaaa
    10561 agagatctgc attcagcggg cgtttgaagg aggacacata aattgagatt gaaacatttt
    10621 ttgattaccg gtattggtct gatgcttact ggtggcctcc tagcaggtgc aacttataca
    10681 ttaggcgctc aaaagagtct gacttgggat aatggtcctc ggttgatcga tatggttact
    10741 gagacaaaaa aaatcaagga tgcaaacaaa atcgtcatcc atgcagaaaa tcaaacagtt
    10801 tcggtcatac gcggattgga ttttgagata aaaacgactt atgataagac agaaaagcca
    10861 gaaataaagg agaaaaatgg cgtgttgact gtgaatgcag tcaaaaaatc aacaggtgca
    10921 atcattagtt tagaagacac aagtgcacaa atcactattg ttgtccctgc agatcatgaa
    10981 ttatctgaat tgacaatcga agggaataat agtgcaattg atatcaacga agtatcaatc
    11041 aaaaaagtga atactaagac acatgatggc tgggtatcgt tcaatgaatt tacaggagga
    11101 acaataactg cgaaagatga attaggctca cttcgattag atgaggccaa tgtggataac
    11161 ttgatcgtca caggtgaaaa cagcaacttc catctagaag acagtttgat cagcaaagaa
    11221 ggaaaagtca tcctaaacgg tggcaattta tctttgcatg agatggaaaa cagtggctat
    11281 actatccaaa cgtctggtga atcttatatt gaaaaagact accagccaat aacaaatact
    11341 ttgttagaac aaggacagaa aaagctaaag atcactgcta aagacacatc gatcaatatt
    11401 acgacggatc aagaggacaa tgaaacagat tgatcctgta ctcaactgga tattttaaga
    11461 aaaaacagca aagatccttc caatctttgc tgtttttcat cgcttaacgc acataactat
    11521 agtaaaataa gaaagaaaat atctaaaaag aaaacagggt gaaagagtat atgaaggtag
    11581 aagaaggtca aagagaaaag ttaaaaacag aaatggaaag attacatact tatatcactc
    11641 aactaagcca aaccttctat gatccagaca aagaaaaagt gatggttaat tacccgaaca
    11701 attcagaagg tcgccaatta gagcaagtgt accatgaagt tttcaaacat ttattgacgg
    11761 tgaagaaaga gctggattat tattctttac cgattatcga tacaggaata ttgaagtatg
    11821 atgggaagaa ggaacgattt atctttaaat ctgttcggga agatttgcct ctttcagcag
    11881 ggatggatct agaggtcttg gtagaagact attttacaga agaaaagcac tgggttcgaa
    11941 caaaattgga ttatttaccg caggcagccg gagggacaca ggcaagcggt tggtacatca
    12001 ctgaagacaa agagttagaa ctagaaggtg taatggcgcg tattcgaaaa aaaaattaaa
    12061 aaagtaacat tagatattta ttgaaggata aaggagcgca ggaagtagca tgagagaaaa
    12121 aagagcattt tatatcaatg gttatgcagg gattgtcgga ttggtcgtat tagctttagt
    12181 tggattgttt ttcttctatt taggtatgtg gcaagcgaaa gtttgggcac tttttttaag
    12241 tatttttctt tggttgatca cgttattgtt gttaagttcg gcaactgtag tcagtcctaa
    12301 tcaagcaaaa gtaattttgt tttttggaca atatttaggt acgattcgag aaaatggctt
    12361 tttccttacg atacctttgg cacaaaaaat gactgtttcg ttgaaagttc ggaatttcaa
    12421 tagttctgtt ttaaaagtca atgatttgga tggaaacccc atcgaaatct cagcagtagt
    12481 agtctttaaa gtcgttgata cagcaaaggc attattcgat gtagcgtatt atcaagattt
    12541 tgtagaaatc caaagtgaga cagccattcg gcatatcgct agccaatacc cgtatgatac
    12601 attcaatgag aatgatttga cgcttcgagg aaacacgacg gcagtgtctg acgagttgca
    12661 aaaagagctg caagagcgtt tagcagtagc aggtgtcgaa gtaatcgaaa cacgcttgaa
    12721 ccatttagcc tatgcaacag aaatcgctag tgccatgctc caacgtcaac aagcaaaagc
    12781 cattttgtct gctcgacaaa ccattgttga aggagctgtc acaatcactc aaatggcctt
    12841 agagcaaatc gaagatggac aagaaatcaa tttcaccgat gatcggaaag tccaattgat
    12901 caataattta cttgtctcaa tcattacaga caaaggaacg caacctgtta tcaatacagg
    12961 cgacatgaaa gaccgtaagt gacatacttt ttgtaagata ggaagaagga gtttaggacg
    13021 gattattgtc ttgaactctt tttttagttg ttttaaagaa aatgacaaaa ttgaaagaag
    13081 aatgatagtt gattcgaagg cagaattgtt cgcttttttc tataatcagt atataaagta
    13141 agagagaagg ggaaaaagtg aaaaaacttc tgatcggtct acttgttttc atcgttgtgg
    13201 tacttgcagg ggtgaaaatc gcagattacg tagttatggg tggagaagaa tactatgtac
    13261 aaattacgac agatggtaca aaaacagttt caaaatcaga ctctggacaa gaaacagtca
    13321 actatagata tatcctgcct ggctatacga aagagggtaa ggaaaaagaa ctggacttca
    13381 agggacaatt gactcgaccg cttcgtaaag gagcatattt aaaaatcacc tggaacaaaa
    13441 ataaaggtgt cacttcgtat gaagaagtcg aaaaaaaaga cgtgccgaaa acagcactgg
    13501 aaaaactaat taaagggtag ggaaagaaat gaacaaaata gtcgaagtac agaatgtaac
    13561 aaaagtatac ggaaaaaaca atgagaaaaa gacgcaagca ttaagcggca tcagcttcga
    13621 tgtagaaaaa ggcgaattta ttgggatcat gggcgcttct ggttctggta agtcgacact
    13681 tttgaatatt ttatccacat tagataaacc aacagatggg catatccgaa tcaatcaaaa
    13741 agatgtgacc acattaaaag gaaatcaact agcagatttt cgtgcgaatg agattggctt
    13801 tattttccaa gatttcaatt tattagaaag cttgacagca caagaaaata tcgctgttcc
    13861 tctttcttta caaggcgtca gaccaaaaga aatcaagcaa cgtgtccaaa aaattgcaga
    13921 acgtttatcc atctctcata ttttggaaag ctatccttct gagatttccg gtggacaaaa
    13981 acaacgggtc gccgccgcac gtgcattgat cacacaacca acgattctat taggggatga
    14041 gcctacaggt gcgctggatt ccaaaagtgc ccgggatctt ttagatacga tggacgaatt
    14101 gaatacaaaa gatcatgtat cgattcttct tgtgacgcat gatccgtttt ctgcaagtta
    14161 ttgccaacgt attttattca tcaaggatgg cggtatccat caagaagtga aacgtggaga
    14221 tcaaagccgt gattccttct atcgagaaat actatctatt cttggaaact tggaacagta
    14281 gggggagatt gactatgctt tggaaattat cacttactgg gatcaagagc cgtttaagag
    14341 actacttggt gttattctca ggattagtta tggcatctgc tattttttac atgtttgaat
    14401 caatggcaag caacgaagct tttttgaaaa gtaatacgat cgttgcggct acagttatca
    14461 ttttccgatt cggttctgtt ttactaggga tcatcacatt cgtttatatt ttatatgcaa
    14521 attccttttt gatgacgatg cggcaaaaag actatgcaat gtttatgatg ctgggagcaa
    14581 aaggcagaaa aatcgcacag atgatttttt ctgaaacatt cttagtaggg atcgcagcta
    14641 ctttagtggg ttcagctgtc ggtgtaggat tgacaagtat cgtccaccga ttattagtgg
    14701 atcagttaga tctcaatatt tcgcatttta caccttttag tattcacggg ttgttgatca
    14761 ctgttttgtt ttttgctgtt ttgtttttat tagctgctgt tgtcaatgct ttttcgattg
    14821 tcaaaaaacc gattttagca ttgattcgtg cggatcagac accaacaaga atcaagcagc
    14881 ataaattctt gtttttcctt gaagtagtac tgggaattgc ttttctaggc tgcggttact
    14941 atatgatgaa acacgtgggg acctatcaag tctttggtat aggggtagcc ttagttacga
    15001 tcgtattagg aacttatttc attttccatt ctgtgatcat tttcttttta tcattactaa
    15061 agaaatcaga acagatttca ttgaaaaaat tgaacaattt cacattgtca cagttaaact
    15121 ttcgtattcg tgaatataca caaatgctct caatggtcgc tatgctgttt gccttggcac
    15181 tgggcgcttt gacggtagga ttaggtttta aaaatgaaat tgcagacttg acagaaaaca
    15241 attctggata tgatctagtg atgaataatc ctcgggcaga agatcagcaa aaaatcaaag
    15301 aactttcacc aacactttct gcttcttata ctcaaaaaga agatgcggga acgatttact
    15361 ataatgcaag tgaatttgcg gctcaaccat tgatcatgat aaaacatgaa gatggaacac
    15421 cgccgaagat cacgaaagaa aaagttcctg ctaaaaaaat gaatgaactg actgtacaag
    15481 aggaacttcg aagctatgaa ttgccggaac agcaagaaaa agaaatcaag gtagtcagtc
    15541 aatcagaatt tgatcaactg aacttgccac aatccacttt acagctagtt caggtaaaag
    15601 acttccaagc aaacctgaaa ccaatcgaaa cacttgttgc tcaaaataaa atgaacaatc
    15661 catctttgca aaaagtagag tacagcaacc agaaatatga aatgtatgaa gtgtataatg
    15721 gtctattctc tggttttgaa tttatgggat tcttcctagg tttagctttc ttgaccatgc
    15781 ttgctagctg tttgatgttc aagatcttgt caggaagcaa gagtgacatt gctcgttatg
    15841 aaatgcttga aaagatagga acacgacgcg ggttgcttaa acaatcgatc cgtagagaag
    15901 taggcgtgtt attcttagca ccaggaatct taggtgtagt ccatgtctta ttcggattac
    15961 aaatgtttgg tctattcatg caaaatccat acaaagatat ttgggttccg tttacgatat
    16021 tcttcgtatt gtatttcatc tactatgtac tgacgacatg gttatatact ggaatcgtat
    16081 tgaaaaagag
//
LOCUS       DBNICI010000055        15915 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.055, whole genome shotgun sequence.
ACCESSION   DBNICI010000055 DBNICI010000000
VERSION     DBNICI010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.055"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(328..2055)
                     /locus_tag="AC1ZCC_001420"
     CDS             complement(328..2055)
                     /locus_tag="AC1ZCC_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HIQ1629682.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2048..3241)
                     /locus_tag="AC1ZCC_001421"
     CDS             complement(2048..3241)
                     /locus_tag="AC1ZCC_001421"
                     /EC_number="7.6.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015697 - quaternary ammonium group
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary amine ABC transporter ATP-binding
                     protein"
                     /protein_id="HIQ1629683.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3428..4072)
                     /locus_tag="AC1ZCC_001422"
     CDS             complement(3428..4072)
                     /locus_tag="AC1ZCC_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIQ1629684.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4166..4300)
                     /locus_tag="AC1ZCC_001423"
     CDS             complement(4166..4300)
                     /locus_tag="AC1ZCC_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HIQ1629685.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4302..6434)
                     /gene="feoB"
                     /locus_tag="AC1ZCC_001424"
     CDS             complement(4302..6434)
                     /gene="feoB"
                     /locus_tag="AC1ZCC_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HIQ1629686.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6431..6904)
                     /locus_tag="AC1ZCC_001425"
     CDS             complement(6431..6904)
                     /locus_tag="AC1ZCC_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIQ1629687.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7305..7529
                     /gene="nrdH"
                     /locus_tag="AC1ZCC_001426"
     CDS             7305..7529
                     /gene="nrdH"
                     /locus_tag="AC1ZCC_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HIQ1629688.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7688..9847
                     /gene="nrdE"
                     /locus_tag="AC1ZCC_001427"
     CDS             7688..9847
                     /gene="nrdE"
                     /locus_tag="AC1ZCC_001427"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HIQ1629689.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9897..10862
                     /gene="nrdF"
                     /locus_tag="AC1ZCC_001428"
     CDS             9897..10862
                     /gene="nrdF"
                     /locus_tag="AC1ZCC_001428"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HIQ1629690.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10951..12090)
                     /locus_tag="AC1ZCC_001429"
     CDS             complement(10951..12090)
                     /locus_tag="AC1ZCC_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIQ1629691.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12399..13112)
                     /locus_tag="AC1ZCC_001430"
     CDS             complement(12399..13112)
                     /locus_tag="AC1ZCC_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HIQ1629692.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13366..14067)
                     /gene="nagB"
                     /locus_tag="AC1ZCC_001431"
     CDS             complement(13366..14067)
                     /gene="nagB"
                     /locus_tag="AC1ZCC_001431"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HIQ1629693.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14301..15833
                     /locus_tag="AC1ZCC_001432"
     CDS             14301..15833
                     /locus_tag="AC1ZCC_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIQ1629694.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
BASE COUNT         4929 a         3277 c         2794 g         4915 t
ORIGIN      
        1 aattccttct tgaaacgtag caaaataatt cctgaataag aatttaatca cttttaagta
       61 tataaactat gtatattctt taatattttt tgagaatgca cttcgtacat aataaacttt
      121 aatcacatta gtctttcata cctacgatcg atctctttga ctcgcaccca taaattcata
      181 atagtttgtc ggcgtctttg aacgtttcga agatgagcta cgcattctct ttttttatag
      241 acaaaaaaga tagagacgaa catggagctt ccaaatttgg atatttgatc caattttgga
      301 agctccattt atttcgtgtc tgtcttttta ttttttccag ctttcgacta attcttggtt
      361 ctctttgatc cagtcttttg ctgcttgctg tggatctttt ccattgttga tttccaacat
      421 aactttttcc atatcttttt ctgaccaatg gaaattgtct aatactttgt aagcttctgg
      481 ctggtcttct ttcaatcctt ttcgaaccat cgtatggatc tgctcttcgc ttcccattcc
      541 gttttcagga tcttctaagt atttcagatc atatttggca aacatccaat gaggtgtcca
      601 tcctgtaact acaattggtt cgtgtttttt gatcgcttga cctagtgcaa cggtcattgc
      661 tcctgaagaa gatgtctcta ctttccagtc tttcaagttg tcatattttt ggatcgtgtt
      721 ttcagcagcc gtaactacac cagctcctgg ctcgattcct atgattttct tacctgcctg
      781 atcggttaac tcatcgatcg aatttacatc catgtaggct ggcactgcta aaccaacttt
      841 tgctcctgtc aaattggcac ctaggtcttc gacttgatct ttatattgtg catattgtga
      901 accatgagtt ttaggtagcc aagctgaaac catggcatct gactcgccat ttgaaacaga
      961 tttccacatg attgaattat ctaatggcgt catcgttaca tcatagccca tttgtttcaa
     1021 tacttcaccg actacatttg ttgaagcaac ttctgtatcc cattcaacat aagataagtt
     1081 gatacgtttt gtttctttgg cagatgaaaa actccctata ccaatcgcac ctaagatcac
     1141 tactactaca gcaccaatga tcagtccttg acgtttttta cttttctgtg ttgttgctcc
     1201 tgcagctttt ttattgtttg gctggttcaa atgctgtgta aaacggtcaa tgatgattgc
     1261 taagataact aatgcaaccc cgttgacaaa tccattcccg acttgtgctc tttgcaatgc
     1321 tgataatacg ccgcgtccta agccgggtgc accgatcata gaggcaataa cgaccattga
     1381 aagtgacagc attgttgttt gattgatccc tgccatgatc gtacttttag ctaaaggcaa
     1441 ttctagtttg aatagttttt gacgacttgt gctcccgaat gaatcagaag cttctactaa
     1501 ttctttagga acttgtcgaa tccctaaatt cgtaaaacga acggttggtg gcagagcaaa
     1561 gatgactgag gcaaataccc ctggaaccat cccgataccg aaaaatgcaa ctgctggaat
     1621 caaataaacg aatcctggca ttgtctgcat aaagtcaagg atcggtttga taatactttg
     1681 ggctttactg ctttttgcca tcaagatccc taatggtacc ccaatgatga tcgagatcac
     1741 actagataaa agaacaagtg tgacagtatt catcaaatca ttccatagac cttggttata
     1801 aataaaccat agaccgatta atgtgaacaa cgtaagtcct ggacgtttct tagaaataaa
     1861 gaaagctgct accgtcaata acaagataaa caaaggagct ggaatcacta acagtaaatt
     1921 tgtaatcccc gacatcactg cctgtccaat cgtttgtaaa aatgaaaata ggccagaaaa
     1981 agttgtcgta aaccattctg tgatattttc tacccagttt gccactggta attgatagtt
     2041 caatagatta ttcatgttca ctcacctcgc ttgtttctgc taatgcttca atcacactac
     2101 ctcggatgac gacacctttt aattttccat cctctacaac tgcaagtgga gtcggtgagt
     2161 cataaataat attgaaaata tcacttacca ccatgtcttt cccaatcacc gtcacatttt
     2221 catctaggaa gtcgatcaat ggacgttttt gcttcctagc ttctaacgct tgatctgcag
     2281 tgatgatccc tttcaggtgt ctctttctgt caacagccat cagcatgctg acttcttcat
     2341 tacgcatcct tgtcaatgca acgtttggtc catcagattc gatattcgtc gtcaatgcag
     2401 gtaccatgat gttttgagcc gtaagcactt tggaacgatc gacttcttcc acaaattcac
     2461 gtacataatc atttgctgga ttggtcaata tttcttcacc tgttccgatc tgcatgattt
     2521 cgccgtcttt catcaaggcg attctgtccc caatccgtaa agcttcattc aaatcatggg
     2581 taatgaaaat aatcgttttt tgaacattgg cttgcaggtc cagtaattcg tcttgcattt
     2641 ctcgccggat cagcggatca agagccgaga acgcttcgtc catcagcaag atctcaggat
     2701 catttgctaa agctctggct aggccaaccc gttgttgcat cccaccagat aactgactag
     2761 gatattggtc tttaaatgaa agcaaactag aattttctaa tgctttttct gctttttctt
     2821 gtcgttcttc tttaggtaca ccacggactt ctaacccgta ttctgtattt tccaagatcg
     2881 ttcgatgtgg aaacagaccg aagttttgga aaaccatatt gattttatga cgacggactt
     2941 ctcgcaattc ttctttgctt aacttcgata catcttctcc atcaatatag atactgcccg
     3001 aagttggctc aatcagacga ttcaatagac ggatcaatgt ggatttccca ctcccggaaa
     3061 gccccatgat cacgaagatt tctccttctt ttacgtcaaa attcacgtca tacacaccaa
     3121 ccgttgcacc ggttttcttt aaaatctccg ttttcggctg gtgatcattc atcatatcta
     3181 aagcggcttg tgtcttttta ccaaatatct ttgttaaatg ttttacttct acttttacca
     3241 aacaaattct ccttttttat gtaaattcat ttttctttct gaaaaaagtg accacactat
     3301 gttaacatca tgtagtcact ttgtcaaaaa tgaaagaaat ttgcttgtcc aaactgaaaa
     3361 aaacttggct aaatctttgt aaataaaagg tttagccaag ttaatatttt tttaatattt
     3421 cacacaatca ttctgcaagc tgatgctccg ggtaaaaaaa gtttgtcata cgctgtagtg
     3481 tatattcatt tccaataaaa tgaacgatgt cccctgccgt aaatttagca taggggccag
     3541 gtgaaacaag cagttcttca ttatgagaaa ttgcgataac tgtggcgcca gtcgcctgcc
     3601 agatattcaa ctcgctaatt gaacggtcca gatgcattgc tttttctgat aaagataatt
     3661 caaacggaag taaaggattc atcttatgta ttcgcttcgt ttgattgacc agctgatcca
     3721 ataattgaga aaaatgattc agctcatctt gttgcttctc cacacttgcc atgatttctg
     3781 cttttgtatc ttggatggat tgtacatctt gatattgcaa taggaaatct tttgcttttt
     3841 ccttcgaagc aacaaatgcc ccacttccat gccgtacttc catgattcca agatctacca
     3901 gcacattgat agccttgcgg gtagtctcag gagaagcatt gtacgtactg gcaagtgttg
     3961 agcgggcatg gatcttctca cccactttat atcggttttc aacgatacgt tcggctaaat
     4021 caacagcaat ctgctggtag cgcgggcgga cgattttttt ctttccagac attttctttc
     4081 ctctttcttt ttttactttc cctcatctta ctatgctcta gcaaaataag caaaaaaggg
     4141 gagaatagcc tcttgcaacg actgatcatc gcttgatctg ttcctttttt actgggcaag
     4201 ctgtttgaca gtcatcacaa ttcttaccgg attttaaacg agtatagaca attgcgcctg
     4261 cacttccaaa gattaacaca cttaataaga atgtcgccat cctattcctc tccttctaat
     4321 gacaatatag tataaaactg gttcttggat tttggctgac ggaacaaaac ataaacgagt
     4381 cctatcatca gtaaaatagc aagatacgtc cctaacgcta cctgtccacc ctccaataag
     4441 tgtcccaact gataaatcac aaaactaact aaataagcta aaccgcattg atagcctatg
     4501 gcgatccaag tccatttctt ggcattcatt tcccgatgga tagccccaat tgccgcaaaa
     4561 caaggcgcac aaagtaaatt gaaggctaac aatgaataag ctgctgcggg agtatatgtg
     4621 tgttgtagca cttgccagat ttctctgcca ttttcggata cttccgcatg accaaacaaa
     4681 ataccaaatg tgccaataat attttcttta gcgaccaatc ctgtaatcgc tgcgactgtt
     4741 ccttgccagt ttccccaacc aagcggctga aaaacaactg ccaggattcg cccgaaaaag
     4801 gctaagatac tttggtcttc agaaacaggt tgagcataaa aattataaga agagctgaac
     4861 cagatcacga tactagaaac aaagataatc gttcctgctt ttttaacaaa agacttgctg
     4921 cggtcatagg tttgtcttag gaccgctgac cattgaggaa aatgatatgc gggcaattcc
     4981 atgataaaag gagctgtatc tccagcaaac aaacgtgttt ttttcaagct gattccagag
     5041 aaaataattg cagtcatacc aagaaaatag gcagaaggag ctacccaaga ttgatgagga
     5101 aaaaaggccc ctgctactaa ggcaataatc ggcaactttg cagaacaagg catgaaggtt
     5161 gtgaccatga tcgtcaaacg acgatcttgc tcgttttcaa tcgtgcgact ggccatgacg
     5221 cctggcactc cacatcctgt agcaatcagc ataggaatga aggattttcc tgaaagaccg
     5281 aatttcctga aaagtcgatc catgacaaaa gcgatcctcg ccatatatcc gcagtcttcc
     5341 agaaagctca agcataaaaa caatataatc aattgcggta gaaatcctaa cactgctccc
     5401 acgcctgcga tgatgccgtt caagatcagc tcctgcatcc atcccgctac ttgccattca
     5461 gctaacaatc tgccaacatg ttctggtaac catgtaccaa acaatgtgtc attgatccaa
     5521 tctgtcccca ttgttcctac tgtttgaata gccagataat agatcagcca catcacaaat
     5581 gcgaagatcg gcaaggctaa ccaacgattc gttgcaatct gatcgatttt gtcactcatc
     5641 gataattgga aagaatcatt tgacacaaca cagagtgtac atagttgggt aataaattca
     5701 taacgttcat tgaccagaat cgtctcacta tcgtcgtcca atagtttctc tgttaactgg
     5761 atcagttgct cgatctcttt ttgctgagaa actgctatct cgtaatcctc tcttaccttc
     5821 tgatctcgtt caaaatattt cattgcatac cagcgtttct tgccgctttg ttctcctagt
     5881 acatcttcaa tttcatgtaa agctgcttct aaacgtggat catatgtaca gggaacagga
     5941 atagaaggcc gcttttttgc agactcctga atcttttcga ttagtttttc gattccttgc
     6001 tttttcaata cgctgattgg taaaactggt acaccaagac cataggaaag cttttctaga
     6061 ttgatttttt ttccttcttt tttgatgaca tccatcatat tcagaccaat gatcatagga
     6121 atgtctaaat ccaataactg cgtagttaaa taaagatttc gttctaaatt ggtagcatca
     6181 ataatgttga tgatcagttc aggctgttcc tgaaccaaat aatctcttgc aatcacttct
     6241 tctggtgtat aaggagacaa agaatagatg ccgggtaggt cctgaatatg aatgttcgga
     6301 ttttttttca acgatccttc ttttttttct actgttacgc ccggccaatt ccctacatat
     6361 tgattcgaac cagtcaagag attgaacaag ctagttttcc cgctgtttgg atttccgact
     6421 aacgcgatat tcattctttt tcctcccttt gtatcaagat tagttcggcc tctgactttc
     6481 gtaacgtcag ttcataccct cgcactgtca gttcgattgg atctcccaga ggggctagtt
     6541 ttcggataac tatttttaca tttcgcgtca atcccatatc cattaaccgt cgcttcactg
     6601 cgccttgtcc gtggattgct acgacgatcc cagtctctcc tgcttttaat tgatcaagag
     6661 aagtccagct ttcagaggaa gcagtctttt tattgacaaa gatttgacct agtatttccc
     6721 gacttactgc cactcgatga ctttttacca gtaaaatggc attgttgcct gataaattga
     6781 cgacgacgat tttacttccg gcaacgatcc cgatattttg caaatggcgt tttgtttttt
     6841 cttctgtttc gattcgttcg acttgataaa cgaaattctg ctttgcctgc gctaaggtct
     6901 gcatattgat tcctctcttg tgaaataaat catgtattaa aatcattcat tatatgttct
     6961 aattttctta cgtgttacgc aaataaatga gaatcattct cacatagaat actctcggta
     7021 attgagaatg tcaattatac ttgtgatatt atgagcgaat ctttagtatt gatagataaa
     7081 aatgagaaaa aaatatttct tcatagtttt ccacaatttt ttctctatta aatttgctat
     7141 tttcccacac gatacttgtt ttcagctctt ttttcaaaaa cacaaaactt agtattccac
     7201 agtttccaca acacaatatg tagtgtttgt tgtttgactt ttttactaag agttttatac
     7261 tatctattat agataataag aatgtgttag aggaggcact caccatgaac gtaaaaatct
     7321 tttctaaaaa taattgtatg caatgtaaga tggtcaaacg gtttttaaca gaaaatcaaa
     7381 ttgcttttga agaagtcaat atcgatgaac agcctgaagc agtagactgg ttgaaagcac
     7441 aaggattcca aagtgttcct gtcattactt ctgatgcaga aacagttatc ggattccgtc
     7501 ctgatcagtt gcgtaaactt gccagctaac atgactaaaa gaatcaaaac gtgatacatg
     7561 gatttgaagg gcttgagaca gcaccagttt ttgtcgcaaa cttccaaatc cttttttaaa
     7621 gcttaaaaac aatgcaatga tttgcaatca agcatcatca aatacgaaat gaaggttggg
     7681 cgtacgtatg agtcttaaaa atttacaaga tgtcagttat ttcaagctga ataatgaaat
     7741 caatcggcca gtcaatggtc aaatcccttt gaataaggat aaagaagctt tagttgcctt
     7801 ttttgaggaa aatgtcaaac caaatacgat gacttttcca tctattatgg aaaaaatcaa
     7861 ttacttgatc gatcaagaat atatagaagc agaattcatt caattgtatt caccatcatt
     7921 tattgaaaag ctatatcaat ttttggacga gcaacatttt tctttcaaat cttttatggc
     7981 tgcttacaaa ttttattctc aatatgcgtt gaaaaccaac gacggttccg cttatctgga
     8041 aacttatgaa gatcgagtag ccttcaatgc gctttacttt gcgaacgggg atgaagaatt
     8101 agcacttgct cttgcagacg aaatgatcca tcaacgatat cagccagcta caccttcttt
     8161 cttgaatgct ggacgtaaac gtcggggcga attggtgtct tgtttcttga ctcaagtcac
     8221 tgacgatatg aactctatcg ggcgctcaat caactctgct ttgcaattgt cccgtattgg
     8281 tggcggcgta gggattactt tatcaaatct tcgtgaagcc ggcgctccta tcaaaggata
     8341 tgagggtgct gcaagtggtg tcgttccagt catgaaatta ttcgaagata gtttcagcta
     8401 ttctaatcaa ttaggtcagc gtcaaggagc tggcgtggtt tatttgaacg ttttccatcc
     8461 agacattatt gctttccttt cagcaaaaaa agaaaatgca gatgaaaaga ttcgcgtaaa
     8521 aactctttct cttggcgtga tcgtacctga taaattttat gaattgacac gtaaaaacga
     8581 agatatgtat ttatttagtc catatagtgt cgaaaaagaa tatggtgtcc cattctctta
     8641 tgtaaatatt acagctgagt atgatcatct agttgcaaat ccaaacattc gcaaacaaaa
     8701 aatcaaagcg cgtgatcttg aaaatgaaat ctcaaaatta caacaagaat ctggctatcc
     8761 ttacgtaatc aatattgata cagcgaaccg cgagaatcca atcgatggca agatcatcat
     8821 gagtaattta tgttcagaga ttttacaagt tcaaacgcct tctgtgatta acggcaaaca
     8881 ggaatatgaa gtattaggaa cagatatctc atgtaactta ggctcgacaa atatcgtgaa
     8941 tttgatgcaa agccctaact ttggtcaatc tgtgcgtacg atgactcgtg cattgacatt
     9001 tattacagat gcatctgata tcgatgtcgt accaccgatc caaaacggaa ataagctgaa
     9061 tcatacgatt ggattaggtg ccatgggctt acacacttat cttgcaaaag aacaaatgga
     9121 atatggctca gaggattcat tagactttac aaatatttac ttcatgctgt tgaattattg
     9181 gacacttgtt gaaagcaaca atatcgctcg tgaaagaaaa caagttttcc ataattttga
     9241 aaaatcagct tatgctgacg gcacttattt cgaaaaatac gtaactggag aattccagcc
     9301 aaaatcagaa aaagtcaaag cattgtttga aggcattttt atcccaactg ctgaagattg
     9361 gaatgctttg aaacaagcag taatgaaaga tggactgtat catcaaaatc gcttggcagt
     9421 agcaccaaat ggctcaattt catatatcaa cgatacgagt gcaagtattc atcctatcac
     9481 tcgcatgatc gaagaacgcc aagagaagaa aatcggtaaa atttattacc cagcgcctta
     9541 tctatccaat gagacgattc cgtattacac ttctgcttat gatatggaca tgcgtaaagt
     9601 aatcgatgtt tatgcaacgg cacaacaaca cgtcgatcaa ggcatgagtt tgactttatt
     9661 catgcgttct gaaatcccag aaggcattta tgaatggaag acaacaacga agcaaacgac
     9721 acgtgatttg aatattttaa gacattacgc tttccacaaa ggaatcaaat ccatttatta
     9781 cgtacgtacc tttactgatg atgcagaaga aatcggaagc aatcaatgtg aaagctgcgt
     9841 gatctaatat catagataga ttcgcttatt catattttaa ttaaaggagc attctcatgt
     9901 cagaaacata ttatgaagca ataaactgga atgaaattga agatatcatt gataaatcca
     9961 cttgggagaa gctgacagag caattttggc tagatacacg tatcccactt tcaaatgacc
    10021 tagatgattg gcgtacattg tctcaattgg aaaaagatac agtaggacat gtatttggcg
    10081 gtttgacact gctagatact gtccaatctg aaagtggcat ggatcagttg cgtaaagatg
    10141 tccgcactcc ccatgaagaa gccgttttga acaatattca attcatggaa tccgttcatg
    10201 ccaaaagtta ttcttctatt tttagtacac tgaatacgaa aaaagaaatc gaagaaattt
    10261 ttgactggac aaatacgaat ccttatctgc aaaaaaaagc tgagcgtatc aatgaaatct
    10321 acaaacacgg tacaccgcta gaaaagaaaa ttgccagcgt ctttttagaa acattcttgt
    10381 tctattcagg tttttatact cctctttatt atttaggaaa caacaaattg gctaacgtag
    10441 cagagatcat caaactaatc atccgagatg aatctgtaca tggaacctac atcggttaca
    10501 aattccaatt aggattcaac gagttacctg aagcagaaca agaaacgtta aaagactgga
    10561 tgtacaatct tctttatgag ctttacgaaa acgaagaacg ttatacagaa gaattatacg
    10621 atccaatcgg ctggacagaa gaagtcaaga cattcctacg ctataatgcc aacaaagcat
    10681 tgatgaacct agggatggat ccactctttc cagatactgc caatgatgtc aacccaatcg
    10741 taatgaacgg gatttcaaca ggcacaagca accatgactt cttctcacaa gtcggaaacg
    10801 gctatttgtt aggtcatgta gaagcgatga aagacgacga ttatttgatt ggattggaat
    10861 aaggatttat tttaattcaa aaatttttcg aaaaataaaa agagtttagc gtacagatat
    10921 tttaaatgta cactgaactc ttttttatgc ttattcactt gtttcttcca gcacttttgg
    10981 actgtctaac ttttccgaaa catgttccat tcttctcaat aaatagattt tccggatatt
    11041 taatattatc gtcttaatca ctgcatagac aggtatgcaa atcagcatgc ctaatattcc
    11101 tgcgacactt cctgctccaa tcaataccaa gatgatcgtc aaaggatgga tattcattga
    11161 tttcccaaat aacaacggct tgatcaaatt tccatctaat tgttggatca ctaaaatcga
    11221 caacgccata tacaaagctt gaaatggaga gataaacaaa ccgacaatga ttgctggtac
    11281 cgctccaata aaagggccga catacgggat aatattcgtg attccgcaaa tgaccgcaag
    11341 caacaagcta taaggctgat cgaatattct cattgccaaa aagctcatta caccaataat
    11401 caaagcatct aagatcgtac tgctgatata ggcagataat gtttcgttta attcttttac
    11461 cgtttggcgt aactctttac gaatcgcttt cggaaagaag tgagacaacg catcaataaa
    11521 tttgtgccca tccttgaaca taaatagcaa aataaatgga acggtaaata acaaaataaa
    11581 aaattgcatc ataaatgaaa tgatttttga gacacttgca gataaactga ctaatatgat
    11641 attgaacaaa ttactaagag ataaatttgc tgatgctaat tgttcttcca aattgaaatt
    11701 tttaaactct tcccgctgcg ccaattcatt taaccatttg cttgtttcat ctgcatacat
    11761 tggcaagctc tgggtcaatt gaattgtctg ttccattaac tgaggcacta cattcatgac
    11821 agttaaaaca actaaaatga ttacgaccaa aaacgtcaat aaaaaaccga acactctcgg
    11881 gatccgatgc ttttcgagaa aaacaacgat tggatcgaac atataataga taaaaccagc
    11941 aacaagcaat ggtaaaaaaa ttgccgaaag cattttcaat attggactaa aaatattcgg
    12001 catttgtaaa ataaagtaca cgccgatgat cgtgagtact agctccactg tccagaaaaa
    12061 taattttgat tgtttaaaac gtgattgcat acatctcctc caaggtcctt tgatacttct
    12121 attctatcat ttaacaaaga gttacgcgct gagattttca tttgcttaaa aaaaacaaaa
    12181 aaaataaaca tattttaatg agtagacgag tacttaaaca aaaacacggt ttggagtagt
    12241 gacaaaaatt atactgcagt ccctttttcc aaataaacgg tgttcaaaag cttgcatctt
    12301 cgtaataagc ccagccaaac gaaaaatata aggaactatt tttatttggc tgggcttatt
    12361 acttgatgta ggacatctat tttacaacct cagatcattt attgcgcaat actgactaaa
    12421 tgcaactttg tgatattttt gtgtacggac aactcctcga tcacatcggc ataagtgagc
    12481 ccttttggaa gatcaatcgt atagatattt ttgtacactc ggtagtcatc aacgaatttt
    12541 acgtcaaaat tcacatcttc gattacgata tttttctctt caaaataatc ggtaagaaaa
    12601 tctttggttt cttttctatg gataaattgg atttctaact ttttagtcgt aggtacatgg
    12661 atcacgcgtt tcactactgt gagtgcaata gcaatcccga taaaactgat gatcgcaatg
    12721 gaatagtaac ccatgccgat ggcgatccct aatccagcaa ttgcccataa agaagcggca
    12781 gtcgtaagtc ccgtcaccga ttgtttcgtg acgatgatcg ttcccgcgcc taagaaaccg
    12841 acaccactca ccacttgagc aatcagcctt gcttcatctg aacgaatcgt tccagcaagc
    12901 tgtggatact ctaaagcatc tcttaatgca ccactagcga tctcaacttg gatcaatgca
    12961 atgatacatg ctcccataca aactaaaata tgcgtacgca tacctgctgg acgattttta
    13021 tattgtcgtt caaaaccgat tgctccgcca aatagcatag cgatccctaa acgtaaaata
    13081 atctctggaa ttgtcaatac catatcattc atctaaacac cctccagtcc aatatatcat
    13141 gtctgaaagg agcagacaaa aaaagtgcaa tttttacctc ttacgaaaaa aggactgtga
    13201 caaaattttt gtcatagtcc tttttatgaa taaacggtgt tcaatcagct gaccctcggt
    13261 attactcaac aaacggcaaa atatggggag ctatttttcc tttgtcttct ctaatactca
    13321 ggtcaaaacg ctgatttcac aacctcttta gcctactatt gaaaattaca acttacttgc
    13381 tgcttcttca tcgatgatca caacaacatt gtcgtgattt tgcaatacac ttgctggcaa
    13441 atgattcgtt acaggtcctt caatcatccc tttgattgca tcagctttgc tttcgccaaa
    13501 tgccatcaag acgatttctt tacttttcat aattgaaccg attcccattg aaacagcttt
    13561 tgttggtaca tcttctgctt tatcgaagaa tcgtttgttt gcattgattg ttgattctgt
    13621 taagtcaact actgaagtcg ttccgtcaaa tgaagcacca ggttcattga aaccgatatg
    13681 tccattttgc ccgattccta agatttggat atcgattgga tgtgcatcga tgatttgatc
    13741 atactttttg catgctgctt ccagatcttc tgcttttcca tctggtacaa acgtttcttt
    13801 gaatggtttt ttatcaaata attggacatc catgaaatga cggtagcttt gttcatctga
    13861 accgcctagt ccaacgtact catctaagtt tacagaagtc atttcactga aatccaaatc
    13921 gcttgaagtc atttctttat ataaagtaac tggtgtgctt cctgttgcca atcctaatac
    13981 ttttgcacct ttgttcatac cttctttgat gatttcaaat gctttttttc cgccttcttc
    14041 agcgtttttt acacgaatga tttccatagt gattgctcct ttatattttg gtatagccca
    14101 attatagcat tctttttttc tttttacaat aattagtata gaccaaattg catttttttc
    14161 attttcttga caataaatag gaatccgtgt taggtttcat aagatggttc aatcaaacaa
    14221 tccatgttgc atgaaaacca agcaagtgat tcactgtgga cgctttatgt ctgcagtttt
    14281 tttatgaaaa aggagttatt atgagtatac tgacattaaa caacattacc cagcaatttg
    14341 gcgacaaaat cctctatgaa ggtgtcgatc tgcaattaaa tgctggcgaa catcttgggc
    14401 tgattggaca aaacggagcc ggtaaaagta cattgattaa aatcatcact ggtgagatct
    14461 taccagatga cggacagatc cattggcaaa aaaatattca caaaggttac ctcgatcaat
    14521 atgtggaagt cgatgaaaca ttaacaatcg aagaatttct aaaaactgcg tacgcaaaag
    14581 aatacgaaaa ggaagaaaaa attgcttttc tttaccaaac atatagcgag accttcgatg
    14641 attccttgct ggaaaaagct gggaaactcc aaacggaatt agaccaagga gtgttttatc
    14701 aaatcgacac cttagttgct gagatgagca gcgggttggg gatcgatgtc ttaggtttag
    14761 attcactgtt gaaaaatcta agtggtggac aacgttcaaa ggtgatttta gcaaaactac
    14821 ttttagaaaa cccagatgtt ctgatcttag atgaaccaac caatcactta gatgatcagc
    14881 atatccaatg gctgacgcag tttcttcagg attttgacgg tgcgtatatc gtgatttccc
    14941 atgaccaaga atttcttgat cagatcacca cccatatcgc agacatcgag tttggaaaga
    15001 ttactaagta tactggccat ctaaaacaag cattgaaaca aaaggaacaa aatcgcgaaa
    15061 gctatttgcg ccaatatcat gctcaacaaa aacaaatcga aaagaccgaa gcttatattc
    15121 gaaaatacaa agccggttcc cgatcaacga tggcaaaaag ccgacaaaaa caattagata
    15181 aaatcgaacg gctgacgcct ccagcttcag caagtaaagc tgtctttgat tttccattct
    15241 cgcctattgt cacgacatta gcagtggaaa caactgattt agtcatcgga tatgatcgac
    15301 cgctacttgc acccatcaat ttgaccatcc gctttggtga aaatattgcc attcgaggct
    15361 tcaacgggat tggaaaatct actttattaa aaacactgat cggtgagatt gaaaaaataa
    15421 gtggcgattt ccacttccca gataacacaa aaatcaatta cttttcacaa gatctgtact
    15481 gggaaaatcc attagaaaca ccactacaat atttaagcag tcaatttcca aaagcgacta
    15541 tcaaagaatt acgtcgccaa ctagctaaag cagggttagt taatcaatta gcaagtgaac
    15601 ctctcactac attaagtggt ggggaacaaa ctaaggtgaa acttgcacag ttgacaatga
    15661 accaaggtaa tttactgatt ctcgatgaac cgacgaacca tatcgatcaa gaaacgaaag
    15721 aaagcttaca agacgggata cagaaatttc caggaacagt aatcatcgtc tctcatgaac
    15781 aagaatttta tcaagatctt gtgcaacgag tcattgagat tgaaccaaaa taaacgtttg
    15841 attgaatagt tgaaaaaaag gagctgtgac aaaagttttg tcacaactcc ttcttctgaa
    15901 taaatggtgg ccaat
//
LOCUS       DBNICI010000056        15850 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.056, whole genome shotgun sequence.
ACCESSION   DBNICI010000056 DBNICI010000000
VERSION     DBNICI010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.056"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(29..2218)
                     /gene="nrdD"
                     /locus_tag="AC1ZCC_001433"
     CDS             complement(29..2218)
                     /gene="nrdD"
                     /locus_tag="AC1ZCC_001433"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HIQ1629695.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            2544..3311
                     /locus_tag="AC1ZCC_001434"
     CDS             2544..3311
                     /locus_tag="AC1ZCC_001434"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1629696.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            3301..5343
                     /locus_tag="AC1ZCC_001435"
     CDS             3301..5343
                     /locus_tag="AC1ZCC_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1629697.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            5624..5773
                     /locus_tag="AC1ZCC_001436"
     CDS             5624..5773
                     /locus_tag="AC1ZCC_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HIQ1629698.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            5784..7298
                     /gene="dltA"
                     /locus_tag="AC1ZCC_001437"
     CDS             5784..7298
                     /gene="dltA"
                     /locus_tag="AC1ZCC_001437"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HIQ1629699.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            7295..8500
                     /gene="dltB"
                     /locus_tag="AC1ZCC_001438"
     CDS             7295..8500
                     /gene="dltB"
                     /locus_tag="AC1ZCC_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HIQ1629700.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            8545..8778
                     /gene="dltC"
                     /locus_tag="AC1ZCC_001439"
     CDS             8545..8778
                     /gene="dltC"
                     /locus_tag="AC1ZCC_001439"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HIQ1629701.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            8781..10049
                     /gene="dltD"
                     /locus_tag="AC1ZCC_001440"
     CDS             8781..10049
                     /gene="dltD"
                     /locus_tag="AC1ZCC_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HIQ1629702.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            10190..11272
                     /locus_tag="AC1ZCC_001441"
     CDS             10190..11272
                     /locus_tag="AC1ZCC_001441"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIQ1629703.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            11272..12075
                     /locus_tag="AC1ZCC_001442"
     CDS             11272..12075
                     /locus_tag="AC1ZCC_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HIQ1629704.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            12145..12885
                     /locus_tag="AC1ZCC_001443"
     CDS             12145..12885
                     /locus_tag="AC1ZCC_001443"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIQ1629705.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            12896..13900
                     /locus_tag="AC1ZCC_001444"
     CDS             12896..13900
                     /locus_tag="AC1ZCC_001444"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HIQ1629706.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(13945..15789)
                     /locus_tag="AC1ZCC_001445"
     CDS             complement(13945..15789)
                     /locus_tag="AC1ZCC_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629707.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         4964 a         2668 c         3149 g         5069 t
ORIGIN      
        1 cttgatgcag gacagctttt tcacaacctc atttcatgtg tttcacacgt gaggaaattt
       61 ctttatggcg gccatgtacc atcggacgag cttgcggatt tcctaaatag ccacaagtac
      121 gtttgactac atcacatgtt tttggatcat gatttccaca ttctggacat tcgaaccctc
      181 gttctgtcgg tttgaaatca ccttcgaaac cacattgata gcaatgatcg attggcgtat
      241 tcgtaccaag atacccgatt ttgtcatatg caaaatccca tacagcttct aaagctttcg
      301 gattttgttg gagcataggg tattcacagt agtggatgaa tccacctgaa caatattttg
      361 gataatcctt ctcaaattcc aatttttcaa atggcgttgg gtttttacgc acatcatagt
      421 ggaaactgtt tgtataatat tctttatctg tgatattttc gatgatgccg aatttttctg
      481 tatccaaacg gcagaaacgg tcagttagac tttcacttgg agttgaatag acactgaaat
      541 gataaccata ttcatccccc catgcatctg cattagcttt taattctttg acgatatcaa
      601 gtgtgaaagc ttttgcttct gggtttgttt cccattcgcc accgaagaaa gctgaagcaa
      661 cttcatacaa tccaatgtag cctaacgata cagtagctcg acggtttttg aataattcat
      721 tgacagaatc ggtacgtgaa agacgtttcc cgaaagcacc atacatatat aaaattggcg
      781 catttgctgg aatggcttct ttacaacgtt ctacacgata gagaagcgca tctttcatga
      841 tttctaagcg gtcagacagt aactgccaga atttattctg atctcctttt gattcgatcg
      901 cgattcttgg caaattcaat gtaacaacac ctaaattcat tcgtccgaca tttacttctt
      961 gaccattttc gtctttccac ccttgcaaga atgaacgaca gcccataggt actttgaaac
     1021 ttccagttaa ttcaacgatt ttatcgtaat tcagaatatc aggatacatt cttttcgttg
     1081 cacattccaa tgctagttgt ttgatgtcgt aattaggatc ttcagggttt aagttcacac
     1141 cttttttcaa agtgaagatc agtttaggga agatagctgt ccgcttttca ctccctaatc
     1201 cattgattcg aatctgtaaa attgcttttt gaatttctct ttcaaaccag ttttctccta
     1261 gtccaaatcc taatgaagtg aaaggagttt gtccatttga agtaaataag gtattgattt
     1321 catattccaa actttgcata gcatcgaaga tatctttttt ggtttttgct tttgcatatt
     1381 ctttttggcg ttctggcgaa tcgatccatt cttccgcgtc ttttagatgt tttttatagt
     1441 tcaattctgc aaaaggagca agcaattcat ctgttcggtc ggcagaacag ccaccatatt
     1501 gacttgacgc tacattggca ataatctgtg agatttgtgc tgtggcggtc tggatagatt
     1561 ttggcgattc gacttcggca ttgccgattt tgaacccatt gttcagcatg cctttgaaat
     1621 caatcaagca gcaattcgtc atcggtgtat aaggatgata atccaaatca tggtaatgga
     1681 tatcgccttt ttgatgcgca tttgctatat gtggtggcaa cattttcagt ccgattgatt
     1741 ttccaacgat cccggctgtc agatctcgct gtgtattgaa aacattactg tctttatttg
     1801 cgttttcgtt cacgacagtt tggtccttgt tgattagctt ttcaatcgaa aagttgatat
     1861 ctgttgcttt actgcgagca aaatctcgtt tggtacgata attgatgtac tcttctgcta
     1921 attcatattc gtttttttct agtaatatat gttccacgat gttttgaatc tcatagattt
     1981 tgacattttc agcaaaccgg cttttgattt cttggacaac caagtctgca atcatttgaa
     2041 ttttttcata agtaagtggt gataatgagc cgtgaatttt cttttctgct ttgatcagtg
     2101 cttcattgat cttttgttca tcgaaatcaa catggcggcc atcgcgtttg atgactttca
     2161 tctgggatgt ctctggctgc tgagtaacga cagttttttc ttcataattt actttcatta
     2221 tcttcaactc ctgctaaatt gttcttgtct atcataaaac aaaatcgggg aaggagcaac
     2281 taaatatcgg ctatatatag tattcaatgt gctgtataat tggatttcta tactatatgt
     2341 agtgtatatt ttattgtgaa aaaaggtgaa aacttgtagc tggtggattt gttttttgca
     2401 tatttaattt tgctgaataa aataattgaa taaaattttt tattcagtcc aagtaaaagt
     2461 gacaaaaaca taagagcaaa ttattcgtag atatgctact atgcaaataa atagaaatga
     2521 aaaatgaaag aggaggagaa gaattggaaa agatgatcga agtgaaacat ctaagtaaaa
     2581 gctacggcag caaactttct cccgtcaaag ccttagataa tatctcattc acagtagaag
     2641 aaggcgagtt tgttgggatt atgggcccaa gtggtgcagg aaagacaacg ctgatgaata
     2701 tcttgtccac gatcaatttg cctacgatgg gcagtgtctc gatccaagga aaagaaatca
     2761 caaaaatgaa aaacagtgag ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc
     2821 aagagttcaa tttgatcgat accctaaatg caaaagacaa tatcctattg cccctagcag
     2881 ttgaacggat gactaaagag gaaatggaaa agagagtaag acatgtagct caattgttga
     2941 atatagaaga actgctcaaa cggtatcccg atgaactttc tgtcggacag agacaacgaa
     3001 ttgcagctgc tagagcactt gttgttcagc cacaagtgat ttttgcagat gaaccaactg
     3061 gctctttgga ctcgaagtcg gctacagaat tgttgaacta cttgaagacc atgaatcaaa
     3121 aagaaaaagc aacgatactg ttagtcaccc atgatccata tacagcaagc tactgcgata
     3181 gaattttatt tattaaagat ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa
     3241 gagagttctt tgagaaagta atagacatgc aggcgacgat tggcggagga ggaaaaatga
     3301 atgctgtata aactagccgt taaaagtttt tttcggcagt cacgaggcta tcttgtttat
     3361 tttttcagtt tgactttatc cgtaatgatc tattattcct tcagcgcaat gacctacgat
     3421 caatctttag tcagaagagc aagtcaagat acttcgatag atggtggatt gagttttggt
     3481 ggatttatca ttactgttgt tttgcttttt ttcgtattca gtgccaaccg atttttcttg
     3541 aatagaaggc aaaaggaaat aggcatctat cagctttttg gtatgaataa actgcagata
     3601 tcaggtatat atgtgttaga aatcatgata attggattgt tcgcctgtat ttctgggata
     3661 ttactcggta ttattttttc caaactcttt tcaatgattt tggttcgaat gatggatatg
     3721 gatcttacta gtccattttt tatctccatt ccatcagtag tagatacttt atttgctttt
     3781 ttcatcatcc ttttagcagt atctgtatat tcaatatgga aaatatggcg ctatccaatg
     3841 attcgaagtt ttggagaaaa agaacaagca gatagttcaa caatgcgctt caggacaagg
     3901 caccgattac tcgcagtcat cgggttacta cttttaacga gcggatattt tggagctagt
     3961 cattttaggg aaatcacttc atggatgatc agttatcatt atcttgatac attagttttc
     4021 ttttttcctt ttttaatttt tttcgtttgt gtggtcggta cttatttgtt tttctgttat
     4081 acatttcggt tatttttgat catattgagt aaatggaaga tttattacta tggtatcaat
     4141 tttttgataa tgggtaacac gcaagttcat ttgctcaaaa gttggcgtac aaattctctg
     4201 attacagtca ttctcggaat ttctttaatg acaattggcg gcacggctag tatctctact
     4261 attttatcct accaggagaa agtttcctcc ccgatggact atcagtttga tgttgagaca
     4321 gagaaaaaaa tcaagccgat tctagcagag gaagggcaga agataactaa agaaatcacc
     4381 ttgcattata aagtcattgg gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa
     4441 actgctaatc tgatcacgga aacacaatat caagccttca gaaaagttaa tccagatttg
     4501 ccgaacattt ctttgaaatc acctggtcat actgttttgc ttgatcaaat gcaaactctt
     4561 tttcggaata tttcattata tggatctagt attggactgc cagataatca agaattgaag
     4621 attcaacaaa tgcttccaag tgtattaggt gacgaaacaa tggtatatct aagtcctgta
     4681 ctgattgtag atgataccgt ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat
     4741 gtagatgtat caggtggaaa taatgaaaaa atagatgaac gactgagcaa tgacctgtcg
     4801 ataaagtggc tcaatgctgt ttattattct tatgatatcg taaatggaag tctacaagga
     4861 gaaattagca caaagaaact tccggatgac caaatgaagg aagcacaatt tagccaagaa
     4921 agctctcgtt tgaatttctc ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc
     4981 ggtatcaata tttttgtcac actatttgtc gggatgatcg tgattattgc tactggtagt
     5041 attttgacag tccggcaatt agcagaagca gaggaagaaa aagaaaatta tcacctattg
     5101 actaagctgg ggattccaca aagaaggatc agacggatga tcttcgagca aaacgcactt
     5161 accttctttc cacccatgat attagggatc actcacgcag tatttgcaat caatgtcttc
     5221 actcagtata taaaaactgc cgattactgg ttggcttatc ttgtgagtgg cttattgatc
     5281 cttatctatc ttttgcttta tgtcattacg agtcagttat attgtcggat tatcgaggaa
     5341 tgatatacgg aataagacaa gataataaat agaactaaat tttgaatacc atttatttgg
     5401 aataagaggt tgtgacacga tttttgtcac aacctcttta ttttttatat tttttaaaag
     5461 tacgagaaat tataaagaaa aaagcagtga tttgatgtgc tatttttata aaagctatgt
     5521 gacaaaactg ttagtaaaaa aacggtttct gtaagctaga cacatgtttc tttttgtcaa
     5581 aattagttac actttgtgtg aggttatatg aggagggaat ccaatgaaca aatggcgttc
     5641 taatccaaaa ttgaattatt ggggaaaatt catcattcga acaatttttt atagtgcagt
     5701 actgctgttc ctgatttatc tgtatcacta taaaaatatt caaggaggaa cttttatcta
     5761 taacgaattt taggaggaag agtatggaaa tcaaaacgat tattgaagca atcgatgatt
     5821 ggggaataaa tgaacctgat cgagttgctt atcaggcgga tgagacgcat acttttggtg
     5881 aattgaaaca agcatcagac gcacttgctt attatttaga aaaaaaggta gaaggcgatg
     5941 gaccaattgt cgtttttggc aatttggagt ttgaaatgat tgtctcattt ctaggggttg
     6001 tcaaatctgg ccatgcctat ttgccgattg aagagcatac acccaaggag cgtatcctat
     6061 cgatctttcg ggtggctaaa ccatctatgg tcatcagtat cggggactgg atagaagata
     6121 ttccttcggt tcctgtcata acaaaaaaag aatttcaaga aatcaagaag ataccagtag
     6181 gattttatcc cagaaatagc gtaaaaggta cagaaaattt ttatattatt tttacttctg
     6241 gtactactgg agaaccaaaa ggcgtacaaa tcagtcatga taatctagtc agctttgttc
     6301 aatggacgat ggaagacttt gggatcaagc ctggtatgca tttcttagcc caagcgccat
     6361 tttcttttga tttatcggta ttttctattt atcctgcatt agtttctggc ggaatgctga
     6421 agccgttaaa taaagcagtt gttcaagact ttcgtcaatt attcgcgaca ttgccagcct
     6481 tgaagttgaa tgtgtgggtt tctactccat catttatgga tatttgcttg atggaaccta
     6541 cattcaatgg agaaaatgtg ccggaattgg acatgttttt attttgcgga gaagaattga
     6601 cgaaaaaaac tgcagaaagt ctacttgatc gattcccaaa tgcacggata tataatactt
     6661 acggtccaac agaagcaact gtagctattt caagtattca gattgaccag catgtactag
     6721 atagttatga ccgactaccg attggctacg taaaaaaaga cacgaaagtt tctattgtcc
     6781 aagaaggtaa acctgtttca aaaggagaaa cgggtgaaat cattattgca ggaccaagcg
     6841 tatcaaaagg ttacctgaac aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg
     6901 acttagcaag ttaccataca ggggatgcag gtagactaga tgaatatggg cttttatttt
     6961 atgaaggacg tatggatcag caggtcaaat tacatggata ccggattgaa ctcggggata
     7021 tcgaacatta tctgctccaa gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc
     7081 aaggaacaaa agtacagcag ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc
     7141 cggattttca gttgactaaa tctatcaaag aacaattact agaagttgta atggattata
     7201 tgattcctca gaagttccat tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg
     7261 atcgaaaaaa attaacagct gaggtgaatc ctgaatgatg ttacctcata tgatccctta
     7321 tgcagagccg ttttattttt ttcttttagc cattgcgctt gtaccgatcg ttctatcttt
     7381 attgatatgg gaaaaaagat taccagttta tcaatcagca gtgacacttt tctttttatt
     7441 tgtcagcttt ggaggtaatt attggcaaca gggagcggcg ctgattgggt atgttttgtg
     7501 gcagacgata cttgtatggg gctattttgt ttatcggcaa aggaaaaatc agacaggatg
     7561 gttttttgcc gccgttattt tagcgataat tccactgttt ttaacgaaag tgactccttt
     7621 ttttgaacat ggaaaagcat cgttacttgg ttttttagga atctcttatt taacctttaa
     7681 atctgtacaa atgatcatgg aaataagaga tggcatgatc aaaacatata agccatttcg
     7741 ttatatccag tttctgctgt tctttccaac aatctcttct ggaccaatcg atcgctatag
     7801 gagatttgaa aaagatcagc tccatccgcc tgttcgtgat aaatatgttg atcttttggg
     7861 aaaaggcgtc caaaatattt ttgtcggttt tttatataaa ttcattatcg gttattattt
     7921 tggtcaagta ctgcttcctg tagtgggacg cattgcctta cagcatggtg gtatttcatg
     7981 ggcattagta ggttatatgt acgtgtacag tatgtatttg ttttttgatt ttgctggcta
     8041 tagcttattt gcagtcggta caagctacat gatgggatat cagacaccaa tcaatttcaa
     8101 taaacctttt ttatcatgga atatcaaaga attctggaat cgttggcata tgacgctgtc
     8161 cttttggttc cgtgattatg tatatatgcg attgatgttc ttcatgatga agaaaaaatt
     8221 ttttaaaagc aagatcgtaa tgtcaaatat cggttatttt gctttgtttc ttctaatggg
     8281 tatatggcat ggattgacgt ggtactacat cgtatatgga ctgtatcatg cttgtctgat
     8341 ctgtctgaca gatgcatgga tccgatttaa gaagaagcat aaagagcagt tgccttctaa
     8401 caaatttacg catggcttag ccgtattttt gacatttcaa gcagtatgtt ttagcttcct
     8461 gattttttca ggatttttag ataaactttt ttttagctaa cgaatagtga catattaatt
     8521 tatataacaa aggagaaaag gaaaatggaa gaacaaattt tatcaatctt agaagaaata
     8581 acaggaacag atgaagtaag aaaaaatcgt gatattgatc tatttgatga agggttgctt
     8641 gattctttag catctgttca attattagtc gagctagacg gagagctgga tatccaagtc
     8701 cctgtatctg aattcgagcg agaggattgg tgcacaccga ataaaatcat tcaacaagca
     8761 actgcattga agggttgagt atgaaaaaga aactttttgc tattttcggc ccggttgttt
     8821 tagctgttgg cttactgttt gtgtttttta cctcctccta tcaagtagat atctctaatc
     8881 cagaaacgat tagacgtgcc tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga
     8941 taaaaaatta tgcattatct gaaaaacaat atattccttt ttttggctca agtgaattaa
     9001 gcagaatcag tccatttcat ccgtctgttc tggcagaaaa ataccagcga aactatcgtc
     9061 catttttgct gggagcaccc gggacacaat cgttaagcca atatatgatg atgcgttctg
     9121 caggagatgc tatgaaaaac aagaaagtcg tctttattat ttctcctcag tggttcgtta
     9181 aaaatggtgt aaaaactgat tactttaata cgtattattc tgaacttcaa acatacgact
     9241 ggttgttttc aatgaagaaa gtaacgcctg ctgatcgcta tttagctaga agattattaa
     9301 ctttctcaaa agtaaaagag aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag
     9361 ggaagctacc tttacctgaa tctttgaatc agctgcgcag ccaatggaac atgctgaaga
     9421 gagaggatga agtattcagc aatattgggt tgaaagatcg acagcaaaaa atcgatcatg
     9481 agtcaaaacg attaccaaaa caataccaag aaacagaatt atccatttta gcaaatcaaa
     9541 ttggagaacg agaaacgacg aataatccat tcgggttgaa aaacgatttt tatacgcatc
     9601 gtatccgagc ccacgagcca gagctcaaac agtcacaaaa aaattgggat tatcgttttt
     9661 cgccagaatt ttctgatttt cagctggtct tggatcaatt agcaaaaaac cataacgaag
     9721 tattgttcat catcccaccg gttaacgaga aatggagcga ttataccgga ctatctcaag
     9781 aaatgttaca aggatttgcc aaaaagatca aatttcaatt aaatagccaa ggattcaatc
     9841 gaatagctga ttttgtcaat caagcgggta ccaactattt tatggaagac acgatccatc
     9901 taggctggaa aggctggttg gctgcagatc aacagatcag accgttttta gaagaaaatc
     9961 atattactgc tagtaagtat catcttgacg atgctttttt tagcaaatct tggcaacatc
    10021 aaataccaga taaactacaa ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt
    10081 ctttattttt gaatattctg aataaaagat aaaaatacca agtttgcctg aacaaaaatc
    10141 ataagcaatg gtataatcac aatatgaata tagaaatggg ggaaaacatt tggatacaaa
    10201 agaagaacag ctacttattg atttaacttc tgcaaaagga gtacctggaa atgaagaaga
    10261 ggtaagagaa gtattcagag aatatgccaa accattcgct gatgatattt tttatgacgg
    10321 tttaggcagt gtgattgcca aacatggtgc tggcggaggc cccaaggtct ttatttctgg
    10381 acatatggac gaagttggtt ttatggtaac aaaaataacc gaaaaaggat tcttggaatt
    10441 ccaaactctt ggaggttggt gggggcaagt catgcttgct cagcaagttg aaatcaaaac
    10501 acgtgaaggg aaaatcgtcc acggtgtgat tggttccaaa ccaccacatg ttttaacacc
    10561 tcaagtaaga aataaacctt atgaaataaa ggatatgttc attgatattg gcgcatcaag
    10621 ccaagaagaa gccaaagagt ggggcatacg tcctggagat atggtcactc catatattga
    10681 atacaaacga atgaatggca gtaaatattt actggcaaaa gcatgggata atcgtatcgg
    10741 gacagctgtt tctctgcgag tattagagaa tctatctaaa gaagcacacc caaatgtttt
    10801 attcgcagga agcgatgtgc aagaagaagt aggcttgcga ggtgctcgaa caagtacaca
    10861 ccttgtaaat cccgatattg cttttgcttt agatactggt acagcaggag acactccagg
    10921 aatgacacca aaagaagcag attcgatttt aggaaaaggc ccacagattt tgatttttga
    10981 tgcatctatg atcccgcata aaaaattatt gaattttgtt attgatgtag cagaggaaat
    11041 ggatattcca ttccaatata cagtgatttc tggcggagga actgatgcag gacaaatgca
    11101 tttgacaaga gatggtgttc cgtctgtagc cattaccgtt cctgttcgtt atctgcattc
    11161 ccatacatca gtgattcatg aagatgatta tttgaataca gtgaaactag tcacagaatt
    11221 agtgaaacgg cttgatagtg aaacagttga aaaaattcgg agttatgaat aatgaataat
    11281 agaataccta tttctgaata tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga
    11341 atcaatactt ggcttgtttt cttatctata atatttacga gcgtcatcca gaactttttt
    11401 gacggtttca gtccttttcc ggaagaaagc atgcagcagc acttattgag ttttctagtc
    11461 gataatttac ttttattcgc attcaactat gcattgtttt acattgcgct agtagttgta
    11521 cggggtggca gaccaaaaag taacctttta ttttcagttt tccaaagaaa gtattttggt
    11581 tcgatacttg gcatcaattt gatcaattca gtcatcaact ggctactcaa cagtttaatc
    11641 tttctgccca tgtttttaat cagcggagtg agtacttata tgcagctgct tttcagttca
    11701 aatgcggtac ctacgggtgt catgccagaa gattttcttg atatcagctt tgttttatcg
    11761 atgataggga tgatttttgt ttcagcactc atcatgagta ttgttggcgg tttattccaa
    11821 tttgctgcat ggacaaagtt cgactatcct gatttgtcgg ttattcaatg tttgaaatat
    11881 ggctggtttt tattgaaaga tagaattggt acgtatattt tgttgcagct gtcttttatc
    11941 ggctggtatt tgctaggagc aatcgctttg ttcatcggtc ttttgtgggt cattgtttat
    12001 gtgaatgtgt cagttgcaga attttatgaa caagcacgga ttgaaaaagg aggtccagac
    12061 gcctatttta cttagcatgt aagcagttta ttcatcaaaa aaataacttt tgtggtagga
    12121 taaatctttg agggggaatg tacgatgaaa ttaactgttt taggatgctt aggagcgtat
    12181 ccttataaaa atcaagggac aacaggttat ttgcttcaat ctgacgggtt caatttgttg
    12241 atcgatgcag gtagtgctac gctgatcaaa ttacaagaac accttgatcc acttgatcta
    12301 gatgctgtga tccttagcca ttatcatcat gatcatattg ctgaccttgg tgtattacag
    12361 tattactggc aacttcatcc taagagaaat ccggagacat tgctttcaat ttatggacac
    12421 acaaaagatt ccattcactt tgaagaattg acgttggaaa atgtctcaaa aggacatcct
    12481 tattttgaag ctgaagaatt aaaaattggt ccttttctga ttacttttat ggaaacaatc
    12541 catccagtag tatgttacgc catgcgtatc gtagaagaaa caacggggaa agtattcgta
    12601 tttacaggtg attcaggtta tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg
    12661 tttctagcag atacctatct ttttgaagga aatgaacatc atcatgccca tttcacttca
    12721 aaagaagctg gagaacttgc gaaatcagct aacgtaaaga aacttgtact tacacaccta
    12781 cctcagtttg gtgatttaga acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg
    12841 attgatatag aacttgctgc agttgacaaa gtgtttaaca tttaggagga agaacatgat
    12901 ttttgtacca aatgaaaacc acgacccaag aatcaattta gcaattgaac aatatttgct
    12961 gcaagaaatg cctttagatg aacctatttt gctcttttat atcaatgaac cttcaatcat
    13021 catcggcaga aaccaaaata cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg
    13081 cattcatgtt gtccgtcgct taagtggagg cggagctgtc taccatgatt ttggaaacct
    13141 gaactttagt tttatcatgc ctgatgatgg ggattcattc cgtgactttg cgaaagtaac
    13201 acagccaatc atccaagcac ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg
    13261 aaatgatcta gtcattgatg gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg
    13321 tcggatgttt gctcatggaa caattatgtt cgactctgat atcaatgaag tagtcaatgc
    13381 attaaaagta aaaaaagaca aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt
    13441 aacgaatatc aaaccatttc ttccagaaga aaagcaaaaa atgacaacag aagaatttcg
    13501 tcaagaaatc ttgttgaaaa ttttcggcgt agagtcagta gaagaagtaa aaacgtacga
    13561 attgactgat gaagattgga aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg
    13621 ggattggaat tacgggaagt ctccagattt caatttctct cgccaaaaac gtttttctat
    13681 cggttcgatt gaagtccatt tgaatgtctc agaaggaatg atcaaagaag caaaaatctt
    13741 tggcgatttc ttcggactag gagatatctc tgacgtggaa aaaaaattgg ttggacagaa
    13801 atatgataaa gcatctctat caaaaatagt cagccaaatt gatgtgaaga aatattttgg
    13861 tgcgatagaa ccagaagaat tgcttacctt actttattga aaataaaaaa tagcggaatc
    13921 cacatatatg gattccgcta ttttttatac aggattcggg taatttcgag agagttgaac
    13981 agtgatccga tcaatgaaag tatctttttc ctgcgttagt gttcctgtgt aacgatccgc
    14041 tacgtctttc atcatcatta agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc
    14101 tcgactaaat aggtttgtat cgtaatgtgc actatgttcg caacaaatga cgaaatgttc
    14161 ctcttgttcg tgtatcgaca aataaatttt tcgatacagt ggattttcga tggcatgagt
    14221 ttctctaaag gaatgctcta aagcgtggat caaaagttga gccagatcgt catcgttgat
    14281 aggaagttct tcgggaagat ctgcttggaa ggttatctga atattccgtt tggcagctaa
    14341 cttctggtaa cgcgctaaga taagatttgt cacctggtgg ttgcaaaaag aacacagcat
    14401 ttcgaaattt tttgcttctt ctaatatctt ttccacatag tttttagctt ttgtttggtc
    14461 cgtatgcatt aaatcttgca gattctccat ctcttggaca aattccaggc gaagtgtatt
    14521 tacttcttgg atatgctgtc gaagctgctc ataatgttgc tccaacaaat catttttgat
    14581 ttttaagaaa tctacttgtc tccgatcttt gtcggtattt aagatgtgat cgattagatt
    14641 gtagctgata ataacgatta gaatgataat atataaaatc gtagaccaat tgatcgttgt
    14701 ttgatagata cctaatgccg tttggatgta aatcaagcaa tggataatgg ctaccaagac
    14761 gatccaaggt gtacaagcta cataaaaacg gttattctta tatgcttcgc caatgcatgc
    14821 aagtgaagtg actaaagtgc ttataacact agcaatatta gcaatcggca ttaattcagg
    14881 aagttctgca ggagtaaaag ttgcataaag cagaacgccg ataaaaatca aaagttggaa
    14941 cgtacaaaaa attccgtacc attttttata gtaaaacatt cggctccaat agtagcagat
    15001 gatgaaagct gacgttaaga taataaataa atgagataaa aaagaatgaa cgaatggttc
    15061 aaaaagaata ccacttaaaa tatcttctga tacagcttca aatcctaatg ccaaagcaaa
    15121 gcaacttaag agaataagtg agtattcaag acgtttttgt ttatacatca agtagatcgt
    15181 atagaaaaag gtccctatag cgataaaaac agcgattatc atcgtcaata cttgcggcac
    15241 tgattgtgaa taaataaaac taatcaatgg tccagttgtt cctagataaa ctttcggtgg
    15301 cagtccagca tagttctgat aaggagatga gactgtaagg gtcaattttt ttccgatata
    15361 atttgctggt aaatctatca cagccaatga gagtccggga ttgctttctt cctgataatc
    15421 tttccgttgg tataaaatct tttcgcctag atatacagta atccattgat ggtttccttt
    15481 gattagtaaa tttgcttcgt ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc
    15541 gtctttatcc acggtaggaa tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc
    15601 atcttttgta tagaaaaccc acgaatcttt tatttctttc atttgattgc tgtccgcagg
    15661 agaaaaaata aaataaaaga aataagcaat tcctgttaaa aataacaatg ataatataat
    15721 gattatgctc cactttatac gtaaataaaa agattctttt ttattatata tgttattaaa
    15781 aaaagacata aaggtattcc tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac
    15841 taacgtcaat
//
LOCUS       DBNICI010000057        15630 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.057, whole genome shotgun sequence.
ACCESSION   DBNICI010000057 DBNICI010000000
VERSION     DBNICI010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.057"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            23..646
                     /locus_tag="AC1ZCC_001446"
     CDS             23..646
                     /locus_tag="AC1ZCC_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629708.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /gene="trpX"
                     /locus_tag="AC1ZCC_001447"
     CDS             1096..2145
                     /gene="trpX"
                     /locus_tag="AC1ZCC_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HIQ1629709.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="AC1ZCC_001448"
     CDS             2142..3023
                     /locus_tag="AC1ZCC_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1629710.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="AC1ZCC_001449"
     CDS             3026..3796
                     /locus_tag="AC1ZCC_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1629711.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="AC1ZCC_001450"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="AC1ZCC_001450"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIQ1629712.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="AC1ZCC_001451"
     CDS             5107..6270
                     /locus_tag="AC1ZCC_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIQ1629713.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="AC1ZCC_001452"
     CDS             6387..6797
                     /locus_tag="AC1ZCC_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIQ1629714.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="AC1ZCC_001453"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="AC1ZCC_001453"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HIQ1629715.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="AC1ZCC_001454"
     CDS             9370..9654
                     /locus_tag="AC1ZCC_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629716.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9814..10050
                     /locus_tag="AC1ZCC_001455"
     CDS             9814..10050
                     /locus_tag="AC1ZCC_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIQ1629717.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="AC1ZCC_001456"
     CDS             10047..10445
                     /locus_tag="AC1ZCC_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HIQ1629718.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11112..12056)
                     /locus_tag="AC1ZCC_001457"
     CDS             complement(11112..12056)
                     /locus_tag="AC1ZCC_001457"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HIQ1629719.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12352..13296)
                     /locus_tag="AC1ZCC_001458"
     CDS             complement(12352..13296)
                     /locus_tag="AC1ZCC_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIQ1629720.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13517..14731
                     /locus_tag="AC1ZCC_001459"
     CDS             13517..14731
                     /locus_tag="AC1ZCC_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIQ1629721.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14753..>15630
                     /locus_tag="AC1ZCC_001460"
     CDS             14753..>15630
                     /locus_tag="AC1ZCC_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-rhamnosidase"
                     /protein_id="HIQ1629722.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWI"
BASE COUNT         5193 a         2689 c         3122 g         4626 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata ttatacaaag
    10681 tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc gtcatttttc
    10741 catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt actttttttg
    10801 tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata tgaacgaatt
    10861 agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca cagataaaca
    10921 ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca tatgtacgga
    10981 tacattttcg gaccgattaa ttttaaccat tctatttatt tgcttaccta ttagatatca
    11041 ataaaaaact gcttaacagc tatttcagct gttaagcagt tttaggattt ttagttcata
    11101 ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt tgatttgctg
    11161 aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac accattttcg
    11221 tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc agttaaataa
    11281 gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat acgtgcagta
    11341 ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata agcagcatta
    11401 cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc tttttctaca
    11461 atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac ttctgaatca
    11521 ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa tttcaatgcg
    11581 atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac acgtgatgtt
    11641 ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt tgaagcaaca
    11701 acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg catgattgac
    11761 gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat accagctgtg
    11821 ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac agccacattt
    11881 tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc tttttcttga
    11941 ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc ataagcgata
    12001 cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt tttcatgaat
    12061 aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct tgtttattca
    12121 ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa acgaattcct
    12181 gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat aatacaacca
    12241 aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa taagacagca
    12301 atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt cttaatgttt
    12361 atttctccat tcattgggag tacatgttga atacttcttg aataatttga ataaagaagt
    12421 cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg tttcttgaat
    12481 cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg aagcatcaaa
    12541 atacattttc aatttattac taagatagac cggattgtag ccaaaatgag ctgctgtttc
    12601 ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa gcatctcatt
    12661 ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag tcaattcgat
    12721 cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg tatcaaaata
    12781 aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc cgcgaaaatg
    12841 aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag aagaaagtag
    12901 attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt cttttaataa
    12961 gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag cttctttatc
    13021 aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa tttgtaaaca
    13081 tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg gaacttccgc
    13141 atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc ctcggtggat
    13201 ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca ttccttgttc
    13261 acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat tcagctagtt
    13321 ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca cgctgtactg
    13381 ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg gacatgaact
    13441 tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga taagcgctta
    13501 caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc tgctgtcagt
    13561 ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta tgtcagctac
    13621 ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct cttttatggt
    13681 cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa aacaaactgt
    13741 gatcatcgga ttagtgattg catcgatttg tggtatcctc cccctcttct cacaaagtta
    13801 cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga tcaattcttt
    13861 ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta tgattggttt
    13921 tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgctg gacagctagt
    13981 acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta ttcttgtctt
    14041 gtttgttttg tttgttccta cacctgaaac aactgaactt caatcgcatg gcacagcccc
    14101 aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt ttctcttgct
    14161 ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga cgacttattc
    14221 tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg caagtatggc
    14281 tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc ttacactttc
    14341 tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc tgtatgtgat
    14401 tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt acttattcaa
    14461 ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc aatacttcaa tcttattggt
    14521 tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct cttttgcttc
    14581 tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt tgattagcgc
    14641 gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa gagtagatga
    14701 agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta aaatgacaaa
    14761 tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc gccatggact
    14821 gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact ggtacatgga
    14881 tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac cttccatgtt
    14941 taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac atccatgtgg
    15001 aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag gacatacatc
    15061 agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg gctggacagc
    15121 aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg atatcgcaaa
    15181 agatccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg aaacggagat
    15241 tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag aactaatatc
    15301 tctggatgac catatcgaat ctatcactct atgttatgga gagtccgatg tagaagctat
    15361 ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga aagctcgtaa
    15421 acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc aacttcaagt
    15481 ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt cttctttact
    15541 gaatcagatc tggtcagtat cagaagaagc cttcaagtta tgtagtgatt tgttttttat
    15601 cgacggtatc aaacgagatc gctggatttg
//
LOCUS       DBNICI010000058        15367 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.058, whole genome shotgun sequence.
ACCESSION   DBNICI010000058 DBNICI010000000
VERSION     DBNICI010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.058"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(12..482)
                     /locus_tag="AC1ZCC_001461"
     CDS             complement(12..482)
                     /locus_tag="AC1ZCC_001461"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HIQ1629723.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="AC1ZCC_001462"
     CDS             complement(641..1099)
                     /locus_tag="AC1ZCC_001462"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HIQ1629724.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2414)
                     /locus_tag="AC1ZCC_001463"
                     /pseudo
     CDS             complement(1270..2414)
                     /locus_tag="AC1ZCC_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            complement(2427..3344)
                     /locus_tag="AC1ZCC_001464"
     CDS             complement(2427..3344)
                     /locus_tag="AC1ZCC_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIQ1629725.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3772..4335
                     /gene="efp"
                     /locus_tag="AC1ZCC_001465"
     CDS             3772..4335
                     /gene="efp"
                     /locus_tag="AC1ZCC_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIQ1629726.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4436..4606
                     /locus_tag="AC1ZCC_001466"
     CDS             4436..4606
                     /locus_tag="AC1ZCC_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629727.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            4821..6080
                     /gene="lysA"
                     /locus_tag="AC1ZCC_001467"
     CDS             4821..6080
                     /gene="lysA"
                     /locus_tag="AC1ZCC_001467"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIQ1629728.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6067..6867
                     /gene="dapF"
                     /locus_tag="AC1ZCC_001468"
     CDS             6067..6867
                     /gene="dapF"
                     /locus_tag="AC1ZCC_001468"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIQ1629729.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6870..7751
                     /gene="dapA"
                     /locus_tag="AC1ZCC_001469"
     CDS             6870..7751
                     /gene="dapA"
                     /locus_tag="AC1ZCC_001469"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIQ1629730.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7748..8464
                     /gene="dapB"
                     /locus_tag="AC1ZCC_001470"
     CDS             7748..8464
                     /gene="dapB"
                     /locus_tag="AC1ZCC_001470"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIQ1629731.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8470..9165
                     /gene="dapD"
                     /locus_tag="AC1ZCC_001471"
     CDS             8470..9165
                     /gene="dapD"
                     /locus_tag="AC1ZCC_001471"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HIQ1629732.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9181..10182
                     /locus_tag="AC1ZCC_001472"
     CDS             9181..10182
                     /locus_tag="AC1ZCC_001472"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIQ1629733.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10191..11540
                     /locus_tag="AC1ZCC_001473"
     CDS             10191..11540
                     /locus_tag="AC1ZCC_001473"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIQ1629734.1"
                     /translation="MKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGKRTSD
                     DDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQKQLED
                     EKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITKAFKR
                     YPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMADPRI
                     IDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGTKISN
                     HEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPSGIDN
                     IGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSGKVLS
                     ILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTPSNS"
     gene            11500..12651
                     /locus_tag="AC1ZCC_001474"
     CDS             11500..12651
                     /locus_tag="AC1ZCC_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIQ1629735.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12626..13795
                     /locus_tag="AC1ZCC_001475"
     CDS             12626..13795
                     /locus_tag="AC1ZCC_001475"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIQ1629736.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            13970..15349
                     /locus_tag="AC1ZCC_001476"
     CDS             13970..15349
                     /locus_tag="AC1ZCC_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIQ1629737.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         5010 a         2717 c         3275 g         4365 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatctttt ggttagaagt cactaaacca
     1801 gctactacat cgctgtgtcc gcctagatat ttggtaccgc tatggacaac gatatcagaa
     1861 ccgagatcta aaggtctttg aaaataagga gtagcaaatg tattgtcagc aataactatc
     1921 aatccatgtt gatgagccag agtggcactt ttttctaaat cagtaatctt tagaagcgga
     1981 ttgctaggtg tttcaagata aagtgctttt gtattgggtt tgatggattg ttctatttta
     2041 tcaagattac tcgtatctat gatcgtgtac tcgagaccat ttttagtaag aactttgtca
     2101 aatagacgga atgttcctcc atagacatca tctcctaata agatatgatc gccagcttga
     2161 aaaagagaaa aaacggcatg aatcccagaa agaccagaag aaaaagcgaa tcctcgaaca
     2221 ccgccttcca aatcagcaat cagttcttcc aatgcaaaac gagtaggatt accagatctt
     2281 gaatattcat attgtttagg ctggccgacg ccatcttgcc gataagtgga tgtttgataa
     2341 ataggtacac tgacagctcc tgttgtggga tcttcactga tgcctccgtg gatcaatttt
     2401 gtttgaatgt gcatgtttct ttcctcctat aagtaaatgt tttttgataa atagcggtct
     2461 gctgcgtctg gaaaaatcgt aacgacacga tgaccaggtg gtaaacgacg aatttctctt
     2521 aatgctgctg caaaagccgc accactcgaa cttccaacta atagtccttg ttctcgtgct
     2581 aactgcctag tatagttaaa cccttctaca tcagagattg tctcgatctg attgattgaa
     2641 aggggagaaa gaaacggcgg aatgaattct acaccgatac cctcaatttc gtgaggggct
     2701 ggatcaccac catttaacac agagccctct ggttctacac cgattattcg aatgtcggga
     2761 tatttttctt tcaaaaaggt agaagttcca gcaaaggttc caccactccc aattcctgct
     2821 acaaaactat ggatattttc tttgatttca tgaaagattt ctggccctaa cgtatgatag
     2881 taagcagcag gattatcttt attttcaaat tgaagaggca gatagctgtt tgatatctcc
     2941 tctgcaagtt ttttactttt gctgatggca ccgatgattc cttgctcact aggagaatgg
     3001 atgatctttg ctcccaatgc tttcatcaat tgttgttttt ctaagctaaa cttctctgga
     3061 acaacaaaaa ccgttcttaa tttgtatgtg agtgcggcca gagcgatccc aattcctgta
     3121 tttccggcag ttggttcgat gatcgtcgtt ttttcattga tttttccttg ggcaaatcct
     3181 tctttgatca aataactgcc caatctatct ttgatacttc ctccaggatt taaatattct
     3241 agttttgcat aaatagcaga acctttaggc atagaatagt gctgattgtc aaagcgataa
     3301 agcggtgtgg aaccaatagc ttctgtaatt gtcgagataa tcatgtgggc ctcctaaaaa
     3361 aagataataa aaaaaggagc tgactattaa atcagctcct tgaaagacat agagaaaaat
     3421 tgagtagata aaaagataag ttacctggta ctcattctat gaagggcaat taatagacat
     3481 gacaaatagt atgctgcttt tatgcaccat acaacagcaa cgattcaatt gtttttggtt
     3541 gctgactgtc atgtgtatcc ctccaagtaa aatttttatt aacgatagca tgaaaaaaaa
     3601 taaaagtcaa aggcttgaaa gaaaaaaata tttcgatagc atcgtttacc cgtgtttatt
     3661 tttctgacaa ataggcatga gaccttaaaa gatagagaat actctagttt tctggccggt
     3721 tgtctgatac aataaagttg attatgataa agaaaagaag gagcaaaaat aatgatctca
     3781 gcaagtgatt taagagccgg tatgactttc gtacaagacg gcaaactgat taaagttctg
     3841 gatgcaagcc accataaacc tggtaaagga aatacagtta tgcgtatgaa actgaaagat
     3901 gtccgttcag gtgcaacata cgacacaacg ttcagaccag aagaaaaatt tgaaaaagca
     3961 catatcgaca caaaacctgt acaatatttg tacacgatgg atgatacagc tttcttcatg
     4021 gatttggaaa catatgagca atatgaaatt cctgtagaaa cagtagaaga agaaatgaaa
     4081 tatatcctag aaaacatgga agtaaaaatc caatttttcg gtagcgaagt catcggcgtt
     4141 caattaccaa caactgtcgt attacgtgtt gtggaaacac aaccttccat caaaggtgca
     4201 acagtcactg gttcaggtaa gccagcgaca atggaaactg gcttagtagt caatgttcct
     4261 gacttcatcg aaacagatga attgttggaa atcaatacag cagaaggttc ttatgtaaaa
     4321 cgtgcgtcaa aataattcgc taacggattg ataagaattt gaaaacagag cttgacctct
     4381 tgaaaggcag atcatcggat ctgcctttca agaggttttt tgtggaatca gcaaattgaa
     4441 aaataaaaaa gtatttgatt ttatttatat tttgaaagat gaatggcaaa agctagagaa
     4501 agaaaaaact gcacttattt atgaagaaat tgaaatgatt cataaaattg aaaatatccg
     4561 aactattccg aactatattg aagcgaaaat attttttcat cactaaatag tgttgaattt
     4621 atgtttttat gtataaatag cttctaatag ttcgggtatt ttgtttttat aaccattttt
     4681 attcttattt ttaaaaaaat atttcgaaat atactagaca attcagaaaa ttttgtttaa
     4741 tattattcca ataaaattca aatctttatc aaatgaaatt ccaataaaaa cggaaaaaaa
     4801 gtgaaaagag gaatgaaaaa atgacagaat tgataattgg cggagtcaca gcaagcacat
     4861 tggcagaaaa gtataagaca cctctgtatg tgtatgatga agaaaagatg agacaaacga
     4921 tgctgtcatt caagcgaggc tttgtatcga aacgttttga tacaaaagtg ctttacgcct
     4981 ctaaagcatt ccaaacgatt gagatgctgc atttagttcg tgaatatgga ttcgggctag
     5041 atgttgtcag tggtggggaa atctataccg ctttgcaagc tgattttcct agagatacta
     5101 tctacttcca tggtaacaac aagacgccag aggaactgat ctatgcactt gaaaatgatt
     5161 tgttgcatat tgttgccgat aatttgatgg aagttgaatt attagcagaa ttaagccaac
     5221 gctatttaaa aaaaatctgt atcatgcttc ggttgaatgt cggagtagaa gcacatactc
     5281 atgaatatat cgtcactgcg catattgatt caaaattcgg catgagctat gaaagtaaag
     5341 agtgccaaca agcccttcag ttgataaaag aaagcagcta tctagagtta gaaggatttc
     5401 atgctcatat cgggtcacaa atttttgatg ttactgcttg gttagctgaa atcgacaaat
     5461 tgacgaatta cttaaccgat tttgaccaac ctctttcctt gaatcttggc ggagggtttg
     5521 ggattcgtta tacacaaaat gatcagccga tcccagtcga agaggcacta aaaaaactga
     5581 ttgaatatac ggaagaagct ttggaaaaac gaaaactgac cattcgtcaa ttactgatcg
     5641 aaccgggtag aagtattgtt ggagaagctg gtacaacact atacacgatt ggtttcatca
     5701 aaaagacacc acataaaaaa tattactttg tagatggcgg gatgacagat aatatccgtc
     5761 cagctctgta tcaggcagaa tacgattgtg atttagcagc aaaactgtcg gaagaaaaaa
     5821 cagagaaagt gacgattgca ggaaaaatgt gtgaatcagg cgatgtggta attaaagaag
     5881 cttatttgcc gaaagcagaa tcgggtgatc ttttagctgt ttattccacg ggagcttatg
     5941 gatactcgat gagcagcaac tacaatcgag cgacacgacc agcagttgtt tttgtcaata
     6001 atggccaatc aagactgatt gttcgcagac aagcattttc tgatctacta aggggggaag
     6061 tttcctatga tccttcataa atataatggc tgcggaaatg attttatctt attagattac
     6121 gcagaagaca cagattatag tcaattagcc gcttctttat gcacaaaaga aaaatatgat
     6181 atagacggat tgatcgccgt aaaaatcgat ccgttggaaa tgatctatta caacaaagat
     6241 ggcagccgcg cgccgatgtg cggaaatggc attcgctgtt ttgctcgata cgtaaatgat
     6301 cagaacatga tgaatgaaca ggaattcaac gtagagacat tagcaggcac aagaaaaatt
     6361 acggtgttgg atccctcacc tttttattgc agcgtagata tgggagtgcc agatgattct
     6421 ccggaaaaaa tcaaagctaa tcaaacttat ccaataaaaa accaggaaat agagatcaat
     6481 ggtgaaaaag taacgatcac tagtttattt ttaggaacga ttcataccgt tgtttttgtt
     6541 gaaaatgcgc gtgaggaagt ctggagaaaa agaggtgaac tgatctgtca tcatcctttg
     6601 ttcaaagaaa aaaccaatgt caactttgta gaagtagtca atgaacaaga attgatcgtc
     6661 cgtacctatg aaagaggagt aggttggaca cttgcttgcg gtacaggttg ctgtgcctct
     6721 tatgtggttg ctaaggaaac tgggaaaatt tcagcagatc aggtagttgt tcacttggaa
     6781 caaggagact tgatgatctc tggtgaggat gcgatacaaa tggctggccc agcagttcat
     6841 gaatggacaa gaatattgga ggaatgaaga tgataaaagg ttcgatcgtg gcgctgatca
     6901 cgccgatgaa tgaagaggga tcagtagatt atgctggttt ggaaaaattg atccaattcc
     6961 atttagatga acaaacagat ggattattag ttttaggtac aacaggagaa tcttctacgc
     7021 tcacacaatc tgaggaggag cagatccttc aactgacagt aaagaaagtg gctggccgtg
     7081 tgccggtgat tgcaggagct ggaacgaata atacgaaaga aacgattgaa aaagcgaagc
     7141 attttgcttc tttgggggca gacgcacttt tggtcatcac cccttactac aacaaaacaa
     7201 gtgatgcagg actagctgct cattttacag caattgcaga agcctcaccg attccgctta
     7261 ttttgtataa tgttccttct agaacaggca tgtcgattcc aatccatgta ttagtaaatt
     7321 tagcagaaca tcctaatatc attggtttga aagaagcctc aggagatatg gcttatgtga
     7381 tggatgccgc tcggttgatt ggagaagaat tcttccttta cagtggaaat gatgatttga
     7441 tcctaccagt aatgagtgta ggaggaagcg gtgtaataag tgtatgggcg aatatccaac
     7501 ctaaaatagt ccatgaatta gtaaaagata cgcaggacgg aagatggcag caagcaaaag
     7561 aaaagcaatt aaatgcactt gaactgatcc atgccttgtt cagtgagacc aatcctattc
     7621 ctgtcaaagc agccatgtca ttattagact taccttccgg acctctacgc ctgcctttag
     7681 tttctctttc aaaagagaaa aagaaacaat tagctcagct tttgttaaaa gaaaggacgg
     7741 ttgaaaaatg aaaatcggac tgattggcag tggacagatg ggaaatagag tcgaagaagt
     7801 tgcacaagaa agaggagata cggtccttct tttacggacg gcacctaaag aagcaactac
     7861 gcttgctttt acggaactgc cagaactgct tattgatttt tctcatccag ataatttaga
     7921 gaaaatcctt tcgtatagct tatcatatca acttccgtta gtcattggca cgacaggtta
     7981 tacacaatca cagttccaag aaataaaaga acatgccaaa catgtacctg tgatgtatag
     8041 tgctaatttt tcgttcggca tcatggtgat gaatcaacta atcaagcaag cagctgcaat
     8101 gttgcaaggc tggcagatcg aactgattga aaaacatcat agtcgtaaaa aagatgcacc
     8161 atctggtact gcaaatatgc tgcttcaaac aatccaagaa gtgcaaaagc tgcagcccgt
     8221 atacaactgg caagggaaaa aaagagaaga aaaccaaatt ggtgtacaca gtattcgtgc
     8281 aggttcttta cctggtgaac atgatgtatt gtttgctgct actgatgaaa tatttacgat
     8341 caaacatgaa tctttttcta atcgaatctt tgctgcaggc gcagtagaag cagcggactg
     8401 gttgaaagat cagtcaccag gttattacaa attagaagat ttattaatgg acaaaggggt
     8461 gtaaaaaacg tggaagctca agaaatcatc cgctacattc aaacggcatc taaaaagacg
     8521 cctgtgaaag tctatctgaa tgtaaccgag ccaatcgaat ttaaagaaag taaagttttt
     8581 ggtgaaggaa gcagcttcgt tgtttttggt gactatgaga cgattgctcc ggttttagaa
     8641 gctgaatcag aaaaaatcat tgaaatcgaa atcgaaactg ctgcaagata cagcgcagtt
     8701 cctttgctag atattaaaaa aattaatgct cgtatcgaac caggtgctat tatcagagat
     8761 caagtgtcaa tcggcaacaa tgccgtgatc atgatgggag cgattatcaa tattggtgca
     8821 gtaatcggtg aaaacacgat gattgatatg ggtgctgtct taggtggacg agcgacagtg
     8881 ggcaaaaact gccatattgg tgcaggagct gtcttagcag gtgtgatcga accagcatcg
     8941 gccaaaccgg ttatcgtaga agacggcgta ctggtaggtg cgaatgcagt gattgttgaa
     9001 ggtgtccata tcggaaaaga tgcagtcgtg gcagcaggag cagtggtttt agaagatgtg
     9061 gcagcagaaa ctgttgtcgg tggaatacct gcacgtgtat taaaaataag tgatgatcag
     9121 acaaaagaaa atacagcatt gattgctgcg ttacgcgaat tatagaggag atggagaaaa
     9181 atgcgcgaaa tagatacctg tattgtgggg gcaaccggat tagtcggcca aacgatgctc
     9241 aaagtgttag aagaatatga ttttccagta ggacgattga aattattagc ttcaaaacgt
     9301 tcagccggta aagtttgtgt attcaagaaa aagaaacaca ctgtacagga attgacagaa
     9361 acttcatttg aaggatatga tttagcgcta ttttctgcag gtgcttctgt atccaaagag
     9421 tttgcgccaa tcgcacgaag tcagggacta tttgtcatag ataattcctc agcatggcgg
     9481 gaagattcgg atattccatt aatcgttcca gaggtcaatc tggaagacta tacattgaat
     9541 cggctgattg caaatccaaa ttgttcaacc attcaagcgg tacttccgct taaagcttta
     9601 caagaagcct ttggtatcca gcgagtcaat tattcgacct atcaagcagt ttctggttcc
     9661 gggcaaaaag gcattgaaga tcttcatcgt acgcaaaatg acgaggaacc agttttgtat
     9721 ccttataata ttagccaaac ggtgattccg gaaatcgatc agcctttgga aaatgggtat
     9781 acaaaagaag aacagaaaat gataaatgag acccgtaaga ttctgcatca gcctgctttg
     9841 ccagtatcag caacctgcgt acgtgtgcca atcgaaaatg gacatggagt ctctgtggca
     9901 gttcaactgc aaaaagagtt tacgacggaa gaaattagag agtgtctcag ccagtttcca
     9961 gggattcaat tagaagatga tctagcagca cataaatatc cgacgagcat tcttgctaga
    10021 ggaacagata tggtttatgt tgggaggatc agaaaagaca cttctttaga gaacggattg
    10081 ttgttctata cgactgccga taacatacgc aaaggagccg ctgcgaatgc cgtacagatt
    10141 gctgcagctt taatgcagaa aatagcggag gtggcaagat gagtaatacg ctgaaagtag
    10201 cgaaattcgg cggaacatcg atgtctcatg aagagcaatt caaaaaagtc cgtacaattg
    10261 ttctttcaga tcccaatcgc caagtgatcg ttgtttctgc tttaggcaaa cgtacgtcag
    10321 atgatgacaa agtaacagac ttactttatt tgatttatgc ttaccttcag catcatgttg
    10381 attggacacc cctttggaaa aggatttgtg atcgtttcat tcaagtaaga gatgttcttg
    10441 ccctgcagtt acctattgaa gatgaactag cacgcataca gaaacaactg gaagacgaaa
    10501 agataactga agattattta gtaagtagag gtgaatatct gactgctcaa ctgatggctg
    10561 agtatcttgg ttaccagttt attgacgcaa aagacctgat tttctttgac tatgatggga
    10621 aaatcgatca ggaaaaaaca gcacaggaaa tcacaaaagc atttaaaaga tatccgaaaa
    10681 tggttgttcc agggttttac ggggcgaacc ctgcaggaaa agaaaaatta ttgggccgtg
    10741 ggggaagcga cattaccggc gcgatactgg cgaagatgtt gcaagcggaa agttatgaga
    10801 attggacgga tgtttcagga atcatgatgg ctgatccgcg aatcatcgat catcctaaaa
    10861 tcatcgagga aatctctttt agagaactga gagagatggc ttatatgggg gctaacgttc
    10921 ttcatgaagc agctgttttc ccagttcaag aggctgatat ccccatccaa ataaaaaata
    10981 ccaatgatcc tttcgctcaa gggacaaaaa tatcgaatca cgaaatagaa aaggaaaatg
    11041 gactgacagg aatagctgga cgcaaggatt ttctttccat cactttattc aaacgtcata
    11101 tgtccgatga aatcgggttt atctggaaag caatgagcat ttttgctaaa catgagatat
    11161 caatcgaaca tattccttca gggatcgaca atatcggagt gatcgtctca gcagaagcga
    11221 ttgccgataa gttatttctc atcacgaaag aattaaaaga aacacttggc gtggaagaaa
    11281 tcgtcgtgat cgaagactta gctttgatct ctgtcgttgg gggtccgcat aaagaattga
    11341 ttggattatc tgggaaagtg ttatcgatat tgaacaaatt agaaattcgc acatcgatct
    11401 tatcacaagg agcacaggag ttgaatctga tcatcggtgt accgaataat caatatgaaa
    11461 cagtagtgaa gggtatctat gaagggatgg tgaatacgga tgctgaaaga atatcgaatg
    11521 gcactccatc aaattcctga aatcgggttt catgaattca agacaaaaaa atatttatat
    11581 gaacaagtaa aaaacagcga ggccgttatc catgaaatcg gcgaaacagg actactcctt
    11641 tattttgata atcagcaaga agaaacgatt gcttttcgaa cagatatcga tgcactgcca
    11701 gtcaaagaag aaacgaatct ctcttttgct tctaagcatg aaggttatat gcatgcatgt
    11761 ggtcatgacg gacatatggc gatgctttta gggttgactg actttataac taaacacatt
    11821 gaggatctta tttataatgt agtactgatc tatcagccat cagaagaaat ttccggtggc
    11881 gcacaaagcg ttattcagtc aggattgttg gataaatatg aagtacaagc aatatttggc
    11941 tttcatctat ggcctggact agctaaggga gaagttttct ctcgaccagg acctttgatg
    12001 gcacaaagca gtgaaacaga cattatcatt aaaggccagt ctgcacatat tgcttctagt
    12061 gaaaaaggaa tcgatagtct ggaggcagct gtacgatttt tgaagaatgt ttatgatttt
    12121 gaccggcaat taccggcaga gtggaatcat ctaatgaaat ttggccaaat ccaaggtgga
    12181 acgatccgaa atgttttagc tggagaagtt attgtatccg gaagtatccg ttcttacagt
    12241 tcagatatgc aaaattattt gaagagggga ttagaccatt tagcagaaca gtttcactct
    12301 acagctacta tttcttttcg ctataatgac ggctatccag ctgtaatcaa ccatagaggc
    12361 ttatatgcag cactgttaga ttcaaacaaa ctacatgaat tagatgctcc agttcttcaa
    12421 gccgaagatt ttggcatata tacgcaacaa tatccatgtg tatttttctt tttaggtgtc
    12481 ggtgataccc ctgctttaca tgatccgacg ttcgattttg atatggatgt tttagaaaat
    12541 ggtgtagaat ggtacaaaac gattcttttt acagaaaatc ttcttgtaaa aatcgatagc
    12601 agttcaaaag aggagcgagt aaataatgat gaatttatcc aatcgattta gtcctaggct
    12661 ttctagaata gaagtatcaa aaatacgaca attcgatcag caaatatcgt cgataccaga
    12721 tgtgatcaaa ctcaccctgg gagaaccgga ttttccaact cctgaacatg tcaagcaagc
    12781 aggaatagca gcgattgaag aagacttttc tcactatacc ggaatgagag gattagagga
    12841 actgcgggaa gcggcttgta tatttcagca acagcgttat ggattaactt atgatccgca
    12901 aacggaagtt ttgacgacag ttggtgcaac ggaagcgatt gcctctgcgt tgttatctgt
    12961 tttggaagaa ggagacaagg ttcttattcc agcgcctgct tattcaggat atcagccatt
    13021 agtggaacta gctggtgctg aattgatccc gatagatact tctgacacag ggtttgtctg
    13081 tcagccggaa caatttgaac gagcatttga acaatatgga tcagctgtca aagcagtcat
    13141 tttaaactat cctaacaatc ctactgggac gacattgtct gcaaaacaat tgaaagcaat
    13201 cgctgaggtt ttaaaaaaat atccggtatt tgttataagt gatgaagtat atgctgaatt
    13261 gacttatagt ggaacgcata tgtccatagc tacttatctt ccagaacaaa caatcgtgat
    13321 aagcgggtta tccaagtcac atgcgatgac tggttggcga gtgggcttta tttttgctca
    13381 aaaagccctt attgacgaac taattaaagt ccatcaatat ctggtgactg ccgctacaac
    13441 gatgtcgcaa aaagctgcgg ctgaagcgct aataaatggg gtgaaagata gcgagaatat
    13501 gaaagagcaa tatcaaacac gaagagacta tctggtccag caattggcgc ctttaggatt
    13561 tgaagtgacc cagccaaacg gtgcatttta cttgttttgc aaactcccag ctgccattca
    13621 aactgactct tggcagtttt gtgtagaatt agcggaacag gcaaaagttg cgtgtatccc
    13681 aggaattgca tttggcccag aaggagaagg ttatatccgg atttcttatg ctagcagcat
    13741 ggaaaaatta catgaggcct gcaaacggat caaagacttt ttaacagaac agtgattcat
    13801 agattttatc aaaaaaacaa tcgttaattt tgcggtatat tctgcaagga gacgattgtt
    13861 ttttaaattt agttcaaatg aacgcgtttc ttttgattca taactaatgt aatgttaagt
    13921 ttaaattgtt tagaatgatt ttaaaagaat attcaaggag gtttcatcaa tggaaaaaat
    13981 caaagttgtc attgtaggtg catctcatgg agggcatcaa tcgatcttgg aactattgac
    14041 acgatacggt gaaaatgtag atatcacgct atttgaagca ggagactata tttccttcat
    14101 gtcatgtggt atggagctgt atttagaaga caaggtaaca gatgtaaatg atgtacgtaa
    14161 tttcagacca gaaaactttc cgcaatccaa tgttcatatt ttaaataatc acgaagtaaa
    14221 aacgatcgat gccgataaaa aaacagtaac ggttactagc gcatcagata acgaaacaaa
    14281 agaatatcct tatgacaaat tgattttgag ttctggtgtc aaaccaaaca atctgccagt
    14341 accaggagca gatctagaaa atgtctactt gatgcgtgga tacaattggg caaccaaaat
    14401 caaagaaaaa ttaaatgatc cggctattaa aaaaatcgct gtcgtaggtg cgggttacat
    14461 tgggatcgaa gcagcagaag caagccgaaa agctgggaaa gaagtcgtat tgcttgatgt
    14521 gatcgataga cctttaggca cttatttaga tgctgagatg acagatattt tagaacaaca
    14581 cctaaatgaa aacggtattg aagttttgac aaatgcaaaa atcgaagctt ttactggtaa
    14641 cggaaaagtt gaggcaatca aaacaagtga aaaagaaatc ccagcagatc tagtcattca
    14701 agctgccggt gtgaaggcaa atacagaatg gttaaaaggg atcgtcgact tagatgaacg
    14761 tggctggatc caaacaaatg aatacttgca aacgaatctt ccagatgtgt atgcagtagg
    14821 agatgctaca cttgcttatt ctatcccagc acgcaaaaaa atgccgatcg ctttagcgac
    14881 tgttgcacgt agagaagcac gttatgtagt aaaacatctt tttgaagaag taccaaacaa
    14941 accgttcggc ggagtcgtag gttcctctgc attaagtgtc tttgactatc attttgcagc
    15001 aagcggattg aatagcttta cagcagcaaa agcaggagta gatgtacgag tgtcttatta
    15061 tgaagatacg ttacgaccaa aatatgtacc agaagaacaa ggaaatccaa aagtgtccat
    15121 ccaattattc tcggatccaa tgacccatca attgctagga ggagcagtat tatctaccta
    15181 tgatgttaca ggtcaaggaa atgtattggc actagcgatc caaaacaaac tgactttaga
    15241 agaactagca gaagctgact tcttcttcca gccaggcttt gaccgacaat ggagcatgtt
    15301 gaatctagcc gctcaacaag cgttaggaga agaaccattt gtagaataag aaaacgaaat
    15361 atttgaa
//
LOCUS       DBNICI010000059        14973 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.059, whole genome shotgun sequence.
ACCESSION   DBNICI010000059 DBNICI010000000
VERSION     DBNICI010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.059"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..577)
                     /locus_tag="AC1ZCC_001477"
     CDS             complement(<1..577)
                     /locus_tag="AC1ZCC_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIQ1629738.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
     gene            719..1651
                     /gene="pfkB"
                     /locus_tag="AC1ZCC_001478"
     CDS             719..1651
                     /gene="pfkB"
                     /locus_tag="AC1ZCC_001478"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HIQ1629739.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            1633..3096
                     /locus_tag="AC1ZCC_001479"
     CDS             1633..3096
                     /locus_tag="AC1ZCC_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HIQ1629740.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            3093..3551
                     /locus_tag="AC1ZCC_001480"
     CDS             3093..3551
                     /locus_tag="AC1ZCC_001480"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HIQ1629741.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            3548..4522
                     /gene="lacD"
                     /locus_tag="AC1ZCC_001481"
     CDS             3548..4522
                     /gene="lacD"
                     /locus_tag="AC1ZCC_001481"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HIQ1629742.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            4963..5691
                     /locus_tag="AC1ZCC_001482"
     CDS             4963..5691
                     /locus_tag="AC1ZCC_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIQ1629743.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            5709..6905
                     /locus_tag="AC1ZCC_001483"
     CDS             5709..6905
                     /locus_tag="AC1ZCC_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIQ1629744.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            6898..7893
                     /gene="lacD"
                     /locus_tag="AC1ZCC_001484"
     CDS             6898..7893
                     /gene="lacD"
                     /locus_tag="AC1ZCC_001484"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HIQ1629745.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            7890..8831
                     /locus_tag="AC1ZCC_001485"
     CDS             7890..8831
                     /locus_tag="AC1ZCC_001485"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HIQ1629746.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            9007..9486
                     /locus_tag="AC1ZCC_001486"
                     /pseudo
     CDS             9007..9486
                     /locus_tag="AC1ZCC_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            9499..10389
                     /locus_tag="AC1ZCC_001487"
     CDS             9499..10389
                     /locus_tag="AC1ZCC_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HIQ1629747.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            10376..11185
                     /locus_tag="AC1ZCC_001488"
     CDS             10376..11185
                     /locus_tag="AC1ZCC_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HIQ1629748.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            11199..11600
                     /locus_tag="AC1ZCC_001489"
     CDS             11199..11600
                     /locus_tag="AC1ZCC_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIQ1629749.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(11646..12596)
                     /gene="yidC"
                     /locus_tag="AC1ZCC_001490"
     CDS             complement(11646..12596)
                     /gene="yidC"
                     /locus_tag="AC1ZCC_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HIQ1629750.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(13156..13431)
                     /locus_tag="AC1ZCC_001491"
     CDS             complement(13156..13431)
                     /locus_tag="AC1ZCC_001491"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HIQ1629751.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            13539..14303
                     /locus_tag="AC1ZCC_001492"
     CDS             13539..14303
                     /locus_tag="AC1ZCC_001492"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIQ1629752.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            14426..14929
                     /locus_tag="AC1ZCC_001493"
     CDS             14426..14929
                     /locus_tag="AC1ZCC_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIQ1629753.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
BASE COUNT         4801 a         2464 c         3261 g         4447 t
ORIGIN      
        1 atttaaaaaa ttgaaaaata aagacataaa tcttattttt gatgttttta tttgttttct
       61 caccgttcaa agaaacaaaa ataacgctgt ctttcttagt tagtttccga ctgatgcttt
      121 tgataatttc tggatcagta tgcatctgac aattttttcc cgttaattga agttttacca
      181 gcatttcttg gccaatcatt tctgaaaatc ctcgggcaaa gacaaaaacc cggtcagcaa
      241 atttgattct ttgtattgca tcttcaatgt tcccgatttc caacatattg attgtacgta
      301 ccacttcttg ttcatttttt agaatggctc ggcggattcc tttgtctact ttatctaaaa
      361 atgaaaaatt gatgttcttg ttttctcctt cttttagatg atgcttaaac gaagtaaacc
      421 cttcatagcc tttctttttc attgtccgca caatggtggc tgttgagaca tttgcttgtt
      481 cacttagttt cacgatggaa tagttaggga tctctaggat gaggtgttcg ataaagttcc
      541 agaggtactg ctctgtttca cttaatttgt gattcatatt ctttccttct ttcaccaaaa
      601 gttagttaaa aaaagcacga cgagtttata aatgtaaagg ttttctttgt gttgggacga
      661 ttaagaaaac attttctatt tcgcgtattt cataatagaa tcaaggaggt cgttggttat
      721 gtctatctac acatgcacga tgaatttggc aattgatcta tttattgaaa cagatgagat
      781 gcatcctttt atggtaaacc gcacaaaaga ggatgacatt caggcaaacg gaaaaggggt
      841 gaatgtgtca ctagtattaa agatgctaga tttcccaagt acggcattag gtttttctgc
      901 tgggttcaca ggaaagtata tagaagacta tttacagcag aaacaaatcc aaacagagtt
      961 tattgaagtg cctggtatga cgcgtatcaa tgtatttaca caagtcaatc aaacgggaga
     1021 agaatacaaa ttagtgaatc aaggacctga aataccgcag acagctgttc ataatttttt
     1081 aaatcaaata agagcattac aagcagggga ttatctttgt gtttctggga gtttaccaag
     1141 agggctgtct ccaaaggttt taatagaaat cagtcggatt tgtttgatta ttgatagtag
     1201 tgatcaagaa gtcatggatt gtttacccta tcgaccgttt ttgctgaaac caaatgagga
     1261 agaactttct tcttggttcg gacgaaaaat ggttacagat gaagattact ttgtttatgg
     1321 acaacgacta gtagagtcag gcgcagaaaa tgttttgtta tccctcggtg agaaaggagc
     1381 gattcttttt acgaaagaca gggtgattcg aggaaactcg ccaaaaggaa aagttgtcaa
     1441 tacagcatgt gcaggtgatg ccatgttagg cactttttta gcaggataca tgaatcgacg
     1501 atcgcttgat gaaacgttac gtaaaagcat agcagcaggc agctctactg cttttcgtaa
     1561 aggactgact gattttttgg atgtggagga attaagtaaa caaatcaaaa tcaaggaaga
     1621 gaggttcgaa agatgggaaa atatcaacta attgcagcaa ccggttgtcc gacaggaatt
     1681 gcgcacacct acatggcgca agaggcatta gagcaagcag ctaggaaaaa aggcatcacg
     1741 atcaaagtgg agacccatgg acaaattggg atcgagaatg aattaacggc tgaagaaatc
     1801 aaacaggcag atgctgtcat tattgccgca gacaaagatg tccattcaga aagatttgtg
     1861 ggaaaacgag tcattgatgt ttctgtcagt acaggaatca aggatgcaca acggctgatt
     1921 gacgaagcat tggcaggaaa aggtactgtc ttagcagata atgaagctgc tgatacttta
     1981 gaaccagaag cgataagtgg tgctaatttt ggtcgtagca tctataaaaa tttgatgaac
     2041 ggtgtctctc atatgttgcc tttcgtggta gctggcggag tattgattgc tatttcattt
     2101 gccgtttggg gaatctattc ttctgatcca gatcatgcac aatacaatgc tacagcggca
     2161 atggtaaaag cagttgacga tgcagcaatg ggaatgatgg ttccagtttt agcagcttat
     2221 attgcagaag ggatcgcgaa acgtccaggg ttagtcgttg gatttgttgg tggaatcgta
     2281 gccatgaatg gcggtactgg atttttagga ggtattttag ctgggttctt agcaggttat
     2341 tttatcttat ttttgcaaaa gctactgata attttaccta aacaactaga tggcttgaag
     2401 gcaatcgtct tatatccagt tattggtgta gcatttattg gcatcgtgat gtctttgctg
     2461 gcaggtccaa tgaaggctat aaatgaagga atgatgagct ttttagctgg atttgaatat
     2521 tcgaatccac ttgttcttgg cgtgattgtt ggctgtatgt gtgcgtttga tatgggagga
     2581 ccggttaata aggcggcata tgtaacagga acagctcttt tgacacaagg aaacacgact
     2641 tttatggctg gtgcgtcagc agcttgcatt gcaccaccgt tgattactgg ttttgctact
     2701 ttattgtttg gtaaatattt tgatactaat gatcgtaatg caggtttggt taattttatt
     2761 ttaggatcaa ctcatattac ggaaggagct attccgtttg ctgcgaaaga cccgatccga
     2821 gtattaccaa cgatgatgtt aggctcatca attgctgctg tcttgaccta tctgtttggt
     2881 gtccaagtac cagccccaca tggcggtttt ttagtgttac ctgtagtgac gggaaaaatc
     2941 caatgggtta cagcgattct tattggttcg atcgttggtg gagtgatact aggtttgatc
     3001 caaaagaaaa gagtaactga taaagacaag aagagggata ataaagcaac aaatcgtaca
     3061 gttatcgaag aaggaagagc aggaggttta tcatgacaat cattcatgaa gagaacattt
     3121 ttttacaaca gccattagat aatcaagaag aagcatttaa gtttttagca aagcaaacat
     3181 tctgtttagg aatcgctcat gacgaaaaag aagtatatca aaaacttctg gaaagagaaa
     3241 aagaaggtac tacaggaata atgtcaggtt ttgctatccc tcatgcgaag tctcaaacaa
     3301 tcaatgaacc tagtttgatt atcgtcacac tgaaaaaagg aattgaatgg aacagtttag
     3361 atagacagtc agttgatttt atttttgcgt tatttatccc agattcagaa gcaggaacga
     3421 ctcatttgaa attattgtct ctcgttgcac gatcattgat gcgaaaagag gttacagaac
     3481 agttgagagc tgcaaaatca aaaatagaaa tagctacaat attaactaaa gaaataggtg
     3541 aagatatatg aagaaaatga gtatagaaaa acaggaacgt ttgaagcgat tgacggatga
     3601 agctggaatt attagcgcgc tagctatcga ccaacgtggt gctttgaaga aaatgatcaa
     3661 ccaatataaa gaagctacat cagaagatat catccaattt aaacagatgg tttctcagta
     3721 cctgactccg tatgcttccg ctattttact cgatccagaa tatggtctgc ctgcagcagc
     3781 gcttcgagag aaagaatgtg gtttgttgct agcttatgaa aaaaccggct atgatgcgaa
     3841 aatgccaggc cgactaccag atttactgaa tagttggtca gtgaaaaggt taaaagaagt
     3901 gggtgcggat gcttgtaaat ttttattgta ctatgacgtt gatgaaccag tagagattaa
     3961 tgtgcaaaaa caaatagtta tagaacggat tggttcagaa tgcgaagcag aaggcatacc
     4021 tttcttttta gagctccttt cttacgatgc aactattggc gatccgtctt ctcttgacta
     4081 tgcaaaagta aaaccgcata aagtgattga agccatgcga gaattttcaa aagaacgtta
     4141 tcaagtggat gtattgaaag tagaagtacc agtgaatatg agatttgtgg aaggatatgg
     4201 gcaagatact tgttatagtc aagcagaggc atccgcgtat ttctatgaac aatcgaaagt
     4261 gactgaacaa ccgtttattt ttttaagtgc gggagtcagc gctgcactat ttcaagatac
     4321 attgcgtttt gcaaaacaat caggttcgac attcaatggt gtcttatgtg ggcgagcaac
     4381 atgggcgaag ggagtagcac cttttgttac cgaaggcgaa gaaaaagcga gtagttggat
     4441 gttagaagaa ggaaaacaaa atattaaaga attaagtaca ctattagaag aaacagcaac
     4501 ttctatttat gttaacaagt aagagaaagg attgtctgag tgacagattc aaaaaataat
     4561 tagttattta aaaaaatgtt tgtcaaattt tatagaaaaa aagtataaaa ctgcaaaagt
     4621 tatctaaaac taataataac atttgtcatt aataagctat ttttagttgt tgtaaatgat
     4681 attggaagta ataaaacgat agttgtagga atatcatagg gattaatgtg gatcacgctg
     4741 ctcgataagt tagaccattt tgacggatcg cttatgtggt tcgtcttttt ttctgttctt
     4801 ttattggtaa ctggtatttt tgaggtatct ttgtctattc aagatataaa gctttaaaga
     4861 gctgttataa tagctttttt ggtctaaaaa acctttgaca aatgattaat aattcactat
     4921 tcttgtgttg tggttataac cagtaaagaa gaggtgattt ttatggagag ctcaaataaa
     4981 ggaaaatcat tgtattatca actagttgat ttattgaagg atcgtatcga aacacagatg
     5041 actccgcatg ataagctgtt atctgaaaga gaactttgtc aacactatgg ggtgagtcgt
     5101 accaccgtac gtctggcttt acaggaattg gaaaatcaag gttatattta ccgaagacat
     5161 ggaaaaggta cctatgtctc tgatttgtct gataagtcat cgaacttagc tggagcatat
     5221 agctttacag aagaaatgcg cgaaataggg cgcgtcccta agacggtcat tttagaattt
     5281 agacaaatgg aagctaataa gagaattgct aagaatttga acatccattt aggtgaaact
     5341 gtatttaaac ttaaaagatt gcgtttggca gatgaagagc cattgatggt tgagcgttct
     5401 tatctaccgg cgaaactatt tatgggactt gatttgccgt tacttgaaag taaaccgttg
     5461 tatgatgtgt tttcacagga ttatgaacaa caagttcaat tagctgatga agagttttat
     5521 gcaagtatcg tgcaaaaaaa agatgctgtt gtgctacgaa tcgctgatgg cgctcccgtc
     5581 ttacatctga cgaggataac ctataacgtg aaaaatgaga ttatcgaata tacagatagt
     5641 gtagcaagag cggaccagtt tcattataaa attcggcata tccgaaaatg attgagaggg
     5701 agaacaacat gtttgagcta tcgaagaaca aattaaaaca aataggtgca gcaatcacga
     5761 cacaagaaat aaaacaacaa ccagatctct ggaaagaaac aatcgataga ttgatgcaaa
     5821 gccaagaaaa tttagctcta ttttttgatc aaataaaaaa agaagcaaac ggaagaagaa
     5881 tacgtgttat ttttacgggt gcaggaacgt ctcaatatat tggggatacg ctcttaccct
     5941 atctaaatga acatggagac aatcagtttt ttatcttcca cagttttggc acgacagata
     6001 ttgtagcttc accaaaagaa tttttgtttc caaatgaacc gactttatta gtttcatttg
     6061 ctcgcagtgg caacagtcca gaaagtgtag cggctgtgca aattgcaaat gaacaagttg
     6121 aaacattgtt tcatttgacg attacttgtg ctaaagaggg gatgttggca aaaaacgcac
     6181 aggatgataa acagaattat ctgttattga tgccagagag agcaaacgat gcaggttttg
     6241 ctatgacagg aagttttacg tgtatgttat tgagtgcttt atggatcttt gatcaaacag
     6301 caatggagaa aaaagaaaga tatcttgaac aattagttaa aatcggtcaa gatcttcttt
     6361 caagagatca agaaattcaa aagttattac ctgatttttt tgaacggatc gcttatctgg
     6421 gttcaggaag tttggcaggt gcgacaagag aagcacaatt aaaaatttta gaattaacag
     6481 caggtaaagt tgctacaatt tttgattctt cgatggggtt tcgtcatgga ccaaagtctt
     6541 ttttaaatag cgagacactc gttgtaggtt ttgtgagcaa tcaaccatat acgcgaaatt
     6601 atgacttaga tattttagag gaaataaaag aagatcgggt cactgccaat gtcattgcga
     6661 ttgcgcaacc gggtactatt gatttctcag gtatgactgt gctattggaa gactcggtga
     6721 gtaaattgcc cgatgcctat cttgcattag cgatgattat gactgcacaa atcattgcac
     6781 tctatacttc aatcaaagta gctaataaac cagatacacc atctccaaca ggcacggtca
     6841 atcgagtagt caagggcgtc attattcata agtatgaaac cgaaggagaa atgacagatg
     6901 aatgaagaaa aaaatagggc aatggagaga ctatcaacaa aagatgggat tattagtgca
     6961 ttagcgatcg atcagcgtgg tgctctaaaa aaaatgatca aatcgttggg aaaagagcca
     7021 agtgaagacc aaatcagtga attcaaagaa ctagtatcag ctgagctgac accctatgct
     7081 tctgctattt tattggatcc ggaatatgga ttgccagcag caaaagtcag agcaaaagag
     7141 acggggttat tgttagctta tgagaaaacc ggctatgacg caactacgcc tggtcgtcta
     7201 cctgatttat tagcggattg gtcttctctc agattgaaag aacagcaagc ggatgcagta
     7261 aaattcttac tttactacga tgttgatgaa gcacccgaaa tcaatcattt gaagcatgtg
     7321 ttcatcgaac gtttaggtag tgagtgcgaa ggagaaaatc gtcccttcta tttggagtta
     7381 gtttcttatg acgcacaaat aagcgatagt tcttctccag agtatgcgaa agtcaagccc
     7441 cacaaagtta ttgagatgat gaaagaattt tctgatccaa aatataaagt tgatgtgttg
     7501 aaagtagaag taccagtcaa tatgaattat gtgaatggct attccacagg tgaatttatt
     7561 cacagcaaag aagaggctgc tgccttcttt aaggagcaaa gtgaagcgac tccattacca
     7621 ttcatttttt tgagtggggg tgtttcaaca gaattatttc aacaaacact gatttttgct
     7681 catgaatcgg gttccacctt caatggtgta ttgtgtggac gtgcgacatg gaaagatggg
     7741 gtcaaagcat acgtcgaaaa tggaaaagac ggagctattc attggttaag aacaaatggg
     7801 agagaaaaca tcgagacttt aaataaagta ataaatgaaa ccgctacccc ttggttcaat
     7861 aaaacaaaaa gcaaaaaggt gaagatgaaa tgattacgac agttaccttg aatccttcca
     7921 tcgatatcgc gtatacaatt gaagaattaa acctcgacaa agtcaatcga gttgaaacag
     7981 tcaataaaac agcgggagga aaaggagtga atgtggcgag ggttctaaat ttattaggag
     8041 aagaggtgat agctactggt ttaataggtg gccagcacgg tgaatatcta aagaaccagc
     8101 tagatcagtt agggatcatg catcagtttg ttccaattaa aggagagaca agggattcta
     8161 ttgctatcct gcatagttcg agacaaacag agcttttgga acgaggcccc ttcataacaa
     8221 agaaagaaca acagacattt accgatcatt tcgagcagct cctcgaaata tcagatatta
     8281 tgacgatctc aggtagttta ccccaaggat gccctcttga ttactataag caattggttg
     8341 ctttagctga taaaaaaggg aaagacatat tactagatac ttctggtaaa tttttgcttg
     8401 aagtattgga aggtaaggag aagccactac ttatcaagcc caatctggaa gaattaggag
     8461 aactgattgg ccaacgtatc tgttcttcga ttcaattaga aaaaattcag tccttattag
     8521 ctcatccaat atttgaagga gtgccatggg ttgttgtttc tttaggagag agaggtgctg
     8581 tggcaaaagt tgctcagtgt ttttacgaag tcattattcc taaaatatca gttgttaatc
     8641 ctgttggttc aggtgacgcg acccttgcag gatcggccca tgcgttatca aaaaggcaat
     8701 caatcgaaga gattctcgca ttttctatgg cttgtggtat ggcaaacgcg caacaagaga
     8761 aaactggctg gatcgaccct atgaaagtta cgatgttgat gaaacacatt caagttaaga
     8821 aaatcaaatg aacttaaata taaaagaaag cgttttcttt agttgggaag gaatttgttc
     8881 ttagcttaaa aatgaaatat aagaaagaaa aaatgaaaac gttatctttg aaacagaagg
     8941 gatatttgat gatgtcacag ttttaattga acaataaata gaaaaaatag ataaggagaa
     9001 tgcatgatgg caattattgg tgtaagaatc gatggacgtt tgatacatgg ataagtagca
     9061 aatctttgga caacgaaact gaatattagt cgaatcatgg tcgtggatga tgaggtagcc
     9121 acaaacacaa ttgaaaaaag tggattgaag ttagcgactc cagcaggggt aaaattaagc
     9181 attttgccaa tcgaaaaagc agcaacaaat attttagctg gcaaatatga ctctcaacga
     9241 gtattgatcg tagcaaaaaa gccagatcga atcttatctt tagtagaaaa aggagttcct
     9301 atcaaagaag taaatgtggg gaatatgtca caaacgaatg aaacacggtc cattacaaaa
     9361 tcaatcaatg tggtagatgc agatatcgaa gtatttaaaa aattagatca gcttggtata
     9421 catttaattg ctcaaatggt accaaacgat aaagcagaag actttatgaa tcttttaatc
     9481 aaataggagg aatgatccat ggatattcaa tggtggcaga tcttattatt aacattatat
     9541 gcaggttatc aaattttaga tgaattacag atctattctt caatgagtgc gccagtgttt
     9601 gcagggttat atgcaggttt agtaatgggt gatttaaacg caggtttgat tattggtggc
     9661 agtatgcagt tgacagtttt aggagtgggg acgtttggag gcgcatcaaa aatagatgcc
     9721 aactctggga cgattttagc aactgccttc tcgatttctc ttggaatgaa tccagaacaa
     9781 gcgattgctg ctattgcagt gcctgtagca agcttaatga tccaattgga tattttagct
     9841 agattcgcta acacatactt tgcccatcgt attgaccgtc acgtagaaga catgaattat
     9901 caagcaatcg aacgcaattt cttaatggga gctttacctt ggtcgctctc tcgtatgatt
     9961 cctgtatttt tagctttagc atttggtggt ggattagttg aaaatgttgt ttctgtttta
    10021 aacgggaatc taaaatggct aggcgatgga ctttcagtag caggagctgt tttaccagct
    10081 gttgggtttg ccattcttct gcgctattta ccagtaaaaa aacatctatc ttatcttatt
    10141 ttaggattta cgatcacggc attattagca acgttattct ctaatgttca attattagga
    10201 acaagtgtgg caagcgttgt taaagatctt ccggcaactt tcaattcgct gccgatgctt
    10261 gctgtagcat tgattggtct agcactagca atgatcagtt ataagaatga tcaaaacaaa
    10321 gaccatcaac cattagctag tgagccatca cattctgttg agggggaaat cgaagatgac
    10381 gaaatctaat tacaaattaa caaaagaaga ttttaagcaa atcaatcgaa gaagtttgtt
    10441 cacttttcaa ttaggttgga actatgagag aatgcagggt tctggctatt tatatactat
    10501 cttgccacaa ctaagaaaaa tatatggaga caatacacca gaattaaaag agatgatgaa
    10561 aacacatacg caatttttta atacttctaa tttttttaat acaatcatta ctgggattga
    10621 tcttgcaatg gaagaaaaag agggaattgc ttcaaaggaa accgtatcgg ggctgaaagc
    10681 tggattgatg ggttcctttg cagcgattgg agattccgta tttgctgctt tgattccaac
    10741 gatttttggg gctttggcgg cgaatatggc aactgatgga aatccgatag gtgtgtttat
    10801 atggattttt gctcagatcg ttgtcatggt ttttcgttgg aagcaacttg aatttgcata
    10861 caaagaagga atttccttag ttactactat gcaatctcgc ttaactgcat taacggacgc
    10921 agccacttta ttaggggtat tcatggtagg ggcattagtt gcgacaatga tcaatgttaa
    10981 attttcatgg gcaccaacga ttggcgatgt gacattgaat atgcaaaact atatcgatat
    11041 gatcttacct aaaattttac ctgctgggat cgtcggtggt gtttattggt tattaggaaa
    11101 gaaaaacatg actcctactc gagcgatttt tatcgtatta attgtgtgtg ttgctttatc
    11161 tgcattgggt gtgatttcga aataagggag agcgaaaaat gagtagacaa ttggtattga
    11221 ttagtcacgg aaaattttgt gaggaattga aaaaaagtac agaaatgatc atggggcctc
    11281 aagaggatat ctacacgtta cctttattac cagaagatgg cactgaaaca tacaaacaaa
    11341 agtttctcac tgtcacagat tcgttaaatg attttgtggt actatgcgat cttttaggtg
    11401 gtacacctgc aaatatcatt tctaaactaa taatggaagg acaacagatt actctttatg
    11461 ctgggatgaa tcttccgatg gtcattgaat ttatcaactc acaaatgatt ggttcagagc
    11521 ccgagtttgt tcgtgcagga tgtgaaagca ttgtctctgt caacgaatta attaatggaa
    11581 tagcagaaga ggaagagtaa caaaaaagat gagggcttag ctctcatctt ttttgtccta
    11641 tgtttttatt tccgcttctg ctgctttcct gcgttgcggc ctttgttgtt tgtactgttt
    11701 tttggttgtt tgatttgttt tgttgcttgt ttgttgttag ctggctttgc tgattctgtc
    11761 acgtcttttc ttggagtgac gacttgtttt ggcgggtttt gtttcaactc ttctgccact
    11821 tgtgctttca aacgtggttt catcataacg tttgtgatga atgtttgcag acagctgaac
    11881 acaccaccga ctacccagta aagtgtaaca ccggctggcg cactgatcga catgaagacg
    11941 atcatagcag gagagacgat tagcatcgta cgcatcgtct ttttctgttc ttcaggaata
    12001 ccgatcgttg agatgtatcc ttgaagcaga taggcgaccc ctgcaatgac aacaagtacc
    12061 atacttggtg agcctaaatc gatgccgtag aaagatgatt cccgaatacc ttctgtataa
    12121 cgtgccgtaa agtacaaggc agagaagatc ggcatttgga tcaatagagg cagacagccg
    12181 attccgccgg tcatactcaa tccgttttcg cggtatactt gctgcatttc tgcttgtgct
    12241 gccatttgtt cttcacgtgt tgttgcagct ttcaattttt gttgggctgc ttcaacttgt
    12301 ggttttaacg cttgcatttt ttcagattgg atcattgttt ttttcgattg actaatacct
    12361 agtggcaaga tgatgatacg gacgatcaca gtcattgcaa taactgccca gccataagac
    12421 caattgaaat tctcaactag atatgtaatc gcttggccca taggatggac gagtactcga
    12481 taaacgattc ccgaagtgtc agggtttccg tttgaatcca tccgcacaca gccagataaa
    12541 aagagcagga tactgacaag tccggatccc aataaccagt ttttccattt attcatagaa
    12601 tacttcctct tctataattt ttacacaaac ttaactatac taaaaaagcg catggaattc
    12661 aatcaacttc atagaaattt catataaata ttcgttcttt tacgtagcga aaaattttga
    12721 aaaaaagaca aaaaatcaag aaagaaatac ttggaaagaa aagagtgatt ccaagtgttt
    12781 ctttcttatt taaaaaacta aaaacttttt tgttctgtct ggaaaatatg ggttaagcaa
    12841 atttattgca agttatagat cagctttttt actacctatc tttaatactt gaggcaaagc
    12901 gtctcaaaaa caaccttatc gacggtgttc aaaagtcagt ttctcggtat taagtcaaat
    12961 tttatgcaaa aatgtggaaa gccattttca caaaattctc cttactactc aaaactaagc
    13021 gactttctca caaccttatt gacggtgttc aaaagcttgc atctacggca ttaagtccag
    13081 cctgttgaaa aatatggaga gctatttttc acaacctgtc cttaatgctt gatgcagacc
    13141 agctttttca caaccttatc ccaccacatc aaatttcttt cgttcctcaa tcatcgggtc
    13201 ttcttggaca tccacatact gcactcgtcc acttgggctc ggagatttct tgacttcttc
    13261 aataaatttc tggatgatgt cgtcattgcc gattgcttcg atcgtgactg agccgtcatc
    13321 ttcgtttttt accgttccag tcacgcctag ttgatcagcg accatcttgg tcatgtagcg
    13381 aaatcctacg ccttggacgc gtccttggac gttcatttta actttgcgca tgttcgtcac
    13441 ctttctttcc tattttataa attaatgata ccgatttttt taggatgaaa caagctcaag
    13501 cacctattcc atgatataat catggggagg tgaggaatgt gaaagagatc caatcagcaa
    13561 aaaatccaag aatcaaagaa ttaaaaaaac tacataaaaa gaaatatcgg gaagaaaagg
    13621 aagaatatct tttggaaggg ttccatctga ttgaagaagc tgcgtcgtct gatgcagaaa
    13681 taaaagaact ttttgtgaca cagcgaggca tgaaagaatg gggagactgg ctggccgcaa
    13741 acaaagagat ttctactttt ctagtttcgg atgaggtcat ggatgctgta tcggatctac
    13801 ctacaccaca aggaatctta gcagtgatgg cgttaacggc tgaaggcacg ccagattttt
    13861 caggaggatg gctgttatta gataatgtac aagatcctgg aaatgtaggg acgatgatcc
    13921 gtacggcaga tgcatttgat atggctggtg ttttattagg caaaggaacg gcagatattt
    13981 atagtacaaa ggtgctgcgc agcatgcagg gaagcaatta tcatttgcct gtgttgcaaa
    14041 aagatttgac agaagtgatt cctttaatga aagacaaagg gtttacagtg tatggcacag
    14101 aattaaatga agaagcacac gccttagaca aagtagaaaa atcagataac ttcgcaataa
    14161 tcatggggaa cgaaggtcag ggggtaagtc aggaaatctt gtctttgact gatcagaacg
    14221 tatatatccc gatgaaagga aatgcggaat cgttgaatgt tggggttgcg gcagggattg
    14281 ttctttatca tttttcttta tgattaagga actattaagc tttggctatt ttatccaagg
    14341 cgactagaat aatccgaaca ctggtggtat acttagatca atttcagaag aaaatgaaat
    14401 tcaaaaaggt ggcagatcga taattatgac agaaaaatgg cgtgaagacg aagaatatat
    14461 gtcttatgta gaagatcttt tagaaacaga agcagtgaaa aaattagcga attacacaca
    14521 gcatgttcat tccactcgtt tggagcattc catcagtgtt tcttactata gttatctgct
    14581 cgcaaaaaaa tggggtggca atgctaaagc aacagcgcgt gctggacttt tgcatgatct
    14641 gttctactat gattggcgta cgacgaaatt tgatgaaggc acacatgcgt atatccatcc
    14701 aagaatcgct gtaaagaatg cagaaaagat caccgatttg tcggatcttg agcgagatat
    14761 cattttgaag cacatgtggg gcgcaacgat tgcaccgcct aagtacaaag aaggatatat
    14821 cgtaacattc gtagacaaat actgcgcagt caaagaagcc gctcagccta tgtcagcaag
    14881 tatgcgtaag agatggcaac gttacttcgg aaaagaatcc tcgatttaac aggtaaaacc
    14941 aataatagac catattgagg ttgtgacaga agt
//
LOCUS       DBNICI010000060        14842 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.060, whole genome shotgun sequence.
ACCESSION   DBNICI010000060 DBNICI010000000
VERSION     DBNICI010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.060"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            120..809
                     /locus_tag="AC1ZCC_001494"
     CDS             120..809
                     /locus_tag="AC1ZCC_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629754.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            815..1771
                     /locus_tag="AC1ZCC_001495"
     CDS             815..1771
                     /locus_tag="AC1ZCC_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HIQ1629755.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            1815..2327
                     /locus_tag="AC1ZCC_001496"
     CDS             1815..2327
                     /locus_tag="AC1ZCC_001496"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1629756.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(2583..3131)
                     /locus_tag="AC1ZCC_001497"
     CDS             complement(2583..3131)
                     /locus_tag="AC1ZCC_001497"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIQ1629757.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(3220..4401)
                     /locus_tag="AC1ZCC_001498"
     CDS             complement(3220..4401)
                     /locus_tag="AC1ZCC_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HIQ1629758.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(4637..6145)
                     /locus_tag="AC1ZCC_001499"
     CDS             complement(4637..6145)
                     /locus_tag="AC1ZCC_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HIQ1629759.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            6304..6495
                     /locus_tag="AC1ZCC_001500"
     CDS             6304..6495
                     /locus_tag="AC1ZCC_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629760.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            6698..7615
                     /locus_tag="AC1ZCC_001501"
     CDS             6698..7615
                     /locus_tag="AC1ZCC_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HIQ1629761.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            7608..8681
                     /locus_tag="AC1ZCC_001502"
     CDS             7608..8681
                     /locus_tag="AC1ZCC_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629762.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            8829..9347
                     /locus_tag="AC1ZCC_001503"
     CDS             8829..9347
                     /locus_tag="AC1ZCC_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629763.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            9328..9720
                     /locus_tag="AC1ZCC_001504"
     CDS             9328..9720
                     /locus_tag="AC1ZCC_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HIQ1629764.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            9796..10731
                     /locus_tag="AC1ZCC_001505"
     CDS             9796..10731
                     /locus_tag="AC1ZCC_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIQ1629765.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            10864..11643
                     /locus_tag="AC1ZCC_001506"
     CDS             10864..11643
                     /locus_tag="AC1ZCC_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HIQ1629766.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            11636..11953
                     /locus_tag="AC1ZCC_001507"
     CDS             11636..11953
                     /locus_tag="AC1ZCC_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HIQ1629767.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            12197..13807
                     /locus_tag="AC1ZCC_001508"
     CDS             12197..13807
                     /locus_tag="AC1ZCC_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HIQ1629768.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            13964..14812
                     /locus_tag="AC1ZCC_001509"
     CDS             13964..14812
                     /locus_tag="AC1ZCC_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIQ1629769.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         4791 a         2658 c         2924 g         4469 t
ORIGIN      
        1 gaaatggtgc tagcgtggct ggcaccattt ctattttttg tttcttgtat attctgccta
       61 gtattttaaa accatttcat gtaaaatgta aatcgaaaat atgttcggag gtagaaaaca
      121 tgaagacccc gctaaccatc gaaatagaat cctcgttata ccaatattgc atcgagcaag
      181 gcggcttggt tgtagaagaa gtcacgatgc ctgatgacca aggaattgta gatacgctta
      241 gctgcttgac aaaatttgat ggaagccgag aatggcgatg ctatgagctg aaagtgacaa
      301 aagcagattt tcgctctaca gccaaactgt catttgttgg acattataat tattttgttt
      361 taccaagtaa actatatgaa gaagtaaaaa ctgaaatccc tacaggaata ggtgttttag
      421 tgtatcgtcc gtacacggtt gaagaggaga tgcctgcctc tggtacattt ctgattgcga
      481 aaaaagcagc aaagaaagag cttgctgtac cggaagaagc tttgacaaac cgcttcatgg
      541 cttctttatt cagggaagta agaaaagcaa agcaaatgga gcgaggcgta aaatattttc
      601 caacagatca attgtacaaa gaattgaaaa gacgttcaga aaatcaagat ccattttcta
      661 acgaacgata ttatgaacgg tttttagagg aaatagctag tcaggcaatc aatgagttgc
      721 aagaagaagt tatcgcgctt cagcatgatt atgaatattt gaggcaacaa cagcagatcc
      781 gtcggttgcc tacagagcca ttggagtgaa aaatatgtac tatccttatt taagaggaaa
      841 acaatttgac ctattagcat taaaagaagc actgtcgcga ggattgctct ccaataaaat
      901 ccaaccagtg atcgaaccag tacgtgattc cgctacgctt aaaaatgtga tcgaattatt
      961 tcaaaaaaag caccatccaa tcgctgtgat ccaaaatccc caagtaggac aattcaaatt
     1021 attcgatcag catgtgcaca gttggacagt caaagaaaat agctctgtcg tccctgcaca
     1081 gatcttaacg ccagaaaatc tcagtgaagt actggattca cctccagcgt ttcttgtttt
     1141 cgatggacag cactacccaa aagatacaga ggtgtggaaa cagttggcag gagttgattc
     1201 gaaatttttg ataccagata ctagtcgctt tcgcatctgg ctgcccgaaa acaaaattgt
     1261 gatccgtgac tcttttcaaa cgagaaagca tgttgaaagt tatgcagata aaacagatga
     1321 cttttttagt gatgactatc tttttttcca cgcagacgga tatattggat tttctgattt
     1381 cacgatcgaa ggtagccgtt atttcgataa agggttccct agccgagcgc ttgctatcca
     1441 tctaacgtat attgatgctt acggcaatat acgcgtgaaa cattttgtct ctgacagtaa
     1501 cgactctgct aaagaccaag cattgaagtt tcttgaagca ggagataaaa tgaaaaaatg
     1561 gatcatgcgt caccatcatc aactcttgat tacttcagga atgattgaat tattggctct
     1621 ttaccagcaa caaaaattcc ctggactggg agtgttgaaa aagtggtcac ttatgcatca
     1681 tttggaactt attagtcacc tgctagatca tccaaaagat tggcttaaat attattcaaa
     1741 agttcaggaa ctatatgaag tgatccaatg attagataat gtaaatgaaa aaggaataga
     1801 aaagaggaaa ctggatgtat attcggcaaa caaggaaaga agaagtagaa gaagtggtag
     1861 aaatcattaa tgccgcagcg gatttcattg cttcgcaagg ctctcctcaa tggcaaaacg
     1921 ggcagaaacc aacaaaagaa acgattttga acgatattga aagaaaagaa agctatgtat
     1981 taatagtgga agatcaaatt gctggaacag ctgcattagt ctcaagcgtg gatcccgttt
     2041 acacaaaaat agatggtagt tggaaagaat caactggtcc ttatttatcg attcatcgtg
     2101 tcgctttagc agaaagtact cgaggaaaaa aactagcaaa accgttctta gaacatttgt
     2161 tgacgattgc agccagcaaa aatatccaag atatccgtat cgatacttat ccagctaatg
     2221 aaccgatgga aaagaccatc tattctgctg gattcactta tcaagggatg attgaatttc
     2281 cttttgaaca cggcgaaaga aaagcatatc aaataatcat caattgacta taaaatcatc
     2341 tgagcaaaga taaagacatt aacactactg aaaaaaacaa tcatatgata gccaagaggt
     2401 tgtaacagaa gtggccagct tcaaaaaaca agaaagaatt tccgaaaatg cttctcaaat
     2461 ttttgtggaa ttttagcgta ttttacgaaa tagctgcttt ctgaaactcc cttcatcgag
     2521 aggtcgtgac atagcttttg tcacgacctc tcggcttatc ctacgagtat tattcgatac
     2581 atttatatca ataatccttt ttttatttta tctgcagctt cttggaacgc tccatcgttt
     2641 gacacgacta aaagtgaggg gatagctttc aaacgtttca actgtgctaa atcttctaca
     2701 taaaccgcca gtctggctga aatctgttca gatgaatctc cacgtttttt caggcgcgat
     2761 tgcaatgttt ctttagagat cgtaataaag ataggacgga ctaccgtccc gaaatactca
     2821 tataaatgcc aaaaaccatt tgctgtcaaa acggcgtaac aatttcctac tgtcagtttc
     2881 tttttaattt ctgctttact ggaaccatat ttatgttgag catattggtc ccactctgca
     2941 aactcatttc ccttgatcat ctgattgaat gtattgtcag aaacaaaata ataatcctct
     3001 tgatcttttt ccccaggtct tggtgatcga ttcgtgtaag taatgatctt ttgttccggc
     3061 tgaaaaatag cttctgcgag tgaagttttt ccagaaccag aaggaccgat aaatacatag
     3121 caataattca ttgtcatttc tcctttgatg attgaacaag tgaaaaagct ttggaaaata
     3181 tttccaaagc ttttaaaagt aactttgctt ttttgtatat tataccagca agccagtcac
     3241 aatagcactt aaaaagctga ctaaagtcgc tgttagcaaa attttcaatc cgtgttttgc
     3301 gacaagattt cctttttctt catttaatcc cttcatcgca ccagaaatga tcccgataga
     3361 agagaagttg gcaaatgaaa cgaggaagac agatataatt gctgttgtac gctcagtaaa
     3421 atggaaatta ccatttgcta attctgtcat agcaacaaat tcatttgtaa ccagtttagt
     3481 cgccatgata ctaccggcat ccactgcttc tttccatggg attccgctga tgaaggcaag
     3541 aggggcaaag atgtaaccta ggatctcttg gaaagtgatg ccgaatatcc cgtggaagat
     3601 agcattgatc atggcaatca aagcaacaaa accgataagc atagcagcaa ccgtgatcgc
     3661 cacatggaaa ccatcaagaa tgtattctcc caacatttgg aaaaatgtct gttctttgtt
     3721 ttcttcaatg atcagctcat cttctttttc ttctaataca tagggattta caattgaagc
     3781 cagaatataa ccgccaaata agttcaaaac aagggcagtg atcacatatt tagaatcgat
     3841 cagtgccata taagatccga cgatcgacat agaaactgta gacatagccg atacactcat
     3901 ggtgaacaaa cgtttctcag ataggaaggg taattccttt tttatggaaa taaaaacttc
     3961 cgattgtcct aaaatagcag aagctactcc attataagat tccaatttgc ccatcccgtt
     4021 gattttactt aatcctagac caacagcttt cataaataat ggaagaatct tgatatagcg
     4081 tagaataccg ataatagcag aaataaatac aattggcatc aatacggaaa gaaagaaagg
     4141 tgcacttcct ttatttgcta caccggcaaa aacaaagtca acaccttgcc cagcaaaagc
     4201 aagaagatga tcaaacattt ttgccaatac agaaacaacc gctgtaccaa aagttgtacg
     4261 taataatacg aaaccaagaa caagctgtaa aacgaacatc acaattagtg gtttcaattt
     4321 gattgctttt ttgttactgc tgatcaaaaa agcaagccct aatgtgacaa ataaaccaat
     4381 aatacctaat aaataactca aataatcact cctattccaa attccgtttt attttacctg
     4441 ttttgcaaat aaaatactaa tctttagtga aattttcatt acttttttag agtgaaacgc
     4501 attcattttc cattagaaaa taaagcgaac aaaataaaag gtttaaaaag tgtaacgtct
     4561 tagtaaagtc tataaaaaaa cgaaactatc gattaagcta atagtttcgt tttcaacatt
     4621 tataagtaaa ctttttctat tggtaatata gattttcgga agggtagaca ggtgcgcagg
     4681 taatatcgat tcctagtttt ttcaatgttt gttcatcatt tttattgacc atcacagtag
     4741 agtgggcttg tgctccctca agttcagcta gtttatcata agcaacttga gcgattggat
     4801 tcgttactgc actgattgat aaagcgatca agacttcatt gatgcttagt gcatccaaag
     4861 aggaatgaag tgtctttgat ttcaatcctt gtattgtttc aagaactaat ggtgacaata
     4921 aaggaatgtc atctgcgata tgggctaaat gttttactgc atttaagata actgccgctg
     4981 acgcatccat caatgaactt gtacgcccgg taatgatgac ttcatctggt aattcgattg
     5041 caatcacagc agaaggatct tttgtaggac tttgcgcttg aatcttatct gcatagtcat
     5101 gggcagggac taccacgttt cggtcttcag gacgtaaacc gacttcttcc ataatcagtt
     5161 tgattcgatt aactgcttct tcgtcgatca atcctttttt gaagtcgcat tctgtttgga
     5221 aataacgacg gatgatttct tgtttagagg cttcttggac aacggtatca tccacgatcc
     5281 caaatcctac acgattcaca cccatgtcag taggagattg ataaacagat tctttaccag
     5341 taattttttc aataatacgt ttgattacag gaaatgtctc aacatcccga ttatagttca
     5401 cagctacttt attgtaggca tcaaagtgga aggaatcgat catattgaca tcttttagat
     5461 cgactgtggc tgcttcataa gcaatgttca gcggatgttt caacggaaca ttccatactg
     5521 ggaaagtttc gaacttggag taaccagctg catgtccttt ttggctttca tgatacagtt
     5581 ggttcaagga agtagcgagc tttccacttc ctgggccagg agctgtcact acaacgattg
     5641 gtttagttgt ttcaatatac tcattttttc caaaaccatc ttctgagacg attttttcta
     5701 cgttgactgg atagtcttcg atttctgagt gtttatagac cttgatgttt cgtctttcta
     5761 gtttattgat aaagactttc gtggcaggct gtccactata gcgagtgata acgacactgt
     5821 tgattaataa gccataacca cgtaattcat cgattaaccg caagatatcc atatcataag
     5881 taatcccata atcaccacgg atcttattac gttcaatatc tccagcatag acgcaaatca
     5941 gtatttcagc ttgatctttc aatttttgca gcaatttgat tttcgcatct tcttcaaatc
     6001 ctggaagtac acgtttcgca tgcttgtcgt caactagctt cccaccaaat tctaaatata
     6061 atttgtcata gtggttcact cgttccaaaa tatacgctga ctgctcttca atatattttt
     6121 ctgaatcaaa accaattttt ttcactaggt tccctcctta agtactaaag cattttttca
     6181 tttttttgag gattcgtcaa ctggaagagc gaaattcctc aaaaaaacaa agaaaatggt
     6241 ttaaaccaac ttgtctagat agttattcat tggtatgata gtttttgata aggaaggtgc
     6301 tacatgaaaa aaagacagaa aaagaaaaac gcatataaac actatatccg ttcgattttt
     6361 accggttatg aaagaatgct tgaagatccc gaattggaac aattgacgtt tacttatcta
     6421 aacgaagaaa cgcagctaac tcgtgatgag catcagcgca ttcatttcac aacaagagat
     6481 ctgcctagta aataaaaaat aagataaagg tcatcggatt cgattctgtc ctatcagatg
     6541 gaaagactag ttgatcgaaa aaaaggctgt accaaatttt ggtgcagcct gttttatgga
     6601 gcattgcccg ctctttttta taacaatttt ttacagaaaa tggtactatt gaagtattaa
     6661 taagtgaaaa acagatgagt aagaggaggc aagtagtatg aaagagccag atacaaatgc
     6721 aaattatgag ccagacgaac tagaggagac gaatgcggat caggatacag gacaagaatc
     6781 gtcttctttt tacgatatca aagaaaaagc aagcatgaat ctttcccaat tattagaaga
     6841 cttacaggct tttgaaaaag ctgtccgcga agaagatatc cctgagatct accgtttata
     6901 caatggccga ttgaatgaag aattaaaaag aagctcaaat gaaaatcacg agatcgatca
     6961 attgctgatg cgtaagatcc atgatcgttt tttacaggaa tttcctttta tggaacaagt
     7021 agaaaacatc tcgccaacga tgagctatta caaaatcggc acttattacc atgatcggcc
     7081 cacggtcggt attgatgcaa gtctgccaga aatttttgtt ttgcctaaga tcgatgaaga
     7141 gtgggagaaa tattcccaac cggagtcaac ggactatgac aaaaaaatca atgagctaga
     7201 cgcaaaggtg atcactgctc agacagaaat tgaacgttta gatgaacaaa taaaagaaat
     7261 caatcgacaa atgactgatt tgaacgacaa taaaggattc ttgaatcgta aaaaaatcga
     7321 ggaagaaatt caagaattag agaagaagaa gcaagtacta tctaatgaaa aattaggctg
     7381 gcttccttat attgagacgc ctgaaacaat ccagcagcaa aaagaagcat tgaagcaaga
     7441 agcaagagca gaccagcttc gtgcagccat cgtagaaaaa gaacaaaggc aaatcagacg
     7501 ctatttcggt agcaaagagg gattcggtca agcaatccat gaattcttga tgaattatct
     7561 tggcaatgag aatcctaata atcaatctaa tgaaggaggt tccgagtatg aatgaacacg
     7621 atgaagcaga atggacaaaa aatgaagaac aaacagaaga tcatgaagaa acgaacactt
     7681 cattggaaga acaaacaagt aatcctctgg atgacacaga agaaatccct atgctagagg
     7741 aaacaaaaat acacgaaaaa gaaaaagtaa ctccgacgaa tacgaatact gcagaaaaca
     7801 cccatgctta cgaaataact gaaaatcccg ggtatttaga aggaaaagag caagatcatc
     7861 aagatatcgt cagtttgatt gaaacagcgg aagcagaatt agcggattta cgtatgagaa
     7921 aagatttgat cgaatcagat tttccacaat tattagaatc atatgatcgt tttatattga
     7981 atgaagaacc aatctccacg atcggaaaga caagcctgat cgaatacttc acgtatgaat
     8041 tactgaaacc gttgaatgat attggtctgg aacggtcaaa ttcagcttat gacacatgga
     8101 aaacaagaca tttttcgatc aattatacgt tgaatggaca aaacgaatta gtcttttcct
     8161 ttgtattacc gaccatcgat caacgatacc aagtggaatc gatgagtctc ctaaacgtat
     8221 caccagagac aatggaaatc aatgtccaag atgaccgggt gctttctttg atccgacact
     8281 ggtcagtgga tcgagtattc tctgcagggc agatcacgat attcaatcac aaattgaatc
     8341 aattattagc ccatgcaaga acacttggtt ttaccgttaa tcagacatta ctagacaata
     8401 cgaagccact tcgtttgaat cttcagagcg aatttgaatt gacgaatgaa gtattggatg
     8461 atatttttat cgtggcaatg aaaaatccat cctacgatat ggaaaagatc aacgaaacta
     8521 cttatcaagt acgattagat aaaggacagt ctttaacgat aacaacaaac gaaaaagatc
     8581 aaacagtctt gtcgatctca tctggagact acccacgatc tgtcattgat ttctttatca
     8641 attacgaatt cttggtccca ttaatggtga gaaagtcctg attggataag ttggattacc
     8701 agatgtcatt cagataattc tgttccaaag gacttgcggc agttatccag aataattgtc
     8761 acaagtcttt tctgtatgta ctaaatggac gtgaataact tgctgatgaa ggagggattt
     8821 cattaggaat gtccacaaat aaaatagcac agcttagctt attgtctgct gcttgtgtag
     8881 caggtaggat cgcttttcaa tttattccga atttccagcc aatgactgct atttttttat
     8941 ttatagtact ttacttaagc ttaaaagatg cgttaattgt catgagtctt tctattgcga
     9001 tcagcagttt ttattttggt gttggaccat gggtcatcgg ccaatggatc agctatactg
     9061 ttgttctgag tttgttttca atcgtctgct tgagaaaaac agtgcaaagg aatctatggt
     9121 taaagggagt ctgttttttt ctggcgggca tggtttacgg aataggtatg accgtttttg
     9181 atacgctgtt atatcgtttg cctcaacctt ggatctatta ccttcaaggg gtgtcgtttg
     9241 atttgatgca cagtataggc aatgttgtgt tctttcttgt ttttctgcca gtcgtcaaac
     9301 gtttttacaa tcattcagga ggaaaaaatg aaaaaaataa tttatagttt gcttgcaatg
     9361 agtgtcagta tactcatttt gacaagttgc agtacaacct atacacaatc ggcttccaac
     9421 caaaaggagg aagtaaaaac atcgcaaaaa gcaacaatca cgttacaaga aaatggtcaa
     9481 aacttttcgg aaaaagaagt ggtcttcaaa aaaggcgatg atttactgag tattttgaaa
     9541 cgaaattttg aagtcaaaga agataacggc ttcatcactt cgcttgaagg gcactcacag
     9601 gatgagaaaa atcaactcta ttggatgttt actgtggacg ggaaaagtgc tactactggt
     9661 gccaaagaga tcaaattgaa tgatggacaa tcggtcattt tcaatttgtc taaactataa
     9721 gtttcataaa aaactgttaa agggcgaata ttgccataat tcagtgagtt cagttgatga
     9781 aagaaggaaa ataacatgga aaattttaaa aaaagcggaa tgttttccgt aattgctgct
     9841 ctaggggcta atattctagt tgctatcagc aaatttgtcg gctatgcaat cagtggcagt
     9901 gccgccatgc taaacgaaag catccacagt atcgttgatt gcagcaatca gattttgctg
     9961 ttattcggtg ataaacaagc caataaagga cagagcgagt tgcatcaatt tggtgaagga
    10021 cgagcaaaat actttttcag tacaatcgtc gcaatgatgc ttttcttcgg cggtggggca
    10081 cttggggtga tggaagcttt tgaaaagttg acgcatccat cccatgaagt gggtaatccg
    10141 atcattgtta tagcaatact gttatttggt ttggtaatcg aaggaagttc gttacgcgtg
    10201 gcaatgaaag aaatcaaaga attgaatact gaaaaacttt ccacgatgcg ctttttgcgc
    10261 gaaagtcgac acagtgaaat actgattatt tttacggaag acttgtgtgc tgtaatcggt
    10321 ttggtcttgg ctttagcagg aacaatattg acttatgcaa ccgggattgc tgcatttgat
    10381 gcgatcagcg gattgctgat cggtttcttg ctgatgggag ccgccatttt cctagcaaaa
    10441 gaattttata gtctgatcat cggtgaaagc gtgacagcaa gcgatttatc aaaaatcaag
    10501 ttagcttttg aacgtcctga gatcagtcgg ctgatcgata taaaaacagt tcatttaagt
    10561 ccaacggaga ttttagttgc agcaaaagca gatatcgttg gtaacaaaga gaatgaagct
    10621 tactcggtca tcaatgatat tgaaaaaaat atgcgtcagt ctcttcctga caagaagatg
    10681 tatatataca ttgaaacaga taaatatgac ccaaattatt ctcgtaaata aaacgtttat
    10741 tcatgaattg aagcaaaaat gagaaaaata taatcttgtt tttaaataaa acggctacaa
    10801 tttagcgaat ttatgttaca atgagtccgc tgtttatttg ttgtaattaa aggagatatt
    10861 ttaatgaaca aaaagaaaag tgcgccatgg cttcaagctt tgcatgcagt gatgccactt
    10921 tgtataagct atataccagt aggattagct tgcggagtat tgttgcaaaa agttggattt
    10981 gatcctctct tatcaggact gttatcgatt ctggtctttt ccggtggcgc gcagttttta
    11041 gttgcatcta tgcttacgac gcaagcatca tttgcaacga cattattgat ggttttcttt
    11101 ttagaattac gctatacatt attaggagca agtcttgccg gttttatgaa aaaagaaaag
    11161 cggacgtttt tggctgtctt ttctcaatca ctgaatgatg aaaactatgc ggtgaactac
    11221 ttaaagtttt caactgaccc ttcctggaat aaaagaaagg ctttatatgt caattggttc
    11281 tcaatgactg catgggccgg tagcaatatg atcggtaatt ttttcggctc ggtgattcga
    11341 gtagatgctg accttgtcca ttttgctttg acggccatgt tcattttcat gtttatcatg
    11401 cagatgaaaa gcgcactact gatctttaca ggtttattct caggcgtatt aggggtcgta
    11461 ttcatggtat tattccaaaa tactttcgga ttgattgcag caacagtcat tgcttcattt
    11521 gccggattta tgctggagaa ggcattcaaa aaagaaaaag ctaagaaact aagagaccgc
    11581 ggtaaagatg tcaatgagcc gctcttcaac ttcccggaac aggaggccaa tcaccatgac
    11641 taaactatat ttgctacttt tagttgcttg tctgttcatc gtggcatata tcccgcgctt
    11701 gttcccgatg ctttatttta ctcatcgaaa agtgccatcc tggttcagtg actggatgaa
    11761 atatgtaccg gtggcattat ttgcggcgtt agcttttaaa gatgttttca ttactcatga
    11821 acatctggat attgcttgga atatcaaaat agctgcaatg attctagtag caggcgtggc
    11881 ttacaaaaca cgttcgatgg cgttgtcagt tttgacagga cttgcgtctg tctttttgct
    11941 gtctatgctt taaactgttt tattttatta aaaacagaga ttgcgacata agttgttcaa
    12001 actgatctat ccgaactgca tttgcaacag tcaattctgg atgcaagttt attcgtaggg
    12061 ataaaaaaga ttagtgttgg cttatgatgc aagctctttt ttattttagg aattagagag
    12121 ggatttgtcc ttctatgatt ttttgctata atagggaaga tgtttatttt atgaaagaat
    12181 agttgaggga atttaaatga ataaaaaatt gatgcaagga acattttggc taacctttgc
    12241 taatcttcta tgtaaagttt taggggtagt ctatttgatt ccctggctaa gtatgatggg
    12301 gaacaatcaa gatggtatgc ttgctacgac attgtataat gttggctatc ttccttatgg
    12361 attgttcctg atgcttggta cagtcggatt tccaaatgcg attgcaaaaa aagtagctgt
    12421 agccacaaaa gaaggagata acgctgcttg tcggctgatt tttagaagta cgatcaatat
    12481 catgtttgtc atcggtatcg cctctgctgc actgatgtat ttatttgctc ctttactagc
    12541 cggaatcagt ccgattgcta acgttaataa tgggatacta gctatcagaa gtttatgccc
    12601 atctttgatt gctatcccaa ttcttagtgc aatgagagga tatttccaag gaaaaaatga
    12661 tctgcggcct tatggaactt ctttgattat cgaacaggct gtgcgagtga tcgttatctt
    12721 ggctggtact tattacttgc gcgtattgac tgacggaacc atattagaag ctgtattgat
    12781 cagtacagtc gcttctttct tcggtgggtt agctgcgatt atccatatgt atatcgtcgg
    12841 acgcagaaaa gacttcttcg aacttaaaga ttttttgatt tccagccgtt atttcgaaag
    12901 agaaaaccga tctgcctctg tctctatcat tcgggaaaca ctaccgttta tttttgtcgg
    12961 ctcagtcatt actatcgttc aaatgatcga ccaagtaacg atgaaaccac tgcttcactt
    13021 tttccgtcca gagatcgcag atcagcaact ggaattctta ttcagccgtg cttctgtcaa
    13081 tccaaataaa ttgacattga tcctgatctc catggtagga acagtagcta taagcagctt
    13141 gccaatatta agcacgatga agaaaaaaga tcggatacaa atcgaaaaaa cagtaggaga
    13201 tagtttttcg attgcgttat tgattcttct tccttcattg actggaatgt ctttattagc
    13261 tggaccacta tacacattgt tttttggcta tgatcctgaa agtgtcggat atttccaaat
    13321 ggctttgcta gcttctttgt ttttctcttt attcacgatc ctatctacga tgcttcaatc
    13381 tttgaatcac catcgttttg cgatccgatt aactgtagaa gcaattatcc tgaaagtcat
    13441 cttccaagta atcggattag gattagttgg cggatacggg atgagcttat caaatggctt
    13501 agcattcggt attgtctttg ttcgcggcta tcactttatg tgcaaagaat accgaatagc
    13561 acctttagca aagatcagca attttttctt aaaaactttc cgctccaccc tgattatgct
    13621 tgcagtatgt tttgccgttt tctttttgct gaatcagcga ttgtcaatgg aatcaaaaag
    13681 ctatgcaatg atttattgtg tggctgtagg aagtattggc ggcttagtat ttttattttc
    13741 tcaattcgga aaaaatgggt ttcgaatgtt gaaaaatgtt aaacatcata gcagaagcaa
    13801 agaataggtt aactaatcgt tttttgctgt cacttgaaaa acggcaactc gtatgattac
    13861 gcttgtcgtt tttctatttt ccaattttag tttcatatct aatcttactt atgatatagt
    13921 tataaggtct ggaaaaattc tttgaaacga ggaaattaaa cacatgaaac ttgcagtagt
    13981 tacagatagt tcggcatatc tgccggaacg tatcaagaac catcccgact tattcgttat
    14041 tccaatccct ttgattatgg atgggaaaat ttataacgaa ggaatcgata tcgaagcgga
    14101 tgaatattat ggacttttaa aaaacagtaa agattttcct actacttcac aacctgtcgt
    14161 aggggaagtt ttgggtttat atgaggaatt gaaagagaaa gggtatgaca cgattttaag
    14221 tatccatctt tcttcaggga tttctggctt tgtcaacaca ttatttgcga tgaaagatga
    14281 catcagcgga gtaacggtca ttccttatga ctctaagatc actagtatgc cgatgggaca
    14341 catggtagaa gctgccttag acttaaatga tgaaggtcag tcccttgatg aaatacttgc
    14401 acatatcgat cgaatccgag ataataccta tgcttatctg attgtagatg atttgaacaa
    14461 tttagtacga ggaggacgtt tgacaaatgg tgcagcgctg attggcagct tattaaaaat
    14521 caaaccgatc ctgacatttt ctgaagggaa aattgtttta ttcgaaaaaa tccgttcaag
    14581 taaaaaagca tttgctcgag ctgaagaaat tattggtaga agaaataacg aaatacaacc
    14641 gccagtcaaa ctttacgtga tacatgcaaa taatttagaa gtagcgattg cggagaaaga
    14701 gaaattgcag aaaaaatatc caaatgcaga aatcgaaatt ggtcatttcg gaccagtcat
    14761 tggtacacat ttaggtgaaa aagccatcgg cattgccatt tcagcgcaat aattcctttt
    14821 attcaaaaaa cataccaaaa at
//
LOCUS       DBNICI010000061        14832 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.061, whole genome shotgun sequence.
ACCESSION   DBNICI010000061 DBNICI010000000
VERSION     DBNICI010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.061"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(23..1717)
                     /locus_tag="AC1ZCC_001510"
     CDS             complement(23..1717)
                     /locus_tag="AC1ZCC_001510"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HIQ1629770.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            complement(1774..1902)
                     /locus_tag="AC1ZCC_001511"
     CDS             complement(1774..1902)
                     /locus_tag="AC1ZCC_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HIQ1629771.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            1901..2149
                     /locus_tag="AC1ZCC_001512"
     CDS             1901..2149
                     /locus_tag="AC1ZCC_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629772.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2341..2706
                     /locus_tag="AC1ZCC_001513"
     CDS             2341..2706
                     /locus_tag="AC1ZCC_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HIQ1629773.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            2703..3068
                     /locus_tag="AC1ZCC_001514"
     CDS             2703..3068
                     /locus_tag="AC1ZCC_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HIQ1629774.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(3160..4587)
                     /locus_tag="AC1ZCC_001515"
     CDS             complement(3160..4587)
                     /locus_tag="AC1ZCC_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1629775.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            5057..6892
                     /gene="tdc"
                     /locus_tag="AC1ZCC_001516"
     CDS             5057..6892
                     /gene="tdc"
                     /locus_tag="AC1ZCC_001516"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HIQ1629776.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            7197..8675
                     /locus_tag="AC1ZCC_001517"
     CDS             7197..8675
                     /locus_tag="AC1ZCC_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIQ1629777.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            8641..9972
                     /locus_tag="AC1ZCC_001518"
     CDS             8641..9972
                     /locus_tag="AC1ZCC_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIQ1629778.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGRCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            10086..12638
                     /locus_tag="AC1ZCC_001519"
     CDS             10086..12638
                     /locus_tag="AC1ZCC_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HIQ1629779.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(12645..14084)
                     /locus_tag="AC1ZCC_001520"
     CDS             complement(12645..14084)
                     /locus_tag="AC1ZCC_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1629780.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            14059..14241
                     /locus_tag="AC1ZCC_001521"
     CDS             14059..14241
                     /locus_tag="AC1ZCC_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629781.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            14430..14792
                     /locus_tag="AC1ZCC_001522"
     CDS             14430..14792
                     /locus_tag="AC1ZCC_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HIQ1629782.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
BASE COUNT         4663 a         2483 c         3036 g         4650 t
ORIGIN      
        1 cagctttttc acaacctctt gattattcaa acattttttt tgcggcacga gctagagtag
       61 gaatatcttt gtcgtaacgc ggtgttttga caagacggga cataggaata cccgcaaaat
      121 ggaaagcagc gatttctcca gaacgtacag cactttgttc agtaaagatc atctgataag
      181 gctgctcgac aaattctcct gtaaaggcca gattggtcga atgtttagga atgacttttg
      241 gtcgatcgct tttggcacga ttattgaaca acgcggaagc ataaggcata tagacaggga
      301 tattatttat tacgctgtcg aaaatttctg tttctttttc acgaatattc actggtccag
      361 gatctacttt tgaaagttgt ccaatcagtt cttctagcat ttcttttcca ttcattttaa
      421 tatactgttt atccacaaat tcgccacgtc gtcttggata aaggaaataa ccccaaataa
      481 ctgtttcatt tggtttctga gacgtaaaat gcggttgatg atgcacgacg atagacatat
      541 tgacatcttt ttggcctaac ggcgtgatcg gtgtggttga aataaatgaa ttcaaggcat
      601 ttcctggttc ttgagtcgtg atacgggtga tttcattcaa caacaaatgg tttttcgtcg
      661 tcagggtaaa gctgacccat tcgcttgcat ctcgatcagc aaagaatttg tcaggatttc
      721 ccaagttgta gaaacgttca ctggcttttt tccagagact agaagctgca ccataatcca
      781 tattttctac agctggtgtt tcgtaatctc ctaaagtagc tgaatctgta atggacccat
      841 tagtgaaaat aactgctgta tcttcatcga ccacaacgta ttcttcttct tgagtcagtg
      901 tattggtaat tttcaaacca gtcacagtga tttcatcttg catcggtgtt tctttgaatt
      961 cccagtcagt gacacggcgg ttcaaaatga ttttgcatcc ttgttctttc aagtactcga
     1021 tcaaaggaag cataatactt tcgtattgat tgtaacgtgt tcgattgacg cccactagat
     1081 gttcgatttg agtgaattca taaatcattt gatgcatata gcgacgcaat tcttgagcag
     1141 aactacgtgt tctgaaagca aatgttgttt cccacataaa ccagaagttt gtttcaaaaa
     1201 agtgtggatc atctttgaaa tattcttcga tagtgatatt gtctagtttt tcttcttctg
     1261 aatcaggcat agcaatcagt ttagtcagaa gaatacggtc tttattattg aatcccagtt
     1321 ttccgccatc aatgattcct ttgccacctt ccatcaaacg tgctttgtca aaggttcggt
     1381 gttttgcatc aaaatctctc gtatcttctt cagcagtcat ccctggctca gtaacagaag
     1441 gtattcgact taacagatcc atcaaatcta cataggtacg gtaattaagc atgcgaccgc
     1501 ctcgagcaat atagcctttt tggttttcta atggatggtt tttattccaa tattcatcca
     1561 ctacagtgtc ggtcggtgca ccatcatttg aaccgtgatc atccaatgaa taaaaggtga
     1621 tctgatcccc tttccatttg ccttcttgaa tcaaataaac agccgctgcc atattggcaa
     1681 taccggcacc aatcattatt gctttacttt ttctcattgt gattcctcca atgtcttcca
     1741 agtatgtccg tagtgattca actaaatagg atgctagatt gattcgaatt catcatataa
     1801 atgactgtca tcttcgaata gtcctgtctt ttttcctaga tatgcagcgc caagggcaag
     1861 caaacctaaa ccgatgccga ctttgattaa ttttttactc atgaaaaacg cctccttgtt
     1921 ttctctttct atatccaagg atagttcgtt tttattagaa tgccaaatga taggacgaga
     1981 agaaaaagga aattttgaca aaaaagaaga agtgtctgat tttcctgtaa ataaaaatag
     2041 ggtaggtaag aagaaaagat ttgaggaggt gtatggttct atttttagaa atttgggtac
     2101 aaaaaaagca ccgagacagt gcttctttga aaagctgatg acggactaag ccttatttta
     2161 acttgctacg ttgttttgga tacttccacg atttatggat ggagggagaa aaatgggaga
     2221 gtgaaaaaga aaaatttaag cgaaccgtta tcattagaac aacttttaaa tagacgaaca
     2281 aggtaacttt cttttacaat aaaagaatag gaacaaatta aaagaaagta ggtagcgtat
     2341 atgtttcgct caaatttaat tgaatttcta gaatggatca ttgctatttt aaatatttgt
     2401 tctatcggta tcttaattgt cgggatcgtt cttgttctat tcaaactttt tgattggtct
     2461 catgtcaaga aaaactatgc agaacgtaat actcgtaatg cagatacaag aaaactgcta
     2521 gcaagttata ttttgttaag cttggaagta ctagtcgttg ctgatatcat cgagtcagtt
     2581 atcaagccga cttggacaga tattttgaag cttgcactta ttattatcat tcgaacagtc
     2641 atttcttatt tcttgaatca ggaaatagaa gacaaagaaa aacaaagaag aggagaggta
     2701 ggatgacgac agagttgatg cagtttcttc ttccatattt cgaaaaactt gtactgggac
     2761 ttgatatctt tgctatctgt ttattggttt ggggtgtttg tctatcaatc aaggattttt
     2821 tgaaatattt atttgtccat acaaaagtgc aaagcgaccg agtcagcgag aacaatgcta
     2881 ttaaaaagat gttgggaggc tatatacttc taagtttgga aattcttgtt tcagctggga
     2941 tcatcgaatc gattatcaaa cctaccttgc aggatatttt ccaactagcg gcgttggtag
     3001 ttatccgaac catcatttct tattttttaa ataaagagat cggacccgta gatgtccaaa
     3061 ataagtgagg ttgtgagcct gacgtgtatc aagagattgt tacagaaatg ggcatcttca
     3121 acaaataaga ggctgtgaca tgatttttgt cacagcccct tatttgtttt gcacagctga
     3181 aatgatagct cgatcgatcg tattgatcga aataggaata atattttcat attggaccgc
     3241 atctggtgta tataatgtac gttcacataa tacccggttt gaggactgct gcgcatactt
     3301 ctttatatca gatatacttg aataaattgt tggtgcaact tgaaaatcga aagtagttac
     3361 agcttccaag gcttcccgat aaatcagata atgaacatcc gaatttgtga cgatatagat
     3421 agtcagatgt tcttttgtca gacggagaat tccgctcaat tcgatcacaa aagcccgcat
     3481 gcaattagga ttcaaccgta gatcataagg taactggctg gaccagctgg aaaaaattgt
     3541 ctggacttct ttatataaat ttgtctgttc ctcatttaaa agataaaatt tttctggcat
     3601 gaataattga tagttatccc aagccgaacg catcagatga atcagcgcac gttcgaacgg
     3661 ttggtttccg aataattctt gattgaagtg agattcaaag gtagcaacta gttcttttat
     3721 ttcgggagta ttgtcaataa acacttggtg tagctgttga aagtcttccg caatcaactg
     3781 ataagaatgg aaaccataat tacaaagatt gaatagtgtc aaaataaagg cgaattcttc
     3841 ttttttataa taggtttgga aatcagtgtt ttcccatgct gattcgacat aattgtaaat
     3901 aggacgtttt ttgatttctt cgataaattt tgaatctatt gtcacagcac acacttgctg
     3961 gcgtaggaaa ctgatagcaa aaccaatcga taagatttct ttattttctt tatcataaaa
     4021 tctgccacta ttttccgtta cggattcaat gaaacgatcg gcacgttcaa aaaacgattc
     4081 aggtagctcc caagaattaa aaccgcctaa acgatacgac acattaagaa gcagcagacg
     4141 tctttccatt tcagtgaatc gaggggagta gcggtcgatt tcgatcccgc agtcattaaa
     4201 ataagctaat acttgttttt ttaatgaata agctttgctg acagagatga attcacattc
     4261 agttaattga agataatcag gttcatcttc ttccaaataa agcagacaag tttttaaaaa
     4321 caacgaattt tgagacaatt gctgcatgat cttgcatcga ggtatatgtt ctgaaaaata
     4381 taaagtgtaa cggtcctttt cccgatgtgt ataagcgatc tgttcttcaa attctttttt
     4441 tatgttattg atatcattga gtaaagtggt tttgcaaaca tgcaggagat tggagagctt
     4501 ttctagattg acttctttat ttacatagaa ttgcccacat aaaaacagct gtcttaaaat
     4561 atttttctca atatattctt caatcatagt agagcctccg ccttgtattt ttttgcacaa
     4621 tatgggaaat attatgacat gttttctttt tgaaatcatc agttaatttc tgaaaaagca
     4681 tataaatgcc gttaaaaaag gttttttatg ttataaaatt tggaaaacag ataattctat
     4741 taaaaaataa attgcgaatt tttaacaata taaaaaaata aaaatatgtt tagtgaatga
     4801 aggacagtca ttttatttgt ttgttcgctt tttttaggtt atctatttta gaagactgat
     4861 ttattgaaaa attgttcgct tttttgcgtt gaattatcag aaaaatccga ctttatttat
     4921 ttttgtttgt aaaaatctac gtggatttga tgttataaaa aaaaaattct catttttggc
     4981 gctatagtga agaggtgtta cagataaaca catgtatctg caagacattg accaacaaaa
     5041 tatggaggga agaaaaatga aagatatgga tatcaaggcc gtctttatag gcgacaaagc
     5101 tgaaaatgga ccagtttata aaatgttgtt gaacaagatg gtagatgaac atcttggatg
     5161 gagagaaaat tatattcctt cagatatgcc tgcaattagc gaaggagata agctcactcc
     5221 agattatcta gccacccgcg accatatgat agaggtcttg gatgaagtca gtcagcgatt
     5281 acgcgcagga tcgatcccat ggcattcagc aggccgttac tggggacaaa tgaatgctga
     5341 aacattgatg cctgctttat tagcttacaa ctatgcgatg ctgtggaacc caaataatgt
     5401 tgcattggaa tcttctatgg caacttcgca aatggaagca gaagtaggac aggattttgc
     5461 ttcattattc aatatgacag acggatgggg acacattgca gcagatggtt ctattgctaa
     5521 tcttgaagga ttatggtacg cccgttgcat caaatcgatt cctttagctg tcaaggaagt
     5581 gctgcctgaa aaagtgaaaa aaatgtctga gtgggaactt ttgaatttat ctgttgaaga
     5641 aattttggaa atgaccgaaa gctttacgga cgaagaaatg gatgaggtca aagcagcttc
     5701 ttcccgtagc ggaaaaaata tccaaagatt agggaaatgg ctggtccctc aaacaaaaca
     5761 ctattcttgg atgaaggcgt tagatatttg cggagtcggc ttagatcaga tggttgctat
     5821 cccagttcaa gaggattatc gaatggatat taatgctttg gaaaaaacaa ttcgtgaatt
     5881 agctggacaa aaaatcccaa ttctaggtgt ggttgctgtc gtaggaacga cagaagaagg
     5941 tcaagtagac agtgtagata aaatcgtcca attaagagaa aggttaaaag atgaagggat
     6001 ctatttctat ttacatgttg atgccgcata tggcggctat gcgcgttcac tgtttctaaa
     6061 cgaagcagga gaatttgtgc cgtatgcttc cttagctgaa ttctttgaag aacatcatgt
     6121 tttccaccac tgcgtaacga ttgacaaaga ggtatatgaa gggttcagag ctatttcaga
     6181 agcggattct gtcacgatag atcctcataa gatgggctat gtcccttatg ctgctggagg
     6241 aatcgtaatc aaacataaaa atatgagaaa tatcatttct tatttcgcgc catatgtgtt
     6301 tgaaaaatca gtaaaggctc cagatatgtt aggagcttat attttggaag gatcaaaagc
     6361 tggagcaacc gccgccgcag tctggacagc acatcgtgta ttgccattga atgtgaccgg
     6421 atatggtcaa ttgattgggg cttcaatcga ggcagctcaa agattcagag aatttctcga
     6481 tcatctgact tttactgtta aaggaaaaac aatcgaagtc tatccattga atcatcctga
     6541 ttttaatatg gtcaattggg tattcaaaga acaaggctgt acagatttga acgctatcaa
     6601 tgaattaaat gaaaaaatgt tcgatcggtc ttcttatatg gatggcgatg tttatggtga
     6661 acggtttatt acttctcata ctacatttac acaagaagat tacggtgatt ctccgattcg
     6721 ttttgttgaa agaatgggtc taacgaaaga agaatggaaa aaagaacaga aaatcactct
     6781 gctacgtgcg gctatcatga ctccttattt gaatgatgat cgaattttca atttttatac
     6841 aaaaaaaatc gccaaagcaa tggaaaagaa actaaacgaa atcatccaat agaactgatt
     6901 tttgtgtgaa agaataacaa gagacatatg gagaacagac aattatttgc ttcatcaaaa
     6961 caattgtatt tccccctgtt tttcatatat tcctcgccaa gatattcttt tttataaaac
     7021 gttctataag ttggatcgtt cgtttcatgt atcaaaccca gttcactaag atcgttcaat
     7081 ggatgtttcg gtgatactgg gttttgtact gaatcgatga aaccttgtca tttctgttct
     7141 tgaacaaaaa taaaagaaaa caaaaacata tgtcgctatt gaaagaagga ataaagatga
     7201 aagatactgg ttcatcaaag caaatcacga ccatgacctt tatcggaatg acctgcgccc
     7261 ttgtagcaag tgtacgaaat attccagatg tagcagcaac gagctggacg atgatttttt
     7321 acatgctggt tgcagtatta ttgtacgctt ttccaatctc attgatctct ggtgaatttg
     7381 ctggaatgtt tccccaaaaa ggtggtccag aactttgggt atcgaatgct ctaggaaaaa
     7441 aatgggggtt cgttgtctca tggctgttat gggtccaaat gtttcctggg atggtgatgg
     7501 tcgcttcagc actcgctccg ttgtttggta atattatcga taatgtgccg cttggtttga
     7561 acagcaaatt tacattagtt gtcattttag tcgtttattg gatcatcact tttttgaatt
     7621 tgaaatttga tatggcaaaa atcggcggaa aagtcggtgt ttggttagga ttgtacgtgc
     7681 ctttgacgat gatgctctta ttagggtttg ctgcgtggat caaagttggg atcgtaccaa
     7741 ccggaacatt aggcagtttt tcatgggaca aattaatacc agatactacg actgcatcct
     7801 catttgttta ttttgcgccg atcatgttta tttttactgg tatcgaaatg tcctctgtgt
     7861 atatcactcg attggaaaat ccagttaaaa cgtatattcg cggagtattt gccgcgttaa
     7921 tttttatatt tttcgtcaat attttgaatg ctttagttgt agcaaatgtc gtaccaaaag
     7981 gacaaatgga attgaataat atcgcgcaat ctatttcgat ttattgccaa attcttggtt
     8041 tgccacatct tattgtgaat ctgtttagtt tacttgtctt cattggtgta gcagtccaat
     8101 tatctgcttg ggcctcagga ccagcaaaga cagtgacaga aagtgctaga aaaggtgcgt
     8161 atccaccaaa attcaatttt tggaagacga atcaatttga cgtatccaaa tccgttattt
     8221 tgactcagtc tgtcattatc tcaatctttg cactttttta tttgctgatt cctggcgtca
     8281 atcaagcatt tttgatgctc gtaaattcaa ctactgttat ctactgtatc gtctatgtca
     8341 tcatggggat cgcagttttg agattgcgtt atacacatgc aaagttgaat cgaccattta
     8401 gaattggaaa aaaagagaaa aagagcaatc ttggagtttg gactgtcgtt attgttttat
     8461 ttgcggcaat cggattttct gtcggactga cgatgaaagc tggaacatgg atcaatctaa
     8521 ttgcagtcac agccatcagt gtcattttgt ttgtagtacc actctgtatt gaaaaaataa
     8581 agaaaccttc ttgggaacag gaagtcgcca tgctgcttga aaaagaaaaa ggtgaacttg
     8641 atggaaaaac cggcaatgaa acggactcta actaatcgaa atatccagtt aatcgcatta
     8701 ggtggcgcaa ttggaacagg attattttta ggttcagcaa aggcaattgc atcagcaggg
     8761 ccagctgtta ttctcgttta tttgatatgc ggtttggcta tttatggttt gatgcgggcc
     8821 atgggtgatt tatttttagc aaatagtaca tttaactcta tcagtgattt cgttgactat
     8881 tatttaggca aaaagtgggc attttttgtc aattggactt attggctttg ctggcttgga
     8941 gctggacttg cggaattaac agcaatagga ttgtatatcc atttctggtt tgtacatgta
     9001 ccagctttga taagcggagg tagcgcacta atgatcttaa tggcagtcaa ccttgttgca
     9061 gtcaagtggt ttggtgaatt ggaatctttt ttctcaatca tcaagatctt ggggattttc
     9121 ctttttatct tattgggtat aggattgagt gtctcagatt ttcataccca gtctatcgga
     9181 atcaaagaaa attttggaca gatcgatgaa ttactaccgt ttggctttgc tggaatggta
     9241 gtctcatttc ctatggtttt attcagtttt gctggaattg aaatgatcgg cttaaccgtt
     9301 ggcgagacaa gtgatccgca aaagaatttg aaaaaagcaa tcaactgtct gccttggcgt
     9361 attctctttt tctatatagg aacgattttg tcgattttat ttgttgttcc atggcattgt
     9421 ttagatttaa aagaaagtcc tttagtgact atgctgcaag tgatccattg ccaaggcgga
     9481 gcagcaattg tcaatctaat gattttggtt gcttctttgt cctcagcaaa tagtgcagtc
     9541 tacagtacta gcaggatttt atatcaaaca gcaaaagaaa aaagtttgcc ccattggttt
     9601 tgtgcgcttt ctaagaaagg cgtaccttta cacggtattt taattacagg aatgttattt
     9661 tttgccggtc gttgtctaca tttttttgta gctgatagtc gattgttttt tcagcttttg
     9721 gcaggcatga caacgagttg ttttctattt gtgtggtctt cgatcttgtg tgcacatgtt
     9781 caatttatta aagaaaagaa acggaacact acacaaagat ataaagatct tgctttgttg
     9841 gtgtttttta cttcaattgc tttcagcttg ttattcgaaa gcacgacacg ctttattcct
     9901 tttttattgg ttatctggtt tgtattctta aattttgtat ataaaagaat aagcaataga
     9961 aatatcagtt aggcccagat acattttgca aaacgaatta tttcagaccc ccctgaaata
    10021 atcgatacgc ctgaaaaaac ttccctgcgt ataataacag tatcaattaa acaggagggg
    10081 ataggatgaa ctggtacaga ttgacggtag aagatgtatt gaaaaactat caaacatcaa
    10141 cagaaggctt gtcgcaagaa gaacgacaga aacgcctgac tgatcaagga tataatgaaa
    10201 taaaagcaag gcagcagaca aaacgatgga aaaaaatagc aaagcatttt actgatctgc
    10261 tgatgatcgt gttgatcatt gcctcttttt tgaaatttgc ttctagtgaa tatattgaag
    10321 gaagtattat tttattcgta gtgatcgtca atggactcgt tagttactgg caagaaagaa
    10381 aagcggaaga atcattgaat ggtttgaagc agctgatggg acaagaagcg atagtactat
    10441 caaacgggat gcaagaaaaa atccctgcga aagaactggt aaaaggagat gtcattgtct
    10501 tacgtgccgg agatgtcgtt ccagcagata tccgattaat cgaagcacat gatttattag
    10561 tggaagaatc gattttgacg ggagaatcag aagcatctga aaaaagtcat gccgttttga
    10621 cagaagaaga atcgatcggc gaccagaaaa atatgggttt ttcaggaacg cttgtacaat
    10681 ctggttctgc agtcggtgtc gttgtagaaa caggaaacac gacagaaatc ggtaaaatca
    10741 atcaagcatt gcaatcggtc gaacaacaaa cgacgcctct gattaaaaaa atcaaccgat
    10801 taaacaaaca aatttttcaa ggcattcttt gtttgagcct attcttagtt atttttacga
    10861 catttcgata tggtatggat tggcatattc ttctttcggc tacgattgct ttggtggtgt
    10921 caatggtacc cgaaggattg ccagctgttt tgaccatgat tctatccgtt ggagtacatg
    10981 aaatggcaaa agaaaaggcg atcatcaaag gacttccttc ggtagaaaca ttaggttcta
    11041 tgacagtgat ctgttcagat aagacaggaa cattgacaaa aaatgaaatg acagttgtgg
    11101 atgtagctgc aaaagaagaa gcctgcgtgt tgtccattat gaaaaactgt caggaattaa
    11161 aaacaaaaga agaacagaaa acagaaaatt taagtggcaa tccgacagaa gttgcgctga
    11221 ttcgacacac agaaacagcc aatctacctt tgaaaaaaat agaagcaaag attccgttca
    11281 gttctgaata caaatatatg gcaacgatgc atgcggagga aaaaggagcg gtgatttacg
    11341 tcaaaggtgc accagaagta ttatttgcta aatcaactct ttcttctgca gaacaagaag
    11401 catggagtca gacagctgca gaatttgcga gaaaaggtca gcgtgtttta ggatttgctt
    11461 ataaaaaagt agattcgaaa caggaactca cgcatgagac tttaacccaa ttaacttttg
    11521 cagggatcgc cggattgatc gatccgccaa aagaaagtgc agtcaaagct gtaaaggaat
    11581 gccaacaagc agggatctca gtgaaaatga ttacagggga tcacaaagac acagcaaaag
    11641 cgattgcgga acaaattggc ttaaaacata ctgccaaagt tttagaaggg atcgatttgg
    11701 atcttatgtc tgatgaagag ttgattcagc aagtgccaat agtcgatgtc tttgctcgaa
    11761 cgacaccaga acataaactg cgaattgtta aagctttgca gaaaaatgga gaaatcgtcg
    11821 gaatgaccgg agacggcgta aatgatgcac ctgctttgaa aagatccgat gtaggaattg
    11881 ctatgggaat caaaggtagt gaagtaagca aacaggcagc tgatatggtt ttaggagatg
    11941 ataatttcca tacgattgca aaagccgtaa aagagggccg gcggattttg gataatttgc
    12001 aaaaaaccat caatttcttc ttgccaactg cattagctca aggattgatc ttgatttggg
    12061 cacttatgct gaatcgcccg ctacctttgt ccccagtaca gattttatgg gtcaatatgg
    12121 taacgacaat cactttatct tatgcactag ggttcgagtc gccagatcca gaaatcatga
    12181 agcgcccacc aagagatcca aagcaaggaa tcctatctgg ttatcatttg tttcggatca
    12241 tctatgtctc cctactgatc atggttcctg cctatttatt ggctatgcag tttgaagggc
    12301 aaagcttaca gcaaaccatt cttctgcaaa atatcgtctt agctcaagct gtttacatga
    12361 tcaattgcag agagttgtcc aagccgtcat tgaaccaagg tcttttgcaa aacaaagcgc
    12421 tttttctttc attaggtatc cttgctttat tgcaagggat ggttattttt ctaccagtcg
    12481 gccagcagct gatcggtaca gtaagtttaa cgatgacaca acagttattg attggagcaa
    12541 atgtgattct tctattctta gttgtagaaa tagaaaaagg gatcatgaac aaactgtttc
    12601 gcaaaaaaga aaaaacagct agagaataca gtcgctagcc gttactataa aaaagttttg
    12661 aaagtgagtt cagtcaaacg attgagctca tttttcgttg gaatagaaga aatgtataca
    12721 atagggatat ctagagaatc tagagggaaa ttggaaatca agatatctgt tttttcaaaa
    12781 gaaatagtgg aaagatcggt tagttcgata ggtaagaagc agacacgttt tcctaagtaa
    12841 tcttttaatt cctgctgcaa gaacgccttc catgaaggtt ctccttggaa aataaaatag
    12901 acattgaaca aattcggttt gatagaaaaa atcgtttgtt ggatcagtag agacaagagt
    12961 tggagaaagt aatttggttc attgatttct atttcttttt ttagatcgtt cagtaaattc
    13021 tctttctgtt gatacaagtg tgcatattgc tgatcatatg tttctaaaaa acgatggtat
    13081 tgacggtcca atgcttcaat caatatcggc gattcatgat attttaatat gaggagagcc
    13141 aagttatcag tcagagatgt tttttttagt tgatcaagag acagctctga agctagttcc
    13201 tctacaaacg tttcgaggac ctctaaaaat ggagaatgat gcaggcagcg agcggtttct
    13261 tgtccatagt tattgttgta attccaactt tccagaaagc agaaagaagc aaaagaaagc
    13321 tcagtgtctt taagataaat accttccgtt aaatacaatt ttttcaatgc agagtcatac
    13381 agggaggaga gtgtgctgtt ggtatggata gaagaatcta atcggcaatt cgcttttata
    13441 cggatcgtat tgataaagaa ccaacatgtt ccgaaaatct cttctgtttc aaccgctagt
    13501 tcagtttgac tgaggtttct taaaaattga aaataatggg attcatggat cgaataatcc
    13561 tcgaaaaaat agttgttatg agtgaaagga atcagcaatc gatgataaaa agtacgaatc
    13621 gcacgctctt ttccaagaat acaagctgta tatttcgtca aatcgatctg caagtcgtaa
    13681 cttgctaaac gctgattgat tttagtcaca tgtcttttca atgtcccata agaaagccca
    13741 tggtcggcta gaaaatcagg gacagcaact ttttttgtaa aaagcagatt tttcagcagt
    13801 tcgacactga ctgattgttt gaaaaaaatc tcccaaaggt catttgttaa taaattttgc
    13861 tggttttgca aactgattcc tgcttgatcg ctgatgatgt cccagccttc gggcagctga
    13921 gagcgaatag attgaaggtc agaaaaaatc gttcgttcgg ttgtccctat gatttcagct
    13981 aattcattta cggttatctt tgtatgattc agcaattgtt ctactaaaga aacatgacgt
    14041 cgaatatctt tttcggtaat gatttttttc attagtgaat acatcaatca gactcccttc
    14101 tattctaaga atacggagaa aataaacggt ggtcaagttt caactgattt ttcaatcatt
    14161 gtttattttt ctggagcatt tactgatgat aagaatttca tcatgaactt gaaaaatgat
    14221 accactaaaa tcaaaaaata ggtgggcaaa caataataaa cattttaata gtagatggta
    14281 gcaagaccaa cacaggcgga ttgaacattt aagagttgat aaaaaagaaa aatatcgaag
    14341 tatttgatta gcaactactt gtattcattt ttctattctt acgtggtatc tttgaataaa
    14401 gcgaatcaat aagaaagaga tggaagcaga tgctaacgaa aaaagaacta atcgatcaca
    14461 tcaatgaaac atacaatttg tctccagaat atatttttaa aaaatttcct gattattgcg
    14521 tattcagaca tcaaaaaagc aaaaaatggt ttggggtcct gatgtctgtt ccagaagaaa
    14581 aaatctatga tgatgcaagt gaccatagga tcatcaatat tcttgatttg aaggtcgatc
    14641 ctgaacttgg cggaattttg cgaggaaaag aggggtattt tccagcctat cacatgaata
    14701 aagaacattg gatctctatt gatctacgta aaatagaaaa gctagatgaa ctagcagatt
    14761 tgattgatgg aagttttaaa tcaacgcaat gaatgtctag gacaatagag aagaggttgt
    14821 gacagaaatg tt
//
LOCUS       DBNICI010000062        14818 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.062, whole genome shotgun sequence.
ACCESSION   DBNICI010000062 DBNICI010000000
VERSION     DBNICI010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.062"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(9..482)
                     /locus_tag="AC1ZCC_001523"
     CDS             complement(9..482)
                     /locus_tag="AC1ZCC_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIQ1629783.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(587..1450)
                     /locus_tag="AC1ZCC_001524"
     CDS             complement(587..1450)
                     /locus_tag="AC1ZCC_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HIQ1629784.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1646..3745)
                     /locus_tag="AC1ZCC_001525"
     CDS             complement(1646..3745)
                     /locus_tag="AC1ZCC_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HIQ1629785.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3965..5074)
                     /gene="rpoD"
                     /locus_tag="AC1ZCC_001526"
     CDS             complement(3965..5074)
                     /gene="rpoD"
                     /locus_tag="AC1ZCC_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HIQ1629786.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5098..6939)
                     /gene="dnaG"
                     /locus_tag="AC1ZCC_001527"
     CDS             complement(5098..6939)
                     /gene="dnaG"
                     /locus_tag="AC1ZCC_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIQ1629787.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7121..9436)
                     /locus_tag="AC1ZCC_001528"
     CDS             complement(7121..9436)
                     /locus_tag="AC1ZCC_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIQ1629788.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9621..10481)
                     /locus_tag="AC1ZCC_001529"
     CDS             complement(9621..10481)
                     /locus_tag="AC1ZCC_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HIQ1629789.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10540..11172)
                     /locus_tag="AC1ZCC_001530"
                     /pseudo
     CDS             complement(10540..11172)
                     /locus_tag="AC1ZCC_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            11339..12100
                     /locus_tag="AC1ZCC_001531"
     CDS             11339..12100
                     /locus_tag="AC1ZCC_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIQ1629790.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12126..14717
                     /gene="mprF"
                     /locus_tag="AC1ZCC_001532"
     CDS             12126..14717
                     /gene="mprF"
                     /locus_tag="AC1ZCC_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HIQ1629791.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
BASE COUNT         4021 a         3076 c         2578 g         5143 t
ORIGIN      
        1 ctagacactc agacggactg gtttcttttt tctttttcat agcattcctg acagattcca
       61 tggaacgtta gccgatgatc ttttactaaa aaatgataac gttcttctac tacttgctcc
      121 acttcaagta aaagatcctc ttctacttcc tcgatcgtgc cgcagttcaa gcatagtaaa
      181 tgatgatgga aatgtttggc gccttctttt ctcaaatcat aacgggctaa tccatcatta
      241 aaacttactt tatcgacaat tttcaattct gtcaagattt caagggttcg ataaacagta
      301 gcaagtccaa tttccggact cttttttttc actaaaaaga agatttcttc tgcagataaa
      361 tgctctttct cattttccaa aagcacaagc agagtagctt ctctttgcgg tgtcagctta
      421 aaccccgctt catgtagctg ctgttttgtt tttttcatcg ccgaaatagc attcatgacc
      481 atttactcaa ccccaacaat ttataataat tacaaataaa aaaagatata acactatgtt
      541 aattaaaatt attataagct agttaagaaa aacatgcaag ggattctcaa ttagattttt
      601 tgactagttt tgtcacaccg ttttcttgtg taatcaatcc ttgtttcaaa agattaccta
      661 atgcgcgctt aaatgctcct ttgctgatac caaaagcttt catgatttct tctggatccg
      721 atttatctgt aaagttgatt tgctgatctg cagaacgttc taaaatggtt aaaatcattt
      781 gtgcatcatc tgatatagct tcatatgctc ttggcttcaa tgaaagattc agtacaccgt
      841 ctggtcggac accaatcact cgacctttta tctgctcgcc tagacgaggt tcattatatc
      901 gttcagaagg atggataaac ccaagataga aatcttccgt taaaacgtac gtacctgtta
      961 atttcaagcg gtatgctgtt cctatcacat ctttgttttt catttcttct gttccatatt
     1021 tgcttaaaga tttgaatatt ttttcatctg ctaaactgcc ccaaatacgg tcttttgcat
     1081 caactctaag ggaaatcatc aagcgatccc cttttttagg ccatagctct gtcattgtcg
     1141 gcaattcatc aagagatacg acaaagtctt tatctggaag accgatatcg acaaacgcac
     1201 ctaaatcttt tcgtgtagca gtaacgattc caaaagcgta atgaccgata cggcttttag
     1261 gaatttgtgt agtgatccga ttttcatgtt tttgattttg gtaaccgaat ccttcaactg
     1321 cttcaccgat tttgtgttca ccttctgatt tgcttaagcc aaaggtttgt ccgttttttt
     1381 ggacaaagta gtttttttca ttttcatcaa taataatacc tgtaaatata gttgctaata
     1441 aatcgttcat ttttttctcc ctctatagtg aaaggttttc ttaatacatt tctttgctat
     1501 cataacataa tgttatctac ttgaatagct gagccttggt ttggaaaggt gaaaaaagga
     1561 gtatgacaac agttgtgtca tactcccctt tttcgaataa acggtgttaa acggcaggct
     1621 cacagcctct ttcatttgcc taaaattaat tgctcccatt tcctccgaac catccagttt
     1681 tacagttgat ggttacgatg taaaatggca aagcactttc tccaatgcct ttcatcaatg
     1741 cagattttgt tgaagtggca tatagattat aatagccttc cccattttcg attctggctt
     1801 tttctaatct gtaacctcct tcaacgatat atccacgttg gatacatgta gcaatcactt
     1861 tgctttggac acctggtgcc cacgtccatg ctggattaga tgcatcggca atatcacttg
     1921 ctctggcaga tttgattggc tgattcgctg agtttgaaga ggaaggggtc gaggatgccg
     1981 cttcttgctg tgctgcttgt gcgaccgcag cttgttgagc ggcttgagct gcagctgctg
     2041 cttgagcggc ttgttttgct tgttcttcag ctgctttttt cgcttcttct gcttttttct
     2101 tttcctcttc ttcttttttc tctttatcag tgagcatctt atctacttgt ttcaattgtt
     2161 cattcaactg agcagataga tctgtatttt tcactttttc tactgcttct tttgccgtct
     2221 tgtattggtc gcgtgttgct gaatccacta cttttccgtc tttaaagact atagctgttt
     2281 tttctttagc tgtttgcaat tcagcatatt gatttttagc ttctgtttgt gcttgttgaa
     2341 tcaattttcc aaaatctgtg ttatccgttt cgattgcttg gatctcttta tctgctttca
     2401 acttgatgtc atctgctaga tgatcatcga cgatggcagg tgctatgaac cactcattca
     2461 ctttttggat atctcggatc ttagattgga tatcttggta agttgtttct aactctgaat
     2521 atcctttttc attcttaaga ctggataaat cattttttag ttttgataaa tcttgatgaa
     2581 tcatatctgc acgaataaat tgatggtgat catctgtata aaaggcagca agtttctcat
     2641 gaagttcttc catttgttgc tgtctttttg cttctgctgc tacttcggct tctacttttt
     2701 gatgatggtc ataataagcc cagcttcctc caccgagtag aagtactgct gctaaaacga
     2761 gataaggtac tttttttgat tttttctttt gcgctttatc tggatctgtt ttttctaagt
     2821 ttgattcttg atgtgttttt tcagtatctg gcacgattgt tattgatttc gcttctgctt
     2881 caaacagctt aggctggttc tcttcttttt ctatttttgc ttcaagatca gtaccctctt
     2941 tgcttacaga ttgattttca gcatcggatt cactatttat ttcatcgggc tctgttgact
     3001 ttttgtctgt gttttcgagt atttcttttt tctctgcttc cggcagttta ttcgtttttt
     3061 ccttttctaa ttgtttttca gtaaatactt cagttttttc tgtgatatcc gtttttacgt
     3121 cttttatttt tactgtttct ttacctgtgg cgttttctct atcagagtct ttttcagttt
     3181 cttgttcctt ttctggctct actttgtttt cctgtgcaat tttttcactg atcattttga
     3241 gcagctcttt cgcgtgctct tcttttgtat cttcacgatg tgctttgata taagcggata
     3301 gtatcgaatt ttcttcctca ttatcgacga tttctttcag ttcagctact cttctttttt
     3361 gttttgattc aagcgtttct tcttgattct ttcctgattc ttccgaagtc tcatgaacag
     3421 ttgctttctt ctgttcattt ttctctatat tagttacttg atcgtgagca ggtggttctt
     3481 cgtcttctgg ttctgctttt tcgaataatt ccattacaga ttctagaggt aaatctttca
     3541 attccgacca ttcgattgga tcgtttaatt ctgtatcagg aaagactgca tcttcagttg
     3601 atttatcctg catagctgca tctgtatttg tttcttcaat tgattctttt atttgctgat
     3661 cttctgcagt tatttgatga ccgcagtctg gacagatagt attctgctcc atttttttcg
     3721 atccgcaatt tggacatttt ttcatcactt agcgatcggc tcctttacta ggtttacgct
     3781 accatttaat ctatcatatt ctatacgtca cgccaatagc ttatttttag gatttactta
     3841 aaaataatga atgaatttcc ttcctataga aaaaaaagct tgagactgcg tagtctcaag
     3901 ctttttttct tttaaaataa accaaacagt tttactgatt tctcttctgg tcaatagtcc
     3961 acgcttattc taagaaatct tttaattgtt ttgaacgaga aggatggcgc aattttctta
     4021 atgctttagc ctcgatctga cgaatacgtt cccgagtaac accaaatact ttccctactt
     4081 cttccaatgt tctcgtccgg ccatcatcta aaccaaaacg taagcgaagg acattctctt
     4141 cccgatcagt caatgtatct agtacatctt ccaattgttc ttttagtaac tcataagctg
     4201 cgtgttctgc tggacttgtt gcttcctggt cttctatgaa atcacctaag tgagagtcat
     4261 cttcttcacc gattggcgtt tcaagggaaa caggttcttg cgcgattttc aagatttctc
     4321 ggactttttc tgttggaaga tccatttccg caccgatttc ttctggcgta ggttctcttc
     4381 caaggtcttg cagcaattga cgttggatac gtatcaattt attaattgtt tcaaccatat
     4441 gaactggaat acggatcgta cgtgcttggt cagcgattgc tcgagtgatg gcttgacgaa
     4501 tccaccatgt agcataggtt gaaaacttaa atcctttgcg gtagtcaaat ttttcaactg
     4561 ccttcatcag tcccatgttt ccttcttgga tcaagtcaag gaactgcatg cctcgtccta
     4621 cataacgttt agcgatactt actactaaac gaaggtttgc ttctgccaga cgctgtttcg
     4681 cttcttggtc gccttcttcg attttcaatg ctaacgctac ttcttcttcc gcagttaaaa
     4741 gggagacacg gccaatttct ttcagataca tacgaaccgg gtcattgatt tttacaccgg
     4801 ttggcgcaga aagatcttct gtttttgctt gttcagcttt tttctcagca ctcttcaagc
     4861 tgtgaattga tggatcacca ttttcatcca ccacactgat acctgcgtct tcaatctttt
     4921 ggatcaattt gtccatttca tctgcattca aagtgaatgg tgtagcaagc tgattggata
     4981 aatcatcata tacaacttgt cgtttgggtt tgttttcttt gatgaaagct gctactgctg
     5041 cttcatacgt ttttttatct gttccatttg ccataaaaga aggcccccct tgagtagcta
     5101 tttggcttgt tttaactgtt tcgttaaatt gatgatttca atcgtcaatt caagctcaag
     5161 ctgtttgttt ccagttcgtc tggcttcttg ctgcatttgt ttttttaatg ttatttcatc
     5221 agctaagcca gatttgctga tcacatctag caagtcttgg atttctcgtg gcgaactttc
     5281 ttctgccacc ggtaaatatt cgatttccac caccaaccgc cttaaatttt catcttgcaa
     5341 aaagtctaaa aactctgcaa ggttgaattc ataatgttgt gagacatatg catcgaataa
     5401 aaaataaact tctgcatatg catcatgaat aaactgaaat tctcgttctt ttaaaatatt
     5461 acggacagaa acttctttaa atgcacgata taatagaatc tgctctgcct tttctacttg
     5521 cgtcaaaggt ctctttttcc gtgcagtttc ttgtctcgta acctgagtgt gttgactttc
     5581 ctgaggttct tgacgatttt gctgatttgt ccttcgaaga tcgcgcagtt gtttttgtat
     5641 cgtctctctg ccgatctgaa actctgttga cagttgagat agatacatat cttgttcgat
     5701 tggtgaaaca actcggctaa gttcaactag taattcttcg aggtattcga tcttgtcttt
     5761 ttcattatct aaatttttcc cttgttttaa ataacgtgat ttaaaagtaa agactgtttc
     5821 gcgcccatga gccactaatt cttggaaaga tgcttctcca tacttacgga gatattcatc
     5881 aggatcaagc ttttctggaa cgactacgac agaaagtgct aatcgactgt tttgccgaag
     5941 caattcaatc cctcgattgg ttgcttcaaa acctgcgttg tctccgtcat agcaaaagac
     6001 caattctttc gtcagctttt ccatttgttg gatctgttga gctgttagac ttgtccccat
     6061 agaagctaat ccagttttga taccagcctg ccatgcagca atcacatcca taaacccttc
     6121 gaacaacaaa atttctccgt ttttccggat atcgcttctt gctttatcaa gattaaataa
     6181 aacttgtctt ttattgaaca gttctgtttc tggactattc aagtatttgg gctgatcttt
     6241 ttcctgccct ttttcaggtg caagccagcg tcctgaaaaa ccgatcgtct ggccttgaga
     6301 attacgaagt ggaaacatca ctctttgata aaaacgatca gacatacggc catcatcatg
     6361 cgtgacaaac aatccagagc gttctaacaa ttcatttgaa attgattcat tcttgaatac
     6421 ttgctgaaga aaagaacgct cattaggtgc aaaacctatg ttgaattctt caattaattc
     6481 taaagacaac ccacgacttt gcaaatattc taaagctggc tgtccagctt ttgtatgcaa
     6541 tagcatgtga tgatagattt ccgctgcttt ttcatgagca gcaatcagtt tccccgtagc
     6601 agattcagga ttttggacag ctatagtacg ctgacgccat tgatcttcta acggtatctg
     6661 ttcgaagtct gccactttta ccactgcttc tggaaaactt agaccttcta gttcttgaag
     6721 aaaagtaaac acgtttccgc ctttaccaca accaaagcaa tgaaaaatct gcttgtcttc
     6781 tgcaacagaa aaagacggtg tacgttcttc atgaaaagga cacagcccca agtaattttt
     6841 accagacttt ttcaactgga catattgtcc aatcacctca acgatattcg tttgactgcg
     6901 tactgtttcg ataacctctt gaggaatccg ttgagccatt ttttcacccc ctgctctatt
     6961 gatatctttt gggtttaaca gaagatattt caggagaata accggtaaaa aatcgcacta
     7021 actgaaatca gtgcgatttt aaaggacaca attatacatg tttgattgta tcatatttca
     7081 ttgctttttc aagtataaca catttctatc tcattaagca tcaatttgct actcttttca
     7141 gtaactgatt ttgaatatca gccttccagt acaatactcg aagaacaata tttacgagca
     7201 tgaatacaaa gaagtatcct acgctctctc ccaaccctaa gtttacttcc aaaattccat
     7261 ggaacagcat tgcagctaca tatatcccta cgatcgtcgt tactgctagg aagatgcgca
     7321 atgggttgaa gggataacaa gcttttacaa ctgccatgca actgatccca atcagcaagt
     7381 aatacatcaa agtagtagtt tcagcatttc cccatgcgaa tgtttttcca agtagataca
     7441 ccgcaatgat attcactact acaagcaagg catttggtaa agcattaata agtgctgtag
     7501 gcaaataacg gtaagtgatt tttcttttat cttgttcaaa agataaaaag aaggaaggat
     7561 acccttcaat cgctaaatca atcagcgtga tttggatagg gataaacgga aatccaattg
     7621 ctgtaacggc acatataatc gataaaataa atgaataaat cgttttgata aaaaagatgc
     7681 ctgagacttt cgtcacattg ttgaccactc ggcgtccttc aaacaggact tctggcaatg
     7741 tagtaaaatc agagtccaac aataccagat tcgcaatctg acgtgttgct ccatcacctt
     7801 ctgccatagc aatgctgcaa tctgcttctc ttaacgccaa aacatcattc acaccgtcgc
     7861 ctgtcattgc aaccgttctg ccgctttctt tcaattcatt taccagcaat ttcttttgct
     7921 gcggactcac tcgtccaaaa accgtatagc gatgagcagc ttctcgcact tccatttctt
     7981 cagtaatttg agaaagatcg acatacgcat cataattcgg taatcctgca cgtctagcga
     8041 tattggagac agtaaaagga ttgtctccag aaatcacttt cagatccaca ccttcctcat
     8101 gaagataagc tagtgtttct tgagcgtttt ttcggattgg atcatcgatt tcaaacagag
     8161 caaccggctc aacaaatgct ggtttttctt cgttcaattg ctgatttgga gcgatcccca
     8221 acataagtac gcgatagcca ttcgcttggg ctgtttttag actgtccgga agccgatctt
     8281 cttccaccaa acgttcaggc gctcctacgt acacgactcc cagttctgga aattccatcg
     8341 cgccccattt gcgatcagaa gaaaatgcga ctttgtttgt cacatcatac cgattagaaa
     8401 gctcaaaata atctctcata gcccgcatag tgatgttgtt atcactgctt tctgccagat
     8461 acgtccccat aatttctggg atggctttct cgtacagcgg atgtagtgct tctacccgct
     8521 ggatactcat tttcccttca gtaatggtcc ctgttttatc caagcataat gtatcgacat
     8581 gggccagcgt ttcaattgaa tacatgtctt gaacaaggat tcgttttttt gccagtttgg
     8641 ttacccctgt cgttaatgca atacttatta gcaataccaa accctttgga agcatgccta
     8701 acaaagctgc tgcagatgca ataacagaaa ttttgataga agcatcgcgg atgaacagtg
     8761 cttccaaaaa tagtatgatc cccaaaggaa taatcacgaa actagtgaat ttggatactt
     8821 tgcgaatcga atctacaagt tcagaacgaa tagctttgtg agtctttgcc tcgctagtca
     8881 agcggacagc ataattatct gcacctactc ggataacttc agccatgaca gaaccgcttg
     8941 tcaaaaagct tccagaaagc aattcagctc cagtttcttt gacaatcaaa tcagattcgc
     9001 ctgtcaaaag agcttcattt gcttctgccc gtccagatat aacctgtaaa tcggctggta
     9061 tctgttctcc tgctccgatt tttaccaaat ctcctaatac cagctcttta gtagaaatcg
     9121 attgttcttt accgtcccgg atcactagta cttctgattg ggaaacaatc gataatttcc
     9181 gtacaagatt tcttgcatgt atttcttgat aaattccaat aacgatattg acgacaataa
     9241 ttgctaaata cgccatgttg gaatacgcac ctacaaataa cagacaaatc ccaataccaa
     9301 agttcagaaa attgaataac gtcaatatat tatcgctgaa tatttgtttt gtactttttg
     9361 atgcatcttc ctggtagtca ttttgctggc cagaagccat tttctgaact acctcttgct
     9421 gtgtaagtcc attcattttc tttttctcca ttttccgcca actttcaaaa ataatcacgt
     9481 gtttaagtct agcgtgttcc aagattaaaa acaacttgtt tttataaaat ttatagaaaa
     9541 acacacgaat gattttacaa aaaagatgcc aacctttttg tttgaatcac ccgtgtgctg
     9601 tttttattat caatctatct ttacttatat tgttcctcca aacggctcat ccaccaacct
     9661 agtgctgtac cggctataaa aagaacagga caagctagta ccccagcaac tcccaatccc
     9721 gcgtaatcgg taggaatgat cataatcgtt gaagcaaaaa ctacacctaa aatgaaatgg
     9781 aatagcttgc tatatgcctt tgagaaaatg taatccatga tttttgacaa tccaagaaca
     9841 caaagaatcc caccaattcc gatcggcaca atcacttcaa gtcgtacttc tttaaatccg
     9901 tcagccatcg ctttatacaa tcccatatac accaaaaaat tagaagggct caatcctggg
     9961 acgatcatac ccaaaccaat caatccgccg gcaatcagcc atgttccaaa attctgatcg
    10021 acttgcccgt caaataattg agcgccgtat aataaaaaca aataagccag aacaaaagta
    10081 acaaccatga tgatcacatc agaagtatct cttccttttt tccctgcttc tttccataac
    10141 gcaggaacag ttccagcgat acatccgaca aaaaaccata atatcgttgt ttcatacgca
    10201 cctaataaaa aactgactaa aaaagaaagt agaaagatgc ctgtcactcc gcctagacca
    10261 acaggcagaa aaaacaaaac attttctttg aaattttttg taaggtgggc taaaaaagaa
    10321 atcagccgct catacatgcc aaagacagct gctaatgcac caccgctgac tcctggcaag
    10381 ataaatcctg aaccgataaa cattcccttc acaaaccgta acatccagtc tttggttgtg
    10441 taccccactt cttgattttc cattttttgc cttttttcca ctatttgcgt actccttctt
    10501 tcatttaatt ttattcatga ttgaacattt cctgctattt tacttaaaga ctttttcata
    10561 ggtctctatt attttttcat tcattttgac aatatcagga taaaacatgt tacctccatt
    10621 atgatagaga tagccgccat tgtaaagcaa ggactggatc cgccaatatc ggtattgttt
    10681 cccttcctca tttcctaaaa gtgggctcag cacatcttta gaatacgctt ctgctaattc
    10741 tactgtgttt tctccgccat gattttttac ataggcaata taatctttcc caaaattata
    10801 tgcttggaca gctgtccata aatcgcatcc ttgtttcttt gcttctacta tcatttccgc
    10861 caaatatttg acaccttgat caatactgtc agctggatta tcaatgatct cagcttctcc
    10921 atgcagactt tccgagctct gcatgagatc tgtccctttt ccttttgatt cggtcaatat
    10981 tatcgccttg atcaaattct cgtagttttc catatgataa ttttttgcag ctgttgttat
    11041 ttctatttga tagctttcca ctcgttttac cgaacgataa gttcttcctc ataaaaacaa
    11101 caccgcacca ataaccgtca gaatgatcat gtaaaaaaga atacggataa tagataatcg
    11161 ttttcgcttc atttcatcac ctagttattt tgacatcttt ttttatctta ctatttcttt
    11221 atcttcttgg ctatttcttc cttgatttct gtgtacgcct taagaaaaaa tgaattttcc
    11281 tgtatttttt gacatcttac gaaaatgatc gttgtttttc agtaaaaaca attgcccaat
    11341 gactgatata atcgttatac tttacacagt aggtgattat gtgaaaaaag aaaagaaata
    11401 tgcaaaaatg ccagatggga gcgaaattta ctatgaaaag agcggtcaag gcttccctct
    11461 ctttttactg catggcaatg acggcagcgg ccgctttttt tcagaacagg tccctgtact
    11521 cgaacgctac tataccgtgt atctagtaga tagccgagga catggtcgtt cgacaaacga
    11581 agcatctatg ttgaattttc agttaatggc cgaggatctg aatacgatca tgttgctgga
    11641 aaaaatcgat caggcagatt ttcttggatt tagtgatgga gcaaatctag cactggtatt
    11701 tgccagcagc ttccctaaaa aagttcatcg attgatttta aattctggaa acacgcttgt
    11761 taaaggcgtt cgtttttctg ccagagtcat cagtaatatt cattatgcat gggtatggtt
    11821 actctcttta tttcgcccaa gtcttcgcaa aaatctttta gttatcaaac ttttgctgca
    11881 tgacatcggg cttacagaaa atgatttaaa aaaaatcaac tcccccactt tgatcatcgt
    11941 gggaaaaaaa gatgtaatca agctaaaaca ttctttgtat atagctaaaa ctataccaaa
    12001 agcttccttt gtgcttgtga aagaacaagg acatgaatta gctagaaaag atcctgaacg
    12061 cttcaatcga gaagttcttc aatttctttc tgaaacataa ctattaggaa tagaaggtga
    12121 tttatttgtt aaaaaatacc atacaatggc tgaaaaacca cctaggactg ttcaaaacaa
    12181 tctttttgat ttctgtcatt gtcattattg tcggtgaatt gatgtccatc ggtaaaactc
    12241 tatctatcca gcaattagga gaaacgtttg ccactatacc tgtatggaaa agcggactaa
    12301 tgctattgat tggtcttatc tctgtgttac ctatggttgg ttatgatatt attttgaatc
    12361 ggatgcttga acaaaagcag aatcctcgtt acctgtttga gacaagctgg ctgatcaata
    12421 cgatcaacaa tattgccggt ttcggcgggt ttgttagtgt ggggcttcgc tctgaactat
    12481 atggacaaaa gaaagagagc agaaacgtga tccaagcatt atcgaaagtt ttcttgtttt
    12541 tgatggctgg actttctatt tacagtctgc tttctttttt acttgtagtc ttcacgccag
    12601 ttgctccttt tttgaagcaa tattggatat ggttgatcgg cggcggtcta tattttcctg
    12661 tcgtcttttt atttactact ttcaaaaaga aaggattcct tggtggtacc tcacttaaaa
    12721 ctcaaggaca gctattgcta gtttcattgt tggaatggac cggtgtcctg tttagtttcc
    12781 tttctgttgg ttatttaatg gaaatccgga ttgatctgtg gcaaacaatt ccattattca
    12841 ttgctgcttc agtgatagga atcgtgtcta tgattccagg agaaattggg agtttcgatg
    12901 taatgatgat tatcgggttg tccgctattg gtgtgccaag agaaatagtc gttgtgtgga
    12961 tcttgctcta tcgcctgttc tactacatca ttccgttttt gattggaatc gtctttttct
    13021 tcaaaaatat cggttccact tttgatcagc gttattctgg tatccctaaa caattagcaa
    13081 cagaaattgc gcataaaatc gtggttgtct tattatactt ttctggaatc atgctagtac
    13141 tttctgccac tatcccgcaa gcttttacag agtttcgctg gcttcatagt ttgaacccat
    13201 tgaaatttca ttttattatc caatttccta gtatactatt gggatttctt ttaatcatta
    13261 tgggcagagg gatcgcagca cgtgtgaaac gagcatattt gcctacaatc ttcttgattg
    13321 ttctagcttt actttatgtt ttattaagtg attttagttt cacaccggtg gttttcttgt
    13381 ctattctgct tctgatcatt cttgcttcta aaaacgaatt atttcgggaa caattgatct
    13441 attcatggga gtggcgtacg atcgatggaa tcattattgg tgcactgtcc ttgctttaca
    13501 tcgttattgg ggtatacaat ctgccggatt tccctcatcg gcgtcaccat tttatttcct
    13561 ttttcttatt tccttctgaa aagatttggt tttcgggatt gttggccatc atcgctgtta
    13621 gtttcatgat cgttctattt gttcatttcc ttcaaggaga aaagaaacag attggcgaag
    13681 cctttaatga agaaaaggcc ctgaagatcc ttacaactta tggaggaaac tccgatagtc
    13741 aattgatctt cttaaaagat aagcggatgt ttgcctatga aaaagacggt gaaccaactg
    13801 ttcttctcca attcgcttgc ttcaataata aatgtattgt aatgggtgat ccttctggta
    13861 agaaagaaga ttttccagaa gcaatcgaag cttttatcga agaaacagat cgactatgct
    13921 atttaccagt tttctacgaa acaagcgaag agattgttat gattttgcat gaattcggct
    13981 acgatttcat taagatgggg gaagaagctt atgtagacct gaatagtttt acaacttcag
    14041 ggaaaaaaat gaagggcact cgtgctgtct tgaatcggat cgaacgagaa ggatttacat
    14101 ttgatgtctt acaaccacca ttttctactg agcaaatgag tactttcaaa aatatctctg
    14161 ataattggtt gggttcacga aaggaaaaag ggttttcttt aggctttttc tctgaagatt
    14221 atctacaaag agcgcctatt gcagttgtca aaaataccca agaagaaatt gtcgcttttg
    14281 caacgatcat gcctacttac acgaacaata aagtaggaac gattgatctg atgcgatatg
    14341 attctaaaaa agcaccttct ggcagtatgg atttcttgtt cttgcatttg tttgcctata
    14401 tgaaagaaga acatatccaa tggttcaatt tagggatggc ccctctttca aatgtcggaa
    14461 cttccagaaa gagtttttta caggaaagga ttgcatactt ggtttacgag tttggttctc
    14521 atttttattc tttccatgga ttaagagaat ataaaagcaa gtacgctaca aactgggtct
    14581 cacgttatac tctatattct agagatagtt ggattgcata tgttatgatt gctttgctga
    14641 ttattgataa tgcgccagtt gagcagacac aaggtagaat aagtggtttg aaaaaatgga
    14701 tacgaagaaa gtattaaaaa ctgcataccg atagatgaac actctcatga tgaataaaag
    14761 agactgaagt aaaacgaaag ttcatgcttc tacaatctct tcttatacaa ttgaagaa
//
LOCUS       DBNICI010000063        14475 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.063, whole genome shotgun sequence.
ACCESSION   DBNICI010000063 DBNICI010000000
VERSION     DBNICI010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.063"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            175..660
                     /locus_tag="AC1ZCC_001533"
     CDS             175..660
                     /locus_tag="AC1ZCC_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HIQ1629792.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            653..1336
                     /locus_tag="AC1ZCC_001534"
     CDS             653..1336
                     /locus_tag="AC1ZCC_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HIQ1629793.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            1462..4107
                     /gene="polA"
                     /locus_tag="AC1ZCC_001535"
     CDS             1462..4107
                     /gene="polA"
                     /locus_tag="AC1ZCC_001535"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIQ1629794.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            4152..4988
                     /gene="mutM"
                     /locus_tag="AC1ZCC_001536"
     CDS             4152..4988
                     /gene="mutM"
                     /locus_tag="AC1ZCC_001536"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HIQ1629795.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            4952..5581
                     /gene="coaE"
                     /locus_tag="AC1ZCC_001537"
     CDS             4952..5581
                     /gene="coaE"
                     /locus_tag="AC1ZCC_001537"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIQ1629796.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            5633..5965
                     /locus_tag="AC1ZCC_001538"
     CDS             5633..5965
                     /locus_tag="AC1ZCC_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HIQ1629797.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            6277..6780
                     /gene="nrdR"
                     /locus_tag="AC1ZCC_001539"
     CDS             6277..6780
                     /gene="nrdR"
                     /locus_tag="AC1ZCC_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIQ1629798.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENNKENN"
     gene            6803..8176
                     /locus_tag="AC1ZCC_001540"
     CDS             6803..8176
                     /locus_tag="AC1ZCC_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HIQ1629799.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            8176..9102
                     /gene="dnaI"
                     /locus_tag="AC1ZCC_001541"
     CDS             8176..9102
                     /gene="dnaI"
                     /locus_tag="AC1ZCC_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HIQ1629800.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            9231..9671
                     /locus_tag="AC1ZCC_001542"
     CDS             9231..9671
                     /locus_tag="AC1ZCC_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HIQ1629801.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            9815..10516
                     /locus_tag="AC1ZCC_001543"
     CDS             9815..10516
                     /locus_tag="AC1ZCC_001543"
                     /EC_number="2.1.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0160105 - tRNA
                     (adenine(22)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase"
                     /protein_id="HIQ1629802.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            10513..11634
                     /locus_tag="AC1ZCC_001544"
     CDS             10513..11634
                     /locus_tag="AC1ZCC_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HIQ1629803.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            11649..12881
                     /gene="pepT"
                     /locus_tag="AC1ZCC_001545"
     CDS             11649..12881
                     /gene="pepT"
                     /locus_tag="AC1ZCC_001545"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HIQ1629804.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            13013..13921
                     /locus_tag="AC1ZCC_001546"
     CDS             13013..13921
                     /locus_tag="AC1ZCC_001546"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIQ1629805.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            13956..14228
                     /locus_tag="AC1ZCC_001547"
     CDS             13956..14228
                     /locus_tag="AC1ZCC_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HIQ1629806.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            14239..>14475
                     /locus_tag="AC1ZCC_001548"
     CDS             14239..>14475
                     /locus_tag="AC1ZCC_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIQ1629807.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTK"
BASE COUNT         4953 a         2528 c         3185 g         3809 t
ORIGIN      
        1 caaaaacatg agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt
       61 ttcacgacct ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta
      121 cgctactttt ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac
      181 ataggtaagc cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta
      241 tccttaacag aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat
      301 gcaaatcctt tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc
      361 gttccatcag tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga
      421 ccaggatcac atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa
      481 acactgactt ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt
      541 tctgtagaag cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa
      601 ccgccacaag ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa
      661 tccaagtaca tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg
      721 tatgggactt gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca
      781 attagcaact tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact
      841 gattttagta atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg
      901 atttattgcc tactctcttt tgaatggcat aacattagcg gttactcttg catactacga
      961 tattggaaca gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt
     1021 tgtcggagta acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt
     1081 aatcggagtg atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga
     1141 gctgttcatc tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca
     1201 aagcatccgt aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt
     1261 catggcattg aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt
     1321 tggacggaac aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg
     1381 tatggtcccg ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact
     1441 ttacagtaag gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt
     1501 gtggcttttc gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac
     1561 ggtcttcata caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa
     1621 gagcaaccga ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa
     1681 atgtatgcgg agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg
     1741 ccatatatac gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat
     1801 gaagcagatg atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc
     1861 gtcgtgcttt caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat
     1921 atcactgtaa aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa
     1981 tatgatgggt tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct
     2041 gacaatatcc ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa
     2101 tatggctcag tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa
     2161 gaaaatctga taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat
     2221 acttcagcgc ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat
     2281 aaactcgttc cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc
     2341 gtagaagaag aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag
     2401 gaaatgttta ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca
     2461 gaggaaatcg tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta
     2521 tctgtattcg aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa
     2581 gtatatgatg cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga
     2641 atcgattttg atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat
     2701 atcgaaggaa tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac
     2761 ggcaaagggg cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg
     2821 cgaaaagtag ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa
     2881 aaccaagaag atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg
     2941 gaaataagcg ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct
     3001 gctcgcttaa gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg
     3061 aattcaccaa agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa
     3121 aaaacaaaaa ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca
     3181 ccaatcgtag aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc
     3241 gagggactgc tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca
     3301 ttgacccaaa caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga
     3361 ttagatgaag ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt
     3421 tattcttctg actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa
     3481 catttgaaag ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta
     3541 tttggaatcg aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg
     3601 aattttggta tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc
     3661 cgaaaagcag cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa
     3721 tatatggaaa gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac
     3781 cgccgtcgat atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa
     3841 cgaacagcta tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg
     3901 atcgaattgg ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt
     3961 catgatgaac ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg
     4021 aaggaagtta tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg
     4081 ggaaaaacgt ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt
     4141 gaaaagaaaa aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt
     4201 tagtagtagg aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac
     4261 cagagacacc catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta
     4321 gagggaagtt tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg
     4381 aaggaaaata tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt
     4441 tcttttttga ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga
     4501 caatcgtcga aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc
     4561 ctttgccaga tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag
     4621 caatcaagcc attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag
     4681 atgaagcatt atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca
     4741 aagaggtaga acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg
     4801 gaggtaccac gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag
     4861 cactgcatgt ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga
     4921 aaacaaaagt ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg
     4981 caaagtagga tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc
     5041 caaagtcttt cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac
     5101 tgttgagcct ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt
     5161 gcagcctgat gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga
     5221 aaaacggcaa aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca
     5281 aatcgcctct ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga
     5341 aggtcattat gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca
     5401 gcttcagcgt ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag
     5461 tagtcaactt ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg
     5521 aacaaaagaa aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg
     5581 agtatctgtg tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc
     5641 attaaaaaat caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct
     5701 caataaagaa aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga
     5761 aaagcagtac caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa
     5821 gcggcaagat ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct
     5881 cccagaaagt gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta
     5941 tacaggaatc gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat
     6001 gagaaggtgt tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta
     6061 agttatttct cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg
     6121 ggacacaatt acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg
     6181 acagtcgcgt ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat
     6241 cctttatata aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat
     6301 aataattcac gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt
     6361 cgcgaatgtg agaattgttc gttccgattc actacatttg aacgaattga agctgctccg
     6421 ttattagtaa tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga
     6481 ggactcatcc gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc
     6541 aaccatgtcg aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt
     6601 ggtgaatatg tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca
     6661 agtgtttatc gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg
     6721 gataaagcaa aagaaaataa taaagagaat aataaagaaa ataataaaga aaacaattaa
     6781 gaaacaaaaa ggaggcattt ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat
     6841 ttaccaagga agaaaagcgg ctccgctgag agaagacagc aagaacgcct tgctgtatct
     6901 ttatcagcca ctaatcggcg gagaagcctt gagtatctat ttcaccctgt tatcagaaat
     6961 ctctcaagaa tctggcaaag gtcctgaagg gatgcatgca gatctgctta gtagtctagg
     7021 ctgcggtatc ccgcaatttt atgatgcaag aaaaaagcta gaaggaatcg gcttactgga
     7081 tgtttatttt aaagaagata gtgatctggg acctcgtttt atttatgagc tgttagaacc
     7141 actcattcct aaagcatttt tcaaagactc tgttctggct tttcttcttc tggaaaaagt
     7201 tggtgaacga cgattccaga agcttaccca attctttgaa cctaaaaggc atacagcaga
     7261 gggatatcaa aaaatcacaa aaaaattttt agatgtttat cgcttcaatg aagcagtgta
     7321 cgctgctaat caagaaaaca tcgaatcgct gaataaatct tttgaagaat ctgaatcagt
     7381 agcattactt gaagcagatt cgacattcga ttggcagttt ttgaatgatt ggctggcaaa
     7441 aaaacatatt gcaagtcttg atgatccaat catcaaacaa gtcgacatgt atcatcagct
     7501 ttacggtttc gatgaattga cacttggcga actgatcagt caatcttatg actttagtga
     7561 acaaaaagta tctgaaaaag aactgcaaaa agccattttg gcaatgcaga gacgtccggt
     7621 gaagcaaaca aaagaaacag ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga
     7681 acatttgaca aacgcagcgc ttcagctgat aaaagaagca cagagcattc caccgatgaa
     7741 atatctggaa gcaatcaagt cagaaaaagg cggatatgtt tctaaacaag aaaactggtt
     7801 actgcaagat cttgtttctc aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa
     7861 ttatgtacta gtgatcaaga ataatgctag cttaaatgcc agttatgtaa atacagtggc
     7921 aaatgaatgg gcacaaaaga aaatcgccac agcagaagaa gcgatccagc acattcggca
     7981 aataagtaat caagcaaaac aatcaaaaca aaagaagcaa acaaattatc aaactggcaa
     8041 gcgaaatgtt cgacgggaaa agcttcctga ttgggtcaac cagccaaaag atgaaaaaca
     8101 gatcagcagc gagaaaaaag cagagattga ccgccgtttt aaagaatacc tggcaaaaaa
     8161 ggagggtgat atctagtgga agacgtaggc aaagaattga agaggatcat cagcaaacgc
     8221 aattatcaag aaaagtatgc tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa
     8281 gcttttctaa gcgcacacca agaagaattg acacaagcgg atattgaaaa aagttacgct
     8341 aaattgtatg aattcgtgca agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc
     8401 gctcccggct atgagccgaa gctgacgttg aattttcatt atgtggatgt cacttatgta
     8461 ccaacggaag cattgttagc acggcaaaga caagaagaaa tcagaagccg agtaaaagca
     8521 atggatatgc caaaagatat tcaatcggct tcttttgaaa actttgaacg tacagatgga
     8581 cgaggatatg cgagcttaga agccctagat tttgtagaga aatatactgc tgatccaaaa
     8641 gggtatcata aaggactgta tctagtagga agttttggta ttggaaaaac atatttatta
     8701 ggagcaacag cgagagaatt agcgattgct ggattcacca cgacgctagt ccattttccg
     8761 acatttgccg ttgagatgaa acaggcaatc ggaaaagatc aagtggcaga aaagttagat
     8821 gcagtgaaac gttcacctat tttgatgatc gatgacattg gtgcagatgc gatgagtagt
     8881 tggatacgag acgaagtatt cggtgttatc cttcaatatc ggatgcagga acagttacca
     8941 accttcttct catcgaattt tacaatggat gaattggaac agcatttgtc cgtcactcaa
     9001 cgtggagatg aagagccgct aaaagcgaaa cggataatgg aacgcatacg ttatttgaca
     9061 aaagaaatcg aaatgactgg tcgcaaccga cgcaatggtt gaacagttga caagtccaac
     9121 tgacatttga cctagtagta aaagcaggtc agcgaatcat tatatggaca gaaatgaccg
     9181 gagacaatca ccgcaacaag taatgcagaa acttaaacgg aggatttttt atgagggcta
     9241 ttttttctag tatatctgta gttggtctat tttttctttt agcaggctgc ggtagcgcag
     9301 atagcaacac aggttctgtt gattcgattg atgcgggtca aacagtaaaa acaagcagta
     9361 cgaaaacgga gagacaaagc atatttgtcg gaacgattga aagtatcact caagtagaag
     9421 cagaagaaga aacgcttcag ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga
     9481 atatgggaac gtcattcagc aatgacggag tgatcttaaa tgctactgct gaaaaaataa
     9541 gcgtggacat aaatgaattg aaaatcggtg acaaagtcca atttacatta gttgagatgc
     9601 cgatcatgac gatgtctatc ccaccgcaag ttcctggaga ttcgattatt gatgtttcat
     9661 tgaacaaata accaataaca ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa
     9721 aggtatattt tttaaggaga tatgggaatt tttttattcc tagtctcctt attttatttg
     9781 gaaaatgcta taattatcta gaaggagtga agctatgaac catttagatt tatctcagcg
     9841 cttagccgca gtgggtgact ttgtccctga atccgctcgt ttagcagaca ttggatcaga
     9901 tcacgcttac ttacccgttg cattgatatt aagaggaaaa atcgaatatg cagttgctgg
     9961 tgaagtcgtt caaggtcctt atgaatcagc acgtaagcaa gtcaaaaaag atggattaga
    10021 agaccgtatc accgtacgcc ttgcaaatgg tctagatgct atccagccac aagatggtat
    10081 tacagctgtc acgattgcgg gtatgggagg ttcactgatc cgagatatcc tagaagctgg
    10141 caagttgaat caacgcttaa gcggacaaga acgtttgatc cttcagccaa acatcggaga
    10201 gcgtacatta cgatcttggc tgcaagaaaa tcagtataaa atccaatcag aaaaaatact
    10261 tgaagaaaat aaaaaaatat atgaaatcat tgtagccgaa aaagaacaaa aaccagttac
    10321 ttacaatgaa atggagctga tgttcgggcc atttctgctt gaagagaaaa gtgagatctt
    10381 caaatcaaaa tggacaagag agcttcaaca aagaaaagct atccgtgaac agctcaaaca
    10441 agcagctaat caagaggcac gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga
    10501 ggttttaaag ccatgacaat cagcggacgc actttcatcc aaaagtttga aagctattgt
    10561 cctcagtggc tagctgaaga aggagaccca gtcggactgc atatcggaac attggataag
    10621 ccaatccaac gagtaatgat gacattagac gttcgtccag aagtggtcga agaagcaatc
    10681 aagaaaaaaa tcgatttgct gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg
    10741 ctggttactg atcagccaca agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc
    10801 gtctatgcag cacacacgaa catggatatt atttgggatg gactgaatga ctggttttgt
    10861 gaacttctcg gtattgaagt ggagagttat ttagtaaaga ctcatgagat tcattataaa
    10921 aaactggccg tttatgtacc tgtggatcat gcacagaaaa tgcgtgaagt attagctgca
    10981 gctggtgctg gtacacaagg tgattatacc ggtacaagtt ttacctcgat tggacacggt
    11041 cgatttactc cggaagctgg tgcacaacca gcgattggga aagtagggaa gacagaacaa
    11101 gttcaagagg caaaagttga ggttattttg ccggaaacta tcgaaaaaca agtgattcaa
    11161 gccatgcggt cagctcatcc ttatgaggaa cctgcttatg atcttttcgc tatagatgaa
    11221 ccagtcgaaa tgtttgggtt aggacgagta ggtgaattgc cgcaagaaat ttctatagag
    11281 gcatttgtag aacaagtaaa agaagcgttc caactagatg gactgagaat agtccagcca
    11341 aaaaatgcaa aatcctctgt gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt
    11401 tatccgcaag caatcgccca aagagcagat gtctatatca cgggagatat ctattatcat
    11461 acggctcatg atatgcaaag cgctgggttg attgcaattg atcccggtca ttatattgaa
    11521 tcgttatgca agcaacgatt tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg
    11581 gatattaact ttttcgtttc tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag
    11641 agaatactat gtacgaaaat cttttgccac gttttttacg atatgtcaaa acagaaacac
    11701 gctccgatgc aacaagcaaa acgactccat cgactcagac acaagttgct tttgcgcaag
    11761 aattgaaaaa agaattagaa gagttaggct tagctgatgt tttctataat gaagataacg
    11821 gattcgttat gggaacatta ccaagcaatg ttgatcatga agtgcgttcc attggattta
    11881 tttcccacat ggatacagca gattttaatg cagtaggagt caatccacaa atcattgaaa
    11941 actatgatgg cgagtctacg atcgtactag atgcggaaga acgctataca ttgaatacaa
    12001 aagattttcc aaatttgaaa aattatgaag gccacacatt gatcacaaca gatggcagta
    12061 cattactggg agcagatgac aaatcaggga ttgcggaaat catgactgca atggagatct
    12121 tgatcaataa tccttctatc cctcacggta ccatcaaagt ggcttttggt cctgatgaag
    12181 aaatcggtgt cggagcggat aaattcgatg ttgagcaatt cgatgtagat tttgcttata
    12241 cagtcgatgg tggtccttta ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa
    12301 atattacgat ccaagggaaa aatgtacatc cagggacggc aaaagataca atgatcaatg
    12361 ctctacagtt agcaatcgat tttcataatc agcttccagc cgatgaagtt ccagaaaaaa
    12421 cggaaggata cgaaggtttc ttccatttga tggcattgga tgggaatcct gaagaagctt
    12481 ctatgagtta tatcattcgc gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa
    12541 tcacacatat ccaagaagag ttgaatcagc gatttgatca agaacgaatc aaagtagata
    12601 tgtatgatca atattacaac atgaaagaaa tcattgaaaa agatatgagc atcgtggaat
    12661 tagcgaaaca agcaatgatc gagcttggta tctctccagt gatcgaacca gttcgtggag
    12721 gaacagatgg ttcaaaaatc tcttacatgg gtattccgac gccaaatcta tttgccggag
    12781 gagaaaatat gcacggacga ttcgaatttg tttctttaca agtaatggaa aaagcaacag
    12841 acgtgattgt aaaaattgca gaattgaaca caaagagtta aaatcgaaaa agaacagctt
    12901 atgacaaggc gttcgtccca atgatggaat gtcatctgtc taaggctaaa gggcaggaca
    12961 atggaattgg ttgtcccgcc ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa
    13021 gctgctagtt ttttataaca aaagttcagg gaaagatgag ggtgagaaat tagcacgctg
    13081 gtttcaagat tatgtaaaag aaaagcagcc agaattatat gtagaactag ctgaaactgg
    13141 cccatcgatt gatgatgcgg aattagtcaa gaaagcagaa gccatagaag ctgatacttt
    13201 agtaattata ggtggagacg gcacgattca tcatatcgtc caagcgttcc aaaaaacatt
    13261 atctcaatac catgtcggat tacttccagg tggaactgtc aacaaccttg cacgtgttct
    13321 tgagattccc ctcaaaaaag aagaagctgc tgacgtgatc ctggaaggca atgtacggaa
    13381 aatagactat ggtcaagtca atgatgaagt cattatctct acactgacaa tcggaatctt
    13441 agcagataca gcagtttgga tcagtcaaga agaaaaacag cgttacggct cgtggatctt
    13501 tatcaagaag ttttttaagt tattgctgaa gaagaaaaaa tatcatttgg atttagaaac
    13561 agacgaggag cattggaaag gaaaatccca actgttgaca gtcacgatgt ctaactccgt
    13621 gggcggtttt acaaattttg acgactcagc gaccctagat gatggaaaat ttcatgtaac
    13681 gatcgtacct tccttagata ttttccgatt tattttttat ttgcctagaa tcatgagagg
    13741 caaattctat acgatccccg gcattactta tttcacagcc aaacgcatga aaatccaagc
    13801 gcaggaaaaa tctgtgggca ctcgaacgga tggagacacg acagatgatc ttccagttga
    13861 attatctgtt gtatcaaaag gattaacagt ttattcacca aagaaagaag aaaaagctta
    13921 gaaatagtag ttaacagaaa gttggaagaa gagtaatgga tgtattgtta cacaatcctt
    13981 tgtggtttta ccttttcatt gtgaattttt acttattttg tctgatgggg tatgataaac
    14041 atcaggctag aactagcggc tggcgtgttc ctgagaataa tttgatattt gtcagcctgt
    14101 taggcggtgg tttgggagga ttgatctctc gaaaagtttt ccatcataaa actcggaaaa
    14161 agaagtttac tataggattc gctgcaggtg tacttgttga tttgattctc atttattttt
    14221 tccattaagg agcaggagat gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg
    14281 cttttgctga ttgtgatttt tgccattatt tattatttaa tcaatcaatc atttgcagat
    14341 atctttgccg aattgctgtc taccagtgtt gttgttttgg cagcggctgt tatattaggg
    14401 acagtttacc aaatcgctga agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg
    14461 aattttacaa cgaaa
//
LOCUS       DBNICI010000064        14445 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.064, whole genome shotgun sequence.
ACCESSION   DBNICI010000064 DBNICI010000000
VERSION     DBNICI010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.064"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(50..1468)
                     /gene="nhaC"
                     /locus_tag="AC1ZCC_001549"
     CDS             complement(50..1468)
                     /gene="nhaC"
                     /locus_tag="AC1ZCC_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HIQ1629808.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(1871..3094)
                     /locus_tag="AC1ZCC_001550"
     CDS             complement(1871..3094)
                     /locus_tag="AC1ZCC_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIQ1629809.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVNNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(3125..3964)
                     /locus_tag="AC1ZCC_001551"
     CDS             complement(3125..3964)
                     /locus_tag="AC1ZCC_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIQ1629810.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(3981..4661)
                     /locus_tag="AC1ZCC_001552"
     CDS             complement(3981..4661)
                     /locus_tag="AC1ZCC_001552"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HIQ1629811.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(4663..5736)
                     /locus_tag="AC1ZCC_001553"
     CDS             complement(4663..5736)
                     /locus_tag="AC1ZCC_001553"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HIQ1629812.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(5792..6030)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(6073..6306)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6383..7054)
                     /locus_tag="AC1ZCC_001554"
     CDS             complement(6383..7054)
                     /locus_tag="AC1ZCC_001554"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIQ1629813.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(7141..9789)
                     /gene="mgtA"
                     /locus_tag="AC1ZCC_001555"
     CDS             complement(7141..9789)
                     /gene="mgtA"
                     /locus_tag="AC1ZCC_001555"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HIQ1629814.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(10131..10305)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            10478..10915
                     /gene="ndk"
                     /locus_tag="AC1ZCC_001556"
     CDS             10478..10915
                     /gene="ndk"
                     /locus_tag="AC1ZCC_001556"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HIQ1629815.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(10937..11803)
                     /locus_tag="AC1ZCC_001557"
     CDS             complement(10937..11803)
                     /locus_tag="AC1ZCC_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIQ1629816.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(11963..14056)
                     /locus_tag="AC1ZCC_001558"
     CDS             complement(11963..14056)
                     /locus_tag="AC1ZCC_001558"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIQ1629817.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSETNRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            14235..>14445
                     /locus_tag="AC1ZCC_001559"
     CDS             14235..>14445
                     /locus_tag="AC1ZCC_001559"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629818.1"
                     /translation="MVTASLHGIQKPVSRYSYKKRGCDKILVAAPYFLMQDSFFTTSS
                     LNGVLLPTPRPQVTIFIKHEKLFFEI"
BASE COUNT         4194 a         3090 c         2455 g         4706 t
ORIGIN      
        1 cgtaaaaggg attgtgacaa aagtcttgtc acaatccctt tttatgtttt tattgttcta
       61 attgcgctgt cttgcttact gcttcttgcg gtttcacaaa tttttttgag attccccaac
      121 ctgtgaaggc actgaccatc gaaaacacag gagaaagcaa actgaaaaat acgaatggca
      181 agtaagtgag tgtaggaata ccaaatgtac ttgctacgaa acttccggca attccccaag
      241 gcactaagta gttgattact gttccaccat cttctagcgt tctacctaat acagttggat
      301 caaatcctgc tttttcataa gtttctttaa atgcattacc aggcagaata acagatagga
      361 attgttcacc aataaaaata tttactccga tacttgtcat caatgttgta aaaatcaaat
      421 ttggtgtgtt cgtcatcttt cttgatactc gttccatcac ggttgagatc aagccaaatt
      481 cgatcaacaa tccacctaat gacaaagtta gaataatcaa tgacacagta ggcatcatac
      541 tttcgattcc tccgcggcta agcagtgaat cgatttgttg gtttccagta tttgaaacaa
      601 accctgattc gatgacccca gccaacttcg ttaaggaagt ttgtgggttt tgaataaaaa
      661 tcattcctac tgctacagcg atattcaaca aaatcgttaa aattgccggc acctttttcc
      721 atgcacacag taacattaaa aataaaggaa tcagtgacca gattgaaata gtaaagtttg
      781 cttctagaac atcagtcact tcacgaatag atgaaagact tgcggcttta cttgtgtgtc
      841 ctaaaatcat gaataaaaag aaagaaacaa caaatgccgg gacagttgac cacatcatat
      901 gcttgatgtg tttgaacaaa tcggcatcaa caatagctgc tgctagattt gttgattcgg
      961 ataatggaga catcttatct ccaaaaacgg cacctgaaac aatagctcca acaaccagcg
     1021 ctggatttaa gcctagtgtt gtcccaatac cgaaaaatgc aatcccaatc gttgacataa
     1081 cagtaaaggc actccctaca gcgctaccta cgattgcaca cacaacaaat acagaaggga
     1141 caaaccactg gacactgatc aatttgaacc caatgaccat tagagttgga atgatccctg
     1201 cttgtatcca tacagcaatc aatgaaccga ctaaaataaa aataaagatt gggatgattc
     1261 ctggtttgat gccattgatg attccttcgt gcagccaatc tgtcgggtat ctttttatca
     1321 ctgcatacaa catgaccatt ccgatcgctg ctaagatcgt gatattaggt gagaccttta
     1381 atccgacaac gccaatcgca atgcctaaaa caataaccat taatacaagt gttgcttcta
     1441 aaaaacttgg tgttcctttt tttcccatga taatctctcc ttttttagtt gttcttatgt
     1501 tctaaaaaag ttaagatatc ggattactcc gccgcaattc atccgcgatt cctcctttat
     1561 aaaaataaaa agacctgttt taaactaagg acgaatgact ccgcggtacc accttaatta
     1621 aaaacagatc tgtttttcac tttcaaactt ttattcaaat gtatggactt tccatgtaat
     1681 tcgtcaatta ggcttgatcg gttcacagcg accaccgact ttctgaacaa aacacctaac
     1741 cgctactgcg tgaaaagttg acttgtacga agtttacctt ccataagatc atttgtcaat
     1801 gaaaaagaaa atattttttc atttttctga aaattatttt tataactttc acacacaagt
     1861 ttatttttta ctagaaagta gcgccctctt gtttttttct tgctactatt tcttgatgtt
     1921 cttgcttgat tctttctaat agctttggct ctttcaacag atcaagagct gtattggcga
     1981 gtagttctgc tgcaatcgca atagaagcaa ggcctttgtc acttttagca gcagctttga
     2041 attcttctgt gtgtccggca atatatgtct cagagattgc taccgtaggc tggattgttg
     2101 gtatgacttg gctgacattt cctacatcag aagaaccgag acttgttctt tgttccgttt
     2161 ctatttcttc atctggaaca cctgcttctt ttgcatgctg gaagaataag tcatcaaaag
     2221 aaggagtaac gatgacatca tcaactgcat tttggaacaa tccaaactca aaagtcgtgc
     2281 ccgtacttat cgcagcacct cgtacgatat tttccacttt ttcatatact ttatctaatg
     2341 tttgccgatt tcctgcacgt aaatagaaac gtccagcagc gtaatctgga atgacgttgg
     2401 cagctactcc tccgtctgta atgatcccat ggatattgac atcttttggc aattgcaagc
     2461 gtagagcgtt gattccatta aacacttgga tcaatgcttc caaagcattt actccttttt
     2521 caggtgctgc tgctgcatgg gaagcgacac cgtaaaattt gatgtcgact ggatcattcg
     2581 ctaaagattc tgtcgttttc ccataacgaa aagctggatg gacgcataac gctacatcga
     2641 catcctcaaa aaaaccttct cgaacaaagc tgcctttagc tgatccgttc tcgccccctt
     2701 cttcccctgg ggtgccatat acacggattt ctccaccagt ttcatcaatg aattgcttca
     2761 ctatgctggc ggctaatacg gagtaagtac caaacaaatt atgcccgcat gcatgaccaa
     2821 tacctggtaa agcatcgaat tctgccaaaa aggcaattgt gggaccgggt ttattggaaa
     2881 tataacgtgc atcaaaggct gttcggtgtc cagctacttc tttttctacc caaaaccttt
     2941 ctttttctaa ttgtttaatc aacgctgctg aggaataaaa ctcatagtta tttacttcag
     3001 gatgttcgtg gatatctaga gcaagtgctt gatattctgg taaacgatat tggattagat
     3061 ccttcaattg gtttgttgag attgttgttg tcataaaaaa ttcctcctat gctagtcatt
     3121 tttcttattt tctcccgaat gtttcccatg caggcacgct tgatcctttt gatgttttct
     3181 cgattacttt gactgtgtct tctgtttgat aagcgtctac tattttttga tacgttttat
     3241 tgttttcatc ttcttttctt gcaacgatga tattcacata aggacgtgca tggtcatcca
     3301 ctggttctaa gaagatggca tcagaagcag gattaaatcc agcatctaca gctacaccgc
     3361 tattgatcac tgaaatgtct acatctgaca aagcacgtgc tgtttgggaa gcatctaatt
     3421 cagaaatgtc tagatttttt ctattctcgg taatatcaga aacagtgggc gcttgtcctt
     3481 ttgccttatc cactttgatc aatccagcag tttgcaataa cagaagagct ctgccgccat
     3541 ttgtaacatc attagggatt gctacttttg ctccgtcttt gatatcagct acatccttca
     3601 aagtgttaga ataaattccc agcggtgcat tgacagtatt tccgatcgat actaaatcag
     3661 ttccgtgttc tttattgtaa ttgtctaaaa agaattgatg ttggaaagaa ttcaaatcaa
     3721 tcgatccatc tgctaaagcc acatttggtt gattgtaatc tgtaaatttc acgtattcca
     3781 aattaatgtt ctcctttttc aaacggtctt tcacgttgtc ccatacatct gtatcttctc
     3841 caataatccc tagttttacg atagtttctt ttttgtcagt tgtatctgct tttccttggc
     3901 tacaccctgc caaccaaata attgctacaa atgtaatgac tacaaaacga ataatttttt
     3961 tcattttttc tcctccactt tcaatgtgta atttttttaa tcagttgatt actgataaat
     4021 tgacttaaaa aaactaagat tagaatcaat aatgtagcca caatcgttac atctgtttgg
     4081 aatcgattat accctctcga tatagcaaga ttccctaatc cgcctgcacc tactgctcca
     4141 gccatggtag tcagtccgat cacgttgatg atagtcaacg ccgagacccg aatgatacca
     4201 ggcaaacctt ctaacaaata cacacgtaaa atgataccga ttggactggt tcccatcgct
     4261 tctgccgctt cgatcacccc aggatcaact tctagcagtg cgttctcgat ttgacgagca
     4321 aaaaacggaa caactccaaa aatcaatggc actaaagctg ctttcgcacc aatcgtcgtt
     4381 ccaactaaga aacgagtagt caatgctaaa agagaaagca agataatgaa agggatggaa
     4441 cgaatgatat tgatcagctt atcaagtatt tcaaatagca gacggttagc tagtaatcca
     4501 ttagggcgtg tcactactaa cgtcacacct aagatgattc caatcatccc gccaatgaat
     4561 gctgtccaaa aagacatata tagtgtttgc ttcgtcgctt cgacaaattc atccggaatc
     4621 aaataaacat tgggcaaata tgttttaaag aaatcagtca ttttatcttc ctccttcgat
     4681 cactttgata cgactatcta aatcgttttt ttgtaaaata tttattttta caccagattt
     4741 gatcaaataa tccaatgctt ttttccgctg tttggtttct ccagctaaag taacgatcaa
     4801 actccctaac ggcgtttctt gtaataactc tacattgcca tataaaatat tcgctgttac
     4861 ttgatatttg ctgtacagat gagcgattag aggttcgttc gtctgatttc caatataact
     4921 taattccacc agccatttat tggcgatctg atcggcaaaa gcagaatggg caatgatcgt
     4981 ttctaatgct tggtcaagat gagtagctgt tcgaataaaa tctttggtca attcttcttc
     5041 aggattgctg aagatctgga tgctgctacc tttttcaacg attcttccat cttccataac
     5101 cgctaccttg ttgcagattt ctttgacaac ttgcatctca tgagtgatca ggacgattgt
     5161 taaacctaac tggcgattca atttttttaa caatgcaagg atctgaaggg tcgttttcgg
     5221 atcaagtgca ctggttgctt catcacataa taatacctta ggttcattcg ccaaagctcg
     5281 tgcgattgct actcgttgtt tttgtccccc cgacagttgt ttaggataag cagatgcttt
     5341 ttcttccaaa ccgacaagtt ccagtagttc attcactttt tgacgacgtt cctgtttcga
     5401 ttttccagaa tactttaaag aaaaatcaac gttgtcaaaa atagttcggc tttccattag
     5461 attgaaatgc tgaaagatca taccgatggc ctttctttct tttcgcaatt ctttgggaga
     5521 gagctgactc agattcgtct gattgacaat gacttctcct gctgttggtt tctgcagcaa
     5581 attcatcaca cgaactaacg tacttttccc tgcacctgag taaccgacga ttccataaac
     5641 atcgcctttt tcaatcgtta aatctacaga atctactgcc tgtatctctt tttctttttg
     5701 tttgaaacat acagaaacat ctcgcaattc aatcattaaa attcctcctt atctaaaaaa
     5761 agcgtcctca agaaaatgaa aatgtcattt tcttgaggac gcttttttgc gtgttaccac
     5821 ctcatgttca agctgtgttc acacacaact cctcttcaag tacgatcata ctctagcaag
     5881 atatcggtcg cagccgtcat cgccttacat ctatgctcgg gatgaattgc tcgatggcca
     5941 ttttccaata gtcgttcctt gcttcttctc agctaccgaa gctctctgtc aaaggttctt
     6001 ctatcgtact taccatttca atgcaaataa tattcaaaaa aaaagtcctt ttactaacat
     6061 aaactatgtc agtaaaagga cgacgtatcg cggtaccacc ttttttcgga agtttccttc
     6121 cctcaagccc aacatctcaa aaagagagat tggcgttcgt tttaacggtc gaacagttcc
     6181 gaatttgcct acttatcgac aaattattct ccaaggccat tttcagcttt tcttacttga
     6241 ctcttctcac cagccagagc ctctctgcaa agtttccaaa cttactttcc ttttcaacga
     6301 aatcgatatt ttgttgttgg gattaatcta catgaggaaa actgaaaagt caagtacgaa
     6361 attcaaatta ttttttctat atttattttt ctgttttttt atttaacatt tcaataacag
     6421 ttcgaagatc ttttccttcc gcaagaagtt tcaatttttc cttttcttcg acggtataat
     6481 ttcggacaaa actggaatca ggtatcaaaa caacgcctaa tcctgctcgt gaagcagctg
     6541 ttgctcctac taaagaatct tccactgcaa aggctgaact agcttctaat cctgttcgtt
     6601 ccaaagctgt cagataagga atcggtgaag gcttgttctc cgacacttcg tctccgaatg
     6661 taaacgtgtc caagtaatca gctaatttaa agtgatccaa gatatctgtt gctcttttcg
     6721 taacagtggt cgtagcaatg cctagtttca tatgacgttc ttttaagtct gccaatattt
     6781 cttttgcata tggttttaag tggatggctc cgcttgtcag ctgttgataa aaatattctt
     6841 cacgcttcgc tcggacttcc tgtacttttt cagggctacc tgctgctttc actaaaatat
     6901 caaaactttg tttccagttt tttccgcccc aagcgtcaac gatttctttt gggatatgga
     6961 tatcaaattg ctgaaatccc caaagccagc catcacgata cgttttttct gtgtcaacta
     7021 acaaaccatc taaatcaaat attgcacctt tcatctatac attaatcctt tccaaaacaa
     7081 tacacatagt ttagcacgac tggtccaaaa agaacagaag caaagaacca gccttctcgt
     7141 ttataagagt tcttggaatc gacggatata gattttcttc acagctgtca caagcgccag
     7201 ataagcgatg actgtagcaa ataaccataa ccagtaattg ccaggaagtg gcatcaagcc
     7261 taattcagca ccaaaacttg taaaaggaag gacagaaccg acaataattc ctaatgtagt
     7321 gataacaaac atggcaaatg ttgcgttgct ttggataaat ggtactttcg gtgtacgcag
     7381 tgcatggagc actaaggttt gtgtccatag tgattcgaca aaccatcctg catggaataa
     7441 tgcgatgaaa atcactcgct gtgaactatc caatgtatgg aaacttccgc ccactacagc
     7501 aggacaaata acaaaataca tcaacaagta agtagtaata tcaaatatcg aacttgttgg
     7561 accaaagtaa accatgaatt ccccaatact agatgcattc catttttttg gctcgtccaa
     7621 atattcttta tccatccgat cccatggcaa ggaaatgcag gaaacatcat agatcagatt
     7681 caaaaacaaa agttgtaaag gcagcattgg taaaaatggc aggaacgtac ttgcaatcag
     7741 cacggaaaac atattgccaa aattagaact tgctgtagcc ttgatatatt tcatgatatt
     7801 cccaaaagtt tctcgtccag aaagaaggcc tctttccaaa atcgtcaaat ctttttctaa
     7861 aaggatcacg tctgcagatt ctttcgcaat atccacagct gtatctacag aaataccgat
     7921 atcagccact ttcattgcag gagcgtcatt gataccatct cctaaaaatc cgacagtatg
     7981 accattttta cgtaaagctg tcgtcaaacg tgctttttgc tgaggattca attttacgaa
     8041 tacttcatgg tcttcagcta cttgtgctaa ttcatgatca ctcatttccg agattttctc
     8101 accggtaacc aattcattga tctctagacc tacttgacgg cacactgaac gtgtaactaa
     8161 ttcgttgtcc ccagtcagaa cttttacagc tacaccatgt tctttcagag ctttcaacgc
     8221 ttgtttcgtt gtttcttttg gtggatcaag gaaagcaaga taaccaatca aaaccatgtc
     8281 tgactcatct ttgactgaaa attctccaac gacgcttgga ttggtctttt gtgcgacacc
     8341 aatcactcgt aagccatctt cattaagtga ctgtactttt gcaagtacgg cttcttttcg
     8401 ttcttttgtt aatggtgtaa cagtaccgcc aagatcaata aatttagaaa tgttcagcat
     8461 ttcttcaatc gcgccttttg tgatcatttg tgttttacca gctggatcag caaccacaac
     8521 actcatacga cgacgttcaa aatcaaaagg aatttcatcc actttttgat aattgatctt
     8581 atctgtatct aaagtttctg ttgcttcatc aatgatagcc acatctaaca aatttttcag
     8641 ccctgtttga tagtagctat ttagatacgc atggcgcaaa acacgatcat cttcatttcc
     8701 ttcacaatcc atatgatatt caagaatgat tttatcttgt gtcaatgtcc ccgttttatc
     8761 cgtacataac acatcgattg caccaaagtt ctggatggca ttcaaatttt tgatcactgt
     8821 tccttttttg gccatcacac ttgctccttt gaccaaattc gtcgtaacga tcattggaag
     8881 catttcaggt gttagaccaa ctgctacaga tagtccaaaa agaaaagctt ctagccaatc
     8941 cccttttgta agcccattga tgacgataac tgctggagcc atcaatgaca taaatttgat
     9001 cagtagcatg gatgttttat ggatgcctaa ttcaaagctt gattgaattg gtttttcaga
     9061 tagcgtttct gctacatgac caaatagtgt ttcatttcca acagaaacaa tgattccttc
     9121 cgcacttcca ctgatgacat tacttcccat aaagacaaga ttttcgtaac ttgtttcgct
     9181 ttgctgatcg tcttcacata ctttcgcatg cttttctact ggatagcttt cccctgtcaa
     9241 tgctgcttgg gaaatgaata aatcctttgc ggacaataaa cgcatatccg ctggtatcat
     9301 atcccctgct gaaagccgaa caagatctcc acagacgatt tcttccatag gaatctctgt
     9361 gtactttcca ttacgtttta cagctgccgt tactttcaca agtgatttca gtttttcagc
     9421 cgcttgattg gaacgtacgg actgaatcaa cgtcattgtc ccgctgacga taaccatagt
     9481 gaagataatg gctacaccga aaagatcttt atctccagga gctgctaata catattccgt
     9541 gatgaatgaa ataattccta atgcgatcaa cactaatgta aatggcgtga tgtatgcttt
     9601 caatatttct acaatgaatg gtgttttttt gccatatgaa atcgcatttt caccgtaatt
     9661 ttctcttgaa cgttcgattt gttcttctgt caatccattt gctgatgcat ggaatagttc
     9721 aaataattct tgtttcgttt ttccagcaaa agctgcatag attttttgtg tatctttctt
     9781 tttttccatc tcaatctctc ctttgtgtcc aaaagacagc tgttctcgct gcttttggtc
     9841 tttagctatt catcggatcg atagagaaaa aactgaaagg ggctgagaac agatagataa
     9901 tagccctggc ctttcctcta gatccttgtt tttcgaatga ctgtcatcca tttgttctca
     9961 tctcctttac taagtaattt taattcatga cttccaatca tttttcgaat agttattttt
    10021 ccaattagac cacctccttt ttatttttca ccgatgggga tttgcggcaa caaaaaaacc
    10081 tctcgccaaa tactcagcga aaggtttgaa aaagcatatt aaaaaaagct tctcagatct
    10141 catcgttgag ttttgacact gtacaacgta aaaagcagat acctgctttt agctacctta
    10201 tgaaaagcct tatctcgaca attcctgttc tacccattgg cgtctctcga cgtttttggg
    10261 cagtagccta tgtttgtata ggagcctcac ctaacaaatc ttattcggtt ttccttcgtt
    10321 aatataacaa ctaaaaaaga ggatgtcaaa tcaattttcc atgtttttca tacttttttt
    10381 aaaatacaca cgttatattc ctgtctcacg cggttttatt tgtctcattt tccacctccc
    10441 gctataatag aaatatagta ggaaacgagg tgctgttatg gaaagaacac ttgttattat
    10501 caagccagac ggcgtgagac gccatttagt cggaagcatc atccaacgtt ttgaagcaaa
    10561 gggactggcc attgctgaaa tgaagtttga aactatgact cctgaattag caaaagaaca
    10621 ttatcagcac ttgactgagc gcagtttttt tgatgaactg atcgattaca tgacttccgg
    10681 tcctgtcgtt tatcttgtgc tggtcggcga ggaagtgatc gatatcgtcc gtaagatggt
    10741 tggagcaaca aaagctgccg atgctgttcc aggtacgatc cgaggagact acgcgctgcc
    10801 ggggactgaa aacatcattc acgcttctga ttcccgtgat gctgcggtaa aagagatcgc
    10861 ccgctttttc ccgccattcg atataaacaa accgtctgaa aaagcggcga agtgatacaa
    10921 attctaaact gctggtctag ttgctgggtt cagcaatggc cagcagtttt ttctctaagg
    10981 acctccgttg attctttcct aacatatatt cgagcactgt gaagaattct tggtagtctt
    11041 tcgtcttttc ttcccctaaa gcagcagaaa tcaatctttg ataataatct gccatcaatg
    11101 tttcatagat cactgtactt ctttctttat gtgataagct ccgtttcata tcttcattca
    11161 cactacgtgc ctttttcaac cgaccatttt ctatataaaa cgaagtcagg ttcattaaaa
    11221 aagtaaagag atactgctca tgattgttca actgtgctaa tcgtcgcatt ctttttttga
    11281 ttttcaaata attgttctca atgaaccgat cactgaatat ccaaggaaaa tcagctagaa
    11341 tagatagttc aaattgacca tagtctggta tctgcatcaa gtattcttca atgattcggc
    11401 attcataacg taaaaattca aattctgtct cagatacttc tgttttttcg accatgtgaa
    11461 gattcaaaac caaataatac agatcttctg ttttcttaaa atgcctacgg ttattgctga
    11521 tctctttata aggatcaaag ctgtcttctt gtacccgatc tagcattcgt ctttttttct
    11581 ttaactttat tggttcatcc agctggtttc gcatccattc atattctgca agtgtcatgt
    11641 tcattcgctc taaaaatgat ctcaatgtta caaatgacat ttttttcact gaatttgtct
    11701 caatcccgac tagagaagtc ttaaaacaga ttccttccgc taattgggac tgtgtcaact
    11761 tcttgttgac tcgaagtttc ttgatcaatt caccatcata cataatagtt ctccttttta
    11821 atttaaattt ttccacattc aataaataga tacgttaaaa aggaaaaata cacgaaaaaa
    11881 ataaaaaaat gtttttttca taaaaaatac caaccttcaa gcattagaat gttgtctaac
    11941 tactgaaggt tggtttgatt gtttattgat tatttttttg ctgttcaacg tctgttcttg
    12001 ttactggata taagttggtt gttgttttat taccattttt tgaaaagaca cttgtcgaat
    12061 gttcgccttt ttgcttttcg attgcttcat tacgttccgc ttcattttta tagtcatatt
    12121 ttttcggatc aatcgtctca aatccgtttg gcacataaaa ccgcaagaga ttttcctgat
    12181 tcagcttatc tgaaagactt aacgcagtct tgactttttc ctgatctttg gagatttcct
    12241 catcgattcc tgctgctttc gtatcgatga tttctcctgt ctgattattg taagcttttc
    12301 ctcctaggac ggtgtatttg ggtgtcacaa agttcccatt tcgaaaagca actacttgat
    12361 catgctgaga agataataag tctgtaccaa aatgaagata atctttatca tcgattccca
    12421 atagatgtag taatgtcggc aaaacatcga tttctccacc gtattcatga ttgactgtcc
    12481 ctttagtata accaggcata tggatcatca atggcactcg ttgcatctgt gcgttgtcaa
    12541 attcatccca tgtatctgga tctttaccaa gagcgcttgc taaatcttta ttatctgtat
    12601 tcgagatccc aaaatgatcc ccgtaaatca caaagattga attttggtaa ataccagatt
    12661 ttttcaaata cgtaaaaaat tgttccaatg attgatccag ataatgtgcc gttcgaacat
    12721 aattatctac tgtgctattg ccagtattca aagatggaaa gtcgaaattc ttgtcatccg
    12781 tataataagg cgtgtggttg gttacggaaa gaaatttcgt gtaaaacggc tgttgtagat
    12841 gctcaagata ttgggcagat tctctgaata aatctttatc taatatccca taacctaacg
    12901 tttcgtcgga ttcatcaaaa taagatcgat caaagaaatt gtcataacct aagtttttat
    12961 atacatgatc tctattccaa aaactagcta cattcccatg gaaaacagcg cttgtatagc
    13021 cttgcttttg tccaaggatc gctggtgcgg cttgaaagac attgtctgac ccaagttctg
    13081 tgaataatga gccttgaggt aaaccaaatg tgccagtttc caacatattt tcagcatcac
    13141 ttgtctttcc ctgtcctact tcatgaaaga aattgtcaaa gctgatcgtt gcttgattct
    13201 ggaaaatact atttaaaaac ggtgtgactt cttgtccgtc taccttcata ttaatcaaaa
    13261 actgctggaa actttctaaa tggagaacga tcacattttt ccctttagcg atcccaaatg
    13321 tttcgggatt tggctctgca taatgtttct ttgtataatc taaaacttca tcaatcccat
    13381 tactgcttgc atgcgcgcga acactgctag tcattcctgt tttgattccg tcataaatag
    13441 tatacgcatc gattcctaga tatttgacaa tatatgaacg gtcaaatgtt cgctgcagca
    13501 attgtggacg gttggtttcg ctcagcgcta gatttcctga aaaaataagc cctgaaagac
    13561 aggtaacagc gatagccatc ggtttgccga cagggttgct tttgataggt attttcttga
    13621 ccttcaacca aaccagtagc ccaatgaaca cagcgatatc cagccagtag atgacatcat
    13681 ggggcttcat caaagaaaag gagcttcctg acaatccttg cgacactttc gaaaagccta
    13741 atacagattt gactgtaatg aaatctgtga attcccgata aaaaataata ttcaagtaca
    13801 acaaaagcgt atttaaaata tcaatgatca aaagaatagc taacgctggc tttggctttt
    13861 taatatacaa agaaaggcca aaaaacaata aggtagtcgc aattgggttg atccacaaga
    13921 taaagtactg gatcgtacct tccacaccta atgaaaaatc tgcatagtag gcaatgatcg
    13981 tcttcagcca gaaaaagaaa acagctgtca aaacgattgc taaacgattt tggcacaatt
    14041 ttttaagttg tgtcacgtat atcacttctc tctattcaca ttttttctta ttttgctcct
    14101 gaaaaatgaa aatttcctaa aaaagttgtt atcttaagat ttttttattc tttctaaaga
    14161 ttattccgtc cacttttcac agcataaaac cgctgcgatg taagcgatta aactaatgaa
    14221 gagataaaag tcttgtggtg accgcttcac tccacggtat tcaaaagcca gtttctcggt
    14281 attcgtacaa aaaaagaggc tgtgacaaaa ttcttgtcgc agctccttat ttcttgatgc
    14341 aggacagctt tttcacaact tcttccttaa acggtgttct cctgccaact cctcggccac
    14401 aagtcacaat atttataaaa catgagaagc tgttttttga aattg
//
LOCUS       DBNICI010000065        14281 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.065, whole genome shotgun sequence.
ACCESSION   DBNICI010000065 DBNICI010000000
VERSION     DBNICI010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.065"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(26..601)
                     /locus_tag="AC1ZCC_001560"
     CDS             complement(26..601)
                     /locus_tag="AC1ZCC_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIQ1629819.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(606..1580)
                     /locus_tag="AC1ZCC_001561"
     CDS             complement(606..1580)
                     /locus_tag="AC1ZCC_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIQ1629820.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1604..1648)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1863..2831
                     /locus_tag="AC1ZCC_001562"
     CDS             1863..2831
                     /locus_tag="AC1ZCC_001562"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIQ1629821.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2944..3221
                     /locus_tag="AC1ZCC_001563"
                     /pseudo
     CDS             2944..3221
                     /locus_tag="AC1ZCC_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            3339..3575
                     /gene="secG"
                     /locus_tag="AC1ZCC_001564"
     CDS             3339..3575
                     /gene="secG"
                     /locus_tag="AC1ZCC_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIQ1629822.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3678..4433
                     /locus_tag="AC1ZCC_001565"
     CDS             3678..4433
                     /locus_tag="AC1ZCC_001565"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIQ1629823.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4436..6793
                     /gene="rnr"
                     /locus_tag="AC1ZCC_001566"
     CDS             4436..6793
                     /gene="rnr"
                     /locus_tag="AC1ZCC_001566"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HIQ1629824.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6815..7279
                     /gene="smpB"
                     /locus_tag="AC1ZCC_001567"
     CDS             6815..7279
                     /gene="smpB"
                     /locus_tag="AC1ZCC_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIQ1629825.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7581..8300
                     /gene="atpB"
                     /locus_tag="AC1ZCC_001568"
     CDS             7581..8300
                     /gene="atpB"
                     /locus_tag="AC1ZCC_001568"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIQ1629826.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8353..8568
                     /gene="atpE"
                     /locus_tag="AC1ZCC_001569"
     CDS             8353..8568
                     /gene="atpE"
                     /locus_tag="AC1ZCC_001569"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HIQ1629827.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8659..9183
                     /gene="atpF"
                     /locus_tag="AC1ZCC_001570"
     CDS             8659..9183
                     /gene="atpF"
                     /locus_tag="AC1ZCC_001570"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIQ1629828.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9170..9712
                     /locus_tag="AC1ZCC_001571"
     CDS             9170..9712
                     /locus_tag="AC1ZCC_001571"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HIQ1629829.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9735..11291
                     /gene="atpA"
                     /locus_tag="AC1ZCC_001572"
     CDS             9735..11291
                     /gene="atpA"
                     /locus_tag="AC1ZCC_001572"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIQ1629830.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11305..12207
                     /locus_tag="AC1ZCC_001573"
     CDS             11305..12207
                     /locus_tag="AC1ZCC_001573"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HIQ1629831.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12230..13636
                     /gene="atpD"
                     /locus_tag="AC1ZCC_001574"
     CDS             12230..13636
                     /gene="atpD"
                     /locus_tag="AC1ZCC_001574"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIQ1629832.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13650..14072
                     /locus_tag="AC1ZCC_001575"
     CDS             13650..14072
                     /locus_tag="AC1ZCC_001575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HIQ1629833.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4729 a         2578 c         3080 g         3894 t
ORIGIN      
        1 attttgctaa tccagctctg tttattcagc ctatttttct ttttgaatat atcaagcgtt
       61 gtggcaacat agtgatgact aatgaaacaa cgacgataga caacaaacga tcaaggtagt
      121 ctgtacctgc ctgtaccaag atcacacttg ttgtctgact taaacctaac ccatgtaata
      181 gctgaacgat catactggat cctgaggagg tgatcccgcc aaataactga acggtaatga
      241 tcgaagaaac gatcgttcca ggtaccgaca aaatcaaagc gagtaataac agtttcaagc
      301 tgtttttatc tttgccgaga tatcttggca aaatcaatcc agcaagcaag cctgtgagaa
      361 tttggattgg cgaataatac agagaaaaaa tatctgtagt cactccactt aaaataccgc
      421 tggacaatcc tgtcagcatt ccaggtacag gcccaagcaa tgcaccagca aaaatcgttc
      481 cgatagaatc gagatagatt ggcaaacgaa gccataaagc aatcgttcct ccaatataat
      541 tgatcccaat acacaaagcc atcactgtaa gtacgcgagt tgccattttg tttttattca
      601 ttttgttagc ccatctttaa cctttccaaa tctttcataa tcgtttcttc ttgtgcattt
      661 aaaagaacgg ttaaaaactt tcggaaaaaa agacgagtat ctacttttgt cataatttta
      721 ctgttagcag gtttctgcca aaattcataa cgatcaacca atgtttgtcc acgacttatt
      781 ccttgagttt ccacagcagt gaagcttttg aaaccttcac agatggcctc ctcgataaaa
      841 taagcaacag ctaacggatc attgatgaca catcctaaaa ttctttcttg tttccagtgg
      901 aagtcaaaat aaaaacgagt gatcgctttt agatactctc cctcttcagg attcatttga
      961 cagcagtatt cgaggatagt cggtgtcaag acgatttctc ttgtcacatc taacccaacc
     1021 atttctatcg tttgtttgag attctcgaat acataagaag ctgcttcagg atcacaccag
     1081 taattgtatt cagcaactgg cgaacagtta ccatggcttt tgtaagttcc acccatcgag
     1141 acaaaacgtt ccatatgttt cccaatattt gggttgactt tcaaagcaga agcgatattg
     1201 gtcaaaggac ccaaagcaat gactgatgta tttgtctttt ctttgaaata atcagccaaa
     1261 aaatcaacgg catggagagg ttctgcctgt tttctcaaga tcatcgggaa atttgtttcc
     1321 cctaaaccat ccataccatg agtgtcttgt gcactgatga aaggttgctt caaaggtttt
     1381 tccattcctt gataaacagg aatgtctaaa cgatcacagc gttccaggca tttcagcaca
     1441 ttctgcgtac ctatatctgc agggacattc ccacaaacta cggtaatagc aactacttcg
     1501 atatcagggg atttgacagc taatgataaa gctaaagtgt cgtcgatccc tggatcgcaa
     1561 tcaataatta cttttcgcat tatttccaat ctcctttctt tagagatact tagtttttta
     1621 ttctgggaag tttccgaacc agtccaagac gagcgtcttg attataatgc acgaaattag
     1681 tatactggct tttattgcga atgtaaatgc tctcttacaa gtaaatctat tgaggagaac
     1741 tttaagtaaa tgatacaatg aaaaagaaat ggaatttacg tgtgccgaaa ataaagaatc
     1801 ctatggatcg gtacattgga taatagatag attatagttt ggcagaaagc ggcgggtgta
     1861 caatggttat tttttatttc attcttattc tgataattat agggatcagt taccattttc
     1921 tttcttattt ttatgcaaag aaattagtaa gagcgggagt acagagagga tatgactggt
     1981 atgaagacac tggtcatcgt ttgatggacc ctgctgcagt cacgtcagta gaaaaaaaac
     2041 gacaaaaatt agaagaagag tgtgaaagct tttgggagca gggaacaccc aaaacaatca
     2101 aaagttatga cgggttgaaa ctagctggtc agatgttcat ccatccaaat aagcagaata
     2161 aatgggtcat ttgcgtacat gattaccgaa gcacagggaa aagagacatg tctcatatag
     2221 gtaaaagata tgcagagaaa ggattcaacg ttcttgtacc tgatttacgt gcgcacggag
     2281 aaagtgaagg agaaatcatc ggaatgggat ggctagatcg gctagattta attgcctgga
     2341 tccaactcat cttagatgaa cagccgaatg cgtccattat tttacacggc ggatcaatgg
     2401 gagcttctac gatcatgatg gcaagtggtg aaaaacttcc atcagcggtc aaaggattta
     2461 ttttagacag cggatatgtt tctgtttatg cagaattccg atatatgtta agtaaaatca
     2521 ctgtctttcc taaaaaaatg gtcatgagat atgcgaatca ttatgcccag aaatacgccg
     2581 gttactcctt aaagcaagca tcagctacga gacagcttgg cagcaatcat ctgcctttat
     2641 tagtcatcca tggtgaaaga gatcattttg tccctacaga agcagcgtac acgattcaaa
     2701 atgctacggc aggggataaa gcactgctgc ttgttccgga agcagaacac ttggaagcat
     2761 cgatgaaaga tccgaaaacc tattggacag tgattttttc atttattgaa caaagaataa
     2821 cgatgaatta attttctctt cttttttgta agtaattcga ggataaataa ttgtttatag
     2881 atcaggagac tgctaaaata aggtaggaat aaaaaaatat cagttatttg aggagtgcgg
     2941 catatggaaa tcaaagaaga aaaaatcgtc ttgctttatt caatgatgaa caacaagaga
     3001 tcggagaaat gacatggtca gatgcgggcc cagacatcat gatcgttgac catacatttg
     3061 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
     3121 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
     3181 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata aggataacag ctgaaagttt
     3241 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     3301 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     3361 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     3421 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     3481 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     3541 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     3601 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     3661 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     3721 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     3781 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     3841 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     3901 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     3961 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     4021 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     4081 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     4141 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     4201 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     4261 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     4321 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     4381 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     4441 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     4501 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     4561 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     4621 agtgaaactt cctttaaaac caatcctgat cgaaggaaca tttcgagcaa atgaacgagg
     4681 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     4741 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     4801 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     4861 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     4921 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     4981 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     5041 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     5101 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     5161 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     5221 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     5281 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     5341 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     5401 tgtatatttg actgatcgag taattccgat gatcccgcaa cgtttatcga atggtatctg
     5461 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     5521 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     5581 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     5641 tcagctcgta cctatgttcc aagaaatggg tgaattgcac cgaatccttg aaacgatgcg
     5701 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     5761 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     5821 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     5881 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     5941 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     6001 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     6061 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     6121 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     6181 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     6241 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     6301 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     6361 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     6421 gggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     6481 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacagaaag tgcgagtaaa
     6541 agtagtcaaa gctgatccag atgaacgagc agtagatttt gaacttgttt ccgcagaaga
     6601 ggttacagcg ttggaacggc caaaaacacg aaatggaaaa tcaggaaaac gcgaaggaaa
     6661 acaagcagga aaacgcccag aaagaaatgg caagaagaac aaacgatata gcgatagtaa
     6721 gcaaacgttc ggtaagaaaa aagggaagaa gccattttac aaaggtgtta aaaagaagaa
     6781 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaattaat
     6841 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     6901 ggtcttgcaa ggaaccgaga tcaagtcgat acgaaacagc cggatcaatc taaaagatgg
     6961 atttattcgc gtccgcaacg gagaagcttt cttgcataat gttcatatca gtccttatga
     7021 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaaaaa
     7081 acaaatcatc cggcttgaaa atgaattgaa aaatactgga atcactgttg ttcctttaaa
     7141 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcgaaag ggaaaaaatc
     7201 ttatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     7261 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     7321 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcat aggttttttc
     7381 ttagatcagg agcaccgtgt ataaagaaaa attcgcttcg tttttgactg aaataaaaaa
     7441 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     7501 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     7561 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gttccacatt ggaccaattt
     7621 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     7681 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     7741 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     7801 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacatcggtt
     7861 tagtaacaaa aatcgtttta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     7921 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     7981 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     8041 ttccgatgaa actgattgaa gaatttacaa acttattaac acttgcttta cgtctttatg
     8101 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     8161 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     8221 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     8281 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     8341 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     8401 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     8461 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     8521 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     8581 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     8641 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     8701 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     8761 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     8821 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     8881 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     8941 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     9001 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     9061 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     9121 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     9181 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     9241 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     9301 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     9361 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     9421 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     9481 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     9541 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     9601 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     9661 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     9721 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     9781 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     9841 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     9901 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     9961 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
    10021 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
    10081 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
    10141 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
    10201 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
    10261 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
    10321 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
    10381 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
    10441 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
    10501 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
    10561 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
    10621 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
    10681 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
    10741 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
    10801 cggttgatgc cggactatct gtatctcgtg taggaggttc tgcgcaaatc aaagcaatga
    10861 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
    10921 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
    10981 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
    11041 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
    11101 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
    11161 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
    11221 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
    11281 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
    11341 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
    11401 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
    11461 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
    11521 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
    11581 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
    11641 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
    11701 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
    11761 tctgatcagc caagctttga tgaagtccgc aaaattgtaa atatggcaac aactatgttc
    11821 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
    11881 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
    11941 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
    12001 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
    12061 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
    12121 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
    12181 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    12241 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    12301 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    12361 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    12421 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    12481 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    12541 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    12601 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    12661 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    12721 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    12781 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    12841 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    12901 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    12961 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    13021 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    13081 attgcaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    13141 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    13201 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    13261 ggcttcttct tcaagtgctt tagcacctga aatcgtggga gaagaacatt ataaagtagc
    13321 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    13381 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    13441 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    13501 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    13561 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    13621 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    13681 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    13741 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    13801 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    13861 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    13921 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    13981 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    14041 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    14101 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    14161 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    14221 tggaacaccg tatatcagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca
    14281 a
//
LOCUS       DBNICI010000066        14117 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.066, whole genome shotgun sequence.
ACCESSION   DBNICI010000066 DBNICI010000000
VERSION     DBNICI010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.066"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(29..3682)
                     /gene="rpoC"
                     /locus_tag="AC1ZCC_001576"
     CDS             complement(29..3682)
                     /gene="rpoC"
                     /locus_tag="AC1ZCC_001576"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIQ1629834.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3761..7378)
                     /gene="rpoB"
                     /locus_tag="AC1ZCC_001577"
     CDS             complement(3761..7378)
                     /gene="rpoB"
                     /locus_tag="AC1ZCC_001577"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIQ1629835.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8106..9074
                     /locus_tag="AC1ZCC_001578"
     CDS             8106..9074
                     /locus_tag="AC1ZCC_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIQ1629836.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9092..9997
                     /locus_tag="AC1ZCC_001579"
     CDS             9092..9997
                     /locus_tag="AC1ZCC_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1629837.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            9994..10800
                     /locus_tag="AC1ZCC_001580"
     CDS             9994..10800
                     /locus_tag="AC1ZCC_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1629838.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10957..11925
                     /locus_tag="AC1ZCC_001581"
     CDS             10957..11925
                     /locus_tag="AC1ZCC_001581"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIQ1629839.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12242..12766)
                     /locus_tag="AC1ZCC_001582"
     CDS             complement(12242..12766)
                     /locus_tag="AC1ZCC_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIQ1629840.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13053..>14117
                     /gene="scm"
                     /locus_tag="AC1ZCC_001583"
     CDS             13053..>14117
                     /gene="scm"
                     /locus_tag="AC1ZCC_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HIQ1629841.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         3997 a         3046 c         2784 g         4290 t
ORIGIN      
        1 atttgtcaca acctcttttt taaaatatct attttgtttg attgttcatt gcgtcttctg
       61 cagccatttg cgcttcaata tctgaaatac tgtagacatt ttcgcttgct acaccgactt
      121 cttttggttc catgtcacga taacgttgca taccagtacc agctgggatg atcttaccta
      181 taataacatt ttctttcaaa ccaagaagag gatctttctt accgcggatc gcagcatccg
      241 tcaagacacg agttgtttct tggaatgatg cagcagataa gaaactattt gtttctaatg
      301 atgcttttgt gatacctagt aagacaggtc gagaagttgc aggaacgcct cctgagacta
      361 gcgtattgta gttttgatct ttaaagtctg cgatgtctaa taatgtacct ggtaagatat
      421 ctgtatcacc tggatccatt acacgaactt tacgcagcat ttgacgaacc attacttcga
      481 tatgcttgtc gccgatttct accccttgca tacggtaaac acgttgtact tcacgaagca
      541 agtagttttc aacagataat acatcgcgaa cttgcaacaa ttgttttgga tcgattgatc
      601 cttctgtcaa tggtgcacca cggtggatca tatcgccttc tgctactttc atacgtgctg
      661 tataaggaac agtatacgta cgtgtatctg tttttccttt gattgttact tctttggtac
      721 gtgttgcagg atcttctgag atatcgatga catcccctgt tacttctgtg atcactgctt
      781 gccctttcgg attacgtgct tcaaagattt cttggacacg aggaagacct tgagtgatat
      841 cgtctccggc aaccccacct gtatggaatg tacgcatcgt caactgagta ccaggttcac
      901 cgattgattg agcggcgatc gtaccaactg cttctccgac ttcaacgtca gagccagttg
      961 caaggttgcg tccgtaacaa tgtttgcaga caccatgttt tgtattacat gtgaagacag
     1021 aacggattgt cactgtttcg acacctgcat caacgatttg ttttgcgatg tcttcggaaa
     1081 tcaattggtt tcctgcaatg atcaattcat ttgtttcagg atgacgtact tctttacggg
     1141 tgtaacgtcc cactaaacga tcttctaatg attcgatgat ttcatttccc tcgcggatgg
     1201 cttggatttc aagacctcgg tcagtaccgc aatcttcttc acggatgatt acatcttggg
     1261 ccacgtcaac cagacgacga gtcaagtaac ctgaatcggc agtcttcaag gctgtatcgg
     1321 tcatcccttt acgagccccg tgagttgaga taaacatttc caagacggat agtccttcac
     1381 ggaagttcga aatgattggc aattccatga ttcgaccatt aggagcggcc atcaaaccac
     1441 gcattccggc aagctgtgta aagttggaaa tgttaccacg agctccagaa tcagacatca
     1501 tgaagattgg atttttcgct tccaaacctt ccatcagttt ttgttggatg ctgtcttttg
     1561 cagcattcca aacagcaatg acacgttcat aacgttcgtc atctgtgatc agaccacgac
     1621 ggaattgttt tgtaatagtt tcaacttgtt tatgggcatt ttcaatgatt tcttgttttt
     1681 cattcagtac actgatgtca gcgatcccta ctgtgatacc ggcatgtgtc gaatgtttgt
     1741 accctaagtc tttcatacgg tcaagcatct tagaagtttc ttttactttg aatcgtttga
     1801 agacttctgc gatgatgttt cctaagtttt tcttcttgaa cggtaatact aattcttgct
     1861 ctttgatatg tgcaggaata tcagtaccag cttcaacaaa gtatttgtcc ggtgtttgga
     1921 cggtcaagtt gaagtctgtt ggttcattca gataagggaa ttcaggaggc atgatctcgt
     1981 tgaagatgat cttaccaact gttgtgatca tcgtacgttc tttttgccat tcagtaaatg
     2041 gtttttcacc taatgagtta gggttcacac cgatacgtga atgtaaatga acatatccgt
     2101 tgcgccatgc gataacggct tcgttttgat ctctgaagac cattccttcg ccttcgcgtc
     2161 cttcttcttc cattgtcaag tagtagttac ctagaaccat atcttgagaa ggagtaacaa
     2221 ctggtttacc atctttaggg ttcaagatgt tttgagcagc taacatcaac atacgtgctt
     2281 ctgcttgtgc ttcttcattc aatggaacgt ggacagccat ttggtctcca tcgaaatcgg
     2341 cattataagc ttcacataca agtggatgca aacggattgc gcgtccttgt actaaaactg
     2401 gttcgaatgc ttggatacca agtcggtgaa gcgtaggtgc ccggttcagc aatactgggt
     2461 gttccttgat tacgtcttct aaaacatccc atacttcgtc ttcttgacgt tcgattttac
     2521 gtttcgcatt tttgatgttt gaagctagtt cgcgttgaac aagctcacgc atcacgaatg
     2581 gtttgaataa ttcaatggcc atttcttttg gaagtccaca ttggtacatc ttcaagaaag
     2641 gtccaacaac gataacggaa cgacctgagt agtcgacacg tttacctagc aagttttgac
     2701 ggaaacgacc ttgtttccct ttcaacatat gagaaagaga tttcaatggg cggttacctg
     2761 gtccagtaac tggacgtccg cgacgaccgt tatcgatcaa tgcatctact gcttcttgca
     2821 gcatacgttt ttcattttga acgatgattc caggtgcatt caagtctaac aaacgtttca
     2881 aacggttatt acggttgatt acacgacggt aaagatcatt caagtcactt gttgcaaaac
     2941 gtccaccttc taattgaacc attgggcgta aatctggtgg aataacaggg ataacgtcca
     3001 taaccatcca gcttggttga tttcctgaag cgcggaatgc ttctaaaata tccaaacggc
     3061 ggattgcacg tgtacgtttt tgtccttgtg cagatttcaa ttcttctttc aattcggctg
     3121 cttctgcatc tagatcaaca ttatcaagta attgtttaat cgcttctgca cccatagcag
     3181 cttggaattc ttgtccatat tgttcacgtt tttcgcgata ttcgcgttct gtcaacaatt
     3241 gttttttctc taaattcgta tttcctggtt cgatgactac atatgaagca aaataaatga
     3301 tttcttccaa agcgcgtggg ctcatgtcta atacaagacc catacgtgaa ggaatccctt
     3361 taaagtacca gatatgtgaa acaggagctg ctaattcaat atgtcccatg cgttcacggc
     3421 gaactttcga acgagtaact tccactccgc atcgatcaca aacgattcct ttataacgga
     3481 tacgtttgta ttttccgcag gcacattccc agtcttttgt aggaccaaaa atccgctcac
     3541 agaacaaccc ttcgcgttca ggttttaagg tacgatagtt aatcgtctca ggttttttta
     3601 cttcaccgta agaccagctt ctgatttttt cgggagaagc caaacctatt tgcatacttt
     3661 cgaatttatt tacatcgatc aaaaggggtt ccctccattt catttaatgg caaaccgcaa
     3721 gacggctgat cttcgaccgc cttgcagctc atagaccgga ttagtctaaa ttatcttctg
     3781 cggtttcaaa gttttggatg acatcgtctt tttcatcttc cacttgttca gcagctttct
     3841 tttctaattc tttggcagtt tgctgttcgg caaattttgt caaagcatca acagtgatca
     3901 aatcgtcgtc ttcatcgtcc atgtcgcgaa gttcgatctc tgtttcttcg atatccaaga
     3961 cacgcatgtc cagacctagt gattgcaatt ctttcaccaa tacgcggaat gattccggta
     4021 cacctggttt tggaattggt tcacccttga cgattgcttc gtatgttttc acacgtccga
     4081 ctacgtcatc tgatttgtat gtcaagattt cttgtaaagt ataagccgca ccgtaagctt
     4141 ccagtgccca tacttccatt tcaccaaaac gttgtccacc aaattgagct ttacctccca
     4201 atggttgttg tgtaaccaat gagtaaggtc cgattgaacg tgcatgcaat ttgtcatcga
     4261 ccatgtgagc aagtttgatc atgtacatca caccgacaga cacacgtcca tcaaatggtt
     4321 cacctgtacg tccgtcgtac agaacagttt tcgcatcgct ggccataccg gcttcacgaa
     4381 ctgtttccca tacgtcttca tcacttgcac catcaaatac tggtgttgct acgtggatac
     4441 ctaactgacg tgcagccata cccaagtgta attccaatac ttgcccgatg ttcatacgtg
     4501 aaggcacccc taaaggattc agcatgatat cgattggtgt tccatcaggt aggaatggca
     4561 tatcttcttc cggcataata cgggatacaa cccctttatt tccgtgacga ccggccattt
     4621 tatccccttc atggatttta cgtttttgaa cgatataaac acgaactaac atgtttacgc
     4681 ctggtgacaa ttcatctcca gcttcacgag taaagatttt cacatcgtga acgataccgc
     4741 cgccgccatg tggtacacgt aatgatgtat cgcggacttc acgagctttt tcgccaaaga
     4801 tcgcatgcaa tagacgttct tctgcagata attctgttac cccttttggc gtgactttcc
     4861 ctacaagcaa atcgccatct tggacttctg caccaatacg gatgataccc atttcgtcta
     4921 aatcttttaa tgcgtcttcc ccgacattag ggatttcgcg tgtgatttct tcaggtccta
     4981 attttgtatc gcgggcttct gattcatatt cttcaatatg gatagaagtg tatacgtcat
     5041 cttttaccaa acgacggctc atgatgatcg catcttcata gttgtaccct tcccaagtca
     5101 tgaaaccaac taaaacgttt tggcctagag ccatttcgcc ttgttccata gatggtccgt
     5161 ctgccagagt atcccctttt tcgactttct cacccaaatg aacgattgga cgttggttgt
     5221 agcttgttcc ggagtttgaa cgacggaatt ttgttacatc gtatttatct aatgcaccgt
     5281 tgtcgcggcg tacgcggatt tcagaagcat caacgtattc aacgacacca tcatgtttac
     5341 ataacaaagc agctcctgag tcatgcgctg atttgtattc cataccagta ccaacccatg
     5401 gtgcttgagg attgatcaac ggcactgctt gacgctgcat gttggcaccc atcaaggcac
     5461 ggttggagtc atcgttttcc aagaaaggaa tacatgctgt cgcaacggca actacttgtt
     5521 taggtgatac gtccatgtag tctactttat caatagatac ttctaagttt tcactttgag
     5581 cacgagccat aacaacgtct tgagcaaatg ttccatcttc attcaatggg ctgtttgctt
     5641 gggctacgat gtaatggtct tcgatatcag ctgtcaagta gtcgatttgg tcagtaacac
     5701 gacctgtttc acggtctaca cgacgataag gtgtttcgat aaatccgaat ttatttactt
     5761 tcgcataact ggacaaacta ttgatcaacc cgatgtttgg gccttcagga gtttcgatcg
     5821 gacacatacg gccatagtgg gagtagtgaa cgtcacgaac ttcataaccg gcgcggtcac
     5881 gagtcaatcc gccaggtcct aaggcgaaaa gacgacgttt gtgtgtcaat tcgccaagag
     5941 ggtttgtttg atccatgaac tgtgacaact gagaagaacc aaagaattct ttgatacttg
     6001 ccacaactgg acggatattg atcaattgtt gaggtgtcaa tgtttcagta tcttggatcg
     6061 acattctttc acgaaccaca cgttccatac gtgctagccc gatacggaat tggttttgta
     6121 aaagttcacc gactgaacgg atacgacgat ttcctaagtg gtcgatatca tcgacattgc
     6181 cgataccttc catcaagttc aagaaatagc tcattgaagc aatgatgtcg gctggacgaa
     6241 ccgtttttac agctgcttct ggatagccgt ttccgatcac gtttacttca cgttcagggt
     6301 ctcttggtga aaatactttg atgacttgga cagtcattgg atctgtgacc acaccgtctt
     6361 ctgatggata gtaagtcact gaatttaggc cattatcaat gaaaggagct agtgtttcca
     6421 tgacttggtg agtcagaact gttccttttt caacgatgat ctcgcctgtt tctggatcaa
     6481 ctaatgtttc agctaatgtc agattcaata gacgtgtttt caaatctaat tttttgttta
     6541 ctttgtaacg accaacgttt gctaagtcgt aacgttttgg atcgaagaaa cgagcattca
     6601 gcaagttacg tgagctgtct gctgtttttg gttcgcctgg acgcaaacgt tcgtaaacgt
     6661 ctttcaaacc ttcttctgtt cttgagtcgc ttgcgttttt atgcaagtct ttttcgactg
     6721 tattgcgtaa tgtttcgctg tcgccgaaga tttcaaagat cgtatcatcg gaaccaaatc
     6781 ctaacgcacg aactaatacg gttaaaggaa tcttacgtgt acggtcgata cgtacataag
     6841 agatgtcttt tgcatcagtt tccatttcta gccaagctcc acggttaggg ataacagtag
     6901 aaccgaatcc ttcttttccg tttttgtcga cttttccatg gaaatagaca cctggtgaac
     6961 ggactaactg agaaacgatt actcgctctg ccccgttgat gatgaatgtt ccttgttctg
     7021 tcattaatgg gaaatcaccg aagaatactt cttgtgcttt gatttcgcca gtctcgcggt
     7081 ttgtcaaacg caatgttaca tgtaatggtg cagagtagtt tgcatcatgc gcacgagctt
     7141 ctgctactgt atactttggt tctttcaatt cataatctac aaattccaaa gatagattgc
     7201 cattgaagtc atcgattggc aaaatgtctt caaacatttc tctaagtccc tcatctaaga
     7261 accattggta agagtctgtt tgaatttcga tcaaatttgg taattccaac acttcactga
     7321 ttcttgcgaa acttctacgt tcgcgatgtt ttccgtattt tactacgtgt ccagccaagc
     7381 tcttcacccc tctaaaatgt cattcaaaaa gactacccga tcaattgtta caataatatt
     7441 tcaaatatta tttttaggta acaacagaca ttttttgcgg cttttaggca aaaaaaaacc
     7501 aaaaatagaa cattctcttg aaaccttact tcgtgagctt ctacttttgt ttccttatat
     7561 taaagccgaa acggacaata tttcgtttct gccttttctt catcagatag tttttgcaac
     7621 ttcatatact acacttattt tctataaaag tcaacacttc ctggcgtttc ttctgctttt
     7681 tcgcaacatt ttcttaatgt tttcaatttt atgttttcaa ttatctgaca attcttaatt
     7741 gacagacctt tttatctcgg ttatacttta gaaaattcat tttgtggagg aacttctatg
     7801 cgaaccaaca tcaaccaaat cgcaccaaca caactgaata acttttactt tagctacttt
     7861 agataggttt gtattcatca tgatggatac aaacgttaaa cgacagtttg tatctatgat
     7921 ggctaaagaa ctttgtattg cagcaaaaag ccacgtagac aaaatgctaa gtggctgagc
     7981 agtgaaaata caaagtaaaa gtatcaagca ttctactttt tcaatttatt ttccgacttt
     8041 tcaatccatt tggacatttt gtctgaatgg attttttgtt tcccaaaatt aaggaggaaa
     8101 aagaaatgaa aaaaaatata atcatcggat taggcgctgc cctgctacta gctttaggag
     8161 gttgcgcaca aaaagacaat tccgcagagg catcagatgg acaaaaaaca attggcgtat
     8221 tgcaacttgt acaacataac tcgttagatg ctgcctataa aggattcaaa gaaggattag
     8281 cagaagaagg gtataaagaa ggcgataatc taacaatcga ttatcaaaat gcccaaaaca
     8341 atcaagacaa cctgaaaagt atgagtgaga aactcgtcaa agaagatcct gatttgctac
     8401 ttggaatcgc cactccagca gcacaatctt tattaaatga aacaaccact atcccgatga
     8461 ctgtcacagc ggtcacagac ttggaacaag cccatttagt cacatccaat gaaaaacctg
     8521 gcggcaatgt gactggcacg acagatatgg tgccaatcga aaaacaaatc ggcttactgt
     8581 taaatatcgt gccagacgcc aaaacaatcg gcatcatgta taacaacggt gaagcaaact
     8641 ctaagatcca agctgatttg gcagaaaaag aattgaaaaa agcgggcgtc aaagtcaaag
     8701 tactgacagc aaattccaca aatgatgtcc aacaagtgac gaaaagtcta gctaaagatg
     8761 tagatggaat ctatattcct actgataaca cgttcgcaag tgcggcaagt gttgtcggtg
     8821 aagtcgcaaa agaaacaaaa acacctgtcg ttgctggcgc agttgatcaa gtcaaagacg
     8881 gcggtctagc aacagtagga atcgactacg aaagcttggg caaacaaaca gggatcatgg
     8941 cagcaaaaat cttagacggc aagaatccaa gtgaacttcc tgtcgaaaca gcgaatgatt
     9001 tggaactgta tgtcaataaa gacatggcca aagcattagg catcaatcca gacagcatca
     9061 aagaagctga ataaaacaca ggaggattct catgacaaat ttgctctctc ttttcttatc
     9121 atctgcatcg caaggggtct tatgggccct acttgcgatt ggtgtatacg tcactttccg
     9181 tatcttggac attgccgact tgactgctga aggaagcttt cctttaggtg cagggatcgc
     9241 ggcagtaagt attacaaatg gctatcatcc attgacagct tgtcttctag gctttttagg
     9301 gagtgcggca gcagggcttg tttccggact gcttcacaca aaattgaaaa ttcccgccct
     9361 tcttgcaggg atcatcacca tgactggttt atattctgtc acatcccgtg tgatgggtgc
     9421 gccgaacatc tctttattgg gtcagccaac gatctttcat tgggtcgagt cattcggcat
     9481 aacaaaagac aacgctgtac tgatcgtcgg tctgcttttt gctttgatcg ttgtatgttt
     9541 acttgttttg ttcttacgta cagaaacagg acttgctatt cgtgcaactg gcgataatct
     9601 ggcaatgagc gaagcaaacg ggatcaacac agataatatg aagatcatcg gttatatgat
     9661 ttctaatgga tgcattgctc tttctggctc cttgcttgcg caaaacaacg gctttgctga
     9721 tttaaattct ggtattggaa cgatcgtaat tggacttgct tctattatca tcgcagaagt
     9781 tttgttacgt aaccagccac tatttctccg tctcttgact gtcgttttag gtgcggtcat
     9841 ttatcgtttt attttagcgt tagtcttcga actgaatgtt gaaccaagtg attcgaaact
     9901 tgcttctgct ttcgtgctag tgatctgtct ttctttcccg cagatccagc aaaaattcca
     9961 tttacctcaa aaaaagaaag gcggtagtgt caaatgaatg acgtcttaac attggaaaat
    10021 ctccatcata cctttgaaaa aggaacgatc aacgaaaatc atgtcctgaa aggcattaat
    10081 ctttccttag atcccggcga cttcgtgacg atcatcggtg gaaatggggc tggtaaatct
    10141 actttgctga acagcatcgc cggtacattt tctgtcgatc agggtcaaat tttattgaat
    10201 ggaaaaaata tcacgaaaaa atctgttgtc gaacgttcga aatccatcag tcgcgtattc
    10261 caagatccta aactagggac agccgttcgt ttaacggtag aagaaaatct tgctcttgct
    10321 atgaaacgag gaaaaaaacg cggcttcttc cgtggtgtca aaccacaaga ccgaacattt
    10381 ttcaaagacc agctggttcg gctaaatttg gggttggaaa atcgtttatc ctcagaaatc
    10441 ggtttgctgt ctggaggaca acgtcaagct attacgttat tgatggccac attacagcgt
    10501 ccagaactga tcttattaga tgaacacact gcagcgcttg accctgctac ttccaaaacg
    10561 gtgatggaat tgacagaaaa gctgatcact gagcagaatc tcactgcttt tatggtcact
    10621 catgacatgg aagacgcgat ccgctatggg agtcgactga tcatgctgca tcaaggaagg
    10681 atcgtcgtag atatccagca agaggaaaaa cagcagctca cagtgcccca attattagcg
    10741 ttgttcaaac aaaacagcgg cacggcatta aaagacgatc aagtactttt atcttcataa
    10801 tcagaaagag gatgggacaa aaaatgaacg cccaccctct tcttttttct ctttccttct
    10861 ttcgtttttg ctttatgtat caaccgttgt ttcttcgaaa actattttgt tctctccttc
    10921 ttcctgatag aatggatggg aaaggagctt gatgccatga caacgaaaga caaggtactt
    10981 caattattga aaaagacacc agattttctt tccggagaaa agttggcaca gcaactggaa
    11041 gtttcccgga caagtatttg gaaagccatc aaagaattgg aaaaggcagg ctatcgtttt
    11101 gagcacgggc ctacagggta tcgttacctg ccctctgacg tactagatgc aaaagaaatc
    11161 cagcaagagc tacggtcgtt tattcctgat ttgaaagtac aaacgatgat gacttctgaa
    11221 tctacgatga aagatgccaa actggcagca gctcaaggag attctgtttc gaagttattt
    11281 gttgctgata cacaagaaca agctcgtgga cgttttggac ggcctttttt cgctgaacct
    11341 ggacgaggaa tctacatgag tctcttgctc catccagaca aacggtttga tgaattgcct
    11401 caatatacga tcatcgcagc cagtgcctgt gtgaaagcca ttgaatctgt aaccggtcgt
    11461 tctgcttcga tcaaatgggt caatgatatc tacatggatg gaaaaaaagt ctgtgggatt
    11521 ttatctgaag cagtcagtga tatggaaagc ggcaggatca gtcatatcat cattgggatc
    11581 gggcttaatt tttctgttcc tcaaaatcat tttccagctg atctccaaga aaaagccatc
    11641 tctgtctttc ccgacggaca agctagtgtc acaaggaatc agctaatcag caaaatctgg
    11701 caagaattgt tcacttatct ttctcaccca gaaaaagcgt tggacttcta taaagaaaaa
    11761 tcattcgtat tgggaaaaac cgtgtcgttc aatcaaaaag gaacgactta tgtcggtctt
    11821 gcaaaagaaa tcggtgatca tggcgaactg atcgtccaaa caaatgaaca gaagatgagt
    11881 ttgtcttctg gcgaaatcag tctcacatct atccgtacgc aataattatc agatttcaca
    11941 aaaggacacc tgatctcata cagtgtcctt ttttctgaat tctcttcaaa agactctcct
    12001 tattctatca gctgacagtt tctctatcct ctagaaaaag gtattctaaa aatatctata
    12061 aagcatggta cactttcttt aaatgaatca atgcaaaaaa caagagagtc atcagtttac
    12121 acgatcaaaa gagatagcag gccgcttcct ttttcaatct tatagataga ccatgggtaa
    12181 cgataaaaac aaaaaagaac ttgcgactta ttttgccgta agttcttttt tagctagtgc
    12241 cttaatctct taatcccaac aaacgtctcg catagatcac ctgaccagag tgcatagcag
    12301 catcatcaat aatagaaacg agacgcattc ctcgtgtgac tggtggtgtc cagttctcgt
    12361 caattacttc atctagctcg gaaggttcca gccttcttaa gtaagaaata gcaaaatcag
    12421 cagctcctcg caagtatcct agcagatctt ctatttgatt agcttggatc tgctttgctt
    12481 gttccagact atgcttccaa tcttgttcgt tatctgctat cgtcatcgga aaccggcttt
    12541 cccagtcttg actatgccaa attggagaag ttcctgctaa atcagcaatc tggaaatcta
    12601 attctcgagc ggtatgccaa gccagccaag tcattgattt gatctgcggg gctctatctt
    12661 ccactggaaa acgattggcc tcctcaatag agacgccttc aaacaaacgt tcaaaccgat
    12721 cgtatgcccg atccacactc tcgcatagca tatctaaata atccatttat tttcctcctt
    12781 tttatttatc ctacttactt tttcctaatt ctaggatacc tacttatatg ttactacaca
    12841 cccttatttt ttacacttta acagtcacca tcatcaatag tttggagatt gaaagaaaaa
    12901 gatagtgtcg aactatcaat tgaacaagaa gcaacaatat gaacgtttca ttaatattct
    12961 ttaaataaac aatgattttt caaacgaaca gtttaagact aggagaaaaa gagtataata
    13021 ggaaaatgtt tgaaaggaaa ggaaggggaa aaatgaaaaa aagggcaagt ttactagtcc
    13081 tagttgcttt gttgttcaat atcttttcgt atggtgtggc cgtttcagcg gaaacataca
    13141 ttcctaaaga ccctgggaat atttataatg atctaactgg taactatggc ttgttaggaa
    13201 ttgctagtca attccacgta tttgctaaaa ataagacaac tgtgaatgca catacagacg
    13261 gaaatatagc aacacatgaa cttgatgcaa aaaataattt tggtacagag cttcatgaag
    13321 gtttattgaa ccaagaaata aattatatac aaaaaactga ttctttcgct aattcttcag
    13381 gcatacctac aggtagcaca agaatgaata aatttgtcgt tggcagtaac gtggatgtag
    13441 cacttcaaga tggtcatcct acagtcaatg gatccaggct tgatcactta actccagatg
    13501 atttttataa agatcgtgct gggcatgaat atattgattt cgagcaggaa tttgctaaat
    13561 tgaatgtagc agcaaatgat cttgcaacga tcactccagc taaaacatat actgctgcag
    13621 attttccaga catgaacaat cggacaatcg atttaactga tttatcagat accgggtttt
    13681 tattggtaaa tatcgatgca gaagtgttaa cgatgaatac gccacttcaa atcattaatc
    13741 caaatgatca agtagttgta tttaatgtca tcaacagttc ttctgcattg aacgtacagt
    13801 caccaatcaa atacaatgat cgttcaaatc atgaaacaga agatttttcg gatgcgaata
    13861 tctcttggaa ttttggaaat gagatgactg atttgacaat cagtgctcct tttcaaggaa
    13921 caatccttgc gcctaatgct actatccgca ttactcaaaa ccaagacgga acgattatag
    13981 gaacaaatgt gattttagat gcagctacca atcgttggga tcctaacgaa atatttatca
    14041 gcgacaatgg tacagatacg acagacacaa gcgacagtac agatacatct accacaagtg
    14101 atagctcaga ttcatca
//
LOCUS       DBNICI010000067        13927 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.067, whole genome shotgun sequence.
ACCESSION   DBNICI010000067 DBNICI010000000
VERSION     DBNICI010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.067"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(56..880)
                     /locus_tag="AC1ZCC_001584"
     CDS             complement(56..880)
                     /locus_tag="AC1ZCC_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIQ1629842.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(853..1533)
                     /locus_tag="AC1ZCC_001585"
     CDS             complement(853..1533)
                     /locus_tag="AC1ZCC_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HIQ1629843.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1556..2494)
                     /locus_tag="AC1ZCC_001586"
     CDS             complement(1556..2494)
                     /locus_tag="AC1ZCC_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HIQ1629844.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2739..3590)
                     /gene="ispE"
                     /locus_tag="AC1ZCC_001587"
     CDS             complement(2739..3590)
                     /gene="ispE"
                     /locus_tag="AC1ZCC_001587"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HIQ1629845.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3744..6317)
                     /gene="mprF"
                     /locus_tag="AC1ZCC_001588"
     CDS             complement(3744..6317)
                     /gene="mprF"
                     /locus_tag="AC1ZCC_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HIQ1629846.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFSEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6458..7264
                     /gene="proC"
                     /locus_tag="AC1ZCC_001589"
     CDS             6458..7264
                     /gene="proC"
                     /locus_tag="AC1ZCC_001589"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIQ1629847.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7343..8899)
                     /gene="rny"
                     /locus_tag="AC1ZCC_001590"
     CDS             complement(7343..8899)
                     /gene="rny"
                     /locus_tag="AC1ZCC_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HIQ1629848.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9310..10359)
                     /gene="recA"
                     /locus_tag="AC1ZCC_001591"
     CDS             complement(9310..10359)
                     /gene="recA"
                     /locus_tag="AC1ZCC_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIQ1629849.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10468..11709)
                     /locus_tag="AC1ZCC_001592"
     CDS             complement(10468..11709)
                     /locus_tag="AC1ZCC_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HIQ1629850.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11949..12527)
                     /gene="pgsA"
                     /locus_tag="AC1ZCC_001593"
     CDS             complement(11949..12527)
                     /gene="pgsA"
                     /locus_tag="AC1ZCC_001593"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIQ1629851.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12736..13659)
                     /locus_tag="AC1ZCC_001594"
     CDS             complement(12736..13659)
                     /locus_tag="AC1ZCC_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HIQ1629852.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
BASE COUNT         3985 a         3099 c         2433 g         4410 t
ORIGIN      
        1 aaaaagaagc ctataaaaaa tttttcgttt ttataaggct tcttttcttt ataatttagt
       61 tattttttct tctgaaggca accaatattc gtttgatgat atttacaatc aggaacaaag
      121 caataaagac taatgtgatt gttgctcctg gaggtgtatc tagataaaac gagctagtta
      181 gtcctgacag catccctaga aatcctacac aaacactgat agcaatcacg acattgaagc
      241 ttttgcctaa tcgcataccg attgctgcgg gaagaaccat gattgcggat atcaaaagtg
      301 caccggcaat cgggatcatc acagcaatag ccacaccagt aatcacattg aacatcatcg
      361 acatcaaatg aacgggtagc ccatccacgt gggcggtatc ttcatcaaaa gtcagaacat
      421 acatcggccg tttgaataaa aagaacaata taccgatggc aacgaataag attcctaaaa
      481 agatgacttg ctcccatgta atggtcacga tcgaaccaaa cagataggat tggatacttg
      541 ttgctgaatt ccctccgctc aaattcatca atactaacgc tagcgccaaa ccaccagcca
      601 ttaaaatggc aatggagatt tctgaatagg ttcggtagat cgttcgcaga tactccaata
      661 tcacagctgc taatacaacg actagcatgg tcgtaagtgt aggattaagg ttcaaaaaga
      721 atcctaacgc tacccctgct aaagagacat gagaaagcgt atcagccatc agcgattgtc
      781 ttctgatcac taaaaacaca cctagcatcg gggcgattcc ggcaatgaag atagcggcaa
      841 gaaacgctct tctcatgaat tcatatgaaa gcaacgccat tgagaatcct cctttctcac
      901 cagtcggatc tgtctgtccg catactgttt gatatcttca tgatcatggg tgatcattag
      961 aactgatttc ccatggacat gcgcactatg tcggagaaga cgataaaatt catttctgga
     1021 ttcttcatcc atacctgttg ttggttcatc aagaacaaat aagtcagggt cagtagcaaa
     1081 aatacgcgct aaactgattc gctgcttttg tccgccagac aattcaccta ttcgccgatt
     1141 tcgcatatcc cacatcccta cagcttctaa agcacgctgt acatgttgat gatctttttc
     1201 agagagacgc ttgaaccagc ggcctcttgg atatcttcct gaacggacta attcgatcac
     1261 cgtactagga aaacctgcat tgaaagatgc aacttgttga ggaatataac cgatacttaa
     1321 tttttttcct tcttgatttt tctcagcaat ctttatcatt ccttttgaag gtttcagtaa
     1381 tcctaacgat gctttcacca aggttgactt ggctgctcca ttttctccag ttaaaatgac
     1441 aaattcaccc gcatctacat gataagagac atcttctagt acaggttcat catcatagta
     1501 aaaggtcaaa tcagatattt cgatataacg caccttacaa cctccattct aaaacttaat
     1561 tgatactttc ttttaaagct ttcagatttt tttccatgac agaaatataa ttttcgcctg
     1621 ctttgatttg ttcattagtc aagctttcta aaggattcaa cacttctagc ttcactcctg
     1681 tttcattcga caatgtctca gctaccttcg aagaggcatt ttcttcaaaa taaatcactt
     1741 tgatgttgtt tttcttcaca aattctttta gctcagctaa gcgacttggt gtgggttcct
     1801 gatctggtga aacgcccgaa atcgctactt gttttaagtc gtattgattc gctaaataag
     1861 caaaagcagc atgctgagtg acaaatgtgc ggtttgttgc atcttttagc tcatcggagt
     1921 atttttgatc cagtgcttca agctttttga tataagcatc ggcattcttc gtaaaagttt
     1981 cctttttatc tggatatttt tcgcctaatt cttcagcaat cgttctgact tcttttatag
     2041 ctaataccgg atcgagccaa acatgtggat cgtattcatg tgaatgacca tgcccttctt
     2101 ctgattcttc atgttcgtct gcttcttgcg attctaaacg gtcgattcct ttagctgctt
     2161 cgatcatcaa ggtctgtttg ctatccactg aatcctttaa cgaatccaca aaaaattcca
     2221 tgtctgaact attatacacg aaaacatctg catcggaaat ttctgctctt tcttttgcac
     2281 ttggttcgaa atcatgcggc tccgttccag cagggatcaa aagtttcacg tttccttcat
     2341 cccctacgat ctcttttgta aaatcataca tcggataaaa agtcgtaacg atcgtcaaat
     2401 cctctttttt gtctgcttcc ttgttcgtat ttccacatgc tgcaaacagt aaagcaccta
     2461 tcattaccgt agctgtcaaa agatattttt tcatttatat gctcctttca atgaacaaat
     2521 cggaatttta cgatttatta acgtgaacta atgtatcaaa ataaaaaaac acagtcaata
     2581 gaaatccatc aatttctgat ttaagcatgt atattcttga ttaaatcgta aaatggatga
     2641 gttgctgctc atatctagcc atttcactac aataaaaaac tgaagaatac cattttagga
     2701 ttcctcagtt tttcatcacc tattttactt cttatatttt attgcagggt tcgtactaaa
     2761 taaacttcct cacaaaaacc ttttaaccca ttatatactc tctgcgctct tgaataatgt
     2821 tgacacaagg caaaaacggt tggtccgctt ccactcatca atgcagcatc tgctccgtac
     2881 ttgatcatac gctctttgat ttgatggatg actggatgcc gctttcccgt tatatcttct
     2941 aaactgtttc ccattttttt gatcatctgc tgataatctt gctctcggat ggcttcagca
     3001 agtccgtgga tgtcttgatg ttggatggct tgcgtatcta cttctctaaa cactgtacgt
     3061 gtagagacac tcattcttgg tttgaccaac acaacccagc actgtggcat agaaggtaat
     3121 acttggattt cttccccttt gccaccgaca aatgccgtac ttccgtagac acagtaagga
     3181 acatctgtac cgacttccaa accgatttct gccagttctt ctaacgaaca gcccagattc
     3241 cataattggt tgatccctct tagagcagct gcacagtctg tacttcctcc gcctaatcca
     3301 gctgctactg gaatattctt cttaacaaag atcttgacac cggttttgat attgtattta
     3361 ttttttagca ataaagcagc ttgataaaca ttgttccgtt catctaccgg taaaaatgct
     3421 ttatttgttt ctacaatgat tttgtcttct gttatttctt caaaaaatag atgatctgcc
     3481 aagtctacgc tcgacatcac catttccaac tcatgatacc catcttgccg tctatataat
     3541 acatccagtc ctaaatttat cttggctggc gctttttcaa tcaattccat cctctcaccc
     3601 attcgaaaag tctaagtcgc aagtattcta tcatagggaa aaataaaagt atagcttatg
     3661 tctgcctagt ggttgatttt gtcatcgttt caacagcttt cacagaattt tctcttcata
     3721 atccaaacaa gttctagtga ttcttataat tctgtgagca cttcttctgc caaactcgga
     3781 acatttcctt tttcttttcc tatcagactg agtagctgca tggtagcaaa tccgaaatta
     3841 cttcctttcc tataagcaaa gtaacgtcct tcccaatgat cgacatattt taatttcttt
     3901 tcataagcag actggaagga atagaaaaaa ttaccataat tatatacgat atgcatgact
     3961 ttttcttgaa agaaagaaaa atctgtttct ccaacattag ctaatggagc taagcctaga
     4021 ttggcttccg agtagcccat tttctcgtat acaataaaca gattggctaa cagatagtca
     4081 gctaattcct cagattcttt aggtaagacc cgcaacaaat catatcccgc ctgcttcttc
     4141 gtcatcggct gagcggtgat gaagccaacg actttttgat ctttacggac aataccgaca
     4201 ccgctagata atacatatga tttatcaaat cgtcccacag agaagtattt ttctcgtgca
     4261 ccttgaagcc atttttgtga aacttcattt acagcttgat gtaattcttc tggcagttga
     4321 tcgtaccaag taaaatggta tccttctttc atcaaacgtt gatattcaag ttgctgactc
     4381 agccgagaca agtcttctgt tgctagatca acaatccctg cttcacctac tttcgtgaag
     4441 tgaaaaccca attcatgaag catcactacg tagctttctg atatcttata aaacgcaagc
     4501 tgatagccta aacaatctgc ttgtttcata aactgattcg ttgctgcccg catcatttta
     4561 ggatttccaa ctggatctcc taaaacaaag catttgtttc ctttgatctg ataaccaaaa
     4621 acgacttgat cttggtcatc ttcttggtaa taaaaataac tgtaacctga caagcgaagc
     4681 tgatggcttg tagctgtgcc tccgtatgtt tccaataaac ggtcaaaacg gtctttttcg
     4741 tactcactgc caagcttcgt agtatcttca gctaaatatt gataaagcgc cactaaagct
     4801 agcaatgaga tgcccaaacc aataagccct gagaaccaca catcatctga aggaaacaaa
     4861 ataaaatggt ttcctgttat tcgaccgact aaaaagcgtc cgctgtgata gcctgcaatt
     4921 gcgtaaacga taaacaaaaa gccgaacaag ctagcatcaa agattatagc tccccaagaa
     4981 taaacgaatt ttgttcgata aaattctttt ctggataacc acacagctaa cagaatcaag
     5041 acatatacca atatcaactg ccaagataac gtacgtgtga ttgtatttaa aataccaaaa
     5101 gatagtaaag caatcgtagg cagaaatgcc tttttgactt ttactgaaag tgctctagca
     5161 aggccgagta ataaaaagcc aaccaataga ttcactgtct gatctaaaaa attgaatgag
     5221 aacggcaata atacttgaaa tagccgactg attgcagaca gattagtgat tgttgataga
     5281 agtaccatca tgattcccgc aaaatagaca gcacttacga gcacgatatg cgcaaccttt
     5341 tggaaaaaca gtcgcggaat gttatctaaa aatcgattga ttttgactcc tgtatgtgtg
     5401 atgaacaata gaagtcccgt cataaaaggt agaacataat aaaacaaccg ataatataat
     5461 agccaaacta ctgctacatc ttgccgaaca gacaattgac tcattcctaa gatcatcaaa
     5521 acgtcaaaag tccccattcc ccctgggacc atcgtcagca ttcctatcaa tgtagcaatc
     5581 atgaacatag gatagatcga aagcatcgaa atatctattc tcatcaactt gccgatgatc
     5641 aaaaataccg caagggctcc cgtccattgt ccaaaggatg ccagtagcag accaagcaag
     5701 ccttttggat aaaactcaga gaacaaggtc tttctcttta gataagcaaa aaagaaaatg
     5761 actggtgcta aaaaactacc gccgaaaagc cagatccaat agcttcgaaa aatactttct
     5821 gactgaataa aaaagacatc aataaatgta agaaacgacc atacagacaa accagtcagc
     5881 ataaatagtg cgacttttga tacagtggct aaaaccattt ttctatttgt ctgctttcca
     5941 taaaaactag ctcgcaggct tgctcctacc acaccaccaa aaccggcgag attgttgatc
     6001 gtgtttgtta cccacgcagc aagaagaaac tcttttcttt ccatttttgg ttttccttgt
     6061 ttttctaaga ctctgatcgt cacccaatca tacaacaaca tcggagtgac acccagcagt
     6121 ccagccagaa tcatgagcag gatcgtcatg cggctttgtt gtcccatcct ttgccaaata
     6181 tcttgccaac tcatgccatt agcaatattt gcgacttgat ttgccacaaa gactaacacg
     6241 gagccgaaaa aaatcagttt taaaatcaga ctatgttcct ttatccattg cagcatcaag
     6301 tatcgtttat ttttcacatt ttcccattcc taacttgtat acttttgtgt cataccccaa
     6361 tgttatctta tttattttaa tttttccact ttagacctgt tctttgcttg cgatgataga
     6421 aaaagaaaga tatcctaatg ctaaaggaga ggataacatg aaagtaggat ttatcggcac
     6481 agggaatatg gcacaagcta ttattgatgg tttattaaaa aaaggaacag tctcgaaaga
     6541 agaggtgttc gtttccagtg gacattttga aaatgcacaa aaatttgctg ctgaaactgg
     6601 cgtccatgca tgcaaaagta atcaggaagt tgcatcacaa tcagatatcg ttcttttagc
     6661 cgtgaaacca aaaatcattg aaaaagtatt ggaagaacta agagaatata gccagaaagt
     6721 cttattcatt tctattgctt caggaaaatc attaatagat ttagaaacag cgcttgaaga
     6781 tcctcaggcc tccatcgtac gtgtgatgcc gaatgtcaat gtcagcgtag gagcaggcgt
     6841 ttctgcgatt tgcaaaaatg aagctgccac ggaggaacag cttcagcaag ctacgatgat
     6901 ttttgaagca atcggcagcg tctatccttt agcagaatca gatttcagta cattcattgg
     6961 ccttgctggc agctcacccg cattcgttta catgctgatc gatgcgatgg gacggactgg
     7021 tgtactgaat ggtttgtcca aacagacagc cactgagatt gctgcaaaag caattatggg
     7081 ttcttgtcaa aaattccttt caagtgataa aaatccatgg gaacttgtag accaagtttc
     7141 ttctcctggc ggtacaactg ttgcaggtgt agttgcctta gaagaagcag gattcatccc
     7201 agcaatcatc aaaggaatca atacgacgat cgagaaagat cttgagatgt tgaataaaaa
     7261 ataatgatag caatgttttt tcgtgcctta caaaaaaaga agagctgtgg cttttgccgc
     7321 ggctcttctt ttgtattttt tattatttag cataatcagt tgcacgagtt tcccgaataa
     7381 cgattacttt gatgtgtcca ggataatcca gttcttcttc gattcgttta cgaatatcac
     7441 gtactaaacg aacagcgtct aaatccgaga tttcttctgg cttgaccatt acccgaactt
     7501 cgcgtccagc ttgaactgcg taacttgatt ctacaccggc gaagctgttc gaaatatttt
     7561 ccagacttct taaacgacgg atgtagtttt ctaatgattc acttcgcgca ccaggtcttg
     7621 ctgctgagag cgcatctgct gcagctacaa ggacagagat aacagaagta gcttccacat
     7681 ctccatgatg agaagcgatc gcgttgatta ctactgggtt ttctttgtac ttggcagcta
     7741 attccgcacc gatctcaacg tgagatcctt caatctcatg gtcaagtgct ttcccaatat
     7801 catggagcaa cccggcacgt tttgctaatt ggatatcttc gccaagctct gctgctaaga
     7861 taccagctaa ttttgcaacc tcgaccgaat gcttcaagac gttttgtcca taactagttc
     7921 tgaatcgcag acgtccgatg atcttgatta gatctggatg aagtgtatga gcaccgactt
     7981 caaacgctgc ctcttccccg tattcgcgaa tacgttcatc catttcttta cgagatttct
     8041 cgaccatttc ttcgatacgc gctggatgga ttcgtccatc ttgtatcagt ttttccaagg
     8101 tcatccgtgc gatttctctt cggattggat cgaatccgga aagaacaact gcttccggtg
     8161 tatcatcgat gattaaatcg ataccagtca gcgtctcaag cgttcgaata ttacgacctt
     8221 ctcgtccaat gatacgacct ttcatttcat cattcggaag ggtaacgact gaaacagtcg
     8281 tttctgaaac ttgatctgct gcgcagcgtt gaatcgctaa tgataataag tttttcgctt
     8341 tgcgatctgc ttcttctttg gcacgctgtt cagattcttt taccattaga gttaactcat
     8401 gattcagctc ttcttcagtc gatttcatga tgacatctcg tgcgtcctct tttgataaac
     8461 cagcaatttt ttctagttct ttttgttgat cttcaatcat tttttcaacg tctttttctc
     8521 gctcttcaat taattgctgt tttgaactaa gtgcttcttc tttactttca agtgagttct
     8581 cgcgtttttc taacgagtca tctttacgat tcagcgtttg ttcgcgttga atcaaacgat
     8641 tctcttgaga ttttaattct aacttacttt ctctcagctc actttcaact tcagaacggt
     8701 atttctgatt ttcttcctta gcttctaaca atgcttcttt ttttagagtt tctgcttctt
     8761 tttttgcact atcgatgatt ccagcagctg atgattgtgc accagctatt tctttctcgt
     8821 gacgtgactt tgcgaccata aaccctaaac caagaccgac aattaaaccg atgatagcga
     8881 ggagaatgtt gaataccata aaatccacct ccatactatc tttttcattt ttatcatcta
     8941 tacagcttct atgataaatt attctaaact cgtcaatatg catcttcgca tatatgtgta
     9001 caaaaaacat gcacaacttt attctaatgt ttgtaccccg tagtgtcaac tttaaataaa
     9061 caaagaaacg gctttctact tattcattaa acgatggttt ttaaaataaa tgacactagt
     9121 ttcatatagg taaaccttta aattgagaaa cttttctatt aaattattct ttgtagatga
     9181 acagatattt ttattttatg tacccactcg tataattaaa taactcacga tttctttatt
     9241 caataaaaaa acggcaggtc aaggagagta cctactcttc agaccagccg tttttgttta
     9301 tcataatttt tattcttcta ctaaaggcaa ttcttcttgt cctttttcat cttctgggac
     9361 agcgacttct tcaccaatac cataagctgc tctaactaga gcagaaactt ctgccatcat
     9421 ttcaggatga ttagccatat agattttagc gttttcccgt ccttgaccaa tccgatcttc
     9481 tttataagaa taccaagcac cgcttttatc aacaatgtct ttttctactg ccatgtctag
     9541 aagttctcct tcttgtgaga tccctaaacc atacatgaca tctacttcag cgattttgaa
     9601 aggaggtgct actttgtttt ttacgacttt gatctttgta cggttaccaa caatatctgt
     9661 accttgtttc aattgttctg cacgacgtac ttccaaacgg atcgttgcgt agaatttcaa
     9721 tgcgcgtcct ccaggagtga tttctggatt cccaaacatc acgcctactt tttcacggat
     9781 ttggttgatg aaaatagcga ttgtttttgt tttgttgatc gaaccagaga gtttacgcaa
     9841 tgcttgagac atcaaacgtg cttgtaaccc gacatgtgag tcacccattt cgccgtcgat
     9901 ttctgctcgt ggaactaaag cagcaactga atcgactact acgatatcta cagccccact
     9961 tgatactaaa gcatcagcga tctctagacc ttgttctcct gtgtcaggct gtgaaagaag
    10021 tagttcatcg atattcacac ctaatttttg tgcatattgc ggatctaacg catgctcagc
    10081 atcaatgaaa gcggccgttc cgccattttt ttgtacttct gcaatagcgt gtagtgcaac
    10141 tgttgtttta cctgaacttt caggtccata tacttcgatg atacgtccac gggggtaacc
    10201 gccgacacct aaagcgacat ctaacgcaag agagccactt ggaattgtag agatttgttg
    10261 gtcgattttt tcgcccaatt tcataataga gcctttacca tagctctttt caattttttt
    10321 cagtgcagca tctaaggctg ctttacgatc atctgccaat acgataatcc tccttatatt
    10381 tacaaatcag agcaacattt cattatcctt atcatatctt ttttagtgaa aaaaagcaag
    10441 aaaaaaccga acaaatattc gcatttatca ttttcttaaa agctgttttc tcaaaagatc
    10501 aaatccttgc ataaccgcat tatatctgat agcagcacga tctctggcaa agtgatattt
    10561 ttccgaaacc acgccgtctt tagatgaaat agcgatccat actgttcctg cagattgacc
    10621 ttctaacgtg tccggtccag ccactccggt gaaagctaca ctgtaatctg ttcttgctat
    10681 ctcacgtgct tgaatggcca tctgctccac acattctttg ctgactgttc catattctgc
    10741 taatagctcc ggatcaatct ccagaaaacc agcttttgtt tggagcgagt aggtaacgaa
    10801 tccgcctgga aatacttcag atacaccagc aatgtcgcct aatgcggctt ggaaggcgcc
    10861 ggctgtcaaa ctttccgctg ctgttacagt ctgcttgttt tctttcagta gttccactac
    10921 gacttttgct agcgaattat catcgccgta tccataaaaa tattccccta ctcgttcttg
    10981 gattttttct tctaatgcta aaagagcttg attccctgcc tgaacatcat ttgttttcac
    11041 tgtcagtcgt aatgtcactt catttggttt ggcatatggt gcgatcgttg gattgatctg
    11101 tgtctcaatc agatctttta gctcagtgac cagtcgcgat tcaccaatcc cataaaagcg
    11161 caaaacttta gaaattagtt tttcttcaga aggaaaacgt tcctctagta aaggacgtac
    11221 ttgttccaca aacattggtt tcaactcatt aggcggaccg ggaagaagga tatacgcatg
    11281 tgtatctgtt tgataaaaag tccctaaagc aagccctgta cgatttggta gaggaacccc
    11341 gccttcgata atctgggact gttgcagatt gtttttggtc atttttcgat gggttgtttc
    11401 aaaatatgcc aataattttt tgtatccttc cgtattttgt atgagagact taccgaggtg
    11461 agccgcagtt gcttcttttg tcaggtcatc ttctgtcggt cctaatccgc cacataagag
    11521 tatgagttca cttcttgttt cagctagagc aatcaactct tctaaacgct gctgattatc
    11581 tcctacaact gtttgataat aaacttcgat acctagatca gctagctgtt ctgacaagaa
    11641 agtggcattc gtattcacga cttgtcctag taaaagttct gttccgacag ctattatttc
    11701 tgctttcatt acttcccgct cccttatata gtatacgctg tatttttata tttttttatt
    11761 taaaaagagg gcaaggagct gtgacaaaaa tcttgtcaca gctccttgtt ccaaataaac
    11821 agtgttctcc tgccaaaacc tcggtcataa gtcacaatat acataaaaca tgaaaagctg
    11881 ttttttgaaa ttatcccttg tgactcagag ttagacggca ggctcacaac ctctccacta
    11941 aacgatactt acattgatcc tttgaacaca ccgctgtttt tgatgaagta atccacacct
    12001 gaatagatag taaagatcag acaagcataa agcatgattt gatctactgg aataccgatc
    12061 aggctgaatg ggatattatt gataaacagc aagataatag caaccatttg tgtagccgtc
    12121 ttgacttttc ccggccaagc tgcagccatg acttctccat gttcgacaag tagtaaacgc
    12181 aagcctgtga cggctaattc acgacagaca atgatcgcta ctacccaacc aggggccttt
    12241 ccttggtcaa ccaacatgat aaaagcagtc ataactagca ttttatctgc caacgggtcc
    12301 gcaaacttgc caaaattagt gacaaggcct cgcgcacggg caatttttcc atctaaccag
    12361 tctgtgatac tagctaaagc aaaaataact gcacctacta gctgtgtgac agccagtgtg
    12421 gtgtctccta cgttaaattg tccccaatct aaaggtaaac ctacaaccaa aatgaaaatc
    12481 gggatcatac aaattctaag tacagttaat ttatttggta aattcacact atcgactcct
    12541 ttcttcccta ttctacgtga ctatcagcga agtttccacc gatattattt acttttccaa
    12601 atgaatttat tcaaaggagt atttttattg aaattgatct ttttgaatta acgaagaagg
    12661 accgcgacac agatttgtca cgatccttct tttttatacc tacaagtaaa cagatgcgta
    12721 aactaattta tacgatcatt ctgcgtattg aagagtaagg ttcacatttt ttggactctg
    12781 attgttttcc cctggattaa ctggtacgga ttctccattc gctttgatcg ttgcattgct
    12841 tgctgcacca atcgtgatgg tcgcatttgt tgctgtttcc ggtaacacgg tactctgtgt
    12901 ttcatttgct gccagtgtcc cttgataaac ataggcattg tctactagca cacctaacca
    12961 tacacgattg acagcagtaa attcaagggt tacaggcttg gtagcgtctg tgatatttac
    13021 tgcgatggca ctgccagaat cttctcctgt agctattgcc attttctttt cttcacttga
    13081 ttttgttgtt gattctactg tagaagactc tttggtctgc gtgctttgct ctgaggaaga
    13141 ggactcctcg actgatcctt ctactttaag cgaagaagct gtgtctccga ttattggatc
    13201 agcttgacga tcttgccaag tcatatagcc cacgacaacg acaatcgcca aagcaaccaa
    13261 tcctaataaa atgacaggaa gacttgtcca aaagcgactt ggatgcgatt cttcctgatg
    13321 cattgctttt ctagaagtac ttactgtttc aggctgcgga cgttcaggga ccgatcctgt
    13381 caatttgctt ttcccatcaa aaacatcgac taaatggtct ccatcttctc ctacagcttc
    13441 agcgtattgc cgaataaatg cccgcacgta aaatttacca ggcaattggt caaaatcatc
    13501 attgtcgatt gcttccaaat aacgtttttg aatttttgta atctgctgta attcatctaa
    13561 agatattttt ttattcaggc gtgcttgccg taatttttct ccgatggttt caaaagccac
    13621 tcgttcatct tctccagtct gtttgattct ccgaagcatc tgctgttgca acgaaagacg
    13681 ttatgtttgg gtgctgtgac aaaagtcatg tcacagctcc ttttttccga ataaacattg
    13741 ttctcctgcc aactcctcgg tcacaagtca caatatccaa taaatataag aagctgtttt
    13801 ttgaacttgt tccttgtgac tcggagttaa acggcaggct cacgacctct ttcttaaacg
    13861 gtgttcaatc agctgttcct cggtattagc tcaaaaaccg gcaaaatata aggagatatt
    13921 tttccgt
//
LOCUS       DBNICI010000068        13885 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.068, whole genome shotgun sequence.
ACCESSION   DBNICI010000068 DBNICI010000000
VERSION     DBNICI010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.068"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(175..1461)
                     /gene="yfmH"
                     /locus_tag="AC1ZCC_001595"
     CDS             complement(175..1461)
                     /gene="yfmH"
                     /locus_tag="AC1ZCC_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P 5-aminopentanol modification-associated
                     protein YfmH"
                     /protein_id="HIQ1629853.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1454..2722)
                     /gene="yfmF"
                     /locus_tag="AC1ZCC_001596"
     CDS             complement(1454..2722)
                     /gene="yfmF"
                     /locus_tag="AC1ZCC_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P 5-aminopentanol modification-associated
                     protein YfmF"
                     /protein_id="HIQ1629854.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2974..3435
                     /gene="mscL"
                     /locus_tag="AC1ZCC_001597"
     CDS             2974..3435
                     /gene="mscL"
                     /locus_tag="AC1ZCC_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HIQ1629855.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3494..3940)
                     /locus_tag="AC1ZCC_001598"
     CDS             complement(3494..3940)
                     /locus_tag="AC1ZCC_001598"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1629856.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(4033..4278)
                     /locus_tag="AC1ZCC_001599"
     CDS             complement(4033..4278)
                     /locus_tag="AC1ZCC_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HIQ1629857.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(4393..4971)
                     /locus_tag="AC1ZCC_001600"
     CDS             complement(4393..4971)
                     /locus_tag="AC1ZCC_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIQ1629858.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(5048..5578)
                     /locus_tag="AC1ZCC_001601"
     CDS             complement(5048..5578)
                     /locus_tag="AC1ZCC_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIQ1629859.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(5582..6442)
                     /gene="rlmB"
                     /locus_tag="AC1ZCC_001602"
     CDS             complement(5582..6442)
                     /gene="rlmB"
                     /locus_tag="AC1ZCC_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIQ1629860.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(6435..6830)
                     /locus_tag="AC1ZCC_001603"
     CDS             complement(6435..6830)
                     /locus_tag="AC1ZCC_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HIQ1629861.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(6827..8239)
                     /gene="cysS"
                     /locus_tag="AC1ZCC_001604"
     CDS             complement(6827..8239)
                     /gene="cysS"
                     /locus_tag="AC1ZCC_001604"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIQ1629862.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(8236..8772)
                     /gene="epsC"
                     /locus_tag="AC1ZCC_001605"
     CDS             complement(8236..8772)
                     /gene="epsC"
                     /locus_tag="AC1ZCC_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HIQ1629863.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(8858..9097)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(9094..10554)
                     /gene="gltX"
                     /locus_tag="AC1ZCC_001606"
     CDS             complement(9094..10554)
                     /gene="gltX"
                     /locus_tag="AC1ZCC_001606"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIQ1629864.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(10724..11881)
                     /locus_tag="AC1ZCC_001607"
     CDS             complement(10724..11881)
                     /locus_tag="AC1ZCC_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HIQ1629865.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(11915..13285)
                     /gene="radA"
                     /locus_tag="AC1ZCC_001608"
     CDS             complement(11915..13285)
                     /gene="radA"
                     /locus_tag="AC1ZCC_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIQ1629866.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(13348..13875)
                     /locus_tag="AC1ZCC_001609"
     CDS             complement(13348..13875)
                     /locus_tag="AC1ZCC_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HIQ1629867.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         3644 a         3030 c         2490 g         4721 t
ORIGIN      
        1 attacttcgt ttgattttat tttaatataa gttgattaaa agagagcatg atttgtttta
       61 ttttctcact aatttttcca acacaactaa gaataccata aaagctgatt ttggaggagg
      121 ttcctactac aagtctaaaa cgaaatttaa gacttttcct cataaaatac tggtttattt
      181 tggtctcatg taaaactcgc tgaaggcctc ttcacttata aatgcttccc cagctgccag
      241 tacgtctttc atttggatcg aatcgatgat ttcaggaaga tcaaataaag ttcgatcgcc
      301 aaataaactt tgcgtaaatt gattggcaat atattcgatc gaatttaatg attggaaata
      361 ttgtccaagc attttcttct tcagcaaatc gagatttgtt tctgacactt ccggatcatc
      421 agcgaatcca agaatgattt ctttgacttt ttctgctgct ttctctggtt cgtccgtatc
      481 tccgctgaag tccgcaaagt gaaattcacg atccaagctg aattcaaaac caaagctgtc
      541 atcgataatt ccttgattgt acatcgctag atagtttcga gaagtattgc ctaaaatcat
      601 ttggaacaac agattgattg ctgttttata gcgtaacagt tctgttcctt cttgcggcaa
      661 tgtgtctaat cctttaatac caagtacaaa tttatctcgt gtaatggctg cttccaatgc
      721 actttgtttg atgatttctt ttgtgttttc agggaaataa cgtacaattt cttgctttgg
      781 agggaaattt tttgcttctt ggttttcccg aataagtttc atcaatttct caggctccat
      841 cttaccaact acaaataaaa ccatattgct tggctgataa aacgtccgat agcacgtata
      901 caagtcttga gctgtgattt tgtcgatact ttcaaccgtg cccgcaatat cgatatgcaa
      961 tgggtgtgta ggatacaagt tatttagtat gccaaaaaac atccgccagt tagggtcgtc
     1021 ttcatacatt tggatttcct gaccgatgat tcctttttcc ttgtttacag tttcttctgt
     1081 gaagtaaggt gcttgtacaa aatcgatcag agtcgtcaga ttcttttcta cctgatccgt
     1141 cgtagaaaac agatagctgg ttttcgtaaa acttgtgaaa gcattggcgg aagctccttg
     1201 tttcccaaat aattgaaaga catcaccatc ttctttttca aacaacttat gttctaaaaa
     1261 atgggcaatt ccatctggaa ctttcacctt ttctttttca ccatagggaa taaattcgtt
     1321 atcgatcgaa ccataattcg tactgaacaa accatatgtc ttgtgatagt catttttagg
     1381 caacaaatac acggtcaaac cgtttggcag tacttcatga taaagtgttt cattgatttg
     1441 ctcatattct gttttattca tttgcatttc ctcctgctaa aaagaagatt gcctgcaatt
     1501 cgatttgctc agctacttgt tgtatctctt tgatcgttac gctctccata cgtttcaacc
     1561 attcttcatc agataatttt gtttccggca gccagctgtc caaaaaagaa gcttcgattg
     1621 ccgcttgcgg agagtctaga gaaagcagat attggtttct cagcattgct tttgtctgag
     1681 acagttccaa ctctgttatt tcaccatttc gcaaactttc taattgctcg tgaatcagat
     1741 gaagtacttg atttcggttc ttttcatcga taccggtttg gacactcatg aatcctcgga
     1801 acgtatccac gctactagag gcataataag ctagactttc tttctctctg acattcatga
     1861 aaagtttaga gtgaggaaaa ccgccaaata aaccgttaaa gaccattagc gcaaaacgtt
     1921 ctggttcatc gtaatacaca tttgtctgat aagctaggtt cagctttgct tgaacgattg
     1981 gctcacgtac ttgtcgttcc ttcacgatat tcacttgagg ttgggtataa aacagatctg
     2041 gataaactgt ttcccgcgac tcaaatggta attgcccaac tgcttcagct gctttgtccg
     2101 gatcaacatc gccgacaaca aaaatgtcga tttgatcatt tgccatcatt tcttgatagt
     2161 aggacgctag cgaactagca gtgatcttgt ccaaatcttc gactacgccc aaccctggga
     2221 tcttttgttc cgggctgttt tcaaaataaa gttcttgcaa ggcataggaa gcaaaagttt
     2281 gtttatcttc cttcaaactt tccaaatata gacgcagatt gttcttttcc aagtcaaacg
     2341 tcaactgatc aaactgatga tttttaatat ttggataaaa taaaacctct tttaaaaaat
     2401 ctaccgcttg agaaaaaagg tttggatcat tgacatattc accattcaca aaactgatcc
     2461 ctgcgtttag ccaatgtaaa tttccttttt ttctaactga taatccaaag cttgcgccat
     2521 aaagctctgc aagttgcgca cttaactttg tctgatccga ataattcaaa ctatttgttt
     2581 ccaacatgct gctgagaagt gaacgtttcg tgatgacttc ttgttgcaga cgcgcagtaa
     2641 aacggataaa aatccggatc gttttgtatt gttttgttgg taagacatga agattcacgc
     2701 cttctgccaa ttgatacacc atatttatat gctccttttc acagttgatt gcccgcttac
     2761 tcttttaatg ggtcaacctg ccttttttac ttattcctct gcttgaaatt tataagttca
     2821 ggcaattcgc cgttttttct aaatgatgtt acactttata gtatagcata gatgtttttt
     2881 atcaaaagaa acagacggct aaaagcgtat attgtttttt tattttttat ttttcgatat
     2941 actaaagaca aataaaagaa gggagggtca ttcatgatca aagaattcaa agagttcatc
     3001 atgcgtggag atgtcttgga tcttgctgtc ggcgttgtta ttggtagtgc gtttacaagc
     3061 atcgtaaacc aagtggtgca aggcttgatc acaccactaa taggttggtt tgtcgcaatg
     3121 atcacaggta cttctgattt agaaggagca ctttcaattc ttgactggtc gccgacaaaa
     3181 ggcgtcacat ttgcgtttgg taacgttatt agtgctatca ttacatttct gatcactggt
     3241 tttgtcttat tcttagttgt caaagcggca aaccgtgcga agaatttacg ctccaaagaa
     3301 gagcaagtgg aagtggaaga aactcctact gcagaagatt atcttagtga catacgcgat
     3361 cttcttgcca aacaagtggc agaagacaat acacatccag tagagcaaac aaatctttcc
     3421 gatacttcta aataataaag aatctaaaaa aaagaaagcc gattttcagt gtcggctttc
     3481 tttttttaga ttcttaactt gctacttgtt cctttttcat gtcgatatgt ggaatatcat
     3541 cttccaaata cacatctgag attgccttga atccaaacga ctcataaaaa gcttgtaggt
     3601 attcttgtgc gccgatcacg attggttttt ctggaaaatt tttctggatc atgttaatcg
     3661 tttcttgaac aatctttctt cctaaaccat ctttacggtt gtcctgatga acaaggacac
     3721 gtccaaaatg gatactctcc gcttcttcat atactcgcgt gtaggcaagg atcttcccat
     3781 cagtatcttg aaaaaacgta tgccaagctt ctccatcaat gtcatcaact tcttgataag
     3841 gacactgttg ttccacaaca aaaactgcga ttcgttcttt gacgatcgcg taaaactctg
     3901 ctgttgacaa ttcttcaaat ttctttacct gatattccac taagcaaacg ccccctgctt
     3961 ttttattctt agcataccga ttcgcacaca aaaaagcaaa cgaaccttac gaatctcagg
     4021 ctcgtttgct ttttaaactg cttcagttaa aaattcgatc tcaactgtcc gagtaagtac
     4081 atctgaatag ctgtaagata ctcgttcaaa agaattttct tcaggatcca aatcaacaac
     4141 gaatactgaa ggataggttt ctgttaaaac gccgcggcgt tctgtttgac gctttcttcc
     4201 tgtctgagca accaacatga ttgggctgcc gatacgacct tctaaatctt tcttgattga
     4261 agctaaagtt gttggcatac agttcacctc tactatcaac tataccacat atttttaaaa
     4321 ttttcaagtt taccataaaa aaaaaagaaa tgcaataaaa ataaataaga agctcattaa
     4381 gatttttcta aattattcct cattataaaa tttcaagtgt ttttttattt tgagttttac
     4441 tctcccgtaa gcgctcatta cttgcttgtg atccattgct aaacattcag ccatgtctac
     4501 aaaatcaacg ccttgcatat aataatgaaa aattttagct tctatttttg aaaactggat
     4561 ttcgcaattt tcgattgctt cttttaataa aagatgctga attgctaaag gttcttgcaa
     4621 tgtgatctct gctaaaaagt ctggtccttc tgtctctatt ttttggtcga ttgagacttc
     4681 atctttttga tcacgacgct tcagcgcgtt ctgcttcctc aacagagaat agatttgatt
     4741 gcttataacg cttttataat aacttccaaa gctaacacct ttgctttttt cgtaagttct
     4801 caatgcctga tataaagcga tccttgcttc ttgttcccaa tcctcagaaa cataatttct
     4861 taaataatag gtgtttttca aacgtagtaa aagtggaaaa tatttttgat acagggcagc
     4921 aaatgcttct tcgtcaccat tacgtgcagc gcgcaataac tcctcagaca taaaataatt
     4981 cctccataaa ttcgtttatg gaggaatctt atcatcaatg attcatcgaa ctgattagaa
     5041 cgatatgtta tttatttttt ctcattaaat cgtccaattt ttgggacaat ttttttaact
     5101 gctcttcatt ccatggagaa ttacgtcgga aatcaataaa atgcatatct ttattttctt
     5161 gggctattgt tttttccgtt ttttttatcg tcttatataa ttcataagca gaagttcgta
     5221 atgctccttg ggaaaagatc atccactgtt ctgctaaatc gcttgtcgcc actgtcactt
     5281 gtgtcaaacg gtcattcagc tgaccagcga ttctctcgat ataactgtca gccgtttcgt
     5341 cttctttcgt aaaaacaaca tccaactggt atttcttata attttgttgg atgcccggaa
     5401 ctagttgggc atcgaagaca acgatgactt ccattccttc atatttcgca taatttgata
     5461 aacgatgtaa aagagcttct cttgcatccg ccattttgtc ctgcttttta agctggacaa
     5521 gttccggcca cgaaccaatc atgttgtagc cgtctacaat caacagctgt tttttcatgg
     5581 tctactcccc ctgacgttgg gaaaaagcct gatacataag caaaccagca gcaacactgg
     5641 catttaagct ctggacgtgc ccggtcatcg ggatcgtcag caattcatcc atttcttttt
     5701 tcaatcctgg actcattcct ttgccttcat taccaatgac taaagcaatt gatcctttgg
     5761 cattccattt ttggaagttt gtgccggtca tatctgtgcc aaatacccaa aatcctgttt
     5821 ctttgagctg ttgcacagct tgtaccaaat tcgtcacgcg tgcaacaggg acatgttcga
     5881 ctgccccagt ggaagtcttt gttacgatag gtgtgattcc aactgctcgg tgttttggta
     5941 tgatcacacc atcgacgcca cttgcgtctg ctgtccgtaa aatcgaccca aaattgtgtg
     6001 gatcttcgat gctatccaaa atcaaaaaga aggggtcttc ttttttctct tttgttcgtt
     6061 caatcagttg gtcaagggat aaatattcat aaggtgtaat cgcaagaacg actccttgat
     6121 gcacgccgtg atcgctcaaa gtttctaatt tttgtttagg aacccatttg actggcacag
     6181 cttgttcacg tgcgatttct ttcagttctt ctactttttc tcctcgtgaa tcttcttgaa
     6241 gaaagagttt attccctcgc ccttgttgta gcgcttctac tgcggcatgg tgcccaaaga
     6301 cgaaattatc ttctaatgct tcttcatttt gatcagaaac tgactgattt cgtccagttt
     6361 gctttttatt tgaatatcta ttttgttttt tgtcttcaaa tccacgtttc gattgatgtt
     6421 tgtttgttct ttctttaccc atttttttca ccaactttct gaatacacca gcggatcaat
     6481 tcttccatcc gctcggtttg ttttgtcaaa tgcagatagc ccatgagcgc ttcgaaccct
     6541 gttgcgaccc gatacgtcgt aacatcagca ttttttgcgc tcgtatggct tttagcattt
     6601 cttcctctgc gataataaag ttcttcttct tctgtcaaaa gatgttcttc cagcatttgc
     6661 tgaatcaaaa aggcttgtgc attcgcagaa acataattcg ttgccgcacg atgcagctta
     6721 ttgggttttg tttctccttg atcaatcagg tggtcacgaa catagatttc gtaaatcgca
     6781 tctccaacat aagccaaggc taaaccgttc aattgtgtgt aatctctcat gcttctcttc
     6841 tccatcgggt tccttgcggt gtatcttcta ataagatccc tttttctttc aacaaatcac
     6901 gaatttcgtc gcttcggaca aaatcacgat tgcttcttgc ttgatttcgt tcttcgatca
     6961 attgttcaat ctcttcatct aataactctt ctgctgtaaa taaaataccg aagatcgaaa
     7021 gccattgcga gaataaagct tctccttgtg ccaagacagc ggcagaaact ttcggctggt
     7081 ctagataagt gttcagccac ttagcaagct catagaccac tgtgatccca tttgctgcat
     7141 taaaatcatc gtccatttct tcgatgaatc gctgttctaa attttctaac aaagcgattt
     7201 tatccgcatc atctgccaaa ttctctgagg cagtttcttg gcggaatttc aaattgtcaa
     7261 acgtattctt cagtcgttgt aaattggtgg ctgcttcttt catcgtcgtt tcactgtagc
     7321 ggatcggacg acggtattgt gtggtcgcca tgaagaaacg caagacttgt ggatcaacgt
     7381 ttttgatcat ttcatgtaca gtgatgaaat tccctagaga tttactcatt ttttcatcgt
     7441 cttcgccaat cgttacatag ccattgtgca tccagtaatt agcgaatttt ttccctgttt
     7501 tcgcttcact ttgtgcgatt tcattttcat gatgaggaaa ttctaaatct tgcccgccac
     7561 catggatatc gatcgtttct cctaaatgtt ttgttgccat aacggaacat tcaatatgcc
     7621 aaccgggacg tcctttgccc catggtgaat cccaagaaat ctcgccaggt tttgcttgtt
     7681 tccataaagc gaaatctaat gggtcttctt tttgctgctg ttcttcccct gtacgctgac
     7741 ttgctccgac ttccagttca tcgatggatt ggtcacttaa tttcccataa ttttcaaatt
     7801 tacgggtacg gtaatacaca tcacctgaag attcataagc atagcctttt tcaaccaaca
     7861 cttgaataaa tgctaaaata tctggcatat gatccatgac tcttggatgc agtgtagcag
     7921 gttcgacatt caacgctttc gtatcttctt caaatgctcg gatgaaacga tcagcaactt
     7981 ctggtgctgt aatacctaat tcgtttgctg tacggatgat tttatcgtct acatccgtga
     8041 aattcgatac gaaatcgact ttatagcctc gatattcaaa atagcgtcga attgtatcga
     8101 aagcaatggt gctacgagca tttccgatat ggatataatt atacacagtc ggtccgcaga
     8161 catacatacc gacatgtccc ttttgtatag ggatgaattc ttcttttttt ctggtcaatg
     8221 tattgtagat ttttatcatt ctgatctcct cactttttct cctttgattc gaacgacttt
     8281 tgctggtact cctaccgctg tagcgtcgtc tggaacatct ttcaacacaa cggcacctgc
     8341 accgattttg gcgttttttc cgatcgttac cggtcctaat atctgcgcat tcgcagagag
     8401 catggctcct tgtttaacag ttgggtgtct tttacccgtt tcttttcctg tgccgcctaa
     8461 tgttacgcca tggaataaaa tcacatcgtc ctcgatttct gcggtttcgc caatgacgat
     8521 tcccattcca tggtcgatga acacgccagt tccaatcgtt gctcctggat ggatctcaac
     8581 accagttagg aaacgccaga attgagcatt gatcttcgcc aataaaaaga gccggtgctg
     8641 atataaaaaa tgagagaaac gatgccaaaa caaggcatga aaaccagggt atgtcaaaac
     8701 gatctcagct gtcgaacgtg cagccggatc gtttctcttt gctgcatcaa tcgctcgggt
     8761 aaaccaactc atgactttcc actcctttcg taagaatttt atgtaggaaa tcatcttctt
     8821 ataaagaaaa gaagcgcctt ttaaataagt ttccttatca aaagacgctt tccgcgtggt
     8881 tccacttttc ttcataatca atgattatct ctgacagcta acgcctgtta tacgttcttg
     8941 gctactttgt ttcctataaa acagtttttc ctgttttctg tgtttcgcca agaccactcc
     9001 cagaggcatt tcggcaacca ttcattgata ccttgcacca cccggtatct ctcttgtaat
     9061 gatcagctgc ttactttttt ctgttcatcg tgtttagttc agtacttgat ttaaatgtgc
     9121 taatgatttt tcacgaccaa gcaattcgat cgtttcaggc aattctggac catgcatttg
     9181 tcctgataca gctacacgga ttggcatgaa aagatttttt ccttttacgc cagtttcttt
     9241 ttgaacagct ttgatggcag ctttgacagt tggagcatct actacttcca tttgttctag
     9301 ctgtgctttg aatgcgtgga gaacagtagg aactgtttca ccagcaagta cttcttttgc
     9361 tgcttcattc aatacaggat gttcattgaa aaatagcgta gacagatcaa cgatttctgc
     9421 agcatagctc atttgaggtt gatacaggct aacgatcttc ttcacccatt cgattgtctt
     9481 ttcatctggg ttttctgcta cacgtccatc agcgattaga taaggcagac acatgtcagt
     9541 aaatgcatct agatccattg ctttgatgta atggttgttt acccattgca gttttttcgc
     9601 atcgaaagct gcgggtgatt tacttaggcg ttttggatcg aacattttga tcaattcttg
     9661 gtgttcgaag atttcgtctt cgcccacagg tgaccagccc aataatgcaa taaagttgaa
     9721 cattgcatct ggtagatagc ctaattcgcg atattgttcg ataaattgca agattgattc
     9781 gtcacgtttg cttagttttt tacctgtttc actgttgatg atcaatgtca tatgtccgaa
     9841 ggttggtggt gtccagccaa atgcttcata gatcatcagt tgttttggtg tattggcaat
     9901 atggtcatct ccacgtaaaa cgtgagtgat tttcatcaaa tgatcatcga ccgcaacagc
     9961 aaaattataa gttggcatac catcgcgttt ttggatgaca aagtcgccac cgatattatc
    10021 ggattcgaac gaaatagcgc ctttcaccat atctgtaaat gcgtattctg tattgcgagg
    10081 cacacggaaa cgtacaacag attctagacc ttgtgcttct ttttctgctt gttcttcagg
    10141 tgtcaagttc gcacatgttc ctgcgtaatg aggcatttct cctcttgcgc gttgtgcttc
    10201 ccgttctgct tctaattctt cttctgtgca atagcatttg tatgctcgat tgctagcaag
    10261 cagctgatca atcagcggtt gatagatttc tttacgttca gattgacggt aagggccgta
    10321 ttctccagga ttttcaggag attcatccca attcattccc aaccaagcaa ggttttccaa
    10381 ctggcttttt tcgccatctt cgatattacg tttttggtca gtatcctcaa tacgaataat
    10441 aaagtctcca tcattgtggc gggcaaacaa ataattgaat aatgcagttc ttgcatttcc
    10501 aatatgcagg tgcccagttg gacttggtgc atagcgtacg cgtactttcg tcattttctt
    10561 cctcttcttt caaactagac tttgactaac agcaaaaatt ccgtcagtta cggcttctca
    10621 aatatcattt atttaaagcc actcagcaca aattttacct tttctaaggt aaatagtaaa
    10681 gccaactggt tgaatttcct taccagttgg ctgaatttct tttttatttt gattgtttag
    10741 tctttttctc tgtctctttt ttattgtcga ttcctcgtcc tgcatgtgca ggtttagcaa
    10801 agatcatgcg tccagcagct gtttgaaggg cacttgttac aacgacttcg atacgttcat
    10861 tcatgtaatg ctgtccatct tctacgacaa ccatcgtgcc gtcatcaagg taggctactc
    10921 cttgttggcg ttctgtcccg gcttttacta ctagaacatt catcgtttca ccagggatga
    10981 cgactggttt tacggcattt gccaacgcat tgatattaag aacaggtaca ttttggaatt
    11041 cagagacttt attcaagtta tagtcattgg ttacaatgac accatctaaa agcttagcca
    11101 atttgatcaa tttgctgtct acttcactga tatcttcaaa gtctccatcg tacatttcga
    11161 cagatatacc ctcttctttt tgcaaggcat ttaggatatc taatccgcga cgaccacgca
    11221 cacgtttcaa actatcccct gagtccgcga tgtattgtag ttcgtaaaga acaaaattag
    11281 ggatcatcaa ggttccttct aagaatccag tctttgtaat atcataaatc cgaccgtcaa
    11341 tgatcacgct tgtatccaag attttatatt tatggaaatt ttctcctgat tttcgttcta
    11401 acagttgact ctcaacttga ctagctgttt cttgctcgat ttttttgctt cgtgaaccaa
    11461 aaactttctt ccattcttcg attcgtgtcg ttcccattct caatcccaga tagcctaaga
    11521 tgatcatcag taaaatcggc aatacactat tgacgaatgg aatttccata ttgtaaagag
    11581 gggtcgagat cacaacacct aaagtcaatc cgatgatcgc accgatcgca ccgaagagaa
    11641 gataagtcaa gctgacctca cttagttttt gttctacttg tttgataata gctgagatat
    11701 gtttggccaa cagcaaagat aataaaaaga aaataagtgc gcctaacagg ctgttggtaa
    11761 atgtattatt cagccatccg tttgactgtt gaccgattgc ctgccaagct gagggtaaaa
    11821 atgaaatacc caagcttgcg ccgacaatga tcatcagcaa agtaatgaca cgtttttgca
    11881 tcgtttttcc tccgtttcga caatcattta ttagttagcg gaacacacgc ttcaacgttt
    11941 cagccaaagt tgctactcca actatctcga ttccttttgg cgcttcccag ttacctaagt
    12001 tgtttttagg taggtatact tttgtgaagc cgagtttttg gacttctttg actcgttgtt
    12061 caatatgact gacacgtctg atttctccag tcagtccaat ttcaccgatg aaacattccg
    12121 ttggctgtgt tcctttttct ttatagcttg aagcaatact gacagcgata gctaaatcaa
    12181 tcgctggttc atttaatttt accccacctg cagcttttaa gtaagcatct tgattctgaa
    12241 gaagcaaccc agctcttttt tccagcactg ccatgatcag agatacccga ttgaaatcaa
    12301 gacctgttgt tgttcgtttg gcatttccga acatagtagg tgtgactaat gcttggatct
    12361 ctaccaaaat tggacgtgtc ccttccattg ccacaacgat agcagagcca gttgcaccgt
    12421 ctaggcgttc ttctaagaaa atctgtgaag gattagcgac ttcttccaat ccatgctcat
    12481 gcatttcaaa aatcccaatc tcattcgttg agccaaatcg gttttttact gctcgcaaaa
    12541 tccgaaaact atgatgttgt tcaccttcaa aatataacac cgtatccacc atatgttcta
    12601 acattcttgg gccggcgatc gagccttctt tggtaacatg accgacgata aaaatggcta
    12661 tcccatttgt tttagcggtt ttgagtagtt ctgcagtggt ttctcttact tggctgacac
    12721 ttcctgccac acttgtgatg tctggctgcg tcatcgtttg gatggaatca atgataacat
    12781 aatcagggga gatatgttcg atggcacgac tgatttcgtt catatctgtt tcagcgtata
    12841 gataaaattc tgtgttgata cttccgaggc gttccgcgcg taattttatc tgctctgcgc
    12901 tttcttcgcc ggatacatat aagacttttc cgcctatggc tgctaattgc tgagaaactt
    12961 gtaacagcaa agtggatttc ccgatacctg gatcgccacc aatcaagacc atagaccctg
    13021 gcaccacacc tccgcctaac acacggttca gttcttctaa ttttgttttg atccgaggtt
    13081 cttttttagg gacgacgtct gcgatttttg ttggttttgc tttttctcct gtcaaacttg
    13141 tccgagttct gcggtccgtt gtatcttgtt cgatttcttc gaccatcgta ttccattttc
    13201 cgcaatttgg gcagcgtccc aagaacttag gcgacacata cccgcaattt tggcaaacaa
    13261 attgtacttt tgattttttt gccattgtac cgcctccttt tatcctaatc atgagacatt
    13321 aaatcaagct taagtatctt taacgtttta tttactggaa gaaccaaatc cgccttgacg
    13381 ttgagcagtc gtttgatctt gatctgcttt taaaaacggc aaaaagatcc cttggccgat
    13441 acgctcgcct tttttgatca ctacatcctt gaaaccaaag ttggtgaatt ggaacatgat
    13501 atggccttca tttgcctcat tattgtaata atcactgtca atcacaccta ctccgtttgt
    13561 caacactaaa aaccgcttca acggattgct tgaacggtta gcaagctgca aaaattcatc
    13621 ttctcccatg taagctttga ttccagtagg cacgagggtt ggtttcaatt gtttttcatt
    13681 ttctgataca tcttttttca atccatctgc gactgtttcc aacccctttt tccagcctgc
    13741 tttccagata cttggaatca cgacctcttc tgctgcttca aaatcatagc cagcagcatg
    13801 atgtgtagcg cgctgcggta gattgatatt ttgatcttta tatttagaaa taacttcaaa
    13861 tcctcttttt ctcaccggtt ttcct
//
LOCUS       DBNICI010000069        13741 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.069, whole genome shotgun sequence.
ACCESSION   DBNICI010000069 DBNICI010000000
VERSION     DBNICI010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.069"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(5..661)
                     /locus_tag="AC1ZCC_001610"
     CDS             complement(5..661)
                     /locus_tag="AC1ZCC_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIQ1629868.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            854..1132
                     /locus_tag="AC1ZCC_001611"
     CDS             854..1132
                     /locus_tag="AC1ZCC_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629869.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1151..2359
                     /locus_tag="AC1ZCC_001612"
     CDS             1151..2359
                     /locus_tag="AC1ZCC_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIQ1629870.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2507..2872)
                     /locus_tag="AC1ZCC_001613"
     CDS             complement(2507..2872)
                     /locus_tag="AC1ZCC_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HIQ1629871.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3019..3792)
                     /locus_tag="AC1ZCC_001614"
     CDS             complement(3019..3792)
                     /locus_tag="AC1ZCC_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIQ1629872.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(3966..4826)
                     /locus_tag="AC1ZCC_001615"
     CDS             complement(3966..4826)
                     /locus_tag="AC1ZCC_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HIQ1629873.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4826..6244)
                     /locus_tag="AC1ZCC_001616"
     CDS             complement(4826..6244)
                     /locus_tag="AC1ZCC_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HIQ1629874.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6301..6750)
                     /locus_tag="AC1ZCC_001617"
     CDS             complement(6301..6750)
                     /locus_tag="AC1ZCC_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIQ1629875.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7195..7458)
                     /locus_tag="AC1ZCC_001618"
     CDS             complement(7195..7458)
                     /locus_tag="AC1ZCC_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HIQ1629876.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7557..8852)
                     /gene="rho"
                     /locus_tag="AC1ZCC_001619"
     CDS             complement(7557..8852)
                     /gene="rho"
                     /locus_tag="AC1ZCC_001619"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIQ1629877.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8849..10120)
                     /locus_tag="AC1ZCC_001620"
     CDS             complement(8849..10120)
                     /locus_tag="AC1ZCC_001620"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIQ1629878.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10285..11154)
                     /locus_tag="AC1ZCC_001621"
     CDS             complement(10285..11154)
                     /locus_tag="AC1ZCC_001621"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HIQ1629879.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            11257..11469
                     /locus_tag="AC1ZCC_001622"
     CDS             11257..11469
                     /locus_tag="AC1ZCC_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629880.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            complement(11446..13056)
                     /locus_tag="AC1ZCC_001623"
     CDS             complement(11446..13056)
                     /locus_tag="AC1ZCC_001623"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIQ1629881.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(13278..13523)
                     /locus_tag="AC1ZCC_001624"
     CDS             complement(13278..13523)
                     /locus_tag="AC1ZCC_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629882.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
BASE COUNT         3908 a         2893 c         2343 g         4597 t
ORIGIN      
        1 ttcatcaaaa gataatattg tcttctaaca agtccatatt caaaataacg atatgatggt
       61 ctttaacggt gataatacca tcttttttca gttgttttaa aattctgttt acactgctag
      121 cagttgagat gccacaaaat ttggcgattt cttcattcgt gatcgaaaaa tcgatcagca
      181 tcccttcttc tgtccgcaca ccgaataaat catataattc gtaaagttgc gtacaaacag
      241 ctcccagctt tccgtttaat agcatttgct gcattttttt catcgaataa agcagacgat
      301 agcggtaata ctcttttaca tagtgttgta gctttgggtc ttggttgata ttttgccaaa
      361 agcgcacacg attgattcga taaaactctg catattctga ttcaactcga atattatacg
      421 gcgaatcgat gaattgtgaa tattcgtctt ttaaaagaga gacgatttca agatctttga
      481 tatagcgaag gttaaattct ctcccatcat ttgaaataac acttgctttt accgttccct
      541 ctttcaagat atacgtatgg gaatcttcca tcccttcgaa tgtcaaatat ttatgatatc
      601 ctttgcgtat aatcggaaaa tcatttttat ccaaatattc ctttaagact ctactatcca
      661 atgatgctcc cccatccaaa ctttcatcta aaaagatcat acagtacttt aaaacttttt
      721 aaagtccaaa ggtattttac taatgatatt aacatttgtt aagtaccttt ttccatcact
      781 tatgcgattt ttttcctttt ttttattcgt tatactgcag aagtaaaatt ttttaagaat
      841 agagggaaaa aacatgaatt actggatgaa cacaatcatt aatcgattag aaacagcata
      901 tcaaacaaga tttgatatga aagcttcttt agtcttttta aatgatgcgt atcaaaactc
      961 gatcgaattg atcaaagcag tggatgagaa tccaacaaac gaatgcgaag aatttttaaa
     1021 tctgtttatg tctacaagag atttatttat tcgccaactt gttgatcgct atccatccaa
     1081 ttatcacgat gtagaagttc aaatacaaaa attaaaagca tattcagctt aaggggaatg
     1141 tataacatac atggaaaaga aattatttaa taaatatttt attggaatca cattgatcaa
     1201 ttttattgtc tacctgattt attatctgtt gatggtcatc atggcagtca ttgcgcaaga
     1261 caccttacac gccagcttga gccaagcagg ttttgcttct gggatctata tcatcggaac
     1321 attgctggca cgcttattca tggggaaaca gttagaacta tttggtcgga agtttacctt
     1381 aagaggcggc gcgatcttct atctcctttc aacattagca tatctttata cgccaactat
     1441 cggggcattg tatttagttc gattcttgaa tggattcggc tacggtacag tttctacagc
     1501 tacaaatgct atcgttactg catatatccc tgcatctaaa aaaggggaag gcatcaatta
     1561 ttatggatta agtactagtt tagctgccgc tgttgggcca tttttaggaa tgattttgct
     1621 gaatatgact gattttcgtt tcattatctg gttttcaatt gtactggtct tctttgttac
     1681 gattgcctgc cttgtctttc ctgtaaaaaa tattccatta tctgaagaac aaaaaatatc
     1741 tcttcgctca tggaatatca atagttttat tgaaaagaaa gcacttttca ttacaggaat
     1801 tgcctttttg atgggtcttt cttattcaag tgttttatca tttttatctt cttatacaaa
     1861 agtaatccac ttagttgctg ctgggtcatt tttcttcgtc gtatatgctt tggtcatcac
     1921 atttacacgt ccattgactg gaaggatttt cgatgtcaaa ggagaacaat acgtgatgta
     1981 tcctagctat ttattcttaa cagcaggatt atttttactg agtgtaacga cgaatagtgt
     2041 gacattgctt atctctggag cattagtcgg cctaggttat ggtacgttta tgtcaaatgg
     2101 acaggctgtt tgtttaaaaa tcgtccgcga acatcgaatc agtattgctt tatctaccta
     2161 ctttattggt ttagacctag gcttaggcgt tggtccttat ttgatgggga tgctaaaatc
     2221 tcttacttca tttagaggat tgtatgtgat tgccggagtc attccattga tatgtacagc
     2281 actctatctt gttaatggcc gaaaacatag tcatgaagca gagggcgaat tgattgaatc
     2341 attgaaaaat gaaatttgat cttgatgaat agataaaaaa aaccgttcag atctgaaaat
     2401 aatctgagcg gttttttgag taaaaatagt ttatgattat tcttaattaa acatctattt
     2461 atttttaaag ctttcattta tcgataagaa tagtttaact gttgatttat tccattttcc
     2521 gttcatctgg tacgatgcct tgacttagca accacttgtg gaagaaccat tctgccacac
     2581 cgatcaatag agcaggaatc aatactttca tccaaatatc gactgtatca ttcataaaat
     2641 atagatagag ccacaagata actgcagctg aaccagcatc tgcgatcgtc gctgcgatgt
     2701 tccctacttt acgtaagatc aacagatccc ctagcacata gatcatgact acaataatta
     2761 ccccaacaat cgttgaatcc ataaagctaa caccgtataa acctgtcagc acgacccaca
     2821 gaatcgccca gatcatgatt gccttcacta ccaacgcctt cacatgtttc attttctgca
     2881 ctcctttctc ctggataaaa aagtaagatt atctaacaag ataacctttt cattattata
     2941 ccatcggctg agagaagcga gcaaagataa ggtcctatct aaaaatgagg aagccactta
     3001 gaacattcac gtaataattt atccgtacag acattcgata tggtgttcct ttactttcgc
     3061 atactgttct tttgtcagtt ctttacttgt caccagataa tcgatttcat gaaaagcaat
     3121 ggcttgatgc atgaatttat gtgcaatttt ttgctgtgta actaaaacgg caagccggtc
     3181 tgattgtttt ctaaaagctt ttttgaccat tgcctctcct tcatgagttt ctgttactcc
     3241 ttggctgaaa tctagcccgc gacaactcat tatcgctaaa tccgcataat atgacaatgc
     3301 atcgttatag gcttttgaac cgttgattgt tgctcgtttc ggaacaattg cccctcccaa
     3361 tacagatact tttgccgttg tatattcact caacatagca gctgtgacga tgccattcgt
     3421 aatgatctgc aaaccatcaa atcttgctag gtttctgact aagtgtaaag aggttgtact
     3481 ggaatccaag aataagacca tatcatctcg aatcaaagaa gaagctgtat ctgcaatctt
     3541 acgtttttct gtttcttctt ctaatttggt gatcttcatc aggtaaggca cgtcaatttt
     3601 tcgattgtca ataagcatgg cgccaccgcg aactctacgg atcaatccac ttttttccag
     3661 aaaaacaagg tcacgtttaa tcgtggcttc gctataattt actaactcga tcaaatcggt
     3721 gactcttgca aattggttgt tttttagata attcacaatc tcctgctgtc tattctccac
     3781 ttcattcaac ataaaatcac cccatttact ttatatagta acaggaaaaa agaggttgtg
     3841 acagaagtga ttggcttcga gaaataagaa gaaatttttc gaaaaaacta cttctgaaca
     3901 tcgtgtgtgg ttaagagggt atgacaagac tattgtcata ccctcttgac cacacatttt
     3961 tatttttagt ataaactagc cttgcctact gtgttcaaaa tttccatttt atgtttgacc
     4021 acttcttgta cctttttatt agctgctgga taaattgcat tcggctcgta catcgatgga
     4081 ttttcactca atatttctct caaagcattg aaaaagacgc ttttaagatc tgagctcaaa
     4141 ttcactttgg caactccgta ttttactgct tcgctgactt ctgcgtccgg attacctgaa
     4201 ccgccatgta aaacaaatgg aagatcgatt tgttggttca attctttcag taagtcgata
     4261 tttaattttg gagttctgtt tttcggatac aagccatgcg ctgttccaat tgctacagca
     4321 agtgtatcga ttcctgtttc tttcacaaaa atcttcgctt gatcaggatc tgtataaatg
     4381 attgtttcag cacctccttc agcagaacca ttattcccaa tcgttccaag ctcagcctcc
     4441 actgagacat ttaccgcatg agctaacgca accacttttt tcgtaatctg tacattgtcg
     4501 tcgaatgatt tcaaagacgc atcgatcatc acggaagaat agccgtttcg aatcgcccgt
     4561 aagacatctt ctatcgtacc gccatgatcc aagtggataa cgatcggcag tttacttgta
     4621 taagcatagg ctcttactgc cgcaacaaac tcatcactta aataggcgat ttcatcagga
     4681 tgaatttcta agattactgg tgcttctaat ctctctgctt cttcgatgat tgctttcatc
     4741 atatcatagc tgcaaatatt gaaggctggt actgcaaatt gattctcttt agccactttc
     4801 aataactctt tcattgtata taacattacg cttccacctc atttgttaga ttttcatttt
     4861 tcgtccactt gtcttgtttt ttctctttca ctgttttttt caatagtcca agcataaagg
     4921 ctgtagtgac tgtacctgat gcaattgcca agacatacat aggtaaattg ttcataacat
     4981 ttggaattag catcacccaa atccctccat gaggtgccat tgaagtaact cctactgcca
     5041 tggaaatccc tgcagcgact gctgaacctg tcatcaaact aggtatcacg cggatcgggt
     5101 cagataccgc aaacggtata gcaccttctg taatatacga agcgcctaaa atccagcatg
     5161 attgacctga agtccgttct tctttggtaa atttatgttt gaataataaa gtggctaaag
     5221 caatacctag gggaggtgtc attcctgcag ccatacaagc agcaatcggt gcatttactc
     5281 cagctgacat caaaccgatc gagaaagtcg aagtcgattt attgatcggc ccacccatat
     5341 cagctgccat catgatcccg ataagtaatc cgaacaatag cccggatgta tttcccaagc
     5401 tattcagcca atttgtcaaa gcatccaaca cccatgtgat cggttgttca atcacaaata
     5461 ccatcaaaag cccaataatt aagatcgata aaaaggggac gacaatcagc tgataaatag
     5521 cagaaaattc tttttttacg ctaattttat ttgccagaaa atcacagata taccctgcta
     5581 gcacgccacc aatcattccg ccaagaaaac ctgaaccacc tgtagaggcc agtaatccgg
     5641 caacaattcc tggggcaaaa cctgcttttc cagcaatcga tgtagcaatc cccgcgccta
     5701 acgcaggaac catcatggca aacgctgtat ctcctccgat ccttgaaaat gcagctgcta
     5761 acacattata gtctgctgag ttgggatcag ccgctgtaat cccaaaggca aaactcagtg
     5821 cgatgatgat tccgcctgcg atcacaaatg gaagcatgta attcacacca ttcatgaaat
     5881 gattatagat tgtcggcttt ttctcatttg cagctggttc tttttcatta gacacaacga
     5941 atacgccaat atctctcaat gcttcgtcta ttactacttg cggttctttg attgctcggc
     6001 ttgtagagac acgctttact tttttcccgg caaatcggtc caattcgatt tctttatcta
     6061 ctgcaagaat gattgcatct gcctctttga tttcttgttc agataactga ttttctgttt
     6121 ttacaccttg tgtttcgaat tttgcttgat ggtttagatt tcttgctgtt acttctaatt
     6181 tttctgccga catataagta tgagcaattc cagtcgggca agcagttaca ccaacgattt
     6241 tcatgattat tttcctcctt tttcttcact atcgatactc ggacaaagac tctactattt
     6301 tcattcttga ctaatctgtc tgatgatatc tgctggcgta gtggcttctt ttattccctt
     6361 tctaaaatct gcattcatca atcgtctgga gagtgtgctg agaatctcta gatgcatatc
     6421 tgctgatttt tcaggaacta cgatggcaaa aatatattca attggcattt catctatccc
     6481 gctatagtcc gttaatttgg ctgtatgaaa aaaacacagt gctggttttg tcacatattt
     6541 gctttttgca tggggaatcc caaatccatc ttctattcct gtggagatct cctcctctcg
     6601 tttgaatagt gccttaacga ctttttgaac atttcttacc ttatttgttt ttttcaacac
     6661 atcccctatt cgctcaaata atgatccttt tgaatctatc gatggatcga aaacaatcag
     6721 ttcttctgcg atcaattgat caaacctcat aaaaaacctc tcccccacaa aaaatgttaa
     6781 cgttttcttg gaactgaggt cagtatagca tcgctagctg taccgttcaa tattatgaat
     6841 gaacagagga aaaagaccca tcatccgcgt gtataagtca ttaaaatgaa cgatctagct
     6901 cacaaacaat cgcaagtcta taacaaaaca aaaagccttc cttgaaagga aagcttagct
     6961 tctaatttta ggtaaatggt tttttacttc gaaataaccc ctcttcattt cgttaatata
     7021 aggtgttttt atcaagtgga taattaaaac tatatactag tttttcattg ttttgtgcat
     7081 tatgatccta cgactttttc agcatcaaac gaagttttaa attggcaaaa aacataaaaa
     7141 aacagactat ccctatcaga tagtctgcta aacgttgagt tatcagcgtt tttattattc
     7201 cgcagcgttt gcgtctttga gaccgtattt tttgttgaaa cggtccacac gtccgtctgc
     7261 ttgtgtgaat ttttgacgtc cagtgtagaa tggatgtgag tcagaagtta cttcgacacg
     7321 gatgactggg tatgtattac cgtcttccca ttcaactgtt tcttgtgaag tttttgttga
     7381 acctgacaag aatttgaaac cagttgttga atccatgaat actactgggt ggtaatctgg
     7441 atggatgttt tctttcatga ttttttcgct cctttgccct gctccatttg ctgaaccaga
     7501 gttgatttgt cgatttacaa cattaacatc ttaacatgtt tcagtcaagg ttgcaattat
     7561 cttttcagat ttctggtcgg atttttttta ccaaaggaga catcttgaaa ttctttgaaa
     7621 aattgctgat tgttttttgt tttacgcaaa aagcggatca gttgttccgt atattctaat
     7681 gaatctccag tcatattttt tcgaagtttc caaatttctt ctaattcttc acttggcatc
     7741 aataattctt cttttcgggt acctgatttt ttgatatcga tggctgggaa gatccgacgt
     7801 tcagataatt cacgtgaaag ttgtaattct agatttccag ttcctttgaa ttcttcatag
     7861 atcacgtcat ccatacggct tcctgtatcg actaaagcgg tcgctaaaat cgtcaaactg
     7921 ccaccttctt cgatgtttct tgctgccccg aaaaatttct taggtttata caatgcggct
     7981 gggtcaatcc cgccactcaa tgtacgtccg cttggtggaa cgactaagtt ataggcccga
     8041 gcaagtctag taatactatc catcaagata acgacatctc gtttatcttc gactaaacgc
     8101 attgcgcgtt ccaatactaa ttcagaaact cgagtatggt tttgcggctg cagatcaaat
     8161 gtagaagata ccacgtcgcc tttcacgctt cgttctagat ccgttacttc ttccggtcgt
     8221 tcatcaatca gtagaacgat taattctact tccggatagt tttcggtgat accatttgcg
     8281 atttctttta atacagaagt tttccctgct tttggcggtg cgacgatcaa tccacgctga
     8341 ccaaaaccga ttggagaaaa gatatcgatc attcgggtag atagtctttg cggtgttgtt
     8401 tccagttgaa gctgtctttg cggataaaga ggtgttaagg ctggaaaatg cggacgttct
     8461 tttgcttctt ccggatcttt tccattaacg ctttccacgt gcatcaaacc ataataacgt
     8521 tccgattctt taggtggacg ggcttttcct gccactttgt ccccatttct caaactgaat
     8581 cgtctgattt gtgaggcaga aatatagata tcttctgcac ttggtccata attgattggc
     8641 cgtaaaaaac cgtaaccatc ttgaccaacg atatccaata ttccttccat ataataaaat
     8701 ccttgtttct ctgcttgtgc acgaatcaca gctagagata attccttttt attcatctta
     8761 ctgtaataag gaattttaaa ttctttggca taactataaa cttcttttaa cgttttgttc
     8821 tctagttctg ccattgtcaa ataatcgctc attcagctcc cacttcctct atcatttcaa
     8881 ttgatgcccc taaggctgtt agtttctcga taatatgatc ataaccgcgt aagatataat
     8941 ctacattgta aatcgtcgtt gtcccttctg ccaatagtcc agcaatcact aaacaagcac
     9001 ctgcccgaag atcagaagcc acgacttctg tgccgtgaag tttatttgga ccattcacga
     9061 tgatcatatt tccttccacg gtaatatcag cgcccattcg tgctagttcc ggcacatggt
     9121 tcacacgttt cgcatagatc gtatcgatga tttctgaagt gcctgtagtc atcaatagta
     9181 acgcagtaag tggctgctgc agatctgtgg caaatcctgg ataaggatag gtcatgatgt
     9241 ttgcgggttt taattctttc gaggggtaaa cttcgatttc atcttcgctg attttcatat
     9301 ggacaccaat ttcctgcaat tttgctataa agctttccaa atgttcaaag atcacatttt
     9361 tcactttgat accatttcct actgcagctg ctaatgctag ataggttcct gcttcgatgc
     9421 ggtcaggaat catgaaatgc cgacagccat gtaatgtttc gactccttcg atacggatca
     9481 catctgtacc cgctcctcga acttttgccc ccatattatt cagtaaagtt gccacatcta
     9541 tgatttccgg ttctcgtgca gcgttttcaa ttactgtctg tccttttgct tttacagcag
     9601 ctaacatcac attgatcgtt gctccgactg aaaccacgtc catgaaaatc cgattgccac
     9661 gcaatccttt attttctgtc cgtaaataca tggcaccatg ttcatttgtc acttcagcac
     9721 ctagagcttc aaatcctttg acgtgtaggt caatcggtct agggcctaaa taacagccac
     9781 ctggcaggcc tactaccgct tcgccaaatt taccaagcag tgttcccata aaataataag
     9841 aagcacgcaa actattgatt tttccgtgcg gcatcggaat cgatacgact tctcgaggat
     9901 cgatttctag tatgttgttg gaaaaatgga cagttgcgcc catgatctct aaaatttcaa
     9961 ttaatgaatg aacatcttgt atgtccggca caccttccaa gatcactggg gaatcagcta
    10021 agattgcagc tggaatcaat gcgactgcac tattttttgc accgctgatc gttacttcac
    10081 cagctaaagg tcggtttcct ttgattacga tttttttcat ctgtttccac cttgtttatt
    10141 aatctttttt catggatatg cttgtatttt atcatagata aaaaaaagaa acgagcatgg
    10201 tctgccgttt taagtaaaaa agaatggaga gcgacttcaa aagtcgttct ccattcctat
    10261 atatcgtatc tgttatgtgg atgcttacgc tttgttagct gaaccgaacc agtcgatatg
    10321 agatttagca tcttcaacga ttgctgcttt acctggagct aataatttac gagggtcgaa
    10381 tcctttacct tctttatctt taccagcttc gatatattta cgagtagctg cagcaaatga
    10441 taattggaat tcagtgttaa catttacttt tgagataccc attgagatag ctttttcgat
    10501 ttgatcttga ggaatacctg atccgccatg taatactaaa ggcatgtctg gacctacagc
    10561 atcagcaatt gcttgtaagt ggtcaaaagc taatcctttc cagttttctg gatattgacc
    10621 atggatgtta ccgataccgc aagctaagta gtcgatacct gtagcaacca tttgtttgca
    10681 ttcttcaata tcagctaatt cgccgttacc gatgattccg tcttcttctc cgccgattga
    10741 accaacttca cattcaactg agataccttt tgcatgtgct ttttcaacaa cgtctttagc
    10801 taatttcaag ttttcttcaa atggtagatg tgaaccatcg aacattactg aagtatagcc
    10861 taattcgata cattccaaag cagcttcgta atcaccatgg tctaagtgga tagcaactgg
    10921 aacagtgatg ttcattgctt caactaaatc agtgattaaa tctttacaga ttttgtaacc
    10981 acccatgtat ttagctgcac ccattgatgt ttggataagt actggtgctt gttttgcttc
    11041 agccgcttca aggattgctt gagtccattc caagttgttt gtgttgaatc cgccgacagc
    11101 gtaaccgcct ttacgagcag cttttaaaaa ttcagctcct gatactactg gcataataaa
    11161 attcctccta agttttgtaa aactaaattt gttaagacaa ttcttaacca acgaccctat
    11221 tttaccaaac atttcggtag ttttctacta aaatcaatga aaatgaaaat tttcaagaag
    11281 ttttttgcaa aaaataaaat atccaatagc gctttaatcg aaaaaagtct tttgctttct
    11341 atcttttttc ctacaaataa taacaaatca gaaaaagaga aaagtgacaa gaaaattgct
    11401 tatccccttc tcttgctact tttctctttc tatcaagttc aaaaattatt ttgtttcgtg
    11461 atctgctaat gatgctccga caaatgcttt gattaatttt tgcgggcgat ttggacgaga
    11521 aatcaattct gggtggaatt ggcatcccac aaagaatttt ttctctggca gctctacgat
    11581 ttctactaaa cggttatctg gtgagacacc tgaaaagact agcccttgtt cttcaaacaa
    11641 ttgacgatat ttgttattga actcatagcg gtgacggtga cgttcttgca caacatcggc
    11701 tccatcatat gcttcagccg tcttcgttcc ttttttgatt ttacatgggt ataatccaag
    11761 gcgtaaagtt ccacccaaat tctcgatgtt ttcctgatca gccattaaat caataatatt
    11821 gtttgctgta tctggattcg tttcagctga tgacgcatct tctaatccaa cgacgttacg
    11881 tgcaaattcg acacaagcca tctgcatacc aagacaaata cctaaaaacg gaacgtcatt
    11941 ttcgcgagca taacgaatcg cttcgatttt cccttctact ccgcgatcac caaatccacc
    12001 aggaaccaag attccatctg cgtctttcaa acgttcttct acattttctc tcgttaattc
    12061 ttgtgctttg atccaatcga tctcgatgtc tgaattatag gcgaaaccag aatgtttcaa
    12121 tgcttcaact actgagatat atgcatctgg taactcgaca tatttaccga caagcgcaat
    12181 ccgtactttt ttcttcaagt tcaagacacg ttcttctaat tctttccatt ctgtcatgtc
    12241 tgcttctggt gtatctagtt ttaaatggtc gcaaacaatt tgatccattc cttgtgcttg
    12301 taaaactaat gggatggagt aaagtgtatc tacatctggc gactcgataa ctgcttctgg
    12361 ttcgacatca caaaattgtg ccaatttgtt tttcacgctt tgagaaacag gttgctctgt
    12421 acgtaccacc aaaatattcg gttggatacc taaaccacgt agctctttta cactgtgctg
    12481 cgttggttta gttttcattt ctccagcagc tttcaagtaa ggaatcaatg ttgtatggat
    12541 atacataaca ttatctgtac cgacatctgc tttcatttga cgaagtgctt caaggaatgg
    12601 caaagattct atatccccta ctgttccccc tacttctgta atgatcacat ctgaatcagt
    12661 cattttagct gctcgcatga ttttttcttt gatctcattt gtaatatgag gaataacttg
    12721 aacagttgct cccaagtatt cgcccttacg ttctttacgc aaaacttcag aatagatttt
    12781 tcctgtcgtc acgttggagt atttgttcaa attgatgtca ataaaacgtt cgtagtggcc
    12841 cagatccaaa tctgtttcag caccatcatc tgttacgaat acttctccgt gctgatatgg
    12901 gctcatcgtc cccggatcca cgttgatgta tggatcaaat ttttggatag ttacttttaa
    12961 gccgcggttt ttcaataagc gtcctaaaga agctgcaaca atcccttttc cgattgatga
    13021 aacgacaccg ccggtcacaa aaatatattt tgtcataagt tcaaaaactc ctttttaatt
    13081 tatgtgcagg aaaagtctca gacaataaac aaagctccct attcttcaat aagaataggg
    13141 agccgaaatt tctgtaaact tctgaccctc taaaagggtg cccaaggtgt ataatacaag
    13201 ctgactaagg gaaagtcaag tactcaaatg aatactatcc gcttacaatt ttttcttttt
    13261 cgatatcctt tgagaaatta tttcttaagt cctttatgag tgcaaaaaaa atttaaccca
    13321 cataataagc ttgatataaa catagctaac gttaaaccta agcaaatcag aaaatcgtta
    13381 ctgaaaaatt ttaagtgaaa taatagataa aaaaataaaa caaacattat gatagataat
    13441 atcccaccta acaaagaact actttttcct acttgcagca acagtttata tagcttatcg
    13501 tagaaacaat ctttttcttt cacccttatt tacctccttt acgtaaaaaa cgtagtccac
    13561 agttctcgga tttttggcat atcttttcta ttaaataata tcaaaaacaa aacaattcca
    13621 ctaatttttt caaagatgac ctttttttca acaaaagtgc cttgtaggga ttcattgaag
    13681 gtaacgaatc acataagtaa aaaataagga gatgcgacaa aagtcgtgcc tccttatttc
    13741 t
//
LOCUS       DBNICI010000070        13693 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.070, whole genome shotgun sequence.
ACCESSION   DBNICI010000070 DBNICI010000000
VERSION     DBNICI010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.070"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            312..482
                     /locus_tag="AC1ZCC_001625"
     CDS             312..482
                     /locus_tag="AC1ZCC_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629883.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            496..1098
                     /locus_tag="AC1ZCC_001626"
     CDS             496..1098
                     /locus_tag="AC1ZCC_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIQ1629884.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1114..1791
                     /locus_tag="AC1ZCC_001627"
                     /pseudo
     CDS             1114..1791
                     /locus_tag="AC1ZCC_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2102..2599
                     /locus_tag="AC1ZCC_001628"
     CDS             2102..2599
                     /locus_tag="AC1ZCC_001628"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629885.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDR
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            2600..3016
                     /locus_tag="AC1ZCC_001629"
     CDS             2600..3016
                     /locus_tag="AC1ZCC_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HIQ1629886.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3018..4580
                     /locus_tag="AC1ZCC_001630"
     CDS             3018..4580
                     /locus_tag="AC1ZCC_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIQ1629887.1"
                     /translation="MTEKNIMVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNS
                     SYEVQVSHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVIT
                     KSISRFARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQS
                     LSQNVKLGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQS
                     LVGIGRALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKN
                     EGHVPQYYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFC
                     GDCGDIYRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLA
                     GGNNMIKTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDE
                     LRDKRQILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHF
                     VIAFKSGIEMEI"
     gene            4723..4947
                     /locus_tag="AC1ZCC_001631"
     CDS             4723..4947
                     /locus_tag="AC1ZCC_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1629888.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            4962..5831
                     /locus_tag="AC1ZCC_001632"
     CDS             4962..5831
                     /locus_tag="AC1ZCC_001632"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIQ1629889.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            5812..6546
                     /locus_tag="AC1ZCC_001633"
     CDS             5812..6546
                     /locus_tag="AC1ZCC_001633"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIQ1629890.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            6579..7487
                     /locus_tag="AC1ZCC_001634"
     CDS             6579..7487
                     /locus_tag="AC1ZCC_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HIQ1629891.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7484..8026
                     /gene="sat4"
                     /locus_tag="AC1ZCC_001635"
     CDS             7484..8026
                     /gene="sat4"
                     /locus_tag="AC1ZCC_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HIQ1629892.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            8119..8913
                     /locus_tag="AC1ZCC_001636"
                     /note="aph(3')-IIIa"
     CDS             8119..8913
                     /locus_tag="AC1ZCC_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HIQ1629893.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            9189..9350
                     /locus_tag="AC1ZCC_001637"
                     /pseudo
     CDS             9189..9350
                     /locus_tag="AC1ZCC_001637"
                     /inference="COORDINATES: protein motif:HMM:NF019884.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            9396..9889
                     /locus_tag="AC1ZCC_001638"
                     /pseudo
     CDS             9396..9889
                     /locus_tag="AC1ZCC_001638"
                     /inference="COORDINATES: protein motif:HMM:NF024676.6"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            9879..9956
                     /locus_tag="AC1ZCC_001639"
                     /pseudo
     CDS             9879..9956
                     /locus_tag="AC1ZCC_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            10059..10955
                     /locus_tag="AC1ZCC_001640"
     CDS             10059..10955
                     /locus_tag="AC1ZCC_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIQ1629894.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
     gene            11054..11263
                     /locus_tag="AC1ZCC_001641"
     CDS             11054..11263
                     /locus_tag="AC1ZCC_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HIQ1629895.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            11280..11552
                     /locus_tag="AC1ZCC_001642"
     CDS             11280..11552
                     /locus_tag="AC1ZCC_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HIQ1629896.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            11554..12417
                     /locus_tag="AC1ZCC_001643"
     CDS             11554..12417
                     /locus_tag="AC1ZCC_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HIQ1629897.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(12680..13417)
                     /gene="erm(B)"
                     /locus_tag="AC1ZCC_001644"
     CDS             complement(12680..13417)
                     /gene="erm(B)"
                     /locus_tag="AC1ZCC_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HIQ1629898.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(13542..13625)
                     /locus_tag="AC1ZCC_001645"
     CDS             complement(13542..13625)
                     /locus_tag="AC1ZCC_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HIQ1629899.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
BASE COUNT         4853 a         2128 c         2848 g         3864 t
ORIGIN      
        1 tatgatacgt gtttgttttt ctgtgctgtt tagtgaatga ttagcagaaa tatagagagt
       61 aagattttaa ttaattatta gggggagaag gagagagtag cccgaaaact tttagttggc
      121 ttggactgaa cgaagtgagg gaaaggctac taaaacgtcg aggggcagtg agagcgaagc
      181 gaacacttga tcttttaagt tgctatcttt ttaaggtcaa tagagtatac ttatttgtcc
      241 taatatggtt ttagcagtat aattgacttg gtgaataggt catttaagtt gggcataata
      301 ggaggagtaa aatgaaaaaa tttatttatc gagttttaga aaatgacgaa gtggtggcta
      361 tttttaatga gcaacaatat gcgcaagatt ttatcgctta cgaaaagaca atttctgata
      421 agcaatttga aattgaaaaa gtagatatag ctgattggtt attgcaaccg agagaatttt
      481 agaggttggt tgaaaatggc taaaattggt tatgcacgtg tcagtagcaa agaacagaac
      541 ttagatagac aactgaaagc gttagagggc gtttctaagg tcttttcaga caaattaagc
      601 ggtcaatcgg tcgaacgccc acaattacaa gctatgctta actatattcg tgaaggggat
      661 atagttgttg ttactgaatt agatcgatta ggacgaaata ataaagaatt aacagaattg
      721 atgaatcaaa ttcaaattaa gggggcaacc ctggaagtct taaatttacc ctcaatgaat
      781 ggtattgaag atgaaaattt aagacggctg attaataatt tagtgattga attgtataag
      841 taccaagcgg aatctgaacg caaacgaatt aaagaacgcc aagcccaagg aattgaaatt
      901 gctaagaaaa aaggaaaatt caaagggcga caacctaaat tcaaagaaaa tgatccacgt
      961 ttacaacacg ctttcgattt atttttgaat ggttgttccg ataaagaagt tgaagaacaa
     1021 actggaatta atcgccgaac gtttagaagg tatcgatcaa gatacaacgt gacagtagat
     1081 caaagaaaaa aacaatgaaa agagggatag ttaatgagta cggttatttt agctgaaaaa
     1141 ccaagccagg cattagccta cgcaagtgct ttaaaacaaa gcaccaaaaa agacggttat
     1201 tttgagatca aagacccact atttacagat gaaacgttta tcacctttgg ttttgggcat
     1261 ttagtcgaat tagcagaacc aggtcattat aacgaaaagt ggcagaactg gaaacttgaa
     1321 tctttgccga tttttcctga tcgatacgat tttgaagttg caaaagataa gggaaagcag
     1381 tttaaaattg ttgctgaact tctcaaaaag gcaaatacaa ttattgtcgc aacagatagc
     1441 gacagagaag gtgaaaatat cgcctggtcg attatccata aagcaaatgc cttttctaaa
     1501 gataaaacgt ataaaagatt atggatcaat agcttagaaa aagatgtgat ccgaagcggt
     1561 tttcaaaatt tgcaaccagg aatgaattac tatccctttt atcaagaagc gcaaacacgc
     1621 caaattgccg attggttgat cggcatgaac gcaagccctt tatatacctt gaatttacaa
     1681 caaaaaggcg tacaaggtac attttcatta ggacgtgtac aaacaccgac tttatatctc
     1741 atttatcaac gccaggaagc aatagaaaac tttaaaaaag aacctttttt cctgaataat
     1801 tcatagtttt actaatagtc tttcctaaca ttgatttaat agtctttaaa gtattctgct
     1861 tcatcaccca ttaggtgatg aaaaaagaac ccctttctga tttagcggac aaatactttg
     1921 acctaatctc agaagatata aaagaaaaaa taataaaata tccgatttat actttgtata
     1981 aagaactaaa gtagttagca aatcatttac agcaaatcac tatgttccac ataccatttt
     2041 acatacttac cttatccacg acacatgttg agatagtatc gaagataaag aaagttgctc
     2101 catgttgttt ttgcgagatg gggaaatcaa agccacattg acaaccatga tgaataagct
     2161 tgctttcagt cataagctaa tcctagaacc acttttcaag tcagttagcc aaatcgatga
     2221 agaaagcgac cgtgaaagaa tggacgctat tgataagcta atggagcaac tattggaaga
     2281 acgcaatacc cttattgcac tgatgtccaa aggtttccta gaacctgctc tttttaatca
     2341 ggaacgaaat gttttagata gtgagattaa aaatcttact accgaaaaaa caaacctggt
     2401 aacgaactct gcgagtgggg ttttgcgagc aaacgagata aaggacctca ttaattatgt
     2461 gtcagcagat aattttaatg gtgactacac ggaagaacta tttgaagaat ttgtagtgaa
     2521 catcattgta aattccaggg atgagctgac attcaatttg aaatgcggtc tttccctgaa
     2581 agaaaaggtg gtgagataaa tggcatatat tccatatgga tacaaaattc aagatggagt
     2641 ggttactgtc gatgaaaagg cagcaggtca agtaaaggta ttctttgaga aatacatatc
     2701 aggactatcc cttacagtgg ctggcgaaca ggcaggtatt gaaaagacac attctgtgat
     2761 ggggcgcatt ttgaaaaacg tcaactacct tggaaatgat acgtatccag caattattga
     2821 taaagagata tttgataaag ctgaagaagt tagagataaa cgtgcaaagg atttaggacg
     2881 agtggtagag cttgccgctt tcacctctcc ccctcccaaa gaacgattca aaatgaaaaa
     2941 ggcagataat aagatgccag tggatccttt tgaacgagca gaatacttat acagtctgat
     3001 agaaagcgag gaataaagtg acagagaaaa atataatggt tattcctgct cgtaaaagag
     3061 taggaagtac agccgcaaaa gaaaagataa agaaacttcg tgttgctgcc tattgccgtg
     3121 tttctacaga aacagaagaa caaaattcta gctatgaggt tcaggtctca cactatacag
     3181 agtttataaa gaaaaataat gaatgggagt ttgctggcat ctttgcagat gatggtatct
     3241 ctggtacgaa cactaaaaag cgtgacgaat ttaatcgcat gattgcagag tgtatggacg
     3301 gtaacatcga catggttatt acaaaatcca tcagccgatt tgcgcgtaac accctagact
     3361 gccttcaata cattagacag ctcaaggata agaacatatc agtttatttt gagaaagaga
     3421 acatcaacac cacggatgcc aaaggtgagg ttttgctgac tattatggca tctttggcac
     3481 agcaggaaag ccagagcctt tcacaaaacg ttaagcttgg gctacagtac cgataccaac
     3541 aaggaaaggt gcaggtcaac cataagcgat ttatgggcta cacgaaagat gaagatggaa
     3601 atttaactat tgtccccgaa gaagctgaga ttatcaaacg catctaccga gaataccttg
     3661 agggtcagag tctagtgggc attggtcgag cccttgaaaa ggatggtatt ttaacagcag
     3721 cgggaaaacc aagatggcga ccagaatcag ttaagaagat acttcaaaac gaaaaataca
     3781 tcggagatgc ccttctgcaa aagactgtca cagtagattt tctgaccaag aaacgagtga
     3841 aaaatgaagg gcatgttccc caatattatg ttgaaaatag ccatgaagcg attattccta
     3901 aagagttatt tttgcaggcg caagaagaaa ttcatcggag aagcaatatc tacacaggag
     3961 aaggcaagaa caaaagaatt tacagtagca agtacgcttt aagtgccatc accttctgtg
     4021 gagattgtgg cgatatttat agacgggtct attggaatat ccacggaaga aaagaatttg
     4081 tctggcgatg cgtgactaga atcgagcaag gtcctgaagt ttgtaagaac cgaaccgtaa
     4141 aagaagatga actttatggt gcagtaatga ctgcgattaa taagctactt gcaggtggga
     4201 acaatatgat aaagacactg gaagagaata ttcatgctgt gattggcgaa accacagaat
     4261 accaaatttc agagattaac aactcactag aggaaaaaca aaaagaacta atcaagctgg
     4321 cgaacaaggg tcaagactat gaccacttag cagatgagat tgatgagctg agagacaagc
     4381 gacagatcct tttagtagaa gatgcctccc tcagtggcga gaacgagcga atcaatgagc
     4441 tgattgaatt tatccgcaag aacaaattcc gcaccttaga atatgatgat aagcttgtaa
     4501 ggaagataat ccagagcgtt acagtctatg aaggccactt cgtcatagcc ttcaaatctg
     4561 gcatcgaaat ggaaatatga aaaccagaag taaatagccc atgactctac agtagagttg
     4621 tgggttttct tttgcttgct gattataata atattttaat aactattgtg tttatcaacc
     4681 aaatggttta taatattctt agcgtaaact tggaggtgct gtatgactac ggcagaaatg
     4741 attaaagaac tgtgtgagca aatgaatata agtgtttccg aacttgctag acgtattggc
     4801 cagactccac agaatttcaa taaaaaatta caacgagaaa cagtaacctt ggatgagttg
     4861 aaggccattg ctgatgtgct aggtgtcaag tttgtgcagg catttatttt accagatgga
     4921 gaagaaataa aaatatctaa cgaataaagg aggatgttta tatggtaggt aatgttattc
     4981 aaatagtaac agagaaatta tcatctttgc cttttataga aggcatcgta ttagggggct
     5041 cacgtgcaag aggcacccat acagaaaatt ctgatataga tatcggaatc tattacaatt
     5101 cagactcatt tgacctgaca gcgattaatc aaattgctac agagttggat gatgagaata
     5161 gaaacaacct tgttgtacct cccggagcgt ggggtgattg ggttaatggc ggtggatggt
     5221 tagttataaa cgggtgtcat gttgacttga tattacgtga tataaaacgg gtggaacaaa
     5281 taatcaaaga tacggagcaa ggaattgtta ctgccaatta tcagaccggg catccccatg
     5341 gttatataag tgctatgtat cgtggagaat tagcgattag taaaatacta tatgctaaga
     5401 acgaaagctt atgcgaatta aaaaagcagg cagaaattta ccctactgct ctaaagaaaa
     5461 gcttgatgaa cttttttata tttgaagctg aattctcttt aatgtttgta aaagcaaatg
     5521 cgggggcaga ggataaatat tatattgcag gccatgtttt tcgtattatt tcatgcttaa
     5581 atcaagtact atttgcatgt aataatgctt attgcattaa cgaaaagaaa gctataaaac
     5641 tgcttgaaac ttttgaatat aaacctgaaa aatatgccga aagagttaat catatttttg
     5701 aagtactcgg tctttctctt tttgaatgct acgacatgac cgagaaactt tataaagaag
     5761 tgaatgaaat tgtatcggag ataaataact ttttaaacga ggagagttca gatgaaagaa
     5821 aacaaatatg atgataatat attttttcaa aaatacagtc aaatgagtcg ctcgcagaaa
     5881 ggactggctg gtgcgggaga atgggagact ttgaaaaaga tgctacctga ttttaagggt
     5941 aagcgtgtgc ttgatttagg atgcggctat ggatggcact gtatatatgc gatggaaaac
     6001 ggtgcttcct ctgtagtagg tgttgatatt tctcataaaa tgctcgaagt agcaaaagga
     6061 aaaacccatt ttccacagat tgaatatgaa tgctgtgcca tagaagatgt ggatttccca
     6121 gaggagagct ttgatgtaat actaagttcg cttgcgtttc attatgtagc agactatgag
     6181 aatttaataa aaaagatata taggatgctg aaggctggtg gcaatttagt ttttacagtt
     6241 gaacatcctg tttttactgc tcatggaaca caagactggt attataacga aaaaggagaa
     6301 atactgcatt tcccggtgga caattattat tatgagggca aacggacagc tatgtttttg
     6361 gaagaaaagg ttacaaaata tcatagaaca ctgaccacat atctaaatac actgctttca
     6421 aatagtttta taataaatca gattgtggag ccacagccgc cagagaacat gatggatatt
     6481 ccggggatgg cggatgaaat gcgacgccca atgatgctga ttgtatcggc aaaaaagaag
     6541 atgtaataat atagaaaaaa taaacgagga gtatgtaaat gagatcagaa aaagaaatga
     6601 tggatttagt actttcttta gcagaacagg atgaacgtat tcgaattgtg acccttgagg
     6661 ggtcacgcgc aaatattaat atacctaaag atgaatttca ggattatgat attacatatt
     6721 ttgtaagtga tatagaaccg tttatatcta atgatgactg gcttaatcaa tttgggaata
     6781 taataatgat gcaaaagccg gaggatatgg aattattccc acctgaagaa aagggatttt
     6841 cctatcttat gctatttgat gattacaata aaattgatct taccttattg cccttggaag
     6901 agttagataa ttacctaaag ggcgataaat taataaaggt tctaattgat aaagattgta
     6961 gaattaaaag ggacatagtt ccgactgata tagattatca tgtaagaaag ccaagcgcaa
     7021 gggagtatga tgattgctgc aatgaatttt ggaatgtaac accttatgtt attaaaggat
     7081 tgtgccgtaa ggaaatttta tttgctattg atcattttaa tcagattgtt cgccatgagc
     7141 tgctgagaat gatatcatgg aaggtcggca tcgaaacagg ctttaaatta agtgtaggca
     7201 agaactataa gtttattgaa aggtatatat ccgaggattt gtgggagaaa cttttgtcca
     7261 cctaccggat ggattcctat gaaaacatat gggaagcatt atttctatgc catcaattgt
     7321 tcagggcggt atccggtgag gtggcggaaa ggcttcatta tgcctatccg gagtatgata
     7381 ggaatataac aaaatatacc agggacatgt ataaaaaata cactggtaaa accggctgcc
     7441 tggatagcac atatgccgct gatatagaag agaggcggga acagtgatta cagaaatgaa
     7501 agcagggcac ctgaaagata tcgataaacc cagcgaacca tttgaggtga taggtaagat
     7561 tataccgagg tatgaaaacg agaattggac ctttacagaa ttactctatg aagcgccata
     7621 tttaaaaagc taccaagacg aagaggatga agaggatgag gaggcagatt gccttgaata
     7681 tattgacaat actgataaga taatatatct ttactaccaa gacgataaat gcgtcggaaa
     7741 agttaaactg cgaaaaaatt ggaaccggta cgcttatata gaagatatcg ccgtatgtaa
     7801 ggatttcagg gggcaaggca taggcagcgc gcttatcaat atatctatag aatgggcaaa
     7861 gcataaaaac ttgcatggac taatgcttga aacccaggac aataacctta tagcttgtaa
     7921 attctatcat aattgtggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt
     7981 tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg ttttagaatg caaggaacag
     8041 tgaattggag ttcgtcttgt tataattagc ttcttggggt atctttaaat actgtagaaa
     8101 agaggaagga aataataaat ggctaaaatg agaatatcac cggaattgaa aaaactgatc
     8161 gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt atataagctg
     8221 gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa agggaccacc
     8281 tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct gcctgttcca
     8341 aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag tgaggccgat
     8401 ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg
     8461 tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc ctatacgaat
     8521 agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct ggccgatgtg
     8581 gattgcgaaa actgggaaga agacactcca tttaaagatc cgcgcgagct gtatgatttt
     8641 ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct gggagacagc
     8701 aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag aagcggcagg
     8761 gcggacaagt ggtatgacat tgccttctgc gtccggtcga tcagggagga tatcggggaa
     8821 gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg ggagaaaata
     8881 aaatattata ttttactgga tgaattgttt tagtacctag atttagatgt ctaaaaagct
     8941 ttaactacaa gctttttaga catctaatct tttctgaagt acatccgcaa ctgtccatac
     9001 tctgatgttt tatatctttt ctaaaagttc gctagataga gttctatatt agagatgtaa
     9061 agagttttgg tgaagaggtt tgtgagtatg gcttttataa ccagggtgca atgagaagca
     9121 ctaacagcag ctagcttaag aacgcatagg atatactttt tatggaagtc acagaggagg
     9181 aataaaattt gaacaggatt aacagagtaa cctctattct tattcagctg caatcaaaaa
     9241 aaataattcc tgccaaagaa attgcacagc gatttaatat aagcttaagg acagtttaca
     9301 gagatatccg gacacttgaa gaagccggaa taccaattgg atctgaggcc ggaaaaggat
     9361 attttcttgt agagggcttc ctacttccgc cggtaatgtt tacagcggcg gaagtgggtg
     9421 cattgattac agcagggaaa tttttaaatt gccatggaga tgaatcattt ataaaggatt
     9481 ttgattcagc tatgtataaa atcaaatcta ttttgaagca tggtgaaaaa aactatgcac
     9541 aggagttgga gaacagtatt aacgtgtaca gcacatctgg acagaaaaat acattggcgg
     9601 ataacgttat tgcagcaatt cagactgcaa tctgcaataa aagggtaata tcaattcaat
     9661 atcctgcatc aggagggcaa gaaccggaaa gcagaatgat agagcctata tcactggggt
     9721 tttatgaaca gaactggtac ttaatcggtt ttgcaggcta agaaatgaat ataggaattt
     9781 tcgtgtggat cggattaaaa gcatttgcat agaagaagag ctctgtcaaa gccatgatgg
     9841 ctctttggaa caaatattaa aacaaatgct ttcatataaa aaactatatg ccctacaatg
     9901 ccgttgtaaa aattggggaa aagggatata ttgattttat atctttctca aaataaatat
     9961 aaaagccctt taaagagggc ttttatatat taatcacaaa tcacttatca caaatcacaa
    10021 gtgattaatc acaaatcact tgtgatttgt gattcttaat gatacaatat tactatacaa
    10081 aaaaagaatg gggcgtagtt atggagaagg aaaaactaaa aatacttgaa gaattaagac
    10141 gtattttaaa caataaaaat gaagcaatta ttatcttgaa taattacttt aaaggtggtg
    10201 ttggaaagtc caaattatcg actatgtttg cttacttgac agacaaattg aatttaaaag
    10261 ttttaatgat cgataaggac ttacaggcaa cattgacaaa agacttagca aaaacttttg
    10321 aggtagaatt gccacgtgtc aatttttatg aaggcttgaa aaatggaaac ttggcttctt
    10381 ctattattca tttgactgat aatttagact tgatccctgg cacgtttgat ttgatgttac
    10441 tgccaaaatt aactcgctca tggacgtttg aaaatgaaag tagattgctt gctactcttt
    10501 tagcaccttt aaaaagtgac tatgatctta ttattattga tactgtacca acgccaagcg
    10561 tttatacaaa taatgcaatc gtggcgagtg attacgttat gatcccttta caagcagaag
    10621 aagaaagtac aaacaacatt caaaactata tttcctattt gattgatttg caagaacagt
    10681 ttaaccctgg actagatatg attggttttg ttccttattt agttgatacg gacagcgcaa
    10741 cgataaaatc aaacctggaa gaactgtaca aagaacataa agaggataat ttggttttcc
    10801 gaaatattat caagcgaagt aataaagtaa gtacttggtc taaaaatggc attacagaac
    10861 acaaaggcta tgacaaaaaa gttttgtcta tgtatgagaa cgtatttttt gaaatgattg
    10921 agcgaatcat tcaattagaa aatgaaaaag aatagaatca caaatcacaa gtgattaatc
    10981 acaaatcact tgtgatttgt gataggtgat gataaaataa atagtaagaa gaaatagaaa
    11041 gaagtgagcg atcgtgggaa atttaggcgc acagaaagca aaacgaaatg atacgccaat
    11101 cagcgcaaaa aaagatataa tgggggataa gacggttcgt gttcgtgccg acttgcacca
    11161 tatcataaaa atcgaaacag caaagaatgg cggaaacgta aaagaagtta tggatcaagc
    11221 cttagaagaa tatatacgga aatatttacc tgacaaactt taaaaggagt gaaaatgaaa
    11281 tggcagttac gtatgaaaaa acatttgaaa ttgaaatcat taatgagtta tcggcaagtg
    11341 tttataatcg ggtattaaat tatgttttga atcatgaact agatactaaa aatactcgtt
    11401 tactagaagt gaatctttta aatcaattag aagtggcaca agaagttgat ttatttcaac
    11461 aaccatttga agaattacaa gctattcatg agtattggcg gtcaatgaat caatattcaa
    11521 aacaaatttt gaataaagag aaagtggctt aacatggcga atattactga cttcaccgaa
    11581 aagcaatttg aagatcgttt agaaaagaat gttgaacgac taactaaaaa tagactagcg
    11641 gttgaatcgc caaccgcttt tttacttggt gggcaaccag ggtcagggaa aaccagtttg
    11701 cgatcagcaa tttttgaaga aacacaaggg aatgttattg tcattgataa tgataccttt
    11761 aaacaacagc acccaaattt tgacgaatta gtgaaactct atgaaaaaga cgtagtaaaa
    11821 cacgttaccc cttattcgaa tcgcatgaca gaagcactta ttagccgttt aagcgatcaa
    11881 ggctataatt tagtgattga aggaacagga cgaacaacag acgttcccat tcaaacagcc
    11941 acaatgcttc aatctaaagg ttatgaaaca aaaatatacg tcatggcagt accgaaaatt
    12001 gaatcatact taggaacgat tgaacgatat gaaaccatgt atgcagatga tccaatgaca
    12061 gctagggcaa caccaaaaca agcgcatgat attgttgtca aaaacttacc gaccaattta
    12121 gaaacccttc ataaaacggg cttatttagc gatataaggc tttacaatag agaaggagtg
    12181 aaactctatt caagcttaga aacgccttcc attagtccga aagagacctt agaaagagaa
    12241 ttaaatcgta aagtatcagg gaaagaaatt caaccgactt tggagagaat agagcaaaaa
    12301 atggttcaaa atcaacacca agaaacccct gaatttaaag caattcaaca aaaaatggaa
    12361 agcttgcagc tacctacacc accaataccc aaaacaccta aacttccagg tatttaaacc
    12421 ttaaaaaagg aaaagagtag ttaccaaaaa acggtaacta ctcttttttt ataaaaacat
    12481 ttcctcatgt tggggtcccg agcgcctacg aggaatttgt atcgataaga aatagattta
    12541 aaaatttcgc tgtttttttc taatttcatt ttgaggtgaa ttaattggta gttattagag
    12601 gtgccctata cctaataatt tatctacatt ccctttagta acgtgtaact ttccaaattt
    12661 acaaaagcga ctcatagaat tatttcctcc cgttaaataa tagataacta ttaaaaatag
    12721 acaatacttg ctcataagta acggtactta aattgtttac tttggcgtgt ttcattgctt
    12781 gatgaaactg atttttagta aacagttgac gatattctcg attgacccat tttgaaacaa
    12841 agtacgtata tagcttccaa tatttatctg gaacatctgt ggtatggcgg gtaagtttta
    12901 ttaagacact gtttactttt ggtttaggat gaaagcattc cgctggcagc ttaagcaatt
    12961 gctgaatcga gacttgagtg tgcaagagca accctagtgt tcggtgaata tccaaggtac
    13021 gcttgtagaa tccttcttca acaatcagat agatgtcaga cgcatggctt tcaaaaacca
    13081 cttttttaat aatttgtgtg cttaaatggt aaggaatatt cccaacaatt ttatacctct
    13141 gtttgttagg gaattgaaac tgtagaatat cttggtgaat taaagtgaca cgagtgttca
    13201 gttttaattt ttctgacgat aagttgaata gatgactgtc taattcaata gacgttacct
    13261 gtttacttat tttagccagt ttcgtcgtta aatgcccttt acctgttcca atttcgtaaa
    13321 cggtatcggt ttcttttaaa ttcaattgtt ttattatttg gttgagtact ttttcactcg
    13381 ttaaaaagtt ttgagaatat tttatatttt tgttcatgta atcactcctg aagtgattac
    13441 atctataaat aaatacagaa gttaaacgat ttgtttgtaa ttttagttat ctgtttaaaa
    13501 agtcataaga ttagtcactg gtaggaatta atctaacgta tttatttatc tgcgtaatca
    13561 ctgtttttag tctgtttcaa aacggtagat gttttatcta cattacgcat ttggaatacc
    13621 aacatgacga atccctcctt cttaattaca aatttttagc atctaattta acttcaattc
    13681 ctattataca aaa
//
LOCUS       DBNICI010000071        13629 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.071, whole genome shotgun sequence.
ACCESSION   DBNICI010000071 DBNICI010000000
VERSION     DBNICI010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.071"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(1263..1928)
                     /locus_tag="AC1ZCC_001646"
     CDS             complement(1263..1928)
                     /locus_tag="AC1ZCC_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1629900.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2054..4090
                     /locus_tag="AC1ZCC_001647"
     CDS             2054..4090
                     /locus_tag="AC1ZCC_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HIQ1629901.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4094..4732
                     /locus_tag="AC1ZCC_001648"
     CDS             4094..4732
                     /locus_tag="AC1ZCC_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1629902.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4760..5188
                     /locus_tag="AC1ZCC_001649"
     CDS             4760..5188
                     /locus_tag="AC1ZCC_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629903.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5645..6556
                     /locus_tag="AC1ZCC_001650"
     CDS             5645..6556
                     /locus_tag="AC1ZCC_001650"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIQ1629904.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            6773..7210
                     /locus_tag="AC1ZCC_001651"
     CDS             6773..7210
                     /locus_tag="AC1ZCC_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629905.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            7305..8189
                     /locus_tag="AC1ZCC_001652"
     CDS             7305..8189
                     /locus_tag="AC1ZCC_001652"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIQ1629906.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            8413..11580
                     /locus_tag="AC1ZCC_001653"
     CDS             8413..11580
                     /locus_tag="AC1ZCC_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIQ1629907.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            11648..12145
                     /locus_tag="AC1ZCC_001654"
     CDS             11648..12145
                     /locus_tag="AC1ZCC_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HIQ1629908.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            12164..12628
                     /locus_tag="AC1ZCC_001655"
                     /pseudo
     CDS             12164..12628
                     /locus_tag="AC1ZCC_001655"
                     /inference="COORDINATES: protein motif:HMM:NF023225.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            12625..13383
                     /locus_tag="AC1ZCC_001656"
     CDS             12625..13383
                     /locus_tag="AC1ZCC_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HIQ1629909.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(13469..>13629)
                     /locus_tag="AC1ZCC_001657"
     CDS             complement(13469..>13629)
                     /locus_tag="AC1ZCC_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIQ1629910.1"
                     /translation="LKSYCINLSQLQKIDSAHYVLEFFNGDSLLVGRKIFEKTKERFH
                     DFQKTASS"
BASE COUNT         4642 a         2211 c         2749 g         4027 t
ORIGIN      
        1 aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt tgaccagatt
       61 ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg attaaatggt
      121 aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc tatatggaag
      181 ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag gttcctagcc
      241 tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt atcagctata
      301 taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg tagctctgct
      361 tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga agataagata
      421 actgtgttat ataaactagg taactaactg taacaagcta attttaagag attcttatta
      481 gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca tttcaataat
      541 gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg gaaaaaagtt
      601 cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca agagataaaa
      661 cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa tcccatagat
      721 taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa agtctcttcc
      781 tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa caatccattt
      841 tgtattttat tgagtactct tggataaact accagcgatt atcatattta tttatgttac
      901 tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa gaactaaaat
      961 tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact agattggtcg
     1021 ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct tgattatgat
     1081 tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc agaaatacct
     1141 atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa ttaatttgta
     1201 cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg ttcatctcct
     1261 acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata gtcttggctg
     1321 aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac gttcatgacg
     1381 tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc tccagcgtga
     1441 aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc gatggaattg
     1501 tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa aggattgaat
     1561 cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac caaacaaaag
     1621 acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga tgaataagat
     1681 ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt agctgattca
     1741 atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag catttcaagg
     1801 tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac accaattttt
     1861 tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat tttttctccg
     1921 gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac atgacctata
     1981 cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag aatcatttaa
     2041 gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa tcatagtgac
     2101 cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct atcggacaaa
     2161 tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac attttattgg
     2221 gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag aaactgagct
     2281 ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt ggaacttcaa
     2341 gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat cttctcacga
     2401 gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat cgatcaaaat
     2461 aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct atgaacggtt
     2521 catttcacta ttcactgatc gattcaatca agcatttcac actgattatg ccactaatat
     2581 ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag acgtatataa
     2641 ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat catccttttt
     2701 cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat tacatcaagt
     2761 attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg caggaatcat
     2821 tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc agatgtacgt
     2881 tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag ttgaaaaaat
     2941 cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat ttggattgtt
     3001 gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt tttcttcttc
     3061 acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact atcatgtatt
     3121 ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc gttttgcatc
     3181 agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat tagtcaatgt
     3241 atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa taaatctgaa
     3301 ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa taaccgaaaa
     3361 agaaacgcac cctgttatat tgatccctga acgatataga catacagatt taaaaaatga
     3421 cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga aatttctatt
     3481 tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga tcgaacatta
     3541 tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa atattctcag
     3601 cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag aaaggttatt
     3661 tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc cgtatactca
     3721 gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga tacagatatt
     3781 tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca tatattttgt
     3841 ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct ttgaaacgta
     3901 taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa tcttcctttt
     3961 tcttatagaa agagagccta aatatctttt taatattttt ttctttagct tcattgattt
     4021 tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat tgctgatgaa
     4081 tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa tcattcgaaa
     4141 caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg tgactgttgt
     4201 tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga atgatcagtt
     4261 cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg ggagagagaa
     4321 gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt tgattgaata
     4381 tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa atgtttcaga
     4441 taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga agctaagtac
     4501 gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga gagtcttatt
     4561 gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata aaagaaataa
     4621 ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag ttatcgtagt
     4681 cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt aaaaatcaaa
     4741 aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag ggataggttt
     4801 tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg gtgaaatcta
     4861 ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata ttattaagtc
     4921 aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct ctggaagatg
     4981 ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc actatagtcg
     5041 tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat cagcaaatgc
     5101 aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca cattctcatg
     5161 ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa tagaaaaaac
     5221 atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca tttttagttg
     5281 tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga acataaaagt
     5341 gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac atttgttcta
     5401 agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta acaaaagaag
     5461 tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa ctttctggat
     5521 tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg ttcttgatga
     5581 ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt aggagaattg
     5641 ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct tattgtcagg
     5701 ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa tcagcgaaca
     5761 agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc aacagttgat
     5821 cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag aaatcgattc
     5881 tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat tgaaatctgc
     5941 aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct ttcaagaagg
     6001 tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg aatctttcca
     6061 tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa aagatgtgaa
     6121 gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt caactgatac
     6181 tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa cggttccggc
     6241 agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg tgatcacttc
     6301 taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga aaaaccaaaa
     6361 caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa cagacacaaa
     6421 attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag acgctaatgt
     6481 aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag attctagtac
     6541 agcaagtagt aaataagcgg tataaataaa aaatatgaac tatttaaatc aataaaactg
     6601 ttcatctaaa tctttttatt agaactaaat caacagtagt ttaattacta cttgatttag
     6661 ttctttttat tttaaaaatt ttttttactt agaatccagc gaccctagat atttttctgt
     6721 ataatcttaa ataaagaaaa caatatcaaa aaatgcacgt agaagtagat aaatggagtg
     6781 gttttgcaaa tttcttttat ttctagaagt tattcacgta tacggatttg tttttctcac
     6841 aaataaaaag gcaggaagta caatggatca tttgtatata aaatatattc atcaattttc
     6901 cgttttggtg ttaggcacac taattatagc ggggattgtc gcatttatta ttcaaggccg
     6961 tgacatatta acagtacatc aagagttttt cagttatgca tttggtattc ctctgatcgc
     7021 tataaggtct gataacaaaa agtggaagaa acgctattac attctcttgc tatttttgtg
     7081 tttttatctg ccacctaaga tttttacttt attaggtata caatgcctat cattttcttt
     7141 tatttcctat atcattgttt taattttcct cagtttcata gtatggatgt tttatcttgt
     7201 caaaaaataa atgaatgttt catgaacagt acttcatcac agcctaattt ttttctttat
     7261 gctatcgtta tattaaagaa atcaatcaga agggaaagaa agtgatgaaa aaagttttat
     7321 tagtggtaaa ccccagttca ggcggtgaac aagcaaagga atttgagcaa ttagcgatag
     7381 caaaattgga atcggtgttt gatgaagtgg tcgtcttgca tacaaaaaaa gcaggggatg
     7441 caaaaaactt tactcgcgaa gcggctacgg aagggtatca tagtgtgttt gtcatgggcg
     7501 gagatggaac agtcaacgaa ggaatcagtg gaatcgctga acaagaacat cggccaaatt
     7561 tcggattttt cccattgggt acggtgaatg acttggctcg agcattaggg ataccgctag
     7621 aacctgagga agcgatcaat catttttcga ttgaatcagt caagccattg gatattggaa
     7681 aaataaatga cgactatttt atgaatgtcg tcgcaattgg ctctatccct gaatcaatca
     7741 atgatgtaga tgcggagaag aagacgaaat ttggaaaact agcttatttc atgtcaggaa
     7801 tcaaacagtt agcttccaca caatcctact cgtttcacgt agaagttgat ggaaagaagg
     7861 aagaaattga gagcagtacc ttgctgatag gactaactaa ctcagtcggc ggttttgaaa
     7921 cattacttcc aaatgcaaaa gtggatgatg gtaagctcca tttggtttat ttaaaagatt
     7981 cttctttgat cgatacgctt aaaacattgc cagacctatt aaaaggagta gatgaatcta
     8041 cgaaaaatct agcctacctt acttgtgagc aattagttgt gtcattagct gatgaggaag
     8101 aattgacgac gaatgttgat ggagatgaag gggataaatt acctgtgaca atcaaagtat
     8161 tgccctcaca tttacaggtt tattgttaaa atggaggttg tgaccgagga gttggcggga
     8221 gaacaccgct tgttcgggat aagggactgt gacaagactt ttgtcacagc cttttttttg
     8281 tacaaaaaag aaatttacac ctaatagaaa aatttttcca taaaaataca tattttaaat
     8341 atgaaagtac aattggaaat agattaaaac gataaaataa aattgtcaaa acgtcagaga
     8401 ggatggtgga gaatgaatca atgtgtaaaa aagctgattg cgtcgatttt gtttgtcttc
     8461 cctctcttga tggtgacatc cgtttttgcc gatgtagaga aaacaaacga atcaacgcaa
     8521 tcagagaatc aaagttttga caagactact ttttcagaag aatcgattac ttctgaaact
     8581 acagaatctc aaagtgaggc ttctactaat aaaaggcaag aagaaaataa gacaagtgag
     8641 acctcagaag tagctgaaca aacgtctgat agcagtgaag cggcactggc aagagctgtt
     8701 tcagcggatg ggtacagcga agctgccaca acagaagaac tggcgcagct tctcgcagac
     8761 gaatcagtaa ctaagatccg actgattcag cctttgactt tagacaggga attagagatc
     8821 aaacgagata tcgttattga ttttggtgga ttcgctcaca atttcggcac acaccatatt
     8881 tatatcaatg agatacctca gtcgatcgag ttccagaatt ttaaaggaac agctgcacat
     8941 ccaggtggat tgacaccaaa tatcgatgga aatgccatta tcattgctta tatgaccgat
     9001 ttttggggaa gcagatatca ttttacggga gaaataaaat ttaccgggac acttgatttg
     9061 tatgatgggt ccaaattagg actgatttac gctcctcgtg caactgtcac attagatggt
     9121 gtttccggtg tgttggatgt ccaaccagtg aaggaaggac tgaatgccgc tccgggtaaa
     9181 gcctattttt gtagaagcta tcaattgaat gtgatcaatg gtagtcaact ttatggaccg
     9241 tatctaggga aattctacgg ttttctagat tcaggagatg agactggtag tggaaaaaca
     9301 gcgccaggaa tccatatctt aagcggttca aaggtctctc ttgattatga tcgggcagat
     9361 ggcgttagcg atgaaggaga ggcagtcgat acgctacctg gtaatgtcat gtttgaagtc
     9421 agtggttcgg gaagcgaatt ttcggtaaat acaaagatca atattaccaa tgacaacaat
     9481 cgcggaatca tccaaatgag aggttcagga tcgaaagtca ctgtatcgaa tggtgggaaa
     9541 atcaccatca atacagcaac tacgggcgga tttcgcttgc aaggagattc tagtcaaatc
     9601 catgtatcaa gtggtggaca aatctcggtc aaaatggctg gagatgacga tcaacccggg
     9661 aacaatggga tgcgttttgt aggaaaaggc ctttctttga tcgtagaagg agccggatca
     9721 gttatcgaca tcgataagca gagtgggcgt tccagcgcag ttcgttttga gaatggtacg
     9781 caaaagctga cagtaaccaa tggaggctca ttgagggtaa gaaatacagg tgatggcaaa
     9841 tcttacgtga acagactcaa tcaaggaaat caagcgatcc aattttatga cgcttctggt
     9901 tttctagaaa gtccaggttc agctgatttt acagtcactg gagaaaaatc aaatatcgat
     9961 atacgcgcag attccggagc aactgtagat acaactggag atattgattt aaattttacg
    10021 gcaggtgaaa aaacttattt agtcatggtc gggaaaacag gggaagataa gaccggcatc
    10081 atcagtgggg atgtgttgaa tgtggactta gaatctccta cctattttga tttccaaaac
    10141 aaacggattg gaagcgagac caatggcata ggcggctggg tattccaagg aaatggagat
    10201 tcttctttaa agatcgccca ttcagaaatc gctttatggc gaaaaacagg cgcaacattc
    10261 gataactttg aaaaagaccc ggattatttt tctgatatga cagacttatc gatcaatggg
    10321 accgacttag gcaatttcgt atcaagccct agtgcaggtt taacaaatga atttgctcag
    10381 acaggtgccg gaatgaaaaa ttataatcga atcagtgcca ataatcagct cccgatgatt
    10441 gaatccttaa gagtgccaac gaatgcagat aaaaaaatct atgggcatgt cgttgttccc
    10501 gaaggtgtcg aggcaaagcc aagagatgct tggacaggcg aagtagaagt gactttacgt
    10561 attacttacg cagatacgac gaaatcatct gtagaaatta ctgctctcac gcaaggacaa
    10621 acagatgaaa atgaaggggc aggatataac ccatggggag aaggcgaaca aggcggcttg
    10681 ttcgaagttc aagtacccaa tgatgagaga ttaaaaacgg aggatcaagt gcaagttgtt
    10741 gctgctaaaa aagtaagagg tggccaagaa gtgacagaac tggactctgc caaaacgaca
    10801 gtcgatgtga ttcctcctga acctgctaat ttaagtacca cattgatcaa tgcagcttct
    10861 cgcacgatca caggtacagg tgaagcagga gccttggttt cattgaaaaa aaatcaagct
    10921 gttttgtcag aaacaactat tgatgataca ggggaatttg ttctttctat cccagaaaat
    10981 caactttttg caggagatgt attagaaatt gtattaaacg accaagcagg agaagctact
    11041 gctaagggtg tgcagaacaa gccaagtacc aacgatgaaa ttgggaatca caatccaagt
    11101 gatacgccag ttgtttatca tgatgccccg ccttttgcac ctgctacgaa gatcgtagta
    11161 gaaggtggtc tctcatttct tgccccttcc aaactcgatt ttggtcagat caaagcaact
    11221 ggtttgacac aagaagcatt tggccaaatg gatatacagg aaaaattatg gatttatgat
    11281 cagcgagaaa cgaaaaatcc gtggatctta acgataaaat tatctcagcc tttcacaaat
    11341 gatgcaggat tttctatgca ggatgtccta tatttcaaga aaggagagga gtatcaatta
    11401 gtgaatgaag aagaactgga aattgtcaga ggacaaaatt caacgaatgt gcaagcagat
    11461 tattcagaat atttaaataa cggtcgggcg tttaaacttg tcttaaccaa agaaaatcag
    11521 atagctggta attactcagc agagataaca tggacattag cagatgcgcc gacagaataa
    11581 agatcaagga ggatgattga tgaatcgaat atggaataaa agtcatcgaa acaactgctc
    11641 tttacttatg atccttctcc ttggattcat cctttggcag acacctgtcc atgcctccga
    11701 attcaatttt gcggtagatt ttgaattacc ggctaatcag attgataaga acaaaacata
    11761 tataaatgta aggatggagc ctgggaaaac acaggaaatc ccctacacgg tctttaaccg
    11821 aacagataaa tcagtaacag ttgagacatt gatccactcc gctacgacga attcaaacgg
    11881 tgtgatcgaa tatggagaat caaaaagtaa atttgatgaa acattaagtt atgatatgaa
    11941 aaacttagca aaaacagtgg gagaaataac tgttcctcct caaagtgcgg tcactgagac
    12001 actttcactg aaagcgccag aaaaagagct ggctggtgtg gtcgcaggag ggatcacgtt
    12061 tcaagaaaaa aaagagaacg tttctaacaa aagtacagtt ggtgtcgtca atcagttttc
    12121 tcaagtgaaa gcaatcttga tttgaaatag cgaaaaagag tcttccccag atttgaaatt
    12181 aaaaaaagct tatgcggatc aaagcgatgg aagaaatgtg attaaggcga atcttcaaaa
    12241 tccaaatgct tcttatatgt tcggcttgac tacatcggtt gaaatctaca aaaaagaaac
    12301 aaaagagcct tacctttcaa taaaaaaaga aaaaatagat gtagcaccca atacaaattt
    12361 tgatttactt gtcccattag aaggaaaaaa attagctccg ggacattatc aagcggttgt
    12421 gaagggtgct tggaaagaaa aacgctggaa tctccatacc gattttgaaa taactgatga
    12481 tgcagcggac caattgaatg aaaaggatat tgatttaaaa aatcagccag aaacatttga
    12541 ctggcgatgg ctgatacttg ttggagtcat tcttattggc ttatttgctg gattatttat
    12601 tttaaaagaa agaaggaaaa agaaatgaaa aaaacaattg cattaagtac cgttctagct
    12661 ggaagtctat tattatgtac acaaagtgtt tttgctgaga ataagagctc agccactaca
    12721 gaaggaacga tttctgtgac caaaccagat gctgcaaatc ctgaagacgt tgttccagtt
    12781 cctcctgttg atccaaatga tcctgatact ccttttacac ctgacgaacc aaatcaggga
    12841 acatccggat tactgacgat tgaccaagct ccagatcttg attttggaac aatcaagtta
    12901 ggtacagcaa aaacagtata tgctgcatta caaagcggaa cggacagtac tggagcacaa
    12961 acacaagtac agaatttcgt acaagtaacg gataaaagcg gaaattatgc cggttggaaa
    13021 ttatcagtca aacgaacaga atttattggt acaacatctg atacgaataa actaactggt
    13081 tcaaaggtga aattcaaaaa cgccgtgatt cgaaatagcg caactaatag cagtggaatg
    13141 ccatctaccg tttctggaac tggcgctgaa ggaatcgaaa tcccagcaga tcaggcagta
    13201 gatctagtaa ctgcaggagc aaatgaaggg attggcacat ggatttattc attaggagag
    13261 aatgtggaac aagggagaca atcagtggaa ttatttgttc ctgtatccaa ttatgctgta
    13321 gacaactacg tttcaacatt cacatggtcg ttaacagatg ctcctacaga agaggaagcg
    13381 taaaatcata tcaacgattt cagggagtgg agccgattca aggcccactc cctgaaatct
    13441 gctatgctta cgtctcagct cttattcatc aactcgaagc agttttttgg aagtcatgga
    13501 aacgttcctt tgtcttttca aagatctttc gaccaacgag taaggagtca ccgttgaaaa
    13561 attctaacac ataatgagca gaatcaattt tctgcaattg cgataagttg atacagtaag
    13621 attttaaaa
//
LOCUS       DBNICI010000072        13016 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.072, whole genome shotgun sequence.
ACCESSION   DBNICI010000072 DBNICI010000000
VERSION     DBNICI010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.072"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(1..344)
                     /locus_tag="AC1ZCC_001658"
                     /pseudo
     CDS             complement(1..344)
                     /locus_tag="AC1ZCC_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            417..593
                     /locus_tag="AC1ZCC_001659"
                     /pseudo
     CDS             417..593
                     /locus_tag="AC1ZCC_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(769..1338)
                     /locus_tag="AC1ZCC_001660"
     CDS             complement(769..1338)
                     /locus_tag="AC1ZCC_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIQ1629911.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1412..1735)
                     /locus_tag="AC1ZCC_001661"
     CDS             complement(1412..1735)
                     /locus_tag="AC1ZCC_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629912.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(1889..2701)
                     /locus_tag="AC1ZCC_001662"
     CDS             complement(1889..2701)
                     /locus_tag="AC1ZCC_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629913.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3028..3903
                     /locus_tag="AC1ZCC_001663"
     CDS             3028..3903
                     /locus_tag="AC1ZCC_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIQ1629914.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            3917..4240
                     /locus_tag="AC1ZCC_001664"
     CDS             3917..4240
                     /locus_tag="AC1ZCC_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629915.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4237..4989
                     /locus_tag="AC1ZCC_001665"
     CDS             4237..4989
                     /locus_tag="AC1ZCC_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIQ1629916.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            4979..7150
                     /locus_tag="AC1ZCC_001666"
                     /pseudo
     CDS             4979..7150
                     /locus_tag="AC1ZCC_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7262..9433)
                     /gene="copB"
                     /locus_tag="AC1ZCC_001667"
     CDS             complement(7262..9433)
                     /gene="copB"
                     /locus_tag="AC1ZCC_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HIQ1629917.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9453..11639)
                     /locus_tag="AC1ZCC_001668"
     CDS             complement(9453..11639)
                     /locus_tag="AC1ZCC_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIQ1629918.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(11639..11848)
                     /gene="copZ"
                     /locus_tag="AC1ZCC_001669"
     CDS             complement(11639..11848)
                     /gene="copZ"
                     /locus_tag="AC1ZCC_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HIQ1629919.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11861..12301)
                     /locus_tag="AC1ZCC_001670"
     CDS             complement(11861..12301)
                     /locus_tag="AC1ZCC_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HIQ1629920.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12375..12914)
                     /locus_tag="AC1ZCC_001671"
     CDS             complement(12375..12914)
                     /locus_tag="AC1ZCC_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIQ1629921.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3660 a         2733 c         2315 g         4308 t
ORIGIN      
        1 gtagaatttg cagcacctgc tcgagttgga tcaattactg gtttatcctc cgaagtagtt
       61 ggattatcat ccgtaaaacc atgagccaaa tcataggcta gtttttcttt acttacaccc
      121 atttcagaaa gataaccgta aggatctgta tgatcgcccc aaatattttg tgttacccat
      181 aaatgcgatt tgattcctgg ttggttataa ggagtgtcta atgttaatgg aataccatat
      241 ttcattgctg aatctcttgc caattcaacg tatactttat agttcttttc aaaagtttct
      301 ttatcatgtg tatgttgtaa ctcgatttgc acaggacttt tggctcacga ggttgtccta
      361 ctctacatct tgctgataga tatgctgaaa ggaaaacacc aacattatgt tttgcttttt
      421 cagctagaac gagacaagca ggaatacctt tagaagatat caaaggtttt ttaggtcata
      481 aagatgtttc cactacacaa gtctatgccc atatttctcc cgaagtgaaa aaaagatcaa
      541 tgaatcaact agaaaactat atagaagagc aaataaaaaa gcactcaaat tgagtgcttt
      601 tttaagctca ctatcactaa aactatcact aattttattt ataagcgaac atgtttctat
      661 cactgatttt ttaaatctag cgaaaaaccc ttatcttaaa ggcgttcgtt caattctttg
      721 gcaaggtctt caaagcctgg cttgcctaga agtgcgaaca tggttttctt atagctcatg
      781 tagaaaaatt ttcttgatga tgattttatc catcaattga gattgactgt tttgctctcc
      841 tatgatttca gctatgattg tccgattttg cggttttctt cttaactggt agttgatttt
      901 cccagtttcc atcaactcgc taagcatatc cacgactccg ccaaatccgg tctttttcgt
      961 aataaaacct agtgctacca ttcgttcatc tagatttttt gctgtaatcg tcacaccagt
     1021 aatgagcgag tgctgtaatg tttgggccag ctcttccatc ccttcagcat tggatacgac
     1081 ggaagctgtt ttgttaatag gttcttcgtt tatttctttt ggttctttcc tctccacttt
     1141 cactgtttga cgaaggacat ctagttcttg caagagcgtt tcgtattgtt cttgggtcag
     1201 catcacgcca gctacttttt cacgattaaa tatatatacg ccagttcccg cgtctcgcgc
     1261 ttgagcaaat acatccatcg gcgatcgttt cacttctgtg atcgaagatg tgggaacttc
     1321 taatttcttt aattccaatg gatttcctcc tttactaatc gctttttatt atagcacaga
     1381 ctgacttgtt ttgatctttt ttccaaaggt tttatcggtt tttgcctaac cagtaattga
     1441 tatcggtaag aagtctttcg ataccaaatg aaggatggta cagatacgtt ttttgatctt
     1501 cgttatggtc tagtggaaaa aaatgatcga tgatcactag atctgcatac cgaggcggct
     1561 tatttcgagt acatacatgt atagagatcc gttttcgatg tcttagcgtt ttgttcagta
     1621 ccccagcaag tcttcttgct tcttggtgtt catagcgaaa aaaaaagacg attttcactt
     1681 caggagggtg gacatctaaa taccctcgtt cctggagtgt tttcagtaaa agcatccccc
     1741 actctactgc ggggtgtgca agtgcttgtt ggatcgctag ccgaaattga tatagtttat
     1801 ctttggaatt gggaaatttt tctaatgttt cctgataata acgaacttct tctatcagcc
     1861 ggtcatggat accaattacg taacgaagtc attcttggta aagtactttt gtcagctttg
     1921 atttgctttc gctctcaaat tcgctattca gccaaaacag cgaatcaagc ttttcagtca
     1981 caatcgtcaa tttccgatag tcttgtgtct cttctatccg tttccaccgc ttttcagtaa
     2041 tctggttaca gctcaagcgc aaaattgcta gataattttt ttcttttatt ggcaaaatct
     2101 gctcctctct aaatagcaat tcaggaaaca attcaaagaa tagtgttcga ctgtctccta
     2161 aaatctcgat ttctgccgga tcctttcttt gcttcaataa ttggatgaac atctcagaaa
     2221 aggactgctc tccatcatct gcaaaaaaat gcggaaaatc tttcaaaaag caaaacagca
     2281 gatatcttac ttttctttct gaaccaaaga tctgtgcttt tttataagaa aaagaaaggt
     2341 ggtactcgcc aaaccacttt ctcaatcgct cttgcgtaga acgatcgtac ttctgctcgt
     2401 aaggttcatt caatagcaga tttttcagta aacaaaacgg gggtgattgg atcagaaaaa
     2461 caaacaagag ataaggttcg ctatatcctt gatcataaac taactgaacg gttttcttat
     2521 gaagaatcag ctgaatggga cagtcgcttt ccgccaaacg tttcagatgc cgatccactg
     2581 ttttaacatc gattttcagt tcttcagcta aatcagcttt tcgccagatc aaatctttca
     2641 ttagattgat tagtgaaaat aaattctgtg cattttttga taatagtttt tgccatctca
     2701 tcgatcattt ctcacctgat ttctaaacat attgtttgat caaaatgggt tcactcctaa
     2761 aaagataaaa aactcccttt ctatttattt aaatacgcaa atcttcctca ttatttttta
     2821 ttttactcaa tttaatcgta ttctcatttc aaaacgaatg tactttaaaa aaacataata
     2881 agatcacttt gcttgtagga tgtattccct tccattacaa tgaaataaat agcgatttta
     2941 tgagaaggtg atgtgaaaat ggaagaaaaa atagaagaat ccaaacaaaa aatcgatcag
     3001 ttgatataag aattagaaaa agcggtcatc ggcaaacgtt caactatcca attgatgatc
     3061 gtggccctat tagcaggtgg tcatgtctta ttcgaagata ttcccggtgt aggtaaaacg
     3121 ttactggtca aagcactagc aaaagctgta cagggaacct tcagcagagt ccaatgtact
     3181 cctgatctgc tgcctagtga tattcttggt ttttccgtat acaattcaca atcaaaagag
     3241 tttgaatttc gtccgggacc gatctttacc acgatcttgt tagcagatga gatcaatcgt
     3301 acgactcctc gtactcagtc agcactttta gaagcaatgg ccgagaatca tgcaacgatc
     3361 gacaacaata cataccctct agacaatcat ttttttgtct tagctacgca aaatccaatc
     3421 gagtacgaag gaacatatcc tttgccggaa gcacagctcg atcgtttttt attccgacta
     3481 caaatcggtt atccttcttt tgatgacgaa cttttgctac ttttggataa attcgaaaaa
     3541 gtgttggata acttaaatga agtattgaac agttatgaaa tagaagaaat aaaaacaaat
     3601 gtggcgaatg tctttgtcag tcccgaagtg gcatcttatg ccttgcagtt agtgtcagca
     3661 acaagaagtc atgctgccat ccagcttggc atcagtccac gaggaagcct ttcctttatt
     3721 caggcagcaa aagcttatgc tttaatccat gggagaaatt acgtcacgcc caaagatttg
     3781 caggatttag ttccttacgt gttttcccat cgcctgattt tttacgatcg ttcattgaag
     3841 gaagacgaaa agaaacaaat cattcatact cttgttgcac aaattcctat tcctgtgagg
     3901 tagattgagg tagacaatgg aaaaaatcaa agattatttc attaattttg tgttatttct
     3961 ggtatatttc atcattgtgc tttttgctct tgtattcaac aattctttag gctggttctt
     4021 gttggccttt tttactcttt atgtatcatg gagcttgatt tcattaagca gctttctaaa
     4081 gaaactttcg gttacttgta cattgcagcc actcgtttat cgcaaaaaaa gtatgcaggt
     4141 tattttttcc atagcaaaaa agagaacttt ttctttacct ctccctcgtt tacaaatcgg
     4201 cttcccagca atctttgcag aaaaaaacaa gagattttga ttcttacaaa taaaccgcaa
     4261 gaagtccaat tgctttggca gccaaaagaa agaggatgcc atgaaacatt aactgtcatt
     4321 tttacagcct atgatagtct aggacttttc agaaaaagtg caaaaagaga gatctctttt
     4381 cctgtgactg ttctgcctgc tttttgtaag attcaagcag aaaaattaca gagagttctg
     4441 gaaaaaaaac agcagctgaa cgcgtaccaa agaggtcttg attttcggga acatcgcgct
     4501 tatcgtccag gtgattcttt caaccgaatc gattggaaat tgtctgctca tactcaagag
     4561 tggttatacc gtgaatacga ttatcaagaa gaagaatgtt ctcctctttt ttgcttttgg
     4621 ggttcgccca gtccaaaatt tgaacctctg ctagacttgt atttttcttt atggcatttg
     4681 agaaaagaaa agcagccgga acttctgata ttaggcaacc gcgcctttca tggcaaagac
     4741 cccggtcata cctattttgc ttctttggaa gctggcgatg agaaagcttt tttcgcacac
     4801 ttgaaaaaat atgcaaaaaa acaaatcgtc ctctttgttc ctgagaattc tcctgaagtc
     4861 tcagaagcta ttgaaacatt gtcaaaagat cgaacggttt atcttgttta tttcgtagaa
     4921 aaacaactca tggtccaaga aaaagacaag atatgttcat tgccaggagg tgaatggatt
     4981 gacgattaaa cgggaaaaat ggctgcgtgg cgtttactcc tttgtgatac taagtgtagg
     5041 tgctccgata tttgtagtag ctaatcaatt gcaaaacagc taccttctta tcctaactat
     5101 tggtgtccta tgccttattg tctgctgtat tcctaaaaaa tgggtacgtt tgctgctttc
     5161 tttgccagta atgataggtt gtttgttttt atattttcct tcgaagacat tttccatatt
     5221 atggatctta cagttttttt cgtcagtcac agaggaattc ttccatctat cccaaggtaa
     5281 actttattat ttgccagaaa agacagcttt ttttctcata atgttgctga tctatttatt
     5341 tattacattg accgtggatt acgactattg gtacgcaccc tttctttctt tgatggctta
     5401 cttcatgatg cttaccgttt ttcggaaaaa agatttactg tacgaaatga ttgcggtagt
     5461 aactgttttg ttcgtgtatc tggcagcaag gcaatttttc tccatccggt tgttgtcggg
     5521 aaacactcga ttccagtcat tgactacagt tctgcttatt tgtttcttaa cttttagcgc
     5581 tatcttgcct ttatatcttc cgaaagtaca ccaatctctc gaggaaacga gtgaaccgat
     5641 ccgagaatat cttaatgatg aagggcttta tgacacactc catgagtatc aattaaccgg
     5701 aagtgcaaga acaggcttca gtgagaatga tgaacagctt ggtggacctt tatatgataa
     5761 tggcgaagtt gtttttacag cgaaccaaaa aggaaatggc tattggaaga tcgaaaataa
     5821 aaatcattac accggttctg gatggataga agatcaggac gcacccatcc aaccaatcca
     5881 agaattccct gttgacatca cgagctacca aaccgttata gaagacaaag aaaacattac
     5941 attgacccta aaaaaacaag ttttcttacc ttatccttat ggaaatacta ccttgactga
     6001 tttttcatta gctagtggac aaacgcttta ctatttgcct gaaagccagc gattcattcc
     6061 tgtcccgaat gaatcgacac gttctgcaac tttacaaaca gaaaggttag tttttacgag
     6121 agaagaatta aaaagcagtc agttaaaagc tgatgattta gaaaatcagc agacaaatct
     6181 tcaattgcca aaaagtttgc ctaaacgtat tgaggacttg gcggtcgaat tgaccaaaaa
     6241 tgaagcaact ttaattcgta aagtcgaagc tgtctaacag tatttaaaaa caagcggaaa
     6301 tttccgctat tccaaaacgg ataccgggca tactccaaaa gaacacgatt atgtggatta
     6361 tttcctcttt gattcaaaag tcggctattg tgacaacttt tcaacttcta tggtcgtcct
     6421 tcttcgaaca cttggtatcc cagctcgctg gacaaaaggt tttacaaatg ggctcagaac
     6481 aggcacagat aatcaaggga atgaggaata cagcattctc aatagtcatg ctcattcttg
     6541 gccagaggta tactttcctg gatttggctg gcttccattt gaaccaaccc cttcatttgc
     6601 caatccaatg ggttcacaac cagttgctga aacaaccgaa accgtccagc ctgctatcga
     6661 acaaccaaat gaaagcgaaa atatggcaga aactatttcc agtgagagat ctgaggtcat
     6721 tccatcagaa gaaacaacac tcacaccagc ttataaaatc gtcttattca gccttggagt
     6781 gtgtttatcc gtcttgttgt tctttctatt gttattcaga aaatctctgc gctggacaag
     6841 ttatcttatc tatcttcatt tttttgcttc tactaatacg attgcatatc aaaaacttct
     6901 taaccctttg gaaacattgg taccgcgcaa acccgcagaa tctttgcagg aatatagcct
     6961 gcgaatagaa caagaattca agctagatct gcgtttcatt cgattaacta ctcaatatga
     7021 agcagaaata tatggacgac aagcagatcc ggtaaaattc gataaaaaaa cctatttaga
     7081 aataacgcat ataataagaa aaatggtata caaaaaaatt ctgacaaaag ggagccgctt
     7141 caaaaaataa gataaaattt ctggagaatc tacttctgga atgccgagaa ccagctgatt
     7201 gagtatcgat tactcgaaaa aaaggttgtg acacgttttt tgtcacaacc ttttttcatt
     7261 attatctgat tttcaatgtc aaagcattta gagctacaac taccgtactt aacgacatca
     7321 agacagcacc aactgctgga cttaatatga taccaattgg cgcaagaatt ccggctgcta
     7381 gcggaattgc aagaatattg taacctgccc cccaccagag gttctggatc attttctttc
     7441 gcgtttgctt ggctagatca agaaaatgca aaatatcttt tggatcgctg tttgtcaata
     7501 cgacatccgc agaatcgatt gcgatatctg tccctgcacc gattgccatc ccgattgaag
     7561 cacgggctaa acttggcgca tcattgatcc catcacccac catgattact tttttccctt
     7621 ggtcaaggta tttctggaca acagcttctt tatcatctgg taataaaccg ccataatatt
     7681 cttcgatccc taaataatct gctactgctt gggcagcgtt tttattgtca ccagtaagca
     7741 tgacaggagt gatttttctt tctttcaatg ttcggataaa ttcttttgct tctgttttta
     7801 ctacgtcacc taaagcaacg attgcagcta gatgattttc taaaatcagg aagctgatgg
     7861 tatttccttg ttcttggtaa ggtttcaata atgcttgttc tacgtttaat ccgagacgct
     7921 caacttcttt ttcattaact attttcactt gttgtttttt gactgttgcc tctagcccga
     7981 ctcctgaaag attttttaga ttttcagctt gataaggctt gatttcatgt tctttcaaat
     8041 aattcataat gccaacagcc aatggatgat tggcattcgc ttccaaggct cctagatatt
     8101 gaaggatttc atttttattg aacgcatcat caagtacttc gacacccgtt acagtaaatt
     8161 ttccttctgt tagtgttcct gttttgtcca gcatgatcac atctagatca ttggcttgct
     8221 ccagtgcatt gcggtttttc aaaagtaatc cgtttttagc tgctattgat gtggaacgag
     8281 caacaactaa tgggatagct aaacctagag catgaggaca ggcgatgata aagactgtta
     8341 ccatacgttc taatgcatta ggaagatctg tcaagaacaa ccaggcgatg aatgccagga
     8401 tacccacaat taatgcgaca taaaacagcc attttgccac tttgtcagat aatgactcta
     8461 gcttggattt ttctccttgt gcttttcgca ccatttccat aaccttcgct agataaccat
     8521 tttcaccagt tccagttact tcgatttcga tcgttccatc cccattgata gaaccgccaa
     8581 tcactttgtc attggcttgt tttttgattc ctcttgattc gccagtcaca gctgattcgt
     8641 caacaattgt ttcacctttc aagattacac catctgttgg cattttatca ccagaccgga
     8701 cgatcaaacg atcaccttcg tttacttctt tcaatgaaac tgtctcttct tttccatctt
     8761 tcgtcaagcg tttcactgat tctggcagta attctgctag tttttgtaaa gcattgctgg
     8821 cattcgacac agcattcatt tcaatccagt gacctagtag cataataacg atcaaagtag
     8881 ccaattccca gaagaaatcc atgacgtgtg tatgtggatt gagcagattt gcgataaacg
     8941 aatagacact ataaatatat gcaactgtaa tccccatcgc gatcagtgtc atcatagcag
     9001 gacttttttg tttcaattcc atcttagctc cacttaaaaa tggctgaccg ccatagataa
     9061 atagtatagt tgctaagatc aatacgagcc aatcagaacc aggaaacgtt acttgaaatg
     9121 gaaattccat tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg ataagcgata
     9181 gccaaaattt ttgtttgaaa ttccccatgt gcatggaatg atccatgccg ccgtgttcgt
     9241 gatccatgtg catcgaatgg tccatttgtt tgctgtttgc tatatctcca tggttatgtc
     9301 ccatgtgcat ggaatgatcc atctgactat ggtcatgttt catctccatc tgtttttcat
     9361 gagattctgt actttctttg tttactactt cttttttatg ctcatgtttt ttctccatat
     9421 cgttcccatt catttttact ccatcccttc tcttactttt tcatgtgtcg atttaagcac
     9481 agggagttca acaatacact gacggaacta aacgccatcg ctcctcctgc aatgattgga
     9541 ttcaaaaagc ctagtgcggc aaaaggaata ccaatcgtat tatagacaaa agcccagaaa
     9601 agattttgtt tgatttttct caatgtcaac ctagacaact cgattgtttg tgcaatggat
     9661 gccaaactgc tattcatcag cgtcacatcg gctgtttcca tcgcaatgtc cgttccactt
     9721 cccattgcca taccgacatc agctaatgct aaggccggtg catcattgat tccatcgcct
     9781 gccataccga cttttttgcc atctttctga agtttttcta cgtaggctgc tttatcctct
     9841 ggaagtactt cagcaaagat atgctttgga tcaataccaa cttgtttacc aatcgtttcg
     9901 gctgccagtt tgttgtctcc tgtcagcata aaaacatcta atcctttatt ctggagctgc
     9961 ttgattgctt gtttgacttc tagtttgatt tgatcagcca cggcaatcaa cccgatcact
    10021 tgcttttcat cagctaaaaa catgactgtt tttccctgct gttctaattc taacgcgtgt
    10081 tcttggtatt catcaaatga taggttcaat tcaattaatc gtttacgtgt acccgcaaaa
    10141 tagcgtactc cgttaatcgt accgctgatt ccagcacctg ggtgggctgt aaaatcaatc
    10201 atatcgtatg ttgctgcttc ttgcagtttt ccgtattcca cgattgcttt acctagtgga
    10261 tgttcagaag cttgttctaa agtataaaaa atagatagga cttctttaga accgactaga
    10321 tctgtaactt tgggctttcc ttctgtaatc gttccagttt tatccaaaac aatgctgtct
    10381 aagtgagcag ctgcttctaa tgcttctccg cctttaatta aaattccatt tcgtgcacca
    10441 acacccgttc ctaccataat agctgttgga gtcgctaaac ctaatgcaca tggacacgca
    10501 atgactaata cagagacact atggatcaat gcgagttgcc aatcaccagt gatcaaacct
    10561 gtagctatga gcgtgataaa cgcgatgact aatacgatcg gaacaaaaat ccctgagatc
    10621 cgatctgcga ttttttggat tggggctttg ctcccttgag catcttccac catttgaatg
    10681 atttgcgcta atactgtctg cttgccgact tgtgtaactt ccatgtgaag tagaccattc
    10741 gtattgattg tcccgccaaa gagcgtgtca tccggattct tttctacagg aagattttca
    10801 ccagtaagca tactttcatc tatcgcactg cttcctgaaa taattcgacc atctgctgga
    10861 atctgctcac caggacggat gactagttga tcaccaacga ctacttcttc aatcgctaac
    10921 gtttcttctt tcccgtttct tatcacttgg gcggtttttg tttgaagaga catcaattgt
    10981 ttgattgcat tacctgtttt cgtttttgct gtatgttcca aatatttgcc gagcaaaatc
    11041 agcgtgatga tcatactgct gctttcaaaa taaagatctt gggggtgtcc acggaaaaat
    11101 ccgttgtaaa tgctcaatgc aaaggcagcc gatgttccaa ttgctaccaa tacatccata
    11161 tttggtgctt tcgtcttgat tgcatggtac gcacctttat aaaaacgagc accgacataa
    11221 aattggacag gagcagacag cactaactgg acgattggaa aatgaaaaaa gtgaacaatc
    11281 gccgcatggc ttcctaacat catagcaatc attgacaaca tcaacggcaa agtcaaaacc
    11341 gtactcaata taagatcgaa tagcattttt ttcaaatacg cttgcttttc ttctgcaatt
    11401 ttttgtttgt gggcttcatc gtacaaaatt gcaccatagc cgatgttttc tacgctttgg
    11461 attaattttt cagtggtcgt cccttcatat ttgacacttg ctttttcagt ggccaagttg
    11521 actgtggcat tgaccacacc aggttgtccg ttcagctctt tttcgatcct agcagaacaa
    11581 tttgcacaag tcatacctgt aatgacgaac gtatccattt tggtttctgc tgttgccatc
    11641 aaatcacctc tgctttataa cccaatccat tgattgcttg acaaatcttt tcggattgaa
    11701 cttttgtttc atcaaacttg accgtaccgt ttgctttttt caaatttact ttcacttttt
    11761 gcacgccgtc aagtgcgctt actgtttctt ctactcttgc tgcacaatga ttgcagctca
    11821 taccttcgat tgaaaattgt tgtttcattt tttcttcctc ctattgatgt tgtttacatt
    11881 cacattgacc tgggatacag ttacattcga tcgtatctac tggttgcttt tgttccagtt
    11941 gtttcaaaat ctcattgata tcttcttgag tcaaagtggc ttcttctact agatccgcaa
    12001 tcgtctctcc aatccttttt gcacagatat gggaaaaaag attttccgtc gcactgcgaa
    12061 ctgtttccat ctccgagaca gctggataat aaatgaattt ttttccttct tgttctgtcc
    12121 agatcacttc tttttttacc aaacgaccca gcaaagtttt gatcgttgct ggtttccaat
    12181 ccatcgaatc agctaagatc tgcgtgattt cttgggctgt cgtctgtcca agtgtccaaa
    12241 taacacgcat gatctcccac tctgaatcac tgatctttac tggcgttttg actttttcca
    12301 tttatttcac acctttcgtt tacaaatgta atcattatct tatgaataca gattacactt
    12361 gtaaacaaaa actgtcaatt atttttgctc tctattttca atttagttaa aaattcttgt
    12421 ttctctcgtc ttgttttaag gatgatcagt ttatttcttg cattagctac ttggatcagc
    12481 tgctgtattt tcggttcatt gttttcttca aaatgccaga taaaagataa aaattctaag
    12541 tattccctgt ttagtttctc cgaaaatgat tcaggcatat ctggccgaga ttttttgttt
    12601 atctttcttt ttagagaacg ccaaactact cggtagatgg cgagataacg cggcacttta
    12661 agccaaatga tcttatctgc ttctttaatc ctttcctcta atgtagaagt ataatttcct
    12721 tcaacgatcc atgacggatt agataccata aacaattgct gttcttgcaa aaaccacttt
    12781 tttgcctgaa tactgtagtc tgttgtatgc cataaaaggt ctagcggcag tagcggaaag
    12841 tcggtgattt ctttgatctt ccttgcgagg gtcgattttc ccgacccact ttgtccaatc
    12901 acgatgatct tcattcttct ccctcacttc tctttggaat ttggttcgat cataccactt
    12961 ataacgccgc aaaaaaacaa gggactgtga caaaagtcgt gtcaaagtcc cttgtt
//
LOCUS       DBNICI010000073        12946 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.073, whole genome shotgun sequence.
ACCESSION   DBNICI010000073 DBNICI010000000
VERSION     DBNICI010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.073"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(77..149)
                     /locus_tag="AC1ZCC_001672"
     tRNA            complement(77..149)
                     /locus_tag="AC1ZCC_001672"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="AC1ZCC_001673"
     CDS             530..1099
                     /locus_tag="AC1ZCC_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HIQ1629922.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="AC1ZCC_001674"
     CDS             1086..1946
                     /locus_tag="AC1ZCC_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIQ1629923.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /gene="yycF"
                     /locus_tag="AC1ZCC_001675"
     CDS             2136..2840
                     /gene="yycF"
                     /locus_tag="AC1ZCC_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HIQ1629924.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="AC1ZCC_001676"
                     /gene_synonym="yycG"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="AC1ZCC_001676"
                     /gene_synonym="yycG"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HIQ1629925.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="AC1ZCC_001677"
     CDS             4677..5993
                     /locus_tag="AC1ZCC_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YycH family regulatory protein"
                     /protein_id="HIQ1629926.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="AC1ZCC_001678"
     CDS             5994..6863
                     /locus_tag="AC1ZCC_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HIQ1629927.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="AC1ZCC_001679"
     CDS             6918..7727
                     /locus_tag="AC1ZCC_001679"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIQ1629928.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="AC1ZCC_001680"
     CDS             complement(7830..8459)
                     /locus_tag="AC1ZCC_001680"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIQ1629929.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="AC1ZCC_001681"
     CDS             8629..9921
                     /locus_tag="AC1ZCC_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIQ1629930.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="AC1ZCC_001682"
     CDS             complement(9997..10659)
                     /locus_tag="AC1ZCC_001682"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIQ1629931.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /gene="groES"
                     /locus_tag="AC1ZCC_001683"
     CDS             10884..11168
                     /gene="groES"
                     /locus_tag="AC1ZCC_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIQ1629932.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="AC1ZCC_001684"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="AC1ZCC_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIQ1629933.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4315 a         2238 c         2802 g         3591 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacg
//
LOCUS       DBNICI010000074        12794 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.074, whole genome shotgun sequence.
ACCESSION   DBNICI010000074 DBNICI010000000
VERSION     DBNICI010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.074"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            185..619
                     /locus_tag="AC1ZCC_001685"
     CDS             185..619
                     /locus_tag="AC1ZCC_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIQ1629934.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            720..1811
                     /locus_tag="AC1ZCC_001686"
     CDS             720..1811
                     /locus_tag="AC1ZCC_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HIQ1629935.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            1841..2518
                     /locus_tag="AC1ZCC_001687"
     CDS             1841..2518
                     /locus_tag="AC1ZCC_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HIQ1629936.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            2850..4145
                     /locus_tag="AC1ZCC_001688"
     CDS             2850..4145
                     /locus_tag="AC1ZCC_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIQ1629937.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            4135..5595
                     /locus_tag="AC1ZCC_001689"
     CDS             4135..5595
                     /locus_tag="AC1ZCC_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1629938.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            5731..7227
                     /gene="glpK"
                     /locus_tag="AC1ZCC_001690"
     CDS             5731..7227
                     /gene="glpK"
                     /locus_tag="AC1ZCC_001690"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HIQ1629939.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            7241..9064
                     /gene="glpO"
                     /locus_tag="AC1ZCC_001691"
     CDS             7241..9064
                     /gene="glpO"
                     /locus_tag="AC1ZCC_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HIQ1629940.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            9065..9781
                     /locus_tag="AC1ZCC_001692"
     CDS             9065..9781
                     /locus_tag="AC1ZCC_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HIQ1629941.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            10087..11562
                     /locus_tag="AC1ZCC_001693"
     CDS             10087..11562
                     /locus_tag="AC1ZCC_001693"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0045910 - negative regulation of DNA
                     recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HIQ1629942.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGRNRRKPSFVDCS"
     gene            11564..12016
                     /locus_tag="AC1ZCC_001694"
     CDS             11564..12016
                     /locus_tag="AC1ZCC_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIQ1629943.1"
                     /translation="MDMSMKLIHQAERYLAEKAYRTQKKEFLPKTAVTNRKENKKERQ
                     LFAKGDRIFVNEYQKEALVYEDIGEDTIDVYLDKKIIHVPRQRVRLVRSAEDLYPTGY
                     DLDSLFIDYKTRKRQRDLERGSKKAHKVLVKEMRKRQEERRVNDENSK"
     gene            12142..12696
                     /locus_tag="AC1ZCC_001695"
     CDS             12142..12696
                     /locus_tag="AC1ZCC_001695"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIQ1629944.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
BASE COUNT         4288 a         2125 c         2901 g         3480 t
ORIGIN      
        1 cgactttttc acaacctctg attcacggtg ttccagaagg agttttgtcg aaaacaagcc
       61 gaaattcttt gaagatggct acttcttttt ttcaaatcta gacagaggag gcggacatta
      121 ttccgtgttt tttgacaaaa aataaaaatc atactatgat atgtacgcca atggagggga
      181 tgaaatggaa aagccaattg aaaaagcgtt agatgaatgt cttttcttta gcgtgaaaaa
      241 gctagatcgc atgttgaata aattagctga tgaagcgttt agaaggacag gtctggcacc
      301 aacttatggt tttattttat tgatattaaa agaaaaagat ggaataccac aaaaagatat
      361 tgcacaaatg ctctattccg ccccatcaac gattgctcgt tttgttgaaa aattagaata
      421 taaaggatac gtcaaaacag ttagcgaagg tcgcttgtca ttagtttatc ttacagaaca
      481 aggacgccaa tttgctaagg aagtcgatgg tagctgggaa gaacttcatc agtcatacaa
      541 agcagttttg ggaaatgaag caagtaaaga attagcccag acaatgaatg atgcaacaga
      601 taagttacaa aaaaaataaa attttttaag caaattagtt gcatgtacaa ctaatcgagt
      661 ggtttaatga tagatgattc tcgataggtt aatcagagcg aattatggga ggaatgacaa
      721 tgtcacaatt tgatcaatca attactttac cttcaggagt tcaattgaag aaccgattga
      781 tgctgtcgcc tatgacgaca caactcagct ttttcaatgg agtgatcacc gaagatgaga
      841 tcacttatta taaacaacgt tctaaaggtc ttggtgctgt tattactggt gctgcaaatg
      901 tccaagatat cggaaaaggc tggccaggtg aactcagcat cgctcatgat gaaatgattc
      961 ctcgattaag tgagctagcg aaagcaatcc aaggagaagg cgcaaaagcg attattcaga
     1021 ttttccacgg tggacgaatg acaagtcgag caactttatc tggagaacag cctgtatctg
     1081 caagtgcagt tgcagcaaaa cgtcctgatg cggaaacacc acgagcaatg agtgaagaag
     1141 aaatcgttga aacaatccaa gcatttggca aagccacacg tagagcgatt gaatcaggat
     1201 ttgatggaat cgagttgcat ggagcaaata cgtatttgat ccaacaattt ttctctccgc
     1261 attcgaatag acgagaggat caatggggcg gtagcttgga aaatcgttat cgttttattg
     1321 aagaattatt aacaacggtt ttctcagcgg ttgaacaata tgctaaaaaa ccattcatcg
     1381 tagggtatcg tttctcacca gaagaatatg aaacaccagg tatccgtttt gaagacacga
     1441 tctggcttct tgaacgatta cgtgagtcaa aactagatta tctgcatgtt tcattaaata
     1501 tgtatgatcg aatagctcgt tctgataaat acaacgataa atcgattctt gaagtggtcc
     1561 ataacacgat tcaaggaaag atccctttag ttggtgtcgg tgggatccgc aatcgcgaag
     1621 atgtagagca ggtattgaaa tatgcagaac ttgcagcaat tggacagcaa atgatcgtcg
     1681 atcctgactg ggcagagaaa ttattggaaa accgtgagga tgaatttgta acgaaacctt
     1741 ttgaagaagc atataaagag ctttatttgc caagcccatt atataatttc ttgaaagcac
     1801 gttatcaata acgcaatttt tatagaaaga ggatgggaaa atgaaagtat tgatcgtatt
     1861 aacaaatgta gaaaagtacg aaaagatcga gcgtcctaca ggtttgtggt taagtgaact
     1921 gactcatttt tatgatgtat tagtaaaaaa tgggatcgat gcagattttg tcagtcctaa
     1981 aggcggatat gtacctttag aaccgcaaag tgttttgaat atggatgaaa tcgattgggc
     2041 ctattatgaa gatgaagatt tcagaaatcg ggccttggga aataccttac gtcctgatga
     2101 tgtggatgca aatgattatg cagcggttta ttatgctggt ggccacggaa caatgtggga
     2161 ttttccagaa gcaatagatc ttgggaaagt cgcttcacaa atctatcgaa atgaagggct
     2221 ggtctcagct gtttgtcacg gtgttgtagg attgttgcca gttacagatg agtctggaaa
     2281 atctatttta gctggacgta cagtgactgg gttcagtaat gaagaagagg aagcaaatgg
     2341 tacaacgaat gaagtaccat tcttagcaga agatgctttg aaagaaaaag gagcaaacta
     2401 tcagtctgga gcagcttttt cagaagtggt tcaaattgat gggcggcttc ttacagggca
     2461 aaatcctcaa tctgctcatc aattaggcga agcagttgtt gaagcattaa agaattaaaa
     2521 ataaatgatg tgtctattct aactattaca ttacacacgc taaaactcgt cttttcgttc
     2581 ttttctgttt cctatttgga cagaggtacg taaaaggcga gtttttttct tttataagag
     2641 tacatttttt gaaacatctc ttcagaaatg atactcttca attaggtcca ctagtacatt
     2701 attgagaagc agacctagaa cacggtatga tggagagggt tgattctgct gcatcgctat
     2761 gtatcataag gttgaaagcg agttatgaat actgtgtttt ctgattgaga agttagcagt
     2821 agaacacctt ataagaaaga ggaatgcaga tgaacattgt aattgtcggc gggtcgtttg
     2881 cagggattca tgcggctatt tatcttcgcc aattgatgcc aacaagtgaa atatatttga
     2941 ttgaaaagca atcgaagtta ggatggattc ccagcgggtt caatttgata ttaaaaggaa
     3001 aggttaccag tgaaaaacaa ctttcttgga tcacgaaaga agaactgact gaccgctatc
     3061 gaatccatgt ttacacagaa aagacagtaa taggattgaa agaaaaaaaa gtcgttttag
     3121 caggaggaaa aaaattggat tttgatcgtt tgattctagc gactggttca aatcaaaagt
     3181 ttcgaaatat ttctgcagaa tcaacgctta ttcacccagt taaaaagata caaaacgtaa
     3241 cggctcttca gcaaaagatc atgaatgcaa agaaaattgc aattattggt gcaggacaag
     3301 caggaatcga aatagctgaa gggttggcat caagaaaaaa acaaatccat ctttacgaaa
     3361 gccgaaagtc aattcttttt cgttatctag atcctgaaat gacagaaccg ttagtaaagg
     3421 aaatgaaaaa tcaagggctt tcactttttt tagaagaaca agttgtgtct ttgaccgaaa
     3481 atgaaaccgc tattgtagag acggaaaaaa gaaaggagaa ctatgatctt gttttgctgg
     3541 ctaatcattc tcgtcccgac aatcagatgt gggaagaaca attaacattg aatgatgatg
     3601 gaacgatctg ggtagatgac tacttgcaaa cgtctcaaaa agacgtttat gcgattggcg
     3661 atgcgatcca agtaactttt cgtccaacaa atgaaaaaat gtatgtttct ctagtaaata
     3721 acgctgtccg tacagcacga aatgtcagca agacaattag tggctcacag acaaaggatc
     3781 agggaaccta tcgtccagtc ggcaatcagt ggtttggcta tttcttagga agcgtaggtt
     3841 tgacagaaga agagagcatt ttttatcccc aaaaaattga aacaggatac gtagactaca
     3901 agcttacagc aataaatcaa agcaaagtta aactcaaatt tctatttgat tctgaaaaaa
     3961 agattcttgg agcacagctc caaagcaaaa aagagatgtt ccaccttctt gaccaactga
     4021 caattgcagt agaagaagga tggacgtttg aacagttaga agaacatgag ctatttttcc
     4081 agccagaata ccgtgtgcca gatatggtgt ggtcaaaggt ggcgaatact gcctatgaag
     4141 attgaagcat tattagaaag aaaagagcag ttacagatcc tgattttgcg aaaccttgtt
     4201 ctgcaagggg gaacagcctc tatcaatggt ttgagagagc atgtccaact gtcaaaagcc
     4261 tcgtttgatc aatatttgga agatattgaa ttgattggcc gaatgatgga aaaaaaagta
     4321 gaagtacagc gaaatgagta tcaggcgctg cttgtgttgg acgaagatgt aagtttggaa
     4381 aaaattctat tgtttttatt gcaggaaagc ttgaagttta agatgctcgt ctatttatta
     4441 gaacatcagc aagtatccat cgttcgatta gcgacagcgt ttaatatcag tgagtcttct
     4501 gtttttcgaa agatcaaaga attgaaccag ctcttagagg aatttggatt acaaatcaaa
     4561 aacggtcaat tatacggaga agagctacag atacgatatt tttattatga actattccaa
     4621 tatatcccgg aagatcagcg accgcttttt ttgcaaaata caccagaaaa aagaccgttc
     4681 attctaggat tggaccgtgt attagaaaca acgtttacgg catcagcaga agcacagatt
     4741 gcttgctggc tcgggattac gaaaaaaaga ctattgaatg aaaaaagtac gtacgcaact
     4801 ttgaaagaaa agaaattact ctatcagtcg gaccggcttt atcaagcaat cgatccaatc
     4861 atcgtgatgc atcttagtcg aacagcagca gaactgaatg tatacgaaac aatgatgttt
     4921 tacagctttt ttgtttcttt ttcgatagta gacgaagaaa tattttatca gtatgatttg
     4981 acacggagca aaaagttacc aaccgctgtt ttagatactt acattcggga aacgatgtta
     5041 tggcattatc gtcctcgtcg tttgaaaatc aaagaagaaa aggcagtagg ctaccaaatt
     5101 tcccagatca acaatgaatg gtttttcttt gttggaaaga tcgaagtcta tgagcgtgat
     5161 cggcttttgg aacagcagca gaaaatgctc gggcattcct taacacagtt actggcgaaa
     5221 ttacaggaaa cagcggtgca gcaattgccg aggaaaagag ctgaagatag tgagcttagt
     5281 tacctgatga tccagtatgc caatgttctt ttgatgatcg atttttatat tgcaaaacca
     5341 gtagtgattg gaatcgattt agaaagtctg ccgatttatc gtattgcttt tcaacaatat
     5401 ctgatcagag aactgagggg aatcggcggt attgagattg gaagttatga agaaggtaaa
     5461 gaatatgatt tggtgatcac attttgccag agaaacaaaa aacagtgtga atattatctg
     5521 tcagaatttg cgtcacctta cgacattatt cgattgaaaa gaagaataga gacgctgaaa
     5581 aaagaaaaga attgattaaa aaatagcttc ataataaaaa actgtcaaaa aaagaaaaaa
     5641 agaataaaat tttgacagtt tcttttttta aaacagtagt atgataagag tagttaaaga
     5701 aagcgtttgc atataatgga gggaatcaca atgacagaat caacgtatat catggcgatt
     5761 gaccaaggta caactagctc acgagcaatt atctttgaca agaaaggaag acacatcggc
     5821 agttctcaaa aagagttcac gcaatatttc ccgcaagaaa gttgggtgga acatgatgca
     5881 aatgaaatct ggaattctgt acagtcagtt attgccggag cgttcatcga atcagggatc
     5941 aaaccaaatc agattgctgg gattggcata acgaaccagc gtgaaacaac agtaatatgg
     6001 gaaaaagata caggacgtcc aatctatcat gcagttgttt ggcaatccag acagtcagct
     6061 ggaattgcag ataacttaaa aaatgaaggc tatcaagaat ttttccacga aaaaacaggg
     6121 ttagtgatcg atgcctattt ctcagcaacg aagatacgct ggattcttga tcatgtagaa
     6181 ggcgcgcaag aacgagctga aaaaggcgaa ttgatgtttg gaacgatcga ttcttggtta
     6241 gtatggaaac tgaccgatgg acaagcacat gttactgatt actcgaatgc cagtcggacg
     6301 atgttgttca atatccatca gctagattgg gatcaggaga ttttagattt actaaatatt
     6361 cctcgagtga tgttaccaaa agtcacttct aactcagaag tttatggtta tacgcaaggt
     6421 tatcattttt atggaagcga agcaccaatt tcagggatgg caggagacca gcaagccgca
     6481 ttattcggac aaatggcatt cgaaccaggc atggtcaaga atacttatgg tacaggttca
     6541 tttattgtca tgaataccgg tgaaaaaccg caattatcca aaaataattt attgacgaca
     6601 attggttacg gcataaatgg aaaggtttac tatgctttag aaggaagtat ttttgtggct
     6661 ggttcttcga ttcaatggct gagagatgga cttcaaatgt tgcaaaaagc tagcgattca
     6721 gaagacgcag cgaaacgctc tacaagcgaa gatgaggttt atgttgttcc tgcattcgtt
     6781 gggctaggtg ctccttattg ggatcaggca gcacgagggg ctatgtttgg cttgacacgt
     6841 ggtacgacaa aagaagatat cattaaagct actttacagt cgattgctta tcaagtgaga
     6901 gatatcatcg atacgatgca agatgacact ggaatcaaga ttcctgtatt gaaagtagat
     6961 ggcggtgcag caaacaatga atatctgatg caattccaaa cagatatctt gaatgtaccg
     7021 atccaacgag cagaaaactt ggaaacaacg gcgctagggg cagcattttt agcaggatta
     7081 gctgtcggtt attggaaaga tacagacgag atccgcgaat tttatgaagc tggcaagcta
     7141 tttgaagttc aaatggagga agagcgccga gaaaaacttt acaacggttg gaaaaaagca
     7201 gtaaaagcta cccaagcatt tgaataagga ggaaatccat atgttttcaa acaagacaag
     7261 acaagatagc attcaaaaaa tgcagcaaga agaattggat ctgttgatca tcggtggcgg
     7321 aatcactggt gccggtgtgg cagtccagtc agcagcatca ggaatcaaaa caggattgat
     7381 cgaaatgcag gattttgcag aagggacttc ctctcgctcg accaaacttg ttcatggcgg
     7441 tattcgttat ctgaagacat ttgatgtgga agtagtagct gacacagttg gtgaacgtgc
     7501 agtcgtacaa agtattgccc cacacatccc aaaaccagat ccaatgcttt tgccaatcta
     7561 tgagggcgaa ggagcaacaa ccttcaatat gttctcggtc aaagtagcaa tggaccttta
     7621 tgacaaactg gcaaatgtga caggaaccaa atatgagaat tatacgctga caccagaaga
     7681 agtattggaa agagaaccgt ttttgaaaaa agaagggcta aaaggcgcgg gtgtctatct
     7741 agatttccgc aacaatgatg cccgtttagt gattgataat atcaaaaagg ctgccgaaga
     7801 tggggcttat ctagtaagta aaatgaaagc ggttggcttt ttatatgatg gcgatcaaat
     7861 cgttggcgtc aaagctcgtg acctgctgac agatgaagtg atcgaaatca aagcaaaatt
     7921 agtgatcaat acgagtggtc cttgggtaga taaagtacga aacttgaatt ttacgcgtcc
     7981 agtctctcct aaaatgcgtc caactaaagg gatccattta gttgtagatg cgaaaaaact
     8041 gcctgtaccg caacccacat acttcgatac aggaaagcaa gatgggcgga tggtttttgc
     8101 tattccaaga gaaaacaaga cttactttgg tacgacagat acggattacc aaggagattt
     8161 tacggatccg aaagtcacac aagaagatgt agattatcta ttggatgtga tcaatcatcg
     8221 ctatccagaa gcaaatatca cattgaatga tatcgaagca agctgggcag gacttcgtcc
     8281 actgttgatt ggtaattctg gttctgatta caatggtgga gataatggat cgatttcaga
     8341 caagagcttc aataaagttg tcgatacagt aagtgaatat aaggaaaaca aagtttctcg
     8401 tgctgaagta gaagacgtgt tgaaccattt ggaaaacagc cgtgatgaaa aagcaccttc
     8461 tacgatttcc agaggtagtt ctttagaaag agaaccagat ggtttgctga ctttatctgg
     8521 tgggaaaatc accgattacc gtaagatggc agaaggagct ttgcgattga tacgccaatt
     8581 attaaaagaa gaatacggaa tagagacgaa agaaatcgac tctaaaaaat atcagatttc
     8641 aggtggaaac ttcgatccaa ctaaattaga agaaacagtg acagaattag caaaagaagg
     8701 agtagcagcc ggtttagagg aagaagatgc tacttatatc gctgattttt acgggactaa
     8761 tgcccgacgt atctttgaat tagcaaaaga aatggcacct tatcctggtt tgagtctcgc
     8821 tgagtcagct cggttacgtt atggtttaga agaagaaatg gttttggccc caggcgatta
     8881 tctcattcgt cgtacaaatc atctgttgtt tgaacgagat cagcttgatg agatcaagca
     8941 acctgtgatc gatgcaattg ctggatattt tgaatggaca gaagaggaga aggcgcaaca
     9001 gactaaacgt ttagaagcat tgattgcaga atcagatctg cgggaactaa agggggagaa
     9061 ataaatgaac agtgatatga ctcaaatcat gggggagttt attgggacac tgatcctagt
     9121 tttgcttgga gatggtgtgt gtgcggctgt gaatttaaac aaaagtaaag cacaggcttc
     9181 tggctggatt gtcatcgcat ttggttgggg actcgctgta acaatggctg tctatatttc
     9241 tggattcatg ggcccagctc atttgaatcc tgcagtctct ctagctatgg cgatgacagg
     9301 ggcaattagt tggaatcttg tcgttccttt catcattgcg caagttttag gagcttttgc
     9361 aggggccatt cttgtttggc tttcttattt gccacattgg aatgcaacga aagatgaaag
     9421 cgcgatttta ggaacgtttg caacaggtcc tgctatacgg aattatccag caaatgtgat
     9481 cacagaatta attggtacat ttgtattagt tcttgggttg ctagcttttg gacaaaacga
     9541 atttgcacct ggaacgaacg tattcgctgt aggtggattg atcttagcca tcggtctttc
     9601 attaggaggg ccaacaggat atgcaatcaa ccctgctcgg gactttggtc ctcgattagc
     9661 acatgcggta ttgccaatag ctaataaagg aacttcagat tgggcatatt catgggtacc
     9721 aatcgcaggt ccaatgatcg gagcaattat cgcggtaggt gtctattcat taatggtgta
     9781 aatctataaa acatactaaa gtttgacaac caaaatgtgt cttgctatag ttaagtggaa
     9841 aataaataag acctagaaga atggggtatg ttttgtgagt gtagctggta tctaatcagt
     9901 aagtcgaata gccaataaac aaagtttatt tggtgtgctt atgaaacggt acctgtcagg
     9961 aaaacaatat ctttgtattc taataaggat aggacatgac ttttttaagc tgtgttctat
    10021 ccttttattt atctctaggc ccttatttat tttgggacag gtatctcttc aagaaaagga
    10081 gagaatatgg acaacgaaat gattagtcaa ttacaatttg atcggatcaa aaaggaaatc
    10141 caagcaagag caattggaaa ctatagcaaa aaacgaatca gcgatatgac ggtctcgaca
    10201 aacctacaaa ctgtcttgga tcgtcaagaa gaaacaagag aagcacgctt gatcttagaa
    10261 agcagtcagc atgttccatt tatggggttg ccgaggatcg atgcactgac agaacaagtc
    10321 aaaaaagggt tgattctgca gccaaccgat ttgatcgaat atgcggattt tcttagaagc
    10381 agtcggatga tcacaaagtt ttttgataag aatcaatacc agacaccctt gttgtttgct
    10441 tatagcaaac atcttcctga tttgataaat gtagaagagt tgattgacca gaaaataaaa
    10501 aataataaag tcagtgacga cgcgtcacgt aatttgcgta aagtacgcaa acaattacaa
    10561 ctaatcgaaa aagaaatcca gtcaaaatta ctgaaattct tgcgtcatcc gaaaaacaaa
    10621 gaaatgatcc aagaagcaat gattgttcaa aaaggcgaat actatacgat tcctatcaaa
    10681 gccagctata aaaataaaat agatggaacg attatcgatg aatcaaataa aagaacgacg
    10741 gtttttattg aaccaactgt gatttctaaa ttgaatgaac actatcagtt gttaaaagct
    10801 gaagaaatca gtgaagaata tcaagtcctg gcggcattga ctggtgctat cgcagaaaac
    10861 gaagaagtga tcgatttatt gatcgaaacg atcacggtgt tagatatcat ctttgctcgg
    10921 gcaaaattta gccgagaaat caatggaagc acacctagaa tcaataaatc ggaacatatc
    10981 attatcaagc aaggcagaca tccattttta cctgagcatg cggtaccatt ggatgttgaa
    11041 atcggtaaag actatcgagg cttgattatc accggagcaa atgccggagg caaaacagtc
    11101 gtgttgaaga cagtcggttt attgactttg atggcgatgt tcggtatgca agttccggca
    11161 aaagaaggga cagagcttgc tgtctttgat gagattttcg tcgatgtcgg tgaccaccaa
    11221 aatttagaaa atgcgttgag tacattttct ggtcatatgc aaaacatcgc tgctattttg
    11281 aaaaagataa aaaggaatac gttggtactt ttagatgaga ttggcagtgg aacagagcca
    11341 aatgaagggg ctgcactagc gatcgcaatc atggaatcta tgtatgagca aggggcattg
    11401 atcattgcca cgacccatta tggagaaatc aagaaattcg ccggagatca tgaagatttt
    11461 gtgccggctg ctatggcttt cgatcgggaa gcacttcgtc ctaaatatca gttgcgggtt
    11521 ggaagaaaca ggagaaagcc aagctttgtg gattgctcat aaaatggata tgtcgatgaa
    11581 attgatccat caagcagagc gttacttggc ggaaaaagcc tatcggacac agaaaaaaga
    11641 atttcttcct aagacagcag ttacaaatag gaaggagaat aaaaaagaac gtcagctctt
    11701 tgcaaaaggt gatcggatat ttgtcaatga ataccaaaaa gaagcgttag tttatgaaga
    11761 tataggtgaa gatacgattg atgtatattt agacaaaaaa ataatccacg tgcctcgtca
    11821 gcgtgttcgt ctggtgcgtt cagcagaaga tctgtacccg acaggttacg atttagacag
    11881 tctgtttatc gattataaaa cacgtaaacg gcagcgtgac ctagagcgag gatcaaaaaa
    11941 agctcataaa gtactagtaa aagaaatgcg caaacgacag gaagaaagaa gagtaaacga
    12001 tgaaaacagc aagtgattat ctcaaattag ctcataatat cagtgtctgt aaagaaaaat
    12061 aaaaacagtg aaatcattgg ttttattccc cgtgtaagct aaaataaaaa gaaaagagtg
    12121 ctgaggaggt agggcgataa aatgaaatta agcacagact ggcgtaaaga aatacaaacg
    12181 attccgaatc ttttatcgat attccgtatt tttttgctgc cgatttatct ttattttgtg
    12241 ctgagacagt cattttatat agcaggagcg gtcattgttg tctcaggttt gtctgattat
    12301 ctagacgggg tcattgctcg gagatataat caggtaacag acttagggaa ggtgcttgat
    12361 ccgtttgcag ataaattgac acaacttttt ctgattttgt ctatggcatg gtatcgaccg
    12421 tggctttggc tgttgtttgg tctattcttg ataaaagaag gctttatgtt tgtagctgga
    12481 ttaatcgggc tatctaaaaa tatcaaatta agtggagcga agtggtatgg caaagtagcg
    12541 actgctgtca tctatgttgg catgatcctt ttgctgcttt tcccagaatt accaacgctg
    12601 tgggtgagag tcatttttgc cgtcatcact tacggtcttt tacaatcttt tgtcttgtat
    12661 gctgtcgagt accgaaagat gtttcaaaga aaataaaaaa ggggtgtgac ctatgtcgca
    12721 ttcctttttt tgataaatag actcccagta gctacttctc gataataagc caaccgaacc
    12781 aaaaacagga gaag
//
LOCUS       DBNICI010000075        12784 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.075, whole genome shotgun sequence.
ACCESSION   DBNICI010000075 DBNICI010000000
VERSION     DBNICI010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.075"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="AC1ZCC_001696"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="AC1ZCC_001696"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIQ1629945.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="AC1ZCC_001697"
     CDS             complement(2261..2512)
                     /locus_tag="AC1ZCC_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HIQ1629946.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="AC1ZCC_001698"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="AC1ZCC_001698"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HIQ1629947.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="AC1ZCC_001699"
     CDS             3462..3680
                     /locus_tag="AC1ZCC_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629948.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3969..4481)
                     /locus_tag="AC1ZCC_001700"
     CDS             complement(3969..4481)
                     /locus_tag="AC1ZCC_001700"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HIQ1629949.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="AC1ZCC_001701"
     CDS             complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="AC1ZCC_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIQ1629950.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6934..7377)
                     /locus_tag="AC1ZCC_001702"
     CDS             complement(6934..7377)
                     /locus_tag="AC1ZCC_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HIQ1629951.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7378..8166)
                     /locus_tag="AC1ZCC_001703"
     CDS             complement(7378..8166)
                     /locus_tag="AC1ZCC_001703"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIQ1629952.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8163..9104)
                     /gene="rnz"
                     /locus_tag="AC1ZCC_001704"
     CDS             complement(8163..9104)
                     /gene="rnz"
                     /locus_tag="AC1ZCC_001704"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HIQ1629953.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9281..9868)
                     /locus_tag="AC1ZCC_001705"
     CDS             complement(9281..9868)
                     /locus_tag="AC1ZCC_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HIQ1629954.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10089..11402)
                     /gene="obgE"
                     /locus_tag="AC1ZCC_001706"
     CDS             complement(10089..11402)
                     /gene="obgE"
                     /locus_tag="AC1ZCC_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIQ1629955.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11690..12700
                     /gene="gap"
                     /locus_tag="AC1ZCC_001707"
     CDS             11690..12700
                     /gene="gap"
                     /locus_tag="AC1ZCC_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIQ1629956.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
BASE COUNT         3529 a         2866 c         2272 g         4117 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aagaagttaa
     3721 acttttacgg gggtgtgaca aaaatcggtc acatcctttt tttcgaataa acggtgttct
     3781 aaagccagtt tttcggtatt aagtcaaatt gcggaaaaag tgatttttta aacttctact
     3841 ggtctttgct tgtttccatt tataaaaaaa tagcgaccct caaaagtcag attctctatc
     3901 tgacttttga gggtcgcttt aaaatcgctt ctttaagaat aacatcaaga atagcttctt
     3961 ttttctgctt aatattccat taatgtaatg atgtcatatc ctttgatttt gtcgcgtcca
     4021 tggagatcca tcaattcgat caaaaatgcg cagcctacga ctacaccgcc taattgttcg
     4081 atcagttcga tcgtcgcttt gattgttcct ccagtagcta gcaaatcgtc acaaatcagc
     4141 acacgttgac ctggtttaat ggcatcctta tgcaatgtca atgtatctgt tccatattct
     4201 aatccataag tcacttcgat ggtttctcga ggtaacttcc cttttttgcg gactggggca
     4261 aaaccgactc ctaactcata agcaacagga catcctacga tgaatccacg ggcttctggc
     4321 ccaactacca tatcaattcg tttttctttt gcataattta cgatttgttt agtagcttcc
     4381 cgataagcgt caccatctgc catcagtggg gaaatatctc gaaacacaat tccttttgac
     4441 ggatagtcgg gaatactggc aatataatct tttaaatcca ctctttgttt cctcctaggt
     4501 tgttaaccat ttctttatgg taggaatatc gctcaaaagc aaaaattcct caactttgat
     4561 tttattgagc cgtcgctggt ataatctgct ctcagtcaaa ggatgacttt ctgggctagc
     4621 gactttttgt aaaacaccgt tctctattgt aacaaatttc aattcaaaaa acacctgtat
     4681 cataaaaatt aataattttt cagggatacg caaatattga gctattgctt ttattttatg
     4741 acgtatatca attcgttctt gagaatggat caatttaaac aattttgcgt actgttctct
     4801 tgtgcctacc ccattcaaat aggcctcatc aggtgaaatc cccatcagat aaacacgatt
     4861 gaatgaataa gaccgaaaaa cttctttcag atccgctaaa tcatctggac aatccataac
     4921 aactagagag tttggttttc ttttttcaat tattgccttt acagaatcta gtccttcgaa
     4981 aacaatcgca gcttcctgga taatctcagg cacgaatttc aacgtcgaag aatcaaatgc
     5041 taaataaagg ctaccttccg acgttcggct ctgttcacgg aaacgttttg cccgccaatc
     5101 aaatacttgc agaccaccca cagaaaaatc agataccatc agctgcggtt tcttacgccc
     5161 gttccattca ttgatcgaaa gctttcctac cacatctacc tgattattca gcagttcttc
     5221 ttcttgagta ccaaaaccaa aagcgaccgc atcaagctga ctcgatgcat cggaaaggac
     5281 aaatttcaga tgctgttggt ttgctccgat tcttttgata ttttctgctt ggacttgtcg
     5341 aaataaaaac tgaggcaaag gattatccgt accaaatggc gccaatagtt tcagctcatc
     5401 aattcgctct actgttactt catttggcag caatacttca tcaattctca actcaggccc
     5461 tctcatcaag tcaatttgat gatcgactat atattggttc atcttctcct gtagtatagt
     5521 gacattttca cttggcatcg ttaaaccgac tgctgcatga tgtcctccga agtaagtaaa
     5581 taaatctcgc atctggtcaa gcatttcaaa aagattcaag gcttcaatac tacgtccaga
     5641 tcctttagct gatccatctt cttttaaagt aaggacaagc gttggtttac ccgtttcatt
     5701 cattattttc ccagcaacaa tgcccaaaac gccttcatgc catcctcggt ttaccaatag
     5761 atggatatga ttctccttat tgaccatcgc taatgcttct tgtgttatct gctctacgat
     5821 tgcttttctt tcttcattga tctcattcaa tttttttgct tgaacggatg cttcctcttc
     5881 atcaaacgtc gcaagtaacg agacggcagg acgtggatcg cccatccgac cgattgcatt
     5941 tagtctggga gcaatcgaaa aaccgatcgt tgtttcgtct gcgtccctca tttttacgcc
     6001 gctttcttca aatagcgcct gaagtcctat ccgttctgaa tgatgaatgg catcgattcc
     6061 taatgaaaca aggattcgat tctcatcagt caatgaaact aaatcggcga tcgttcctat
     6121 cgctactaaa tccaaaaatt ctgcaggcgg ttcttccaat aatgcagaag ctactttgaa
     6181 tgctactccg actcctgcca aatctggaaa tggatagtgc cccgctggat gacgagggtg
     6241 aacaatcgca taagcgtctg gtaaattctc aggtagttcg tgatgatctg taatgatcac
     6301 gtctacacca gcttgctgag cgtatgctac cgcatcattt cctgctaccc cgttatcaac
     6361 agtgatgatc aactggattc cttcttcgat cttttcttgg tataccgatt ggtttggtcc
     6421 atacccatgc tcaaatcgat ttggcaggaa tacttctact tctgcaccaa gtaattccag
     6481 tgtttctttc attaccgttg cactggtgat cccatctgcg tcataatcac cgtaaacgag
     6541 gattttttct ccactgatta cagcttcttg gattcggctg attgctttat ccatatcgtg
     6601 tatcagatac ggatcatgta attcttgttc ctttggatgt aaaaatcggt tgatatcctt
     6661 ttcctcatgg taacctcttt gccacagtaa cttaccgaca aatggagata atttcagttc
     6721 acggatcatt tggagaaagg attcagaagg ctctgtctcg cttaacttcc aatcaaaatt
     6781 tgcttgtctc actttctcac tcctaactta gttattatag caaaggaaat cagcaaaaaa
     6841 aagcagaaag aaagaataaa aacagatcag atccgctaga cagatgttca gcaggacctg
     6901 atcttttgta cgaccattta tgaaatatcc ctattagtca aaataattga agctttgttt
     6961 gggcgattga gttttttgtt cttccggcgc tttcaattga ctgatttgtg cggttttcgc
     7021 ttgtaactcc gcctgataag cagcttccaa ctccgcacgt tcgtttctaa actcgcgttg
     7081 ctgttcttct tttgcttctt caattttctt ttgaagttct gtttgatatt ctgacatttc
     7141 ctgagtcagc tgtttgattt gctttttttg ctgccatagt gttgccgaag cagtcaaaaa
     7201 tacaatcagt gcaccaatca gcgcagaacc gatgatcact aagatcaatg gcgcagaaaa
     7261 ggaagtgaag ccaaaactta caggaacatt tttattgttc aatacagcaa aaagaacaat
     7321 aatcaaaact aaaacaaatc caagaatcac attgctttgc tttttcaatt ccttcaccta
     7381 ctttttgtta aaaattccgc tggctaaata atcaccaatc gttggaaaca gcgtataaaa
     7441 ttttgatgca acttctaaaa taaacggttg attgatttcc cgttttggat gtctcatatt
     7501 gcggacgatc atttttgcca acttggcagg ttctaaaacg atttgtccga ctttttccaa
     7561 atagcttccg cttggatcag ccttatcgaa gaaatttgtc tcgattggac ctggattcac
     7621 tgtcgtaaca gctacgccaa gtggtttcaa ttctaatcgc aaagcgttgg agaagccaag
     7681 gactgcaaat ttcgtggcag aataaaccgt tgattttgca gttgccattt ttccagccat
     7741 agaggcgata ttaatgatat gaccttgtct tctttctgcc atatcgatcg caaatttttg
     7801 tgtcaaaacc atcattccta agatgttgac ttcaaacata tcgtatgctt ttccaaggtc
     7861 gaatgtaagg aagtcttcga aaatcccaaa acccgcattg tttaccagaa tatcgacgcg
     7921 cccgacttct tccatcacct tttcatataa ttcgtccacg ctttcaggat tgctgacgtc
     7981 taccttgaaa gcataagcct ctttgccgct caagcgtctg cactcgtctc tgacaccttc
     8041 tacaaatgcc attcttcgtg cgcaacttat aacgattgct ccttgctttg ctgcttcata
     8101 acagatttgt gcgcccagac cgcctgaact acctgttact aaaacaactt tatcttctaa
     8161 attcatgcct cttcctcctc gcgaaatgga atctcaatgc tatcaaaatc tttcataatc
     8221 gtcgtgtttt caaacacttc ttgtgcttct ttttcaagct ctaaagctgc ttttcctaaa
     8281 tatctcgcac tgatatgagt aagtagcagc cgttttgcct gcgcttcttt tgcaacttca
     8341 gcagcttgat gagtcgtgga atggaaatag ttgtgcgcca ttcttgcttc atctttatta
     8401 aatgtgcttt catgtaccag aatatcgcta ttttccgcaa gcacaacgga attatgtgtt
     8461 tttctcgtat cgcctaaaat agtcacgatt cgtccttttt tatctggacc tacgaaatct
     8521 tttccattga tcgtccgccc atcttctagt tggatcgttt caccttgctt cagtttgccg
     8581 tacagtggtc cagctggaat acctaattct tttagacgat ccacctgcaa ttctccttta
     8641 tgatcatgtt caaccacacg ataaccaaaa ctgtcgatcc catgatttag tttttttgta
     8701 taaacagaaa attgctgatc agtaaaaatc ggggctgttt catttagctc aatgaatttc
     8761 agcggataac ttaaacgcgt ttgcgaaata cctaaagaga cttttatgta ttcctctatc
     8821 ccttttggcc cgtaaatctc taatggcgta tccccacctt gaaatgaccg gctgctgatg
     8881 agcccgggaa gtccaaaaat atgatcgccg tgtaaatgtg taatgaaaat tttaccgatt
     8941 tttctcggac gaatagtcgt tcgcaaaatc tgcatttgcg tgccttcgcc acagtcgaac
     9001 aaccaaactt cattgcgctc gtctaaaagt ttcaatgcta tacttgtcac atttctatgt
     9061 tttgcaggaa cacctgcacc tgttcctaaa aattgtaatt ccataatcta cctgactttc
     9121 tttaagttcc tctttaattt tagggtaaaa agaagggata atcaaccgta agtcgagaat
     9181 gggttcaaat taaaaataac gttctaaagc gatagcgttt tctttgaaaa ttactataaa
     9241 aacagcagcg aacccgtttg agatcctctg ctgtttttta ttaatttgaa gatccagaac
     9301 tttcattgtc gttggaagtt tgcggatgtt tgtccttcca agtcttttgt gcagcttctt
     9361 tcgcatgatt ttcagcttct ttcgcatcat gcacatcttt ttcgacatct tcataatcca
     9421 agcgagtaat ttctccgcct aattccatca ttaccaattg attcagtggg cgattgtcct
     9481 cttcatcagc aatcaagatc agtcctgtag ctccttcacc gattttattt cctacaaagt
     9541 cgaacacggt ctgggcatct tttatttctt tggcatcttg tgtcgcaccg atcattcctc
     9601 cggcaaacca tcctaataaa atccctagcg gaccacctaa tacaccaacc aacattccga
     9661 ttaatccacc cgtagaagat ttgttattac cggtaaagtc gataaaatct tctattttga
     9721 attgatgtgt cccattagga tcatggctga caactgccat ctgttctcct ttaatcgaac
     9781 gaacagcatg taactttttg atttctgaaa atgcctgata tgcctggctt tctacttgaa
     9841 aatttaaaat gattactcgt ttgctcatta aaaacgcccc tttcatacct tcttgttatt
     9901 tattttacct ttaattgaaa aataagaaaa gaaaagcact ctttttttcc aataaaatag
     9961 ggctgtgaca aaagtcgtat cacagctcct ttttccgaat aaacggcgtt cttgaagctg
    10021 atcacttctg tcacaacctc ccttaaatct gaatctatat tttttggtat ttgtttttat
    10081 ttgcttgcct attcgacaaa ttcaaattca aactcaccga tacgtacaag gtcgccatct
    10141 ttcgcaccgc gtgcccgaag agcttcatcc acacccatac cacgtaattg acgagcaaaa
    10201 cgcatcactg tttcatcatg atcgaaattc gtcatttgga ataatttttc gatcttctca
    10261 ccagataaga tccacgttgc atctgaatca cgatcgattt ggaactcagg accttctgga
    10321 ttgaatccat agtgtacagt ttcttcttcc agttcttctt cgtacaatgg gaattctggc
    10381 gttacttcta acaaatctgc tgtagcattc aataacgcgt ccaatccttg tcgagtcact
    10441 ccagaaattg gaaatactgg gatgtcatca gcaaattcat cttctttttc cttgttcagc
    10501 tgttctttga atttcactag attttccggt gcatccggca tatccatttt gtttgccacg
    10561 atgatttgcg gacgctctag caagcgtaag ttataagtag ataattcttt gttgatggca
    10621 agatagtctt cataaggatc tcgtccttcc atcccactca tatcgatcac atgtaaaatt
    10681 actcgtgttc tttcgatatg acgaaggaat tgtgtaccca gcccaacacc ttgcgaagct
    10741 ccttcaatca agcctggcaa gtctgctgcg gcaaaacttc tgccatcact tgtagtaacc
    10801 attcctagat taggtacgag cgtcgtgaaa tgataagcgc cgatttttgg acgagcagat
    10861 gaaataacgg atagtaaagt cgatttcccg acagatggaa aacctacaag tccaacgtct
    10921 gccaatactt tcaattctag ttcgattttt cgctcttgtc ctggttcacc gttttcagca
    10981 atttccggtg caggattccg aggagaggca aaacggatat tccctcgtcc accgcgtcca
    11041 ccttttgcca caactagtgt ttgaccattt tcaattaaat ctcctaaaag agcaccagtc
    11101 tccgcatctc ggacagtggt tccttgtggt actttaacat aggtatcttc tgaaccacga
    11161 ccgtgcatcc ctttactcat cccattttca cctggctgcg ctttgaaatg gcgattgaac
    11221 cgaaaatcca tcaatgtacg taacccttca tcgacgatca agatgacatc gccgccacga
    11281 ccaccgtctc cgccagccgg tccgccgtca ggaacgtatt tttcgcgacg aaatgcaacc
    11341 attccgtctc cgcccttgcc agctttcaca tcgatcgtta cttgatctaa aaacatggac
    11401 ataattaagt cctcctatta ttcatttatt tatttgactg cctgaatttc cacaataaac
    11461 aaaagtctta cttattttat cggtgaaatc tgattttgtc tatcatttca gcaaaaagat
    11521 tgtttacctt cttctttttt tatcatttgg taaggtttcc aacctttttt ctgctaccaa
    11581 gtataatttt ttgacaaaat attctttgcg tagcagaata acagtgtatt aattgtatta
    11641 attgaggatg ccgtttcaca gcacccctaa taaaggagga acttttgata tgacagtaaa
    11701 agtaggtatt aacggatttg ggcgtattgg ccgtctggct ttccgccgca tcaaagaagt
    11761 ctcagatgat attgaagtag tagcaatcaa cgacttaaca agtccaacta tgttagcaca
    11821 attattgcaa tttgactcta ctcatggtac ttatcctggc acagttactg caacagaaga
    11881 ctcaatcgta gtggatggcg aagcaactcg tgtttatgca gaacccgacg caagcaaaat
    11941 cccttgggca aaagaaaacg gtgtagatat cgtactagaa tgtactggtt tttatacatc
    12001 tgaagaaaaa gcaaaagcgc atttagatgc tggtgtcaaa cgagtagtta tttctgcccc
    12061 agctggtgct atgaaaacca tcgtctataa cgtcaatgat gatacattag atgaaaacga
    12121 caaaatcatc tcagcaggtt catgtacaac aaactgttta gcacctatgg cttatttctt
    12181 aaataatgaa tttgggatcg aagttggtac aatgacaaca gttcatgcct acacttccac
    12241 acaaatgctc ttagatggac ctgtaaaagg cggtaatttg cgtgctgctc gttcagctgc
    12301 tgacaatact atcccacatt ctactggtgc tgcgaaagca atcggcttag ttatccctga
    12361 gctacaagga aaattacaag gacacgcaca acgtgttcca gtcgttgatg gttcattaac
    12421 tgaattggtt tcagtattga aaacaaaagt aactgctgat caagtaaacg aagcaatcaa
    12481 aaaacacaca gttgacaatc cttcattcgg gtatgatgac cgtcaaatcg tttctagcga
    12541 tgtcattgga acaactcaag gttcgatctt tgacccaacg caaacagaag taacaactgc
    12601 tggtgatttc caacttgtta aaactgttgc atggtatgac aatgagtatg gatttacttg
    12661 ccaaatgatt cgcttactag aaaaattcgc taacttgtaa tttgatagat caatcgataa
    12721 aagaagcctt tgactggatc cagtcaaagg cttctttctt ttcaggctct tcgtcaaatc
    12781 gtgt
//
LOCUS       DBNICI010000076        12777 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.076, whole genome shotgun sequence.
ACCESSION   DBNICI010000076 DBNICI010000000
VERSION     DBNICI010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.076"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(83..802)
                     /locus_tag="AC1ZCC_001708"
     CDS             complement(83..802)
                     /locus_tag="AC1ZCC_001708"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIQ1629957.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(851..2497)
                     /locus_tag="AC1ZCC_001709"
     CDS             complement(851..2497)
                     /locus_tag="AC1ZCC_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative polysaccharide biosynthesis protein"
                     /protein_id="HIQ1629958.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            2676..3266
                     /locus_tag="AC1ZCC_001710"
     CDS             2676..3266
                     /locus_tag="AC1ZCC_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIQ1629959.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3358..4863
                     /locus_tag="AC1ZCC_001711"
     CDS             3358..4863
                     /locus_tag="AC1ZCC_001711"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HIQ1629960.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(4947..6299)
                     /gene="celB"
                     /locus_tag="AC1ZCC_001712"
     CDS             complement(4947..6299)
                     /gene="celB"
                     /locus_tag="AC1ZCC_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HIQ1629961.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6299..6817)
                     /locus_tag="AC1ZCC_001713"
     CDS             complement(6299..6817)
                     /locus_tag="AC1ZCC_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629962.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(6833..7159)
                     /locus_tag="AC1ZCC_001714"
     CDS             complement(6833..7159)
                     /locus_tag="AC1ZCC_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HIQ1629963.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7172..9151)
                     /locus_tag="AC1ZCC_001715"
     CDS             complement(7172..9151)
                     /locus_tag="AC1ZCC_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HIQ1629964.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9172..9486)
                     /locus_tag="AC1ZCC_001716"
     CDS             complement(9172..9486)
                     /locus_tag="AC1ZCC_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HIQ1629965.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            9654..9947
                     /locus_tag="AC1ZCC_001717"
     CDS             9654..9947
                     /locus_tag="AC1ZCC_001717"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HIQ1629966.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10025..11137)
                     /locus_tag="AC1ZCC_001718"
     CDS             complement(10025..11137)
                     /locus_tag="AC1ZCC_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HIQ1629967.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11290..12138
                     /locus_tag="AC1ZCC_001719"
                     /pseudo
     CDS             11290..12138
                     /locus_tag="AC1ZCC_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12491..>12777)
                     /locus_tag="AC1ZCC_001720"
     CDS             complement(12491..>12777)
                     /locus_tag="AC1ZCC_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HIQ1629968.1"
                     /translation="GAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVAD
                     SSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4029 a         2570 c         2162 g         4016 t
ORIGIN      
        1 gccatccgaa agtcagacgg acaagtctgc ttttcagatg gcgctacaac tactctgctt
       61 attcttttag ctatccattc aattattttt ctctcacttg ttctagctct tctttggtca
      121 tgggtcgata ttgcccttta ttcaaatctt taggcagcca aaattttccc atacggattc
      181 tttttaaata taagacttct tttcctactg ctttgaccat tcgcttaact tggtgaaact
      241 ttccttcatg caaaatcaga cggataattg aagtttctgt ctcttgatct acttgatcaa
      301 tttggagttt tgccggcaat gtctgctcat cgccatcaat aatcagtcct tctgcaaata
      361 aacggatatc ttcttctgtc attataccgc taacctcagc tagatattct ttctccacat
      421 gttttttagg tgatagtaac tgatgggcaa acaccccgtc attcgttaaa atcaagaggc
      481 cctctgtgtc tttatctaat cggccgactg gaaaaagatc ttcccgataa tcctggtctt
      541 ccaataaatc gattacggtt tgatcatgat tatcctgagt agcagaaatg actcctgcag
      601 gtttatgcaa aatgtagtaa aaatcttttt gatacgtaat gatttctcct aaataagtaa
      661 ccgcatcttc gaattcattt acttgcgctt tatcgctttt caccacttgt ccattgactt
      721 cgatcgttcc ttttttgatt aatcctttga cttctttgcg gctgccaagt cccatatctg
      781 ctaaaaactt atctaaacgc atgcgtttcc cccatttcaa aagagcatca atctagctct
      841 tctgattcac ttattttatt tttaatttat gtcgtaaacg cgctattcct ggtcctagta
      901 gtctttctgc caagcggatt ttcaaagcta aataaccata cactaaagcc cctatacctg
      961 ctactacaag aatcaacaat agagactgga atttgctatc ttcgtttaaa aaagcaccaa
     1021 aaatcatctt tgtgatccac gaagccaaaa gcataatcaa ggtaagaata aagatcagca
     1081 atgtcctgcg ccagacaaaa cttggtttaa agcgggctac ttcatggatt ctctttaaga
     1141 tcaaggtaca agacacagca aatccgatca ttgtcgccaa taaagggccg tatacttcaa
     1201 ataaacgaat ggctgggtat tgaagaagga gtttcactag aaacccaata accaaataat
     1261 taattgccgc cttgttttca aacattcctt gcagcatgtt ggaaactagc atatatagtc
     1321 ctaggaagag tcctacaaaa cttgcttgga tcagtacatt acttcctaga gaatccggtg
     1381 tataaaataa tgtatttagc ggataggcaa gtaacataac accaaatgtc gccggaaaca
     1441 taaaaaatga aaacaactgt aaatttccgc ttgtcaattt tgccagacct actcgatctt
     1501 tcaacgtaac agcttctgta atcaacggta acccagtaga cgcgatcgat gttgccaatg
     1561 caataactac catcgtcaac ttatctggat tggcactaaa aatcgaaaaa agatccagta
     1621 actgtgcatt ggaatactcg gtggatgcag acatgatctt cataaaagtg aactgatcga
     1681 ctaatttgaa gatcgttacc cctgaaccca caataataaa cggaatcgct tctttaacgg
     1741 tatctaacaa caattccttc gttgcaatat ttattttgtt ttcgctgtag tgaataaacg
     1801 ccgatgtata cgcttgatgt ttcttcaaaa aatacaaaag gacaagaata cttgctagca
     1861 taccgataaa tgccgcgaaa gttgattgcg tgaccgccgt cacatagtca ccatccatga
     1921 ctttcatgat gatgaatgta gccaatagca tataaaaaat acgtgccacc tgttctacaa
     1981 tctgcgacag cgcataaggc atcatttctt gattgccttg caaataccct cgtatcacac
     2041 tcatacaagg aaaaaccaat acagcgatac tcaaagaacg catcgttgga atcagttctt
     2101 ctccgccacc agaagcatga gctagccaag gagaggcgat gtacatgaac aaagcaaaca
     2161 acaggcctaa cccgcccatc atctgtaatg cacggataaa taatcgtcgt gaagtgccat
     2221 attcattcaa tgaattatag cgtgcagttt gttttgcaat cgcagcaggt attcctgctg
     2281 tagagatcag tagaaacaat gcatatatat tgtatcccat attgaataat ccattggcag
     2341 ccttagcatt ttcgcccatc caagcatacc agggaatgat atagacagcg cctagcaatc
     2401 ttgaaatgat attactggcg gtcatccaag cagagcctcg tgccattttt tcttgcggtg
     2461 ttaattgatc taatgctggt tttctttgat tatccatcgg tcagattcca actttctaca
     2521 aaacattact atcattttaa ttggtaaaac agaaactggc aatcgatttt atataacttt
     2581 tataagaaca attgtttttt tgaattcggt ttcgtttttt tggtaaagta aagaaatata
     2641 cagcctttta tggctaacat ttataggaga tatcaatgac acaagaaata aaaaaactac
     2701 ccgttatatt tttaggtctt ttctttatca cagttggatt gaattggttc ttgctccctc
     2761 acgatatagc ttccgccgga gtgggcgcca tcggacattt agttgaaacg gccttttcta
     2821 tccaacgctc agttactgtt tggagcatca atcttatgat gctgcttcta gccgctgtcc
     2881 tattaggaaa atcagtattt tttaagactg tagcgggaag tctgcttttt cctgttgttt
     2941 tagagattgt tccaaaagtg gaaatgatct cctcacattt cttttcactg atttcaggaa
     3001 gtcttttgtt cagtcttggg gtttacacat tgtacacagt cggggcctca aatggcggta
     3061 taacgatacc gccgattatt ttccacaaat tttttcggct tccaatgccg caaggacttt
     3121 tacttaccaa tagtcttatc gtctttttga attattatgt ttttggattg ttggagacac
     3181 tattcgttct cctatctatt acagtcagtt cattgtttat gaaaatactc atccgcttgt
     3241 cccctgtgtc aagaaaattt gaataaagct ggattttgtc atagcggaaa caaacgatat
     3301 attaaaaaga gtgtgctata ataaaccaag aaattgaata aagcgaggga tcaatcaatg
     3361 agcttctctt tagaagacat ccgtcattta ctattaaaag aaaatctatt aaaagaattt
     3421 gtttcaacaa gaggctggca tcttgatgta ccagaaaaag ctgaatttct tcagttaagt
     3481 tatgattcaa gaaaagctga tgcttctact ttgtttttct gtaaaggaat gaatttcaaa
     3541 gaagaatatt tggattcagc aatcgaacag ggtattcaat attatgtatc agaacaacca
     3601 tacgaaaatt cagcagtagg aatcatcgta acggatatcc gtaaagcaat ggcgcttctt
     3661 gctatggctt tttacgacta tccgcaaaat aaattgaaag tgatcggatt cactggaaca
     3721 aaaggtaaaa caactgctgc gtactttaca aaagcaatct tggatcacac aacaaataaa
     3781 aaaacagcat tgctttctac tatgaataca acgttagatg gcaaaacata tttcaaatcc
     3841 catttgacaa cgcctgagtc attagattta tatcgtatga tgtcagaagc agttgaaaat
     3901 ggtatgactc atctagtgat ggaagtttct tcacaagctt acaaaacaca acgcgtatac
     3961 ggcttgactt tagatgtcgg aatcttcttg aatatctcgc ctgaccatat cagtccgatc
     4021 gaacatccga catttgatga ctatttctat tgcaagcgac aattgatctt gaattcaaaa
     4081 acagttgttt taaatcatga aagtgactat tttgatttac ttagggaaac agccgatcta
     4141 ttccagattc caacgatcac ttatggtcgt tcggaagaaa gcaattatca agtgatccgt
     4201 tcagataaag gtactcacgg ttttacttta tcttctcatg aagatcgctt agctatctgc
     4261 gatactgctt atgatatttt gctggctgga ggattcaacc aagaaaatgc agccagtgcg
     4321 atcattgccg cagcactaac aggtgcctcc agtcaagatg cacaagacgg attaaaagaa
     4381 gctcgtgtac caggccgaat ggatcaattg atccaatcca atggagcgca tgtctatgtg
     4441 gactatgcac ataattatct aagcttgaaa acattgctga aatttgccaa aaatgaacat
     4501 ccagatggcc gtgtaattgt tgtattaggc agcccaggaa acaaagcaat ctcccgtcga
     4561 catgattttg gaaaagtgct atcagaaaca gctgacatcg cctttttgac ggcagatgac
     4621 ccagcatttg aagatccgaa aaaaatcgcg gaagaaatca atgaagcaat tactaatcca
     4681 gatttgatcg ttcattatga aatggatcgt cctgaagcga ttcgacgcgc tttagcagaa
     4741 agcgctccgc aagattcagt cgttattgcg ggaaaaggcg tggatcccta tcaaaaaatc
     4801 aatggtgtag atgaacctta tgaaggggat tatgcaatcg tgaagcggtt gattgaagaa
     4861 taatggtatt tatatactag tggtttccca tttctaagaa aaaaagggac tgtgattttt
     4921 tgtcacagtt ccttttttct tagaaatcat tttaactttt cactagctaa ttctgctgcc
     4981 ttttctcttt cttcatttaa taatttatta tcatagaatt tgataaatgg gaaccagata
     5041 atgaatgcga tgattgcaca aatgactgct aacacagcag ctttccaatc accaccacta
     5101 ccaacaaatg ctccgatccc gactggtgaa ggccacggca tctgtgcaag catcggtttg
     5161 acgatttcca gccgaatggc aaaataagcg agtgtagcag aagccatcgg agctaagaaa
     5221 aacggaaccg ctaaataggg attgtagacg attggcatcc cgaaaatgat tggttcattg
     5281 atattgaata atcctggtac aagtgaggct tttcctaaca ctttcagctg atcagatttc
     5341 gcgaggaatg caataaagat gatcaatcct aaagtcgcac ctgatccacc aacggttaca
     5401 taagaattgt tgaattctcc tgccaacgga taatttgcgc cttgtgcgtt agctgccata
     5461 ttagctaata cgatcggggt caagaaagaa gtgatgatat tcgctccatg gatccctacg
     5521 atccacaatg catggatcaa gaaataaatc accatgacac caagccatgt attcgtcaac
     5581 tgcgtaacaa acccaaatgg gatagcaatc attttgaaga tatccgttcc tagtgcaact
     5641 aataatccat tgatgatcat caccacaaac gcaatgacaa atgttgggat cagagcagtg
     5701 aaagaacgag aaactcctgg cggaacaaca tctggcattt ttatgaccca atttctcttg
     5761 acacacatac aatacaattc gaccgcaata atcgacataa taatagctgt aaagattcct
     5821 gaagttccta aacgttcgac aaaagagccc atcctaatac catttactac agtttctgtc
     5881 tcattgatag aagcaatcaa gctgatttta ccatcagtca tgatcaattc agggatacac
     5941 ataaagaatg caaaaacaga tagtaatgcc cccgtgagcg gattaacatt taatttttgt
     6001 tcatctcgtt caattgacgt taattcataa gcaaacacaa tggcaaagta aatagataaa
     6061 atccccatcg tcattgtatt gacgatcatg tacaagtcac taactttgaa aaaagttgca
     6121 ttaaaaaatc cttctaaaaa tgggaacggt aatggcaaaa tatttaaaac caaaaacatc
     6181 gagcctacaa tcgtgaaagg aatagaagcc atccccgctg ccatgacagc ccgaacgatt
     6241 ttaagttgcg caattttccc cataggtccc atcaggtatt tctgcaaaaa atcaaacatt
     6301 agttattctc ctttcctaaa tataacgctt tttcatattg tttaatacgg atatactgtt
     6361 ggtcttggtt gagtgaaatt ttctttaaac gatactgtat aaatgcactg gcaaaaatac
     6421 cgcttacaac aagaatgaaa acaaataact gtcctccatt gttatttgcc ataaaaggat
     6481 acaagaatgt aaatcctgag gtaaacaaca ctggaatcac taataaatta aagacactct
     6541 ggaacaaaaa ataccacttt gtccatggaa cgttcgtttg gtgttttcga taaagcttta
     6601 cttgttctcc tactgcaggc aaaatagcta aaatcagaat catcggtaaa ctacttccta
     6661 aacttttagc tgatagaagc agaatcatcc attgcatgtt catgaaaaag aaaaaggcag
     6721 tcaggtaacg tacgattaga taacgattga aatacatgtt tttcaaacta agttgctttt
     6781 tttctaattc gattttcctt tttactgtat cctgcacaat gatccttcct ttctatttat
     6841 ttttttgata caaataattc atttcctgtg caacttccaa taatgtcatc gtcgtcatca
     6901 gatgatcttg tgcatggacc atgataattt ccatttctat ttttgtcccg ctggcatatt
     6961 ctttcagaag ttgagtttgt gactgatgcg catgaagaat ctcttcattt gcttgtcgca
     7021 acttctcttc tacttcttca aacatctctt tacgcatcga gcgaaatgct tcatgtatca
     7081 gtgtccgtgc atttccgcta tggaaaatga tatcaaaaga agtcacttgt agtgtttcac
     7141 tgtttatttt ttcagccatc tgttttcctc cttattcgat cagtttgata aatttactgc
     7201 tgaaattctc aaaaataggt tcagctaaga tttcctgctg tacttttagc tggtcaacta
     7261 acctaacgat tgctttagtc acgaccgtaa ttccttcatt ttcgacataa gaaggcgaaa
     7321 ctaaaaaaac aaaatgaatt tcttgatatt gatcccactg catcccagaa ggaatcaatg
     7381 caacggctac ctttgtttcg attccaactg gaacagcggg atgtgggact gccactgtat
     7441 cactgaatat gatttgtccc atattttcac gttgctggat ctgctttcgc atttctctta
     7501 tatatccttc ttcttcattt atgctcagct tttgcagcaa atcatgaaga atttcttcct
     7561 ttttcggttc gtacgtataa gtcttgaaaa atttttcaga caactgttcg gtgtaaatct
     7621 gctttttttg ctgtagagaa aatgctggta tcggtctatc tgaggaatga tagggtatag
     7681 attctccaac tacctttcgt attttacgaa catcatcttc attcaaaaaa atactgacat
     7741 gtaaaactgg aattttgaaa aacattgtcg aaagatcaat agaagaaata atcaggtcga
     7801 ttccttttaa agttgtttca ttgatctcat aatagccttt tacacttacc actgtaatat
     7861 ttttaccgaa ttcactgact acgcgattct ttaatagttg ggcactgccg taacccgttg
     7921 cacagataat caatgcatgt agtttatgcg cagctctttc tttttccaat gccgccatga
     7981 gatgcaaagc caaataggcc cattcatctt cattgatggt atagtttttc aaagccggca
     8041 tgttgctgaa atgccttttt actgcgtcaa agactttggg attttctttc tttatctcct
     8101 cagtcaatgg gttctcaatg cgaatacctc ggctcaatcg aatcatcatc ggtgacaaat
     8161 gatcgagtag tccattgaaa agctggagat cctctactaa tgaaaaatga aattcctgag
     8221 acaatctttt cagaactttc gctaattcct ttgatagttc ctgatcattt tgattttctt
     8281 tgtgattcga cttagccatg agatgtaatg tcaaataagc aatttcttct tcgggaaacg
     8341 atacacctgc gattacctca atacgttgaa cgatccgctg cgccacttga tactctactc
     8401 gcttagaaac ttcttgttcg atgcctaatt ccttgatttc aaacccttct cggagccgtt
     8461 tgatccccaa agccaagtgt aaagtcagat tctgtatgat aaaatccgaa acttttaact
     8521 tagcttctct tgtttcatcg agaatgataa tgactagctc ttcaaaactg atgtctggga
     8581 aaaaacggct ttgccctaaa tattctttca atgaatttcc ataatggttt ccaaaaaaag
     8641 tatccatgat aaaatgccgt cgatttcttt cttccccttc gatccatgtc ccttgaccat
     8701 gcttagaaac gatttttagt gaatatggaa tcaatcgctc cttgatttct tgcatgtcct
     8761 ttgtcaaact ggaacgactg atataaagct cttctgccaa atcatccata aaaagaatgg
     8821 catcttccaa aagcaattgg ttcaatatat acttttgtcg atcttcggac tcataaaatc
     8881 cttgcggttc tctagcatat ccaggtacta tttcttgccg actcaaaaac aagtcaaatg
     8941 tcagtctttg taaaatatgc agttggtagc cataacctgg tttagcaatg atcttacctc
     9001 cgtttttttc tactaactct ttgatccgct gcaagtaatt acggattgtc cgatcagata
     9061 ataacaattc agaagctaaa cgttgactgg tgacatactc cttcttgtga tcaatcaaat
     9121 atctcaacag caatttttcc ttctgtttca ttatcatcca ctcctattcc atcatatgtt
     9181 ctcttcgatc acctctgcta acttctggat gcctgtaggg attgggacat acgcttggaa
     9241 agggatacta acaacaggct ttccaacaga ttctcctaat tttttgaatt tatccaaaaa
     9301 catcgtcgtt tgcgggctga tcagaaataa atcaaaatca ctattcttaa tcatattatc
     9361 gccttctgta gctgaaactg catctacgag tacctcttct cttttttgct taaagaaatc
     9421 cgttgtcttt gaggccatca tcgaggatga cattcctgcc gcacaaataa tcaatgcttt
     9481 tttcataaat caatcactcc ttttattttt ataaaccaag catatcttgt aagcgttttc
     9541 tattcaaaat atatatttcc gtagcattgc ggaaaaaatg tgtatcatat cttgaataat
     9601 aatttctggc accgtgatat actagtgata gaattttttc aggaggggat ttagtgagta
     9661 tcacagtgaa tattttgtat acaagtacaa atggacaagc cagaaaattt gcagaagaaa
     9721 tggtcgaaag aaacattgtc aaagcgattc gtgcagaaga aggaaacgag aaatatgact
     9781 attactttcc actagaagat ccagaatctc ttttactgat cgaccgttgg aaagatgaag
     9841 aagcaatcga aaagcatcac aaatccgaaa tgatggctca aataaacgaa ctccgtaaaa
     9901 agtataaatt aaaaatgaaa gtcgaacgtt tcactgattt accgtaaaaa gatacacatg
     9961 gaatgatagc aatttatcat ccatgtgtat cttcactttt tccttatttt ttattcgatc
    10021 tcatctaatg attcaaaccg tgtttttctc ttatttttcg gataagtatt tcagtcaccc
    10081 caatatacat gattcctaaa cataccccaa ggatattctc aaaaatgcgg atcgttactg
    10141 ctcctggaac cccgaataaa ctggcagtga ttgccaaagc accaaaagaa ttaaaaactg
    10201 ttttatatct ataggtagaa caaacaccta aagctagacc acctaatata ccgacccagt
    10261 taaaaggaat aaattggctg ataccaataa ataaaacaca tccaatcaat gagccaatga
    10321 ttcggtctac tgctcgttct ttgaaaccaa tcgacatcaa tccatagcta gatacaatag
    10381 aagaaaatgc aaaccctgcc cacatcaatc gctgaaaagg gacatactct cctgcaacga
    10441 tcaaaagact aatgccaaat gcatagatga gcatccaaat tttttcctgc gaatacatgc
    10501 ctttcaaaaa aatctcttta aacaagcttt tgtctttgtt cttttctcga tgttttctgt
    10561 aaaggagaac tgaaaaccaa caaaaaaaca aaaataataa gctacttgtt tgtgtaaaga
    10621 aattcttgtt caacgaatct ttaggtaacg aataaacaat aaagagatat gaaaagccgt
    10681 acaaagaagc attccccatt ttgggatcac tagcggttgc cattaatatg ctgcttagta
    10741 aaataaaatg aatcaacatg cccataactg accatttgat cagctggaca tacggtgcta
    10801 ctaatagtga gaacatgacg attgctaaac ctatgatcga atgggaaatc ttataaccaa
    10861 agtcaacaaa acgaatactc atcaacatac agaataaaac cacagcgtat ggtttatgct
    10921 tcgattcaaa taatgttgta attataatga taaaacaaat ggcaaacaat acgatgaata
    10981 atgatcgtaa aaacaatgaa caaatataca tgcacttttc ttttttagaa gctgcttctc
    11041 gtattttttg ttttaaaata aacggatcta gttgtagaga ctgataaaaa tccacctgtt
    11101 gtcgtccttt ctttttactt ttatttttta cagtcatcta tgacaggttg taaatactat
    11161 ggaacatcat atggcgttgt cattttcttg tcaactattt ttcctattca tgtttttata
    11221 aaaaaacata tcgttttctt gtatgattaa atcaccacaa aataaacaac tgagagaacg
    11281 atatgcttta tgaatggttc tatcaaaaaa attctgagat caatagacaa aaatttgatg
    11341 attacagagg tctcttacga aaccttacag aagaaaaaaa cattgatcgt tgctgcagtt
    11401 ctcttccctg ctccatgtgt ctgtagaaat tgtggttctg ctgtggtaga tggaaatagg
    11461 aaattaatgg tggtgaaaaa caagaagaag gaaaccattg tccactttga ataatataat
    11521 catatgccaa tggttatacg cctaaaaaag cagcgctata cctgtaaaaa ctgtcgaagc
    11581 cattggaacg ctcaaagtta ttttatccgt ccaaggcact cgatttcaaa tcatgtcaga
    11641 cataaaatca cttctttact gactgaaaaa gtgtcgttat tttttatttc gaaaagttgt
    11701 taggtatctt tgacaaccgt tattcgcaca ttgaaagagt tgagaagcta tttaccaaag
    11761 tcttccaaaa gaattctgtc ttgagtgttg agggttgtga atttcgttct cacgtttccg
    11821 ttgaggataa actgagtttt atttccgcgg atggcgagac agaaaaatta atcgagaaaa
    11881 tttacctaca caaaaaattc ctcgattaac aaactatttc cttagctgca ctaatcctaa
    11941 agaaatagaa ttcttagtta cagacatgaa cgccacctac tttcagctca ccaaacatgt
    12001 tctaccaaat gcgaaattgg tgattgatcg attacttggt ttttccacgc ctttcaagga
    12061 ggcgtaacct ttattcacga gttggttgaa gctttccgag acaaaagacc cgacttgttt
    12121 ttctccttat tgacaaagag ccattaaaac tagagaaaaa attacaactg atcgctgatt
    12181 atttgtagaa agaaaatcaa agtattaccc aataaacttg agtcattagg caaaaaacac
    12241 tgcgaaactc aagaatgatt ttcgcagtgt ttttgtatat tttttattaa ctagtattac
    12301 tacaaaggtt tatttttcac ttctcaacgc agcacaaaat tgtctttact tatttactgg
    12361 ttgggttcaa ccttctctac tccgatacta atatttgttt gtatacccct tctaaaatcg
    12421 gtagtttcca ttcaattgat gggtactagt tgattttaaa aactatgctt attcaatttt
    12481 ttattttagc tcatatattt cgtttctttc taaattttat actgatgaaa acaagtagca
    12541 cacccaatat tgacaatata aactggctaa tgatggtttc tccagttttt ggaagatgct
    12601 tatttccact gcttgttgga ttgtttcctc ttatatctga gctatcagca acttttctat
    12661 tctcttttgt taaaaagatc gccacttctt gttctttttc gatcttgaaa gttactggtg
    12721 tcgcatccaa ttcatacccc gttggcgctt gagtttctac cagctgatat gctcctg
//
LOCUS       DBNICI010000077        12736 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.077, whole genome shotgun sequence.
ACCESSION   DBNICI010000077 DBNICI010000000
VERSION     DBNICI010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.077"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(87..266)
                     /gene="rpmF"
                     /locus_tag="AC1ZCC_001721"
     CDS             complement(87..266)
                     /gene="rpmF"
                     /locus_tag="AC1ZCC_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIQ1629969.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(324..893)
                     /locus_tag="AC1ZCC_001722"
     CDS             complement(324..893)
                     /locus_tag="AC1ZCC_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIQ1629970.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1079..1981
                     /locus_tag="AC1ZCC_001723"
     CDS             1079..1981
                     /locus_tag="AC1ZCC_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIQ1629971.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="AC1ZCC_001724"
     CDS             complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="AC1ZCC_001724"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIQ1629972.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="AC1ZCC_001725"
     CDS             complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="AC1ZCC_001725"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIQ1629973.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="AC1ZCC_001726"
     CDS             complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="AC1ZCC_001726"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIQ1629974.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8549..8734
                     /locus_tag="AC1ZCC_001727"
     CDS             8549..8734
                     /locus_tag="AC1ZCC_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HIQ1629975.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8796..9992)
                     /locus_tag="AC1ZCC_001728"
     CDS             complement(8796..9992)
                     /locus_tag="AC1ZCC_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HIQ1629976.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10162..11598)
                     /locus_tag="AC1ZCC_001729"
     CDS             complement(10162..11598)
                     /locus_tag="AC1ZCC_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HIQ1629977.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11891..12700
                     /locus_tag="AC1ZCC_001730"
     CDS             11891..12700
                     /locus_tag="AC1ZCC_001730"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIQ1629978.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2673 c         2250 g         4010 t
ORIGIN      
        1 atatttttta tttaccgact acaaaaaaat aaacctcaag gctccatggt tagccttgag
       61 gttttacatt ttctaattaa aatgtattaa gcttcttttg acattacgtc cttaccatca
      121 tagtaaccac aatttgcaca tacgtgatgg ctttttttca tttcaccaca gtttggacat
      181 tcgtttaacc ctttgattgt caatttgtag tgtgtacgac gttttgcttt tttagctttt
      241 gatgttcttc tagctggtac tgccattttg ctacacctcc ttgtaaagtc ttgtatctaa
      301 attagacaca atatattcca atcttacttg ttatcgtcgt cttttgattt agaatcaaaa
      361 agttcagata gttttgcaag acgaggatca attttattct cggcttcttc catttttctg
      421 cgctggtatt cttcttcgga aacaacttcc caatcattgc cttttggaag ttctgttgct
      481 gcttcctctt cttttgtcaa tacccgcatt ggaatcgcca ataagatgtt gtcttcaatc
      541 gactctgcta aattcaataa tggaccttca attagcaaaa tctcatctgc agacatttgt
      601 tcatctctac tttggaattg ttcttctgtc atgaatactt cgtctacaga aaaatccatg
      661 ggcagttcaa caggttctag tgaacgtgtt gaaggaactg tcaaagtcgt ctgcacagtg
      721 taatgaacga tatagccttt cttatcaact gagaccaaac cttcgacttc cactggcgca
      781 acatcaagta ttaaattgtc tcgcttcatg agagcggctt tcaagtcgag cgtctcatga
      841 aaagttaacg gtgtctcctg gtattttctc aattccaata aagaccattt catttttcat
      901 cactcctaag caacaaaagc aattatacag ggcaaaaagc atgttgtcaa tgaaattttc
      961 ttgacacctt gcagttcata aaaaaacaga aaaatcttta ttagaaagcc tctgtttgct
     1021 atcatcctca gaatcattta caataatcat tattccatta aaggagcgga tgatcttcat
     1081 gaacacgaaa gatatcgaat actatgtcca aatcgtcaaa caaaaaaatt ttacgaaagt
     1141 ggctaattat ttccgtgtga gccaacctac tattacatat gcattacaac gtcttgaaaa
     1201 agagtttgac accgtactta ttacaagaga tcgatcgcat cacgaattaa tcgtcacgcc
     1261 ttctggaaag cagctgtttc tccatgcaca aaccattctc caagagattt ctactgcaag
     1321 agctgaaatc actcagttgc aaaagaaaaa gcttcgtttt ggcatgcctc ctattatcgg
     1381 caatgaatac tttcctaaac tgtcaagtta ctttatcaag catgaattga tgaatcaaat
     1441 tgagattacc gatggaggct ctcgggactt atacgggatg atccgacaag ggaggatcga
     1501 tttagctgta ttaggctcaa cacagccaat cgtggatcaa tatttagaaa cagagttact
     1561 tttggacaaa cgtttcatga tcgtcgtgcc acctacacat cctctttcca agcaggatac
     1621 tgtctctttc aaagatatag cagatgaaca gtttgtacta ttgaacgaac attatgtcca
     1681 ccccgcagca ttcactaagc tttctaaaca aacacatact catccaaaag ttgtttatca
     1741 aagcaatgac ttgacgattt taaagagtat ggtccgagaa aatgtgggta tcggtttttt
     1801 ggcagagatt gctgtccatc ctgaagatca tttagtttgc ctccctcttt cagatgaagt
     1861 tcagcctcgc ttcttgattt cattggcaaa acgaacacag cagatcgtca cgcctcttca
     1921 agaacaagta gtccaagtta tccatgaatt cgttacagca caaaaaaaca gctcttcgta
     1981 aaagaagagc tgtttttatt ttttattaag ctaaattaga tagctgttgt ttcgccacgg
     2041 taaacgatgc cgcgacgtga atcaacggta atcaattcac cttctgtaat tgttgttgtt
     2101 gcgcctttag caccaacgat tacaggaata tcttgtgcaa ttcctacaac tgcagcgtga
     2161 gaagtcaatc cgccttcttc aacgataaca gcagctgctt tttcgattgc aggcatatat
     2221 tctttgtcag ttgatggtac aactagaacc attccgtctt ttgctttttt tacagcttct
     2281 tcagcagatg aagcaataac tgcatttgca acgacagatt tttgtccaac gccttgagct
     2341 tctaataatt tagaaccaat catttgaatt ttcatcacgt tagttgttcc gcgttcacca
     2401 actggtacac cagcagtgat caagattaaa tcgccttctg atgcgaaacc taattcaaca
     2461 gcttttttag ctgctagttc aaacatttca tcagttgttg atggtttttc agcaactgtt
     2521 gggtaaacgc cccagtttaa catcaaacca cgttttgtgc gttcgtcaaa agtaacagcc
     2581 aagatatctg catctggacg atattttgaa atcatttttg cagtgtatcc tgattcagtt
     2641 gcagcaacga ttgttttcac gcctagattt tttgcagcac gagctacaga taaaccgatt
     2701 gtttcagtta catctgtttt atcgaattcg ttcaattgga atgtacctaa ttctgataat
     2761 gcattttcag cttcttggtc gatacgtgcc attgttgaaa cagcttcaac tgggtagtcc
     2821 ccgtttgcag attcaccaga tagcattgtt gcatctgtgc catcaaatac agcgttagca
     2881 acgtcagaag cttccgcacg tgttggacgt gggttttctt gcattgattc taacatttgt
     2941 gtagcagtga taactgggat accagcagca ttacattttt tgataatgcg tttttgaacc
     3001 attggtacta gttcagctgg gatttcaaca cccatatctc cgcgggcaat cataacaccg
     3061 tcagaaactt tgatgatttc gtcaatgttg tcgatacctt cttgagattc gattttaggg
     3121 aagatttgaa catgagtcat gtctttttct tctaagattt cgcggatatc tagaacgtct
     3181 tgtgctttac gtacaaaact tgcagcgata aagtcgatat cgttgtctaa accaaaacga
     3241 atatcgtcag catcttttgg agtaatacct ggcaaactaa tagaaacgcc tggtgcattt
     3301 acccctttac gtgatcctag catacctgca tttttaactt ctacgactaa ttcacggttt
     3361 gcttcgtctt tttcaatgat ttgcatatcg atcaaaccat catcaaataa aatgtgtccg
     3421 ccaacgtgaa catcgtcaaa taatcctggg taagtaaccg cgattttttc ttttgaacct
     3481 acatgttcag gatccataga gatgcgtgtt tgatctccta cttcaaattt gatgtatcct
     3541 gcacgaccaa aatcagcttc tgttgtgcct tgaacagttg tacggatttc agctcctttt
     3601 gtatctaaaa gaataccgac atcttttcct gtctttttaa ctgcttcacg gaccatagtc
     3661 atacgtgata attgttcttc atggtcacca tgagaaaaat taaagcggaa gacatttgcc
     3721 ccagattcaa tcaattgaga aataatttct actgagttac ttgctggtcc taatgtacta
     3781 acgattttgg tttttttcat ttaaaaaact ctcccattca attttgttta tttaaagctg
     3841 tacacagctt aaatagcttg gtttctttca ctcttaaaat gaaagtgaat tatttagatc
     3901 atatagtgat aagtctggtt tatgtttgtt gttttcaagt gtatcaatga tgtcagacgc
     3961 aacgatttcg ttgtcatgga taccgacaca taatccacct tttccttctt gaagcaattc
     4021 gacagcgtaa gcaccgaatt tacttgccaa cacgcggtcg cgtgcgcttg gtgctccacc
     4081 acggacaacg tgtcccaaga tagatacacg tgtatgatag tcaccaaatt ctgaaagttt
     4141 ctcagcgaat tcatttccgc ccattacacc ttcagccaag atgatcaagc aatgtttctt
     4201 cccgcgatcg cggccttctt ggattttttt agcaactgaa gccatatcaa aatcatgttc
     4261 tgggatgatg atttcatccg ctccgccagc cacacctgac cataaagcga tgtctcctgc
     4321 attgcgtccc attacttcga taacgaatgt acgaacatga gaagtcgctg tgtcacgaat
     4381 acggtcaatt gattctagta ctgtattgat tgctgtatca aaaccgattg tgaaatcagt
     4441 cccaggaata tcattatcga ttgttccagg aatacctact gctgggaaac cgcgttttgt
     4501 caaagccatt gcgccatgat aagagccgtc tcctccgata accactagac cttcgattcc
     4561 gaattttttc aattgttcga ttccttttaa ttgaccttct tcagttgcaa attctggata
     4621 acgtgcagaa tacaagaatg taccaccacg ttggatcttg tcacctacat ctgccacatc
     4681 aagacgacga atatctccag caactaaacc ggcaaaacca taattgatcc cgtatacttc
     4741 cagtccttcg taaatacctt tacgaacaac ggcacgaaca gctgcattca ttccaggtgc
     4801 gtctccgcca ctggtcaaaa ttccgatgcg tttcataata aacattcacc tcattaaaat
     4861 actttatcct aagtatgact taggtttaca accatttttg cgttaaccac gactaacatt
     4921 ctaacattat ttttctcact tgtcttgatt tacggataaa aaaatgaaaa aaaatgaaag
     4981 caagtagtat tttttaccgt aagttatttg aaaaccacat tatcttcgcc aagcagataa
     5041 ataagttgcc cctttatact cttgctgtca tccacccaat aagcatctga cagaactact
     5101 tttcgttgcg ttttttcgtg atacataact actggtatat atcctgggtg tttctgaaaa
     5161 atcacttgta attgtttcat tatctcggga tcttcccgtt cttctgtaat acgcaaatag
     5221 caagtttgtg aaggatactg ggcttctaac tggttaggat cagcaaagct ttctccaatt
     5281 agttgcagtt cctgattata tttgctgatt tcgctccgcc cttgaacaaa gatcactcga
     5341 tcaacggcaa acaaattacg atatttacga taaagagttg gaaaaactgt gacagaaatt
     5401 tctccactac tgtcggttcc ttcaacaaag gccatcagtt ctcctttttt tgtccgaatt
     5461 tctctaactt ccttgatcat cactaataaa ttgactggtt gattagggac gatatctgta
     5521 atcagctgaa cttgtttagc taaacgaaga cgatcaaacc cttctgcagg atgacctgac
     5581 aaataaatac ctaagtactc ctcttccagc ttcaagcgtt cttctagtgt atagtcagcg
     5641 acttcttctt cttttaatgc catgattccc agcaagtcaa gacttccacc actgtaaacg
     5701 atattttgga tttttccttc gagatcaagc atcaattgtt tcctgttagg agataattca
     5761 tcaaaaactc ctactgctgt cagtgattgc agtaactcta atttcagcca acgcttatcg
     5821 atccgtatga ggaattgatc cactgactgg aacgggccat tttgttttct ttcgtttaat
     5881 atatcttcta caaaatcacg ccgaatacct tttatcgctg tcagtccgaa tctgatttct
     5941 tccttattga ccaactcaaa actggaatta ctttgattaa tggacggcga ggaaagtttg
     6001 agtttatttt tacgtgcttc tgcaatatat tctttcaatt ttgatgtgtt atgccgaaca
     6061 gaattcatca ggctggtaaa gaaagcacct ggatgatggg cttttagata cgccatttgg
     6121 aatcccacga atgaataagc aaatgcatgt gaccgattga atccatagtt tgcaaatttt
     6181 tcgatatatt catatacggt cgctgctgtt ttttccgaat atccctgttg taatgctcct
     6241 gatacaaaat gcgacttttc ttcatctaat acgtcttttt tctttttact cactgcccgt
     6301 cgcagaatat ctgactgccc taatgtaaat ccagccatag tcgaggcgat ctgcatgatt
     6361 tgttcctgat agacgatcac tccatatgtg tttttcaaaa tcgggatcag tgaatcatct
     6421 gggtagcgaa tggcttcttt tcctttcttc ctggcaatga aggtatctat attttgcata
     6481 gggcctggtc gatacaatgc attgactgcg gcaatatctt cgatgttttc tggatctaat
     6541 cttcgtaaaa cgttccggat accagcagat tcaaattgaa atactcctga ggtttcgcct
     6601 cgttgaaaca agagcagcgt ttttgcatca tttagcggga tctcttttaa tcggatctca
     6661 tacccttcta ctcgcttaac agctttcaaa gtaaaatcaa taatcgataa gttgcgtagt
     6721 cctaaaaagt ccattttcaa aagaccgatt gcttctacct cattcatcgt atactgggtc
     6781 agaaaagctt catcagaacc gcgttggagc ggaactagtt caagtagatt cttatcgctg
     6841 ataactacac ctgctgcgtg agtcgaaaca tgtcttggca atccttctaa aatcactgca
     6901 gttttgaata aaagttggtt tttttcgctt gcctgaacta attctactaa ttgatgcgaa
     6961 gttttataag cttgggtcaa tgtgattttc aattgattgg gtattgcttt tgaccagcgg
     7021 tttgcttcac tttgcgataa accaaacact ctagctgcat ctctaagaac catttttgct
     7081 gccattgtgc caaatgttgc gatttgtgcc atatgatatt gtccatactt ttctcgaaca
     7141 taattcaata cttcttcacg tcgattgtca ggaatatcca aatcgatatc tggcatcgta
     7201 aagcgttcgg ggtttaaaaa gcgttcgaac agcaaatcat attctaatgg atccacatct
     7261 gtgatttcta acacataggc tactaatgaa cccgcagcag aaccacgacc tgcaccggta
     7321 acgattttgt ttctatgagc aaaagccatt acatcccata cgatcaagaa ataatcatcg
     7381 aatcccattt gatggatgac agataactcg tagtccaaac gttcttggta ttcctttgta
     7441 tagtcagtga ttcggcgttc cacttgcgaa tagcataact ctcttaaata ttctccagct
     7501 tgttttccat taggaactgg ataatgcgga agtaatttct gatggaactg gaattcaaat
     7561 gaaatcgatt cagctaaacg ctgggcgcct attacagctt gggaaagacc cgctttttcg
     7621 aatcgtttga tcatttcttg ttctgaaaga agatagacat ttgctgttag tggtaaagat
     7681 gcagataaat tttctaaagg tatttggctt ccctctttga tatgtttcat cacttcaagt
     7741 gcaaaaagtt cttcttgttg taaactagcg atgggatgta atgcacaaag cgaataattt
     7801 tcttttatca catactgata ccattcaggc aattgatcag gcgtgttttc tgtcaagccg
     7861 atataaaaat ttctagtaaa aacattttca agtaaaaact gccaatgacc gaaaaagtta
     7921 tcctctttat tgaaagcctc agaaacagaa tgatttgccg gaacaacaaa cgctagatgg
     7981 tcggtttctg tcatttctgc aagttccaat ttttcttgga tcatttttcg gctggaaagt
     8041 ttcatcagct gctgataacc tatttcatct tttgcgtacg caagcaagcc ataatttttt
     8101 tcttcataag tataactaag atgtaatcca atgattggct gtactccttc ttctacacaa
     8161 atacgataga attcaggtgc accgtataag acatctttat ccgttatggc taatgaagtg
     8221 tagccttgtt tttttgcttc ttgtacatat tctcgaatac gtatcgtact ttgtaaaaga
     8281 gaataagaag taattgtata aagttgtggg aaaaacatct tgcattccgc cttttttttc
     8341 gttaaaattc actagcgtta aaaggtttga tccttttact ttcattaaga tagcaggcgc
     8401 tgaagttgat tattccactt catttgcttg cttgtaatct gtaggaatga gttctttcgc
     8461 aagaactcaa attttattga gatttggtct cgtttcttta cattcaaaga aaatctggta
     8521 aaattaagat agaaaagagg tgttctttat gcgatatatc gttacattat tctgggctgt
     8581 cgttctaggt caagtagtcg gttatattgg tgctgcatta acaagcggaa catatgattt
     8641 tactttgaca acaatcattt cattcattgc gggtgtcatt atccttttga ttggtgcagt
     8701 tgcgccaaga aaagaaacct ctgcccattc gtaaaaaaac agccaagagc ttgagatttc
     8761 tcaagctctt ttttattttc tactcctcac caccattata atctgctgcg aagaacttta
     8821 cgaaagttga ataaagaaag aagaatattt gacagaacaa acaaacttgt cacaataaaa
     8881 acagcactgt aattataagc atgcgcgact gtcgatccta gcatgggacc taaaacttgt
     8941 ccaaagttgc taaacatctg attgtagcta tagattcggc tgactccttc ttgaggcgta
     9001 atcttactga tcagtgtatt gacagaaggc atcaaagcgc cagttgaaaa gcctaaaata
     9061 aagcgaagta tacctaattg gagcggtgta gtgacgaatg ccataggtaa ataacagcaa
     9121 aatgataaaa tgagtcctcc aattagaact ttttgattcc caatcttatc ccctagtttt
     9181 cctagtttcg gtgaagaaaa gacagcggaa acaccagcta tcgaaacaat caagccgctg
     9241 acaaaaagga tattttctgt acttccgctc aattcacgaa tatataaggt caaaataggg
     9301 ctgatcgtag tgattcctat ctgtaaaatc aaggtcgtaa tgaagagacc gatcaaaata
     9361 gagagatgat ccatttttga aaaaatttct ttggtgctta tcagatcttt tttctcgatt
     9421 ggatggaaat cttctttgac taagaaaatg gtcaataccg ttgtgatcat caacaaagcc
     9481 cctgtgatca agaatacatt ttccatacca aaccactgag ccagtgcacc gcccattgaa
     9541 ggtccgatca gatttccagc aatcgctccg gtagacaatg ttcctaacgc ccatccgctc
     9601 ttttctctag gtgcctgtga agcaatcatc gctgtggcat ttggaatata tccagacaaa
     9661 atcccattcc aaaaccgcat gatcaaaagc caatacgcgt ttggtacaaa agccaaagat
     9721 cccattgtta atgtcatccc tgctgctgca cggatcatca tgagttttcg gccttttcga
     9781 tcagctagat ttccccagat tggtgcaacc acggcagatg caaaagcagt cacagagatt
     9841 gctagtcccg aaaacaattc gatttgatct tttggtgttc ctagttgctc aacataaact
     9901 ggtatgaatg gcataactaa actaatactt gcaccagtga aaaaacagcc aatccacgca
     9961 atcactaaat tttttctcca attaatctcc atctatcatc cctctttcgt ctcttttatc
    10021 atacctattt tcgaataaac aagcaacgat ctcgccagta aaaagagaaa aatcccgcaa
    10081 aggtcatttt atatgtaacg ttctttttat gtaaaacaaa aagagatcag aacaaatact
    10141 gttccaatcc cttctcttta gttaaattgt tgctagaatg atgaagttga taatgaataa
    10201 aatatcaacg atccacaaag caacagaaac ttgattgtat tttcctttga ttactttcac
    10261 aattgtatag aaaataaatc ctgctgcaat cccataggaa atgctatagc acaatcccat
    10321 aaagatcgat gcaaagaagg ccggtaaagc ttcttccata tctagccatt tgatgtctgc
    10381 aaaagaagcc atcatcatta ctccaactaa aatcaatgct ggcgcagttg cttgatttgg
    10441 tacaatggcg atcagtggag agaataaact acttaatgcg aatagcactg ccactactac
    10501 tgaagtcaaa ccagtacgtc caccggcccc gatcccagct gcagattcga cataagttgt
    10561 tgtattcgat gtaccaaaga tagcccccac agaagtagcg atcgcatcgg caaatagtgc
    10621 tttatccatt tttgttttga acccacgacc atcttccaac gcgatctcgt cttcttttga
    10681 gaaaatacct gtgcggcgtc ctgtaccgat aaatgttccg atcgtatcga aagtatcaga
    10741 taaactgaat gcgatgatcg tcatcaatac ttgaggaatc ttggaagaat cactgaataa
    10801 agattgcatg ccttcagaac caaatgctgc accgaaagtt gttcctaatt ctttaaatga
    10861 attacctaac gaattcgttt gccaatcaat ggaacttaaa tcaacaacac ccataaaaat
    10921 accaaggact gtggtcgcta cgataccgat aagaatagcc ccacggacat tttttacaac
    10981 taaaatggct gtcagtacca aaccaatcac agccaataag ataggagcgt tgtcaaagtt
    11041 ggctaacgca gggacgattc caccatttat cgtaacattt gtcgcttttc cgccttcaac
    11101 gacagagctc gaaatagctg actggtctgc tgaaaaagat aagaatcctg catttttgat
    11161 tccgacatac gcaacgaaaa taccgatccc gccaccaatc gcatgttgaa gactttcggg
    11221 aatcgcccga ataatcattt ttcgaatatt tgtgacagta ataaaaatat tgattaatcc
    11281 gcaaataaac accattgcca aggcttgttg ccaagaataa ccaagcccaa aaacgaccgt
    11341 aaaagtgaaa aatgcgttca atcccatacc aggtgcctga gcatatggta catttgcgaa
    11401 caatcccatt accagcgtac caataatcga tgcaatgatt gtcgctagaa atactgcttg
    11461 gaagggcata cctgatgctg acaaaatcgt cggatttacg aataagatat aactcatggc
    11521 aaagaatgtc gttactcccg ccattatttc agttgataca tttgtattgt ttttctttaa
    11581 accgaaaaac ttttccataa aaagttccac tccctgaatt tttttctgat ggaaaactat
    11641 aataataaaa aataagtaaa atagggcaaa tccatcgaac aatttcaaat tatatagacg
    11701 tttcattcgc ttttcaacgt ttttttaaca aaaaaagcga aaataatact taacgttcgt
    11761 gtttagctta tgtttagcta tttatatagc cataactgta gtttttgtta ggaatagctg
    11821 attgctcttg ttaattacct aaaatatgct agactaaaac aaatgctagt tgaggaagga
    11881 aggaccctta ttgaataaaa aattaattgc catcgatctg gacggaacta ccctcaatca
    11941 agattcgatg attacatcta aaacaaaaga gactctaaaa aaagcaatca atgccggtca
    12001 tcatgtcagt attgctacag gacgcccatt ccgaatgagc catcagtttt accaacaatt
    12061 agaattaaca acaccgatgg ttaattttaa cggcgcatta gtacacatcc ctaatcagca
    12121 ctgggatggt gaaagagaaa cgttgatcaa tcgtgaaatc gcttttgaaa tcttatcgca
    12181 aaaaaagcag ctaaatttag attttatcgc tgctgaaaat cgtgatactt tttttattga
    12241 cagttttgat ttctttgatg aaaaaatttt tgcttcttct cgtccaggag agaagaattt
    12301 gctttctcct aaaaatttga cgacaaatcc tacatctttg cttgtccgaa cagacaaacg
    12361 ttttgcagga gctgtatctg ctgaattaac acgtcaattt ggcagttatg tcgatgttcg
    12421 gacatggggt ggacctactg ccatcttaga gattgtttca aaaggaatcc aaaaagcaaa
    12481 aggcgtgcaa gagattgcta actacctgtc tatcgatcaa aaagacgtca ttgcctttgg
    12541 tgacgagcat aatgatttgg aattgttgga ttatgcaggt tggggagtag ctatggctaa
    12601 cggtactgac caactcaaag gcattgcaaa tgatgtcact cctttgagca atcaggaaga
    12661 cggtttagct gtttatctgg aaaaactact taaactataa tagagaatta gattccacaa
    12721 aaaagaggtt gtgaca
//
LOCUS       DBNICI010000078        12704 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.078, whole genome shotgun sequence.
ACCESSION   DBNICI010000078 DBNICI010000000
VERSION     DBNICI010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.078"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(215..427)
                     /locus_tag="AC1ZCC_001731"
     CDS             complement(215..427)
                     /locus_tag="AC1ZCC_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1629979.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            complement(430..642)
                     /locus_tag="AC1ZCC_001732"
     CDS             complement(430..642)
                     /locus_tag="AC1ZCC_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1629980.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            917..1327
                     /locus_tag="AC1ZCC_001733"
     CDS             917..1327
                     /locus_tag="AC1ZCC_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1629981.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1340..1792
                     /locus_tag="AC1ZCC_001734"
     CDS             1340..1792
                     /locus_tag="AC1ZCC_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HIQ1629982.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            1883..3163
                     /locus_tag="AC1ZCC_001735"
     CDS             1883..3163
                     /locus_tag="AC1ZCC_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HIQ1629983.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIILLKKEKEDLNDEINQKYDETLKNVYSSLIVSN
                     IEETSSSEIKNKLSLLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            3235..4356
                     /locus_tag="AC1ZCC_001736"
     CDS             3235..4356
                     /locus_tag="AC1ZCC_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIQ1629984.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNGVS
                     LARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELI
                     SLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIK
                     NNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPL
                     KAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(4438..6210)
                     /locus_tag="AC1ZCC_001737"
     CDS             complement(4438..6210)
                     /locus_tag="AC1ZCC_001737"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1629985.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEQNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(6213..7925)
                     /locus_tag="AC1ZCC_001738"
     CDS             complement(6213..7925)
                     /locus_tag="AC1ZCC_001738"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1629986.1"
                     /translation="MKLILRHAKKYKVSVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8040..8708
                     /locus_tag="AC1ZCC_001739"
     CDS             8040..8708
                     /locus_tag="AC1ZCC_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HIQ1629987.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            complement(8775..9563)
                     /locus_tag="AC1ZCC_001740"
     CDS             complement(8775..9563)
                     /locus_tag="AC1ZCC_001740"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIQ1629988.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(9646..11118)
                     /locus_tag="AC1ZCC_001741"
     CDS             complement(9646..11118)
                     /locus_tag="AC1ZCC_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HIQ1629989.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            complement(11248..12441)
                     /gene="metK"
                     /locus_tag="AC1ZCC_001742"
     CDS             complement(11248..12441)
                     /gene="metK"
                     /locus_tag="AC1ZCC_001742"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIQ1629990.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     regulatory      complement(12439..12518)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
BASE COUNT         4310 a         2434 c         2032 g         3928 t
ORIGIN      
        1 cccttcttct gtttcaataa ttaatcttgt gtaattgttc tcattcaaaa tccctatttt
       61 tgattcttcc ttcatgtcat ccctccttcg tctctcgccg tcaccacctc caagaagagg
      121 agttgccaaa atgaaaaaac gtggactttg gcaaacttga aaggagagca gcaactcctc
      181 ttttcagaag tggtgaattt agatttttta gctattaaca aacagctcta actctaaatc
      241 gatttttaga acgccatgtt ctaaactgtt catataaatc gacatcaatt gttatttctt
      301 tggaagtaac tgcgttatac ccctcactaa aacgtgattt tttaaattcc ttcattcttc
      361 tttgataagt actttcagaa tacttatatt tctctttaaa ctccgctttg ctcaaacgtt
      421 gtaccatttt tacgcctcct gttctgatgg ctgatcatta gaatctactt ctaacttgat
      481 atccaaaagt tcttcgattt gttttaaacg ttccttaggt tttctttttt cgatgaagat
      541 atctcgtaaa taagtaattg atatacccat tcgtcgagct aactctcttt gactccaacc
      601 tttctttact aaagcaatta atacttgctg ttttaaagtc atgcttcata cccccttatc
      661 gaatattttc taatattttt gtctaataag tttgacaaaa actgcaaata tgctattatg
      721 taagcataca aaatacagcg cacaaaaagc gagcttactg atgtctgggg agactattag
      781 tttattgctt ttagtctttg tatgccgtaa atatcttgct tacaaaacta gtataactgc
      841 aaattctcta tttgtaaata gataatttgc agaaaaatac tttttttgtc ggtattttga
      901 aaggaatgca tatcctatgc tattagaccg tataaaaatc ttattaaaag aacgttcaat
      961 gacgttggca gaacttgaaa gaaagttaaa tttcggaaat ggaagcatgt ctagatggaa
     1021 taaaagtact ccatctggag ataaaataca aaaagttgca gattacttta atgtatcaac
     1081 tgattatctt ttaggacgta ctgataaacg ccattattat gatttaacag aaaaagataa
     1141 acaagatatt gctttacaag ctgaaaaatt acttgaaggc ttgaattcag atgctgaaac
     1201 aaattattat ggtgaacctt tagacgatga agataaggaa aaattatacg atgccattca
     1261 acttgcactt gccttaacca aagttaaagc aaagaaaaaa ttcactccta aaaaatatcg
     1321 tcaataggtg ggctttggta tgcatttaaa caaatactgg atattagaag aaatagaaaa
     1381 attatttgta gagtttggat ctgatgcgat taatccatat aaaataatca aatattctga
     1441 tatagattta attgaaactg atttagggaa taaaacatta ggacaaacga tacacaataa
     1501 aagatgttat gtgatattaa ttaatcaaaa cctatcacct tctatgaaaa gatttgtatt
     1561 atggcatgaa atagcccacg ttagactcca taaaggtatt tgcacagcag cttttagagc
     1621 aaacaacctt agttgtatga tttatggtat agaagctgaa gctaatatgt ttgcaattga
     1681 aatgttaact agaacaatag atgaacaaga tttatctgat atgagtcagt tccaaattat
     1741 tgaatattta gggcttcctc ataatcttga acatttagta ttacaacatt aaaaataaaa
     1801 aagcccatgc tttcacatgg actctttcct catttctgag taaactctac aataattata
     1861 tcaaagaaat gaggagtttt gaatgttatt attgattgga cttattttat taattatagg
     1921 aatcactaaa ctgtcgaaag ctaacacaat aaaaaaagaa gcttatgaat tagaacaaaa
     1981 aaatgatgaa aaactaaata aattacatga tagagaaaga tttatattaa gtaaggaaga
     2041 aacactatca gatttaaata aacaaattac tatgttaaaa aatgatatta ttttattaaa
     2101 aaaagagaaa gaagatttaa atgatgaaat aaatcaaaag tatgatgaaa cattaaaaaa
     2161 tgtctattct tcacttatcg tttcaaatat agaagaaact tcatcttctg aaataaaaaa
     2221 taagctttct ttgttaaaat taaaagaaaa ggaatatatt attgacgatg ctgtttctgt
     2281 ccctcttatt ttaaataaac gagagaaaaa taatttaaag aaaaaaatgc tcattccatt
     2341 tgattcagaa gtgaaaggat tactcaataa actaactatt tctaatatag actcgactag
     2401 ggaaaaaatc ataaaaatat ttgataaaat taataattta ttcaagaatg acaatgctga
     2461 attaaggaag ggtcttttag agtttaaact tcaagaacta gaacttcatt actcttattt
     2521 agtgaaaatt accgaagaaa aagaacaaca aaaagcaata aaagaacaga tgatagaaga
     2581 ggaaaaagtt cgtagagaaa ttgatcgtga aaagaaacgt attgataaag aacaacgtca
     2641 attcaattct gaaatttcca aattaatcgc atatatgcaa aaagctaatg cagatgtgga
     2701 aaaagaatta tatgcaaata aaattcaaga actggaagaa aaattaaaag aattagaagt
     2761 tatacgtgaa aatgtattac aaagagaatt aaatactaga gccggttatg tgtatgtaat
     2821 tagtaatatt gggtcttttg gtgaagacat atataaaatt ggtatgacta gaagattaga
     2881 acctatggat agaataaaag aattgagtag tgcatcggtt ccatttgaat ttgacgttca
     2941 tgcgatgatt ttttcagagg acgctccgtc tttggaaact aaattgcata atcattttag
     3001 aaaacaagaa gttaataaaa ttaatcaaag aaaagaattt ttcaaagttt cactagacga
     3061 aatagagaaa gttgttttag aaaattataa tggaacagta acatttacaa aattagctaa
     3121 agctgaacaa tatagacgat ctttagaatt gtcaaaagat taaatatata aaaagccttc
     3181 gggctttttc ttttaacaat taataggaac gtatattcga aaggaataga aataatgtgg
     3241 ttagaaaaaa taaatgacaa aaaatttaag tacaccgaac gttatacgga tcccttaact
     3301 gaaaaaaaga gaaaagtgtc cgttacttta tctagcaatt cacgccaagc ttggaatcaa
     3361 gctaacttac ttttgaatga aaaaatcgca gaaaaaataa aacgaaatga agaattacca
     3421 ctaacttttg gagaattaaa aactaaatgg gatgaaaaat acaagccaac tgtaaaagaa
     3481 agttcctatc gaacaactca ggtttatcta tcattaattt caaaatatat aaaagatgat
     3541 gtacttgtaa aaaacgttaa tagtaatctt atacaagata tgctagatta ctactatttt
     3601 gaaaaaaatt tatcatataa ttatgtaata catttaaaaa cttttacagg aatgattttt
     3661 aaattcgcca accgtcgata tggattaaaa tataatcccg taaatggggt ttctttagca
     3721 agaaaaccta agacaaacga agaaataaaa aaagaaaaag atggttattt atcaaaagaa
     3781 gaagttcaaa tgattgctag taaacaaaaa agttcacatc aacagtcacg ttattcttta
     3841 attactaaat ttttgttttt aactggacta cgttatggtg aattgatatc tttgactgaa
     3901 tctgattatg atggtaataa aattacggtt tctggtactt atgattacga gttaaaaatt
     3961 aaaacaacaa ctaaaaatac aggttcttat agaaccattg aattatcaag taatgctaaa
     4021 gaaatcatag atcaattaat tgaagaaaac aaattaatta agaataataa agataaatac
     4081 attttcatct ctaaaaatgg taacccgata tctattcaag catataatca atctttaaga
     4141 gcagtatcca aagaattaaa cctagataaa aaagtttctt ctcacatgtt aaggcattcc
     4201 cacatttctc tacttacaga attaggaatc ccattaaaag ccattatgga tcgtgtagga
     4261 catgaagatt caaaaacaac attaaagatt tatactcata caactaaaaa tatgcaaaat
     4321 caactagtag aaaaactagg aaaaatagaa atttaaattg ccccttttct gccccttata
     4381 aaaacaaaca acataaaata tataaaaaga accccgttat aacgaggttc ttttaattta
     4441 actttcaaat acaaattgat tatgataaag ttccgcataa aatccatttt ctgctaataa
     4501 ctcatgatga ctgccttctt caatgacttc cccatcacgc aagacaacga ttctatctgc
     4561 attcaagatt gttttcagcc ggtgagcaat cacaaagctg gttcttcctt tgattgcttc
     4621 atccattgct ttttggatcc ttgcttctgt taccgtgtct acattacttg ttgcttcatc
     4681 caaaatcaat aaagatggat ttgtgatgat cgttcgagca atactgatca actgcttttg
     4741 acctgtactg aataagttat tttcttcgga tatctctgta tcataacctt tttctaacgt
     4801 catgatgaag tcatgaatat tcgcttgttt tgctgcattg atgacttctt catctgaagc
     4861 gtccggtttg ccgaatgtga tattgtcttt gatcgttccg gagaacaata cagattcttg
     4921 caaaacgatt cctacatgtc tacgtaatgc atcaagatca taatctcgga tatctattcc
     4981 atcgatgact actgaaccat cattgacatc gtaaaaacgg tttagcagat tcatgatcgt
     5041 cgttttcccc gatcctgttg gaccaacaag agcaaccatt tctcctttgt ccacattgat
     5101 cgagacatct ttcaagacta gtttttctgg agaataacca aaatctacgt gctgcaacgc
     5161 aagacctttt tcaacaccat caaatgcttt accgttttct ggtttgactt catctttttc
     5221 atcaaatatt tcattcaatc gtcttgcccc tgtaatcgct aattggatca tgctgtatcc
     5281 agatgaaatc tgcatcagtg gttggtaata ttgttgtgaa tactggacga atgtcacaac
     5341 taagcctaat gccaccgaac gttccaaatc accatttaat gctaaccagc cgccgaaaaa
     5401 gataactatc gctgtattga ccagtgacat tccttgcatc atcggaaata gtagaccaga
     5461 ataagcttgt cctttgaagg ttgcttcgcg aacactttga ttttgctcta agaatccttc
     5521 aatcgtttct tcttgtaagc catttgtgat gatgacacgt tgtccgctga ttttttcatc
     5581 catataacca tttagtttcc ccacttcatc ttgctgaaga tccacatatt tccgtgcttt
     5641 gcggataatc aagacagcta tcaaaacggc tactggagtg gaagcaattg tagcccaagc
     5701 taactctaca ttttggcgga acatcatgat caagatcccg acaagtaaaa cagcattcgt
     5761 gatgacttga agcaaagctt ggttcaaact attttgaata ttatctaagt cactggtaaa
     5821 acgacttaaa atttcaccgt cttgatgaga atcaaagaaa cggatcgtca atttttccaa
     5881 tttgttgaat agaccgatac gcattcggtt cgttgacttc cctactactt gtgtgaacaa
     5941 aatactatag ataaagttgg ctgcactagc tagtacataa aacagcaaga gtttccatat
     6001 tacagaaaca aatttgcttt tatcgtctac gccttgcatc aagcctccta cgtaattagc
     6061 taattcttga aaagcttccc cgatgtattg aggtgccttt acttgtagat aggtagcaat
     6121 gatcactgtg ataaagataa agaaaaagga gagtttgtac cgcttcaaat agtgatagaa
     6181 aaatttactt gcttttatta gatcagtcat acttactcct ccgttccttt ttgagtttca
     6241 aagatttcct gatagacacg gtttccagct accaattcct catgggttcc ttcgccgact
     6301 aatctgcctt catccaatac taagatgcgg tctgctttga caacagaagc aattttttgt
     6361 gcgatgacaa tcgtcgttgt tcccttcaaa tctcgatcga gtgcttctcg gaccagcttt
     6421 tcagagcgag catccagtgc actcgtactg tcatctagga tcaagatctt tggattaccg
     6481 ataacgccac gagtaatgga taaacgttgc ttttgaccac cagaaaagtt actgctacgt
     6541 tcttctattg gtgcttcgta acgatcagat aacttttcga tgaattcttt tgcttgagca
     6601 atagaagaag catgttccat ctctttttct gttgcattct ttttcccttg gcgcaagttt
     6661 tgagcaatcg ttcctgagaa taaaattgct ttttgcaaaa caaaggaaac cgtttgtctt
     6721 aaactctttt catttacttg acgaagatct acaccaccta cttcaacttt gccttctgtc
     6781 ggatcaaata gacgagggat caattgtgct agtgttgact tcccggcacc agtcgccccg
     6841 acgataccaa ccatttctcc aggtttgata gaaaagctga tatctttaag cgtatctgta
     6901 tcctcaccag gataacgaaa agaaacatgg tcaaaagtga cagaacccgt caactcttgt
     6961 tctggaacgt ctaaataagt aagatctggt tcagcatcta aaatttccgc aatccgcttc
     7021 agcgatacgg ctgctcttga agtcatcatc atcatcattc cgccaataat gattgccatc
     7081 ataatctgca tcaagtagtt catgaatgaa gcaattccac caattaacgt aggatcatct
     7141 ttaacaagat cgctcacaaa aaagatcgcc ccaaccacag ccaaattggc agccagcatg
     7201 aaggacggga tcatcacaga aaataatgta ccgactatga tgttgtgttt tgttaagtcc
     7261 tcacttactt tgctgaatga cttcaattgg ttttcttctt gtacaaaaga tttcactaca
     7321 cgaatcccta gtaagttttc ttttgcaatg ctattcacac gatcaatcag tttttgaatg
     7381 atcatgaagt gcttgcccat ccttgtaaat gaaagtgccg taatcagaaa taccgtaacg
     7441 ataagcgcta cgatgatcca ccataattgc ggcatcgtga tcatagctaa aatgaaggca
     7501 ccaataaata agaatggaat tcgaaaaagt gattgcaaag aaatcatcac taaattttgg
     7561 atttgcgtga tatcatttgt tagacgaaca acaagatttc cagcagagaa ctgttcgata
     7621 tttccaaatg aaaatgtctg gatctttcga aatgcttctt ctcggatatc tgcacttacg
     7681 ccttgagcta cttttgcaga gaaaatcgtg ttcgttacac ctgcggctaa tcctagcaaa
     7741 gccagactga tcaggtaaat accaattgtt ttcatttcat cattatcttc tgtaataata
     7801 gcttccaata cttgctgtag taacttaggt tgccataatg ctgctgcaac catgactgct
     7861 acggacagca aggaaataaa aacagatact ttatattttt tcgcatgtcg taagattaat
     7921 ttcattcgtt gattacaccc ctttgtttaa attgacagga ctaatttaac actgtaaaat
     7981 tagaccgact aacaatttac catgtttaaa aagataatgc aatacacgag gttgataaaa
     8041 tggaaataaa gctttctcaa gcaatcatca ttcaggccgc ctttgatatc ctggctgaaa
     8101 cacaagctct ttcgcagctt tccatgcgaa acatcgcaaa cagaattcat gttcaagcac
     8161 ctgctttata ttggtatttc aaaaacaaac aacagctctt gcaaaaaatg gctgaaacaa
     8221 tggaagaaga attagcgctt ccagatccaa ctttgccttg gaatgaacag ttgctgcagt
     8281 atatggaaaa ttactatgac ctttatacac gtttcccttg tggggcagaa ttagaaatca
     8341 atactgtgcc cgcttttccg agtcgattag aacatttgga gcaaatgatc caattacttg
     8401 ttcgtgaagg attttcagtc acccaaagtc atcaggccat tgcctctctc cacaatttat
     8461 tgattggaca gttaatggat cagcagcatg aacagcagct tcgacaaaaa attatggacg
     8521 gaaatgaatt tttgaaagaa acagttctat ttatgcgtaa ttatgtagtt gaaaatcaat
     8581 tagagggatt taaagaaagt atgcagtccc atgcagaatt gaatgaaaaa gcactctttc
     8641 taacgagtgc acaaacctat attgaaggct tatgccaaca aaataaaaga agagaaaacg
     8701 agcagtaaaa aagctcctat agagatcact atgtttgtga tctctatagg agctatcttt
     8761 ttaagactct ttttttattt ttcttccatt agccacgcca atgcacgttg aatccattgg
     8821 tcccagaaga tccagttatg atcgccaggt ccttgttcaa aggtgaccgc caagccttgt
     8881 tcctgcatag cagataccat ttcctgattt gcctgataaa gaaaatcaga ttccccacaa
     8941 gccatataaa atcttggaac tggttttccg ctttttacta gttctttcaa taagtattgc
     9001 ggatcatttt ttgaaccttc gatttgatcc aatggtccaa agatcccttc ccaaaaagat
     9061 gctttacgaa ccatcaaaag atcatccatt cgatttccta cagaaacagc gcctgaaaga
     9121 gagattactg ctgcgaaatt ttctgatttt gcaaggccta gttttaacgc gccatatccg
     9181 cccatcgata atccagcggc atatgttttt tctctttttc ttgttaattg cgggaataat
     9241 tcatgacaaa tctcaggtag ctcttctgca atgaatgtcc aatacttcat gtcataaact
     9301 gtatctgtgt accagccaag atcagtagat ggcatgatga cagctaatcc gtagtttgct
     9361 acataacgtt caatcgacgt acgtctttcc catacactat gattgccacc cattccatga
     9421 agtaaatata aaacaggtat atccgttgtc tcttgctctg aactggtgcc aatcaccttt
     9481 tttgttgttt gcggtagtat cacatttaga ttgacttcca tttctaacac attcgaataa
     9541 atatttgctt gaataaatgc catattttcg ccttccttag taagaagtta tttttgaata
     9601 actttaaatt tgaatgattc ggctacgtac atatcttaac cgaaactact gttttatttt
     9661 accacttttt tcatgaacga aaagcgttaa gaccaaagca atgatcacga gtaacaagcc
     9721 aactagataa acggaatgta acccatcata aagaattttt cttaatggta acaaaatatc
     9781 cgcatcaata tatttagccg tttgtggatt caccagttta ttcatgatat ctgcatcatt
     9841 tgttaaattt gctgctgcaa gttctcgttt agttacccca tttatcaata aaccaaaaat
     9901 agaaaccatg actgtttgac caattgttcg aactaacgta ttaaaagaag ttgctacacc
     9961 aagctggtca cgagatacac tattttgtgc actgacagtc gaggtagtca tggtcacacc
    10021 taagcctata ccgatgaccg ttgatatagc aaaaaaccat aaatagttac ttctgaaagg
    10081 cataagatac aaccaagcgc ccccaatagc tgcgattagt agtccaattc tcaagaccca
    10141 atgagttttc atttgagtca tccacttgct tgcaaggaaa gaaccgacca tccagactag
    10201 tgataatggt gctaatacca gcccaccaat tgctgcattc agccctaata caccttgcat
    10261 ccacataggg atataaacat cgattcccat caaaaaacca ctagccaatg ctgcagccaa
    10321 gttgaccaca acaaatagtg gatttttgaa taaatgcatc atgatgacag gatctttagc
    10381 tcttttttcg gcaaaaacaa aaatacctaa cattacgata ctacctagca gcaaagcaga
    10441 aacaagcgga ctcattcctt cgtctcctaa taattggaac cctaacagca atgagacaag
    10501 catggccatc aaactaaaac taccaaaaat atccattggt ttagactcat gtatgatttt
    10561 tggttcattt aaatatagcc aaatcaaaat aattaataga attccgatcg gcacattgat
    10621 aaaaaagatc cagtgccagc tgatcgtttc aacgatgaat cctcccgcaa gcggtccaaa
    10681 aacactggca atcccccagg ctgaactatt caaacctaaa acttttgctc gcttttcaat
    10741 agaataaaga tcgccgatga tcgtcaaagc tactggcatc attgccccag cacccatccc
    10801 ttgaatcgca cgagcagcaa tcaacccaat catgctttga gataaaccgc aaaataacga
    10861 accgataata aacaataatg tcccaaaaat aaagattggt ttccgtccca ctttatccgc
    10921 aagttttccg taaatcggag taagcatagc gtttgtcagc aaataaatcg aaaataccca
    10981 gttcatgatt tccattccat gtaatgaacc gacaatcgtt ggcatagcgg ttgttacgat
    11041 tgtaccttca atagccgtca tgaacgttgc aatgaaaata ctcactgtga ccaaagtaac
    11101 atttgtttct tttttcatct tattcctccc tttcattatg acaggatcta aaaatagata
    11161 aataattact atctttttca agaggttaac tgccataaaa aaagctggaa caaccaccac
    11221 ttggcgatcg ttccagctga atataactta ttttgctaaa cttgctttca acgcatctac
    11281 tttgtccgta tgttcccaag gcaaatctac atctgtacga ccaaaatgac cgtaagcagc
    11341 tgtttgtttg taaatcggac gacgcaaatc aagcatctcg atgattcctg ctggccgaag
    11401 atcgaagttt tctctcactg cagcaatcag ttcttcttcc ggaaccgttc cagtaccgaa
    11461 agtattgatt gagatcgaaa caggttgagc aacaccaatc gcataagcta gctgtacttc
    11521 tgctttgcga gcaagccctg ctgcaacaat gtttttagca atataacgtg cagcatagct
    11581 ggcagaacgg tcaacttttg tcgcatcttt accagaaaaa gcaccgccac catgacgagc
    11641 ataaccgcca tacgtatcaa caatgatttt tcttcctgtt aatccggcat ccccttgagg
    11701 accaccaatg acaaatcggc cagtcggatt gatataatat ttcgtttgat catctaataa
    11761 ttcagctgga ataacttcct tcactacttt ttcgatgaca tcatgacgaa ttgtttcatt
    11821 atctactgca tcatcatgct gtgttgaaat aacgattgta tctacgcgtt ccggttgtcc
    11881 ttgatcatca tattcaaccg ttacttgaga ttttgcatct ggacgtaaat acgttaattc
    11941 atttgattta cgcaaatctg ccaagcgtcg taccagacga tgactcaaag cgattggtaa
    12001 aggcatcaat tctggtgttt catctacagc aaacccaaac attaaacctt ggtcaccagc
    12061 accaatctca tctaatacgt cttttttatc ttcatcccgg atctcaagtg cttcatcaac
    12121 tccttgagca atatcaggag attgttcatc aatcgcaacc aatacagctg ctgtatctcc
    12181 atcaaatccg aattttgcac gtgtataacc aatttctttt attgtttccc gtacgacttt
    12241 ttgaatatct acatatgccg tcgtagatat ttctccaaat actaaaacaa gaccagttgt
    12301 tacagacgtt tcacacgcta ctcgtgctgt tggatcttgt tttaaaattg catccaagat
    12361 tgcatcactg atttggtcag caattttatc tggatgtcct tcagaaacag attctgatgt
    12421 aaataagtgt ctttctacca ttataatgat tccccctagt taaattcggt tacaaggcat
    12481 ctccgtcctg cggatccttt cgggaacttg caacgaacca agtatagcag ataaaacagt
    12541 tttctgctcg tattttttca tttttttgct taaaaaatat aaaaatattt tacattagaa
    12601 ctgtccaaaa aagcttagca caatcatcca taaagataac tctgctaagc tttttaaaat
    12661 cattctttca gataaaaaga aaaagatact caaaaagtat cttc
//
LOCUS       DBNICI010000079        12697 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.079, whole genome shotgun sequence.
ACCESSION   DBNICI010000079 DBNICI010000000
VERSION     DBNICI010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.079"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..1049)
                     /gene="fni"
                     /locus_tag="AC1ZCC_001743"
     CDS             complement(<1..1049)
                     /gene="fni"
                     /locus_tag="AC1ZCC_001743"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HIQ1629991.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEK"
     gene            complement(1046..2131)
                     /locus_tag="AC1ZCC_001744"
     CDS             complement(1046..2131)
                     /locus_tag="AC1ZCC_001744"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HIQ1629992.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(2144..3121)
                     /gene="mvaD"
                     /locus_tag="AC1ZCC_001745"
     CDS             complement(2144..3121)
                     /gene="mvaD"
                     /locus_tag="AC1ZCC_001745"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HIQ1629993.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(3114..4058)
                     /gene="mvk"
                     /locus_tag="AC1ZCC_001746"
     CDS             complement(3114..4058)
                     /gene="mvk"
                     /locus_tag="AC1ZCC_001746"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HIQ1629994.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            4374..5033
                     /locus_tag="AC1ZCC_001747"
     CDS             4374..5033
                     /locus_tag="AC1ZCC_001747"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HIQ1629995.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            5118..6023
                     /locus_tag="AC1ZCC_001748"
     CDS             5118..6023
                     /locus_tag="AC1ZCC_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HIQ1629996.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(6025..6657)
                     /locus_tag="AC1ZCC_001749"
     CDS             complement(6025..6657)
                     /locus_tag="AC1ZCC_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HIQ1629997.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    6808..6933
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            6977..7501
                     /gene="infC"
                     /locus_tag="AC1ZCC_001750"
     CDS             6977..7501
                     /gene="infC"
                     /locus_tag="AC1ZCC_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIQ1629998.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            7573..7773
                     /gene="rpmI"
                     /locus_tag="AC1ZCC_001751"
     CDS             7573..7773
                     /gene="rpmI"
                     /locus_tag="AC1ZCC_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIQ1629999.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            7826..8185
                     /gene="rplT"
                     /locus_tag="AC1ZCC_001752"
     CDS             7826..8185
                     /gene="rplT"
                     /locus_tag="AC1ZCC_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIQ1630000.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            8437..9774
                     /locus_tag="AC1ZCC_001753"
     CDS             8437..9774
                     /locus_tag="AC1ZCC_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HIQ1630001.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            9822..11951
                     /locus_tag="AC1ZCC_001754"
     CDS             9822..11951
                     /locus_tag="AC1ZCC_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIQ1630002.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            11926..12615
                     /locus_tag="AC1ZCC_001755"
     CDS             11926..12615
                     /locus_tag="AC1ZCC_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630003.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
BASE COUNT         3861 a         2416 c         2697 g         3723 t
ORIGIN      
        1 ctcttttctg tgcgtcttcc atacttcact aagtcgatcc ctcttgcttc gcaccaatcc
       61 ttcactggtc cggaaaagat cagagattgt tggtgtaggg ccgcggtatt cgttgcgccg
      121 accatagtat aaagcagacg aagctcttct tgccattgct tcatcagtat aatagtttct
      181 tctacgccgt gattcatcag atgcgtcagt acggttcctg aagtacccac ggcatttgcg
      241 ccgagacaaa gggctttaaa tatgtcatag gcattgcgga tacctccgga ggctaggatt
      301 tccatagaag tgtctgcttc atttgcttca agcaatgaag agactgtcga ttgcccccag
      361 tcagctaaat aactcagttc ccgcttggag cgacgggcgt tttcgatctg ggtgaagctt
      421 gttccgcttc gaccgctgat atcaactgta tgaactccta aagcagctag atcattgagc
      481 gtttctcttg tcatgccaaa accgacttct tttacgatga gggggacgtc aatggctgtt
      541 tgagtttctt gaataagtac tttccaattg gtaaaatcac ggtctccctc aggcataacc
      601 aactcttgag gtgcattcaa atgaatctgt aaggcatctg cgtgaaacaa tcggatagct
      661 tctttggctc tttcgacaga agtccctgca ccgatgttgg caatgatttt cccgtgagga
      721 tattcttgtc gcataatcgt ataagtatcg gctaaagaag ggactttcaa ggcagcgctg
      781 actgaaccag tggcaatcat caaatctgct tctcttgcga cgattgcaag atcttggttg
      841 attttttttg ttttttcgct tccgccagtc atcgcattga tataaaatgg gctgcttagt
      901 gtaaacccaa ctgcctgtgt acttagatcg acatcagaaa ctgctatttg cggcaaaggg
      961 ttatggatga tccgtacgaa gtcaaattcg tttttttgtt tatcatgaaa ggctttagct
     1021 agtgaaacat gttcgtcttt tcgattcatc ggttttcctt tctttgatcg ctatagacat
     1081 gtaacggcag tggagtgatt tctgcttttt cccatgcact cattaaagga agaatgccag
     1141 atttctggtc aacaattacg attccgcaat cgcccccacc tgcaccagaa gattttgcgg
     1201 ctccttcata ctgttcagct aaattacaca atttgttcaa agcaggcgtt tcgatgacga
     1261 ccccagtgat tgcagataaa tcatgcagta attgtcggtt ttttcgaatc atctgttgga
     1321 tcaacgttac attattttct ttaaaccctt tgatcatttc gttgacacat tctgtactat
     1381 tttttaagaa aagctgataa gccaccattt tatcttctct cgaacggtga acttgatcga
     1441 ccaaatcaga agtagaggca gggctacccg tccaaccaat caataaacgt aaatcttcag
     1501 gagcaatcaa tggctcaatg gatagacctg gccaatccag ggcaagtaac tcactgatag
     1561 aatgctgagt ttcttgttct tggagccaag gatggtcgaa ggttgagaaa gcgatccagc
     1621 caccataaca gctagcagcg atgtcgccgc aggaaccatt atcttgaact gctaaattgg
     1681 ctagtgctgc aatcttgaaa atctccaact gagataaatt caacgcgtaa aaaacattca
     1741 aggctttgac cgttgcaacg gtcacggcac cgctagaacc caatccatat ttccggccat
     1801 ttgaactgtc taactcactt gttactttca gatcataaaa ggataaaaga atgttttttt
     1861 cttgtgcata cttttcagtc aagcgaatag cagcaaggat ataatgaaaa ggattttccc
     1921 gaatatctaa aactaattct ccgttgcgtc ttgtccaacg tacaggcatc ccactatatt
     1981 gtgcagattg gatacttccg acttttcgtg cagattctac agtgactgtc acgaattgat
     2041 cgactgcagc gataactgct ggatggcctg tttcaacaac tgcatattct ccggcaatat
     2101 agagtttgcc tggtgcagat acttcaatca taaaaatctc cctttattca ataatcgcaa
     2161 ttcctggacc agcatgtgca gtaattactt gttctttgct aaataatttg gtaaatgttt
     2221 cttgtacctc agataaatgg ctgttttcca ctaaaacttt gacattaggg ccagcatcca
     2281 tcgtaaagta gcatggaatg ccttgctttc tcaattgtcg caccgcatcc atcgctttta
     2341 agctgtcagg agaccagtaa gtgaatggag gctgggcagc taaagtcgtt ccatgcattt
     2401 ttagtccgtt tctttccatc gtttctccga gaagttggaa atcttttgtt ttgattgctt
     2461 gtttcaattg atatagatcc ccttcaacgg aatctaacca gccttgataa aaactggatg
     2521 tttcgactgt ccgacgcatc ccatttctgc tggacacttc ttttttctgg tcgttgatca
     2581 aaacgaaaac cattgctaaa tcgtcttcga aagaatcgga agggactggc ttagcgtaag
     2641 aacttaagtc gtcatggcct ttttcccatt cgacgaaacc accgaaaata cttcggcaag
     2701 ctgaaccaga cccgcgtcga gcaaatcttg aaagagagag atcatctaat ccaagcttaa
     2761 gagcagtatt gcaagcgcct gctaaagcag ctagcccgct ggcagaggag gctaatccag
     2821 ctgcggtagg aacgaaattc tgactgatta ctgaagcttt tagtgagagg ccggcttctt
     2881 tacgaaaaag gtcaagaaac tgactgactt tttttgttgc tttttcgtct tgcaaggtgc
     2941 catctagata aaattcatct accatatagc tatcagaaaa gatgacttct gtctctgtat
     3001 aaaatgcatc caatgttaac gaaaggctgt tgttcattgg aaggatgagt tcttcatttt
     3061 tcttacccca atatttgatt agagcaatgt tggtatatgc gcgtgctttg cctttaaaca
     3121 taggtatgta ctcctaatcc ttgtatccaa gtttcagcag cacccgcatc ttcaagtgct
     3181 tgactgattt cttgggcttt tgtttttgtt tgtgctaatg caatcataca gccaccgcgt
     3241 ccaccgccag ttaatttggc acccaatgcc ccggtatctt ttgctgtttg gatcaataga
     3301 ttaagaaaat cattgctgat tgtcagcttt tccagaagtg attgtgattc atccattgtc
     3361 tgtgccaacg tttctggtga attttcaatg attgcttgtt ttgcttgctt cgtcaagtat
     3421 cctaattttt ggatcatttg tccagtttca tgaggctggg tttcaaagag gtgagcgaca
     3481 tctttgacgg cttctcttgt ttgtcctttg attcctgtat cagcgacaat caaaaaagca
     3541 tcgatgttca aagaaaagta gtcgaaagga tgcccttttg taaaataaat cggctgcaag
     3601 ctgctagtag cggctgcatc gattccacta ggattaccgt gggcgatttt ttccgaaagc
     3661 tggacatttt ctaatagtat ttcacgagac aaaggaaatg ctaagtaatc ataaaaagca
     3721 cgagtgactg ctgtggcgac tgcagcgctt gatcccattc cacgttcagc aggaatcgtg
     3781 ctttcaatgg tcagttgcaa gtttttttgg atgtcatgct gtttttttaa gtgaatgaat
     3841 aattttttga tattttttaa tgcatgagga gcttcggcta aatttcctga atagtaggaa
     3901 gaaaccagtt gatcgtccat tgcatccagc tcttcaagga atgcggtgac ttttgttgca
     3961 tagaaaggaa aggcaatcgc tggttctcca taaacaaccg cgtgctcgcc catcaatatg
     4021 atctttccgc ttgattctcc ttggccatag tttgccataa tctaactcct tctcacgtaa
     4081 aagtgatttt taagcaaaaa ataaagcgcc caaaacgagt tgcgctgctt cacgaatttt
     4141 tttgtccact aagggtctca aaaaacattc ctcctttatt ttactaaaac catctcagaa
     4201 gtaaaactaa gaatacgttt tctaaagaaa aaacactttt ttcttaagaa ttttttgact
     4261 ttttttcttc tttgactaga aaaaaataca attgacacta ctagatattg ggggtatatt
     4321 taaaaaggtt ggagttaaac acaatattta gtgatgaagt gagctggagg taaatgagcg
     4381 tgatcgtttt agcaggtaca attggtgccg gaaaatcaag tttaacagaa atgatggctg
     4441 aacactttga tagtcaggca ttttatgaat cgattgatga taatgaagtc ttgcccttat
     4501 tttatgcaaa ccctgaacag tatgcttttt tgctgcaaat ttattttttg aataaacgtt
     4561 ttgcaagcat caaacaagca atgaaagatg acaataatgt attggaccgt tcgatttatg
     4621 aagattcact gcttttccat ctaaatgcag atctaggaag agcaacagaa acagaagttc
     4681 gtgtgtatga tgaactgctg gaaaacatga tggaagaact tccctatgct gcccataaaa
     4741 aacatccgga tttattagtt catattcgtg tatcttttga tacgatgttg gagcgtatcg
     4801 aaaaacgtgg tcgttcgtat gaacaattgt cctttgatcc aagtctatac gattattaca
     4861 aagaattgaa cagacgttac gatcaatggt atgaagaata caaagaaagt ccaaaaatcc
     4921 aaatcgatgg agaccgctat aattttgtgg aagatcctca agcaaaagaa gaagtcttga
     4981 aaatgatcga agaaaaatta gcagagattc gccaaaccaa agtagccatt tgatttacag
     5041 ttgctcaaat aaagggtaaa cctttatttg agcgactttt ttctttatac tggaaacaaa
     5101 tgtcaggagg atagagtatg cgggaaaaac actattttaa tcaagaccgt aatttttgga
     5161 tcaatgtaca gcaaaacgat gtgctagctg ttaaaaactt aaaagaagaa tatggcatta
     5221 gtgacgagat gctgacttat tctctagata aaaatgaacg ggcgcgcgta gagtacgatt
     5281 cttttgatga agcgttactg cttgtatcaa atgttccgca ccagcaaaaa gtcgataatc
     5341 attatgaaac tagtcctatc gcttttattt taaaagaaga cggtttgttt acatttacca
     5401 cgccgaatac cgagtacgtg attcgactaa ttcgatcgct tttggaacga atgccagaca
     5461 tgagtgtata cagtttattg tttcgtacat tgtttttgat ttctgattcc ttttttcctt
     5521 taatcgagga agtgaatagt gaacgtcagc ggcttaatct gaaattaaga gaaaaaacaa
     5581 cgaacaagaa tctgctgcaa ctttcagatt tagagatcgg tttggtttac ttagttacag
     5641 gaacaaaaca aaatgtagta cttttagaac aaatcaaggc attggctatt tatcgcaagt
     5701 tgtcagaaaa ggaaaaagaa gagttggacg atgcgctgat cgaagcacga caagcagttg
     5761 aaatgacgaa cttagcttcg cagattttag accagctctc tggcacttat aataatttgc
     5821 tgaataacaa cttgaatgac acgatgaaat ttttgacggt ctggtcgctg atcttgaccg
     5881 ttccaactat tgtgacagga ttcttcggaa tgaatctcca gttacccttt acccattccg
     5941 ttttcggatg gggaatcgcc ttgatcatca gtttggtctt atctatttgg atgcttattg
     6001 cactgtggcg gcggattcgt tgatctagtc tttgatctgc aaaccaaggg cttgggcgat
     6061 cacaagtgct tgctcttggt tgacgattag tcccgccaaa gaatcttgag ataaagcgat
     6121 tgctgtgaag aaattagtcg taaagtctaa tcccttcaga gaagttcgaa accagttcga
     6181 accggtcaac tgattgtcag acaaaaaaag gtttttccaa gtgacctcat aaaattcgct
     6241 gtcttctaat tgacaatgat cgaaggagac agctttcaag tttgtactac taaaggaagc
     6301 catcgaagca atacaatcag tgaaagtaca gtctcgaaga taactttcgg caaaattcgt
     6361 acctgttaat ttacattgat gaaacacgac ttggtggaaa ctgccaccaa tccactccag
     6421 attggaaaaa tcacattttt caaaaatgac attactgcat tcaaaatgac gtaaaactgt
     6481 tttttgcatc gtaagttttt ggatatgact ctctcttaat accatatttt tggcatcgag
     6541 atagctttgg tcacccccgt tgatcagaag accagagaag atctcctcat cttctaaata
     6601 aaactctccc tttgtaagaa gaggaagaga aggtggaata ggttcgattt ttttcatgtg
     6661 ggttcacctc gtttttcttt agtatagcaa agaagcggga atcactgcta gaaagtctgc
     6721 taatctaatc attttcagaa aaggaagaca aactgtatga aaaacattga caaaaagatt
     6781 agtttttgct atactatgat ggttgagaaa taaaagcaga agcacccgct tctcgcctta
     6841 gttgataatg tcactgggct agatagtttt cactggttct attaggtgaa gtacgcgcgg
     6901 gttctgtaag agaactcgtt tttttattgc cgtttattcg gtaagctttt ctttctctca
     6961 taatatttgg aggtgaatga ccatagcaaa ggatatgatg gttaacgacg gcattcgtgc
     7021 acgagagtta cgattgatcg gttcagatgg tgagcaatta ggagttaaga caaaagcaga
     7081 agcattgaat attgcagaac aagcaaactt ggatcttgtg ttagttgccc ctggtgcgaa
     7141 accaccagtt gcgcgaatca tggactacgg taaataccgt ttcgaacaac aaaagaaaga
     7201 ccgcgaagcg cgtaaaaaac aaaaagtgat caatgtaaaa gaagttcgct taagtcctac
     7261 gatcgacttg aatgacttca atacaaaact tcgtaatgca cgtaagttct tggaaaaggg
     7321 cgataaagtg aaagcttcta tccgtttcaa aggccgtgcc attacccata aagaaattgg
     7381 tcaaaaagtc ttgaatcgct tagctgaaga aactgcggat attgcaacag tggaacaaaa
     7441 agcgaaaatg gacggacgca gcatgttctt gacgctggca ccgaaaaacg acagtaagta
     7501 atttgaatga ctaactggct gttggacagc cgatagtcgg ataacgaaag attttgagga
     7561 ggaaatatag tcatgccaaa acaaaaaaca caccgcggat cagcaaaacg tttcaaacgt
     7621 actggtaaag gcggattgaa acgcttccgc gcgtttacaa gtcaccgttt ccacggcaaa
     7681 acaaaaaaac aacgccgtca attgcgtaaa gcaagcatgg tttctagtgg cgacttcaaa
     7741 cgtattcgcc aacaactagc aaaaatgaaa taagaagtac aaataacaga atctgaagat
     7801 ttatttttat taggaggaat tatacatggc acgtgttaaa ggtggaacag taactcgtaa
     7861 acgtcgtaaa agagtgctaa aattagcaaa aggttactac ggttcaaaac acactttatt
     7921 taaaacagca aaagaacaag taatgaattc atacaactac gcatatcgcg atcgtcgtca
     7981 aaagaaacgc gacttccgta aattgtggat tgctcgtatc aacgcagcag ctcgtatgaa
     8041 tggcttgagc tactcaaaat taatgcacgg tttgaaagtt gctgaaatcg acatcaaccg
     8101 taaaatgtta gctgacttgg ctgttaacga tgcagcagca tttactgcat tggctgacca
     8161 agcaaaagaa gctttggcta aataagctaa acaattataa agaggaaaga agataacgaa
     8221 aagttatgtt ctttcctctt tttttattta caatcctcca tcaaatgaaa caggacaata
     8281 acgtataagt ctaaaatagc ataattaagg ttttttaatt gaaaactgaa catttatggt
     8341 caccctttaa tgaaagcgct aactacataa gttagaatga gtttgactga tatattagtt
     8401 tgcttatcag aaaaacaaaa tggacggagg aataacatga gtgatattat tattggtatt
     8461 ttgttagtta tctcattctt tttcatggtc tggtactgtg taaaaggcta caacttgatg
     8521 gtcggctttg ccatcatggc tactgtatgg atgggattag cgctagtcgg aaacacgttt
     8581 tcaccaaacc cggcgatgga agggcaagga gtaattgatg tgttgacgca tatctatacg
     8641 acggggccag ctgagtacgc caaatcgatc ttggtaaatg tcttctttgg tgcctttttc
     8701 gggcgtgttc tagtagatag cggaatcgca gctaccctga ttcgaaaggt agttgaactt
     8761 ggaggggaca agccaagaat cacgatgagc ttactatgta ttgtaaccgc tgttatattc
     8821 atgtcgatga caggaatcgg accagtgatt tctattgcag ttattgtgtt gccaatcttg
     8881 atgtcattag gtatttcagt accggttgca ctgttctcat ttatgggctc gattatggct
     8941 ggaatctttg ctaatatcgt caatttcaaa caatatcaaa cgatttatgc tggtttcaat
     9001 ccagcggctg aaagctatac ttataatgac tatttccaaa ttggtatgat tgggatgatt
     9061 gtaagcttag ttgtggtact aacggtcgct aatatatcaa tgaacaaaaa gaaacgctat
     9121 gcaatggcag caaacgtacc agctgaaggt ggcgacgcac caatgatctc atggttagcc
     9181 gtattattac cagttttagg ggtcgttctt ttagatcttc caatcatctt aggttttatt
     9241 ctagcaggta tctgggcatt gctgtttact ggaaaattgc gcggaggtta taaagaaatc
     9301 tgtcgtcagt ttgcgaaatt atttacagat ggcgcagtag atgttgcacc aatggttggt
     9361 ttcttgatga ccttagcgat gttcaataat tcagctgcgt atgcttctcc ttatttttca
     9421 gctatttttg gagattggat accgcaatca ccattagtct tggcaatcgt atttgctata
     9481 ttgacaccac taggattttt ccgcggtcca atgaatctag tcggttcagg ctcagctatt
     9541 ttagctgtcg tattagctgt taatccaacg atgtcgccag cctttttgtt cccgttattt
     9601 gcaatcacaa cgattgctcc tcagcattta gatattacac aatcttgggt tgcttgggga
     9661 ttaggttata caaaagtaac ttctcgagaa tatatgaaga aatccatccc aactggttgg
     9721 atcatcggag cgatcttatg cctgatcacg ttcttattgt acgggaacgc ctagtaaaca
     9781 aaacgtcgat aaaaacgatt agatgaatgg aggaaaagaa aatgaaacgt tttgtgcgaa
     9841 tgggaattga tgttggcggt acacatacga aagcagtcgc aatcgataat gcgacgcacg
     9901 aaatcatcgg aaaatcttca gtgaaaacaa cccacgatga tgtccgtgga gtagccgcag
     9961 gtgtcgttca gtcttttcaa aattgtttgc gggaaaataa tatcagtcca gaagatgttg
    10021 tgtttgttgc acattctacc acacaagcga ctaatgcact gattgaagga gacgtagcaa
    10081 aagtcggcgt aatcgggatg gcaaaaggtg gattagaagg ttttttagca aaacgacaaa
    10141 ctagattgaa cgatatcgat ttagggaata agaaaaaaat agagattgtc aatgcattct
    10201 tacctgtcaa acacttaaat gtagaccgtg tttcagaaac gatttcttct ttagaaagag
    10261 aaagagcgga ggtcttggtt tcttctatgg catttggagt agataatggg gaaccagaac
    10321 gagttgttta tgaagcagca tcggtaaaat cgatccctac aacgatggct tctgacatca
    10381 caaaattata tggattgaca agaagaactc ggacagcagc aatcaatgca tctattttac
    10441 caaaaatgct agatacggca acctctacag aagattccgt acgagaagca ggggtcaatg
    10501 tttctttgat gattatgcgc ggagacggcg gagtaatgga aatcaatgaa atgaaaaaac
    10561 gacctgtttt aaccatgctt tccggtccag cagcatcagt catgggctct ttgatgtact
    10621 tgagagcgtc taatggtgtt tattttgaag taggcggaac aacaaccaat atcggggtca
    10681 ttaaaaatgg acgtccagca atcgattatt caattgtagg tggccaccca acatatatct
    10741 cttcattaga tgttcgggtt ctaggtgtgg caggcggttc gatggtacgg gctaatcagt
    10801 caggaatcat cgatgtcggt ccacgttctg cacatattgc cggtctagat tatgcagtat
    10861 ttacggagac agaaaaaatc aaaggtccta aggttgaatt tttctctcct aaagaaggag
    10921 accctgctga ttacgtcaag gtagtgatgg aagacggaga agaagtgact attacaaata
    10981 catgtgcagc caatgtcctt ggattagtgc aagaagaaca tttttcttat ggaaatgtcc
    11041 catctgcacg aaaagccatc caagcattag ctgattattg tcatacgacg gttgaagaca
    11101 tcgcagaaca gatcatggaa aaatcctatg ctaagattga accggtgatt ttagaattgg
    11161 cagacaagta tcacttggag aaagatcaga tttctcttgt aggtgtaggg ggcggcgctg
    11221 cctcattgat tacttacttt agtaataaaa tgggtgtgaa atattcgatt cctgaaaacg
    11281 ccgaagtgat ttcttctatt ggtgtagcac ttgcaatggt ccgagatgtt gtagaacgta
    11341 tcatcccttc gccaagtaaa gaagatatac gatcgttgaa aaatgaagcg atgaataaag
    11401 cgattgaatc aggcgctaca ccagaatcga tcgaagtcca cgtagaaatc gatccacaga
    11461 cttcaaaagt aacggctatc gctactggtt ctacggaagt gaaagcaacc gatctaacca
    11521 aggagatcac aacagaagaa gcactagagc ttgctgcaga ggatatgcga ttaaataaaa
    11581 atgaagtttg cttattggaa aatacacctt tcttttatgt atgcggcgaa cagaatcgtt
    11641 caaaaaatgc tggaagtctt cggatcatcg atcaaaaagg atttatcaag gtccaacgtg
    11701 gacatgcttc atgtatgaaa acaactgcag ccaattatat gacagctgtc gaacaacttt
    11761 gggaagatat ggcagtttat caaactgaat tgattgcccg accagaattt tatctctgct
    11821 taggagcaag agtctctgat tttacagcga cggatctgga acaactacag cttttgatgg
    11881 atctggaagt atctactatg gaaccggagg aagaagtgat cgtcgttgcc ggaaatatca
    11941 aacaaacata agttagaaga gttactaaac gaattacaac agatccccga agaaatttgg
    12001 ggattctacc agtttcggcg agatttattt tggaaaaaga ttcctttgtc caagcagaaa
    12061 attcttatac aacaatcaat cgattgtggt atcgaaacgg catgtagcat caaaaaaaag
    12121 tacccttttg ccgatgtagg agaaatttgt gaacaaatgg ctgttccaat cgtttcctgt
    12181 gaatcagagc aaataaacga acgaatcact tttgcaactt atgcagaaga tgaagggatt
    12241 cgattgatga cggagccgtt agaaaaatta aaatgttcag gactagcttc gatctctaaa
    12301 gaaacagcgc aagctttgat tattggacat gaactatttc accacatcga agcttctgtt
    12361 aagggaattt atacgcaaag cgaaaaaatt gtgctgtggc ggttgccttt ttatacacat
    12421 caatcaaaca ttcgtgcatt gagcgagata gcagcgatga gtttttcaaa agagatgaat
    12481 caaagcgatt tttcgcctta tgtattagag gcagtcttac tttggccata taatgaaatt
    12541 cgtagccaag ggatcttaga agaaataaaa aaaatagaaa aaagatgtac ggagtatgat
    12601 ttagattaca aataaagaga ctgtgacaaa agtcgtgtca cagtcctttt tttccgaata
    12661 aacgatgttc tcctgtcaac tcctcggtca caagtca
//
LOCUS       DBNICI010000080        12630 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.080, whole genome shotgun sequence.
ACCESSION   DBNICI010000080 DBNICI010000000
VERSION     DBNICI010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.080"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(194..991)
                     /locus_tag="AC1ZCC_001756"
     CDS             complement(194..991)
                     /locus_tag="AC1ZCC_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HIQ1630004.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(988..2172)
                     /locus_tag="AC1ZCC_001757"
     CDS             complement(988..2172)
                     /locus_tag="AC1ZCC_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HIQ1630005.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2197..3444)
                     /locus_tag="AC1ZCC_001758"
     CDS             complement(2197..3444)
                     /locus_tag="AC1ZCC_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIQ1630006.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3659..5404
                     /gene="dnaX"
                     /locus_tag="AC1ZCC_001759"
     CDS             3659..5404
                     /gene="dnaX"
                     /locus_tag="AC1ZCC_001759"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIQ1630007.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5424..5738
                     /locus_tag="AC1ZCC_001760"
     CDS             5424..5738
                     /locus_tag="AC1ZCC_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIQ1630008.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5763..6359
                     /gene="recR"
                     /locus_tag="AC1ZCC_001761"
     CDS             5763..6359
                     /gene="recR"
                     /locus_tag="AC1ZCC_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIQ1630009.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6423..7067
                     /gene="tmk"
                     /locus_tag="AC1ZCC_001762"
     CDS             6423..7067
                     /gene="tmk"
                     /locus_tag="AC1ZCC_001762"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIQ1630010.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7082..7411
                     /locus_tag="AC1ZCC_001763"
     CDS             7082..7411
                     /locus_tag="AC1ZCC_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HIQ1630011.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7425..8363
                     /gene="holB"
                     /locus_tag="AC1ZCC_001764"
     CDS             7425..8363
                     /gene="holB"
                     /locus_tag="AC1ZCC_001764"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HIQ1630012.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8399..9223
                     /locus_tag="AC1ZCC_001765"
     CDS             8399..9223
                     /locus_tag="AC1ZCC_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSP1 domain-containing protein"
                     /protein_id="HIQ1630013.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9216..9563
                     /locus_tag="AC1ZCC_001766"
     CDS             9216..9563
                     /locus_tag="AC1ZCC_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HIQ1630014.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9632..10504
                     /gene="rsmI"
                     /locus_tag="AC1ZCC_001767"
     CDS             9632..10504
                     /gene="rsmI"
                     /locus_tag="AC1ZCC_001767"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIQ1630015.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10613..11734
                     /gene="dinB"
                     /locus_tag="AC1ZCC_001768"
     CDS             10613..11734
                     /gene="dinB"
                     /locus_tag="AC1ZCC_001768"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HIQ1630016.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11788..12390)
                     /gene="nrdG"
                     /locus_tag="AC1ZCC_001769"
     CDS             complement(11788..12390)
                     /gene="nrdG"
                     /locus_tag="AC1ZCC_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HIQ1630017.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
BASE COUNT         4016 a         2369 c         2708 g         3537 t
ORIGIN      
        1 caagaggttg tgaaaaagct gttctgcatc aagtaataag aagagccaag taaaaacagt
       61 ctcttatgtt tttaacttgg ctggacttat caccgtagat gtaagctttt gaacgccgtt
      121 tatttgcaac aaaaggggct gtgacacgat ttttgtcaca gccctttttt tgcgaacatt
      181 catttagcaa tttttagaaa cttctgccgc caccgccaaa agtcccgcca ccgctagaat
      241 gggtagtact tccgccaccg ccgcttccag gaggtgggct tttggggatt cttcttgtcg
      301 tgacaaaaga atttgtcaga cgatcggttt tttcagttaa tttgatactg gatttttctc
      361 ggaatggata tttgtagatg cctgatttca attgataacg cgaaacagtc acaaaataaa
      421 agacaagact aaggataaga gccaaaacaa cagcaatgat gatttctact gtcgtcaaaa
      481 ccttatagcg tgtaattttg ccagtttctt cgtctacacg ataatgtcct cctggaactc
      541 cgtcttccac atatgttgct gatttagaca ggtaagcttt tgcagcattg taataggcag
      601 catttgtcat ctgatcataa acatcatcta agatcgaatc gattctgcta tcagtcaaat
      661 aatcgatcat attaccagaa gtcgaaatgt agatttcacg ctgtcccata tctaatagca
      721 atactgagcc attttgatca tttccgactg cgtctcttaa gtaattatca gcaaagtctc
      781 ttggttcttc tgaattagat gtagtcgtta cgatcaagac tcgtcctttg attttttcat
      841 tcagcttttc tgcctgttgt tccaaagatt ggatttgatc tgttgtgaat aacccagcct
      901 catcatttac cgtagctgta tctgcatgag tagccaccgg ccagccaaca cttactagca
      961 aaagaaagaa taaaaaaaat tttttcatca tatcaagtaa cctccgatca gaaagaggat
     1021 cgctagccca gcgaaaatcc caagagcggt cagtcccaat tttttctggc tgatcggcag
     1081 gacaccgctg acctttcctg tttgtccatt cattgcgtag tagtagactt ttttacttga
     1141 gtcattgctt cgataagtaa caagccatac cggcagtaat acgtaattat ttacttcgtt
     1201 tgtgattgaa gcagaagttc gcacaccggt caatgttgta tatccatttg cggtatcttt
     1261 taacagcttc tctgaatagt cttttaattc agattgaatc tgtgatttga ttgcctcata
     1321 ttcgatatct cttttttctg cttggaaccc ggctaaatac tggcttttaa aatcaattgc
     1381 tttatctaac gggaaaggtt gcacgccttc gaccatcttt tgttgcgtat tttttgataa
     1441 ggcatttttt actagttctt taaaagaaag cttgcctgct cttgaaacag caaattgctt
     1501 cgtttctgtg tattcgatgt cacctatccg ccatatgcgg ataactgtac cattcgcctg
     1561 catggcacca tctacttcag catctgtagc ccaataaggg aaatacacac ctgtcaactt
     1621 atcgatttga tccttattga aaaaagcttt gggaacaaac cattttttct tggtccaagc
     1681 gagaaatctg ttgattgctt cttctttctc gacagcaaaa ggaagtactt tatttggcaa
     1741 gaattctccg cttaaacgtc ccgatagtac gacgggatta tggcagtagt aacaataagt
     1801 cgcagcagtt gtcgcatcag tcacgatctc tgcaccacag ttgggacaaa gaaaaagatc
     1861 tatcgctgcg gtattatctg cagattcgtc cgctgtatct gaaaaaccgc ctgcctcgtc
     1921 aaccgctttg cgctcggctt cattcatatc ggcaagctgt tcttgggctg taaaagtaaa
     1981 atcttcggta ggtgaaggtt cttctttgct ttcttcgcca gccatacgcg cttctttttg
     2041 cttttgctca tattggctga cttcttcttc tgtatagata ttcaggcagt attcgcaatg
     2101 aaatttttgg tctttgggat caaaagtcaa cggaccgccg caattaggac atttatgtgt
     2161 aagaacatcc atcagctgca cacctttcta aataaattaa ttaacgggga ttcctttaaa
     2221 cggtttacca cagtttggac agaatttagg gatgccgttg gaaagatcca cgatctcatt
     2281 gcattcactg catcgcattt gtagcttagg ctgaacgttt tctacaggtt tggcagctcc
     2341 gcagttggaa cagaattttc cggaattttc tgtcccgcaa actggacatg tccaagtatc
     2401 cgcatttcct tgtgcagttt gttgattttg tttttctgct tgttgtttga tttgttcttg
     2461 gttattttga gctgcttgag aaagataaga accatttgca ttcattccca tacctacacc
     2521 cataaagccg gtcattgctc ctgcttcatt ttttccggct gcttccatac cacgtgcaat
     2581 ggaaccttgc acgtatcctt cacgtacgct aggatcacct agcatcgccc cttcgttacg
     2641 catattgatc agtttcacag aatcatcggt gtaagaaatg ctggctacag ccacagaaac
     2701 aatctccatc ccacgtaatt cacgccaaga atcatccaat gcagtatcca tgtatttgct
     2761 taattccaaa ctcttagatg gtacataaga aatccgttga ccatctgcag acatctgatt
     2821 gattgctgat tgtagcgctg ttaggaattc agctaaatac tgctcattga tatcattgat
     2881 ttccacttgt gttttgtttt tagggattgc gtttgtataa aataaaatcg ggtcaacgac
     2941 atgaatagaa taagtgccat gtgcccgtaa gaataattct gcgttataga aattgtcaaa
     3001 gtagttcaac ggcgcgcttg taccgaattt gattcccttt atttcttgga gattgatata
     3061 aaaaacttgt tgtttctgcg gtgtgacgcc gccaaatttg aatcggtcaa aagtttcagc
     3121 aattgcttct ttcagtgttc cattgaacat ggaaggagct gcgtcatttt taatggtgta
     3181 ataaccttcc tcagcggtat aatcgatgat ttttccacca tcgaccaaaa gcatcatcat
     3241 gtttggatag acatggacga ctgttccgtc tgttaaaaca tcttctgttc ctttacgatt
     3301 ggagccacgt ttgtcatctt tgcggacttt gacacctttt gtcatgaccg ttgtatcact
     3361 catattgtct ggttcgatga cttctagcca ttgatctgcc agaccgccgc caaccatact
     3421 tgttgctgct ttgataagac ccataaaaaa ttcctcctct gattcagcta gtgccaaaca
     3481 attattgttt atttattata ccataggaag atgtgaaact tctcggacta gagacggaat
     3541 tgaaatggaa aattgcgtat tgtttaaata cctgaaaaag ccgagaagga tagtagtttt
     3601 tttacctaat atcacgtata ataagagata gtgaattttt taaaaaaggg ggatccttat
     3661 ggcatatcaa gcactttatc gagtctggcg ttcgcagcgt ttcgaagaca tcgttggaca
     3721 aaaagctgtg acacagacac taaaaaatgc tattgtttcc cacaaaacct cacatgccta
     3781 cttgtttaca ggacccagag gaaccggaaa gacgagtgca gcaaagattt tcgcaaaagc
     3841 aatcaactgt ccgaacagta aagacggtga gccatgcaac gaatgcgaaa tgtgtcgatc
     3901 aattactgct ggtacgcaag aagatgtgat cgaaatcgat gctgccagca ataacggagt
     3961 cgaagaaatc cgatttatcc gtgatcgggc aaattatgcg cctacaaagg cagcgtacaa
     4021 agtgtacatt attgatgagg tacatatgct ttcaacagga gctttcaatg ccttgttgaa
     4081 aacattggaa gagccaaaag aaagtgtcat attcatctta gcaacgaccg aacctcacaa
     4141 aataccagct acgatcattt cacggactca gcgatttgat tttaaacgga tcaatacggc
     4201 agatattgtc gaacatcttg aatttatctt gaaagaatcg actatcagct atgaaccagc
     4261 tgcattgaat gtgattgccc gttctgcaga aggcgggatg cgggatgcat tgagtattct
     4321 tgatcaggca atctcgttta gtgaaggaac aattacttta gatgatgcca tgcaagtgac
     4381 aggtagctta actgacgaaa tgatggacag ttacttgagt gcttgcgtga aaaaagaagt
     4441 accagaagca ttggaaacat tgaaccaaat tctcgcagcc ggcaaagaat cacgtcgctt
     4501 tctagaggat atgttacaat actgccgtga cttgctgatg tatcagcaag cacctaacct
     4561 agttgaggaa caatcgagta cgatcagaag tacatttaca gaattggcac aaactgtttc
     4621 agcggatcag ctgttttcaa tgataaagat tttgagcgat actcaaaatg aaattcgttt
     4681 taccaatagt gccgctattt atttggaagt cgctacagtc aaacttgcac agctaccgtc
     4741 tgcttctgtt ccttcagcag atgtagcatt tgcagatgat cagatagtaa aacagctaca
     4801 acaagagatt aaggaactaa gaaacgaaat caaggaacta cgcagtaata caggaattga
     4861 gaatacaaaa gtacagccgg tacagcaaac aaatcataaa aaacagacaa gtacctatcg
     4921 tgtaccgacg gaacgagtat ataaagttct gcgggaagcg acaagacagc atctgaacca
     4981 agtaaaagaa gtatgggaag acttgctgat gagtctatct gtcactcagc gagcaatgct
     5041 taaagcaagc gaaccagttg cagctggacc tacaggacta gtgattgcgt ttgattatga
     5101 gattgtttgt caaagagcag caaatgacga agaattgcag ctttcggtac acaacaactt
     5161 aagccgaatg atcaaagatt atgcgcctga tcctgtttat attacaagag aaagctggcc
     5221 agaacttcga agaaattatt tgacgcaggc acaagaagag ggaacagaaa actcttttgc
     5281 ttctgaagac tcagaaggag aaatcgagtt gcttcctcaa gaacaagaag taccagaaga
     5341 aacacttgta gtaaaagcag aggaactgtt tggctccgaa gctgttacag tggtagatga
     5401 ttaataaaaa ggagtagata attatgatgc gtggaatggg caatatgcaa ggcatgatga
     5461 aacaagtaca aaaaatgcaa aaagaaatgg cagaagcaca agagctgcta aataaaacag
     5521 agtttacagg taaagccacc aatgaattgg tcaaagcggt ttttacaggc gatcgtcgca
     5581 tgaaagatat ccaaatcaat gaagaagtcg ttgatcctga tgatatcgag atgcttcagg
     5641 atcttgtcgt aatggcagtc aatgatgcga tcgcacaaat tgatgtggaa actgagaaaa
     5701 aattagggaa atacgcaaaa ggtttgccag gattttagtt ttaaactgaa ggagtaatga
     5761 atatgcaata tccagaacca attgcgaaac tgatcgacag ctacatgaaa cttccaggaa
     5821 tcggtcagaa aacagcgaca agactcgcct tttatacgat cgatatgaaa gatgaagtag
     5881 taaatgaatt tgctaaatct ttgttaagtg taaaacgcga tttgcatttt tgtagtatct
     5941 gcggaaacat cacagaagaa gatccctgcg aaatatgtag aaatccttca cgtgatcgaa
     6001 gcgttatcct agtggtcgaa gagccgaaag atgtaatggc aatggaaaaa atgcgagaat
     6061 atcacggact ttatcatgtt ctccatggtg tgttatcacc gatggaaggg acaggtccag
     6121 aagacatcaa tatttcttct ctgttgcagc ggctgcatga tgcagatgta aaagaagtga
     6181 ttatcgcaac aaatgcaaca acagagggag aagcaacagc tatgtatctc tcacgtctga
     6241 tcaaacccgc aggtatcaaa gtgactcgtc tagcacatgg tctgtctgta ggctctgata
     6301 tcgagtatgc agatgaagtt actttattga aagctgtcga aggaagacgt gagttataaa
     6361 tttctaatta agtgtttttt cagatacaat ataaaagcag cacaagagat gggggcaaca
     6421 atgtgaacgg actatttatt acaatcgagg ggcctgatgg tgcaggaaaa accagcatct
     6481 tgaatgaact ttaccctctt ttgaaagaag tagcaaaaac agaaatcatt caaacaagag
     6541 aaccaggagg cattccgatt gctgaagaga tccgagcagt gatccttgat ccaaaaaatg
     6601 atcggatgga tgaacgtacg gaagcactgc tttatgcagc agcaagaaga caacatctag
     6661 tcgaaaaggt tctgcctgct ctggcaaaag ggaaaatcgt tttatgcgat agatttgtcg
     6721 acagttctct tgcttatcaa ggtgcaggcc gacgcatcgg cgttaccgag attgctcggt
     6781 tgaatgagtt tgctacagaa ggtactacgc cggattttac cctttatctt gacgtagatt
     6841 cagacaccgg attaagaaga atcaagaaaa acagacaaaa tcaaatcgac cgtttagatt
     6901 cggaaggttt ggaattccac cagcgtgtac gtcatgctta cttaaagctg gcagaagaga
     6961 atccggaaag aatccacaaa gttgatgcaa gaaaaagctt tgaagaagtg ctgcaaacaa
     7021 gttatcatac aattattgaa cagtatccgc aattttttga aaactaggaa ggtgaacgtt
     7081 tatgaaaatt attttagcga ttatccaaga caaagacagc aaccgtttat caaatgaatt
     7141 gattgatgca aatatccgtg ccacaaaact atcatcgaca ggcggcttcc ttaaggctgg
     7201 aaacagtacg tttatcgtgg gtattgaaga tgaccgtgta gaagaagcat tggaaatcat
     7261 caaaaaaacg tgtgaatcaa gaaagcaatt tgtatctaca cctgttacac tggatatttc
     7321 gatggatggg ggcgtccctt atccagtaga agtagaagta ggcggtgcca cagtcttcgt
     7381 cttacctgtt gaaggattcc atcaatttta ggaggagctg agctatgaat caagaaataa
     7441 acttggctca aatgcaaccg atcgtgtatc agcagcttca acggagtttt gagcatgggc
     7501 gtatcgctca tgcttatctt tttgaagggg agaaaggaac aggaaaacat gaagtaggta
     7561 tatggcttgc acagcacctg ttctgtaccc aaatgaaaga caatctgcct tgtggaaaat
     7621 gcaacaattg tcagcggatc gaaaagaaag aacatccaga tgttttggtt attgaaccgg
     7681 aaggacaaac catcaaagtc gatcagatca gacgtttgca gacggaattc agtcgcagcg
     7741 gttacgaatc aagaaaaaaa gtttttttga tcaaagaagc tgaaaaaatg aatagcagtg
     7801 cggcaaacag tttgttgaaa ttcttggaag agccccctgg agatttttta gcggtgttag
     7861 aaacagatgc agtaggaaga attctcccta ctattcaatc tcgttgtcaa gttctgcatt
     7921 ttcaagaact ttcgaaagaa gcactgatcc atcgattgca agaagagcag attccattgg
     7981 aaaaagcaaa acttttggct ttcttaacaa acagtttagg aaaagcagtt gaaatctctc
     8041 aagatgaatg gtttaatgat gctaaggact tgatttatca atggttcgtt tacttgcaaa
     8101 agcaagatac tcaagccttt atctatgtcc aaaaaaaatt ggtaaaatca tttaaagaaa
     8161 aaacacaaca gtttacagga ttatctgtct tgatgtttta ttatcaggaa gcacagcgtc
     8221 aagcagtagc aaacggcgaa tatggcaaag tgaaacagat aaaccagata attgaacgga
     8281 tattgatcgc agagcagaaa ctacgcagca atgtcagttt tcaggctgtt gcggaacagt
     8341 tcgttttgca tacgattttt tagcttgccg caaaggatga tgatcaagga ggatggaaat
     8401 ggtagaagta gtaggtgtcc gatatagaga tgcaggtcat atctattatt ttgcaccagg
     8461 agactctgaa tatgtttata gtgacaaggt attagtcgaa tctcagcaat ccttacatat
     8521 tgctactgtt gtccttccta aaaaagagat tgatcccaat gaccttcctg aagctatccg
     8581 tccgatcttg cataaagcgt cacaagctga attagaaaaa gacaaaaaaa acaaaaaaga
     8641 tgcttatgat gcattgaaaa tcgcaaaaga aaaagtgcag ttgcgtgagt tggaaatgaa
     8701 aatggtcaaa gccgaataca catttgatcg aaataaattg attttttctt tcacagcgga
     8761 cgggcgaatt gactttagag aattggtaaa agatctggcc gctgtatttc gtactcgtat
     8821 tgagcttcga caaattggtg tccgcgatga agcaaagata ataggcggaa ttggtccatg
     8881 tgggcgtcca ttatgctgtt cttctttttt aggcgatttc gtgcctgtat caatcaaaat
     8941 ggcaaaagat caaagtttat ctttgaatcc aacaaaaatt tccggtttat gcggacgctt
     9001 gatgtgctgt ctgcaatatg agagcgaaga atacgagaca gcgaaaaaag aactccctga
     9061 ctttggaaaa gtaattgcta cacctgatgg taaagggaaa gtcgtagggt tgaatctgct
     9121 tagccgtatc gttagtattc ggctgactgg acgggaaaat cctgtcgaat atgaatggga
     9181 tgaattgaaa gaatttatcc aagtaggtga gatgaatgga taagagatca ttatacgacg
     9241 gattgaatca attagaatca gagttgagaa atacattaac agaacttgtt gaaatgaagc
     9301 atgctttgca tgagatcgta gaaaaaaata caactttgga gatggaaaat caacgtctgc
     9361 gggaacacct gcgggaattg aaccaagttg cacaagctcc agctgaaaat gtgaagcagg
     9421 aactttctaa gtctcgtatg aatcttgaaa aaatttatga agaaggattc catgtctgct
     9481 acgatttata tggatcaaga agggacaatg acgagccttg tgcgttttgt ctagaagtga
     9541 tctatcgcga aagcggtcga tgaccaataa caagaggaga ctggagcgga gaaatactgc
     9601 tccagtcttt ttattaagga gattgtgaca gatgtataat caaaaaagtt tcaagggaaa
     9661 tccggtatat ggggatttat atcttgtacc gacgccaatt ggaaatttag aagatatgag
     9721 ctacagaagt gttcgtatgc tgcaagaatc cgatcttatc gcaagtgagg atacgcgtaa
     9781 tacacaaaag ttattgaatc attttgaaat ccatacaccc caaaaaagtt tacatgaaca
     9841 taattataaa gaacgtgtcc ctcaattgat tgctttgtta aaagaaggaa aaacgattgc
     9901 tcaagtcagt gacgcaggaa tgccttccat cagcgatcct ggacatgagc tagttttagc
     9961 ctgcataaaa gaagggattt ctgtgatctc tatcccagga cctacagctg ggatgacggc
    10021 tttgattgct tcagggctag tgcctcaacc atttttattt tatggcttcc ttccacgtaa
    10081 gaaaaaagaa cagaaagaaa cattgaaaaa gctaaaagaa cagacagtta cgctgatttt
    10141 ttatgagtca ccttatcgga tcactgctac attgacgaat ttcttagaaa tttttggaaa
    10201 tcgccaagtc gttttgtgtc gggaattgac aaaggtccat gaagaatacc ttagaggaag
    10261 tacggaagag ctactcgctt atatcgaaga acatccagta aagggtgaat gttgtctgct
    10321 tgtagaaggg aatttgttcg aaatgccagt gacggaagtt gatgaagagt tgggaacgtt
    10381 aaaggaacaa gtagaagcaa agatcgcttc aggcgaaaaa ccaaatgctg ctatcaaagc
    10441 agtagcgctg aaaaatgggc tgaaaaaaca agaagtctac cgtcagtacc atgaattaga
    10501 ctgagatagt ataagttgtc tataatgaaa gactaatgag gaaatcagct actaactttt
    10561 caaatctctt gaacgcgaac gaatgttctt gtataatgaa taagaggtga ggatgatgga
    10621 gctgcaattt cctttacata atgatacatc aagaaaaatc attcatattg atatggatgc
    10681 ttttttcgct tcggttgaag aacgagatca tcctgagttt cgcggacatc cattagtaat
    10741 tgccagacac cccagtgaca ctggtgggcg tggagtagtg actacagcaa attatgaagc
    10801 aagaaaattc ggaattcatt cagcaatgag tgcacaaaaa gcgtatgagc tttgtccgca
    10861 agccatcttt aagcctggcg accatcataa atacagcgat atttccaaac aagtacggga
    10921 agtattctat cgttatacag atattgtgga accagttagt atcgatgaag cgtatcttga
    10981 tgtgaccgaa aataaaatca attgcaagtc agccatcaaa atcgcccgaa tgatccaagc
    11041 agatatttgg gagagtgtcc agctaacatg ttctgcggga gtaagttaca ataagttttt
    11101 ggccaaatta gcatcagact atcaaaaacc tagaggaatc acggtagttg cccctcaaga
    11161 cgctgtgcca tttttaaaag ctttgccaat tgaaaaattt catggggtag ggaagaaaac
    11221 tgtacctaga atgcatgagc tagggattta tacaggaaaa gacctatatg aatgcacaga
    11281 aatgatgttg attcgaaatt ttggaaaaat gggctattct ctttatcgta aagtcagagg
    11341 aatccatgat tctccagtaa acgttacaag agagcgaaaa tctgtgggaa aagagcatac
    11401 ttatgggcag ccgttgcaaa cggaagaagc agtgctgact cagttgcgcc aactagctga
    11461 aaaagtagaa gaagcactga gaagagtcca aaaacatggg aaaacagtag ttttgaaggt
    11521 caggtacacg gactattcta ctgtgaccaa aagagtgaca ttgccagaat atatctataa
    11581 aaaagaagca ctcttttatc aagcaagcct tatatgggag gaaatcttgg gagtagaaaa
    11641 aggaatccga ttgttaggga ttactttgac gaatctggac ccgatgactt atgaaaacat
    11701 cgtccttcca ttatgggaaa atcaagaaat ataaaaacag ctttgagagt tgaaaccgga
    11761 aatccgtttt ctctcccaaa gctgttttta tatcaagtct tgctttttga tttgttcata
    11821 ggtttctgtt gcatccgtac atttttccca aatcacaact tgattggatt ctaaagattt
    11881 ttggacgtca atgatccgct ggttgctact tccgcgaaat tgtagtttta ggtcacgttt
    11941 gcttaattca aagcgtccgt caacaaggat atcgatttgc gaaaggagtt ctaatttatc
    12001 ttctgaatct tgtagcaatt catcaaaggt atagcctgtc catgaccaga tatcttttgt
    12061 cgtaccgaac tcttttcgga tacgtcgtac gacttgcaga caaacatctg tattcaaaaa
    12121 aggttcgcca ccaagtaaag ttaatccttg cacgtaatcg ttgcgcaagt cagcgatgat
    12181 ttgttcttct aattcaggtg tgaatggttt gccatatcgg aaattttgtg cagctttgtt
    12241 gaagcagcct tcgcaggcaa ataagcagcc actaacgtaa agactgttgc ggacaccttc
    12301 accatcgaca aaattaaacg gtttgtagtc agcaatatag ttttggctgt agtcttttgc
    12361 taaccattct tttggttttg gatttctcat aaaaaaactt ctttcattta aggttcaaag
    12421 atatagctat tgaagataaa atctactcga ccacataaat aagaacaaac tgacctaaat
    12481 ccattgttca agaaagcttg ttcttatttt gatagagggc gttcaaagat acaaagccat
    12541 cgaaagaggg tggtgaaaaa agacttgtca tagcctctta ttctgaataa acggtgttca
    12601 aaagcttgca tctgcagtaa taagcccaac
//
LOCUS       DBNICI010000081        12621 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.081, whole genome shotgun sequence.
ACCESSION   DBNICI010000081 DBNICI010000000
VERSION     DBNICI010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.081"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            4..2376
                     /locus_tag="AC1ZCC_001770"
     CDS             4..2376
                     /locus_tag="AC1ZCC_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HIQ1630018.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIREEIERFEKGESSFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGEFEESKKYQATSEEEKVAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="AC1ZCC_001771"
     CDS             2437..3897
                     /locus_tag="AC1ZCC_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIQ1630019.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="AC1ZCC_001772"
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="AC1ZCC_001772"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HIQ1630020.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6128..6277
                     /locus_tag="AC1ZCC_001773"
     CDS             6128..6277
                     /locus_tag="AC1ZCC_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630021.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6352..6945
                     /locus_tag="AC1ZCC_001774"
     CDS             6352..6945
                     /locus_tag="AC1ZCC_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HIQ1630022.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6958..7857
                     /locus_tag="AC1ZCC_001775"
     CDS             6958..7857
                     /locus_tag="AC1ZCC_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HIQ1630023.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7860..8357
                     /gene="ssb"
                     /locus_tag="AC1ZCC_001776"
     CDS             7860..8357
                     /gene="ssb"
                     /locus_tag="AC1ZCC_001776"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIQ1630024.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8723..8983
                     /locus_tag="AC1ZCC_001777"
     CDS             8723..8983
                     /locus_tag="AC1ZCC_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630025.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9428..9634
                     /locus_tag="AC1ZCC_001778"
     CDS             9428..9634
                     /locus_tag="AC1ZCC_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HIQ1630026.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9682..9885
                     /locus_tag="AC1ZCC_001779"
     CDS             9682..9885
                     /locus_tag="AC1ZCC_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630027.1"
                     /translation="MVDKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFEDLVKIAG
                     YELHYQENMETYENEYWLTKLEN"
     gene            9901..10338
                     /locus_tag="AC1ZCC_001780"
     CDS             9901..10338
                     /locus_tag="AC1ZCC_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630028.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10331..11038
                     /locus_tag="AC1ZCC_001781"
     CDS             10331..11038
                     /locus_tag="AC1ZCC_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630029.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11202..11402
                     /locus_tag="AC1ZCC_001782"
     CDS             11202..11402
                     /locus_tag="AC1ZCC_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630030.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            11418..11597
                     /locus_tag="AC1ZCC_001783"
     CDS             11418..11597
                     /locus_tag="AC1ZCC_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630031.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11922..12185
                     /locus_tag="AC1ZCC_001784"
     CDS             11922..12185
                     /locus_tag="AC1ZCC_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIQ1630032.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12179..12529
                     /locus_tag="AC1ZCC_001785"
     CDS             12179..12529
                     /locus_tag="AC1ZCC_001785"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIQ1630033.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12553..>12621)
                     /locus_tag="AC1ZCC_001786"
     CDS             complement(12553..>12621)
                     /locus_tag="AC1ZCC_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIQ1630034.1"
                     /translation="KDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT         4732 a         2060 c         2608 g         3221 t
ORIGIN      
        1 gacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaatgaa caaatcgaaa agatgtttga acaccaaaaa
      121 atgacgaacg gtcaaccaat gaacgacaag aggaatggcc gttcatggtt taaccagcaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gttataacaa gtacggcgga
      301 ctagatatga cgatcgaaaa tattccaaga atcagagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcgt ctttttcagc cgccacgatc cgaaaatatc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt aacgcaatgg aaaaaaaatc cgaccattta ttttctgaaa
      541 ggctatcgaa aagtcgcttt agaactgaat gaaaacgggg aatttgagga atctaaaaaa
      601 taccaagcca catcggaaga ggaaaaagta gctgttcgtg aattacttga agaggctgcg
      661 ccaactgatt ctctgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggagaga tcgtaacatt agaattgatc
      781 gatcaactca aaacgttgga caaccaatac gctgcagaag caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaagggt tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctatacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tttagaaacg ccagagaata agaatattgt gcccgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagatta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgaaacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaata tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaaag agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagataaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagtcgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aaatcattta tgaagaagtc gcaaaagaag aaaaggtcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct
     3541 cacaaagtaa atgcgttttt tggccagcag ctttctgtta acgattcaat agaagccatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagtttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc gcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg agatccagca acaagaaatc
     3841 agaaagcaaa aacgtgccat gttgtttcaa caaggcagac aacaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca caagcgaaag cttatgccga
     3961 tagcttctct aaagccactc gaaaagacgg gtattttgaa atccaagatc gattgtttcc
     4021 tggtgaaact gtgatcacgt atggttttgg tcatttagtc gaattagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agaacatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatcgcttg
     4261 gacgatcatt caacaagtag gcgccgatca aggaaaaacc tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagacg caaaagagac
     4381 ctatccaaaa tttgaagaag cacaagcacg tcagatcgcc gattggttaa ttgggatgaa
     4441 cggtagccca ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gcttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttgcaggaaa agattagaaa
     4561 tttcaagaaa gaaccttatt ttgaaggaaa atccgaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggcatg attcgccaag ttgaaaccga
     4741 gaaaaaaagc gcggcaagtc cacgtctttt ttctctatcc agccttcagt ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagacac tttggaagcc atgcaaggct tgtatgaagg
     4861 gaaatatcta agctatccgc gaacggatac cccttacatt acggaagggg aatatgcgta
     4921 tctgttggat cacttagacg aatacaagca ttttttgagg gctgaagaaa tttcgactcc
     4981 tattcataca ccgaatagta ggtacgtgaa caacaagaaa gtccaggaac attacgcaat
     5041 cattcctaca aaaacagtta tgacagaagc gacgtatgaa agtctgacac ctttacaaca
     5101 agcgatttac gagcaagttt taaaaacgac agtggcgatg tttgcggaaa aatataccta
     5161 tgaagaaaca acgatcttga cgcaagtcca gcagcttcag ttaaaggcaa tcggaaaagt
     5221 accgatagat ctaggttgga agaagttatt tggcaaagag agcgacggaa aagagaagga
     5281 agaagagccg ctacttccta aagtgacaaa aggagaaatg gtgacggtag atctacaagt
     5341 ccttgagaaa gaaaccaagc caccacaacc ttataccgaa ggtacgttga tcactgcgat
     5401 gaaaacagct gggaaaaccg tggatagtga agaagcgcaa tccatattga aagaagtgga
     5461 aggaatcggt acagaagcga cacgggcgaa tattattgaa accttgaaac aaaaggaata
     5521 tatcaaggta gagaaaaata agcttgtggt gacgaataaa ggggttttgc tttgccaagc
     5581 tgtggaaaaa gagccgctgc ttacgagtgc cgaaatgacc gccaaatggg aaagctactt
     5641 gttaaagatt ggagaacgaa aaggaacaca aaccactttt ctagccaata tccaaaagtt
     5701 tgtctctcac ttattggaag tcgtcccagg acaaatccaa tccattgatt ttggatcgac
     5761 gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg
     5821 tccaaaatgc caggaacaag aagtcttact ctatcaaaag gtggcagctt gtacgtctga
     5881 agcctgtgat tttaaattat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggagatt attcaaaata gccgaacaag ccaaccagtg aaagggttaa aaggtcagaa
     6001 gggttcgttt gaagcaacga ttgttttaaa agaagacttc actacaggct ttgaattttc
     6061 tgaaaagaaa aaaacgaact tcaagaaaag aacacgacgc acgacaaaat aatggagggt
     6121 taatcttatg cgtaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagccaaaat
     6181 tcgttcagaa caagagaaat tacgtgccag taaaagaaga cttcagcaat taaagaacca
     6241 gcaacggatt caaaaagaaa tcgaattaga tcgatagaca ttaaccttgt tacaaataca
     6301 ctatcatgat atatttaatg tagtgtattt gtaacaaaag gggggaagta aatgcaagag
     6361 aaactgaaag aattgttggc tattcgcgat ggaaatttat ctatgaagga agccagaaaa
     6421 ttaggaatcg cagcaaccac tgtacaaaga ttggtgaatc aaggaaaatt gatcaaagtt
     6481 gatcgtggtt attatgtaga ggatggtcat ggaatagatg atttattttg gcttcagcaa
     6541 cgtttttcca ggggaatctt ctctcatgaa acgactttag atatttacca gctttctacg
     6601 aatatgccga aattcattca cttaactttt ccccatggat ataacgttga tcgttcaatt
     6661 acaaaagagc agcagttaca atttcatttc gtgaagaaag aagtttatga actaggaaaa
     6721 gtagagggaa cttcctttca aggaaatcct attactatgt atgataaaga gcgtacactt
     6781 tgtgatattt ggaatccgag atatgaaatt gaatatgaaa tgaaattaga agcattaaaa
     6841 gagtacatgg aagacccatt gagagatcca agtaaattaa gagattacat gacaaaacta
     6901 catgtagaaa aagaaatgaa ataccacatg caaagcttat attgatggag gaaaaagatg
     6961 acaccgacac aatttgcagc aaaaacaaga aatttagcaa agaggacagg attaaatgca
     7021 cagttagtac aacggcattt tatgatggat cgtttaattg aacaaatttc tgaatctaag
     7081 tataaaaatg atttcatttt gaaaggtggc tttttattag gatcaaaata tggaatcgat
     7141 agaaggacga caattgatat cgacacgaca tttagaaata gcaaattaac tgaagaaaaa
     7201 ttgaagagta tttttaatga tttaactagg aaacccacga aagaaggtat tgttttctct
     7261 attcaaggaa tgaaggaaac aagagaagcc gatttttatc ctggatttca aattaaattg
     7321 gtggctaatt tagagaaagt acgtgtgcca tttaaaatgg acgtgacaac aggagattct
     7381 atttatcctg aagtagatac acactatcac caattaatgt ttgaagataa aaaggtggag
     7441 attccagctt atccaacagc acaaattatt gcagaaaagc taagtgctac gttcagcttt
     7501 ggcacagata attcgagagc aaaagattac tacgacttat ttaccattcc gaaactagaa
     7561 aatatcgata cgaaagagtt atacaaaagt gttcgaaata catttacaaa acgtggggat
     7621 acaaaaccgt tgtcctttta ttatgaaaga gaaatggata agatcatagg aaatgactat
     7681 ttggaaaagc agtgggaaaa atatcgctca gttaacacgt ttgcgaattc catttcattt
     7741 acagatacag tcaacgaaat tgatcaccta atgaaaagtg tgatcaaaat tgaaaatcaa
     7801 gaaagaaaac ggcagtttaa aagatcacaa caaatgaatc aagaaatgga gagataaaga
     7861 tgatcaataa tgtaacttta gtaggacgct taaccaaaga tccagattta cgttataccg
     7921 cgagtggtac tgcagtagcg acttttaccc tagctgtcaa ccgaaatttc acgaatcaaa
     7981 acggcaatcg agaagctgat tttattaact gtgtgatttg gcgcaaacca gcagaaacaa
     8041 tggtgacatt agctaaaaaa ggtagcttga ttggtgtagt tgggcggatt caaacgcgga
     8101 cttatgacaa ccagcaagga caacgtgtgt atgtgacaga agttgtggcc gataatttcc
     8161 aactattaga aagcaaagca gcgacagaaa gccgtgcgca cgctgatcaa tcgtccacca
     8221 gtccaagcac caccactttt gaacaacggg atacagcgac gcctaataac aatggattga
     8281 acgctttaca gaatccattt gggaatccat ttggtggaca atcaatcgat attagcgatg
     8341 atgatctacc gttctaagta gcattttcta ttggttgcat ttgctacggt ttatgttata
     8401 atttagtcaa caaaaagaat tttcaatcta taaccaaaaa ccactagctg gcgggctagt
     8461 ggttttcttt tacgtttcta ttataagaat taacgggtag gcttactgtc tttcttggca
     8521 aatttacaat caagccattt gcagatatag tgcgcggcta cttctgccat gattgcaaaa
     8581 acaaaatcaa aaagaatttt caaacctata acctcctccc cttgccagat taggccgggg
     8641 tagcagacag caagactatt atagcataat tgaaatatcc aaatgattgg atttttgcta
     8701 taattttagc gaggtgattg tgatgaacaa atatgttggt ttgcttgata aaatccgagt
     8761 aataaaaacg caaccgttat tggttcgctt tactttacaa acgattcatg aatcgattaa
     8821 ttgtgtagtt gcagatattg aaattatcga taaattgcta attatggatg atggaaaata
     8881 caatattgcc gtcacaggtc acttcaataa acgaaatcag ctagttattg ctagcatgta
     8941 tgttagaaat cccgatcatt ttacgcgatc gatgggcata taaaaaaaga agagttgcaa
     9001 tcctaatgta ggactgcagc tctttttgtt ttacatagaa acaagaaaaa tgaaaaggcc
     9061 tttttaggaa ggcgcgagcg ccttctcaat tgaaaaacct ctctcactcc aaccgccacc
     9121 gccgttcgag aagaaaggaa gtgaacgttg cacgttcact aagataaagg gtcttgcaaa
     9181 gcaagacaaa atagaaaaaa cttcctctca ccaaaaggtt cgttcctgtt cgttatctct
     9241 ttattgattt gccagaccgg caacaagttg ccttttatca ccaaaaagcg acaaggacaa
     9301 gggtaaatgg cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattc attctggcca
     9361 acaaatcaat aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag aggaggcaga
     9421 atcaatcatg cgaaagaaat ttgattattg gggtgttccc ttcagcgaat tattcccaaa
     9481 ctatcatgca ccacatactg tcgaatgcga ttgtggacaa agagcgaaat gtatcaaaag
     9541 ctatcgtttg tatcaatgtc caacatgcgg aaaaaaatac accttgtctt atggcgatta
     9601 tattttgatt gatgagaaag gaaagaaacg ctgatgaaaa cataacataa attattgact
     9661 gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa agtagcagaa
     9721 gctttactta ttccgcggta ctggacgata gaaacccctt tagtagagga tgcaccgact
     9781 atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca agaaaacatg
     9841 gaaacttatg aaaacgaata ttggttaacc aaattagaga actaaaaagg agaaaaaaat
     9901 atggctcaaa attactatga cgaatttgtc aaattacctt tggataaaat ggcacaaaaa
     9961 atggaggata tgacattcct ataccatgag acacgtgttc caaaaaaaca ttataaggaa
    10021 agattgtcgg tagctgtgga agaaatgatt gaatcaggtg tcgaaatgaa tctcattgcg
    10081 acgtactatc gaacactgga agaactgaaa aaacaaaatg caaaatggtt tttccaagcc
    10141 ttactatgct tggaagtagg tgtcaaacca agcactatta aaccttctga atatcaagct
    10201 ttagaactaa cgtatgggaa atttgtcgaa acgaaaaaag ccaagactgt ttcaagtgaa
    10261 tggttagatt attttgaaaa tatcaataag tatggcgcct attacaccat gaaaaaggag
    10321 gacaacgaaa atgaataagg tgatttatca actaacaacg gaagatgtgt cacacttttt
    10381 aacaaacaaa gaacatttga atgaagtaat cagacggatt caaaaagact ttagttgtat
    10441 ggaagaaatg aagtacttta ttcaaagagt cgaagaacaa ttgtatttgg aaagtgaaac
    10501 gagtcaaacg aaggttacag tcagcaataa ttatttagac gtgtataaac atcgtcctga
    10561 aacggtaaaa aaaggaacgg agcttctacg actagaaaca gaaaaataca tcatgaatgt
    10621 ttacctagaa aggagtgaac tggtcgaaaa ctttgtcagc tgcacgattt gcaggaaaca
    10681 ctattactca gaatcagaat attattcaat ttttgagagc atggaattaa tggaagaaga
    10741 aatggaagag ctaaacagca aaaatgcgac gatagacgat tattatcttg tatttactag
    10801 atttttgacg tgtggaacgg aatctatcgt gttaatggga atggattatg aactcgctgt
    10861 acccgaagca tttttgaggg aatatgtaga agaagtagat aacccaggcg acgactggta
    10921 cacgacttta actgattttt tagataccta cacttacgac gatacagaag aaatccaaaa
    10981 tgaacttgac cgtaaacgaa tgccatatca acgatttgaa aggaaaaaaa ggcaataatc
    11041 atttataccg aaaagtgaga atgtgtgaaa taagaacaaa aaccttgaaa ttaagggaat
    11101 ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt gcgaaataag
    11161 cgggcaaagc ccgataacct aagagaaaga aggagatcca tatgaataaa atcattcctc
    11221 tgttgttcgt actgtgtatt ggcgagatac ttatgcgtca tggtgcagct gtacatttaa
    11281 acttcgtagt attcttagga atactagtat tttgtttgat tttttggcgc tttgtgttag
    11341 cccttgtgag tctactattc aagttgattg cagccatatt tttcataggg ttatttcgat
    11401 gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc gtgacattag
    11461 atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtacggaaaa agtgaatcta
    11521 agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga tttgatatac
    11581 cagcactaaa aaagtgacgt cacttttcat ttgaagaaac aaaagtgata gcacttaatt
    11641 gttcctacta tttagaatat tttctatgtc ctttttattc cattttgtat caataactat
    11701 gaaatcttta aagtatccag atcaattttt ttcttacctg attctaaaga ttttgttttc
    11761 ttaaacatat tatgatttcc tttcgactat tttcatataa atgttaacat agtttatgga
    11821 ttcatggaat gttaatctaa tttaatagaa aaggataatt aagtatatac gttttttctt
    11881 atgtatgtta tactatgtat atacagaaag aggtgtctac tatggaaatg ttacctataa
    11941 aaaaatgggg aaatagtaat ggccttagat tgccaaagta tattatggaa tatcttggaa
    12001 ttcatacaga agacaaagtg aagataatac aagaagaagt aaacggtcag aagcggctaa
    12061 ttatcgaagc ggctaatata gaaaatgaat taacaattga acagttattt gaaaattaca
    12121 ctgatgaaag aaatcatgtg acgattcaaa atttaggtga agcagtaggt aatgaaaaat
    12181 ggtgagaaaa cctagacaag gcgatattct attattgaat acagcacctc gatctggaca
    12241 cgaacagaca ggtaaaagac cttatattgt tttaagtcat gatattattg cggattatag
    12301 taacgttgtt acagttgctc caatttctag tacaactaga aactatccgc tatatgtaaa
    12361 tataaactca gaatatgaaa tgaagacaac aggaaaagta ttattagatc aactaacgac
    12421 aattgattat gaagccagag agtgtatttt tttggaaaaa gcgcatgata gtttagttga
    12481 ggagttattg atgaaagtta gaacagtttt tcaaaaagta agcaaataaa atagaaacag
    12541 cagcatgatc aactattcgt gattgaccat gctgtctttt tttattctat atacggtttg
    12601 tctcgtaaca ttgtagtctt t
//
LOCUS       DBNICI010000082        12618 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.082, whole genome shotgun sequence.
ACCESSION   DBNICI010000082 DBNICI010000000
VERSION     DBNICI010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.082"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            99..542
                     /locus_tag="AC1ZCC_001787"
     CDS             99..542
                     /locus_tag="AC1ZCC_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIQ1630035.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(548..1732)
                     /locus_tag="AC1ZCC_001788"
     CDS             complement(548..1732)
                     /locus_tag="AC1ZCC_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HIQ1630036.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            2000..2698
                     /locus_tag="AC1ZCC_001789"
     CDS             2000..2698
                     /locus_tag="AC1ZCC_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HIQ1630037.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2705..3382
                     /gene="nth"
                     /locus_tag="AC1ZCC_001790"
     CDS             2705..3382
                     /gene="nth"
                     /locus_tag="AC1ZCC_001790"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIQ1630038.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3508..4041)
                     /locus_tag="AC1ZCC_001791"
     CDS             complement(3508..4041)
                     /locus_tag="AC1ZCC_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630039.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4338..6719)
                     /locus_tag="AC1ZCC_001792"
     CDS             complement(4338..6719)
                     /locus_tag="AC1ZCC_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HIQ1630040.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6709..7356)
                     /gene="recU"
                     /locus_tag="AC1ZCC_001793"
     CDS             complement(6709..7356)
                     /gene="recU"
                     /locus_tag="AC1ZCC_001793"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HIQ1630041.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7420..7959
                     /locus_tag="AC1ZCC_001794"
     CDS             7420..7959
                     /locus_tag="AC1ZCC_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HIQ1630042.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8049..8468
                     /gene="gpsB"
                     /locus_tag="AC1ZCC_001795"
     CDS             8049..8468
                     /gene="gpsB"
                     /locus_tag="AC1ZCC_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HIQ1630043.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8490..8857
                     /gene="rnpB"
                     /locus_tag="AC1ZCC_001796"
     ncRNA           8490..8857
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="AC1ZCC_001796"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9066..10232
                     /locus_tag="AC1ZCC_001797"
     CDS             9066..10232
                     /locus_tag="AC1ZCC_001797"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing class I SAM-dependent
                     RNA methyltransferase"
                     /protein_id="HIQ1630044.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10278..11774
                     /locus_tag="AC1ZCC_001798"
     CDS             10278..11774
                     /locus_tag="AC1ZCC_001798"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HIQ1630045.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11855..12538
                     /locus_tag="AC1ZCC_001799"
     CDS             11855..12538
                     /locus_tag="AC1ZCC_001799"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIQ1630046.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         4238 a         2207 c         2601 g         3572 t
ORIGIN      
        1 tttcttggtt tcagattggg ttttcctaca aaatattttt atctttgttg aataggaaac
       61 aaaaaaacaa tataatgaaa gaaaaagaag gtgtagaaat ggataaaaga caactgctaa
      121 tagaagaata tctggattta gctaatgaga ttattcatcg taataaacca gaaatggaaa
      181 aagaatttgc aggatttact ctaaatgaac tagaagtgat tgaacatatc gggaaaatcc
      241 ctgatcctaa cgtgacaaaa ttagcgaatg ctagttacat gactagagga gccatcagta
      301 agctaacaaa aaaactgata gctagaggga tcataaacac ttatcaaagc gaagagaata
      361 aaaaagaaat ctattttcaa ttgacgccaa tcggacaaaa aataaggaat agacacgagc
      421 aactccatca aaaatgggta cagcgagatg ctactgtatt tgaaaatatg tcaaaagaag
      481 aatttgacac agtatttcgt tttatcggtc gatacagaga atttatacga agtagcaaat
      541 gaagctacta ttttttattg tagtgataat aggcaaaaat cataagcaaa aatccgaata
      601 ccgtcggaag aaatggtgct gcagcataaa gctgtcctgc gattagcggg ccgatgacgc
      661 gcgatagaga ttgtatactt tgggtaccgc ccattacaag accttgctgg ttatctgaga
      721 cagaattgga tagttggcca ttgaatacag gagtaaaaat agaatcaccg aaaccataac
      781 agataatccc aaataaaaac aaaatcggca gtagaagaat tccagataat gtgatgaata
      841 gataggcgag gatttcactg aacatcccta ttcttgtgat ctgctgttct gaaaattttt
      901 tgagcaatcg aggcataatg aacaattgcg agacagtatc caagattcca atcaaagaga
      961 agcttaaacc aaccaaccca gccttccact gaaaggcgtc aatcgaaaat tgagaaaaga
     1021 tagattgaag agagccagct gccaaccaaa cagcaaaacc tgctgtcaat aaaggaataa
     1081 cggatgaaat cttgaataat tgttgtaatt ggtgaaaagg tcttacatgg gagaaactta
     1141 aatctactga acgttttttt attggaaggc tttctagcat aaatgtgtag ccataaacgg
     1201 catttaagaa agtaaagagt gctccaataa atataggaac agaattcccc aactcagcta
     1261 atagaccccc tataatagga cccagcgttg ttcctatacc gactaaagca cccatccatc
     1321 caaaaacttt ggttctttca ttaggttccg tgatgtcggc aaagtaagca tataaggtgg
     1381 agatttctcc tgccgtcaat ccttcaatga tacgaccaag gaacaacatc catatcgaat
     1441 ttcctaaacc aaaaagcaaa tatccgatac ttgatcctaa taaactgata ataaggatcg
     1501 gatgacgccc aaaatgatca cttaagctac cgagaatggg ggcagataag aaggcagcta
     1561 gagcataaac agacatgagc aaagtgatat agaaagcttg ctgatgtatg gagtgagaat
     1621 aaggcagcgt taaaaacgga agtacaggac tgacgatagt aaatcctaat ccagtgaata
     1681 agacagagac gtatccaaaa actttagaat gtttatttgt gaaactgtac atgtaaaaac
     1741 tcctttatgc aataatgtta acttggaaac aaaaatagtg tatagtaatt ttgtttcctt
     1801 gtcaacattc taaggaacag cgaaatctac ttaatttatt tattccgaaa agtgaagata
     1861 cgcatcaaaa acatgaacaa acaaaaatca agtagacgac attgtaaatc tgaccgatca
     1921 gtgaagtaaa tagaaaagaa acctgttctt gcttcccatg ttttttttac gtatactgtg
     1981 attgagcgga ggagaaatca tgttgaattt agaaaattac ttaagcgctg gacaaacagt
     2041 catatctaat cttttactta aaaattatca taggatcggt ctacaatctg aagaattttt
     2101 atttgttcta cagttgcata tggcacagct tgaaggagat actttccctg atttgcagat
     2161 gattgctatg gatatgggaa tcaaacaaga tcagattttt caaatactgg atcgtctcgt
     2221 aacaaatgga tttatcaaga tagaaacgac gattgtcaac ggtaaaaaag cagatcgata
     2281 taatctttac cccatctatg atctattagg tgagtattta aaaacacaag aaaaaaaata
     2341 cgaagaaaaa acacaagaaa aagaaatcca gtcagtgtat caattatttg aacaagaatt
     2401 tggacgcccg ctttcttcga tcgagttcca gcggatcgga caatggctag aagaagacca
     2461 ttatcagccg gaaattttga agctagcact gcgggaagcc gtattaaatc aggcttatag
     2521 ttttaattac gttgaccgca ttttgctatc gtgggaaaga aaaaatctac gtacaaaaca
     2581 acaagtggaa gaagaccaaa aacgaagaaa acagcagctt cttcaaagag aagtagaaca
     2641 gtcagctcaa caagaagaat tgccgaaagt ctcactgaaa aattggttgg aggagtagcg
     2701 gaatatgtta agcaaacaaa aaacaatgga agctctagaa acgatgtatg gaatgtttcc
     2761 cgaagcacat ggagaactca agcacaataa tccatttgag ttattgattg cagtgatctt
     2821 aagtgcccag gcgacagatg tatcagtgaa taaagcaacg ccagatttgt ttgcctcttt
     2881 tccgacaccc gatgcgttag cagaagcttc aatcgatgaa atcattctta aaataaaaac
     2941 gattggtttg tatcgaaata aagcaaaaaa catcaaagcg tgtgcacagc agctgattga
     3001 acgtttcgat gggcaagtgc caacgtcaag agaagaattg atgtctcttc ctggtgtagg
     3061 cagaaagaca gcaaatgtcg tattggggga tgcattcggg ataccagcaa tcgcagtaga
     3121 tacccacgtg gaacgtgtct caaaacgcct gaggatctgc aaactagatg ctacggtcat
     3181 ggaagtagaa gaaacactga tgcggaaagt accacaagaa ttatgggtaa agacacatca
     3241 tacactgatt ttttttggta gatatcattg cacggcaaga aatcccaagt gcgaggtatg
     3301 tcctttgcta tcaatatgtc aagatgggaa aaatcggatg cgtctaaaag aaaaggcgct
     3361 aaaaaagaaa ccacgcctgt agacgtggtt tctttaattg caaataaatg tatgaattat
     3421 cttgttatta tggagaaaag ctgtttcgtt tatctttttt gcatgaaaaa gtaaatgtac
     3481 atactcagta gctaggatgg taacttatta caatttattg cgataaaaat aaaagttgtt
     3541 atttttattg ggaaaatacc cgatgtagaa aataatgcct tccaatatac ctataaaata
     3601 agtccagtat tccaaaggat tatataaata agtgacgtat ctatctaaag gtgtaataaa
     3661 aatgataagt gataataaaa aaataccatt tataactaaa cataaactaa tggatttggg
     3721 cggtttcagg ctcatcagaa aaatatagat tctggtgaac aacaaccata aaaaagtgag
     3781 aatcattgca tgagagcttc tttccataaa ttttccttta agagtagtgc ttaaaagtac
     3841 agaaaaaata ataaatgcaa ggataggagc aattaaagta aatttattta ataataaaca
     3901 aaatttttca gcttttttat tcttggaata acgaggtatt gttttcgata aaaaaagatg
     3961 gtgcagacaa attatgataa ttaagcatac tatcagataa ctcaaaatga agccttcttt
     4021 caaaaaatta ttcaaaacca tatattgtat tttttatttg ataataacaa tgggattatt
     4081 aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta taaacaagta
     4141 aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt taagctaaat
     4201 gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat acagcttggt
     4261 acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc acgcttgtag
     4321 acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg
     4381 tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat
     4441 gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc
     4501 tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta
     4561 gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag
     4621 ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct
     4681 ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt
     4741 gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac
     4801 gattctgaag tgacaggcgt cattttttta ttgtaacctg tccacacgga aatcgaataa
     4861 tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt
     4921 gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg
     4981 atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata
     5041 taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg
     5101 tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa
     5161 gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct
     5221 tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt
     5281 tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca
     5341 ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag
     5401 tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca
     5461 gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa
     5521 ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg
     5581 tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca
     5641 acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt
     5701 ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc
     5761 ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta
     5821 gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct
     5881 tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct
     5941 cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc
     6001 atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac
     6061 atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag
     6121 taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct
     6181 tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc
     6241 aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca
     6301 gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc
     6361 gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt
     6421 tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta
     6481 gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct
     6541 acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat
     6601 aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca
     6661 ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt
     6721 cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg
     6781 aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg
     6841 tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt
     6901 caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg
     6961 ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga
     7021 tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt
     7081 atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt
     7141 aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc
     7201 gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt
     7261 gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt
     7321 ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca
     7381 ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc
     7441 tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc
     7501 caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa
     7561 tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa
     7621 ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt
     7681 aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat
     7741 gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt
     7801 tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa
     7861 acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg
     7921 ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat
     7981 caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag
     8041 gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa
     8101 caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag
     8161 attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg
     8221 caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag
     8281 aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg
     8341 aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac
     8401 ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc
     8461 aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca
     8521 ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa
     8581 accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat
     8641 gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga
     8701 acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa
     8761 gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct
     8821 ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag
     8881 gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa
     8941 tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat
     9001 ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat
     9061 tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag
     9121 cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg
     9181 cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag
     9241 atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa
     9301 acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg
     9361 gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag
     9421 caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa
     9481 ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg
     9541 atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat
     9601 tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat
     9661 tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata
     9721 atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag
     9781 aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag
     9841 atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag
     9901 aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg
     9961 ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag
    10021 aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga
    10081 gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca
    10141 aaaaaagaaa attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc
    10201 gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat
    10261 gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc
    10321 ttctacaaca agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag
    10381 ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa
    10441 tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg
    10501 aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac
    10561 cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa
    10621 aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat
    10681 taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc
    10741 tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta
    10801 aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag
    10861 actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg
    10921 aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga
    10981 ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa
    11041 tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg
    11101 ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat
    11161 cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt
    11221 tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt
    11281 cattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc
    11341 tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg
    11401 ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa
    11461 atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc
    11521 cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa
    11581 tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag
    11641 agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac
    11701 atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat
    11761 atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt
    11821 tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga
    11881 ttatagtatg gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag
    11941 agttgtcaag ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac
    12001 gcttgcagca gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga
    12061 ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc
    12121 gatgattttt tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga
    12181 actatctctg gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat
    12241 cggcattttg cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga
    12301 actgtatccg caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa
    12361 tcatttttat gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga
    12421 agaagccaaa cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg
    12481 gtctattgac gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg
    12541 gaggttgtga cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac
    12601 ttctggaaca ctgtttac
//
LOCUS       DBNICI010000083        12573 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.083, whole genome shotgun sequence.
ACCESSION   DBNICI010000083 DBNICI010000000
VERSION     DBNICI010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.083"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(86..1051)
                     /locus_tag="AC1ZCC_001800"
     CDS             complement(86..1051)
                     /locus_tag="AC1ZCC_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HIQ1630047.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1285..2130
                     /locus_tag="AC1ZCC_001801"
     CDS             1285..2130
                     /locus_tag="AC1ZCC_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIQ1630048.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2321..3508
                     /locus_tag="AC1ZCC_001802"
     CDS             2321..3508
                     /locus_tag="AC1ZCC_001802"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIQ1630049.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            3514..3870
                     /locus_tag="AC1ZCC_001803"
     CDS             3514..3870
                     /locus_tag="AC1ZCC_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HIQ1630050.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            3872..4210
                     /locus_tag="AC1ZCC_001804"
     CDS             3872..4210
                     /locus_tag="AC1ZCC_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HIQ1630051.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    4286..4542
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4605..5639
                     /locus_tag="AC1ZCC_001805"
     CDS             4605..5639
                     /locus_tag="AC1ZCC_001805"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HIQ1630052.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            5632..6318
                     /locus_tag="AC1ZCC_001806"
     CDS             5632..6318
                     /locus_tag="AC1ZCC_001806"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HIQ1630053.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            6342..7190
                     /locus_tag="AC1ZCC_001807"
     CDS             6342..7190
                     /locus_tag="AC1ZCC_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIQ1630054.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            7387..8160
                     /gene="sufC"
                     /locus_tag="AC1ZCC_001808"
     CDS             7387..8160
                     /gene="sufC"
                     /locus_tag="AC1ZCC_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HIQ1630055.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            8173..9459
                     /gene="sufD"
                     /locus_tag="AC1ZCC_001809"
     CDS             8173..9459
                     /gene="sufD"
                     /locus_tag="AC1ZCC_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HIQ1630056.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            9456..10691
                     /locus_tag="AC1ZCC_001810"
     CDS             9456..10691
                     /locus_tag="AC1ZCC_001810"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HIQ1630057.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            10678..11148
                     /gene="sufU"
                     /locus_tag="AC1ZCC_001811"
     CDS             10678..11148
                     /gene="sufU"
                     /locus_tag="AC1ZCC_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HIQ1630058.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            11153..12544
                     /gene="sufB"
                     /locus_tag="AC1ZCC_001812"
     CDS             11153..12544
                     /gene="sufB"
                     /locus_tag="AC1ZCC_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HIQ1630059.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKCEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
BASE COUNT         4161 a         2350 c         2781 g         3281 t
ORIGIN      
        1 tacaaaaaca tgaacagctg tttttgtttg gccgttctta ttacttgatg cagaacagct
       61 ttttcacaac ctattgaatc gattattaag cgattttttt agaaacaaat tttccatgtt
      121 tgctatgttc tttaattaag aaacgtacgt aatgagcaag aacgtagaag acaactacac
      181 ttgcaaacag acattccgca acttttaaga aatccaatac gctactatag atacctaaac
      241 tgttgaagta atgaacagta gtaaatatag ctgttgcaga gataacaagc gggaatgtaa
      301 aggctgcata gcttggataa aaacgaagct tgaacaaacg tggaagcata gacagcacaa
      361 cgaaatacaa tacttgagat aaagcgaaca atgctacgac aagataaaaa ttaggttcag
      421 caaagttttt aagataacct gttaggcaaa gagaaccagg tgctgcaacg atcgtaatca
      481 gcggacgtgt cggatgagcc atatttctta cgaaaaagac acgtttgatg atgatcggca
      541 ataagaacgc atagcagatc aatgaaatcc aaaagaaaat ttttccaata agtgggaaga
      601 agttcccggc agttacagtg atgaccccaa atccaacgta aatgataaac cagcttggat
      661 aaattgcttc aattgaaaga tcagctttga taacatggtg ataattgaaa taaaagacaa
      721 ggatcacttg gaagataaca gcgcacagcc aaatgaattt gacaattggt gcaatacttt
      781 catatgaaac aaaataagta caaataacca tgagtgacat cgtaaaagtt ggtgaaacgg
      841 aggcgatgat tggatcatgc aacgtttgtt tcgtgtgttc gaataagata aacaatttaa
      901 caaaaatgaa tatcataaga atcatagaga taccgccagt tagattgcct aaagcaacat
      961 ggtgatagtc tttaaacaga tttccaaggg aagctaaccc aaggatcaac ccgcagatcg
     1021 ggatcggaat aattttcaaa aagttcttca tatataatat tctccttttt atttaataga
     1081 ttttagtacc aagatgaacg aatacattgt aaaatgaata ggaatgtatg attacgagca
     1141 agcagacagc tgattgtctt catctaagtt actagttgga tcagcaactg ttttgttgta
     1201 catttctatt atagatggtt ttttgtatat ttaaatcaaa tagtttttat agtaattatc
     1261 ataaaaagtt atggaagtgg tgccatgttt aagttattaa cgacttttcg agcagtatat
     1321 gaaacaagaa atttttccag agcagcagaa gtactgtttt tatctcaacc agcagtctcg
     1381 aatcaaatca aacagttgga gcgggaattg aatatcgatc tttttcaacg taacggaaga
     1441 aaagagatgc ttccaactaa gcaagccgac ttattatatg aacggacact ggttttgtta
     1501 gaagaatggg aagaaactca gcaaaaattg ctcaatgaac aaaatgaaga agaagtatgt
     1561 cggatcgttg cttcccacac ctttgctgtc tatcttttgc ctcagttgat gaaacatctg
     1621 atcaaaaggt ttcctaaagt gcattttgag atcgaaatca cgaattctca tgaatcatta
     1681 gaacaagtag cgaaacatga atccgatttt ggttttatcg aaaagccatt agagacttcc
     1741 ggcgtacaaa gatactcttt gatggaagac cagctagtca tcgctgggga cccaaaaagc
     1801 aaattgtggc ttgtaagaga agcaaactct ggtgtttttc attacacgca gcgttatttt
     1861 gaagagatga atatccaaga taagaagatg atcgtgaaaa gtaacgatgt aattgttgcg
     1921 ttgctgaagg aaggaatcgg tcgctcgatt ttgtctaagc gctctgttcc agaaaatgtg
     1981 ccaatgactc cgttgaattt ccatcgatat ttttatttca tccaacgaac caatctggtt
     2041 tctgatcagc tgctagagat tgccgaagag atccttcgat acaatgaaag cacagaaact
     2101 tctcacagcg aggagaatct cagctggtag ataattgcat gtatcaaaaa aagttctatg
     2161 atacactaaa gataagcagt aggcttttgt actgcatttg ctcaagcaac tgcacaaacg
     2221 gaaagttaat ttttcccaaa tgtctatgga aagactttcc attatctaac tggaccgcta
     2281 taataatcag cagtatggtt tttaagaaaa gggatgaaac atggaaaaaa aattaaaagc
     2341 ttcgaacgat aatcgtattg attatggcgt gattttacca gtctttctgt tatgtttgat
     2401 tggtctgctc tcattatatg tagcacttag ccatgatccg aacaatccag cagtcttgaa
     2461 aggcgtaggg atgcaagttg tctggtattt agttggagcg gcagcaatcg ttgtcatcat
     2521 gcatttgaat tccaaatgga tctggaaact gactccttac ctttatggtt tggggttagt
     2581 ggtaatggga ctcttgctca aattctatga tcagaattta gctgcttcaa cagggtctaa
     2641 aaactggttt cgattcggtg cgtttacttt ccagccagca gagttgatga agattgcgta
     2701 catcttgatg atggcactga tcgtaactaa acataatacg caagtaaaag aacggacgat
     2761 gaaatcggac ttttggctga ttgggaagct cttgatcgtg acaataccgg ttttagcatt
     2821 gatcatggcc caagatgact ttggaacgat gcttgtcttt cttgcgatct ttggcgggat
     2881 tttcctgatg tctggaatct cttggcgcat catcgcacct gtagtaatct tggcagtagt
     2941 cgttggagca ggaacgatct tcttggttac gactgaaggc ggaagagact tgctttataa
     3001 agttggattc aaatcttatc agtttgcacg tattgattca tggctggacc ctttccatga
     3061 tacgtcagga atgagttatc agccggccca aggacttttg gcaattggta ccggtggctt
     3121 gtttggtaaa ggattcaacg taagcaatat ctatgtgcct gttcgtgagt ccgatatgat
     3181 ttttaccgtt atcggtgaaa acttcggctt tatcggtggt gcattcgtca ttttcttgta
     3241 ttttatttta atctatcgta tgattcgggt gtgtttcgat acgaataatg aattttacgc
     3301 ctatatcgca agcggtttga tcatgatgct gctgttccat gtctttgaga atattggagc
     3361 aaatatcgga ttacttcctt taacagggat cccattgcct ttcattagtc agggaggatc
     3421 ctcgatttta gggaacatga tcggtatcgg gttgatatta tcgatgagat accaaaatga
     3481 agcacctgca agatcttcaa ggaggtaaga aaattgtaca ccttttactg gtacccgaaa
     3541 tgttcaacat gcaaaaaagc caaagcgtgg ttagaccagc atggcgttag ttatcaaacc
     3601 gttgatatga tcaaagaagt accgaaggcc tcaacgattg agacttggct agaaacgagt
     3661 caagtaccaa tccgtcgcct gttcaacaca agcgggatga aatatcggga acttggactg
     3721 aaagatcaag taggcgagtt tacggcttca aaagcgagcc aagtcttatc gacagatggt
     3781 atgctgatca agcgaccact gatcttacga gaggaccagc ttgttgcaat tggattcaat
     3841 gaaaaaattt atgaaggaga actgatataa aatggcaggc aagcatttga agaaaaagga
     3901 caacctttgg atcttattta atggaaaaga atactgtgtt ggtttgacaa atgaagcgca
     3961 agaagaactt ggagacatca cgtttgccaa tctgccaaaa gcaggacaat cgctaaaaaa
     4021 agatgaacct ctgatcgaag ttgaagcgga aaaagcggtc aatgaatttg ccagcccgct
     4081 aacgggtaca gtttcttctg ttaatgataa aatcggtgaa gatatcaata tcttgaatga
     4141 ttcagatgaa atgaatgcgt ggatcttaag tttcaaagat gttgatccaa aagaatttga
     4201 cgaattataa aatcagaaaa tcacttgaaa aaagttgaca ctgattcttc cagttgctat
     4261 aatcataagc aatcctatat gaaacgagaa agctttgaaa agaagagtac atgtcacgga
     4321 cgtttgaaga gagccctgtt tgctgaaaat gggtaacgaa aatgagatgg aagatggtct
     4381 tagagcagag taagcgagcg taagcaaact tactacggga gtgcccgtta cagcgcgaca
     4441 gtatacggaa agaagctaag tccaagtact ggaagaggct gttattgcga agtaacggta
     4501 aatcaaggtg gtaacacgga agttcaagct ttcgtccttt tgcttaaggc aagagggcga
     4561 aggctttttt gtttgaaaaa ttataagaga aagatgtgaa tggcatgtca ttaatccaac
     4621 taacgaatgt caaaaagaca ttcaatggga aaaacggaaa catccaagcc gtcaatgacg
     4681 tttcactaaa cgttgaaaaa ggggatattt atggaatcgt cggctattct ggcgcaggaa
     4741 aaagtacatt agtccgtctc ctgaatggac ttgagcttcc cacgagcggc gaagtgatcg
     4801 taaacgatca agatattaca aaattaaaaa acaaagaatt acgtacattc cgaaagaaaa
     4861 tcggtatgat cttccagcat ttcaacctgt tatggtcaag aactgtactt gaaaatatcc
     4921 agctgccgct ggaattagct ggcgtaccaa agagcaaacg taaagaacgg gcagaagaac
     4981 tgttgcgttt agtcgggtta gaaggacgcg gacaagcata tccttcacaa ctttccggcg
     5041 gacagaaaca acgcgtcggt atcgcacgag cattggcaaa tgatcctgag atcctgcttt
     5101 gtgatgaggc tactagcgca ttggacccgc aaacaacaga agaagtatta gacttattgt
     5161 tagcgatcaa taagaaattg aatttgacca tcgtcttgat cacacatgaa atgaatgtga
     5221 tccgcaaaat ctgcaacaaa gtcgctgtga tggagttggg gaaagtcgta gaagaaggag
     5281 acgtattgac tgtcttcaga caaccgaaaa aagaagtaac caaacgcttt gtacaacaag
     5341 acatcgaacc agaagctgat tcgacagaac tattagctga actcatcaaa gaaaatccaa
     5401 gcggccgttt ggcaactttg acctttagcg aaagaaatgc caacgagcca gtcatttccc
     5461 aagcgattcg acgctataac atcgatgtca acgttgtgta tggaaagatc aaacaagtga
     5521 aagaaggttc ctttggttca ttgactgtct tgatgactgg tgaaaccaaa gaattagatc
     5581 aagcagaagt gttcatcaaa gaacaaggag taggaattga ggtgatccat cgtggataaa
     5641 agcttttcag aaacatatct agattttaaa caaatcaacc cggaaacgat gaatcaggct
     5701 atccttgata cactatatat gacattaatc tcaatggtct tagtgacgat catcggatta
     5761 gttttgggat tgatccttta ctctattgga cgcaaaaaca aaccgtatgc tcgaatcatt
     5821 tatggaatcg tatcgatcat cagtaatatt ttccggtcaa cgccatttat gatcctgatg
     5881 gttttgatca ttccatttac taaagcaatc gtaggcacta tgctaggtgc gcaagcagcc
     5941 attccagctc ttgttttatc cgcagcacca ttttatgccc gtctagtaga gattgctttt
     6001 cgtgaagtca gccctggtgt actggaagca gcagatgcaa tgggagcaag ttactgggaa
     6061 atcatctgga aggtcttgat acctgaaagt attcccgcat tgatttctgg attaacagtt
     6121 acaacgatct cgatgatcgg atttactgcg atggccggcg cgatcggcgc aggtggactt
     6181 ggaggtcttg cttggcagga aggctaccag cgtggtaatc taacagtgac attcgttgcg
     6241 actttaatta ttttactaat cgtatttatc gttcaaggaa tcggtgattt cttaactaag
     6301 aaaacagaca aaagataata ggatataggg ggatgtagga tatgaaaaag aaaatttttg
     6361 gatttgcagc agcgcttttg ctgactgtag gattagcagc atgcggtaac aacagcgaca
     6421 gcaaagattc agcaaataaa gcagacgaca cgacattgaa agtcggcgct tcaccgacac
     6481 cgcacgcaga gattttggaa catgtgaaac cattattgaa agatgaagga atcgatcttg
     6541 aaatcgtcaa attcgatgac tatgtattac cgaaccaatc tttggaagaa ggcgacattg
     6601 atgccaatta cttccagcat attccttatt tgaacaaaga aatcaaagaa aaaggatatg
     6661 actttgtcaa tgccggtgct gtccacatcg aaccaatggg cttgtactct aaaaaagtca
     6721 aagatgtttc cgaattaaaa gatggtgcaa caatcattac ttcaaattct gaatcagact
     6781 ggggacgcat catcacaatt ctacaagatg ctggcttagt caaagtcaaa gatggagtag
     6841 atctggaaac agcaacattc gatgatattg aagaaaaccc taagaacttg aagttcaacc
     6901 atacgatcga cccatcactt ttagcttcta cgtaccaaaa tgatgaaggt gacctagttg
     6961 caatcaacgc aaacttcgct tacggcgcag ggttgaatcc attagaagat gccgtattgc
     7021 ttgaaaagga caactctcct tacgtgaata ttattgcgac tcgtaaaggt gatgaagaca
     7081 gtgacaaaat caaaaaactg attgaagtgc ttcaccgcaa agaagtccaa gaatggatca
     7141 cagacaaatg gggcggctct gttaaaccag taaaggctga tgcaaaataa ggtatggaac
     7201 aaaaaagatg agaaataagt ctgtaatggc ttatttctca tcttttttat acctattcta
     7261 atttagaaat caaaattgtt taataataaa taaaagatat ttgattttag taaggaaggt
     7321 cattgcgctt cattctcaat tagtctaaaa tgagtgagaa cgaaagagaa tttgggggta
     7381 taatgcatgt ctgtcttaga aataaaaaat ctacatgtgt cgatcgagga aaaagaaatc
     7441 cttaaaggcg taaacctaac aatgaaaacc ggagaaatcc atgccatcat gggacctaac
     7501 ggcacaggaa aatctacctt atctgccgct atcatgggca atccaaacta tgaagtaaca
     7561 gaaggcgaaa ttttgtttga cgggaaaaat gttttagact tggaagtaga tgaacgtgcg
     7621 cgacttggct tatttttagc tatgcaatat ccaagtgaga taccaggtat cacgaatgcg
     7681 gaattcatgc gtgcagcgat caatgcgaaa cgtgatgaaa acgacaaaat ttctgttatg
     7741 gcatttttga aaaagttaga tgaaaaaatg gctttactaa atatgccaga agaaatggct
     7801 gaacgttatc tgaacgaagg tttttctggc ggagaaaaga aacggaatga gatcctgcaa
     7861 ttgttgatgt tagaacctac atttgcaatt ttagacgaaa tcgattcagg tctggatatc
     7921 gatgctttga aagtcgttgc taaaggagta aatgaaatgc gcggagacaa tttcggtgca
     7981 ttgatcatca cccactatca acgattattg aactatatca cacctgatgt cgttcacatc
     8041 atgatggaag gccgagtagt catgacaggg aatgctgacc tagctaaacg tctggaagca
     8101 gaaggctacg ctggtatcag taaagaatta ggaatcgact ataaagaaga agaagcgtaa
     8161 gggggagcaa gaatgaaaga aaaaaactgg ctcgattatc ttgacgcggt aaatgatttt
     8221 tctttaagta aaggcgaacc tgattggatg agaaccttcc gtcaagacgc actagcgaaa
     8281 gcagatgaac tgccattgcc gcatatcgat cgggtgaaat tccatcgctg gtcgttattt
     8341 gatgtgaaag aaacacaaac catcagtgaa acaggcacga ttccggcatt tgatgcaatg
     8401 aaagacaatc ctgtgcttgt ccaacaaggt tcatggacga tttttgaaca actgcctgtg
     8461 gaattagcag aaaaaggagt catttttacc gacttgttca ctgcaatgat cgaatatcca
     8521 gaactagtcc aagaatatta tatgaaaaaa gcagtaaaca tgaacgaaga ccaattgact
     8581 gcactccatg tagcttttat gaacagcggt attttcctgt atgtgcctaa aaatgtcgtg
     8641 atcgatgaac cactcgagtc cttattcatt caagatggcg catccgatga gcacttcttc
     8701 aagcatgtgt tgatcgtggc agatgaacat agtgaatttt cttatctgga acgtttccag
     8761 acaaccaaag aacaagtagc aaaatcatca ggtaatatca tcgtggaagt catcgctaaa
     8821 gctggttcaa aaatcaaata ttcagccgta gatcaattag gagaaaatat tacttcttat
     8881 atgaatcgcc gtgggcacat tttacgagat gcttctgtcg actgggcaat tggtgtcatg
     8941 aatgacggtc atgtgatcgc tgactttgac tcagatttag caggagaagg tgcacacgca
     9001 gaagtcaaaa tcgtggcaat cagctcgggc agacaaatcc aaggaatcga tacacgagta
     9061 accaataaag cccctcatac gatcgggcat attttgcaac acggagtgat ccgagaaaaa
     9121 ggaacattga cctttaatgg tatcggccac atcttgaaag gggcaaaagg tgcagatgcc
     9181 caacaagaaa gtcgtgtctt gatgctttct gataaagcac gtggtgatgc gaatcctatt
     9241 cttttgatcg atgaaaacga agtaactgct ggacatgctg ccagtgtagg acgagttgat
     9301 ccagaagaaa tgtactatct gatgagtaga ggattgcata aagaagaagc agagcgctta
     9361 gtgattcgag gattcctcgg ttctgtctta acagcaatcc ctgtagaaca agttcggaaa
     9421 gaattagtcg aagtaatcga agggaagttg aatggatgat cgatgctaaa aagctacgca
     9481 atgattttcc tatcttattt caagacgtca atgatgaacc tttagtttat ctggataatg
     9541 cagcgacgac ccaaaagcca aaacaagtac tccaggcatt agcgtactat tacgaacacg
     9601 ataacgccaa tgttcatcgc ggtgtccata cgttagcaga acgcgcgacc catgattatg
     9661 aacaagcaag agaaaaagta cgggcgttca tccatgcaaa agaaacagca gaagtgctgt
     9721 ttactcgtgg tacgacgaca agcttgaatt gggtagccag aaccttcggc gaccgctata
     9781 tcaaagcagg agacgagata gtgctctctt acatggaaca tcattctaat gttatcccgt
     9841 ggcagcagtt ggcaaaacga acaggtgctg tcttgaaata tatcgaacta acagaagaag
     9901 gcttcctgga tatggaggat gcagcgaaaa agattacgga caaaaccaaa atcgtctcca
     9961 ttgctcatgt atccaatgtc ttaggtgtct tcaatcctgt tcaagaatta gctgaacttg
    10021 tccatgaaaa cggcggggtc atcgtagtag atggtgctca atctgccccg cacatggcca
    10081 ttgatgtcca agcactagac tgtgatttct ttgctttcag cggacacaaa atgtgcggac
    10141 caacaggtat tggtgtttta tatggtaaac gaaaatggtt agaagaaatg gaacctgttg
    10201 aatttggtgg cgaaatgatc gattttgtcg aattatacga cagtacatgg aaagaactac
    10261 cttggaaatt cgaagcaggg acaccaaata tcgccggcgc tatcgcatta ggccatgcaa
    10321 tcgattattt gcaaaaactc ggtatggaca atatccatcg atatgaagaa gaactagccg
    10381 cttatgtgtt gccaaaatta caagcaattg atggattgac tgtttatggt ccacaagacc
    10441 cgaattttcg gacaggagtc gttgcattca atttggatgg gttacatccc catgatgtcg
    10501 cgacagcttt agacatggaa ggggtagccg tcagagcagg tcatcattgt gcgcaaccgc
    10561 tattgaagta ccttgaagta tcagcgactg ctcgcgcgag cttttatttc tacaacacaa
    10621 cgcaagacgc cgaccgttta gttgaagcaa tcatggcaac aaaggagttt ttccaacatg
    10681 ggactatcta aattagacaa tttataccgt caagtgatct tggatcattc ttcccatccg
    10741 catcatcgtg gaagcttgac tgactcgaca agcaagatcg aattgaacaa ccccacatgt
    10801 ggagatgtga tcgaattgca agtcgatgta aaagacggaa tcatcaaaaa tatcgccttt
    10861 gacggcagtg gctgctccat cagtacggca agtgcctcga tgatgacaga cgccgtcatc
    10921 ggaaaatcaa tagaagaagc agaacattta actgtagact ttctacagct tgtccaagga
    10981 aaagaagtca aagatcccga ccaattaggc gatgctgcta tgcttggtgg tgtagctaaa
    11041 tttcccgctc ggatcaaatg tgcgacactt gcctggaaag cactaggaaa agcaatggaa
    11101 aacgatggta ccgcagcttt ccatcattac cacaaggaag aggagtgaga acatgggcgt
    11161 accagaatta gaagaatata aattcgggtt tcacgatgat gtcgaaccgg ttttcagtac
    11221 aggagaaggc ttgacagaag aagtgatccg tgagatgtca agaatcaaat gcgaaccaga
    11281 atggatgctt gaattccgtc tgaaatctct agaaacattt aacaaaatgc caatgcaaac
    11341 atggggcccc gacctttcag acatcgattt cgatgccatc aaatattacc aaaaaccaag
    11401 tgacaaaccc gctcgtgact gggaagacgt accagaaaaa atcaaagata catttgaaaa
    11461 gattggtata ccagaagctg aacgagctta tctagccggt gcttccgcac aatatgaatc
    11521 agaagtcgtg taccataata tgaaagatga attcgaaaag ttaggaatcg tctttactga
    11581 cactgactct gctttgaaag aataccctga attattcaaa gagtactttt caaaacttgt
    11641 accgccaacc gacaacaaat tagcagcgct gaattcagcc gtatggtcag gtgggacctt
    11701 tatttacgtg ccaaaaggcg tcaaagtcga tgttccatta caaacgtact tccggatcaa
    11761 tgcggaaaac acggggcaat tcgaacgtac gctgatcatt gttgacgaag gagcaagcat
    11821 ccactatgtc gaaggttgta cggcacctac ttattcaagt aacagcctgc atgccgccat
    11881 cgtagaaatc tttacaagaa aagatgcgta ttgccgttac acaacgatcc aaaactggtc
    11941 agacaatgtg tacaacttag taaccaaacg agcaaaagca tacgaaggcg caaccgttga
    12001 atggatcgac ggaaacctag gtgcaaaaac aacgatgaaa tacccaagtg tttacttaga
    12061 tggaaaaggt gcacgaggca ccatgctctc catcgccttt gcaggagcaa accaaatcca
    12121 agatacgggt gcaaaaatga tccacaatgc accaaataca tctagctcta tcgtctctaa
    12181 atcaatttca aaagacggcg gagaagtaaa ctatcgagga caagtaacct ttgccaaaaa
    12241 cagtgcgggt tcgatctctc atatcgaatg tgacacgatc atcatggacg aatggtcgaa
    12301 atccgatacg atcccattca atgagatcca caacagccaa gtctctttag aacacgaagc
    12361 caaagtctca aaaatctcag aagaacaact ctactatctg atgagccgag gcctgaccga
    12421 atctgaagca actgaaatga tcgtcatggg ctttgttgaa ccattcacca aagaacttcc
    12481 aatggaatat gcagtcgaac tcaatcgatt gatcagttat gagatggaag ggtctgtagg
    12541 ttaataacta tttcattgct cgaaacgttg ata
//
LOCUS       DBNICI010000084        12429 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.084, whole genome shotgun sequence.
ACCESSION   DBNICI010000084 DBNICI010000000
VERSION     DBNICI010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.084"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..187)
                     /locus_tag="AC1ZCC_001813"
     CDS             complement(<1..187)
                     /locus_tag="AC1ZCC_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630060.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPG"
     gene            complement(197..1012)
                     /locus_tag="AC1ZCC_001814"
     CDS             complement(197..1012)
                     /locus_tag="AC1ZCC_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1630061.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1274..1717)
                     /locus_tag="AC1ZCC_001815"
     CDS             complement(1274..1717)
                     /locus_tag="AC1ZCC_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HIQ1630062.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(1851..2189)
                     /locus_tag="AC1ZCC_001816"
     CDS             complement(1851..2189)
                     /locus_tag="AC1ZCC_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIQ1630063.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2177..2554)
                     /locus_tag="AC1ZCC_001817"
     CDS             complement(2177..2554)
                     /locus_tag="AC1ZCC_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIQ1630064.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2778..3971
                     /locus_tag="AC1ZCC_001818"
     CDS             2778..3971
                     /locus_tag="AC1ZCC_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIQ1630065.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4137..4559)
                     /locus_tag="AC1ZCC_001819"
     CDS             complement(4137..4559)
                     /locus_tag="AC1ZCC_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1630066.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            4954..5148
                     /locus_tag="AC1ZCC_001820"
     CDS             4954..5148
                     /locus_tag="AC1ZCC_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630067.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            5145..5591
                     /locus_tag="AC1ZCC_001821"
     CDS             5145..5591
                     /locus_tag="AC1ZCC_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630068.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLS"
     gene            complement(5801..6973)
                     /locus_tag="AC1ZCC_001822"
     CDS             complement(5801..6973)
                     /locus_tag="AC1ZCC_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HIQ1630069.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(7179..8213)
                     /locus_tag="AC1ZCC_001823"
     CDS             complement(7179..8213)
                     /locus_tag="AC1ZCC_001823"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HIQ1630070.1"
                     /translation="MRLCRNTKARCRNVAKYRVKLTLDPDKPLDTIHLYAKNVIKESN
                     IKIQKAGNFSGVDKLAGAQFQIKNSENQFVTGFDENTGVALYDPAESALTFTTDTEGS
                     ISVNGLIDGNYTLVETKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNP
                     NGVVNRENTTTDNTITVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMK
                     KIANLASGEYPYAWSDEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYA
                     LPKNEAAYTEFTVDDDPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIY
                     FKKRHQETEA"
     gene            complement(8144..8590)
                     /locus_tag="AC1ZCC_001824"
     CDS             complement(8144..8590)
                     /locus_tag="AC1ZCC_001824"
                     /inference="COORDINATES: protein motif:HMM:NF027869.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HIQ1630071.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(8587..9102)
                     /locus_tag="AC1ZCC_001825"
     CDS             complement(8587..9102)
                     /locus_tag="AC1ZCC_001825"
                     /inference="COORDINATES: protein motif:HMM:NF037639.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HIQ1630072.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            complement(9175..10089)
                     /locus_tag="AC1ZCC_001826"
                     /pseudo
     CDS             complement(9175..10089)
                     /locus_tag="AC1ZCC_001826"
                     /inference="COORDINATES: protein motif:HMM:NF027869.6"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(10086..12413)
                     /locus_tag="AC1ZCC_001827"
     CDS             complement(10086..12413)
                     /locus_tag="AC1ZCC_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HIQ1630073.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         3513 a         2429 c         2271 g         4216 t
ORIGIN      
        1 aacctggacc aataaaaagt aaagaaaaaa taaaggaaaa gacattcctt ccatctatcg
       61 acaaaaggaa cgcagctgct atatacaaga tattgacaca ccaaaaacaa aggctacttg
      121 ccaatgattg atacaaccaa taaaagaatt ccatataaac atttttgtca aaacgctgct
      181 ctttcataat cacctcctat tccttcattc ctgatgaagc gatcccttct acaaaatatt
      241 tttgcatcgc taaaaatata aaagcaacag gaagaacaga aacaactgag gcagccatga
      301 ttaatgcgta ctctgcatca aatagcccaa taaacatttt caaccctaac tggattgttt
      361 tattttccgt agaagacaaa taaatgaatg gccccatata atcgttccat gtattaacga
      421 atgtgatgat cgtcaaactg gcaatcgccg gttttgtaag aggtaaaatg atcttgcgat
      481 agattcccca ctctgataag ccatcgatcc gcgcactctc acataaagaa tctgggatac
      541 tactgtagta ttgcttcaac aagaacacac caaaagcatt gaacgcttgt aatagcacca
      601 acgaccacaa cgtatatgtt aaacccactt ttctcatcat aatgaactgc gggatcatat
      661 aagactgcca agggacagca atcgtgccga tatatgctaa aaataaaata tcgcgccctt
      721 taaaatttag tttagaaaac ccgtaagcag caaaactaga agtaaacaac tgaatcaacg
      781 tgatcacaac gcttaaaaaa gctgtattct taaaaaatgt cactaaagga atacgagacc
      841 aaataaccag ataattctct aaatgccaag ttttagggat ccattgaatc ggaatcgtga
      901 acacttcatt atttgctttg aatgaagcag aaaccatcca taaaaaagga atgatcgtca
      961 ataaagctaa tacaatcagc agaatcatac ccaaccattg aataaagggc atgttttttg
     1021 aacgaatttt tgcttttttt ctttcatttc gaacacctcc aaagttataa tcttcatttc
     1081 tttgtaatga ttacaatcca gaacaacggt tattatagct gattcatttc cgttcttctt
     1141 atgtttgtaa gcgcttattt aacaagttat gtaaatctct ttattttttt tgatttaaaa
     1201 atgaaaaaat acaaaagcaa gactgtcata cgtctcgctt ttgtattgaa atttcttcga
     1261 ttagagatac ttattactgt tcaaaagctt ctttgaaacg gtgaatcaat tcattgacta
     1321 caggtgataa aatccgattc tttttccaga ctagcatgca gttcgtttca atcattgggt
     1381 caagcggaac gaatgtcact cgctttgaaa gatgacttac agcaccttct atgactaaag
     1441 cagcacctac ctgattctca attagcgcaa atacattgaa aatcaaattg tacgttcctg
     1501 tgatcagcaa ctgctccttt tttatgccta accagtcagc aaacattcgc tgtatttctg
     1561 gacgctttga gatcaaaaga ggtacattcg caaagtcttc ttttgttatc acgtcttttt
     1621 ttgctaaaac agaattttct aaaatgagca atccccattt ttcctttctt ggtaacaaaa
     1681 tcgtttcgta tttctctgta tctactggat ctagcaaaat cgctaaatct aacaggcttt
     1741 tttctagtca ctccgtgata tcatcacttg ttccgctgtg gatatgaaat tgaacgctgg
     1801 gataatcacg gatcaacttt tctagtaata gtgccaatgt attcgaatta tcagcttcca
     1861 cacagccgat cgtaaatcgg ccgctgaata attgtttttt tctgttctcg aattcttgag
     1921 tggtttgatt cgttaaatct aaaatttctt ctgctcggct tttttagaaa ataacctgct
     1981 tctgtcaaga tcattttgcg gttttcgcgt acaaacagtt ctgttcctag ttcctcttct
     2041 aactccttca gttgtcggct taaggttggt tgtgtaagat gtagaacttc tgcggcttga
     2101 gagattgttc cttcttctgc aattgtccaa aaatatttta gtaagcgtaa ctccatagcg
     2161 atgctccttg atttttttac ttgatacatt ccattttatt tctagataac acacgatcat
     2221 atttcttgat ttttccttgt aaacgttcca aacactcttg catttcagca atttctttcg
     2281 tcaaaagttc actttcttca atcaaaagct tacgccgctc aaatacagtt gcttctcctt
     2341 cgctagatag gcgaatatat tcaatcaaag actcgatcga taatccggca tttcgcatac
     2401 agatcgtaaa gtcgacccat tttaggtctt cttctgtata atcacggatt ccgccttttg
     2461 tccgattaac tggtggaatc aacccgattc gttcataata gcgcaatgta tcggccgaaa
     2521 tgcccttttc ttcactgact tgtttgatat tcatctgatc actctttctc ttaatttctt
     2581 gccactttac ttatgtattt tatttattct acaactttaa gtgtactcca agtcaatact
     2641 catttctatt aaaactaagc tggattatct atatcggttg tttgtgtatt atttcaaata
     2701 aaaaagtctt gtttttttat tcttttttgg atatactttt aacaatttag ggaaatttta
     2761 ggaaaagggg tataaaaatg acaacgtatc agcgaaaggt attattatcg acatcggcgg
     2821 gaattgcttt agagaatatg gatattatgt ttttagcttt ttcattatct tcaatgatta
     2881 cttcttttca tattagtggt acgcaagcgg gtttcatttc aacgatcact aatttgggga
     2941 tgctcattgg aggtattttt tttggcatca tagctgataa atatggacgt gtgaaagtat
     3001 tttctcaaac ggtccttctg ttttcgattg cttctttatt gatgtatttt gcttcaaaca
     3061 tctacttggt ttatctcttt cgctttatcg caggtgtagg agcgggagga gaatacgggg
     3121 cgtgtatgtc cttgatttcc gaaagttttt caaaaaaaca aattggacga gcttcttcgg
     3181 ttgctggtat tggtgcgcag gtcggtgcgg ctttggctgc gattttagct gctgtgatca
     3241 ttccaacgtt tggttggaag atgttgtatg tcgtaggtgt actgccggtg ttgatggtct
     3301 tgttcattcg tcgcggcttg actgaaccaa aggaattcaa atctgtacaa tctgccgaaa
     3361 agaaaaccaa acttagcaga ctgtttgaat caaaacatct tgcttggcaa acatgtggcc
     3421 ttagtatcat ggtaactgtc caaattgccg gctattttgg gttaatgaat tggctgcctt
     3481 caattatgca aaatcgatta gggctttcgg tttctgggtc ttcattgtgg atggtcagta
     3541 ctattttagg catgtctttg gggatgatga cttttggcat aatcatggat aaattggggc
     3601 ctaaactagc cttttctttg tttttaattt gttcatcctt atctgtctat ctacttgtgt
     3661 tagcccatag ccagtggagt ttggtagcag ccgctgtgat agtcggctac tttatcaatg
     3721 gcatgtatgg tggatatggc gcagtgatta gtagtttgta tccaacagaa atccgcgcga
     3781 cggcgaataa tttcattatg aaccttggac gagctgttgg tggtttttct tctatcgtca
     3841 tcggtttctt gatggatcat tatagtttaa ctgccgtaat cttgtttttg agcggtattt
     3901 acctagtcag tctgctggtc ttgacaactc ttactggtgt tacagcatta agggaaagct
     3961 tgcagcattg atagttgagt ctgtaaatga caagaaagag gtgtggcaaa agcttgcatc
     4021 tgcggtaata agaccagcca aatcaaaaac atgagacact gtttccgttt ggctggtcgt
     4081 attacttgat gcagggcagc tttttcaact gctaatttcc ttctatctgg cggcgactat
     4141 tctttgacgt ccgtcatttc aaagtacttt actaataact tagcataacg aaaaactgtg
     4201 gatacactcg tatattgagc gtctgcataa tcttccaaat ttttctcagg atgttgatac
     4261 atgaattcaa tcaaatgata attatatgat tttctgtaga aatgaagata aataatgtag
     4321 attgggaagt tctcctgctt ttccatagaa aagcgccgat tattctcatt cttcaaaatg
     4381 accttatcag gatatttttg gaacaattct tctaaatctg ttagataatt caagatagac
     4441 ttacttgata ccttcaattc gttttgtagt tcttctattg aacaccaacg ttctgtttca
     4501 agcagtaaat gaatcatttt aattttcttt tgagaaactt ttgttaaaaa agtctgcata
     4561 ttttctcctc ctgtatctac tttactatac tacatacaag aaaaaaaacg gatacaaata
     4621 tcatattgtc acaccgtcaa tagtgcataa acctgttatt atttctcttt ttttcctaag
     4681 tattctattg atatgaatca tttaatcgtt tattatataa atgtcaataa tctaataata
     4741 aaataacgta catattttat tattgcatac ttaataacag aactatagca aaaaatcccg
     4801 tgtatcaaca cgggattaga ttaagtttcc tcaatcacta ttcataacac aattattata
     4861 acaagtaagt aaacaattga aaaggacatt tattgaaaac ggttgttttt tacttaagga
     4921 ggaaatttag cgaaaaaatt agttgcattg tttttggtcg caggaagttt gttgagtggg
     4981 tgtggtgcta ctacgtctac tactcaaagc acaagttcag atcaggcttc cagcagcagt
     5041 accacgatct ccgaaagtac ggcagattca tcgaaagaac aagaacaaat tgaaaagaaa
     5101 aaagaagaga aacaaaaagc ggaaaaagct gcactagaaa aaaagtgaca gaagcggaat
     5161 ctgctttgtc acaagctgaa gcaaatccta caaaagaaaa ctatgcaact gcttcagcag
     5221 ctatccaagc gctacctgaa aacaaacaag agttgacaag ccgtcttgct gcaattgatt
     5281 ccacaatcaa agcaaaagaa gcagaagaag aacggaaaaa gcaagaagaa attgcacgac
     5341 aacaagcaga acaagcgcaa caagaagcaa tcgcacagca acaagcagaa caggcgaggc
     5401 aagaagcaat cgcacagcaa caagcagaac aggcgcgaca gcaatcagca gctgaacagg
     5461 cctctcagca ggcacaagcc gcagctcctg agcaaaataa tgaacaaact gtctatgtca
     5521 caccgaccgg ttcaaaatac catacacata aatgtggaaa tggtacgtat acccctgcca
     5581 ctttaagcta agctcaagct cgtggtttaa ccccttgctc caaatgttat ggaaactaat
     5641 tcaaaagtta tactataata ataaaattct tctgaaacat aaaaaaatag aagatggcgt
     5701 tccagatagt aaaccaaaaa taacagttta cacgtccatt tcgtcagctt ctctcttttt
     5761 tcatttagtc tgttccctct gcttttttgg acatcttcat tcattctttc tcaatcaaat
     5821 agtacacctc tttttctttt cgcagtttat atcttccttt tttcaatttc attgaaaact
     5881 gtttcgaatg tttatgtttt acatggattt taagcaggag ctcctcatgt tgcaagcgat
     5941 agttcctgcc ttttaaatca gaaaatctca ctagtccgta ttcatcagaa gctttcacaa
     6001 atggaacttt ttctctagta atatagtgtt tgccgttgag cgtttttaca gaaaagacca
     6061 ttccttcgat tggtcgtccg tttgtgttct ttacataaaa agataaagga tagtttcgct
     6121 tagcgatggc caaagaatct aaataagcga tgagtagcca aactaagccg attgatccaa
     6181 gtagtatacc gataaaccaa ataaaaaatt tccatttttt ccattgatca attccttgga
     6241 tcgctttttt tgcctctttt gcttgataag gaatacgatg ccccgtcaca agcagtcgat
     6301 gtgtattgac catataagga gtacaggtaa gcaaagtcac gagatctctg cctttttgga
     6361 tacccaaaga atctacttca tcaggtaaga ccacttggat tttttctact tgatacgcca
     6421 atgtctttcc attgatctgg atgaaaaact ggtctccttt tttcaactta ggcaaatctg
     6481 taaataattt tgcttctggg agtccacgat gcccagaaag aacggcatgg gtgtttttcc
     6541 cgccagtcgg ataagaactt tcttccaata atgacgttcc tttttgcaaa aatatttccg
     6601 ttgtctgatc gaatataggt aagctgacat tgatttttgg gatttcgatg actccaagca
     6661 tatgttccat aaaaaaggca ttactaggta aatcttttaa tgcttttgca tcaactgctt
     6721 ggttgaaact gcctatccct ggtgatacgt tttgctctgc caactgttgg tttttctttg
     6781 tcattttttc ctgctgtttt tttatttcct ctgaattctt attcgatgct tgcttttgat
     6841 aatggatgat catttgctgg gcaagaaaat cattcagtga atcttctaca aaaggataag
     6901 aaaagatccc gatcccaacg actaaaatga ttgccatgaa gatgtccagt attcttattc
     6961 tgcttttatt catcatgtcc acctattttc taatatgtat atgaagaggt tgtgaaatca
     7021 gcgccttgac ctgagtatta gcgaagcaaa cggcaaaata gttcctccta ttttgccgtt
     7081 tgttgagcga ataccaagag accgaccact tgtcacaacc tttctcccca tttgaatcac
     7141 tatcgtttat ttatcgtatg atttttgttt ccaatagttt aagcttctgt ttcttgatgg
     7201 cgttttttga aatagatcac tgctcctcca actaaagcag tacctaccaa gatgaatacg
     7261 atgatcccca taccaccagt tgaaggcaag attcctttag gttggttatc gatcgttgtt
     7321 tcatcagaag ttgttggatc atcatctact gtaaattcag tgtacgcagc ttcattctta
     7381 ggtaatgcgt agccttctgg tgcttttgtt tcttgaaggc agtatgcccc ttgtttcagc
     7441 ccttcgattg aaaatttccc ttctgaatca gaaatcaatt tcacttcttc ccagtcagaa
     7501 cctgtgactt catctgacca agcatatggg tattctcctg aggcaaggtt tgctattttt
     7561 ttcattaatt taacagttgg atcttttgct cttagaatag aaaattctgc atcggctaat
     7621 ttttcttgcg tgttcacatc ttgtttgatg aagtggaaat caccgtagtt ttggacattt
     7681 tctacagtaa tcgtgttatc cgtagttgta ttttcccgat tgactacacc gtttggattc
     7741 ccaagattgg cttcatctgc gccatggatg ataccggttg tttgttgcaa ttttccgttt
     7801 tttactataa aagtagtttc tttttgtgga ttggttaatt gataaccatc tggtgctttt
     7861 gtttcaacta aagtataatt gccgtctatt aatccattga cactgatgga tccttcagta
     7921 tcagttgtga atgtcaacgc tgactcagca ggatcataga gagcgacacc agtgttttca
     7981 tcaaaaccgg tcacgaattg attctctgaa tttttgatct ggaattgcgc ccctgctaac
     8041 ttatctaccc ctgaaaagtt cccagctttc tggattttga tattgctttc tttgatgaca
     8101 tttttagcat ataggtgaat cgtgtctaaa ggtttgtcag ggtctagggt aagcttaaca
     8161 cgatatttgg cgacgtttcg acaccgggct ttggtgtttc tacaaagacg tatacagcat
     8221 ctttcccatc agatttacct ggaagagtca ggttagctaa tccttcacca gaagtcgtta
     8281 cacttgcgaa gggtgtagcg tctgctggaa ctgctgtgtc tttacttgga tagtacgtag
     8341 cagtgatgtc atatacgttg aattcaacgc cgttcaacgg ttcgctattc ggaaatgtca
     8401 attccgttcc gttgtttatt tgattttctg gtgctgaagt gaatttcaat ttatgcaaat
     8461 taaccgtaac attttctggt gtaggtgata cggctgcaga aacctctgca gttcctaggc
     8521 cgagaacgaa aggaagcagc atgatagcag ttcctaaaaa cgtccatatt tttgttttga
     8581 tgtttttcat tttatttccc tcctattgtt tcctttttgt tgttttttat aaaaccaagc
     8641 tgtccctgtt gaaaggacta gcaataaccc taaaattgag aaacctccaa gcatctcttc
     8701 gcttgttttt ggaaagcttc ctccattttt ttcatttgat ggaccatttg tctgcatctg
     8761 aactggcgtt tttccattat tcgtcggatt tgcttttggt tcgttgatga tttccaatgg
     8821 tataggatga agcggatcat aactgccttt ttcaacttca aagagaatgg ttgattgact
     8881 caatatatat cctttaggcg gtttaatctc cactagttcg taacttccaa aagacaatcc
     8941 atggacagaa aatgttccat ctttactgga agtaaaaatc gttgctcttg aatcatcttt
     9001 ggctacccaa tgatttatcc cgttttcttg cgctaagtat tggtcatttt ttttgactag
     9061 aaattgagca tctgctagtt tttgtttcgt ttgccgatcc actttgacaa attgtttgcc
     9121 tcctgtttgg atggcaagat gtgtttctac tgtttgattg tcagtcgtca aacgtacatc
     9181 gttatcaaat gtcgtttttt cactgttttc tttcaatgtc atttggtaac ggatcgttaa
     9241 tttttttcca gcaaacgaac taagatcttt tccagaatct gataagacaa aaccatgttt
     9301 tattttttgt gtgtgaaatg tatgaatctt gtctccacca atcaaaaaat cgatcgtttc
     9361 tggttctagc cataactgag gatcagctgt atctttgatc gtataggttc ctatctcgtt
     9421 gatgttctca ggaattgtta cggtgatgag ataagggact ttatccccat ctgcaaaatc
     9481 tgtctgctta ttctctactg tttttgttaa tgtgagtgca gcaggtattt tttgttcgct
     9541 ctttggatac aagtggatgg tcgtcaattt atgattagat gaatctgtca caggaagcac
     9601 aacgacaaat ggtgctgatt tttcttttac ttaatctgaa gtctttattt ccgtaaatcg
     9661 gtaaacagcg tcacgacctt gctcatcttt gtccgatgca ctaaaagaag caatgccctc
     9721 ttcctcgtct actgtttttg tcacattttc tgccagtatt ttttcactat cacttttttg
     9781 tgctagtttt ctttgtgcgt cttccacact acttccttct gaccgcagct tgtaaaattc
     9841 atccgagaca tcatatatag cgaaagtaac gccgttcaac ccttttactc cttgaaagtt
     9901 atccgggata tttattccgt cgttttgact gatacttggc atcttatcgc tttcgaacat
     9961 tcgattatgc aaagtaaatg taataggtga ttctctagct gacacagtcg gagaacctat
    10021 aaaaaagacc agtaggataa aaaagagaac agcaaatata tccacacgta cattttttgt
    10081 ttttttcatc gctgttcacc tgcttttttc ttacgatgga taaaataaaa gaagccagaa
    10141 aaaataaaga tcagtcctaa aaagacaaag ggcagtagac ctatcccacc tgtcgaagga
    10201 agcaagagag tcggattgat cgtcaactta gcggtatctg ttgcgataat cgtataatta
    10261 ttgtttacac ttccttcgcc taaactaatc gttactattt gtgatccaat atctgtccgg
    10321 ctgttaactg ttagggtata atccaatgat tcgcctaaga caacaccggt aacgtggaga
    10381 tttccaaccg aaatcgagtg actttgggca tcataattaa aggcagcgtc atcgacagta
    10441 atcactgcgt tagctttata gatggtataa gttcctgatg tgttggctat atcagtaaat
    10501 gacaacttgg gatttgctgc tttgactttg tctattccgc tattcgttag tttaattgta
    10561 tagctgccag cattaaccat attttttgct gattgtgcat ctgctgtgta atccatactg
    10621 ctaagtgcca cttgtactgg agtaagcgaa ctagtgacac cctctgctag cttgacatgg
    10681 attgacggga cacctgcttt tccatcatat gtcttactgc cactgagctc taccgcaccg
    10741 ctgatttgct taatcgtata gacatcagat gtctgcacga tagtaaataa atagtttgtt
    10801 ctggcagcta gtttagctag tccgcttttt gtcaattgcc aagacgtata ggttccaatt
    10861 tcagtaggcg cagttacttc aactcccttt tcattcatcg ctttgaaatc agttggattc
    10921 catgtgattt gatcaatact gagatcattg tttaaaaggg atgttgattc tgttttatca
    10981 ttaaaggcat atttaacgat cggtacaaca gaaagaccat catagtactt actgccattt
    11041 gcattgattg atactgttgc atttgtctta taatatagga tgacttcaga aatctgtttt
    11101 ccatttgcgt cctttacgat tacatttcca tttgtatcta aactagctaa aacactcatt
    11161 gctgattggt tctttgtttc tatcgctgaa acagctgtaa aggtatatcc gttgatagta
    11221 ggcgtattag tcgtcaggtc ttgtgcagca cttccgtaag taagattcat tattttagaa
    11281 ggagcaatca tctgtccaaa atagtcttga tacttaatag tgattggtgt ttgtttgcct
    11341 gtataggtca ccgtatcgac gttactttct agggctgtga aagaaccgtt tgtttgtgca
    11401 aaagaatagc ccgttactgc tgttggagtg ataaccactt tgtcacccac atggattcca
    11461 gataatgtag tcccgtacgt tccgattttt gctgctccac cattgactgt ataagaaaaa
    11521 gttcctgtcc gattcgcatt gacctgaacc gttgctgctt gataggttgg aaaatttgaa
    11581 acatttccct taccatcaac gatctgccca tttttgatcg agtaccccgt gaatggaata
    11641 tcatttgcta actgtatacc gattgaaaaa gtactcatac ctcctccggc aggaagccct
    11701 ccccatgttg agggtgtaaa agaataactt ttatccgcat tgggtgtaac tgaatcgatc
    11761 gtatttccag cggtagccac ccccatatag atactttttg ggaatgtgat agtagtacca
    11821 tttactgtgg agccaataaa cagattatgg atatctggac ggttatcaat aaaagcgatt
    11881 gctttggtta cgccttgtaa cgcagtttta tccgcactca tcgatccgtt aaccgcaaca
    11941 atgactccat ctttggcata tatcactgta tatccatctg gtatggtatg tccgttgaaa
    12001 ttcgcagtgg ccgcaacagt actaaatgtc tggcttctac ctgtttctga ataagctttc
    12061 tcccctgctg cttttgctgc tgtgatttct ttttctgtcg gatcaatcag ttgagtcgag
    12121 gatgtggttg aggttgtctc gttgttgcta gactgattgg tgctttctat ggactgtatt
    12181 tcagaatagg gtggactatt tgtctcctct gtcgtttggt tagcaaaatt tgacgtttct
    12241 gtataaacgt ttacataaat acttgataag ctccctaaga aagtaaaaat caagatgatc
    12301 gaaatcataa tgggatgtaa gtattttctt ttagatacag ctttgagtct agaaacaaaa
    12361 tgtttttgac atatacgttg gattttattt tgagttttct taatcatttt catccctcct
    12421 ctcaataaa
//
LOCUS       DBNICI010000085        12265 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.085, whole genome shotgun sequence.
ACCESSION   DBNICI010000085 DBNICI010000000
VERSION     DBNICI010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.085"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(44..1693)
                     /locus_tag="AC1ZCC_001828"
     CDS             complement(44..1693)
                     /locus_tag="AC1ZCC_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MutS-related protein"
                     /protein_id="HIQ1630074.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(1810..4413)
                     /locus_tag="AC1ZCC_001829"
     CDS             complement(1810..4413)
                     /locus_tag="AC1ZCC_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HIQ1630075.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(4425..5474)
                     /locus_tag="AC1ZCC_001830"
     CDS             complement(4425..5474)
                     /locus_tag="AC1ZCC_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HIQ1630076.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(5500..5814)
                     /locus_tag="AC1ZCC_001831"
     CDS             complement(5500..5814)
                     /locus_tag="AC1ZCC_001831"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HIQ1630077.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(5830..6276)
                     /locus_tag="AC1ZCC_001832"
     CDS             complement(5830..6276)
                     /locus_tag="AC1ZCC_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIQ1630078.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(6277..8199)
                     /locus_tag="AC1ZCC_001833"
     CDS             complement(6277..8199)
                     /locus_tag="AC1ZCC_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HIQ1630079.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(8411..9307)
                     /locus_tag="AC1ZCC_001834"
     CDS             complement(8411..9307)
                     /locus_tag="AC1ZCC_001834"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIQ1630080.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(9300..9980)
                     /locus_tag="AC1ZCC_001835"
     CDS             complement(9300..9980)
                     /locus_tag="AC1ZCC_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIQ1630081.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(9981..10763)
                     /locus_tag="AC1ZCC_001836"
     CDS             complement(9981..10763)
                     /locus_tag="AC1ZCC_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIQ1630082.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(10756..11673)
                     /locus_tag="AC1ZCC_001837"
     CDS             complement(10756..11673)
                     /locus_tag="AC1ZCC_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIQ1630083.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(11837..12091)
                     /locus_tag="AC1ZCC_001838"
     CDS             complement(11837..12091)
                     /locus_tag="AC1ZCC_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630084.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
BASE COUNT         3616 a         2536 c         1924 g         4189 t
ORIGIN      
        1 aagccaagat ttttgatcca ataaatactt tcgatcactt cgcctatccc aatacatgcc
       61 actgccgctt ttcatcaaat aacgcggctt ccgtttttgc ggtttctact aaagtctctg
      121 ggtaatccaa aactttcagc aaggcaatcg catttcgggt ccttgaaggt ccttctttta
      181 atttgaaatc aaaactgatt ccttttcctt ctgttacttg ttcttcaaag tggacattct
      241 cacaataatt ctttaaaatc tctgtcaatt cgatatcatg tgtggcgaca aatgctaaag
      301 atggatactc cgcaagccac cgaacgactg aagcggaaga agcaatccgt tcgatcgtat
      361 ttgtcccttt taaaatctca tctacgaagc agtagcagcg ttctttggaa gccacttggt
      421 ccaacaaacg tttgatcgat ttgatttctg aaacaaaata actgtcgcct tcaaataaat
      481 catcttcgat tgccatggaa gtcaacacat gtccaggttg cagggtaaac tgttctgcaa
      541 tcgctgtttg gatcgtctgt gctagaatac agctgatggc tatacttttg acatacgtag
      601 attttcctga tgcattggaa cccgtgacta atgtattctt ctgccagttg accggattca
      661 ctaccgcttc ttttaacaat ggatgataga tatctttcgc tgtcacaccg ccctcagtaa
      721 actctggtcg gcaagtgatg ggcatataag tacggtaatt caatattgca gcggctactt
      781 ctaattctcc cagcaattcc cacagcttga tggcttcagt tgaatgcttt tctaatttag
      841 tgagaacgaa atgataggag ataaatggca gcatgaacat gatgttgaca tattcaaaaa
      901 tcaactctgc ttcactccca ctcttcatac gaaatgaaaa acctaattta gggatgtctc
      961 ttaatggttt agtttgctgt ttcagctctt cttgaagtgg tgtcggcagt tttgctagct
     1021 gattggcggc tgaaatcgtt gaaaccaaat aactcatgct attcagctct gtttctagtg
     1081 tttgtttctt cactagataa tagaccgtat tgaatactaa cccaccgatt gccaaataga
     1141 ttcctagtac ttgtcctaaa agaagcaaac ccaaacccac cagcggaagc aagccaagaa
     1201 aaataaataa acctacgcgt ccaatctttt tactttttcc atccgctaga tattgttttg
     1261 caaaattatg atcttttttt cctaaacgag cgaattgata ttggattttt tctctcagtt
     1321 gaggattttc ctgataaaaa gcaatcagtt tttctaactg ctgatcttca ccaaaatcaa
     1381 agctgcgtaa acgttgatac aacgcttctg aacctacact agaataggta ctgttgattc
     1441 cttcgaatac ttcgaacata tccaggtcat accaagtaag atcatcgatt tcgctatccc
     1501 atgaacggaa cttcttctcc gtgagccatg cttccttcaa gctttcttct ttatcaaatc
     1561 tcggttgata aggaattttt tcccatttgc tacgaactgt ttcctttaat ttcaaacgtg
     1621 aaaaaatgtc taaagcaact aataaaataa cagctgcaat aataattaaa acgataaatt
     1681 ctctttgacc catatccctc tccgttcctt tatttgttag ctttagtata gcactgttgc
     1741 gctatttgaa aagagggtaa aaaaacacct ttcaaaataa aaggtgtttt tttcttgtat
     1801 attacatatt tatctctcat ttatcaaaat tgacaaataa tcatagggag gaattgttgt
     1861 taattcttgt ttattctcca gtgcattgac aactgtccca agggaaagga tagcctcagg
     1921 tactattttt tcttgttctg ttggattagc taagcgaaga acaaatcctt gttcatgaaa
     1981 ataagaagga taaatcgatg atacaattaa gtcttcaggt aattcaatca atgaaaattt
     2041 acgaggaaca tcaagttcat tttgtaatgg ccaaatcttg ttatctaacc tgtgaataaa
     2101 tacattcagt ttctgctttt gataagaaat agattgttct aaccgttttt ctgcctgttt
     2161 tgccactttg aactcattaa atcttccttc ttctacatta aatccaaaag tgactttata
     2221 ctcacctatc tcttgagcta gcggtgtttc catcatgaca tgcccttcat ttgtcgtatc
     2281 tcctgaagct cgcccaggtc gccaagcaag attaggtttt ccaagttccc ctgttgttga
     2341 aaataaggtt atgattaatt gattttctat acgttcatac tcttttaaac catctgcata
     2401 aaacgtaaaa taatgatcat tttctgccac agagacactc ttatcgaata tttctaaatt
     2461 aacaggtttc tctaccatag tttctgacca attcggagga atgacatcag gggtattttc
     2521 aataaatccg ttttgaattt gtgcaattga tttttcagat ttatctaaaa cattaattgc
     2581 taagcgcata cgatgcgagt aaacagtatt atgaattttg agtgtgcctt caacgatttg
     2641 actttctccc agactgaacg atatttcata agatatttct tccatttcgg gtttttcaga
     2701 taaacgatct tttaaatctt taggaagttg agcttttccg taaacaacta atgtttcttg
     2761 taaagaatct ttatatactt tagctgtttc taattttaga gatagttctg tatctccttc
     2821 taatggtgaa taatcatacg tatcgccatc atttgctgaa tcgatcaaat gaacgaaatc
     2881 aagatattgt ctatctccaa ctatcaaatt caattgccca tctttaaaag aaagtttata
     2941 tttattgtta cttatctcag ttttgtctat ctcgattgct gtttctaatt tttcattatt
     3001 tttctcaaat tcaacaacag tgtatcctag tgctggcaca tttacattta tttttatgtc
     3061 aagttcataa taagctggtt catcaaaata ttcgaaacct gtagcaatca tccgataagc
     3121 atttttccgt tctggaaaat aaacttcttt ttcaatgata ttatagggac tacctttgaa
     3181 gtgtattttc tttgaatcag ctactacatg cacgattttc gatccactat aattctcagt
     3241 cgttgtgttg aaaacaagga cttcattcga ttttagcttt aatgcatcag ccatccgttt
     3301 tacaataagg ttttcgattc cgtcagctat ctcattagct tctttgattc gatggaaaat
     3361 atcttccgca acattatctg aaacacagcc accaatacta tcatgagcct ggttttgcaa
     3421 aatctttttc cataaccgac gaagtaggcc tttactgata tggataccat ttctttctgc
     3481 aatcagcatg agcggctcaa ctcgttttag aattttttgt tccaatttaa aattttctat
     3541 tttcattttt gttcttactg agccaataga acgatgaaca cgagagtaaa ccggctgacg
     3601 aagttcacct ttatattcag gtaattcttt tttgtttctg ataacttcta cataagaagg
     3661 ataatctgaa ataatattgt ggaatttact gtatttattc aacttctcta cgacttgatc
     3721 aaaatttaaa atcattgact tttgatctac acccgaaggt actaaaatat ctttgttttt
     3781 tccttgcata tgaagcattt caactgcttc atccaacttt tcttttgcaa attcaggaat
     3841 atctttaatt gcgtctaagg tcatcattcc agtcatataa ccaaatggaa aattcatggc
     3901 ataaacaaat ttatctccca atcctttcca gataaaataa ggagatggaa ccatcgtttc
     3961 aaagtttaat cctcgccaac acatgaaatt atcgatttct acttgatgca aaagtgtagg
     4021 caaattcgca ttgaacccaa acgtgtcagg caaataccct accatcattg gatctccata
     4081 tttaaggatc gtatcactga ttccaatcat taagtttctt aaaattgatt cagcatccac
     4141 taataaggca tctgtttgtg cataccacgg tcccacgaat agttgtcctg ttttcatcag
     4201 tcgttggata accttaagct tttgcggatt gatctccaaa tactcatcga cgattgaagt
     4261 ttgtccatct aaacaaaagt ttactttagg gttcctttcc aattcatcta gaacttcagt
     4321 aaagacctga tcagacaata cgatggcatc ttcatttgta aaataccatt ctctgtccca
     4381 gtgcgtgtgt tgaattatat gtgcatttac catatcagat tctcctactt tccttcttct
     4441 actgaattta cttgttgttc ttttttatac ttgatggatt ttagtgcatt gaccattaac
     4501 gctgtaacca ctcccccaat aacaattgat actaaatata aaggtatatt cgtaacaaaa
     4561 ggaattgcca taaaggttga aagcattgtt tcttgagtaa caccaaaaaa tccatttaga
     4621 ccacatgcta ctgcagtgcc caccacgatg cttggtatta ctcgtaaagg atctttcacg
     4681 gcaaacggga tcgtcccttc agtaatcccc acaaggccca taatcatagc tgtctttgca
     4741 ttatctcgtt ccgattcatc ataaagtttt ttgtttaaaa ttgttgctaa aaacattcca
     4801 aatggcggaa tagcaattcc taataacatg gctgtactag gtccaaagct tccgcttgaa
     4861 aaagaagcaa ccccaaaagc atatgcgatt ttattgatag gtcctcccat atcaaacgcc
     4921 atcattcctc ctaaaatagc cgacatcaaa gcagcatttg ctcctgacat gccttctagc
     4981 caattagtca gtcctgttgt cattgcttga ataggcgtag ctagtacata ccataatatg
     5041 attccaacaa ctaatgtagt aacaaaagga atcagcataa ttgattttag actaataact
     5101 tgcatcggta cttttacttt tttcaatgca ttagctaaat agccacaagc taatccagca
     5161 accattcctc caataaatcc cataccaaga ttttgacaca tcatcccagt gataaaagct
     5221 ggtgctagac cgggacggtc agcaattgaa tatgcgatat aggctgaaat catcggtacg
     5281 ataaactgta atcccgtctg accaatcatt cgcaggactc cccagaatgt atccgtctgg
     5341 tcccaaacac ctgtgccacc aaaaatgact gcaatagaag ttagtaaccc tgctgctacg
     5401 acaagcggga tcatatagga aaccccactt tggaaatgat ttagaatttc tcttccttgt
     5461 ttcttgaatt ccattagtaa ctcccccacg atatattttt tatttgttaa ttaatgcaat
     5521 tgcttcatca atcgtctctt caccatcttt tactacctct gaggtcccta ctgttaaaat
     5581 cggtttaccg tcaaagcgac ctcgttctga aacatttgta tctactgcaa atatgacaac
     5641 atctgcctcg gaaacatccg aaattgaaag tttgttttct atccccatag ccccctgtgt
     5701 ttctacttta atatctaacc ctttcttctt cgcaaacttt tctaaattag cttttgccat
     5761 ataagtatgg gcaatccctg cgatacatgc tgttactgct actatttttg tcatatgaaa
     5821 ttcctccttt taaaatgata gagcttttac caattctttt ttatttctcg aatcttttat
     5881 tcttttaaca aactcgtcat ccatcaatct tccagataat tctgctaaaa tacgtaaatg
     5941 attagtacct ttatcagtgt cttttattgc taacaaaaag acgagatcaa ctggttgatc
     6001 atctaatgcg ccccattcaa cagaccgctt taacttggct acagccactg ttgattcaat
     6061 tactgctagg cttttgccat gaggaatggc gatatttttc ccaattcctg tggtcatatg
     6121 gttttctctg tcaaatacgt ctttttgaaa ttgtttgtgg tcagacacaa acccattagt
     6181 ctctaacaat ttcgataatt tttcgactac acttttttta tcagatgcat ttaaatctat
     6241 cactatatta tttatattga ttttttcact gatcatttat aagccttctt tcaatatata
     6301 aactaatttt tcaaacgacg aactcgtttt taaatttgct aataactcat gattcgaagc
     6361 gatttcattg aatgtttcat aaattttatt caattcaggt tttacttttt cattaagtgc
     6421 aaaaaagaaa acagaatgaa caagtgtccc atcccaatca attccttctg gattaacgta
     6481 gacacagatt actggctttt ttataaaatc tacttcgata tgcggcatag caaaatcatt
     6541 catcgctgtt gaagatagtt tttctctttt gattgctccc tctagtatcc cttctttcgc
     6601 gtaattttct tttaccaatt ttttggtaat tatatttaaa acgttcactt gattatcctt
     6661 tatttggtgg gtaaaaaagc aaaatcgttt atctaataaa tttagaaaag catcttctgt
     6721 ttgttcattt aactgtagat actgttttac ttttctgaca tctgccccta acatcaaagg
     6781 actaacaaca ataacaggaa aatttgtatt ttcaatcgga atcgtgctta taacaagttc
     6841 atcagtgact tttctatctt gtagttcatt gatgcttagt acatcttcta tctcaaccat
     6901 atctgcaaaa ccgttattta ttctttgctc taataatttt gatgttccat atccgctact
     6961 acaaactaat atcactttcg ttttgtcagg tttgtaacga tccaaaagtg attgaatatg
     7021 gagtgttacg taagcgattt catcttcatt aaaatgtata cagaatctct cttcgatttc
     7081 ctctaataaa tctacactta tcataaatga ccttttaaaa gaatatttga ttttgtccgt
     7141 ataagggtta tagatattta ctcccaattt gagtctttta actgctgcat tgagatgtaa
     7201 tactagatta ttgatcaatt cttcatcagg ataaaattca tttaatttaa ttcttactaa
     7261 ctcttgtagc tcaacaccca tatctaaata ggaaagcttc gtttgatcta agtcgtgatt
     7321 catagcattt ttttcaatcg aagtaatgtg aatgtcaaca taagcttgtt catctattgg
     7381 cacaataatt gaaaatgctt tttcaatcat tttgattaaa ataagtgtat tctttgagat
     7441 ttcttcttca gaattattac tacgtgataa gaaattgctt gtttcaatcc tttctatcgc
     7501 aatagcaagg tggatagcta aagtttgaaa ttcataatct gtaaatttca attgtgtctt
     7561 attcatgtat cgattaagca attcggctac ttttttcaat atgtccttat ttaagaaatt
     7621 attcgcttca ccaatgaaat tgatattcaa gtctaagaga tctgttttac tgttagatgc
     7681 tgtaaagcca ccccaataat agttcacaac ttctgacatc aaccttcttt tacttttttc
     7741 atcagctttg atgcgcacgc catttcgatc tgaaactaag gacatcccat gcttgtttag
     7801 tctttttcta acatctttca ttgtgctttc taccgtcgac ctgctcacgt ataattcgtc
     7861 agcaagattt tgaatcttta gagaatcatt agtattcaat aatttagcaa acacataagt
     7921 tagacggtcc tccggagagt ctgcgatatt gattttatga ctggataata aatttcgaag
     7981 tgtttctttt tttccaataa gcgaaacgcc ttttttaggt tgaaccaaca tttgtacatc
     8041 tggcattttt tggattattt ctttcaaaat taaaatatct ttgctaattg ttttcgtaga
     8101 tacatataat tgttctgata attctttgta agttgtgtgc tcattttcaa tcaaaaagta
     8161 tagtattttc atttgccgtt cgctaaattc gatgcccatt atcttcacct cctgaataca
     8221 gcttaagtgt acaattatta tctctatttt catacagaag aaagttcttg attaatgtag
     8281 aagtttctaa tattcttgat tatttcaaat caataaaaaa gcctgcaata tttatatcac
     8341 aggctttttt ctctattaaa tatatataag ataatacatt tttctactag ttctcactaa
     8401 tcttgtataa ttaaaccctc cgcaacttga cggagatgga gaagatgccc ccgtctaatc
     8461 tagcggtcat ctctcctccc attccttcca ccaatttttt tgacaggtaa agcccgattc
     8521 cgctagattc cgtatttgtg cggctggtat tttcggtata aaaacgctgg aagacttttt
     8581 caacagaggt ttgcgttcct ggtttgatag gattggccac tgttaatcga atgaatggat
     8641 cctcttttga aaatgtcacc tttgcttgtc catctgcata acgcaataaa ttctgtatga
     8701 tattttgcag cactcgcagc aacatgagcg gatctgctaa tacagttgta tttatcgagt
     8761 gttcagcgaa cgctacctcg atatttttct cagaaaattg ttcataaaaa gagaaaaata
     8821 cttcttccac ctctttagaa aaagaaagcg tatggagatc catttctttt tgcttcgtct
     8881 ctattcgagc aagatcataa aactgatccg tcatactgag taatcttgca acagattgtt
     8941 tgatgatcgt taaatacttc tcccgtttct ccgtatcctc tacctgttcc agtaattgta
     9001 cataaccgtt aatagcagtc aaaggagttc tgagatcatg agaaaggccg acgatcgata
     9061 gtctgatgtt ttcttccatt tcttgaaatg cttgtccttt ttgttcattt tcatccaacc
     9121 atgtattcag tgcatctact aggtccatta gattcttttc tcgaaattcg actgaaagac
     9181 gaccaccata ttcttgtcgt tcagaaagag tggcgatttt cttagttaat ctcttgattt
     9241 ccctttgcag gaagaaaata aaacttccca taaagataac aacgagaagc aacagccatt
     9301 taatcaaatc taaaccccac tccccacacc gtatgaatat acacagcatg tgcctttgct
     9361 agcttccctc gaagattgga aatatgcata ttcactgttt tctcactatc aaaatatggt
     9421 tcttgccaaa ctgtttcata aagatttgct cgcgaaaata cttttttcgg atgttctaaa
     9481 aaaagcttca gcagatcgaa ttctcttcct gtcaaatgga cagccacacc attcacattt
     9541 acatccctcg actctttgtc cagtaagatt tccttgtaat agatgcgttg cgacagatct
     9601 tttacttgga cagaagtctg atgacgaagt tggatgccga ttcgtaacaa aagttcttcg
     9661 atatcaaatg gtttcgtcac atagtcatcc gctcctgctg ccaacaagga aactttatct
     9721 ctttgatcgc tttttgctga aagaacgatg atcggaacat tcgatgtctg tcggatttct
     9781 gctaacaatg cttcacctga aagccctgga agcatgagat ctagaaggat caaatcgaca
     9841 ggatatgtgc ttaacaataa ttttccttcc gtacctgaat aagcatcaag aagtttgtat
     9901 ctctgttcaa gaacctcctc cagtaaggaa tgaacagctt catcgtcttc gataatcaaa
     9961 atagtttcca gcaggaccac ctacttaatg tcagattttt ggaaaataaa cattcctaga
    10021 gctgatgata ccacgattgt tactagactg accagaatca ttttcggtgc ttgcgaataa
    10081 gttgcttgtt gcgcaaagtc cgtatacatg agccacgttt ccgtaaacca ctccgttaga
    10141 aattccaagc ctttgatttt tgcgaaaaca ccgggaatga aggccgttcc catgatataa
    10201 agaaaagaaa gaagcaaact gacactggta cttctagtga tgaatgtcaa agccactgga
    10261 acagataatg tccctaatgt cagcagaagc aatacaggaa atacttttag aacataattt
    10321 ccatagtttt tgattccctc aacttcacca aatccgaaag caagtgtgta agcaagcatg
    10381 gctagtcccg tgatcaaaac cacgccaagt gttgtgatca aaagcgtaac gagaagtttc
    10441 gacacataat aagtcattcg attccgtcct aaagataacg tattgcgaat cgtaccgttt
    10501 tgaaattctt ccccaatata aaagcacgca aatgctgcga aagccagaag aatccatgca
    10561 gccatttttt ctaccattaa aatccctgtt gcaccgttga tgactgtatc cgttgcttgg
    10621 tttaattcat tgccgtattt tgtatgcata taccattcgt tccaagaata aaaaagcgga
    10681 agtacgacca tcagtcctag tgagacccac attttacggc tcttccatac ttttattttt
    10741 tcagcagtta atagattaaa catgtggatt gcctccagtt aattgtaaga aataatcttc
    10801 cagtttttgc ccaactaggt gaataccatc cacaggaact tttgcttcta ccaatgcttg
    10861 attgatggct gctacttggt caattccttc aaaaagctgg ataacacggc tagattgtac
    10921 aaaataatcc ctataaccaa gagagtcgag aaccgttact gcttgttccg tcgccgaagt
    10981 gtcaattttt ataaattgac gagattcttg tgccaattct tctttcgata attctttgat
    11041 caagtgtcct tcatgcaaaa taccatagtg agtggcgatc tgtgacaatt catctagcaa
    11101 gtggctagaa agcaagacgg tgatgccccg ttcttttacc aaacgttgga tgatttctcg
    11161 catttctgcc atccctgctg gatccatccc attcgtcggt tcatccaaaa tcaaaaattc
    11221 tggattcgtg atcagtgcat ttgcaatagc taaacgctgg cgcataccta aagagaaatt
    11281 ctttgctttc tttttccctg tattttccaa tcgcatcatt ttcagcaggt cttcgatttc
    11341 tctatccgac acaccgccgt tcgctgcttg gatacgtagg ttatctcttg cagttaattc
    11401 tggataaaga gcaggggttt caatcgattg acccatacgt cgtcttgctg cctgcaaatc
    11461 cgtcgttcca aataattcga tcgaaccttc atcaatcgag atcaaaccca taatagttct
    11521 cataaaggta gacttacccg ctccattttc cccaatcaat ccatagatca ttccacgttt
    11581 gatttcgatt gatactttat ctaatgcttt ggcagcaccg tattttttgg ttataccagt
    11641 tgctttcaag accgtttcat ttccaaaatc catacttatc cttctttctt attgatgagt
    11701 gaagtatagt tgacgggtat aaagaaagca gccagaaatg tataaagaaa gtgtaaattt
    11761 tccaattgaa tgtcaaaaaa aacaatcgga caatacgtgt ctttcctatt atcaggcagt
    11821 ttgttatacg cttgtatcag tttcctgatt ctttcttttc gaccaaagtc gtttctctgt
    11881 attcctcttc actgaaaatt tttgcttctt tcagatcaag cttgagtaat tttaccagac
    11941 cggttataag gataactata gcaagactcc ctcccagaaa aattagactg tctcgcagac
    12001 gtggttgcgt tgtcttccaa tcaaagagcg caccaaacag tgctaatcca agtggactgg
    12061 ccaccatggt aatcgttgtt atcgttgaca taagtcgtcc ttgtatatgc gttggaactt
    12121 ctcgctgaag ccagaccata taaaaagcaa aagaaaacat taatccttaa aaacggactt
    12181 atgaaaaata atacatgccg tttgggattt acaattagct accaaataag aggttatgac
    12241 aaaagacttt tcacaatccc ttctt
//
LOCUS       DBNICI010000086        12218 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.086, whole genome shotgun sequence.
ACCESSION   DBNICI010000086 DBNICI010000000
VERSION     DBNICI010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.086"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..1287
                     /locus_tag="AC1ZCC_001839"
     CDS             <1..1287
                     /locus_tag="AC1ZCC_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HIQ1630085.1"
                     /translation="ILGLLAGMKLKKAFMSALTLGIAFSGMSMVIGYMSNAVSPASEA
                     LAKNTGISLPALDLGWTGAASITWSWSYAFIFFAVTIGVNFVLLLFNWTKTLNVDMWN
                     VWGKALTAYLVYYVSGSLAAGFLTAMVQVILELKLGDMFQKHIQDLTGIPLVTVTHFM
                     TSAAVLLLPFNMIMDKIPALNKRADTNALKKKIGIFSENSVMGFIIGLGLGFAAAYGV
                     SGSLNLAIQTATALSLFPMISKMFMQSLSPLADAMSEFMKKRFKDREVYIGLDWPILA
                     GRSEIWVTAILLVPVFIGYAIILPGNQVLPLAGIINYSIAVGGLLLTGGNLARMLILG
                     IITMPIYLYGATYLAPILSDLAEKSGAVEGIKKGQLITWSSIEGPEFRVLFAEAFNRN
                     ILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAELQK"
     gene            1309..1569
                     /locus_tag="AC1ZCC_001840"
     CDS             1309..1569
                     /locus_tag="AC1ZCC_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630086.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            1583..2335
                     /locus_tag="AC1ZCC_001841"
     CDS             1583..2335
                     /locus_tag="AC1ZCC_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIQ1630087.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2429..3034)
                     /locus_tag="AC1ZCC_001842"
     CDS             complement(2429..3034)
                     /locus_tag="AC1ZCC_001842"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIQ1630088.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3087..4061)
                     /locus_tag="AC1ZCC_001843"
     CDS             complement(3087..4061)
                     /locus_tag="AC1ZCC_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HIQ1630089.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4439..5158)
                     /locus_tag="AC1ZCC_001844"
     CDS             complement(4439..5158)
                     /locus_tag="AC1ZCC_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIQ1630090.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5285..6556)
                     /gene="celB"
                     /locus_tag="AC1ZCC_001845"
     CDS             complement(5285..6556)
                     /gene="celB"
                     /locus_tag="AC1ZCC_001845"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HIQ1630091.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            6709..8106
                     /locus_tag="AC1ZCC_001846"
     CDS             6709..8106
                     /locus_tag="AC1ZCC_001846"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HIQ1630092.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8187..8627
                     /locus_tag="AC1ZCC_001847"
     CDS             8187..8627
                     /locus_tag="AC1ZCC_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIQ1630093.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            8775..9101
                     /locus_tag="AC1ZCC_001848"
     CDS             8775..9101
                     /locus_tag="AC1ZCC_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIQ1630094.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9102..9851
                     /locus_tag="AC1ZCC_001849"
     CDS             9102..9851
                     /locus_tag="AC1ZCC_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HIQ1630095.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(9931..10386)
                     /locus_tag="AC1ZCC_001850"
     CDS             complement(9931..10386)
                     /locus_tag="AC1ZCC_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HIQ1630096.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(10813..11088)
                     /locus_tag="AC1ZCC_001851"
     CDS             complement(10813..11088)
                     /locus_tag="AC1ZCC_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630097.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11382..12197
                     /gene="thiD"
                     /locus_tag="AC1ZCC_001852"
     CDS             11382..12197
                     /gene="thiD"
                     /locus_tag="AC1ZCC_001852"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HIQ1630098.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         3902 a         2116 c         2491 g         3709 t
ORIGIN      
        1 atacttggat tgttggcagg gatgaaatta aaaaaagcat tcatgtctgc tttgacttta
       61 gggattgctt ttagcggaat gtctatggtc attggctata tgtctaacgc agtaagtcca
      121 gcatcagaag cattggctaa aaatacggga atcagtttgc ctgcacttga tcttgggtgg
      181 acaggtgcag caagcatcac atggtcgtgg agttatgctt ttatattttt tgcagtaacg
      241 attggggtca attttgtact gttacttttt aattggacga agacgttaaa tgttgatatg
      301 tggaatgttt gggggaaagc tttaactgct tatttagttt attatgtttc cggtagtttg
      361 gctgcaggct ttcttactgc aatggtccaa gttatattgg aattaaagct tggtgatatg
      421 tttcaaaaac atatacaaga tctcacaggg attccattag taacagtgac tcattttatg
      481 acatctgccg ctgtcttgtt gctgcctttc aatatgatca tggataaaat tcctgcttta
      541 aataaacgag cggatacgaa tgcattgaag aagaaaattg ggatcttctc tgaaaactct
      601 gtaatgggat tcattattgg attggggctt ggctttgctg ctgcgtatgg cgtttcaggt
      661 tctcttaatc tagcgatcca aacagctaca gcattaagtt tgtttcctat gatctctaaa
      721 atgttcatgc aatctttgtc tcctctagca gacgctatgt cagaatttat gaaaaagcgg
      781 tttaaggatc gtgaagtata tatcggattg gactggccga tattagctgg tcgttcagaa
      841 atttgggtta ctgctatttt acttgttcct gtgtttattg gttatgccat tattttgcca
      901 ggaaaccaag tattgccatt agcagggatt atcaactatt cgattgcggt cggcggatta
      961 cttttaacag gaggaaactt ggctcgtatg ctgatcttgg gaatcatcac tatgcctatt
     1021 tatttgtacg gggccactta ccttgcgcca atactcagtg atttagcaga aaaaagcgga
     1081 gcagtcgagg gaatcaagaa aggacagttg atcacatggt catccataga agggccggag
     1141 ttcagggttt tatttgcgga ggcatttaat aggaatattc tcgctattgt aggatgcgtg
     1201 atttttctag gactgtttgt tctactttat aaatatatga taaaagcgaa agtaccgagt
     1261 gaacgttatg ctgaactaca aaaatagcat tgaaatggtg ggaaagtaat gaaaagagaa
     1321 gagacagata aaataaaatg gacagtcgct ctttgtggta cacttctgct atttctatat
     1381 ggactcttta ctcagaatat catcatcaat ctattggtca tttttttcgc actagtgatc
     1441 tataaatatg gaaatcacgt tttattcaga gagtacgatg aaaaaagaaa acggaaaata
     1501 gaagaatcaa tgaaaataaa agaagctacc aaagaaatat tgagagaaaa aagttttata
     1561 aaaagataat ggaggggtca ttatgtcaga tggacgtatg ttatttgaga gagaacgaca
     1621 ggacatggca cgtatcgtaa gagtgatttt cgaaaggaaa aacacaaatg tcgcaggagg
     1681 gaatttttcc tttaaaacca ctgatgatca agggaaagac tacatcatca tgacgccgac
     1741 catgatgtca gaagcctatc taggagagat ttctcctgca caggttctag tagtagaacc
     1801 aagaacgcgt agaatcgttg acggagtcgg tcatttaact cgtgaaatca atatgcatga
     1861 agcgatgtat gagactaatc cagctatcaa agctgtccta catgctcatg cgcctaatgc
     1921 aatgttctgg gcgactactg ggttgcctat gcctaatttg acagaagcca cagctaaagt
     1981 aaaagagatt cctgtcatgg actttgagcc taattgctct gaagaacttg ctgaattgac
     2041 aagcgactat gtaaaaaaac agcatctcga attaccgcat atgttgctgc tcaactctca
     2101 tggcgtattg atcaacacag gtggagaatc aggaatcgaa gctatccata aagcactgca
     2161 aatccttgat acagttgaat ggaatgcaga aattgcctac aagcagacga tttttcaagc
     2221 attagggatt actgacggtt tttattcaaa aggtgagaaa atcgggggat tggaagagtt
     2281 gaaacataaa acggctattt ataataaatc tgttattgca gctggtggag actaacctat
     2341 ctgacacaaa aaactacgac tttcacaaaa tctgatccag atcgatatct gcttcaggtt
     2401 gtattatgta agtcgtagtt cttttctttt atttagttaa gaggtggtct gtccattccc
     2461 tcgcttgctt gatttcgtcc atcgtcaact gatgacctgt tacagttagc tgaatagtca
     2521 catttgcttg gcgtgtttcg aattgatcgg ccaattcttt aaaggaagca ggggaaacga
     2581 ttggatcgtt atcaccataa gaaatccaga cggttttatc ctctaatgtg gaacggcttt
     2641 gatgaacacc tagagacatc ggatggaaaa acaacccttt atttagctcg gtctcacgct
     2701 ccaataataa atgagcagcg atgtttgcgc catttgaata gccgacaagc agccagtcac
     2761 ttaacggtat cagttctttt tcgctgattt ctttgatttt ttcatggagt ttatctgttt
     2821 ctttttccaa actgtccagg tcgaattgat tcaatccatt acgcttgaaa aaacgattca
     2881 tcccatcttc ttggaccgtg ccacgaaaag aaagcaatgt actgtttggt gcaagaaagt
     2941 gagcgatatc cagtaaagaa tgttcatcgc cacctgtgcc atgaagtaag atcaaatgct
     3001 tgccacttgt tcctttttca aaaatgtagg tcattttgtt ccctcttttc tgtttctata
     3061 gatgcaaatg aagtagcatg aagagattat tcacgttttt tatttgcatc acttgtatcg
     3121 aaaggacgga cataagcttc aatggcttca cgacgttctt ctaagaatgg aggaagagat
     3181 agtttttctc ctgctgattc tgctggttca tcttcaagga atccaggacc atcagttgct
     3241 aattcaaaca gtacgtgatg agcggctagg aagtactcag attggaaata gaaacgatcg
     3301 acgaaaccag aatttggcaa ttcaatctta ttgattactt gaatccagta ttccaatgct
     3361 tctttgtctg ctacacgcaa tgctaagtga tggatatttc caaatccttc gattgctttt
     3421 ggcaagtctg tccgatgttc aataatgatg cttgcaccat ttcccccttc accgatttca
     3481 aataagtaga aatctcctga atggtctgtt tctttgaatc ctaagatttt ttcaagaatc
     3541 aagcggatat gctctaattg actgacacgg acaaagacag gtccaagtcc tgtgatcgca
     3601 aactctgtcg gtacattgct gtttttccaa ggaatgccag ctgctacacc ttgattgttt
     3661 tcgtcagaga tcaattgata atactgatta tcgaaatctt caaattcgat atattttttg
     3721 ccgaaacgtt cattgattgc tgaatgagta acgtcatatt tttcaaaacg ttccaaccaa
     3781 aagtctaatg ctgcgtcact tggcacacgg aaagaagcac gagagatcgt atctgttcct
     3841 ttttttcctt ttggctgatt tggaaaatca aagaaagtca tatctgttcc tggcgcacca
     3901 gtatcgtctg taaagtaaag atggtaagtt tcgatgtcat cttggttaac tgtttttttg
     3961 attaagcgca ttcctaaaat atctgtaaaa aagcggtaga tttttgtagc gctagatgtc
     4021 attgctgtga cgtggtgaat tcctctgatt tgtgtatcca tgataaattt cctcctgtta
     4081 acttacaaaa agtaagtagt tgatttagac atagtataga gggtgcatct ctattttgca
     4141 attaatttgt cttctaaagt atcaggaagg aatcaagaat ggaaaagagg agggacggat
     4201 caataaaata aaaagactta cgactagctg tatcgatttc aaaaaaacta gctttaaaaa
     4261 gctaacttta gaatcattac gggtggtcat aagtcttttt atgctgagtt attttttcat
     4321 ttttgagcta atgcttagga tagaatagaa tgttgatttc acgatctctt cttgttactt
     4381 gaaactgctt accactgtca cgatctcttg gtaaacggct ttttcacaac ctctttggtt
     4441 aagttgaacg cacatccagc acagaatagt tccgaacatc aaatcggttt cggctggaag
     4501 aatattcaat cggctgtcca tctttgagat agacgacttg ctgaacttcc agtacaggat
     4561 cgttttctgc acagttcaaa tattgctgat catattcatc acttttatct gcagaaatag
     4621 cacgataagc ccctgcaaaa tggacattca attcttgatg aagatgatca tagatagagg
     4681 ctaacaaatg ttcttttttt aaacccggca caagatggac tggcataaag gtatgctcta
     4741 aaacatatgg ctcgccatct aagatcctta accggtgaat attgtagaca ggttgctcgg
     4801 catcgatcat caatcgttct tggatatatt gattagggaa ttcaacatca aaatgaatga
     4861 tttgactctt caaagaacga tgtctgcgtt tcatttggag actcaaacct tcatactcgt
     4921 tagcgggcga tgtgtctttt tgcataaatg gatgattcaa gattttcgtt cctgatcctc
     4981 gctgcgaata gacaagtcct tccattgcta agatattgat ggcttttttg attgtcatcc
     5041 ggctcgcacc aaattctttt accaagtcga cttgattagg caaaaaacta tcgagaggat
     5101 actcttttga acggatacgt tctcttaaaa tatttgcgat ttcttcgtat tttggcacgt
     5161 tattcacctc ttctagagta taacgaaaga ttatgcttcc gacaactcga agtagcatct
     5221 ggaacattgt ttattcgaaa aaagaggtca tgacaagttt ttgtcacaac cccttaatga
     5281 tctattaagc aatatctaaa tctggttgtt tatctagtac tttcaggaat gggtaccaga
     5341 taaaggcaac gatgacaaag tcgatgactt gcagcatacc accaagccat gaatttgttg
     5401 ctaagacacc gcttgcaatg attggcacag tccatggaac agagacgcca gttggtgcag
     5461 ggacaagtcc agcagccatt accaaataat tgaacgtcgt tacgattaga ggtgccacaa
     5521 cccaaggaat aaggattgtt gcattcaata cgatcggtag accgaagatc gctggttcat
     5581 ttacattgaa aataccaggt gctaaagcta atcgtccaac atcacgatac tgtttctttt
     5641 taagaacaaa ggccatcaat aaaacgacag ctaaagtcat tccagagcca ccaataccaa
     5701 cagtgaaggt ttccatgaat ggttttgtga tgatgtgtgg taattctttt ccatgttgat
     5761 aagcttctaa gttatcaagc atcagtgtat tccaaatcgg gtccattaca gagttgacga
     5821 tgatttgtcc gtgaagtccg aagaaccata agaattggac aaagaacaaa gcaatcaatg
     5881 ttgcaggtaa actacttccc aaaccagtta atggtttttg gatcacttca taaacgacgt
     5941 catgtaagtt agcattgaaa ataccagtca tcacagcatt gattactaag aaaatagtta
     6001 gtgtcactac agcagggatc aaagcggcaa atgattttgt tactgcaggc ggaactccat
     6061 caggcatttt gatctgccag ccgcgtttcg tgattctgca gtagatttct ccagccaaga
     6121 aagcagcgat cattccaatg aacattcctt ttgcgccaag acgatcaagt gaaagcacac
     6181 cagtcacatc ttcaccgcca gcggttttca ttgcaaaagg cgtaagaatc aagaagctgg
     6241 caaaagacac ggcaccaccg aaaatacctt caacgtcata agattttgat aaataatagc
     6301 cgataccaaa agtaacaaaa accgacatga tgctcattgt tgcattttgc ccattaccga
     6361 ataaattcga taaagttccc tttgttgcgt cactgaaaaa aggcaagtta ttgataacga
     6421 caacgatcga accgaacatc gttaaaggga aggaaagcat gaaagcatcc cgcagcactg
     6481 taaggtaacg attttgccct aataaattag ctaagggcat gattttctca gaaagcttgt
     6541 ccataaaacc gttcattttt attcacctca aatttatttg caatcgatta catacaaagt
     6601 ataacgccaa aaattttaaa tgtctagtca ttttcataaa aagtattgat ttatttttta
     6661 gggttcgtta tgattacggt atcaagaaaa aagagaggaa gtaaaaaaat gaaaacctac
     6721 acattcccag aaaatttttg gtggggttct gctgctagcg gaccgcaaac agaaggaaga
     6781 gtaagtgatg atggtaaagg agaaaatatt tgggatcatt ggtatgaatt agagcctgaa
     6841 aaattcttcg atcaagtagg acctgaaaaa acttcgcaag tttacacaaa atacaaagaa
     6901 gatgttcagt taatgaaaga aactggccat aacacatttc gtacatctat ccaatggagt
     6961 catttgattc cagaagggac aggtgaaatc aatcaaaaag ctgtttcttt ttatcgagat
     7021 tattttactg aattgctgga taatggtatt gaaccttttg tgaaccttta ccatttcgat
     7081 atgccactac cactgcaaga aaaaggcgga tggttatcaa gagaaacggt cgacgcttat
     7141 gtttcttatg cagcagcgtg ctttgaactg tttggagatc tggtcaaaaa atggtttact
     7201 cataatgaac caatcgtacc tgtagaagga ggctacttgt atcaattcca ctatccaaat
     7261 catatcaata tgaaagaagc ggtccaagta ggctatcatg agaatctagc aagcgcaaag
     7321 gtaatcaaag cctaccatga aggcggacat gatggtgaga tcggaatcat cttgaatctg
     7381 acaccaagct atccgagaga tgaaacgaac ccagaagatg taaaagcggc gaagatcgct
     7441 gacgcattct tcaaccgatc attccttgac cctgcagtga aaggagagtt tccacaagag
     7501 ttgatcgaaa ttgtcaaaga attggctata atgccagaga tccatgaagg agacctagaa
     7561 ctcatccgtg aaaacacgat tgacctgcta ggaatcaatt actatcagcc tcgtcgaatc
     7621 aaagcaaaag aaactccaat caatcacgaa catagcccca tgccagatga ttattttgat
     7681 aattacgaca tgcccaataa aaaaatgaat ccatatcgtg gatgggaaat ttacgaaaaa
     7741 ggaatctatg atattttaac gaatacaaga gaaaactatg gtaatatcaa atgctatatt
     7801 tctgaaaatg ggatgggggt cgaaggagaa gaacgatttg ttaacagcga aggaattatt
     7861 gaagatgatt accggatcga gtttgttacg gatcatttga aatacgtcca tcaagcaatc
     7921 caagaaggaa ccaattgtgt gggttaccac atgtggacat gtatggacaa ctggtcttgg
     7981 acaaatgctt ataagaatcg ctatggcttt atcgctgttg atttggcaca agacggtaaa
     8041 cggacgatca aaaaatcagg tcgctggttt aaaacggtct ctgaaaaaaa cggatttgat
     8101 gcctgatatt ttttgataaa acgttcacaa aatgtttgtt ctaacactgt aatcgtgcta
     8161 cactaacagt gaaggaggcg tttacaatgg tagatactta tcgtaatatt ttagtagcaa
     8221 ttgatggttc tgaaaaggca gaaaaagcat ttcttgaggc aatcacgatt gccaaacgaa
     8281 atcaggcaac gttgcacatc ctgtatgtca atgaagtaac aggcaattac tttggtgatt
     8341 ttgcatttgt tacaacaagc cttcaggaag aattggatga ggttgcggaa aaccagatga
     8401 aagaacatcg aaacttggca attgaaaaag gcctgacaga tatcgagaca tatgttcttt
     8461 acggttatcc aaagacactg attgctaatt tccatgaatc gaaagaacca atcgatctga
     8521 ttgtcatggg ggcgacaggt ctgaatgcgg tagaacgtgc actagtaggt tccactactt
     8581 cctatgtagt caaccacgca tcatgtaacg tcatggttgt aaaataaata aattaaggga
     8641 acagaaagca aaaaagcata acgcttcttt tgctttctgt tccttttatt tttttgtaaa
     8701 aaattacttt acctgatgaa gtaatcatgc tattctaatt acaacgacag atggagtaat
     8761 atggaggaaa gaaaatgatt tcaagtgatg gaatacgcgg atacaatgat gcgattattt
     8821 tatcgatcct taacaaaaac gattcttatg gatatgaaat ttctaaaacg attcgtgaga
     8881 ggacaaatga ttcctatgtg atcaaagaga caacacttta ctccgttttt gcccgtttag
     8941 aaaagaatca actgattgtt tcgtatcctg gagaagtgac tcatggaaaa aagcggacat
     9001 attataaaat cactgaaata gggcgtaatc ttttagaaga aaaaaagcaa gagtggctga
     9061 cgacgaaaga cgtagtcgat caatttttgg agggaaatta aatggataca atccgtaatt
     9121 atttagacag tctttttatc ggagtacctc aaagtaccga gatagataag ctgaaaactg
     9181 atttgttggc aaatatggaa gaccattatc atgaattgat gggagaagga aaaaatgaac
     9241 aggaggccat cggtacagta atttcgacat ttggttcaat cgatgaatta ttagaagaac
     9301 tagatgtcga aaaaaaacat caagctgacg agacggaaac aaacacagct tctatttatt
     9361 taagcgaagc agaaaactac tggaaagaat acagagcggc aagtctccaa gtggcatcag
     9421 gtgttctatt cattagctta tcttttgcaa gttttctttt cttttgttca gcaggatatg
     9481 tgttcatggg tatttcttgt ctgattttcg gtatagcatt agctgtcggt ttttttattg
     9541 cttctggtat gaaaatcaca cgtttaaatc actttttgca ccatcggaaa atacctgaaa
     9601 aagtattagc agaagcaaaa gaaaaagaag aagagtatca gcgaagtttt ggcttctcac
     9661 tgattgcagg aattggatta tgtatttttt cacttttccc acttttggct tctttaatgt
     9721 ggtatatgga tggtagtatc ggtgcctcta tattttttgt tacggtagga acgggtgtct
     9781 tcttgatcat ttatggcagt ctagtccgtc attcttatcg tcaattcact caaagcgctt
     9841 attactggtg aatgtattca gatttgtaaa aatattctat ttatccggcg tagaaaaaaa
     9901 gactgtgaca cgcttttgtc acagtctttt ttatatataa taatttaaaa attcatcatc
     9961 taaatccgta gaattttcta gattgcttac ccatgtaaaa tcgttataga cagatttcag
    10021 ttgacttctg cgccattgta cagggataaa aagacgcatg ctatcttcat tgaatccgac
    10081 taataatttc tttccgtcag taatgatggg acgttgtaac aaagtaaaat gcgagacgat
    10141 cagatcgaca agctctgagg tcgtacattg gtcgatttgg tctttcaaat ttttataatc
    10201 aatcgagcgt ttagctaata gatcgtcaaa tccgttttct gttaatgaca ataaataaaa
    10261 tatctctttc cttgttaaag gctgtttagg tgtgaaaatt cgttctctgt aaggcatttg
    10321 atggtctttc agccagtttg ccatcagatg gtaactatgt gtgcgagaag aatagatcgt
    10381 aagcataaaa aaactcctta aatagtccat taatttgtta gatctgttga gataaataat
    10441 aggtaaggag ctgtgcataa gcattgatca aaacaaccca aacatcatta gaaataactt
    10501 tctaaagtct taaacttgcg acgagaagca actagactaa tagaaaaaat gtcttagcca
    10561 tttggcttcc tgtttgtttt tttgcttatg ctgcagctcc tcgggaaagg gttctaacaa
    10621 tttgaagaag gttgatgatt gatctagttt aaattgtgtg aaatttgaaa tctggtgttt
    10681 ttactagatg aatcacctaa caactatgat tataacttat agaacttctg ttttgcattc
    10741 ggttttgtta tagttgttat aaagttttat tttattacag aacattttta attaataaag
    10801 aacagttaga agttaaaatt gctgattcac atagagttta tgacttcttg gaaattcttt
    10861 ttcaatcagt tttttcatct gtttatgaaa ttcttttttt tcagtaaaca taaaggtttg
    10921 ggtatcgtaa ccttcttcat gtaggaaata gattattcct ttttctgtgt tttgcgtata
    10981 agcttttata ctcctgtctt ttttgaaaga acagatcgta tagccatcta tttggtctgg
    11041 tagtcgtttg attttcttca aaatgacttt tttatcctca agtttcattt ttttcacctg
    11101 actttatttt tttcatatgg aaatcatagc gcctttggtt atatgaaaac aagaaggata
    11161 ctgaaatata tataaatcaa atagtggtta tttattatta gaatagcgaa tacaaataaa
    11221 cgaccactat tttctgattt attgcaaatg ttttttcaaa taaatttgat taccaagaaa
    11281 aggtagtggt atatttactg agggatactt tgaaaaacat atgatagaaa cagtaacagt
    11341 attatgaaaa atctttttat aaaaatggag gaatcgattt gatggaagat cagtatgtac
    11401 aagcaatgac gatcgcagga tcagatagcg atggaagcgc agggatgcaa gcagatatgc
    11461 atacattctt tacaagaaat gtttacggtg tttctgtcat gaccgcatgt gttgcaggta
    11521 attcttacgg aatcggagca agtgtgacat tgccaacaga ttttatcgac aaagaatttg
    11581 aactgattgc gaaggacttt caaatacgtg cagctaaaac aggtatgcta gcagattcaa
    11641 agttgattga gacagttgta aaaaattacc aaaaatatga ttttggacca ttagtcgtgg
    11701 atcccgtaat cgtaacgaaa catggaaatc ttttactaga agaatctgct ttgcaaagct
    11761 tgaaagaaaa gcttgtccct ttggccgaag tactgacacc taatttttat gaagcagaaa
    11821 agctgactgg acggacatta gcagctgaag aagattttgt gcaagcagca aaagaactgc
    11881 aagatatggg tgcaaaaaac gttatgatca aaggacatca tgctaaaaat gcgcagcaag
    11941 aaacagtcaa tgactacgta ttgttcgaat caggaaaatc attttggtta aatgcaccat
    12001 tttatcctac cgaccggatc aacggaactg gcgattcgct atctgcatgt attgcagctg
    12061 agttagcgaa aggaaaaaca gttgaacaag cgatccgatt ggcaaaagac tatgtaaacc
    12121 gtgcaattcg tgaagaaata aatgtagggc acaaatatgg ccccatcaat cattgggcag
    12181 ctggtgaatt agagtaaaag aaagaagttg tgaaaaag
//
LOCUS       DBNICI010000087        12099 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.087, whole genome shotgun sequence.
ACCESSION   DBNICI010000087 DBNICI010000000
VERSION     DBNICI010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.087"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(177..518)
                     /locus_tag="AC1ZCC_001853"
     CDS             complement(177..518)
                     /locus_tag="AC1ZCC_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HIQ1630099.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(586..2757)
                     /locus_tag="AC1ZCC_001854"
     CDS             complement(586..2757)
                     /locus_tag="AC1ZCC_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HIQ1630100.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            2968..3825
                     /locus_tag="AC1ZCC_001855"
     CDS             2968..3825
                     /locus_tag="AC1ZCC_001855"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIQ1630101.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(3896..4753)
                     /locus_tag="AC1ZCC_001856"
     CDS             complement(3896..4753)
                     /locus_tag="AC1ZCC_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIQ1630102.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(4738..7440)
                     /locus_tag="AC1ZCC_001857"
     CDS             complement(4738..7440)
                     /locus_tag="AC1ZCC_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HIQ1630103.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(7453..8742)
                     /locus_tag="AC1ZCC_001858"
     CDS             complement(7453..8742)
                     /locus_tag="AC1ZCC_001858"
                     /EC_number="3.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HIQ1630104.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            8892..9938
                     /locus_tag="AC1ZCC_001859"
     CDS             8892..9938
                     /locus_tag="AC1ZCC_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIQ1630105.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            9940..12093
                     /locus_tag="AC1ZCC_001860"
     CDS             9940..12093
                     /locus_tag="AC1ZCC_001860"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HIQ1630106.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         3330 a         2556 c         2208 g         4005 t
ORIGIN      
        1 caaacatgag aagctgtttt ttgaaattgt tccttgtaac tcagagttaa acggtaggct
       61 cacgacctct tggtaaatgg tgttcaagag acagcctatc ggcaataaga agcaattttg
      121 tcatcgctca aggccgaacg tctctttcac accctctgtt gtttcataaa atagttttat
      181 tcgctgtaaa ggtcgcgaac tggtgtcatg atgatacggt tcaagtcatt gatgattgta
      241 ctgaagcctt gctctttttg catcaagtca ttgattactt cttctgattg gattttcaca
      301 gccatttctt gtgctttcat tgcatcttcg tcgctgaact cttgtccact catttgtttt
      361 tcttgtaatt cgatttgcat cgcttggaag tctttgaaca gtttgtgtgc ttcttcgttt
      421 gcttgtactt tctcgtacgc ttctttcaac gctttgaatt ctggtaattc acggatctca
      481 cgttcgattt gattcgcgct atcgtagatg tttgacatgt tatgccctct tttcttttag
      541 tagttactct ctattgtaac atggggttat tcgttttcaa aggggctact ctccttgata
      601 tttccgccag aggtctttag ccgcttcacc aattccctga acgccttctt tcaccatatc
      661 gccgaagttt ttcagccctt cttttgcttg ttcaccatat tgattcaagt tatcttgcca
      721 atttccagac tctccattag aatttcctgt gtttccaact tgatctgcag ctaccacttg
      781 tcctcctgtg gcataggcat ctgctacact aaatcctgtt tttgaagtat acggaaggat
      841 gccttctgcc gctgatttga agactttccc gacaacattt ccacttgtcc cttccaaata
      901 gtgcagcttg ctggattctt cgtaacccat ccatgtcgaa atcactacgt ctggcgtgta
      961 accgatcatc cattggtcat ttgcttttgt ggcatcgaaa ttagtttctg tggttcctgt
     1021 tttccctgct atcgtataac ctgcaggatt cgcagcagcc gctgttccat ttgaaaatgt
     1081 accaagaagc atgctggtca tttcatcggc tgtttccttt gatatgacct gttttggttt
     1141 tttgtttgtg ttatcgacga tcaccgctcc agtcgaatca acgattttcg tgataagatg
     1201 cggcgtatac atctgaccat cattggcaaa tgcactgtaa gcacccgcca tgatcaatgg
     1261 agatactcct ttttccagtc caccaagggc taagccataa tacttatcag attttgctaa
     1321 aggcaggccg aattcttctg ctttatcata tcctttttgt aaaccgatct catgcaaaag
     1381 ccatacagta ggcaaattca aactttgtgc aacagcttga tacatcggta cttctccttg
     1441 gtatgtcccg tcaaaattat gcgcatcgta ataactttgc ggttcgtctt tcaagatact
     1501 ggaaggcttg tatccagctt ccagtgctgg tgtatatacg cttaatggtt tgatcgttga
     1561 tcctggtgaa cgacgcatct gtgtagcaaa gctgaatcct ctaaagacat gatctcctct
     1621 tcgtcctact agtgcttgta caccacctgt attcggatct aaagctactg aagcagattg
     1681 gaccatggca ccatctgaag catttgctgg gaataatgca ctgttttgat aagtagcttc
     1741 taattgcttt tgataatcct gatctaaagc tgtatagatt ttatagcctt tattcatgac
     1801 atcttcttca tcaagaccat actcgttaac tgcttcatcg atcactgcat caaaataata
     1861 aggataacga taaccagaat ctgaattatc gtatgtgtca ttaagatacg cgctgatatc
     1921 tgtctggctt tctgcttgtt cttgttcttt tgtgattttt ccgttttctt ccattacact
     1981 tagtactgtg tttttccgat cattcgcatt ttttgggtga tcgatcggat tataaatacc
     2041 aggacctttc agcattcctg ccaaagtagc agcctcagac agcgtaacat tcgttgcatc
     2101 tactccaaaa tactttctgg aagcgtcttg gacaccccat accccgttgc caaaataggc
     2161 attgttaaga tacatggtca agatttcttc tttgctgtac ttcttttcga tctcgattgc
     2221 caaaaataat tccttcgctt ttcgatcaaa cgtttggtct aatgtcagat acgcattttt
     2281 ggcaagctgc tgagtgatcg tacttcctcc gccccctgat gttccgccag acaagaccat
     2341 tcggactgct gcacgggcaa tccctttgat atcaaacccg tgatgctcgt aaaaattcct
     2401 atcttctgtc gagattactg catcttggat atagggagaa atcgcgttta attctacata
     2461 tgttcctttt tgggcgtaca gtgtgccagc ttcttcgttt ttcttatcat aaatgactgt
     2521 cgattggctt aatcctgatt ttaacgtttc gacatttgct tgttttgcta aaataaagag
     2581 gtagatactt gtcactaaga cacagatcaa accaatcagc aaaatcaatt tgttcacatg
     2641 atattttttc cagatgcgct ttcgccatcg atggaattgt ccgagatatg gtctgatcca
     2701 acgccaaaaa ctgatcaaag cagcttttat cttttctaaa atgctttgaa aatccatttg
     2761 cttcttctcc tgtctatttg attcaatcgt cttattttac catagtgaaa gagtgttttc
     2821 ttcctgctta agttgtagtt caatatgttt ttttacgtta gtgcaagtag agtaacataa
     2881 gaatgaacag aaaaatgtaa gttttttctt aaatatgttt taaaaatggt acaattagtc
     2941 tgcgattgca ctgaaaggag tacacttgtg aaaatcacga tcactttacc tatgaaccat
     3001 cctgaaacaa caattcgcga cttgcttgaa aaggaatggt tagttcctag aaaggttcga
     3061 cactttttgc gtatacgtaa aaacgtttgg atcaatcaac aacctgcttt gttccatcat
     3121 gaagtaaagg gcaatgacca gatcacattg ttattcgaag aaacagatta taattatcaa
     3181 gaagttcaat taggagatgc gaagcacatc cgtgttttat atgaagatga gcatttgatt
     3241 atagtggata agcctgcagg aatgaagact catccaaatg aaccgacaga aaacagtacc
     3301 ttactcaatc atttagctgc ttatctcgac actaaacagc aacgacctta cgttgtccac
     3361 cgcttggaca aagaaacaag cggtgccgtt ttatttgcaa aaaatccatt tgtcttgccg
     3421 atacttggaa gaatgttgga acaaaaaatg atctatcgga gatatcaagc catcgtatgg
     3481 ggagaaattg ccaaagatat gaccatcacc aataaaattg gacgagatcg ccatgatcgg
     3541 agaaaaaggg tcgtagatga taaaaatggt cagagcgcct tgacacatgt cactgtcaac
     3601 aaagtacttt ctgaacaaac acaagtctat tgtgttttgg aaaccggacg tacacaccag
     3661 atcagagttc atttagctca taagggacat ccgatcgttg gagaccctct ctaccaaaat
     3721 agaacagctg aacgactgat gctccatgca ttagaactgc acatgatcca tccatttact
     3781 caagaaaaaa tagtcggtaa agctttacct ggattatggg aatagaaata aaaaagaaca
     3841 gagtcttatc agcagataat gataagactc tgttcttttt gtgtaatttg tctgcttact
     3901 tttgatgttc gtgttctgct aacagcaaac taccgataat cccactttca ttcggcagtc
     3961 cccaacgaac gatatattca cttacttgag gtgtttccat ataaccaccc atcaattcaa
     4021 caaattgctg acggattttt tgaaggagat gatcctgatt cattacccca ccgcctaaaa
     4081 taatcttttc aggtgctaac gtcaacgtat agttgatcag tgcttgagca atataataag
     4141 cctgaatagc ccaaacagga tgatcttgcg gcagatcttg ccctttgatc cctgtgcgtg
     4201 cctcaataga cggtcctgct gctaatcctt ctaaacaatc tttatgataa ggacaagtac
     4261 cttcataatg gtcatctgga tggcgtttca cccaaatatg tcccatttca ggatgtgcga
     4321 tccctgaaaa aatttcttca ttgatcacga cacctgcacc gattcctgtt cccacggtaa
     4381 ggtaaataca actatttttt ccttgtgctg ctccttgctt caattcccca tatgcagcag
     4441 cattgacatc cgttgtccaa gcagcaggaa tattgtagcg ttccttgatt gtgccaagaa
     4501 aatcgaattg tgcccaccct tttttgggag ttgccaaaac gtatccgtaa ttatcttttg
     4561 ttttgtcgat accaatcggg ccgaatgagc caatcccgat tcccttcaat tcataacgat
     4621 caaaaaattc aaatacctga tccaatgttt cttctggtgt tgtggtaggg atacttaatt
     4681 tttctaaaat ttctccacca tctgaaaccg cacacacaaa ttttgttccg cctgcttcta
     4741 ttccgccata cattcgtttt cctcctcgaa tccgattgtc cgaatctcat aaggttggat
     4801 tattgggaca accgcttgct tcgttggttc ttcaagcaga ttcaatatga cagtttttcc
     4861 attgtcttta gttatttcca atttttctaa atgactagac atattgaaac ctcttactac
     4921 tactttatca ctaaacttgc tgcgtttcaa agctgtaatt gcaaacgttt cactttttat
     4981 cgttaaatat tgttgttttg aaatcaatgt tccttcatga tgtttgattt gttgagtgga
     5041 aaaaggaatc tgtgctgcgt atgcatgcaa ataagtagag aatttttctt ctggtccatg
     5101 tagttcaatg ctgtaattga accgatgctc acctagacac tgtgcttctg gtgttggaaa
     5161 atatccccaa tcgcctagtt ctcccacact gcgaagtagc gtcactgcga tttgtccgtc
     5221 ttctgttact tcatattcat tcaaaccaaa gtttccgact gttacaccgt actcttcatc
     5281 atgaatattg acaaatgctt gctgatgttg cggattcgtg gggttttccc aacttttaga
     5341 cacatggttt gggcgcttca ccacttcaaa aatactatct gcttcgtgat gttccacatg
     5401 taactttgta ggaaacaaga cacgtaagcg atgatctttc atctgattgt cgagagtcgt
     5461 ttcaaagtcg atttttttac tatcttttct gattgtcatg attgttttta tttgaaggat
     5521 tcgtttttct ttggatcgtt ctgcttttcg ataacgaaat tccacaacca tctgctgttc
     5581 tttttctaaa cgctcgtctg cagaaacagg gatttccatt tcctgtagaa tacttatttt
     5641 tgctatttcc ggtgtatcta ctaatacttt tacttcagag ttttctttgt tactggatag
     5701 gattggctga tcacagaaag gttgtttaaa gatatactca tttcctatat cgccgatatc
     5761 ttcaaaaata tgcagattat tcaactttcg tttgtttttg cgatcttcca ttgtaatggt
     5821 accatttttt tcaatcgtca aatgtagagg gccgttttcg atgattcgtc cactctgatg
     5881 aatcattgtt tccttttctt ctgctttcgc ttccccttct acaagcgcga atgtttccca
     5941 agaaaatgct ggcatgtttt ctaatggtaa agtcactttg atcattcttg ccatatatgg
     6001 cacacggaaa cggtctttcg gcaaatcata accaaattgg acagtttctt cagataaaat
     6061 agctggtata gtggccccac tttttgtttc tacatgatac ttacgtttag gcaaattttc
     6121 taactcttga tatagttgtt caggtattcc ttcagcgaat ctttttcttt ctaattcgat
     6181 ggttatttct gcctcaccag ttttatccat tcctgcagta tttacaatga caaatggaaa
     6241 gctgtctttt gggaatccgg tcgtatcgat tgctgcggtc agctgttcta aggaatcttg
     6301 tgcaagatac tttccaactt catctgcttt ttcaaaacga ggtatcattt cccgatggac
     6361 agaatcgact gaacaaccgc aaatactgtc gtgaggatga ttttgcatta atgtcttcca
     6421 agcataatct aattgatcat ggggatagtt tccactaact tcataagcca tcgtcgcaag
     6481 tggttctgta atattttcta gctgtcgctg gaccttggta ttccattgtt ttaagtaaac
     6541 ccgagcagat gctgtattgg ctaaagtata ccagccatcc gtttcttgac ttgtcaattc
     6601 tccttctact gagcctaagt cttctggcac atctttttga actgcttcca gatagtcagt
     6661 aaaattagaa tgaataaact cgtaatctgg aaacagttca tttgcgagat ggattgcttt
     6721 tgatatatct ttttgtaccg gttgatgatc aacaccattc atcatcaata agtggttcgt
     6781 agaggcatat tgttctgcat ccgctaattt ttgcttccaa aaggcaagag ctgcttcttt
     6841 ttctactgga atctcattcc cgttgctata ccaattggca aatagcagac caaagattgc
     6901 tgtttgatca ggtcctttcc accacatctc tgagtattgg gaagaatagt tttctgcttc
     6961 caaaacttga ttatcaaagc caattggttt tacaccacgg ccaaacgctg ctgctgaaat
     7021 accagcttgt ttcattaatt gaggcgtctg tcccatattt ccaaatgtat cgggaaaata
     7081 gcctaacatg acaggggttc cccatttacg gctttcttcc atgccaatca gcatatttct
     7141 cacgttcgat tccgagctga taagaaaatc atcttgcaag atataaaatg gtccaattct
     7201 caattttcct tcattgattg cttgttggat tgcctgtctt ttttctggcc ttacttgaag
     7261 atagtcatct aaaataatcg tttgcccatc caaatgaaaa ctgttgaagg acggatcaac
     7321 ttcaaatagc tctagtagat cgtctatcaa ttcaatcagt cgcatgtgat gctgttcgta
     7381 agccatgtac cattcacgat cccagtggct atgagaaatg atatatactt tctttttcat
     7441 ttacgatacc tcctactttt ctactctaat atcaaagtaa tccatgacta attcgcaaaa
     7501 catcatattt gcccaagaaa accattctct cgtataattt tcaggattgt tcacatcgaa
     7561 tccttcgtgc ataaggtgtg ttcctgcgtc agttgctaca agtaaattca atatccgctc
     7621 tttttcagat ttgtctgacg ttgtcattcc ttccatagct aatgcaatcg gccaaacgta
     7681 tttttctggc gtatgggagg atccaattcc ttttgcgtac tttccttcat agaaatatgg
     7741 attttcttta ctcaacaatg ttcttcttgt ttgcagataa tcttcctctt gttcagaaca
     7801 atatcccaag taaggtgcgg cgatcagatt gggcacatta ctgtcatcca tgactgtggc
     7861 gtttcccatt ccatctactt catatgcata aatcgtttgc cctttttgat taaccgttcg
     7921 tccgtgattc tgaatcccct cttggatctc tttcttcaat ttctgtgttt gctgtacaat
     7981 ttctttatca tcgagtatcg tttgaaaaat ttcttccaca taacctaaca cgaccaccgc
     8041 aaacatattg gatggtacca gatatccata acggcaagta tcatcgctag gacggaaacc
     8101 agaccatgtc attcctgtaa aagctgtttt tgcacctcgc ccgtcattga tcagcgtatc
     8161 ttcttttctt gtcgtgtctc gtacaaatgt ataaggagag tgttcatgat cttgctctgt
     8221 tttgaacacg tccaagattt ttttgatacc ttcaataaaa gattgattga attgatcagt
     8281 ttttcctgta tttttgtaaa gaagataggc caactgtatt gggtagcata aagaatcaat
     8341 ttcatatttt ctttcccaga tccaatcgtt catttttgta tgatctgttt ggtggccagc
     8401 accgttagct tcttcgttga aggcattcgc atatggatcg atattgatat aaaaaaattg
     8461 ctttttgact aacccgctga tcatgtcagc taattcctca tcttcttttg caatcactaa
     8521 ataaggacga acttgtgcag tagagtctct taaccacatc gctggaatgt ctcccgtcaa
     8581 taaaaaggtg gttccgtcat tttgcttttt gactgttgtc aacagagtat tggcaaatgc
     8641 tgctgtaaaa ttctttgccc agtccgcatg gatatcccca catttttgtg tgatatcttc
     8701 cataaatttc tgcaccgatt ttggtatatc tttataagcc actaataaaa ccttctttct
     8761 atttgatata tctttaaaga ttatacttca tacagccaat tatgtctacg ctttcaaaaa
     8821 atatcttttt atttactttt tcatccatta tggtattatt tgaatatgat atatcattaa
     8881 gaggtgttta tatgaatcaa ccattgtacc agacaatatt taatgatttg caaacggcta
     8941 ttttaactgg tgaattacct gtagacagcc aactaccaac tgaaaaagaa ctttcacgta
     9001 cctataaagt aagcagaatc acttccaaac gtgcattgac tgaattagag caattaggac
     9061 ttatttatcg cgttcgcggg aaaggcagtt tcgttaaatc cgcttctccg gaacagattc
     9121 ttgcttctcc tacaaaaaca cgacggattc tgtttcttct tccatatgtc gctgatttgt
     9181 ctgttggtga ttttagcaag ggattgaacc ctgtgatgca agataaacag attgatgtca
     9241 tgatgacgac gcttgacttt ctacaaaata aaacagcaaa agagctaatc aatgaatttg
     9301 acggattgat ttattatgct cttgatacag aacaacatct tgacttactt tttgagcttt
     9361 cattgaaaga attccctgtc gtcatattag ataaaaaaat ctatgagtta ccttttccta
     9421 ccgttctttc cgataatttc caaggcggtg cgcttgctgc ctcttatttg atcgaacagg
     9481 gacattcaaa aatcgcttat ttgtttggta cgcagactca tccacaatcc gttcgacagc
     9541 gttaccttgg ttaccttgaa gcattggaca aagctggttt gactttccac acttcactgg
     9601 atgacaaaag cgcgacatta gatgaattgc tttcttatat cactaagtat gaagtaactg
     9661 cttttgtttg tgaaaacgat gttgtcgcta tccagaccat gaatctcttg agacagcatg
     9721 gttatcatat ccctgaagat ttttcaatta tcggatttga tgatatccaa gcagcagctc
     9781 ttgttgaccc gccactgaca accatcgcac aagattttga acagttaggt aagcttgcgg
     9841 gagatgcttt ggtcacttgg ttagagacaa aagatcgacc atccgatagt aaatttccag
     9901 tttcattcat caaaagacaa tcaacaaagg agaaataaaa tgaatatcgg tcaaatagat
     9961 actagacatg caacagcgaa tcagccaaat ttctctaatg ggaactgcca gccttatact
    10021 ggcgttcctt ttgggatgaa ttattttgca ccgcaaacta cggatcaaaa cggcagctgg
    10081 tggtttcatc ctgatgaccg cgtttttcaa ggctaccgtc ttacccatca accaagtcct
    10141 tggatgggtg atttcagtca tatgctgttt acgccgatca atggaaaact acaggagaac
    10201 acccttttcc atgctcaaag ttcttaccgc ccagaagaaa gtatattttg cccgactcat
    10261 ctgtcgatct gtcaattacg ttatggcatc tgttctacat tgatacctag catgtatggc
    10321 ggaatcttat ctattgatta cagcaaaaat gacagcggtt tacttttatc ctttccggga
    10381 cgccatcagc tctttgttat agatccttac acagtggaag gaaaaatcac acattttgct
    10441 ggttcagaag atcctgattt ttctttttat tttatattga gatttgaata tcctttaaca
    10501 attgatgagt tagctgtttc caacaaaaat agcgactcga tcccgttgta ttttgaacag
    10561 acaaaaaaac aaactatccg atttgggaca tcttttatca gctcggaaca agcagcttat
    10621 aacctggaac tagaacaaaa ccaaagcaaa gagacttacc ttgctcacag tcaagaacaa
    10681 tggcagtcct attttgatcg tatacagctc acacaccatg atcaacaaca attgcggacg
    10741 ttttatcata atttatatcg ggtgttttta tttccacaaa cattttatga aattgatcct
    10801 agtgggcaaa aaatccacta tgatacgact agtcgatcta taaaacctgg tgttctctat
    10861 acaaataatg ggttttggga tacgtataaa acggtttatc ctctttattc tttgattgct
    10921 gtagaaaaat acgaagaaat gctggaaggt tttttgaaca gctatagaga aagtggctat
    10981 ttgccaaaat ggctgtctcc tgatgaacgt ggacttatgc ctgggacatt gatcgatgcg
    11041 gtcattgcag atgctgctgt aaaagatatc cgacgagact tgatgcctga atttctaacg
    11101 gcaatggaaa aaggtgctac ggttcaaagc aaacatccaa attatggacg ccaaggaaca
    11161 gaagattact tgacatatgg ctatgttcct aatcactacc acgaatcagt caatcacaca
    11221 ttagactatg cttatagcga tttttgtatt agccaagttg ccaaggtact agaagaagag
    11281 gaaatgacga agaaatatca gcagcaatca ttgaactatc aaaatatctt tgactatgag
    11341 tcaggattta tgcgttcaaa agataaagat ggcaagttca gagaaccatt ttcagctaat
    11401 cgttggggac aagattatgc agaaggaagt gcttggcaaa gcagctttgc tgtttatcac
    11461 gatttttcag gactgatcgg aaaatatggt ggcagtgaga gatataaaga aaaactcatt
    11521 gagctgtgca atcagccccc aacgtttgac ccaggaggat atggttttga gatccacgaa
    11581 atgagtgaga tggccgcgat agaatttgga caattagcta tttcaaacca gccaagtttt
    11641 cactatcctt acttgttcag ttatatagga caacctgaaa tggcacagcc actgcttaaa
    11701 cagttgatga ctcagacctt cgacgattct ccaatgggtt accctggaga tgaggacaat
    11761 ggaagtatgg ccgcttggta tatcttcaat agtctgggat tttatcctgt cacgcctgga
    11821 accggtgaat atgttatcgg tatgccatta attgacaagg ctgttttgct tttgtcaaat
    11881 ggaaaggaat tgacgattga agcaacgcct aatcggccgc agcaacaatt tatccataaa
    11941 atcaaacgga tggacaaaga atatacgaaa ttatatttta cccatcaaga tttgctgaaa
    12001 ggcggcacga tctcttacca attagggatc atccctcacc ctaagaaata tcaagcaaaa
    12061 gatttgcctt tttccttatc aaactataat taacaagag
//
LOCUS       DBNICI010000088        12074 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.088, whole genome shotgun sequence.
ACCESSION   DBNICI010000088 DBNICI010000000
VERSION     DBNICI010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.088"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(133..1440)
                     /locus_tag="AC1ZCC_001861"
     CDS             complement(133..1440)
                     /locus_tag="AC1ZCC_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HIQ1630107.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(1443..2024)
                     /locus_tag="AC1ZCC_001862"
     CDS             complement(1443..2024)
                     /locus_tag="AC1ZCC_001862"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HIQ1630108.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(2140..2237)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(2596..3888)
                     /locus_tag="AC1ZCC_001863"
     CDS             complement(2596..3888)
                     /locus_tag="AC1ZCC_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIQ1630109.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(3885..4508)
                     /locus_tag="AC1ZCC_001864"
     CDS             complement(3885..4508)
                     /locus_tag="AC1ZCC_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630110.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(4511..5347)
                     /locus_tag="AC1ZCC_001865"
     CDS             complement(4511..5347)
                     /locus_tag="AC1ZCC_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1630111.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(5364..6251)
                     /locus_tag="AC1ZCC_001866"
     CDS             complement(5364..6251)
                     /locus_tag="AC1ZCC_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1630112.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(6267..7550)
                     /locus_tag="AC1ZCC_001867"
     CDS             complement(6267..7550)
                     /locus_tag="AC1ZCC_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIQ1630113.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(8233..8916)
                     /locus_tag="AC1ZCC_001868"
     CDS             complement(8233..8916)
                     /locus_tag="AC1ZCC_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HIQ1630114.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(8934..9551)
                     /locus_tag="AC1ZCC_001869"
     CDS             complement(8934..9551)
                     /locus_tag="AC1ZCC_001869"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HIQ1630115.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(9561..10385)
                     /locus_tag="AC1ZCC_001870"
     CDS             complement(9561..10385)
                     /locus_tag="AC1ZCC_001870"
                     /EC_number="3.4.-.-"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HIQ1630116.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(10378..10938)
                     /locus_tag="AC1ZCC_001871"
     CDS             complement(10378..10938)
                     /locus_tag="AC1ZCC_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630117.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            11112..12068
                     /locus_tag="AC1ZCC_001872"
     CDS             11112..12068
                     /locus_tag="AC1ZCC_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIQ1630118.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
BASE COUNT         3631 a         2653 c         2046 g         3744 t
ORIGIN      
        1 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
       61 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
      121 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
      181 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
      241 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
      301 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
      361 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
      421 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
      481 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
      541 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
      601 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
      661 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
      721 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
      781 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
      841 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
      901 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
      961 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     1021 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     1081 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     1141 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     1201 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     1261 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     1321 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     1381 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     1441 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     1501 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     1561 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     1621 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     1681 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     1741 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     1801 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     1861 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     1921 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     1981 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     2041 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     2101 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     2161 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     2221 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     2281 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     2341 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     2401 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     2461 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     2521 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     2581 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     2641 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     2701 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     2761 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     2821 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     2881 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     2941 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     3001 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     3061 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     3121 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     3181 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     3241 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     3301 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     3361 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     3421 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     3481 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     3541 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     3601 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     3661 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     3721 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     3781 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     3841 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     3901 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     3961 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     4021 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     4081 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     4141 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     4201 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     4261 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     4321 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     4381 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     4441 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     4501 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     4561 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     4621 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     4681 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     4741 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     4801 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     4861 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     4921 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     4981 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     5041 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     5101 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     5161 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     5221 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     5281 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     5341 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     5401 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     5461 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     5521 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     5581 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     5641 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     5701 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     5761 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     5821 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     5881 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     5941 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     6001 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     6061 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     6121 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     6181 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     6241 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     6301 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     6361 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     6421 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     6481 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     6541 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     6601 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     6661 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     6721 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
     6781 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
     6841 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
     6901 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
     6961 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
     7021 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
     7081 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
     7141 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
     7201 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
     7261 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
     7321 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
     7381 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
     7441 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
     7501 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
     7561 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
     7621 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
     7681 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
     7741 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
     7801 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
     7861 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
     7921 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
     7981 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
     8041 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
     8101 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
     8161 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
     8221 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
     8281 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
     8341 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
     8401 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
     8461 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
     8521 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
     8581 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
     8641 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
     8701 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
     8761 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
     8821 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
     8881 ttccgtcatg atttctggcg taaattgttc tgccatcaac cacttctcct ttgctatctc
     8941 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
     9001 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
     9061 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
     9121 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
     9181 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
     9241 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
     9301 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
     9361 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
     9421 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
     9481 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
     9541 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
     9601 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
     9661 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
     9721 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
     9781 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
     9841 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
     9901 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
     9961 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    10021 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    10081 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    10141 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    10201 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    10261 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    10321 aaaataacat agcccattgc tgcgatcata atcacaactg ctccaaatcc taaaaattta
    10381 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    10441 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    10501 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    10561 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    10621 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    10681 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    10741 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    10801 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    10861 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    10921 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    10981 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    11041 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    11101 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    11161 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    11221 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    11281 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    11341 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    11401 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    11461 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    11521 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    11581 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    11641 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    11701 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    11761 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    11821 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    11881 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    11941 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    12001 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    12061 atatgtaatt ttaa
//
LOCUS       DBNICI010000089        12050 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.089, whole genome shotgun sequence.
ACCESSION   DBNICI010000089 DBNICI010000000
VERSION     DBNICI010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.089"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            168..362
                     /locus_tag="AC1ZCC_001873"
     CDS             168..362
                     /locus_tag="AC1ZCC_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630119.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="AC1ZCC_001874"
     CDS             631..930
                     /locus_tag="AC1ZCC_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630120.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="AC1ZCC_001875"
     CDS             complement(1027..1461)
                     /locus_tag="AC1ZCC_001875"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1630121.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="AC1ZCC_001876"
     CDS             complement(1693..2403)
                     /locus_tag="AC1ZCC_001876"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HIQ1630122.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="AC1ZCC_001877"
     CDS             complement(2393..3253)
                     /locus_tag="AC1ZCC_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HIQ1630123.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="AC1ZCC_001878"
     CDS             complement(3258..3896)
                     /locus_tag="AC1ZCC_001878"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HIQ1630124.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="AC1ZCC_001879"
     CDS             complement(3911..4210)
                     /locus_tag="AC1ZCC_001879"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIQ1630125.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="AC1ZCC_001880"
     CDS             complement(4253..5737)
                     /locus_tag="AC1ZCC_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HIQ1630126.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="AC1ZCC_001881"
     CDS             complement(5757..6218)
                     /locus_tag="AC1ZCC_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIQ1630127.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="AC1ZCC_001882"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="AC1ZCC_001882"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HIQ1630128.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="AC1ZCC_001883"
     CDS             7521..8291
                     /locus_tag="AC1ZCC_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIQ1630129.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="AC1ZCC_001884"
     CDS             complement(8564..9391)
                     /locus_tag="AC1ZCC_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1630130.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="AC1ZCC_001885"
     CDS             9959..11194
                     /locus_tag="AC1ZCC_001885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIQ1630131.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         3794 a         2450 c         1899 g         3907 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg
//
LOCUS       DBNICI010000090        11885 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.090, whole genome shotgun sequence.
ACCESSION   DBNICI010000090 DBNICI010000000
VERSION     DBNICI010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.090"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(3..2714)
                     /locus_tag="AC1ZCC_001886"
     CDS             complement(3..2714)
                     /locus_tag="AC1ZCC_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIQ1630132.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(2724..3425)
                     /locus_tag="AC1ZCC_001887"
     CDS             complement(2724..3425)
                     /locus_tag="AC1ZCC_001887"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1630133.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            3611..4210
                     /locus_tag="AC1ZCC_001888"
     CDS             3611..4210
                     /locus_tag="AC1ZCC_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HIQ1630134.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            4291..5304
                     /gene="rbsR"
                     /locus_tag="AC1ZCC_001889"
     CDS             4291..5304
                     /gene="rbsR"
                     /locus_tag="AC1ZCC_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose utilization transcriptional repressor
                     RbsR"
                     /protein_id="HIQ1630135.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            5412..6329
                     /gene="rbsK"
                     /locus_tag="AC1ZCC_001890"
     CDS             5412..6329
                     /gene="rbsK"
                     /locus_tag="AC1ZCC_001890"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HIQ1630136.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            6357..6752
                     /gene="rbsD"
                     /locus_tag="AC1ZCC_001891"
     CDS             6357..6752
                     /gene="rbsD"
                     /locus_tag="AC1ZCC_001891"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HIQ1630137.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            6752..8125
                     /locus_tag="AC1ZCC_001892"
     CDS             6752..8125
                     /locus_tag="AC1ZCC_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIQ1630138.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            8482..10626
                     /locus_tag="AC1ZCC_001893"
     CDS             8482..10626
                     /locus_tag="AC1ZCC_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HIQ1630139.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(10678..11718)
                     /locus_tag="AC1ZCC_001894"
     CDS             complement(10678..11718)
                     /locus_tag="AC1ZCC_001894"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIQ1630140.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT         3629 a         2402 c         2253 g         3601 t
ORIGIN      
        1 ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt gaaatgggta
       61 acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac ataagcccac
      121 catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa gatgaagtca
      181 gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa gatgatattt
      241 tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt tattgtagat
      301 aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac gataaaagcc
      361 agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc cattgattct
      421 tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc tgtggcaagc
      481 agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg cagcaaaaat
      541 gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga catataaata
      601 aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc tttttgatct
      661 tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc tactttttgc
      721 gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc agttaaatgg
      781 ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact ttgcgaagca
      841 tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc atttactttt
      901 tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga tgaatccaat
      961 gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac agaacttaga
     1021 gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat tccgataatc
     1081 gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt ttgattaaag
     1141 ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc tggcttcggt
     1201 gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa aacgatcatt
     1261 gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg cggccagtca
     1321 taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa aatgatcctt
     1381 ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga ggctagactt
     1441 gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc ctttaatgtc
     1501 ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt gatcaatgcg
     1561 gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg atcagacaga
     1621 tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta tgatggttta
     1681 gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga ggccagttca
     1741 cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg ttgagataat
     1801 gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc tgactgttgc
     1861 gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg tgctttgcca
     1921 tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt cagctcttct
     1981 aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt ttgctccatt
     2041 ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa atcaagatat
     2101 aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa ataatcgacg
     2161 ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa tggagaacgg
     2221 acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat ctgataaata
     2281 tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg gtttggtttt
     2341 tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt gttataagaa
     2401 aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt tcctgtttct
     2461 acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa gccaccagta
     2521 gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat cgttttttgc
     2581 atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat gacaataagg
     2641 atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc tttaagatat
     2701 gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga aggatgatca
     2761 tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc agcgatgggc
     2821 gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct gctagcgttt
     2881 tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt cggttcatca
     2941 cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct ctgctgttcg
     3001 cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac ttgcctcaat
     3061 acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac attttctttt
     3121 gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc tgttcgtcgg
     3181 taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat aatgatctga
     3241 ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt tcctgctcca
     3301 cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat atcgtcattt
     3361 gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac ttcgacatac
     3421 ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat cgaatgcgtt
     3481 ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg aatcctttaa
     3541 tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt tatttgaaag
     3601 aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa aaactgcttt
     3661 tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc cacacactgc
     3721 agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta ccgctgcctt
     3781 gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac aaactgatat
     3841 gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga aggattcttt
     3901 gaaagataca gataatatgt tgactatcac aaaatacaat ccaagtgctt tttttggtac
     3961 ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa gcggtgtagc
     4021 gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt accatatcgt
     4081 gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt tcataaaaaa
     4141 gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag ctatccaaca
     4201 aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt catgggaaaa
     4261 taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac aatcaaagaa
     4321 gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa tggaaatacg
     4381 gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga attaggctat
     4441 cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat tggtgtactc
     4501 gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga ggatatcttg
     4561 tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca aaaagaaatc
     4621 gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc gacttctgct
     4681 gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt cattgttttg
     4741 gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt tcgcggtggt
     4801 tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct tgtctatcct
     4861 gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc tcttgatgtt
     4921 taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa acaaggtggc
     4981 taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc tttaaatgat
     5041 gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat ccctgaagac
     5101 tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc gaaactgaca
     5161 acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt gttagatcgg
     5221 atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt agaaaaaaga
     5281 ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc gaactattaa
     5341 aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg agatagttgg
     5401 aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac actacccttc
     5461 gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt tttcacgctg
     5521 gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca cggacatctt
     5581 ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta ctgacaaaag
     5641 aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga caagcaatga
     5701 ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca aataatgctt
     5761 tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac tttattattg
     5821 ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca aaagacgcag
     5881 gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact gaactacttg
     5941 aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca ggaatcagag
     6001 tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta ggtattggaa
     6061 cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa gaacacggta
     6121 tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat acatttattg
     6181 gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg atcctttatg
     6241 gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct atcccttacc
     6301 gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa acaaacatga
     6361 aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg gggcactttg
     6421 ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa aaaatagatt
     6481 tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt ttagaagaac
     6541 ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg gaaatattgg
     6601 aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat gaagaaatga
     6661 aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca ccttacgcca
     6721 atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc ctgaacagat
     6781 ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag acaatattga
     6841 ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc cagtagccaa
     6901 tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac tcgatagata
     6961 tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga cgcgtcaaat
     7021 cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg gtttgatatc
     7081 ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa aaggtctggt
     7141 tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc atattttgtc
     7201 tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt ctgctgccaa
     7261 aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga ttgaaccgaa
     7321 tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg ggattcctgt
     7381 agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt tcttttattc
     7441 ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag tatttgtacc
     7501 tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc ctgcgggtgt
     7561 ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta acggaccgat
     7621 cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta tccaatgggc
     7681 tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa tcgttccgtt
     7741 gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc tgcgaagtaa
     7801 acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac ttgcgggagt
     7861 gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg ttgctgcttg
     7921 cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag cgatcgcttt
     7981 tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt tctggtactt
     8041 agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta gcgggtttga
     8101 tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag atgtttatca
     8161 catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt gatactttcc
     8221 aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt tatccccaaa
     8281 caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg ctcgttgctt
     8341 atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa aagcttttcg
     8401 acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga aaatcaatga
     8461 aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct agctgctact
     8521 tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga taatcaaata
     8581 gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag tgaaacaacg
     8641 gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc gaccgagcca
     8701 actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac atattccctt
     8761 acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc ggattcccct
     8821 gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt agcggaaacg
     8881 aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt cgctgttaat
     8941 ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa caatttatct
     9001 attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac tgatcctagt
     9061 gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt gggtaataac
     9121 aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa cagcgggaat
     9181 tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca gagagaacca
     9241 aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc tcgtccaaat
     9301 acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt tgttgtaaaa
     9361 tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc aatcgttcga
     9421 gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc aaatactttt
     9481 gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta tatcggtggc
     9541 tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc ccaacctggc
     9601 gacctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat ctatattggg
     9661 aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac ttctatttca
     9721 gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc tgcttcaggt
     9781 tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca tttcagtcaa
     9841 aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga aatcgggcaa
     9901 aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag tggatctgga
     9961 aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg atcatataaa
    10021 ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag ctaccaaatc
    10081 cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta tgccgacttg
    10141 atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa gtcggaaaca
    10201 aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga tcgccaatat
    10261 tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga tgaaccgaaa
    10321 aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc taagcgattc
    10381 gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga attttcaacg
    10441 ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg ggatttgtgg
    10501 aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa agctgcaact
    10561 gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct gcctttaaag
    10621 aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta atttattcta
    10681 tttctttata tttattttgt attccgttac aaactcgcgt acttggttag gttccagagt
    10741 gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct caaatgtgat
    10801 accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc caaaatttgc
    10861 tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt tttcatctgg
    10921 gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa atggatgatc
    10981 gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac ctaatttttt
    11041 aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg taggcgttgt
    11101 atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt cgtcgattga
    11161 ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga atactgtttt
    11221 gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca gcgtataccg
    11281 tacttctaca tctaaagttc caggaaatcc gttttccata tctgggctag tctgatagaa
    11341 aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct tggcttcaaa
    11401 gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta cttcgaacgt
    11461 ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc cgattgaagc
    11521 accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta agatcaactc
    11581 tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa gatcactgac
    11641 agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt cgccaaaatt
    11701 ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac tgattccagt
    11761 atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa aatttactat
    11821 attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca acctcttttt
    11881 ccgaa
//
LOCUS       DBNICI010000091        11860 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.091, whole genome shotgun sequence.
ACCESSION   DBNICI010000091 DBNICI010000000
VERSION     DBNICI010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.091"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="AC1ZCC_001895"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="AC1ZCC_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HIQ1630141.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="AC1ZCC_001896"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="AC1ZCC_001896"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIQ1630142.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="AC1ZCC_001897"
     CDS             complement(2169..2906)
                     /locus_tag="AC1ZCC_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIQ1630143.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="AC1ZCC_001898"
     CDS             complement(2997..3524)
                     /locus_tag="AC1ZCC_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630144.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="AC1ZCC_001899"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="AC1ZCC_001899"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HIQ1630145.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="AC1ZCC_001900"
     CDS             complement(4401..4886)
                     /locus_tag="AC1ZCC_001900"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HIQ1630146.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="AC1ZCC_001901"
     CDS             complement(5039..5236)
                     /locus_tag="AC1ZCC_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630147.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="AC1ZCC_001902"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="AC1ZCC_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HIQ1630148.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="AC1ZCC_001903"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="AC1ZCC_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HIQ1630149.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="AC1ZCC_001904"
     CDS             9211..9423
                     /locus_tag="AC1ZCC_001904"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HIQ1630150.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /gene="rnjA"
                     /locus_tag="AC1ZCC_001905"
     CDS             9425..11104
                     /gene="rnjA"
                     /locus_tag="AC1ZCC_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J1"
                     /protein_id="HIQ1630151.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="AC1ZCC_001906"
     CDS             complement(11358..11558)
                     /locus_tag="AC1ZCC_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIQ1630152.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..>11860)
                     /locus_tag="AC1ZCC_001907"
     CDS             complement(11708..>11860)
                     /locus_tag="AC1ZCC_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HIQ1630153.1"
                     /translation="GNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRYFSLREE
                     GMMEEK"
BASE COUNT         3428 a         2567 c         2064 g         3801 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc
//
LOCUS       DBNICI010000092        11677 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.092, whole genome shotgun sequence.
ACCESSION   DBNICI010000092 DBNICI010000000
VERSION     DBNICI010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.092"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(27..878)
                     /locus_tag="AC1ZCC_001908"
     CDS             complement(27..878)
                     /locus_tag="AC1ZCC_001908"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIQ1630154.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            1001..1468
                     /locus_tag="AC1ZCC_001909"
     CDS             1001..1468
                     /locus_tag="AC1ZCC_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HIQ1630155.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(1731..2213)
                     /locus_tag="AC1ZCC_001910"
     CDS             complement(1731..2213)
                     /locus_tag="AC1ZCC_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HIQ1630156.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(2313..3176)
                     /locus_tag="AC1ZCC_001911"
     CDS             complement(2313..3176)
                     /locus_tag="AC1ZCC_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIQ1630157.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            3774..4649
                     /locus_tag="AC1ZCC_001912"
     CDS             3774..4649
                     /locus_tag="AC1ZCC_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HIQ1630158.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(4794..4952)
                     /locus_tag="AC1ZCC_001913"
                     /pseudo
     CDS             complement(4794..4952)
                     /locus_tag="AC1ZCC_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
     gene            complement(5214..6545)
                     /locus_tag="AC1ZCC_001914"
     CDS             complement(5214..6545)
                     /locus_tag="AC1ZCC_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIQ1630159.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(6694..7407)
                     /locus_tag="AC1ZCC_001915"
     CDS             complement(6694..7407)
                     /locus_tag="AC1ZCC_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIQ1630160.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            7582..9006
                     /locus_tag="AC1ZCC_001916"
     CDS             7582..9006
                     /locus_tag="AC1ZCC_001916"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HIQ1630161.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            9305..10012
                     /locus_tag="AC1ZCC_001917"
     CDS             9305..10012
                     /locus_tag="AC1ZCC_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HIQ1630162.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            9999..10322
                     /locus_tag="AC1ZCC_001918"
     CDS             9999..10322
                     /locus_tag="AC1ZCC_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HIQ1630163.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(10411..11448)
                     /locus_tag="AC1ZCC_001919"
     CDS             complement(10411..11448)
                     /locus_tag="AC1ZCC_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HIQ1630164.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
BASE COUNT         3629 a         2297 c         1915 g         3836 t
ORIGIN      
        1 cggctttttc acaacctctt tatacattat tcctgatcca atatttcttt taatgctgct
       61 tcaagagaag ttaatggttt tcccaatact ttttctaaat catcagagga aatagctaac
      121 tgatcctttt taatatcatg ttggatcgct aaaaacatgt cagctgcttc ttgagggaat
      181 ccggcttctt ttagattttc gacaaacgct gcatcacttg cttcaataat ttctaacttt
      241 ttacctgtcg cacgctctaa tgccttggct aattcttcat agtcagttag attgccggat
      301 aattctaaga tttctggaga gtcactttct actagtactt ttgctgcagc ttctgcatat
      361 tcacgtttca atgcccaacc tgtttttcca tttcctgcag cataaacgaa ttgaccagtt
      421 tttaaagcct gcccaatgat aggtaattca ttctctacat accaattatt ccgtaaaaat
      481 gtatgggaaa tacctgaatc cttaatcatt ttttcagtga attggtgatc tggcgctaat
      541 acactgatcg agtgatcggc atctgcaaaa cttgtataag cgatataaga taccccagct
      601 gcttgtgctg ccttcactac attcttatgt tcggcttgac gatttcctgg aacgccagag
      661 acaaaaagca atcgatctat tcctttcaac gcttctttca ttgagtctaa atcatcataa
      721 tcaccaatac gtatattaaa tccagcttct ttcagtgctt ctcctttttc ttcactacgt
      781 actagtacat agatttcatt ttgtggaacc atctttttta agaattccag cgcataacca
      841 ccaaaacctc cagttgctcc tgttactaaa taatgcatat ttgccactcc ctattctttt
      901 tatttgttaa gatttacagc tgtacattta taaattacac ctttatcata aagagtgtgg
      961 taagataagt caagcaaaaa aaatgaaagg atgacaactg atgaagtatt ctctgcaatt
     1021 tagtgatgca atccatgtac ttgcctatat tgaaatgttt caagataccc gatcactttc
     1081 tagtgagatg attgccagta gtatcgaaac aaatgcagca aatgtacgta aaatcatgag
     1141 tcagctcaaa aaatccggtt tgatctttac aaaaaccgga aaaccgcagc cttcccttgc
     1201 caaaagtcca gatcagattt ctttactaga tgtctatcgc agcatcgaag gtaatacaca
     1261 gcttatccaa gtcgatccaa aaacaaatcc aaattgcgtc gttggtgcga atatccaaga
     1321 agctttagaa gaaagctacg ctcggctgca gaaaaaggca gaagaagaaa tggctaagat
     1381 cacacttgat agtctgatac ataaaatagc cgttttggaa aaagaaaaaa gaccagaaaa
     1441 cacaggcctc gtagagagat ttttataatt ctctctactg tgcctgcatt tttctgatcg
     1501 gaatcgtcta cataaattct tttattctcc gggggacaaa agccttgtca tgcccctttt
     1561 tcctctctaa tgctcaggtc aaagcgctga agtcatgacg tcttgataaa cggtgttcaa
     1621 gtagctgacc ctcggcatta gttctgaaaa atgcaaaata tggaaagcta tttcccattt
     1681 ttcctctcta atgctcaggt caaagcgcta ctttcacgac ctctatatta tcatggttta
     1741 ttgactaccg gtatccagat ttctcccata tatttcccgt tggtaactcc catttcgact
     1801 acagcatttg gacccccaac ataggcgtaa tcttctactt ctcttaaaac atgcccaaag
     1861 gccgtattcg tcaaatcatt tcttaattct tcaggcgatt ctgctaaagc gattacaaca
     1921 agatacgttc cttttgggaa tgggatgatt cgggtagtgt ttggaacatc ctgactcgtt
     1981 tgaacaccga catagtacat cattttcccg ttatagggtt cgttgactgt aaagaaataa
     2041 tgattttcag cagcttgttt caatgcatct attgttccat taccgataac tttttgctca
     2101 aatgcttgtt tctctgcagc caaaccgaca aaatccgtat tgctactctt caattccaca
     2161 ccaatacccg tcaccgtaaa atctggtttt tcttcgatcc tatactttgc catttctccc
     2221 accctttcta ttatattaag ttcatcttac ataaaattaa agggaaatga cacgattacg
     2281 ctcttttttc tagggatatt tttatttttt aactattttt ttgctttcac tgcaatccaa
     2341 atctcactgt aaacattact gaaatcctct ccccattttg taaaagagat ctcgggagct
     2401 tcgactaact cataatctga agaaggaagc cattctgcat agattttccc ccaagtatct
     2461 tgtaaagttt ggggaaatgg ccctttatta ggaaaaatgg cccaagtgtg ttcttctata
     2521 gcaagcattt ctaaatcagc gtacggattt tcttttgtcg tagcaaagcc aatcatgtgc
     2581 atcaaactgc ccttttcttc caagcgggct ccatcaaatc cataagaaac gttgattact
     2641 tgatttggat ataaatctcc taacttatgc atctcttccc tttgagtctc cgtgatcgtc
     2701 tgcgctaact tttgtatttc ttgattgact ccttcaaatt tgacaggaac ttttttcgcc
     2761 acacctgcca cataaaactg ttctttcttt tcgatacgaa attccatcga ttttcctcct
     2821 ctgacatcaa taaaaaatgt caagcgtgga aaagattttt gtatgccgta tctgctgatt
     2881 tctgaaggca gatatcctga ccaatcacga aaagctcttg aaaaaccttc gactgtttca
     2941 tatccatatc taaacgccgt ttctgtcact cttgatccat ctaggagatc gcgattagcc
     3001 aaagccaatc tacggttttt gatatagtcc attagcgaca tccctgcaat aaacgtaaag
     3061 atgcgtttta attgatattc agagattcct gtattttcgg ctattatttt ataggaaagt
     3121 ccttctgtca aatgttcttc gatgtaatcg atcaattggt tcagatcttt aagcatattt
     3181 atcttcctcc ttgcttgcct ttttatcata ttatcaaaga aagtcaatta cttgatgaat
     3241 caggaaccaa tttagtcaga tgtagaaaat gttgtatgga tctaaagaaa attctcaagc
     3301 agcgtttcgt aacggataat tggtattatc tgcctatcta tgttttacct ttagaaaaat
     3361 aaattttggg gaagacacat tctaagtaaa caaagcggta aaaatatctt ttattccctt
     3421 agttaatccg cttactacct actgtacaaa gcaaattgtt gttcaaactg ctgatcagaa
     3481 cgctgcttgg atttttcttt gcgatacatc atacctcttt tatcaattgg aacaattatt
     3541 tatttctaac aaaatcactt catttttgtc agataaataa aaaacaaaaa aagttccatc
     3601 tttaaaacac gctattcttt tactttttct cacattctct aagaaaagaa aaggagaatg
     3661 aatttttgac aattcgagca aaagtgatta tcaaatatgt aaaatcagct acttttttct
     3721 taaacgtttc tttatactaa atataaataa gtgtttcatg aaaggagaac tagatgaata
     3781 attggcttga gtattttcct gaaaacgttt tggaacgcgg ttacagctat catctgcacg
     3841 gttttgtccg tcatttgaac tatacaagca agtacttgtc cgcaacagtt tctggcacgg
     3901 aagattataa ggtagtgatc acttgggatg aaaagaccaa catgacttgt gattgtctat
     3961 atgccataga aggaaaaaaa tgcaaacaca tggctgctgt cttattcgct tacgaagaac
     4021 gtccaataaa aaaatccaac tactcacttt ccgaactctc gtcccttgtt tcctccgcta
     4081 gttcctctct tgtacgggag cttttaacag agattcttat agaacatccg caatttatcg
     4141 aacgcttcaa agttaagatg ccatttcatg caataaatta ttcagataaa ttaacaacta
     4201 tcatccataa atacgatcat attatcaaga aaaacaaaaa caggaaaaca gcgaagttca
     4261 tcatggagat gcgaaagttc atccaagaag cagttgaatc gctcattcag caaaatgcct
     4321 atcttcccgc attcgaattg atcaatgaag tgattgctac tctcgaaaca ttctattggg
     4381 agcctgagga tgaacgaacc cttcttctga tagaggactg ttattattta tggaaagaat
     4441 tattagcaga agcacctcat gcagaaaaaa ggcaaatgtt ctcttggttc gtctgccaag
     4501 tcgatcatac tgacgcctct tattcaaaac gctactccat aaaaatcctg aaagaagact
     4561 ttcgggaaaa agaattttct aaccaaaaga aaaaaataga taaaaaaact aaagaaacgg
     4621 taaagaaaga cgatttcaat gaaaaatagc aagtaaacga taacaaccat cctaaatcca
     4681 caagaaatac agcagacctt gctttaaaaa cacgaatcat gcaaaggatg aagtaaagaa
     4741 gacttaacgc gtacccaata ttagtcattt gactattagg tacgcgtttt gttttataag
     4801 tcatgacaaa tttccttcgt tgctgggtag attttccggc attgttgata gattttatga
     4861 tacttttcta cattttgtaa gagaggttcc actgcatctg tatattggat aaaattctga
     4921 caacaacttt caggtgtgtc gaaccactct aatctttaaa gataaatatt aaaataacga
     4981 ttaataatat taacagtctt tatttttcac caccaaaaac ctatctttgc atatactaaa
     5041 tagacgtata tacacagata ggtttgtctt gtgaaaaata atgattatcc ttaccaaaaa
     5101 atgtttgttt ttctacatgt tcgtacttat caaaaaatat gatccttatt gaaattactg
     5161 gctatttata ttccaacgac ttcttgtgtt aatttttgtt atctcatagt aaattatgtt
     5221 tcattttcac ttaatttagt tgtcacgtgt tcgtcaattc gtaaaaacgg taaatatata
     5281 aacacacttg cggctataat taatatttgt aaaacagctg ctcgccagtc tccggctgtt
     5341 gctaaaaatc ccgatataac tggtggcgta gtccaaggaa tttgcactac tacatgatta
     5401 attaatccca cagcagttgc aaaatatgct actgtcaaag aaaaaatcgg tgcaagcaca
     5461 aagggaataa tcagactgat attaaataca ataggcaatc caaaaataat aggttcgctt
     5521 atattgaata tagcgggagt taccgataat ttagcaaagt ccttataatc ttttctacga
     5581 ctgaagataa atattgcaat caataaagca atcgtatttc ccgatccacc cattaccgca
     5641 aaggtatctt taaaggccat attgataata tgtggaattt gctctttgtt tgcataagct
     5701 gccatatttt cttgcatatt ctgaatcaaa aacggatcta acaatgaacc tgatattact
     5761 gcttgatgga taccaaatcc aaaaaataga ttagcaatag atgtaattaa caaaaatcct
     5821 ggtaaaccag ttgtaatttt acttaatggc tctgtaacta tcgtggtaat tagcttattt
     5881 atatccatat taaacaattg aatgattata aatgacaata aagcaaacga ggtaatcgta
     5941 ataatgattg ggattaatac attaaaagac tttaaaacag cgggaggaat atttccggat
     6001 agctgaatct gtagtttctt attattagaa agtttaataa ataattctgt agccgctaat
     6061 ccaacaaata tacctacaaa cattcctgaa gctcctgtat aggatatagg tatcacaccc
     6121 gtcacttcaa cagctttatc cgcattttca ggagtaaaca tcatctgcat aggtgttaat
     6181 acaacaaatg tcgaaatagc aactaatacc gctgcaatat gatttgtata atttctgctt
     6241 gcacacatat gataagatac tgctgctaca actaataatg tagttatgct taatgtacca
     6301 ttggcaattg aagtaccaat ttgttgtaac gtggtcaatg tactagtatc gacaatttcc
     6361 cccatgaatc ctgttggttc taagatcact gaattaataa gtgtcacaaa tcctgctaac
     6421 atcataaatg gcataaatgt agcaaacgca tctcgcaaag ttcttaaatg gatctgattc
     6481 cccaacttag tcgcaactat accaaatttt tcaaatacaa tgtccttcct gctacttttg
     6541 cccatatttt ttctccttta tgcttattgt aatcgattac tttttccacc caaataatac
     6601 atcacttatc aaaaaaagta aatactttta attaaatata tatatctttt ttagtataaa
     6661 aaaataagct ctgaaaaaat aattttcccg agcttatttt tcaacaaaaa cagcttcaaa
     6721 aacaaagtct tgatataaat gtctcgatac cgaatactcg aaaggtttac cagacgatag
     6781 agcaacaact ttgtctatct caatcataaa ttcacctgga tctattttta atatttttgc
     6841 gtcaaattca ttcgcagtat ctcctttgat acgtatgtgc gaagtcttta tttccagacc
     6901 cagttcattt gtgatataag catatacaga tttctttaaa tgttttggtt ccaagcctgg
     6961 tatgactgct aatggcatga atgtttgctc aatcgagtat ggttgacaat caataaaacg
     7021 gcttcgtaca atattatata catagtcttt tacactgata cttaatttat cagctacttt
     7081 tttcgatgga agttcaattg agaaagtatg gatttctgaa tctacgtgtt cttcaccaac
     7141 cacgctaaac aaaccttcgt ttggaccaag aactcgtgag ttatcaattg gtgttctgac
     7201 gacttcagtc ccatacccac ttctacttcg caaaactcct tcggaaacaa gtaagtctac
     7261 gcctttcttt actgttaact tgcttgtatg ataaataact gccaattcat ctcctgtagg
     7321 aagtttctca ccaattttat actctccatt tacaattttt ttctttatat ctaagtaaat
     7381 ttccatatac ttcgcaattt ttttcaatgg tgaccctcca atcaaaaaca tatcttcatc
     7441 tatactttaa cataaataaa ccgataaaat ccacatcaaa attatacaca caataaaaaa
     7501 gatatatata tttaaacaaa actatttact ttaagttgta agcggtgtta tcattaagtg
     7561 gaaacaggag gtaaaaaaat tatgacaact atgaaaaagc taacttttcc agatgatttt
     7621 tggtggggtt ccgcttggtc agctgagcaa gcagaaggaa aaggagaaac aggaaaagca
     7681 gagactatct gggatcgctg gtttaaagaa caaccttacc ggttttatca acgaatcgat
     7741 tcctctatta caaccgatca catccatcaa tataaagaag atatagaact aatgaaaaaa
     7801 actgggcata actcctttcg cgtttctatt tcttgggcac gaatgtttcc aaatggcggt
     7861 accggaaaag taaatcccaa agccatttct ttctatcgaa aattattttc cgaaatgaat
     7921 aataacggaa tcaaagtatt tgccaactta tatcattttg acatgcctgc tactcttcaa
     7981 gataaaggag gatgggaatc acgtgaagta gttgatgctt atgttcatta cgctgaaact
     8041 tgttttaaag aatttggtga cttagtttgt cattggttta ccttcaatga accacttggg
     8101 cctattttag gtacttatct tgaagatttt cattatccaa acctcattga ctttaaacgc
     8161 ggagcgcaag cagcattctt cactatactt gcacacgcaa aagcaataca agtatttaaa
     8221 gaactagctc ttcctagtaa aataggcgtt attctaaatt tgagtcctac ttttcctcgt
     8281 agtcaaaatc ctgctgatgt gaaagcagca gaaatcgctg atttatttta tacgcggagc
     8341 tttttagatc ctatggttaa agggaatttt tctaaaaagc ttatcctgtt attacaagaa
     8401 tataatcagc ttcctgaaga ttatacgaaa gaagatcttg tatgtatcta cgaaaatacc
     8461 gctcaaattc ttggactaaa ctattacgag ccacgtcgcg tcaaatctcg ttcttctatg
     8521 gtggatacat ctagtccatt tttaccagag tggtttttcg aaccacatat catgcctgga
     8581 cgacgaatga acgaataccg gggatgggaa atttacgaaa aaggaattta tgatttgtgc
     8641 atggatatca agaaccatta tggaaacatc gaagcattta tctcggagaa cggaatgggc
     8701 gttgcggatg aagaacgttt catggatgat acagggcaaa taattgatga ttatcggatt
     8761 gagtatatca aagatcatct tgcttactta tggaaggcaa taaatgaagg atgtaacatt
     8821 aaggggtatc atctttggac atttattgat tgctggtcat ggatcaacgc ctataaaaac
     8881 cgatatgggc tggtttcact cgaccttact actcaaaaaa gaacaattaa aaaaagtggc
     8941 gaattttaca agcaattaag tgatacaaat ggatttagat acgatgtaga cttgttagtt
     9001 gaataatatt ctaaaattcc acttgaagtg aacacattag aaaacaagaa taatgagaaa
     9061 taccaataac aaatactttt tcttcatact tcatttatac ttgtcagtat aagagactgt
     9121 gacaaatgta gtgatcctca gaagttaggt ctggaaatct aacttgagaa gattttttta
     9181 aactacgtcc agtctttttt atttgtttaa aattgaagtt gacaagtctt cattaaaaat
     9241 ttagaatatt cacaagtttg acgacaatta tgaaaattta tgtcgtgcta aaggagtttt
     9301 gttattggat gaaaagttag atataaaaac tgcgatcaaa gacacgttac ctaccgtttt
     9361 tggttatatc ggtattggac ttgcatttgg tatcgttggg aaagctgccg ggtttcatcc
     9421 gctggtcgtc acgttgatgt ccctgctgat ctatgctggt tctgcccaat ttatcacggt
     9481 cagcatgctt gctagccata gtccactgct ctccatcgtt ctctcgacct ttctagtcaa
     9541 ttcccgaatg attctgatga gtatgacgat tgctccttat ttcaagaaaa atcggctgct
     9601 tcaaaatctg ctgattggca cattgctgac agatgaaagt ttcgcactgg gaatgaataa
     9661 attgaactat actggacaaa agttaaactt tcgttggatg aatacagcta attggatttc
     9721 ttatttgacg tgggtgggtt cttctcttgt tggcgcactt ttagggaatt ttattactga
     9781 tccgaaaaag tttggtttgg atttcgccat cgtcgctatg tttatcgggt tactttatct
     9841 tcaagtcatt tctgatagaa acacctctaa acgattacag ttgatcctga ttggtctcac
     9901 tttgattctg gtatatgtcg gactgatttt tatcccaagt aacttagtga ttttagttgt
     9961 aacactgatt gggtgcggat tgggggtgtg gatcaaacat gccttctttt gagtttacat
    10021 tgctgactat tttaggttgt actttagcaa cttggctttc taggatccta ccatttgtct
    10081 tactgaaaaa attcgatttg ccgcaaccgt tgcttgagta tttaagtttt gtcccgatcg
    10141 tcattatgtc tgctttatgg ttcagcagtc tatttaccca aaatatcgga catctgccgc
    10201 agatcaatct cgaaaatgct ttggcttcag ttcctacact tttagctgct attctttcaa
    10261 aaagcttatt ggtgatcgtc ttggtaggaa ttctttccct ttcgttgatt cgcttattat
    10321 aaataaaaaa acaagagctt atggcaaatt actgtcatgc gctcttgttt ttatcaaagt
    10381 caaataagaa aaaaatcggc gtattcttgc ttatccgttg aacggaccac taacaacgac
    10441 ttttcctaat atatgtcctg tttctacgtc tttggttgct tgtttcaagt tcttcgcatt
    10501 catcccatct gaatagacct tgctcaatgt actgcggata atcccttcat ttgctaaagc
    10561 agcaatgtgc gctaaagctt ctccttgcgt ttcgatttca tagttatagt ctgttttagc
    10621 gaacatatac tcccagtcga agctgataga ttggtttttc caaacaccta tatctaacgg
    10681 ctctttgatc cctacgatcg ttcccacatg tccaaatggt cggacaaggt gtttgatttg
    10741 atcaaagtag cgggtaatat cgaataatac agcgacatac tctacttgat cgattcctaa
    10801 ttcacgaaaa cttttactta agtcgtgatg ataatcgatt ggatagtcta cacctgtttt
    10861 tttcagccat tcaaaattct tcggacttgc agtcgcgtag acttctaggc cagcccaatg
    10921 cgctagttga ttcaaaatcg aaccgacgcc cccagctccg ttgatcacta agattttttt
    10981 cccgcggttg gcattttctt ctggagtcag accaaatttt tcaaataaca attcataagc
    11041 ggtcaatgag gtcaacggca aggctgcggc ttcttcatct gaaagatttt ttggtgcaag
    11101 agcgacaata cgttcgtcaa ccagttggta ttcttgattg cttcctgctc ttgtcgttgt
    11161 acctgcgtaa aaaacgcgat cgccgacaga aaatttttgc acttcatcac ctacagcaac
    11221 aactttcccg actccatcaa accctaagat acgtaaagca tttcttatcc cgttatcttg
    11281 gcgaagtttc gtatcgacag gattaacaga tactgcgttg acttttacca gaagatcccg
    11341 tgaactgggt acaggtatct gttctttttc atcaataaag ctttttggat catcgatcga
    11401 caacccttca taaaaaccga ctaccttcat taattccttt tctgacatac gaattcctcc
    11461 ttagcttcat caagttggag catccataca gttttcattt caatatatat agtatgctat
    11521 ttgttccact tttattcatc ataattattc tcttacttaa agtaaactat ttgcactttt
    11581 ttggttaaaa aagaggttgc gacaagactt ttgtcacaac ctcttttaaa cggtgttcaa
    11641 tcaactgacc ctcggtattc gctcaaaaaa cgacaaa
//
LOCUS       DBNICI010000093        11471 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.093, whole genome shotgun sequence.
ACCESSION   DBNICI010000093 DBNICI010000000
VERSION     DBNICI010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.093"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..731
                     /locus_tag="AC1ZCC_001920"
     CDS             <1..731
                     /locus_tag="AC1ZCC_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIQ1630165.1"
                     /translation="SILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNV
                     ALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSY
                     GGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINK
                     GEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLL
                     GSIQTNLPESVLLDCGMDFLKDIC"
     gene            789..2828
                     /locus_tag="AC1ZCC_001921"
     CDS             789..2828
                     /locus_tag="AC1ZCC_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIQ1630166.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2937..4088)
                     /locus_tag="AC1ZCC_001922"
     CDS             complement(2937..4088)
                     /locus_tag="AC1ZCC_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIQ1630167.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4242..4772)
                     /locus_tag="AC1ZCC_001923"
     CDS             complement(4242..4772)
                     /locus_tag="AC1ZCC_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009268 - response to pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HIQ1630168.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4871..5221)
                     /locus_tag="AC1ZCC_001924"
     CDS             complement(4871..5221)
                     /locus_tag="AC1ZCC_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HIQ1630169.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5378..6907)
                     /gene="ahpF"
                     /locus_tag="AC1ZCC_001925"
     CDS             complement(5378..6907)
                     /gene="ahpF"
                     /locus_tag="AC1ZCC_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HIQ1630170.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6917..7480)
                     /gene="ahpC"
                     /locus_tag="AC1ZCC_001926"
     CDS             complement(6917..7480)
                     /gene="ahpC"
                     /locus_tag="AC1ZCC_001926"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HIQ1630171.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7624..8286)
                     /locus_tag="AC1ZCC_001927"
     CDS             complement(7624..8286)
                     /locus_tag="AC1ZCC_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIQ1630172.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8470..10116
                     /locus_tag="AC1ZCC_001928"
     CDS             8470..10116
                     /locus_tag="AC1ZCC_001928"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HIQ1630173.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10107..11189
                     /gene="uxuA"
                     /locus_tag="AC1ZCC_001929"
     CDS             10107..11189
                     /gene="uxuA"
                     /locus_tag="AC1ZCC_001929"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HIQ1630174.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11297..>11471)
                     /locus_tag="AC1ZCC_001930"
     CDS             complement(11297..>11471)
                     /locus_tag="AC1ZCC_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HIQ1630175.1"
                     /translation="DCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEA
                     LMDQSKKENQVTS"
BASE COUNT         3842 a         2142 c         2160 g         3327 t
ORIGIN      
        1 tatctatttt gttactgctt acattagtag ttggctattt ttcgattgag tttatccatg
       61 gattttcgtc tgcaaaacag agttcaactg tgaaaaaggt agagccgaaa agtatcccta
      121 ccacactaaa tgtggctttg attggttcag atgctcgttc gaaagacgaa aacggtcgct
      181 cggattcact tctggttgca caatacaacc agaaaacaca acaagcaaaa ctgatctcta
      241 tcatgagaga ttcatatgtc gatattccag gttacggaat gaataagata aatgcagctt
      301 actcttatgg gggagttgat ttattaaatc aaacattaaa ggaaaatttc aaatttgaag
      361 ctccctatta tgcaagtatc acatttcaag attttatcga ttgcgttaat gaactgtttc
      421 ctggtggagt caagatcgat gcagaaaaat ctctagattt agatggcgta tatataaaca
      481 aaggagagca agtgatggat ggtaatactt tactccagta tgctcgattt cgggaagaca
      541 aagaaggaga cttcgggagg atcagaaggc agcagcaagt gatcaaagct atctctcagc
      601 agctgaaaga cgtaacttca atattcaagc tacccaaagc ggttgggaaa ttacttggta
      661 gtatccaaac gaaccttcca gaaagtgtgc ttctggattg tggtatggat ttcctcaaag
      721 acatatgtta gaataaacag gtataaatag tgaaataaag gaatgacaag caagagaagg
      781 aggaaaaaat gaaaagaagt gacaagcacg gcaaaaatcg aacaggcgct tatattgccg
      841 gcgcagtgat cttaatagca gctgcgggtg gcggttattt ttactaccag cactatcaag
      901 aaacccaagc agtagaagct ggagaaaaga cagttgagca atttgtccaa gctttgaaca
      961 agggagatta taacaaagct gcagaaatga catcaaaaaa ggcggcaaat aaaagtgcat
     1021 tatctgaaaa agagatctta gataaatacc agaatatata cggtgctgct gatgtcaaag
     1081 gacttcagat atcgaatcta aaagtagata aaaaagacga ttctacttac agtttttcat
     1141 ataaagcaaa gatgaatacc tcattaggtg aattgaaaga tctttcttat aaaggaacat
     1201 tagacagaaa tgatggtcaa accacgatca actggcaacc caacttggtt tttccagaaa
     1261 tggaaggaaa tgacaaagta agcctgacca cacaagaagc agcaagaggg aatattatag
     1321 atcgaaatgg tgaaccatta gcaacaacag gcaaactaaa acaattagga gtcgttccaa
     1381 gtaaacttgg agatggaggc gaaaaaacag ccaatatcaa agcgattgct tcctccttcg
     1441 acttaacaga agatgctatc aatcaggcga tttcacaaag ctgggtacaa cccgattact
     1501 ttgtcccatt gaaaatcatt gatggagcaa cgccagaact tccagctgga gctaccattc
     1561 aagaagtaga cggcagatat tatcctttgg gtgaagcagc tgctcagctg attggttacg
     1621 tgggagatat cacagcagaa gatattgata agaatccaga attaagcagt aatggaaaaa
     1681 tcggtcgctc aggtttggag atggcttttg ataaggatct tcgtgggaca acaggtggga
     1741 aattaagcat tacagatgca gacggtgtag agaaaaaagt tctgatcgaa catgaagttc
     1801 aaaatggcaa agatatcaaa ttgacaatcg atgcgaaggc acaaaaaaca gcttttgaca
     1861 gtctaggagg aaaagctgga tcaactgttg cgacaacgcc aaaaaccggt gatcttcttg
     1921 cgcttgctag ctctccaagc tatgatccaa acaaaatgac aaacgggatc tcacaagaag
     1981 attacaaagc ttatgaagaa aatcctgaac atccattcat cagccgattt gcgacaggtt
     2041 atgctcctgg atctacgttt aaaataatta cagcagcaat cggtctcgac aacggcacta
     2101 tcgatccaaa tgaagtgttg acgatcaacg ggcttaaatg gcaaaaagac agttcttggg
     2161 gatcttttca aacaacgcgt gtcagtagtg atgtatcaca agtagactta aaaactgctt
     2221 tgatctattc cgataatata tatgcggcac aagaaacgtt gaaaatgggt gagaaaaaat
     2281 ttcgtacagg cttagataaa ttcatttttg gtgaagacct tgatttgcca attagtatga
     2341 atccagcaca aatttctaat gaagatagct ttaactcaga tatcttgcta gctgatactg
     2401 gatatggaca gggcgaactt ctaattaatc ctatccagca agcagcaatg tattctgttt
     2461 ttaccaacaa tggcacactt gtctatccta aattgattgc agataaagag acaaaagata
     2521 agaagaatgt catcagcgaa acagcagtac aaacgatcgt gccagatctg agagaagttg
     2581 tgcaagatgt aaatggtaca gcacattctc tttctgcttt agggattccg ttggcagcga
     2641 aaactggtac agcggaaatc aaagaaaaac aggatgtaaa ggggcaagag aacagtttct
     2701 tgtttgcttt caatcttgat aaccaaggtt atatgatggt cagcatgttg gaaaataaag
     2761 aagatgatga ttcagcaacc aaacgagcat cggaactatt gcaatacctc aaccaaaatt
     2821 atcaataaaa aattgctttt taagtacagt aaaaggactg tggcgttatc gtcacagtcc
     2881 ttttttcaaa taaacggttt tcacgatgtc tctttcacaa cttctttttg atttactcac
     2941 acttcataaa ctttttttgt aaaatatttg agaacaagtg gagaaataag tgtacttaat
     3001 aaaatcacga taactaaagg tgaatagtac tgtggttcga tcaattgact ttgctgtcca
     3061 atttgtaaaa tgatcaaagc catttctcca cgcgaaatca taccagcacc gaccatcaat
     3121 gaactatgtt gagaaaaacc ggcaatctga gcaccaaaat aacctccaag caatttggtc
     3181 aaaatcgcta caagcgtcaa tatagaaata aagagtagtt gttcactaaa gtcagaaaaa
     3241 tcaacttcta atcctacact gacaaaaaat actggtataa atactgcata tcctaaagcc
     3301 tctacattgt ggtatacttc ttctttaacc tttgtctgac tgacagctat cccagcaaaa
     3361 aaagcaccaa tgacagagct aagcccaaca agatccgcta cgtaagacat tcctaaacaa
     3421 ataactagcg atacgatgat aatagaagaa tttgcgtaca ctttttctgc aaatgaaagt
     3481 aggaaaggcg caatccattt gactaataag aaaataaaaa caaaataaat tagctgttca
     3541 atcaaaacaa gcggtaaaga agcattgcta gaagcttccc ccgttagaaa agataggctg
     3601 aaacttagaa ctaaaacgac taaaatgtca tctaccacag aagcacccaa aattgttgat
     3661 ccttcttttg tattcactac gtttagttct tttaatactt ctactgaaat actgacagaa
     3721 gttgccgcaa gaataatccc aaagaaaatc gcttcatttc ccgaaacttg gaatgctttg
     3781 cctgtcaacc agccaaaaac aaccgggaag atgatcccaa gcaaagcaac gaacatacca
     3841 ggtcggaaat attttttcaa aagagataaa tcactttcca gaccagctaa aaacattaat
     3901 aagatcacac caatctccga aaagtcatgg actagaatat caggtttgac ccaattcaat
     3961 ccagcttggc ctagcaaaac accaacaagt aattgtccaa tcactgctgg gatacctaat
     4021 ctccgcgaga tatgtgagcc aatcgttgtt gaaatcaata tcaggcataa aataccaata
     4081 aattccattc tcatcattcc tttgtataaa atatagcccg aacttagcat aacatacatt
     4141 attgaaaaaa taggaaaata tactcacaaa aaaaccaaca atccaaaaga tttttttgga
     4201 ttgttggttt ttatacggtc tattttaata actaaaaatg attaacgtat agtcattttt
     4261 cttattctga tgaaattgat aatctcctgg atgcccatga aaatcaaaat aaagccgaat
     4321 atttggaata gaagcatcaa acttttgaat ggattgaaca ataagacgaa tccgccaatc
     4381 aacaacagaa tactactata aacactccaa cttttagctt ttgtattcat gctgaagaac
     4441 aactggaaca agctgttgat gataacagct aatccaagaa gaatcggcag gatagaaacg
     4501 attgcaggtg caaaagcata gacgatcaga gctaagatca ggaaaaaaag accactgaac
     4561 agaccgatac tccaagaacc tgttttcctt ttaaaacggt aagcctcaaa aagattcaac
     4621 aacccaagca aaacaagata ggcggtgatc agataaccga taaaatgaaa aacagcagaa
     4681 gggtttagca caatcgcaat cccagaaata atataaacag cagctcttaa taaggcgtat
     4741 ttttgaatat tttgtttaat cgtctcaaac atataagaaa acctcctttg acttatttta
     4801 acgtgaacta gtcattttac caaattaaat gttacaaaat gagcgaagga gaagaatatc
     4861 tctggaaaaa tcaacttctg aggatctttt ccattttttt gccttttgct agctcatcga
     4921 tcattttatc taaataacga atttctttca tcaagttatc ctcgatgtct tctactcgta
     4981 caccacaaac aacacctgta atcaaggaac gtgaaggatt ggggacaggg gcttccgcaa
     5041 aaaattgttc gaaagtcact tgtttttgta gttgatattc tagttctgtt tgggaataac
     5101 ccgtcagcca acaaatgatt tcatctactt cctcttttat acgatctttt ctctccgctt
     5161 ttgctacata cataggataa acgcttgcaa aacttgtcgt aaaaatacga ttttgcgcca
     5221 taatcagtca cctttctttt tttcaaatta tttactactt atagtatata agaaaaagag
     5281 aagaaaaagc gattattcaa aaaaacaaaa aggattgtga caaaattctt gtcaccatcc
     5341 ttttttccgc tctctgactt tttacaatct tcatagctca agtgcgcatc agataatcga
     5401 atgctcctaa agctgcagta gctcctgaac ccattgaaac aatgatttgt ttgtatggac
     5461 tatctgtaca atcaccagct gcgaaaacgc cggggatatt cgttgcgcca tttttctcgg
     5521 tcactatttc atttcttgga ttcatttcga tcgttccatc tagccaatcc gtattaggga
     5581 gtaagccgac tagaatgaat actcctgaaa cagctagtgt atgcatctcc atcgtacggc
     5641 gatcgatata ggagagagat tctacctgat tcgtaccgct gatttctttc gtcgctgcat
     5701 gtttgataac agtgacattc gcaagagaga ctagtttatc ctgtaagatt tgatctgctt
     5761 ttaattctgg taagaattct agaacatata catgttttgc taacccagcc aaatcgattg
     5821 ctgcttcaac accagaatta ccaccaccga taacagcaat ctcttttcct ttaaataaag
     5881 ggccgtcaca gtgtgggcaa taagcaattc ctttattttt aaactctttt tcgcctggta
     5941 cattgataga acgccagtgc gcacctgtcg cgatgatggc ggtttttgct ttaagtgata
     6001 taccgttagc taagccaata ttaataaaat gttgctcttt atttaaggaa acggcctgtt
     6061 gattcatcat aatgtcgata gggtaactac gcacatgact ttctacttgc tgcatcaatt
     6121 ttggaccttc tgtgtaagag gtaccaatca tattttcaat ccctagtgtt tcaacgactt
     6181 gtccgccgaa agaatcagcg acaagtcctg tacggattcc tttacgtgct gcatagatag
     6241 ctgcactact agctgctggt ccaccgccaa ttactaatac atcataaggt tctttttcaa
     6301 acaactcttc ttcaatcaaa gggccagtga ttttttcaac aatttgttcg atcgtcatgc
     6361 gtccgctact aaattcttct ccatttaaaa acacagttgg cactgccatg atttttttat
     6421 catcgatttc tttttggaac atcccacctt caatcatcgt atgagtaata tttggattta
     6481 atacagctaa tatattcaat gcctgtacaa cgtcggggca attatgacaa gtcaagctaa
     6541 cataactgtc aaaatgcaat gtttgtgtta tttttttgat tttaagactc aaagattcac
     6601 tgattttcgg tgcccgtcca ctaatttgta acatagccag taaaaaagag gaaaattcat
     6661 gaccgagggg gataccagca aaaacgattc cactttcttt gccaactgga ttgatagaaa
     6721 aactaggtgt gcgcatcgaa tctctttttt cccaagagat tttaggagac atttctgcta
     6781 tttccttcgt aaattcagct agtttttgac tattttcact attatccaaa ctaacagaaa
     6841 aaacaatagg agattcaatc agtgctaaat attgttcgag attttgttta gtttctttgt
     6901 ctaacatatc caccacctaa atctttccaa caagatcaaa acttggtttc aatgtttcgc
     6961 cactttcttt ccattttgcc ggacaaactt cacctgggtg ggtacgtaca tattgagcag
     7021 cgcgaatctt atcaatcagt gcactagcgt cacgaccgat tccatctgca ttgatttcca
     7081 tagcttgaac gattccgtct ggatcaataa taaatgttcc tcgctgagct aatccttgct
     7141 cttcatccaa aacatcaaaa atacgagaaa tctgatgaga cggatcaccg atcatcacgt
     7201 attcgatttt accgatagca tctgatgtat catgccatgc tttgtgagta aagtgggtat
     7261 cggtagagca agaaaagact tcaacaccta aagatttcaa tgtactgtat tgttcttgta
     7321 agtcttctaa ttcagtagga caaacaaaag taaaatcagc tggatagaag caaagaatac
     7381 tccagttccc cataagatct ttttcactga cttctaaaaa ttctccttga tgataagcat
     7441 gtgcattaaa ttcttcgatt tttttcccaa tcaatgacat agtaaaacct ccaataataa
     7501 ttatttattt ataattattg taatttagtt ggagttgcaa agtcaatgga tattttgtta
     7561 tttaataaag attactcaaa aacgaaggtt aattataaaa atagaagagg tcgcaagaaa
     7621 atgctactgc tggaaatact cgggataacg ttgagtaaca gtttcctgtt ctttcagcat
     7681 catgtgtaag tgcaatcccg tttgaaatcg agcttcttct ggttttttat caactaaagc
     7741 cttgagtaaa tcataatgtt gttccatgat cgtttcccat ggaagcccct gaatggtcac
     7801 tcgtaaccag cggaaacgtt ctaaatgggt attatggtca tctaaccagt tccaaacttc
     7861 ttttcttcct gcaatttcga aacaagcttc atgaaataaa ttatctgcac ggaaaaaagc
     7921 gcgttcgtca tttattttga ctgctttttc ttgttcttct aaaatcgttt ctagcactat
     7981 tcgtgtttgt gtgtccaatt tggaacaaca ttcaatcatg atttgctctt ccaactgttc
     8041 tcggacaaag cgagcatttt ctgcagaaac aagattaatt ttggaaacat acgtaccact
     8101 ttgaggtatc gtatacacga gttcttgctg acgtagttga atcaatgctt cgcgaatggg
     8161 agtccggccg atagcaagat gttcctccag tgccttttca gatattttcc ttccaggttc
     8221 taagtcagca tagataattt gtttacgaat cgcttgataa gcttgtgatt gcaagctgct
     8281 tttcatcttt attcctccaa aatcatttct atgaacgtag tataacagaa gaggtctgaa
     8341 aaacaatctg gtatgtcaca ttaactagta aatttgtata aatagtaaaa ttttggttga
     8401 catagcgatt tttattgtat aaaattcact tgaatacaaa aactaatatg ttagtttagg
     8461 aggggaaaaa tgattgattt aaacaactgg aggaacttta tccctgaatt atctcaaaaa
     8521 gaagtagaaa tacctcattt tgaccgtgca tcattaagag aaaatggaca aaaaaatcct
     8581 gtctggatcc atttcggtgg cggaaatctt tatcgaggat ttcatgcaga aatcgctcaa
     8641 cgtttagcgg atctaggcga attgtccact ggtgtcattg tttgtgaaac attcgatgaa
     8701 gaaataattg aaagggctta ccaagatttt gatgatttga ttcttgaagt ggtcatgcca
     8761 agcagtggag agttcgtcaa acgtatatta gctgcgacag ctgacagtct gtattgtcat
     8821 ccagattgtt ctgaacactt tgataagatg attcgacact ttgaaaatcc tagtttacaa
     8881 ttagttactt ttgctattac tgaaaaaggt tatcgattga aagatacaaa taataaatat
     8941 ataggaccga ttgcagtcga tataaaaaat ggtccaaaga agccatcaca tacaatgagt
     9001 attcttgctg cgctacttta taaacgtttt ttatctggag cttggccgat tgcacttgtc
     9061 tctacagata atttttcaca aaacggtagt caattacgta cttctttgct aacgatcgca
     9121 aaagaatggc aaaaggaagg ttatgtacaa aatacattta tccaatactt atcgaatgaa
     9181 gaaattgttg cgtttccatg gacgatgatt gatcgtatca ccccgaatcc ttccttgtca
     9241 gttagcaaaa tgttggcaaa agagggcttt tcaaatatgt cgattttcca aaccgcaaaa
     9301 ggaaataata ttgctccatt tgtcaataca gaggacatcc attatttaat catggaagat
     9361 cattttccca atggacgtcc gaaattcgaa aaagctgggg tattcttgac tgatcgtgca
     9421 acggtagata aagcagatac gatgaaggtg acgacttgcc ttaatccttt gcatacgtct
     9481 ttagctatta tgggttgttt actggggtat aggagcatag ctgaggagat gaaagatcca
     9541 gatttatcgc tgctagtttc tgaaatcggg tacaatgaag gcctcccagt tgtggagtct
     9601 ccaggtatca ttgatccaaa gatctttctg gaagaagtaa ttactgtgcg cttttctaat
     9661 ccaaatattc ctgatatgcc tcagcgaatc gccacggata cctctcaaaa ggtggcgatt
     9721 cgttatggtg agacgattaa aaaatatatg gaaatagaag ttaaagaccc gcagacactt
     9781 gtattcatac cattgactct cgctgcttgg cttcgatacc ttattggcat tgatgatcaa
     9841 ggaaaaccat tcatcccgag tccagaccca ctattgacag actttcagca taagctttcc
     9901 gatattcaac tgggtacgca agatacatca catattcatg agaaaatggc tcctattttg
     9961 tcaaataaag aaatttttgg tgtagatctt taccaagcag agcttggcag aaaaatagaa
    10021 gaaatgttca tcaagatggt agcaggaccg aatgccgtac gaaaaacatt acaggattac
    10081 ttacaagaac atggaggaaa tttatcatgg aaatgacttt tagatggtac ggatcacagg
    10141 aagaaaagat taccttagat gaaatcagac agattcctgc ggtcaaaggg attgtaggca
    10201 cattgatgga tataccagta ggagaggtat ggcccaggga gaggatcaaa gaactaaaag
    10261 aagaaattga gtctcgtggg ttaacactaa aagtaattga aagcgtcaat gtgcatgatg
    10321 ccattaaaat tggattacct tcccgtgagc agtatatcga aaactataag cagacaatcc
    10381 gaaacttggc agaatatgga atagaagtca tctgctataa ttttatgcct gtatttgact
    10441 gggtgaaatc tgaccttgac taccgcttag aagacggctc atccacgtta gcttttatat
    10501 ctgatgatat tccagatgat ccaagagaaa tcgtcgcgcg catcgaacat tccagcaatg
    10561 aatttgaatt gccgggatgg gaacctgagc gcttagctca tatcaaatcc ttgcttgaag
    10621 cctatgcatc agtagacgaa gaaaaacttc gagaaaattt tgcctatttt ctccaatcaa
    10681 tcatcccaac atgtgaagag gtaggggtca aaatggctgt acatccagat gatccgccat
    10741 actcgatttt tggattacct cgaatcgtaa agaatcgaga agatttagat tggttgtgcc
    10801 gcatagtaga cagcccttat aacggaatca ctctttgtac agggagtatt gcagaagatc
    10861 cagaaaatga tgtatatggg attttagcag aattttgtca aagagatcga atcccatttg
    10921 cacatgttcg aaatattaaa tttttagcta atcgagattt ctatgaggca ccgcataaat
    10981 ccgaatacgg ttcattagac atgtaccgca tactaaaaac aatgtatgac aacggattta
    11041 acgaatacat ccgtcctgat catggaagaa tgatctggaa tgagacagga agaccaggat
    11101 acggcttata tgatcgagca ttaggtgctg tttatctaaa tggattatgg gaagcaatcg
    11161 aaaaaggaaa caaaagtaaa gataattagt gaaaaaaact gtaattttat tatgtaaata
    11221 agaaggatgt tcaaattctt gatacataaa tccggaggtt gttaataagc attcggattt
    11281 atgtatctta tttggtctag gaagtaactt gattttcttt tttcgattga tccatgagtg
    11341 cttcttcttt taattcaaag atatattctg agatcctcat aatcactttt ttccagtctt
    11401 ctggtgtgat gattcctttt aaaatataga tttttttgat atgagaaggc aattgtttca
    11461 aagggcagtc g
//
LOCUS       DBNICI010000094        11321 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.094, whole genome shotgun sequence.
ACCESSION   DBNICI010000094 DBNICI010000000
VERSION     DBNICI010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.094"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..1579
                     /gene="mdcD"
                     /locus_tag="AC1ZCC_001931"
     CDS             <1..1579
                     /gene="mdcD"
                     /locus_tag="AC1ZCC_001931"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HIQ1630176.1"
                     /translation="PSLPAQGIVPESDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGE
                     VSGAKIVAALEHVLEENKKGNEIFPVIVLDTGGVRLQEANYGLLSISEIGNVIIALKQ
                     YVPVIGLIPGRVGSFGGMSITSALMSYLIATKKARVGLNGPEVIEQEAGVREFDSSDK
                     DLIWNTLGSRQRVESKIIDELVTDSVEAIKAAVIAAMDERKDTHRSESSAFYLSLLDS
                     LDLSKPMAIDDYNEAIANHQAKSIDLPEVAEGNEPAAASVGYDWFAALTGMKNPTSSI
                     STVYYGKSSKFIEEAIVTSIVPDPKNPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDK
                     KVKRPIIVVIDVPSQAYGYNEELIGIHVALANSAAAYAKLRQAGHPVIGLIAGNAISG
                     AFLAHGLQSSRLIALNNKEITVQAMSKESAARVTKRSVAEIEAAAEKVPAIAYDINNY
                     TKLGAIYHFLEDITDQAASEQNVSKVIEAINEAIQDVRNTNDTMLTNRYTNETAKTFG
                     RVETNKVRAKMDEEWDA"
     gene            1583..2176
                     /locus_tag="AC1ZCC_001932"
     CDS             1583..2176
                     /locus_tag="AC1ZCC_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HIQ1630177.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            2200..3276
                     /locus_tag="AC1ZCC_001933"
     CDS             2200..3276
                     /locus_tag="AC1ZCC_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIQ1630178.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            3301..4170
                     /gene="mdcB"
                     /locus_tag="AC1ZCC_001934"
     CDS             3301..4170
                     /gene="mdcB"
                     /locus_tag="AC1ZCC_001934"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HIQ1630179.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            4281..5045
                     /locus_tag="AC1ZCC_001935"
     CDS             4281..5045
                     /locus_tag="AC1ZCC_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HIQ1630180.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            5534..7627
                     /locus_tag="AC1ZCC_001936"
     CDS             5534..7627
                     /locus_tag="AC1ZCC_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HIQ1630181.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            7652..7954
                     /locus_tag="AC1ZCC_001937"
     CDS             7652..7954
                     /locus_tag="AC1ZCC_001937"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIQ1630182.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            7981..9270
                     /locus_tag="AC1ZCC_001938"
     CDS             7981..9270
                     /locus_tag="AC1ZCC_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIQ1630183.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            9289..9696
                     /locus_tag="AC1ZCC_001939"
     CDS             9289..9696
                     /locus_tag="AC1ZCC_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIQ1630184.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            9719..10588
                     /locus_tag="AC1ZCC_001940"
     CDS             9719..10588
                     /locus_tag="AC1ZCC_001940"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0030388 - fructose 1,6-bisphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HIQ1630185.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            10590..11210
                     /locus_tag="AC1ZCC_001941"
     CDS             10590..11210
                     /locus_tag="AC1ZCC_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIQ1630186.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
BASE COUNT         3732 a         1978 c         2423 g         3188 t
ORIGIN      
        1 gccaagttta ccagcacaag gaatcgtccc tgaaagtgat gacgggatcg ttgtcagccg
       61 tggcacgatc aatggcaaac aagcagtagt aatcgctatg gaaggaaaat tccaaggtgg
      121 cggtatcggt gaagtttccg gtgcaaaaat cgttgctgca cttgaacatg tgttagaaga
      181 aaataaaaaa ggaaatgaga tctttccagt cattgtacta gatacaggtg gtgttcgttt
      241 gcaagaagca aactacggac tgttatctat ttcagaaatc ggaaatgtaa tcattgctct
      301 aaaacaatat gtaccagtca tcggtttgat tccaggacgt gtaggatcat ttggcggtat
      361 gtcgattact tcagcattaa tgagttattt aatcgcaaca aaaaaagcga gagtcggttt
      421 gaatggacca gaagttattg aacaagaagc aggcgttcga gaatttgatt ctagtgacaa
      481 agacttgatt tggaatacat taggatctcg tcaacgggta gaatcaaaaa tcattgatga
      541 attagtgact gatagtgtag aagcaatcaa agctgctgtc atcgcagcaa tggacgaacg
      601 aaaagacacg catcgcagtg aaagctcagc attttattta tcattacttg attctttaga
      661 tctttcaaaa ccaatggcca ttgacgatta caatgaagca attgctaacc atcaagcaaa
      721 atcaattgac ttaccagaag tagcagaagg aaatgaacca gctgcagcaa gtgttggtta
      781 cgattggttt gcggcactaa ctggcatgaa aaatccaact tcttctatta gtactgtgta
      841 ttatggtaaa tcaagtaaat tcatagaaga agccatcgtg acatcaatcg tacctgatcc
      901 taaaaacccg atgtaccgtg tacgaaacgg tgaagttggt ttagttgaag gtttccgcat
      961 gggacaaatt ttggatcacg tttacgaaga agataaagac aaaaaagtga aacggccaat
     1021 catcgttgtg atcgatgtac caagccaagc atacggctat aacgaagaat tgatcggtat
     1081 ccatgtggct ttggctaata gtgctgctgc ttatgcaaaa ctccgtcaag ctggacatcc
     1141 agttattgga ttgattgcag gaaatgctat ttcaggtgca tttttagcac atggattaca
     1201 atcaagccgc ttgattgctt taaacaataa agaaattacc gtccaagcga tgtctaaaga
     1261 aagtgctgca cgtgtgacaa aacgttcggt tgctgaaatc gaagcagctg ctgaaaaagt
     1321 tccagctatc gcttacgata tcaataatta tacaaaacta ggagcgatct atcatttcct
     1381 agaagatatc accgaccaag ctgcttcaga acaaaatgtc agcaaagtca tcgaagcaat
     1441 caatgaagcc attcaagatg tccgcaatac aaatgacacg atgctgacaa atcgatacac
     1501 aaacgaaaca gccaaaacat tcggacgtgt cgaaacaaat aaagtccgtg ccaaaatgga
     1561 cgaagaatgg gatgcataaa aaatgattca agcacatgat attgtccttt ttgataaaca
     1621 tgaaatagaa gggttcacct tgcctgaatg ggtcgttcta actgaaaaga tgttcggaac
     1681 agttcgtcgt gcgtcgacgg atcacgcaga aatactaccg attggtttac gtggacctgc
     1741 ccgcaatcag agatggtcaa cagaaatctc aaaacatgcc gtcaaacaag tcattcatcc
     1801 gtgggacatc atcgaacaag aatcgtatag acaagagaaa atcatggatt atcctgttta
     1861 tcagcaattt catgctgcta gaaacatatt atcaggatgt aaatggggaa tcggcggcag
     1921 cttaggattt gaattatcga ctgggattcc ggcagtaaaa gaaaccagtg attttgattt
     1981 gctgctgtac gcaaattcac cgattgaatt gccaattcag gtaattcaat cgcatcctgc
     2041 tttttttgag caattcgata cccaagtagt gactcggcaa ggcggttttt cactaaaaga
     2101 gtatttgaga gagccagaga aaaaaattct attgaaaaca gtcgaaggac ctaaattaac
     2161 caaagaactt tggtaataaa gaaaaagaga gagaaagaga tgtttgaaaa caaaaaaatc
     2221 aaaatcaacg taagtgactt ccagaaagta tcagctgtaa cggctgttat gatgcaaacg
     2281 atattgagct ttgctttggc aaacagccac agcgcaacaa atacagcttt tcttggtacc
     2341 ttttacgtca tcgttaaata cacagcacca atgtttattt ttgcaattgt ttataatatg
     2401 gtaaaaacaa gtcagcattt gtcttattgg gaatttttga aagaaaagtt ttttgaacta
     2461 gtcgttccgt atcttctatg gacgacggct tatctttggc tatttccagc agtccaacaa
     2521 caaactccgt atacggatac tgcaagcttt ttactgaaat atgtcacagg tgatggagcg
     2581 cctcatttat ggtataccgt tatgatgctg cagatccaat tgttcatgcc gttttttgtt
     2641 tggctcggtt acaaagtatt tgctaataaa aaatatgtat ggccagtatt gattggttca
     2701 acgattttat atgtcgcatg gtatctgttc tatcaaacac aagtattgac tgggccttat
     2761 catgacagct ggtacttatt agatcggttt gttgctagtt tcatgattta tggggtagcc
     2821 gcatttgttt atcacgaaaa agtctatcaa tatttggaca gagtacgtta tcttttctta
     2881 cctgttgcct tagttatcgc gttcttttct gtccgttcat tgctcgcaca tccaggagac
     2941 ttgagctttg cgaatgcacc ttacttgaat acgatccaaa gtttatacag tttagtcatc
     3001 atctttgctg tcttcatagg cgcttccaaa atgattgtaa acgattcgcc aaaattgcca
     3061 ttgttcaaat ggttgtctgt ttatgcttat cgtacgtatt tagccaatgt atttgttttc
     3121 caagtactgt tattattgtt taaagatagt tggctgcagt taccttcagg agtcatgatt
     3181 cttgtagctt atctaatgac ggcaagttgt gcatttgctt taagctggtt acttcatatc
     3241 atttgggtag ctatcaaaaa aggtttcagc aagtaatagt aggaagaagg agtgagtgtc
     3301 atgataaatg acgaacgaaa aaaagcagtg actcagcaac tcgctgcgtt ggtagaggaa
     3361 gcattgatcg cagaagtaac attaagtcca aaacctggat tagtggatgc actggatgcg
     3421 ggtgcacatt ccgatatgga ctatgcttta tttcttaagt ctgctaagac gctaactcct
     3481 ttttttgaag aaatggctca aatagcatgg catcatgcta ttgatcagga gttaagagaa
     3541 agaattgctg agattgggcg aaaagcggaa aaggcaatgc tgattgctac aaatggcatc
     3601 aatacccata aaggagcgat ttgggcaatg gggctattga tcagtgtcat tgcacaaaaa
     3661 atcagtatgg aacagccttt ttctttatcg aaaatacttg atgatgtcag caagcttgct
     3721 gcttttcctg atacaaaata tcgtcataaa aaaaccactc atggtcatag ggtgaagcaa
     3781 aaatatggcg taaatggtgc atatgaagaa gcgatattag ggtatccgca tatcaggctt
     3841 gcaattcaag cagctaagga gctagataat gattcatatg tacagaaaca aatgcatatg
     3901 ttgttagttt tgatgagttc gctagatgac acatgcgtat tgcatcgcag tgatcagaaa
     3961 acgttagtgg aaatgcaaaa acttgctcat gctgctagtc aaagtgcttt accaaatcgt
     4021 ccatttacag cactgttgca cttttgtcag gaaaaccaaa tctctcctgg aggaagcgca
     4081 gatctcttgt ctgcaagctt gttcatgctg aagatagagg aattatggac tgccgacaca
     4141 tacatttgtc cgatgttact caatgactaa ataagacaaa attgctaaaa aaacagttta
     4201 tgcatctggt tattgactct tccttttagc aaggctatca taaaaatagt tttttgtaat
     4261 aaaagataac gaggaagaca atgaaaaaaa tcaattattt agcaatcatt ttgttagtgg
     4321 gcctatgttt gtttgctctg ccgacccaag gattcgctgt cgcgggtggt catggtggag
     4381 gtacatctag cggtcgaagt tcgggcagag gttcatttgg tggtcgttca tatgggggca
     4441 gttattttgg cagtcgttct tacggcagag attcttatgg gtattatggt ccaggtacag
     4501 gtccttctag ttatgagcaa acgggatttc ttgcattagc tgttttgtca gtaggagggg
     4561 tttctgcttt ttctatcgtc aagaataaaa gacaaaagaa aaggctgaaa gcggagatat
     4621 tgtcccgcat gtcaggaacg gctaaagaaa agaaagcaca gtttctggaa atccaaaaga
     4681 tattcatgaa gatccaatcc gcttgggaag aagaaactcc tgaaaaagcc agaagctgtt
     4741 atacagacaa gctgtttgcc gaacatcaga aagttctgca aaaaaacaaa gcagacggtg
     4801 taagaaatca tactaaaaaa ataactattc aggaagtagg aaattatcga cagatccacg
     4861 aaaatagttt tgccatccga attgatttca gatgctgtga ttattcagtc gatcgaaaag
     4921 atgggcgatt gatctcagga agtaagacac aacgacagta tttttctcag atatggtatt
     4981 ttgatttttc tgagaaagaa aaaagatggc aagccgattt tattcagccg attatgctgg
     5041 attgacagga aatatttatg aacggagttg tgacaaaagc cttgtcgcag ctcctttttc
     5101 tgaataaacg gtgtgccaga gatagcctat cggcaataag gcacgatttt gtaaaatatg
     5161 ggaagcgatt tttcaaaatc gcttcttatc gctcaaggct gaacatctct gtcacaacct
     5221 ctccactaaa tggtgtttaa aagcttgcat ctgcgataat aagaacaacc aaacgaaaaa
     5281 taggaagcac tattttagtt tagttgttct tattacttga tgcaggacag ctttttcaca
     5341 acctcttttt tgtatagatg ggcaaaattt ttgtaataat aggcgtatgt ctttttcgtt
     5401 gaggagatga aaaagcatat ggcttatttt ttgtttgctt tgcttacttt ctttccactt
     5461 caagtggcag aaatatctac tttttattat gtaagcggaa ccatatactt gaaatcagaa
     5521 ggagatcatt tctatgaaaa caagaacaat tgaaatactt cacagaatca tgacagccca
     5581 atatccagta aaagcaaaac atctagcaag tgagttcaat gtcagtcagc ggacgatccg
     5641 tcaggaagtt cttgaagcga atatctggtt gagcagtaaa aagcttccgg agatccgtac
     5701 aattcgcaac aaaggttttt tgttgaagct ggaaaaggaa aagcaagctc gattagaaac
     5761 tgaaataaaa gatgtgaaag acggcttttt gaatcggcgc gaacgtattt ttgatctagt
     5821 tttagccgtt gcttacagcc gaatcccgat ccatctaaac aaaaaagaag aagaatttca
     5881 aatctctaaa agtacattag atgaagatat gagacggtta cgagcaacac tggtcagtta
     5941 tgggatagag atcgtcagtt atggaaagca aggactggtt tataaaggtt cagaacgatc
     6001 tatccgtacg atgatctatg attttatcaa tcagaatctg ggaatgaccg acttctttat
     6061 gaatcaagat gcacaattaa caccggctca aaaaatcttt ctccattatt ttcctttggt
     6121 ggaaatcaaa aaaatacagg agatctattc caataagttg ttgaaacaag aagatgatat
     6181 ttataaaaaa caaatcctgc tttttacatt gatctggatc cagcgaatca ggaaacatga
     6241 atttatctct gccattaatt ggaaaaatac agaaataaaa gacagtagct tttctgcatt
     6301 tattgaccac atgatcgagg aatttcaatt ggacaatgtc ccgcaagtag aaagaaatta
     6361 tcttcgtttc actattgaga cgttcaatgc tagagatatc tccaattcaa tcgagtgggt
     6421 acaagctcaa ttactaacga tccaattgat ccaatttgta gagaacgaga cacacgtacc
     6481 tttccatcta aaggaagaag ctttattcga aaatctctat aagcacatgg ctgcactgat
     6541 tgtaagaatc aagaatcata tccaagtcat gaatccattg aaagaaaaca tccgaacaaa
     6601 ttatttccca atctaccgcg cagtcactca ttttgttcct acgatcgaag aagtaattgg
     6661 tggcaagata ctggaggacg agatagcttt tcttgtcatc cacttttcga cgatcgccag
     6721 tgtaatcaag cgagacttga attatgttta taaatcagtc gttgtctgta accatgggat
     6781 ggctactgga aatctgttag cagagaatct gaaagaaaag tttcctcaga tcgagattac
     6841 tgcagtttta agttcaaaag aaatcgaatt agtagataaa ttggatgtca atctggtttt
     6901 cagcaccttt caattgaatt atcgaaataa gccgctttta gttgtcgatc cgattttaac
     6961 tgagacgaat atcttggtta tagaagactt tttgaaaaga aatgaagaac tacagcggtt
     7021 agagcctaac agtcatgaat cgacggagtt attcactcaa ataatagatg tcatgaaaga
     7081 aagtggtggc cgagtagatc gaatcatcta tggcaagtta gaaaaattat tcgaaacaaa
     7141 caatttagaa atcaacaaaa gggagatcca gcctatgtta aaagatattt tgacggacag
     7201 caatatcatg atcaaagaaa aagccactac ttgggaggaa tcgattgaac tagctgctag
     7261 accattggtt aaagaaaaca ttgtcgaaga gcgctatgtc gaagcgatga ttcgtgcagt
     7321 gaatgattac ggaccgtata tcgtgattgg caaacacatg gcactagccc atgcccgacc
     7381 agaagatggg gtcaataaat taggagtcag cgtagctacg attgagcaac caatcgattt
     7441 cggtaatcct gaaatggatc cagtaaagat tattttttgt ttggcagcag tagactccta
     7501 ctcgcatctg aacatcatga aagaactaat tgaattgatc aacaacgaag agaagttgga
     7561 tcgattgatt gaatgcacaa atatcgaaga tttcaaacaa ctgctttttt cagtagttga
     7621 gaaataataa atgatacggg ggagaacaaa gatgaacaaa ttgaacattt tattcgtatg
     7681 cggtgcaggt ttaggaagta gttttgcagc acagatgtct gctgaggatg ttttgaacaa
     7741 attaggagta gaagccaaac tggaccattg tgatatttct tccgcagttt ccatgaaacc
     7801 agatgtgatc atcacagcag aaaattttcg ttcgcagttc gaaaagttcg cagttgatcc
     7861 taaaacggcg atgatctatt tgaaaaatat tgtttcaaaa aatgaaattg aagaaaaaat
     7921 caccccagtt ctgcaagata agggagttat ctaaatacaa aaagaaaaga gggtaaaaac
     7981 atgggcgtta tcaattttat catcgaaaat attttgactc aggcatcgat caccattgca
     8041 ttgattgcta tgttgggtct actactgcag aagaaatcag caggacaagt catctctggc
     8101 acgttaaaga cactcctagg atttcaagtg ttgagtgccg gttcgagcat catcgttgga
     8161 agtttaactt atttcgggaa aatctttact gaaggatttc atatgcaagg gatcatccct
     8221 tctattgaat ctatcaatgg tcaagcaatg aatgatttgg gtttaggtag agatattgca
     8281 ctgacgttct tagccatttt tgtattcaat attattttgg ctcgtttcac aaaatggaag
     8341 tacatttttt taactggtca agcaatttta tggatggcaa cgatgacgac agtctttggc
     8401 tattttgccg gtctgcgagg gatcgtattg atcttggtcg gtggttttat cggagcttgt
     8461 ttcgccattg ctatgccggc agtagcgcaa ccgatcattc gtaaaatcac tggatcaaat
     8521 gatattgctt taggtcattt ttgtacgatc gggtacttat ttgaagccgg tgttgctaag
     8581 ctttttggtg agaaaggcga aaataaaaag tcgatcgaag atatcaagtt gccgacacat
     8641 ttcgaatttc tgcaagatac ttatttgtct gtcatggtcg tgatggtccc gctatatatc
     8701 atcactgttt tatttgcagg cgaaccattc gcttctgaat tatcaggaga tcaaaactat
     8761 atcatgtttg cctttttaca agccatccaa ttcgtagtgg gtgtgtatgt attgctagct
     8821 ggtgtacgtc tactgttagg tgaaatcgtg ccggcatttc gagggatagc tatgaaatta
     8881 gttcctgatg cgatccctgc cttagattgt ccagttttct ttccttatag cccaaatgct
     8941 gtcattttag gtttcattac gacaacgatt ggtacgatta ttgccatgtt tactttgccc
     9001 atgttcggct tagcgatgat tttacctggt atgctaacca atttctttgc cggagggaca
     9061 gcagggatat ttggcaacgc agttggcgga cgccgaggag cgatcattgg cggtattgct
     9121 catggtttct ttattacatt attgcccgct ttgctagtaa cgatctttaa ttcgatggga
     9181 ttcaacaatg ctacagcaac cgatgtagat acagtagccg cagcactctt atatgcctgg
     9241 atcttaagcc cagttttgaa agcattttaa ttgaaagtag ggaaaatgat gtttggattt
     9301 tttaagaaaa aagaaaaaga accgaaaaaa gttctggcaa aagaagaaaa agccctatcg
     9361 ccagaagcga ccgaaaagat tcaatcagag attacacaat tgaaacaaga gatttccact
     9421 acccaagata agcataaatt agcgaaactt tatgaacaag ttggacttaa attttctgaa
     9481 ttgtatgtga acgatcaagc catccaatat ttagaaaaga gtttggaaaa taaacagacg
     9541 atcggagatg ggtataaaaa actgatgagc ctctataacc agaagcgtgc agatgctgct
     9601 cgtgctggtg atgatcaagg gatcgattac tacatgggca aaatggatga gatgcgacaa
     9661 attgcaaaac aagtaacgat caaaggaaac aaataaaaag ataaggtggc aataaaagat
     9721 gtatacaaca ttgaaagaag taacaaaaac agcagaagag ttgaacttta cagttggagc
     9781 ttttaatacg cataacctag aaatgcttcc tgctatgcta ggcgcagcaa aagaaatggg
     9841 ttcgccgatc attattcaaa caagtgtcga taccgctaaa tatatcggca tgcatgtgtt
     9901 agtcaatgta atgacagcta ttgcagaaga agaaatggtt gatgcagtct tgcacttaga
     9961 ccacgcccga gactttgatg atatcaaaga agcaatcgac agcggatata cttctgtcat
    10021 gtatgatggg tcccatctgc ctttcaaaga aaatatcctg aagacaaaag cagtagtaga
    10081 atatgcccat gcgcacggtg tttccgtaga aggagaactt ggaacaatcg gcggtacaga
    10141 agaaggaatc catgtcgctg aaaatgacaa ggtatataca agaccggaag acgcagtgaa
    10201 atttgtagaa gctacaggcg tggatgcatt agctattgct atcggtacga atcatggaca
    10261 attcaaatca aaaacagaag taaacattcc cttgctgaaa gaaatcgatg cagtagttga
    10321 tgtcccccta gttatccatg gaggaacagg tgtgaaagaa gaagattatc ctgaattgat
    10381 caataacggg atccgcaaat tcaatgttgg aacggagctt ttagtcaact ggacgaaagt
    10441 agctaaagaa acctttagtg aaacagaaat caacaagtct cttcgccata atgtgattcc
    10501 agcaaatgaa gcagtaaaag aaattgtcaa gcataagatt ggactgttta tgaacttgga
    10561 aagtccaatt aacttaggaa ataaatgaga tgaaaaagta tttcattctt ctggctggta
    10621 gtcctggtac aggaaaaaca tatcttatga ataaactacg ggaacgattt ccagatatgt
    10681 atgccatcac gctcgatgaa atcaaggaat actatgcaga atccatcggg tttaacaatt
    10741 tggaagaacg ggcggaacaa gaaaggacga aagtttatcc tttctattac aaagcacttg
    10801 atttgtatat ggaagcagga aagaaagtga ttgtgtccga gtatcctttt agtgataagc
    10861 aaaaagcacg actccaagaa ttagcagata gatacggata tcaggtgatc acgattcgcc
    10921 taacagcaga ctttgaagtg ttatggcaac gtcggtacaa acgtgatcgt gaatcagatc
    10981 gtcatttgag ctatatcatg gatcattatc actatggtga cgtattggaa gatcgaacga
    11041 aagggaccaa tcatattacg aaagaagcat tcaaaaagat catcgccgac cgaaaatatg
    11101 acaaattcca gttaggaaca ctatatgaat ttgatgtaac agactatact caagtggatt
    11161 acactccact attggataaa ttagaaaaag aaatcaatga caataagtaa caaagaaatg
    11221 gcttaatatc gaaaaattgg cttttgaacg ctgtttaccg agaggtcgtg agcctgtcgt
    11281 tcagctccga gtcacaagga gcaatttcaa aaaaacagct t
//
LOCUS       DBNICI010000095        11255 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.095, whole genome shotgun sequence.
ACCESSION   DBNICI010000095 DBNICI010000000
VERSION     DBNICI010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.095"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(48..2057)
                     /gene="metG"
                     /locus_tag="AC1ZCC_001942"
     CDS             complement(48..2057)
                     /gene="metG"
                     /locus_tag="AC1ZCC_001942"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIQ1630187.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            2325..2603
                     /locus_tag="AC1ZCC_001943"
     CDS             2325..2603
                     /locus_tag="AC1ZCC_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630188.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            2700..3521
                     /locus_tag="AC1ZCC_001944"
     CDS             2700..3521
                     /locus_tag="AC1ZCC_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIQ1630189.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(3806..4816)
                     /locus_tag="AC1ZCC_001945"
     CDS             complement(3806..4816)
                     /locus_tag="AC1ZCC_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HIQ1630190.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(4828..5781)
                     /locus_tag="AC1ZCC_001946"
     CDS             complement(4828..5781)
                     /locus_tag="AC1ZCC_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1630191.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(5771..6841)
                     /locus_tag="AC1ZCC_001947"
     CDS             complement(5771..6841)
                     /locus_tag="AC1ZCC_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1630192.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(6847..7890)
                     /locus_tag="AC1ZCC_001948"
     CDS             complement(6847..7890)
                     /locus_tag="AC1ZCC_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1630193.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(7891..8832)
                     /gene="opp3b"
                     /locus_tag="AC1ZCC_001949"
     CDS             complement(7891..8832)
                     /gene="opp3b"
                     /locus_tag="AC1ZCC_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HIQ1630194.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            8916..9263
                     /locus_tag="AC1ZCC_001950"
     CDS             8916..9263
                     /locus_tag="AC1ZCC_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HIQ1630195.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(9333..10994)
                     /locus_tag="AC1ZCC_001951"
     CDS             complement(9333..10994)
                     /locus_tag="AC1ZCC_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HIQ1630196.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            11173..>11255
                     /locus_tag="AC1ZCC_001952"
     CDS             11173..>11255
                     /locus_tag="AC1ZCC_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HIQ1630197.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQK"
BASE COUNT         3178 a         2287 c         2051 g         3739 t
ORIGIN      
        1 ggaaaaaggg ggtatgacac aactagtgtc ataccccctt tgaatttcta tgcgatttct
       61 gagccatttg gcatggattt tggtgcttcg attacttgta aagagccatc tggtgcttct
      121 gcagaaagga tcattccttg actgatttgt ccacgcatct ttcgtggttt cagatttgct
      181 acaatgacga cttttttccc gatcaattcg cttggatcag gataaaattc agcgattcct
      241 gacaagattt gacggtcttg gctgtctcca gcatctagtc ggaattgcag caacttatct
      301 gccccttcta ctttttgaca gttgatgact tctgctactt ttagttctac tttttcaaat
      361 acatcaaatt tgatttgttt ttctttcgtt gaaacaagtt ctgtttcttc tggatcccat
      421 ttgactgtat cttcgtttgt ttgagtacct tcagacattt tttcttgaat aaaagcaact
      481 tcttcttcca tatctaaacg tgggaagatt ggtgtacctt ttgcaactac ttttgttcct
      541 gaaggaaatt cgccaaagtg taatccttca agattcattg tttcagagtc caatcctagt
      601 tgattgaaga tcttctctgg cgtttctgtc atgattggct gaagtaatat cgctgcgata
      661 cgcaaagatt cagctaaatg gatcatgaca ctattcaatt cagctttctt ttcttcgtct
      721 tttgctaata cccatggttc tgtttcatca atgtatttgt ttgcacgaga aatcagcacc
      781 cagatttcag ccaatgctgt attgaattcc attttttcca ttgcatcatg gtaacgtccg
      841 acaacgttag cagccgtcgt agacagttca ctatcaaatg gagtgacaag tgaggcatag
      901 tcaggaacga gtccatcaca atatttgttg atcatagcaa tcgttcgatt caataagttc
      961 cctagatcat ttgccagatc ataattgaca cgggaaacaa aatcttctgg cgtaaagaca
     1021 ccatctgacc caaacggaac agcacgaagc aaataataac ggagtgcatc taaaccataa
     1081 cgttccacaa gcatttcagg ataaacgaca tttccttttg atttagacat ttttccgtct
     1141 ttcatcaata gccagccatg cccaaatact tttttaggta gaggcaaatc aagggccatc
     1201 agcatgattg gccaatagat cgtatgaaag cgaacgattt ctttaccgac catctgaaca
     1261 tctgcaggcc agtatttttc gaatagactt tcatcttcgg agccatatcc tagtgcagta
     1321 atataattgg ataatgcatc gatccataca tacaccacat gtttcggatc actcttgact
     1381 ggtactcccc aagtaaaagt tgttcgtgaa actgcaagat cctctaatcc tggtttgata
     1441 aagttgttga tcatttcgtt tttacgagat tctggctgga tgaattcggg atgttcttca
     1501 tagtattcca atagacggtc tgcgtatttg ctcatgcgga agaagtatga ttcttctttt
     1561 accaattcta cttcgtgtcc acttggcgct ttaccaccga tcacgttacc ttcgtcatct
     1621 ttgtacactt ctgcaagttg tgtttctgta aagaattctt cgtcagaaac agagtaccag
     1681 ccttcatatt cacctaagta aatatctccc tgttctaata aacgatcaaa tattttttga
     1741 acagctgctt tatggtaatc atctgtcgtg cgaatgaatt tatcataact gatatcaagc
     1801 gttttccata atttcttgac atcagccgcc attttatcta catattcttg cggttttaca
     1861 cctagttctt ctgcttttgt ttcgattttt tgaccatgtt catccactcc agtgagataa
     1921 aacacatcaa agcccatcaa ccgtttataa cgagcaattg cgtcacatgc aatcgtagta
     1981 tacgagttgc cgatatgaag tttgccgctt ggataataga ttggcgtagt gatataaaaa
     2041 gtttcctttt ctgccacggg gctcttcctc ctttatcgca tgatgcgaat tcgtttcctt
     2101 gctagtatac cataaaaaaa gcatgctgtt agcttttttt caaaatgaaa aagactggat
     2161 aaacgacttc tatctacgtt caatctatcc tttaaaagga tgaataggtt gaataaatgc
     2221 gggtttttgt tttgtttcat atctactaaa aaacggcttt ttatttctcc cctcacacgt
     2281 ggtataataa agactatcta tcttagtaag ggactggaac aatcatgcag aagaaacatc
     2341 ttttttttac acttagtatt gcttttcttt cattagcaca tttaatattc agctattttt
     2401 atattcgtat gtatggttat tttaacctcc atgggcattt gaacagtttt atgacagcag
     2461 cttggatttt gcgttttatc attgacgtat atatcgttat ttgtggcttc tttgccattc
     2521 gtgaagagcg gtataaagta ttgccgtttt atctgctttt ctttttattc aatctcatac
     2581 ttccatttat ttttcatatt taaatgataa cctagatttt tcaagtaaat ccttggtttt
     2641 ttttcagagt ctgctacaat aaggtaaaaa gtacttttac tgtcaatgga ggtggtcaga
     2701 tgaaccgttt tgcttctaaa gaagaagaaa aattatatta tgaacataaa gcaagtgaag
     2761 aagaatttct taactggtat caaaaacagg aacgtccaga atatgacaag ccctctttga
     2821 ctgtcgatat cgtactgatg tgctataaca aagaagaaga tcagctgaaa gttttgttga
     2881 tccaaagaaa agggcatcct tatcgaaatt catgggcatt gcctggtggc tttgtccaaa
     2941 aggatgagtc aactggagaa agcgtcttaa gagaaacaaa ggaagaaact ggtgtggtta
     3001 tttcaaaaga aaatattgag caactgcata cattcagtac gcctaatcga gatccgcgag
     3061 gctgggtcgt cacagtcagc tacttagcct ttattggaga agaaccgcta atcgccggag
     3121 atgatgccaa ggaagtacgc tggttcacac tagaacgtca tagtaacaag atccatttat
     3181 ctagcggaga agtggctatc tcgcttgatt tagtcactgg agagtcttct ggcaaagata
     3241 ctttggcttt tgaccatagt caaatcatct tgaaagcatt caaccgtgta gtcaataaaa
     3301 tggaacacga gccgcaagtt ctacaagttt taggtaaaga tttcacgatt acggaagctc
     3361 gaaaagtatt tgctaaattc ttagggatcg attataaaac gatcgatcat tccaatttta
     3421 agaaagctat gctccaatat ttcgatgaga tcggtgaacg tcctgtagga atcggccgtc
     3481 cttcaaaaat ctatcagctc aagccggggc accatgaata aggttgtgag caaaagattc
     3541 agacctaatc attcaagaaa aaacgcaaaa acaactcttt gtgagttacg ttttttgttt
     3601 cagaattttg atgtattcgt aaactttctg aataaaaaat taacaatagc agaaaaagct
     3661 cgtacgaact ttttctgcta ttgttttatt ggtttgtcat aattagtgag cttttcttca
     3721 aaagaaaaat tcctagaaac aaaagtcatt aagacttttt attaacaagg ctaaccctaa
     3781 cgctcctatc agtttcattc attttttatc ccttgataac cgaatgtctg cccatttttt
     3841 ctactacttg gctctttctt acggaattgc tttcttctgc tccgtcggta ttgatcaaaa
     3901 gaaatttttt attataccat tgggacttat aacgcaattt tgttattaat ttatgccagt
     3961 cgtgaataga aaaatcagca tgtgcctctt cttttggaaa agattctaca aataagtagt
     4021 catattgttt gaccaatttc ccagataatt gttcaagata atcttctcgc tggttctttt
     4081 gtttcagata aagatccatt acttttctct tttgtttctg ataattgctt gcctgatcta
     4141 acctgatctt tctatggtga gcggagcgga ctttcacatt caatttccgc tgtgcatgtt
     4201 gcagcttttc ttctgtctgg tgaaattttg gtaaagttac ttctagaggt cgagatgttt
     4261 ggatcaatgt cttcggatga taggcgatcc cgatccattt agctaaagat tcttttttta
     4321 ctggcgaaac atcacaaagt agtgcgacat aaaattcttc attatatcga gcagaaatcg
     4381 tagcggagcg aatcgatcct tcaactggcc gatgctgatt tatcttgatt agttcttttt
     4441 gttttggtaa ctttaagtag ccattttcca gatagatcgt acgggtttgg ttgttggtcg
     4501 tataagattg ccacatactt tttttcgttt taagctttgg gaagctggca cgtcccttga
     4561 aataattgcg aaaagcccga tccaaattaa gctgtgcatt tgctaaagca agactgtcta
     4621 cttcttttaa gaatgggtat tcttttttta atgtagcggg tgtgagcgaa aaattggcta
     4681 ctcctgtttg ttgccaactt ttctgccgct ttttcagcaa atggttatag gtaaatcgag
     4741 cacatccaaa agtacgaatc aattgctgtt tttgttcttc tgtcggatac atacggaatt
     4801 taaaagcttt tagcatctat gtccctccta gttagcaacc agaagagatt ccttttgtcg
     4861 ttttagtttt tttgcgtaat atgggacttc ctgctcagtt gcatagacaa aatgttctgg
     4921 tgcgatttca cgaagttgac gtacttgtcc gtcatctggt tcaggttgat atttgattct
     4981 tctgcgtttt ctctcatggt ctggatctgg tagaggaata gcagataaca aactttctgt
     5041 ataaggatga acaccgtgat tatagatttc atcacttgtt cccatctcta ataacaagcc
     5101 gttatgcatt acaccgattc ggtcactaat atgtttaacc atcgacaaat catgtgcaat
     5161 aaatagatag gtcaattgat gttctttttg gagatcttgc aacaaattga ccacttgggc
     5221 ttggatcgac acatctagtg cagaaattgg ttcatcacaa atgataaaac gcggttttac
     5281 tgccaatgct cgtgcaatac caatacgctg gcgttgccca cctgaaaatt catgaggata
     5341 acgagtaccg tggcttggat tcaatccaac agttctcaat agatcatcga ctttttttgc
     5401 acgctcttca gatgatttag ctagtccatt aacatcaatt ccttcggcaa tgatatctct
     5461 gactttcata cgaggattca acgaagcgta tggatcttga aagatcatct gaacatcatg
     5521 ccggaatttc gtcaaacctt ttttatcttt gatcttcgtt atatcttgac cgtcgaataa
     5581 tatctcacca tcagtaggtt cattcaagcg aatgatcgta cggccagtag tggattttcc
     5641 gcttccagat tctccaacta aaccgaatgt ttctccttca taaatgtgaa agctgatgtc
     5701 atttacagca tgtacttcat tcttttttcc tacattgaaa tactgtttca atcctttaac
     5761 ttcaagtagt ttatttggca tcagcaattc ctccttgacc gtaagtatgt ttttgtttct
     5821 ctttaaagat agcccagcga cgttggattt cttctggcgg tgtcactttg ggtgcctgcg
     5881 gagcaagtag ccaagttgct gccttgtgtg tttttgatag ttcaaaaaat ggtggttctt
     5941 gttctgcatc gattttcatc gcgaattcat ttcttggata gaatgcgtct ccttttggcg
     6001 ggttcaataa atccggtgga gaaccaggaa tagcatacaa tcgatcgttc gcactatcta
     6061 atgtcggcat tgaaccaagc agtccccaag tatagggatg ttgcgggttg tagaaaattt
     6121 cttccgatgt tccaacttca acgattcttc ctccatacat gacagcgaca cgatcagcga
     6181 cattcgctac tacaccaagg tcatgagtga taaagataat tgatgtatcg atttttgtct
     6241 gaatttcttt cagcagctcc aaaatctgtg cttgaatagt cacatcaagc gctgttgtag
     6301 gttcatcagc aatcaggatt tccggataac ataccagtgc aatcgcgatc acgatccgtt
     6361 gacgctgacc tcctgagaac tgatgcggat aactgttgat acgtttctca gcttcgggta
     6421 ttccaactaa tttaagcaat tctaatgcag ctttttgtcc ctcttttttt gaaactttat
     6481 tatgctttct aagggactca gcgacttgct tcccaatggg catcgtcgga tctaaagagg
     6541 tcattggatc ttggaaaatc atcgctattt ctttaccacg gattttttgc atctctcttt
     6601 ctgattttcc aacgatatcg ttacctttaa agaggatttg accagcatct atattggcgt
     6661 tgcttgacag caagcgcatg atgcttctgg tcgtcactga ttttccactt ccagattccc
     6721 caacgatcgc caatgtttct cctttattca aatgaaaatt gacaccgcga atcgctcgta
     6781 cctttccagc atacgtatca aaagagattt ctaagtcttt cacttctaaa attttcgtca
     6841 tcgtcttcac tctttcattt taggatcaaa ggcatcacgc aatccatcag caagtaagtt
     6901 aaagccaatc atgataaccg ataaagttac tgccggaatc cataatagat aaggcagata
     6961 caggaatgtc ttgtaccctt cgctcaacat cgttcctaat gaagctgacg gaggcgtaag
     7021 acctaaaccg ataaagctta aaaaggcttc gaagaaaata gcagatggaa tactaaacat
     7081 catttgaata ataatgacac tagagatatt cggcaaaata tgtttgaatg caattttcat
     7141 tcttgattca cctaatgtca atccagctaa aacatattct tgatctttta atttcattgt
     7201 ttgtgcacga acgatacgtg ccattggtat ccagtttgtg atggccatag cagccacgat
     7261 tgaaaaaatt cctggttcaa aaacgaccaa catcaaaatc atgacaacga ggtttggaat
     7321 tcctgataag atttccaaaa aacgttgcat cacgttatcg acacgtccgc ctaacaaacc
     7381 agaaaccaac ccgtaagcta caccaatcgt tacatctagt aatgcagcaa taaatgcgat
     7441 cagcaacgag attctcattc ccataaataa gcggcttaat acatcacgtc ccaaaccgtc
     7501 tgttcctaaa aagaaattta cgtttgaagg gacgtttgct tgagcatatt tatcaacaag
     7561 ttcaccacca accattgctt ttccattcaa tccatcgata ttgattcccg gaatacgagg
     7621 aggcaaattg atataagcca cgttttgggc agttggatca tgaggagaaa caaagattgt
     7681 gatcacagag atcaaaatga tgaccaatag taaaaccatt gaaataacgg cagctttgtt
     7741 tttctttaaa cggcgccatg aatcttggat aaaattcaaa gaaggtgcag taatctgttc
     7801 acgttcttct tcggtatttt tttgtaatgg aagaaattca tccgcaggga tatctgcaac
     7861 tgtttcataa cgctttggta tcatttccat ttatcctcgg ctcccttctg aaacacgaat
     7921 tcttggatca actagaccgt acaatagatc cacgatcaaa ataacaaaaa tcagcataaa
     7981 tgagtaaagg atcgttacag ccatgatcgt aggataatca tttgttgtga ttgacttaac
     8041 gaattgttcc ccgataccag gaatagcaaa gatattttct accaccagtg atccggtcat
     8101 caatgctaca gctaacggtc ctaataaagt catcaatgga atcaaactat ttcgaagacc
     8161 atgtttaaag gcgatttccc aacggctcaa tccttttgct tttgctaatt ccacataatc
     8221 actatgcagt acttccacca tttccgtacg gataaaccga gcagagtcag caagcggcga
     8281 catggccaac gcaattgttg gtaagattgt gtacacaaag ccatcccatt ttgcgattgg
     8341 caaaacttgt aatttgattg caaagatata ttgtaataaa acagcaaata caaagtttgg
     8401 aatggaacga cctaatatcg ctacgagtgt agaagatgta tctgcccatg tattttgctt
     8461 catggcagag atcgttccta aaatcgtccc taggactgtt ccaaagataa ttgcctgtaa
     8521 ccctaactgc aaggatggtc cgattcttcc agctaacaaa tgtgcgactg gctggttttt
     8581 aaattggaag gaaattccaa aatctccttg aagcaaatta gaaagataaa tcccatattg
     8641 tacgataact ggtttatcca aaccgacttg tttatttagc atctcgattg tttcaggact
     8701 caaacgttcc tgattcgtat atggcgtgcc cggcagcaat tgcatcaaga aaaaggtgat
     8761 tgttgcaatt aaccaaagcg tgatgatcat gaaaaagaca cgttttaata catatttgat
     8821 ataactattc actaatgttt ccccctagct tttgacttct gacagatgtt tcgctacatt
     8881 attagataat gaaccaaaaa taaaggagca atcaaatgaa aaaaaaatct atcccctacg
     8941 cagtagcttt tctactgatt ctggttattt taataaaaaa tctcatcaac cattccttta
     9001 cactcattca attatctaat gatctattct tatggtcatt gccgtttctg attatcggcg
     9061 gttttttatg ggtcttttct tcgggtttct tcgatcattt tcagcgatcg gtccatcttg
     9121 cacgaacaag gaaccgaaag aaaaaaccag aattttccac gctctcgtct gccagctacg
     9181 gtatgtacag cttttggctg atcattgccg gaatactaat tgctctttct gctattttca
     9241 tgcttttttc tttgttagga taagtgaata tgagaacgag gttgcgacat ttgccgcagc
     9301 ctcgtttttt ctcatgctca cggtattaac gattattctt caacgtatgt ccatttgtaa
     9361 tcccatgaag cgcctgccgc atggtgaacg atacctttga ttttttcatt cactaggtga
     9421 ccttctgctt tttggtacac cggaacgaca cccatatcat cagagatgat cttgattgct
     9481 ttttggaaat catcccaacg ggcttgttca tcgccagcgt ctgttgatga agctgcttta
     9541 actgcttcgt catattctgc attgctccag cgaccacggt tgtatgaatt acctgtgaca
     9601 aacaaatcag taaagctgct tgggtctgca tagtcagcag cccatccgcc tagtactgcg
     9661 tcaaaatcac ccgaagttga gcggtcaaga cgtacagaga atggaacagg tgttggtttg
     9721 actgttacgc catccaagtt ttcttggatt gagttttgaa catattcgat tacttttttc
     9781 gatgaatcat catcagaagc catcaaatca aattctaaag aatcaacgcc caattctttt
     9841 ttcgcttttt cccaatattc ttttgcttta tctttgtcat aagaaactaa ttttccagct
     9901 tcttttgcaa agtcttcgtt cgttgttgga ctttttacca tatctgatgg gatcaatcct
     9961 gtagagacaa ctgatccgtc acctagtact tgttttacta aagcttcacg atcgatagaa
    10021 taagaaatag cttttcttag gttcacattg ttgaatggtg aattcttttc acgttgattg
    10081 aattcgatat agcttgtccg cgcttcttta acagaagtat aagctggatc gtttgcgttt
    10141 tgctgtgcta actcaccaga caagatcacg tcatctgctt ggccatcttt gaataagttc
    10201 aatgcagtag aagattcttt tactacactg actttgattt cagaaagttt tactgtatct
    10261 ttgtcccagt aatgatcatt tttcttataa gtccattctg tatctgttcc tggtccatca
    10321 aaatcttcta atgtgaatgg tccattataa acagctttat cacttgcaga tgcataggca
    10381 tccccatttt cttccactac ggattgtttt tgtgggaaaa atgatgggaa agcaagcaga
    10441 tattggaaat atggtgtttg tttcgtcaat ttgatttcta attcatagtc accaactgct
    10501 ttgatcccta gttctgattt atctttcttt ccagctgtaa tgtcttctgc attttcaact
    10561 ggcgcataca ggtaagcata ttcagaagct gtagctgggt ctactgttcg ttgccagcca
    10621 taaacgtaat ccgctgctgt aacaggatca ccatttgacc attttgcgtc ttctcgtaat
    10681 ttgattttgt aagttaatcc gtcttcactg acttctgcca actcgctagc tcctgccggc
    10741 tgtggtttgc tgtcttcatc aagacggtaa attccttcat aaacgttatt caatgctgta
    10801 aaactgattg tatctgttgc aactgataag tcagctgtag gcatttcttg aggcacgact
    10861 aagttaaata cgcctcctcc atctgctgaa ccgctttttg ctgaactact gcttgtggct
    10921 gaactgccgc cataacatcc agcaagtaca aggccgcata ccgcaaccaa accaaatgta
    10981 aatttctttt tcattttacg taccccttta ttggaatttt cagaataatt aatattttct
    11041 gaagttgttt aagagtttaa tgaataatta ttaaaaaatc aacacttttt tttgtctttt
    11101 tcataaaata atgatttttt cgtgaggttt caaggaattt ttcagaaaaa atagctttgt
    11161 acaaggtttt ccatggtctt ccaaaagcca ttaaataatt ttcgttatat tgtctattat
    11221 tgccatacaa agataaaaaa gaataaacaa aaaga
//
LOCUS       DBNICI010000096        11223 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.096, whole genome shotgun sequence.
ACCESSION   DBNICI010000096 DBNICI010000000
VERSION     DBNICI010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.096"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            201..680
                     /locus_tag="AC1ZCC_001953"
     CDS             201..680
                     /locus_tag="AC1ZCC_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HIQ1630198.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            713..2941
                     /locus_tag="AC1ZCC_001954"
     CDS             713..2941
                     /locus_tag="AC1ZCC_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIQ1630199.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3070..4713
                     /locus_tag="AC1ZCC_001955"
     CDS             3070..4713
                     /locus_tag="AC1ZCC_001955"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_function: GO:0004574 - oligo-1,6-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009311 - oligosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HIQ1630200.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4786..5331)
                     /locus_tag="AC1ZCC_001956"
     CDS             complement(4786..5331)
                     /locus_tag="AC1ZCC_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HIQ1630201.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5354..5839)
                     /locus_tag="AC1ZCC_001957"
     CDS             complement(5354..5839)
                     /locus_tag="AC1ZCC_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIQ1630202.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5829..6641)
                     /locus_tag="AC1ZCC_001958"
     CDS             complement(5829..6641)
                     /locus_tag="AC1ZCC_001958"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009229 - thiamine diphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HIQ1630203.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6780..8312)
                     /locus_tag="AC1ZCC_001959"
     CDS             complement(6780..8312)
                     /locus_tag="AC1ZCC_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIQ1630204.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8421..9452)
                     /locus_tag="AC1ZCC_001960"
     CDS             complement(8421..9452)
                     /locus_tag="AC1ZCC_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIQ1630205.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            10176..11162
                     /locus_tag="AC1ZCC_001961"
     CDS             10176..11162
                     /locus_tag="AC1ZCC_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HIQ1630206.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         3805 a         2035 c         2179 g         3204 t
ORIGIN      
        1 attccaacat catcttggac tttctacatg ttactaatga gcgtgtaaaa gaacgagatg
       61 gtgtttttat ttctatcaat gaagctcttt ttctttgcaa tcctcacttc aatcctctat
      121 aatgtcgcat atagtttagt aaatgagtaa actactaatt tacttattta ctaaaaataa
      181 aaggatgagg agttaaaata atggaaatac caacaaattt aaaacataaa ccagtaatcg
      241 ttgtagaaga ctatgataaa gttgatggaa gaaatgcatt aaatatggat gcaaaagggc
      301 tttcactggg ccttgctcag tggaatgatc gtggaaaagt cgatatctca ggcaaggtat
      361 ggcgacatac tggagaaaaa tggtcacgac agtcggaaga attaccaatc acacgtattt
      421 tagatttagc gatcttagct gcgcaaggaa gtttatactt ccaagatgct tatcgttatg
      481 aaaaatttta tgatccagaa aatccagtcg tagacatcat tggattacaa gggaaccgaa
      541 tgacggtaga agtagatacc aaaaatccaa agatcgatga agacatcatt ttgtattacg
      601 atacactaca aaaagacgga gaactgattg gggaacgatt ccgtatttta aaacgtcttt
      661 tagatgagct aggatattaa tagacagaga tgatcaaaag gggaagagaa gcatggagaa
      721 tttctcacta acagaaaacc gaaccttttc acggctagaa aagcaaatga tctggaagaa
      781 accgaaaagc catcggacaa gtgcagaaga atggcggatc gcctccgaaa tcaaagggaa
      841 ttggcaagat cctgaaatga aaatcagtaa cgtacttttg gaaggagatg ccgggtcggg
      901 gaagacacaa ttagccaaag cactatctgc tgatctgcaa cttccctata caaaagtgac
      961 ctgttttgca gatatggaca aatcagatgt tttcggagcc ttattacctg tagtagattc
     1021 taataaggaa caagaccaag aattactaga agctatttac caaacagata cacttgaagc
     1081 tgtactgaca ctgatccaga accattacgg cattgatcct ttttctgcta aagaaaaatt
     1141 agcagagcta gttcaaagga tcgaagctga ttatccagat gcaatccatt acagctatta
     1201 cccttctgaa atcgttcgag cgattgaaaa aggctattta ttagagattc aagagccgac
     1261 tgtcatccga gatgcctctg ttcttgtggc acttaactca gcgctcgaac caaatgggat
     1321 gttgaattta ccgacaggaa tcattcgtcg ccatccagat tgcgtcattg tgatcactac
     1381 aaatcgaaat taccaaggaa atcgcccatt aaatgaatct ttaagagatc ggatgcaaca
     1441 tgcggaaaaa atggatcttc ctgccttaga agtcatggca gaacgtgcga tggcgaaaac
     1501 tatgatccaa aaacaagaac ttctcttaaa gatggcagag attatccgtt tgttggatga
     1561 aactgcaaaa gctaacgcca tcaaaggtgt agcaggaatg cgttcttatt tttattgggc
     1621 aaatacctat aagcaaggac aagatttact ccagtcgatt tatccaaaag ttttgtataa
     1681 attatccact gattcagatg aattggcaat tcttgaacaa gctttgtctg acagtggatt
     1741 gctactggaa cttcaggaca tattgagaaa agaaaaatgg ggtcatcctc aatctgagca
     1801 tacaaaagaa agaaccatta gcgccgaaga agcaaatgaa cgaaacatca ctgaaaatgc
     1861 tgaagaaatt ctgcgcttag atgaaaagaa atcgttgtct gctgaagaac caaatgaggg
     1921 agaagagaaa caagaagaaa caatagaaaa aaacgagatt cctcctcaaa tgcaaaaaga
     1981 acgcagtagc gatatcgaag gaaaaaatca aaaagaacaa caaacaggag atgattctca
     2041 agaaatgtct gcttctgaca tggaagcaca tgataaggaa atcaagaagc aactgaataa
     2101 agaagcgcga caaatcatga aggaaacgat tcatgaaaaa gaaggtctca tcgttcaccg
     2161 accagctttc caaggaaaaa cgccggaagc acgtgagcta cacatgcaag taatgccaat
     2221 agtagaatca cttagccggc aaattttaga gttactggat aatgaacaaa cagagacgta
     2281 ccaaaaagga aaatacgaag gtcagcggtt caatgcaagc aaagtagcat atggtgattt
     2341 gagaaatttt gacaaaaaga acccgccaca tgagcaacct tctttagcgg tagctgtacg
     2401 gatcgatgaa tctgggtcaa tgatacggga tgatcggatc gaagcggcaa aaaaagcagc
     2461 gattgccatt gccgaatttt ctaagaaagt cgatatccca ttgttgattt acggagatac
     2521 agcagaccgt tcgtcaagag aaaagacttc tttgttttct tataaagaat ttgaagacgg
     2581 atttcaatgg ttaaatgaaa aattagtcac tatgaaacca aggcaaaaca atcgtgatgg
     2641 tgctgcactt cgtttagcag cagaaaaatt aaacagacaa tccgcaacaa ccaaactgtt
     2701 gcttaatatt agtgacggtc agccaaaagc gttgccggat tatacaggca aaaaagcaaa
     2761 agaagatatc cagtctgttt tgaaagaata tgaacgtcaa ggaatcctat ttgtctccgc
     2821 agctattggc caagacaaag aagaaatcaa gtcgatctac ggtgaaagtc gttttgtaga
     2881 tattactgac ttgaatgaat ttcccaagca aatgattcaa ctgattgccc gctatctatg
     2941 ataaaataaa gctcaacgaa cgcacttagc agattcgttg agcttttcgt ttctagttac
     3001 cggtaacaag gatacttcta aaatagaatc ggttataatc aagagggacc gttaaaggag
     3061 tgaaacgata tgatacaaga acaaaaatgg tggaaagaag cagtaggcta tcaaatctat
     3121 cctgctagtt tcaaagatag caatggcgat ggtcaaggag acatcaatgg gatccgtgaa
     3181 aaacttaatt atttgaaaga tcttggtatc ggctttatct ggatcaatcc gatctatcaa
     3241 tctccatttg tcgataatgg ttatgacgta agtgactatc aagatataga agagagattc
     3301 ggaacgatgg aagattttga tttattgcta aaagaagcac atgaattagg gatcaaagtg
     3361 attatggatc ttgtcatcaa ccatagttcg gatcagcatc aatggtttga agaatcgaaa
     3421 aaatctaaag aaagcccgta tcgcgattat tacatttggg tcgacggggt agatggcaaa
     3481 gaaccgaata attggacatc gattttcggc ggttctgctt gggagtattc tcatgaaact
     3541 ggacagtatt atctgcatgt ttttgcaaaa gaacaaccag acttaaattg ggaaagcgag
     3601 aaattgaaag aagaactatt caatatgatc cgatggtggt tggataaagg gatagatgga
     3661 tttcgtttag atgcgatttc tcatgtaaaa aaagatgaat attcagtaaa agccacagag
     3721 aaccctttct caccctttca aaatgtctca ggaatcgaag aacatttgac cgatttgaaa
     3781 catgtttttg aagagtacga tatcatgact gtcggagaag caagtggtgt cactgcagaa
     3841 gaaggaccac aatgggtagg gaaagacggt tatttcgata tgatattcga atttgatcat
     3901 atccatatct ggcagcaaga aaaagaaggg caattagatg tccttaaatt gaaacatgca
     3961 ttaagcgctt ggcaaacttc attggatggt atcggatgga acgctttata tatggaaaac
     4021 catgatgttc ctcgtgccgt ttcagtgttt ggagatactc gtcccgattt ttgggctatg
     4081 tctgcaaaag caatcgctat gatgtatttc tttttgcaag gtacgccatt tatttatcaa
     4141 ggacaggaaa tcggaatgac gaatatgccc tttgaatcga tcgatcaagt tgatgcagta
     4201 gattcgaaaa gactttataa aaggctgctt gcggaaggta agacaagaga agaagcattg
     4261 gatatcattc gtgaaacaac gagagacaac agcaggacac ccatgcaatg gacttcggaa
     4321 caatatgctg gtttttctac acatgaacct tggctgatca ctaatccaaa taccaaaacg
     4381 atcaatgtag aacaacaaga atatgaacca gagtcggtcc tgcaattcta taagaacatg
     4441 atccgcatta gacaaaccaa taaaggtctg atatacggtt cttataaaga atatcttcat
     4501 gaacatccac aactttatgt ctatgaaaga tatttagaag atgaacaata tcttattatg
     4561 gtaaacttaa cagaatcact tgctgactat gaacttccaa aagaagcaga tcagtcctgg
     4621 acattactct tgtcaaattc ctctagcgga gaatttgaag ctaaaggcat actagcacct
     4681 tatgaagctc ggttatataa aacaaacaag taaaacttcc tcgttaaagt tcattagttg
     4741 gactttgcga ggaagttttt cagattgctc tgtcaattac tcatttcaat aattcgctct
     4801 aggaccaccg atatatttag gacgagagcg aagggctgtc acgtgagctt gtccaagggt
     4861 tttcaaacga ggtctgacag gtacttggac atgcttgata tgcataccga ttgatgtgtc
     4921 accgatatcg ataccggctt gtccaactac gtgttccact tcgacaggat cattgaactg
     4981 gtcaaaagca gcaacagaac aagcacctcc agcatgtagt gcaggaacaa cagagacaat
     5041 ttcgaaattt tttgcttctg ccactgttcg ttctactact aatgctcggt tcaaatgttc
     5101 acaaccttgg acagctagcg caatttcttt aggatccaac aactctttta acgtacggat
     5161 gatccattga ccaacttccg cactggaatt ttttccaata tgtcctccaa cgacttcgct
     5221 tgtgctgcat cctaaaacaa aaagatcacc ttttttcaag tgtgcctcag ccaatatgtc
     5281 attaactatt tcagtcaatt ggtcttttaa ttctttttct gacaacttca ttttctaaaa
     5341 ccttcttttt caattaatat tgcttccgtg cttgaatacg ttgaagactt gcagttatag
     5401 gaaaggcaag tcccattcca atcagatttt ggaagatgtt tcctggtata gaagccactc
     5461 cagccggcca atcatataaa atccaaccgg cgataccata gccgatgatc ataactaagc
     5521 ttcctaataa aagccctaat ccccacatac gagtagaaga tttgctgaaa gaaccagcaa
     5581 catatccttg taacccgtgg atcactaatg aaaaaactgc ccactgcgga tatcctgaaa
     5641 tcaaatcgat caatagacca ctggaagcac caacaactaa tccacccgtt ggaccataaa
     5701 gaaaagcagc ggtatagatc ccggcctcgc ataatgtcac aaatccattc gtagccggta
     5761 cagggatcaa aattgttaat gaaagggcaa ctgtcaacgc aatcagcata gcatagatcg
     5821 ttaattgctt agttttcatt tctcattcct cctttttgaa taccgccaga ccagacactt
     5881 ccggtatgat cgttcatcag cacgccatta gaaatacaat gggcaacaaa ttttttactt
     5941 tctgctactg ctttggtaaa atctgcccct aacacaagat tcgctgttat tgcagaagaa
     6001 aaggcacaac cggccccgtt tactgttgta gtgtcgattt ttttatgaga aaatacctca
     6061 taatgatcaa ttgaatagag aagatccaca gcttcatctc cagccatcct tgtgccgcct
     6121 ttgatcacaa tgttttttgt tcctgaagca gtgagttctt tagcgagatg gatcatttct
     6181 gttaacgaag agggaagagc ttgaccagaa agtaaagcag cttctgccaa attaggcgta
     6241 atcatcgtaa caagtggaaa aagttcttcg atgagctgcg aaatatacaa ttgattgatc
     6301 acagcttcag tttctttgaa tgccaaaaca gggtctaaaa tgattgggat attttgtttt
     6361 gtttttatga agtctcgaac aaccatgatt gcttcacttg aattcaataa cccaattttg
     6421 ataccatcta atgaagtcat tttatcaatc gaaactaact gtcgtttaag caattccaaa
     6481 gatatagatt ccagcataaa tcgctcatta atatctagtg cccctataga agtcaaggcg
     6541 cttagtccga atagaccaaa attctcaaaa gtttttaaat ctgtttggat tcctccacca
     6601 gcaaatggat cagaaccgcc aattgtcaag attcttttca taacattttc ctccatgaat
     6661 aagagtataa cgaaattttc aaaaaagaaa aacagccaat tggcatattt ttatctcatc
     6721 caatttaaaa aaacaaaaag aaaagaaaga ccaaaatgga tctttctttc cttaaatagt
     6781 taggactttt gtgattttaa ttctttctcg atttgaagcc gttgcacttt cattgtagct
     6841 gttcgcggga ttgcatcaaa atcacggatg atagggacgt tcaagcgagg aagttcatgg
     6901 acttgctccc accatcgatt ccaatccatt tctttatcag gagctaaagc aataatcggt
     6961 tgtggagaat gatttttatc ccttacaatc actacttctg ccaaaaaatc taacgaatct
     7021 aataaaaagt cttcaatagc taatgtacta ttgatcgttt cgatcaaatc cacttgccga
     7081 tctttcaaaa ataggtggcc atgcttatcc ataaaaccgt aatctccgct atcccaccaa
     7141 tcgccataaa cattttcctg gaaccgtgca tcttccttgt aataagtcaa agcccgtcct
     7201 ttagacagta attgtatatg accatctgtg ttttcaggta ggacattccc aactgaatct
     7261 gtaatccgag cgcttgtcat gtcttccagt ccaacgccca tatctcttgc atcacttgtt
     7321 ttcaaactct ctagtgtatg agccttaaga atcattggtc cgcattcact ttggccatat
     7381 acttgaagaa ataccgcatc ctgtgccatc gacgttcgca aaaatgtctt cattgtctgg
     7441 ttgtttatag catcaaaagt ggagtgatag tatcggatac ccgaaaaagc ttctggatgc
     7501 tccttagcag taaaacgcca ctgaacaaag ttattcggat gggtctctag tgcaattggc
     7561 cggtgagctt ccaacatatg aacaactttt gaactttgtg cgtttgctaa aggcatcata
     7621 ggaaatccca tggacatcaa agaagagata ccaatattga atctggaatg cactggagag
     7681 atgtgaaaag cgattaattt cttttccgaa atttttgtga aaactgtttt ttgccatttc
     7741 gtccgccatc ccatagaatg agcagaatga caaataagtt tagggatacc agttgtacca
     7801 gaagtatggg tcatataaga aatttcattt ttgtctaaaa atgattgaga aacagacaaa
     7861 gcaggttgtt ccattaaata aagaatagga atctgttttg attttgttcc atttgaaatt
     7921 tctctcactc gcttttttgt ctcatcatca aataaaatga aagggtcttc taaacgatcg
     7981 ataaatactt ccattgttgt tgttggtaga tgataagaag tcatgacagg cactgcacca
     8041 aggtaagaca cagaaacagc tagaaggtat gtgtcaaaag cggaactctt ataaataata
     8101 actttgtctc cggatttcac tccaagatga gccaattgat aagcccgttt caacacactt
     8161 tcgcagcatt ttttgtaagt tgtttcaagt cctaattctg ggaaagctga aagtggctca
     8221 tcaaagataa tcggggtctc ggaaaagtta gctgcagctt cttgaaaatt cgtaaataag
     8281 ttcaaagggg aatacgttat ggtctccatc attcacactc cattatcttt tgttaagtaa
     8341 tatacaaatt caagataatc gtaacataat cctttatata ataacatagt ttattgtgaa
     8401 aaaaagatca tacatttgtt ttatatggaa gaaacccgaa cgaatgccgc taattcgtga
     8461 taagaaaaat caaccatctt ttcccagata tcttgaaaag aatgggatga gaggtatcga
     8521 ttaccacata aataatttac cgcgtcagca tcatctaaat atagttctgc ataataacga
     8581 tatgtttttc tgcatagttc tggcgatacg tttttatata ctgtaatttt gctcaaaatt
     8641 tggcgaatag tccgaggaga aatttgctga tcatgtgagg aacgaaaaac aaaagaattt
     8701 tcgtccgtat tttgtgctgt taacttaaaa tagttctcca gaaatggcat cgcttctttt
     8761 ggtattggta ctttattcag tctgttttct tttctattca gattaagaat ctgcttggtc
     8821 aaagatacat ctttacgttt aagttctact acttcatgga tctttaatcc gcaatacaga
     8881 agaatggcag taattgcaag gttcctcggt ctattctttt cttcaaagct agcttctctg
     8941 cttgtttgaa agccttcaat cgggataaac aaatgctcaa aatctttgaa aagttctgca
     9001 tcgatatatc gcggagctgc tttggttgtt tccggtaaac ttgtacccaa aagagaaatc
     9061 tcttcttcaa tcagtccttt ttgatacaaa aatcgtaaaa aatgatttaa tgaatattgc
     9121 ttccttgaaa tcccgtaata gctattttca aacaattgct ccgtttcttg gttcgctgta
     9181 gtttggaacg aacgagtatg tcgatagatt ttagctaaga aattctgata atcttgtttt
     9241 tctaaatgga taatcagctg atcatccact tcttcagaaa atacttttct caaataatca
     9301 gtaaacagat gaaaatcttt tgcgtattca tagcgactgc ctaatgaaag ggaagaacga
     9361 ttttcttcta aaaactgttg aaagacggaa gggagcaagt gtgtacgttg ctttaacttt
     9421 tcttgcagtt gttttctagt tttgctgtcc attacaaatt ccctccaata gtgttcattt
     9481 tctatatttt accgcatttc atcaaatgaa gatagacagt ttgtcttgat cttaggaagc
     9541 aaatttttaa ctaagtacta gctaaaagat atgaagtaag aagatacatt tttagttatt
     9601 ttattagtgc agtcattctt ttgatctttt aattgattta tcctcttttt agctatgtgg
     9661 ttacttcatg agcaatgaaa tggatttggt tcgttctaat ggcaggaggc tctttctttt
     9721 atttccgaaa aattagaaaa cattttttca gaatcgattg gctctccatt tattcaataa
     9781 tggtttgtct aacgatattg ttgggataat tcttaaagaa gtattagaaa tagttggtac
     9841 ttcattactt tacatgaaga tttattctat ggtaggagca attatattga gaattaatcc
     9901 ttttttatat aagttgtttg cttattgatg gattggataa attaaaccat cacaacgagt
     9961 ggaaattata tgaaattttt cataaaaatt catatagaaa caaatgacat tttattagaa
    10021 tatgactttc attaacataa atgttttcaa atttcaatta aattgtttta atcaaattcc
    10081 aatctaaata tttgtagaaa acaatcgtct cctatagaat aaatttataa gtaacaatat
    10141 accaatcata aatctatttg ctaggaggaa tttacatgga agagaaaaat ttaacggtca
    10201 tcggaagcta tagtacgagg gaagaagcat tatcagtaat tgaacggttg cgtaacgaag
    10261 ggtatgaacg agatgatatc gttatttata ctactgatga agcagcatct agactaggat
    10321 ttgacggatt gtctggtata gatgttgaaa cagatgaaaa tcggatggat ggtgaagaag
    10381 accgttcttt atgggaaaaa ataaaagata ctttttcttt tgatacctat gattctgaga
    10441 cggctacacc agaaaatgat cctttatatc aatatagagg agatatttct gatggaaaat
    10501 ttgtcatcac agtaaaaggc tatcgtcagc cagaaacaac agaagataca atggatacac
    10561 aagatactgt tagcccagca agcacaatgg ggactggatt agaaactggc actggaacag
    10621 atccaatgga cgtaggtggc acgacaggct ttcaaaatac aacggatcca ttgaatatga
    10681 caccaggaac tgatccgatg gacgtacctg gcgagactga tacaatgggt acaagcaaga
    10741 ctgaagacat cgatcgcaaa ccaaatctag atgatgatac aattcaacta aaagaagaaa
    10801 aattagatgt aaatacacat gatgtgacaa ctggagaagt cgacatccac aaacatgtgg
    10861 tcaacgatac tgaaacggta gaagtaccgg tcaaacgtga agaaatcgtc atcgaacgca
    10921 aaccagtaac agatcaatct tcacagggaa cagacgaaaa attggaagat gacacgatta
    10981 ctatcccaat caaagaagag caagtagatg taagcaaaca tacagttatt cgtgaagaag
    11041 ttgggattca taaagaagaa catgaagatg ttgaaaaagt gacagaagat gttagccgag
    11101 aagaattgga tattgacaca agcggtgatg tacacattga agaccgcaat aaaaaatcgt
    11161 aagcgtaagc taaagaggtt gtgacagaag tggtcagctt aaagaaattt cggcttattt
    11221 ccg
//
LOCUS       DBNICI010000097        10830 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.098, whole genome shotgun sequence.
ACCESSION   DBNICI010000097 DBNICI010000000
VERSION     DBNICI010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.098"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            190..693
                     /locus_tag="AC1ZCC_001962"
     CDS             190..693
                     /locus_tag="AC1ZCC_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HIQ1630207.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            753..1142
                     /locus_tag="AC1ZCC_001963"
     CDS             753..1142
                     /locus_tag="AC1ZCC_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HIQ1630208.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1129..2628
                     /locus_tag="AC1ZCC_001964"
                     /pseudo
     CDS             1129..2628
                     /locus_tag="AC1ZCC_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3376..5202
                     /gene="ltrA"
                     /locus_tag="AC1ZCC_001965"
     CDS             3376..5202
                     /gene="ltrA"
                     /locus_tag="AC1ZCC_001965"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HIQ1630209.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLVVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            5315..6256
                     /locus_tag="AC1ZCC_001966"
                     /pseudo
     CDS             5315..6256
                     /locus_tag="AC1ZCC_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            6261..8384
                     /locus_tag="AC1ZCC_001967"
     CDS             6261..8384
                     /locus_tag="AC1ZCC_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HIQ1630210.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            8381..9385
                     /locus_tag="AC1ZCC_001968"
     CDS             8381..9385
                     /locus_tag="AC1ZCC_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HIQ1630211.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            9404..10315
                     /locus_tag="AC1ZCC_001969"
     CDS             9404..10315
                     /locus_tag="AC1ZCC_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HIQ1630212.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            10502..10738
                     /locus_tag="AC1ZCC_001970"
                     /pseudo
     CDS             10502..10738
                     /locus_tag="AC1ZCC_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3567 a         1825 c         2404 g         3034 t
ORIGIN      
        1 caatccttag tcttactggc aattgtggtc attggtttgt ttttaggatt taagcgggag
       61 ttttccaaac tgattggctt tttggtgatt gctttgattg cggtcggatt ggtctttaat
      121 gcttctgggg tgaaggatgt cttactcagt ttgtttaacc gaattgtcgg tgcgtaaggg
      181 ggaagcacga tggaacagat gcgtgtttat attgcgaacc tcgggaaata taacgaaggt
      241 gaattggtgg gggcgtggtt taccccacct gtcgattttg atgaagtgaa agaacgaatt
      301 gggttaaatg acgaatacga ggaatatgca attcatgatt atgaacttcc ctttgagatt
      361 gatgagtata cgccaattga ggaaattaat cggttgtgtg gcttagcgga ggaacttgaa
      421 ggaacgccag ttggtgaagt tgcttctgag attcaacatg ctttcttcaa ttcttttgag
      481 gagatggtag agcatgtgga tgatattatc tattatccag attgcgatga tatgagtgat
      541 gtagcatatt tcttgattgt agaagctggt gattttggag aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtaga gacttggaga ttggaggcaa ttatttggta
      661 acgagtcgag gtgtttttga gtatccatat taacaaaatt attgaagggt agttgatttc
      721 taaactatcc ttctttttaa aggaatgatt tgatgaagaa aataaaaagc tacaccagta
      781 tttgggcagt tgaaaaggtc atttatgcaa ttaatgactt tcaactgcct tttcctgtga
      841 cttttaacca gatggcttgg tttgttttat ccttgctgtt tgtcatcgta tttgcccatg
      901 tgcctccact atctatgata gagggggcct ttttaaaata cttaggtata ccggtggcag
      961 ttacttggtt catgtcgcaa aaaacgtttg acggtaagaa accattggga tttttacgat
     1021 cttttattag ctatcactta cgatttaagg tcactttcgc aggaaagaaa gtcaaagaac
     1081 agaagaaacg atgggacgaa cccattactc tggtaaggag tgtgaactat gtacccgatt
     1141 aagtatattg aaaataatct tgtctttaat caagagggag aatgttttgc ctactatgag
     1201 ttaatacctt acaattactc ctttttgtca cctgaacaga aatatcaagt ccacgataac
     1261 tttcgtcaat tgattgcgca aaatcgtgaa ggcaagattc atgccttaca aattgccaca
     1321 gaaagtagta ttcgtgccac gcaagaacga tcaaaaaagg aaattaccgg gcgattaaca
     1381 gaagtagcga aacaacgaat tgatttacag acagaggcat tagtatccat gattggcgat
     1441 agtcagattg attatcgctt ttttattggc ttcaaactga ttgcgacaga tgaagaggtc
     1501 aatctaaaga atctcaagaa atctttcttc tccggctttc aagaatttgt ctatggagtg
     1561 aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg ccgatatacc
     1621 aagcttgaaa aactaatgga aagtaaattg gctcgaagat ttaaagtcag acgagtcaca
     1681 ccgagtgatc tgatgtattt gattgaacat atctatggcg aaaagggtac accatttgaa
     1741 gaatatgaat ttcagctacc aaagaaaaag ctgaagtcgg aaactttggt gaaacgttac
     1801 gacttattac gtcccagtcg ttgtttgatt gaagaaaagc cccggtactt atgcatggaa
     1861 catgagaatc atgaatcata tgtagcgtac ttaacaatta atacaattgt gggggaaatg
     1921 gagtttccct cctcagaact tttctattac cagcaacaac aatttacatt cccaattgac
     1981 actagtatga atgtggagat tgtgacgaac aaaaaggcac ttgctaccgt tcgcaataag
     2041 aaaaaggaat taaaagactt ggacaaccat gcctatcaat ctgacaatga aacaaattcc
     2101 aatgtcttag atgcattgga ttccgtagat gaattggaaa ctaccttgga tcagtccaaa
     2161 gaatccatgt acaaactgag ctatgtggtt cgagtaagtg ccgaatcagt agatgaacta
     2221 aagcgccggt gtgatgaagt gctagatttt tatgatgata ccaatgtaaa actagtccgg
     2281 ccttttggcg atatgatggg gctacatgaa gaattcttac cttctagtaa gcgatacatg
     2341 aatgattata tccagtatgt tacgtctgat tttctcgccg gacttggttt tggcgccact
     2401 cagatgttag gggagttgga aggcatttac tttggttata acgtagatac ggggcgcaat
     2461 gtttacctga aaccggcatt ggcctcacaa ggggtcaagg attcagttac caatgcgcta
     2521 gcagcggcat tcctcggttc actcggtggc ggaaagtcct ttagtaacaa tctcttagtt
     2581 tattacgccg tcctttttgg tggacaagct gtgattgtcg atccaaaagg gggtgcgaaa
     2641 cgttcctact tgaaaagagt aggcacagca cttcattaaa tcaatattta atgtgtgttg
     2701 gagaactaaa cacacgaata gtcgaatgac ggggtaacgc cctgaaaggc tactttaata
     2761 ctctgactgg cagaaacttg agtaaacaag gctctgtcag aagctcggtg aagtcggcag
     2821 acagacaccg taacagataa tgctgacgaa agattcccag aggtgggcgt tgtgtcctat
     2881 atgccggaag tctataaaat atctatggtt agaatgtaga tgagaggtct actgacgaat
     2941 ccttgaatgt acaggtctat atcggggctg gatagaaatg tccagtgaca caaacagtgt
     3001 cgctactgtg ggtgagtaag actccgcatt tatgaaaatc catataacgt taaaggcgtt
     3061 atcaaggtag caggcttata gaggaaacct aaggatatat gtacagatag tgtgtttgga
     3121 acgttgaaag ctgacaacat ggagatttta ctgtctgtga agtgttcaca tcagaaagtt
     3181 attgtgagat tagcaatgat ataaggtgtg ttaatgtcag tgagagtggt ggcacagtac
     3241 ctatgaaacc gtgataacaa gcggtggagg gatagccact agtcatatga agaatagaat
     3301 tactaagaaa aatggtatgg gttcgagtaa gactaagaga tgtacttgct cctgcaaaca
     3361 aggagggtac agaccatgct aaagaaaaac aagctccgct ataacgagta ttatgatatg
     3421 caacatattt atgacgagtt atacgcacag agcaagaacg gtaataactt ttataagtta
     3481 cttgaaatta ttggttcgga acaaaatatt tgtttagctt accgtaatct gaaatcaaac
     3541 agtggcagta aaacggctgg aacggatgga atgacgatag acgatataaa acaactcagc
     3601 aatgctgaga ttgttgctac cgttcgggaa agccttagca actaccgccc caaatcagtc
     3661 agacgtgttt tcattccaaa agctgggagc gataaaatgc gtccattggg gattccgtgt
     3721 atctgggaca ggctggtaca acaatgtatc ctgcaagtcc tagagccgat atgcgaacca
     3781 aagttccata atcattccta tggattccgt gcgaatcgaa gtgcccacca tgcagtcagc
     3841 agagtaacga cactgataaa tttatcaaaa tatcattact gtgttgacgt agacattaaa
     3901 ggatttttcg ataatgtcaa tcatggaaaa ctattaaaac aaatctggac acttggtatt
     3961 cgggataaaa gattgatttg cattatcagc aaaatgctga aagccgaaat tgacggcgag
     4021 ggtgtcccag aaaaaggaac gccacaaggc ggtctgttaa gtccactgct ctccctcgta
     4081 gtccttaatg aattagactg gtgggtaagc agtcagtggg aaacattcca gccgaagaac
     4141 cgcagtaaaa atgggtggct tcaatacgca aagaaatata ccaaactgaa aagcggtttt
     4201 attgtacgtt acgctgatga ttttaaaatc atgtgttcaa cctatggaga agcacaaaga
     4261 ttttaccaca gtaccgtaga ttttcttaat aaaaggctga aattagaaat cagtccagag
     4321 aaatcaaagg tagtaaatct gaagaaaaat tcatcagatt ttctggggtt caaaatcaaa
     4381 gtcattccaa aaggaagaac gaaacacggt tatgttgcca aaacagatat gaaccaaaaa
     4441 gcattaaaga aagcgaaaac aaatctaaag ttaaaggtaa aagatatagc cagacatacg
     4501 actggcttca atatcagcag atataatctg acagttatcg gtatgcagaa ttactactgc
     4561 atagcaacta acgtatataa caacctgaca gaagtaagtt acgctctctt accgaccata
     4621 cgaatccgtt taaggaacat tgcaaaatca gttccatttg aaagtacaag ttcagaattt
     4681 cagagcagga caaaaggaat cagaccaaaa acaaaaatag tgatgattgc ggataatccg
     4741 ctattgccga ttcaaggagt acaacacaaa aatcctatga atttctcgca agatatttgt
     4801 aattttacaa agcaagggag aaacaaggtg catgaggacg ttgtagttgt aacaaaagaa
     4861 gaaatccgag cgttgttgga aaatgaaaac cctgccgata gcgtagagtt taacgacaat
     4921 cgtatttcgg catatatcgc acaacaaggc aactgctatg taatgaatcg tcgaggaact
     4981 ccgtccactc tgatatgtat tcacaaatca gacaaaggag ataaccttga cagatattct
     5041 aatctggcat ttgtggaaat tcccattgca aaggcaatcc taacagaatc agcagacgaa
     5101 gcaaaatcac tgttaaaagg ttatgttctt aatagtcagc agaagaaaaa actgaacaga
     5161 atacggacga attacggata ccagacaata actgggaaat aacttataaa tctatttgga
     5221 tatgatggaa cgccgtatga agcgaaagtt tcatgtacgg tgtgaagcgg gggaaaactt
     5281 ggagataaca tcaaaaagtt acctatcgct ataacggggt ggctggaaag aaaccttgcc
     5341 agaaattgcg gatgaaatca atattctcaa cttaactagt aagccaaaaa atcaaggatt
     5401 acttgatcct tatgtgatta tggagaaaaa gaaggattct gaaagtctag caattgatat
     5461 tctgaccttt ttaacaggaa tttctagtcg agatggggag aagtttccag tgttgcgtaa
     5521 ggccattcgg acggttaccc aaagcaatca acggggatta ttacaagtaa ttaatgagct
     5581 tcgaaaagaa ggctcaccag ttgcggaaaa tatcgcggat cacatcgagt cctttaccga
     5641 ttatgatttt gcccatttgc ttttctcaga tggttcgatt acccagtcga ttagtttaga
     5701 taggcagtta aatatcattc aagtagcgga tttggtttta ccagatgcag aaaccacttt
     5761 tgaagaatat accaccatgg aattgcttag tgtttccatg ctaatcgtca tttccacttt
     5821 tgccttggac tttatccata gtaatcgaga aatcttcaaa attgtagact tagatgaggc
     5881 gtggagtttt cttcaagtag cgcaagggaa aacattatcg aataagctcg ttcgtgctgg
     5941 gcgggctatg aatgccgggg tttactttgt cacgcaaaat agtgatgatt tactggatga
     6001 acgcttgaag aataatatcg ggttgaagtt tgcgtttcgc agtaccgata ttcatgaaat
     6061 caagaaaacc ttggaattct ttggcttgga tcgagaagat gaaagcaatc aaaaacgcct
     6121 acgagatttg gaaaatggcc aatgcttgat gcaggatttg tatggtcggg taggcgtggt
     6181 tcaggtacat ccagtctttg aagaactctt ccatgccttt gatacccgtc cacctgttca
     6241 agagagaatg gagtgagggg atgaaaaaga aactttttcg gattgtggga atcgttttta
     6301 ttagcatact cactctgata gttctattga gtttagttgg tacggtagcc gaggcgacag
     6361 ggctagttga tgatacagtt gaagcaggaa atctatattc caaatattca ctgaacaact
     6421 atcaactaga tttctttgtg gatagctctt gggattggtt gccttggaat tggggtgatg
     6481 gcttagggaa aagcgtgatg tatggtcttt atgccattac taatttcatt tggacggtga
     6541 gtttgtattt gtcgaacgcc acgggttatg tggttcaaga ggcttacaaa ttagatttca
     6601 tttcggatac ggctgaaagt attggaaaga atatccaaac attggctggt attactgaaa
     6661 atggactcca gacctccgga ttttattttg ggtttctatt attgatgatt ttagctctgg
     6721 gggtgtatgt ggcgtatacc ggattattaa aacgagaaac cacgaaagca gttcgagccg
     6781 tgattaattt tgtgatgatt tttctactat cgggttcgtt cattgcttat gcgcctacct
     6841 atatcacaaa aatcaatgac tttagctcag atgttagtga agcggccctt acccttggaa
     6901 ccgagatcgt tgtacccaat tcagaaagcc aaggaaaaga tagtgtggat ttgattcggg
     6961 atagtttgtt ttcgattcaa gtccaacaac cttggttact actgcaattt gatgattcca
     7021 acatagaaga aattggtgaa gaccgggtca acaagattct atctgtgagt ccagatgaaa
     7081 acaaaggcaa agatcgggaa gaagcggtca aggcagagat tgaagacaat gacaatgcga
     7141 atctcagtat taccaagacc atgaaccgtt tagggaccgt ggtctttttg gtattgttca
     7201 atattggcat ttccttcttt gtttttcttc taacagggat tatgttgttc tcgcaaattc
     7261 tcttcattat ctttgcgatg tttctgccaa ttagtttctt attgtccatg ttgccgactt
     7321 atgaaagttt aggaaagaag gcgattattc gtttattcaa tacgattatg atgcgagcgg
     7381 gtgttacttt ggtgattacg actgcattta gcatctccac gatgtttttc aatatttcgg
     7441 caacctatcc atttttcatg gttgcttttc tgcaaattgt gacctttgcc ggtatctatt
     7501 tcaaattggg cgacattatg agtatgttca acctccagag taatgatagc cagtcaatgg
     7561 gaagacgcgt catgcgaaaa ccgcaaatgt taatgaatcg aaaattgcgg caactcaatc
     7621 gaaatgtggg acgagctttg gcttttggtg gtggtgcagc agtcggaaat aagttggcaa
     7681 aggaacaatc aaaatccaaa tttaagccat ccggttcttc tctacgaaag aattcacgat
     7741 taccgaacga tcacgaaata ctatctgact taactaagga gaatccagtt tcaaataata
     7801 agaaacaatc ccgaatgaat ttggctggga gaaaaatcgg caaagttttg gatacccaag
     7861 ctttggtgaa agataagact aaacaagtaa aggatcaggt aaggaatacg ccaacgaatt
     7921 tgaaatacaa tctccataaa ggaatcgaaa agtcgaaaaa agcaccagaa gaattcaagc
     7981 gcggactgtt ccaagaaaaa gcggatcgag tggatttgcg agaaaaacaa cgacaacgaa
     8041 gaaatgcgaa aatggacgaa aaacgcaaga tactgagtga agcaacaaat cgtcagggaa
     8101 aaagaagggc gaatattcca atagataaag cagctcaacc tcagaaagat atgatgctga
     8161 aaaaagaagc accaaagcgg attgtgagag caaatctgaa tcccgaaatc aaacgaaaac
     8221 taactagtca agaaatcatt tcaaaaggga aaagccaatc agcaagtaag aaaaattctt
     8281 tccaacaagt gaaacagagg tccactctgc caaagcagac caatcaaaaa gtacaaaaaa
     8341 gaatgaacca tcgtccaaaa tcaaggtcag gtgagaaaaa atgaacttga agaaagtggg
     8401 tgttatttca ttaacttttc tgaccatctc ttttatgggt attttgatgt gtgtgggttt
     8461 gttgtttggt gaagacagtg agggaaatgg tagttctggc acttctcctg gcggaactag
     8521 tgtctcagaa gaagtattga aacaccgtgc aatggtggaa aagtatgcca aagaatcgga
     8581 tataacagag tatattccaa ttcttttggc gattattcaa gtggaatctg gtggcacaat
     8641 ggaagacgtt atgcaaagtt ctgagtcagc cggattacca cctaattcat taagtaccga
     8701 ggcatccatc aaacaaggct gtctctattt tgcgtctttg gttaagcgtt ccaaagaact
     8761 agggtgtgat caagatagta ttattcaggc ctataactat ggtggcgggt atctagatca
     8821 tgtcgggaaa aacgggaaga agcatagttt tgctttagct gaatcttttt caaaggaaaa
     8881 atcaggtggt caaaaagtcg attaccccaa cccaatcgcg attaaggaaa atggtgggtg
     8941 gcggtataat tatggcaata tgttttactg tttattagtg aagcaatacc taactacaac
     9001 tcagtttgat gacaaaatag tccaagaaat ttttgatgag gcatttaagt atgaaggaac
     9061 ggcctatgtg tttggtggct caagtccaga aacggggttt gattgtagtg gcttgaccca
     9121 atggtgttat gccaaagttg acctgaaact tcctcgggtg gctcaggatc aatatgatgc
     9181 catgactcat attgatctaa aagatgccaa accaggtgat ttagttttct ttcattcaac
     9241 ctatgatgcc ggtacttatg tgacccatgt gggcatttac gcaggggaga atcgcatgtt
     9301 tcatgcagga gatccagtgg ggtggacgaa tctaacggaa aactattggc ggcagcatct
     9361 tatcggagct ggccgataca aataagagaa aagaggaaaa agaatgaaga taaaaattga
     9421 acggaatcaa aaggaaaaga gcccaaaaaa gaaaaaagaa cgagttgtat ccgtgggtaa
     9481 gcatcgaaaa atggtgcttg ccctttggct tttattgtct tgtagtcttg cttttggcgt
     9541 ttataaaaat ttcaccgcca ttgatcaaca tacaactcat gaaaaagtgg ttataaaaga
     9601 gaaggtagtg aatacttctg gcgttgaaag ttttacagag gattttgcga aagaatattt
     9661 ttcatggaaa aataacaaag aagcaattga aaagagaatg agtaatctcg aacagtacct
     9721 tacagaaaac ggtctcgctt taatccaaga tatggttcga gcagatattc ccacgagttc
     9781 cgaagttcaa tctgtaaaaa ttctagatgt tgaaaaacac tcggaagaat ttgtcgtttc
     9841 atttttagtg gagcaacata ttacagaggg aaaaaagacg caatcaattt cttctgcata
     9901 tcgagtgaca atctttgaag atgaaaacag gaatcattta gtaactagtt tgccaacaat
     9961 gattggaaag ccagacaagg caaaatatga agcaaaacaa gtagaaaacg attcagaaat
    10021 tgatgccaaa acaacggaag aaatcaccga ttttttagaa acatttttta aactttatcc
    10081 atcagcttct gaaaaagaat tggagtatta tgttgaaaat ggtgcgatgc gaccaatcaa
    10141 tattgactta aagttttcgg agttagagaa tccagtttat ctacaaaacg gaaaaaattt
    10201 tctagtgagt acaaagataa aataccttga taatgtatca aaaaccacta gctactttca
    10261 gtacaaatta gtattacaaa aaaatggaaa ctggaaaatc gttgatgtta aataataatg
    10321 actcaatcta attttattag aagttataag tagtgtaact tatttcaggc agaagtcgcc
    10381 catcctctat aggtgggtga tgaatgccat tcggtaactc gaaaaccgac aattaagaca
    10441 tagtgccttt ttattttaat ccatgtcata atcagtctat ataggaaatg gttgatatac
    10501 catggaatta gatacaaata atcattcagt ttttcttctt ggttaccccc ttattttagt
    10561 agtgaaacat tgtaaacatg tcattgatga tgtaatgtca gcttatgcaa aaactgcctt
    10621 tgaaagaatt tcagaatcac atcacattac cttagatgaa tgaaatcgtg ataaagaata
    10681 ctttcatatt atgctcaaag cacaacctaa aaccgaatta acaaaattca gcaatgccta
    10741 agctcacgtc tgataaaatg agactttcta agggtcaaaa atttcctttg aatacgatta
    10801 ttgcttagat aaaagtaaaa gccttagaac
//
LOCUS       DBNICI010000098        10695 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.099, whole genome shotgun sequence.
ACCESSION   DBNICI010000098 DBNICI010000000
VERSION     DBNICI010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.099"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            297..1850
                     /locus_tag="AC1ZCC_001971"
     CDS             297..1850
                     /locus_tag="AC1ZCC_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIQ1630213.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(2231..4015)
                     /locus_tag="AC1ZCC_001972"
     CDS             complement(2231..4015)
                     /locus_tag="AC1ZCC_001972"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1630214.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(4030..5751)
                     /locus_tag="AC1ZCC_001973"
     CDS             complement(4030..5751)
                     /locus_tag="AC1ZCC_001973"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1630215.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            5851..6612
                     /locus_tag="AC1ZCC_001974"
     CDS             5851..6612
                     /locus_tag="AC1ZCC_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIQ1630216.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            7554..7847
                     /locus_tag="AC1ZCC_001975"
                     /pseudo
     CDS             7554..7847
                     /locus_tag="AC1ZCC_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            8519..>10695
                     /locus_tag="AC1ZCC_001976"
     CDS             8519..>10695
                     /locus_tag="AC1ZCC_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HIQ1630217.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
BASE COUNT         3478 a         1803 c         2142 g         3272 t
ORIGIN      
        1 cagaatgaac ttcattctgt ctcagatttt ttgttatttc aaattgatta attgattgat
       61 caaaaaggtt ttgattttca tgttctcaaa cacgttcaat gtctttttct tctgaatggt
      121 cttgtaagag atctctgtaa tcatcaaatt tttgtctatt aatcttagta ttttttgatg
      181 tataaactac ctgaaattga gagtacatac caaattcata aattttttat tagtgggact
      241 agtgattgta tggattttga cgcataatga atgaagtatg agaaaaggag agacaaatgg
      301 atcaagaact atttattatc gaagtattat cagctaaaca aaaaattatc gagaaagcga
      361 aaaaaagagc gaaaagaata atgttcaagc ttttaagctt agtatttttc tctggtttgc
      421 tttttttcgg agctgtatcg ttcctatatc gccatatgag tgcagtagtc cgttcagctg
      481 aagggatgca aaaattaaat aatttgtatc acttttcgtg gggagttggc ggaataacaa
      541 ttgttgtgct tattgttttt cttctactta tttggctccg ttaccatcaa ttgtcgcgtc
      601 atttcgatca atggcttagt caaagagcac aacagttttt acataataaa ccaacttctc
      661 aagatgatga ctatctttat tttgagaaga cttcgacgag tcaagctttt cggatacaaa
      721 aaagtgattg ccagttattg ctgagatcac ttgatgattt acctatctac ttaggcgaaa
      781 gcaaatcaaa attcggaatg aacacattcc atattttttt actagaaaac gaacccagat
      841 gggaaaaaag ccagtcttcg cctggtggat acctgacaac taagatgagt acattagtca
      901 ctggtttttt cctcattctt ttagctggaa caatctatta tgggagatac actcagccaa
      961 aaatgtcttc tcatataaat gctgcttctc acatgacaat gaactcaact aacgatacgc
     1021 atatacaaaa gacaaatgaa gggttgttag ctgatcctaa gcaaaaggta aatttgacaa
     1081 cacaaaagat aaatgatctc aaacttgata cagagacgca ctctctctat atgacaacag
     1141 atagtggtgc taattgggta tttgtgccga ttgattcgca atggttgcgt tttggcgaat
     1201 acaccctgac aacggggctg attcctgaag gttattggat ggacaacaca tatgatattt
     1261 ccgccgattt ctcatggttt atctattctt ctggggatga tgtacgtctg ctgacttcaa
     1321 gagataatgg taaaacatgg caaacgtcta ttatcaccca atcagttcag ccgatacgtt
     1381 atcgaaaagc ggtattttat ggcaacgatg gtctatttat ctgtacatca gccagtggga
     1441 tgtctgcaga aactttgtac atttacacta ctatggatgg cggaatcact tggataaaaa
     1501 gtggaagtac aatgatcaat cagccagtac aaaatgtcag cttcgttaca acttctttag
     1561 gttttgtttc gacacgtgaa agtttgtttt atacaaacaa cggaggacgc tcattcaaag
     1621 aatcagttct gtcgattccg gaaaactatc agacaggggg gatagatctt tttcaatcac
     1681 caaatgaagt tactcaaata agcgcgaata cgttagagac aaaattttat ttgttgaaaa
     1741 aaggaccaat cgatgcaggg aaaatgtttg cctgcctctt ccgttcaact gataacgggg
     1801 aaacatggca attcgttcag caactatcgc aagtagaaca aggagattaa agaagagaga
     1861 ttgtgagcct gacgttcagc accgagtcac aagaaacaaa ttcaaaaaac agcttctcat
     1921 atttgttgaa tagtgtaact cgtgattcgg agttgacaag agcaacaaga agcgattttg
     1981 aagtatagct tctcgtattt tacaagatcg tgtcttattg ccgataggtt gtctcttgaa
     2041 caccgtttat tcgtaaaaag ggattgtgac aagtcttttg tcactactta ccgaacgaaa
     2101 ctgaaggagc tatccaaaaa gtatcattag tattattctt tgtgtttcta gtggcaccac
     2161 tagtacattt aaaaaagcta tcgatcatgg actaatcata catgatcgat agctttttag
     2221 ataatgtttg ttacgctatc tgaaattgac tattataaag actggcataa agcgttggtg
     2281 attggagtag ttcagaatgt gttccttttt cgacgatatc gccattcttc attacaagga
     2341 tcatgtctgc attttcaatc gttgacaaac ggtgtgcaat cacaaaactt gtcctatctt
     2401 ttgtcacagc ttccattgct tgctgaatca atgcttctgt tcgtgtatct acgctagaag
     2461 ttgcttcatc taaaataacg acaggaggat tcgctagaat gactcgagca attgtcaata
     2521 attgttgctg tccttgagat aacgtgccgt tttcgctaga aatgatcgta tcatatccgt
     2581 ctggcagtgt tcgaataaag tgatcacatt gtgcgatttc agccgcttcg ataatttgtt
     2641 ctcttgtggc atccattttc ccgtaggcga tattatcagc taccgttcct tcaaacagcc
     2701 aggtattttg taaaaccatc ccaaaagaac gacgtagttc tccacgtgaa agcttggtaa
     2761 tatcaagacc gtcgaattcg atctttcctt tattcagttc ataaaaacgc atcagcagat
     2821 tgactaaagt cgtttttcct gcaccagttg gaccaacgat cgctaccgtt tgttttggct
     2881 ttactgaaaa atcgacttga ttcatcaata ttttatctga tgtgtaacca aactgtacat
     2941 ttttgaaagc tatttttcct ttcggttgtt ctaaaagtct tgcatctgga aggtctggaa
     3001 tttcatctgt ttcatctaat atctcaaata tcctatcgat tgctgccatt gcggattgga
     3061 tcgagttgat cacataagaa gcggtagaaa ttggttcaga aatctgattg atgtactgaa
     3121 gataagcttg taagaagccg atcgtcacac cacctgatag aacaagcata gcaccgaata
     3181 ccgcacttat gatgaaggcg agttgattaa tgaaacgaat ggccggatag atagcacagt
     3241 tcataaattg agcacgttta aaagcgttgt attgcttttt atttacttcg tgtatagcag
     3301 cttctgcatg agcttgctga ttgaatgttt tgaccactag attaccagct aagatttctt
     3361 ccattcgatt atttaaaaca cccagttcat actgattctg ttcagaaatc tgtttgtttt
     3421 tgttcgcaat cttagttgta acaattgaac tgccaacgat cagcaataag acgagcaaag
     3481 taagcttgct ttcgatataa aacagcatca gaccagcagc agctattgtt acgacagaag
     3541 taaaaaattg attgatacct gtcaataaca cttgagaaag ttgattgaga ccggtagttg
     3601 tccgactaat gatgtcacct acttgatgat tgtcaaaaaa cgacatcggt aatgttttga
     3661 atttttttgt gatttctttt cgcaccctca atgtgatctt ttcgcttaac gctgccattg
     3721 tccgttcctg aataaatgaa gtgatgctgc ttaagactga acagccgatc agcaataaga
     3781 cagggaacaa caatgcttgt ttgatatcac tgaaagaaag ctgtccaata cctgtttttc
     3841 ttatttctga taagagatca tcgatcccga ttcccatgat caagggcatg gctacgacta
     3901 ataggttccc gaataaactg catagaaaca atccgtaaaa atatttatgt tcaggtttga
     3961 tcattttcca aaaacgtatc aaagcacgcg tgttcaacgt ttttttcttt tccatcgtat
     4021 cccttctttc tatattcctt gtgattttgc aaactcctga taagtttggc aattagacag
     4081 tagttcttca tgagtacctt gtccaacgat ttttccttca tctaaaacaa tgatgttatc
     4141 tgcttgttgg attgtactga ggcgttgagc aacaatcacg agagttttct ccgacatgtg
     4201 tttatgaagg gctgctctta atgctgcatc tgttttatag tctaaagcgg agaaactatc
     4261 atcgaataca tagatctctg ctggtttgat caacgctcga gcaatacata tccgctgttt
     4321 ctggccccct gaataattag aaccgccttg agcaacaaag ctgtccaatc cgtcaggaag
     4381 ggtgtccact agttcttcta attgagcgat cttgatcgct ttagacaact cttctgttga
     4441 ggcatattca tttcccatct gcaagttgct taaaattgtc ccgctgaata agaaagcctt
     4501 ctgagggaca taactgatgt gggagcggat ctgttcttga gtcatttgag taagaggaat
     4561 accaccaagg gttacttttc ctatcgtagg gtcactaagt ctcaagagaa gtttcgccaa
     4621 actacttttt ccagatccgg tcccgccaac gatcgctgtt gtttttcctt taggtattgt
     4681 gaaatcaata tcttctagaa ctggatcaga gatttcagta tatttaaatg tgacatgttc
     4741 aaaagcagct gcatttgtct gatcaaaggt tggttcagct actgttttga tcgtctcttc
     4801 ctcaatttcg atctctgctt gcagtatttc ttctatccgt cgtaatgagg caaacgattt
     4861 agggataatg accaatacca tagctgcgat catcaagtaa gcaagtgtca gcatagagta
     4921 ttcgactaca gcagtcacga caccgatttg taatgctcct tctgctacaa gatttccgcc
     4981 aaaccacaaa atagaagcat aaacgattcc catgagcaaa aaagcaattg gagtgaccca
     5041 agcaaatatt cggttcactt tgatcatttg attagcatag tcagcaaagc tcttattcgt
     5101 tcgcttttct tcatatgatt gattatcaaa ggcacggatc atattaatcc cagtaaagaa
     5161 ttctctcaat gtgatggtga tccgatccat ttttggctgg atactcaaag ataaaggtgc
     5221 ccctcgtttc atcagtcgat aaacaatcca tacataaaaa acaacagaga tcaatgtaac
     5281 ccatgttagt aatagagaat aacgaaacat catgagaatg gtgaaaacgc aaataaccgg
     5341 tgctggtaag atcatttggc aaaataaaac aatcatttgc tggacattat cgacatcatt
     5401 cgtcattctt gtcaaaagag atccagaact aaaacgactg atatccttca ttgacagctg
     5461 ttggaattta cggaaatatt tctcccgcgt ttggaatcca aacctagcgg aagccaatgc
     5521 tgaaaagtaa ctagaggcaa ctgcagcgat tgttcctaga aacgctacaa gcagcatttc
     5581 aactccaatt ttttttataa ctgaggcttc gccacttgct atacctgtgt cgatcagctt
     5641 tgctactaat tttggtacac ctaatgtacc ggcgatttct aaacagatca tcacaaaggt
     5701 aaggagagcg agtcctttgt ttttctgtaa gaatgttttt aagatagaca tattattcct
     5761 ccttttttcc tgatactttt cgattctata ccttcaagta acttgaaggt caatagaaaa
     5821 aagaataaac tgaaaaagag gtgagaaaaa atgaaaaaaa tgtatacgat tggccaagta
     5881 tcggaactat ttgatttgcc aaaatcaacg atccgctatt gggatgaaca aggattaatc
     5941 cgttcaagta gacaagaaga gaatgattat cgattgtttg atatcgatga tatttttatg
     6001 ctttatgata tcgtttttta tagaaaatta gatataccga tcaaacagat gaagaattta
     6061 tatggaaaaa cattgacgga gttatatgag acacttgatg aaacagaaag aagaatccat
     6121 aaagaactgg tggtgatgaa acaaaaacaa aaagagataa gggagcaaaa aaaacagttg
     6181 aaattgatga tcgatacaaa cgaggaagaa tttcctgtag aagagatccc ttttgactgt
     6241 atgatctcaa cggagtttga agatattgtg gaaatcaaaa aatttctacc taactattca
     6301 agctttggga tgatgagtat gccagcttcc agttcacaga cgatgtatgg attttttatc
     6361 gaccctagtg aagtacattt atttactcaa gacgtgattt gggagaaaaa agatacggct
     6421 gtttatcgtc gattcttatt gaaatccgag atgaaccatg cggaacgtaa taatatttta
     6481 gaaatcagag aaagaatgta tgaaaaagga tggaaaacag gagaagtgat cggccagtat
     6541 ctattgacga atacggatga aaacaatatc agaactgaat attatcatgc atggatagaa
     6601 atgaaaaaat aggaaatatg tgtttaagat aactcgaaaa tgatggttgt tttagttata
     6661 aagaaaaatt ttctggttcg attacgggtt taatgtgggg gattattagt cgtggataga
     6721 tagaatttta gttttttaga ggaaatatgt ctttaaaaat ggaatggttg caattcttag
     6781 cgatatatac gatgtaattg tcctcttaaa aaagtatgga aaaataattg tattgggtgt
     6841 tatgtataat tcatggtgaa ctgagactaa tgagaagaaa aattcatatt gttatgagtg
     6901 agattgtatc agatgagaat gataaattaa ataggtgcat aaaagcatta cacttaaaat
     6961 acattgttgc ttacattatt acaatatgtt aataaggcaa aaataaatag atggattgaa
     7021 taaacatatt ttttaatcaa atagtcccta ctgtgacttt cctcagctat cggtgatact
     7081 accgatagca tttttttgct tataaaataa ttataagtat ttagagattt ttcttctaac
     7141 attcttcaaa caagaaaaat cacaaaggtt attgttattt ttatatattc tttttaagaa
     7201 tgattgtttt tttatagaaa ttttaaaaaa taaaacacgt tattgatgga tagttagttt
     7261 gttatttggt aatattatag aagttgactg gtagtgattt atttttcgtc gttttataaa
     7321 aatgttattt tttggaaaaa ttcacaaata tgtaacgaat gtttttctgg aatttgctgt
     7381 gttaaaaagc agtatgacta atataaatac tattgtcatt tatgcaattt tttatggata
     7441 gtaattttag aggggaatca atgaaggaag aatagaaggg aaaaatagtt gcaaatacgc
     7501 cagttagcaa aaatagcaat acatcttcaa gctttaaaat tcgagagaat catcttggta
     7561 gtggaatcaa ctacaacaaa aggattaaac caattaatag atagaattac caatggaaaa
     7621 atagaaaagg tagttattct atacaaaagt tgtttaattc gcttcggtta tgaacttagt
     7681 gaaacccttt gcaatatatg gtacagaata tagaaattat tgacattaca gaaaatgatg
     7741 aagatttaat acagattgtc actatttgta gatacctttt acaaggaaac cgagccaata
     7801 aagctaagaa gatgattaag gagttgatgg aagacgatac aagttaagaa agtgaaacta
     7861 cgcccaaata aagagcaaaa agagtaattg gacgtggaaa atgcaagaaa tgaaccatcg
     7921 attcggtgga cagtttatta gtaatgacgg tttgagaaca tccatgacga gaatgaagga
     7981 acatcctaaa tgcgtttagt tgaaccaagt gccaaataat atggccaaat aagcgatcag
     8041 ttatcgatga atcccaagta ttctaaacta tatattcatt ttgatgctaa gcattgatat
     8101 atttttttgg gattgatgaa ggttaaccaa tacagggact cactgatgaa attctagagt
     8161 ctgaccttgg tatcaacgat ttagtgattg tttctaatgg aaaagtataa aagtatataa
     8221 gtacattaac aaaataacta ttagggttac gctccagctt gtgcaaggca ctggcaggag
     8281 gaatcgtgaa atcataatta tggttaagcc acacggacaa ctaaaagtca tacaaagcta
     8341 tcgaatcaat caacagaaga caatcggcaa tcattagctt tgtttacagc ttacattgca
     8401 acgcttgagt tgaaccgaaa atacgaggga ggtaacgcag ccattttgag ttggggaatc
     8461 tttttagaaa attatcgggt accttgttgt ttagtcaaaa ttataagggg gagtaatagt
     8521 gaggaacaaa tttcataaac tgttaagtat gtttctagtt atgcttattg tgatcaatca
     8581 atttgggaac ataacggttc tagctgagtc tttagcagaa cagccagagc gttcgctgac
     8641 gatcgaagtt gatcaagagg aggctgcttt gggtgagcag ttccagttgc gagtcagagg
     8701 aacaccagac ctgctaaatg cattggtcat tgataagagt gaggatttga ttgaagggga
     8761 agtgaagcaa ctcaatgatt acgaacgtct gattacttat tcttcccagc attcaggtaa
     8821 atacgaattg accgcaagaa gcggtgatat tcaatcaaat acggtctcaa tagaaatcag
     8881 tgtaccagaa gaaagcacaa gtgagtcttt taatgtacca gaagaaagca caagtgaatc
     8941 ttttaatata ccagaagaaa gcttaagtga atcttttaat gaagccagat catctgagcc
     9001 atctataacg caggaatctc agattgaaag acaagaggag ctcttgaatt atgacatcgg
     9061 catctctgct gagcgcccca ctttgccgag caggcataca caaacgttaa agggatacat
     9121 tcaaggaggc gctggcgata ttcttgaaga atatggggag atggtcgtac gagttcctag
     9181 agcatcgatt ggcttaattg agttcattag agtggaggtc gctgatgatt cacctttaaa
     9241 gccactggca ttttaccaag aaggcacaga cgttgttttt ccatttacct ttgattatga
     9301 aggattaaat gaagccgctc agtcgtatac attcagtatc acgtttgaag ttgctttctt
     9361 tcaatcaggc ggtactgagc caaataatga ctttcaagtg agtcttactt atgctgagcg
     9421 agcagcaaac acggaatttt caattacgcc tattccttca tcaagaccca ttttttcgaa
     9481 attatactat ggctctagag aaatcgacag ccaaggaaat cttgggaatc tgctgattga
     9541 cgggcaagac gtgaccaaca gtaataaatt tattttgcag gtaaattatg aaggatcacg
     9601 aaaagacggt gtagtggtta ccgatgtact accgcaagga acaactttgg caccgaatcc
     9661 gactacatgg agttccactg gaaatagaga acctatttca ggtggggttc ggatcgcaga
     9721 agtaacgaat ccaggtgaag caaacgttag aactcattat gtttccgagg aatttgtgga
     9781 caaagtatct tttaataagg aaacgaatac acttgttgtg gactttggta atctagcaga
     9841 gggtcaatcg tattttattg aatatgctct tgaagtgacc gatgtcgact tgttcaatga
     9901 aggaagacaa gaggatgggt tttctcggaa tcgagcgagt atgacctaca ataatcaggt
     9961 aatgagccga gactaccgct tgagaatccc tgaaagtaat cttccggcaa tctctttaag
    10021 aaaaagtgtg gacaaaaatg ttatcaatct caatgaaaat gaactaacgt atactctgca
    10081 tgtctcgatg ttttctggaa caagaaatac gcttgttatt gaagatgttt tggaagaagg
    10141 catggaattt gtgtcatttc tatcagatat tccctctttc attgatgccg aagtcaataa
    10201 tggaatagtt cgtttcagtg cgaataatac gattggtggt ccgacaagtt ttaccctctc
    10261 ttttaaggtc aatgtagagg cgtatgcggt cggcgatcaa attttaaact ttgcaacgct
    10321 tgtgaatagt gacaatcgat tgaatacgag tactgtaaga acaaggaaaa tcgatgggcg
    10381 tattaaagta attaaaactg atgagaatgg ccaaaggctg gcaggagcaa aatttgaaat
    10441 tttatcaagc gattcacaag tgatacaaga aggtataacg gcagaaaacg gagaattttt
    10501 aagtgaaccg cttcctgtag gtagctatca agtccgagaa acagaagcac cggaagggta
    10561 tcttttagat gcgacggtac atgatgtttt tataaatgaa tactccatag aagaagtgac
    10621 gttaaccatt gaaaacatcc ttgatacggg tggtgttgaa ctaattaaac atgctgaagg
    10681 cagtggtgaa gtcct
//
LOCUS       DBNICI010000099        10667 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.100, whole genome shotgun sequence.
ACCESSION   DBNICI010000099 DBNICI010000000
VERSION     DBNICI010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.100"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..328
                     /gene="pheS"
                     /locus_tag="AC1ZCC_001977"
     CDS             <1..328
                     /gene="pheS"
                     /locus_tag="AC1ZCC_001977"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="catalyzes a two-step reaction, first charging a
                     phenylalanine molecule by linking its carboxyl group to
                     the alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; forms a heterotetramer of
                     alpha(2)beta(2); binds two magnesium ions per tetramer;
                     type 1 subfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIQ1630218.1"
                     /translation="KMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGCNVCKYTG
                     WIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFYQNDLRFL
                     SQFKGE"
     gene            333..2753
                     /gene="pheT"
                     /locus_tag="AC1ZCC_001978"
     CDS             333..2753
                     /gene="pheT"
                     /locus_tag="AC1ZCC_001978"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIQ1630219.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(2923..3933)
                     /gene="trpS"
                     /locus_tag="AC1ZCC_001979"
     CDS             complement(2923..3933)
                     /gene="trpS"
                     /locus_tag="AC1ZCC_001979"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIQ1630220.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            4187..4924
                     /locus_tag="AC1ZCC_001980"
     CDS             4187..4924
                     /locus_tag="AC1ZCC_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIQ1630221.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            4938..5753
                     /locus_tag="AC1ZCC_001981"
     CDS             4938..5753
                     /locus_tag="AC1ZCC_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIQ1630222.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            5766..6413
                     /locus_tag="AC1ZCC_001982"
     CDS             5766..6413
                     /locus_tag="AC1ZCC_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIQ1630223.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            6429..7085
                     /locus_tag="AC1ZCC_001983"
     CDS             6429..7085
                     /locus_tag="AC1ZCC_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIQ1630224.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            7247..8068
                     /gene="racE"
                     /locus_tag="AC1ZCC_001984"
     CDS             7247..8068
                     /gene="racE"
                     /locus_tag="AC1ZCC_001984"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HIQ1630225.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            8071..9426
                     /gene="rph"
                     /locus_tag="AC1ZCC_001985"
     CDS             8071..9426
                     /gene="rph"
                     /locus_tag="AC1ZCC_001985"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HIQ1630226.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKEQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            9427..9945
                     /locus_tag="AC1ZCC_001986"
     CDS             9427..9945
                     /locus_tag="AC1ZCC_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIQ1630227.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            10052..10552
                     /gene="cbpB"
                     /locus_tag="AC1ZCC_001987"
     CDS             10052..10552
                     /gene="cbpB"
                     /locus_tag="AC1ZCC_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HIQ1630228.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         3485 a         1837 c         2312 g         3033 t
ORIGIN      
        1 aaaaatgttt ggggaagatc gtgaaattcg tttgcgtcca agttatttcc catttacgga
       61 accatcagta gaagtagatg tcagctgttt caaatgtggt ggtgccggtt gtaacgtatg
      121 taaatacact ggatggatcg aaattttagg agctggcatg gtacacccga atgtgttgaa
      181 gatgtcagga atcgatccag aagaatattc aggctttgct ttcggccttg gaccagaccg
      241 cgtagctatg ttgcggtatg gagtgaatga tatccgtaat ttctatcaaa atgacttacg
      301 tttcttaagt cagttcaagg gggagtaaga aaatgttagt ttcatataaa tggctcaatc
      361 gttatgtaga tttatcgaat gtaacaccaa aagaattagc ggataagatg tctgtgacag
      421 gaatcgaagt agagggagta acggttccag aagaaggctt gaaaaaaatc gttgtcggtg
      481 aagtcaagga atgcgtacca catccagatt ctgatcacct ttctatttgt caagtcgata
      541 ttggcgaaga cgaactgtct caaatcgttt gtggagcgcc taatgtgaag gttggaatca
      601 aagtgattgt tgcacttcct aactcacgta tcgctggtaa tgtaaaaatc aaaaaaggaa
      661 aaatgcgtgg ccaagtatca aatggcatga tttgctcatt acaagaaatt ggctattcag
      721 atagtgttat accgaaagaa tacgctgaag gtatctatta tatgccacaa gaagctgtaa
      781 atggcgatcc agtttttcct tatttggata tggatgattc gattattgaa ttatctatca
      841 caccgaatcg tgcggatgca ttgtcaatgc gtggcgtagc ttatgaagtt ggggcaattt
      901 atcgtcagac tcctacattc gatgacgaaa agctggtaga atcctcacgt cctgctagcg
      961 ataaaattca agtggaagtt gaagataaag aagcagttcc tgcttatcaa atcagaatca
     1021 ttgaaggagt aagaatccag ccaagtcctc aatggctgca aaatcttttg atgaacgaag
     1081 gcattcgtcc aatcaacaat gtagtagatg tgacgaacta tattttgctt ttattcggac
     1141 aaccactaca tgcatttgac tacgacaaat tgaaatcaaa agagatcttt gtccgccgtg
     1201 caaaagaaaa tgaaacatta gtaacactag atggagaatc tcgggaattg acaactgaca
     1261 atctcgtcat tactaacggc aaagaaccag ttgctttagc tggtgtcatg ggagggttag
     1321 actcagagat cacgaatgaa acaacaacag ttgcactaga atctgcttta tttgatccaa
     1381 tttcgattcg tcgtacagca aaacaattta atttgcgtag tgaatcctct gcacgttttg
     1441 aaaaaggaat caaccatgcg actgttggag aagccagtga ggttgctgca gcaatgatcg
     1501 tttctcttgc gggtggggaa gttctacaag gcgcggtcaa aggctcagag atcaaagcag
     1561 aagacgtatc agtgaagatt tcacttgaac gcatcaatca atatttagga acatctcttg
     1621 tcgttaaaga agtaaatgag atttttgaag cacttggatt tagctatgaa gaaaaggagt
     1681 atgtttattc cgtagtagta ccaccaagac gctgggatat ccaaatcgaa gcagatatta
     1741 tcgaagaagt tgctcgtatt tatggatacg accagttgcc atctacactt ccaagtggag
     1801 aaacggttgc tggaagcttg tcaaaagaac aagaaactac tcgtaaaatt cgtacaatct
     1861 ttgaaggaag cggattgagc gaagcaatca gttatgcatt gacaacagaa gaaaaatcaa
     1921 gacagtttgt tataaatgaa agtaatatta cacgtttgga ctggccaatg agcgaagaac
     1981 gttctgtatt acgaatgaat ttgatcagcg gactattaga tgatatcagt tataacactg
     2041 cccgaaaaaa tacagagatt gctttatatg aaattggtcg ggtgttcttc caagaaaatg
     2101 atccacgtac acatctgccg caagaagtaa aacatgcagc aatcgctatt tctggtattt
     2161 gggaagaaaa agattggcag acgaaagctc aaaatgtaga ctttttcacc atcaagggtc
     2221 ttttagaaaa cttatttgaa caactaggca tcactgaaga tgtccattat caaacagtga
     2281 cagaaatgaa ggaaatgcat cctggaagaa ctgctgcgat ttaccttggc gaaaaattta
     2341 ttggttttgt cggtcaagtg catccaacga ttacaaaaag ttatgatatt ccagaaacat
     2401 atgttgctga gtttgattta tctgctatag tggaagctgc tcaaaaaggg atcgttttcg
     2461 aagcagtgac aaaattccca gcagtcagcc gggacatcgc tttgcttgta aaagaaactg
     2521 tgacaaatca ggaattaaca gaagttatcc gttctgctgc cggaagattt ttaacagata
     2581 tccaattatt tgatgtctat caaggagaaa acattgaaaa aggacataaa tccatggcgt
     2641 atagcctgac attcgtcaac ccagaagcaa cattgacaga tgaagaaatc aataaagcaa
     2701 tggaaaaagt cgaaaaagcg ttggtagaaa acctagaggc aagtattcgc taagaaggaa
     2761 aatatagtaa gtaacgatca agtagctttt gaagaccata catttgaaaa aaagatgtga
     2821 cacaacttcg tcacatctct ttttttgatt ttaaaaaagg aatagcacag agacgagata
     2881 aaaaaacagg cctttacagc ctgctttttt agagtaaatg aattaatgga agtaattgat
     2941 tcccattgct gattttactt cagaaagtgt ttgtgcagct actttagcag cttcttcact
     3001 acctttacgt aagatttcca taacagctga aggatctttt tccaattctt ctcggcgcgc
     3061 acggatcggt gcaaattcag cttccaatac atcaatgagg tatcgtttga tctttacatc
     3121 accaagtccg ccgcggcggt aatgagcttt catttcttca attgcttctt tatctgttcc
     3181 gaatacatcc aagtaggtga agaccatatt cccttctact tgtccaggat cttgtacatg
     3241 gatgtggttt ggatcagtat acatactcat gacttttttc tgtaagacat cggcaggatc
     3301 ggagatataa ataccgtttc ctaacgattt actcattttg ccatttccat cgattccagg
     3361 taatctaccc attccttttg gtgggaatac gccttttggt tccaccaaga cttcaccata
     3421 tgtatgattg aagctttgta cgatttcctg tgtctgctct agcattggtt tttgatcctc
     3481 accaacaggt acaagattcg ctttgaatgc agtgatatct gctgcttgag aaacaggata
     3541 gataaagaat cctgtaggca cgctttcacc gaattttttt tgttcgattt ctgttttgac
     3601 agtcggattg cgccgaacgc gtcctacgct gactaagttc aaataataca tggttaattc
     3661 agctaattcc ggaatttgtg attggataaa taaagtagat tttgcaggat ctaatcccac
     3721 ggctaaatag tctaaagcaa cttgcaagac atttgatgaa accttctcag gatttttagc
     3781 attatctgtc agtgcttgca tgtcagcaat catgacaaac agttggttgt cttcatctgc
     3841 ctgcatagcc actcgggttt ttagagaacc gacgtaatgc cctaaatgca gcttcccagt
     3901 tggtcgatct cccgttaaaa taatattttt caaaaaagac acctctttac gatttgtaag
     3961 tctgagcact taatatctta ccgtttttcc tgaaaagaaa caacagttcc taaggaaaaa
     4021 acaaggaaag aggataaaat aactaataga ttagaaatgg aagaaatatt tatgggagaa
     4081 taaacgttct aagaaacttt acaaattact aaactatcag atataatgta ttttattata
     4141 catctttaaa agaaatctta ctaaataata atgggagtgt aattctatgg caatgattaa
     4201 atttgatcac gtggataaat attatggcaa gttccacgca ttaaaaaata tcaatctcga
     4261 attcgagaaa ggagaagttg tcgttgtcat tggtccttct ggttcaggaa agagcacgat
     4321 gcttcgctgt atcaatggat tagagacgat ctcttcaggt aagctgctga ttaatgatac
     4381 tgacttgcat gataaaaaga caaaactgac agaagtccga aaaaacatcg gcatggtttt
     4441 ccaacatttc aatttgtacc caaataaaac tgttctggaa aacatcaaat tagcaccaat
     4501 caaagtgttg aagcaagacc aggcaactgc agtcaaaaac gcggagaaat ttttgaaaac
     4561 tgtcaatatg ttagataaga aagattctta tccttccatg ttatccgggg gacaacagca
     4621 acgggtggca atcgccagag gtttagcgat gaatcctgag atgctgctgt ttgatgaacc
     4681 tacttctgct ttggatccgg aaatgattgg tgatgtatta gacgttatga aaaaattggc
     4741 tcatgacggt atgtcaatga ttgtcgtgac acatgaaatg ggctttgcca aagaagtggc
     4801 ggatcgtgtt atcttcatgg ctgatggaga agtattggaa gacagcagaa atgtgagaga
     4861 ctttttcgaa aatccaaatg aagtccgagc acagcaattt atcagtaaag ttatcaatca
     4921 ttgataggag gaaaaagatg cgcaggttaa aaaaaatacg cctgattttc cttttaggca
     4981 ttgcttttct tgcactagcc ggatgtaaag gaaatgggtt ggcaaatgaa gatattttag
     5041 agcgaagcaa atctaccaat gaaatcatct ggggtgtcaa gtatgatact cgattatttg
     5101 gaatgatgga tattgagagc agaactgtac aaggatttga tgtagatatt gcaaaagcaa
     5161 tcactaaaaa gatccttggt gacaatggaa aaacagaatt tgttgaagtc acttctaaaa
     5221 ctcggattcc attattgaaa aacggaaata tcgatgcaat tattgcaacg atgacgatca
     5281 cagatgaacg aaaaaaacag gttgactttt cagatgtcta tttcgatgct ggccaagctt
     5341 tgttggtaaa gaaaggaagc caaatcaaaa gtgtagatga tttgaatgct tctacaacgg
     5401 ttctagcagt caaaggttct acatcggctg cgaatatccg acagcatgca ccagatgcaa
     5461 aaattttaga attagaaaac tatgcagaag catttactgc tttacaatct ggtcagggag
     5521 acgcgatgac tacggataat gcgattttgt tagggattgc agatgaaaat ccagagtatg
     5581 agttagttgg tggaacgttt actaatgagc cttacgggat tgcgatcaac aaagggcaag
     5641 aaaacttctt gaaagctgtt aaccaagcgc tagaggaaat gcatgcagat ggcacatacg
     5701 ataaaatcta tcaaaaatgg tttccaaatg aaactgaagg aaaagtagag taagggggag
     5761 aggcaatgag cgaattgata caaacttatg gaccaatctt tgtcgacggg ttcaagatca
     5821 cattgttttc gagtgtctta gcacttgtgt ttagtttgat tatcggaacg ttaatggcaa
     5881 tcttccaatt atcaaagaat aaagtagtga gcgccttagc aaaggcatat gtagaatttt
     5941 ttagaaacat tccattatta atcatcgtga tgttttttta tgttgtcgta ccgatttatt
     6001 ggtttaatct agatggattc caagcaggta cgattggatt aacgatctat acatctgctt
     6061 ttattgctga aacagtacgt tcagggattc aaaccgtacc aaaaggtcaa acagaagcag
     6121 gactatcttc tggttttacc tattcagaaa tcatgcgtta tattgtttta ccgcaagcat
     6181 tcaaaatcgt tatcccgcca ttaggcaacc aatttatcaa cctcgtaaaa aactcatcta
     6241 ttttagcaat cgtagctggt ttagatttga tgtatcaagg agacttgatc gctagtgaaa
     6301 cattcaatac cttcgatacg tatatcatcg ttgggttatt ttacttggtt atcacgctac
     6361 cgttatctta cttaatggtt tatcttgaga aaaaatgggc ggttcgttca tagaaggagg
     6421 ggaaatacat ggattttagc ggcgcttttt catggatgaa tatccgtttt ttattggaag
     6481 gattaaaggt tactgttgaa gtttcattgt tttcgatcat ttttagtttc ttgatcggtg
     6541 gtctgacagg tgtgattcgt tttgcgaata tccctttctt ttccaaaatc cttggtgtag
     6601 tgattgatat tatacggaat cttccattat tattgatcat tttcttcact tactttgcct
     6661 tacctcaaat cggcattcag atgaacatct tctggtccgc tgtcgcagca ttaacgattt
     6721 ttgagtcagc tatgttgtcg gaaattttcc gagctggact caatgctgtt ccaaaaggac
     6781 agatggaagc aggattatca acgggactga cttatgtaga atcgatgcga acgatcgtgt
     6841 taccgcaagc atttaaatca atgatcccgg caattgtgag ccaattgatt tcactgatca
     6901 aagatacttc attagcagta atcatttctt tacctgaact ttctcatcag gcacgtatta
     6961 tttatggaca gaacaccaat tatgtgcttc cgatgttcat catcatgaca cttatgtact
     7021 tcgtggtatg ttacgcgctt tctctgcttt ccggttactt ggaaaagaga cggtacagtt
     7081 attaatagat gattctttta gcggttgtgg caaactaaag tcacaaccgc ttttcctctt
     7141 tccattcgaa aaaaagagat ttcgattgtt ttaagcaagt aagagcactt gaaaattcac
     7201 gctaaaaagt acacaatagg gaggatagaa tttattgaaa aaaaggatga tacgattgac
     7261 agataatcgc cctatcggat ttattgattc aggtgtcggc ggcttgactg tagtaaaaga
     7321 agccctgaaa caattaccga atgaaaatat tttatttgta ggagacacag cacgctgccc
     7381 atatggccct agacccgcgg aacaggtaat ccagtatact tgggaaatga cggattatct
     7441 ggtggagcaa ggaatcaaga tgctggtgat cgcctgcaat accgcaactg cggtggcttt
     7501 agaagaaatc aaagctgctc tttctattcc agtcatcggt gtgatccttc ccggtactag
     7561 agcggcagta aaaaaaacac aaaataaaca agttggcatt atcggtacga ttggtacggt
     7621 aaaaagtcaa gcttatgaaa aagcactgaa agagaaagta ccagaattga ctgtgacaag
     7681 tcttgcttgt cccaaatttg tttcagttgt cgaaagtaat gaataccatt catcggtggc
     7741 gaaaaaaatt gtggcagaaa cattagctcc tttaaccact aaaaaaatcg atacattgat
     7801 tttgggatgc acccattatc cattattacg ccccatcatt caaaatgtaa tgggagaaaa
     7861 tgttcaactg atcgattctg gagcagaaac agtaggtgaa gtatctatgc tgttagatta
     7921 tttcaatttg agcaattcac cgcaaaatgg tcggacatta tgccagtttt atacaactgg
     7981 ctctgccaaa cttttcgagg aaatagctga agactggctt ggaatcggac acttaaatgt
     8041 agaacatatc gaattgggag gaaaataagg atgcgtcatg acgggagaaa agcccaagag
     8101 cttcgcaaac tggagcttca aacaaatgtt ttcaaatatc cagaaggatc ggtagtgatc
     8161 cgttttggtg atacaacagt tatttgttct gcaacaatag aaaattctgt tccccctttt
     8221 ttgagagaaa caggaacagg atgggtgaca gcagaatata gtatgctgcc tcgagcaacg
     8281 agtacgagaa atcgtagaga aagcagcaaa gggaaacttt ctggtagaac aatggaaatc
     8341 cagcgtctta ttggtcgttc cttgcgtgca gtagtagact tggaaaaatt aggagaacgt
     8401 agtatcatcg tagactgcga tgtgatccaa gcggatggcg gtactagaac agcaagtatc
     8461 actggggcat ttgtggcatt gaaattagcc attgaaaaac ttttaagaga aaaagaattg
     8521 tcagaagatc caataaaaga acatcttgct gcagttagcg taggaatctt accagatggg
     8581 acttgcgtca cagatttgga ttaccaagaa gattccgcag cacttgtaga catgaatctt
     8641 gtaatgacag aatctggaaa attcgtggag attcaaggaa ctggagaaga agcaacgttt
     8701 gatggtgaac agttgaatga aatgctcttt tttggaaaaa acgcgattga agatttgatc
     8761 aaagagcaaa aacatgcttt gctaacagag tttgctcaaa atgatgagcg gatagaagaa
     8821 acaaaaacaa tcatcattgc gacaagaaat ccaggaaaag cagaagagtt tcgaaatatg
     8881 ttcaaagaag cgggatatca tgtaaaaaca ttacttgact atcctgaatt gccagatgtg
     8941 gaagaaactg gcagcacatt tgaagaaaat gcccgattaa aagcagaaac gattgcacaa
     9001 ttgcttgatc agccagtctt agcagatgat tcaggtttga aagtggatgc tttaggcggt
     9061 atgccaggaa tctattctgc acgttttgca ggggaacaaa aaagtgatgc ggggaataac
     9121 gccaaattat tatatgaatt gacagatgtg ccggatgaaa aaagaacagc acaattccat
     9181 tgtacgttag tattcgcggc acccaaaaaa gacagccttg tagtagaagc ggaatggccc
     9241 ggtcgagtag cccgtatacc atctggcgaa aatggctttg ggtatgatcc tttatttatc
     9301 ccagaaggta aggaacaaac agcagcagaa ttatcgagcg aagaaaaaaa caagatcagt
     9361 catcgggctc aagcaatgaa aaaattgagt gccgagtgga aacaatggct ggaaggagag
     9421 agatagatgc ggtatttagt agtaagtgat aatcatggag acagagatat cctcgttgac
     9481 ttagtagatc gttatcgttc tgaagtggat atctttttcc attgtggtga ttcggaatta
     9541 gaagcttctg acccattatg ggatacattc caagtggttc aaggtaattg tgattatgga
     9601 ccaggattcg aacagaaaaa agtgatccaa actggtcagg atacagtatt catgactcac
     9661 ggtcatttat cgaatgtccg atttggtttg acacagttag caatcgaagc tcgagctgca
     9721 actgccgata tggtgttttt tggtcatacc catcaaatag gctgtgagat ggatcagaaa
     9781 atcctgtttt tgaatcctgg tagcatttct cagcctagag gcccgatcca aatcccatca
     9841 tatgctctta tcgaaagttt agatgaccaa atttctgttc aatactataa ccgtgcccat
     9901 caggcagttg aagagttggc cttttctttt tcgagagagc gctgaataaa aaaactctaa
     9961 tttttaataa aaataagctt tattttatga attttgcatc cggtttcggt aaaatagtag
    10021 tgttgaatga aatagaaatg gaggccatca gatgattgga cctatagtaa gagaaatgct
    10081 gctggaaaat caggaaacgt ttttgattcc tgcagaaaat gtggcaaatg ttatgtatca
    10141 tcatccattg tcccatggtt tacttgtgtt atcgaaggtc ggttattcca aaattcctgt
    10201 attaggaaaa gatgatcact ttgtaggact agtaagttta tcagatgtgg tcaataaaat
    10261 gattgacttg caaacaatca gcatggaccc gttagaagga ctaacggttg ctgatgtgac
    10321 agaaacagat gttccgacaa ttgatgaaaa ttgggaacta gaagaagtac tccatttgtt
    10381 agtcgattcg ccttttttac cggttgtcac agaagatcgg attttcaaag gaatcattac
    10441 aagaaaagag ctgcttaaag ccgtaaactt catggtgcat gaattagaac gtcgtaatat
    10501 attgtctccg aaaatcgaag aattaaaaga acaaatcgat atcgtgagct aaagaacgag
    10561 aaactaaaag ataatcaaaa aataagtaga aaaaacagcg taccagtttt gactttatct
    10621 tgaaaaagag gctgggacaa aagcgtttaa ctccaagaaa taagaag
//
LOCUS       DBNICI010000100        10421 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.101, whole genome shotgun sequence.
ACCESSION   DBNICI010000100 DBNICI010000000
VERSION     DBNICI010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.101"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(189..1214)
                     /locus_tag="AC1ZCC_001988"
     CDS             complement(189..1214)
                     /locus_tag="AC1ZCC_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIQ1630229.1"
                     /translation="MKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVAVSLAI
                     FGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQRPSKVI
                     YDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAKKRGIT
                     ISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINSGELNK
                     DGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYNIQIVD
                     RVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYNLMNSG
                     GNGRVLR"
     gene            complement(1237..1755)
                     /locus_tag="AC1ZCC_001989"
     CDS             complement(1237..1755)
                     /locus_tag="AC1ZCC_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630230.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            complement(1701..2285)
                     /locus_tag="AC1ZCC_001990"
                     /pseudo
     CDS             complement(1701..2285)
                     /locus_tag="AC1ZCC_001990"
                     /inference="COORDINATES: protein motif:HMM:NF013884.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
     gene            complement(2414..3997)
                     /locus_tag="AC1ZCC_001991"
     CDS             complement(2414..3997)
                     /locus_tag="AC1ZCC_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HIQ1630231.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            complement(4016..4732)
                     /locus_tag="AC1ZCC_001992"
     CDS             complement(4016..4732)
                     /locus_tag="AC1ZCC_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylyltransferase domain-containing protein"
                     /protein_id="HIQ1630232.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            complement(4768..6189)
                     /locus_tag="AC1ZCC_001993"
     CDS             complement(4768..6189)
                     /locus_tag="AC1ZCC_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIQ1630233.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINIS
                     YIKQLCYRGRNIISMIKSREHKR"
     gene            complement(6186..7274)
                     /locus_tag="AC1ZCC_001994"
     CDS             complement(6186..7274)
                     /locus_tag="AC1ZCC_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HIQ1630234.1"
                     /translation="MKKILVLANTYYQIIVAIQMKNTIFSDEYVEIIISDHSKNTKEI
                     AKKIEEKKVFDGCHYLESKSFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFD
                     EFICFSMGEVWLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLK
                     KRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKY
                     IFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSS
                     IPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAY
                     DIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            complement(7358..8593)
                     /locus_tag="AC1ZCC_001995"
     CDS             complement(7358..8593)
                     /locus_tag="AC1ZCC_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630235.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGFFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYLTDNTLTYPDTQMI
                     FLLVPMIVSWVDNQQRISE"
     gene            complement(9211..10266)
                     /locus_tag="AC1ZCC_001996"
     CDS             complement(9211..10266)
                     /locus_tag="AC1ZCC_001996"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIQ1630236.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKLFIIL"
BASE COUNT         3518 a         1943 c         1114 g         3846 t
ORIGIN      
        1 ttttagttgt ctagcttaat tttacaatcg gcgctttata atatacgtat atatttgtat
       61 ttttcaatat gttaaattta tttttcaaaa cagtataaac gctcaacaat ttattttcac
      121 gttattctaa tcactactaa gacagataat caccataaaa attactgtga tttatcttta
      181 tatgttgact aacgcaacac gcgaccattt ccaccgctat tcattaaatt atatacatcc
      241 tcaattgtag ttcgattaaa atcaccttct atagtatgtt ttaaacaact agatgcagtc
      301 gcaaattcaa tagccttttg tccggacata ttttcacata acgcatatat tataccagca
      361 gaaaagctgt ctccaccacc aacgcgatca acaatttgta tgttgtattc tgtggaaaaa
      421 tacgctttat ctttttctgc ttcgtataac attgcactcc aaccatttct acttgccgaa
      481 taacttctac gcagagttgt agctacatac ctacatccaa acttcttgtg aatctcccta
      541 gccacctgca catagccatc tttatttaat tctccactat taatatttgt atgctcagct
      601 ttaattccta gcactttttc tgcatcttct tcattacaaa tacaaatatc aaccaatggt
      661 agtatctgtt ccatcttttc ttttgcttcc tctgatgtcc aaagcttgga tcgataattt
      721 aaatcacaag aaattgttat acctcttttt ttggcagcct gacatgcatc taaggacgtc
      781 tcaaagagat tctcagacaa tgctggattt atccctgtcc aatgaaacca atttgcatcg
      841 tttaggatct ccttccactt aaattcatca ctatcagcta acgaaattgc agaaaatttt
      901 ctgtcataaa ttactttaga tggtctctga gctgctcctt tttctaaata atacagtccc
      961 atgcgtccct ctccaaatat aatattatca acatttactc caaaataacg caaagcatta
     1021 gctgccgcat atccaacatc tgaatccgga agctttgtta taaattctga ttgatatcca
     1081 aatattgcta aggataccgc tacattagct tcccctccgc agaatgtagt ctccaaacta
     1141 tctctttgaa ataactttga ataaccgggt gctgcaagtc tcaataataa ttctccgaat
     1201 gatataactt tcacttcttt tctcctcatt tattaattat gggtatatat ttataacatg
     1261 attcataagt ttacacaggt tctttacact cttaatttta aaaatctgtc tttcaaattt
     1321 ttctcttaca acatcgatag ccatgcaaat tatgcacaca actacaactg caattataaa
     1381 ccaaatccaa aaatccctat ctaaaaacct tttgcctaat ccaaatatcc catcccatat
     1441 taagcctcta gacttcatac catcaatgaa ataatgattt tcatgtaaaa tatatacccc
     1501 taaactggtt ctcgcaagtt tatttatata cttactgtaa aatgaaaaat ttttaaatac
     1561 ataaaataaa aatatacttg ctataaccat caatggagaa ccaatatgaa ccatctggtt
     1621 aaccattgca tttggtataa atttgaaaag tattcctatg gatatgagaa gtggtataac
     1681 tgttatacat acaattgttc ctatgattct gtgtctctca aaaatattat tatcgttttt
     1741 ttcagtgtat aacatatgac atatagtgca attaaaacag cacaatcccc aaacaaagat
     1801 gttttaaata acatacgata acagcataca cctataaaca ataacatact tattttttgc
     1861 aactgaagtt tttccagatt gttaagcatg atatttaaat acggacttag aatatacaat
     1921 gctaaatagg atgtaataaa ccaatatcta cctagagcaa atgataatat ttcttttaca
     1981 aagctgttca atgaaaatgt ttctattcca catgcacaaa gaattccata aactaataca
     2041 ctataaaaaa tacattctaa tacaattccc aaaacatttt caaagtgaat gtttcttttc
     2101 tcacttaaaa aatatgccga aataaatata aaacaattaa ctcctagctg accccaaata
     2161 ccaatataat tcaagaaaat ctggttagca gttaatgaac catgtactat aattccgtct
     2221 gcattataat ttaaacgcaa attagcatgg taagcaacaa tacacaatat agaaatgatt
     2281 cttaaaagtt caaaattaga gtttcatctc gccccatcgg cccttaaata tttttcttct
     2341 tttttattta agactttacc catttttatt ctctcactat ctctttatca aaattttaaa
     2401 aacattatta ctactactta tcaattcgaa cccaacgtgg atccgttaaa aatgtcacct
     2461 ttactttttt agctaattct gacaattcat ctctcatttg cacgttcatt gcatctaata
     2521 atgcaaattt tgttataatt tccatggatt gctcaccata attctcatct tcattatgtg
     2581 aatatccatc aaatccagct aagaatattt catccacatt catatcggct aaaaatctaa
     2641 ttgccattaa tcctgcattg tctcttacaa cttccctttc agataacaac tttttataac
     2701 tcgtccgtaa atatacatcc gatgcttgta tatttgatgt aacaatacat ttagttctgc
     2761 tttctttcgg taattcatga aaacgtctta aattacttaa aaatacgtaa tctatatcta
     2821 tatgagaata ttcaaaattt acactaatta ttacgacttc ttctttggta acaaactctt
     2881 ttattctttc tacctcatct acagaacttt ttcctggtgc aatcagtaga atttttttgt
     2941 ctgagagaaa ttttctcagt tcttccatat attcctcctg tattttccca gtttccatat
     3001 atttgtagta taaatcttct atatattgtt tatcataaga tacctttttt tcttcatcca
     3061 tcatttcgaa tatttcattc attgcttcaa ttgttaaagt attcttatat gacagatagc
     3121 ttgcataatt aggatgagca ttatgaactg ctgaaatata gtttggtaat gaatatcccc
     3181 aataactctt ctgatagaaa ctatttagta tttcatcaat tatatataat aatggtttta
     3241 attgatattt ttttcctgca ttatcattca aatattctac aaatagttct gtgttaagat
     3301 tacctgctcc tcttcccatt ccgtacaccg aagaatcaat aatcaggctt cgatttgttt
     3361 gtacggttat aagtttttgt gcattagaat atgcaagctg catgttatta tgtgagtgaa
     3421 aacctatctg tatatctttt tctagattat gctcaatcat gtaaaaaaga cgagttaaat
     3481 cttttccttt catcattcca aaactatcaa caatataaaa agcgtatggt ttaaactgat
     3541 tcacattttt tattactgct aaaaactctt catctgtata attgagagat accatagctt
     3601 gaacaaaaac taagtagcct tttttcttta tctcacgaca taatttcaac gcttcattgc
     3661 aatttttttt atgaaacgca atcctaattc catcaataga tgaaccatca taatctggaa
     3721 gatcctctat attatattcg ccatagttca tcattgctac aaataatttt ccttctcttt
     3781 tttcaggaat tatctgtgct aattcttcta aggtcgtata ttttgtaaca tttttattgt
     3841 atactgtttt atttgtgaga aacccacatt ctataatctc aattcctgat tctataagtc
     3901 cagaaataat tttttttgta ttatcaaatc caaattccca attgttacaa tagcccccat
     3961 ctctaagtgt gcaatctaaa atctgtacat tgttcatcct aaccttcccc ctgtcttaac
     4021 taccttgtaa aataagatga ttataaatcg catcagcaat cataaaatcc tcggcttcat
     4081 caatatcaac tgactcaatc tcacctactt caactatgca aggttttttc ccaattcttc
     4141 tatgcaactc ggtcatcaca ttgcgattat aaatataaaa tccactagtt tcttcgtata
     4201 aaggcggcaa atcctgtgtt ctaggaatat tgtccaattc ataattaaat ggttcaccgt
     4261 ctttccataa gaaatcttgc aatttttttg cagcaaatgc agaatcatat tctcctgtca
     4321 gcacggcatc tagtcctttt tcaatagatt tttcgctaat aaatggtgct gttgtgtgtg
     4381 tcattacata tacatcagcc tctacttctt ttgtgaaacc ggctaatacc tgtgtaatac
     4441 tcgttgtatc ctgatctaag gaactagatc tctttaaaaa tgttacacct tctggtataa
     4501 attctttaat atctgggtta ctacaataga catacacttt atcaatctgt tttactttta
     4561 aaagtgttga aagtatataa taacacaatg gttttccatt ggtaaaagct ttcgtgtttt
     4621 tttgtggcaa tcttttgtta tttaacttca ttggtactat agcgactatt ttcatacgac
     4681 atcctatact ctttttttta agaatatttt tcctttcata attatcgacc attaataaat
     4741 tatatctttt tataaaaata acatcgtcta ccgtttgtgc tctcgactct taatcattga
     4801 aataatgttt cttcctctat aacacaactg cttaatataa ctgatattta taataattaa
     4861 aataatcata attataattt ctattacata atttttaaaa tatatttcta tgattgcctg
     4921 aacaaacaat aatccccaag attccacaac aatatgataa tgctttataa aaatatcttt
     4981 tcccacagct cgtttacgaa caacatatat tacatatgaa catataacag tggctattgt
     5041 ggcaccttga acccctatct tataaacaag gactacactc ataatcacat taactaatgc
     5101 ccctatgatt gcagaccaca tcatggattt agaatctttt tttgctgata agataggtcc
     5161 taacagacct gatgcacaat ttaaaacatt agctattaat aaaaatggaa catatttcca
     5221 cgcttcataa aagtctttcg cataaagaat atgtgaaaac ggtctagtta atatgataag
     5281 acttgaacag gttatgcaca ttaacaaatt aatcataaca aatgtatttc cataaaattt
     5341 tgcagtctca ttatctccat attctttaat tgcagaaatt tgccaggcct gtataaatat
     5401 tccctgcaaa gtattaatta tttgaggtat tttatatgcc acagacaata agccattcgc
     5461 cgagacacct actataaagg aaaccacata cctatcagct gtactattaa tccaccatcc
     5521 aacaacagtt gcaattaatg gaatcgagta atttagcatt tctctcttta gctgaggatc
     5581 tttagcctca aaatcgatat attcccatat tctcactaat actgacagat aagtagctga
     5641 aataatctgt gataaaatat ttgcaatgaa aaaaccaatt aatccccatt tcagaataag
     5701 caagaagaaa atattggcaa ttattgttac aattgtgctg ataatacctg ctatgcccat
     5761 agctttaact ttttctaacc ctttagcaaa ttgtattaga aattgattcc atacatagga
     5821 tatataatac aaaaaaatat atttttctaa tccataaaca tcttgccata attttgtaat
     5881 atttaacaaa tacattacgg atccaaagac tatcacgctt aaaaatataa ctttaatcgc
     5941 tatagatgct atattttttt tatttttatc tttatccatt aaaaagcgca tcacagcatc
     6001 tacaatgttt aaggttaaaa ttggaaacaa taatgtggct gttgaaattg tcaaatcata
     6061 tgtgccatat tctgttgtgg ataatacact tgtatataaa gggactaaca aaaaaactaa
     6121 aatttttgaa gaaaaattac ttattgttaa tattcccata tttttaatta aatatttagt
     6181 tttgctcata gtatatcatc taagctttct gctattttca cattctttaa aatgtttttc
     6241 atcgtttttt gcttaagcaa tcgctcaata tcatacgcat tctttttgca agacggattt
     6301 ccttctatgt tacacaattt atacatataa tacgtgcgta caggttttgc agacattgta
     6361 ctacctgaaa taacacttcc tgaattaatt gtcataaata ttttatcgtt aaaatcgcct
     6421 gacaattgaa tcgcctccca aggtattgat gaatttttat cctcgataaa tccattatct
     6481 ttataaattg ttcttacatc tctaggatgc gtcttaacaa ttagattatc ttttcctact
     6541 aagtctgcta ttttgcatac taattcatat tctccgacag gttctccgcc ctcaaaatca
     6601 taaacagacg taaaaaatat atattttttt tctttataaa tatctatcat agggttaaaa
     6661 atatccgaaa gttgcaatgc ggttcttgac ccagctgtaa ctaatggaat ttgtattgta
     6721 ttctctcttc ctgtatatat attaggaaaa aaacaataaa aattatagct tttgttatat
     6781 agtggagtcc ttttttttaa gtaattgagt actgcgatag cttttgttct tgctgttgga
     6841 ataatgtcaa ctccatagct taaaatacct tcttctaaaa ttgatacctg tacattttta
     6901 ttatttcgat ataagcaagc atatattcct tccaaccaaa cctctcccat tgaaaaacaa
     6961 ataaattcat caaaatattc acttttacag tttttcaaat aataggcata tcgatttgat
     7021 ttaccacaag aaatctgaag acagtcctta atcttttgaa aatacccaat attatcatca
     7081 aaagatttgc tttctaaata atgacagcca tcaaacactt ttttttcttc tatttttttc
     7141 gcaatttctt tagtgttttt agaatgatca gaaataataa tttcaacata ctcatcggaa
     7201 aaaattgtat ttttcatttg tattgctact attatttgat aatatgtatt tgctaacact
     7261 aaaatttttt tcaaattttt tcaccctact tcctgtccgt taaatattaa cttttacaac
     7321 taattataaa ttcataatta aaatatactt tagctatcta ttccgatatt cgttgttgat
     7381 tatcaaccca cgaaactatc ataggtacta agagaaaaat catttgagtg tctggataag
     7441 tgagcgtatt atccgtcaaa tataatataa acatatatat agttaataat aatacacact
     7501 cccctgtttt caatccaaat tttttagtta gtcttatcgg tttcacaaaa aataaccaaa
     7561 acatccatat aataaaaact atacatccaa cttctatata aatagttaat atatcactgt
     7621 gtaggcttgc tgcaattctt ccaccattta acctaaagtg cgataaataa agattaaaaa
     7681 agtattttga aaaccatgtc catcctgttc ctacatatcc taaaccaagt tcataaaatt
     7741 tagatgaata tagataaaat gaaagccttg caccgtcatc tgtaatgccc aaatttgaca
     7801 taagcaatgc caaaattcct gtatctataa tgtaaataaa cactaacgaa ataacagttg
     7861 cacaaatagt acacactaat gcacggattt gaatcttttt tccccacttt aataaaacat
     7921 aatacactag aattgcacca cttaaagcta acagttcaat tctcttcaat cccatgaaaa
     7981 ttaaaattaa agatacgata atttttatct tatgtttttt tgtattttta ttttcaaaaa
     8041 atacgtaata aaggaaaaaa aaccccatcg caaaagttaa atcatgtacc tccatagcaa
     8101 aatttgcagt atttgtttca cctgttataa gactttttat ataagaaatt aaaccattta
     8161 aaccataaga tacaattaaa taaaaaactg aaccactcat atatgaaata acacctgacc
     8221 aaaaaataaa atcaattcct tctgccttaa ataagtaaaa tgctgtacat gcgtaaccat
     8281 atgcaagaat taaataaagc aacgtggaac ataactttat aaaattttca aattctgcat
     8341 ttgaaccaat tccccatatt attagagtat agatagaaaa tatcacaaac ggcattatag
     8401 tcaaccaaaa gttattcttg ataacgtatt ttttatatgt tttctcacga taagaaaaat
     8461 agcttaacat aaaatataaa tcccatattg ccataataaa atatcgcata gataaactag
     8521 tattaattcc ccaaaatcgg gatgttaata caattattgt acttaccata aatagcattt
     8581 tttttacact catcgtttcg ccccgtctac atatatttgt tctatatacg agtttaataa
     8641 actgatattt tttgcgcttt aagtgaagaa cattaaataa tggtattgta ctgatttttc
     8701 catacaagtt ttctttaatg taacaataca atatatttaa tttctatttc cagtcatcaa
     8761 ataaaagtaa atttcagctt ctcattgtat atagaaattt caaagaaact attctctcaa
     8821 tttctttaag ttaatccctg caagattatt ttagaaacta cttttttcat ataaccagaa
     8881 taatttaact attcactcta acagttaaat tattttctcc catcacatga ctagaagcac
     8941 atcttctcaa caaacttttt caaaatgatc actcatataa ttacaccttc attaatttaa
     9001 acagcactac aaattccaat aattaatgag catatcgtat ttgtatacaa aaaaatcgtt
     9061 gttaaggcaa aaatagccgt ttttacgctc attttatata ctgttggaag gtgataatcc
     9121 acattactat agtttacttt ttagaaaata ttattcttta taatattttt ttgtaacttg
     9181 ttaatttcta ttatttgata tacttttagt ttataatatt ataaacaatt tttttctggt
     9241 catattttta attatctttc taattctact taacaacaac aatattgtat aacacttaca
     9301 ttcaagcatt ttatgaaaaa atttttgttg aaatggtagc gtatttgtag gatatccctt
     9361 ccaacattga attaatccct catcttcaat aaattttttt atatactttc ttttctcttt
     9421 aattcccaaa tttgcatcat caactaagcc cacataaagc ctacaataat caattgccat
     9481 acgttttgct ctgatttgac attctaaatc ttctttcgat gctatagctt ttagagttat
     9541 taaaaattct ttaaattccg tctgcttatt tggagataaa ctttttgtaa tagactcttg
     9601 ttcttctttt ctataacaat aatctgtata ttctgtaaaa gcaattttat ttgcgacatc
     9661 atatgcattc aagttaaaaa ttgtatcttc agataaaatt ttttcaaatc ttagtctata
     9721 tttatctagc agatttttat tatacaaaga catacatact gacatcggga aggcctctac
     9781 ttcttgatct tttggactat gtccaaataa attttttcgt acatttttaa tactattagg
     9841 cgtatcatat acattcccac tcaaaggatg ttgctttttc accatagctt tgtcattcgc
     9901 aaaatcacaa tgtccactaa ctactatatc agcattttct ttggacgcaa ctaagtataa
     9961 tttttcatac atatcacttt ctacccaatc atctgaatca agaaaagcca catattctcc
    10021 tgtcgcattc tcaattcctg tatttcttgc cggtcctaac cccccatttt ctctatgtat
    10081 tgtttttaca ttagaatatt ttttttgata ctcatctgct atttcccccg aagcatctgg
    10141 cgaaccatca tcaactaata cgatttctat ctcttttaat gtctgactca ataaactatc
    10201 cacacatttt cttaaatatt tttcaacatt gtaaattggt actatcacac ttaccttgtg
    10261 tttcataact ttttatacct ctaatattta taataatttg ttttctacca acatcccata
    10321 aaaagtaact ttatcatctt tttgcttttt aaaagattgt ttccatgtca acttaaacag
    10381 taaagtatct ttctttaata agttccatac tttttcatca t
//
LOCUS       DBNICI010000101        10290 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.102, whole genome shotgun sequence.
ACCESSION   DBNICI010000101 DBNICI010000000
VERSION     DBNICI010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.102"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(18..770)
                     /locus_tag="AC1ZCC_001997"
     CDS             complement(18..770)
                     /locus_tag="AC1ZCC_001997"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIQ1630237.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            883..1803
                     /locus_tag="AC1ZCC_001998"
     CDS             883..1803
                     /locus_tag="AC1ZCC_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIQ1630238.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            1876..2811
                     /gene="murB"
                     /locus_tag="AC1ZCC_001999"
     CDS             1876..2811
                     /gene="murB"
                     /locus_tag="AC1ZCC_001999"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIQ1630239.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3109..4587
                     /locus_tag="AC1ZCC_002000"
     CDS             3109..4587
                     /locus_tag="AC1ZCC_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1630240.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            4864..6924
                     /locus_tag="AC1ZCC_002001"
     CDS             4864..6924
                     /locus_tag="AC1ZCC_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HIQ1630241.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7007..7576
                     /locus_tag="AC1ZCC_002002"
     CDS             7007..7576
                     /locus_tag="AC1ZCC_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1630242.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7751..8557)
                     /locus_tag="AC1ZCC_002003"
     CDS             complement(7751..8557)
                     /locus_tag="AC1ZCC_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630243.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8695..10209
                     /locus_tag="AC1ZCC_002004"
     CDS             8695..10209
                     /locus_tag="AC1ZCC_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HIQ1630244.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3651 a         1714 c         2032 g         2893 t
ORIGIN      
        1 actttttcac aacctcttta tttcaaatct aattctactg gacaatgatc actgcccata
       61 atgtccgtat ggatctttgc atcagctagt cctggctcta aatctttgct cgtcaaaaag
      121 taatcgatcc gccaaccagc attgtttttc cttgaattga atcggtagtt ccaccaagag
      181 tagacacctt cctgggttgg ataaaaataa cggaatgtat cgataaaccc gttatccaac
      241 agtcgagaaa gagccgctct ttcttctgga gtgaatccag catttttttg attcgttttc
      301 cagtttttca aatcaatctt ttgatgagcg acatttagat ccccacacac gatgactggt
      361 ttttgttttt ttagattttc caaataatta taaaaggctt cttcccattc taatcggtaa
      421 tctaaacgtt ttaattcgct ttgagaattt ggcgtgtaac aggttacaag aaagaaatcg
      481 gaatattcca aagtaatcag acggccttct gtatcatgga tatcgatacc cattccgtaa
      541 gtggcattca atgctggttt tttagcaaaa atcgctgtgc cggaatagcc ttttcgttcc
      601 gcgtaattcc aatattgata ataacctgga agatctaaat caatttgtcc tgcttggagt
      661 ttcgtttcct gcagacagaa aaaatcggcg tctaactcat gaaaaacttc taaaaaattt
      721 ttatttacga tggcacgaag cccgtttacg ttccaagaga taaatttcaa taaaagcaca
      781 ctccttcgaa ttaatttcct tacctttgcg atactattgt acctcatttt agtaaaaaat
      841 ggtgtaagat gaaagtaact aaatgatcca ggaggaaagt agatgaagat tggtgtaatc
      901 ggtgtaggga atatcgcaga aaaagcatat ttaccaactt atgcgaaaaa acaaggaaca
      961 cttgattttt attttgcaac aagaaataaa gaaacaaaag aaagaattca aaaagagtac
     1021 gggttccaac atctatatga aacgatcgat gagcttttag aagaaaaaat cgaagcatgt
     1081 atgatccacg cagcaacaaa agtacattat gaattagcca aaaaatgtct agaaaatggt
     1141 atccatgttt atattgacaa accactgagt gtccatctaa atgaagttcg tgaattgcaa
     1201 gaattagctg ataagaacca aataattttg atgatcggtt tcaaccggcg ctttgctcct
     1261 atggtagaag aattgaaacg tatcccagat aagcggttga tccacctgca aaaaaatcga
     1321 atagcggcaa aagaaacgac agagttcgtc ctttatgatt tgtttttaca tcttattgat
     1381 acagctgtgt atttattaga tgaacccatt ttgagaacaa aataccagat aagagaaaca
     1441 aaggaatttt tggaatatgc cattttacaa ttagaaacac aaaatcaaac ggctatttta
     1501 acgatggatt tagcaagtgg cgctaatata gaaaaatatc aagtaacaag taaaaatggt
     1561 acttatgaag taaacgatct gacccagctt gtcattcaag atgctgatgg aaaaaaagtg
     1621 aaagaatttg gcgactggac aaacacgcta gtcaaacgcg ggtttgagcc aatggtagag
     1681 gccttctttt ctcagatccg tttaggtaaa tcggaaacag aattgcttaa acaaaaagaa
     1741 gttgtaagaa gtcatgaatt atgtgaggaa gtgcttaaag atcactaccg tcatcaactt
     1801 tgaacaagac tggcaagatt gtattaagcg tgatagaata atgaggacgt ttgagaaaag
     1861 aaaggaaaat cctcagtgaa taaagaagaa atagttgaaa gcttagccac gattactata
     1921 ttgtttgatg aaccattgat gaactatacg tttacgaaaa caggtggacc agctgatgtc
     1981 cttgcattcc cgaaaaaaca agaagaagta aagcaaatca tcgattattg ccgaatccat
     2041 gatatacctt ggatggtatt aggaaatgca agcaatctga tcgttcaaga tggcgggatt
     2101 cgcggtgtag tcatcatgct gactgaaatg aaacagattc atgtcaaagg aacgatggtc
     2161 attgcggaag caggtgcctc actgattgat acgacttatg cagctttggc agaatcattg
     2221 actggatttg aatttgcttg cggcatcccg ggaagtgtcg gtggagcggt ttatatgaat
     2281 gcaggtgcgt atggcggaga gatcaaagat gtttttgctg aagtggatct tttgcttgaa
     2341 gatggaacat tgaaaacttt gacaaaagac gaaatggtat tttcttatcg tcacagtaaa
     2401 gtccaagaat tacgtgctat cgtattggaa gctcgattca gtttgcaaac aggggattat
     2461 gaagcaatca aagcaagaat ggatgagttg accgaattgc gtcaatcaaa acagccatta
     2521 gaataccctt catgcggaag tgtctttaag cgcccagtag gacactatac aggtcagctg
     2581 atccaacaag caggattgca aggcctgaaa tggggcggtg cacaagtctc tgaaaaacat
     2641 gctggattca tcgtcaatat cgatcatgcg acagcgacag attatattga attgattgcg
     2701 cacatccaag aagtaatcaa agaaaaattt gatgtgtctt tagaaacaga agtgcggatc
     2761 attggagaaa aacaagcaat atcagaaaca agcagaagtt tagccaatta ataagtaatg
     2821 gaacttaaaa taagtaaaag agcaggacgg tggccgtcct gctctttttt gtggttgttt
     2881 acgcaaatgg cgcaaatctt gtacatttta acacaataaa acaaaaaata tagaatattt
     2941 cctcataaat ttttccattt tttgcaatat tctcaaaaaa aagataaaaa atgagaataa
     3001 ctgatgaaaa aacaagcata atccatcata aaatcagtat aggtgaaaag atgaactttc
     3061 atctgcatat caagtaattg gtatgaggca ttttttgagg tgtaggatat gaagttggat
     3121 taccttatag aagaggaaga tagagaaaaa ctgctgctag ctagatattt agaatttgag
     3181 aaaaagaatt atgtagtgct gcctaagtta tatgaaacat taggtttgtc taaatttaaa
     3241 acaagaaatt atatcataga attaaataaa gaatttactg aattcaaaac aaagccagag
     3301 atccaaattt cagaagaagc agagatccat gtcgaacgtc tgaatttgac ggatatcaaa
     3361 aatttaagaa gaatctactt tcaacgatca aaaattgctc gactgttaac ggagatcgtg
     3421 gattcaaaag taacgatcga aaaatttgca gagaatcatt ttttaagcat atcaagagcc
     3481 tatgtaaaaa gaagagaatt agtctctttt cttgaacagc atcaaataaa gttaaagaaa
     3541 aacatattag ttggggaaga aataaacata cggaacgtat tatttggggt atatttcggg
     3601 gtctttaatg gtttttctat gccatttaga gaaagcgtca tgaatgaagc aaagagtatc
     3661 gtgcaatatt ttgaatatct gttcaattta aaactgtctg caacaaaacg aatcaaattc
     3721 aatttgctaa ccactattag tttgttgagg gcctatggga atcaaccaat cggaaaacgt
     3781 ttcttttcag atgaatactt taagagctca gaaggaatga agatcatcaa ggatttatca
     3841 catatttctt ttcttcatat ccctaaagag cagatcattg atgagatttc ttatatcatg
     3901 ttgtttttga tcacggaaga agaatttgaa ttagatggcg catttggact tgcggaagct
     3961 cattttcatg atatccacat gattagtcaa aaaatgtcag tggaattgat tgatcaatta
     4021 ccattttcga ttgaaatctc tgaagaagaa agactatata tacgaaaaga acttcgtatg
     4081 ggtatgatgc atgtcaatcg aaaaaattgg atattcgact ttgagatgac tagctttacg
     4141 tctaaaaagc agctacagtt tttcttcgag acgtatccaa tattcagtac agcaatccaa
     4201 aaggtcatac aagagaataa acgctatttc ttgggaaaag atgaacgttt catgattcgc
     4261 ttattttatg attatctctt tttattggtc ggtatttgtc cggtatcata ttttgaagca
     4321 ccgattcatg tctgtattga tttttcgcaa ggatctgctt ataccgatta cattatttct
     4381 caagtgaaag gatttaaaaa ttacaatatt gtgattgaga gtcgaatcac ttctcgtacg
     4441 aatatttatt tatctgattg tttacttgaa aaatttaata aagaacagat aatttggaaa
     4501 aatccaccga ctccagatga ctgggaatat tttggaaata ccatcatcag agaaaaaaat
     4561 agacatttaa ctgaaaaaat cagctagtat ttatggatgg cagctttgtc tcacatattt
     4621 attagctgat ttatgttttt ttcaaagaaa aggtaactag atataactag atataactag
     4681 atatattact taacaaaatt aaaaagtaca taagttatta ttccgatttt cttgaaacaa
     4741 taaaaaatca aaaaaaatac tctcctaata gtccatcttc ttttgatttt atttatataa
     4801 tgaaatcatc ttatgaaaat tactacttca tgtttagaca agagtaggga ggactaagaa
     4861 taaatgtgtt caaaaagacg ttcaaaacaa ctaaataaac acgcttgtcg tttagataag
     4921 gaacggtcgt gttctgaaaa acactacgtt aagtttatga tgcgcacgat gctgatcagt
     4981 ttatctgtag gtgtctatat gcatgtaccc gttcaggcaa cgacacaaac catcaaggtc
     5041 gtacgtacct ctgtttctga gaccactgat tcccctatca ctattactca tactttcact
     5101 gatgcaaatg gaaaacagct acttcctgca ttgacttcct ataataaagt cagttatcct
     5161 ccaacgataa accactactg gttaattcct aacaaaacat tatggacatc ggaaaccgga
     5221 agcagtgtgc cgctttctat cttttccgat atgactgatc ctacaaaagg tgatcagtta
     5281 aaacagatca ctgatatttt gaatcatgct ttgacagata taaaggacgg tggaagcgca
     5341 caagtttctt atgtatatga aaaagattat tcacagtttg aaagaaagaa aatcgatgtt
     5401 ctggcaggga caaaagtttc gatatctgat ctggtagaaa ggctgaagaa cgcagatgga
     5461 acacaaggag atatgtccaa agtaatttca aacaatggga atatcgtgga tacgactaat
     5521 acaggaacat ctactatttc cttgtcctat tttgatccaa ttgctttaaa aacaatgact
     5581 gcatctgctg tgatccatgt tcgtgctgat caaacagcta tcaaaggaaa agatcttcac
     5641 ttaagaacag gagagagtta cgcccctaaa gatcttgttg aatatgtaac aaaaagcgat
     5701 ggaacttcag gaaataaaga agaaatcaaa atgaacggga cagtagatac tacaactccg
     5761 ggagaatatc ccgttgtatt aacttatata gactcgaaaa catcaaatgt ggcagaaaca
     5821 acagctgtag tttatgtaga agcatctgct gcttcatcga ctgtcatcac tcattttata
     5881 gatagtcaaa caaataaaga acttcaagca gaaacacata tgagtagtgt aaatcgtatt
     5941 cctgatttgc gcctacctgc tgaattttct gatcgagtgc caaatataga tttatcgact
     6001 tatgaacaaa atgggaaagt cacttctatt tctgattgga tgcaagcaaa cggattagac
     6061 agaaaaagtc actggtcgga gatccttgaa gtacttcact cagaactgat gaataatagg
     6121 aagcaatcag gaggggaaat tacctttact tatgtttata taccagacaa tcgttcatta
     6181 gaaggtcatg atcttattgt tcatccaaac gaaaaaataa acaaagaaga gttgatccaa
     6241 tcagcaacaa attccttagg taaacccgta ccgttaaagg atgtatcggt tacaatcaat
     6301 gaagtaccat tggaggatgg ttatttgacc aaacaaacag gtgattttac gattatgtat
     6361 agttatcatg acccgtattc actccaagaa ctccatgctc agtcactatt acatgtttta
     6421 ccattaaaca aacagataga tcaaaaagaa caaaaaagac aagaagaaag cttgtcagaa
     6481 aaaaaacaac aaatggctca tgcagtttct ccaacaggga aagtaaaaca aacaagaagc
     6541 ctagagttag caagaatatc tagaaaaatc tcagaatcta ataaaacgat aaggcaagaa
     6601 gctcatttgg ccccacaagt ttctggattg gaaaatgaaa ttttagagcc taaaataaaa
     6661 caacaatcag aaaaagaatt tgttgctaaa cagtcggaag atcagaagga atcagatgac
     6721 cagaaggaaa cgaagaaaaa acgcttgtca actattgatt ccaccgatga gcatctgcag
     6781 ccgcaagtac cacttcctag tccgggaaca cccgcacctg gtggaggtat tccttcttct
     6841 gctttaggag atttcatgcg aggtatggca ggaacatggg tatacgctaa tcgaaatgaa
     6901 tttgacgcag atgactggtt ctgatttata tctctatctg ttatagtcaa ataagtagct
     6961 gagtcatatg ttatttggca taatggagac gaggagggat agaaaaatgt tagataaaac
     7021 gttaccttac gcagaaatct ggatgacaag accacttcaa caaaatgttc caatcacgcc
     7081 tttagcggaa ggttttgaat ttcgatttta tcaaaaagaa gatgaatctg cttgggcgga
     7141 gattgagact tccgttgggg aatttcaaac gacagatgaa gcacttgagt attttcaaca
     7201 aaactttgct ccttatcctg aagaacttga aagaaggatg ttttttgtag agacaaaaaa
     7261 aggagaaaaa gtagcaacct gtaccgcttg gcgaaaaaaa tcaagagagc aagtgtatcc
     7321 ggtttttcat tggttagcag tgaaaccaga ttaccaagga aaagggctcg caaaagcatt
     7381 gactgccaga gtcttgcaag aatttcctac cctccatacg gacggaccaa tctatcttca
     7441 cacacagaca tggagccatc aggcgattgc tctttataaa aaaatgggtt ttacttttat
     7501 tgctgaaaat ctagacggca cacctaatcc agattatgaa aaagttatgc aggttttaag
     7561 taaattagat aaataacgaa atggtgaaat aagtcctcag tattgggata aaaaacgaaa
     7621 tagtcattcc tcatatctta cttttttgag ttaatactat cggataacta atggcatgcc
     7681 atacattcaa aaaagaaggg gcagtgacaa aagtattgtc actgcccctt ctcttgtttt
     7741 ttagcatgtt ttatcgtttg atttttttag gaataaaatt cttgatgaac gtaaattttt
     7801 ggtaatcgtt tcgactatag aggtaaccaa atacactgaa aacaactgct ccaataaaat
     7861 taacaaataa atccttcata gtatccatga ttcccacgtc taagtaacca ttttggatga
     7921 ttgttttatc tgttttacca tctgcatttt gactttcaat gattgtccga gtgatatctg
     7981 gaacatgata gacagtgttt ccgtctttac ttaactccac actattgatt tgctggatga
     8041 tcgtatcttt ttgcatatcg aattgcagta atttatctgc tgaaaattcc acaaactccc
     8101 acataacacc aactgtcatc gaaaaacaga aggaaacaac tgctagtaat aaaggagtca
     8161 agttcatggt ttttgtattt ttattgagca aataaacaag ggaaaagccg acacccccag
     8221 ctaaaaatcc gctgaatgca tgtagaaaag tatcccacat tgaaaaatag ccataaaaat
     8281 cacttaactc tcctaaaact gttgaactaa aaataaaaga taacaaaatg atttctagca
     8341 tatttggaat ctgtatcttt aatatctttt ccagaaaata agggatttca aataaaatta
     8401 atgtcagtaa taaaagcatt gtatttcccc agtgttggtt ccagacctga tccactagca
     8461 taataacgac gatgatacgt aataaaatat agattccgtt tgtgattcta cctgcagttt
     8521 ttgtactgtt cgtaaaaaag gttttcaaat gattcataaa ataactcctt catgtatatc
     8581 cttaattata ctaagaagcg tgtgtttacc aaagctatat gacttaaaga aaaacagagt
     8641 tgatattgtg aaaaaaatca cttaatatag tataaggaga aaagggggat acaaatgaaa
     8701 aaattagttg ctggtttatt aacagccgtt gcacttcttg ctatgagcgc atgcggcgca
     8761 gatcagaaaa atacagcgca gacaagttct agtacgaagg aatctgccga agtatcatca
     8821 ggcgcttctg aacaagtata tacagatcca aaagaaatga aagaatctta tgatgtcgtg
     8881 attattggag ctggtggtgc agggatggca gcagctatcg aagcaaaaga tgctgggttg
     8941 aatccagtaa ttttggaaaa gatgcctgtt gccggaggaa acacatctaa atcatcaagc
     9001 ggtatgaatg cttcagaaac aaaatttcaa aaagaagaag gaatcaaaga ctcgaatgat
     9061 gccttctatg aagaaacgct aaaaggtggt ggaggaacga atgataaaga aatgcttcga
     9121 tactttgtcg atcactctgc tgatgcggtc gattggctag atcaaaacgg tatcaaactg
     9181 gacaacttaa cgatcactgg agggatgagt gaaaagagga cacatcgtcc gtctgatggc
     9241 tcggctattg gtggatactt agtggagggg ttattgaata acgtccatga gcgggaaatc
     9301 cctatttttg tcaacgcaga tgtgacagac attaagaaaa atggggaaac ggtcaatgaa
     9361 gtgactgtac atgttcaagg agaagaacca aaagaggtca ccggaaaagc aatcgtagtt
     9421 accacaggcg gttttggtgc cagcaaagaa tttattgaaa aatatcgtcc cgatttagct
     9481 gattatatta cgacaaatca agaaggatca actggagatg gtatcaccat gattgaaaaa
     9541 ttaggtggac aaaccgttga tatggataaa atacaaattc atccaactgt gcagcaggat
     9601 gagggggtct tgattggtga ggttgttcgc ggagaaggag caatcttggt cgatcaagag
     9661 gggaaaagat ttgtcaatga aatggataca agggataaag tatctgcagc aattacagca
     9721 ttgcctgaaa aatcagctta tttgattttt gaccaaggcg tgcgtgatcg tgccacagct
     9781 attgaatttt atgaccaaaa aggatatgtc acaactggaa agacaatgga agaattagca
     9841 gaaaaaatcg atttaccaaa agaaacacta gcaaaaacag ttgatggatg gaatacggat
     9901 gttgcggaga aaaaagatgc tcaattcaac cgtgccacag ccatggaaca tgacttatcg
     9961 tctccgaatt attatgcgat caaaatcgca ccaggaatcc attacacgat gggcggtgtg
    10021 aaaatcaata cgaacactga agtattagat aaagaaaacc agccaattag ggggttatat
    10081 gctgctggtg aagtcacagg tggcttacat ggaaacaacc ggatcggtgg aaattcagtt
    10141 gcggagatca ttatttttgg gcgacaagcg ggcatacaag cagctcgatt tgcttcggaa
    10201 aatgaatgaa atagaacgta aataaaaaga ggttgtgaaa tcagcgtttt gagctaatac
    10261 cgagggtcag ctgattgaac actgtttagt
//
LOCUS       DBNICI010000102        10247 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.103, whole genome shotgun sequence.
ACCESSION   DBNICI010000102 DBNICI010000000
VERSION     DBNICI010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.103"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(64..954)
                     /locus_tag="AC1ZCC_002005"
     CDS             complement(64..954)
                     /locus_tag="AC1ZCC_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIQ1630245.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            1152..2669
                     /locus_tag="AC1ZCC_002006"
     CDS             1152..2669
                     /locus_tag="AC1ZCC_002006"
                     /EC_number="3.2.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0031222 - arabinan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HIQ1630246.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            2641..3930
                     /locus_tag="AC1ZCC_002007"
     CDS             2641..3930
                     /locus_tag="AC1ZCC_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIQ1630247.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            4105..5001
                     /locus_tag="AC1ZCC_002008"
     CDS             4105..5001
                     /locus_tag="AC1ZCC_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1630248.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            5015..5878
                     /locus_tag="AC1ZCC_002009"
     CDS             5015..5878
                     /locus_tag="AC1ZCC_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1630249.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            5884..6448
                     /locus_tag="AC1ZCC_002010"
                     /pseudo
     CDS             5884..6448
                     /locus_tag="AC1ZCC_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            6470..7981
                     /locus_tag="AC1ZCC_002011"
     CDS             6470..7981
                     /locus_tag="AC1ZCC_002011"
                     /EC_number="3.2.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0031222 - arabinan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HIQ1630250.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            7993..8952
                     /locus_tag="AC1ZCC_002012"
     CDS             7993..8952
                     /locus_tag="AC1ZCC_002012"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HIQ1630251.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            9038..10117
                     /locus_tag="AC1ZCC_002013"
     CDS             9038..10117
                     /locus_tag="AC1ZCC_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIQ1630252.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
BASE COUNT         3300 a         1647 c         2120 g         3180 t
ORIGIN      
        1 aaaaacagcc gtcccttcat agggattggc tgtttttgtt ttataaaata gaaaagaaaa
       61 atatcagctg tagtaaactt ttaatttgat atcctgatcg taattaccga atcctttacc
      121 aaataaagta cagcccccca catgtttggc atcgtctttc acttcgattc gcaaatgcca
      181 agtgtcttgc tctttaggga tgtcgttgat cgtgatgttt gacaagggtt ctccattcat
      241 attcgtcaag tgtttggtaa ttcggatcgt cttcagcaag ccgtattgat taagattatc
      301 tggataccaa tcaggcgtat atttaccgcg gatatcagca aaatctccag ggctcgtcca
      361 tgttcctagc tccacaccat ttaggctgaa agtgatatct gaaggccaat tgtcatttga
      421 aaaaggaaat tcagaagaaa tttccacaga aacttccaac atctccaatg tatcctctgt
      481 atttaaaaaa ttaggtgcct gatactcgac aaatccgctg gtaaaccaga gaatgcgggc
      541 atccatcctt cgaggatcca taaaatagcg tggttcatcc actttcccga taaaatcatg
      601 gatcgtagct aaaccgcatg ttggttcaat tgcataattt gtatagtgac caataggtat
      661 cgatgtttct ttcgtatcaa aagcattgaa tattttttct ggaaaactga tatcgatttt
      721 atcgacacga aggctcgaaa ttttttgttg tcctactttt tctgttttga tgatgtttgc
      781 ttcctctagc tttttcacgt gcatcgtcgt gatagcactg ctcactccaa gttcttttgc
      841 caaatctttg acattcatct tttttttgga aagtagctgg atgatcttga tccttgtttt
      901 actagctaat gcttcatata ccggtaagga ttcttcgttt atttgtagtt gcatatatat
      961 cacctcttaa aaggtttttc ttatatatta acatatttgt taataaaagt tatattaaat
     1021 tatttgtttt aatcattatt aatattttgg ttaattaaaa tcgataaaaa ataagtaagt
     1081 ttaacattta tattattgac aacgctttca acgaggtgta ttattcatgt ataaattaaa
     1141 ggaggtcagt tatggaagcc acaatgttga ttagtagtgt caagcgtgta ggcaaagttg
     1201 atccgcggtt gtacagttca tttattgaac atatgggacg agcagtatat gaagggattt
     1261 atcagccaga tcatccatca gctaataaaa gtggatttcg ccaagacgtc attcacttgg
     1321 taaaagaatt gaacattccc atgattagat atcctggtgg aaattttgtg tcaggatttc
     1381 gttgggaaga cagtattggg cccatagcaa atagacctag aaggttggat ttagcttggc
     1441 ggacaattga aacgaatgaa gttgggattc atgaatttta taactggtgt cagcatgtgg
     1501 gggccgaaat caatatggct gtcaatctgg gtactcgtgg agtagatgca gctcgtaatt
     1561 tagtcgaata ttgtaatttt cctaaaggaa cttattggag tgatttaagg cgttcgaatg
     1621 gtcaagagag cccttttgga attaagacat ggtgtttagg gaatgaaatg gacggtcctt
     1681 ggcagattgg acataaaaca gcggaagaat atggacggtt agcagaggaa acggctaagg
     1741 cgatgaaact cgtagatgat tcaattgagg tggttttatg cggaagttcg aatcggcaaa
     1801 tgcctacatt tggtgattgg gagttaacag ttcttgatca agcgtacgat caaatagatt
     1861 atttgtctct gcatcaatat tatggcaacc aaaaaaatga tctttctcat tatcttgcac
     1921 gttccttaga catggatcgg ttcatttctg aagtgacagc gatgtgcgat acagtcaagg
     1981 cgaagaaaca tagtaagaag cagatcaacc tgtcctttga tgaatggaat atttggtatc
     2041 attcagcgga acaagatgag caagcagcgc cttggcagat tgctcctcct ttactagaag
     2101 ataactataa ttttgaagat gcattactat taggctgtct attaatcact ttattgaaac
     2161 atgcagatag agtaaagatt gcttgtcttg ctcaattggt aaatgttatt gcaccaatca
     2221 ggacagaaac aaacggagaa gcttggagac agacgatcta ttatccattt atgcaagctg
     2281 ctgtgtatgg acaaggagag gtgttaagcc cacaaattcg ttcagcaaac tatgcgacgg
     2341 aagattttga agaagtgccg tatttagaaa gtatagccgt aataaatgac aaagaacttg
     2401 tcatatttgc agttaaccgt ggagaagaag aaatgaatct aactgttcac ttgtctgaat
     2461 tatcattaga gggagtgatt gatttttctg aaatgagtgg atttgataca aaagcaacta
     2521 atcttcctaa aagtgaacaa gtaaaaccaa gtcattcaac aaatgtaaaa gctgaagatg
     2581 ggaaaatcaa ttgtttattg aaaccgctat cttggaatgt ggtcagatgt cgtttatcag
     2641 atgaaggagg aacaaaaaat gaaaggtaaa aaatggttaa ttggctcttt gttggtagct
     2701 gcaacaggta ttttaggagc ttgcggtggc ggaagttcag cagaatctga ttctgataaa
     2761 gaagtgactt tgactttttg gaatgggttt actgcatctg atggtgaaat attgcaagaa
     2821 atcgttaatg aatttaataa aacaaatgat aaaaacatca aaattgaaat ggatatcatg
     2881 acttgggcca atttgaatga aaaacttcct acagcgattt cttctaaaaa agcacctgac
     2941 tttattgcat taaattatcc agattttgca cagtatgtag aaaacggagc ggtacaacca
     3001 ttagacgatt tctgggaata tgatggagta gataaatctg attttaaaga aacggctatt
     3061 gaattaggtg aagtagaagg taaacagtat tttgtcccca tgcaggtaca aggcatgtat
     3121 atgtattgga ataatgattt gtttaaacaa gctggattag atactgaaca accacctact
     3181 acttgggaag aattgacaga aatggcacca aaattaactg atagtagtaa aaatgtatca
     3241 ggttttgtct tcaataagga tggaactgca ccgttatata actggatgtt agcaaatggc
     3301 ggtaaattag tgaacgatga ttatactaag tctgaatttg cgtctcctga gaaccttaaa
     3361 acgttaaaag cgatccaaaa aatgatccat gtagacaaga ctggtccaga atcgatttct
     3421 ggcgccgaaa tggacaattt aatgaatgct ggtcaactag ccattgaaat caatgggcca
     3481 tggttaaata acggattgaa agcaaacgag attgattata atgtgactac cttaccacaa
     3541 gggagtgatt cagacaaaaa aacagctatt ttagatggcg taggatatgc cattccggca
     3601 agtacagata ataataaaaa agaagctgtc tatgagtttt tgaaatattg gaatacaact
     3661 gaaattggta aaaaatggag tatcgaaaat ggttttcctg cctacttgga ttcagtagca
     3721 aaagatgaag aagtaaaaaa tgatcctatt gtatctgaat tatcgaaaca aatggactat
     3781 gcagagccat atttaccagg ttttacccaa atctctgcga tcaataacga tatcatcaat
     3841 ccactgattg aaaaattatt agcaggagaa gatcctgaga cactcatgaa cgatgcagat
     3901 gatgccatca atcaattact gcaaaaatag tacagaactt tatacaggat agactgttgg
     3961 caagatacag atatctgaat gatcgaccta gtatttgcag ataggtgtta ttgcaaaaat
     4021 cacagcgatt gttcggatat cttattcaga ttcgtttaaa ccaagattgt atattttatc
     4081 aaaatgaaag atgaggtgga ctatatgaac ggattcacca aatattggaa tcgtcctgat
     4141 cgtgctgcgt atttgttttt aactccatct tttttagttt tattattatt tactgtcata
     4201 ccgcttttag gaacatttgc tattagtttt acagatttga atattttctt tacatcaaga
     4261 aattttgtaa gttttgaaaa tttcacacgt atatttagtg atgaacgagc agttaattcg
     4321 ttgattcatt ctttatattt caccgtactc gaaacgccaa cacaaattat cgttggttta
     4381 ttagcagcag tagcattatc taaaaatacc agattcaatc gtttttgtcg ttcgacattt
     4441 tacattccag ttatttgttc attgacgtca attgcgatta ttttttctat gttgcttgat
     4501 ccgaatgtag gggcgattcc ttacttgttt tcacaagtcg gcttgccgat ccctcaattt
     4561 tttcgcgatc caacccttgc tatgccgacg gtagccatca tgactgtttg gaagaatttt
     4621 ggtgttacta tgacgattct cttgactgct attcagggaa tctctccctc tttgtatgaa
     4681 tctgctcaaa tggatggtgc aacaaatcga cagcagtttt ttaacattac gctacctcag
     4741 atcgttccct ccttaggttt ttgtatttta acgaatctga ttggttcgat gcaagtgttt
     4801 gaccaagtat atgttgcaac cgctggtggc ccacaattta agacagaaac agctgttcaa
     4861 tatatttaca gtcgaggatt tacagcacct tatgaattgg gctatgcatc agcactgtct
     4921 tcggttttgt ttatcattgt tgcaactata gctatctcca ctaatgtgta catgtcacgg
     4981 aaagaaaaac aaatgaaata ggagggaaac ctgtatggca ccagaaagct cgattacagt
     5041 tactgtgaat agtcgaaaag cagttagtgg taaaaaaatc attcgaacga ttagtatgct
     5101 agttatattg ttggttattg cgtttccttt tttgtggctg attatttctt cttttaaaca
     5161 cgaaaaagac attatctcat ttccgcctag aatatttgca gatagctata ccctagataa
     5221 ctatattaag gtttggacaa cgatcccttt attagattac atcaaaaata cagtgatttt
     5281 tgctggaggg acagtgatca cttctgtatt ctttgattct cttgcggggt atgcatttgc
     5341 tcgtatgcgt ttcaaaggaa aatcagtttt gttctatttc gttttgctga caatgatgat
     5401 cccattccaa gtcttcatga ttcctctatt tattgaagtc aatttgcttg gcatgcttga
     5461 tacttatgca ggtttgatca ttcctcgtat gacaactgca tttggtatct tcatgatgcg
     5521 ttcctttttt attactttac cagattcttt ggaagaggca gcacgcattg atggattaag
     5581 tgagtttaat atttttttga aaatcatgtt gccattgagc aaacctactc ttttatcatt
     5641 agggattttc acgctgatga atagttggaa tgacttgctt tatccattga tattgacaag
     5701 tagttcaaaa atgagaactt taccagcagg gttagcttta tttacagggc agaacatttc
     5761 tttttatgga cctgtaatgg ctggaacggt tatttcaatg ctgccattac tagtggttta
     5821 tatttttgca cagaaatatt ttgttcaagg aacagctatg tctggaatga aagagtgagg
     5881 aagatgaaaa tagttgagac taaactttat ggacagttga ttgtggtttc tgattacatc
     5941 ttattaggta ttctctgggt aatcgtgtgt ttgcctgtta ttactatcgt gcctgctaca
     6001 tgtgctgttt ttttatgtaa tggcgcaatg gaaaggtgag ggatcaggaa atatcattta
     6061 ttttttcttt caaggaataa agcaacattt tggtaagaac ttgttcctta gtctgttaac
     6121 gatcattatt tattttcttg ttaatcaatt attagaagaa cagacaatcg tactttcaat
     6181 tagtggatat gtagttttac ttatttattc catgtttctt ttatcctgga tatatgtgat
     6241 ttctgaaaac aaagatatca catttttaca aatttttgaa caaagtgcat ggaaagtatt
     6301 ttttgatttt ttgagaaata ttctttgttg cgtattgtta ttaggatttg tactgctcat
     6361 atttcttttc cctcctttta tttttatctt tgctggtggc gtatggaaac ttgtatctat
     6421 tatcttgact ataggaaatg aaaggtaggg aaagctgttg gacgaagaag tgaaagtaaa
     6481 aatcagagag gattttacgg taggagagat cagttctcgg ctgtttggct catttgttga
     6541 gcatatggga agtgtcgtct atacagggat ttatgaaccg ggccatgaaa cagctgatgg
     6601 tgacgggttt agacaagatg tcctctctta tgtaaaggaa ttagaattag gtgtcattcg
     6661 ctatcctgga ggaaacttta catctggtta tgattgggtg gatactgtag gtaggaaaga
     6721 acaacgacca aaaaaaattg atttagcatg gcgaggtata gaaccaaatt cttttggact
     6781 ccacgaattt tttaaatgga tcagtcatgt acaagcgaaa ccgatattca cggtgaattt
     6841 aggaacaaaa ggaattgatg aagcaaaaaa tttagtagaa tattgtaatt ttccaaaagg
     6901 aaccttctgg agtgatttaa gaataaaaaa tgggcaaaaa aagccttttg gcgttaaatt
     6961 atggtgttta gggaatgagc tagacggtcc atggcaagtg ggggctaaga gtgcttatga
     7021 atatggtagg ttagcaaatg aagcctcaaa agtcatgaag ttagtggatg attcaattga
     7081 aacaatctta gttggaagct ctacgccacg tttaccatcg taccctgaat gggatcgtat
     7141 cgcattagaa gaagcttatg agaatgtaga ttatttagct ttgcataact atattgataa
     7201 gtatgatgaa gaagacttga ctaaacctcc taaaaatgag cgtgatgata cagcgacata
     7261 ccttgcaaaa gcttatcgat tcgatcgtca aattgaagaa atcgttgcta cttgtgatta
     7321 tgtaaaagga tatttgagaa gtgagaaaac aatgtatcta gcgtttgatg aatggaatgt
     7381 acatgcccaa ccggaaaaaa gacataggga ttttgaagtc ggtagtccca tcgattggtg
     7441 ttactttact atggaagata cattattatt tggttcatta gggctggcta ttttacgaca
     7501 cgcagatcgc gtaaagatta gttgtcaatc attgttagta aacacaatac ctttaatact
     7561 gacagatgct aaaggacaag catggaggaa tccaacttat tatgtgatgc aacacttatc
     7621 gaaatatgct aaaggtcaag tattgaaaca acaaatcatt tgccctgttt atgattgtga
     7681 aaagataaaa aatgttcctt atgtggacag tgtgatcgta gataacggca atcagctgac
     7741 tattttcttg atcaatcgca caaatgaaca acaaaagata acgattgaac atgatttcaa
     7801 tttgctagat caaggagagc actacacatt aaccagtagc gatctgttgg acaaaaatac
     7861 acgtgaagaa ccagaaaaaa taaaacctga aataaactat ttttcaatag aaaaacatga
     7921 cgaattaaat gtagggatag aaaaatactc ttggaatgta attagaataa agaaaatgtg
     7981 aggaaatttg ttatgatgaa gacactttat aataatccta tcgttcggca acgggcagat
     8041 ccttgggtat ataaacatac agatggttat tattatttta caggatcggt tccaggctac
     8101 caagtgattg aattaagaag ggcaaagtca ctgaatgaat tagaacatgc tgcaagcctg
     8161 attgtatggc aggctcatga agaaggaccg atgagtgaat tgatctgggc acctgaagtc
     8221 cattatatca atggaaaatg gtatatttac tttgctgcaa gcaatcataa aactatacgt
     8281 gatgaatctc atcatcaccg catgttcgta cttgaaaatc agcatataga tccaatgaat
     8341 acagattgga tagaaaaggg acaaataaaa acgaaaaggg aaactttcag tttagacgga
     8401 acaactttct cttctcaagg aaagctatat tatgtgtggg cccaacaaga tcctgaaatt
     8461 actgggaatt ctaatcttta tctatccgaa atggataatc cttggacgtt aaaggggaag
     8521 cagatcctcc tttctatacc tgagtatgaa tgggaaaaac ggggtttttc agtaaatgaa
     8581 ggtccagcag tccttactcg caatgggaaa atattcatta cgtattctgg tagtgcaaca
     8641 gatgaaaact atgccatggg cctgctgtgg gctgatgagc acaagaatct tttattaaaa
     8701 gaatcatggc ataaattatc ggagccagta ttcgtttctt ctgaaaaaaa taagcaattt
     8761 ggtcctggtc acaactcgtt tacggtttca gaagacgggt gctttgatat tttgatctat
     8821 catgctcgac cagagaagaa ccaaaaaggg gatccgttag acaatcctaa tcggcacgcg
     8881 aacgcgcaat gctttacgtg gaatgagcaa ggatttccag aatttggtga acctgttccg
     8941 tatgccttat gagcacaaga aaccttgatt tttgcttgaa taaaccttgc agactcgtta
     9001 tgatatatgt acgtacaaat aaaaaggggc aaggcaaatg gcaaaatatc aagatattgc
     9061 agaagagata aaacaaaaga tccttcgtga ggaatacgaa gtaaatacaa ccattccctc
     9121 agaattcaag ctgcaagaaa tatatgacgt cagcagacat acgattcggc aagcaatcgc
     9181 tgtgttggta aatgagggct atctgcgtaa agaaaaaggg tcgggcacat atgtagatga
     9241 cagctataag aaagaaaaaa caccattgaa cagaacaaaa acgatcggtg tcatcaccac
     9301 atatctctct gattatattt ttccgtcgat tatccgagga atcgaaaatg aattacgaga
     9361 atctgggtat tcattgcttt tagccagtac gaataacgat cacgaattgg agaaagagtg
     9421 tttgaagaaa atgattgctt atggggtaga tggtttgatc gttgagccta cgaaaagtaa
     9481 tcagtataac ccaaacttag cactttatgt tgctttgaga gaacaaggca ttccgatggt
     9541 gatgatcaat gctgcttatg aagagttaaa tgcctcttat atttgtttag atgatgtgaa
     9601 agtcggtttt atggcagcag aatatttgat aaaaaaagga catcagcgtc tgttgttcat
     9661 cacaaaaatc gacgatcttc aaggaaaata caggatgaaa ggttttatca gcgcgtgtga
     9721 acagtataat gtgcgttttt tgacggaaga tatcattact ttcaccacgg aaacacgaga
     9781 aacaatcgct gaaaaaacag tacaacggtt gaaagaagaa caagctttag atggcatcat
     9841 ttgctataac gatcaaatag ctagtttaat gctagataag ttgacagaaa atggctatca
     9901 tattccagat gacctatcga tcgtaggaac agacgattct tctttaagcc atttagggaa
     9961 taaaaaattg acaacattga cgcatccaaa agaaaagttg ggacaagatg ccgcacggtg
    10021 gatcgtcaca acgattgaaa aaggacaggc acagccaaat attctctatg aacctgtact
    10081 agttgaacgg gaaacagtga aagaaaacga aaaataagag acgggttttt accgcctctt
    10141 attgctccta cggttactcg tcgcaatgtt tgtgagagct tttgtcatcg ctcaaggctg
    10201 aacgtttctt tcaccacctc tccactaaac ggtgttctcc taccaac
//
LOCUS       DBNICI010000103        10113 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.104, whole genome shotgun sequence.
ACCESSION   DBNICI010000103 DBNICI010000000
VERSION     DBNICI010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.104"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..1407
                     /locus_tag="AC1ZCC_002014"
     CDS             <1..1407
                     /locus_tag="AC1ZCC_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIQ1630253.1"
                     /translation="IPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVFFLLLGGL
                     LWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVGYLTMLYF
                     LTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGFVAGILLP
                     ALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVETSASHAN
                     EMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLEFLLRHID
                     PNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENTHGVPAHL
                     MIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKLVFKDDSL
                     ERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILIVSYLIVL
                     VIPFTIYACRHKFQHAEK"
     gene            1430..2356
                     /gene="glsA"
                     /locus_tag="AC1ZCC_002015"
     CDS             1430..2356
                     /gene="glsA"
                     /locus_tag="AC1ZCC_002015"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HIQ1630254.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            2564..4024
                     /locus_tag="AC1ZCC_002016"
     CDS             2564..4024
                     /locus_tag="AC1ZCC_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630255.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            4245..6008
                     /locus_tag="AC1ZCC_002017"
     CDS             4245..6008
                     /locus_tag="AC1ZCC_002017"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HIQ1630256.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            6092..6550
                     /locus_tag="AC1ZCC_002018"
     CDS             6092..6550
                     /locus_tag="AC1ZCC_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HIQ1630257.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            6659..7342
                     /locus_tag="AC1ZCC_002019"
     CDS             6659..7342
                     /locus_tag="AC1ZCC_002019"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047465 - N-acylglucosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HIQ1630258.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            7489..8103
                     /locus_tag="AC1ZCC_002020"
     CDS             7489..8103
                     /locus_tag="AC1ZCC_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIQ1630259.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            8119..8325
                     /locus_tag="AC1ZCC_002021"
     CDS             8119..8325
                     /locus_tag="AC1ZCC_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630260.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     regulatory      8550..8716
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            8890..9996
                     /locus_tag="AC1ZCC_002022"
     CDS             8890..9996
                     /locus_tag="AC1ZCC_002022"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIQ1630261.1"
                     /translation="MIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLSWSVIFLAR
                     SVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQFQPSELANL
                     FLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIAWVIFWAAA
                     VPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDSFIDSHGAG
                     YQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIGALCVLFLL
                     FSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGVPLPFVSYG
                     GTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
BASE COUNT         3337 a         1657 c         2074 g         3045 t
ORIGIN      
        1 attcctgcaa aaaaattaac gttctttggc tttttggcta tgaccatctc aatggtcgtt
       61 tcattatatg aatatcccac attcgcgacc tcaggcttca gcttagtatt tttcttgtta
      121 ttaggcggtt tgctatggtt tatccctgtg gcattgtgcg cagcagagat ggcaacggtg
      181 aaaggatggg aaaaaggcgg tgtctatact tgggtcagtc gcacattggg aaaacgattc
      241 ggttttgccg caatcttttt ccaatggttt gagataacag ttggctactt gaccatgctg
      301 tatttcttaa ctggtgcatt atcctatgca acgggtattg gtgctataca aaacaataaa
      361 tatctgaaat tggcaatcct attgattatt ttctgggcta ttttactctc acagttaaaa
      421 ggaaccaaat acacttcatt gattgctcgg atcggttttg tagctggtat cttgttgcca
      481 gcattagtct tgtttggctt agggatccat tatgtcgctt caggcgctcc actacaaacg
      541 atactgtcta tgaaaacatt gattcctgat ttttcgaaat tgcctacgct ggttgttttt
      601 gtttccttta ttttagcata tatgggcgta gaaacatctg ccagtcatgc aaatgaaatg
      661 gaaaacccta aaaaggatta tccattagca atgtttgtat tggttattct ggcaatcatt
      721 ttagatactt ttggcggatt gaccgtcgct actacagtac ctcaacatgg tttgtctctt
      781 aatacagggg tgattcaatc gttagaattc ctattaagac acattgatcc aaatcttgca
      841 tggattgcga aaattcttgc gattcttgtt tgtttaggtg tcatcggaga aattgcttct
      901 tgggtaacct ctccttcaaa agcacttcac gtagcagcag aagaaggatt acttcctgaa
      961 tattttgcaa aagaaaacac acacggtgtt cctgctcatt tgatgatcgc aaatgggatc
     1021 gttgcaacaa tctgggcagc cgtctttaca ttaagcggcg gggggaataa tctttcattt
     1081 cttgcagcga tgtcattgac tgttgtaatc tacctgatgt tgtactttat gtttttcgcg
     1141 ggctatttga agctggtttt taaagatgac agtcttgaaa gagcctatca ggttccaggc
     1201 ggaaaagttg taaaagtaat cgttgcttgt ttaggtcttt tgacttcact ttttgctttt
     1261 tgtatctcat ttgttccacc tgctagtatc gcagctggac agcatgggat ttatgaaacg
     1321 attctgattg tttcttatct tatcgtctta gttattccat ttacgatcta cgcatgccgt
     1381 cataaattcc agcatgcaga aaaataaaaa atgaaagctg gttattcaaa tggaagtaaa
     1441 agaattagaa caattgattg gaaaaaattt ccactattat caaacaggac aagtggcgga
     1501 ttacattcca gcattagctg aagtggatcc caaacaatta ggtatggcga tttatgattt
     1561 ggatacaaaa caactgattg agggaggaga cgccgatgtg cgctttgcca tcgaaagcat
     1621 gtcaaaagtt cccgtattgt tattagcaat caaagacaac ggaatagaaa aagtctttca
     1681 aacaatcaac acggaaccaa ccggttttgc cttcaattct atcatgaata tggaaatcaa
     1741 tcatcgcaaa catccaatga atccttttgt taatgcagga gcgattgcaa caacctcgtt
     1801 gattgccggt aaagatgcag atgaaaaatt tgagcgaatc ttgtcattta tgaaagagat
     1861 ctgtgatgat cctaatatca cattgaatga agaaatctat cattctgaat caagaacagg
     1921 tgatatcaat cgctctcttg cttactatat gaaaggtaat caaatgatcg aaggagacgt
     1981 accggagata ctcgatgctt atttcagaca atgctccctc aatgtcaccg ctacaggcat
     2041 agcaaaatta gctgctgtgc tggcaaataa aggaattgct ccttggaatg gcaaacaaat
     2101 cattactgaa gaaagtgcta caatcgtcaa atcaattatg acaactgctg gtttgtatga
     2161 tgaatcagga gagttttccg tccatgttgg tgtacctgca aaaagcggtg taggcggcgg
     2221 gttgatggca gctgtgccaa atcgttacgg cataggtgtc ttcagcccgg cactagatcc
     2281 atttggtaac agcgcagctg gtatccaatt gttgaaagat gtagtcaaag aactagacgc
     2341 tgacatcttt gaataaccat tcttgaaaga ggctggggta aaagtccaaa cggctcaaag
     2401 ctacctacaa tgaggctgaa attgtgagct tgtttgactt ttgtcccagt ttcttttttt
     2461 attggttttt gaaagggctt ttctaaataa aaaggaaaca aaaatacgat ttacagatat
     2521 gattaaataa aatcataaga aaaactggag gcgtgtatca ggaatgctgg aaaattacat
     2581 cgagcgaaat atatttagaa aagtgtatct gtgtgaacaa ttatttgaat tccaagaaat
     2641 agacatcgaa caaacagcta tttctctaag ggtgactaca ccaacgatcc ttcacgatct
     2701 agaaagtcta gccgagtgtc tagaatattg tataaaagaa caagtaagag agaagcataa
     2761 gtataaactc gtattcaaac atggtattgc tctttctgag ctcacacagt ttctgtacgg
     2821 tcaatcttat tttttaaagt ttttaagtta ttattttaat ggtatattca cgagtacaga
     2881 actggctgat cttgaattta tttctttgag caaagtatat acaatcaaaa aaatcgtatt
     2941 ggattttttt aaagaaaatg attatttgaa aaataaagaa atcattatcc cagaatttga
     3001 ctcgcggaac atcttgctag ctttagtacg ttatgtcaat tgggaaggct atgaaaataa
     3061 aaatccgtat gtagaagaag cttgtaacga gttgattgat tatgtagaga ctcatttttt
     3121 taaaagaaga tattcggaag atgaaaagtc ctttatcata cgaggaatag agatcgcaat
     3181 cggaagaaag gcatttcccc ttggactgaa agaagaagat aagaaaagcg cagaaacgaa
     3241 gccgttattt caattagtct gtcaaggatt ggaagagcaa aaaggaattc tagacttaca
     3301 agaagaagat gtctattata ttttttcttt gttcaacagt agaaattata ccaatgagaa
     3361 catggagctg ttaagaaaag atttcgaagt tgtttataaa aattttgttc tcaatgacct
     3421 atcactgaat gaattgattt tacagattat tagtatcgct gaaatcgacg atgcagaaaa
     3481 ttttattttc aagcaatcct ttttacaatt tgtccgtacg ttgtgggcag atggacaagt
     3541 atttttaccg gaaaggatct atcttctatc agaaaaagaa caacttctgt acggaaaaat
     3601 tgtagaaata ttggaagagt ggaaaaagaa aaatcaaatc caactaagat ggaatgaaaa
     3661 tttcattcga aaattcgtca aaagtctttc tttgatcaac agtgaacaag cggggagaca
     3721 ggaaatagaa atttttgttg tttcagaaag ttcagtcaaa caattttttt atcgaaccca
     3781 atttcatttg tatgcggaag aaggcatcgc tgaaataaat cctattttat ttcgttcatt
     3841 agaagaattg cctgatgaat gtctatgtgc gaaaaaaaga gtggttttgt gcgatgctgc
     3901 tagctatcaa aatggattag aaactgaaaa gacaaagatc tatccaatct cattaaaagg
     3961 tgcaaacatc catttgtatc atattgctca ggaacttcac ctattaaaaa aacatatttc
     4021 ttgatgagac cttaattagg agaccttcta caaagctttt taattgtctg tagaaggttt
     4081 ttatttgagt cctgattcgt gcgaagatct acttatataa caagctttct ctgaaatttt
     4141 tttaaaatgc tattgactta gagtcaactc taagtagtag aatagataaa gtgtaacaaa
     4201 tctattttca ttaaaagaag atagtacgga ggagagaatg tattatgagt acagtattac
     4261 atgtattgga tgcaatcaat agccccgcag atgtcaaaaa attatctgtt gaggaaatga
     4321 aacagttggc agaagagatg cgccaaaaaa tcttgcaaaa ggacagcgat gtaggtgggc
     4381 acgttggtcc gaatctaggt gtagtagaat taacgattgc gtttcattat gtatttgatt
     4441 caccaaagga taaaatcgtt tgggatgttt cccatcagtc ttatggtcat aagcttttaa
     4501 ctggtcgaaa aaatggtttt gaaaaaggac attaccgtga agtgagcgga tattccaacc
     4561 aacatgaaag taaacatgac tattttacag tggggcacac atctacatct atcagcttag
     4621 caacaggttt agctaaagcg agagatctga agaaagaatc aggaaatgtg gtagcattta
     4681 tcggtgacgg ctccctaagt ggtggcttag cttttgaagg attgaacaat ggtggtgttt
     4741 tacaatccaa tctaatcgta ttagtaaacg ataatgagat gtctattgat gagaatcatg
     4801 gcagtttata taaaaactta gctgagcttc gtcaaaccaa cggtaaaagc agcaataacc
     4861 tatttaaagc aatgggattt gattatcgtt acgtggataa tggaaacgat ctggaaacgg
     4921 tgatccgttt atttgaagaa gtaaaagata ttgatcatcc gattgtactt cacgtccata
     4981 cggaaaaagg tcgcggttat cagccggcaa tcgataataa aatgaaatat cattggcatg
     5041 taccatttga tctgtccacc ggggaggcaa agcaagtaac ttctggagaa acatttaata
     5101 tgatcatcat ggattatttg gataagaaaa tgcaaacaag tacagctcct attgccgcag
     5161 tcaatgcagc gataccaggc gcctttggac tgaaaacatt ccaagaaaaa catccagatc
     5221 attattttga cgtggggatc gctgaacagc actctatcac tttcgcttct gctttggctt
     5281 ctcagggagt acgtccagtc gtcttccaat caagtacttt cttgcaacgg gcatatgatc
     5341 aattatcaca tgatttagcg atcaacgaga atccagtgat tattttagtc ggtggcggaa
     5401 cgatctcagc tagtgaccga acacaccaag gaacatttga cattccttac ttatcaagta
     5461 tcccgaacat caaatatctt gctccagcaa caaaagagga tatgatctct atgctagatt
     5521 gggcgttcga acaaacagcg cagcctgtag caatccgaat tcctacacat ggtgtagaac
     5581 atggtacagc tatcgcaaat gattatagta ttccagccta tcaaacagta aataatggag
     5641 gaaaagtagc aattttagct ttaggcggtt ttcttgaatt aggaaaagaa acagtaaaag
     5701 cttttagaaa aaaaggaatc gaagcaacgt tgatcaatcc aggaacgatc acagaaaatg
     5761 acgtagaaac attagataaa ttaaaagaaa cacacgaatt agtcatcaca ttagaagatg
     5821 gtagtttgtc aggaggattt ggcgaaaata tcagtcgtta ttatggtgat tcttcaatga
     5881 acgtattaaa ttttggtgca gagaaagaat tcacggatag cgttcctgta gggcagcttt
     5941 atgaacgtta tcatttaaca ccaaaacaaa tcgttgaaga tagtctgcgt gtcttgaata
     6001 ttcaataatt cattatgctc accatccaga taattgttta tactagtatc tggatggttt
     6061 tttcattata aaaagcaaag gggtggatgt tatgtatcag caagtagaag aatatgtcaa
     6121 aaagttccaa gaaaaatatc ctagttatca atcagaaaaa ttaggtttct tttcatttgg
     6181 ggatacagaa aaaatggcag acgagttgag cgaactagtt gtaaaaaaga taaagactgg
     6241 tacatcaagt ggttacgatc tgtataaaca agagaatgag cctttaccaa aagttggtca
     6301 attagacgtg gtcctgaatg gaaaaaatga accagtttgt atcatcaaaa atataaatgt
     6361 gaccatcttg ccttttagtg aagtcacgaa agagcatgct tggaaagaag gagaagggga
     6421 tcggacatta gcttattggc ggaaagtcca tgctgagttt tttaaacatg cttataaaga
     6481 agcgttgaac atagaattta cagaagagaa gctggtcgta tacgaagaat ttgaagtaat
     6541 tttcttttag taagaaaggt ctgctgaaag aaatagctca ttattgtctc ctgaatagaa
     6601 aagggttaca tttttatatg aagctgtatt tttatataac attactggag gagatattgt
     6661 ggatacactg gaacaaatca aaggcggagt cgtcgtttct tgtcaagctt tggaaaacga
     6721 gccgctacac agttctttta ttatggggag aatggctttg gctgcaaaag aaggcggagc
     6781 agttggtatt cgagcaaata cgacagaaga tatcaatgaa atcaagcgaa tcgttgattt
     6841 accggtaata gggattatca aaagagatta cgatgggtca gaagtgttta ttactgcgac
     6901 agaaaaagaa gtggaagaac ttttggagac gcaagcagaa atcattgcac tggatgcaac
     6961 ttctcgtaaa cgtccgacag aaaaaacaac tgctgaactt gtccaaatga ttcacgaaca
     7021 cggtcggtta gctatggcag atatttcgac ttttgaggaa gcgcttcaag cgcaagaaga
     7081 tggttttgat cttttgtcta cgactttagc aggctataca gaatattcac gaaagacgca
     7141 gacacctgat tttgatttat tgaatcaaat catagaacaa gtcgatatcc cagtaattat
     7201 ggaaggacat actgactctc ctgaacaagt agagaaagca tatgaattag gtgctttttc
     7261 agtcgtagtt ggatctatta tcacccgtcc gcaattgatc actaaaagat atgtcgaagc
     7321 cgcaagtaaa gcaaataact aaaaaacacg cacggcacag attcttgaca aaaaagaaaa
     7381 attgatagat agtttaaagc agattctaag aagatgaaaa aagcattact tttcattttg
     7441 tcttcaagat ctgcttttta acgaatttta tagaaagaag gcgaaaccat gtttgaacgg
     7501 agtccttcag aattgacaga acgtgaaaat tacaagttgc tgattggttc agtcatccct
     7561 agaccagtag cagtagtaac gactaaatca gcaaaagata tagtgaatat cgcacccttc
     7621 agttacttca atatcgtcag ttcgaatcga cccatcttat ccttggcgat ccagagaaaa
     7681 caagatgcag taaaagacac agcaaagaat attcttgaga caagagaagc tgttatccat
     7741 atccttgatg aggacaacgt agaagaagta aataaaacag cagccaatct gcctaatgag
     7801 gaaagcgaac tgtacctgac tgacttgact ttaactgatt cagtaatcat ttcagtacca
     7861 gcattgacag aagcaaaagt tcgttttgaa acatccttat atgaacatgt cgagatacgg
     7921 aataaaaata aagtaacagc tgatttattt ttgctagaag tacagaaata tcatttaaat
     7981 gaaacagtct atgatgataa gacaggaagg atcgatccaa gaaaattgaa tgctgtaagc
     8041 cgtttggcag gaaatgatta tgcagcaata ggagaaattt tcacgattgc acgtcccgaa
     8101 taaaaaggag cactagttat gttaaaaata agctataaac catcgacaga ttcaaaagaa
     8161 atgaaaaaag aatatgaaac agtgaatgat tttctgcaag ggcagtactt agaagttccg
     8221 ccattgcaag accattttgt cgttactact gttactttag atggtaagga aatagaaatg
     8281 ccagatcaaa cgattagcgg attgtttaat tactttaaca aataaatatt tattccgaaa
     8341 agtaaaaaaa catataaaaa aacacataaa actaaaaaat tatcaaagaa tcctggtgca
     8401 actgaaagaa tttgcaacgg gattctttca acttatttgt tatttttttc taaaaaatat
     8461 taaagattgt taaatttcac tatttcattt caaattccat tgcctaatca ttaggaaaag
     8521 tctatcatga actcgtaaat agaatatagt aataaccttg ttaggtgagg ctcctataca
     8581 aacataggct gttgcccaga aacgtcgaaa gtcgccaatg ggtaaaacag aaactgttgg
     8641 attaaggctt tttccaatag ctaagaatgt tttctttacg ttgtatagtg ctaaaactcg
     8701 gacgatgaga tgatgctagg tcagaattct tgttcgaaaa ggtttctgac cttttcttta
     8761 tatttttgtg actgacttat ttgatgagac aaactcgctc ctcacctgac aagtggattg
     8821 ctgtgtgagg aggagatgcg gtgaaaaaag atcgattggt ggattttggg tccttatttg
     8881 actttgtcca tgatcggtct tttagaagta tacagtgcca gttcttatcg tttattgcaa
     8941 gccgatgaaa atacaaaaag tctattattg cgtcaactaa ttttcatatt tttgagttgg
     9001 agcgtgatct tcttagctcg ttcagtcaaa ctacactatt tacttcaccc taaaatagca
     9061 ggatacggtt tagccttatc gattttcttt ttagtattag taagaatagg gatattcggt
     9121 gtcactgtca acggcgcaca acgttggatc tctctgtttg gcattcaatt ccagccttct
     9181 gaactggcaa atcttttttt gattttttat ttaagctggt tttttcgtga cggaaatagt
     9241 agcccaaaag atctaaaaaa accattcctg attacagtag gtataacttt tctgatttta
     9301 tttcagccaa agattgctgg agcattgatg atcctttcga ttgcgtgggt catattttgg
     9361 gcagcggcgg ttccatttaa aaaagggatc tatctaatcg ttactttttc tgcattgctg
     9421 attggagcag caggcggggt attatattta ggaaataaag gttggcttcc acagatgttt
     9481 aatcatgctt atgaaaggat cgcgacttta agagattctt ttatcgatag tcatggtgcg
     9541 ggctatcaaa tgacccattc gttttatgca ctatataatg gtggcatatt tgggcgggga
     9601 ttaggaaata gtatcacaaa aaaagggtat cttccagaga cagaaacaga ttttattttt
     9661 tcgattatta cagaagagct tgggctaatc ggtgcattgt gtgtgctttt cttattgttc
     9721 tcattatgca tgagaatctt ttgtttaagc agtcgctgta aaaatcagca agctggattg
     9781 tttcttttag gattcggaac tttgctattc gttcagacga taatgaacgt gggaagtatc
     9841 gcggggctga tgccaatgac tggtgtgcca ttgccatttg ttagctacgg aggaacaagt
     9901 tatctgatcc tttctttagg aatcggaatc acgttgaata tttcttcaaa gatacaggcc
     9961 gaagagttgg cgctctatcg acctgaaaaa caataaagag aaactgagaa actctcagtt
    10021 tgaattatca taaagtacaa tcttttattt ttcaagcgag aggttctttt agaaagagga
    10081 attcgcaatg aaagtatctc aaaaaatcat att
//
LOCUS       DBNICI010000104         9948 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.105, whole genome shotgun sequence.
ACCESSION   DBNICI010000104 DBNICI010000000
VERSION     DBNICI010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.105"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="AC1ZCC_002023"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="AC1ZCC_002023"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HIQ1630262.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="AC1ZCC_002024"
     CDS             complement(1173..1868)
                     /locus_tag="AC1ZCC_002024"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HIQ1630263.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="AC1ZCC_002025"
     CDS             complement(1881..2597)
                     /locus_tag="AC1ZCC_002025"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HIQ1630264.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3532
                     /locus_tag="AC1ZCC_002026"
     CDS             2927..3532
                     /locus_tag="AC1ZCC_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HIQ1630265.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3857..4180
                     /locus_tag="AC1ZCC_002027"
     CDS             3857..4180
                     /locus_tag="AC1ZCC_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HIQ1630266.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4362..5732
                     /locus_tag="AC1ZCC_002028"
     CDS             4362..5732
                     /locus_tag="AC1ZCC_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIQ1630267.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5862..7163)
                     /locus_tag="AC1ZCC_002029"
     CDS             complement(5862..7163)
                     /locus_tag="AC1ZCC_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIQ1630268.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7393..9384)
                     /locus_tag="AC1ZCC_002030"
     CDS             complement(7393..9384)
                     /locus_tag="AC1ZCC_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HIQ1630269.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9528..9794)
                     /locus_tag="AC1ZCC_002031"
     CDS             complement(9528..9794)
                     /locus_tag="AC1ZCC_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIQ1630270.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
BASE COUNT         2912 a         2068 c         1804 g         3164 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 aagcacaggt gaaggtggcg aaccagaagt gatcggttca acagaagaat aatactgaaa
     3541 caatcaggtg gtgttagaag cggcgggtca gcagctttta acacgccttt tttatttcta
     3601 atcgtttatt ttcatatggg gcttccactt ttgtaaatgg ctttacaaac gaaaaatcct
     3661 aatttaccaa gcttttcctc gcatactttt atcgtacctt tataaaaatt tatactttga
     3721 aaaaaagtat tcaattattg ttgcattttt cctgattctt tattagaatg aaccctcgtt
     3781 gaagtaagta tttctagttt atcctaggat taattataaa aataaaacat tcgttgattc
     3841 gaaggaggtt aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt acgcttcagt
     3901 tggtgtcgta tcaagtttgc cagatgaatt gattgacagt atatggttta ttattgacct
     3961 agatcttaag ggagtcattc cattagaaaa tatattaacc tttgatttga tcgaaaatcg
     4021 tggaaaagtg acgatgcatt tttctcaagc aggaagcaat gtggaaatgg atattgattt
     4081 gccgtttgcc tattcgaacc aatttcctgc tcaagtatac gcctatgatg acggtacgag
     4141 agaaacgatc cttttaccaa gtgagattag acaatattaa ggtgatagat aaacgtggat
     4201 gtcccttatt ttacagacca ggccaagttg ttgattcttt gactcaattc ttggcttctt
     4261 tttatggagt ttcataaatt atcattgatt tctaattggt attctcagga taaacgctat
     4321 atactactaa ctaaatttta aagatataag gagtttaaaa gatgcgcgat ttaacaaaag
     4381 gaagccccgc taaactgatt ttgatgttca ctgttccttt actgatcgga aatgtttttc
     4441 agcagttcta caatatgatc gatatgatca tcgtgggtca gacgctaggt aaaaatgcgc
     4501 tggcagcggt tggtgcaact ggaagcctga catttttgat cattggcttt gcccaaggtt
     4561 taacagctgg tttagccatt attaccgcac aacgttacgg tgcaaaagat tatcggggtc
     4621 tgaagaaaag ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg attttaactg
     4681 tcctaagtct cctgtttatt cgtccgatgc ttcaattgat gcaaactcct cctgaaatac
     4741 ttgatcaagc acaaacattt atttcaatta ttttattagg gatctttgca tcagtcagtt
     4801 tcaatttgct ttctaatgtc attcgcgcac ttggtgacag ccgcacacca ctattctttc
     4861 tgatcattgc ggtcatcatc aacgtcgtac ttgatttgat ctttattatc tattttggca
     4921 tgggtgtgga aggtgcagct attgctacag taatcgcaca agtttcttcc agcgtactgt
     4981 gtctagtgta tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa gatttcaaat
     5041 ttgaaaaaga agagtttgct gtacacctaa atgctgcttt gccaatggcg tttcaatctt
     5101 cgatcatcgc tgttggtgct atcgtgttac aagcagcact aaatagtcta gggacagatg
     5161 tcgtagctgc tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag ccgatgatgt
     5221 catttggtat tgcaatggca actttcactg cacaaaacta tggtgcgaaa gaatatggcc
     5281 gaatcttgca aggggtacgt caaactttac tgatgagtgt cggatttagt ctcttagcag
     5341 gtgctatcgt cattttcttc ggtcatttct ttgttcaatt gtttgttagc ccatcagaaa
     5401 cacgtgtatt tgaattagca caaacctact tcaatatcaa tgggtctctt tactggatct
     5461 tagcagtcct gttcatttta cgctacacac ttcaagggct tggacaaagc aagatcccta
     5521 cgatcgccgg tatgatggaa ttgcttatgc gttcttttgc agctatcgtt cttacaggaa
     5581 tgtggggata cccaggagca gcagcagctt ctcctttagc ttggatcggt tctgttgccg
     5641 tgttgcttta ctcttatatg cgttcgatga aacaattgaa acacatgggt gaagaggaac
     5701 atttcacatt agaacatgca gaagcaagat agccttgatt aaaagaaatc gttgcggcta
     5761 aaaatgaaaa cttttattga aaatatcttc ttcgtttacc gaaaacaggt tttttagata
     5821 acaaaaaggc tgtgacaaaa aatgtcacag ccttttttct tttattctat ttttctttcc
     5881 gtggactcta ctttgaaata atcgatatgt gcatgctggt attctaatac cgtgaaacgg
     5941 aatgatgcaa tctcaatgat tgttcctgct tttatttctt tatatcggct agaagcaaag
     6001 cccgcaagtg tagtaaaatt cgtttcttca aactctggaa tctctacatg aaaataacgc
     6061 tggaaatcat ctaatgttag tttgccggag atgatataag agccatctga ctgttccgct
     6121 atcaatgctt cgtccaaatc tgtctcatct cggatttccc cgacgatttc ttctaaaata
     6181 tcttcaatcg tcactaagcc gcttgtccct ccgtattcat cttttaagat agcaatatgc
     6241 tttccctttt ttcgcatgat cgtcaataat ttttttatag ggatcgtttc agttgttacg
     6301 atcggctctt caatcaactg ttctactgtc agctggtcat ctttataaga ttgcttgatc
     6361 aaatctggca aggtgacata acctaaaaca ttgtcttttg attctaagat aaccggatag
     6421 cgagtatgtc cttgttcaat cgaagctttt atcgcttctt tgacggtttc tgaagcttcg
     6481 aatacttgca tggacgtacg gtctacttgt atatcttttg ccaaggtatc atcaaattca
     6541 aacacgtttt ccaaataatg atattcttct ttggtcaact ctcctttttc ataagaattc
     6601 aatgcgacta atgtcagctc ttcttgagac aatgtctctt ctccttcaga tacgagacgc
     6661 acacctaata gcttccctag ccctgcagct gaatgattca atacccaaat aaaaggaaac
     6721 attactttat aaaaataatg aagtggttta actacgaaca agacgacctt ctccgtattg
     6781 acgatactat agctcttagg aagcagttcg ccaataacca catcgatata cgtaactaaa
     6841 ataaatccca gcgccacaga gattgtactg ctaatagccg aactaaaagg tagtttaccg
     6901 atcactggtt ctagtaaagc cgcgacagat ccttcaccga cccacccgat gatcaaaccg
     6961 gttagcgtaa ttcctaattg actagctgat aaatagttat ctaaatggtg tgtaacatga
     7021 agagctagct tggcgttctt tacattttcc ttttctagtt gttccaatct tgatcgtctg
     7081 attttcacta aagcaaattc acttgctaca aaaaatgaag taattgcaat catcaaaaat
     7141 aaaataatga aattgataac catattctcc cgcctttctc tttttctatt gtaccctttt
     7201 tctacaaaaa gattgaaaat aatgcgtatt catatttagg aataaaaaag gagatgcggc
     7261 atttttcagc cacatctcct tatcacttca aaatcattgc gttacttgtt gctaaacagc
     7321 tttttcatga cctctgataa acggtgttca agtagctgac attcgacatt agttcaggtc
     7381 aaagcgctac tttcacaacc gctctttcaa ccatgttttc cgagtatttc caatgataag
     7441 cggaacagtt tcatgtttta cttcgctgta ctctagccca aaccatgttt ctggcgaggt
     7501 agcgattttt ttgattgcca gttttgcctg catgatttga cgttgccatt ttcctagagt
     7561 tgtcacattg gaaactactt catcaatcag gacaaattgg aaatcgccaa cttctcgccc
     7621 tggtgtcaga gagtatcttt gtggctgttt tggcaaacgg ccttgtttca tcaaatcatg
     7681 gatgacttgg cgaacatata cgttgatttc ctgttggacc ctgaaaccta agaacagatt
     7741 cacttggaca ataaaatcag tgtccatcat atctacttga tattctttcg tatatggctc
     7801 atccgtcact tccacatgaa caaaccaata gactcgtgct cttttaggcc gtttatctaa
     7861 aatcgaatag atgaactgtc ggccaacttt atcttcagcc atatcaggaa ccaagaagac
     7921 cacatttgtc tgatagattg gtaaagaagt atcatcacgt aattctctaa gctgcgggat
     7981 ataatctctc aaagacacat cctcggttga cgattcgcga ataatgttcc cttgttccca
     8041 aataaacatc acgactaaga tgatcaaacc aatcagaacg gctacaaaac cgcctcggaa
     8101 gaatttgaca acacttgaga taaagaaaac gacttctatc gcaccgaaga aaaaggtaac
     8161 taaatatgca agccaacgag aatgcccatt ttggataaga taaaagtaaa gcagtgctgt
     8221 tgtcatcaac atcgtcacag tgatcgatag accgtaagca gcttccatat gatgagacgt
     8281 tcggaatgtg acaaccacta gagaacaggc gatccataaa atcagattga ctgctgggat
     8341 atacatttgc ccgatactgt tcccaggata catgatcttt aaccgaggca gcagtttcaa
     8401 cttgattgct tctgaaacta gcgtaaacga acctgtaatc aatgcttgcg aagcaatgac
     8461 tgcagcgact gttgcaaaag caacgccgat cacgatccaa ctttgaggca tcatttggaa
     8521 gaatggattc aggttcttga tttgctgtgc ttcagggttt tgataaacat tcaacaacca
     8581 cgcagcctgc ccaaaataat tgataaccaa acaaattttg acgtaaggcc agctcagacg
     8641 aatatttcgt ttgccgacat gtccaagatc ggaataaagc gcctctgcac ctgtcgtagc
     8701 caggaaaata tttccaagaa tgaacaagcc taatttgtta tctgcactaa aaagcagctg
     8761 gatagcataa taaggattca gtgcacggat aactgtccag tcttgactga agttcattaa
     8821 tcccattacg cctaaaaaag taaaccaagc gaacatgatt ggtccgaatg cttttccgac
     8881 aatatctgta ccaaagcgtt ggaccatgaa taaaacaaaa ataataacta aagtgataat
     8941 gacgatcacg ttctggttat taccgaaccg gtcaaaaaac tgcggaattc ctcgcaagcc
     9001 ttcaatcgca gtcgttaccg tcacagcagg ggtcaatacc ccatctgcta gtaaggcagc
     9061 tcctccgatc attgcaggaa tgatcaaata tttcccacct tttcggacaa gtgtgtagag
     9121 tgagaaaatt ccaccttcac catggttatc tgcattcaat gcaatcacga catatttgat
     9181 tgttgtcagt agagtcaatg tccaaaaaat caatgagact gatccgataa caaaatcagg
     9241 attgattcct gacaatccgc cattatcttc aacaatcgat ttcataacgt aaagcggact
     9301 tgtcccgatg tctccgtaga cgactcccat cgctacaagc aggccagcca ttgatatctt
     9361 tttggtattg tgcgttgttg ccacgttttt ccctcatttc ttgttatatg gatatccttt
     9421 aggtaagctt gaatgataga tgatattctt atccatgaca cccgctttcc taaattgctt
     9481 ctaattatcc aagtaacaca ctgaagtgtc aacttatttg aataaaatta ttctttcttt
     9541 ggacgttctc tgatacgtct tgccacaaat gaataaagag caaggcaaaa aattgcaccc
     9601 aataatgctg gtatcaaata aaaaccgccg agttccggtc ccagtggtcc tagtaaaaat
     9661 tcgccgagcc aactacctaa aaaaccgata atgatatttc cagatacgcc tcccgggact
     9721 tcctttccta aaattgcgcc agctatcaaa cctaatatac cgccgataaa taacgaccat
     9781 aaaaaattca gcatctcctt tccctccttt atccatattt ctcactttta ttttacgctt
     9841 tattcagaag gactccaaaa atatgcgcca agaaactcca gaaaaaaaga agttgtaaca
     9901 aaagtgttca gcttatttcc gtagaagctg cttctggaac accgtgaa
//
LOCUS       DBNICI010000105         9813 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.106, whole genome shotgun sequence.
ACCESSION   DBNICI010000105 DBNICI010000000
VERSION     DBNICI010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.106"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(34..2154)
                     /locus_tag="AC1ZCC_002032"
     CDS             complement(34..2154)
                     /locus_tag="AC1ZCC_002032"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIQ1630271.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2384..3562
                     /locus_tag="AC1ZCC_002033"
     CDS             2384..3562
                     /locus_tag="AC1ZCC_002033"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HIQ1630272.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3578..4336
                     /gene="aroD"
                     /locus_tag="AC1ZCC_002034"
     CDS             3578..4336
                     /gene="aroD"
                     /locus_tag="AC1ZCC_002034"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HIQ1630273.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4359..4844
                     /gene="ybaK"
                     /locus_tag="AC1ZCC_002035"
     CDS             4359..4844
                     /gene="ybaK"
                     /locus_tag="AC1ZCC_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HIQ1630274.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4915..6168)
                     /locus_tag="AC1ZCC_002036"
     CDS             complement(4915..6168)
                     /locus_tag="AC1ZCC_002036"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIQ1630275.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6285..7634
                     /gene="gdhA"
                     /locus_tag="AC1ZCC_002037"
     CDS             6285..7634
                     /gene="gdhA"
                     /locus_tag="AC1ZCC_002037"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HIQ1630276.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7746..9092
                     /locus_tag="AC1ZCC_002038"
     CDS             7746..9092
                     /locus_tag="AC1ZCC_002038"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HIQ1630277.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9400..9786)
                     /locus_tag="AC1ZCC_002039"
     CDS             complement(9400..9786)
                     /locus_tag="AC1ZCC_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HIQ1630278.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3128 a         1782 c         2011 g         2892 t
ORIGIN      
        1 aattttgtcg ctgctccttt taaatttatt ttatcattcg gaagaacttg tggtcgttcc
       61 gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc
      121 actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa
      181 gccattcttg tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa
      241 ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc
      301 tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt
      361 ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc
      421 acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata
      481 attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc
      541 gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg
      601 ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc
      661 tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga
      721 attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa
      781 ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa
      841 tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag caatgaattt
      901 tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt
      961 atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa
     1021 gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt
     1081 cccatggaat accgcactag tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg
     1141 gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga
     1201 attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt
     1261 gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg
     1321 ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa
     1381 atggatataa atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc
     1441 ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg
     1501 cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag
     1561 atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc
     1621 cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat
     1681 tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa
     1741 gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc
     1801 tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt
     1861 gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac
     1921 catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa
     1981 caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc
     2041 gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa
     2101 agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat
     2161 cttcctttct gcacttctta atcacagaag tgtcctgaaa ccatgttaca cagctcactt
     2221 attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct
     2281 gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt
     2341 tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa
     2401 aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc
     2461 tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat
     2521 cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa
     2581 catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac
     2641 tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg
     2701 gatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga
     2761 aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat
     2821 cataggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat
     2881 ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt
     2941 ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc
     3001 attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc
     3061 cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa
     3121 acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca
     3181 aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt
     3241 atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg
     3301 cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg
     3361 gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat
     3421 tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc
     3481 aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta
     3541 tcaaattaaa aataaccatt agaaggagcg atgcagaatg gttcgggtaa agggattgaa
     3601 tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt
     3661 attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga
     3721 tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga
     3781 gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca
     3841 agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt
     3901 agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga
     3961 aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc
     4021 agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa
     4081 actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg
     4141 ggcaaatgct tttgtctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat
     4201 ttctcgtctg gttggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga
     4261 ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt
     4321 aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa
     4381 aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg
     4441 agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat
     4501 ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga
     4561 taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag
     4621 tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct
     4681 cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg
     4741 aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata
     4801 tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag
     4861 aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc
     4921 tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa
     4981 gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat
     5041 tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa
     5101 tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac
     5161 aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg
     5221 ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt
     5281 ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt
     5341 agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac
     5401 aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc
     5461 catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg
     5521 taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga
     5581 ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc
     5641 tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc
     5701 atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt
     5761 ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc
     5821 ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt
     5881 taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa
     5941 cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg
     6001 attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt
     6061 gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat
     6121 cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca
     6181 aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga
     6241 aaatgagcta aaatgaggga agataagcaa aggagcgtgg aacgatgaca aacgcaacag
     6301 aatatgttca agcaattcaa gaaaagctac atcaaaagga tcaagggcaa gaagaatttt
     6361 tacaagcaat cgatgaattc atgcccacgg ttatgtcttt tttggacact catcccgaat
     6421 atatcgaaaa gaatatctta gaacttttaa cggaaccaga aagagttatt caatttcgag
     6481 tgccttggca agatgatgaa ggaaactggc gagtaaatag agggtaccgt atccaataca
     6541 actcagctat cggcccttat aaaggtggat tgcgcttcca tcctagtgtc aatctaagta
     6601 tcttgaaatt tttggctttt gaacagattt tcaagaatag tttaaccggt ctgccaatcg
     6661 gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa atctgataat gagatcatgc
     6721 gtttttgtca aagtttcatg ctggaattgt caaaacatat cggcccatcg attgatgttc
     6781 ctgcgggaga tatcggtgta ggagctagag aaatcggcta tttgttcggc gaatataaac
     6841 gattgaaaaa atatgatact ggtgtgttga caggtaaacc tttggatttt tggggaagta
     6901 agatacgtac ggaagctact ggttatggat tagtgtatta tgtcaaacat ctattgaacg
     6961 aagaaaaaga ttcatttaat caaaaaacag tttttgtttc cggaagcggg aatgtagcca
     7021 tttatgcaat cgaaaaagtg caagagctag gcgggaaagt tgttacatgt tcagattcta
     7081 gcgggtacat ctatgatcca gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa
     7141 tcaatagaca acgtttgacg gaatacgcaa accaacgtcc ttctgctatt tatcatgctg
     7201 gagaatctgt ttggacattg aaagaaaaag cggatatcgc tttgccatgt gctactcaaa
     7261 atgaaataga cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag
     7321 aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt
     7381 ggtattgtcc aggaaaagct gctaatgctg gaggagtagc agtatctgct ttagaaatga
     7441 gtcagaacgt ccagcgattg acttgggaaa gagagcaagt ggatgagcag ctggatcaaa
     7501 tcatggaaac gatttaccat acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa
     7561 atctgcttct tggagcaaat gttgctggat ttgaaaaagt tgccaaagca atggcagcag
     7621 aaggtttagt ttgaaaaatg aaactttctt ggaaatattg tcagcatttt gtttatttgc
     7681 ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta taaggaggag
     7741 aacaaatggc acacattcat tttgactatt caaaagtagc accgtttgtc aacgaacaag
     7801 agttagggta catgcaaagc caagtaacag cagcccacaa cgaattacgg gaaggtactg
     7861 gcgcaggaag cgacttccgc ggctggatcg atttaccaac gaactatgac aaagaagaat
     7921 ttgcacgtat caaagaagca gctaaaaaaa tccaatctga ttctgaagta ttagttgtta
     7981 tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat cgacttcttg aatcacacat
     8041 tcttcaattt attagatagc aacacaagaa aagcaccaca aatcttcttt gccggaaatt
     8101 caatcagctc aacatatatt gctgacttga tcgaagtaat cggtgatcgc gacttttcag
     8161 taaacgttat ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc cgtgtattca
     8221 aagagcttct tgttaagaaa tatggacaag aagaagcaaa caaacgtatc tacgctacaa
     8281 cagataaagc aaaaggtgca gttaaagtag aagcagatgc agaaggttgg gaaactttcg
     8341 tgattcctga tgacatcggc ggacgtttct ctgtattaac tgctgttggt ttgttaccaa
     8401 tcgctgttag tggtgcagat atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag
     8461 catactcaag cgacaaacta gaagaaaacg aagcttacca atacgcagca atgcgcaaca
     8521 ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat
     8581 acttctcaga atggtggaaa caattgtatg gtgaatctga aggaaaagac caaaaaggca
     8641 tctatccttc aagcgcaaac ttctcaactg atttgcactc tttaggacaa ttcatccaag
     8701 aaggacgtcg caatattttt gaaacagttg tgaaggttga aaaacctcgt aaatcaatca
     8761 caattcctga acaagcagaa gatttagacg gtctaggcta cttgcaagga aaagaagtgg
     8821 acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc
     8881 ctaacttgct agtcaaaatt ccagaaatgg atgcttatac attgggttat acaatgtact
     8941 tctttgaaat tgctgtaggt atttcaggtt acttgaatgg aatcaaccca ttcgaccaac
     9001 caggtgtaga agcttacaag aaaaacatgt tcgcacttct aggcaaacca ggctttgaag
     9061 atcttgctaa agaattgaac gaacgcctgt aagataaggg tttttcgcta gatttaaata
     9121 atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg tacccattta ggtactcaaa
     9181 catactgcat cgtttattca gaataagggg ctgtgacaag acgtttgtca cagtcccttg
     9241 tttgtataga aatcgattga ttgccttgct ctttagtttg ttttaggact ttaagaacag
     9301 tgaggctgac ttcgaaggtt aattctgttt ttttgttttc tagaatcagt aagcaaaaaa
     9361 gcactcactt cgagtgcttt tttgcgatat attcttagct taagataggc ctaatttttc
     9421 ttttacagat gagggtacct tatcgctggt aattactttt ttctctatga tccgcttttc
     9481 gcttgctttc aactgcaaga tcgttccttc ggggtaagca gaaccattgg cagagtcacc
     9541 agaagtaatg acatcgaaat ctaactgtct tttttcaccg ttttcagcat aagcatctac
     9601 gttgtattta aaacctcttc caattacttc cccattatct gctttgatat cagtttctgc
     9661 aggcattgga gctttgacta ccgcataata atctttacct acataagtag actgatagta
     9721 gtcccaacct ttccatccta taaaagcaat caatatgata acgatcaacc ctaaaatttt
     9781 tttcatgaaa gatctccttt tttattttta tta
//
LOCUS       DBNICI010000106         9665 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.107, whole genome shotgun sequence.
ACCESSION   DBNICI010000106 DBNICI010000000
VERSION     DBNICI010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.107"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..632)
                     /locus_tag="AC1ZCC_002040"
     CDS             complement(<1..632)
                     /locus_tag="AC1ZCC_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIQ1630279.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIG"
     gene            975..1391
                     /locus_tag="AC1ZCC_002041"
     CDS             975..1391
                     /locus_tag="AC1ZCC_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIQ1630280.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1388..1858
                     /locus_tag="AC1ZCC_002042"
     CDS             1388..1858
                     /locus_tag="AC1ZCC_002042"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIQ1630281.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            1872..2645
                     /locus_tag="AC1ZCC_002043"
     CDS             1872..2645
                     /locus_tag="AC1ZCC_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HIQ1630282.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            2659..3471
                     /locus_tag="AC1ZCC_002044"
     CDS             2659..3471
                     /locus_tag="AC1ZCC_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HIQ1630283.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            3525..4490
                     /locus_tag="AC1ZCC_002045"
     CDS             3525..4490
                     /locus_tag="AC1ZCC_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIQ1630284.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            4606..7329
                     /locus_tag="AC1ZCC_002046"
     CDS             4606..7329
                     /locus_tag="AC1ZCC_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIQ1630285.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            7342..7974
                     /locus_tag="AC1ZCC_002047"
     CDS             7342..7974
                     /locus_tag="AC1ZCC_002047"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIQ1630286.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            8873..>9665
                     /locus_tag="AC1ZCC_002048"
     CDS             8873..>9665
                     /locus_tag="AC1ZCC_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1630287.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT         3260 a         1477 c         1884 g         3044 t
ORIGIN      
        1 ccaatcatat ggaagccacc gattaggtat tttatgggtt tagtcggaaa tgtttcaaga
       61 gcaatttcca tcatatcgat aatgccatga tgactacagc cagtaaagac aatcagaccg
      121 tcttcttgct caattacgac caataattca tctgaaaaat catcataaac aaatttatca
      181 tccatctttt tgtataaatt cttggtggtc gagtttgctt tgcctagggg gatgatatgg
      241 aagctcggag caagttctgt tttcgttttt attgtatgta aattctcatg cattttaagg
      301 atttcatttt taggaatacc aacatacaga aacgtggtta aaatctttaa ataatggggt
      361 aaagttacac cttcagctgt ataaataggg gcgtttcctg attttaaata tcttaacccg
      421 ccggtgtggt cagaatgacc gtgtgaaata atcactgcct ccagatcatt caaagtttcc
      481 cccattttag tcgcattata gactaatttt ttattagcgc cagcatcgaa gagatatttc
      541 ttccctgagt agttaattaa gagagataac ccatgtccac ttttaaagtt caaatttatt
      601 cttgagtttt caaataaaac cttgatttcc actataacat ctcctttcac atgaacgtgt
      661 attcatatat tgtagcacat taagttctat tttttgttgt aattaccgaa aaaaatatta
      721 atggacgctt tttacgcttt atttcgtatg tggaagaaaa tttcttagga tgtggcgggg
      781 gattggggca aagctaaatt ccaattacag tgatcattta attagacacc tttaaatacc
      841 cacactttca tcaacactat aatttaggga aagaaaagtg ttggcatgat atagaaaaac
      901 ctttaattaa cagcgtttcc taaaattggc acgcatcttg cttatagaaa agcgtaagga
      961 taggaggaat gaaaatggca actataattc ttgccagcca tggggaattt gctcaaggat
     1021 taaagcaaac agctacaatg attattggag atagtgtacc aattcatgcc ttgtcagcat
     1081 ttagagatga agatgaatct attttggtac aaataaaaaa acttttagcc acaatagata
     1141 tagaagaaac ctacatttta actgatattc taggtggtag tgtgaataat gatatgctca
     1201 ctattattaa agagtatgag ggattgcaat taattactgg aatgaactta cctttagtta
     1261 tttctattgc aacctataat ggaaaaattg gggaaagtga gctagaaaag cttattaaag
     1321 agagtcaaca atcagtgatt gattgtagaa aattattagt agaacaactt actggaggag
     1381 atgacctatg attaagttag tacgtattga ccatcgtttg ttgcacggac aggtagtatt
     1441 ttcttggagt aaatcattaa atatcaatcg tattattatt gccaataaag aggcagcgaa
     1501 tgatgaattc aaaaaaatgt ctttgaattt atctaaaccg actggcatta aattaacaat
     1561 tttttcagtt gaacagttac tagaaaaaat gcctaaaatc gaaacactta acgaaaatat
     1621 catgcttatt tttggtaata caaaagaaat tcgtcaattt tgtgaagcct atcctaaagt
     1681 acaagaaatc aactatggag ggataatcaa aaaagaaggt tccaagcaat ttagtaatgc
     1741 cattttcttg aatgaatctg agcaagaaga tgccaaaaaa ttaaaagctc ttggcattaa
     1801 acaatttatt caacaggtac caacttctaa aaaagaagat ttgaatacaa tgttataaag
     1861 gaggaatgaa aatgctcgtt cagtcaattg gtttagggtt aatcggtgtt ttttgtattt
     1921 tagactcaag gcttttagga agaatgaatt ttgaacgacc gttgattgtc agtacgcttg
     1981 ttggtttggt acttggagac atggaaaaag gattaattgt tggagcctct ttggaattga
     2041 tgtccttagg attagtaaat atcggagctg ctgcaccacc tgatatgaat atggcatcaa
     2101 ttattgctac agcctttgcc attttatcaa atgcgaatgc agaagcagca ttaacgattg
     2161 ccattccaat ttcagtgtta ggtcaaatac taggtatctt aatgcggacg attttgtcaa
     2221 atttgactca cgttgcggat cgctttatcg aacaagggaa atttagccaa gcaaggagaa
     2281 ttcatatact ttatggctcg ggattatatg ctctcatgta ttttattcct atttttctag
     2341 caatttattt tggaacgaat cttgtttctc agattgttga tgcgattcct gagtggctaa
     2401 caaatggctt aacggttgca agtaaaattt taccagcata tgggtttgcg ttgttattac
     2461 aaacgatgtt aacgaaaaaa atggtgccat ttttattact tggattttta attactgctt
     2521 attctggatt gggaatcatt ggtattgcag ccttcgcatg tattgttgcg tttgtcatgg
     2581 ttcaagttca aggtaaaaac tcaaatggta accaaaatga gccagatcca ttagatgact
     2641 tataaggagg tagattaagt gaaagagaaa gatcaaaatg catggaaata tcggcagttc
     2701 ttatggcgtt catgggcaat tcaagcctca tggaactatg aacgacaaat gaacatgggt
     2761 tttatgtacg gaattgcacc aattctagat aacatttaca agaatccaga agatgaagca
     2821 atgaagaaag aagcttacga gcggcatatg atgtactaca actgtacccc tcaaacaagt
     2881 gcatttgtgt tagggttaac tgcttctatg gaggagcaat actacgaaga ccaagaaaac
     2941 tttaatccgg aatcaatcag tgcagtgaag acttctctga tgggaccgct atctggtgtt
     3001 ggtgattctt tctttcaagg aacgattcgg gtattagcct ttggactagg aattaattta
     3061 gcccaacaag gtagtatttt aggtccaatt ctagcaatgt taatttcctt tgtaccttcc
     3121 tttttagtta cttactatgg tgggaaaatt ggctataaca tggggaataa atacttgtct
     3181 aaactgtatc aagaaggttt gatggataaa gtaatgtatg tttgctccat tgtggggttg
     3241 atggtcattg gttcgatgat tgccagcatg atcgggatta caacaccaat tcaaatgggt
     3301 aaagcttttg tcttacaaga tgtattagat agtattatgc cacaattttt accattagga
     3361 ttaaccttct tcatgtactg gttattgcag aaaaaggtca aaacaggatg gatgttggca
     3421 atctgtttag tcggaggaat tatttttagt ttgttaggta tcttacaata agggaataac
     3481 caataaattt ggttgaataa tataaataag gaggaattca attgatgaat tcaaaagaaa
     3541 ttgttaaaga aatcgtagcg aaaaaagagg taaaatcaat cgtatttgtt ggctgtggcg
     3601 cttcaaaagc ggacctttat cctgcaaagt attttctaga ccaaaatgcg aagcagttac
     3661 ggattagtca ttacacagcc agcgaattta actatgcaac accagcatct gtagatgagt
     3721 caactgtcgt tatttcagct tctttaggag gtacgactcc agaaacagtt gaagctaacg
     3781 cagtagctaa agaaaaaggt gctacagtta ttagtctaac acgaagcatt gactcaccgt
     3841 taacaaaaga tgcagattat gtaatttatc atcgatttgc agagaactat gctgctaaat
     3901 tagaaaaaac aggctactgt ttagaattgg cagtagaact tttacatcaa gtagaaggtt
     3961 atgaccatta tgaagcaatg caagaaggat ttgctactat ctatgatctt gcccaaaaaa
     4021 gtgcagaatc agctcgtaaa gatgcgaaga aatttgctga acgctttaaa gatgaaaaag
     4081 ttatttatgt aatgagtagt ggtgctacac atgaagtagc atactcaaca tcaatttgct
     4141 tgatgatgga aatgcaatgg attaattctg gtaccttcca ttcaggagaa tttttccacg
     4201 gaccattcga aattgtggat aaagacgtac cgtttatatt attaatgaat gatggaaaaa
     4261 ctagaaatat agatgctcga gcattaactt tccttgaacg ctttgatacg aaaacagaag
     4321 ttgtagatgc cttggattat ggcttgtctt cacatattgc taaagaagtc ttagactact
     4381 ttaacccatt cgtgattact gcagtattta gagtttatgc agaagaattg tcagaagcaa
     4441 gagaacatcc attaactaag agacgttata tgtggaaact aacttattaa aaaatagatc
     4501 aaactaaata aggtggcttg agaaattaag ccaccttgtt ttcttcaatg tgattttata
     4561 catatggtaa aataactagt acatattgtg aaagcaggtg gcaagatgca gcgattaaat
     4621 gactatcttt atcaactaac attgcttttg aaatctacgc cttcagataa ttattcagct
     4681 cgcagtattt cgcaagcttt acagttgaat agaagtacaa tcagtagcta cttaaatgaa
     4741 ggtgttcgtg aagggctttt cataaaagta aaaagttacc cggttttatt tttacataga
     4801 acagctcttg aagaactaca tatcacactg aataactccg aaatagaatc aattgaaagc
     4861 ttgttaacaa tttctcaaaa acccgcatta gatcaagtaa ttggctctaa aggaagtttg
     4921 aaagaagcaa ttgatcaaat taaaacggca gtcttatatc ctggaaaagg gttgccttta
     4981 ttattgatcg gcgctagtgg atcagggaaa acctttctag cgaataaaat atatgaatat
     5041 gcagtagaag aaaaggtcat tcacacatcg gcacctttca tagattataa ttgtgcacaa
     5101 tatgtgagca atccagagtt actatcctct gctttatttg gctacacgaa aggagcattt
     5161 accggagcaa gccaggagca tactggtttg ctagaaaaag ccgatggagg agtattgttt
     5221 ttagatgaag tgcatcgctt atctgaagaa gggcaagaga aattatttac atttatggat
     5281 acgggtgaat tctctcctat gggggataat agtattcgga aaaaagccga tgttcgatta
     5341 gtctttgcaa caacagaaaa tatatatacg actttcttac caacttttct taggagattg
     5401 ccagttattg ttaatctacc tagatttcaa caacgtccgt cgtttgaacg tctgtcattg
     5461 attgatgaat tttttgtttc tgagagtaaa attttagcta aggaactatc tgtttctgat
     5521 gcattgattc atttcttaat gaatagtgat tttgaaggta acgtggggaa tattaaaaat
     5581 attatcaagt atgcttgcgg aaacgcatat atccaccaga agagtcagca aacaattcaa
     5641 gttaaattat tggatatgcc tttagagtac agttataaat tcaaggagca actaaataaa
     5701 cccagaaaga aaagagcaga tcgaatctac tatccagaag aaacacgaca agtgcaacta
     5761 gaaagaaaca atcagctttt tgttcaattt tttgaccaat tacttactgg attctctgaa
     5821 gtgattgaaa ataacaagga tattaaacta tttttagaag aaatggttac caaggtaaca
     5881 caagtaatgg atacgcttat ttttcaagag gattacgaaa aagagcagtc tttatacgcc
     5941 attctaaact atcatattcg tcaaagtatt gattttatgc gagaaagcta cgggtttgaa
     6001 caagatggta accgggtagt cgctttggcc aactatctat atgtcaagga taatcaagtg
     6061 atttttgaaa atagtcttca agaaaagaaa accaaagatc agcttaacga attttttgct
     6121 aatgttcttg aaacccctta ttggtatgca aaaaaaatct taagcctttt atctgagtgt
     6181 ttagatcaac ctgtgtatga agaggacatt ttctttatta cgctatattt ttatagtttg
     6241 aatgtcacga atgttaaaac aggtattaag agtattgtgc tagctcatgg ttattctaca
     6301 gctagtagta tggcgaatgt tgccaaccgt atgctgaata agaacgtttt tcaagcttat
     6361 gatatgcctt tagatattac attagagaaa attgaagcaa aattgattca attccttgga
     6421 gattatcgaa cagattcagg cttgatttta ttggtagata tgggttcact aaaccaacta
     6481 ggcggtttat tagtgaatca tctaaatgga ccactgttaa tcattgatca tatcaataca
     6541 ccgttattat tggaagtcgg ccaacaagta ttaaacaata aaagtatcac cgaaatagga
     6601 gcagaaattg aaacaagtct tactttcaaa aagcagttac tacttcccaa gaaaaagaaa
     6661 aagaaagcca tcattacttg ttgttataca gggattggta gtgcgaccca gattcaggag
     6721 attttaacga aatgcttgaa agatgcagtt agagaattag taattattcc gtatgattac
     6781 aaaaagctgt tagaaaacaa gaattttgag actccttttc aattatacga tgtcatcatg
     6841 attgtaggta cagaagatcc caaaattaat cagattccat atattggatt agatcgcctg
     6901 attaatgggg aagcaattgg cgaatttgtt cattacttgg aaaaagagct gactattgaa
     6961 gtgaacgatt tacaaaaaga attaattttc aatttttcaa ttagtaaaat tgtcgaaaat
     7021 ttaaccattt tggatccaga taaggtgcta tcttttgtac aaaaagcaat taaagaatta
     7081 tcgaagttat taaattttga tttttctaat aatcagctgt ttttgttgta ccttcactgt
     7141 agttgtatga ttgaacgaat tgtacgtaaa gaaagtgtgg atgagcaaga ggacattgaa
     7201 gaatatatgc aagcaaatgg agaaataatg gataaaattc atagcgcttt ttttgagatt
     7261 gaaagggaat acacaattga aataccagat ttagagttgc gattattgag cgatattgtt
     7321 caaggatagg agtgaattcg aatgacaatg ggagtaattt ttgatatgga tggggtactc
     7381 atagacagcg agacatttta ttttgagaga agaatgaact tctttcaaga actggagata
     7441 gatccaggat caaaccagat tgatgattat gtggggaaaa cagaagctgc aatatgggaa
     7501 acattagttc ctgaggacaa atcattaaga gaggaacttt atccgaaata tgtagattat
     7561 cgaaaaacgc atccaattga tttcgctaaa gatttacgaa aagaagtgaa accgttactt
     7621 gacttcctca agaagcggga tataccattg gcactggcat cttcttctcc agttgttgag
     7681 atagagagta tgctcattca atgtgaactt actgaatatt tttcctttgt tataagtggt
     7741 gagtcattaa aacaaagcaa accacatccc gaaatatatt taaaagcgaa acagcaatta
     7801 gactgtgact tctttattgc agtagaggat tcaacgattg gtattaaatc agcaaaggca
     7861 gcaaatatct atacggtggc actaaaacaa ccaatgcaaa tcgatcagag tgaagcagac
     7921 atacaaattg aaagtctaca agaactggaa caaattgttt tagaaaaagg ttagtgaatt
     7981 aatggaaata aggctatata tctgtgctag ctattttgac taaccaaaaa aacttgctat
     8041 aattcaggtg tagtaaaagg tgctggtaac accttttacg aaaatgcttc ggcagtgata
     8101 atgggattgg ctttttaagt tttttatttt gttcgcaatc cctaaggtca tgtgaatgat
     8161 cctagggatt gctagaatat tttgcaacat tctatctatt tcagaacgta accttcccca
     8221 gttcattggg attcattagc atacaaccaa catttcaaaa agaaattgca ctacaccatt
     8281 attagctatt ttgactaacc ccaaaaaact tgctataatt caggtgtagg taaaaggtgc
     8341 tggtatcacc ttttacgaaa ctgcttcggc agtggtaatg agtgtggctt aaaggactag
     8401 tcctttaagc tttttacttt gttcgcaatc tctaagatca agttaatgat cgataagatt
     8461 gctagaatat tttgcaacat tctatttcag aacgtaacct ttcccattcc actgtgattc
     8521 attggcaacc ctcctcacaa ccaacgtttc aaaagaaatt gtaaagttgt tggggtacca
     8581 cgaccgaaca atactacacc tgaatgatag taagttgtct cattaagtat agcatatcct
     8641 ctttttgggt aaatagaaat cggttacatt ttttatccaa gaccaagttt tttcatttgg
     8701 tcttatttgc tatacccaaa caattccgca gtatagtcga cggtattgat tttatcaggt
     8761 gagtaataga aacttgtttt ccttccccaa aacattacct ataaaggaga aaaaatggta
     8821 tcgtaattat ataggtatag gatgaaattt ttttctaaga ggagggttgt tgatgaaaaa
     8881 tttacagtta aaatttattg ttgatccatc gattgtacgc tggtttcgga ttatcaatca
     8941 atttgagcgc aatcaacttg ccacaaaagg agaattagca aagagtaatc atgtatctga
     9001 acgaaccatt caaacagatg tcaataaaat gaaggactac ttttctgaaa gtgttcaatt
     9061 tgtttcaacg aaaagtgggt attcttttca gaaggaaaga ccggttcttt ttctcaagca
     9121 gaaggagcaa ctactggaga acgaaatttt atttgagttg ttagggaatg tcttttatgg
     9181 cgaattggaa gatatgacgg agctaattga tcgttttcat ttatctgaag cgacttttcg
     9241 tcggtatttg aagcagattc aaccagtttt ggaagaatac ggcttagaat tgagtcttta
     9301 tcctcttgat ttaaaaggag aagaggcaaa tattcgcaag ttttttaaag acttctatta
     9361 tgaaggggag atgacgcccc atactcttgt tccaccaagg gatcttcatg aacttgtgca
     9421 agctacattc tatgataaat ttgagtattt ctcgattgga acagggactt ctcctgctga
     9481 attttattat agtttgtata ttgcgattga gcgtgtgaga caaggaaaaa cgatagccat
     9541 tcctcaaggt ctgattgagc gagtaattgc tagtgagaat tttcgcttaa tgtattcctt
     9601 gaaagaagcg attcaaacaa attatcaagt tgctttatca gaagaggagt tttgttggtt
     9661 gtatt
//
LOCUS       DBNICI010000107         9525 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.108, whole genome shotgun sequence.
ACCESSION   DBNICI010000107 DBNICI010000000
VERSION     DBNICI010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.108"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(81..1532)
                     /gene="cls"
                     /locus_tag="AC1ZCC_002049"
     CDS             complement(81..1532)
                     /gene="cls"
                     /locus_tag="AC1ZCC_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HIQ1630288.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1873..4539
                     /locus_tag="AC1ZCC_002050"
     CDS             1873..4539
                     /locus_tag="AC1ZCC_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIQ1630289.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4903..5655
                     /locus_tag="AC1ZCC_002051"
     CDS             4903..5655
                     /locus_tag="AC1ZCC_002051"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIQ1630290.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5677..6831)
                     /locus_tag="AC1ZCC_002052"
     CDS             complement(5677..6831)
                     /locus_tag="AC1ZCC_002052"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HIQ1630291.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7016..9457
                     /locus_tag="AC1ZCC_002053"
     CDS             7016..9457
                     /locus_tag="AC1ZCC_002053"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HIQ1630292.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         3077 a         1712 c         2033 g         2703 t
ORIGIN      
        1 ttcggaagaa gggactgtga caagattttt gtcacagtcc ctttaatctt agtatatttt
       61 tctctacttg aataaaaaaa ttataggatt ggagaaaata atcgagaaat ttgttgcttg
      121 aataatagcc agttagacat ttccttgact gtttcagttg tcatggatga acaatgtgtc
      181 tcatcttctt tgaactgagt agcaagttct tctaaaaact caggatcgta aatgacagca
      241 cttgtttcaa aattcaatcg gtagctacga atatcttgat ttgctgaacc taccatgcag
      301 atttcatcat ccattatcaa tgtttttgca tggaggaagc cgttctcata gacaaggatt
      361 tgtacatttt ccttgatcag ctgccgagca taatattgtg ttgctcgata aataaaagga
      421 tgatccggtt tgttgggaat catgattttc acatctacac cagaggcagt tgcgattttt
      481 aaagcagcaa tgacactttc atcaggaaca aggtaaggtg tctgtatcca aacacgtttt
      541 ttagcagaag taatcaattt gataaatgaa agcttgattt gttccctttc gttattcggt
      601 ccgcttgaaa caagctggat ggatgtatca gcaagcttag attcatctcg atctgctttt
      661 ttaaagaaat aattcaaatg atagccgacc tttttttctt caggtaccga gacgttccag
      721 tctgttaaga agcgaagctg gagcaaagaa gaagcggcac cgaaaatccg tatatgcgta
      781 tcgcgccaat agccgaattt ttttgttgta tttacatatt gattggcaat attgaaacca
      841 ccggtataac taatctttcc atcgatcaca acgatttttc ggtgatcatg gtaattcaat
      901 cggaaacgaa gtaatgccct ttgtgaggta acaaatgtat ggacatgtcc accatttttg
      961 attagacgat tgaaatcttt tgcttttgtg ccatgagagc caaatgcatc atataatatt
     1021 cgaacttcca cgccttctgc ggctttttct tctaaaacat gtaagacttg ctggccgata
     1081 ttatctgtca caaatgcgta atattcgata tggatcgaat gtttggcttt ttttagatct
     1141 tgaagcagtg aatccaattt ctcttgtccg tctgtgagaa gagtgacaga attcattctt
     1201 gtcagcggca tacgatttaa tgaagagaag aagtcaacaa attgctgttt gtctttatcg
     1261 cccatgtcag gatcgtaatg ttcaatggta tctcctctaa aagactgaaa gttttctaat
     1321 tctttcaagt cactttgtcg gagatagaat tttttcttat ccgttaatcc acgtccgaaa
     1381 aataaatata atacaaagcc caccccagga agggcaaaca atactaagag ccatgcccaa
     1441 atggctgcta catctcgggg tttgatcaaa atagccacca aagcaataag tgcatttaat
     1501 agataaaggg cggttataat actagatacc acaaaaatgt tcctccttac atacaataac
     1561 attagtatag catgatagaa gaaagaagcc taccaaggtg aatatacttt tcactccttt
     1621 catgaaaatg taggtaaatc gggctaaagt agttgctttt tgtaggttaa taaggtagga
     1681 tattagtaca gttgaatatc tccttagggg agtaaccggc aacgtttgtt gcgataatat
     1741 caacagtaag ttaaccaaat ggttttctgg tattgtatga aatggtgaga cctaagacaa
     1801 atgcgtattt gtcttaggtc tctttttttt cagacaggac tagggccaag atcaaaaaat
     1861 gggggatgaa atttggaaaa caaggaacaa gcacaacaag cggtctataa agaaacgatc
     1921 gactcgttga tgcagcaaac gcagagctca accgatggat tgagcaccac gcaagcgaag
     1981 gaaaaattag aaagagatgg cttgaaccag ctaaaagaaa agccgaagaa gccaacttgg
     2041 aaattgttct tagaaacttt caaagatgca atggttatcg tattgctgat tgtcgcactg
     2101 attcagatgg tcatgggtga ctacatcgaa ttcttagtta tttttgcagt attgatgttg
     2161 aattctataa tcagtgttgt ccaaacaaaa aaagcagaag gttctttaga agcgttgaaa
     2221 aacctttcag cgcccgatgc tcgagtgatt cgtgatggta aggaaaaaac gattccggca
     2281 aacgaacttg tggtaggaga tatcgtgttg ttagacgcag gcgattatgt acctgctgat
     2341 ggacgtttac tagatgcggg atcattaaaa gtagatgaag gtatgctgac aggagagtcg
     2401 acttctgcag aaaaagaagt aacggatatc acaagtacgg taccaatcgg agatcgtaca
     2461 aatatggtat tcagtggaac gatcgtaact tacggacgcg gtaaattctt agtgacttca
     2521 actgggaata atacggagat cggacaagtt gctggccttt tggaatcaac aactgagcaa
     2581 gtcactccgt tacaacgagg tctggataaa ttcagtaaaa aactaagttt ggctattttg
     2641 gcattgtctt tattgatttt aggtatccaa ctgtttcgta tttatcttgg agaaggtaca
     2701 ggagatatga ctgcagatat cgtcagcgca gtgatgtttg ccgtagccgt agccgtagct
     2761 gctattccag aagcattaca gtctatcgta acaatcgtgt tatctttggg aacgaataaa
     2821 atggcaaaac gtcatgccat tattcggaag cttccagcag ttgaaacact tggttcgaca
     2881 agtatcatct gtacagataa gactggtaca ttgacacaaa acaaaatgac agttgttgac
     2941 tactttttag gtaatggcga tactggggat ttcacaaatg acccttcaaa atggacagct
     3001 gacgaacgtc gcttgatcga aatcagtgtt ttagcgaatg atgcatcgat cagtgaagac
     3061 ggtactaaat tgggcgaccc tacggaagta gcgtttatcg actttagtga aaaattaaac
     3121 cagccttatc aggaaatccg aaacaactac ccaagacaag cagaacttcc ttttgactca
     3181 gatcgaaaat taatgtctac tggacacaca attgataaca aaaattactt gtttgttaaa
     3241 ggcggaccag atatcgtctt ccaacgcagt aaaaaagtat tgctaaatgg tgaagtcgtt
     3301 ccgatgacgg atgaattgat gaaaaaattc caagagcaaa atgaagcttt ttcaacacgc
     3361 gcattacgtg tactagcttt tgcttaccgt ccacttgata attttgattt atcatttgaa
     3421 gatgaagacg atttgatcct tgttggtttg cttgctatga tcgatcctcc acgtgctgaa
     3481 gtgactcagg cggtaacaga agcaagaagt gcaggaatca aaacaatcat gattactggt
     3541 gaccacaaaa caacagctgt tgctatcgca aaacaaatcg ggatttttga agaaggaaac
     3601 ctggctttga ccgggacgga actagatgaa ttgacagaag atcaactaaa cgaagtttta
     3661 gaaaaagtca ctgtttatgc acgtgtttca cctgagaata aaattcgtat cgttcgtgct
     3721 tggcaaaata aaggtcatat ctcagctatg acaggtgatg gtgtaaatga cgcaccagca
     3781 ttgaaacaag cagacatcgg aattgctatg ggaacgggta cagatgtggc aaaagatgca
     3841 gcagcgatgg tattgacgga tgataacttt gcttctattg tcagcgcagt agaagtagga
     3901 cgtaatgtgt ttgataatat caagaaagct atttcttatc tatttgcagg aaatttaggt
     3961 gcgatcattg cgatcatctt tgccttagtt gctgactggt ccaatccatt tacagcatta
     4021 caactgttgt tcatcaattt agtcaatgat tcactgccag caattgcatt aggtacagaa
     4081 aaagctgaac ctgctacgat gaaccgcccg ccaagagatc caaattcagg aatctttacg
     4141 gacaaaacat tgatttctgt aacgtatcgt ggtgtgctga ttgggattgc tgttatcttt
     4201 gctcaatggc ttggcatgca gcattcacct gaattcggtg tagccatgtc attttcaaca
     4261 ttgatttggt gtcggacatt gcaaacattc ccagcacgtt caaacacaca aacagctatc
     4321 caagcagggt tcttctctaa cagaagtgtc ttactagctg tagttgtatg tagcgcgctt
     4381 tactgcttaa caatgatccc aggactgaaa gaagtcttct ctatcccatc agcatttgga
     4441 ttgacagaag tctttacatg tatgggaatc gcacttgtga caattgtttt gatggaaatc
     4501 actaaattga tcattgtacg agtacaagga aataaataaa acgaatcaac gaggttgtga
     4561 gcctgccgtt taattctgag tcacaaggtg caatttcaac aaacagcttc tcatgtttgt
     4621 tgaatattgt gacttgtgac cgaagagttg gcaggagaac accgtttaag gaagaagttg
     4681 taaaatcagc attttgagct aatactgagg gtcatctgat tggacactgt ttattcggaa
     4741 agagggactt tgacacgact ttggtcacag tccctttttt tatttatgat agacggaaag
     4801 ccaatcattt tattattcat caaaagagat agaatagata aaagaggaga atgatttatt
     4861 ccgaaaagtg aaaacatata acaaaaaatt agaggaaatc aaatgaatga aaaagaatgg
     4921 gatgaatttg ctgaagaata ttatgaaatc cagcaagaat cgcaagtaac tattgctgaa
     4981 gatgtaaaaa attatctgaa aacggagaaa gtcctgcctg ctgaaacagc agcagacgtt
     5041 gctggaggat ctggcagatt tctttcctta gcaaaagaaa ttaatcgcta tgatttgatc
     5101 gatttttctt caggaatgct agaaaatgca aaaaaagaag cagaaaaaaa tgagttgcca
     5161 aatgtttcct ttatcaaaca gtcgttagaa gaatttatcc aatcaaatga gacatatgaa
     5221 atggttttta cagcagcaaa tccagcattg gtacatctta ttcaattaga aaaattgatg
     5281 aaaaagtcaa gcaaatggtg cgtgataatc agagtagtgg aatcaatcga tgatgttttt
     5341 tatcctatcg aaaagcaact aggaatgata gaagaagatc ctaatacatc gccagttgta
     5401 atggatcaat ttgaaacata cttaattgat gaaggattca aagtgaagaa aaaagatttt
     5461 acttatttta cacaggaatc aattactgaa gaattgatga agctatacta tgacgcgtac
     5521 gcaaaagatc ctgttgtgac caggtacatt cgcaaactat tcagtgaaaa acgagaaatc
     5581 cttagtacga ctaagctgac ctatagactg ctcctttggt ccgcagataa aaaaagaacc
     5641 tttcacgact cgtgaagggt tctttttatg attttattat attttgtagt aacgaatatc
     5701 attttcgatc tttgtaatcg aaaaaggtac atctgaagta tattctgcac attctccgtg
     5761 ttcaggaagc gtctcactga aaatcgtttc atattcgtca acagtgatcc gtgttcgaga
     5821 atctagcatc tcttgatgag attgtgcgaa gagatattct tgatagtttt cttccagata
     5881 acctgtaaaa aattcactta ctgcaccaga accgtagctg aatagaccga tgcgatctcc
     5941 tggctgtagt gatttcgaat tttccagtag agaagttagc cccaggtata atgaaccagt
     6001 gtaaagatta ccgattcgtc ggctgtaacg gatgctttct tcatagcgag ccataagacg
     6061 ttcctgattt tcttcgtctg tttggtctaa tacgctttgc aatgcctttt ttcccatctt
     6121 agtatacgga atatggaaag caatcgcctg ataatcttcg agtcctcgac ccgacaattc
     6181 tttatgtcga ttccaaactt tttggaatga ttcgatatac gtagaattag ataaaggacc
     6241 atcaacaaca ggaaattcgc tataatctgg acgccagaaa tcatagatat cttctgtcag
     6301 aaatacgctg tcgtcttcaa tcgataaaat acgcgggttt tgagtgatca tcatcgcaac
     6361 agcaccgaca ccttgcgtca cttcaccacc gcttgccaag ctgtaacgag caatatcact
     6421 tgctatgact agtacttttc gttctggatg atttttgaca tattctttcg ccatgtgcaa
     6481 agctgcggtg ccaccgtagc aagcctcttt tacttcaaaa gaacgagcaa aagattggat
     6541 tttcagtaaa tggtggataa ttaccgcact cgcttttgaa tgatcaatcc cggattctgt
     6601 tccgacgatg accatgtcga ttagctcacg gtctttttct gttacgattt tgctggcagc
     6661 atttgctcct agtgtaatga tgtcttcatt tgcacgattg actgccattt ggtcttgacc
     6721 aatccctata tggtattttg caggatcatc cccacggctt tctgccaact ctgtcatatc
     6781 taaatataaa ttaggaataa aaaaggaaag acgatcaatc cctattttca tttcaacacc
     6841 tctttactta tgataaaaaa cttactttac taaggtatca taaaaagatt aaaagaaata
     6901 taaaaaagag aatatttaag gattgttaaa atttaatctt agaaaaaaat aactggaaca
     6961 agagctattt gtttttacag caaagaatgg tatatttttg taggaggtgg cttacttgaa
     7021 agaagtcgtt atgatagatg ctgcaagaac gccgatagga aagtatcgcg gaagtttaag
     7081 tccatttact gcagttgaat taggcacact tgtgactaaa ggattattag ataaaacaaa
     7141 actgaaaaaa gataaaatcg atcaagtaat atttggaaat gtcctgcaag ctggtaatgg
     7201 tcaaaatgtt gccagacaga ttgctttgaa cagcggtctc cctgtagatg tcccagcaat
     7261 gacgatcaat gaagtttgcg gatctggtat gaaagcagtg atcttagcac gccagctgat
     7321 ccaattaggt gaagcagaac ttgtgatagc cggcggaaca gagagtatgt ctcaagcacc
     7381 tatgctgaaa cagtatcagt cagaaacaaa tgaatatggt gaaccaattt ccagtatggt
     7441 caacgacgga ttgactgacg cattttcaaa tgcacatatg ggattaaccg cagagaatgt
     7501 tgcaacacaa ttttctgtga gcagagaaga gcaggatcgc tatgccttgt cgtcccagtt
     7561 gaaagcagca catgctgtcg aagccggtgt attttctgag gagatcatcc cagtcaagat
     7621 ttctgatgaa gacgtgttat ctgaggatga agcagttcgt ggaaatagta cattggaaaa
     7681 actgggcacg ttacgtacag tattctcaga agaaggaact gtaacagcag gaaatgcttc
     7741 cccgttgaat gacggtgcct ctgtggtgat ccttgcatcc aaagaatacg cagaaaataa
     7801 taatctgcct tatttagcaa ccatcaaaga agtagcggag gtcggtattg acccaagtat
     7861 catgggaatc gctccaatca aagccattca aaaattgaca gatagatcag gaatgaattt
     7921 atcaacaatc gatctttttg agatcaatga agcatttgca gcttcttcta tcgtagttag
     7981 ccaagaactg cagttagatg aagaaaaggt caatatatat ggaggagcaa tcgcattagg
     8041 ccatccaatt ggtgcaagcg gtgcacgtat cttgactaca ttagcttacg gattgcttcg
     8101 tgaacagaag agatacggta ttgcttcatt atgtattggt ggaggtttag gcttggcagt
     8161 cttgttagaa gcgaatatgg agcaaacaca taaggatgtg caaaaaaaaa agttttatca
     8221 gcttacgcca tctgaacggc gcagtcaatt aatagaaaaa aatgtattga cacaagaaac
     8281 tgcgttgatt tttcaggaac aaacactttc tgaagagctc tccgatcaca tgatcgaaaa
     8341 ccaagtgagt gaagtggaaa tcccaatggg gatcgcgcag aattttcaaa tcaatggcaa
     8401 gaaaaaatgg attccgatgg caacagagga accatctgtc attgcagctg caagtaatgg
     8461 tgcaaaaatc tgtggaaata tctgcgcaga aacacctcag cggctgatgc gagggcaaat
     8521 cgttctatca ggaaaatcgg agtatcaagc agtgatcaat gcagttaatc atcgaaaaga
     8581 agagctgatt ctttgtgcaa atgaaagtta tccttcaatc gtgaaacgcg ggggcggtgt
     8641 tcaagatatc tctactcggg aattcatggg gagtttccat gcatacttat ctattgattt
     8701 tttagtggat gtaaaagatg caatgggcgc taacatgatc aactcgattc ttgagagtgt
     8761 ggcaaacaag ttacgggagt ggttccctga agaagagatt ctattcagta ttttaagtaa
     8821 ttttgctaca gaatcattgg cttctgcctg ttgtgaaatc ccattcgaac gcttagggag
     8881 aaacaaagaa atcggtgaac aaattgccaa aaaaatccaa caggcagggg aatatgcgaa
     8941 gcttgaccct taccgtgcag caacgcacaa caaagggatc atgaatggta tagaagctgt
     9001 agtggcagct acaggaaatg atacacgtgc ggttagcgct tcgatccacg cctacgcggc
     9061 aagaaatgga ttataccaag gattaacaga ctggcagatc aaaggggata aactagttgg
     9121 aaaactaacg gtgcctttgg ctgttgccac agtaggcgga gcttccaata tcctgccaaa
     9181 agcaaaagca tctttagcta tgttagatat tgattcagcc aaggaactag cacaagtaat
     9241 tgctgcagta gggttagctc aaaacctggc tgctttgcgt gcattagtta cagaaggaat
     9301 ccaaaaaggc cacatgggac ttcaagctcg ttcattagcg atctcaattg gggctattgg
     9361 tgaggaaatc gagcaagttg cgaaaaaatt acgtgaagca gaaaagatga accagcagac
     9421 agctatacaa atattggaaa aaatccggga aaaataaaaa agcaatatct ttagaagaat
     9481 aaagcaagat ttacagttat tttatttact gcagatcttg ctttt
//
LOCUS       DBNICI010000108         9489 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.109, whole genome shotgun sequence.
ACCESSION   DBNICI010000108 DBNICI010000000
VERSION     DBNICI010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.109"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            629..3856
                     /gene="fss3"
                     /locus_tag="AC1ZCC_002054"
     CDS             629..3856
                     /gene="fss3"
                     /locus_tag="AC1ZCC_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HIQ1630293.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYCQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3969..4283
                     /locus_tag="AC1ZCC_002055"
     CDS             3969..4283
                     /locus_tag="AC1ZCC_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HIQ1630294.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            4296..4670
                     /locus_tag="AC1ZCC_002056"
     CDS             4296..4670
                     /locus_tag="AC1ZCC_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HIQ1630295.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4680..5015
                     /locus_tag="AC1ZCC_002057"
     CDS             4680..5015
                     /locus_tag="AC1ZCC_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HIQ1630296.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            5098..6438
                     /locus_tag="AC1ZCC_002058"
     CDS             5098..6438
                     /locus_tag="AC1ZCC_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HIQ1630297.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            6516..7190
                     /locus_tag="AC1ZCC_002059"
     CDS             6516..7190
                     /locus_tag="AC1ZCC_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HIQ1630298.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            7183..7347
                     /locus_tag="AC1ZCC_002060"
     CDS             7183..7347
                     /locus_tag="AC1ZCC_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630299.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            7423..8565
                     /gene="dcm"
                     /locus_tag="AC1ZCC_002061"
                     /pseudo
     CDS             7423..8565
                     /gene="dcm"
                     /locus_tag="AC1ZCC_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            8555..8845
                     /locus_tag="AC1ZCC_002062"
     CDS             8555..8845
                     /locus_tag="AC1ZCC_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630300.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            9104..>9489
                     /locus_tag="AC1ZCC_002063"
     CDS             9104..>9489
                     /locus_tag="AC1ZCC_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1630301.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGV"
BASE COUNT         3145 a         1609 c         2089 g         2646 t
ORIGIN      
        1 caaacatgag aagcaatttt tgtaaattcc ttcttatttc ttgaagctga ccacttctgt
       61 cacaacctca gaagcggata aataatattg tttgatgata ctttatcttt ttcccgtttg
      121 tttatagatc gaatgatatt atagaccaag aatcctacct attaacatag ctaaaaacac
      181 aaaaaaggca ctttccaaat tgtggaaagt gccagaaaag ttaggattaa ggtaactgcg
      241 ctcagcattg cttcgcccag tacgattctc gtaactcgtg aactcgaacg ataatcgcaa
      301 ttaacgtttt gagaactgtg aagctttacg ggctttctta agacctggtt ttttcttcgt
      361 atcattgaat aagtttgaaa aaccttgatt tataaaggtt ttcccgtaaa tatattttat
      421 tatatttgat taaatttgaa atgtttgtgt tctaaaatgt gttctaattt aaaggcagta
      481 tatttcgtat tttagattaa atttttaggc tcattcattc tcaaatccat tctattcagt
      541 caataaaaat tttaaaaaga ctttataaac aaagatgttc aagcgagcat cttatttttt
      601 tgtgcttaat ttgaaaggag aacatttcat gtataaaaaa caaaaaaaga aattatgtgt
      661 attaactatt ttgagtttgg ctgttcaagc aattgttgga gcaattgctc ctacgattac
      721 ttttgctgat gaaattaccc atccacaaac ggtaaccgta gaactagatt tagcacatca
      781 atatgctgtt gaaggaactt ttagtgatgg tcgtccaatg tcagaagtca ctgttccgca
      841 ttatgcagtg tacaacggct ttaaacaaga tattttctgt attgaaccag gagtcccgat
      901 ttacaatgag ttcactcctg gctatgagaa aaacccgtta ccagatatgt ccgataaagc
      961 caaattagtt tccgttttat ggaaaaaagt aggtacagat gcagatactc atattgtggc
     1021 acagaagatg atctggcaag aagttaatgg gtataccctt cactcaataa aacgatcaga
     1081 tggtagtgcc gtaaatatcg cagccattga ggcaaaaata aatcaagcca ttgacgatta
     1141 tcagaagaaa ccaaccttcc ataatagtac ggctaaaaca gtattagggc aatcgactac
     1201 tgtgacggat acgaataatc tgaatttgtc agagttcgat gaggttgtgg aaaatgccgc
     1261 aaacattgat tatcgtgtga atggtaatca gttgataatc accccaaatg ccaattcaaa
     1321 agaaagtggt gtgctgacgc ttaagaaatc tgctggtacc ggaacacctg tcgcctataa
     1381 aaaggctggc caacagactt taatggccgg ggcgattgat aaacctaata cctacacaat
     1441 caaaattgat gtggaaacgg agggttcttt aaagatcaaa aaagtcgata aagaatcggg
     1501 tgatgttgta ccaggaacgg ttttccattt agactttgga aaaactttac ctgcaaaaga
     1561 cgtgactact gacaaagaag gcattgctac attggatgag attcctcatg gtacaaaagt
     1621 aaccattacc gaaaaatcag taccggcgcc ttatacgatt gataccaccc ccatgactac
     1681 caccattaaa gcaggcgaaa ccatttacgt gacttcaaaa aacgcacggg aaaaagggca
     1741 aatcattctg gataaaagcg gagttgaaac agggagtgat ctttggaatg acaattattc
     1801 cttagctggt aatacgtttg ccattcgcaa agacagtcca accggtgaaa tcgttcaaga
     1861 aatgaccacg gatgaaaacg gtcatgcgga aacaccaaaa gagattgcta atgcgttgga
     1921 attgggaacc tattatgtga ccgaaactaa agctagtcat gggttcgtga atacgttcaa
     1981 acctgtaaaa gtcgaattga agtatgccaa tcaaaccgta gcccctgtta ccagtaacgt
     2041 aaaagggcaa aatcaagaag tcactggcga aaccactttg acgaaagaag acaaagatac
     2101 tggggataaa gcacaaggta aggcagtctt tgagggtgct gaatacacct tgtttactgc
     2161 taaggatgga aaagctgtta aatggagtga ggcttttaaa ccagagttag cgaagggaac
     2221 gaaaggttct gatgaaacgg tcactttgac tttagacgaa aagaaccaag ttgccgttaa
     2281 acatttagct atcaacgagt attattgcca agaaacaaaa gcgcccgaag gatatagcct
     2341 tgatgaaaca aagtatcctg tatccatcaa aaaggtggat gatagcgaaa aaaatgccgt
     2401 gattacccga aatgttacgg caaaagaaca agtgattcgc tttggctttg atttctttaa
     2461 atttgctgga tcagctgcgg gcactaccga aactggcttc aatgatctga cctttaaagt
     2521 gtcgccattg gaagggacca atgaaatcac aggtgccgaa gatgaagcga ccacagctta
     2581 taacgagcaa ttagggtttg atggttatgg caagtttgaa aatcttcctt atggggatta
     2641 tttacttgaa gaagtagaag ccccagaagg ttttcaaaag attacgccat tagaaatccg
     2701 ttctactttt aaggaaaaca aagaagactt tgtgaagagt gagtatgtct ttaccattac
     2761 cgaacaagac caaaaacaac caattaaaac ggtgaccgtt ccttacgaga agctgacgaa
     2821 caaagcattt tctgttagtt tgaaccgttt aatgctctat gatttgcctg aggaagaaga
     2881 tagtttaact tctcttgcga cttggaaaga cggaaacaaa gaattaactt ctctggattc
     2941 taccgagcta gttgataaat tgagttacaa tctacatgaa atcaaagaag actggtatgt
     3001 ggtagcgcaa gccattgatg tggatgcgac aaaagctgcc caagaaaaag atgaaaaagc
     3061 caaaccagtg gtgattgcag aaacaagcgc aacgttggcg aacaaagaga aaacaggaac
     3121 ttggaaaatt cagcataaat taaccgccga acaagtttta aataaaacta ttgtcttatt
     3181 caactatgtg tatgaaaata aggaagcttt tgaagcaggc gataagccag tagcgaagga
     3241 tgttagcttg aataaccaag cccaaactgt cagttgtaca gtggaacacc atgtttctat
     3301 ccaaacaaag gcccacctag aaaacggttc tcagaccttt actcatggtg atgtggtgga
     3361 tatgtttgat gatgtgtcga ttactcatga tgtactggat ggctcaaaag aagctttcga
     3421 aacaattctg tatgctttat taccggatgg tacaaacaaa gaaatttgga aatctggcaa
     3481 gattgattat gaagtaaatg ataaagaatt caccaaaact gtgcttgcga aaaaagtaga
     3541 taccggaaaa tatccagaag gaaccaagtt tacttttgcg gaaatcaatt acgataaaga
     3601 tgggaatatc aatggaaaac acaatgaaga tttgaaagaa aaatctcaaa ccttaacacc
     3661 aaaagaagtt ccaaccatac taagtacgcc gaaacaaccg gaaacaccaa ctgttccaag
     3721 tggctctcaa gaatctagtc ccacagtgaa gaccttccct caaaccgggg agaaaaattc
     3781 caatgtttta ctgttcattg gttttacttt gatttttgcg acggcgggct attatttctg
     3841 gaatcgccgg aactaaggtg atgagatgat acaagtaaat cctaagaacc aacgtgcgcg
     3901 accgcctcct aaaaaagtag tcgcgctatt tttgtatcca aaactaacaa tgaaaagagg
     3961 aaaagaagat ggaattgaaa tttgtcgtgc cagatatggc tgaaaccttt ggaaagttaa
     4021 gttatgccgg tgaaggagaa attataacag aaggatatgg acggaatact acggtgattg
     4081 gtcgcagtta tcatttgtat tccagtaagc aacgggccga tgatattgag gtggtagtag
     4141 ctgcggaagc cggtgaaaag gattttgatc aagaccaacc gctaaaagcc gtgaatcccc
     4201 atttggttgc caaaggctat gagattgaga atcgtgggtt tactgattac gtgttgtatg
     4261 ttgatgattt agtgaaagca taggaggaaa agaagatgag attaacagaa ggtattgtcg
     4321 tagattcagg tttaacattt gggaagttgc gtttttccgc attacgccga gaagtacgga
     4381 agcaaaatga ggatggaaca attggtaatg aggtaaaaga acgaacctat aatttaaaat
     4441 cctctgcaca agggcgaatg attcaagtta gtatcccagc taatgtaccc ttacgcgagt
     4501 ttgcttatga tgcagaggta gagttggtca atccaatcgt ggataccgtt gccaattatg
     4561 tttttcgaga aggaactacc gttaattggt tcattaaggc ggatgattta gtcttgaagc
     4621 ggcagccgaa tcaaggaaat cctacgaacc aaaatgaaag aaagaaatag gtgaagatta
     4681 tggaaattaa tgttgaacat attttagatt gtctcgatca atatggaaaa ggcgagctga
     4741 ctgaggaaca actcgttaaa gcattaacct ctgatgaaaa aatgtttctg attatacatc
     4801 aaggtttact tggagaaaat agcgacagtg aagaagactc tgatgtacta gactggttag
     4861 gagaagaaga gtctttcttt atagttgtcg aagtaaatga aaacttatgt cggcaggcag
     4921 aatccatttt ggaagaaatt ggtgtggaga tgccggatgc tattgaaggc tttttaaatc
     4981 aattggttga aacgaaacaa ctcccctcct cgtagcagta aatgactagc ataacttttt
     5041 gaaattgcct aatgaaatcc tattgggctt tttcgattgg aggtggaaac catcaaaatg
     5101 tacaaaggac atcgcatacg cgcaggagat cagcacttgg tttatcactt tgttcttggc
     5161 tggctactcg ctctgttcat cggttggatg agcgtctttt attttcaaga gtttagacaa
     5221 tttgatattt ctaagctatc actttctact attgaaatcg tttggtctat aaaagattta
     5281 gtatgtctgc ttggaagtct cgctttttca ggagctatga tattgcttta tattcacttt
     5341 tttcttgatc attggcgaag cttgtggcat cgacaaaagc tagctcggat gattctagag
     5401 aaccattggt atgaagtgaa acaaactcaa agtgaaggct ttttcaaaga tttgaatagt
     5461 agccgaacca gagagactat cagttacttc cccaaaatct gttatcgaat gaaagatgga
     5521 ttactgtcta ttcgcgttca aatttcactt gggaaatacc aagatcagct tttaaaattg
     5581 gaaaagaagt tagaaagcgg actgtattgt gagttagtag aaaaggaact caaagactcc
     5641 tatgtggagt atactttact gtatgatatg atcgccaatc gaattgggat agacgaagta
     5701 gtggcagaaa atgggactct gcgattgatg aaaaatcaag tatgggctta tgattcttta
     5761 ccccatatgt taatcgctgg tggcacgggt ggagggaaga cctatttcct cctcaccatt
     5821 atcgaagcac tattgaagtc agatgcagaa ttgtttatcc tcgatcccaa aaatgcagat
     5881 ttagctgatt taggtacggt gatgcctcat gtttattcac aaaaggaaga aatttctgct
     5941 tgtgtggaag atttctatga acgtatgatg gctcgtagca aggcaatgaa agaaatgtct
     6001 aactacaaaa caggagagaa ttatgcctat cttggacttc cacccaactt tttaatcttt
     6061 gatgaatacg tggcttatat ggagatgtta acgacgaaag aaagtgcggt gattttgaat
     6121 aagctcaaac aaatcgtgat gttgggtcgt caatctggtt tctttctgat tttggcttgc
     6181 caaaggccgg atgcgaaata tttaggagac ggaatccgcg atcaattcaa ctttcgcgtg
     6241 gctttgggtc gtatgagtga actcggatat tccatgatgt ttggggaggt cgaaaaaaac
     6301 ttctttatga agcacatcaa aggtcgaggt tatgtggata caggtggcag tgtgattagt
     6361 gagttttata cgccacttgt accaaaaggg tatgatttct tagattcaat tagtaaatta
     6421 gaaaagatga atgattgagt gatcttcaat actgacttta tacttcaagt aacgtataat
     6481 acttttatat agtaggaaga aaggggataa ttaatatgga aagtaatgat tcttatttag
     6541 agtatgcaaa agcaaataag gataagttta tttcttatat tatcaaaggg aaatcccccg
     6601 aagttggacg agacgcagtt tttatggcag ggagtcctgg ggctggaaaa tcagaggttg
     6661 ccattggatt agctgagaat tatgataacc atgtgattat tgatgcggat tactttcgca
     6721 cccagtttcc tgattataat ggaaaaaact ctagtctctt tcaaaaagca tcttcttggt
     6781 tggtagagca ggcatttaga tatgtcttag atcatggcta ttcttttatt ctggatgcaa
     6841 cttttgcaat attaagtgct gaaaaaaata tctcgcgggc gttgaaaaaa cattttcgag
     6901 tgacgatttt ctatgtttat caagacccta aaatcgcttg gaaatttaca caagaacgtg
     6961 aaattgcaga gggacgtcat gtgccgaaaa aggcatttat taacgctttt ttcaaatcaa
     7021 gagagaacat tgcaaaagta aagagtaaac atccagatgt attgcttcat atcataataa
     7081 aagattatac gaataatatt tccgaagttc attatgctac ggataatcta gaattatttt
     7141 taccactagg gtatagtagg aaagatttgg aggttcaatt acatgactga gaaagaacaa
     7201 gtaacgaaaa ttgtaaagaa atacaataag agcattgcgg atttatcgga aaatgctact
     7261 gcaaaagagt tcaaaacggt gattaaatat gtagccgatc aggccaatga aaaacaacgg
     7321 aaattagttg gattaaataa aaaatagtag gacaaattaa taatgtatga gccaagagca
     7381 aaatgttctt ggcttttttc tttgcaatga atgaggtaat gtatgagatt tttagattta
     7441 ttcgccggta ttggtggctt tcgtttaggt atggaacagg cgagccatca ttgtatcggt
     7501 ttttgtgaaa ttgataaatt tgctagacgt agttataaag caattcatga tacaagtaag
     7561 gaggtggaaa tgcatgacat cacaagtgta tcagatgagt ttattcaatc cctcggacca
     7621 gtggacatcc tttgcggcgg atttccgtgc caagcttttt caattgcggg aaagcggcaa
     7681 agattttccg atactcgagg tactttattc tttgaaatcg ctcggttcac cactcttctc
     7741 cgacctaagc ttttattcct tgagaacgtc cgaggattgc tcaatcacga aggaggggct
     7801 acgttcgaga cgatcctccg aacgctggat ggattggggt atgacgtgga gtggtaggtg
     7861 cttaactcaa aggcctacat tccccaaagc cgggagcgga tattccttat cggacattct
     7921 cgagacgcat gtaccgaaca agtatttcct atcattggac cgtcttcaac atctgatcaa
     7981 aacatcagaa atttggtaaa tatcaatcct tccaatcgag gaatgggtgg acaagtctat
     8041 ggttcagatg gcgtagcgcc aaccttaaca ggtgatgaag gaatcaaaat cgcactgcca
     8101 gcaagggatg gaatctctgt agcaggacta ttaccgggaa agtttgaaca aggtaatcga
     8161 gtttatgaag ttactggaac cgcccccacc ttgtcaacga aacaaggtgg tacaaaaatc
     8221 atgattcgaa aaaatgaaac agaatatcaa gaggtgaagc cagaagacag tgtgaatctg
     8281 gcttttccta attccacaag tcgccgaggt cgtctgggaa aacagagtgt tcatacctta
     8341 ctaacaggag accaacaagc gatcgtcacg gatcagtatc aaattcggaa gctaacacct
     8401 agagagtgtt ggcgacttca gggctttccc gattgggctt ttgatcgaga ttctcaagtc
     8461 aattcagata gtcagttgta taagcaagcc ggaaactctg taacagttcc ggtcattttt
     8521 gatattgcca gaagattaaa agagttggat gtacatggtt tatgatgagt tgattggaga
     8581 aatttattgg gtgattggaa agattcagtc agacccagaa ttagaagaac aattgcgacg
     8641 gttaaaattt gatattcgca agaatggtgt caaagtcgct ggcgatccat atttaatgaa
     8701 tgaggagacg gatgctcgaa ttgaaataaa tcaagtaatt gcagaatttg agcggattgc
     8761 ggaccaagca aaagaaccag atattcgtca atacttgtta gaaatgaaag cagagttgga
     8821 agtaaatggt attacagatg agtaacctaa accaacacaa ctgactgtta ggcgctgacc
     8881 gcggaggatt cgcgaagcag aaatcctccg tggtgctgtg cctgttcatg gacggcgtta
     8941 gccgaccatg atgtctcacc cccgtatcta acaggggggt acaaaaacac aaaaaataag
     9001 taagcaacca ttaagaatgc ttgttgtatc aaagatagta agtaatttgt ataggtgtta
     9061 actcatttga aaaagttaac accttgaatt tggaggtgag aacttggcac aacggaattt
     9121 agattatcgg ctattaaaag accgacgcaa cgaatacggc gtttcacaaa ataaattggc
     9181 tacggcttgt ggacttagtc ggccgtactt aaatcagata gaaaatggtg gcgtaaccgc
     9241 atctataaaa accatgagga aaatttttga ccaactggaa agctttaatc ccgatttgcc
     9301 tttgacgtta ctgtttgact atgtaaggat tcgctttccc acaacggatg cacgaaaaat
     9361 tattcaagaa attctccatt tgaaatttga gtatatgctc catgaagatt atgcctttta
     9421 ctcctatcaa gaacagtatg tcatgggtga tattgtggtg atgttgtctc atgaagaaga
     9481 taaaggtgt
//
LOCUS       DBNICI010000109         9429 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.110, whole genome shotgun sequence.
ACCESSION   DBNICI010000109 DBNICI010000000
VERSION     DBNICI010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.110"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(56..1087)
                     /gene="queA"
                     /locus_tag="AC1ZCC_002064"
     CDS             complement(56..1087)
                     /gene="queA"
                     /locus_tag="AC1ZCC_002064"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HIQ1630302.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1280..2455
                     /locus_tag="AC1ZCC_002065"
     CDS             1280..2455
                     /locus_tag="AC1ZCC_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HIQ1630303.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            2459..3100
                     /locus_tag="AC1ZCC_002066"
     CDS             2459..3100
                     /locus_tag="AC1ZCC_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1630304.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3097..4014
                     /locus_tag="AC1ZCC_002067"
     CDS             3097..4014
                     /locus_tag="AC1ZCC_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HIQ1630305.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4011..4682
                     /locus_tag="AC1ZCC_002068"
     CDS             4011..4682
                     /locus_tag="AC1ZCC_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1630306.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5223..5711)
                     /locus_tag="AC1ZCC_002069"
     CDS             complement(5223..5711)
                     /locus_tag="AC1ZCC_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HIQ1630307.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(5912..6178)
                     /locus_tag="AC1ZCC_002070"
     CDS             complement(5912..6178)
                     /locus_tag="AC1ZCC_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HIQ1630308.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6178..6441)
                     /locus_tag="AC1ZCC_002071"
     CDS             complement(6178..6441)
                     /locus_tag="AC1ZCC_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIQ1630309.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            6962..7102
                     /locus_tag="AC1ZCC_002072"
                     /pseudo
     CDS             6962..7102
                     /locus_tag="AC1ZCC_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(7244..9067)
                     /locus_tag="AC1ZCC_002073"
     CDS             complement(7244..9067)
                     /locus_tag="AC1ZCC_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIQ1630310.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
BASE COUNT         2807 a         1977 c         1794 g         2851 t
ORIGIN      
        1 cataaaatct ttgtaaagct tttttgttat tcaaaactag cggcttttca taaccttatt
       61 tcacgaacat cgcatctcca aaactaaaga aacgatagcg ctcttcaatc gcatgctggt
      121 aagcagataa agtcaattct cttcctgcaa aggcactaac taacatgact aaagtcgatt
      181 ttggcaaatg gaaattagtt gagaaagctt ctaccacttt gaattcatag ccaggcgtaa
      241 tgaaaatatc agtccatccg ctgtctgctt tgatttctcc attaaatttc gttccaatcg
      301 tttctaatgt tcgaattgaa gttgttccga cagccacgat tttcccgcca tttttacgta
      361 cttggttcaa tcgctcagcc gcttcttccg tcaaacggta gaattcacta tgcatatggt
      421 gttcttcaat tttatctacg ctgacaggtc ggaatgtacc taaccctaca tgtagtgtca
      481 gatagactaa ttcaacaccc tttgcttcga tctcatcaag taattctttc gtaaaatgca
      541 atccagctgt tggcgctgca gcagaaccat tttctttcgc atagacagtt tgatagcgat
      601 ctgggtcttc cagacgttct ttgatatatg gaggcaatgg catttctccc aatgattcaa
      661 gaatctctag aaaaattcct tcatactcaa agtcaatgat ccgtccgccg tgttccaatt
      721 cttctttcac tactgcttgc aatctgccat caccaaaaga gattcttgtc ccaactttgg
      781 cacgtttcgc aggtttgatc aatgtctccc acgtatctcc ttgtgtattc gttagaagaa
      841 ggacttctaa atgcgcacca gtatctggtt tttctccata taaacgagca ggcagaactc
      901 gtgtattatt cataactagc gcatcaccag gctgtaactc ttcaatgatt tcatggaaat
      961 gtttatcttc catctctcca gttttccgat ccaaaacaag taaacgagaa tgatcgcggt
     1021 cttttaatgg tgtttgtgcg attaattctt ccggcaagtc aaaatcaaaa tcttctgttg
     1081 ttaacatatc gtcactcact ttctatcatg tgcttcatca ttctatcatg tttttcgttc
     1141 tgttggcaaa atcagcactt tttcacttct attttttgtt ttcctaaata aaaaaactac
     1201 cctatttgta ttttttcatt gttttaggca tactagtaat agatagttct tcattgaaaa
     1261 tagaaaaaga ggtggcacca tgattgaatt tcaaaatgtg tcaaaaattt ataaaggtgg
     1321 aaaagtcgct gtagaggacg tcaatctttc atttgaaaag ggtgagttca tttgttttat
     1381 cggtacgagc ggaagcggaa aaacgacgac gatgcggatg atcaaccgaa tgactgatcc
     1441 aacacaaggg aaaattttga ttgatggaaa atccatccaa gaaattgatc cagtggaatt
     1501 gcgacgtcag atcggctatg ttatccaaag tattggattg atgcctcaca tgacgatccg
     1561 tgaaaatatc actcttgtcc aaaaattgtt gaaagtcagt caagaagaac ggaataaaac
     1621 agctgaaaaa atgattgatc ttgtggaatt gccccgtgag atgttagatc gctacccaca
     1681 tgaattatct ggtggtcagc agcagcgtat tggagtcgtt cgtgcccttg cagcgaatca
     1741 agatatcatc ttgatggacg agccattcgg tgcgcttgat ccaatcactc gtgattcctt
     1801 gcaagacctt gtcaaagatt tacaagagcg tttgggaaaa acgatcgtat ttgtaaccca
     1861 tgacatggat gaagcgatta aactagcaag ccgtatcgcc atcatgagtg aaggccgtgt
     1921 gatccaattt gatacaccag aaaatatttt acgtcatcct gcaaacgatt tcgtagaaga
     1981 gttgattgga gaagatcgcc tactacaagc caagccaaat gcgacaagag tcggcgaagt
     2041 tatgctaaat acagcaatca cgattacacc ggaaaaatca ttacaagaag caatcaaact
     2101 gatgagggaa aaacgtgtag atacgttgtt agttaccgat aattcgaatg tattaaaagg
     2161 atttatcgat gtagaaacgt tgaataaaaa acgaggaaaa gtatccagtg tcggcgacat
     2221 tttgaataaa catgtttttt acgtaaaaga atccgcctat cttcgtgata ccttgcaacg
     2281 cattttaaaa cgcgggctga aatacgtgcc ggtcgttgat gaacagaaca aagtcgtagg
     2341 tatcctgact cgtgcatcat tagtggatat cgtctacgat gtgatttggg gagaagatcc
     2401 cgcaactact gatgttgcag agtcagctac tcctgaagaa tctaaggagg tctgacctat
     2461 gcagcagttt tttcatcagc acggttcaga aattctctca aaaggttttg aacatcttta
     2521 tatttcagga cttgctttga tcattggtat catcattgct gtaccgcttg gtgtcttact
     2581 gacacgtttt ccaaaagctg ccacgattgt gattggctta accagtgcct tgcagacaat
     2641 cccttctctt gctttattgg cattgatgat cccgctattt ggcgtaggta aattacctgc
     2701 gattatcgca ctctttattt attcattatt gccgatttta cgtaatacgt acattgggat
     2761 gaaaaatgtc gattggaatt atcgggatgt ggcaaaaggc atggggatga ctgagttcca
     2821 atccattttt tccgttgaat tgcccattgc tatgccaacg atcatggctg gtattcgttt
     2881 agctgccgtt tacgtgattg catgggctac acttgcctct tatatcggcg ctggtggttt
     2941 aggcgatttg atttttagtg gactgaataa ttaccaacca gatctcattt tggcaggtac
     3001 gatccctgta acaattttag cactattggc tgattggctt ctaggtctct tggaacaccg
     3061 gctgacaccg atcgcactta gagaggagaa tgaagaatga aacgtatact aaaaatgacc
     3121 atggttcttt tctctcttct tatacttgcg agttgttcct tccctggtct tgccagcaat
     3181 acagatgaag acaccatttc gatcacaggt ggaatcactt cagaagcaca gatcttagca
     3241 agtctagttg ccggaatggt tgaacattat acagataaaa ataccgcaat catcaacaat
     3301 ctggctacta caactatcaa ccatcaagcg atgatgaacg gagatgcttc aatttcagcg
     3361 gctcgctaca caggaacaga tttaacgact acattgaacc ttcctcctga aaaagatccg
     3421 aaaaaagcgt ttgctaccgt gaaagatgaa tttgagaaaa gatacggtca aacgtggttc
     3481 ccgtcctatg gttttgaaaa tacgtatgtc tttcttgttc gaaaagacac cgcacaaaaa
     3541 taccatctat ctaaagtcag cgatctgaaa aatgtagcgg atgaattagt agctggtgta
     3601 gatacttctt ggatcaatcg taaaggagac ggttatgacg gcttccaaga aacttatggc
     3661 ttctccttca attcgatatt acctatgcag attggacttg tttatgacgc agtcgaagca
     3721 ggaaaaatgg atattgttct tggctactct acggatggac ggatcgccag ttatgatcta
     3781 gtcatgttgg aagatgatcg tcaattcttc ccaccatatg atgctgctcc agttgtagac
     3841 aacaagatat tagctgatac gcctcattta aaagaagcct taaataagtt aggtcatacc
     3901 atcagtacgg aaaaaatgca ggaattgaac tacgaagcag ataataatct agtggaacca
     3961 tcagtagtag cacaacgttt cttacaagaa aaccactatt ttgaagggaa gtgaagcaat
     4021 tggataaaat gaatgtcttt caacaatttt tctattattt ccaggaaaat ggctcctata
     4081 tctttgcaca atttatccgt cacttcctga tttctatcta tggtgtcctc tttgcagcag
     4141 ttgtcgggat tccggttggg atcatgattt ctaggagacg taaattagca aactgggtga
     4201 ttcgtttggc gaatatcatc cagacgatcc cttctctagc catgatctcc attttaatca
     4261 ttggattagg cctgggagtc aatgtcgtga ttgttactgt atttttgtat tccctcttac
     4321 cgatcatcaa gaatacgtat accgggatga tccaagtaga caaaaacatc cttgatgtcg
     4381 gcaaaggcat ggggatgact gctagacaac gactattcat ggtagagtta ccactctctg
     4441 tttctgttat catggcaggc ataagaaacg cgttagttgt tgccatcggg ataaccgcga
     4501 tcggtgcatt cgtcggtgct ggtggtcttg gagatatcat tatccgagga accaatgcaa
     4561 cagagggcac atcgattatt ttagcaggtg cattgccaac tgcattaatg gcgatcatca
     4621 cggactggct tttaggtatt ttggaaagac ggctagatcc agccagccgt acatcacgct
     4681 gatgttattg taaaaaacaa aagcttctgc gaccatttgt tggtcgcaaa ggctttttta
     4741 gttctgtcat ttttaaggca ataatccact ctgattcttc cctccttatg ggcaaagagt
     4801 tgcttttctt agctatttat ccattccgta tactccaact tcatactgcc aacgggttca
     4861 tatctgcatc caccgactaa gtttttttct tatccgctaa ggcattttgc caatccattt
     4921 tcccatcact cgtcatggaa ttcataccac atgatctcta aatactcgtc tgcttgttct
     4981 ttgaattggg catcaattct tacatccttt ggcgtggctt tcatttaagc cttttccatc
     5041 agtcctcgat tgttcgatgc cttcagctgc atcatcaaaa ctggtgactg ccgtatcggt
     5101 aacttcgatc atcttttata caatcgggaa cttgccatcc tcatccttgg ctaaaaacat
     5161 atctagccat tccagcttct cgccatggga ggtttgcggt tcctaaaacc ggtacacgat
     5221 tcttatggac tgcatccgtc gcaactagac ttggcgatac gatgatcctt caccagaaga
     5281 gcacccccgt aaaccaattg atcgatatat tgccaataag aaaagacatt ggcgtaaaag
     5341 atatcgatcc ctcgtgatgc attgccgttg atactgctat tcatggccga aagcgccacg
     5401 actttaactt cttccgtttg agtgacattc atgttcggca atgtcgcttt ggatacccgc
     5461 tctgctaaag gaacgacact ggtattgtat ttcgtatctg tatcttttcc aaaagaccat
     5521 gccaacgaat cctctgggaa ccaataagac gactccggtt gattgtccat tgcttttttc
     5581 aacatacttt tggctgtttc tcgataaacg acatttccaa ttgtcttcgc ctcactttgg
     5641 cgataatcga tggtcgttgc cactgccaac actaacaacg atgctccaat tttactgatt
     5701 ttccttgtca cacttcttcc tccttgtttt acataaaccc gtcaacattc cattcctaaa
     5761 ctcaaacaat gacccttcaa aggtggagat ataggaacaa attaaagaaa tcatgcaata
     5821 aatccaagaa ggttggtttt agaacaaaaa aagagtctcc tttttgggga gactctttgt
     5881 taactatcta aaaaggttgg ctcttctcac ttcactcata atgtgtccag gcagaataaa
     5941 tttctacaac tttattttcc tcatcaactt tgtaaaccac tcgatgctgt ctattcagcc
     6001 tgcgtgagta tctgccttca tgtgtaggtc tcagcttttc aaaagactgc gtgggcatat
     6061 atggatcttc cttcaatgtc tgaatcactt cttcaaattg tttctttaaa ttcgtttgct
     6121 tgatttttct taaatccact ttcgcagagt ttttgattgc gactgtatag ttactcatca
     6181 aagagcttcc caatcaatat catcgatatt cgtaaagcta ctgtcgtctt tttcgcggtc
     6241 tcgtacttta tctaaagtac ctgtttgctc aagaaaaagg gtttcttgaa tcgctctcca
     6301 gtcttccagc ccaatcaata cagcattgtt tccactacga tcactaataa tctcgatttc
     6361 agtatgattt tcatttacct ctttcaataa ctgataaaaa tttttacgag cagtgctagg
     6421 attcatcgtt gtattagcca ttgtctctcc tcctttttct gatataatta tagcgtactt
     6481 gattaagtac gtcaaaaaaa tacaataaag cacttaaatt cagcaaactt ttcctctttt
     6541 ttacatagag aaaaagaccc ctttttaggg aggatctttg tccacgctca cttcttactc
     6601 ctttacgact agcggtagcc aaacgtaaag taattggtaa caacgtatca tctttagcaa
     6661 aggtcgaatc caaagcctgt gagtctatgc aacaacacca atacacgaag gttcctcgtt
     6721 gcagtgtgac ttgattgata tcttgaataa cctgaggata cgcacgaaag atttgtaacg
     6781 gttattcaaa actgattttg gccgtcaagt cttctactgc ctcaaaaatg attgcctctt
     6841 tcgtctcctg tcattcatgt tcactatcga tttgatactt gcttagaccg ttgggtttgt
     6901 gaatcaccaa ttaaaaatgg cactgagtta tggtaacaaa aaatggtcac ttgacgaggc
     6961 tgagattcct acttatgaag acacatggca cttaagagtc gaggtaaagg aagaagctga
     7021 aaatatagga ggctgtacat tatttggcaa aggattcgga aattatgatc aagacatcaa
     7081 aatcaaaatg ttttacagct aaataggaag aataataaca tttcatacgc tgttcccatc
     7141 tttaatggct tatagagggc acctttagct gagaataaga caaaacagac cctctagctt
     7201 caattaagag ctagagggtc tgtttttgat tttaaaactt ggtttatttc ttcaaatggt
     7261 agctataggt gctgacaacg atattttcac gccaagacaa tcgcaatctt aaccgcaaac
     7321 ttgtttggtt atggagaata ttctgccagc gtctgtttgg tacaaattga ggaatcaaca
     7381 cagtagttgt atagttttgt ttcgctgcat ttttacttac gatatctaca taacggataa
     7441 ttggattttc aatcgagcga taagaagagt ggacgattga aaaacggaca tctgggaaat
     7501 gctttttaaa ttctgcttcg atttcttttt ctttttcgac atcttcgtct agcgaaacat
     7561 gcatcgcaac gacataatca ccgatcgaac gtgcgtaatt caacgcaccg atatttaccc
     7621 gtgtcacatt cccgactaat acaagaacgg tattcccgtc ataatcatgt agttttgcat
     7681 cattttctaa acgaagctgt tctgctactt ttttataatg atcatggatc ttataaaaaa
     7741 tgaacataac gattggcata atgatgaaga aaggccaaat gtcccctaac cgatagacaa
     7801 acaaaatcgc aatgattgca tatgagatga acgcacccgt aatattggca atcgatttgc
     7861 ttaaccatct ttttccttct tttttccatt tgacgaccat ccctgtttga gataaagcaa
     7921 atggaatgaa cacgccgata gagtataacg gaatcaagcg ttctgttgag ccatggaaaa
     7981 taaacagcaa tacgatcgaa cctagcgcta gtgtgataat cccattggaa tagcctagtc
     8041 gatccccacg atcttgatac atgtgaggca tgaatttatc ttttgccaga ttgtatgcaa
     8101 gaactggaaa agcagaaaaa ccggtgttag cagcaactgc taaaatcagc gctgtagcaa
     8161 attgaaggac gtaatacaaa ataccatgtc caaagacagc tttaccaatt tgagacaaaa
     8221 cagtgacttc tttttcagga acgatcccat accaatagtt gataaatgtg atccccacga
     8281 agaagaaacc taagatcaat gccatcatcg tcaatgtagc agcggcattt tttgctcttg
     8341 gcttcttaaa gaatggtaca gcattactaa tcgcctccac cccagttaaa gaggaagaac
     8401 cagaagaaaa tgctcgtaaa atcaaagcga tagaaacacc cggcacagcc gctcctggaa
     8461 gtgcagtagc ttgcaatggc acagcacctg tcacgatatt atataaacca acaacgatca
     8521 gtaacgagat cacaaggata aatgtgtaca ccggaaataa taggaacgat gcagattccc
     8581 tcagcccacg aagattcagc atcatcagta gcaaaacgat tgtcacagaa atagcgactt
     8641 gatgaccgta gagtgctggc accgctgatg tgatcgcttc agcacctgct gagacagata
     8701 ctgctactgt caacatgtag tcgatcaata aagagccacc ggaaatcaag ccagcattct
     8761 ttccaagatt ctcactgctg acgacatatg ctcccccgcc atgaggataa gcatggatga
     8821 tttgacgata tgatagcgtt aatgaaatca ataaaataat aacaaaagcg gcaatgggta
     8881 gcgaatacca gatggctgca gcagacaaag cgactaaaac gacaacgatt tgttccgtac
     8941 catatgcaat agatgataac gcatcagacg acaataatgc caaagcagca aatcgggtta
     9001 atttgtgctc atcattttca gcagatttta aaggcttacc tactaacaac ctttttaagt
     9061 aatccaattt ctaaactcct ttacacacaa ttttacttca agttttatag tcgaaaattt
     9121 atagtaaaca agatgttata gtacgctcac tgaccttaaa agtcaataaa ctttaagaaa
     9181 ataattggaa aaaaacagaa aaatatcaag cgctttttca aacgctttaa tacagcaaaa
     9241 atctaacatt tttttatggg ctttttcttt gaattctata ataaaaggat agaacttgac
     9301 ttagaaaatc aagttctatc ctttttgata tcttaacttt tggaagagag atgtaaggct
     9361 gctttctacc catcattctt actactcgaa gcatagcgca gttgtcacaa cctcttgata
     9421 aacggtgtt
//
LOCUS       DBNICI010000110         9350 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.111, whole genome shotgun sequence.
ACCESSION   DBNICI010000110 DBNICI010000000
VERSION     DBNICI010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.111"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..417
                     /locus_tag="AC1ZCC_002074"
     CDS             <1..417
                     /locus_tag="AC1ZCC_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HIQ1630311.1"
                     /translation="YKEKLNQDKTAQEYKEYYTKGYQTDIASIKISEKEMAFTFKNGT
                     TKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAFRYIQFSDHTIAPADSEHFHLY
                     AGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(486..1214)
                     /locus_tag="AC1ZCC_002075"
     CDS             complement(486..1214)
                     /locus_tag="AC1ZCC_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIQ1630312.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(1227..2507)
                     /locus_tag="AC1ZCC_002076"
     CDS             complement(1227..2507)
                     /locus_tag="AC1ZCC_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HIQ1630313.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2648..3250)
                     /locus_tag="AC1ZCC_002077"
     CDS             complement(2648..3250)
                     /locus_tag="AC1ZCC_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HIQ1630314.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3495..3701
                     /locus_tag="AC1ZCC_002078"
     CDS             3495..3701
                     /locus_tag="AC1ZCC_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630315.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            4013..4732
                     /locus_tag="AC1ZCC_002079"
     CDS             4013..4732
                     /locus_tag="AC1ZCC_002079"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HIQ1630316.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4813..5496)
                     /locus_tag="AC1ZCC_002080"
     CDS             complement(4813..5496)
                     /locus_tag="AC1ZCC_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HIQ1630317.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5714..7900
                     /locus_tag="AC1ZCC_002081"
     CDS             5714..7900
                     /locus_tag="AC1ZCC_002081"
                     /EC_number="3.6.4.-"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIQ1630318.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            8246..8656
                     /locus_tag="AC1ZCC_002082"
     CDS             8246..8656
                     /locus_tag="AC1ZCC_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HIQ1630319.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            8670..9026
                     /locus_tag="AC1ZCC_002083"
     CDS             8670..9026
                     /locus_tag="AC1ZCC_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HIQ1630320.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         3361 a         1531 c         1764 g         2694 t
ORIGIN      
        1 tataaagaaa aattgaatca ggataagaca gcacaagaat ataaagaata ttatacgaag
       61 ggctatcaaa cagatattgc ttcaatcaag atcagtgaga aagagatggc atttacgttt
      121 aagaatggaa caacgaaacg ttcaaattat cgttatgtag ggaaagaaat cttgacctac
      181 gaggcaggaa accgtggcgt aaggtatcta ttcgaagcag aagataagga aagtggcgct
      241 tttagatata ttcaattcag tgatcacacg attgcaccgg ctgactccga acattttcat
      301 ttatatgcag gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca
      361 ttttatccag atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag
      421 gcaaaaaaga accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt
      481 tctttttatg aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt
      541 gccaacgtga cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca
      601 taggatttat gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca
      661 tagaattgta attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa
      721 gtagaaaaat attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt
      781 ttgaagatat ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata
      841 tgctcgacat tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg
      901 atgttgcttt ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt
      961 cctaaaggat ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct
     1021 tctagttcga tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg
     1081 agaagttctt ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg
     1141 attctatttt tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata
     1201 taaatgggaa tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa
     1261 cgctggtttt caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc
     1321 aagataaaca agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt
     1381 ttcaactggt ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa
     1441 tgattcttct tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca
     1501 atcgcattgt ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc
     1561 agttcgattg gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg
     1621 agttttaatg acagtaaact acggatctca ggaactttca tattatccaa catacttaga
     1681 ttggccggaa aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac
     1741 cgtaatcctt ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg
     1801 taatcatgtt tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc
     1861 tgttgcatcc tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc
     1921 caataataaa ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc
     1981 aaagctaaga catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat
     2041 gtaaataata tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa
     2101 gtctctaacg atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca
     2161 atgtgaaaat actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa
     2221 tcgccgatgc ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg
     2281 taaaccaatt gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata
     2341 gcaattagag cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa
     2401 atcagaatga ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata
     2461 caaattgtgt aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc
     2521 cattgatttt taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat
     2581 tttgaattca gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt
     2641 taattgttta atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg
     2701 taataggaag caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt
     2761 aagaagctga gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct
     2821 ttaataatgg gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat
     2881 agcgaagcag taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag
     2941 tcaattgcag atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca
     3001 gacaaacgaa ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag
     3061 aaagaaaggt gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga
     3121 ccgctagttt tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca
     3181 ccatatcttc ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt
     3241 tatctttcat ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt
     3301 cttttgtttc ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct
     3361 tcgttttcca agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat
     3421 gacaagtttg tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg
     3481 atggagggat tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta
     3541 aatctgattt atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg
     3601 tgatgatacg tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact
     3661 ggcagttagt tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag
     3721 agcataaaga ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa
     3781 acagcttttc atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg
     3841 tttatttgaa aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg
     3901 agttactttt ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt
     3961 tatttctata aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat
     4021 aattgtgatc attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct
     4081 tgcccaagat ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc
     4141 gctattttat tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa
     4201 acgctttttg gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat
     4261 ctacgaagat agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga
     4321 agtaaagcag tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc
     4381 acaaaagaaa ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag
     4441 aatcaaaaaa agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta
     4501 ctataaagaa ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa
     4561 aatagtgatc gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat
     4621 cgtacaagaa atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat
     4681 ccgctacact acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa
     4741 ctaagcaaat tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt
     4801 gattcattga gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt
     4861 ctgagtgagg gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa
     4921 cagatgatga cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag
     4981 atagtggaaa aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt
     5041 cgttgctacc ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat
     5101 tttatcaaaa gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc
     5161 gggatgctca tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag
     5221 aacaaacaag cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt
     5281 tagaagaatc cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca
     5341 ttctccgtca aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa
     5401 agcaccatat tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag
     5461 tcctattgta cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata
     5521 tttttgtctt ccttatctta gcataaacga ttgaaaaaaa ggaaaaattt tcagaaaact
     5581 aaaatcatat cgcttcccat tttcattttt agaaaggtct gttagtataa agaaaaaata
     5641 gacaacgaaa ctttaaataa ccaacttgaa ataaacaaat tcaaagaaga aagatgggaa
     5701 ggaaatcaat cagttgaatg aaaaagaaat tctccgatta caaaaagaat tgaaacgctg
     5761 ggagaacaag tataaaaagc ttcaacaagt ctttcggcta caagaagaaa tcattgctac
     5821 ctatgaagcg gcttatggcg aagcacccgg acttattgag gaagaagtac aagtaaaaga
     5881 aagtgaagga aacagctctt tctttaaaat gatggagaaa cgttcctata atcgcttcaa
     5941 tcaagaacaa aaagcagcta tcacatatga tatgaagaaa catcttcgaa tcatagcagg
     6001 agctgggagc ggcaaaacgc aaacaatctg tgcaaaagct gtttatctga tgacgcaaaa
     6061 acaagtagac gaagaacgca tactaatgat cacttttaca aggaacgctg caaatgaact
     6121 aaaaaaacga gtagataatt tcagccagag aaaaaccgct gtccatatcg gaacattcca
     6181 tagtatcttt tttaggatat acaatgaaat ttgtcgaaaa tttcctgaag tagcaatgca
     6241 aggaatacaa ggagactttt caaaggatac tgcacaaaaa gtgaatgctg ttttgcaaca
     6301 gctgatacgt aaatataatc tttatatatt tgatcaatat ggagaaaaaa cgattgcttc
     6361 tcggttagac tattggcaga atatgaacct ttcagtagaa gaaatggttc agttagttaa
     6421 agaaaaatat gattccattg ataagaatgc acgacagccg atatcagaac gactgtatgg
     6481 attgctcacg gaactccaag aacaaaaaag aagccagcaa ttgctagagt tcaatgatgt
     6541 actccaaaat ctgaaaagcg ctttggagaa tgaggagatc cagcattata tcggccaaaa
     6601 atatgattat ctattcattg atgaatttca agatactaat ccacttcaat gggaaatcgt
     6661 gaaacggatg acaaaggaaa acaggatcaa gttgattgtt gttggagatg atgatcagag
     6721 tatctatgga ttcagaggat cagagccagc atacatcaag aactttgaaa aagaatatcc
     6781 taccaaaact cttttcttat taactaatta tcgttctcgg gcagctattg tccaaggagc
     6841 aaatagattg atcagctata ataaaaatga tcggatacca aaggcgatga ttccagcaca
     6901 aaaagaagca ggtgttatgg aagcctattt gttttctgat actaaagcag aaagtcagtg
     6961 gctaatcaaa caaattcaga catttattca aaaaaatggg aaatataagg aatcaatcat
     7021 attataccga tcagcttcac aaacgcagca gcttgtccaa tctctgctga aaacgaatat
     7081 tccttttgta ctggaagcag atagtccata tgaaggtatc tttggtatca agtctttcca
     7141 acatttctat caaaaaattg ttcattggca aacagcactg aatctgcaaa agaaaaatca
     7201 ggcctaccag caattgctcc ggcagctgat ggcagactgc tacctgaaaa aaagtgaatg
     7261 cgatcaatac ttttctccta aatatcaaaa tatagccgtt gcagattata ttttgtcgat
     7321 aagccccaat ttaaaatcga aatctgcaga aatccgagag tttgaggaag cgttgcgtaa
     7381 aacagaccag catcaagaaa tctattcgct tgttcaaagc tatcttcatc tgccacgtat
     7441 agctaaggaa atcgaccaga gtgatcaaga atggatacga gaagaagtaa agcagcatgc
     7501 tactttcaga agactccaag ccttatcgca agaaacccaa gcgacagcta aagaattaaa
     7561 aacccgcttg attgcttaca gacaaggaaa acttgatgca ctatgtatcc agagcattca
     7621 caaaagtaaa ggtcttagtt accgaaatgt ctttttgatt ggttgtaacg aaggaagttt
     7681 gccttataac ggagcgacag aaaagaaagt aatcgacttt aatgaaataa aagcagaacc
     7741 agtaacgacg atagaagaag aacgaagact attttatgtt gcgatgacta gagcaagaaa
     7801 tcgtctgtat ctatgtgtac ctcaaatgaa aaattcaaga aaattgaaag tatcgagatt
     7861 tatcaaagaa accgggagtc gtgtgaaaaa aggaaagtga tagaagagag ggtgtgacag
     7921 aagtggtcag tttcaagaaa gaagaaggaa tgtacgaaaa ttgcttctca aatttttgtg
     7981 aaatttcagc ttattttcga agaagctatt tctggaacac agtttattcg gaaaagggag
     8041 gtgtgacaag aattttgtca cacctccctt atttattttg aaaatgagaa tactattatc
     8101 aaaatagaga atatatagaa aaattctcat tattcaaacg taaataatca gttattttga
     8161 ttttttttat attttattag ctaataattg gtattttaaa agaaattact gtagaattgg
     8221 attataatta aaaaggagtg agaaaatgat aaaagtatat acttcgccta gctgtacttc
     8281 ctgtagaaaa gcaagagcat ggttgcaagc gaatcaactt gaatttgaag agaagaatat
     8341 ttttgccgaa ccgttaacag aaagtgagat caaaaagatc ttggcagcga ctgaaggagg
     8401 agttcaagat attatttcca ctcggtcgaa aatttacgaa aagctgggca tagattttaa
     8461 cgaattaact ttaaagcaaa tgatcgtctt attcaaagaa tatccttcat tattgagacg
     8521 acctattttg attgatgaaa aaagaatact aatcggattt aatgaagatg agatccggtc
     8581 atttgtccct cgtgaaattc gatgtgttgc cagaaatctg atctatttac aaaacaataa
     8641 catcttaaat tcatagggag ggagccttcg tggaagtgaa agtctatgga tcagcgcact
     8701 gtgtaacaag tctgaaggcg aaacaatggt tagaacacca tcaattgcct tatcaatttg
     8761 ttgacttaga agaacgcgat ctaagtacag aagaagcaga agaattatgc agttttgaag
     8821 aagtgcaagc agaacagcta tttgcgactt ggtctgaggc ttttcacaac attgcgattg
     8881 acctttcctg tgtaggaaaa aaacaactga tttttttatg cacacaacat accaatctcc
     8941 tcagacgtcc tttgattatt atcgatgaag ttctatttat tggatacaat gagcagttga
     9001 tggaacagct tatcttaaat aaatagactt taactgtttt aatcaagtca ctgagcattt
     9061 catacagttg taacagtcga acacttagca tcaagcgtgt tatgtgttct ttttttgact
     9121 ttatttttct actatagaga catgctcaat ttatgtggtc aaaattcaaa tctaggtggg
     9181 gatatagagt taaaaatata ttgacatgtg taaatttaca tgatatattc atttctgttg
     9241 ttttaagtag caaagcaata aaatagtatt ttaaaaaaca tataaaatag ttgttgacat
     9301 gttagaaatg acatgataag ataactgagt tgttaaaaaa gaaccacttg
//
LOCUS       DBNICI010000111         9301 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.112, whole genome shotgun sequence.
ACCESSION   DBNICI010000111 DBNICI010000000
VERSION     DBNICI010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.112"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(99..2225)
                     /gene="pnp"
                     /locus_tag="AC1ZCC_002084"
     CDS             complement(99..2225)
                     /gene="pnp"
                     /locus_tag="AC1ZCC_002084"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIQ1630321.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(2413..2682)
                     /gene="rpsO"
                     /locus_tag="AC1ZCC_002085"
     CDS             complement(2413..2682)
                     /gene="rpsO"
                     /locus_tag="AC1ZCC_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIQ1630322.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            2933..3496
                     /gene="def"
                     /locus_tag="AC1ZCC_002086"
     CDS             2933..3496
                     /gene="def"
                     /locus_tag="AC1ZCC_002086"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIQ1630323.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(3749..4270)
                     /locus_tag="AC1ZCC_002087"
     CDS             complement(3749..4270)
                     /locus_tag="AC1ZCC_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HIQ1630324.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(4273..5115)
                     /locus_tag="AC1ZCC_002088"
     CDS             complement(4273..5115)
                     /locus_tag="AC1ZCC_002088"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HIQ1630325.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(5108..5833)
                     /locus_tag="AC1ZCC_002089"
     CDS             complement(5108..5833)
                     /locus_tag="AC1ZCC_002089"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HIQ1630326.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            6009..6305
                     /locus_tag="AC1ZCC_002090"
     CDS             6009..6305
                     /locus_tag="AC1ZCC_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630327.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      6561..6735
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            6873..8465
                     /locus_tag="AC1ZCC_002091"
     CDS             6873..8465
                     /locus_tag="AC1ZCC_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIQ1630328.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            8478..9218
                     /locus_tag="AC1ZCC_002092"
     CDS             8478..9218
                     /locus_tag="AC1ZCC_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIQ1630329.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         2581 a         1893 c         1843 g         2984 t
ORIGIN      
        1 gactgtgaca cggcttttgt cacagtcccc ttttttcgaa taattattgt ccccctgtca
       61 ccttcacgat cacaagtaca ttattcctat gaattaaatt atttttcttc atttttcgtt
      121 gaaccatctt cgttcaacat tgcttttctt gacagattta cgcgtccttg tttatcgatt
      181 tcagttactt tcacaaggat ctcatcacct agtttaacaa catcttcgac tttattgaca
      241 cgatcgtttg ctaattggga aatatggaca agtccatctt ttcctttgat caagttgacg
      301 aatgcaccga atttttcgat acggacaact ttccctagat aaacttcacc aactttgact
      361 tctttggtca aatcttcgat gatcttgata gcttttttga tcatttctgc atcagctgaa
      421 gcaatactta cttttccatc ttgatcgata tcgattttca caccagtttc atcgatgatt
      481 ccgttgatcg tatcgccgcc tttaccgata acatctttga ttttagcagg ttcgatctgg
      541 atcatttcga tttttggtgc atattggctt aattcttcac gtggagcagc aatcgttgaa
      601 gtcaattctt ctaagatttc catacgtgct tgttttgctt gagttaatgc ttcagttagg
      661 atttgttctg tgattccttg aatcttgata tccatctgta aagcagtgat accatctttt
      721 gtaccggcaa ctttaaagtc catatcgcca agatgatctt ccagcccttg gatatcagtt
      781 aggatcgtat agttttcacc atcagaaaca agtcccatcg caataccggc gactggcgct
      841 ttgattggta cacctgcatc catcaatgct aatgtgccag cacagatact tgcttgggaa
      901 gaagaaccat ttgattccaa tacttctgct actaaacgga tcgtataagg aaattcttct
      961 tctgaaggga ttacttgtgc taacgcacgt tcacctaatg cgccatgtcc gatttcccga
     1021 cgccctggag agcctgcacg tcctgtagaa ccaacagaaa attgagggaa attgtagtgg
     1081 tggatgaatc gtttgctgac ttccacccct aaaccatcaa tgatttggtg ctctcctaga
     1141 ggtgcgagtg tacaagctga taaagcttgt gtttgtcctc gtgtgaacaa acctgatcca
     1201 tgtacacgtg gcaataagct gacctctgat gacaatggac ggatttcatc caatttacgc
     1261 ccgtcaggac ggatcttatc gatggtgatc aattcacgta cgacatcttt ttcaagatct
     1321 tctgtgattt gtttaacttc tttcaacaag tgatcttcat cttcatgacc tgcaaatttc
     1381 tcagcataag cttcgcggat atcgatcttc acctgctcga tattatcttc gcgagcaagt
     1441 ttttcttcag tcattactgc tgctttcatt gtagcgtaag aagcatcaaa gatttctttt
     1501 ttcaatactt catcaacttg cagtaaagtc acttccattt tttctttccc tactgcttga
     1561 acgatttctt cttggaaagc aactaactct ttgatagcgt caaaaccaaa cagcaatgca
     1621 ccaagcatgt cttcttctga aacttctttc gcgccgcttt caaccatatt gatggcttgt
     1681 ttcgttccgg caacagtcaa ttcgatatct gtttgttctg cttgttctac tgttggattc
     1741 aagacgtact cgccatttac acgaccaact tctacgcctg cgattgggcc gtcaaatggg
     1801 atatctgaga ttgatagtgc taaagaagaa cctagcattg ctgccatagc tggcgagcag
     1861 tctgtttcta cactcatcac gatgttcgtt acttggactt cattacggaa gccttctgca
     1921 aacatcgggc gaattggacg gtcgatcaaa cgggcagtca atgttgcttc cgtacttgga
     1981 cggccttctc gtttgatgaa acctccaggg attttccctg ctgcatacat tttttcttca
     2041 taattgatcg ttaatgggaa aaagtctgta tcttttgctt ctttagaggc cacagctgca
     2101 cttaagacta ctgtatctcc ataacggact aaaacggctc cgtttgcttg ttttgctagc
     2161 tgaccgactt cgatttgtaa gggacggccg ccccatgtcg ttttgaatac ttgtttttct
     2221 gacataactt ctccttttcc ttctcgtgaa agcaagacgc tactaaacaa atgaaaactg
     2281 ccgaaagccg tcttttgagc agctttcatt tggttagtag gagtccatct agcttgatca
     2341 cggaatttta cttaattgat atacgagtaa aaaaagtgag attcatgtga atcccactct
     2401 ttttgactaa aattaacgac gcaagcctaa acgtttgatc aattcgcggt aacgttgaac
     2461 gtcagtttta cgtaagtaag ctaacaagtt acggcggtga ccaacttttt tcattaggcc
     2521 gcggtatgaa tgatggtctt ttttgtgcac gcgtgcatgt tcattcaagt ggttgatttc
     2581 ttcagttaat acagcgattt gtacttctgg agaaccagta tccccttcat ggcgagcgta
     2641 ttcgttgatg atttcattct tgcgttcttt tgaaattgcc atttctttca cctctttcga
     2701 ttattcccct gcgactgagt aaaacgttgg tgattcgctt aaccaagtgg caggtcatgt
     2761 gcctagagca cacagctata ctttacctga aaccgggttg aaaatcaagt agttaatcat
     2821 taagaaaagg tagttttcaa acaatcttac tactactttt ccatttttgt ggtaaactat
     2881 aatgcgaaaa tagacttgaa acacagtctt ttaagaaagg ggtacatatt ccatgattac
     2941 aatggatgat atcattcgtg aaggaaatcc gaccttacgc gaagttgcaa aagaagtttc
     3001 tcttccatta agcgaagaag atatctcttt aggaaaagag atgcttgagt tcttaaaaaa
     3061 cagccaagat ccaatcaaag ctgaagaact acacttacga ggcggtgttg gcttagctgc
     3121 tcctcaatta gatatttcta aaagaatcat tgctgttcac gtaccaagtc ctgacccaga
     3181 ggcagatgga ccttctctaa gtactgttat gtataatcct aaaattttga gccattctgt
     3241 tcaagatgct tgtttagggg aaggtgaagg ctgtttatct gttgatcgag aagtaccagg
     3301 atatgttgtt cgccatgcca aaatcactgt gtcttattat gatatgaatg gtgaaaaaca
     3361 caagattcgt ttgaagaatt atgaatcgat cgtcgttcaa catgaaatcg accatatcaa
     3421 cggtgtgatg ttctatgacc acatcaacga tcaaaatcca tttgcactaa aagaaggcgt
     3481 attggtgatc gagtagagaa ttggcaggtt ccttcgatac tatgctacaa gaaagcttta
     3541 taaaaaaggg gagtgctcaa aagctgcact tccctttttt atgtttctcc actaatcgaa
     3601 atttagatag acatctagaa atcgcaattc caaaaaattc tgtgtattgg atacgcatct
     3661 tttcttattt tttcttattt cttccatatg ggtgtttatc tcagagatga tagaatagtt
     3721 tctaggataa aaagtagcag ctacgatgtt atccttcgta gaggattcct gcttcagcta
     3781 gtttttgttt gatgtttttc agcatatgct tttctgggta gcggatcgta tgcagatgga
     3841 tgccgcctgt taattcggat aacaaagtag cattactttt ttgataacgt ctcataaagg
     3901 aatcgatatc ttgtggcgtt ttgatgtgca aagttcctgc taactcaccg taaaggggat
     3961 gttccacaat tacatcaatg atttcaccac cattttcaac gattagtctc agttcttctt
     4021 ctgtttgatc ttttccatgc tggacagcga ttttactgat gtgcccagta ttggtttctt
     4081 ctgtttcgag gatataacct cttgctgttg ccacgatact tttacctgca gctcgtagta
     4141 aagcaatatc gcctacaatg atctgacggc tgactccaaa agttttcgca aacttgctag
     4201 cactcactgg tttttctgta tttgccaagg tttgcatgat ttgttttcgc cgtgtctctc
     4261 cttcgatcac acttattcct ccttcatttt gttttggtat tcgcgcgagt gtttcatcaa
     4321 atcaacatac gtgtctgtta ttgtttcctt atatccctta tttttcagca gactaacaac
     4381 tttatccaat acttgctgct ctctttttgc atcataaact gcttggtttt cttctttttt
     4441 cacttgtcct attttagtta ccgtatccat tcttttttct aataaagcaa ccaactctcg
     4501 atcgatcata tcgatcttat gacgtaatgt ttcaagagac tcttctgtta gttcttcctc
     4561 tactgtcttg atgatacgag caggatttcc tgcaatcact acattgtcgg gaaaagattt
     4621 ggtcacaacc gaacctgcgc cgactactac gttgtcccct agagtgacac ccggcgtgac
     4681 gataactcct ccgcctaacc aaacattatt accgatttta atgggttttg cgtactccag
     4741 accgctgtta cgtttcactg gatgtagtgg atgcgtcgcc gtgtaaagct ggacattcgg
     4801 agcgaacatg cagttatcac cgatttcaat cgtgctgaca tctaaaaatg tacaattgaa
     4861 atttgcataa aaattctccc caacaaatat attatatccg taatcaaaat tgataactgg
     4921 ctccatatag atttttttcc ctgtacggcc aaaagtctct ttcaacaatt gggatcgcag
     4981 ttccgctgat tctgcttggt tgatgatttg gctttgactt cttgcaaaca tacggtcggc
     5041 aaccagctca ggatcattgg caaaataaag ctcgcctgca atcatttttt ctttttcggt
     5101 tttgaattca gtcataatct gctcctaatt cttgtatttt tttgaaaaca accgctccgt
     5161 cttcttgaat aaacgtataa ggctccgcta aataaacagg cgtttgattg attactaaga
     5221 ttttggctga cggctggcga aactgtacca gatcacaaaa cggatgaact tgaaaactcg
     5281 ttccaacaat gacgatcagc tctgcttggc tgacggcata agccgcctgc tccatgactc
     5341 catcttgaaa cccttcacca tataacacga tgtctgggcg tatttgtcct ccgcatgctt
     5401 gatgtttgtc gctgtgaaga tattctttcc agtctaccgt ttgttgacat tttcgacaat
     5461 gacattgata cagactccca tgaaaattga cgagttgcgg gcttttagcc tttgcgtgca
     5521 aaccgtcgat gttttgggaa acaacccaaa catttttttc tttcgctaga tgtgctattt
     5581 ccaaatgaat gatattgggt tttgcttcag gatgataaat ctttttgaca aattgataga
     5641 atttatccgg ttcattcacc attgcttgat gactcagcaa gtattccggc tgctccatcc
     5701 cgtggtagat tcctgacaaa gatcgatagt ctggcactcc agaaggtgtg gatacccctg
     5761 caccggtcaa aaatgtaatt tgctgataag ctaatatctc ttttgctgct tcgtctactg
     5821 ttaattgttt catgttttgt cacctcactt tccattatag cccatactcg aacaaattta
     5881 ttgttgaaaa gtcagaaaaa tgggaataaa aaagtgttat gggcatttga gcaatttatc
     5941 acaatgttca ctgacaaaag aatgaaaacg tgttacaata ctgctgtata aaagtgaggt
     6001 gaatcgatat gttaccattc tttatttatt ggggcgttat tctggcttgt atcgcctggt
     6061 tagcgttaag cgtttatttc tctgtttttt atcttgtacg caaagaaaat ggaaatcttt
     6121 gggcttttgc tttattcaac gtgattgccg caattgtttt agccattaca ttagccgtat
     6181 atcgaacatg gggctgggga atcacccaat actcaagttt gatttatcta atattagcca
     6241 tttatggcgt cgttgttctt ttgcaagcga tcctaggacg tgagccaaag aaagcggcag
     6301 cttaaatgat cgagatggaa ctggacacag gaaattttct gttctagttc tttttttatt
     6361 gtattacata catttttgtg tataattata ctatcgtttg caatggatag atgattcaca
     6421 ttttgattgt tttttcggta aaagtaaaga tttctatatt tatcagtatt ttttcaaaaa
     6481 ccagcagtta tagattgaca aagcctctcg agtatcgtaa actttttttg tataaataac
     6541 tgaatacgag atgcctcaat caatgaggta gaggtcgcga gaatcattaa ccctgtggag
     6601 tgaagcaaac atggagaagc aggaataggg aggatcgccg aaatgtgcac ttttttgctt
     6661 ggagtgtatg ttgggacgca agttaagagc ttgcggactg tcttagcagt gatgctaagt
     6721 tgcgctatgt tttgtgagag gtttatcacc ttatggaatt ttagcggcct ttttatatct
     6781 ataaatcggt cgctttttat tttttcagat aaagctttac cgcatttcat ctagaccgct
     6841 cgtgtatcat ataatcagaa agggagaaaa aaatgaaaaa aaatatggtt tcagttgccg
     6901 tactatttgc ttttgtcttc tcactattca gtgtgggatc tctggcaaat gcagatgaaa
     6961 aaacaccgag cgatgagttt cgcgtaggta tggaggctgg gtatgcaccc ttcaactggt
     7021 ctcaacaaac agatgagaat aatgccttgc caattcaagg ccaaaatagt tacgccggcg
     7081 gttatgatgt tcaaatcgct aaaaaagtag cagatggttt agggaaaaaa ttagtcatcg
     7141 tccaaacgaa atgggatggt ctggctcctg cccttcaatc aggcaagatc gacgcgatca
     7201 tcgccggaat gagccctact gctgaacgac gaaaagaaat tgattttacc gatccttatt
     7261 acgaatcaca attagtagta gtagttcaaa aagatagcaa atatgcaaaa gcaaaaagtt
     7321 tagcagatct ttctggagca aagatcactg ctcagttaaa tacattccac tatactgtga
     7381 tcgatcagat accagatgtg cagaaacaac aagcaatgga taatttctca gcaatgagaa
     7441 cagctttagc ttctggaatg atcgatggtt atgtcagcga acgtccggaa ggtgtaacgg
     7501 caactagtgt gaacaaagac ttggaaatgc ttgaattcag cggtgcagat ggcttccaga
     7561 caaacccgga agatgttcag atttctgttg gtatgcgtaa aggcgacccg gatatccaaa
     7621 aagtaaacca gattctttct ggcatctcta acgatgaacg aaccaaaatt atggaccaag
     7681 cagtcaaaga tcagcctgct gcaactgata gtgaagaaaa acctggcatc atttctgatt
     7741 tcactacgat ttggaaacaa tacggcaata tgttcttacg aggtgctgga cttaccttgt
     7801 tcatcgcatt gatcgggaca gttgtcggta ctacacttgg attactgatt ggtgtcttcc
     7861 gttcggtacc tgaatcagga aatgcactga ctcgcttctt ccaaaaaatc ggaaatgcac
     7921 ttctttctat ctatgtcgaa gtattccgcg gaacaccgat gatggtacaa gcaatggtca
     7981 tcttctacgg tctggcactc gcttttggca tttcattgaa ccgaacgatt gccgctttgt
     8041 ttattgtctc tctcaatact ggagcttaca tgacagagat cgttcgtggg gggatttttg
     8101 cagtagatga agggcagttc gaagctgcgc aagccattgg tatgacccat ggccaaacga
     8161 tgagaaaagt tgttattcca caagttttac ggaatatctt gccagcaaca ggaaatgagt
     8221 ttgttatcaa tatcaaagat acagcagttt taagtgtaat cggcgtagct gatttgttct
     8281 tccaaggaaa ctctgcttcc ggtgcgaatt tccaattctt ccaaacattt acgatcgtcg
     8341 ggatcatcta cttagtcatg acctttacga tcaccagaat cctgcgggcg gtagaaaaga
     8401 aaatcgatgg tccttcagct tataccaagc ttgaagaaat cgacaattca aatttgcagg
     8461 aataaggagg aagaatcatg aatgaaaaaa ttattgaaat caaacatcta agaaaaagct
     8521 tcggtgaaaa tgaagtattg aaagatatca gcgtaacagt caataaaggt gaagttgtga
     8581 cgatcatcgg ttcttccggt tctggtaaat ctactttctt gcgctgtatc aatttactgg
     8641 aaaaaccaac aggcggcgag atcatttata atggagaaaa tgtattagct cctaaatata
     8701 atttacctaa atatcgaacg aatctaggta tggttttcca atccttcaac ctgttcaaca
     8761 atatgaatgt cttggaaaac tgtatgtctg gtcaaacaac tgtcctgaaa cgagataaag
     8821 aaactgccaa aaaagtagca atcgaaaatc tggaaaaagt tggtatggat cgttatatcg
     8881 aagccaaacc agcacaatta tctggtggac agaaacagcg tgtcgcaatt gctcgggcat
     8941 tatcaatgaa tccagatgta ttgctattcg acgaacctac ttcggcgtta gaccctgaaa
     9001 tggttggcga agtgcttaat acgatcaaag acttagcaca tactggcttg acaatgctta
     9061 tcgtgacgca tgagatggaa tttgcaaaag atgtatctga ccgtgtcatt tttatggata
     9121 aaggtgtgat tgcagaagaa ggtacaccag aagatatctt tgtccatccg aaagaagaac
     9181 gtacaaaaga attcctttca cgtattttaa atgcttagga tcaatagctg attgggacaa
     9241 agagctgcgg tgtttaccgc agttcttttt tatgaattac tcataaaagg taaatttctg
     9301 a
//
LOCUS       DBNICI010000112         9256 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.113, whole genome shotgun sequence.
ACCESSION   DBNICI010000112 DBNICI010000000
VERSION     DBNICI010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.113"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(16..1638)
                     /locus_tag="AC1ZCC_002093"
     CDS             complement(16..1638)
                     /locus_tag="AC1ZCC_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIQ1630330.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            1887..2408
                     /locus_tag="AC1ZCC_002094"
     CDS             1887..2408
                     /locus_tag="AC1ZCC_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009268 - response to pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HIQ1630331.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(2594..4324)
                     /gene="spxB"
                     /locus_tag="AC1ZCC_002095"
     CDS             complement(2594..4324)
                     /gene="spxB"
                     /locus_tag="AC1ZCC_002095"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HIQ1630332.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            4489..5307
                     /locus_tag="AC1ZCC_002096"
     CDS             4489..5307
                     /locus_tag="AC1ZCC_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIQ1630333.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKHHFAVFGVMSGFIIMMILDVALG"
     gene            complement(5410..5607)
                     /locus_tag="AC1ZCC_002097"
     CDS             complement(5410..5607)
                     /locus_tag="AC1ZCC_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630334.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            5776..6354
                     /locus_tag="AC1ZCC_002098"
     CDS             5776..6354
                     /locus_tag="AC1ZCC_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HIQ1630335.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            6449..6586
                     /locus_tag="AC1ZCC_002099"
     CDS             6449..6586
                     /locus_tag="AC1ZCC_002099"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630336.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(7399..7902)
                     /locus_tag="AC1ZCC_002100"
     CDS             complement(7399..7902)
                     /locus_tag="AC1ZCC_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIQ1630337.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            8261..>9256
                     /locus_tag="AC1ZCC_002101"
     CDS             8261..>9256
                     /locus_tag="AC1ZCC_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HIQ1630338.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHY"
BASE COUNT         2796 a         1609 c         1769 g         3082 t
ORIGIN      
        1 aagctaaata ggccattatg cccataactc gttcaattgt ttttgaatat ctgcattttc
       61 caaaaattca tcatatgtcg tatcagctcg atcgatcaca cctttatccg aaacggcaat
      121 gatgcggttt gccaatgttt ggataaattg atggtcatgt gacgcaaata agattgagcc
      181 agtaaatgcc atcaagccgt cattcaatgc tgtgattgat tctaagtcta aatggttcgt
      241 tggatcatct aatacaagta cgttagcttt cgaaagcatc atttttgaaa gcatgcaacg
      301 gactttctct ccaccagata aaacattcac tggtttcaat acttcttcac cagaaaacaa
      361 cattcttcct aagaagcttc ttaagaaggt gttatcatct tcttctttac ttgcatattg
      421 acgcaaccag tctaaaatcg ttagatcagt atcgaattct ttcgttgtat cctttggtaa
      481 ataagaatta cttgttgtca caccccaacg tactgttcca gtatctggcg taatttcacc
      541 catgataact ttgaatagcg ttgttgttgt tatatcattt tctgcgacga aagcgacctt
      601 gtcttctttg ctcaaattaa aagatatatc atctaaaatt ttctttccat caatcgttac
      661 acttacattt tctacctgca ataagtcgtt tccaatctca cgttcaggtt tgaatccaac
      721 gaatggataa cgtctagaag aaggttggat atcgtctaaa gtaatttttt caagcatttt
      781 tttacgtgaa gttgcttgtt ttgattttga tgcattcgca ctaaagcggg caataaattc
      841 ttggagttct ttgatttgtt cttctttttt ggcatttgcg tttgcttgaa gtttagccgc
      901 tagttgacta gattccaacc aaaaatcata atttcctaca taaagtttga ttttaccaaa
      961 atctagatcc gccatatgtg tacatacttt gttcaaaaag tgacggtcat gggatacaac
     1021 gatcacagta ttttcaaaat tgatcaaaaa ttcttccagc caattgatcg attggatatc
     1081 cagaccgttt gttggttcgt ctagcaaaag aacatcaggt ttaccaaata gtgtttgtgc
     1141 taataacact tttacctttt gtccaccagt taattcactc atttgctgat ggtgcaattc
     1201 ttcagggatg ttcagacctt gcagtaaaac agctgcttct ggttcagctt cccatccatt
     1261 aagctcagca aattctcctt ctaattcagc tgctcgaata ccatcttcgt cagtgaaatc
     1321 ctctttcatg taaatcgcat ctttttcttt cagcacttca tacagacgct tgtgtcccat
     1381 gatgactgtt tcaagtacag tatagtcttc atagtcgtaa tggttctgtt tcaatacggc
     1441 catccgttca tctggtccca tagtaacgga acctgtggaa ggctgcagat cccctgataa
     1501 cactttaagg aacgttgatt tacctgcacc atttgcacca atcaagccat agcagtttcc
     1561 gggggtaaat ttaatattca catcatcaaa tagtttgcgg tctgaaaagt gcaaactcac
     1621 atcatttaca gtaatcaatt tttttcctca cttttatcat tattagtttt gacaagtctt
     1681 atagaattat ataggggaaa ggtaaaagat tcaaggcaat cgaacattct ttgtgtttca
     1741 cgtgaaacag aatgaaaaaa ataaaaataa aagcggagcg aaaatagcta ctataggatt
     1801 tattagtttg caacagcata atttttgacg ttatttataa gagggagtac tataaaactg
     1861 taaaagaaat gaaagaagga ttgaaaatgg atgaaaaaag aaccatcgac tggggctcat
     1921 tgattttggg aattttattt gtacttgtct cattgttatc atttcaagac cctgtcggaa
     1981 atttagttgc aattgtagta gtctttgcga tttttgcctt tattaaaggg atatttgaac
     2041 tgtttgtacg gaacagatta aaagagctaa caggatataa aggaaagatg cccttaataa
     2101 tcgggatcat cgatattcta gttggcgtct ttttcttatt caatattggt gcaggagtcg
     2161 cagcattgcc ttttgttttt gcagtatggt ttattgctga ttcagtttta gcgttattta
     2221 ctgctgactt ggcaagagga gtaagtgaag gatattattg gtttacgatc atcgtcaata
     2281 ttttaggaat tttattagga attttcttat tgtttaatcc aatttcttct gcgttgacat
     2341 taagcttttt agttggattc tattttatgt tatttggtat cacacatatc gtctatgcat
     2401 ttagataagt aaaatcaaac aaataaaaaa gccggcttcc gattacagtt ttactgttgt
     2461 aattggaagc cggctttttt atcatggaca aatatgcacg aggtgatttt taggtgtcga
     2521 gacaaattta tgcaaaaatg tgagaagcta tatacacaaa ttttatctta atactcgaag
     2581 catagcgcag ttgtcacaac ctcttttcat ataattcatt caatactggc aaatcatgga
     2641 cttcatattt ttctttgaat gcttggattt cttcttcaga atattttctt ggatctaaat
     2701 gcagatcttc tactggcaat gggcgcttgt tattgatctt cacatcgatt actacaggac
     2761 gatctgattt tgcagctgca tcaaaggcaa atcgaagttg cgagtattct gtgactgtaa
     2821 agctatttgc cccgagcgct tctccaactt taccgaaatc tgcatcttct aaaaatacac
     2881 caaatttctt ttgttccgta tcttcttgtt ctgcttcgat gaaaccaaaa gaatcattcg
     2941 aaaagacaac attaataatt ggaagctgat atttgacttg tgtaatgata tcttgcatga
     3001 ccatagcaaa cccgccatca ccacttaagg taaatacttg tttttctggg tagcttagtt
     3061 gtgcggcaat tcctcctggc acaccattac ccatagttgc gaaccatcca gatgtcgtat
     3121 gtttttgttt accgttcata ttcaataaac gaatagaatg gatagtcgtg ttaccgacat
     3181 ctgtgacgaa aatcgcatcg tcttcagcaa tacgattgat ttctttatag acagcctctg
     3241 gacgaatcgg ttcttcctct cgatcttcaa aagatttcaa ccaatttacc catttttctt
     3301 tattttcttg atttgctagg taccagctat cagcagggcg agcatcccct aactcgacaa
     3361 gtctttgcaa agctgtgttt gcatcaccga ggatcgacaa agatgtttca tggcgtcgtc
     3421 caaattttga cgcatcgata tcgatttgga taaattcagc agttggatta aaaaatgcac
     3481 gtccaaatgg gaagtcgcta ccgacaaata agatcagatc ggcttctgcc aatgcttcat
     3541 ttccgggttt agtagcgaca cgtccagcaa aaccaagaaa gttttcataa tcatcaggaa
     3601 taattccttt tgctagaacg gaagcgacaa caggaactga aaaatgttct gagaattctt
     3661 tgatttgcgg aaatccgttt cgcgttcctt gaccaacata aagaactggt ttttttgctt
     3721 tttcaagata aggcaaagca gctagcaaat ctttttcttc tggtaatatc acacttgttt
     3781 tgtgagtgtg agcggtggag aaaggttgtt cgtcaatttc ttcaaaacca agatcaacag
     3841 ggatagtcac gatagccacg cctttatgct cataggcggc tttaatggct tcatctacta
     3901 catgcggaag actttgtggt gtcatgactg tacgattgta tacgctaaca tctgcgaaaa
     3961 taggattttc atttagttcc tggaaagagt tatagttcat ggatgtagaa gcaacttgtc
     4021 ctaagaacgc tactagaggc acatggtcca tctgtgcatc atacaaacca ttgatcaaat
     4081 gtgtcgcacc aggtcctgca gagccgaaga cagcgccgac tttccccgtt aattttgcat
     4141 ctgcagcggc tgcgagtgcg ccgacttctt catgacgaac ttgaatgtag ttgatttcgg
     4201 ttttttcttg atatagcgca tccataattg aattgaaaga tcctccggga atgccgtaaa
     4261 tatggtcgat accccagctt tcaagaactt ttaccattgc gattcctgca ttgatttttg
     4321 acatggtgtt tcactccttt agcaatgatg atacataaat tttttgtatc tagagtatag
     4381 aactttttat cgaaaatccc aactaattcg tcttttcaca aaaatgtagt catgtttttg
     4441 ggaatatgct agagtagtac atagatagat tcaagagagg ggaatggcat ggttgaatgg
     4501 ctattgagac ttgaaccttg gcagcaagca cttgctggga cagcttttac ttattttatg
     4561 acagctcttg gtgccgggtt ggtttttttc tttaaagaaa tcaaaaaaga agtgctaaac
     4621 ttgatgttag gatttgcttc tggtgtcatg attgctgcaa gtttttggtc tttgttagat
     4681 ccggctatta caaaagcaga agaaaatgga gatattgctt ggctagttgt aagtattggg
     4741 tttggtttag gcggtgtgtt tttatatatg gcagataaaa cactgcccca tatgcatttt
     4801 ggaccacaac atgaagcaga aggattgcct acccatttaa aacggacgat cttgcttgtt
     4861 ttttctatta cattgcataa cattcctgaa ggtttagccg tgggggtagc attcggtgca
     4921 gcagcaactg cggataatcc gacagccgct atattagccg ctgtttcggt ggcgctaggt
     4981 attggtatcc aaaacttccc agaaggagca gctgtatcaa tcccgttgcg acaagaaggt
     5041 ctgagtcgta aaaaggcctt tgtttacgga caagcttctg gaatcgttga accaattgct
     5101 ggagtgattg gagcgctact agtaacgaaa gtggagctgc tgcttcctta tgcacttgct
     5161 tttgccgcag gagcaatgat ttatgtcgtg gtggaagaat tgatccctga agcacagcag
     5221 acattgtcca gcaaacatca ttttgcagtt tttggcgtca tgagtggatt tatcattatg
     5281 atgattttgg atgtggcatt aggataaata aaaaaaatat atatgatata tcaaataggg
     5341 ccgcgaactt atatcgcgac cctatttttt tagtaagtta tatcataatg aagtgaatgg
     5401 tatcatacat taataatagt tttttctatg tgtatttcct ttgatccagc gaacaagcca
     5461 gatgataagt gcgattggga ttagaacttt gaccgcaaac cataaaagcc cgcttaggac
     5521 actgacaaaa agactgatga cgaagctagc tgcaattagc actaaaagaa ccatgaagat
     5581 atttaagaac atattttttt gacgcataca aaaccctcct cttgttcatt ctttactata
     5641 gaaaaagtat aacagggatt gtttaaaaac acctccaact gaagactgat ttttacttca
     5701 tctacttaaa tgatcaaaca attgcttttt ctctggttta tcatctaaaa tagagcggaa
     5761 aaggaggaga atatcatgcc taaaaatcgt cttgaagcat ttacagatgc tgtaatcgcg
     5821 attatcatga ctattttagt tttagaactt catgaaccta acggagacac gtgggaggcg
     5881 ctcgctgcat taggttaccg attttttatc tatattgtca gttttatcat tttagcgatt
     5941 tattggaaca atcatcatca tttatttcaa gttgtacata agataaatgg acgggtctta
     6001 tgggcgaaca gtttctttgt ttttgcgctt tcgctttttc cgtttgcaac ttcatggatc
     6061 agtgagtaca tcaatagcct ggctccagaa atgacttatg gttttgttac cttgaccgct
     6121 aatattgcgt attatatctt ggcaaaagaa cttgtcaaag caaataaagg ccacgagaaa
     6181 gcattgaagg cgatgttgaa taattacaag aaatcgtatc tatcgattgg tttgaatgtg
     6241 ttaggaattg tcttagggta tttagtagcg ccagtagccg tgctgatcgc aaacatcgtg
     6301 attctagctg cttggatcat cccaagtcgt accatcgaat cacaatatcg gtaaaatgat
     6361 ccgtgtttaa aatttaagag aaaaatatag aaaaaataaa taaacgatca aatatccgag
     6421 tgatactagt attcaaagag ggatgcagat ggatcaaaaa agaattcatg taggactgat
     6481 tactagtttt gtcacgatgc ttatttattt ggatcaaggt catttgactt ggacagtagt
     6541 tggtgtcgta ggatttttga cattattcac agcaataaaa aaataaacat ctttatgtaa
     6601 tctgatgttc tatctattta agaaaagcat gtttacagca gtatgattct gttgtaaacg
     6661 tgtttttatt tgtggaaaat ttaattttta gaaaagtgta tcgaaaaata gatagttagt
     6721 ttttccacag tggataactt cgtaagagat aaaaataaaa aattagtcaa agaagggcgt
     6781 atagatttct tttgatagga gatatacaac agaaatgaat aaatattaac gtgttttcaa
     6841 acagtataaa acggtgtggt ttttggaata aaaaaataac atatctcatt ttacactttt
     6901 gttattttat acttaaaaaa taacgacaca gaaaataccg tgtctcagat ttctctacta
     6961 cttctaaaaa ctactgtttt tttcctttga aatgccttca aaaagtagga tcaggcagaa
     7021 tatactggca gaatcaagga gaagggagcc taaataagga gaaaaaccat agaaaatata
     7081 aagtaattac ttataaaaca agctttttat agaaaaataa tatacctgag tagggtataa
     7141 attaagacgg aattcctttc tagattgaga tattgactag gaagaatgtt aagattttaa
     7201 gcctttaagc taaaaaaaaa ccctttttgt taaataaggg gaatatagcc tgtcagtttc
     7261 aaactgcaag tcgtcctgct gcaataatat agtagattag agatgaaaat agattttttt
     7321 aaaccaatga ctgaataaaa agaccgtcat agatacacat tggaatgtgt ctattacggt
     7381 ctttttttac gaacgatttt attgaagacg gacagttcgg gtcaaaaata aaaaagctac
     7441 gatcagaaat aagaataaaa tgtatcccaa aataatggct agacggtcta acttatttgt
     7501 cgatagatgt ttgtttagaa gttgataggc ccataaacct atcagtcctc ctgttgtatt
     7561 agttatcaaa tcagtgatgt ccgctacgcc aataccgaat aggaattgaa tcaattctgc
     7621 caagaagctt acaaggaaaa taatccctaa ttttttagaa aaagaaagtc tcttgaaatt
     7681 gacattcaaa agcaccccaa aaggaaaaaa gacaatcaag ttataagtgg tctcacgtaa
     7741 taccgtcgta ttttcaaaag aaaaagtaga aaaagggacg agattgacat tgctgtaaga
     7801 ataagtgaga aattgtggaa gattaaatga tagcttgaat aaaatcaacc ataatagaaa
     7861 gaataaataa atccctaata aaaattttga tacataccgc atagacatct cctccattta
     7921 aaaagtatac catcgaaaac cgctgaaaaa tcataaaaaa gtatgaagtt taaggaccaa
     7981 cgtagttatc gtacgaaaat tagaagcttt tttttgttgg gaatccggtt tctttaacaa
     8041 agctaagata gcgagttgct tggaaaagtt gctatctttt ttctgtcaaa acgactcgga
     8101 atgttcgttt tttgttctaa tttctccaag ttgtttactt tttgatacta agtttatgaa
     8161 aaatcaagaa atagttgtta tactaggtaa tgctgacaaa cgggattggc atttcatctt
     8221 ggttcagctt tttttccaat tactaggaag gagggataga ttgttcaaga cttattttcg
     8281 tttcaataaa atacaagata atccttttcg tatcatgaat ctgatgctac ttatctttat
     8341 tgtattatgg ttaggttaca taaatcatga ttttttgatt tcaagttttt catcattagg
     8401 gatctttact tttttctatt atcaaaacat accgatgaaa tatttgctga aacgtatgtt
     8461 tctgatcggt tgcggactgt tggttgcttt cgttttgggg atcttatcga cttatgtttt
     8521 atggcttgaa ccatttgccg tagctttggt tgctttttcc agcagattcg tgttgcgatt
     8581 attccatatt tctaaaccag gtggcttgtt ttttgcgatg ctttctgcca tgggaacgag
     8641 catgcagctg ccaatcgctc aattaccgat cgtctcactt tattttttta tgggtgtcgt
     8701 ttttgccctg attgcggcag tgattacaaa attactggat tcacggcctg agcagacaat
     8761 tgaaaaagca actttaaaag aaagatttca cgaagagcct ttagtgatca ttgattctgt
     8821 tttctacagc gctgccttat ttctttccgt ttatgtcagt cacggcttga atctgcataa
     8881 tccttattgg ttgaccttgt catgtgcatc gattttgcta gcagaaaact tggatgcgat
     8941 gaagcatcga caagtccaat atctgattgg atcgatgggt gggttatgtg tgtcggcatt
     9001 cttgtcattt gtgccgttta cgcagttgca gaccattttt ctgatcactt ttctatatgg
     9061 aatcgcacaa tttctagttg ctagaaatta tgcagttgct aatatttttc ttaatccgat
     9121 ggcgttgatg ctatctacac ttattcgtgg agcatacttg atttctttga tcgaatatcg
     9181 ttttttaggg atcgtcatag gaagtttcat cggtttaggt gttgcgtggg taatgacggt
     9241 tggtttgcaa cattat
//
LOCUS       DBNICI010000113         9152 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.114, whole genome shotgun sequence.
ACCESSION   DBNICI010000113 DBNICI010000000
VERSION     DBNICI010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.114"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            278..787
                     /locus_tag="AC1ZCC_002102"
     CDS             278..787
                     /locus_tag="AC1ZCC_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIQ1630339.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="AC1ZCC_002103"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="AC1ZCC_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HIQ1630340.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="AC1ZCC_002104"
     CDS             2322..3302
                     /locus_tag="AC1ZCC_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIQ1630341.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="AC1ZCC_002105"
     CDS             3424..4842
                     /locus_tag="AC1ZCC_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HIQ1630342.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="AC1ZCC_002106"
     CDS             4913..6382
                     /locus_tag="AC1ZCC_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIQ1630343.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="AC1ZCC_002107"
     CDS             6392..6859
                     /locus_tag="AC1ZCC_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIQ1630344.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /gene="scrK"
                     /locus_tag="AC1ZCC_002108"
     CDS             6895..7767
                     /gene="scrK"
                     /locus_tag="AC1ZCC_002108"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HIQ1630345.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFIKSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7859..>9152
                     /locus_tag="AC1ZCC_002109"
     CDS             7859..>9152
                     /locus_tag="AC1ZCC_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIQ1630346.1"
                     /translation="MKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQLFSD
                     GKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLPLIIK
                     GNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKKGAFT
                     GAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEYANVR
                     LILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITIDSKIF
                     CSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWIDPEI
                     SIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESDEFIA
                     LRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIE"
BASE COUNT         3153 a         1349 c         1835 g         2815 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttaaatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaat
     7861 gaaagatgaa gtattagagt atatcaaaaa taatttaaag tattacgatt tttctgctca
     7921 agatattgca atgaaatttt gtattaaaag aaatgtcgct tcccactatt tgaatcagtt
     7981 attttcagat ggaaaattac taaagaatga ttctgttaga ccagtaatgt tcaagtataa
     8041 tcaacaaaag ccaaaagact gcttttctaa gtttattgga gcagacatat ctttgaaatc
     8101 cactatagat aaatgcaaag ctactgtaat gtatccccct aatggcctcc cattaataat
     8161 aaaaggaaac agtggcgttg gaaaaagttt tttagcaagt ttgatctatc agtatgctct
     8221 tgatagaaaa gtaattcata atgacgcaaa atttgttgtt gtgaattgtg cagattatgc
     8281 aaataatcca gagctattgt cagcagtttt atttggatat aaaaaaggag cctttactgg
     8341 ggctgaaaaa gatactgcag ggatgctatc ttctgctaat ggaggatacc tttttttaga
     8401 tgaggttcac aatctaagtg cagaaaacca agagaaattg tttcttctta tggattctca
     8461 aaagtatcgt aaacttggtg aaagtgtgca atgggagtat gcaaatgttc gccttatctt
     8521 agccacaaca gaagataaaa atacctcttt gttagcaact tttagaagac gcatcccagc
     8581 tgaaatcact ctgccagatt acaataatag aagtacatct gaaaaaattc aattattatt
     8641 tgaatttctt aaagatgaag caattacgat tgattcaaaa atattttgtt cgattgaact
     8701 ttttactgat cttttatcga aaacatttga aggaaatgtt ggagaattga ggaatgaaat
     8761 caagcttctt tgtgcagaag gttatttagg aaataagaga aagtcttcaa tttatttagg
     8821 ggacaaacta gacagcggtt tttggataga tccagaaatt agtatagatt taaaaaaaat
     8881 gtttcttaaa tcactttctg aaattgattt gattgaactt tttcaaaatc acatttcaat
     8941 tgaagaaagc atgagtcaat taagagatag aatacttaaa gagtgtgcag gttcttctga
     9001 gtataattat ttagaaagtg acgaatttat agcattaaga aactatgttg ttaataaaat
     9061 atcaccaatt attgattcga caggtctatg tttattagat gaattcttgg cggatatcag
     9121 tctatttata atgtttattg atgtaattga ag
//
LOCUS       DBNICI010000114         9091 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.115, whole genome shotgun sequence.
ACCESSION   DBNICI010000114 DBNICI010000000
VERSION     DBNICI010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.115"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..581)
                     /locus_tag="AC1ZCC_002110"
     CDS             complement(<1..581)
                     /locus_tag="AC1ZCC_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIQ1630347.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLL"
     gene            642..1895
                     /locus_tag="AC1ZCC_002111"
     CDS             642..1895
                     /locus_tag="AC1ZCC_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HIQ1630348.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            2217..2444
                     /locus_tag="AC1ZCC_002112"
     CDS             2217..2444
                     /locus_tag="AC1ZCC_002112"
                     /inference="COORDINATES: protein motif:HMM:NF013777.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIQ1630349.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            2567..2893
                     /locus_tag="AC1ZCC_002113"
     CDS             2567..2893
                     /locus_tag="AC1ZCC_002113"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HIQ1630350.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            2961..3674
                     /locus_tag="AC1ZCC_002114"
     CDS             2961..3674
                     /locus_tag="AC1ZCC_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HIQ1630351.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            3984..4834
                     /gene="licT"
                     /locus_tag="AC1ZCC_002115"
                     /pseudo
     CDS             3984..4834
                     /gene="licT"
                     /locus_tag="AC1ZCC_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            4955..6820
                     /locus_tag="AC1ZCC_002116"
     CDS             4955..6820
                     /locus_tag="AC1ZCC_002116"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HIQ1630352.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            6834..8277
                     /locus_tag="AC1ZCC_002117"
                     /pseudo
     CDS             6834..8277
                     /locus_tag="AC1ZCC_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            8385..9086
                     /locus_tag="AC1ZCC_002118"
     CDS             8385..9086
                     /locus_tag="AC1ZCC_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIQ1630353.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
BASE COUNT         3007 a         1495 c         1921 g         2668 t
ORIGIN      
        1 agtaaaaagt aagcatactt ttctaactct gtcttcaatt gttttaaaga agtattcact
       61 aatttatctt ctgtagttgt tttgaccata acttggtttt tcaaaccttt gatactttca
      121 atatataaaa tttcccgagg atacaggacg agacttccac ctttttgatc aataaagatt
      181 ttatcgacat ttacgatctg ctgttctttc caattgatct gttctagcac ttgacgaatt
      241 tgttcagata aattcctgtc ttttctgata tatccaatcg gaaggatttt ccgattgatg
      301 atctctactg caaaattcgt ataagcggtg aaaaaaacaa tattactatg tgcatttttt
      361 ttacgtattt gttcggcgac atcaatacct gtgagtgatt gcttcaagtc gatatcgata
      421 aagaaaacat cgtgattatc aatagtgccc aactggatca actgattgat ttgagagata
      481 gaagtgagct gcacgatatt ctcgatatga tgattgacca attctctgat gatccattcg
      541 ctgtgaaact ggttatcttc tatgataaaa tactttttca tatgcaggct cctattaaaa
      601 aaatatgtgt acattttgaa acaaaacaat ggaggaataa gatggtcgca acagtgacac
      661 tttatttgac ctttactttg attttagatt taggtatcta tttattcttt tcgaaacaac
      721 tgctagtaaa aaacagcttt ggttgtatag ctttgatggt ggtgacactt ttacttccta
      781 tccctaaagt gagctttcct tacctagttg gcttattatt gatgtttctt ttttatttta
      841 ctacgaaaaa cctgtttatc agctgtttga tcagtaatct tttttttgct atcactggac
      901 tcagcttttt tctggttttt gatctgatgg attatttagg aaagagacat cttggaacag
      961 caagtttttt tgcactactt atcgcattta ttttctatct attagtaaaa agtatagaca
     1021 aacagacgag atttctcaac ttgcttttta cttatccaac tgaacactat tttggaggta
     1081 tttgtatttt attaagctgg ggcgtgattt acttggtatt gggaagtgtt cacttccgct
     1141 catctaatta tctgcttcta tctcttttgt cgatagtcat cagttttttt tctttgtttt
     1201 tttccattat gttgatcagt ttccaagtaa aagaaagaca acatcaggaa tcgcttcaac
     1261 tgtatcaaat aaatgaagaa tattaccatc atctagagga attcaaacat gattataaaa
     1321 gtctactgtt ttcattaaaa attactcttc aggaaaataa aaaagaaacg ttggaatttt
     1381 tagaaatatt agaagatcat tctgcagcta tttttgaaaa accgcaaata caacagctaa
     1441 caaatattgt atccccagct gtcaggggtg tctttttaaa atttctagaa aaagcagaat
     1501 cggagaaaat cccgatccaa gtagttattc ctgtcgaagt gaaaacaatc ccgattgatc
     1561 tggttgtttt tattcgctgt ttatctattt ttatttctaa cgcattcgat catcgtgtag
     1621 ccgatcagag tccgattacg atcaatctgc tggaagaaaa agagggatca cgttttagta
     1681 tagcgaacaa ggcaaatcca acgaatttga cgctatcgga gatggtcaaa agaggaaaaa
     1741 cctctaaaaa aaatggcggg ttaggtctat acagtgcgaa aaaaatgttg gatgtttacg
     1801 aaaatgcaga actaaaaatc gaattttcaa aacagaatca ctattttttt gtagaaatgt
     1861 atctggcatc tagaaagagc aaatcagcct actgacttac tgtcaaagga taagtttgag
     1921 gaaagtctac acggagatag agacaacaac acagcatatt caaaagaaac agaagcgtga
     1981 tgaccgttgt cacccttctg tttcttttgc tatgtagtta aacaaagtag ttgacacttc
     2041 cgctacttag tattagtacg aaagtatact tcatgatact aagtagataa gaagtgaaaa
     2101 gcggaaccca cgatcgtcaa aatgctaact gctttgctta caccagataa agggaagagc
     2161 aatatcgctt attttaggcg tggagaagca agcaaatccc tacgatcaca aaaaaaatgc
     2221 tgacccagca gttaagagag ttagaacaag atgaaagtat ccatcgaaaa gcctataacc
     2281 aagtcccgcc taaagtagag tactcgctca ccgaagaagg aaaatcatta agagagattc
     2341 tagtagcgtt gtctgtttgg gaagaagaac gtatcgaaaa acaaaaagcc tcaagggaag
     2401 aagacacgct actgcataca catggtggct acctaaatta ttgattttct atgacattta
     2461 ttttaaataa aagaaaacag gagttttttt aaaagcatca tctatttttt atgtaaaaaa
     2521 atggtatgat taatcaagta agaagtgatt ggaagaaggc aagcaaatga atagtatgga
     2581 aaaagtcaac cagtttagag atgaacgtaa ttggcgacaa tttcataacg aaaaagattt
     2641 ggctatctct atttctttgg aagcatctga attgttagaa ctttttcaat ggaaaacgcc
     2701 ggaagaagta aaagaaacac aattagaacg aatcaaagaa gaactggcag atgtattgat
     2761 ctattcatac atgatggcag acaaccttca cctggatcta gatgagatca ttgaagaaaa
     2821 attgatcaaa aataatttga aatatcctgt agaaaaaagc gcaggtgtac gaaaaaaata
     2881 cacagagttg tagaaatgtg tcattcggct gttggaaaga gcaaaagaaa aacagtaaaa
     2941 tgagtctgga gggagagaaa atgaaaaaaa aaggaaaagc aaaaagatgg ctagtcaata
     3001 tcctcttgtt cctcttgtta ttagtaggac tagctttgat tttcaatgaa cagatcaagg
     3061 attattttgt cagagagaca ggagataaat atgctatagc gaatgtaaca aaagaagatc
     3121 tgaaaaagaa taacgataaa gatgtcagtt ttgactttga tgcggtagaa ccaatgacga
     3181 cagaaggggt catgaggtcg caaatgagag gaacagatct accagtgatt gctagtatcg
     3241 cagtcccttc agtttcaatc aatttaccgg ttttcaaagg cttggataat acgtcactac
     3301 tttacggagc aggaacatta tctccagatc aagaaatggg aaaaggaaat tatgccttag
     3361 caagccatcg tgcaacgaac cctgaattgc tgtttacacc gctagaaaat ttagagatgg
     3421 gtgccaagat ctatttgaca gatttagaaa atgtgtatac atacaaaaca ttttttaaag
     3481 aaaaagttgc tcctacagat acccaattac tgaatgaagt cgagggaaaa gaaattgtca
     3541 cactaattac ttgtggcgac atggacgccg tcacgcgact agttgtacaa ggagaactgg
     3601 aaagtgttac atcgataaaa gatgcgacag atgatatgag atctgctttc aatctagaga
     3661 caaaaacatt ttaggatact gggacataag tcgttacaga ttaaaaacag ccaaaggata
     3721 tgaatcgata aggaaaaacc aatttgctga aaaaaagaga aacgcaaaga ggtgcatctc
     3781 tgatttggtt gttttctaga tagacttgtg ttccagtttt tttatttttt atatgaaaag
     3841 gcttgcataa ataaaaattc gatggtatga tgattttgaa taagaaatgg attgttactg
     3901 tacggcaggc aaaaccagaa ttcagctaag aaacagtagg cgaattctag gtttttttat
     3961 ttttcttgag agggcgaagc aaaatgaaaa ttgataaaat tttgaataac aatgtcgtca
     4021 tttcgaaaaa tggattcggg gaagaagtcg tttgtatggg aaaaggacta gcttttcaaa
     4081 aaaagattgg ggacgagatt tcgccagaag cggtccaaaa agaatttgtt taagagattc
     4141 ttttgctaaa aaccaattcc agcagctgat ggctgatatt ccggcagagg agatcgaatg
     4201 ggtcaaacaa gtagtcgaac tggctgaaga taaactaaaa gttgaatttt cgcctaatat
     4261 ttatctgaca ttgaccgatc atcttcatta tgcgatcaca cgagcaaaag aaaatatcca
     4321 actgcctaat ccattattat ttgaaacaaa aaaattttat ccgaaagaat atcaagcagc
     4381 gaaagaggct cttttgttcg tgaaacaaaa gacgggtgtg gaactttctg aggatgaagc
     4441 cggttttatt gcctttcatt ttgtgaatag ccaacaagga aacgaagaca tgcaagtgac
     4501 gatgaccgct accacaatgg taagagatat actgagtatc atcagtaaat tcttcggcat
     4561 cgtgttcgat gaagaatctt tgaattatca gcgtatcatt actcatctgc agttttttgc
     4621 ccagcgttat ctcaaagggg aaggatctga cgagcaagat gaatttctct acgcattagt
     4681 tcaaggaaag tatccaaaag cgtttcattg tgtcgaacgg atcaatgagt atcttcgaca
     4741 aacgcaaaaa caggaaatgg ggatcgcaga gcagatttat ttaacgattc atattcaaag
     4801 agtagtaagt gagaaaaaaa gcatccaatc ataaggattg ttactgtatg gcaggcaaaa
     4861 cctggattga aaaaataggt tcacgaatgt ttgtgactgt ttttttcaat ctgggttttt
     4921 attttttaaa taaaaaagaa agaaggaaaa aacaatggat aatcaagcag ttggtcgtcg
     4981 tgtatgggaa gcggtaggag gacaaaagaa tgtcaaaagt ttagtacatt gtgctacgcg
     5041 gttgcgattc agattaaaag atgaatcttt agcagatacg caaaagttaa aagaagaccc
     5101 ggacgtgatc caagtcgtac aaagtggcgg tcagtatcaa gtagtgattg gcagtaatgt
     5161 agcagatgtt tatcaatcga tcgtggatga ggaaggtctt gcagtttcgg aggatggaaa
     5221 agaagagtcc aaaaatattt tgaatcgtct gatcgatatt atctctagta tatttacacc
     5281 ttttctaggg gcgatggcag ccgccggggt attgaaagga ttcttatcat tggcaacggt
     5341 gatgggctgg ctgacagctg atagcggtac ttatcaaata ttgtttgctg cagcagatgg
     5401 tgtctttacc ttcttacctg tcatgcttgc ttttacagca gctaaaaagt tcaaagccaa
     5461 ccagttttta gcagtagcaa ttgctatggc acttgtgtat ccagctatca cagcagttgc
     5521 tggtgcggga gaagctatca gtttctttgg gatcccagtg attctttcac catctggtta
     5581 tacttcttct gtcataccaa tcattctagc cgtatgggtt caaagtaaat tagagccatt
     5641 cgtaaaaaaa gtgattccgc aatttctgca aatgatttta gttccgcttg tcgttttagt
     5701 agtgatggtt ccattaacat ttcttgcttt aggaccaatc gggacggttg ccggtaatgc
     5761 tttaggcggt ctcttcaact cgatttatgg atttagtcca atcgtagcag gtctaatcat
     5821 gggaagttta tggcaagtat tcgtgatgtt cggcatgcat tggggattcg taccgatcat
     5881 gtttttgaat atcgaacaat atgggtttga tgtattgatg ccaatgcttc ttccagccat
     5941 cttggcacaa ggtggggctg ctttagctgt agcattgcga acaaaagata cgaaacttcg
     6001 agcattaggg atttcttcta cagtgacttc gttatttgga atcactgaac caactgtgta
     6061 tggtgtgaca ttacctttga agaaaccatt cattgccgct tgtatttctg gaggtattgg
     6121 tggagctatt atcggatttt ccggagtaaa agctttctcc agcagtttgg tcagcttatt
     6181 gacgattccg actttcatta atacggtgga tggtgtagag tcaaatgtga cagtagcagt
     6241 aatagctaca ggaattgctt ttgttcttgc attcgtaggt acattgattt taggatttga
     6301 cgaacaaaca caagacaatc aattagaaaa caaacatgcg aatgcaggag aaccgattac
     6361 ttcagcacgg cataccttaa aaagtccttt aacgggtaaa gtactgccat tgagtgaagt
     6421 accagaccaa gttttttctt ctggcgtgat gggaaaaggt atcgctattg atccagaagt
     6481 aggagaacta gttgcacctg cagacggaga gatcacgact atttttccaa ctggacatgc
     6541 tgtagggatc acaacgactg acggagcgga gattttgatt catatcggga tggatacagt
     6601 agaattaaat gggaatggct ttgagatctt agtcaaacaa ggggatctcg tcaaagcagg
     6661 agatttattg attcgttttg acatcgaagc aattagagca gctggctata gcgtaatcac
     6721 tccagttgtc attacgaata ctgatgcatt tgccgatata cttgaattag accaaaaaga
     6781 gattattgca aacgaagatg ttctggcaat tgtaaaataa ggaggaaatc aggatgcaat
     6841 cacgtttttc agaaaaattt ttatggggtg gagcaactgc tgccaaatca attagaaggg
     6901 gcttatgatc gagacggaaa aggactatcc gttgcagatg ctatgcctgg aggtaagcag
     6961 cgctttgcga ttatcggcag tgaagaattt gactggacga ttgatcaaga aaaatatatt
     7021 tacccgaatc atcgaggaat cgatcattac gatcgtttca aagaagatct tgccttgttt
     7081 gcggaaatgg gattcaaatg ttatcgtttt tcgattgcct ggacacgaat ttttccaaat
     7141 ggagacgaag gtactcctaa cgaagcggga ttagaatttt atgatcagct gattgatgag
     7201 tgcttaaaat atgatattga acctgtgatc acgatttctc attacgaaat gcctcttcat
     7261 ttggcaaaag aatacggtgg ctggaaaaat cgaaaactga tcgacttcta tgaacgattt
     7321 gctcagacag tattagagag atacagcagt aaagtcaaat attggatgac tttcaatgaa
     7381 atcaattcgg cctttcactt tccagcttta agtcaaggat tagtaaaaag caatggagca
     7441 ggagagtacc aaaatatttt ccaagcatgg cataatcaat tcgttgcaag tagcaaagcc
     7501 gtaaaaatcg gccacgaatt gcgttcggat atccaaatcg gctgcatgat tatctatgca
     7561 acaacttaca gcatcgatgc caatccagtc aatcaggcag caacaatgat ccaaaaccaa
     7621 gaattcaatt ttttctgcac ggatgtccaa gtacgtggag aataccctgc atacacagcg
     7681 cgtacccata aaaaatatgg agtcgatccg gaaaaattag agcaaacgga aaaagacttc
     7741 cagttgctaa aagaatatcc agtggactac atcggattta gttattatat gtctacggca
     7801 atcaatgaga ctgatccaca agcagcaact tcagaaggga acttgctggg aggagtaaaa
     7861 aatccgtttc ttgaggcaag tgaatggggc tggcagatcg atccagaagg gctgagaatc
     7921 gcattgaatg aattatacaa tcgttaccag aaaccattat tcattgttga aaacggtctt
     7981 ggagccattg atcatatgga agaggatggc acgattattg atgattatcg gatcgattat
     8041 ttacgtcgtc atatcgaagc gatggctaat gcagtagcag acggtgtaga tttgatgggc
     8101 tatacgcctt ggggatgtat cgatcttgtc agtgcatcaa caggagaaat gagtaaacgt
     8161 tacggcttta tctatgtgga tctagacgat gaaggaaatg ggacactgaa tcgatcaaag
     8221 aaaaaatctt ttgattggta taaagaagta attgcctcaa atggcgtaaa cttgtaatta
     8281 aagcagctga atgaaaaaga acacacttct cacatgtgat ttgtgttctt tttttatttt
     8341 ttatgtgcta ttcttttagt aatagtaaag caaagggtga cgttatgaaa aggacacgtg
     8401 tactatactt ggaagtcgca gataaaatta aagaagatat tttctcagga aaatatccag
     8461 tcggttcgat gttgccgaca gaaagtgaat tagaagaact gtttcaagtc agcaaaatca
     8521 ctgtccgaaa ggcaatcgaa ctgctggcat cagatgaata tgtcgcaaag aaaagtggtc
     8581 gtgggacgac ggttctaagc aatcgtcctt acaatcgatt atccaaagcg gcaagcttta
     8641 cgcaaatctt ggagaactcc cgatatgaag tccggaaaga aacgctggat ttgcaattgg
     8701 tccatataaa gaaggaagat cctttgtttt cctttttcgg tgagcaagct gtttgtttta
     8761 agcgcttata ttatttgaac aatcagcctt atatttattt tgttcattat cttccacaag
     8821 aaatgatgca attcagcaga gaagaatttg gcaaagaatc tttgtatcgt ttgctgacga
     8881 aaaaaggata tactattgac tcattttctg atgatttctc ggcagtattt ttaacagaag
     8941 aagaacaagg aattctgaaa acagacgcac agatcggcat caaacggata cggaaatcgt
     9001 ctacgcaaat tggggaaatg attgaatatt cggaggcggt ctaccatacg gagctgcatc
     9061 cttaccatat tgattatgaa acataaataa a
//
LOCUS       DBNICI010000115         9004 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.116, whole genome shotgun sequence.
ACCESSION   DBNICI010000115 DBNICI010000000
VERSION     DBNICI010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.116"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(225..1682)
                     /locus_tag="AC1ZCC_002119"
     CDS             complement(225..1682)
                     /locus_tag="AC1ZCC_002119"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HIQ1630354.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(1679..3574)
                     /locus_tag="AC1ZCC_002120"
     CDS             complement(1679..3574)
                     /locus_tag="AC1ZCC_002120"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HIQ1630355.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(3632..5407)
                     /locus_tag="AC1ZCC_002121"
     CDS             complement(3632..5407)
                     /locus_tag="AC1ZCC_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HIQ1630356.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(5457..6953)
                     /locus_tag="AC1ZCC_002122"
     CDS             complement(5457..6953)
                     /locus_tag="AC1ZCC_002122"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HIQ1630357.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(7147..8769)
                     /locus_tag="AC1ZCC_002123"
     CDS             complement(7147..8769)
                     /locus_tag="AC1ZCC_002123"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_function: GO:0004574 - oligo-1,6-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009311 - oligosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HIQ1630358.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
BASE COUNT         2666 a         1879 c         1453 g         3006 t
ORIGIN      
        1 tcgttttttg agctaatgcc gatggtcagc tgattgtaca ccgtttattg agaggtcgtg
       61 agcctgccgt tgaactccga gtcacaagga gcaatttcaa aaaacagctt ctcatgtttt
      121 atgactatgg tgacttgtga tcgaggagtt ggcaggagaa tactgtttat tcgtaaagaa
      181 ggggctgtga caagactttt gtcacagtcc cttatttcct tttattatag gatttcgcca
      241 tttgattgga tgacttgtct gtaccaatca aatgattttt tctttaaccg tttctttgtg
      301 ccattccctt cgtcatcttg atcaacatag atatatccat aacgttttga catttgtgaa
      361 gtagaagcgc tgacgatatc aatgcagccc cacgacgtat aaccaaataa atctactcca
      421 tctttgatag cttccttcat ctgctcaata tgctgcttca aatagtcaat gcggtaatcg
      481 tcttgaacgg ttccatcttc ttctactttg tctatagctc cgagtccgtt ttcagctatg
      541 aacagtggtt tttgatacct atcatataat tgattcaaag caattctcaa acccttagga
      601 tcaatctgcc agccccattc acttgatgga agatacggat ttttaattcc tccagttatt
      661 aagtttcctt ggaccccttc ccaattttct ggatgagcac tgactgcact agacatgtag
      721 tagctaaaag aaacaaaatc aacagggtag gctgctaaga tttcttcatc acctacttct
      781 atttttaagt tgacaccttt ttcttcaaac attcgttgcg tatacgctgg ataatatcct
      841 cttgcttgaa catcactaaa gaacaaagaa gttctttgct gctcaattgt tttttgaata
      901 tcatccggat cacaagaata cggaacaaga agctgatagc tgatcataca accgacttgt
      961 gaacctttga taatttcatg acaagctttt gtagctaaag cacttgctat aaactgatgg
     1021 tgtgcacctt ggtatttcgc ttgttccaaa tggggatggt tttcttcaat tactcctacg
     1081 ctaacaaaag ggtgatgttt tacacagttg atctcattga aagtcatcca atattttact
     1141 ttatttttat agcgtttgaa tactgtttca gcgaaacgaa cgtaaaactg aataagcttt
     1201 cgattaggcc atccaccata ttctgtaacc aaatataacg gcatttcata atgggaaaga
     1261 gaaacgactg gctcaatccc atattttaga cattcgtcaa acacttgatc ataaaaaaat
     1321 aatcccgctt cattgggtgt ttcttcatca ccatgaggaa aaattcttgt ccatgcgata
     1381 gacatccgat agcatttaaa acccatttca gcaaataaac ggatgtcttc tttaaagtga
     1441 tgatagaaat catttccgtg tcttttagga taataatact tgctgttggg atcctgcgcc
     1501 gcttttactt gctgatgcgt catcatatgc cattgatctg tccatttttc tttaggtaaa
     1561 tcgctgtcga atgtatacac atcagacaca gataatcctt tttcatctac atcccatgca
     1621 ccttcacatt gattggccgc tgtcgctccg ccccataaaa atccttctgg aaattttttc
     1681 atatcgatcc ccctaaactt gctagttttc ttgtttgatt atttttctta tgactagtaa
     1741 tgatattttt gaatctacta ctatttgtaa caataaccat tgtggtcgga tcatatccct
     1801 ctttgattaa ttcttgcaaa tctacttcca tcaataaatc gcctttacgt actttttttc
     1861 catcagatac tgcactttga aagtatttgc catttaaatt cactgtatca ataccgatat
     1921 ggatcagtat ctctgctcct tcttcactga ctaccccaat tgcatgtttc gttggaaaaa
     1981 ctgttgaaac ttccccgttt acagaactgt agatttttcc gtccgttgga atgatcgcaa
     2041 acccttctcc taaagatcca ctagcaaatg ttggatcgtt tacttcagat aaatcaatta
     2101 tttcacctgc tgcaggagta cttaacacta tatcctttga ggattcttct tcgatttcat
     2161 ccttgtagcc gaaaagaaaa gttagcatac aggaactgat catcgctaag aaataactag
     2221 caactagcca aacaaatcct tcaccaatat aaatcgggaa agacaaaata cttcttgtag
     2281 caactgccaa agcaccggct cctgcggcac cagctactcc accaaatata gctccacata
     2341 tacaagcaat gtaaaatggt cgtttatatt taagagtcac accataaaca gcaggttcag
     2401 tgattccaaa taatgctgtg ataaatgcag ataaagataa actttttact ttcttatttt
     2461 tcgttttcaa aaatacccca agtactgctc ctgcttgcgc agctacggca gtcatacatg
     2521 ctggacctag cgtatcacga ccgtagaaag aaagattgtt ggtcattact ggtacaagtc
     2581 cccaatgtaa cccaaaaatc actaacactt gccaaaatcc gccgataaca gcaccagata
     2641 aaacagggct aaagttataa acagctaaat atccagctgc aatcagctga cttgcataat
     2701 ctgcaattgg acctacaact aataaaataa ttgggaccat cacgatgata caaatgaatg
     2761 gtgtcaataa atttcgaata ctttcgtgca agcttttgcg aatcttaggc tcaatataag
     2821 aaagaaccca tacagctaaa attgctggaa taaccgtact tgtatacctt gctagaatca
     2881 cgggcatccc tagaaatcgc aaggttatgc tatcactata tgcactgaca atcgtaggat
     2941 aaacgagagc tgctgcaata accatagcga tgaaacgatc tgtattgaac ttttttgcag
     3001 cagtatatgc caaaaatata ggcaagaaat agaaaaatgc atcagctgct gcattcaaaa
     3061 tgatataagt tcctgattca gtagttaata aattcaaact cgtacataag gcgagtactc
     3121 ctttgatcaa accacttcct gccaagacgc ctaaagtcgg ggcaaaaaca gcggcgatca
     3181 tgtctataaa agagtttaaa atgccttttt tctcagttcc tctctttcca ctagatgcat
     3241 catcttctag tttcatttgt ttggataata cgtcatacac ttcatttact gcatttccta
     3301 ctacgacttg aaacattcct ccagcttcga ctacagtgat cacaccttct agttgctcta
     3361 ccttctcctt atctgccttc ttattatctt ttagggtaaa gcgtaacctt gttgcacaat
     3421 ggtaaacatt gctgacattt tcttcgcctc cgacaaatcg gacaatatct ttcgctaatt
     3481 gtgaatagtc catttttact tcccccttgt taattaatgc cgtagcaatc cttcttacaa
     3541 gataagtata gttcttagaa aacggtttct ccattcaatt agtacccctt ctaatagcga
     3601 acgaaactgt acaatagtgt gtatggttta cttagccgag caaatcactc actgcttgaa
     3661 agaaaacaag tatatcgttt ctcttatagt caaaggagac aaaaaaaaca cgcttgatct
     3721 tcctttgctg atgtataagt ggtatctcaa gctgatactc tgtgattttt gtttctactt
     3781 ggcttttgat aagacagcac aacaaagtct cggcatttaa cggaagcata actaggtcag
     3841 aactaggaga gattagttgc tgaatggaat ctatcttttt atggattttt atttgatttt
     3901 cttctttgta gtaatgatca agcagttttg ttttcttttt ttcttcaact atggtcagct
     3961 tttcacgcaa aatctgataa atctcccaag attcaaactc atctagaaca ctaggtagat
     4021 aaatcaaatc atcccactca aaaataagct cttgctcagt agtcaaaatt gcatcatatt
     4081 ccgttatttt gtttgattga tttttgaaaa gatacactgg ttctatccgg cattctttga
     4141 tccacatgtt catactttgt tgaatctgtt tttttagtgt attcaaaata ctaattggct
     4201 ggttactaac aatcagtatc tccccttgtt ttcgaaaagg attcaacgct tcggcaagat
     4261 acaaaacaaa ataagcgctt tcagctaaat tgggacgtaa ttgaaaatgt attgggaaga
     4321 aacgccgaat taaataaatt tctaaaggat aacgggagat gatcttttgt acaaaatgat
     4381 tcatggtatt atgtccggca tccatcctcg ttttcatttg tttgatatgg gttagcaaaa
     4441 attctgtttc atggaacaat aacgaaacag gaagatctaa aaaacgaata atcgtttgct
     4501 caaatgctct tagattcttc tctatttctt tgtttttttc atcattaatc atcaaataat
     4561 ttaactctaa tagtcgattt tcaattgctt gtttttcttg attggaaaaa gaatagggta
     4621 gttctgatga taactttttt gtgagggtct ctaccatcgg aataaataat gtatacctgt
     4681 ttgttttttc taaaagttct tctcttcgaa gaatggtcaa aacgagatac cgagaaaatc
     4741 gtatgaagtc ctctcctgaa acaatataat tatatgatat aagaacctct tttaatgtct
     4801 tttggacagt cggcatgata ttggaaaaaa ttgtactgat ttgttgagga aaccttgcgt
     4861 gttggatcac cgtttctgtt ccaacaagag ataagaaaat tctttttgaa ttttcttcag
     4921 caatgatttt tagtcctttt gtcgacgaaa cttccagttc taatttagga ttgcgctcga
     4981 tccaacgcaa aatcgttttt atttgtaatg atacggctgt ttttgaaaga aagaaaatgt
     5041 ctgctatctt ttgcatactg acataccctt tttgaaacaa taagtacgtc acaatagttg
     5101 aagctcggaa atccatgctg gaatagattt tatgtttcgt cacctcttgt atgatttgct
     5161 gtttagtttc agattcaatt gattctaaaa aataacctga acgtttattt gactggataa
     5221 tgattttctt ctcgtagata gcaagcatat ttttgatttc agtttggatc gttttcttgt
     5281 tggtacctaa agcttcagct aattcttcgc ttgttatcca atgattctta tttcttaata
     5341 ctacaagaat ttggagttgt cgtttattta agtccatttg cttacacctc ctctttgtta
     5401 taaacataca ggaaaaacct ctcaaatgac aatagtcact tgcagaggtt taaaaatcat
     5461 ttgaagaaag atctatattc aaggttttta ccattagact ggataatttg tttataatat
     5521 tcaaaactat cttttttcaa tctcctaaag cttccgctcc catcattttg tctatccaca
     5581 taaataaagc catatcgctt cctcatttcc cctgttcctg ccgatacgat atcgattggt
     5641 ccccaccata agtaaccaat gatttggcaa ccatctttta ttgcctcagc aatttcctga
     5701 agatgtattt ttagatactc acaacgtttt ggatcgtgga ttcgattcaa ttcatctggt
     5761 gcttcatcta gtccaatgcc attttcaact atgaataatg gtaattcata acggtctgtc
     5821 aattcattca tgacataacg aaaccctaga ggatcaattg gccaactcca aggttcggga
     5881 gatttttcct tcaaaaaggg attatcgaaa ccatttgctc cgccagtatc tacgcgcatt
     5941 tctgcttctt ttttatagac agcgctacga taataactaa atgcaatata atctgccgtg
     6001 ttagcttgca agcactgttc gtctccatct aacattactg gtgcacagtc ttcttccttc
     6061 catgtcctgt aaatataagc tggataatgc cctcggcaca tgacatcgga gaaaaaccat
     6121 gaattgcgtt taaattgcag tgaaccaaat acattctctg gatcacagtc atatgcataa
     6181 gtcgctaatg cagaaaagct acacataata cccatcttag catttggatc gattttcttt
     6241 aataattgag tagcacgggc gttcgctaga aacatatgat gacaggcttg ataaatcact
     6301 ttttttgtta gtgtagcaat gggttcaagt ttatctctag cagggaatgt aaccaatcca
     6361 gcagctgcat aaggcatacg aaaagcatta ttgatctcat taaaggtcat ccaatatttc
     6421 actttccctt ggtagcgttc aaagacggtc tgcgcataaa attcaaaaaa gtcgattaat
     6481 tttcgatttt gccacccccc atactctcct accaatgcta agggggtttc ataatgacta
     6541 agagtaatta ctggctcgat atcatatcga tttaatgtat tgattagtct gtcataaaat
     6601 acgagaccag cctcatttgg cttctgttcg tctcctcttg gaaatattcg tgcccatgag
     6661 atgcttgttc gaaaagcttt taaacctaac tccttcagta aacgaatatc ctcttcaaat
     6721 cgatgataaa aatcaatcgc ttcatgagaa agataatgga gtgattcgtc taaagcaatc
     6781 tcccaaatct ttttttcttc attccactga atactaggat gctggttcat gatcccgata
     6841 agcgtatctg tcacattagg taatttgccg tcttccaacc atgcaccttc acattgatta
     6901 gcagctactg ctccgcccca taaaaaattt tctggaaata tttcctgatc cataactatc
     6961 tctcctcgct atcaacaatt tcttacttga agtatagctc gattcatcaa aacaagatat
     7021 tctaactatt tcggcagaag agaagaaaaa aattgaatgc tactcttcat ttttaatatt
     7081 tatatataac aaatacataa gacgatcccc gaaagaaaac actcgttaac catctaagca
     7141 aacgatttat tttacttcca acacaaatgc ttcataaggt cgtaaatcaa ctttgccgtc
     7201 tggtaactgt ttcagaggat aatttgacac aataaccctt aaagcttcac gtggaatagt
     7261 gaacgtctgc ttttcactgg acatattaca aataacgagc cattgctggt cttcatatat
     7321 gcgagaatag gcaaagacat gctcaggcgt cttttccact aattcatagt ctccccagac
     7381 gatgatgtca tgttttctac gcaaatcgat caatttcttg tacatataaa aaacagaatc
     7441 ttgatctgct aacgcttctt ctgcattgat ttctttataa tttggattga ctcgaagcca
     7501 tggaattcca gtagtaaacc cagcatgttc acgggcgttc cattgcatag gcgtcctagc
     7561 attgtctctt cctttcgcct tgattgattc caagatttcc tcttttgtaa atccagaaga
     7621 aatcctttcg ttatacatat tgattgtctc aatatctttt gcttccgcga tatcatcgat
     7681 tgggcaattc gtcatgccta actcttctcc ttgatagata taaggggtgc ctttcatcat
     7741 atggagcaag atagcaaaca tcttggcact ttcttttcga tacacatcgt catttcccca
     7801 acgagaaaca attcgcggaa gatcgtgatt attccaaaat aaactattcc atccttcatc
     7861 acctaaagat gtttgccatt tggataatac ttttttcagt tcagagatac tcaagggttt
     7921 caaatcccat ttgtctttgc cttcttgctg atctaatcct atgtgttcga attggaagac
     7981 catggataat tcatttcttt taggattgga ataaagttta gcgatttcgg tagttgctcc
     8041 ccatgtttcg ccaacagtca tcagattttt gtcaccaaaa gttgcttgat tcatttcctg
     8101 aagatactca tgcagtttag gtccattact tgtgatttct ttatccggtt ctttgccaat
     8161 catatcgatc acgtccatac gaaagccccc aataccttta tcgatccaga aattcatcat
     8221 ttgataaatc tcttggcgaa cctgtttatt tttccaattc aaatcaggtt gacgcttgct
     8281 atacaagtgc agaaaatatt gccccgtttt ttcatccaac tgccaagcag aaccactgaa
     8341 tattgagcgc aatctattag gctcttttcc attgaccgga tcacgccaaa tataatagtc
     8401 ccgataagga ttttctttat ttttacgggc ctcaatgaac cacggatgtt catcagaagt
     8461 atgattcacc accagatcca tgatgatacg gatatttctt ttctttgctt cttcaatcag
     8521 ttcttccatt tcttccatcg taccgaattc tgccatgatt gcttgataat tactgatatc
     8581 atacccattg tcatcattcg gagactcgta tactggagaa agccaaattg catcaatacc
     8641 taaatacgcc agataatcca acctggaaat aatcccccgc aaatctccaa taccatcttg
     8701 atttgaatct tgaaaacttc ttggatagat ttgataaaca actgaatggt gccaccattt
     8761 ttgttccatg attttcctcc tataaatgga tgtttctatt ttttgcttaa tcatttattt
     8821 tagtctctct cactaaaaat tgtaacgctt tcaaatagct attgcacctt gttatcggta
     8881 acagaataaa acaaaaaaac agctgaggaa attccctcaa ctgttttttg aaaggattga
     8941 tttatgcagg gaagaatgct gatttcacaa cttctttata aacggtgttc actcagctgt
     9001 ccct
//
LOCUS       DBNICI010000116         8767 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.117, whole genome shotgun sequence.
ACCESSION   DBNICI010000116 DBNICI010000000
VERSION     DBNICI010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.117"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            127..3654
                     /locus_tag="AC1ZCC_002124"
     CDS             127..3654
                     /locus_tag="AC1ZCC_002124"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0032508 - obsolete DNA duplex unwinding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIQ1630359.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="AC1ZCC_002125"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="AC1ZCC_002125"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HIQ1630360.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTDAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="AC1ZCC_002126"
     CDS             7442..7783
                     /locus_tag="AC1ZCC_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIQ1630361.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     misc_binding    7827..8038
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8092..>8767
                     /locus_tag="AC1ZCC_002127"
     CDS             8092..>8767
                     /locus_tag="AC1ZCC_002127"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIQ1630362.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNI"
BASE COUNT         3048 a         1501 c         1861 g         2357 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgatgc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatca
//
LOCUS       DBNICI010000117         8636 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.118, whole genome shotgun sequence.
ACCESSION   DBNICI010000117 DBNICI010000000
VERSION     DBNICI010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.118"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..946)
                     /locus_tag="AC1ZCC_002128"
     CDS             complement(<1..946)
                     /locus_tag="AC1ZCC_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HIQ1630363.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQK"
     gene            1050..1652
                     /locus_tag="AC1ZCC_002129"
     CDS             1050..1652
                     /locus_tag="AC1ZCC_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HIQ1630364.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(1740..3239)
                     /locus_tag="AC1ZCC_002130"
     CDS             complement(1740..3239)
                     /locus_tag="AC1ZCC_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0016600 - flotillin complex [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HIQ1630365.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3255..3779)
                     /locus_tag="AC1ZCC_002131"
     CDS             complement(3255..3779)
                     /locus_tag="AC1ZCC_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIQ1630366.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            4077..4826
                     /locus_tag="AC1ZCC_002132"
     CDS             4077..4826
                     /locus_tag="AC1ZCC_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HIQ1630367.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4817..5602
                     /locus_tag="AC1ZCC_002133"
     CDS             4817..5602
                     /locus_tag="AC1ZCC_002133"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIQ1630368.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            5571..6287
                     /locus_tag="AC1ZCC_002134"
     CDS             5571..6287
                     /locus_tag="AC1ZCC_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIQ1630369.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(6389..7408)
                     /locus_tag="AC1ZCC_002135"
     CDS             complement(6389..7408)
                     /locus_tag="AC1ZCC_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIQ1630370.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYYIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7427..8428)
                     /locus_tag="AC1ZCC_002136"
     CDS             complement(7427..8428)
                     /locus_tag="AC1ZCC_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIQ1630371.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         2597 a         1748 c         1536 g         2755 t
ORIGIN      
        1 ttttttgttg ggagaattct gccttgattt caataaaact tttaaaagac tgcaaaaact
       61 ggaacaattc tgctcgatcc tcgaattcag ggcgactagt cggcagaggc atttgccgat
      121 acaatacata aacttcttca atacgtgtta aaatttgtct gccatcattt gactctgaaa
      181 atgtttctgc agtataaatc aataaaccat agatatgttt tccataataa gctggttgct
      241 ggattctctc tagattttcc agcatgtctt taatcacatt ggcctgtgct cttctcatag
      301 agaagtaagt ttcgtagtat agcggctgtc tgagccagta gttttcttga tgttctctgg
      361 catcttcttg gctttcgcga atgaatgtca gtaaatgttc gcatttgatc tgcacatctt
      421 ctttattttc tgataaaaga aattctgcca tttgtctcaa aatatttcga aattcttttt
      481 cgatctgtat ttggttttcc ttcagctgct tcgtacgatc tggcatgtaa acatttgcaa
      541 gcaaagcaaa tactaatcca atcgccatta atcccgcttc attgccgatc agctgccaag
      601 acatgttttc ctccactaaa taatgagtga ccaaaaccga atttactact atcccttcgg
      661 ttaatttgaa gcgagaggca agcgggatga acaggaataa aaaaagactg aagccaaaaa
      721 tagtataccc aacagcaaaa aaactgaaca gtgctgttgc cgtagctaat ataaaggctg
      781 cgattcgatt catgcccgta cgcaatgtag agcgcttggt atttcctaca cttaataatg
      841 caatgatccc tgcagctggc caatacaaca gcgataagct acccgctgtg accatcgaaa
      901 agaagacact gatcactgtt ttgactgttc gcattcctat acgcatttac ttcccccttt
      961 tttattttaa ttatacttga ataatgaaaa actagctatc agacaaaagt ttaggtatga
     1021 tagtaaaaag accaaaaagg agaatctcta tgcaatttgg ccgttttcga ctaggtatgc
     1081 gtacgatcaa aagtgcctta gctgtttttt tatgtatctt gttttttcat ttgactcaac
     1141 gaggaatccc gatgattgct gcattatctg ctgttttttc tttgcgtcag gacttgacta
     1201 caaccgtttc ttttggaaag tctaggatca tcggcaatac attaggtggt acattagcta
     1261 tcatttattt ttttgtcaaa agctattttc acaatgattt tttggtagaa ttgatcttgc
     1321 ttcctattct agttattatt gtgatcgtcg tgtcagatgg tattaacaat aattccggta
     1381 tcatttccgc aatagctacg ttgttgctga ttgcgttgag catccctcaa ggcgaatccg
     1441 ctttgtatgc tatccaacgt gtactagata cttttatagg gacattcatt gcaatcggta
     1501 tcaatttctt tcttcgtcct cctgaaacag aaaagaagga agaactatca gaagatttgg
     1561 tcgaactcca aaagaaagaa cgattgctaa gaacaaattt acaagagata gaagctaaga
     1621 tccaaaaaca aaagaaaaat agcgaggact aattttcttt tgcaataaaa taaaaaaatg
     1681 gagagaagaa ctttaaaaag tcttcctcca tttttgattg aacggtgaag tgtactactt
     1741 tattcttcct tcatcgagtt ttgttctgcc tcttttggcg cttcatacat atttacttgg
     1801 ctagtagtcc cacgtgtcga caaattctct acgatttcct tcaagtcaag tcctgttgtc
     1861 gcttttagtg tttcttgagt agaagaaagc aaatttgtgg cataatttgt cactcggttc
     1921 gctccactgg tttctcctga actgcttcct gtatcgacaa cagagatttt ttcgatattt
     1981 cctagcggct gtgctgcttc gcgcatcaat tgcggtagca tatcgataac catactcaag
     2041 acagctgctt cgccatattc cttgaaggca ttggcgatct tttgttttgc ttcggcttct
     2101 gcttttcctt ttgctagtgc tgcttcggct tctgcttgtc cagctaaacg tgtcttattg
     2161 gcttccgctt ctgccagtgc ttccacacgg aatcgttctg cttcggcttc agtcacttct
     2221 ttgacttttt gagcttgtgc ttcttgttct ttagcgtacc gatcggcatc tgcttttttc
     2281 ttcacttccg aatcatattg cttttcacga cgtgtgattt ctttttcttc cagttcgatt
     2341 tgtttttgac gttctacgat tttaatctgc atttcctgtt cgattacttg ttgttgtgct
     2401 cgggcacttt ctaaattgta ggcttggtct gcttttgctt tagcaacatc ttgttcttgt
     2461 ttgtaggttg ctaatttcag ttctttttct tttaatgatt ctgcgatctc tgtttgtcgc
     2521 tgtaattcag ctgcttgtga ttctttttct gcttgagctt ttttgatacg cgtttctttt
     2581 agagcttccg cttctgctat atctgcatct cttttgactt gggcgattct tggtttccct
     2641 aaagagtcaa gataaccgtt tttgtctctg acctctttga tagtgaaaga aacgatgatc
     2701 aaccccattt ttgcaaggtc tacagaagcg acttcttgta cgctttgact gaacttatcg
     2761 cgattttgat agatttcttc tacagtcata gaaccaagaa tcgagcgcaa gtgtccttct
     2821 aatacttctc ttgcttcatt ttccagttct tctcttgtct tgcctaaaaa ctgttctgct
     2881 gctgtagcaa tctcttctac agatgaaccg attttgatga tcgaagtacc atcacacatc
     2941 accggtacgc cttgttccgt atacacttca ggggtggaga cgtccaattt actcgataat
     3001 agactgatcc gattcgaacg ttgaaaaact ggcaagacga atgcgccacc tccacgcaca
     3061 attttgattt tgttattgct ttcatcaatg tgcacatttt tgcttcctaa ataacttcca
     3121 ctgatgatca acgcttcatc cggttttgct gtctggtact tcgctacaaa gacgatcagc
     3181 aacatcagta aaatcaaagc tgcgattgcg atcgcgataa aaacaactgg tatttccatt
     3241 taacacacgc tcctttaagc ttttatcatg ctttcataag gcatgacata acagattctt
     3301 tccttgatct caatgatcaa tactttcgtt ccccgctcag ctgtctgttc ttgggggctg
     3361 taaaaacaag ctggacgact aatggaaccg gtaacacttt tcaactgaat ttctcccata
     3421 cctcgtacag ggatcggtgt gatcacaaca gctacttgtc cttccagagt tttttcagaa
     3481 gacgacatcg ttgattcggc atgtttcatc ggcatcaata tatagaaatt aagcaaaaat
     3541 accaacacta tcgatatgcc tccactagca ataaataaaa ataaagatga ttgatctgtt
     3601 aaacgctcac ctagaaaacc aaataagctt gtgaaagtta accaagggat aataagcatc
     3661 ggatcaagcg ggccatcaaa atcgaataca tctccaaaaa taatcaatag gactgccaat
     3721 cctgcacaaa tcagcaatgt ataccaataa atcgtctcaa gtgcgattcc ccctatcata
     3781 tacacgcctc cttccatttc tacgtctatc ataccaatgt ttgaaaattc tgtatagctt
     3841 cttttaccga ctcagtggtg ttttttatag gaaggttgtg tttatcgttg acaatttgtt
     3901 aaatttttgt tttcatgtta aaattttcac aataaattct ttcgaggtag cctatgtttc
     3961 ctttttttaa gaaaaaacca ttaaagctcc agaacacgat catccttgtc gtttgtttct
     4021 ccacattcgt ttctttgatt gtttctgcca tattgatcag gaattttatt ctttcgatga
     4081 accgatctag tgtgtcagat aaaatctcta gcattgctaa aatcgttgcc aatgataaac
     4141 tcatccaaaa aaagctgcag actggtgacc cgacacatgc gattcaagga ataacaaatg
     4201 aaatcatgat tgcatcgaat ttagatttta tcgttgtcat ggaccctgaa ggcattcgat
     4261 attctcacgc agacccaagt tacatcggtc ttcctttctc aagtcatgaa gatgtgaaaa
     4321 aagtgattgg aggacaagaa tttttctctg aaaaagaagg aatgttaggg ttcggcttcc
     4381 gctatttcgt ccctatttat tatgatcaag agattattgg tgcagtttgt gttggcttaa
     4441 caaacaacac gattgacaat caagtcaaag atggacaaaa aatgattgtt ttttcattga
     4501 ttattggact tagcgtaggc atcataggcg ccgtttttct tgcccaaaaa atgaaaaaag
     4561 tactgcttgg acttgaacca caagaaatag cagaacagtt aagtgaaaaa acgattatcg
     4621 aaaatgaagt agatgaagga atcttagcca tctcttctga taaaactatc ctcttgatca
     4681 atacacctgc aaaaaacatt ttctaccaaa cagagcagct attggatttc gaagtaggaa
     4741 aaaaatcagt gataatctat atgacgtttt gtttaaagag acgattgagc atcaaacgac
     4801 cgttcggact caatccatgt atttaaatgg gatagaaact atttctagcg ttacacctct
     4861 ttttagcaat gaccgttttt taggagcagt cgcaacttta cgaaatcgat cagaagtaat
     4921 gaaattaatg gaggagcttg gggggacgaa acaatatata aactccatga gagctcaaac
     4981 ccatgaattc atgaataaac ttcatgtcat ttctggtttg attgatttga agaagtatga
     5041 agaagtcacc cattttatcc agcagctaaa tcaaaattat aaaaaagaga ttggccaagt
     5101 ctcaagtctg atcaaagtac ctgcaattgc cggtttttta ttaggcaaaa taaacgaagc
     5161 caaagaacaa tctgttgaaa tgctgttaga aagcgattct tttatcccta aaatggagat
     5221 ggatgactcc atccattcac ttttgcaagt gttagggaac ttacttgata atgcaaaaga
     5281 agccgtgcta caaaaacatg aaagaaacgg acaggtccgg attcatttaa actatgacac
     5341 agaagctgaa gtattcatcg tgaccgtctg cgatgacggt gtcgggatat cagaagaaat
     5401 ctcagaaaaa atatttacgc taggtttttc gacgaaggga atcaatcgtg ggtttggatt
     5461 gaatctcatc cattcaatcg tcaaacgcca cggtggattt ttagaatttt ccaaacaaac
     5521 aaatggcggt acaatcgtct atctagaatt accaaaaaag gaagatcaaa atgatgacaa
     5581 atgtcctgat aatcgaagat gatccaatgg ttgcagaatt gaatcgtact tttgtggaaa
     5641 gcatccctaa tatgaacgta atcgaaagtt tcagaaatgt agaagaagga aaaaaatatt
     5701 tcagacaaaa aaacgcagat ctgctgctgc ttgatgtata catgcctggc gaaagtggta
     5761 tccatttttt atcttggctt agacagcaag gaaatgatat cccagtgatt ttgatcactg
     5821 ctgcagatga tatcgaaacc ataaagaatg tgatccgcta cggtgcctta gattatttga
     5881 tcaaaccgtt cactttgcaa cgttttcagc tggcgatcga aaagtatctt gctttctctc
     5941 agacaatcca aacgactgaa aaagccgatc agaaattgat cgatcaagta ttcaaccaca
     6001 ccactcccat tcacgaaata ccatcattca tcgatttgcc taaaggtctg tcaaaactga
     6061 ctttaaaaaa aataagtgca tttattctaa aagaagatac tattttttca acaaaagaac
     6121 tggctcaaaa aagctcactg tcacgtatat ccgtcaagaa atatttgaat tttttagtag
     6181 aacaagatat attgaacgaa agaatcaaat atttggaaat cggccggccg ctaacctgtt
     6241 atgaattgac aaagaatggg cggaaaaagc tcacagatta ctgttgagtc atcgaattat
     6301 cccatacaaa atagtccaag tatacagaag taacaactgt ttacttggac tatttttaaa
     6361 cattttattg acgaatatcc atgttttctt atcttaattg acgggcttta ggaagagata
     6421 cctcattttt cgtttttcca gtttctatta tttcacgaaa gttctcgaaa cttgtcgaaa
     6481 tcatatttga taatgcctca tctgtatttg aacctacatg cggggttaca agaactctag
     6541 ggtacatttc tactaattgc tggatagcag ggtctggaat cgtctcccat ggcttgaagg
     6601 atcggaaaaa aacctctgtt tcatttgcaa aaacatcggt tgcaaaacca gctaaatgat
     6661 tgcttttcaa agctcttaaa atagcttcat tatcttgaag ctctccacgg gctgtattaa
     6721 tcaagatgct accttttttc atttgagcta aaaatccttc attgatcatt tgatcgtttt
     6781 cacctgggat atgaggtaca tgaatactga caatatcgct tttggctaat aactcttcaa
     6841 gcgagtggaa cgtcaatact tttttagcag cttctgtctg atgaatatag tagccgatca
     6901 ctttggctcc tagtccctta aacaaaacag cttccgtcaa ccctatcctg cctgttccta
     6961 tgatccctac tgtgcaattt ctgatttcac gactaaacat ttgctcatcc actcgaaaat
     7021 ctttgaaaga tgttcggatc gtcgtatacg cagtatttct caaaagcatc atggcaaaag
     7081 tcaaagataa ctctgctatc gcatttggcg aatacgatgg tacacgggct actgtcatac
     7141 caaaatcagc tgccgcttgt agatcgatat gattgactcc cacagtccgt gtaaaaacat
     7201 aacgaatccc atattcctgc atttttgcaa gattggtctc atctgctata caatttcccc
     7261 tcaacaatac agcatcatgg tcctttgctg acacgatatt gtcatgtgtc aacagttctt
     7321 cataaagact gagatcataa tggtaaatat tcaatttttc aaaaaaatct atctcgttcg
     7381 gacgaacacc gtaacatgcg attctcatac tttcttcctc catttcttat gaaaataaac
     7441 ctatgccatg aatcatttgt aatacaacga tccatacagg tgtcagtacc acagcaaaaa
     7501 tagttgacag taaggatgca ttggaggcta aaagtgcctc cctatcaaaa ctgatagcat
     7561 aggctgctgc cactgttgca gctggtgtag ccatcatgat aacgattgtt gccagtgctt
     7621 caaaattgac tggtagaata ttcgttgccg tcaaaactgc tagaatcacg atattcaatg
     7681 caggtacaaa taccactttg actagactgt aataccatga agttttttca gatgcagctt
     7741 ttttgaagct tacttcacct aatgttgcgc cgattgctag ccacgccaat ggtgaactta
     7801 aaccagctaa ataatccatc ggctgaaata gccacggtgc agtctggtca attcgcaaaa
     7861 acgctacttg gcttatctct ccttctgcac ctgtaactga cacttgtggc agataccctt
     7921 ggaacagcca aatgaaaagg ccggcaaaag tagcgataat gattggattg acgaacatcg
     7981 ctttgatatt tttagcttcc atcttcaagc cactcatttt tatgtatcca taagaataca
     8041 aaaagattcg gtagccaata ttgaaaattg aggaatacat cacgccgaca ggaccataaa
     8101 tcgcactgac gataggcgtt ccgaaaaagg tcgtagaacc aaagatcgtc aggacacgca
     8161 acgttgtttg cttatcccct ttatagctta agaaaaaagg ttttgaaact agaataagaa
     8221 taatataaat aactaatccc caaatcaaga cattgattcc ttgcatcagt aattctgtat
     8281 tgatatcttg catgaaagct cgaaatgcca gtgctggcaa agccactgtt aagacgactt
     8341 tcgatagaat ttttccaaca gattcagaaa aaatcccctt ttttctacaa acaaacccta
     8401 ataatataat aaaaactgtt gaagtgattg cactgacaat cgacatatct gtcaaagtcg
     8461 atcgaaaaat gtcaaataag ttcatctttc tcctcctaat tatgttcatt tattcacaat
     8521 aaaaaaagct tttattttgc taatacgtat taacattttt aattttatct ttacattgtt
     8581 acttattgaa caataggaaa ttaaagaaac ttttgaaact tatgtaaagt gatttt
//
LOCUS       DBNICI010000118         8406 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.119, whole genome shotgun sequence.
ACCESSION   DBNICI010000118 DBNICI010000000
VERSION     DBNICI010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.119"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(98..1066)
                     /locus_tag="AC1ZCC_002137"
     CDS             complement(98..1066)
                     /locus_tag="AC1ZCC_002137"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIQ1630372.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            1298..2131
                     /locus_tag="AC1ZCC_002138"
     CDS             1298..2131
                     /locus_tag="AC1ZCC_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HIQ1630373.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            2165..3004
                     /locus_tag="AC1ZCC_002139"
     CDS             2165..3004
                     /locus_tag="AC1ZCC_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIQ1630374.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            3030..3590
                     /locus_tag="AC1ZCC_002140"
     CDS             3030..3590
                     /locus_tag="AC1ZCC_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIQ1630375.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            3789..5510
                     /locus_tag="AC1ZCC_002141"
     CDS             3789..5510
                     /locus_tag="AC1ZCC_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HIQ1630376.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            5675..6817
                     /locus_tag="AC1ZCC_002142"
     CDS             5675..6817
                     /locus_tag="AC1ZCC_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIQ1630377.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            6833..8044
                     /gene="thiI"
                     /locus_tag="AC1ZCC_002143"
     CDS             6833..8044
                     /gene="thiI"
                     /locus_tag="AC1ZCC_002143"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HIQ1630378.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         2881 a         1454 c         1743 g         2328 t
ORIGIN      
        1 ttgtttctca aatttttgta aattttcgac ttagttttcg aagaagctat ttttgtcaca
       61 gcctctcttt ttaagtttag ctattagata atgatgttta gttttggata aacgaaatca
      121 attcttcacc cgacaaacca tagtttacag caatcaatag ttttaaacgt gctttttgac
      181 tattaatact gttacagaaa ataatcccca ttttttctag ttcttttccc ccgccttcat
      241 aatcataaac aggctctgcg attccattga aacaacgaga aacaagtact acagggattt
      301 tttggacaag aagcttttcc aaagcagcta aggcttgtgg agggagattc cccgcaccta
      361 aagcttcgat taccaaaccg tctactttcg tatgggcgat ggcttccaat aagtcccctt
      421 gcatcccagc atatgcttta acgatcggga tagtcccatc tacagcggaa atgtccagtc
      481 gctttgtttc taaaagttcc tgcaagaata aaattcgatt cttagttact aagccgatgg
      541 gacccaatgt aggtgttcgg aaagtagcaa catttgtggt atgggtcttt gtcacgtagc
      601 gtgcagaatg gatctcatcg ttcatgacca ccaaaacccc cttatctagc gcttcttcac
      661 aacttgcgac acgaatcgca ctttcaaaat tataaaggcc gtcacttcca agttcattac
      721 ttgaacgcat tgccccagtc aaaacaattg gtaatttgtt gccaatcgtt gtatcaagaa
      781 aaaaagcagt ttcttctaat gtatctgtac cgtgtgtgat caccactccg gatatacctg
      841 atgcataagc ggacaagata cgttctttta ataccaacat gtgagaaggc atgatgtgtg
      901 gtgagggaag attgaagata tcttcaacta ataattctac atgcttgggg attgtgattt
      961 ctgcatctaa taatgggttt gccacatcag gactaacacc gcccgtgagc atgttttctt
     1021 tcatggcgat cgttccgccg gtgtgtagta caagtatttt tttcatttta cagacttcct
     1081 tacgaataat tttgcatagc cgttaaatct tattgtatac tatataaaag agttttgcta
     1141 tcagtgaaga ataacgaata gcctaactta tcataaaaaa gctgacctta ttaaaatgga
     1201 aaagactcaa ccttgacctc ctaatacaat ggagtatctg gttgagtttc ttttacaaga
     1261 aagcagccaa ggaaatcaat cacgaaggtg gccggaaatg atcagaatcg gattaacatc
     1321 gtttaaagaa catgacaaat taactggtaa aaagaataac actctttatg aatatgcagg
     1381 gtttctaccc ttagtggaat tagatactgc ctattatgga attccccgaa agtcctcagt
     1441 tgagaattgg ataagtgaag tacctgaatc atttcgcttt gtagtcaaag tctatcaagg
     1501 aatcagcggt caaggagact ggaagcctta ttatcagacc gaagaagaga tgattgcgca
     1561 ttttttagat gttatgtcgc caatggtaga tgctggaaag atgttttgct ttttagtcca
     1621 attcccctca tcattcaaat gtacgaaaga aaatgtccag tacttgcgca aacttagaaa
     1681 gtggtttgag ggttatcctg tttctataga attgcgggat tattcttggt acagcgatga
     1741 atttcttgaa cgtacaagaa actttatgtc tagagccgct ttttctttag ccatcatcga
     1801 tgagccacag ttgctgaata caacgattcc ttttgatcca tttgtcacca atccgaactt
     1861 tgctcttttt cgattccatg gtaggaatgc gcaaggatgg caagcaaatg ataaagagtg
     1921 gcgtaaaaaa agaacgttat atcgttattc agaaaaagaa ctgcaagaac tagcacatga
     1981 agtcaaagta gttgattcta agacaaaaga ggtaggtgtg atttttaata ataattctgg
     2041 tggtgatgcg gctgaaaatg cattagcact gcaaaaaata ttaaatatca agccagatgg
     2101 actcaatcca acccaaatgg atctgtttta agggcttatt ttattcaaga aaggggcgaa
     2161 gtctatgatc aaagctattt tttttgatat tgacggaaca ttggtcacca atcgttcaaa
     2221 agcgttggaa tcaacaaaac aagcaattga atatgctcgg aaaaacggca ttttatgcgg
     2281 tgtagcaacg gggcgcagcc cagtaaagat caaagaaatc attgatgaat tggaattaga
     2341 tatgtatgtc gtgtataatg gtcaactggt ttttactgca gatcgcacga ttatcgacca
     2401 cccttttgaa caaaaagtat tggaacatat tgttgaattt gcagatgaga accatcgaca
     2461 aatcgttttt ggtgcgagaa atcggttaga tggatcaacg acgatgctat taggacaatc
     2521 gatttttatc aagcgactag tcagcttttt accgcgtaaa ttcccggtac gtctgatgaa
     2581 aaaaatcctc caggttttta gccctcaccg acaaaaggat cgttatgaaa cgttagagat
     2641 attaaaagaa ccaatttatc aatgtatatt gctcagtccg gagtcagaac agaaaaagtt
     2701 agaacatcgc tttcccgagt gtacgtttca gcgttcaaat acgtatactg tagatatcat
     2761 cccaaaaggt ggttcaaaat tactaggcat ccaagctttc gcaaatgctg tcgaaattga
     2821 aatggaagaa attatggcat tcggcgatca ttacaatgat atcgagatgc tgaaaggtgt
     2881 aggaattgga gtggccatgg ggaatgctca gatcgaagta aaacatgctg cagactttgt
     2941 gactcaatca aatgaagaag atgggatata cgaaggactg aaacacttcg gtctgattta
     3001 ttagtcaatg aaagaaggaa aaaatcagta tggaagcaga gacacctttt caaaaagtag
     3061 aaacattcca caaaactttt gatccaagaa gaccagagat acccacggct tttacagaaa
     3121 atgaagctca tacacgtgct gcgttcaaaa tagaagaact tgttgaattt gttgcagcta
     3181 cttcacctaa taaagaacaa ctgcatgaag ctgttatgct gctgcacgaa gcattagata
     3241 aagcagaaga aaaaatactt ggtaaaacac agtggggaag tgatccgctg gttgaacaag
     3301 tagatgcact gacagatatc ctttacttta cttatggttc tttttcatta ctaggaatag
     3361 tgcctgatcg aatattcgaa atcgtccatc aggcaaatat ggggaaatta tttccagatg
     3421 gaaaacctca ttatgaccct gtaacaaata aagtgttgaa acctgaaaac tggcagatgg
     3481 aatatgctcc agaacctaaa atcgcggcag aaatccaacg tcagattcaa gaagcgattg
     3541 ctaagaagaa atccgctgat ataggcatgt gcacaaatga aaaagagtga tcagagtttc
     3601 actatttttg ttatctttat acgatggaaa aacaaaagat tttcgtttaa gttagtgaat
     3661 gcatgaaaat agtatgctat aatctagaat agatgtcttt tgcacgagag acgtattatt
     3721 tggatttatg ccaagcagaa cgccatctgt tgcataaaag atgcatactg aaaacggagg
     3781 aaccataaat gggaaataat gttatcatcg gaatcgtcat tgcaatcatt atagtcgcca
     3841 ttgtcgtctt tttggttagt tacttgatga agaaaaaaaa ccaagatcga ttggatgcct
     3901 tagaaaaacg caaggaagaa ttatttgact tgccagtgat tgaagaagtc gatgatgtta
     3961 aaaaaatgca tctggttggg caaagccaaa atacctttcg tgaatggagt caaaaatgga
     4021 atgacatatc tacttcctcg ttcgctgaac tagaaagtca aattttcgaa gtggaaaacc
     4081 tcaatgaaag ttttcggttc ttaaaaggaa aaaaagcagt tgttgaagca gaagctacaa
     4141 tggaccaaat ggagaaacaa gtgacggaaa tccgtgaagg tctaaaggaa ttgcgggaaa
     4201 cggaagaacg caactcacta gctgttcagc aggcacttga cgtctatgaa gaactgaaag
     4261 aaaaagtcag agaagacgag catgaatacg gaccagctct ttttgaattg caaaaacaga
     4321 tcaaagtgat cgaaaaagaa tttacgcagt ttgtgacttt aaacacttct ggagaccctg
     4381 ttgaagcccg cgaggttttg gataaagcag aaaaacatac gtatgaagtg gaagctctga
     4441 tgaaaaaaat tccaccgctg tatgaggagc tccataccac tttccctgaa caacttgaag
     4501 aaatcagtga tacttacgag aaattgatgg aagaacaata tgtattccca gaagaagatc
     4561 ttgctgaaga tatcgcaaaa gtaagtcgaa gaattgaaaa ttcattagcc aatcttgaaa
     4621 aaacagaagt tgaaacggtc gaatttgaaa accgtgaaac agctgatttg attgattcgt
     4681 tatatgatat cttagaacgg gaaatggaag ctcagagata tgtaaaaact aaccaatcaa
     4741 cgattgcaga gtatatcaaa catacaacaa agaataaccg tcaattattg attgagctgg
     4801 atcacacatc tcaaagctat acattgaacc acaatgaaat tggccgtgta cgaggtttcc
     4861 aaaccgaagt ggaagaaatg gaacgtcaaa atgaacagat gattccacag atccgtcaac
     4921 acgaaattcc ttattcagaa gtgcgcacgt tctataaaac agtcttcaaa gtcttagaag
     4981 atatcgaaaa gcaacaagta gagatcgacg actcattaca tgaattgaga aaaggcgaaa
     5041 aagaagcgca agaaaagatc gatacattcg aattcaaatt gcgcagtttg aaacgttttg
     5101 tagaaaaaca acgtttgcct ggcttgccga atgattacct agaattcttt tttgttgcta
     5161 ctgaccgtat tgaagaacta agtgttgtct tgaataaaat tcgagtaaac atggaagagg
     5221 tcaatcgttt agttgctcta tgtgaagaag atctagaatt attagataag aagacacatg
     5281 atttagtaga tgcagcagca ttaacggagc aaatgctcca atatgcaaat cgttatcgtc
     5341 atacacacga agaagtgaga gaagcaattg ataaaagcta ctatttattc aataaagaat
     5401 atcactatca agaagcatta gacgaaatcg gtacagcatt agagcgagta gaacctggtg
     5461 ctttcaaacg catcgaagat ttttatttta atcatccaga cttagtataa agcaaaaaag
     5521 ttagtaaacc agcaagagca agacggatta ttgtcttgtt cttgtctttt tttgtatatt
     5581 tgaaaattga aacaagtgaa aatttcacct gctcttgaaa gcaccttgga caaccgttat
     5641 aatagaaaag aatttcaaaa aagaggggtt tcttatgata tattttgata acagtgcaac
     5701 aaccccaatc tttccgcaaa gcttagatgc atatgtaaaa accagtcagc ggattattgg
     5761 gaatccatca agcttgcatg atttaggcaa tcaagctaac cggttgttgc agcaagcaag
     5821 aaagcagatc gctgatttga tccatgtaga accagaagaa atctatttta cttcaggcgg
     5881 aacagaagga gataactggg tattaaaagg aacgatgatc gaaaaaagag aatatgggaa
     5941 tcatatgatc atttcaggag tcgaacatcc ggccgtcagc gagacagcag aacaattaaa
     6001 agaattagga atcgaagtaa gtatcgcccc agtggataaa cgcggatttg tacgtgtcga
     6061 cgaattgaaa gaattaataa gaaaagagac agtattagtc tctgttatgg ctgtgaataa
     6121 tgaagtagga tcgattcagc ccatccagga aatcagtgat cttttggatg attttcctaa
     6181 aattcatttc catgtagatg ctgttcaggc gattggaaaa gtaccattct cgcaatggtt
     6241 gacagaaaga gttgattttg caacattttc agctcataaa ttccatggcc cacggggaac
     6301 tggtttcatt tattggaaaa agggtcgtcg tttagcacca ttactgacag gtggagggca
     6361 agaaagaaat cagcgaagtg ggacagaaaa tgttccaggt attgtggcta tggttaaagc
     6421 tttacgttta tcattagaga taaaggaaca aaaacctgag cagacacaac gcttaaaagt
     6481 agctctaatc aatgcgttga agacgtatga gaaagtgacg attttttcag aaggaactga
     6541 ctatgcaccg catattttat gttttgctct aaaaggaatt cgcggcgaag ttctcgtcca
     6601 tgccttagaa gaaaaacaaa tatttgtttc tactactagt gcctgctcct cacgtaaaca
     6661 tatggcaagc agtacattgc acgcaatgca tgtaccaaat gatctggcta catctgctgt
     6721 tcgaatcagc ttagatgaaa acaattcgct tgcagaagtc gagcaattta tgattatttt
     6781 caagcagctt tataagaaat tttctgcaat caattaaaag aggagtgttc ttgtgaaata
     6841 cagtgaaatt atggtccgtt atggggaact ttcgacaaaa ggaaagaatc gaaaaagctt
     6901 tatcatgcaa ctagcacaaa atgtcagaca agctttgaaa gactttccag aaatcaaaat
     6961 ccatgctgat cgggaccgga tgcatctttt attgaatggc gcagacgggc agctagttat
     7021 tccaaaacta gcaaaaatat ttgggatcca aaatttctct ccaagtattc gtgtagaaaa
     7081 agatgtcgaa gttttgaaac aagctgttca agacatcatg aaagaaattt atactgggaa
     7141 agaaacattt aaaattatcg ctaaaagaag cgatcaccaa tttgaattga caagtaatga
     7201 attgaatcaa acattaggta atgctgtttt tgatattttc ccgcatatcc aagttcaaat
     7261 gcgtcaacca gatattccat tacgtgtaga gattcggcgt gatggcgctt atctatcgta
     7321 tgaaacgatc aagggagctg gaggattgcc cgtaggaaca agcggaaaag gaatgcttat
     7381 gctttctggg gggattgatt ctcctgtagc agggtacttt gccatgaagc ggggagtgga
     7441 aattgaagct gttcattttg ctagtccgcc gtatacaagt gaacaagcac tgcaaaaagc
     7501 aaaagatcta acagccaaac tagcacctta tgtgggaacg atccatttta tcgaagtgcc
     7561 atttaccgag attcaagaag aaatcaaaaa gtcagtgcca caaggatatt ggatgacaat
     7621 tactcgtcga atgatgttgc gtttgaccga tgaaattcgc cgtattcgtc atggtttagt
     7681 cattttgaat ggtgaatcat taggtcaagt ggcttcacaa actttgcaaa gtatggtggc
     7741 tatcaatgaa gtaacgaata cgccaatcat tcggcctgta gcgacaatgg ataaacttga
     7801 aatcattgaa gtagcagaac agatcgatac atttaatctt tctatccagc cttttgaaga
     7861 ttgctgtacg atttttgctc ctccacagcc aaaaacacgc cctcaactgg aaaaggtcca
     7921 tcaatatgaa gagcgtttag caatagatga aatgatccaa cgagcactag ctggcttgaa
     7981 gatggaagag atacaagcag aatcgtctaa tgaaaaaact gaagaatttt cagaattgct
     8041 ataagaggtt atgaaaatag tgttttgacc agagtattag agaaacaaaa cgaaaaaata
     8101 gctccatgta ttttaccgtt tttgaactaa tgtcgaagat tagctgattg aataccattt
     8161 atcaagaagt tatggaaaag ccggttcttt tttactaact gaaaaataaa attttattta
     8221 gagatcaagc tacagttgat aaatgatata aaaaagagaa tttcccatga aatagcgagg
     8281 ttgtgacaga agtgggcagc ttcaagaaat aagaaggaat ttacgaaaat tgcttttcaa
     8341 atttttgtga aatttcggct tatttttcga agaagttact tctggaacac cgtgtatcag
     8401 aggttg
//
LOCUS       DBNICI010000119         8352 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.120, whole genome shotgun sequence.
ACCESSION   DBNICI010000119 DBNICI010000000
VERSION     DBNICI010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.120"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..149)
                     /locus_tag="AC1ZCC_002144"
     CDS             complement(<1..149)
                     /locus_tag="AC1ZCC_002144"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1630379.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="AC1ZCC_002145"
     CDS             complement(593..1714)
                     /locus_tag="AC1ZCC_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HIQ1630380.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="AC1ZCC_002146"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="AC1ZCC_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HIQ1630381.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="AC1ZCC_002147"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="AC1ZCC_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HIQ1630382.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="AC1ZCC_002148"
     CDS             complement(4304..5215)
                     /locus_tag="AC1ZCC_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HIQ1630383.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="AC1ZCC_002149"
     CDS             complement(5232..6239)
                     /locus_tag="AC1ZCC_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HIQ1630384.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6557..8344)
                     /locus_tag="AC1ZCC_002150"
                     /pseudo
     CDS             complement(6557..8344)
                     /locus_tag="AC1ZCC_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
BASE COUNT         2563 a         1688 c         1461 g         2640 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aa
//
LOCUS       DBNICI010000120         8292 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.121, whole genome shotgun sequence.
ACCESSION   DBNICI010000120 DBNICI010000000
VERSION     DBNICI010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.121"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(47..1513)
                     /locus_tag="AC1ZCC_002151"
     CDS             complement(47..1513)
                     /locus_tag="AC1ZCC_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HIQ1630385.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1900..2496
                     /locus_tag="AC1ZCC_002152"
     CDS             1900..2496
                     /locus_tag="AC1ZCC_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIQ1630386.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(2554..3036)
                     /locus_tag="AC1ZCC_002153"
     CDS             complement(2554..3036)
                     /locus_tag="AC1ZCC_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630387.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3142..3339)
                     /locus_tag="AC1ZCC_002154"
     CDS             complement(3142..3339)
                     /locus_tag="AC1ZCC_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630388.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3634..4392
                     /locus_tag="AC1ZCC_002155"
     CDS             3634..4392
                     /locus_tag="AC1ZCC_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HIQ1630389.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4452..4880
                     /locus_tag="AC1ZCC_002156"
     CDS             4452..4880
                     /locus_tag="AC1ZCC_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630390.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4926..4998
                     /locus_tag="AC1ZCC_002157"
     tRNA            4926..4998
                     /locus_tag="AC1ZCC_002157"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4959..4961,aa:Gln,seq:ctg)
     gene            complement(5148..5519)
                     /locus_tag="AC1ZCC_002158"
     CDS             complement(5148..5519)
                     /locus_tag="AC1ZCC_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HIQ1630391.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5734..5919
                     /locus_tag="AC1ZCC_002159"
     CDS             5734..5919
                     /locus_tag="AC1ZCC_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630392.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6002..6190)
                     /locus_tag="AC1ZCC_002160"
     CDS             complement(6002..6190)
                     /locus_tag="AC1ZCC_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630393.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6214..6477)
                     /locus_tag="AC1ZCC_002161"
     CDS             complement(6214..6477)
                     /locus_tag="AC1ZCC_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630394.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6635..6892
                     /locus_tag="AC1ZCC_002162"
     CDS             6635..6892
                     /locus_tag="AC1ZCC_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HIQ1630395.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6963..7154)
                     /locus_tag="AC1ZCC_002163"
     CDS             complement(6963..7154)
                     /locus_tag="AC1ZCC_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630396.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7295..7615)
                     /locus_tag="AC1ZCC_002164"
     CDS             complement(7295..7615)
                     /locus_tag="AC1ZCC_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630397.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7638..8072)
                     /locus_tag="AC1ZCC_002165"
     CDS             complement(7638..8072)
                     /locus_tag="AC1ZCC_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIQ1630398.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
BASE COUNT         2630 a         1636 c         1313 g         2713 t
ORIGIN      
        1 acaggaactg ctgcaaataa gcaattcctg tttttatgtt cattgattaa tgaatagagc
       61 ccattagttt tttgattggt tctttcacag taaatagcaa gataccaccg acgattgcta
      121 ctgcaccgat aataccgaag taagcagatt ctgttcccgg tgtgtagaag cgtgcaattt
      181 gtgcattgat tgcttgtgaa gaagcatcag ctaagaacca aatagcgatt gtttgtgatt
      241 caaatgctcg tggtgctaat tttgttgtca cagacaaacc aactggtgaa agacacaatt
      301 cagcaataac cacgatgaag aaacttccca acaaccacaa tggacttact tttccacttg
      361 taccataaag catacccggc aacatcaata gcaagtacga taatcctgca aataaaagtc
      421 caagagagaa tttcactact gtagatggtt gacgttcacc aagtttcgtc cacatcgtta
      481 cgaatactgg tgtcagaata aagatgaaaa ttggattcaa tgattgatac cagcttgctt
      541 ggatcgttac accaaatagc gtagattgtg tacgttcact agcaaataaa gctaagatag
      601 atgaaccttg ttcttgtaat gaccagaaaa tgattgctgc aaggaataat gggatataag
      661 cccatactct tggtttttct tcagcagtta catcttttga agtcagcatt ttgataaagt
      721 aatacaacgg taagataatc cccaatacac tgattgtatt gacaaaaaag tcaattgtca
      781 gatgaccagt tagttgtgcc ccgccgaata ctgcagcgat aacaacaagt gcgatgatca
      841 agttacggat gaatccttta cgttcttcag ttgtcaaagg attcggcggt gttgtaccca
      901 ctccgtctaa tgtcttgcgt ccttggatga tgtattgtaa taatccaaag aacatcccaa
      961 tagcagctaa agagaaccct aaatgatagt tatattcttg accgattgta ccaacgatta
     1021 gtggtgctaa taatgcccct aagttgacac ccatgtaaaa aatcgaaaat cctgcatctc
     1081 ttcgcaagtc agtctttgaa taaagatgtc cgaccatgcc agaaacattt ggtttcaaaa
     1141 aggcggttcc gataataatc aatgccatcg aaacaaacaa tgtacttaca ccaaatggta
     1201 aagccaagac gatgtggcca agcataatga aaactccacc gaagaataca gtgcgttttg
     1261 ctcctaacac tcggtcagat acccatccgc cgacgatact agacatgtag atcaaagatc
     1321 cgtagatcga catgatagct aatgctgttg ctcgtggtaa tccaagtccc ccgttggcaa
     1381 ctgtatcgta catataatag ataagtaatg cacgcatacc gtaataacta aatcgttccc
     1441 acatctcagt gaagaacaaa gttgacaatc ccttcggctg cccgaagaag cccttgtctt
     1501 gctgtttttc cataatctat accccaattc tttaaatttt cagaaaatat tatacatctt
     1561 taccgaaaaa tttaactaaa aaacttattt aagaaatata aactaatttt ctataaaagc
     1621 aagtaatttt taatggcaat atcataaaaa aaagaagata gagatttaat aattatatat
     1681 cagctgtttt tttcaaataa gcaaaaattc ctaaaacaca acaatgaaag cgataactaa
     1741 gaaaatcctt acataacgat ttttcttatt aaaattgttt tttgaacttc gcttattttt
     1801 tgtgagcata tctgtttact tactttttgt aaggtgctat acttcatttc gtaatcaatt
     1861 aaaaaaatgc aaaacgcaaa tcaaaggaga aattttatta tgacaacttt tactgacact
     1921 ttgaaaaacc gccgttcaat ctatcatcta ggacgtaatg tttcactttc taatgaagaa
     1981 ttaacaactt tgatcaaaga agcaattaaa gaaagcccaa ctgctttcaa tgctcaatca
     2041 acacgcgcag ttatcctttt cggagatgca catgaaaaac tttgggaaat cactgaagaa
     2101 gcacttcgcc cattgactcc agcagaagct ttcccaaata cacaaaacaa attagctggt
     2161 ttcaaaaatg gttatggtac agtattattc ttcaaagata ctgacgtggt aaaaggtcta
     2221 caagaacaat ttgaattata tgcagacaac ttcccagatt ggtcagaaca atcaaacgga
     2281 atcgcaacag ctaatacttg ggtagcatta gttgataaag gattgggcgc aaacttgcaa
     2341 cattacaacc cagttattga cgaagcagtt gcaaaagaat ggaatatccc ttcaaactgg
     2401 aaactacgtt cgcaattagt attcggttca ccagaaacac cggctgagga aaaagaatac
     2461 atgaacgatg ccgatcgctt ccgcgtattt ggataatttt atttgaaata aaaaacagag
     2521 gttgtgacaa acatcttgtc gcaacctctg tttttattct ggatcaatca taatatcaat
     2581 ctctgcctca tcgtttaagc gttgggacaa aaatggaact aattcttcta atgatgcaga
     2641 aaatttcagc atcggtcctc gccatccgtt tcggaaaaaa ttcattttta cgattttaga
     2701 accttttggc tgatataata caattttatt gatctcagaa aaaggaacac cttttttgtc
     2761 aaagctattc aaaacaagtc cttcttctgt gattcctcta ctgtccaaca aaaaactaat
     2821 aaaaatcatt gctgacaaga tcccttttgc caattcatct gctgtaggat gactgatgag
     2881 tgtataccaa atgactagtg acactaagaa gggtatcacc aagtagaccc atcgatttcg
     2941 cacagtccga taaataattt cttttcgaac cagccaaaga aataaaaaca tcacaacaac
     3001 gaataaaaac aatagaaccg taaagaatgt taccatgaca actcgcctcg ctttttcttc
     3061 tccagaaaat aggaaaagaa tactgattta ccttctcaaa gaaattggac ttgcagcacg
     3121 ccgcacgtcc aattgttttc tttattcaaa tgtatcatat tttgcaacaa ccatgcttgc
     3181 tttttcatct aataattctt ggtcctctgc agcacattgg gcaactttga ccacggcgca
     3241 gtttgtcatt tttgatacaa cttcaccaat gaccgttttt gtaaaaccaa ttggtttgca
     3301 tgcatattgt tctcctaaat taatcatttc ttccgtcatc aaaatcctcc taaactgctg
     3361 gaacttttct ccacattacc actgctattt ctttggagat ttaactcatt gatttttatt
     3421 tacagttaaa aatcaacctt acaggcatta tagcgtactg ttttcgtttt cacaattaaa
     3481 acagctgttg tatttagaag aatttttttc aaaaaattaa aacatattcc ttaaaaacta
     3541 attatttcct acatattaaa atataatact tccatttcca ataaaaatac atttataata
     3601 ggaaagataa acagctgaaa agggagattt caaatgaaga aaaaaacgtt caaaaaacta
     3661 ttgtttctag ggggcacagc tgctctttta ttaagcggta caagttatta cctacatatc
     3721 cattctccag aacatacaat gaacactgca caaaaaagtt caacacaaaa aacgaaaaag
     3781 acaaaagaga aagcagaagt tttactagat gttccattag agagccagtt tgattctcct
     3841 tctttagaaa atggctgtga agtcacttcg ctttctatgc ttttatcttt ttatggttat
     3901 gaaacaacta aaaaccagtt agctgagcaa ttggattacg tccctgtttt caatgcagat
     3961 gggactcatg gcgatcctaa tgaaggattt gttggtgata tcagtggcgg agactgggct
     4021 atggtcgtct atgttccccc agttgcctcc cttgctcagc aaatcgtcca aacggattat
     4081 cacacttttc caaaaaccga tgctcaactc gatgatatca aaaaagcact atctaaagga
     4141 aatcctgtct ggacttctgt gactatcaat tttgaagtcc cagatgaaac tgattttatg
     4201 acttggacca caaataatgg agaagtaaga gtcactcctc ttgttcatgc ttgtgtagtc
     4261 actggatatg acaaagaaaa tatttatgtc aacgatccct atggtgtaaa aaatcgagca
     4321 gtaccgatca acgatttttc ggcgattttc actgcaatgg gtggacagat gctaactttg
     4381 gagaaaagct agtaattcaa acaaaaatcc cttatactca agataccaaa cctttgaagg
     4441 gagcgtagat aatggaaaaa ttcacacata aaaaaatgga tccaaatgaa attccgatta
     4501 tttttgtccg tgactgcaag ggaaatgttc aaggtaaagt ctctattaat gagtggaacg
     4561 agcgtcgcag acctgctacc ctcaatgaac tggaaatcaa attgtatcgt caatctcttg
     4621 tttattacgc agatcaagag tacgagaaag ccaccgattt attgaaattt ctaattgctc
     4681 gaacagagta tactcgcttt gaatatattg aacgtttagc aaacatctat catattatga
     4741 atgaacctgt caaagaatat cagttattgg acaccgtctt gtcagtagca gaactgattg
     4801 cccttcctgc tggattagaa aagaaactag tacgccgcct tttacgagta aagcaacaac
     4861 tatccgatca agaaaaataa cttgcgtttt tttaggaaag tgctatgatt attttgttga
     4921 ataaatggga tgtagccaaa ctggtaaggc acttgactct gaatcaagca attgtaggtt
     4981 cgaaccctgc catcccaatt tgatgtgctt gctgaaaaaa tgaaccccct gtaaaagaac
     5041 aatctttttg cagggggatt attgtattat ttttactact tgtatttagt ttacataatt
     5101 aaattatgaa taataaaatc gggcgtattc ttaatacaaa aactgattta cccaataatt
     5161 gctaatgctt tctccggaac tttttctcta ggaacttcct catacgtttc tatgtgtgct
     5221 cctttattgc taattttcaa ataatgattt tcttttaaca cagaaagacc attaaattca
     5281 accgttcgtt ctttcccatt ggcatcagca gctttctgca cataccttgc tgtaccatat
     5341 tcatttactt cctctggttt ttgtgtcttt acatagacct ctcctttttc aactaatgga
     5401 tttagctgat caagtatacc agggatttct ccataacttc cttcagtata atatttcagt
     5461 ccaaatagca aaataccact aatcgccgcc agtccaatca aatatttaat cactttcatc
     5521 tttctacctc cttgttatat attcagtata tgggtcagtt aaagaaattc catccgtccc
     5581 aagttggaga tttttctcct actttagaag cgagaatcga gtgttttttt agtaaaaatg
     5641 atcgttattt gatattattg acgtgtggat aaatcagttt ttctcgtttt gtccatgtaa
     5701 aaaatcatgt tgatagcaaa ggatgtgttc aaaatgaatc aagaagaatt atttcaaaaa
     5761 gtcaaggaaa tgattaagaa cggcaatttc gatggcgcaa aacgatttat cgaagaacat
     5821 aaagaacaat taggacctta taaagagaaa gcccaaaatc ttttaaaaga tgtaaatatc
     5881 gacagtgtca aaaacaaatt taaaaatcta ttcaaataag taaaactttc acttttgtaa
     5941 aatcaaagga ggacaatgct gctgcatcgt cctcctttgg ttcttttctg aatacttcat
     6001 actaaaataa acgtgtcgga tcgtaatgat aagtcacatt gccgttcagt gaggcttttc
     6061 ccaccacata tttacgattc tccaacttca gccacgcttc tcgaaaagcc attaattctt
     6121 ctctttttac atcaatcttt tcgatttttc catcgatcaa atcttgaata agttgttcat
     6181 atggattcat ttttttcctc catctgctgt caatcaataa gttgtaaaaa taggttcata
     6241 tgcttttttg gtatctagat cttctctgac tttgactgtc gtataaataa ttttttctac
     6301 tgggatacta taataacttc cttcaccatt attgaatcgg tgaacgtctt tctttgctaa
     6361 aataccattt attttattcg tcagtacacg ttgttcgaca ctgttactta caacatagta
     6421 aatcacccga atattattga tcaaatgcaa atcgatttct aacactacat gatccatgaa
     6481 aatcccactc cttctttcac tttgattata gcaagtttct cctctaattt caaaatcgat
     6541 acagatttta ccggataaat ccacaaaact gcttgcgcca tttcgcacaa agtgttattt
     6601 tgtatcatgg actttattta tcggaggcaa tagtatgatc gcgatcggtc aatttgtttt
     6661 ttatattcct ttttttatta tgataagcat tcttttctat tacataaaat ggacaaaaaa
     6721 gaagttttca gttttactgg cctctttacc tgctgtatat tttacttatc aaattttttc
     6781 ttttaggcat tgggaaacaa cgtctgtatt aataacacac atcatcgaat tgacgctttc
     6841 agtaattttt cttattattt ggatctattt tttatataag aatcagaatt gaatccctcg
     6901 catgtttaaa aagaaaacga ttaaagatgc agcaaatttc tgccgatctt taatcgtttt
     6961 ttctattctg ttataccaaa aaaatgtttg aatcggtgaa ggatcgttcc ttgttttaat
     7021 tctataaatt cttgtgcgat cgggtacttt tcttccaaat tttctttgtt cttttcgatc
     7081 aggcttttcg cttcttcata ttttttatct gcacatagtg cttttacttc ctgtaatact
     7141 tttcctgctt ccatttgcct caccctcttg ccattttatt gctttaagtt ttatttaagt
     7201 tatacgcatt attctagttt catcccaaat aacctttttt catttttact cctcgaacta
     7261 atcagttttc taaaggctga ttggttcgtc ttttttattt ttcctccatt tcattctgct
     7321 cttctgttaa tttatctaat gctttgattg atgctgcggc tcttgcataa tcatagctgt
     7381 ataattccat aaatttatca ggcagaacaa accacgctgg atgataaccg ttagctgtga
     7441 cagggaatcc tttatcttgt tggttatctc catcccatct cagagcaaca cctttttttc
     7501 ctttccactt tactaaagcg atcgagtatc ccttctttgt ttgtgtgccg tcttcaataa
     7561 ttgctaaaat ttctattttt tcttttggcg cacggacttc ttgaggacga accattttct
     7621 attcctccct tatctcatta cttcacgacc aacacactgc aaggggcatg tgatacgaca
     7681 taagaagcgg tcgatccaac taatgcttgt tgtatagcac cttttcctgt cgctcccata
     7741 acgatcaaat caatattttc ttgtccaggg atgacattgg caatcaaatt tcttggatct
     7801 ccagtttcaa ctattgcgtg gatctcagca atgccgtact ctttggcatc attgattttt
     7861 ttcagcatgg ctacttctgt tctttgtttt tcatctgcat agatttttga aaaggcatag
     7921 gcactatggg tcagctcaac tttgttgatg attgacacga taaataagtg ggcttcattg
     7981 tcctttgcta agtcaagggc ttcagcgaac gctttgatcg attgtcttga tccgtcaact
     8041 gctaccatga tgttctggta tcttttactc attggaaccg cctccttttt cttttattgt
     8101 aacatctcaa gcaaaaatac aaaaaatata cgcaatatgt aaataaaatg atgaacaaaa
     8161 gcaatttctt tcaagttatt tatacatttc ttttctcatc agaacacctg ttctaatccc
     8221 attatttctg cattatgtga aattcctcat catttttcac atcgagcgaa catcattctg
     8281 ttacaaaaaa cg
//
LOCUS       DBNICI010000121         8229 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.122, whole genome shotgun sequence.
ACCESSION   DBNICI010000121 DBNICI010000000
VERSION     DBNICI010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.122"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..760
                     /locus_tag="AC1ZCC_002166"
     CDS             <1..760
                     /locus_tag="AC1ZCC_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIQ1630399.1"
                     /translation="GTGTLISEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAG
                     LVIQFSLFYSNGPSMIWFIIAGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSER
                     LRKWVDTCNQQIYSLRETVQTIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKI
                     DFLLTISFISFAVVLSGVIPTPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFI
                     LPFLYGFVYVIAERRRVIKEEIIEVKKENKENQG"
     gene            1011..3620
                     /gene="clpB"
                     /locus_tag="AC1ZCC_002167"
     CDS             1011..3620
                     /gene="clpB"
                     /locus_tag="AC1ZCC_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HIQ1630400.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(3771..4457)
                     /locus_tag="AC1ZCC_002168"
     CDS             complement(3771..4457)
                     /locus_tag="AC1ZCC_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630401.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(4435..4929)
                     /locus_tag="AC1ZCC_002169"
     CDS             complement(4435..4929)
                     /locus_tag="AC1ZCC_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HIQ1630402.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4949..6169)
                     /locus_tag="AC1ZCC_002170"
     CDS             complement(4949..6169)
                     /locus_tag="AC1ZCC_002170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1630403.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            6316..8151
                     /gene="lepA"
                     /locus_tag="AC1ZCC_002171"
     CDS             6316..8151
                     /gene="lepA"
                     /locus_tag="AC1ZCC_002171"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIQ1630404.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT         2666 a         1542 c         1680 g         2341 t
ORIGIN      
        1 tggtacagga acgctgatct cggagattta tttttataac aaaaaaggaa tgaagctatc
       61 ccagggagca ggggtgacag cactccatat gattatgtac aaactggcag tgatcactta
      121 tgcattagct ggcttggtca tccaattctc tcttttttat tctaatggtc catcaatgat
      181 ctggtttatc atcgctggta tccttttgac gatcgtgatt gttgcctttt tattggcgat
      241 gtccgtcagc ttgaatgttc aaatattttt tgtcagctta tcgaataagt tatttaaaag
      301 tgagcgtttg cggaaatggg tggatacatg caatcagcag atctactctt taagggaaac
      361 ggtccaaacc attatccaag acaggactgc gctattgcgg atttatctat ggaatatgtt
      421 aaaacttgta ttttggtatg tacttcctta tttgattctg attgaaaacc atcctaaaat
      481 agattttcta ctgaccattt cgtttatcag ctttgcagta gtgctttcag gggttattcc
      541 tacgcctgct ggtattggtt cctttgaatt tgtctatttg ttattattca gaccagttgt
      601 tggaacagtt gacgcagttt cttcgctttt actgtatcgt ttcagttcct ttatcttgcc
      661 atttctctat ggatttgtgt atgtgattgc tgaacggcga agagtgatca aagaagaaat
      721 aatagaagta aaaaaagaga ataaagaaaa tcaagggtag gtcttttata agatctaccc
      781 tttttatcaa atcaaagaaa aacgttatgg ataagataat tttacgtttg tttggctctt
      841 tttttatata catacaatca attttgtttt tttaagattt atataagtcg agaaaaaaag
      901 tcaaaaaact atgtgtatat tttgaccaaa attgaccaaa gcgatataat agtttttgta
      961 aagatcaaag aaataagtga tcttaccttt aattaggagg gtatacctgt atgaatattg
     1021 agaaaatgac tacgacgctt caagaagcga ttgctgaagc tcaacagatt gcggtcactc
     1081 gaaaacatca agatatcgac attgcccatg tatggaaaat ctttttacaa tctaatcatt
     1141 ttggtcgaaa tttctatacg gatgccggat tggatgtcga atcttttgaa catgaaattg
     1201 atcgtctact ggatgagtat ccagttgttt ctggtggaaa cgtccaatat ggacaaaacc
     1261 tcagccagaa tttctttcga ttattgaatg aagcagacca aatacgtgaa agcttcggcg
     1321 acgagtttct ttcaacggaa gtcgttattt tagcgttgat gaaattaaaa aattatccgt
     1381 taactgttta tttgaataaa aacggattat ctgaaaaaga attgcgtaaa aacatcgaag
     1441 agatgagagg aggagaaaga gtgacctcac aaaaccaaga agaacaatac aaagcattag
     1501 aaaaatacgg cgttgattta gtacagcaag tcaaaagcgg aaaaatggat ccaatcatcg
     1561 gtcgtgatga agaaatccgt gatgtcatcc gtattctttc acgaaaaaca aaaaataatc
     1621 ctgttctgat aggagaaccc ggtgtaggga aaacggcaat cgttgaaggg cttgctcaac
     1681 gtatcgtacg caaagatgtg cctgaaaatt taaaagataa aacgattttt tcattagata
     1741 tgggtgcatt gatcgctgga gcaaaattcc gtggtgaatt tgaagaaaga ctaaaagcag
     1801 tcttgaaaga agtcaaaaaa agtgatggac gtatcctctt gttcatcgat gaaatccaca
     1861 atatcgtagg ggcaggaaaa acagaaggaa gtatggatgc cggaaatctg ttgaaaccaa
     1921 tgcttgctcg cggtgaatta catttgatcg gtgccacaac tttagatgag taccgtcaat
     1981 atatggaaaa agacaaagcg ttggaacgtc gctttcaaaa ggtattagtc aaagaaccaa
     2041 cagtggaaga tacgatcagt attctgcgtg gattaaaaga acgttttgag atccaccatg
     2101 gtgttaacat ccatgacaac gcattagtag cagcagctac tttatctgac cgttatatta
     2161 ctgatcggtt cttaccggac aaagcaatcg atttgatcga tgaagccagt gcaacgattc
     2221 gggtagaaat gaactctatg ccaacggaat tagatcaagt tactagacgt ttgatgcagt
     2281 tggaaatcga agaagctgca ctgaaaaaag aatcagatga tgcaagtaaa aaacgcttga
     2341 aaaatttgca agaagaatta gctgaacttc gtgaagaagc aaatgcgatg aagatgcagt
     2401 gggaaacaga aaaagaagaa gtcaattctg tttcagcaaa acgtgctgaa atcgataaag
     2461 caaaacacga attagaagat gcagaaaata actatgattt agaacgcgct gctgtattgc
     2521 gtcatggtac gatacctcaa ttagaaaaag aattgaagga attagaagct aaagcaaaag
     2581 acagcgagat taagatggtt caagagtccg ttaccgaaaa tgagattgct caagtcgttg
     2641 gcagattaac gggtatcccc gtaacgaaac ttgtagaagg tgaacgagaa aaactaatta
     2701 aattgaatga aacacttcat aaacgagtga ttggtcaaga tgaagcagtt gatgcggtca
     2761 gtgacgcggt aattcgttca cgggctggat tgcaagaccc aaatcgtcct ctaggttctt
     2821 tccttttcct aggaccaact ggtgtcggaa aaaccgaatt agcgaaagcg ttagctgaga
     2881 acttgtttga ctcagaagat catatggtac ggatcgatat gagtgaatat atggaaaaac
     2941 atgctgtgtc tcgtctagtc ggagctcctc caggctatgt aggttacgaa gaaggtggtc
     3001 aattaacgga agctgtcaga cgaaatcctt atacaatcgt cttgttggat gaaatcgaaa
     3061 aagcgcatcc cgatgtcttc aatatcttgt tacaagtcct agatgacgga cgcttgactg
     3121 attcaaaagg gcgagtagta gatttcaaaa atacggttct gatcatgacc agcaatatcg
     3181 gttcacagtt actgctggaa ggcgtgactg ctgatggaac gattccagaa gcagtggctg
     3241 aacaagtcaa cacgctatta cgtggaaact tcaaaccaga attcttgaac cgaatcgacg
     3301 acacgatttt atttacacca ttgagcctag acaacgtgaa aggaatcgtt gacaaaatgg
     3361 tggcgcaatt ggcacaacgt ctggaacatc aagaaatcct gctgacaatc agtgatgaag
     3421 ccaaaacgtg gattgctgaa aatgcttatg aaccagcata tggtgcaaga ccattgaaac
     3481 gatttatcac taaggaagta gaaacaccac tagctaaaga aatcgttgca ggacatgtta
     3541 tgcctaaatc aaaagtaacg attactttac ttgatggaca acttcatttc aaaacagaag
     3601 agctagaaga aatcgtctaa tcttccattg agactataaa agaaaaaagg ggtctgacac
     3661 aactagtgtc agacctcctt ttttcaaata aacagtgttc cagaagtagt ttcttcgaaa
     3721 aataagccga aatttcttga agctgcccgc ttctgtcaca acctctttta ttaggtattt
     3781 ttcacttttt ctaatttata actttcaaag taacgctcat cttttttact ttgagaccat
     3841 gcaaacaatc ccattaaggc agcacctatc gtatttgtca gcaagtcgcc catcgtatcc
     3901 atcaatgctg ctcgtccgac aaacagtgtg ccatcagaag cagcaaaacg ttgaagattc
     3961 atgcctaaga actggtcgca taagaattcc cagaactccc aaaacacgcc gcaaagtcca
     4021 gcaaacgcaa agccgaataa gaggaacaac caaggactgg tttttgatat ctcagcatct
     4081 ttcatcaagt aaccaatcaa tccatagcca agagctgtca ggaccattgg actcaccgca
     4141 tgtaagattt tgtcccaaaa agagatgata ctgatcaaat gcatccctgt gcctaaaaag
     4201 acagagatga acaagaaaaa ccagtaaaac aaaacgattg ctgctggtat tttaagtttg
     4261 aaaactttca aaatgacttg tggcatgaaa atcaatgcaa tccctaataa agattccacg
     4321 ataaatgtga ttccttgaga agtcgaatat ttttgtgtga taaattcata aaaattatag
     4381 cagacaacga atacaccaaa agcaagtaaa gctatttgca cctttttgat ttctttaatc
     4441 actgaaagtc cctccatcta aaaatctttt tacttctgaa agcaactcaa tttctgaaga
     4501 aaccactttt ccgttcaatt tgatcaaacc tattgtatat aagttcaggt agtgaaattg
     4561 attttcagct gtttcttgta gtgcagcgat ttttttcggg tgggttccgc cttgctgtcg
     4621 agtatcagta tataagccga tcactgggat gcctttcgca taagccacac cgatttccga
     4681 tgctaccccg gcatcgatcg tcagcccatc tagtaaagca atcattaaat cactagccaa
     4741 cactttttct gtgtcagcaa gagcaatcat tcgactgtct gcataagcag ttttgtcatt
     4801 gattgcttca ttttcttgag gaagatagat cgaaagctca gaagagagct ctctgatttc
     4861 tttaactaac tgttggttat ataacaaatc acttttagca aacattggtg cggcaaaata
     4921 aattttcatc taaacatcat cctttttatc aaaaataatc gctaaagtaa gcttccatgt
     4981 ttggaatgat acgctctagt gacttcagtg tttcttcatc tgtttctaac cgttcgattc
     5041 ttgccaaata agaagcacgg cgataattca tcatcaagca agtcaatgtt tggatatcca
     5101 gtcgaacgga ttttcctata ggttcattta agacacggac ttgtccctgt tcatcccatg
     5161 ttaaagcgaa gacaccatta ttccaaggag cgacaggatc ttcaatgatg aaatgaaaag
     5221 catcagctgt tccaacaaac ggaaaccgtt gaaggaattc ttttacatca acgatccgag
     5281 ccataaaata tggttcgatc tgctctttga tctgactatc ttcaatcaaa aatgctaaag
     5341 gctcattttt ataaatatct ccatgtaccc agtagatcat ggaaaaatgt gcggaaataa
     5401 agttccataa gccatttcga gcttcttgat caagatagaa catttctttt acgtgaaata
     5461 tttcttcagc tacccagtag aacagaacac ctcttggttc atggttgcta tcgtagtaga
     5521 cagcggctgt gcgctcatct tcattttcga aacgccaata ctcttcccaa tgaaaatcac
     5581 tgcggaataa agcaccatgg ttttgttggg caaatttgtc ataaacggaa aatacgtctg
     5641 gatgatcaac tggtaatcgc tcaaccatac cacttactgg gatttgttta ggtagctgtg
     5701 tatctctgat tttaaaggaa agtttatcag acatgatttc ccatccttta cgccgatagt
     5761 aggggatact gtaaggatac aaataagaga tccattgctt attcttgcgc atattatcca
     5821 atgcaacgat aattagatct tgcatcaatc catggttagc atattctgga taagttccaa
     5881 ctccagtcac accacccatt ttataacgtg caccgtgaat attgacttca caaggataga
     5941 tagcgatttg agagatcagt ttattttcgt ggaaccatcc gaacactttc gatacttcca
     6001 aaatcggttt tttggaacgg ataaaagctc gtttattctc aaacccgctt tcctcgatgt
     6061 ctgcttcagt cacttggaac acataggaaa gcaattcatt gaattgatcg atatgttctt
     6121 gagtgaccgg ttttaattca agattttctc taaaatcttt ttgatccatt ttttcatctc
     6181 catttattag cttgataaca gtatagcaaa attctacaaa aaaaacttct atagctgact
     6241 aattaaaaac tctttaaatt actgcttttc gttggtataa tggaaagtgc tgattatatg
     6301 aaaagaggag acgctatgga tataaagaaa atgaaagaac gccaagagca gattcgtaat
     6361 ttttcgatca tcgctcatat cgaccatggg aaatctacat tggccgatcg tattttagaa
     6421 atgacgcaca ctgtgacgtc tcgcgaaatg caagatcagc tgttagactc gatggatttg
     6481 gaacgtgaac gtggaatcac gattaaatta aatgctgtag aactcaatta cacagcgaaa
     6541 aatggagaaa catatatctt tcacttgatt gacaccccag ggcacgtcga cttcacctat
     6601 gaagtctctc gaagccttgc tgcctgtgag ggtgccgttt tagtggtcga tgcagcacaa
     6661 ggaatcgaag cacaaacatt ggccaatgtt tatttagcgt tagacaatga tttggagatc
     6721 ctgccagtca tcaataaaat cgacctaccc gcagcagatc ccgaacgtgt ccgtcaagaa
     6781 atcgaagacg taatcggaat cgatgctagt gaagctgtat tagccagtgc caaatcaggg
     6841 attgggattg aagatattct cgagcagatc gtggaatatg ttcctgcacc aagtggagac
     6901 ttagatgcac cgctaaaggc tttgatcttt gactctattt atgatagtta ccgcggtgtt
     6961 gttctaaatg tccggatcac ggacggtatg gtcaaacccg gagacaaaat caaactgatg
     7021 agtaatggca aaacatttga tgttacagag gtcggggtct tctcgccaaa agcagttgct
     7081 agagactttt tgatggtcgg tgatgtcggt tatatcacag caagtatcaa aactgtccaa
     7141 gatacacgag taggggatac agtaacgtta gcagacaatc ctgccgctga agctttacca
     7201 ggttaccgaa aaatgaatcc gatggtttat tgtggtcttt atccaattga tacttctcgt
     7261 tacaacgatt tgcgggaagc tttagaaaaa ctgcaattga atgatgctgc tttacaattc
     7321 gaaccagaaa cctctcaagc attaggtttt gggttccgct gtggattcct cggacttctt
     7381 catatggatg tcgtccaaga acgcttggaa cgggaattca atctagaatt gatcacgact
     7441 gcaccatccg ttatttacca tgttaataaa acggatggta cgactgctac agtagataac
     7501 ccggcagatt ttcctgaacc tgtaacgatt caggatgtag aagaaccatt cgtcaaagca
     7561 cagatcatgg tcccaaatga ttttgtcggt gcagtaatgg aactttctca acgaaaacgg
     7621 ggagaattca ttacgatgga ttatttagat gattaccgag taaatgtcgt ctataatatt
     7681 ccgctttctg aaattgtctt tgactttttc gacaaattga aatcaagcac gaaaggctat
     7741 gcctcattgg attatgaaat gtctggttat caaaaaagca agttagtgaa gatggacatc
     7801 ttgctgaatg gagaaaaagt cgatgcatta agctttatcg ttcaccgaga ctttgcctat
     7861 gaacgtggaa aagcaatcgt tgaaaaactg aaaaaactaa tcccacgcca acaatttgag
     7921 gtgccaatcc aagcagctat tggacaaaaa atcgttgccc gttcagacat caaagccttg
     7981 cgtaaaaacg tcttggctaa atgttatggt ggagatgttt ctcgtaaacg taaactctta
     8041 gagaaacaaa aagaagggaa gaagcggatg aaacaaatcg gctccgtcga agttccgcaa
     8101 gaagccttca tggccgttct gaaaatggac gaggacgaac cgaagaaata gcaagggttt
     8161 gtttgaaaac agtgtaaaaa ataaaccatg tttacatgga tttgccaccg gtttgccacc
     8221 ggtggtttt
//
LOCUS       DBNICI010000122         7978 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.123, whole genome shotgun sequence.
ACCESSION   DBNICI010000122 DBNICI010000000
VERSION     DBNICI010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.123"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="AC1ZCC_002172"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="AC1ZCC_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HIQ1630405.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1227..2330
                     /locus_tag="AC1ZCC_002173"
     CDS             1227..2330
                     /locus_tag="AC1ZCC_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HIQ1630406.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(2327..2533)
                     /locus_tag="AC1ZCC_002174"
     CDS             complement(2327..2533)
                     /locus_tag="AC1ZCC_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630407.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(2523..3590)
                     /gene="rlmN"
                     /locus_tag="AC1ZCC_002175"
     CDS             complement(2523..3590)
                     /gene="rlmN"
                     /locus_tag="AC1ZCC_002175"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HIQ1630408.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3820..6267)
                     /locus_tag="AC1ZCC_002176"
     CDS             complement(3820..6267)
                     /locus_tag="AC1ZCC_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIQ1630409.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            complement(6456..7892)
                     /locus_tag="AC1ZCC_002177"
                     /pseudo
     CDS             complement(6456..7892)
                     /locus_tag="AC1ZCC_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
BASE COUNT         2214 a         1736 c         1357 g         2671 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta cttataaaat aaaaaattat acagaaacac tttattcaaa ataggaaaga
      841 tcaggaaagc catcaattgg aggaaaaaac tctgtttgtt catcatgata tagattatct
      901 tttgctttaa aaaaaaccaa ttatgaattg acaaataaaa cctgttttca tataataatc
      961 ttaattttta gaatttttag ataattacaa aaaagcaaag ggaatcggta gaaatactaa
     1021 gaggaacaaa aattcattat tgtcgttttc aatgtttttg tacttgttcg tttttaggat
     1081 acttattgat gatttcaagg tgttcatgcc accttattca tcacttatcc tgtcttaggt
     1141 tcacctctac tccatctaca gccgcagtca gatcgatcac tctttaaaat tttaacccgt
     1201 ttcaacgaag aaagaaggaa atgaatatga gtcaaagtgt tttcttacca aattatacca
     1261 ttggaacaga tgcgtatgaa aaagcaaatg gaatcgtcaa agaatatggg acaaaagtag
     1321 tgctgattgg agggaagact gcactttcaa aggcagaaaa caaaataaaa gtagctttga
     1381 cacaggaaat agaggttgtc gccagtctct ggtacggggg taattcaact tatggaaata
     1441 ttgaacagtt gatccagcat gaaagtgtcc aaaaagcaga cgtcctgttt gcagtcggtg
     1501 gtggccgtgc ttgcgatact gtaaaagtca ttggtgaaaa actagctaaa ccagtcttca
     1561 cctttcccac tattggttcg aactgttcgc ccgtcactgc ggtatgtgtc atctataacg
     1621 aagaaggcgt aatggacgga ctcttctttc cttcaaaacc acctgtacac accttcattg
     1681 atacagcaat tattgccgaa gcacctgata aatacttatg ggcaggtatt ggcgatgctt
     1741 tatccaaaga agtcgaatct actttttctg ctcgtggcga tgatctaggc tacacggata
     1801 gattaggaat ccaaatcgga aaaatgtgca ttgatcgtct cttgacttat ggtacgcaag
     1861 cattgaaaga tagtgccgac gatcgtccct catatgcaat tgaacaagtc gtttcggata
     1921 tcattgttac taccggattg gtttctgttt tagttaacaa taactataat tctgctatgg
     1981 cacactcatt ctattacggt acgactgttc tagaaaaaat ggagggatac cttcacggag
     2041 catctgtcag ttatggtgta ctgaccttat tgatgatgga tcagcagttc gatttgtttg
     2101 ataaaatcta tgtgtttatg aaaaagaacc aacttccagt atcgttaacc gacatgaatc
     2161 tcaatgtcac gaccgattta gaagcaatct tagataaagc catggcaaca aatgatatta
     2221 aacatgtgcc ttatgaaatc acccgagaaa aatttaaaca ggccattctt gacgtagaaa
     2281 cttatcatgc tacacaagaa agccagcaag gatataagaa aatcagttaa ataattgcta
     2341 aaacccccat caagcaggct gtcatcaaat tgaggacatt tctttgcttg atgagggttt
     2401 ttcttgtcaa aaaaagcttt atagctttcg ctatagaatc ttttctctta tttaagttga
     2461 acagttcctt aattaagaaa actgctcctt ttatggtaat tgaaaaaacg agtaggatac
     2521 gattatttgg caactgtttt tttcatttgc ttgcttcgca attgtccgca agctgcatca
     2581 atatctgtac catgttcttt acgtatcaca cagtttacgc cgtttttctt caatacatca
     2641 taaaatctca atacatcagc ttttggactg cgagaatatt gatcatgttc actgactgga
     2701 ttatatggaa tcaagttgac atatgctaat tttttcttat cttttaatag atctgctaat
     2761 tgttgcgcat gctctggtct atcatttact tgattcaaca tgatatattc aaacgtgatt
     2821 cgacggtttg ttttctctaa atactcatcc acagcttcca tcaatttctc aattgggaaa
     2881 cttcggttga tccgcatgat cgatgttcgc acctcattat tcggcgcgtg caaggaaata
     2941 gccagattga cttgcaagcc gttattagcg aattccttga ttttgtgtgc taatccacta
     3001 gtggaaactg taatgtgccg agcaccaatt gccaatcctt tggcatcatt gatgatatgc
     3061 aaaaagtcca ttacattgtc gtaattatca aatggctcac cgatccccat tacaacgaca
     3121 tgtgaaacgc gttcgccaag attgcgctca tcaaaataat gttggaccat catgatctgt
     3181 gcaacaattt ctccagcagt cagatcacgg ttctttttga gcagcccgct tgcacaaaac
     3241 gtacagccga tattacatcc tacttgtgtc gtcacacaaa ctgacaagcc atattcttgt
     3301 ctcatcagta cagtctcaat catgttttta tcagggagtt caaacaaata ttttactgta
     3361 ccatccgaag cttcttggac gaccatctgt tttagaggat tgatgataaa atgctccgat
     3421 aatttttcga tcaattgttt cgatagattc gtcatttctg tgaaattact tacacgtttt
     3481 tgatacaacc attcccaaac ttgggcagct cggaattttt tctcgccgtt ttctataaac
     3541 caattgatta attcttcatt cgtcaagcca taaatggact gtttttccaa agtattttcc
     3601 ctctttttct caaagtttcg ctacatacta tacatgattc atttccttaa tgcaatactt
     3661 cacaaataaa tcgaatagaa ctttaatctc acaaaaacta aaaagacaag acatcatacc
     3721 gtattagaaa agatggaaaa aaaccatttt ttcttttggt atcatcatgc cttgtctttt
     3781 tattggttat tcgtttttca acccttcatc acacaagttt tacatgtcat tatcgtccgg
     3841 caacgtttct gatgtttctt cctgctcaat gaatactttc cgaggcttgc ttccttctga
     3901 cgggccaaca actccatgag cttccagttc atctactaaa cgtgctgctc ggttgtaacc
     3961 gatccgaaat cttctttgga gaagggaaat acttgccgtt tgcatttcaa cgacaagtgc
     4021 ttttgcttct tcaaacagct catcttgtgg ctgttctgga gcacctgtcg tttcaacttc
     4081 atctgtcggc atcattttct cttcataatg agcttcttgc tgatctgtaa caaattgaac
     4141 gacccgttct acttcgtggt cagaaatgaa tgctccttgc acacggatcg gtttattctc
     4201 acccattggc aagaatagca tatctcctcg ccctaagagc ttttctgccc cattagaatc
     4261 aatgatcgtc cgagaatctg taccgcttga taccgcaaat gccattctgg aaggtacatt
     4321 tgctttgatg attcctgtga taacatccac acttggacgt tgagtagcta ggatcatatg
     4381 aatccccgct gcccgagcca tttgcgctaa acgaataatc gcatcttcaa cttcattgct
     4441 ggctaccatc atcaaatcag ctaattcatc tacgatcact acgatgaatg gtaaggttgg
     4501 atgtttcgtt ccgttttcca agtttttctg ttgaacaaat tcattgtaac cagaaatatt
     4561 tcttactccg gttgccgcga atttttcata tcgttcttcc atttcttgta ctactttttg
     4621 taaggcttgc gctgcttttc tagggttcgt cacgaccggc gtcaacagat gaggtatgcc
     4681 gttgtacata ttcaattcaa ccattttcgg atcgatcatc atcagtttta cttcatgggg
     4741 cttagcacgc attaatatac ttgtgataat cccattaatt gctactgact tcccactacc
     4801 agtcgaacca gccacgagta agtgaggcat cttcgaaaga tcagccgttt gtacccgtcc
     4861 agaaacatca cgtcctaaag gtacttctag taatttatct gggtggtttg gttgtgcttc
     4921 gatgatttct ctaaaggaaa ccatactgat ctttccattt ggtacttcaa taccaatcag
     4981 tgatttacct ggaatcggtg cttccatccg gacatctttc gccgctaaag caagcgcgat
     5041 atcatctgtt aagttcacaa ttttacttac ttttacccct acagccggct ggacttcgaa
     5101 ttttgtgacg gatggtccta agctggcttt gacgaccttg gcatcgacac cgaaactttg
     5161 gaaagtttgt tctaatacgc cgatattttt ttcaattttt ttatactcat cactttgatc
     5221 cacagttggt atagaatcaa gcaaatcaac tgtcggtaat tcatagtctt gattttcctg
     5281 ttctgcttca atctcaaatt ctaatgaggt accgtcatcc tctggcaact catctgaaaa
     5341 accttctgct gtattggtct gttttggtgt atctggtact ggtttctcta gattcgcagg
     5401 atggatattt tcttgaaaac tgtcgattgg aacaaaactt agctgctccg gctctgtatt
     5461 ttctggttcc tgccattcat cagcagtatt ttccctttgc tggctcaccg cttttttctt
     5521 ctcatattct gcagcttctt tttctgcagc ttcttttgca gctaagcgcc ttgcttcttt
     5581 cgcttctgcg cgctgtttca ttctctcttc ctttgccgct tgctttcgag cttgcttggc
     5641 aggatcacct tccaataatt cctgtactcg ttctccaatt gattggatac catttaacag
     5701 ctgatagcct tccagattac tgaacaggaa tgctcctcct gccagtaata gcactgctgc
     5761 taaataactt ccaggctgtg caatcaagaa gtaagtaaaa tgataaagga ttgctccaat
     5821 catcccacca ccaacgttat tgtctacttg actttgtttt atatcactgg ctaacaaatc
     5881 ccatgtcgtc cccatgatat ttggttcccc agtatggagt ttgccaaata aatgggcatg
     5941 taacaataat aagataccga aataaaagag tatgccgcca aaccagcgtc ggtttttacc
     6001 aattgaaaac tctgtattct taatgacaat ccaaaatcct aatacggcta ataaaagaca
     6061 gaggatttgg taagtatttc ctgcaatgat ccgaaagccg tttgcaatca atattcctaa
     6121 aaaacctaat tttaaaaagc cgaagacagc aaataaaatc aaggcaaacc caacaccaat
     6181 cgtcagcatt tgttcctgct gctgtttttg ctttttcgtt tttttcgtct ttttcctttt
     6241 tttagtaggc cgtttctttt gtgccattta ttatccctca tttcatcaag tttcattata
     6301 caaaaaaaag taagggaact aaagttttgt ttgaaagaac tgacgctttc tttcattttt
     6361 cagcttattt tacagttttt cttaatttct aaatgatttt atcaagaaaa ggcacggcca
     6421 tatccagccg taccttcttg ttttaagtat gtgaactaaa ggttctctcc attttcttca
     6481 atcagttttt ggtaccagta ataactgtct ttcttatatc gttccaaaga tccttcttgt
     6541 gtttcgtctt aatcaacata tacaaatcca tatcgctttt gataaccgtt cagccagcta
     6601 agcaaatctg tataagacca ggtgcaatag cctaggactt ctgctccatc cgaaattgct
     6661 tctttgatgg ctttgacgtg gctttgtaag taattgattc gatagtcgtc atggatttgt
     6721 ttatcttctg tcaacttgtc atactctcct aagccatttt ccgtaatcag taccggcatg
     6781 cggtaacggc ttgtgatacg acgcagtccg atccttaatc cttctgggtc aatctcccag
     6841 tcccaatttg ttcgttcgac aaatggattc tcaacttttt tatacattcc aggaactcca
     6901 gtttcttctg atgaaccttt ttttcctgtc gtattcattt ttccagcacc aactccgttt
     6961 aatggattga atgcattcgt cgccgtttga tagtaattga tcccaagaaa atctggttta
     7021 gcagattcta gcaattctgc atcgccagct tcaatcgttg gtgcccatcc tttttcttct
     7081 aagaacctca tggcagcaat cggatatttt ccccacatat agacatccat ccaataattg
     7141 gccatcaggt cttctgcatt ttcagcagcc agaacatttt caggattact gtcaattgga
     7201 taatttggcg tatacgcaaa gcttggtccg atttttccag gcatttctaa ctcgtgaaat
     7261 ttattgatga cagatgcatt tgctaaatta gcaatatggt taacttcata cattcgtttc
     7321 gggtctgtca cacctggcgg atgtgctgct aacaaataac ctaatgaagt gaagatattt
     7381 tgttcattca aactaatcca gtaatggact ttcccgcgga atgcctcaaa taatgtttct
     7441 gcataatgag tgaaatcttc aatgatctga cgtgattccc aaccattata ttcatcttgc
     7501 aatgcttgtg gaagatccca atgatataaa gtcaacatag gttcaatatc gttttcaatc
     7561 aattcgtcga tcaatctctc ataaaaagcg agcccagctt gattgacttc tcctcgtcca
     7621 ttaggaaaaa tccgtgtcca agctacagag aagcggtaag ctttcagacc ttgttctttc
     7681 attaaagcaa tatcttcttt ataacgatga tagtggtcta ctgccaaatc accgttcgtt
     7741 cctttaaatg tctttccagg tatccgtaca aatcgatccc agactgattc tcctttccca
     7801 tcttcttgcc aagctccttc tacttgatac gctgcagaag cagagcccca cagaaaatct
     7861 tttggaaatg aggtaagttc tttgtgttcc attcatctct tcctttccta taaccttcaa
     7921 tcgttcctct tttagaataa cacgaaaccc cttccacaac aaaataaaat tatgtaaa
//
LOCUS       DBNICI010000123         7777 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.124, whole genome shotgun sequence.
ACCESSION   DBNICI010000123 DBNICI010000000
VERSION     DBNICI010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.124"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            337..705
                     /locus_tag="AC1ZCC_002178"
     CDS             337..705
                     /locus_tag="AC1ZCC_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HIQ1630410.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            885..1826
                     /locus_tag="AC1ZCC_002179"
     CDS             885..1826
                     /locus_tag="AC1ZCC_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HIQ1630411.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1895..2626
                     /locus_tag="AC1ZCC_002180"
     CDS             1895..2626
                     /locus_tag="AC1ZCC_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIQ1630412.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2770..4950
                     /locus_tag="AC1ZCC_002181"
     CDS             2770..4950
                     /locus_tag="AC1ZCC_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HIQ1630413.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4970..5452
                     /locus_tag="AC1ZCC_002182"
     CDS             4970..5452
                     /locus_tag="AC1ZCC_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HIQ1630414.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5468..6229
                     /locus_tag="AC1ZCC_002183"
     CDS             5468..6229
                     /locus_tag="AC1ZCC_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIQ1630415.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6360..7694
                     /gene="murC"
                     /locus_tag="AC1ZCC_002184"
     CDS             6360..7694
                     /gene="murC"
                     /locus_tag="AC1ZCC_002184"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIQ1630416.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
BASE COUNT         2608 a         1351 c         1702 g         2116 t
ORIGIN      
        1 cactctgagt cacaaagagc aatttcaaaa aatagcttct catgtttgtt gaatattgtg
       61 tcttgtgacc gaggagttgt caggagaaca ccgtttacca gaggttgtga cttgttttta
      121 gtcacgacct ctttttttat ttattccata taaatacaca ttatttattc cgaaaagtga
      181 aagcgcaaat aacaaaaaca accaacttat ttattattaa agaaaagcgt atttaagtag
      241 gacgattctg ttgagagctt tcgtagattt tcccattttc ctatggtaaa ctgacaaagt
      301 tgagaatgtt agaacagatc gtttaggagg aaacagatgt ttacgaatga aataaaaatc
      361 atgctttatg tggacgatgt agaaaaaaat gcaacgtttt ggagagcagt tggctttgcg
      421 gaaaaagatc gtcaagaaat ggatgggacg cttataattg aagtaggagt tgaggacagt
      481 caaacctcat ttctgttata tgatcgagca tttgttgagt cccattcacc tggcacagct
      541 actagtgccc catctttgat gttttacagt aaagatatct ttggactata taaaaaaatg
      601 cagacgcaat cagtcacatt aggtgatatg gtacagcttg gagaagaata tgtctttaac
      661 tttgctgatc ccgatggcaa ctactttgcg gttacaggta aataatagaa ataaagactt
      721 tgtttttctg tgcgaaacag gaaagcgaag tctttttaat tcaaataata atattatgta
      781 aacaaaaaac tttccatgcg taattataca caagcaaata aatcgtcaaa tgcgaacgcc
      841 ttctttatac tgtttaggaa cgaaaagaag gaaggaagaa atacatggat tatcaagtac
      901 tgctttatta caattataca aaaatcgaag aaccagagga atttgccaaa aatcatttag
      961 ccttttgcaa atctttggat cttaaaggtc gtattctagt agctgaagaa ggaataaatg
     1021 gtactgtgtc tggaaccgtt gaaaacacga ctgcctatat gaaagcaatg aaagctgatc
     1081 ctcgatttgc tgatacgatg ttcaagatcg atccaagcga aggacatgcc tttaagaaga
     1141 tgtttgtaag ataccgtcca gagcttgtaa gtttaaagct agaagaggac attgatccaa
     1201 accaagtgac aggtgcctat ctttcaccgc aagaatttaa agaagctatt ttagatgaac
     1261 agacagtggt gattgatgca cggaatgatt atgaatatga cttgggacat tttcgagggg
     1321 ctgtacgccc agatatacgc agttttcgag agcttccaga ctggatccgc gagcacaaag
     1381 aagaatttat ggaaaaacgg gtagtgacct actgtactgg tggcattcgt tgcgagaagt
     1441 tttctggttg gcttgtccgt gaaggattca aagatgtcgg acagctgcat ggcggtattg
     1501 ctacttatgg taaggatcct gaagtccaag gagaattatg ggacggcaag atgtatgtgt
     1561 ttgatgaacg gatcgctgtc gatatcaacc aagtagataa acaaatcgtc ggtcgcgact
     1621 ggtttgacgg aacaccatgt gaacgatata tcaattgtag caatcctgag tgcaatcgtc
     1681 agattcttgc ttccattgaa aatgaagaaa aacatttagg agcatgtagc atagagtgtg
     1741 cgaaacatcc gcgcaatcgc tatgttcaag accatggaat ctctccagaa gaagcagaac
     1801 tgcggtttac agctttaatt aaatagaaaa caatagtttt gattgtcgac catttccaat
     1861 atgcgctata ctagcgttga ggatggtgat ccacatgaaa tcattgattt atgctattgg
     1921 ggatgtccat ggcaaacatg agctttttga aaaacttatt cactattatg atccaaagat
     1981 ccatcagctt gttttattag gggatctcaa tgatcgaggt cctcgtacaa aagaatgttt
     2041 tttattagga aagcaattag tcgaagaaac aggagccatt tacctccggg gaaaccatga
     2101 agaatatttt ttgcaatttc tgcaatcacc ggaggactgg tatgcgcctt atatgagaaa
     2161 tggtggaaaa gaaacgtttg aaagcctgat tcacccaggg gcatcggagg aatactcccc
     2221 aactgaaatc tcgatgatga tccaatcgag atattcagaa ctgattaaat ttttagtcca
     2281 gcgccccttg tattatgaat gggaaaatta tctttttgtc catgccggtg tggatttgac
     2341 aaagaaagac tggcgtaaga caagtctaag agactttatt tggataaggg aaccatttca
     2401 tgaagggaaa aacaatacag gtaagacaat tgtatttgga catacgatta caccaatgct
     2461 ccatggagat atgcagacaa cagacctctg gattcaggat cataaaatcg gaatggacgg
     2521 tggggctgta ttcggagggt cattgcatgg ggttgttttt gacaaggaag gtattcttca
     2581 agatattgaa tatcagaaca acgaaggacc ttggcatgga atctgactaa tggaaagaat
     2641 gaaggagctg tgactttgtt gcggctcttt ttatttttat agagaatatt gtttctttca
     2701 gtagtttaac ccaggataaa aagtatgata gaataaagaa gcaattttta gcagaaaaga
     2761 ggcattttaa tgacggaaaa tttgaaccag acgatcattc agcttgtaca aaaagaactt
     2821 tcagactatc gtcctaagca gttgactact gtacttaatt tgttaaatga aggaaacact
     2881 gttcctttta tcgcgcgtta ccgaaaagaa atgactggaa gtcttgatga agtccagatt
     2941 cgtgaaatcg aagagcggta tgcctatctt gaaaacttag aaaaacgtaa aactgaagtg
     3001 attcggctaa tcgatgaaca agggaaactg actcccgagt tagaagcaga aatcacccaa
     3061 gccgtgaaaa tgcaacaagt ggaagatttg tatcgtccat acaaacaaaa acgacggaca
     3121 aaagcaacga ttgctaaaga aaaaggactt gaaccactcg cgaagtggct gatgcagctt
     3181 acagatggag aggtgcaatc tgaagcagaa aaatatattg acaaagaaaa agaagtctct
     3241 tctgccgaag aagcgttgca cggtgctcat gagattattg ctgaacaagt cagcgataat
     3301 gcaaaatttc ggacttggat tcggtcttat acgtataaca aaggtatgta tgtcagcaat
     3361 gtcaaggatg agcaagcaga tgaaaaaggc gtatatgaaa tgtattatga ctttgcagaa
     3421 cccgtccata aaatggtttc tcatcgaata ttagctacta atcgtggaga aaaagaagat
     3481 attctgaaag tattctttca agtagatgaa gcagcaattt tagcttactt ggatcgccaa
     3541 atagtcaaaa atcctgcctc tccttcttct tcattcgtca gagaagccta tcaggatagt
     3601 tataaacgct ttattcagcc agcaatcgag cgagaactgc gcaatgaatt gactgaaaag
     3661 gcagatgagc aagcaatcgc tattttcggc gaaaacctcc gtaatctttt gttgcaaccg
     3721 ccactaaaag gtaaagttgt cctgggattt gatccggctt atcgtactgg atgtaaacta
     3781 gcagtcgtag atgcgacagg gaaagtttta gcaatcgaag ttatttatcc tcataaacct
     3841 gcagcgcaag caaaaagaga agctgctgga ccagctttta tccaattaat taataaatat
     3901 caagtagata tggtagctat cggaaatggt acagccagtc gggaatctga actttttgtg
     3961 gctgagcagt tgaaatccgc cgaccataaa acctattacg ctatcgtcaa cgaagcaggc
     4021 gcttctgttt actccgctag cgaaattgca cgaaaagaat ttccgcatct acaagttgaa
     4081 gaaagaagtg ctgtttctat tgcccgtcgc ctgcaagatc ctttagctga attagtcaaa
     4141 atcgatccta aagctgtcgg agtaggccag taccaacatg acgtttctca aaaacgtttg
     4201 gctgaacaat tggattttgt tgtggaaaca gcggttaacc aagtaggtgt ggatgtgaat
     4261 acagcaagcc cgcagctctt gcagcatata tcaggattga ataaaacaac tgcccaaaac
     4321 attgtcattt atcgtgaaga aaacggagaa ttcaccgcac gaactcaatt gaaaaaagtg
     4381 ccacgcttag gaccaaaagc atatgaacaa gcaatcggat tcttgcgagt ccctggtggg
     4441 aaaaacattt tggacaacac aggtatccat cccgaaagtt attctattgc aaaagaaata
     4501 ttaacttcag tccaactttc agaaaaagag cttggtacag aagaagcagt agaaaaattg
     4561 acacgattat ccgtagagaa actggctgaa agcctatcag tcggagaaga aacgttagcc
     4621 gatattttag ctgggctgac acaacctgga cgagatatgc gtgatgagat gccagcgccc
     4681 ttattacgta cagatgtatt gagtatggaa gacttgaaac cagggatgga attgactgga
     4741 acggtccgta atgtcattga cttcggcgca tttgttgata tcggtgtgaa gcaagacgga
     4801 cttgtccata tatcgaaact cagcaaaaaa ttcgtcaagc atcctacaga tgtcgtgtca
     4861 gtaggagata tcgtaacagt ttggatagaa caagtcgata cgaaaaaagg cagaatcagt
     4921 ttgacgatgc tttcccctta tgaagagtaa caatgaggag taacaaaaat tgacacaaga
     4981 agaactgcag cagttagtag aagagatatc attaaaaagc ttccagcttc ctttcaagca
     5041 ccaagcaatg ttcaatccac gcttacgtac aactggcggg cgctatcatt taaacgatca
     5101 tcatttagat tttaatgcga agctatttga agagtcagac gaagagacga ttgcaggaat
     5161 catcaaacat gaactttgcc attatcatct tcatctagct ggaagagggt atcagcataa
     5221 agatatggat ttcaagtatc ttttgaaaaa aacaggaggg ttgcgctatg caccagcctt
     5281 gacgaagaaa cagataaaga tggagtatta tcgatgccaa gattgttctg ccacgatctg
     5341 ccgcaagaga agaatcgatg tacgcaaata tcgttgtgga agatgtcatg gagaaatcaa
     5401 atggattgag acaaaaagag aagactttaa tgaagcacaa aaaatcagat gaaaggatag
     5461 aaggcctttg gaaaaaagtg agcagaggaa tctaaaagac tggaatcagc tattaaattt
     5521 tttagccgca cctgaagagg gaaattttga ggaactgacg gcacctacac ttattttagc
     5581 tggaaactgt cttcctatct tagctgataa agcagcacaa atgtatttaa atggccaagt
     5641 agatcaattg tttttggtcg gaggggtggg gcatgcgact agaatccttt atgagaattt
     5701 tgaaaagcaa ggatttcatt ttgaagatgg catgagtgaa agtgaaatct gtcgtcagta
     5761 tctaaaagaa gtctatgatt tgcccgataa ggcttttctg atagaatcga agtctacaaa
     5821 ttctggagag aacgccactt tttcactgga gattcttcat tccttaggag ctgtaccaga
     5881 aaaaatctta ttgatgaatg atccgacact gcaacgcagg actcgagcaa cttttgagaa
     5941 agtatggcaa aatgaacaaa ctattttcgc gaatgctgtg ccatttgtgc cggaaatcct
     6001 tcacttttct gaagagatca tctttacagc aaaagaactg aatcatcaat ggcctaagga
     6061 atatttccat gcgctagtat taggagaaat ggaacgtctc catgacgatg agaacggcta
     6121 tggtccgaaa ggaaaagatt ttattcctca cattgatata tcagaggaag tttggtcttc
     6181 ttacaccaga attaaagagt caataaaaac ggatttttct cgtacttgac cattttagca
     6241 agcgaatcct cctttccaaa taaatttggt tgaaattttc aatcgtttat aagataatga
     6301 ggagcgtgtt gaatgtaata gctaaaagaa tggacgctag ataagagaag aggattcaaa
     6361 tggaaaatca aaataaattg tatcattttg ttggtatcaa aggttcagga atgagctcct
     6421 tggctcttgt tttacatgaa caaggtctaa atgtccaagg atcagacatt gaaaaatatt
     6481 tctttacaca aagagattta gaaaaagcaa atatcacgat tctgccattt aacgcagata
     6541 atgtaaaacc agggatgacg atcattgcag gaaatgcatt tcctgattca catgaagaaa
     6601 tccagcgagc aaaagaatta ggtttggaag tgattcgcta tcacgacttt attggtcatt
     6661 ttatccaaaa ttatacgagt atcgctgtaa caggctctca cggaaaaaca agtacgactg
     6721 gactgctctc tcatgtattg agtggtgtcc gtccaacaag ttatctaatt ggagatggaa
     6781 caggacatgg tgatccgcaa gcggaattct tttcatttga ggcctgtgaa tatcgccgtc
     6841 atttccttgc ctattcacca gactatgcaa tcatgaccaa tatcgatttt gatcatccgg
     6901 attattatac aagtatcgac gatgtattta cagctttcca aacaatggct ggacaagtga
     6961 aaaaagctat ttttgcgtat ggtgacgatg cttatttacg caaattaaaa gcaaatgtgc
     7021 cgatttatta ttatggtgtc acagaaaacg atgatatcca agcacgaaac atcgaacgca
     7081 caacttctgg ttctgctttt gatgtctatc atggggatga atttgttggg cacttcactg
     7141 taccagcatt cggtaaacac aatatcttaa atgctttagg tgttattgca gtagcttatt
     7201 tcgaaaaact ggatctgaaa gaagttgcag aagaaatgct gactttccca ggagtaaaac
     7261 gtcgattcag tgaaaagatc gtagcagaca tgaccgtagt cgatgattat gcgcatcacc
     7321 ctgcagagat caaagcaacg attgatggcg cacgccaaaa atatccagat aaagaaataa
     7381 ttgcagtttt ccaaccccat acgtttacgc gtacgattgc attgatggat gaatttgcag
     7441 aagcacttga tttagctgat aaagtttact tatgcgatat ttttggttcg gctcgggaag
     7501 aacaaggaaa cgttaagatt gaagaccttg gagcgaaaat caaaaaaggt ggagaagtca
     7561 tcaaagaaaa taatgtatct ccattgttag attatcatga tgcagtagta atcttcatgg
     7621 gtgctggaga tgttcaaaaa tttgaacaag cgtatgaaaa acttttaagc agcacgacga
     7681 aaaatgtact gtaatacaat tactgcagtg tccttataaa aatagaaaga gggggagtgg
     7741 caaaaatctt gccaagcccc cttttttgca acttatg
//
LOCUS       DBNICI010000124         7759 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.125, whole genome shotgun sequence.
ACCESSION   DBNICI010000124 DBNICI010000000
VERSION     DBNICI010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.125"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            145..1092
                     /locus_tag="AC1ZCC_002185"
     CDS             145..1092
                     /locus_tag="AC1ZCC_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1630417.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            1104..2024
                     /locus_tag="AC1ZCC_002186"
     CDS             1104..2024
                     /locus_tag="AC1ZCC_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1630418.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NNWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            2065..3522
                     /locus_tag="AC1ZCC_002187"
     CDS             2065..3522
                     /locus_tag="AC1ZCC_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIQ1630419.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3867..4481
                     /locus_tag="AC1ZCC_002188"
     CDS             3867..4481
                     /locus_tag="AC1ZCC_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HIQ1630420.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4474..6198
                     /locus_tag="AC1ZCC_002189"
     CDS             4474..6198
                     /locus_tag="AC1ZCC_002189"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIQ1630421.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            6195..7646
                     /locus_tag="AC1ZCC_002190"
     CDS             6195..7646
                     /locus_tag="AC1ZCC_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIQ1630422.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2620 a         1289 c         1541 g         2309 t
ORIGIN      
        1 gtttttttct ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt
       61 tcattttccc tatactttgt ttatttattc tcattattga aagcgctata attacgttgc
      121 gaatgaatcg aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg
      181 aatctatggc gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt
      241 tttttctttt acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca
      301 gcagatgggt tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa
      361 tttttatttt cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta
      421 gcatttatcc ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg
      481 agaaataaac gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct
      541 tgggtcatta tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat
      601 cagtggatca ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg
      661 ccgtttatct tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac
      721 tttgcatctg ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct
      781 agcaaatggc agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt
      841 ttaacgattt tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt
      901 cctaggaatt ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat
      961 ggcctgacat ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt
     1021 gtcggctttg tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct
     1081 gcattattct aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct
     1141 ctttcaataa gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct
     1201 gtattttgcc atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga
     1261 ctcacgggta cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg
     1321 accagatgca ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag
     1381 gtacactgat caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact
     1441 ttccattccg tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga
     1501 tggtggccaa ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat
     1561 tgatcttgcc aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac
     1621 gttcggtctc tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa
     1681 tatttgttca gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg
     1741 ccgcattggg ttactggaat aactggttca atgctttact atacatccaa gatgataagc
     1801 tagttccact acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa
     1861 attcaggagc aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca
     1921 gaatggcgat cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga
     1981 agcattttgt caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa
     2041 atcataaaat taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt
     2101 tcagcgttgt tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca
     2161 gaagctagca attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc
     2221 gatcaattga tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat
     2281 atcaattaca ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg
     2341 gaaaattatg atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt
     2401 gctgatttga ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa
     2461 gcatatatta aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat
     2521 gtctttgctc aacaagtact tacttttaac aaagctttac tagataaata cgatctttca
     2581 attgatggaa tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa
     2641 gaaccaaaca cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat
     2701 tatcctttag ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc
     2761 atcaaccaat acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac
     2821 aaacaaggac tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga
     2881 aatacttggc taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg
     2941 acaaatgcag caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca
     3001 gctcaagcac aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca
     3061 gtagaacttc tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg
     3121 atcgagggag aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat
     3181 ggttatcagc caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat
     3241 acacaagagt ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca
     3301 gaaacttcac cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt
     3361 aatatttcta atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca
     3421 gatgaaacat taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg
     3481 aaagaaatgc aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag
     3541 gggttgtgac aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca
     3601 gtttctcggt attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt
     3661 tcttactact cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag
     3721 ctaaccctcg gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt
     3781 tctctaataa tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta
     3841 taaacttaat gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc
     3901 ggatctgggt cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga
     3961 tcatctttgg attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat
     4021 tcgagtataa agaaatcaca ataaaaagag gctggaatat atataaaaga aattttttaa
     4081 gaggcaatct gctatttagt ttatttttat ctggtactgc tttattgagt tacaatttat
     4141 atttatctgt tcaaatcaaa gggctcattt ttttagctat tgattttatt ttactttttg
     4201 ccttgctctg cctatttgct acttatcaat acagtatgat tttagatagt gaatatacga
     4261 tctctgttcc taatctgcta aagctaagtt ttatttcggt tttttccagc ttttcgtctt
     4321 ttctaaaaat aattattggt agcggtataa ttttaggcgt gacttggcaa ttcaaaggat
     4381 tgatcctgtt tggtgtcatt ggtcttttga cggtttggaa tggtacgatg acaacacatt
     4441 ggcgtgaaga attagataaa cagttggaaa gttatgaata agtttcggat tctattttca
     4501 aaaggaacct tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat cggtgtaacg
     4561 atgactgcta ccgttatttt tagtatccat acttgggaac aaaatcaaaa acaggcagaa
     4621 aacatgacaa gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa aacaaccttg
     4681 agtaggatca tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa tgtgacgacc
     4741 tacttaacaa aacccataga tcagtatctg atgtatgtat acgaacagca aaacagtacc
     4801 gatgaattag tttctttccc aaatcaaatc aaagatctct acgcaaatta tgaagaacta
     4861 agtgcgatat atatcgtgtt gaatcaacta ccggagtatt atgaatcttc tagagaaaac
     4921 aaaggtgggg aaataaaaaa ggggacacca aaattactag acgcctttta tatcaagttg
     4981 cctatcacac aaggaggagc tgagtcagga agtattttta ttggttttca aaagaaagaa
     5041 ctagatatga ttttagaaaa tctaacgtca ttcaatggac tctctttata catgatttct
     5101 ggaacgacaa atcgactata cacattccat gataataaaa tagctaaaga aacgtttgaa
     5161 agacaagaaa tgatcatcac agagagtctg aagaaaaatt cacaattgcc gattgacgat
     5221 ctggaaaaaa acaattttgt tcaacaccaa gaactgtctg atgattatcg tatattagct
     5281 gtactagata aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc gctgatcatt
     5341 ggtatcatta ttttagacaa tatactgctt atctttcttt ataaaacatt caaacgatat
     5401 tcacagcaag tatctatcgt catgcaggcc atggatcaaa cagcagcagg gaatttagag
     5461 acgcggatcg acataacaaa cacggaatat gaactgaagg aactttcaat cggtatcaac
     5521 gaaatgttgg atagcatcaa tcagtttgta gaagacatct acaaattaga aatcaaacag
     5581 caagacgcac atatgcgggc gcttcaagca cagatcaatc cacacttttt atataataca
     5641 ttagaatata tccgaatgta tgcaatcagc gaaggaagtg aagaattagc agatgttgtg
     5701 tatgcgtttt ccgctttgct tcgaaacaat acgaatcaag agaaaacgat cactttaaaa
     5761 gaagaactgg acttttgcga aaaatatgtc tatttgtatc aaatgcgtta tcctaatcga
     5821 gtagcctatc attttatgat cgatcctgat ttggaaaaga tcgaagtacc taaatttgtg
     5881 atccagccat tagttgaaaa ttattttaaa cacggtattg attttactcg attcgataat
     5941 gcgctaagtg tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat caaagataat
     6001 ggaaaaggga tgactgaaaa acggctaaaa cagattgaag aaaaactgtc tcatcctaaa
     6061 gtggaacttc acggttctat cggtctgcaa aacgtgaatg aacgtttacg tgccagcttc
     6121 ggcagcagtt attatatgtc gcttgagaat aatgagacag gtggactgac cgtttctatt
     6181 acattcaagg agggatgagc gtgtatcaag tgttattggt agatgatgaa tacatgattt
     6241 tgaatgggtt aaaaaagata attgattggc aaagtttagg ctttcaaata gtagcaacag
     6301 cagagaatgc caaagaagga ctagctgtac ttgaacagcg acagattgat ttagtagtaa
     6361 cggatgtgac aatgccagaa ataaatggac tggaatttat tgaagcggct caaaaagaac
     6421 gtcataattt tgaatttatg attttatcag gataccaaga attcgattat ttgaaaggag
     6481 gtatgcaatt aggcgccgtg aattatttga tgaaacccgt gaataagttc gaattggttg
     6541 aaagcctcaa gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa aatcaacaag
     6601 aaatctatca agaactgtta ttcagtcaat ggttgaatga agaattggat gaaataagtg
     6661 aagaagaaat aataggacaa ataagtgaga agcagcatag gatagtattg attcaactgg
     6721 caagaactca tgatactctt atcaatagat ggttgcaaga acggcaacaa atcttttact
     6781 atcaacgaaa ttacggagac cgtgtattga tcacgttgtt gttggaggca gatgaagttg
     6841 atggcttctg ttgttttgtt caagaaaact ttcctgatca ggaatggctg atcagtatcg
     6901 gggaagagac agattctttg gacaaaatcc ctgaaacatt ccaacaagcg aaagacaatc
     6961 tacagttgca tcaattttat ggagataaag acgagcatgt tttttacgca gtacaagcag
     7021 aaacaaaaga tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa aataaacaat
     7081 tagaagtcgc tcaaaaaatg atcactgatt tttttgaaca gtttcagtta gcagccatga
     7141 tgccggaaga tatacgatat tccagttttt tactgtttat ggagattcaa cgggaattga
     7201 ttgatctgga agatgaggaa tatttacaag gaatcgaaaa aatccatcag gcaaaaacgg
     7261 tgcaagaact tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga cagaaaaagt
     7321 acagtgacaa tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa gaaccactta
     7381 cactcaaaga agtgtcagaa aacctgcatt taaatgtcat gtatctagga cagctgttca
     7441 aaaaagaaac gaaaaaaagt ttttcagctt atttgaacca tttacgtatg gaaaaagcga
     7501 aacaactgct tttgcacagt aatcaaaaca tcaatgagat agcaagtgag attggctaca
     7561 acaacaccac gtatttctct aaattattca agaaaatcgt cggcagatcc cctaaagagt
     7621 accgagaaaa cagtggagaa tcataaaaga aaaagccaat gtaatgaaat aacttataaa
     7681 aaattgtcta gaaatctaat tttgggctgt tcttattact tgaggcaagg cagctttttc
     7741 atgacctctc gataaacgg
//
LOCUS       DBNICI010000125         7710 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.126, whole genome shotgun sequence.
ACCESSION   DBNICI010000125 DBNICI010000000
VERSION     DBNICI010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.126"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(169..666)
                     /locus_tag="AC1ZCC_002191"
     CDS             complement(169..666)
                     /locus_tag="AC1ZCC_002191"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HIQ1630423.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(706..1515)
                     /locus_tag="AC1ZCC_002192"
     CDS             complement(706..1515)
                     /locus_tag="AC1ZCC_002192"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIQ1630424.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            1849..2706
                     /locus_tag="AC1ZCC_002193"
     CDS             1849..2706
                     /locus_tag="AC1ZCC_002193"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HIQ1630425.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2699..3601
                     /locus_tag="AC1ZCC_002194"
     CDS             2699..3601
                     /locus_tag="AC1ZCC_002194"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HIQ1630426.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3634..3984
                     /locus_tag="AC1ZCC_002195"
     CDS             3634..3984
                     /locus_tag="AC1ZCC_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HIQ1630427.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4033..4955
                     /locus_tag="AC1ZCC_002196"
                     /pseudo
     CDS             4033..4955
                     /locus_tag="AC1ZCC_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
     gene            5033..5926
                     /locus_tag="AC1ZCC_002197"
     CDS             5033..5926
                     /locus_tag="AC1ZCC_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIQ1630428.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            5964..6527
                     /gene="amaP"
                     /locus_tag="AC1ZCC_002198"
     CDS             5964..6527
                     /gene="amaP"
                     /locus_tag="AC1ZCC_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HIQ1630429.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6541..6729
                     /locus_tag="AC1ZCC_002199"
     CDS             6541..6729
                     /locus_tag="AC1ZCC_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HIQ1630430.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            6742..7644
                     /locus_tag="AC1ZCC_002200"
     CDS             6742..7644
                     /locus_tag="AC1ZCC_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIQ1630431.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
BASE COUNT         2691 a         1353 c         1498 g         2168 t
ORIGIN      
        1 gagggtcagc tgattgaaca ccatttaaga aagaggtcgt gagcctgccg tttcactctg
       61 tgtcataaaa aagccaatct actttgatag aaaaagcacg taggactaaa acagttctgc
      121 gtgctttttt tattgtgtgt taaatatcgt aaatgaagat agatattttt atgattcgac
      181 gatagagacg ttgaaatctt tcaacagctc taaagcggat tctccgtgtt ttctactgga
      241 gactaacatt ggatcaacaa aaaagtctgc ttcaggaaaa gcactctgca ggcctatcag
      301 attagagagt acacacaagt ttgtttcaat cccgactact tcgatctctt tttgttcttc
      361 tttgctttca aataaagttt cctggatttc agctaaagtt tcaggtggaa tcgtaaagta
      421 atacttttta atcactcgtt cattttcttg tggagaaaga agagggatca attgcaaatc
      481 ttctcgttct tcttctttgt ccttcagttc aactggtata tcccgtgtga agagaatata
      541 ttcattattt tggcgtgctt tagctaatct ttgagcaata cgttctgcca attgatctga
      601 ctctggaata taaaattcgc tgtttggatc aagaatatga ttttgcatat caataataac
      661 taacattttc tttcctccta cattcatatc gtcattttat tctttttaat agaagttttt
      721 taaaatataa gcaacaccag cttcttcatt cgtcagtgtt acttggtttg caatttcttt
      781 tacttgtcgc tgtgcattcc ccatcgcaat actcaacccg ctgtaattca acatagcaat
      841 gtcgttttct tgatcgccaa aagccatgat gtcactcgat tcgattccaa ggtaatccgc
      901 cagaaaaaca agtgaatgag ctttagtgat acctgtaggc gtgatttcta agaaatgcgg
      961 atcagagaat actgattgga taccatctgc ttgcagatct ctttgtagct tctgtagttt
     1021 ttcttcactc ttatctgtta atgctaattt aagatagcta agagaaggat cttccaacat
     1081 atttaaaatt tgatcaaaag aatggttctg taaattgaaa tgttcataat aagggtcatc
     1141 tttgactaga tttgattcat cgaatgtcag ctgatcatct tgggtttctc ggctagcaat
     1201 gatggatatt ttcgcttctt gagcatagaa gaaggctttt ctcagtgtat cgctatctat
     1261 ttttgaacgt ctgagaactt tcgccatttt tccatccttc atttcagcaa tcagtgaacc
     1321 attggaagaa atcaaatagc caccgattcc taattcggaa aaaatatctt tagcagaaat
     1381 gctgttcctt ccggtagcga cggttacgat tccgccattt tcatcaaatt tccgaagtgc
     1441 ttggatattt tcttctggta tctttccatt tcgatcaagc aaggtaccgt ctaaatcaat
     1501 cgctgctagt ttcattttat cttcctactt tctttttaca tatctatttt cctgttacct
     1561 taagattaaa tgagaattta aaaaaatgaa actaacttgc tttgtcatta tgaatttttt
     1621 caaaaaacat tcttaagaaa aaggtggcta tatatagtag tttttggtca atacctttga
     1681 ataaaaagcc atgaaaaacg aatagttagc gcttaccgat gcgttttgtg ataaaaaaat
     1741 tttaaaatat tcaatgaaaa caaagcatat attttgcttt aaaaccaaat gaggaatatt
     1801 ctagatgtag aacaggttag aaaaagatta gaaaaaggag tgattttgat ggagaagaga
     1861 tacggcgtag ttcctgtggt aactgcttca gacgaaaatt atgctcctta cttgagcgtg
     1921 atgatcgcaa ctgcattaga aaattgcaat aaaacaagac gcataaaatt ttatgtgatt
     1981 gacgatggtc tatcagaata tagtaaagaa ggattggaag aaacggtaaa taaatattca
     2041 agtaatgcaa gtatccaatt cctgacagtg gaaaaagata tctacgaaga cttcctagtc
     2101 agtgaccata tcacaactac cgcttactta agaatttctt taccaaactt attagctaaa
     2161 gaggattata agaaagtcct ttacttagat tcagatgtgt tagtactaga cgatatcgta
     2221 aaactatatg atgagccgtt aaatggaaaa acaatcggcg ctattatcga tccaggccaa
     2281 gtgaaagctt tggaaagatt aggaatcgat tccgatgact tgtacttcaa ttcaggagtg
     2341 atggtgattg acattgatca atggaataaa aaagaaatta cagaaaaaac tatccattac
     2401 ctcagtgaaa atggcgatcg aatcatctat cacgaccaag atgctttgaa tgctgtactt
     2461 tatgaagatt gggaacaact tcatccaaaa tggaacatgc agacttcgct gatttttgaa
     2521 cgtcatccag caccaaatga aaaatatgaa cgtcagtata aagaaggaaa tgaaaaacct
     2581 tcgatcgttc actttacagg acatgataaa ccttggaata cgttgaaaga tcatccgtat
     2641 accaatttat acttaaaaaa attagcacac agtactctaa cgaaagtagg cgagatcaat
     2701 gaataaaaaa gaaatagcag tagtcgcaag ttgtaacaca aaattcgtac cacatttagc
     2761 tgcattattt gtctcagtat tagataattg caacccgtct aaatttgttc ggttctatgt
     2821 gattgacgat gatatcgatt ttgaaagtaa acagctttta cgcttctcag tgaaaaacgc
     2881 acgaatgaac tcagatgtgg aattcttgaa aatcaataaa gaattcttta caaatgtggt
     2941 aatcagcgat cgtattccag aaacagctta ttatcgtatt gcgattccgg aattgttccg
     3001 cggcacagaa gttgaacgta tcttatatat ggattgcgac atgatcgctt tacaagatat
     3061 ctctaaatta tggcgtcttg acttcggtga ttcaatcgta gcagctgttg aagacgctgg
     3121 gttccatcaa cgactggaaa aaatgaaaat ttcagctaaa tcaatgcgtt atttcaactc
     3181 aggtttgatg ctgatcaatg tgaaaaaatg gttagatgaa aatattacgc aaaaagtatt
     3241 agatttcatc gaacataatc cagaaaaatt acgattccat gaccaagatg cattaaatgc
     3301 tatcttacat gaccgttggt taccacttca tccgcgttgg aatgcacaag gctatatcat
     3361 ggcaaaagca aaaaaacatc caacagcagc aggtgaacgt gaatacgaag aaactcgcaa
     3421 caatccttac attatccatt tttcaggaca tgtgaaaccg tggagcaagg attttgaagg
     3481 gccaacgaag aaatattacg aaaaatatgc agggatgacg gctttccgtt gtgtagctaa
     3541 attccctaaa tatccaaaat atgcaaaagt tcaaccaaaa acagatgtag ttgttaaata
     3601 atagaagacc acaactgaag agggggagta ggaatggaaa ttacaatgtc taaaaatgca
     3661 gtagagacgt taattgaaaa ggttggggaa aatacaaaaa tcgcactcgc gcttatcaat
     3721 gattccgatc catttctaag ggacaaaggt gcttttgcaa aaggcagctt ttttcaaatc
     3781 attccttttg tttcggaatt tggagaatat gcgactaaaa tcgagcatcc attattggac
     3841 atctatacat ctaaattaga gcaaaattat ttcggtaaac gattgaacat ggacttcaac
     3901 aaacagcttg acagcttttc attagaaaat gaaatcgcag tactcgatta taatatcaaa
     3961 ttaaagaact gcttcttcag ctaatagttt cgagaaagtc caaaaaggag gtatacattg
     4021 agatactact ttgtggatga agaaaagaat gtaatagaaa cagatgaaaa aaatataatt
     4081 ggctcgtact tgatagtgca aagcccgaag agattgagca agcaatcgaa gaattcgagc
     4141 ttcctgatga tattttcgta ggaaccgaat atccggaaga agtttctcgg atggagcgcc
     4201 tttatgagac gaaactagag catccatttt ctttggtagt gattaatcta gatcagtcag
     4261 ataatcgtat cgaaaaaaaa ttaacgccaa tatcttttgt actttccgat aatctcttga
     4321 ttacttgtat cgatagaaaa accgaatttg tggatcggct gctgacaaag catggaaatc
     4381 atttaaactc gtttgaaaag atcatcacgt atacattatt agatatttac acccattacg
     4441 tgaaagaact aagagagatg aaaaagcgaa ttgacgcttt ggataaagca gctcgtaaaa
     4501 caacggaaaa tgaagaactg tataaacagg cagatcttga aagagacatc gtatatatcg
     4561 atcatacctt aagagatcaa cgggaaacaa tggatcaatt atggaattct gaaactttca
     4621 tcaaacgttt agacgatgaa cagctactct atgatgtcca tttgagacag cgacaaacag
     4681 aaaaaatgat cgagatctat cgagatttat tagagagtat tggtgatcta ttcaacggga
     4741 tgatggacaa taatctaaat catatcatga aatatctaga ttcagctgca ttaatcattt
     4801 ccgttccggc gttgttcgca gggatttggg gaatgaatac aggaggatta ccgggtaaaa
     4861 gttctgctct aggctttata ctcgtagtct ttggctcttt gatagcagca gtagttttag
     4921 gttaccattt ataccggaaa gattatacaa attaaagaat ctatttaaaa caggtatcaa
     4981 acaaagaaat ttgttttggc aatacaattt aaaaactagg aggaatacag caatgacaga
     5041 accaaaatta atcgacccgc gaaacttgta ccatacggag aaatttccta aacaagatca
     5101 ggatacacca gctttacagc agaatatgat tcctgttcct gattgtggag aagaatccta
     5161 tgaaggcaat aatcaactag aaaaccgccg tgtactaatc actggtggag attcagggat
     5221 cggtcgtgca gcagcgattg catttgctag agaaggggca gatatcgctc ttcaattctt
     5281 cccaggagaa gaaaaagatg cagaggaagt agcaaaatac gtgaaagatg caggacgtaa
     5341 gattgtctta ctaccttttg atcttcgtga cgaacaagca cctaaagaaa tcatagataa
     5401 agccgtgagt gaacttggcg ggctagatac gatggtacta aacgcaggac agcaaatttc
     5461 ttgtcagtct attgctgagt taccacttga gcaagtcaaa gacacattta tgataaacat
     5521 catcgctatg tttgctttag tgaaagaagc tgttccgcat atcccagcag gaggagcaat
     5581 cgtaacaaca acttctgttc aagcattcaa tccaagcgaa catttattag attacgcagc
     5641 aacaaaagct tcaatcgcta actttacgat cggtttagca aaacagcttg cacctaaagg
     5701 aatccgtgta aatggtgtag cacctggtcc aatctggaca ccattacaac tagacgaagg
     5761 acaaccttct gattcgatcc ctgaatttgg tcagcaaagt ttactagaac gtgcaggtca
     5821 gccaggagaa ttagcaccag tttatgtttt cttagcatcg aataaagcaa gttatgttac
     5881 agcccaaatt tacggagtaa ctggtggaga agcgatcaac ttataagaag ttgatcgttt
     5941 cacccctatg aggaggtgcg aaaatgaata aaggtgtaaa aacgatcgga gtgatcggaa
     6001 cattactttt gctatctgtg cttttgtttg cgatactatt aagtgcaacc tatccattgc
     6061 cttttcagct agagaagttt cgattctttt ccatcactaa ttcatacgtg caacaatatg
     6121 tattttggct tgccgttgca tttgctattt tgacaattat cacgttactt gttttcctgt
     6181 tttatccaaa aacaatcgga aactttctac tgaaacatgg tgatggggag ttgacgttgg
     6241 ataaaaaagc aattgaagga atggttcgtt ctcacttgca tgaagatgaa ttcatccatt
     6301 caccaaaagt aaatgtcaaa gcaacgaaaa acaagatcgc cgtcaatgta aaaggggagt
     6361 tgaaacgaac ttcagcactc attggtaaaa caggaacatt gatgaaagaa atcgaaaatg
     6421 aagtacaaca catattggga acaaaagagc cagtgaaagt atcggtgaaa tacacaggct
     6481 ttgaagaacc agctaaacga agcaatgacc gcaaacgagt agaatagggg gggaccaaaa
     6541 atgaaagaac tattttcgac atacaagctc cctatcatct ttggggtact tggattgata
     6601 ttagccgttt tatggatcac cgtgggcttt ttcaagacct tattgatctt gatcatggca
     6661 gcgatcggcg tcgctatcgg tttttatctg aaacaaacga aaatcttaga tcactatttt
     6721 aaatcttagg aggaaacatc catgaataac aataacaaaa ataataacaa taaaatgaac
     6781 aaccaaaaac cagtgtctgc acatccagac ccaacgacac caaaccaatc aaatggtgtg
     6841 aacgatgaaa agaacaaaac agcaccagta tctgcacatc cagatccagt agtccctaac
     6901 gttcaacaag gagtagaaga gaagaaacat gatgcagaaa agcatgcacc tcatcatcca
     6961 ggaactccag aatctaaagt tccagaacaa catgatgcaa tcgatccttc taaacctgtg
     7021 gaagagcaaa aacatccttc tgaccatgcg aaagcacaag aaaaacttag ttctgaccac
     7081 acaaacggac aaggaaaact tggttctgat catacaaata accaaggaaa gcatgattct
     7141 gaacaggaag attcaatgcg cggtgagctg aattatgaag ataaagttgt gcaaaaaatc
     7201 atcggaatcg caattgaaca agtagacggt ttattatctg caaacggtgg tttcttctct
     7261 aatgtggcag gaaaacttgt gaatacagat aatgtaacgg ctgggatcga aacagaagtc
     7321 ggtaaaaaac aagtagcagt tgatatggat gtcattgtgg aatatggcaa agatatcgaa
     7381 aaaatctttg aagaaatgca agaagtaatc ggcaaagaag taaaaaatat gacacattta
     7441 gaagtgatcg aagtcaacgc taacgtagtg gacatcaaaa caaaagaaga atttgaaaaa
     7501 gatcaagaaa cggtgcaaga caaagtaact gacgcagcga aaaaaaccgg tgaatttgct
     7561 tcgaatcaaa cagataaagt aaaatcagca gctggaagcg gcgcacaaaa agtaaaagaa
     7621 aatacagaac cacgggttca gtaataagga agtaaaacat taggaggaac gaaacatggg
     7681 atttatttgg tcacttatcg tcggaggaat
//
LOCUS       DBNICI010000126         7684 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.127, whole genome shotgun sequence.
ACCESSION   DBNICI010000126 DBNICI010000000
VERSION     DBNICI010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.127"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..645)
                     /locus_tag="AC1ZCC_002201"
     CDS             complement(<1..645)
                     /locus_tag="AC1ZCC_002201"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIQ1630432.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFK"
     gene            complement(645..1781)
                     /locus_tag="AC1ZCC_002202"
     CDS             complement(645..1781)
                     /locus_tag="AC1ZCC_002202"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIQ1630433.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDIVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(1813..3264)
                     /locus_tag="AC1ZCC_002203"
     CDS             complement(1813..3264)
                     /locus_tag="AC1ZCC_002203"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIQ1630434.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(3308..4072)
                     /locus_tag="AC1ZCC_002204"
     CDS             complement(3308..4072)
                     /locus_tag="AC1ZCC_002204"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HIQ1630435.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(4100..4798)
                     /locus_tag="AC1ZCC_002205"
     CDS             complement(4100..4798)
                     /locus_tag="AC1ZCC_002205"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HIQ1630436.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(4810..5589)
                     /locus_tag="AC1ZCC_002206"
     CDS             complement(4810..5589)
                     /locus_tag="AC1ZCC_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0061097 - regulation of protein tyrosine
                     kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YveK family protein"
                     /protein_id="HIQ1630437.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(5605..6549)
                     /locus_tag="AC1ZCC_002207"
     CDS             complement(5605..6549)
                     /locus_tag="AC1ZCC_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIQ1630438.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(6595..7374)
                     /locus_tag="AC1ZCC_002208"
     CDS             complement(6595..7374)
                     /locus_tag="AC1ZCC_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630439.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         2174 a         1617 c         1173 g         2720 t
ORIGIN      
        1 tttaaaaatt tcaatcaaaa aacgatgatt tttagaatat gaaattcttc caatatgtcc
       61 cattacaaac gtctgacttg aaattcctaa tttcattcgt acatcatttc taatactttc
      121 atcataataa aattttttag catccactgc atttggaatc aaagtgaatt tatcatttcg
      181 aaacaaaaaa tcacctgcta cttttccaca tgaaaattca tgtgtcaaaa cagacgttaa
      241 cttacatcga taatattgct ttaaaagata tttaaaatct ctatctatcg atgtattatg
      301 gctatgtgct atccgtatcg gaatcccagc tttttttgca gcttttaaag gtaaataact
      361 catcgcatca atatgagaat gaacaatctc atattctgga tgatccttaa aaaattgttc
      421 catccattta aaatatttag gataattttg tggataaagt ctcggtgcat aatagacctt
      481 tcctccaaga cttattattt cctcatcata atcactttta tatggacgat gtgtcaaaaa
      541 atcaaactga attttagttc gatctatatt cctataatag ttcattagca tcgtctccaa
      601 ccctgcacga tgcatatcat tcacacactg taatattcga atcattatat atttccccta
      661 tacaaattta atgtttcttg tacgatttta tcccaattat atttactaca aataaactca
      721 gtagcttgat tcttcaaaac tttaaccatt tccgattgat tgcatgcctc ttctaatctt
      781 atctttaaat cacttacatc cgatttctta aaaatcatag ccttgtcttc tactacttcc
      841 gtacactctg agatatttga aactatacaa cagttcccat aactcattgc ttctaacaaa
      901 cttaatggca ttccttctaa atcacttgga agtgtataga tatacgcatt actatataat
      961 tcttctaatt cctgtccttg tacaaatcca gtaaatataa ttcgctcatc accttttgct
     1021 aattccttta gttcatttgc aaattcatct gtatcagagc ttcctccggc tattataagt
     1081 tttttatctg tctgaacatc tttaaatgcc tcaattagat accgaattcc tttttcggga
     1141 accaatcgcc ccaaaaacaa aatataagaa tcttttgtta atccatactt ttctgtaatc
     1201 aatcctgctg attttatttc aattttttta acgccatttg gtataaacct tgtttttctt
     1261 ccatatatat cttcaaaata attttggacg ctttcactaa gtacaattat ttcatctgca
     1321 aattttacag cacacttttc ccctaacatg atgtatgtac tagcaagctt gttccatttt
     1381 tctctttgat gatctagacc atgaattgtt gcaacacatc ttttcccaaa aagttttggc
     1441 aaccacaaca tagcacatgg tccttctgca tgaaaatgaa caatatcata ttttccgaat
     1501 gctgctctaa ttccaccaaa tactgaagaa ctcattgctg caattccttt tatatttaat
     1561 gtaaaaatag tctttaattt tacaccttta tattctttat ttttatgctg gtcaaattcc
     1621 tttccgctaa catgatgacc acttcgatta tagcaagtca cttcatgccc aagtgcaacc
     1681 attcgcgttg ccaattcttc taccactatc tcaattccac cttctcttga tggaattctt
     1741 ttatgaccta gcatacagat ttttaatttt tttcttgcca tcacgtttta ctccttctat
     1801 agtatcttta tcttacatgg aaccgtttct atgaagcaca gttttgactg tcctaaacaa
     1861 aattctaaaa tctaatccca tactccaatt cctgatatat tctgcatcaa gttttacaac
     1921 ctcttcgaag tctgtgatat cacttctacc actcacctgc cacattccag taatcccagg
     1981 tttcatcgca attctcgcac gatgatgaaa atcatacttc tcatattctg acttcaagca
     2041 aggtctaaac cccaccaggc tcatttttcc cattaggaca ttataaaatt gtggaaactc
     2101 atccaatgat gtagatcgaa taaattgtcc aattcctgtc ttctttgttc catcaggaag
     2161 tattttattg ccaatgattc ttgggtcaaa atccatctta aacatgagat tattactcat
     2221 cttattttct ttcatcaact ctgctttgcg ttcttctgca tccatataca tactacggaa
     2281 cttatacatc ttaaatgtct taccattttg accaatacgt tcttgtgcaa agaaaatcgg
     2341 ccctggtgat ttgatgtaaa taactggtgc tacgaagata aaaataacac ctgttgcaat
     2401 acatccaata attccagcaa gaatgtcaat cgttctcttt ataaatgctt gcctcatcgt
     2461 cataacatta atactagatg taataacagt ataatcacca actcggccaa taaattgttt
     2521 tccacgtgta ctatcagaca cttttgtaag attcatatgt acagcaagtc ccatttccga
     2581 acatctggcg attaattctt tgggatatgg aacactttcg tcaacattga tgaatacttc
     2641 atctacccaa ttctgtaaaa gatattccgc tgcattatca gcatttgcaa ctactgggac
     2701 acctccgatg gtagttccat acatatctct gtctactaca atcacaccac taagtttata
     2761 catgccataa ttcttctctt taagattacg aactacattt tttgcaatct tttgagatgt
     2821 tacaataact aacgatacat cttcaccgtt tttcatgcgg tgatgcaaca accctttcca
     2881 aagtatacga attatatatg ttaataatcc atacaccacc cccattataa ataaagaaac
     2941 tctggaataa tcatttccat cctgcattgt aataaggtat aaactgcaca caagtaacag
     3001 cataaaagat tgctttatag aagctacagc ctctttataa tatcctcttt ttaaaacatt
     3061 tttatatgcc tcaaataaaa atataatcgt aagattctca agttctataa aaattgccat
     3121 gtggcgataa ataggaatca catacggatt ccagtcaccc tgtcgcatta catatgatat
     3181 ataaaaagca agctggacac aaaataagtc taataaaata aaatcaatat gtttagacca
     3241 accacttgag ttttttctat acatctctca ctctctcctc atttcctttt tatctacgaa
     3301 taaagaacta aaataaacca aacttcttct tttttacctc ttcatagatt ggataattga
     3361 tctcatcccc gttgatcaga tcctttgcga cttgtttcat ctgtactact ttttccttac
     3421 caaactcctt ggcaatctgc tcatagcact cttttaagta aaaggtgcgc ttagagaccc
     3481 cgtgtgcatc agaagcagcc atctgcacga gattatgttt gaccattgtt ttggctgttt
     3541 tctggatatc tttgccaaat acaccaacat aacttggcgc agtaagttgg gccaaacatc
     3601 ccatatctag gaacggaagc aaatgatttg ggtcttctcg aaaatgcgcg ttgcgttctg
     3661 gatggacgat cactggtatc tttcctagtt ctaacaactc aaaaaaaagc cgttctgcat
     3721 ataacggcac ttccagtgtc ggaaattcga tcagaagata tgtatcatct aaatctgtaa
     3781 ataaaatttc atctcggtgg atgtcttcga tgagcgttcc ggtaatacgt acttcttgtc
     3841 cttccaataa cgtcaaaggc aactgtcttt tctctaattc ggcttgtaac gaagcgacta
     3901 gagaaatgac ttgtgatttt tcattgctgt attttccatt attgtggtgt ggtgtgcata
     3961 aaatatgcgt aatgccttgc tgaatcgctt tttcagccat tgcaatactt gcttctaagt
     4021 tttctgcccc gtcatcgatt cctggtagga tatggcaatg tagatcgatc atttctcttg
     4081 tctcctctct tcttattcct taatttccgt agtaataacc agcatctttg ctgtgttctg
     4141 ctccgttgta tacgacacct aagactcgtg cttgtaccat atctaacagt tcttttgctt
     4201 tagtcaatga ttcttttcgt gacgtatttt cccgtacgac caataaggtg ccatccgttt
     4261 ttgaagacat gatctgtgca tctgttacgg caacgactgg cggcatgtcg aagatcacta
     4321 catcaaatag ttgtcttgct tctgctaaaa tctgatccat tcttggcgaa cttagtagtt
     4381 ctgatggatt tggtggttta ggtccacttg gcagaatcga caaattgtcg actggtgttc
     4441 gttggatctc atctgctaca cttcctgatg aactaagaac agtacttaat ccgcttgcgt
     4501 tgttcaactt gaacgtttta tagatgacgg gcttacgtaa gtctgcatcg actagcagca
     4561 cccgttgacc agatttggca aaaacaacgg caatgtttgc tgcagtggtc gatttccctt
     4621 ctccaggtcc tgatgaagtc acaacaatcg ttttgatttg ctgaccagct gcggaggcaa
     4681 actggatatt cgtccgaatc gtacgatatt gttcagcgat tggtgaagag ggattggtga
     4741 gtgtgaccaa actgactgca tgagtttggg ctgctttttg tttctgtgtt cgtgccattt
     4801 tctttcctcc tagactcttg agcggcttct gcgtgatggc gctgttttat cgtttgtagc
     4861 ttttggttgt gtctgagctg gtcctttttt acggatacca gcattcaatt ctttcgctga
     4921 catttctgga actgttccta agattgggaa tcctaatgta tctgtcacat atttttcatc
     4981 tttcactgtc cgatcgagta attccaataa gaatgccaga cctacaccta ccatcattcc
     5041 taacattaag ccagtcatta aattcaattt attatttggc gaaacaggtg tcgtattggc
     5101 aactgcacct gaaatgatcg agattttatc aacattcatc acatcttttg cgttctcttg
     5161 gaaaacttgt gaagttgtgt tagcgatctg ttgagcagcc actgcattcg tacttattgc
     5221 ctgaatcgag aacatttgtg aattttctgc ctgattaact gagatggcat ctttgatttg
     5281 ttcagcagtc attttcacat tgtcttctgt ttctaaacga tctttgactt cattgagcac
     5341 taaatcactg acgatcatat ctttatacgt attgatcatt tgcaggttcg tattgacatc
     5401 attgacattt gcagtgtctg attgtggcag tgtcacgatc aattgtgctt gtgaactgta
     5461 ttttggtgtc aagatgaaaa atgttacgat tcctgagatg gccaagccag caaacattga
     5521 gatgatgatt attaccaagt gtttgcgtaa tgtttcaaaa atttctttca agctgattgt
     5581 ctcttccatt ttttctccac tttcctattt attctctaga ttcatttgat cttttaattc
     5641 ttttttcaca cgatcaagtt cttgatcact gactttttga taggagatat tatcttgcat
     5701 gaagccttct ccttgcagct gatcttgctt aatcgtacca aatgcctccc gataatctaa
     5761 agcaattttt ttcatttcat caaagttcat gtctgttttc atattcgact ctagagcatt
     5821 caaaacggat tgatagttgc tgacaccgtt tagactcaat acttttttcg ctacgccttc
     5881 tacgatcttt ctttggcgcc cttgtctacc gtaatcacca tttgggtctt cataacgcat
     5941 acgagaataa gataatgcct gatccccgtt caatgtaatt ttaccgattg gaaagtcata
     6001 gccatcttgt gagaaagtga ggtcattatt gacttcgata ccaccaactg catcaacgag
     6061 ttcctttagc cctttcatat tgatagagac atagtgatcg attgggatgt ctacatattt
     6121 ttgtacggta tccatcgcca tggcagctcc gccgaatgcg taagcgtggt tgatcttgtc
     6181 ttttgtgtca cgacctacga tgtcgacata cgtatcacga ggaatgctga cgatcgttgt
     6241 ctgtttgtct tcaggactga ctgtggcaac catcatcgta tctgatcttc cttgatcgac
     6301 ccgacctaaa tctccagtat caatccccat caatagaaca gagaaagatt cttgtttgtc
     6361 cagatcaact ggtttcgttc gttctccttg ggttcgttcc actgattcgt aagttttctc
     6421 aatagaatgt gagagatcca gatataattt tgctcctact cctacaattg ctaatactaa
     6481 tacgataaga actgctaaaa taatcagaaa tatttttttc cctcttgatt ttcttcttcg
     6541 tcttgccata gtttcctccg tttgctaacc ttgcttgaat caacgtttta actgtcaata
     6601 gcttatattg ctatagccca tttgctcagc aacagtttgt gcttttgttg cgtatgctgc
     6661 gcttattttt tcattccgga tctgctctat ttgactgagc accgtcaaat attgatcgta
     6721 agtagcggtg gcagtcactt gaccatcctt taacatcgta tcgaataact gatctagttg
     6781 cgtgacttgc gcgatctgaa tatcagcgaa tcctagatat tgcataacaa ccgttttcca
     6841 aggactatct ttaaaaaagc tcatattttc acgtacacga gcgacatctg cacttgtcac
     6901 cttttctttg atgatgacat cgtctacagc ttgctgccag tttgatacat ttttagtaaa
     6961 taaatcgttg acagctgtct ggatcttcag ttttgattct gcactatcta actgtgtcac
     7021 gattgctttc ttctcatctt ggattgcaac agcttcttta ggcaattctg attctttgat
     7081 gtcgtaatca tctgctgaaa gtttgacaga ggatagctta tttgacaaac ttgtgattgt
     7141 ttcttctgtt atttcagggt ttagaaaaat tttttctttt gtactataca gcttgtctac
     7201 ttcttttgat aaagaagtca aggtgtcttt ttgattggtg atggcagatt ctgcataatt
     7261 gatccgttgc tgatgttgcc gctgattatt gatatggtat ccaaacaaac agatcgcaat
     7321 agcacctgca cctaccgcca cagcagtcaa tgacttgacg aatggctttt ccatagattc
     7381 actcctaagt attgatgata gataaattaa gatataattc acttacataa aaatgacagc
     7441 tttttattta aaaaaacata attaaactaa tatattcaca gaaatatttg taaacaagtt
     7501 ttccctatat aatcatacca caaactgttt tcttgactag ggcaatttac gatttaaaag
     7561 taaataaaac attaataaga taaaaattaa tcgatttcat ttttatttcc ataattttca
     7621 taggtgtttt taaaagttac acaaaaaata gaataacaaa aaaattaaca ttgaattaat
     7681 aata
//
LOCUS       DBNICI010000127         7656 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.128, whole genome shotgun sequence.
ACCESSION   DBNICI010000127 DBNICI010000000
VERSION     DBNICI010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.128"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(118..786)
                     /locus_tag="AC1ZCC_002209"
                     /pseudo
     CDS             complement(118..786)
                     /locus_tag="AC1ZCC_002209"
                     /inference="COORDINATES: protein motif:HMM:NF012488.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(811..996)
                     /locus_tag="AC1ZCC_002210"
     CDS             complement(811..996)
                     /locus_tag="AC1ZCC_002210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HIQ1630440.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            1223..2516
                     /locus_tag="AC1ZCC_002211"
                     /pseudo
     CDS             1223..2516
                     /locus_tag="AC1ZCC_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744739.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2603..3634)
                     /locus_tag="AC1ZCC_002212"
     CDS             complement(2603..3634)
                     /locus_tag="AC1ZCC_002212"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1630441.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(3641..4471)
                     /locus_tag="AC1ZCC_002213"
     CDS             complement(3641..4471)
                     /locus_tag="AC1ZCC_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1630442.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(4468..5283)
                     /locus_tag="AC1ZCC_002214"
     CDS             complement(4468..5283)
                     /locus_tag="AC1ZCC_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1630443.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5280..6104)
                     /locus_tag="AC1ZCC_002215"
     CDS             complement(5280..6104)
                     /locus_tag="AC1ZCC_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIQ1630444.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSENMQDIQELMRG"
     gene            complement(6094..7194)
                     /locus_tag="AC1ZCC_002216"
     CDS             complement(6094..7194)
                     /locus_tag="AC1ZCC_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIQ1630445.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7372..>7656
                     /locus_tag="AC1ZCC_002217"
     CDS             7372..>7656
                     /locus_tag="AC1ZCC_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIQ1630446.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPY"
BASE COUNT         2276 a         1858 c         1410 g         2112 t
ORIGIN      
        1 ctcaaacggc attatcatcg actgtttaaa cgaccaatta gtcaaacaga aatcgtcgat
       61 gaaccgttgt gcacaatggt gtcctgaacg tacagcgaca ccttccatat ccagagcagt
      121 cgccacatca cggggatgga tgccatccaa attaaaagca accacgccag tatgatgttc
      181 aggatcttgc ggtccgtaaa tggtgatacc gccgatcgct tgtaatttag gtaaaacgta
      241 ggcaaccagt tcttgctcat aacgatgaat attctccata ccaatctcat tcaagtaatc
      301 gattgctcct gcaatgttcg gagtaccagc ttcaaatttc catggcagct ctttccatga
      361 actatcgtaa agattgacaa aatctatcat ttcaccgccg aattcgactg gctccatttg
      421 ttctaacaat tgacgtttgc catataatac accgattcct gtcggcccgc acattttatg
      481 cccgctaaag gcgaaaaaat ctgcatccaa cgcttgtaca tcaactgtca tatgcggtgc
      541 tgcttgggca ccgactggac tatcctcaac tacaacagcc tgttcaaccc tcagccgttt
      601 caagcacaac aaataacctt ctggatctgg tttgccgacc tgaagatcat cgatcgtcac
      661 aaaggaatca attttttcct taatcatcgg atgtgcttca aaaaaatagt ctagctcccg
      721 cagttcagaa ttgagactac tccgattttc cagtgctgct gatccgctgc tcaaaatgtt
      781 cagcatccac tggtctccca accgcttgac ttatcccttc ataaaaggac agttctgctg
      841 ccgtcccaac accttgttga tagtctgcaa aaggaatttt atagccgaac tgctccagta
      901 aataggcttc agtaaatagt gatgaatact ctcgtaaaac atccattcac tgtcaatcat
      961 cgtcccatca aaatcaaaaa taagtgcttt ggtcatcttc tttccccctt acccaaaaca
     1021 agcaaaagct gtgtcctgct ctctctacag tatcagcata aaaactgccg tctgaaaagt
     1081 caaaggtgaa agaaatgtgg cgctataatt tctaggactg atagtaaatc attctatcag
     1141 tccttttgag cataaaaaaa caaaacatac agaagctccg ttagaattga atcaccacaa
     1201 cacaatcaaa ggagcgttct gtatgcctga tcttcatctt atcaaactat tactaggaat
     1261 caaagatgga aacattcaaa tcactcatgt cgagaagaaa aaaatacatt caatcaacag
     1321 tatcgttgtt tctgccacgc tcattaaaac gattcagccc tgtcctcagt gtaaacaggt
     1381 gaatcaacat gggctgatcg ttaagaacgg aaagaaaaaa tcgctggtcc ttctcaataa
     1441 gtgtgcgaat caacggactt atcttgcttt ggcaaagcaa cggtacctct gtcgtggctg
     1501 tcacacctat tttacggcga atacctacat tgttgaaaaa aactgtttta tcgccaaaca
     1561 agtacattat aaaatattgg aggaattgac tgaaaaacaa gcaatgacga cgatttctaa
     1621 acattgcggc gtttcttggt caaccgtttc gcgaacactc gcttctctgc tccccatgac
     1681 aaacgtgaaa ataaattggt tgcctcgctg ttcattggta gatgaatttc gttcattgaa
     1741 aaatcaagtc ggtccctatt ctttttcttg tatggatggc gatactggga aattactgga
     1801 tattcttcct tcacgtaaga agaaagatct catttcttat tttatgcaat ttgaacgacg
     1861 tgcacgttta aacgtaaaaa tactcgtaac agatatgaac gcctcttatg ctttgctgat
     1921 aaaagagtgc tttcctcatg ccaaactcgt tgtggatcgc tttcacatcg tcaagcacct
     1981 cattcgcagt tttaaggata tttgactatg agtcatgaaa agcttcgacc gaaacgatcc
     2041 aatacaagcc aaacactatc gacaagtgaa ggctttatct cgacttctga tcaaacgaca
     2101 ggacacgctt gtttatgata aatggacaaa atggcgtaat tttggttggg cctatttaac
     2161 cgaaagtgaa gtcgtagaac gtctactttc tacttctgat gaactacgaa tagcttatgc
     2221 ttactatcaa gaaattcttc aagcttttta tgacaaagaa gcggacatct tctttcagct
     2281 tgtaaaaaca atgcccaagt cggttccaag agaactgcat catataaaaa aagcgtttat
     2341 aaactatgaa tccggcattc gtttagcctt agagttgcct tactcaaatg gcaaaataga
     2401 aaatctgcat acccacatca aagcattgaa acgaatagct tacgggttag gatgagaacc
     2461 agaattttct taatcaatca tctgatcact tattaaacat aaaaaaacat ctaaagcaga
     2521 aaatcaattc taccttagat gttcttatat cactctatca gtcctatttg acaaagagcc
     2581 agaaatgttt gccgactata cactagcttt gttgtgcaaa atgctgagta tcaatttcca
     2641 cagggattgc tagaatatct ttcagctgaa tgtataaggc atcgctaatt ttctcttccc
     2701 gatcgttcaa ccgatccact aaaatttctt tgccctccag cgtttgaaaa acataccgtt
     2761 gaatgttgcc taaaataaat gattccttcc atagaaccgg aatcttaacc ccctgaggaa
     2821 ccggttcaaa ttgaatcagc tccggtctta aataataaat gcagcgattg cccttagcgg
     2881 aaaactcgtg ggtatactga ttcagctcat accattcgaa acgattatag ttgccgataa
     2941 attccgcaac aaatcggctc tgaggattct gatacaattc tttcgaggtg gaaacctgag
     3001 ccacccgccc agcttccatc acaaaaatcc gatcactgat tcgcatcgcc tcttcttggt
     3061 catgagtcac aaaaatcatc gtcatcccta gcgattgctg gatagcgcgc aaatcccgct
     3121 gcagctgttt tctaattcgg gcatccaaag cacttaatgg ctcatccagc agcaggactt
     3181 tcggttcagt gaccaatgag cgagccagtg ccacccgttg tcgttgaccg ccagaaagaa
     3241 actggacatt tttatctgcc tgctccgtca tatccaccaa tgatagcatc tcaaaaacct
     3301 tttcctgaat cacctcagga ctcagcttct ttaccttcaa accaaaggct acattttcaa
     3361 aaacagtcat agttggaaac aatgcatact gttgaaaaac aaagccgata ttgcgatcct
     3421 tcgtaggctt atcggcaata ttctcattgt ccagcacaat ctcaccgctc tttggttgtt
     3481 ccaatcccgt gatacagcgc agcaacgtag actttccgca cccgctggga cctagtaaag
     3541 taaccagctc acccttttca atctgaattt catcaatagc taaaatctgt ttttcatcaa
     3601 aaaacagatt gagatcatgt atttctaaaa acattttccg tcacaactcc tttaagggct
     3661 tctttttcga ctgcttctct tccctcgtct gcttaaggga cagctgttgc ttttgtcgat
     3721 aagtcagcat aaaggtagtt aaagcaattc ccaccataaa cagcaaatac aaaaccatca
     3781 cagcgctagc caaatggccg ttttcgttca tccgccgcag catatagatg cgtaccgtct
     3841 gaaatcgacc gccaatcaac aggttcgtca gcatgtactc cccaaaaacc gaagaaaaaa
     3901 tcatcagaga aactagggtc acaccgatac ggatgtttgg aaacagcacc tggataataa
     3961 tcgtagacat cggggcgccc aaggtttctg ctgcatccac taattccttc agattaatta
     4021 agcgcatctg gttgttaaga ctaagataga caatcggcaa cgccgtaata aacaatgcac
     4081 ctaccaacac tacaaacatt gggataccag tttcagcata cgttcgaagc aaagccgtta
     4141 ccaaaatcac tcctggtaaa gcatacggca gcaggagcag cttttccagc caacgattca
     4201 aacgagggaa atacaaatga atccaaataa tggtgggaat catcaacaac agaatcgtca
     4261 ccaagaccac acctgctaat aaaagtgagc gacccaccgc agcaataaat tccctgtccg
     4321 tcagcagctg ttggaaccat tgcaaggtaa aatcactagg taaaagcgat tttgaccatt
     4381 tagtggagct ggcatacaaa atcgttccaa taatcggcag caagtaatac aggacaaagc
     4441 agaacaaaat cggttttatt aactttttca cgtctgcacc accttgcctt tcttttgaat
     4501 aatcaacatt gccgttacca gcaggacacc taaagcgact gccaacgcac tgcctaaatt
     4561 aggcttggcg aacacatctc ccgacactaa agccgcaatt cgaatcgtta aaagatttac
     4621 attactgctg gtcagggcat acgctgtttc ataggtcccc atgccattag caaataagat
     4681 gacaaaggtg ctggcaatcg atggtcgcaa aaaaggcaaa gccaccttga accaagcata
     4741 cgctgatgaa gcgcccagca attgtgcgga ctccagccat tcctttttca cttccttcat
     4801 gctgggatac aaaaacaaga cccctaacgg aatttggaaa taggtatacg tgatggtcaa
     4861 tccccaccaa gaatacaaat tgaagtccgc tagaaatggc aaaatcattt tcagcacacc
     4921 tacattcccc aacaaaataa taaaagagaa tgccagcgga atcccagcaa aattagctgc
     4981 caaattggaa acaaaggtga ttttctcctg caatttagtc gatagattca ttaagcataa
     5041 acataaaata aacgtcccga ttaacccaat cacactggag ctgacagcaa tcagtacgct
     5101 gttaacaaag gcctgtgtgt aatatttgtt gctgaaaata tcactgtaat tggccaatgt
     5161 ccactggtta gttaaatcac tgtgcaagct gcacagaacc ataaaaagca gcggcagcag
     5221 taaaaatgca cagaagataa aggaggcagg aaccagaacc aagcgttttt tcatctcttt
     5281 catcccctca tcaattcttg aatgtcctgc atattctctg acggtgcaat tcctaataac
     5341 cagcaaaaca atggagccgc cagcagctgt ggtacagttt gttccttcag cttttggcgt
     5401 ttatttgaaa tcaaataaaa aggaacttca cgatgtgcag ccaagctgcc gccatgcagt
     5461 ccgtgcgcat ccatcccgtg atccgcagtc accacgactt gataaccttt ttgcagccat
     5521 tcaggaaggt aaatagccaa taatctgtcc gcctgattaa ccgctgctgc atattcatga
     5581 gaatcggcgg taaatttgtg accgacatca tcaatattca tcgaatgtac gaccaaaaag
     5641 tctggctgat actgattcac taaataatgg gcatccgcaa ataaatgcga atcagggtaa
     5701 gtgtcctcac tataaaagat gccatgctga atcagtcgct cctcctgatg ctggatgcga
     5761 tcaaccaaac gattataggg ggcttgattg tacaactcgc tgtaccagtg gtaagcggcg
     5821 gctcctgttt ttccgccagc ttccttaact aattcaaaca gcgaccactc tttggatcgc
     5881 tgagcatggt aattactata gatgccattt tcataggctg gaacgccggt catcagtact
     5941 tcgtataacg gacgtgaatt gctgggcagc tcacttttta ccttcaataa gcttgcttgc
     6001 tggtgctcaa ctaaatgatt catgaaccca agctgggcga cggctgtgtc gtagcgacag
     6061 ccgtctagta taactacaat cagtttgtca agtctacttt gcataggtca gtacctccgc
     6121 ctgccaaagc tctggaagct ctacagaggt cttgttccaa gcttcattgt cttcaactgg
     6181 caccgccgct tcataagcat cctccggcag taatttttca gctacttctt taggcagctc
     6241 taccttgcga ataggtcgcg catagccctt tgccaaattg atttgccctt catcagataa
     6301 aatatattcc cgtgctagct ttgccgcatt gggatgaggg gcatttttat tgattaaggt
     6361 tgtatacccg ctaatgatgg agccgtctgt tggcacagtt acctcaaaac gctcggagtc
     6421 aatttggtca cgataattta gtccattgaa gtcccaaaca actgccacat cgatttcacc
     6481 tttttcaaga tttgctacgc tggcatcaac ggtggacaaa cggccttttt ctgccatttt
     6541 tttgtagtaa ttcagacctg gctgaatatc cttttcgtca cctccgttgg caatcgccgc
     6601 agccaataca gcaaattgtg cttggttggc aatcgtcaca tcccccacgc taacttgata
     6661 gtcaccctct tgcagctcct tccagctagt cggtgcttgc ttcacattct ctttatcggt
     6721 gataaaggaa atggttcccg tatagcttaa caaccaatcc ccatcatcat cctttgccca
     6781 atccggaatt tcctcccaat agttggtctt ataaggtaag gttaactctt tgctttcagc
     6841 caacggaccg aagctgatac cgacatcacc aatatcggcg gttccatttt ttccttctga
     6901 ttcaaactta gccaattctt ctgcactgga catatccgta tccacgtgac ttaagccgta
     6961 ttctttttca aggtcgttcc aagtgcctac ccaatttgcc caagtatccg gcattcccaa
     7021 gctggcaatc tctccttctt ccttggcttt ttctataatc gtatctaaag agtcgtctgc
     7081 cgcagccgat tgcttcgttg cttcacctga gccgcagcct gtcagcagga tacttactgt
     7141 tgctgcacaa aataaaccga tagtcatgtt acgaattttt tcattcattt tcattcattc
     7201 ttctcccctt tcgataattc cattctaaca aagctttgta atgagagaat cagtttcttg
     7261 aaaaggtttc atgaacaatg taaaggttgt atatggtttg taaatttcga cgaattcccg
     7321 ttaaccttct aaagcttttg cggtatgata gtagaaaaaa ggaggctccc tatgcagtat
     7381 tatgatcagc atctccacac gtatttttcc tttgattcag aggaaaaatt tgaaaactat
     7441 ttagcctatc agccggagtt tttcgtttct acggatcact ttgatttaaa caatccatct
     7501 accggaaaag atgacattcc ggattatcca gcctatataa acaaactgga ctctttaaaa
     7561 gaaacctatt ccaccaaatt tctgcgaggt atcgagattg gcgttgttcc aggacaagaa
     7621 gagcggatcg tggattacct cgctcagcat ccttat
//
LOCUS       DBNICI010000128         7638 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.129, whole genome shotgun sequence.
ACCESSION   DBNICI010000128 DBNICI010000000
VERSION     DBNICI010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.129"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..2670)
                     /locus_tag="AC1ZCC_002218"
     CDS             complement(<1..2670)
                     /locus_tag="AC1ZCC_002218"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIQ1630447.1"
                     /translation="MNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFICY
                     LLAVSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLFVHKSYFLVAAF
                     FYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLLLLISNRFW
                     YALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVSPVLLIVGGLIL
                     LLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSPSFLLFNLF
                     KVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYNTKADEINQ
                     TRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLMLSVGYGGG
                     TANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHPFTAALYRR
                     KQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNTETSQFIQL
                     STMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQSLHYTDTAVKKFIQEIDKID
                     KPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNKEVVAPNNF
                     GAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTEDSQIIQEK
                     NLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
     gene            complement(2657..3019)
                     /locus_tag="AC1ZCC_002219"
     CDS             complement(2657..3019)
                     /locus_tag="AC1ZCC_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630448.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFLRTSWQLEYS"
     gene            3238..4458
                     /gene="sstT"
                     /locus_tag="AC1ZCC_002220"
     CDS             3238..4458
                     /gene="sstT"
                     /locus_tag="AC1ZCC_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HIQ1630449.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            4605..5240
                     /locus_tag="AC1ZCC_002221"
     CDS             4605..5240
                     /locus_tag="AC1ZCC_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HIQ1630450.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            5431..6171
                     /gene="truA"
                     /locus_tag="AC1ZCC_002222"
     CDS             5431..6171
                     /gene="truA"
                     /locus_tag="AC1ZCC_002222"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIQ1630451.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(6316..>7638)
                     /locus_tag="AC1ZCC_002223"
     CDS             complement(6316..>7638)
                     /locus_tag="AC1ZCC_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630452.1"
                     /translation="TMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGE
                     AVALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSF
                     YFWDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLT
                     HILNNNYNNYTIGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFN
                     HTPVKSLQFVWRLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVS
                     NSIHANKDSLTLIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTT
                     HFMSNKLTIELDNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGI
                     NKVVITYQYTKLAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         2497 a         1197 c         1444 g         2500 t
ORIGIN      
        1 tttttgtgtc gcccatgttg cactgtactg gttacctgca acaagatcat attgtatcag
       61 acgataatct tctagaattt ctttttgttt ctcagttaaa tttttttctt ggataatttg
      121 actatcctct gtaacaaaga cctgttttcc attaaaacga ttagacaagc tttctgtagg
      181 atcaattgtt aatgcaggta gatgattaga tacttctgtc aacaacgcat agtaaggagt
      241 tactttgata ttcgcatgct caagagccat agcaccaaag ttattaggag caacaacttc
      301 tttatttaac gtagctgctt gttcacgact atacttgttg ttataaatga aataatctgt
      361 ctggtgctgt tctaatccat attttttcga atcattccca ctatagattg cgggtaaatg
      421 gtcaccataa aaaacaaagg taatcggttt atcgattttg tcgatttctt ggatgaattt
      481 ttttactgct gtatcggtat agtgaaggct ttgcatataa gtttgtaatt cttttcttct
      541 attaggaatg acagcagttc cttcagcagt gtagttcaat tcactatagt aatcatcata
      601 aggcatatgg ttttgcatag tggataattg gatgaattgt gacgtttctg tattgctttg
      661 taaagctttc aaggtttctt tgtatgctga atcatccgat atgcgtgggc tttgttctat
      721 tttatccgta taagttaatt tatccggact gtctaaataa tagaacctgt caaaaccaaa
      781 tttatcaaac acttgttttc tgcgataaag agcagctgta aaaggatgga tggctatttt
      841 ttcatcgaat agatcggtaa tatttggact tattttctga cgctccacta gctgggtata
      901 aggtgtaact aacgaagcag acaggttact gatactcaag cctgtcagtc cttcccattc
      961 catgttagct gttccgccac cataaccgac ggataacatc aatcctcctg ttccttctgt
     1021 tataatgctt ttgatgtaag gaatagggtc tgtaggtact gtcagattag ggacacgtgc
     1081 aggatcactg aagctttcac ttaaattgag aacgatcgtc tgattgttta accagtcact
     1141 tcttgtttgg ttgatttcat ctgcctttgt gttgtacttt ttcattattc cattaatttt
     1201 ttctttcgaa tagccagcag gttcttccat gattttaatg tcgatattgt ttaggaattg
     1261 aatgactggt ccgttatctt ttacggcgcc ttgctggtta aagaaagtct tgtttacttt
     1321 aaataaatta aacagcaaga aagaaggcga atttttgtga ttaatgaaaa agacacccga
     1381 aaaacatcct aataatacag cgatactgat aaatcgacgt ttccaatgaa tcttcaagga
     1441 atactgtttt tgtagtctac gttgtacaat aatactagta atcaataata ggagaaggat
     1501 caatccgccc acgattaata agactgggct aaccatcgat aatagttctg acaatccggt
     1561 cagtattttt aaatctgaag gaaagacagg ctcttcccgc atttccattt tgattactgt
     1621 cgaaatagtg agcagtaagt taatgatcaa ggtagcagtc aaggcgtacc aaaaacgatt
     1681 ggaaatcaac aatagtaaga agaaaaatcc cataataatg atgattgtca ggatcatggg
     1741 tgcttgtcgc tgagtaaata tttttaaggc aacctggata gtttctttcc aaccttcttt
     1801 tggttctaaa acgaaaatct gcacgaaggt aaagcagtaa aaaaatgctg caactaaaaa
     1861 ataactttta tgaacaaaaa gccaatgaat tctttttttc caacgctgag ttaattgttc
     1921 aatcgaatca aaagcgaagt attcgatgaa acgatgaatg aaacggattt tttgtatata
     1981 aaataaaaag ataactagga gggacactgc taataaatag caaataaaaa actgcccgat
     2041 atttatcagc catgccttcc ctgagtttgg aaattcacgt ttcataaatg tagatacgcg
     2101 ataagaaact aaagggaaat tacatataac ttgagtgaca atcaaaaata cagcaagaaa
     2161 atttcctctt ccttttatac ctaatttatc agatagcatt tctaaaaata aaaacgaaga
     2221 gaaagtataa tacattgcga agagagaata tggtttagaa aacctcagcg ctgtatctgt
     2281 atttcctctg atgatgagtg aaatttgtgt catcaaaata atcaaaccga ataaaacaat
     2341 caagctgata aatacttgag gaagcttata gcggaccttt tctttccagt tccattcttt
     2401 caaaccataa cccattaaaa ataaataaaa aatccataaa atactctgtc cattctgtaa
     2461 tgcaaacggg tctttattaa atagagtaga gcagccaact aatccaattg ttaaaaataa
     2521 tagtagatat ttaattgttg ttttcgataa tttatttaaa aatccaactg tataaggaat
     2581 actacaaaaa agcaaagtac aagaaacagc ataagtaaag taattctgac tgataggtgt
     2641 taggataagc caataatcac gaatattcaa gctgccagct tgtacgtaaa aaaaagatat
     2701 tgcaaactag tgtggctatt aggaatataa aggcaaaacg acttaaataa tgtaaaactt
     2761 ttaaacgggc gattttttga tattggtaac ccatgtaaag tgggatcaaa tttgcaccaa
     2821 taacaacgga agtttgtaaa aatgtataga tgtagaatcg accagggttt gttgcatatg
     2881 taggaatagt gactgctgaa atcaatatac gctgtaaaac catcattata caagcgacaa
     2941 agattgtcag gaagccataa ctctccttct ttattttttt caagaataaa cacctcacaa
     3001 aaaaattgga caaaaacata agaaaacgtt tcctgataag ttgcccatta ataacatcct
     3061 aacttagaat atctcaaaaa acaacgtgta gacagaaaaa gtacaaaatt agcaagaaat
     3121 ggttgtttgt attgttagaa tttttagaaa attatacctt tagtttcttt gcataaaatg
     3181 atacaatgat tgttaaaatt gtgaaagggt ggtcatttgt tagagaagtt tttggatatg
     3241 tcgttagttc aacgtattgt tgttggtatt ttagtcggaa tattgtttgg tatctttttc
     3301 cccacatgga catttatttc aatattggga acgttgtttg tcagttgctt gaaggcaatc
     3361 gcacctattc tggttttttt cttgacgatg gcgtcaattg ccaagcataa aattgggaat
     3421 aagacattcg tgaagcctat tttgatctta tatttagtgg gaacattgct ttctgcactt
     3481 gtagcagtta ttgtaagttc aatttttact gttcctatta ctttacaaga aacagtatct
     3541 gaaaaagcac cgcaaagttt ggaaagtgtt ttatctacta tgttgacgaa tgtcacacaa
     3601 aatcctgttc aatctcttat tgatgcgaat taccttggcg tcttattctg ggctgtattg
     3661 ttgggactag cttttcgagc acgttcagaa tcgacaaaag agttggtgga tcagatatct
     3721 atagcgttgt cacaagtcgt acaagtgatt attgcatttg ctccgatagg gattttagga
     3781 ttggtttatc aatcaattgc tacgactgga atagctggat tggcagagta tctgcagctt
     3841 ttacttgttt tgatcggtac gatgctgttt gtcgcattag ttgtgtaccc attcctcacg
     3901 tttttgttta ttaaagaaaa tccctatcca ttgattttct tttgtttgaa agaaagcgca
     3961 cttcccgctt tctttacccg aagttctgcc gcaaatatcc cgataaacat gatgttggct
     4021 gaaagattga aattgacaaa agagtcttat tccatatcga tcccgttagg tgctacgatc
     4081 aatatgggcg gtgccgcgat taccatttcc ttgatgacgt tgactgctgt acattcttta
     4141 gggattgaag caccttttgt tttgaaagtc cttttatgta ttttgtctgc tttagctgct
     4201 tgtggagctt caggtgttgc gggcggttca ctgttattga ttccattagc atgtagtttg
     4261 tttggtatct ctaatgatgt cgcgatgcaa atcgtcggta ttggatttat tatcggtgtt
     4321 atccaggatt caatggaaac tgccttaaat tcgtcaagtg atctgttgtt tactgcaata
     4381 ggagagctag gcgcgcgtaa aagaagtggg gaaaaaatca aattgaaaga ttgtttagat
     4441 ggattaagtg aacgttgaac gttagatatt tagtattttt aaaatgattc gagtttttaa
     4501 tgagttatat agatgttcgt gtaaatagag gtttctgtta aatcaagata aagtcgctta
     4561 taataacaat taaaagcata ggtaattgtt attggaggcg tatcatggat tttttacgta
     4621 aatttggttt tttcttttta ttattaggcg tgctgagtga ttttttgact ccgtacgtat
     4681 taggattgta ttatcctgaa ttagatcaaa tgaagagcgt gatcagtgta tttggagagg
     4741 tttccagtcc tgtaagaaaa gcgtttctta tttggtctgt ggtttcaggg atctttttca
     4801 tcctgtcatt gcctgcattg tatgctgcat ttgtaagtac atctcgtgtg ttagctaccc
     4861 tagttacagc agccatcgcg ttctatggta taggtgattg tatctttaca ggtttgttca
     4921 gtatcaatac ccatgaatct tcgtggaatg tctcgacatg ggtccataat atcggatcag
     4981 gtttaggtta cagtggtttt ttattgtttc cagttcttct tttcttgatc tatcgaaaaa
     5041 atgggcaaat ggacgtaagc cacttatacc ttggactgtc tgtgatcagt ttgctttttg
     5101 cggcgattta tggactggca agaattcctt ctataaattc ttggccatta ctgaataaaa
     5161 taggtttttg ccaaagaatc agttttttct ttaattatgt gcccattgcc tggttgagta
     5221 tcttgcatct caaacagtaa aacttgagcc gtcaaattga acagtcagcg gatacttcat
     5281 ccggtggctg ttttttgttt ttctgaaata tatatttcca cagttttctt gtatagcttc
     5341 tgttttagag aagaaaggag ctatattttg tgctacaatc ttaaaaaggt agagagaaga
     5401 ccaaatggaa gacagaggag ccactaaaat atgagaaaca tcaagttgac aatcgaatat
     5461 gacggaaaaa gatattcagg atggcaaaga cttggagatg acgataaaac cattcaagga
     5521 aaaatcgaaa aagtattgca tcagatgaca aatgaagaga tagaaattat tggatcagga
     5581 agaacagatg ctggcaccca tgcgcgagga caagtagcaa attttaaaac aaacacagaa
     5641 attggactgg cagaaatgat cgactttatg aatcgttacc ttccgcggga tattgtcatc
     5701 aaaagaatcg aagaaatgcc tgagcgcttc cattcaagat ataatgcggt gggcaaaaaa
     5761 tacagctatt atgtttggaa caacgtgatt ccttcagcat ttgagcgaaa ccacagcttt
     5821 tatttccctc aagagttgga tatggacaag ctgaatgcag cttgcgaaaa attagtgggt
     5881 acacatgatt tcattggttt ttctgcattg aaaaaatcaa aaaaatccac agtgcgtacg
     5941 attgatgaat tatctatcga acgaaaggga gaaatgcttc attttacttt tgtcggagac
     6001 ggattcttat ataaaatggt gcggattatc atggggacat tactagaaat cggcacaggt
     6061 gccatgccga tagaaacaat cgatgaaata tttgaaagca aagtgcggca acatgcagga
     6121 gaaaccgtcc ctgcacaagg tctgtttttg gatgaagttt attatcgata aaaatacttt
     6181 gataatggat gaaacgcatg tttcacagta taaatatcga tgttaaataa tgtatgaagc
     6241 aaattaaaga aatataaaaa aagacttttt agcctgttta tgtgtacagc taaaaagtct
     6301 ttcttaaata aaaatctagt gagaatgttg tacttctctt ctcggttgtt tttgtttaga
     6361 aaaagtaaat cgataaagta aaaataaaat caaagtaacg atagataaat atcttgaagc
     6421 aattgctagt tttgtgtact gataagtaat aacgacttta tttatacctg gaggaatagt
     6481 taattcagtt gtgccaaact tagataattt tgtttgtact agtttgccat taatagaagc
     6541 cacttgccct ttataacgat aaataggtgt cgtcaagaca gtatttttat tattcgaatt
     6601 atctaattca attgtaagtt tgttactcat aaaatgagtt gttggattta tttcctggtt
     6661 acctagtaaa tattgatcat tattaatcat atcaggatga tagattgact ctttgtttgc
     6721 ataatcagta tgattgtaat tttcagctat agcaacagcg tcttcaggtg ctataagtgt
     6781 taatgaatct ttgttagcat ggatagaatt gcttactcct gagagatgca atattagtgc
     6841 aagaaacgta aagaccatta ttttccatga attattttta gtagaaaaaa tataatagat
     6901 cactatactc atcgtatagg ctataaataa agaagaaaaa ccatttaacc gccagacaaa
     6961 ttgtagactc ttaactggtg tatggttaaa aagttgccaa ggaaataaat tggttgaaca
     7021 aaaaagaaca aatacaccaa gaaagaaaat aaataaatca tcagcagtta atttttttat
     7081 aaaaataaaa gcaccaatca atccgagaaa taagaataat ccaatagtat agttgttata
     7141 gttattattt aaaatgtgtg tcagcatatc actaggagcc ataccactta attcttttcc
     7201 cagtgggact ttcaattctt gtgctctaga ttgttgaata aaaggaatta agaatcccgc
     7261 acttaaaaaa aaggtgagga cggtagcttt taaaagactt aagagtctag cgattttttg
     7321 gtcccaaaaa taaaaagaaa gaaacaacgt tcctccaata aatacagaaa ccatagcgac
     7381 agaaagtaaa tgagtataca ctactagcgt catgccaata gacaaccagt accatttttg
     7441 gtaatctcta atataaattt cgtaacaccc cattagtatc aaaggcaaaa atgttaaagc
     7501 gacagcttct cccaatgaag cacgatggaa tatgtcaatg gcacgatagg cggaaaatgt
     7561 ataaataatc gaaaataaca gcgaaatata acgattgttt tttatttgtt tcatagaaaa
     7621 ataagaaacg accattgt
//
LOCUS       DBNICI010000129         7601 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.130, whole genome shotgun sequence.
ACCESSION   DBNICI010000129 DBNICI010000000
VERSION     DBNICI010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.130"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(4..627)
                     /locus_tag="AC1ZCC_002224"
     CDS             complement(4..627)
                     /locus_tag="AC1ZCC_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630453.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(786..2183)
                     /locus_tag="AC1ZCC_002225"
     CDS             complement(786..2183)
                     /locus_tag="AC1ZCC_002225"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HIQ1630454.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(2215..3186)
                     /locus_tag="AC1ZCC_002226"
     CDS             complement(2215..3186)
                     /locus_tag="AC1ZCC_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIQ1630455.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            3560..3985
                     /locus_tag="AC1ZCC_002227"
     CDS             3560..3985
                     /locus_tag="AC1ZCC_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630456.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(4473..5174)
                     /locus_tag="AC1ZCC_002228"
     CDS             complement(4473..5174)
                     /locus_tag="AC1ZCC_002228"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HIQ1630457.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(5190..5630)
                     /locus_tag="AC1ZCC_002229"
     CDS             complement(5190..5630)
                     /locus_tag="AC1ZCC_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HIQ1630458.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(5630..6205)
                     /locus_tag="AC1ZCC_002230"
     CDS             complement(5630..6205)
                     /locus_tag="AC1ZCC_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HIQ1630459.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            6446..6655
                     /locus_tag="AC1ZCC_002231"
     CDS             6446..6655
                     /locus_tag="AC1ZCC_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HIQ1630460.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            complement(7053..7529)
                     /locus_tag="AC1ZCC_002232"
                     /pseudo
     CDS             complement(7053..7529)
                     /locus_tag="AC1ZCC_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         2170 a         1579 c         1214 g         2638 t
ORIGIN      
        1 ttttcattgt ccataggatt gatattgtcc ttgtgatgac tcattcgtaa taattgaggc
       61 ttgattttct tcacttcccg ttatcgtttg tccactgcca gaatctgaag gacttgttcc
      121 tgtcgactcg tttttttgag aagagacagt tgttttcttg acttgttcat ttgtcttttt
      181 ccattccgct aaacgatcgg cgaccacttt tttgtctact ttttcagaat aagcggtgat
      241 cacttttcca tttttcaaac gggctacttt gtcgccatca tgagtcaaaa gcaaaacggt
      301 caaatgcttg tctttaaatt gatattgtct gagagaaatc gcactaagta cgtcatcgat
      361 attaataaat tgattttctt cttttccttt tggcaaataa atgtccaatt cgcctttttc
      421 gtaattggca gttattttat actccgccca ccaataagtt gagaattctt ttttgaaatt
      481 atctactgat tctttgcttg ctacgatttt ttctgtttct ttctgagcaa gatctgctgt
      541 catttttcct gaaaatgctg cggataaaat aagcagtaag aaaaaaccga ataaccaaaa
      601 gagaagaaac ggtctttttt tcctcatgct ttcctccact cctatttaat tttgactatt
      661 ctattatata caaaaaatga aatagaaaga caaaaaagtt gaaaaagatc gtaaataatt
      721 gaacttcttt ttcacaaaat ggtaagaatg gagaatttca cgcatttgct ctcaactatt
      781 ctttcttaag cctcaaatcc gtttttatct gatacgtctt tgaaccaatg acccgatttt
      841 ttgattgttc tttttccatc ttgtgctaag tctactgaaa taaagccata tcgattctta
      901 tatgcattgg tccacgacca gttatccata catgtccaca tatgatagcc taaacaattg
      961 gcaccttctt gaatggcttg atggacatat ttcagatgag attggacaaa ttcgatccga
     1021 taatcatcgt ggatcatgcc atcttctttg ataaatcgtg cttcgttttc tacacccata
     1081 ccgttttcag aaataaagca gtcgatattt ccataatgat ctcgtacatt gatcaaaata
     1141 tcatagattc cttgttcata gatctcccat ccgcgataag gattcatttt acgaccaggc
     1201 atttcataat tgtcgaaata atcctcaggc attggaccgt ttttagtatc gattggtgtt
     1261 tcttttgctt ttacccggcg tggctggtaa taattgatac ctaaaagatc tactgtattt
     1321 tctttgataa ccgtcaaatc ctctgcatgg atcacaggca tgagatcaag ttcttttact
     1381 atctcaatca attcacttgg aaactcgcct tttacagctg gatcaaggaa tgaacgattg
     1441 aagaacgcat ctgctatttg cgctgcttta acatcttcag ggtttgattc gtctcttgga
     1501 tagcttggtg tcagattcaa gatgatacca ataacaccac cttgctccat ttcatggtaa
     1561 gatttgatgg ctaacgctga agccaacgct tcatgatagc caacttggat cgcttttttc
     1621 atatctgact cttctggata atgccatcga tataaataac ctgcttcgac tggaacgatt
     1681 ggttcattat gagtaaacca tttttttact ttcgaaccaa acaattcaaa acaacttttg
     1741 gcatattttt cataggcttc aaccgtttct cggttcaacc atccgccttt ttcttgcaga
     1801 tacatcggca tatcgaaatg atacagattc acaaagggtt cgataccgtt ttcaatcaac
     1861 tcatcaaaat aacgatgata gaaacgaacg gcttcttcgt tgattgttcc agattcatca
     1921 ggtatcaaac ggctccattg gatagatgtt cgaaatgaat tatgcccagt ctgtttcatc
     1981 aactgtacat cctcttgata cttcgtataa acttgcgagg ttttttcagg tcctacttga
     2041 tcaaagaact tttgcggttc ttttttgtac caataatcaa aaatgttctc tccttttcca
     2101 tcgcctgaaa tccttccttc tgtttgtggg ccacttgctg cagaacccca ccaaaaattt
     2161 gctggaaatt gatatttttt catcaaagca cctctttttc ttttttagtt cgttttagtt
     2221 gattccatgt atgatatttt ccatcacaat cactttttca ttttcttcct cttctttttt
     2281 gattcgtttt aacagctgtt ctgctgctcg ttccccgatt ttatctgttg gtggcgtgat
     2341 cgtgattttc ggaaggatac tcagatcgtt tttatctaaa taatcatagt gactgaacgc
     2401 aatatccttc ccgattctga caccttcttg attgaaacat tcgatggcac caatcgacat
     2461 attgtaattg ctgacaaaaa tagcagttgc ccctttttct aataatgctt ttgccccgta
     2521 atagccgctt tttctagaat agtctcctgt aaagacctct aattggatcg atggatcatt
     2581 ttggaccgct tctttggctc ctgctaaacg ttctcttgct acgtagtctg tttgagtggc
     2641 agcaataaat ccgatttttt tatgtccttg ttcaccatga tgagcgatca cttttgctac
     2701 actttctcga tcattcacta atattgtatc aatacctgct ggtccttctg gacaattcaa
     2761 tgcgataact ggaatactca tttttgtgag catttcaaat ccagaccact cttcttcgga
     2821 taagaagaca atcagcccat cgatcacatg cttcatcagg tattcaattt tttgttcaat
     2881 tttttctggc tgatcggcaa acccgcttaa taataagcta tacccatttt cttcagctac
     2941 tctttcgatc cctgagacaa cttccgaccc aaaacggctg gaaatcccgt ttatcaaaag
     3001 tccaatagag aagcttcgat tattcttcaa ccctttagca atgatgtttt ctttatagtc
     3061 caacgctgaa atcgcttctg ctattttttt catgttcttt tccttcagtt gctgaccatt
     3121 caagtaacgg gaaactgttc ccactgaaac accagccatc ttagccacat cacggatcga
     3181 tgccatacat tctcactcct gccagttaca tttgatttct ctttcttgtt gttcgattat
     3241 gatgaccgca ttctttgcat agtgttgctt gtcatcaata tccatataac taaagtcttc
     3301 agtagagcaa cttcctctca ctattaatta tacttaaaca agtatttgaa ccggttcatt
     3361 tttaatatag cattttttta aaagattgca acactttttt aaaacgcttt ctgaaagtgg
     3421 cggatttttt gcctttttaa aattttattc taaaatttac tctatttgtg ggtgttttgt
     3481 gcgtttttct cttccatccg tttgaagttt tacattccaa tggtataatt tctttatcct
     3541 atcaaatggg ggaagtattt tgaagaaatt aatcgtacta tttattttgt ctgcgggact
     3601 tttgacattg actggctgtg atttgtttgg cggctctgac caatcctcgg acgaaacaac
     3661 cgattcttct gtcttaaaag cggaaaaagc agataccacc aacagtgtat atacgctaaa
     3721 agtacgtaaa gatatggatt tatccgcggg acagttgaca tttacagata aaaaaatgga
     3781 atggacacgc acgtatcaga cacaaggaac tgattcttct gaatcatctg aagaaaaaac
     3841 agtgctgaca gatatcaaga tcactaccaa agatgacgaa tatattattt caggaaaaga
     3901 aaataaaaaa gaaacaacag tcatatttac aaaaatcggt aattaccgtt tgaaggatga
     3961 ggatggcaac atctacagtc tatagtgccg tctgttggat cttcttttgt ttctcccctt
     4021 cttttctgct atagtaaaga agcaagatga ttgtaaagga gacgaaatgg atgatcgata
     4081 gtcctgatta ttgtacatgt aataacaaca aaaatatcca tacagaattt aatgaccaag
     4141 gaagcttgag catatgcgac aactgtcaca aactagtcaa agattcattt aaaacatttg
     4201 aatcttatcc tgttcaaaat tatgtaaaaa gtagaagaag ataaacaaat ccaagcaaaa
     4261 ctcactatgg ctgattttta tgaacttcaa actcagcttc ttcctttgat caagcttcat
     4321 cagtaaaaca aacagtggat catttcagtt tacaaatcaa agagtaaaga tttatttatt
     4381 tcaaatattt gaatataaaa taaccaaacg atagaaaggc ttattctaca tcacgtagta
     4441 atttttcttt cccgtcgttt ggttttttat ttttagccta attcttgacc ttttgcatgc
     4501 tggttatccg taaaatattt tccagcaggc tttccttcca attcagctaa tttttcttgg
     4561 atctgacgga tcaccttttt tgcatcttct ggattttcta caaaatcgaa tttatcccca
     4621 tccacttgta ttttaggaca tacatcaaaa tcttcatacc attgattata gcgggtgttc
     4681 aacgtttcat aataggtata taacgtctca tcgctttcta gctgttcata gtcacgccca
     4741 cgttttttga tacgttctag catcgtgtcc aaagaaactt tgatatggac catcaaatca
     4801 ggccgttttt ttggagcaac atcatctaac tcgtttagca tcgtatctaa taagctatcg
     4861 tactgctgta cttcgatatc cgttacccga cctaaatcag cgtttagatg gaacaacagg
     4921 ctatcttcat agatggatcg gtctagcaca ttgtcatcgt tcactaaagc atttttcatt
     4981 gctaaaaatc gcttgttcaa aaagaagatt tgcaataaaa aagcgtactg ctcaggattt
     5041 gaataaaaga gcggcaatac ttcgttatct tcgacatttt cataaaatgg tttagaatca
     5101 atctcttttg caagcatatc cgtcaaactg ctcttacctg caccaatcgt tcctgaaatc
     5161 aaaatcactg acatacaacc cttcctcttc tataagtact cgcctacgta agtttcaaca
     5221 ggcaatgttt caaaatcata ttcacgtaca gcttggtctg attttaaata cgtatggaga
     5281 ctttgtgtaa tcatcaagtt cgtaggtacc gtctcttctt gtacaatgat catcggtttt
     5341 ttcaacatag ttgccacacc taattcgtaa gccgttcctg gatcgattgt ttgatgctca
     5401 aagtcaagga tagcaacaac aaattctgca tttgtcagtt cttccatatc tttttcgtat
     5461 acttcctgcg cccatccagc gctgaatagt tcataattgc tttcttggtg atgacgtgga
     5521 gagtagaaac ttgtgactgt tttgttttcc tccaacgctt tttcgatacg acttactcga
     5581 tcgatttgct cctctgaaaa aaatggtcct gctaaatata tttgactcat tattttgcct
     5641 ccttcaataa taactgattt ttaatagata ctgctcgtaa ttcattgacc agttccaatg
     5701 gattgttgga aaagattact tgatccaacc attttttatc cataaaatcc tcttttgcaa
     5761 aagtttctaa taattgttgc aagccgctgt aataaccgcc aatattcaat attccgattg
     5821 gcttgtcggg cgtcagaccg attgctatcc aagaaagaag ttgtgagaat tcctccaatg
     5881 tacctagacc tccagggaaa acgaggacag catctccctt ttcaaataat aatacctttc
     5941 tttcatcgat cgtttctgta ggaataaatg tagtaacatc ttttccaggc aattcagttt
     6001 caaaaagccc attcggatag atacctgtga cttttccgtt attttccagt actgttttac
     6061 taatcactcc cataatcccg gagtatgaac caccgtaaac cagttcgatt tcttcttgtg
     6121 ccatgtattt tcctaacgtt tgcgcaataa atttgtaact ttctttatta ccaaaacggg
     6181 aaccgcaaaa aacagtaagt ttcataaaaa aacactccct aactcaaaag tctatcttca
     6241 ttctacacgt gtttttttaa aataaaagac tagattttgt agttttttag tggtatatag
     6301 gatagacgcc actatatata gtatttagaa aaaaatcatt tttcagccat tttatagata
     6361 aagcaaaaat aaataacgtg ttttcatcga tatgaaatga cttacttata gaagatactg
     6421 ggatttcgtc ctatatgcca ataacgtgta ttattttaaa caacaaatac ttgtttcttt
     6481 tattaacgaa aaaatcattt tgttctcgtg ttatgtttca ctgataaatg atgtttctct
     6541 aaataaaaaa gccgacgaac taaacgatca aaatttgatc atttttgttc atcagcttct
     6601 cctaaaaatc tttttgttaa cgataaatag ctggatttgt aaaaatatca cttgaatcgt
     6661 cactgctgga agaaaattca gtgataaccg cacctgttgc gcctgcctgg aactagtgtt
     6721 tgatattggg ctcgaacgtg tactgatctc tagcatgaag aataatttct ttttttactg
     6781 tataataact ttctttcctt tttggaatag tacaggaaag tttttttgtt tcctttcctt
     6841 cgacataagg tataccagac cctttcgaca acgaaaggtt ctcatttttc cttcatcgtc
     6901 atttcaagga gggtgccgat gctctggaca gtttcgatcg ctcctaaacc aaaatctgta
     6961 tattcgattc tttctaactc tgattctttc ataccattcc tgtctcaagg tacatctatt
     7021 gtactttttt cttgtaatcc attctgcctc tcctaactaa ttgtcaaatc atcccaagat
     7081 atgtcttctt cctctttttc ttcttggatg atttctgcat tcggatctac tggttttttc
     7141 aataataaaa tggttattcc tgtgacaaaa gaaccagcta acaatgcacc aatgaatgcc
     7201 catggtctgg tcatcgtagc gactgcaaac attcccgatg ccggaatacg gctatctgcg
     7261 ccccaaaaca tgcttaatcc gccacctact gtcccaccga ctcccgttgc tatgacattg
     7321 cgtgccaagt cgttcaatac gatagggaaa catccctctg tgatctgaca gatccccata
     7381 ggaaaagcgg tcttcaatgt atcgatttct tgacgattga gtatattttt acgaatgatt
     7441 ttccctaaga aataagcgat cgtcaagccg aatggggtta ccatcgaagc taaaatcaat
     7501 acagtgattg gttcatagat tccttctgaa aatcgtgcca caccccctta tccagaataa
     7561 atggtaatcc agaaacagcc gtgtatctgc ggtaataagc c
//
LOCUS       DBNICI010000130         7598 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.131, whole genome shotgun sequence.
ACCESSION   DBNICI010000130 DBNICI010000000
VERSION     DBNICI010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.131"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(136..426)
                     /locus_tag="AC1ZCC_002233"
     CDS             complement(136..426)
                     /locus_tag="AC1ZCC_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630461.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(544..804)
                     /locus_tag="AC1ZCC_002234"
     CDS             complement(544..804)
                     /locus_tag="AC1ZCC_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630462.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(941..1354)
                     /locus_tag="AC1ZCC_002235"
     CDS             complement(941..1354)
                     /locus_tag="AC1ZCC_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIQ1630463.1"
                     /translation="MANVPSIRDMFPDFNDVFSPVFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1810..2295)
                     /locus_tag="AC1ZCC_002236"
     CDS             complement(1810..2295)
                     /locus_tag="AC1ZCC_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630464.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2434..2649)
                     /locus_tag="AC1ZCC_002237"
     CDS             complement(2434..2649)
                     /locus_tag="AC1ZCC_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HIQ1630465.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2873..3673
                     /locus_tag="AC1ZCC_002238"
     CDS             2873..3673
                     /locus_tag="AC1ZCC_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIQ1630466.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3692..5560
                     /locus_tag="AC1ZCC_002239"
     CDS             3692..5560
                     /locus_tag="AC1ZCC_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HIQ1630467.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYNILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5553..6044
                     /locus_tag="AC1ZCC_002240"
     CDS             5553..6044
                     /locus_tag="AC1ZCC_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HIQ1630468.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6032..6586
                     /gene="srlA"
                     /locus_tag="AC1ZCC_002241"
     CDS             6032..6586
                     /gene="srlA"
                     /locus_tag="AC1ZCC_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015795 - sorbitol transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HIQ1630469.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6604..>7598
                     /gene="srlE"
                     /locus_tag="AC1ZCC_002242"
     CDS             6604..>7598
                     /gene="srlE"
                     /locus_tag="AC1ZCC_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015795 - sorbitol transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HIQ1630470.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2709 a         1213 c         1441 g         2235 t
ORIGIN      
        1 tgttactatg aataacataa taagaaagta aagtggattg atttgagatc tttttatact
       61 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
      121 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      181 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      241 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      301 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      361 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      421 ccccacattc aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt
      481 atgaataaca gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga
      541 agtttatttt tttctgaaat attctaacac aaatatgaaa aagatggccg aagctaataa
      601 gccgatttcg tagcaaaaca atgagatacc agagtcgtaa taataaaatc ctctaatctt
      661 atggataaaa tagatcatcg gacaagcaga taagagtaag tatttgttag gccttttgaa
      721 taagtaaaag aaagcactgg tcattatgat aaatggtatc caaaacaaaa gtttacttaa
      781 gacaatcaaa tgatcaactc ccatattttt taccaagaga gatcataaca tctttatcgt
      841 aaaaacaaaa tatttttttg ctcattttga aaatttaaac atactcgttc ttatccaaat
      901 atataaaaat cgcccctcat tcatcgagag gcgataagtt ctaatttaac tcaatttttt
      961 ttgtttcttt tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa
     1021 aggttccagt gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga
     1081 ccgaatgtct ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat
     1141 gattggcgga gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac
     1201 agccaggcat atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg
     1261 gataacttga aacacctaaa aaatcattga atactggact aaacacgtca ttgaaatcag
     1321 ggaacatgtc gcgaatgcta ggtacatttg ccatttttga tcatcctttc aataagtatt
     1381 ttttatttat ccctatttac gtaataactc tattttgaaa aaaatgcaag tataatggtt
     1441 agctttgtga atatttcatc taatacgata caaaggctat ctttctaatt tgaaaaaggg
     1501 atcattaaaa taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca
     1561 aatatagaaa aattgaatct agaaaaccgc ccgacaataa atttcattaa ctcagttgac
     1621 cgaaaatgaa gtagatacca aaaatttatc aagagagcaa gtagagaagt gagtatattc
     1681 ataagagaaa tagctgtaac gaattattag gttaatgcaa atgcttattt atactccgta
     1741 taactttctc agaaaaaatg ataccaataa tgacttgcta gtactgtaaa tccgtataat
     1801 atcatctaat caaaatggtg ttccatttgc atctgtcatc gtaatcgatc cttcgggtga
     1861 cactcgataa aaaccaacgc ttccagatcc tccttgggaa cggatttgtt tgctggaagc
     1921 tgtgaacaaa taatctcgat caattttttg ataaaagcta ataacaatat cgtcactttg
     1981 taattcggaa cggtgggcaa taagaaatag aagtgctttg tcagagtttt ctaatggaag
     2041 agaaggtaca gtgctttctt gcatttcaga tgttgaagaa atgctaaacg actgaatact
     2101 tgattggcta tgttgcgaat ctacattacc agtagaagta ttagtaatgg aagtagtaga
     2161 agtactttta gttgaaccaa cctcagttgt tgaaatggct gctgaagatt ctttaacaga
     2221 attctttgtt ttatatccac aggcagaaaa aagtattaca gttcccaaca agcctgcgta
     2281 aatcccttta tgcatgatag ctcctcctaa aatagattat ctttattcag tatatcactt
     2341 aaacaaagaa aagcagatgg tttaaaatga aaaaaccttc ccaataaagg gaaggttcat
     2401 gaagataata acttttttaa taattatagc tttctaatat ctttcatcaa cttttctagt
     2461 aatcattggg aaaaatttca agcagtagat cgcacaaata cctacgacga tataaatgat
     2521 cttcgcaaaa atagttgttg aaccaccagc aatcatagct actaaatcaa gttcgaacaa
     2581 gcctaccaac agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa
     2641 agttttcatt agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa
     2701 cattgcttat ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt
     2761 tgctcttcca ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt
     2821 ttagtgagct tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga
     2881 ttggttggat attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag
     2941 aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga
     3001 atgccgtata cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga
     3061 catagaagaa gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa
     3121 taatgcagga ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta
     3181 tgaattatca gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt
     3241 gatgagccaa gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct
     3301 ttcttctgaa tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc
     3361 agcgatgaac agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt
     3421 ggtaggtgtt gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga
     3481 agctctttct tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac
     3541 aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta
     3601 ttatctttca gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa
     3661 aacaagagga taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt
     3721 attgaaatta ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa
     3781 aaccagtaat caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat
     3841 tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt
     3901 tgatgagatc atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag
     3961 aatcgcttat ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt
     4021 gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga
     4081 gatgatcaat gattttgatg taaagattac aggcacacca aataaaggac taagaataat
     4141 aggagatgag ctagaattac gactactttt attttatttt gtttctccct acttttcaga
     4201 gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac
     4261 gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag
     4321 aatctctagt aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata
     4381 tatccaagaa ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag
     4441 ccaatatgaa aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat
     4501 gataaggaaa gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg
     4561 acgattagat ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct
     4621 ctctttattg ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga
     4681 gatagaaaaa aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga
     4741 agctatcttg caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga
     4801 gttagcattt cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac
     4861 gacaggaaaa ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga
     4921 tatacaaata acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt
     4981 cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt
     5041 gactaatctt tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg
     5101 attagccggg tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta
     5161 tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga ggctattatg tggatgaaga
     5221 atttgaaaaa aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt
     5281 tgcttttcct cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat
     5341 aactgatcaa ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc
     5401 tgaagaaatg acggctgaag ttgagaatga attatttgag ctatacaata tcctatttga
     5461 actcataaaa atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt
     5521 aaaactaatc aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt
     5581 tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca
     5641 atcaaacata tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga
     5701 aaatccgttc aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag
     5761 tcagaaaaat gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg
     5821 gtgaagatat ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga
     5881 cacaagcagc tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa
     5941 aaagtgaaca accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa
     6001 gtacgatcaa agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg
     6061 atttatgagg ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt
     6121 accagttgtc ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga
     6181 ccgagtaacg aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt
     6241 accgttctta tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct
     6301 tccagaatac tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa
     6361 tggtgtattc ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg
     6421 gatcgaaacc ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact
     6481 gctgatgaac tttattggtg gttggataac tgattttact actggatttg tatgcagaca
     6541 gcaaggaatc gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt
     6601 taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt
     6661 attaggccga caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca
     6721 atgatcgtag ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca
     6781 tcaatacctg atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt
     6841 ggaatttatc ctaaaaaagg gataccaacc atcaatatcg tttccacagg aaaaagtggc
     6901 ccattagcac aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata
     6961 cagccactaa tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa
     7021 aagtacaaat atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg
     7081 atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga
     7141 gaagcagtag atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg
     7201 attggtgtga tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta
     7261 gcaggaaatg cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct
     7321 cctttgcttg gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag
     7381 attgggaaag gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact
     7441 caaaatgcat gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca
     7501 gtagaagtag gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt
     7561 atcattgctt gggtagcaag ttttggatta tatagctg
//
LOCUS       DBNICI010000131         7510 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.132, whole genome shotgun sequence.
ACCESSION   DBNICI010000131 DBNICI010000000
VERSION     DBNICI010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.132"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(2..2167)
                     /locus_tag="AC1ZCC_002243"
     CDS             complement(2..2167)
                     /locus_tag="AC1ZCC_002243"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HIQ1630471.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            2427..4721
                     /locus_tag="AC1ZCC_002244"
     CDS             2427..4721
                     /locus_tag="AC1ZCC_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HIQ1630472.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            4714..5379
                     /gene="pgmB"
                     /locus_tag="AC1ZCC_002245"
     CDS             4714..5379
                     /gene="pgmB"
                     /locus_tag="AC1ZCC_002245"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HIQ1630473.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            5372..6379
                     /locus_tag="AC1ZCC_002246"
     CDS             5372..6379
                     /locus_tag="AC1ZCC_002246"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIQ1630474.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            6401..7420
                     /locus_tag="AC1ZCC_002247"
     CDS             6401..7420
                     /locus_tag="AC1ZCC_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIQ1630475.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
BASE COUNT         2340 a         1639 c         1410 g         2121 t
ORIGIN      
        1 cttacatttt aatggagccg atcactttac cagctgtcac tgtacctaat tgcttgatca
       61 ctacttcttc tactttatcg tttgtaaata cgatcatgat cgttggatct ttttcttcgt
      121 tttggatcac atcaagatcc atctcagcta atttcgttcc tgttcctact tcttggcctt
      181 ctttgacagc aatctcaaaa gctggctgat tcatttggac tgtatcgatt cccatatgga
      241 tcaaaacttc gataccatct ttcgtttcca gcccaattgc atgtttgcta gggaaaatac
      301 ttgtcacttt tccagcgatc ggcgcaacaa cttctcttga tgttggaata acagcaaagc
      361 cgtcacccat cattttttgt gagaataccg gatcttttac ttcagataaa ggaatcactt
      421 ctccagaagc tgcggaaact agttcttgtt ctatacctaa gtattgctta ctacctgctt
      481 gtccatttcc tgtagaaacc atttctggct ctggaatctc tgctcctgat tgcagcagat
      541 cttcgatatc tgatttcaag acatctgctt ttggtccata aactgcttgg actccattgt
      601 ctttgacgat caatcccatc gcccctgctc gtttccaggc ttcttcagtc cctacttttt
      661 ctcgatcatt tacacttacg cgtaaccgag tcatacaagc atcgacatct ttgatatttt
      721 cttttcctcc cagcaaatga atgatctgga ccacttgctg atctgctgtt cctgttccgc
      781 tgttagatac aacggtttct tcactgtcat tgtcgtaatt tccgttacgt cccggtgtag
      841 caaagttgaa ttttttgatc aagaaatttg ctaagaaata cgtaacaaca ccaaaagcga
      901 tcacacacaa cacgaagttg ataagatctc cacctagacc cgctttgata gctaacggtg
      961 tccgagtcag caattcgata ttcccaaatg aatggacccg caatggcaag atatctgcca
     1021 tcgcaaatgc agccccttgg atcactgaat aaatgacata taatggcaca gcagcaaaca
     1081 taaacatgaa ctccaatggt tctgttacac ctgttagaaa gacagccaac gctgctgaaa
     1141 aatacattga tttatatctt gctttcttat caggatcgac attacgatac atcgcaaaag
     1201 ccattcccat caagatacct gaagaaccaa tcatttgtcc tactttgaaa cgagccggcg
     1261 tccagttatg caacacaaac tcatattgcg acatatcccc tgcaccttta agattcacta
     1321 aatcagtggc ccaagccaac cataacggat cttgcccaaa tacttgtgtc cccgcctgtg
     1381 cgcctgataa aatctcatac gatccgccaa gctgtgtata gttgatagga atcgtcagca
     1441 tatgatgcaa tccaaatggc aatagtaaac gttctaatgt tccaaataaa aacggtgcta
     1501 gaatcggtgc gctttcttgt gattctgcaa tccataaacc aaaattattg attccagcct
     1561 gtacgtttgg ccacaccaca gcaagaatca atgccacgat tgttgaccat aagataacaa
     1621 caaacggaac aaagcgcttt ccattgaaaa atgacaaagc atccggcaat tttctataat
     1681 tatagtactt attataagcc attgccccga caaatccagc gatgatccct acgaatactc
     1741 ccatattcaa agctggtgct tccaatacac ttgtaaaaaa tcctttaacc atgatcttcg
     1801 taccgaacag agtgtgggta aatgcatctt cattcgcaag catgtcactt gtgacaccaa
     1861 agatagcccc agtgatccga ttgattaaaa taaatgagat cccagctgca aatgctccgc
     1921 ctgctctttc ttttgcccaa cttcctccta ttgctaaagc aaataaaata tgaagattac
     1981 caataattgc ccaaccaata ttttcaatta ctccgcccgt tgtgacaagc ggcgtccaat
     2041 caggattgat caagggaatt gatttcccta tactgatcat caacccagct gccggcataa
     2101 ctgctaccac aaccattaaa gcctttccaa atttttgcca aaactcaaaa ctaaatatct
     2161 ttttcattcc gtttagctcc tttacgcaat cggtttcgtt aaacgtatta tagcgtgcgt
     2221 tttctttttt gtaaatgctt tattttttat ttttctgtct ataccattct ataaaaccta
     2281 taatacgtgt gttcccttct ataattttct cctaaaaatt tattttttta agcttttttc
     2341 tatgaaatat tttcttttgt ctattgactt gtgcaatcgg ttgcgtttat tatgaacgta
     2401 taaaacagct cattaggagg agaaaaatga aacagatcaa acgactattt gctattgatc
     2461 catggaaaat cagcacgaca caattagata aaaaaaatct tcgactgcaa gaatctttaa
     2521 caagtattgg aaacggttat atggggatgc gcgggaactt tgaagaaact tactcagggg
     2581 accaccatca aggtacttat ttggcgggtg tctggtatcc cgataagaca cgtgtcggct
     2641 ggtggaaaaa tggttatcca gaatactttg gaaaagtgat caacgccttg aactttatcg
     2701 cgatagatat ttcgatcaac gatcagctga tcgacttggc ttctttggaa ccagaagact
     2761 tttactggga attagatatg aaaaatggtg tattatctcg tacattcaca atcactactt
     2821 ctactaataa agtacgcatt tccttcgaac gttttctgag cattgtcaaa aaagaatcag
     2881 cttatatcta tttaactata gaaatgcttg aaggtagcgg aacagtcaaa ctcgtatcaa
     2941 aattggatgg aaatgttcaa aacgaagaca gcaactatga tgaacatttt tggaatgaga
     3001 ttgatcgtgg aactatagaa gaaatcggct atttgacgac taaaactatt ccaaacaact
     3061 ttgggattga acaatttact gttactgcag caatgcgcca tttcgttaat ggaaaatcag
     3121 tcatccctga ctatcacgaa gatactttag cattacatgg ttgcttgtct gcagaattat
     3181 ctcaaggtga gacatggtca gtggaaaaag aaattatcgt tcttactagt agagatattc
     3241 cagaagcgca gcaagcagaa aaagctgtct cagaattaac gaaccagaca aacaattata
     3301 tccaagcaaa agaagagcaa acagcggctt gggcagaacg atggaagctt gccgatgtgg
     3361 tcatcgaagg agacgaagaa gcacaacaag gtattcgatt caatttgttc caactattct
     3421 ctacttacta cggcgaagat gaacgattga atatcggccc caaaggattc acaggagaaa
     3481 aatacggcgg agccacttac tgggatacag aagcgtatgc tgtccctctt tatcttgctt
     3541 tagccaaacc tgaagtaact aagaatctgc tgaaatatcg gcataatcaa ctgccgcaag
     3601 caatccacaa cgctcagcaa caaggattaa aaggcgcgct ttatcctatg gtcaccttca
     3661 ctggtgtcga atgccataac gagtgggaaa tcacattcga ggagattcac cgaaatggcg
     3721 cgattgccta tgcaatctac aattatacga attacaccgg cgatacggaa tatttgaaaa
     3781 atgaaggact ggaagtttta gcagaaatcg cacgcttctg ggcagaccgt gtccattttt
     3841 caaaacgagc aaataaatac atgatccacg gtgtgaccgg cccaaacgaa tatgagaaca
     3901 atatcaataa caattggtat acaaatacga tcgcagcttg ggtcttgcgt tacacgcgag
     3961 aaaattattt aacttatcaa aaagaagctg ctatcactat cagcgaagaa gaactagcca
     4021 aatggaaaga tatcgcagag aacatgtact tcccagttga cgaagaatta ggaatctttg
     4081 tccagcatga cacattctta gataaagatc tcatgcctgt atccgatatt cccacaagcg
     4141 atcttcctct gaatcagcat tggtcttggg acaaaatcct tagatcttgc ttcatcaagc
     4201 aggcagatgt actacagggg atttatttct tcaatgatac ttttacaaaa gaagaaaagc
     4261 aaaaaaactt tgatttctat gaaccaatga ctgtccatga gtcttccctt tcgccaagta
     4321 tccattcgat tttagcagca gaattaggaa tggaaagcaa agctgtagaa atgtaccagc
     4381 gaacagctcg cttggatttg gataattaca ataacgacac agaagacggc ttgcacatca
     4441 cttccatgac tggtagctgg ttgtcgattg tccaaggatt tgctcaaatg aaaactgccc
     4501 atgatcagtt gagattcgca ccatttctgc caaaaacttg gaaagcttat tctttccata
     4561 tcaattatcg cggacgttta ttgttcatct cagtcacaga tgaacaagtt actttcgagt
     4621 tattagaagg ttctcctcta gaaatttacg tctatgaaca gtcttattct ttagcggatc
     4681 gtctagaaat aacgctaaga aaggaggaag caaatgttta aaggggtatt gtttgattta
     4741 gacggtgtca ttaccgatac tgctgaattc cattatcgtg cttggcgaaa gcttggtcag
     4801 gaaatcggca ttacgatcga ccacacattc aacgaacagc taaaaggcgt cagccgagaa
     4861 gactcactct ctttgctttt agcttatggc ggaaaagaac actctttttc aaaggaagaa
     4921 tttgcagaat tagcgaaaag aaaaaacgat tattatttag agatgatcca gacgatcgaa
     4981 ccaaaagatg tcttccccgg ggtcgtgcct ttactggatt ctttaaaaga agaaaaaatc
     5041 aagattgctc ttgcttctgc aagtaaaaac gggccattcc tacttgaaaa aatgggtctt
     5101 acaccttact ttgatgctat tgccgacccg gcagaagcaa caaacggaaa acctgcgcca
     5161 gatatcttcc tccttgcagc aaaagctgtc ggactaaaag cagaagaatg tatcgggatc
     5221 gaagacgcgc aagccggtat ccaagccatc ctctcaagtg gcgctcaacc aattggcgtg
     5281 ggacgtgctg aagaccttgg agaggagatc ccgcttgtcc ctgatacgac ttttcttacg
     5341 atcgattact tgaaagaaaa gtggcatgac catggataat ccagagatct ccgtcgtacc
     5401 agttccagag actgaaataa agaaaatcac attgaagaac gactacttag aagtcgttct
     5461 tctcaattac ggtgcccgcc tgcaccaaat cttcgcacca gacaaagaag gaaaatcaga
     5521 aaatgtttta ttgtcttacg atcgtttcga agatgttcta acagacaaaa gcttttttgg
     5581 agcaactgtc ggacctgtag ccggaaggat ccgggatgct tcttggggtg agcatagatt
     5641 ggagaaaaac tgcggatcac accatatcca cggtggcacg aaaggctggt ctttccaatt
     5701 ttgggaagta gaagttttca aaactccatc atcaataggt gtggtctttt acttaaaaga
     5761 tgaattttct acctatccag gaccaatcac tgctacgatc acttaccgtt taacaaaaaa
     5821 cgaattagag atgacaacct ctgctagtag ccttgttgaa accatctgca atccgaccaa
     5881 ccatgcctac ttcaaccttt caggaaatgg aacaagagac atctatgaac atcagttaac
     5941 ggtcttttta gatggtgtgc ttgaattaga tcaagaaaaa ctgcctactg gaaattggac
     6001 aaaaaaagaa aaactgccaa tcgactttag aaaatcccca acactgcaag agatccttgc
     6061 atgttatcca gatggactag atgatgtatt tcttttgcat catcctaggc tttccaaaac
     6121 ttccttacaa ttattcgaga agcattctgg tcgccaaatg actatagcaa cttctaataa
     6181 aagtatggta ttattttcta cgactggatt cgaagctgat ttctctgtca atggaaaaca
     6241 gatgcattca aactacggat tagcaatcga accgcaagaa atccctgata tcgttcattt
     6301 ccccaaattt ggatcgatca acctccatcc tggtcaagaa cggatcagtc agacgattta
     6361 tcggttctct gcacaataac aagagaaagg tgtttttatc atggctatta ccgtaaaaga
     6421 tgtggcgaaa aaagccggtg ttgccacctc gaccgtctct cgtgtcatca acgatcatcc
     6481 tagtatttcc gagacaacaa agaagaaagt ccgaaaaatt atggatgaac taggttatgt
     6541 tcctaacatg actgcccgca atctaggaaa acgcatttcc agtgccatcg gcgtaatctt
     6601 accccctctt gattcaaaag agcggatcgg taacccattt tatcttgaga tcatggaagc
     6661 aatcaacgaa gaagcgcgga attatgatat gactacagca atcgctactg ctaaaaattt
     6721 tgaattgctg ttagagaacg tccagcgtat gcatttacaa aaacaagtag atggttttat
     6781 tctagtgtat tcagatagtc aagatactgt cattgattat ttataccaaa accacatccc
     6841 tttcactttg atcggacagc cttatcatca tgagaacgaa atcgtttatg tagataatga
     6901 taaccagctt ctaggtaaac aagcaacaga atttttgatc cagaatggac ataagaatat
     6961 cctgtttgtg acaaacacga ctcatgaaag tttgtatttc gaacgatatt tcggttatca
     7021 aaaagcaatg atgatggctg gtcttcctgc gttatccagt gtagtactag agcatccgga
     7081 ggattatctg tcctttgaga aaacgttgaa agaaaacgaa gccacagcat tggtggtcat
     7141 tgacgatatc ttcgcccttc gcacgatgca gttggcgcaa atgtatggct atgtcattcc
     7201 agagacactc tccgtcatta gcttcaacaa ctcgatcttc tcaacattaa cacaccctta
     7261 ccttacaagt atcgatatcg atatctccga attaggacgt gtcgctatgc aaaagctgcg
     7321 cgaattgatc catgaagaat tgagcagtgg cgtacgtttg gtcatccctc atcgcttgat
     7381 caaaagagag acagtcgttc ctttagaaaa cccatactaa atagctgact cactaaatac
     7441 gctgaccatt tatcctatgg tcagcttttt atgtaaaaga agatactact tctaacacat
     7501 tgtttatctg
//
LOCUS       DBNICI010000132         7478 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.133, whole genome shotgun sequence.
ACCESSION   DBNICI010000132 DBNICI010000000
VERSION     DBNICI010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.133"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(199..1890)
                     /gene="argS"
                     /locus_tag="AC1ZCC_002248"
     CDS             complement(199..1890)
                     /gene="argS"
                     /locus_tag="AC1ZCC_002248"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIQ1630476.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     misc_binding    complement(1967..2164)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2310..3257)
                     /gene="arcC"
                     /locus_tag="AC1ZCC_002249"
     CDS             complement(2310..3257)
                     /gene="arcC"
                     /locus_tag="AC1ZCC_002249"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HIQ1630477.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(3372..4391)
                     /gene="argF"
                     /locus_tag="AC1ZCC_002250"
     CDS             complement(3372..4391)
                     /gene="argF"
                     /locus_tag="AC1ZCC_002250"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIQ1630478.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(4482..5711)
                     /gene="arcA"
                     /locus_tag="AC1ZCC_002251"
     CDS             complement(4482..5711)
                     /gene="arcA"
                     /locus_tag="AC1ZCC_002251"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HIQ1630479.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            6172..6873
                     /locus_tag="AC1ZCC_002252"
     CDS             6172..6873
                     /locus_tag="AC1ZCC_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIQ1630480.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         2133 a         1528 c         1292 g         2525 t
ORIGIN      
        1 agggagctgt gacacgactt ttgtcacagt ccccttattt gcaaacaaac agtgtttaag
       61 agacaacctc tccactaaac ggtgttcatt cagctgactc tcggtatttg ctcaaaaaac
      121 gataaaattt gaaaagctat tttctctttt gtttctctaa tactcggatc agaacgctga
      181 tttcacattc tctttttctt acatcttatc tggtgcatga agtcctagca aacgaaggtc
      241 ttctttcaat aatacagtca ctgcataaac tagagctaag cgtgcttctt tttgttcatc
      301 atcagctaaa atcttcgtat gagcatagta tttgttgaat gcttgtgcta atttgattgc
      361 atgtttcgca atcactgacg gttcgtattt ttccccagca gacaagaccg tttcagggta
      421 tttttgaact aatttaacca cttcccagct ggtatcgtca ttcaatgcat agtctgctgt
      481 ttctgaaggg acaaatcctg ctttttccaa caggctgact gcacgagcat gtgtatattg
      541 cacataaggt ccagtttctc cttcaaatcg aacaacttct tccaggttaa agtcaaatgt
      601 attcagtcga tcgtttttca aatcgtggaa aatgactgcc ccaaccccaa cttgtttagc
      661 tacagcatct ttgttttcca aatcagggtt tttctcgctg atttgttctt tagcagattg
      721 gattgcttca tttaatactt cttctaataa gacgatcttt cctttacgag ttgataattt
      781 tttaccacct tgcgtgatca aaccaaatgg aatatggtgc atgtcgtcag accagtcaaa
      841 gcccatttcc ttcaagacag ctttcaactg tttaaaatga tagctttgtt cattccctac
      901 tacatataga gattgtttga agtcgtaagt acgtttacgg tacaatgcag cagctaagtc
      961 acgtgtaatg tatagtgtcg caccgtctga cttcttaatc aacgctggat tcaggtcata
     1021 agcagatagg tcaacgatct ctgcaccttt atcctcgttc agcaaatgtt tttcttctaa
     1081 aagtttgacg atttcatcca tcttgtcatt gtagaaagct tcaccgttca acgaatcgaa
     1141 acgtacttcc agcagatcat agattttatt gaattctttc atcgattcat cacggaacca
     1201 ttgccataat tgaatagcct ctttatcgcc ttcttccaga cgtttgaacc aagcacgagc
     1261 ttcttcattt aattctggtt ctgtttcagc tacttcatgg aattggacgt ataaacgaag
     1321 caattcattg attggttctg ctttaacagc ttcttctgta ccccattttt tgtaagcgac
     1381 gatcagtttt ccaaactgtg ttccccagtc acctaaatgg ttgatgcgga ttggctgata
     1441 accgattttt tccataatga aaccgatcga attaccgata actgtcgaac gcaaatgtcc
     1501 cattgagatt ggtttggcaa tattaggaga agacatgtcg attggtactg ttccttgatt
     1561 cccgatgttg ctatccccat aatgttcttt ttctttcact actgtttgca aaactttttt
     1621 gctgataagt tctttattca tgaaaaagtt caagtaaggt ccgacaactt cgattttttc
     1681 aaagttggca gaatcgattt tttctgctaa atctgcagcg atttgctgtg gcgcttttcg
     1741 gtaaactttt gctaatgaaa aggctggaaa tgcaacatct ccgtgttcag ctgattttgg
     1801 attttctagt aattgctcga cttgttccag tgttaaatca tctttgacag cgtcatagac
     1861 agcttttgcg acaagatctt tattattcat ctttattcct cctaaagtgt tcccttgatt
     1921 gttaaacaaa aaaaatctct agcgacggca tacttagtat gccatcacta gagacgagat
     1981 ttcccgcggt accactccaa ttgtcctaaa aaaggaccag ctccatctga tatcggtaga
     2041 cgaccgtctg tttaaaacag aattctccaa gtgcgcttcg ttgtatcttc ttcattcggc
     2101 ttccaccact tccgaattcg ctagccaaga atgatacaag tactctcttg ttcaacaaat
     2161 cttagtctta ttaaactttc ttgaattata tcagaaaaaa aaacgaaagg ctagtcgttt
     2221 cataaaaaaa caatccagat ggcctaaagg agaagaaata ccacctggat agctataaat
     2281 catttcgtaa atacgaaaga cttaacaaat taatcagcta cgataatcgt tccttcttca
     2341 gaagctaaaa gattctcgat attttctaaa gaagtgatga ttgccttagc gtttggtttt
     2401 gcttcaacaa atgcaatagc tgcttctact ttcggaagca tgcttcctgg agcgaattgc
     2461 ttttcatcaa tgtattgttt catttctgaa acagtaacag tttctaattt cttttgatct
     2521 ggtttttggt aattgacata cacattgtct actcctgtca atacgatcag caaatccgca
     2581 tcgatgattt cggctaattt ttctgaagca aaatctttat cgattacggc ttctcgtcct
     2641 tctaatccag tagctgtttc tacaacagga atcccaccgc caccaacaga gactgtgata
     2701 actccttgat cgaccagcgt ttcgatgaca cgtgcttctt taatcgagat gggtttaggg
     2761 gaagcgacta cttttctcca tccacgtcca gcatcttctt tgaatgtcac agtggagtca
     2821 gcattcattt gttctttggc ttcttcttcc gtgtagaatg gaccgactgg tttacttgga
     2881 tttttgaatg aaggatcatt ttcatccaca ataacttgag tgactaaaga aaccacatct
     2941 ttatcgatcc ctttttcctt cagtacttcg cccatggcgt tttgtaacca gtaaccgata
     3001 gatccttctg tcattgcaac acatgtatct aaaggcattg ccggtgtttt ctcagaatca
     3061 gcagcttgct gctgaatcaa taggttaccg acttgcggac catttccgtg ggagatgatc
     3121 aattcatctc cttgttcgat aaatttcact aaatactttg ctgtttccat taacgcttct
     3181 tgttgggctt ttgcacttgc atccgtagac aagatggcat tgccccctaa agcgacaact
     3241 acttttcgtt tggccatttc attcgtcctt tcttcagttc taccgaattt tctagataac
     3301 aaaacgagcc agcgttttgt gattttccct caatgctgac tcgttttttc gtgagatgaa
     3361 tgtggttatg cttaaacgcg agggataaac aagttaccta atgttgctgc catgatcgct
     3421 ttgattgagt gcatacgatt ttctgcttgg tcaaattgac gagcatattt gctgcggaag
     3481 acttcatctg ttacttccat ttcagtgatg ccaaagcgtt ctttcatttg ttcaccgtaa
     3541 acagtgtttg tatcatggaa agctggcaag cagtgcaaga agatcaaacg atctgtgttg
     3601 tgtgtttttt ctaccatttc catattgatt tggtaaggtt tcaacaattt gatacgttct
     3661 tcgaatttat cttcttcacc cattgatacc catacatcag aataaaggac gtctgctccg
     3721 tctacacctt tatcaacatc gtcggtaatc atcaattgtg caccggattt ttcagcaaag
     3781 ccttctgcca ttttgacgat ttcttcttca ggttgtaatt cttttggtgc aacgatacgc
     3841 atattcgctc ctaaaattgc acctgtgact aataaagagt tagccatatt gtttcgacca
     3901 tctccgcagt aagcaacagt gataccttct actgttccaa agttttcttg gatagttaaa
     3961 aagtcagcga tcatttgtgt tggatgccat tcatcagtca agccattcca aactggaaca
     4021 ccagagaatt ctgctagttc ctctaccatt ttttgactga atccacggaa ttcgattccg
     4081 tcaaacatac gtcctagtac tttagctgta tcttctgtgg attctttttt acctaattgg
     4141 atatcatttg cacctagata ttcaggatgt gcacctagat cgatcgctgc agtagtaaat
     4201 gcagaacgag tacgtgtaga tgttttttca aataaaagcg caatattttt tccttctaga
     4261 tagtgatgtg ggataccacg ttttttcaag tctttcaagt gttctgaaaa atcgatcaaa
     4321 tattgtaatt cttcttttgt aaagtctttt tctgctaata agcttcttcc ttggaatact
     4381 gattctttca tattaaacat tctcctttag attgttttta tttaagatta aagaggaaat
     4441 cccgacggca gctgacagta agactgccgt caggaaacaa attacttcaa gtcttcacgg
     4501 acaagtggtt ggctcatgca gcgtggacca ccacgacctc gtgacaattc acttgaattg
     4561 atttctaaca ctttgatgcc atagctacga agcaattcgt tcgatacata atttcggtta
     4621 taagtgacta caacacctgg agcgattgcc aatgtgtttg atccatcatt ccattgttct
     4681 cgaggtgcaa cgatctcatc tccattacca gttgggatca gtacaagatc atctaatcca
     4741 agagcagctt tcatcgtttc gtgtaagtta tctgaatgag taatcttgat atcatcacca
     4801 tctggttcga ttgtaaagac atccatctta ccgtctttac tttgaattgc tggatggata
     4861 gtgaatttat cgtgatctac cattgtaaat acggtatcca aatgcatcat tgcacggttg
     4921 ttcggaattt tgattgccaa cactttttca aatcctgaat tttttgcgaa taggtggcgt
     4981 gctaattttt ctaaagcttt cgcattagtt cgttgagaaa tcccaacagc tactacttta
     5041 tcgcttaaga tcaattcgtc tccgccttcg atatgatctg gattttcacg atccaaccat
     5101 acttcgattc ctttatttgc aaatcttgga tgatgtttca aaatatattc tgtaaacatc
     5161 gattcacgac gacgtgcttc aaaagtcatc ttattgactg tcatcccatt tcccattgat
     5221 gcggaagggt cgcgtgtaaa gtacaagttt ggcattgggt ccatgtaaaa tggatagtca
     5281 tcgtctgctg acagatcata tagactgcta gaacgtactt cgatatcttt tgtacgaacc
     5341 ccagccatga ttttatctac catttcttgc gtttccattg atagaaggaa ctcatgaaga
     5401 ccctctctta cagcattcga tgcaatatga gattcatcca acattttatt caagaattgt
     5461 tctttgacat tgcctgcgtc aatcgcttct gccgctaatt tttccaagta aagagtctcg
     5521 acgccttcat tttgcaatgt cttcgcaaag ttgtcatgtt cttcttgagc aatcggaaga
     5581 tatggaatat catcgaacaa cagacgatcc ataatatctg gtgtcaaatt ttccacttct
     5641 tgtccaggcc gtttcagtaa aactgtcttt aatttcccta tttcagagaa aacgtgaata
     5701 ggtttatcca tgttgtgttg cctccttctt tttgattaca tgtataggat atcctccttt
     5761 tgtaatcact ttcataattt tgtctcattc aaaatgagac attcctcaca ttaatattcg
     5821 ataattaaat aattaaacgt ttttctattt ttaagcgtaa tttattcatt ttttattctg
     5881 tatacacttt caaaaaaata tttattcatt gaaaaaattt ttttgaataa attaaaaaaa
     5941 taaattatat tatcgttgtt agcgtttact atatgtatta ttgttgtttt ttatacacaa
     6001 atcattcgct tcaaaacaaa ttattcaata ccattgtata ttttttacat aataacaaat
     6061 tatatttgta aagtattaaa acttgtttct aatgttggta tgttaaaact ttacaaattc
     6121 tttaaaaaaa gtgacaataa atatagtgaa caataaggag aggacttttt aatgcgtgtt
     6181 aataaaatcg attttcactt ttcaaaactc agatatcaac ctgattttga acatttctcg
     6241 gatgaagagt tccaacagat caaggaacat actgttcttc gttcttataa aaaaggacag
     6301 atccttttcg acgatggtga tcccagacaa cggttgtata tcttgaccaa aggactcgtt
     6361 cgtttagaac gttatgatga aagtgcaact tattactact atgactatgt gaatacaaat
     6421 aacctttttc ctatgtcagg aatgtttgga gaagagacct acacttatac agctcaagcg
     6481 atgactgata ttgaaacatt ctaccttcct gtttcctact atgaaaaatt agcacgaaaa
     6541 aatcccgaac agttgatcta tatgatacaa aaaatttcta aagtagttga aatacatgaa
     6601 ttgcgtatcc aaatcggatt gacctctagc gcctttgacc gggtaaaaca aaatttattc
     6661 attttgaaag aagaattggg cgtaccaaca aaagatggtc atatcaccat tccttatccg
     6721 atcaccttaa aagaattagc agtgaatagc ggcacgacac gtgaaactgc tggacaagta
     6781 atcaaacaac tacgtgattc cgggctgtta gattatgaca aaaagcgttt tactttctgt
     6841 aagacagcta ctactccagc tatgagagaa taacgcgaac aaaaataagc agtgaccctg
     6901 ataaatatca gggtcactgc ttattttcct ccctaaaact tctgaaagat ccggcagttt
     6961 tagatcactg gacaagactc aaagattttc tatgtgtgtt tcagttatgt aatattcact
     7021 gtttgcaaaa aatactgtta gattaactag atgaagcgtc ttgtcctcat ccgatctaga
     7081 ttattcgata ttcctcgaca ataaacgatt actcagtgag ggcaatcaga ggaacggata
     7141 atgataaata agctgtaaaa aatagcaaaa taacaataat gattaaaagt atgtaaaaaa
     7201 tagggctttg acaaaaacgt gtcacagccc tatttcgatt aaacggtgtg tacctgtcaa
     7261 ctcttcggtc acaagtcata atgttcataa acatgaggag ctgttttttg aaattgttcc
     7321 ttgtgaatca gagttaaacg acaggcttac gaactcttga ttaaacggcg ttcaaaagct
     7381 tgcatacgcg gtaataagaa cagccaggtc aaaaacatga gaagccgttt ttcctgggct
     7441 gttcttatta cttgatgcag ggcagctttt tcacaacc
//
LOCUS       DBNICI010000133         7463 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.134, whole genome shotgun sequence.
ACCESSION   DBNICI010000133 DBNICI010000000
VERSION     DBNICI010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.134"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            36..1177
                     /locus_tag="AC1ZCC_002253"
     CDS             join(36..264,264..1177)
                     /locus_tag="AC1ZCC_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HIQ1630481.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1412..1864
                     /locus_tag="AC1ZCC_002254"
     CDS             1412..1864
                     /locus_tag="AC1ZCC_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630482.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1878..2567
                     /locus_tag="AC1ZCC_002255"
     CDS             1878..2567
                     /locus_tag="AC1ZCC_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630483.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2692..5043
                     /locus_tag="AC1ZCC_002256"
     CDS             2692..5043
                     /locus_tag="AC1ZCC_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HIQ1630484.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5132..5518
                     /locus_tag="AC1ZCC_002257"
     CDS             5132..5518
                     /locus_tag="AC1ZCC_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630485.1"
                     /translation="MEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIVDYF
                     ANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKKKEY
                     NYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5843..6460
                     /locus_tag="AC1ZCC_002258"
     CDS             5843..6460
                     /locus_tag="AC1ZCC_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIQ1630486.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6501..6800
                     /locus_tag="AC1ZCC_002259"
     CDS             6501..6800
                     /locus_tag="AC1ZCC_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HIQ1630487.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6834..7001
                     /locus_tag="AC1ZCC_002260"
     CDS             6834..7001
                     /locus_tag="AC1ZCC_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIQ1630488.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7046..>7463)
                     /locus_tag="AC1ZCC_002261"
     CDS             complement(7046..>7463)
                     /locus_tag="AC1ZCC_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIQ1630489.1"
                     /translation="LDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDT
                     AMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVF
                     KTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2574 a         1124 c         1494 g         2271 t
ORIGIN      
        1 tagaatctaa ctactaagaa aaggacgtgt ccacgatggc aaaagccaag aaaaaatttg
       61 atgaagactt taagaagatg atcttagatt taaaccagtc tggccaatcg gtcgaagaac
      121 tggcagcgca atatggtatc gctacacaaa cgatttatcg ttggaagaag cttcatacaa
      181 agaatgtagc aaccggcatg actgaggcag aaatcctagc gatgaaaaag gaaatggccc
      241 gcatgcagga ggaaaacact atcctaaaaa aggctttaac catattcgct caaaagtaaa
      301 aatgagtgat gtcttcgaat ttattcaatc aaatgttcat gatcatgacg taaaacaaat
      361 gtgtaccgtt ttggaagcac cgaaatccag ctactatgac tggaagaaaa agaaaccttc
      421 caagcgccaa tcagaaaaca aacgcttaaa ccgcttgatt tcaggaatct actttgaaaa
      481 caagggtatc tatggtgcgc ctaaaattca taagattctt gttggccgtg gcgagacctt
      541 gtccttaaag aaagtacaaa tactgatgag aaaattaggc ttgcgttcag tcaccctcaa
      601 gaagtacaag cctggcaaac aagcgaaagt taaaagtgaa ggacgtaaaa acctgttaaa
      661 ccaagatttt accactacgt cgatcaatca aaaatgggtg acagatatta cctatattca
      721 taccctaaaa gatggctgga cctacctctc cactattcaa gacctccaca caaagaaagt
      781 catcggttgg aagtttggca agcagatgac gaaagaattg gttatcgaaa cccttgacaa
      841 tgcgctctta aaccaaacac cctccgagaa cttgatcatc cactcagatt tgggttctca
      901 gtacactagc gaagcctatg aggcaaagct gaatgaattg aacattcggc actccttcag
      961 tcgtaagggc tgtccctacg ataatgccgg tatcgaatcg tttcacgcct cactcaaaaa
     1021 agaagaagtc tatccatcaa aagcgtatga aaactttgaa gctgcttatt tagcgttatt
     1081 tcaatacatt gaaggatttt acaatcgcaa gcgaatccat agtagtatca actatttaac
     1141 accaaatcag atggaggagt tggcattgca agcttagttc ctagctacca cttacatttt
     1201 tttggcgaga tttccacagg gtttcaactt catcgcttcg atcagcggtc aaacggtttt
     1261 ggtttgaccg ctggtcgaaa cgatgagata ctcacccgct ggaatctcga aaaaattctc
     1321 acaaatcgtg tccgaggtat tgactcaaat ccaccaatta tcttatcgta gatatatatt
     1381 ataaatttaa taagaaagcg aggtgagaat cgtgaaaaaa acgatactaa gtattttttc
     1441 agtatttaca atattaattg ggggctattt tcttttgaat ttagattctg ttaattcaaa
     1501 cgctcaaata aaagatcaaa gagtagctga acataataat aaaaaaatga gtttgaccaa
     1561 aaatatctat tctatgtcag atgaagaatt gtataaagaa tttggtgatt tatccgggga
     1621 acagttagaa gaaagtattc caggaattag tgactcagat aaaattgttg tcttgtctgc
     1681 tggtgggcaa ttaatgggag atggcaaatc cgaattagtt agttataaac aaaatcacag
     1741 acagtttatt gatgttaata ctgattccaa cgctatttcg attaaggatc taaaagagct
     1801 tttaaatagt aaaagaaatg aattaaagca gataaaagaa actaattcag tggaattaga
     1861 gtaaaggaag attcattatg aaaaagattt ttggtgtttc tgttgggcta cttatgatgt
     1921 taacatttgg aaatgcagta tatgctgctg agaaagaagt tatttcagat ccagagcagt
     1981 acgcctcacg atactttgct gattttgaag aagaagataa agtacttgca ttgccagaag
     2041 gtggtttttt aaaaggggaa gctaaaattg tatctatgga taatccagat ataattcaag
     2101 gttcatataa ttcagaaact gatcctgctt cagtttcagt gacagaagca aaaaaaattg
     2161 ttgctgaaaa attcaaaaat gatattgaaa ctactccaat tatgcctcga ggagcatttc
     2221 cacccgatgt tggtactagc ggacaccttc ttgtactagc aggaggagga agctataaat
     2281 cacagccatt tagtggtgcg ggatggcgtt ttggaggtat gggtgtaaaa ccagctgatg
     2341 gtacaggcaa ctttttacgt tggaaatcct acattgacga tggaagagta ggaaatgtaa
     2401 atcaagccaa cgcaactaaa tctggttcta ttcaaggaat ggcattgtat gctcgtgaag
     2461 gattcaaata ctttgcacca aacgggcctg gttggacaat gaatataggt atttattaca
     2521 cttataatcc tgttgcaggt acgtattatg ttgttcaaaa tatttaataa aacagcacaa
     2581 agagatttct ttgtgctgtt ttattatttt tagttgttga caaacactaa aatataaaat
     2641 ataatcaaca cataaacgaa cgtatgttcg gcaagaaagc aggggatttt tatggatgag
     2701 atggttttaa gtactcagaa atggctaaac aagaaataca gtaatgttac agggtttgat
     2761 aaggttccag aaaatgggcg aacagggtgg cctacaatct atggacttat tgaaggacta
     2821 caagtagaac ttggtattac taatctagta gctaattttg gacctaccac tgaaaaaatg
     2881 tacgataatc aggttactcc taaatggggc aaaaatctac ctaaaaatat tgtatttctt
     2941 attcaaggtg ccttttggtg taaggggatt aatccaggtg gcttcgatgg ggtatatact
     3001 ccatatttag atactgctgt aaaagagttg cagactgatg cagggtttaa tggaagcgct
     3061 gtaactggag ttttggatgc gaaatgggcc aaagcgttat ttgatatgag tgcttttgtt
     3121 cttgttcccg gcggagatag taaaattaga aaaatgcagc aatggttaaa tgtaaattat
     3181 ttagaataca cgggaataat gccttgtgat ggtatttatc aaagaaattc aaatcaagca
     3241 ttaatttttg cattacaagc tgaactagga tattctccca gtgaagcaac agggtcttat
     3301 ggtaatggga caactagtaa aacttatcct gtgtctgaag gtaactcggg aaactatgta
     3361 agaataattc aatatggatt atatgttaat ggttattttg aagacggtac attcgatggt
     3421 attttcacta aatatatggg tttagaagta ctggcattcc gaaaatttat ggtattgcct
     3481 gaatatactg aaatagcaga tgttgttgta ataaaaggac tattgtctag cgctggagat
     3541 attagaagat ctgcagatgg cgcagatact tctacgcaat taactagatc tcaaatccaa
     3601 acattggttg ataatgatat aaaaatagtt ggtagatatc ttactggtac tgttggaatt
     3661 ggaaaagatg aacgaaataa gtatctaact acagaggaat tgaataatat tttttctaaa
     3721 aatttatctg tgtttcctat ctatcaagat ggaggtgcag cattagctta ttttacttat
     3781 gatcgtggct tatcagatgg taagaaggct atagatgcag caaaaaatct aaatatacca
     3841 ttaacaacag ttatttattt tgctgttgat ctagacatgt taggggaaga aattcttgca
     3901 tatgctggag aatattttaa gggggtatct gctgtaatgt catatagcgg gtatcaaacg
     3961 ggtgtttacg gaacaagaaa tgtttgttct caaataatca ataacgggta tgcttcgttt
     4021 gcatttgttt cagatatgtc gactggttat agtgggaatt taggttttcc tatgcctaga
     4081 caatggtctt ttgatcaatt tgttgaattt accataggaa gtggcaacgg agctgttggt
     4141 atcgatttaa tcgctacatc gggtagagat agcggattta atgagttatc aaatgatact
     4201 aataatgata attacattgc caaatacaat cagaaggtta taaatcagat ggtacgtgcg
     4261 tatcaatatc tatcacaagc tggtcttgat aatccttgga accctcattt gacattttat
     4321 agatatgtaa attattctgg tttgtcatgg gacatcattt cttctcctgt aacagagcat
     4381 gatagaaaaa tttatgatga atataggaat aaattaacta atagcgaggg cctgtataat
     4441 tattttatag atcctaatac tggttcaata ataggtttgc ctcacttaat tgtgacgtta
     4501 caatcacaga tgtttctagc cgacacaatc aatgatagtg tttctgactt tgcaggttgg
     4561 ttaggagatt taatgacttg ctggggtgaa gttaagaaat taggaataca aatggagcaa
     4621 ggtgtatttg cattagtagg tacggctgca ggtggaacat ttagtctaga agatttatac
     4681 caagatgttg acgcggtaaa tgtttatacc ttagcaaaaa gaaattctga ttcaacagct
     4741 ttatcggctt taaaaacgta ctattgtgat aagggtgcta ttgatagatt taatcaattt
     4801 gttaagaaca gatttggttc ttcagataaa atcgcttcag taacaaatag tttattagaa
     4861 aatgacgaaa cagttgatat cttaggtgta gggataattt catttttcat tgataaaata
     4921 gttaataatt cacttgggac agattttact tatcttgatt ttattggaga tgaggggaaa
     4981 gttgcaaatg gattttctga aaaaattatt caatttcaag aaaatagaag taaacttaat
     5041 taagaatcgt ttactattta aacagtgtcc ttttcctttt taaatatata cgataaaata
     5101 agtatatgaa ttgtaaaagg acatgatgat tatggaagaa atagaatgga ataatctgac
     5161 tcaaaataat aaaaataatt ctggatgtgg aattatcttt atgtttgtta tagttttggg
     5221 aatagtagta cttttaataa ctgtcaagat agtagattac tttgcgaata ctcctgaaga
     5281 acaaatacag agaaaacttc ctgagtttga aagagtacca ccttatgatc caagagataa
     5341 ttcatttcaa gtaaaaaaat taaaagagtc tccaaatgaa atgtggtttg aagttacagt
     5401 tgaatcaagc gacttcaaag aaaaaaagaa agaatacaat tatattgtaa aggcaaaagt
     5461 atcaaaaaga tttattttta accctaaatt agaaatatat agtgttgtaa tagaataata
     5521 gtgttaaaaa aattgtaaaa gttaaagaag tcaaattctg atgtgaaaaa taaaatatac
     5581 catggaagac ataaaagaaa aactggataa ctagtttatt taatggcaga ccgttaaggc
     5641 ctgccatttt tatgtatttt agcggaataa atataatcat gcgggagtgt aaacttactg
     5701 tttcaaaaag tatacttttt gacatgcctt gattttcttt gtttatgtat ggcataataa
     5761 ccacataata caatttgaaa cgtttctgat ttaatctgat tgttttgaaa caattttagt
     5821 gaaaaaaggg tagataaaaa cgatgagaaa atttttttat atccgggtca gtagttatga
     5881 tcaatcgatc gaacgacaac tgatttccgc gaaggaatta ggaatccctg aagaatatat
     5941 atttgtagaa aaagccagcg gcaaagattt taaacgccct gaatatcaat taatgaaacg
     6001 gatgtttcga aaaggagatt tactctatct acaatcatta gatagactag gcagaaataa
     6061 acaaatgatt ttagaagaat ggcgagaatt aactaaggaa aagcagattg atatttctgt
     6121 gttagacatg cctctgttag atactgctag ttacaagcaa ctagatgggt tagagacgct
     6181 tatctctgac ttaattctgc aattactcag ctatatggcc gaagacgagc ggaaacgaat
     6241 taatagccgg caaagagaag gcgtagaagc tgcgaaacaa aagggtgtaa aattcggcag
     6301 aaagaaaatt gctttacctc ctgaatttcc tgcgatctat tctgactgga aagcaggaaa
     6361 gataactgca gtctatgcta tggatcaatt gggcatgaaa cgaaatacct tttatcgacg
     6421 ggtgaaagaa tatgaacata ccttgcacca cttttcttga tagatataac agctattaga
     6481 cgggaaggga gagtttgaaa atggacttaa cgaatcaaag ggtcgttttt actggaacgc
     6541 tgcagcaaat gacacgaacc caagccatcg gtttggttca ctctttaaac ggccattgtc
     6601 aatcaagtgt tacttcaaaa acaaatttcc tggtttgtgg tcagtattct aagtcgttgt
     6661 ttgatgattc tctctataca aaaaaggaac aaaccgcgcg ggacttgatt gcgttaggtc
     6721 atgagattac cattttatca gaagtcgaat tttatgcgtt aattagtcaa cagttgaaag
     6781 aatggcaacg gtatttataa tcgttagtaa aatttacaag gaggaaaatt tttatgaagc
     6841 gaaaatctta tgatcgttgg ggcattgaaa taactagttt taatcgaaat attgcttttc
     6901 acgtggagtg tgactgtggg gaattagcaa aaagtgttta tcaaaaaaat tacttttact
     6961 gtcctaaatg ccatcgccga tacggggtac atggaacata gaaacgaaat agtgcggtag
     7021 aaaagcgaat gaatgtagta tatttctaca aatcattcgc tttctttatt ttataaatag
     7081 ttggtcgaga gatcccgttt tcttttgcaa tctctgcaac tggaatgcct tgctttaaca
     7141 tgtccaccac ggttttaaaa actaagcgtt tttgcggatc tgttgagtta atagaatact
     7201 cgattggtcg tcccttatat cggccggctt tttgagccaa taatactcct tgccgctgac
     7261 gttcacgaat tttctcacgc tcgttttgtg caatataact tagcagtgat aaaaacatat
     7321 cgaacatagc ggtatccagg agttcatttc cagtattaaa atctaaaaac tgtgcatcta
     7381 atatttttaa cgctactttt ttctgtttca taaaagcaac tgtatttttt atatcttcat
     7441 aatctcttcc taaacgatct agt
//
LOCUS       DBNICI010000134         7431 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.135, whole genome shotgun sequence.
ACCESSION   DBNICI010000134 DBNICI010000000
VERSION     DBNICI010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.135"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(449..1795)
                     /gene="gor"
                     /locus_tag="AC1ZCC_002262"
     CDS             complement(449..1795)
                     /gene="gor"
                     /locus_tag="AC1ZCC_002262"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HIQ1630490.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(1929..3182)
                     /locus_tag="AC1ZCC_002263"
     CDS             complement(1929..3182)
                     /locus_tag="AC1ZCC_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIQ1630491.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3188..4111)
                     /locus_tag="AC1ZCC_002264"
     CDS             complement(3188..4111)
                     /locus_tag="AC1ZCC_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIQ1630492.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4332..6824)
                     /locus_tag="AC1ZCC_002265"
     CDS             complement(4332..6824)
                     /locus_tag="AC1ZCC_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIQ1630493.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6831..7298)
                     /locus_tag="AC1ZCC_002266"
     CDS             complement(6831..7298)
                     /locus_tag="AC1ZCC_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HIQ1630494.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2041 a         1622 c         1188 g         2580 t
ORIGIN      
        1 atttgttcgg aaaaagtgac taactatgac acaatttttt tcacagtccc ttttttcact
       61 tgatttgatt cgtaccagcg aacttgacga gaactttctt ggtttggtaa ctatatggta
      121 ttcttttaac atcctcgttt gcttcttcta atttatttat acaaagtcac acttttttaa
      181 acatctgttc ttcgatacat ttctatttct tttaaacatc gataccaata ttttgaactt
      241 aagagtgcat accaaatgga ttcatttact cttcgtcagt tacgaagata atcaaaatct
      301 ccaacttccc tacttatttc gttttgagaa ttatcgaagc aatcttacat aaataactaa
      361 aaaacaaaaa accagaaaat cagtatactg attttctggt tttacatggt ttgttcaata
      421 aaaagctgtt ggaactgtta atattaaatt atcgcatcgt tacgaattct tccgctcctg
      481 ttggatggat ggctactgta ttgtcaaagt cttctttagt tgcacccatc ttgatagcca
      541 cggcaaaacc ttgcagcatc tcatcgacac caacaccaat agcatgtaag ccgacgattc
      601 tttcttcttt tccgacacag atcaatttca tttcgcattt ttggcgataa tcatttaatg
      661 caaagtacat tggggtaaag cgtgaacgat aaactttcaa ttcattttcg ccatattttt
      721 ccaatgcttc ttcttctgtc aaaccaattg ttgcaattgg tggatgcgta aagacgactg
      781 tcggaacaag tttatagtca agataagaat ctttctttcc attaaataaa cgttctgata
      841 aacgtctacc agctgcgatt gcaacaggag tcagatctaa tttaccgatc acatcaccaa
      901 cagcaaaaat atgttcatcc gttgtatttt ggtatttatc tactttgata aacccttttt
      961 tgtccaattc cacatttgta ttttccagac ctaatttttc ggtattcggt acacgtccac
     1021 cggcaaaaag tacacaatca ccagttaagg tttcaccatt ttcaaatagc accgtatatt
     1081 cgtcattgtt tttagtaatt tctttagcaa tatgatgtgc gtaagttttt attccagctt
     1141 cttggtacat ttggatcaaa ttgtccgaaa gcattttatc aaacgttcta agtggtcgtt
     1201 cttttcgata tgcccacatg acttcactac ctaaaccacg aaatacacct gaaagttcag
     1261 ccgcaatgta tccagcacct aaaacgattg ctctcttagg taactctgtt aattcaaaaa
     1321 atccatttga atcaattgcg tattctccac caggaatggt cattttactt gcacggcctc
     1381 cagtagcgat caagatttta ggagcacgat aggtttcacc gtttacttcg atcgtttgac
     1441 tatcgataaa agtagcgtaa ccttttatag cctcgacatg attgctatct aatcctcttt
     1501 gataagcacc gtgtagaaaa tcaatatatt tttctcgtct ttctactaga ccggcgaaat
     1561 caacattttt taaatctgct tggataccgt aacttgaggc atctcgttca attgtttcca
     1621 aaatcgtact tgcttgccac ataacttttt taggtacaca accgacgttg acacatgttc
     1681 cgcccaattc attcgcttca atcagcaaaa cttttgcgcc atgcatccct gctctgtttg
     1741 ctgaagcaat accgccagag ccgccaccaa ttacgatgta atcatatatt tgcattttgt
     1801 tcctccttat tgaagagatc aatcaaaacc tttatttaac gatccttttt ctacagaaaa
     1861 caagtgtaac acttttcttg aattttgtat gcgaagatgc ttaaaacagg gatatatcag
     1921 ctttttcctt atttacgttt tcggatcata tctccaggtt tcaccggctg tgcgactggc
     1981 atagttaaaa tcatcattgg atttggtgct cggtcaatgg attcgccttc ttcattatgc
     2041 ataacttcaa cggtttgact gaaatgactg aatcctggac cataaaattc gatttcatct
     2101 cctacagaaa acaagtttct ctgacgtatc gtagcaatct gtgttgcctc atcataagat
     2161 aatacttcac cgacaaattt atatactggg attttacgac gttcaccgaa taattgttcg
     2221 ttttcagttg gtgtattgta gtaaaaacct gtagctaatt ctcgttgtgc tactttccat
     2281 aactcgtcga tccattcttg cttgcagaca tagttttcag gatcttccat ataactgtct
     2341 acagccgctt tatagacatt tgacaccgta gatacataat ggatcgattt cattcttcct
     2401 tcgatcttga agctgtctac gccgttttca atcaattctg gaatatgttc gatcattgac
     2461 atatccactg cactcatcga gaattcttct tccacttcgc ctgtatcagt taaactgttt
     2521 cgttcttgac caaaaggcat atcgaacaat ccatacttcc agcgacaaga ttgagaacat
     2581 ccgccacggt ttgcatcccg catcgacata tggttggaca atgtgcatcg accagaataa
     2641 gagatacaca tggcaccatg gataaatgct tcgatttcgg tatccgtatt tttacgaatc
     2701 tctgccactt cctccattga aacttcacga gctaaaacga cacgttccaa cccttcttct
     2761 ttccagaatt ccaatgtctc aaaattagtt gctgaagctt gagtggataa atggatcggc
     2821 agacctgggg cttctgtcgc acaaatttcg atcaaagctg gatcagaaac aatcactgca
     2881 gaaataccga catcacgcaa actgcggaaa aattcccctg ctccatcttc atttccttca
     2941 tgggtgacca tattagcagc aacgtatacc ttcgcatcat gttcttttgc aaaagcaact
     3001 ccttcagcca tttcttcata agtaaaatta cctgcacggc ttcttaaacc atatgcattc
     3061 ccgccaatat atactgcatc tgctccataa tggatcgcgg tttttaactt ttccagtgtt
     3121 cctgctggcg ctaggatttc tggtcgtttc aattttcttt ttactgtcat ttctttcatc
     3181 atactcctta tttgatagca gaaggatcaa tataaaagaa tcctgtatct aattcacggt
     3241 tttcaggatg caatttactg atttcgctag tcattttttc cgctttttct tgatcccagc
     3301 taccttctaa aatttgtttt tttgcttcgg cgaatagtcg agtgatgttt acgaattttt
     3361 cgcctggagt aaataatccg tccaatttcc atgtacagta atgatgctga taaagttcat
     3421 tcaattcatt cgagagattc agatcattgc tggcaaaaat atgtgtgcca tgactatctt
     3481 cataaataga ataatgtgtc tcttcttttt taggttccga gataaaaaga cctctttcac
     3541 gatctttttg ttcgtcttgt ttcgtatagt tatagtaatt ctgtagcagt ggacgtttgg
     3601 attgatggat gcatgtagca ccatacacaa gggtttctac aggaatatcg agtttttctt
     3661 ccatcgcctt catctcttca aaaggaactt cacgagcaag tactgctcca actgcgcctt
     3721 ttttcgccca gaaattgatt tgacgtgcgc tcgttactaa tgtttcagca tcataaatat
     3781 acggtatacg caattcagga ttttttttca tcgtataaat aatacctgta tcaccaactg
     3841 tgatcacatc tacgtttatt tcttttaaaa aagccaaata ttcaggtaca tttttcattt
     3901 tttcaggatg aatgatccca tttacagcaa tattcgcttt tttaccatac ttgtgtgcta
     3961 attctaccaa ttgtcgctgt tcttctcgtg taaatgaagc aggcagacgc aatccaaatt
     4021 gctcttcacc gaaatatagt gtatctgcac ctgcagcaag caactgttct gcttgttcaa
     4081 tagtttctac tgtggttatg agttcgatca taattttctc ctcctacaac ttcataatag
     4141 tagcatagct ctaaaaactt tccaatactt cttctattta aagtcggatt tttgaaaatt
     4201 atttcacaaa aaatcaaaat aaagttcgat atattctgtc caaatataaa aagagattgt
     4261 gacaaaaatc ttgtcacaac ctcttaatca agtcttacta ttataaaaat tcagctacac
     4321 aggccaagta actataggga acctactgtt tcctttttag gtccgcgaac attcaatgtg
     4381 atcttacctt ttgaagcacc tactgttacg tggtcaccta aacgaatttc tccgccaagc
     4441 aaagcctcgc tcaatcgatc ttcaatttct ttttgaagtg cgcgacggat tggacgtgca
     4501 ccatattcag gatcaaatcc agctttcccg atcacatcga ttgcagatgg tgtgattttc
     4561 acttgaatat cttgttcttt caatcgtttg ataatcgctt tgctcatgat ttggacgatt
     4621 gtatggattt catctttact caatgaatgg aagacgacag tctcatcgat tcgattcaag
     4681 aattcaggtc ggaaagcttt ctttaactct tctaagatac gtttttgcat ggctttgtga
     4741 tctttagtaa catcttggac attgaatccg acattttttt cttctcgaat ttgtgtcgct
     4801 ccgatatttg aagtcataat catgattgta tttctaaagt ccacttttct acctttagaa
     4861 tctgttaaat gaccatcatc caatacttgc agaagtaaat tgaaaacgtc tggatgtgct
     4921 ttctccactt catctaagag aatcactgaa tatggttttt gacgaatttt ttctgtcaac
     4981 tgtccacctt catcataccc tacgtatcct ggaggagacc cgattaatcg gcttgtactg
     5041 tatttttcca taaattctga catatcaaca cggatcaggg catcttcact tccaaacatg
     5101 acttctgaaa gtgctttggc caattctgtt tttccgacac cagttggtcc aaggaacatg
     5161 aatgaaccaa ttggacgatc aggatctttc aatccgcttc gtgcacggcg gatcgcacgt
     5221 gaaactgctt tgacagcctc atcttgacca acgacacgtt catgcagaag tccttccagt
     5281 tctaacaagc gttcgctttc ttttttctcc aactgctgta gagggacacc agtccactcc
     5341 gatacaactg tagcaacatc ttcttctgtt acacgatcag aataaccttg ctcctgtttt
     5401 gcttcaattg cagaaacttt ttgcagcttt ttacgtagtg ctttttcttg tctgcgtaat
     5461 tgagcagcat tttcaaagtc ttgtttttgg atcgctgctt ccttttcttg tactagttca
     5521 tcgatttcta gtttgatcac agttgatttt gttaagtgat cggtttgatc caaacggact
     5581 ttcgctgctg attcatcgat caaatcaatt gctttatcgg gtagttgacg agaagtgata
     5641 taacgaacag aaagattgac tgctgctcgt acagcttcat ctgtgatctc cactccatgg
     5701 tgctcttcat atcttgtacg taatccttga agaatcactt ctgcttcttc aggagttggc
     5761 tcatctactt gaatgcgagc aaaacgtcgc tctaatgcag aatctttttc gatatatttt
     5821 tgatattcat ctagggtcgt tgctccgatc gtttgtaatt ctcctcgagc taacgccggt
     5881 ttcaagatat tcgaagcatc aattgctcct tctgctccac ctgcaccaat taaggtatgc
     5941 aattcatcaa tgaataagat tacttgacca tcattataga tttcgtcgat gacctttttc
     6001 attcgatctt caaattcacc gcgatatttc gtaccagcaa ccaaagatcc catatcaagc
     6061 atcatcaggc gcttttcttg catgtcttct ggcacttcac ctaaaatgat tttttgagct
     6121 aacccttcag caatggctgt tttgcctaca ccaggttcac ccaccagtac agggttattc
     6181 tttgtccgtc ggctaagaat ctggataagc cgtttcactt ctctcgaacg accaacaact
     6241 gggtccagct tgttttcttt tgctaatttc gtcaaatcac gtgctaatga atctagcgta
     6301 ggtgttcctt gcggtgtttg ccgtttatct tgtacattta catttctacg acgattcatt
     6361 ccttttccag cagaatcacc agctagcccc attttttgtt tcagaacttg acgggctttt
     6421 gaaaggctga tacctaagtt gatcatgatt cgagtagcca tgatactttc atcttttaat
     6481 aagctcatca aaatatgttc tgttccaact ttttgagaac cagttttctt cacttcgctg
     6541 ctagctaaag caaaaacttg tttcattctt ggtgaatatg gaagaaagac tcctttaggg
     6601 taagaacgga taccaccata accaatcaga tgttcaattt cttcataaat ttccgtttca
     6661 ttcaaatcca tttcacgtaa tacttttcca gcaattccat tttgctcgac cactaaggct
     6721 agtaaaacat gttctgaact aactgattga tgtttcaacc gttttgcttc ttcttgtgca
     6781 atttctaata ctgcttgggc tctttcagta aaaagttctt ccatctaatc tcacttccca
     6841 tcttcataac ttaaacgttc cagaaatgct cggagaatcc gagcacgtgt atgatcttca
     6901 tattcattga aattcaatgt gttttttgca attgcactca acatcaagtt tccttctttt
     6961 tttgtaatta tttggtcttc ataaagtttt tgaataatag caaaagcatt tttctcactg
     7021 attgtatcat caaataactg atttatctgc tcaagtaatt gttttttatc tgaaatcctt
     7081 acttttgcaa ttcgtatata accaccacca ccacgtttgc tttctactag ataaccacgt
     7141 tggatggtaa aacgggtatt aatcacatag ttgatttgtg aaggaacaca gttaaagagg
     7201 tctgccattt caatccttcg aatctcgatc atttcactat tttccagaat tttctttata
     7261 tatgattcaa ttaaatccga ggtattctga tgtgccatct tattcattcc ctttgactaa
     7321 tattgacctt aattataacg aatttgataa tagaaagaaa gaagaacgct ttttgttaaa
     7381 taaatccttt agaatcaaca aaaaaaaata cggaatatac tattccgtat c
//
LOCUS       DBNICI010000135         7390 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.136, whole genome shotgun sequence.
ACCESSION   DBNICI010000135 DBNICI010000000
VERSION     DBNICI010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.136"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(204..1652)
                     /gene="cls"
                     /locus_tag="AC1ZCC_002267"
     CDS             complement(204..1652)
                     /gene="cls"
                     /locus_tag="AC1ZCC_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HIQ1630495.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1804..2688)
                     /gene="ftsX"
                     /locus_tag="AC1ZCC_002268"
     CDS             complement(1804..2688)
                     /gene="ftsX"
                     /locus_tag="AC1ZCC_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HIQ1630496.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="AC1ZCC_002269"
     CDS             complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="AC1ZCC_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIQ1630497.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3472..4573)
                     /gene="prfB"
                     /locus_tag="AC1ZCC_002270"
     CDS             complement(join(3472..4500,4502..4573))
                     /gene="prfB"
                     /locus_tag="AC1ZCC_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIQ1630498.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4699..7233)
                     /gene="secA"
                     /locus_tag="AC1ZCC_002271"
     CDS             complement(4699..7233)
                     /gene="secA"
                     /locus_tag="AC1ZCC_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIQ1630499.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
BASE COUNT         1975 a         1639 c         1289 g         2487 t
ORIGIN      
        1 gctatactgg ctttgaaatg tgtctctttt aatagcatat attagcttat ttcttgcttt
       61 ccccaaaaaa caagagatac cccaaagtta atttcgctca tgcgagacgc tttctccatt
      121 tctgacgtac tctcagaaat ggccccgtta aaaaaggaca cgagatctcc atctgttgat
      181 ggttctcatg tcctttttat gttttaaagg agcggcgaaa tcaatcttgc taatccttct
      241 ttcatcttga tgattattcc tcggttttca tacatttctt ttgtgagatg cgtagaaatt
      301 ttcgagtctt caaaaaaagc ttttcttaca cgtgaagcca ttcgttcatc ataaaccagt
      361 gtattgacct caaagtccaa gcgaaatgaa cgaacatcga tatttgctga gccaactgaa
      421 acaatgccac catcgataat catcgtcttt gcatgaataa atccattctc atatgtttcg
      481 atccttgctc catactcgat cagctctgca gcgaaagaat acgttgccca gtaaacgaga
      541 agatgatctg gtttgttagg tatttggata tgaactttta cgcctgaaag aagagccaac
      601 ttcagtgctt catgaatcga tccatccggt atgtaataag gcgtttggat caatatctcc
      661 cgtttggcta acgaaatcat tttcaaataa gtcattttga tttgttcatg ttcggtatct
      721 ggtccgcttg tcacgatttg aacagctata tcaccagtag tgtgaatgga cggaaaaaat
      781 ttgggagaat agcctacttc ttttgtatgc tgagaattcc agtccatcaa aaaacggttt
      841 tgcaaggtat acaccgcttc tccgtaaata cgcaaatgat tgtctcgcca ataaccaaat
      901 ttctttacca atcctagata ttcatttcca acattgaacc cacctgtata tccgatttcc
      961 ccatcaataa ctacgatttt acgatgattg cgataattga tccgaggatt aatatacgga
     1021 acaaatagag gaaagaaaaa tgcgacttct ccgcctgctt ttttcaaatt ctgaaaaaag
     1081 ctggaagaaa cttgtgtgga accccatgca tcaagaagga ctctgacttt tacccctcgt
     1141 cttgccgcct ctgtcaatgc atctcgaact tcttcaccta atgcatcact gcgataaata
     1201 taatactgga agtggatatg gtcttctgcc tgcttgatat cttcaatcag cgcatcgaat
     1261 ttttcacgac catctgtaaa taaagtgatt tcatttttag tcgtatagag cgtactttca
     1321 aataccgtga gcatatagat caattgtttc acatctactt gtccagtagg cggatgagga
     1381 taaagccctc gcatcaatgc ctgtttgtct tcttcaattt ccaggttctt accgattttc
     1441 ccttggatct tcaaatcaaa gattttctct ttggaaatcc ctcttcctaa aaaaatatat
     1501 aaaataaatc ctacaactgg aatgaacatc aaaacgagca gccatgccca tgtttgagca
     1561 gtttcctttc tttctcgaaa aacgatgata aaggaaagca aaatattcat aatcaaaagg
     1621 atcgtaaaaa aattgtcaac gataattttc acggcttcac ctcttcttct accaacttaa
     1681 gaatacccaa gttgtttttt aaagtaaagg gaaaaaagcc gcttccttct attctcctag
     1741 ccaataaaaa aagaaagctg gaacgaaact gtccaacttt ctcattttat ttatattgtg
     1801 gtattagaat ttcaagaaac gtctcataga aatgatcgag ccaagtgaac caatgatcac
     1861 accaatgccg accattaaaa gattgatttt ccaaacgata ttttgtggat gaatcaatga
     1921 ataatgggaa cgtaataatg atggattgat caattggaaa aaccatgcat aaccaaatgt
     1981 gatgatcaaa attggaagaa ttgcaccgat cagaccgatc cagccacctt caaggaaaaa
     2041 cggccagcga atatatccat tttttgcacc gactaaacgc atgatttgaa tttcacgctg
     2101 tctagacaaa atcgtgatcc gaatcgtgtt cgaaatgaga aacatcgcta cgaacagcag
     2161 caatgccgct gcaactaatc cccatgtccg aactgcacca gcgatttgga aaatccgatc
     2221 tgaggaagct ccaccgtaat cagaacgaga aacattcttc aaatctgctg cttcttctgc
     2281 tacttgtttt gtataagaag gatcagtagc acttactaca tacacatcat ataacgggtt
     2341 attgtcacct tcaaacaatt tccaggaatc acccatttcg ttttggattt ttttcaattc
     2401 ctgttctttg cttgaaaatt caactttttt aacatgatct aagtcattca attctttttc
     2461 tagctttttc atctcttcgt cttttgtacc gatatctacg aatacagata catcaacgtt
     2521 tccttctata tcctgagcta atttagttgc attcatgata actgccatga atacaccgac
     2581 taaagtcaac gtgatcgtta ccgcactgac tgaagcaact gtcatccagc cgttacgttt
     2641 caaactcttg atactttcta ataaatggcg gaaaaaggtt ctaatcatcg tatccatatt
     2701 ccccttccgc ttgatctcga ataatgcggc cgttttcgat agcaattact cggtgccgaa
     2761 tcgtatttac gatcgtactg ttatgtgtag ccatcacgac agtagttcct tgggcattga
     2821 tccgatctaa taatttcatg atttcccatg agttttctgg atccagattt cctgtcggtt
     2881 catccgcgat aagtacttta ggcgtattca cgattgcccg agcaatagag acacgttgct
     2941 gttccccacc ggataattcg ctaggaaaga ctcgaacttt gtgtttcaaa ccaacaaggt
     3001 ccagcacttc cattactcgt tttttgatat ctcgaggttt tctgccgata acttgcatag
     3061 cgtacgcaac attttcatat actgtttttc ttggtaataa tttatagtct tggaacacca
     3121 cgccgatttc tctgcgtaga taaggaactt cgcggttttt gattgctatt agatcatgac
     3181 ctgcgacgtt caaagttcct tttgaagctt tttcttctcg atacatcaat ttgatgaatg
     3241 tagatttacc ggctccagac ggaccaacca catagacaaa ctctccttgt tcgatgacaa
     3301 tcgagagatt gcggatagcg gtcgtaccat ttgagtactt cttcattaca tccttcattt
     3361 caatcatggc gttctctcca tatctttttc agtctttagc tattatagca tggcaatgtt
     3421 tcaacatgct tgcaaaatct tacaatttta tttcaattta acaatgggtt cttattgtag
     3481 atgcattttt aaatagccgt caataaataa atcaaggtca ccgtccataa ctgcttggac
     3541 atttcctgtc tctacgtttg tccgatggtc ttttaccata gaatagggat ggaaaacata
     3601 agaacggatc tgtgaacccc agccgatttc tttttgttct cctcgaagtg cagctgcttc
     3661 ttgttctttc ttttccactt ctaattgata aagcttagct tttaacatcc ccatcgcttg
     3721 ctcacggttt ttcaattgtg agcgttgtgc ctgactagct actactgttc ctgtaggcaa
     3781 gtgagtgatc cgtacagcag attctgtttt attgatgtgc tgaccacctg caccacttgc
     3841 tcggtacgta tcgattttca aatcatctgg attgatttct atatcgatcg tttcatcaag
     3901 ctctggcatc acatctaccg aacaaaaaga agtatgacgt cgtttcgctg agtcaaaagg
     3961 cgaaatccgt acaaggcgat gaacaccttt ttctgatttt agataaccaa aagcgttatg
     4021 tcctttaatc agcagggtca cactcttgat ccctgcttca tcacctgctt gataatctag
     4081 tgtttctact tgatagccat gctgctctgc ccaacgtgta tacatacgta aaagcatgct
     4141 gccccagtct tgggactccg ttccgccagc ccctggatgc aattcgacga tcgcattgtt
     4201 tcgatcataa ggtccgtcta acagcataga taattcatac gtcttcattt tttcatctaa
     4261 tgcattgata cgtgtttctg cttcttcttg cattgctgca tcaggttctt cttgcagcat
     4321 ctccaccatc aattcgattt cttctagttc ttcggcaaga gaatggaatt ggtcataggt
     4381 ttctttatta gcgttatttt cgctgatcac tttttgtgcg gcttcgctgt catcccaaaa
     4441 accaggttca gccatttttg cttctgcttc cgcgatgtct tcttccagct gatctaagtc
     4501 aaagagacct cctgaaactt gtgacctttt cctgcatttc tgtcaggaga tttcgaattt
     4561 cggatatttc catgtttttt ccttctttca ctatactttt ttcattatat ctagtctaac
     4621 agaatcagca aataacagaa agaaggtgag acgaattctt aaaaaaagaa tttgtctcac
     4681 caatcctgct gtcagaaatt agtttgcatt ttttccatga cagttcttat attttttccc
     4741 gcttccacaa ggacaaggat catttcttcc gaccttattc acatggattg gctgtttttt
     4801 cgcgctagta ttactatttt ctttggcatc tccgtcttct gagtgagaag ctgttccttg
     4861 agaaacctgt tcacgctgta cgttttgacg gatctcagct ttcataaaga ttcgagtgac
     4921 ttcatactcg atggcgccta ccatatcttc aaacatctta tagccttctg tttgatattc
     4981 gactaatgga ttgttctgtc cataagcacg taagccgatt gactgacgta attgatccat
     5041 tgcatcaata tggtctgtcc atttagagtc cacaacacgc aagatcacga ctttttcaaa
     5101 ttccaacagt tgctctggac catttagttg agccgctttg gtattaaata cttcttgtgc
     5161 tcgttcgttt agatactctt tgatctcctg agctgttttt ccttcgatat ctgcaagagt
     5221 aatgctatct tcatggacaa gagtactgcc ggcaaaatcg atgatacttt ccaaattcca
     5281 attttctttc tctaattgtg tatgcgcatc tactacacga ctgattgtcc gtttgaccat
     5341 gttcattagt ggcttggata agcttttttc ttccatgatt acttcttgtc gctgtttata
     5401 gattacttca cgttgttcac gcatcacatc atcgtattgt aagacgtttt tacgtgtgtc
     5461 atagttattt ccttctacac gtttttgtgc agattctact tgtttcgtca gcatcttgct
     5521 ttggataaca gcatcttcct caccgatctt catccgatct aagaaagctt tgattcgttc
     5581 tgaaccgaat cgtttcatca aatcatcttc caatgacaag tagaactgtg acataccagg
     5641 gtctccttga cgtcctgcac gtcctcgaag ctggttatcg atacgacgag attcatgtcg
     5701 ttctgtcccg ataactgcta gaccaccaag ttctcttacc ccaagtccta acttgatatc
     5761 tgttccccga ccagccatgt tggtagcaat cgtcacagca cctttttgac cagcattcat
     5821 gatgatctct gcttctttaa agtgattttt cgcattcagc acttcatgag gaatacgttc
     5881 ttgattcagc atatctgata ataactcaga tgtttcaacc gcaacagttc cgactaacac
     5941 aggttgacct ttgcggtagc gatctttgat atcttgcacg acagcgtgga acttgctttg
     6001 taatgttggg taaagcaaat ctggacgatc atcacggata accggacgat ttgtcggaat
     6061 ctggtatact tggatgttat aaatttctct gaattcttct tcttctgttt tagctgtacc
     6121 agtcattccc gcaagtttct tgtacatacg gaaatagttc tggaaagtga tcgtcgccat
     6181 ggttttcgtt tcgtcttcga tctctacgcc ttctttagct tcgattgctt ggtgaagtcc
     6241 atctgaataa cggcgtccat ccatgatccg accagtgaac tggtcaacaa tcagtacttt
     6301 tccgtcttgg acaacataat cgatatctaa aagcatgata tagttggctc tcaatgcttg
     6361 atccaagtga tgggtcaatg cagtattttc aatgtcgtat aagttatcca aaccgaatgt
     6421 ttcttctgct ttttcgatcc ctgcttcggt taaactgatt gtttttgatt gtacatcgat
     6481 tttataatct tcttcttctt tcaaacgttt cacgaagttg tctgctcttg tatataaagc
     6541 tgtggatttt tctgcttgcc ctgaaatgat cagcggcgtt ctcgcttcat caatcaagat
     6601 tgagtcaact tcatcgacaa tggcataatt cagcggacgt tgtaccattt gatggcggta
     6661 aacgaccata ttgtctctca agtagtcaaa tcctaattca ttattcgtac tataagtgat
     6721 atcacaatta taagcctcgc gtttttcatc cgaagatttt gagttgatat tcaaccccac
     6781 tgttaatcct aagaaattat aaagttcgcc catttccgtc gagtcacggg ttgctaaata
     6841 ttcattgacg gtaactacgt gtaccccttc gccagtcaga gcatttagat atactggcat
     6901 cgttgcggtc aaggttttac cttcaccagt acgcatttca gggatatttc catcgtgaag
     6961 cacgattcca cccatcaatt gtacgtggta tggatataaa cctaatacac gttttgctgc
     7021 ttctcggacg actgcaaaag cttccggaag taattgatct aatgtttctc ctttttggta
     7081 tctgcccttg aattcagcag tttttgcctg taattgttca tcagttaatg ctgccatttg
     7141 gtcagcgtat gcttcaactt tttttgcaat gctatctaaa cgttttagtt ctttcttatc
     7201 gttttcgata agtgaacgaa tcaaattggc catgcgttgt gtaatctcct tttccttctg
     7261 tgtattatcc agactatgta gactttactt ttcattttat cattagttat agataattga
     7321 aatatcttaa gcgtattaaa gtaagaactt cataatttat tcatatttca tctaaaagag
     7381 gaggaaactc
//
LOCUS       DBNICI010000136         7383 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.137, whole genome shotgun sequence.
ACCESSION   DBNICI010000136 DBNICI010000000
VERSION     DBNICI010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.137"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(90..386)
                     /locus_tag="AC1ZCC_002272"
     CDS             complement(90..386)
                     /locus_tag="AC1ZCC_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HIQ1630500.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            741..1097
                     /locus_tag="AC1ZCC_002273"
     CDS             741..1097
                     /locus_tag="AC1ZCC_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HIQ1630501.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1107..2006
                     /locus_tag="AC1ZCC_002274"
     CDS             1107..2006
                     /locus_tag="AC1ZCC_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIQ1630502.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            2526..3254
                     /locus_tag="AC1ZCC_002275"
     CDS             2526..3254
                     /locus_tag="AC1ZCC_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HIQ1630503.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            3399..5426
                     /locus_tag="AC1ZCC_002276"
     CDS             3399..5426
                     /locus_tag="AC1ZCC_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin-like domain-containing protein"
                     /protein_id="HIQ1630504.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            5534..6631
                     /locus_tag="AC1ZCC_002277"
     CDS             5534..6631
                     /locus_tag="AC1ZCC_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HIQ1630505.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            6938..>7383
                     /locus_tag="AC1ZCC_002278"
     CDS             6938..>7383
                     /locus_tag="AC1ZCC_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1630506.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT         2565 a         1190 c         1466 g         2162 t
ORIGIN      
        1 caaatcgcag ttgtcacaat ctcttctaaa caatgttcaa tcagctgacc ttcggtatta
       61 gctcaaagca ctgatttcac aacctctatt taggcatgtg tctcagcaag cggttgatag
      121 ttgatgatct gcatttttcg ataaaaatcg gcgggaagct tactccaccg ttttagtaat
      181 tgatagaaat aactctcttc tgctttgatt ttatactcgc ttaatatcaa tccaaaacta
      241 ttcgtttttt ttgaagctaa ttttttataa atatccaatg ctttggtttt atcttcattg
      301 acaaagagtg tttttacttc aatataagaa ccctcgatat tttgtgtttc ttcgataaaa
      361 tagatgtttt ttatatattc cttcatagtg attcctccaa agaagtcttt tatttgataa
      421 gtttactcta ccgaagagtt gttaataatc cgtgaacaaa aagtaattct ttttccaata
      481 gttgttaaag gttttttatt ttttatagta ttttttgtta tttaagaaag gaataaggtt
      541 aaacaaataa caggttgctt tttgggtctt tttccgttca agaaactgaa cgaagaaaag
      601 ggaaagttgt ctcttttgtg ataagagaaa tcataataaa cgaatgactt actttccaat
      661 aaattgagaa cagtcagaac cagacaaatt agttgcatct gatgaataaa gtgattattc
      721 tatgacatga ggtgatacag atgaaattag aagtgacaaa agaagcacaa gaagtattga
      781 agaataaatt agaagcagac gatcaacttt tattagatat cgaaaacggt gacggtccat
      841 ttgcagaaag ccaagtcagt tgccaattgg atacggcatt tcgattgatc attgtcaaaa
      901 aagatacaca aacagatcta agtctttatt ctgtagtggc tgatacacca gttggaccaa
      961 tcaaaatcaa agacagtgca atactttaca tggatgatcc atccacactt gctttagaac
     1021 caacttataa tagtttacaa ttaaaaggac caagcggctt gagaaaaggc aacttgcaag
     1081 ttgttcgaaa agactagagg gataaaatgg gaaaattggc gatcatcagc gacctacatg
     1141 tggacatcaa taaattcggc gaatcagagc tgatgcagct ttggcaagtt ttgcaaaagc
     1201 acaagatcac tcggcttcat ctagccggag atacggctaa taaagtagac cgttgcctag
     1261 ctgttgtcga atttttcgtc aataaaggat tatctacgac atttcatttc ggcaatcacg
     1321 agttggcaga tgtgcccaat gaacagatga tggaccattt tccagaccca cattttttaa
     1381 atgaacggta tattgcttta aatgagcaga ccgttctttt agggatgaat ggctggtacg
     1441 actatcagtt ttccgaattg aaagattacg aggcaatcgt acgaatgaag cgtttgtatt
     1501 ggtatgatcg gttcatccag cgtgggaaaa cagatctcga ggtcaatgca gaaatgcttg
     1561 cagctgcaaa aaatttgcta gaccagctgg aagcaaaaaa acttgaggtg attttatcca
     1621 ctcatttcgt tcctaaacgg gcatttatca tgtatcaatc tgctccttat gagcgttgga
     1681 acaaattaaa tgcattttta ggatcagaat cattcggaga attacttgac cgttattcaa
     1741 atatcaaaca agtagttttt ggccatacgc atcgtcgctt tgaagataaa tccatcggtc
     1801 acacgattta cagctgcaga ccttttggtt attattatga atggcagctg acccgagatt
     1861 ttgtcttgaa agaacagttg ctagacagct atgatcctat gaaattacgt gtcctcttgc
     1921 gtgcccatca cccagctttt caggcctatc aaacacaaca tttgcaagag gaatttgaac
     1981 aagcgatgac aatcgttccg tattgagaca gcattagtaa tgtgtaaaag aaaatacctt
     2041 gtaaaacata aggataagta agagcagtag atccatttat cgtggatatg ctgctttttt
     2101 tgcggaaaat aatcagcgaa tgtttatttg atcggatgat ttgtaactcg cagagtacta
     2161 gatgcaagcg tgtattcttt ttttgaacga taatcacaaa aaaaaatata atatacattt
     2221 tttatttaat tataattaat attgattgaa gtctaggcag tcaagtcaca ttttgataga
     2281 taggaggggg acttacgaat tattttaaag gctatgacag ctgtatttct agattgtgtt
     2341 tgcatcaagt tgcaataaaa actagaaatc aaatcgggct gataatcatt tataactaat
     2401 cgatacatag gaccaaatat aggtctatgt tcttttattt cataagtatc gtaaaacaga
     2461 attttgctat ggattaatcc aatcgactga tatgtttagt aaataaagaa agaggaatgt
     2521 caatgatgaa aaaagaaatc gtattaacag cagcaatgat gttattcagc atggtagcaa
     2581 gtacgacttt tgtttctgca actgaagtat acccaaagga atataacaca gaaggaacga
     2641 ttacatttga agcaggagat gagggtgtaa ctcctcctgt tgatccagag aatcctgatc
     2701 ccaataaacc tgtcgatcca tcagatcctc caagccctgg aacaggaggg gcattatcga
     2761 ttgattatgg atcaaaattc aaatttggga ctcaaaaaat ttctactgcg gataaaacgt
     2821 attatgcagc tgctgatgtg atgaacgatg ggtctagaaa accgacatat gtccaagtga
     2881 cagataggag aagtacctta tctgggtgga aattaagtgt ctctcaacca gaacagttta
     2941 aaactgccag tggggatgaa ctagttggag cacagctgaa atttacgaaa gggcaggctg
     3001 tttctttggt tgatccaaca tatacgccgc aaacggtgaa ttctgaatta acgttgacac
     3061 cgggaggaaa caatacgtta gcaattaatg ctaaatcagg aacaggtgtg gggacttggg
     3121 tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc ggtacagctt tcggtacccg
     3181 gtaaatctgt caagttagcg caacaatatt ccactaaatt agtttggaca ttagaagata
     3241 cacctaacaa ctagtttact gaaaagaact gttaaaaata aatacgtagt catattgtct
     3301 tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa atttgtttgg atacgaatag
     3361 ttcatctagg aatttaacga tcaggaggaa aagatatatt gaaaaagagg aggatcgtgc
     3421 tcattttaag cagtctctta gtgactaata tctttccgat catagggtac tctttggaat
     3481 tgaatcagtt ggagcatgga aatagaagag acgaagaaag caaactcact tcgacattcg
     3541 acagtttaat agaatccaac cagacacaag aaagcagtga ggatttaagt atttcgagta
     3601 gtggagagac cactgacaac gtagaagctg agggttcgga caaacaagga ctagaagatg
     3661 caccaatttc tgcggttgag ctgcctgacg atcccaatat tatttctatc gataaaattt
     3721 ttcaaacacc tatcggtact agcacgtcca tattggaaaa tgggaagtta ctgcagttga
     3781 acccagcaga aaggtctcaa agaggagcta tttggtcaaa taagaagatt agtttactct
     3841 cggattttac atttaaatct tatttatact taggaaatca acgagataga gcgggagatg
     3901 gcatgacctt tacattgact ggcgatccta gaatggctag taatcccagt caagttattg
     3961 gatcgccagg tatgggaatt ggctcatatt ctactcgttc tggccaacct tacattgcga
     4021 atgcgctctc gattgagttt gatacgtata aaaatagtgg ttcttctgat cgaatggatc
     4081 gagagataac tgctgataat agagggcatg gacatgtcgc ttttgtcacg cctaaaccaa
     4141 ataataataa ttattctgga gagcatactg gcgtgacggt agctcctaca tttctttcta
     4201 atggcacatg gcgcttgtta ataattaaat gggacgcgga gcaaagaagt cttacttatg
     4261 atttggaagg tgtcggtcgc tcttctcgga ttatacctga tctcattagg cagtttggtt
     4321 ctacagaagt aaattggggg tttacttctt caacaggtac tttttttcaa gaaaatgctg
     4381 tcgctgtcac gcgacttcca tcgaatgtca catcaacagc tgatgtaaaa gtcaacgata
     4441 gtgattatgg ggccatcgct gaagctggag gaaacgataa gataactatc agaaatacat
     4501 tgaaggtcga gggggatttt cttgaatata agaaaatgca gactagtatc gacctcccac
     4561 aagaattaac tattcccgag catatcttga tcaatggaaa tcaaattgat gccaaagata
     4621 tcttgataac cggaaatcga gtcacgctaa atctgacaga ttattttatt gctggagata
     4681 cgttaataat cgaattggag acaactttgc aggaagtaat tcctgaaaaa gtactcacta
     4741 tgcattttga ataccttgaa aacgggaatt tgattgagca atcgaatgaa ataaacctaa
     4801 agatcgcaaa gctgaaggag atagatatcc atgtttatta taaagataaa gacagtcaga
     4861 ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg agagaagtat aaagaaactc
     4921 ctattgatat tgaagggtat gtttttgact cggattcagc gaatacagaa ggcatcttct
     4981 ccgaaaatac agaggatatc tatttttatt acagaaaagg caaactttat ctctcaactg
     5041 ctcctcaata tgtcaatttt ggaaaacata aaatatccag tagaccatta agtgaattcg
     5101 gacagttttc aggtgggttg aaaattatag atgaacgggc ttttggaagt tggaaattac
     5161 aattgaaaca agagaaagtt cttacaaatg gagattttga gcttccagaa gctctttcct
     5221 ttgttacctc tgaaaataca acggttatag ggacatccgc tgttacgatt ttcgaaagtg
     5281 atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc tacagctcat cgtggaatca
     5341 gaataaatat tcctgtagaa aaccaaagaa tcggaacctt tgaggggaaa ctatcttgga
     5401 tacttgcaga tgtcccagga aattaacagg aagagaagaa tataacaaaa ttattgagtt
     5461 tcaagaaggg cacaggtacc ataggctctt gtgtcccaca gagacaagat tttgttatag
     5521 tcttctctta catatgatta aaggagcgat tttcttgaga ccaaaacaac aattatttat
     5581 aacactccta gttattttag gggcactttt taacaatata tcagtgtcag ctagtgcatt
     5641 taatttttca gtgacgccga taacttcaga gaatcaaatc gacaagagaa aaacttactt
     5701 cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa gcggaattga gaaatgatac
     5761 agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca accaccaatt caaatgtgat
     5821 ggttgaatac ggaaaaaacg agattgaaaa agacatgagc ctgatctttg atctggtaga
     5881 ttatgtgagc tatccacaga tagtgacgct aaaacctaaa agtgtccaaa ccgttgcttt
     5941 tcatgtcagg atgccgaatg agcgatttga tggtgtattg gctggtggga tcacattcca
     6001 agaaatccag acggaaaagg accaaacaga aactaaggat caaagcttat cgattgagaa
     6061 tgaatacacc tatattattg ctttgttgat gcaacagaca ctgaacgaag ttgctccgaa
     6121 ccttactctt catgaagtta aaccagatca aatcaatgca agaaatgtca tcttagcaaa
     6181 cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt attgaaacaa aaattacaaa
     6241 aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa ggattgcaga ttgcgccgaa
     6301 tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg ctaaccccag gtgaatatca
     6361 ccttacaatg actgttttag gaaatgaaaa tgaaaatgga aaattttcga gaaaaaaagg
     6421 aaatactacg atcaactata ccaatcaatg ggtatttgaa aaagatttta caatagacgg
     6481 aaaagtagcc aaggaattga acacgacgga tgttacgatt aaaaaagaca ataattggat
     6541 ttatatctta gtagcttcac ttttcttatt gtttatttta ttatgcatac ttttgtttgt
     6601 tttgagaaaa aaagaagaga aagaaaagta aggttaagat aacaaatagt aaatttttat
     6661 tattgtagga tggttgggct ctttttataa caatataaaa tataccattt tattaataaa
     6721 aatgcatact agtaaatgat ataattctaa taacaggttt gatcgcgaga aaggccaaat
     6781 aaaacgctct ccaaaatgtt aaaaaaaaga caggacgatg tatattatac aaacaagaat
     6841 cgctgacaac agtgactcgg atccaggaaa tcagttttat ccccagctag ttggtgaatg
     6901 catggaaagt aatatacgta gaaagagctg atgactaatg aattatgagt tttttttaag
     6961 caaagaagat cacagaagtt attcattact taaatattta gaagcaagct caaatttatc
     7021 agagtcaata tcgaatgttc aagaagagtt gtcgctatcg acatttctct tgaaaaaaac
     7081 gatcgataag ctgaaacatg atttacaaaa gttcgatcta gagaaaaatt tgcgagtaac
     7141 cgtcagtgaa ttggatatat gtctggaaat taatggaaaa tgttctagca aagctctgtt
     7201 atctcgttat ataacggatt ctttatctat gaaaatggta ttggctttct ttcaggaaaa
     7261 atatatctca gtagaaaatt ttgctaaaca gcatcacgtg agttattctg tggcttataa
     7321 agtattacaa agactaaaaa gaaatctaaa aaaatatcaa attttttttg aaaagagaaa
     7381 gtt
//
LOCUS       DBNICI010000137         7352 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.138, whole genome shotgun sequence.
ACCESSION   DBNICI010000137 DBNICI010000000
VERSION     DBNICI010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.138"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(238..2196)
                     /gene="nagE"
                     /locus_tag="AC1ZCC_002279"
     CDS             complement(238..2196)
                     /gene="nagE"
                     /locus_tag="AC1ZCC_002279"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HIQ1630507.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2193..3065)
                     /locus_tag="AC1ZCC_002280"
     CDS             complement(2193..3065)
                     /locus_tag="AC1ZCC_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HIQ1630508.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3407..4279)
                     /locus_tag="AC1ZCC_002281"
     CDS             complement(3407..4279)
                     /locus_tag="AC1ZCC_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIQ1630509.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4407..4976
                     /locus_tag="AC1ZCC_002282"
     CDS             4407..4976
                     /locus_tag="AC1ZCC_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HIQ1630510.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5043..5780
                     /locus_tag="AC1ZCC_002283"
     CDS             5043..5780
                     /locus_tag="AC1ZCC_002283"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIQ1630511.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            6011..7279
                     /locus_tag="AC1ZCC_002284"
     CDS             6011..7279
                     /locus_tag="AC1ZCC_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HIQ1630512.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
BASE COUNT         2303 a         1449 c         1468 g         2132 t
ORIGIN      
        1 ataccgataa actggttttt gaacaccgtt tatcagaggt tgtaaaaaag ctgttctgca
       61 tcaagtaata agaagagtca aataaaaaat agctcttcat atgttacgtt tggctggact
      121 tattaccgcg gagttggcag gagaacgccg tttttattcg gaaaaaggag ctgagacaag
      181 atttttgtct cagctcctgt ttgtttatcg ataaaaaagt ctggggaaga cgatggctca
      241 ggctttgttg gaaacagagc cgattttatc atttgctttt acatcacgtg cgggttcgat
      301 ttctaagctt tggactttgt cgccatttgt gaatacaacg accatatccg tgtttttgcc
      361 tgcagattga atagcggcaa gatcaactaa agcaatcaat tgacctcttg cgactttttg
      421 accttctttt acatataaag tgaatggttc gccttttaag tcaacagtgt tgatgcccat
      481 atgaagcaag acttcgatac cagcatctgt ttggattccg acagcatgtt tcgttgggaa
      541 aatgtttgtg atcgtaccag cgattggaga gaagatttct ccattttctg gtaatacagc
      601 atagccgtca cccatcattt tttccgcgaa tacatcatct gaaacttcag tgattggaat
      661 cagtttccca tttgcgatag cgtaaatatc ttgttcgatc gttccaccgg caaatgtttc
      721 tgttttaacc acggtgtttg tttgtgcagg tcgtgttgca gcaccaccat gtagttttgc
      781 catctcatct gcaacgaatt gtacttctgt acctacgatg acttgcgcat tttttccgtc
      841 gatgatcttc ataccaggta cgccagtggc tttgatttta ttttgatcga tcaggcctgt
      901 atctttgact tgcaaacgaa ggcgtgtagt acagttatcg atcgaagtta cattgtcggc
      961 tccgcctaat gcagcgtaga ttttgcttgc ttgtgccgca tatttattgt ctgaattagt
     1021 cgttgcagta tcgtcatctg aaagatctgc ttcctcgcgg ccaggagtca tcagatggaa
     1081 ttttttgata gcaaagttaa agccaaagta gtaaatcact gctgttacta atccttggac
     1141 gatcaacatc aatggctgat tcgcaatcgg atttttcaaa gataggaagt aatcgacaaa
     1201 tccggcacta aaggcaaatc ctgctgtcca atggaagaat gcactcactg ctaaagaaat
     1261 cccagtgaaa attgcatgca agacatataa tggccaagca acgaacataa atgagaattc
     1321 aagtggttct gttacaccag taaagaatga agcaaaagct gctgctaaca tcaaggaagc
     1381 tgtttttgct ttgttttctg gacgtgcatt tctgtaaata gcatatgctc ctgctggaag
     1441 cccgaacatc atgattggga aaaatccagc ttgatacatt cctgttacgc cttttgttcc
     1501 ttcagaagcc aaaaatttac caatatcatt gattcctgct acgtcaaacc agaacacgga
     1561 gttcattgct tgatgcaatc ctgtagggat cagtaaacgg ttgaagaagc cataaagacc
     1621 tgcaccaccc cagcctaaac tcaaaatgaa tttagcaaaa gaaactaaag cagagtaaac
     1681 cggaggccat acgagcagta gcactgcact gatcagcaac atgacaaagg cagccaagat
     1741 tggcactaga cgcttgccgc taaagaaaga taaagccatc ggtaattttg cttcgtggaa
     1801 acgattataa agagctgccg caatcaatcc ggcaataata ccaataaata cgttactgat
     1861 ctgagcaaaa gatggatcaa ctgattctgg tttgatattc aataaagtag ctactgaatc
     1921 agtttttaaa acatttactg gaacttcaaa tgcgaccaaa cctgccaagg cagcagcacc
     1981 tgatttgtcc actgacatcc ctagtgctaa cccaacggca aacagcaaag gtaattgacc
     2041 taaaactgcg ttaccgccag cttgtaaaaa atgagcgaca atgtcgttac tataagatgc
     2101 ccaccagttt ccgatcccta ctagtaaagc agcagcaggt aagaccgcaa ctggaagttg
     2161 aagggaacgt cccattcgtt gtagatatgc tttcaatttt tttcctcctc atgattgtta
     2221 acatttgttt gtacgattgc gcttcttaaa cgctcgatat ggatggccaa gtaacccagt
     2281 tcttctttcg tgatcgcgag ctggtattct ttggtcagca acacacggat ttttttagaa
     2341 atcttatagc tttctgggta ttttcgtttg accatttcta cgatttccgt gtctaaaaca
     2401 gcatattgtt gcgcatgggt tcgttgtaga agcagtcgta aatgattcac cagtcttgaa
     2461 taattcagtg aaaatggttc tgaatggata tctgtttcca attctgattc cactaaatgg
     2521 atgatgtcag atacaatcgt cacttcacgg atactgcgat ggttattgtt ccgaccgctt
     2581 cgtccgctgt gaatatggat tgcgatatat cctgcttcgt cataagtata tggaatccca
     2641 agctcttttg tcagatactc tactgcccat tgagcaatac tgaattcatc actgtaaagc
     2701 acttcgattt ctcttaaaag tttgttgcgg atgacgatac cgttattaat gttctccgca
     2761 gaaaaagcaa tgtggtcggt caaagcgatc aatacatgtt cattcaactt ttccatcaaa
     2821 acagtttccg catgatcaat gatcgtttct gctgcaaata aaaagcgttc atcaatctga
     2881 ttaagtaaat tttgcaactt gatttgacct tctggttcca tgacaaatat ccgttcgaca
     2941 tcacgatcaa aaatcaaatc atttcttttt ctgtcaaatc caatcccttt tccaatagct
     3001 actttttcct gtccaccgtc atctaccaat actgcatttt gattaaagat ctttttgatt
     3061 ttcatgcgcg cacccttttt gaagatttgg ttttcacgtt tgctagacca gttgattgcg
     3121 gccaaacaaa aatagacacg aaaaacctcc tagggaaacc atgttcctca gagatttttc
     3181 atgcctagta tgatctagtc acatgaaacg aattattgtt aacgtaatca attttatata
     3241 gacagttatg ttttgtcaat tcttttttta taattatagt aatttactgg aattatcaga
     3301 aataaaagga aggaacaaat taagaaactt atgttattta atcattggat cttttgctta
     3361 acttattcgt tgttaaaaag ctgaaagcga aggaaaaaaa ctctacttaa ctgtatttct
     3421 ccgttaggac atctaaaaat ttacgagcag caggagaaag aggctggtct tttttccaaa
     3481 tgagattcag tgaagcttgt aatggcggat cgagtgggat gaatactttt tccgtcccat
     3541 aagtattgat gatcccatct agagctaatg caccaaaagt cccgttatcg accatcaaag
     3601 aagcattgta caataagtta tatgttccga cgatcgataa ctgatctatg ctgaaaccta
     3661 gccattcagc tagttgactg tccactgacg tttgccggga aatgatcaaa ggaacagttt
     3721 tcagttcctg cggatggatg actgtttgct ttgctaaagg atgattccga tgtatcaaaa
     3781 gtccccaacg atcttgggct ggcagtcgaa atgactggta tttttgtttg tttgtcggtt
     3841 cgattacgag tccaaaatca agtaatccgc tatccagttt gtctatgata tcatctgcat
     3901 tcccgctata taaatgaaac tggatatcag gatgcgcctt gatcaacgaa tgaaggctat
     3961 cagaaatgaa agagattgct ttagtttcgc ctccgccaat gtagatttct ccagagatga
     4021 ttgcgtcatt tgttaaattg ttcgccgttt tttctaccaa cgacaagatt tcttttcctt
     4081 ttgaataaag atatcgacca gaatccgtca actgtatctc tcgggagcct cgagtgaaaa
     4141 gtgtcacacc aagttcttct tctaaatctt tcagttgttt tgagagagta ggttgcgtga
     4201 tatgcagaga ttttgctgct cggctgatat ttttttctct agctactgct aaaaaatacg
     4261 tcagtatacg aatatccatt ttttccctcc tgtttttaaa aaagcatacc actaagtata
     4321 gttaaaagga atagttaagt atgttttata agtattagac atcataagcc gtccgttata
     4381 aagtaaggat aaaaggaggg agcaaaatga caagcctcat cgaaaaacaa atcgtcaata
     4441 aagagattcg agtaacggat ccgctctttg aagtgatcca tcagatccaa gcggaaaacg
     4501 aagagccttt agcagcgctg aatactgggt accatgaacc agcagatatc cgcaaacggc
     4561 tagaaaatat cattagtgac aaagtagatg atacggtgac tgttctgctg cctttttata
     4621 cagatttcgg caaacatatt agtataggga aaaatgtctt tatcaatcgt caagtgatgt
     4681 tcgttgactt aggtggcatc tgtttagaag attcagtatt gattggtcct cgtgtcaacc
     4741 tcatcacggt gaaccatcta accgatccaa aagaacgaag aggcttgtca gtaaagccaa
     4801 tccacatcaa gaaaaatgct tggatcggag caggagcaac tattctgcca ggcgtaacga
     4861 ttggcgagaa tgccattgtt gcagcgaatg caactgttac gaaagatgtc ccagacaata
     4921 ccatcgttgc aggaatccct gccaaaatcg tcaaacctgt cgaacggatt atttgaacag
     4981 taagatgaaa taagagatag tgagtataag caagagattt ttcaaaacag gaggagaata
     5041 gaatgaatga tttatcagaa aaagtgattg tcatcatggg cgcttcaagc ggaatcggtg
     5101 aagcaacaac gaagctatta gcagaaaaag gggctaaact agttatagct gcacgccgag
     5161 aagatcgcct aaaagcaata aaagaatctt taccagaagc tgagctatat attcaaacag
     5221 cagatgtaag agattttgca caagtccaag cagttatcga tttagcgatg gaaaagttcg
     5281 gtcgaatcga tgtattgtac aataatgcag gaatcatgcc aaccgctcca ttagttgaag
     5341 gtcaccgtga tgaatggcaa aatatgcttg atatcaacat catgggtgtg ttaaatggaa
     5401 tttccgccgt acttccaatc atggaaaaac aaaaatctgg gcatatcatt tcaactgatt
     5461 ctgtggcggg acatgttgtt tatcctgatt ccgccgttta ttgcggaaca aaatttgctg
     5521 tccgtgcaat catggaagga ttacgtcaag agcaacggca aaataacatc aaatctacga
     5581 tcatctctcc aggagctgtt caaactgaat tatatcaaac catcagcgac aaaaaggctg
     5641 ctcttgaact tcatgaagcg caaaaagaat ggggattaac atctgaagat atagcttctg
     5701 ctgtagcatt tgcgatagaa acgccagacc ggatgtctgt cagcaacatg attatccgtc
     5761 cgacgacaca ggaagtataa tccataacgg ataatatcca gagagaatta cttgttaaat
     5821 agttattcaa acgaccatca atttaaggcg tgaatggaga agaatattct tcatttacgt
     5881 ctttttatgc agaaaaagca aatttattaa aaagaaacaa atgttattta aatatggaca
     5941 ttgacttgct tttacttttt tagttacact gttatttagg aaaagtttca taattcaaag
     6001 gagaaaaaag atgaaagcaa agaatttatt atttagcgtt attcttttag gaaatctagc
     6061 agttgccaac acaacagtgc tagctgaaga atcgacagaa actgtcgcaa ctcagacatc
     6121 gagttcttca gaaacaggaa ttgaaaacga tacagaaagt actttgcagt cagctactgt
     6181 ctcagaaaat acaatttcaa ccgaagaacc aacaactgaa acaacagaac aaacacaaac
     6241 cgaagacagt acatcagcag aagctcagaa caatccagag aatgcagaag aaatcacgaa
     6301 ccaattgaca gatgggcaaa cattgacaca tacccatcaa gcagatttgt ctgaattcga
     6361 aaactatact ggctatgctt tttatgctcg ctcatctgct aattctcaac aagcgttcat
     6421 tgatagtatt gcctcaactg cacaaagttt ggcaagtgcc aatgacttat atgcatcagt
     6481 catgattgct caagccatcg ttgaaagtgg ctgggggaac agtactttgg cttctgcccc
     6541 taattacaat ctatttggga ttaaaggaag ctataatggc caatctgtta cgatgcctac
     6601 ttcggaatat gtgaatgggc aatggattac ggtgaatgcc gcttttagaa aatatccttc
     6661 gtacaaagaa tcattgcaag ataacgtaac ggtattgaag acgacttctt ttcagccagg
     6721 cgtttactac tattccggtg catggaaaag caatacgaac tcttacaaag atgctacggc
     6781 ttggctaaca ggtcgttatg ccactgcgcc taattatggt tctacattga acaatgtaat
     6841 cgaaacctat aatctgacac agtatgatac agcaccaact tcaacttctc cgatgtatcg
     6901 tctgtataac cgtcatacag gagaacattt atatacattg aatgccggag aaaaaaatta
     6961 tctgccaact gtaggctggg aatatgaagg gattgcttgg caagcaccaa acagcggaca
     7021 acctgtctac cgtttataca atccttacag tggagaccat cattacacaa tggcacaatc
     7081 tgagatcaac tctttaacaa aaatcggttg gcgttatgaa ggtctaagtt tttattctgg
     7141 tggttccaag ccaatttatc gactatttaa tccaaatgaa aaaactggta cccatcacta
     7201 taccttgagt gctaaggaaa gagattggct aacaccgatg ggctggagat atgaaggaat
     7261 aggattttat ggttattaaa aagaggtagt gaaaaagctg ccctgcatca agtagtaaga
     7321 acagccaaac aaaaacagat tttcatgttt tt
//
LOCUS       DBNICI010000138         7316 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.139, whole genome shotgun sequence.
ACCESSION   DBNICI010000138 DBNICI010000000
VERSION     DBNICI010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.139"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(98..358)
                     /locus_tag="AC1ZCC_002285"
     CDS             complement(98..358)
                     /locus_tag="AC1ZCC_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HIQ1630513.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(385..1686)
                     /locus_tag="AC1ZCC_002286"
     CDS             complement(385..1686)
                     /locus_tag="AC1ZCC_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIQ1630514.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(1978..2436)
                     /locus_tag="AC1ZCC_002287"
     CDS             complement(1978..2436)
                     /locus_tag="AC1ZCC_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HIQ1630515.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(2449..3780)
                     /locus_tag="AC1ZCC_002288"
     CDS             complement(2449..3780)
                     /locus_tag="AC1ZCC_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HIQ1630516.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(3791..5233)
                     /locus_tag="AC1ZCC_002289"
     CDS             complement(3791..5233)
                     /locus_tag="AC1ZCC_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIQ1630517.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(5220..5984)
                     /locus_tag="AC1ZCC_002290"
     CDS             complement(5220..5984)
                     /locus_tag="AC1ZCC_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HIQ1630518.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(6088..6963)
                     /locus_tag="AC1ZCC_002291"
     CDS             complement(6088..6963)
                     /locus_tag="AC1ZCC_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HIQ1630519.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(7218..7302)
                     /locus_tag="AC1ZCC_002292"
     tRNA            complement(7218..7302)
                     /locus_tag="AC1ZCC_002292"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7266..7268),aa:Leu,seq:caa)
BASE COUNT         2365 a         1531 c         1079 g         2341 t
ORIGIN      
        1 taagccaaca tttcacaaaa atttaagaag caattttcgt aaatttcttc ttatttcttg
       61 aagctaacca cttctgttac aaccactttt agaacattta aagcttcgat gtgacaaacc
      121 atctatgctg ctcgatcata aaattgatag caaaaattcc acaaagtaca tatgtccccc
      181 atgaaaacaa aacattcata tgcggtacaa tgatctttgg aagaatcacg atcaaaaatg
      241 atgccagtgc ccaaatccat cgattgattt gaatatgata tgtagctagc atattcaata
      301 taaggtgact agccgttaat aaaaagataa aaacaaaaaa taaaatcagc caatacattg
      361 tttcccacct tctaaaaacc aaacttagac agtctggact tcgttttctc cacgactttg
      421 ttcatctgac agcatctttt tatcataagc ttttacaaat ggataataga taataaatgc
      481 tatcaatgtg ttgatcatga tcaagataac aacacgccag tcatttcctg ctgaaagata
      541 accgccgatt ggagctggta atgtccatgt tggcaaaaca gtaaatctat tgaccatttc
      601 taatttgact gcaaaataac tgataatagt caaaactaaa ggaactaagt tgaacgggat
      661 gaacatgtaa ggattcaaca tcataggagc tccaaaaatt atgggctcat tgatattgaa
      721 gataccagga ataatagaaa aacgaccaac ctctttcaag aattttgatt tacagaagaa
      781 tataaacaaa acagatacca cgatcgttgc tcctgcacca ccaattgtta ccgtccattg
      841 ataaaactgt tcaggagcga tatagggtaa ttgggtggca gctgtaccat ctgccaatgc
      901 ctttgcattc tcatccaaca tgatcagcca cattggacga acaacagccc ctacaatact
      961 catgccatgg ataccaaatg accacaacaa atggatcaaa aacattggta gtaaaacacc
     1021 aagcaaatta ttccctaaaa tattggttaa aggctcgaat aacgataata aggcagcgtt
     1081 tacatcaaag cccgcgacgc ttcgaatcag ccaagtaatt gttaaaatga ctgcacctgg
     1141 aatcaatgcc tcaaaactac gggcaacagc aggtggcact tgttctggca ttttgatagt
     1201 aatatttcga tttttaaaga aacgatatac ttccactgca aaaatcatag ataagatccc
     1261 cgcaaacatc ccagatgctc ccaaagaact cataggtaag acccagccaa gaggtgcctc
     1321 ttcaggagtt aaaacgttta caggaacttg taacataaag aacgttccta aagataatat
     1381 accacctgtc acactatcta atccatatga tttagctaga tccgaaccca taacaaaagt
     1441 tgcataaata gccatcaatc ccatcgtcat cctaaagggt aaaacaatcg ttgccgaata
     1501 aggggcaata aattcattcc atccaggaaa cggtaaattg ataaagacca taaaaaatgt
     1561 cccaatcaaa atcaatgaca atgttgaaac aaccccatta cgaattgcta ataaatgacg
     1621 ctgtgtacca atttttgcca ttggtgggac aagtttctct tcgacccatt tcatcagctt
     1681 ttccactaaa accactcctt ttcaataaaa tatgaaccat tcattgcatg acaacgcaaa
     1741 gaatgttcat aactaattat ataattaaga aattttattt caatatattc tcttaaataa
     1801 tcaaatttat ttttaagaat cctgaaaata agttttctaa atcataggag caacaaaaaa
     1861 attcgataat taacaaaaat caaaatgatt aaatctagtg attgaaacat atcatttcaa
     1921 aaaaattttg taaattcacc cataaatatc accctattta acttttttca tcgtacacta
     1981 aaattcaaaa caactatctt taatctttac ttcttggttt tgttttaata aatttccttc
     2041 aaaaatgcct ggagaaacta caatgatcac cgtatctttt tgtgcttgct ccaacttctt
     2101 caaatctata atagctaaca gtgtcccttc ttttacctcc tggttacttt caacccgtat
     2161 ctgaaacgga cttccttcga gttcaactgt atctaaaccc atatggatca gtatttttct
     2221 gccagattca ctttcgatcg ttatggcatg tcgggtagga aatatattat cgattattcc
     2281 tttaactggc gcatggatat atccatcatg attcgttata gcgtatccaa ctcctatcat
     2341 ttttgaagaa aagacctcgt cttttactgc tgttaatgga ataatttgac cactagctac
     2401 agcttttaga atgtttcttg ttttaaagaa atgcactttt tatcacctct actttaatgc
     2461 tggccaatat tttttgttta ctttgatcat ttcatctagt attttttttg cgactctggc
     2521 atttggtacg gtttgattca atgtaaatgc ttgtaaagct ttttgataag aaccttcaaa
     2581 gtaagcatct actagtaact tctctactgc aacttgtgcc tccatcaatc ctttatgaaa
     2641 atcactgatt ggttttctta gtgtaattgg ctccacacca gttgcaccaa catatgccgg
     2701 tatttcaaca actgcatctg accgtagatt cggaatcgcc ccttgattag gaacgattag
     2761 cataaagcga cgacgatcat cattcaacaa ggaaatagct atatcgacga tatattgtcc
     2821 atgctcccca aaattaaagt tcaatacttc ttccacaata ccgcggcgta ttttatcagc
     2881 catttctttg gtatttttct ctcgtccatc catgaccata ttagcacgtg tatactcttt
     2941 gtcagtatgc tcaacgacca tatcaggata taagtaatac tctaagtaat tattcgggat
     3001 attacttgga aaatttttcg tgatgactga catcatttga aaggtttttt gccaagtttt
     3061 atctcctata ttaaaatcgg cgacttgcat ttcttttgtt attagttttt caataatctc
     3121 tggcataatt tctcgttgca attctttatc ataaatcgat gtataccagc caaagtggtt
     3181 taaaccataa tacgtaacga tccaattctt acgatcataa ccgtaattca ctgcaatcgt
     3241 ttcttcaatt gaaatagtca tatcacaggc attaatcatt tttacattcg gatatttacg
     3301 acgaacagct tcagaaacga tcgactctgg atttgtataa ttcaaaatcc atgctttggg
     3361 ggcatagtct tgtatatgat cgacaatttc caataaacct ttgattgagc gcatcccata
     3421 agcaaatcct ccgagtccac acgtttcttg tcctactaat ccatgtttaa gtggaatctt
     3481 ttcgtccttt tctcgcatct ccaatcctcc aacacgaatt tgtgagaaaa caaaatcgca
     3541 tcctgtaaaa gcaagctttg gatcatccgt tgcacgaatg cgaatatttg aaaaaccttt
     3601 tcttttcagc ataaatttaa caatcaagta catatccata tttctttctt catcaatatc
     3661 atataaacgt atttcagata atggcaaccg tttttcattg ttcaaaatgg cttgaacgat
     3721 tcctggtgta tacgtactcc cgcctccagc tatagtgata atctgttttt tcatcatcat
     3781 cttttccttt ctattcttct aataatttat tgaattctgc cataacataa gtcacttcta
     3841 agccgatgat aatttgataa tttttttcag ttggtttaat gatcccgctt atccctaatt
     3901 gcttgattcg ctgttcctcg accatggtgg gatccactac ctctacccgt aggcgtgtat
     3961 tacaactata gacatccagg atattggctt ttcctccaaa cgcttgtagc atttttgttg
     4021 ccatatattc ataattttta cttgctaacc ctaactctgt ttcttctaag gttacttcat
     4081 tgccaaactc tgccttagct tcacgtccta atgtctgaat gtttctctta ttgatcaaat
     4141 aataaaaagt aacgtaatac aaggcaaaaa agaaaattcc gattggaaga atcaaaatag
     4201 cattggtagc gattcgaaaa ttgatgagat aatcaacaat acatgctccg aatgtaaaac
     4261 caattcgtat attgaataag taacagacaa tccctgctaa accggcaaaa agtgcatgga
     4321 tcacgtacaa cataggagaa agaaacatga aactaaattc aacaggctca gtgatatttg
     4381 caataaatga agttcctgca ccgcttccca tgattccttt gaccatctct ttattttctt
     4441 tataagctgc tttatagata gctagacata tagctggtat accaaacatc attactacaa
     4501 aaaaaactga taaaaacagt ccagcctgag gatctccgtt gataaagcgt gtcatctctc
     4561 ctctgaaaac ttccttgcta ggagatgtat agcttcctaa atcgtaataa atatacgtat
     4621 tcaacacatg gtgcagtcca aaggggatca gcaaacgatt caaaaacata aatatcccca
     4681 cacctaatgc tcccatatca acgagcagtt ttgcaaaatt atcaatccca atttggatga
     4741 gcggccagat aaagcccaaa aaaacagctg cgattgtttg tagaatcatc accatagtca
     4801 atgggaattt ttcgcccgca aaataagaaa aaactactgg taatttttgt tctttaaagc
     4861 gattatacgt ccatgccgct actaaaccgg aaataattcc accaaaaatc cccatattca
     4921 cttctgggtc cataatttct aagccttttt tcaaggtagc aatcgctaaa aaaccagtaa
     4981 gtgcaggtat tcctttgtcc ttatttttga caaaaccgat tgcaactcct atagcaaata
     5041 agatatccaa gtttccaaaa acggcatttc cagctgcacc aattactggg atgttcagca
     5101 tatcgtcagc tgataaacgc atgatcaatc ccgctaatgg catcgctgca actggaagta
     5161 gcatcgctct tccaagcttc tgcacatttg atttaacatt catttttatt cctcctctat
     5221 taaatatcct catatgttac taactcaatc aaaggatcat ttttcacttt ttgagcaaat
     5281 gctgaatgaa ttaaaaattc gacttggtgg gtacgaggat acacaaaact tgaaccattt
     5341 aacaattcct gatccaaata cgctgcgtga cacatgactt cgatcgtttt atcttctctc
     5401 aatatcatga gtaaacttgc caaatattct tccacactcg tattgatttg tcgttttttt
     5461 tcatcagttt ctcctacgac atcgaaacaa ggctcaaaat aatcgacatg acgaacttca
     5521 tcttttcgtt caaagttgct ccgaaccggc aaatcatatt tttttgctaa tgtaacaact
     5581 acctcctgat tttcatccaa agtatgagta tgatgatgac tgtctaaatg agttggtatg
     5641 atccccgcat gataaatttt ttgaatctga gcattccatt cctgatacag ttgatctttg
     5701 tcaatgttaa atggatgctg ataataagat aaggatctga actctccgct ttcgataaga
     5761 gtatcgacag ttttgaggag tggtttgcca cacgtcaatg ttaaatggac tcctacacct
     5821 agcctaggca tttcctttct tagtttcaca gcatgctcga aacccggcat atttgccatc
     5881 aatgtagttg atgtcaaaat tcctcgctga taagcatcca taatcccata attcacacca
     5941 tggctatagc caaaatcatc cgaattaaaa atcacttttc ccatttcatt ctccttcttt
     6001 tttctactaa aataggcgca accaaataat catagtccat ttactatgtg tcatttggtt
     6061 gcgcctatag atagttacgc ccttatttta atagattctt gctagatgaa ttgccaaata
     6121 gatgatttca ttttcttgaa tggcaagttg aaattgctgt tcgatgatat ctttaatctt
     6181 tagtgctgta caatacgctt tgctatgttt aaatgtttca gtaaaaaaac caatattttc
     6241 tactggcgtt tcttcctgat aaatcaagcg tttaaccagg aatttgatat gaaccattaa
     6301 gcgatgataa gtcagtgatt ctttatcata aacgtttgaa taggattttt ctagatggga
     6361 caaaatcgtg gtcgtcattt ctactatttt taagctcttt aaactattag gctctcctag
     6421 ctcagcatta atgatatgca tagtgatgaa tcctacttca tttgacccta actcggtttg
     6481 atatcgatga ttgattcgct cgaccacttc tactgctata tcatattcat ttggataaag
     6541 gaatctcatt tctggcaaca taattgctgt gacatctcta tcttctcttt ttcgttgaat
     6601 agcaaaataa atatgatctg tcaatgtaat atagatattt gaatctaatg gagaggagat
     6661 cctagctgtt atcagttcaa tcacttcttc cgcaaaatcc ataaattcga ttggaatctg
     6721 cgagactatc tcactgaatc gtttgatctt ttctttagaa tctaatacaa ataatttgtc
     6781 agcatcctca acatcaaatg gcttactcac tttagcagaa aagccaatcc ctttccccat
     6841 cgcaattttt tctattccat cgtcatcgat caatacagca ttatgattca gctttttcat
     6901 cacatacacc gttaattcac ctcctctata tgataaaaca aaacaacttg cagactcctt
     6961 catacatatt tttgtctcca aacaattttt aaagacaaaa aacgacctaa gaaagaaccc
     7021 tttcttaggt cgtgcctaat tgaatagtag caccctacat aatttacaaa aatagtttaa
     7081 cacataataa aagcgttatc aatatctttt ttactcgttt tatgataata cttgtatcga
     7141 attgatttgg aggtaattac ataaaaacaa aaaaaatata gcaactctct ataagaaagt
     7201 tgctatattg ataattatac cggcggccgg ggtcgaaccg gcacgtcctc aacggacact
     7261 ggattttgag tccagcgcgt ctgccaattc cgccacgccg gcaaatttaa aaataa
//
LOCUS       DBNICI010000139         6757 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.140, whole genome shotgun sequence.
ACCESSION   DBNICI010000139 DBNICI010000000
VERSION     DBNICI010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.140"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(767..1060)
                     /locus_tag="AC1ZCC_002293"
     CDS             complement(767..1060)
                     /locus_tag="AC1ZCC_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HIQ1630520.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            1097..1282
                     /locus_tag="AC1ZCC_002294"
     CDS             1097..1282
                     /locus_tag="AC1ZCC_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630521.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            complement(1463..2641)
                     /locus_tag="AC1ZCC_002295"
     CDS             complement(1463..2641)
                     /locus_tag="AC1ZCC_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIQ1630522.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2745..3059)
                     /locus_tag="AC1ZCC_002296"
     CDS             complement(2745..3059)
                     /locus_tag="AC1ZCC_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1630523.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3166..3381
                     /locus_tag="AC1ZCC_002297"
     CDS             3166..3381
                     /locus_tag="AC1ZCC_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630524.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(3681..3923)
                     /locus_tag="AC1ZCC_002298"
     CDS             complement(3681..3923)
                     /locus_tag="AC1ZCC_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1630525.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(3910..4338)
                     /locus_tag="AC1ZCC_002299"
     CDS             complement(3910..4338)
                     /locus_tag="AC1ZCC_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIQ1630526.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            complement(4490..6037)
                     /gene="tnpC"
                     /locus_tag="AC1ZCC_002300"
     CDS             complement(4490..6037)
                     /gene="tnpC"
                     /locus_tag="AC1ZCC_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HIQ1630527.1"
                     /translation="METTDTLLQLLQEAHKTNQTQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKTDKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(6139..6492)
                     /gene="tnpB"
                     /locus_tag="AC1ZCC_002301"
     CDS             complement(6139..6492)
                     /gene="tnpB"
                     /locus_tag="AC1ZCC_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HIQ1630528.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(6482..6676)
                     /locus_tag="AC1ZCC_002302"
     CDS             complement(6482..6676)
                     /locus_tag="AC1ZCC_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630529.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT         2050 a         1320 c         1083 g         2304 t
ORIGIN      
        1 tatagaaagt ttggggcttt ttttctcttt ttattctaag gttattttag tcttgtcctt
       61 taatacaaag attttatttt taaataaaaa atactctttt tttacgatta ttgaaaaaaa
      121 aagataaaac ttgattaatt gaagtattgc ttaaatttgg aagctatgag ggaagaataa
      181 aagagttagg acgggattat ctactatttt tctttgtttt aaatatttgt tatctttgaa
      241 cataaaagag aaatgtgtcg actgcagtgt tgtgtccctc atcacgaata aacggtgttc
      301 aattagctga tcttgtattc acttaaaaaa tggtaaagta cgagttgcta ttccccattt
      361 tcaattttct aacgctcagg acaaaatact gttttcacaa cctcattgga ataaaagcat
      421 atatattgtt tcaagaacga aatcttgtta tctttttaaa gggcatctgt ccaaaatcta
      481 attttaggag gtacgttttg gtatttattc gtacaaataa aagagaatta tctttattca
      541 tgtaacgcat ccaattgatt ttttcatgta cgctcctgga acgattttac tgacgattgc
      601 ttatttaact gcgaatcgct cattagcttt tatcatagct atccatattt tgaataacgt
      661 tcttggcttt gtattataga tacagatgac aaaaaaagtt caagtatgcg gtgtagaacc
      721 aatatcgcat acttgaactt ttttcaatga gtataaatta aaacttctaa ttttcaattc
      781 ccatcatctg atatgcccga ccgtctgaac ttcttttgaa aaagccaaat gtgattagat
      841 aacgtctttg ttcgacataa tcgccactag aaaatacgtt cagcagtatc gaattaacct
      901 ctgtttctgt ataagtcact ccatggtcaa agcgtttcaa gacttctttc aataggatgt
      961 atttcttttt tgttgaactt ggtacttgtt ttacttttcc atcattcatg aatgtgtcta
     1021 aaactttcat cttttcttgc tccgtataag tataaaccat cttctttctc cttttgattc
     1081 taagaatact ccatttatgt tagaatattc tggatttttt attaaagcgg ttactcttaa
     1141 gaaagatatt atcatagtgc tctttttttt actactactt ataggtagat ttattctaat
     1201 ccattattct cccctaccaa taattaaaga aaaaactgga tcagggtgtt caaattacat
     1261 gctaagaaaa aagaaccctt gaataccaag ggttctttct cagtctgata acaatgatta
     1321 acgacggatt tctttgatac ttgtgaaaag tcgttaaagt attgatataa aaggattttt
     1381 attacctctg actacatatt gactaatttg ttaaaaaatc gtttttcaag aatcctgtaa
     1441 taccagtgct ttcaccgttt ttttaactct ctaaagaact aaaagcaatc agaggtttta
     1501 ccttttcatt tgacgttaag tggcgataaa ttttcgaagt tgtttggtca gtatggccta
     1561 agcgatcttt caagacttcc cagctacagt gttccgctac taagcttgcc attgtatgac
     1621 gagctaaatg agtatgaaat ggcttattaa ttcctgagcg attcacgcac cgtttgacgt
     1681 gatcatttac atactctcga cgataaggaa caccacgttt actagaatca agatacacaa
     1741 acaaatatgt ttcttctata ataaacctgt aatgttgcct catctcaccc aaagccttaa
     1801 tcaactgttg tagcttttcc ataactagag gagttactag aactcgtcta gcagaactaa
     1861 ctgtcttagt agtttcaatt cgaaaatcct tactcttacc tttaactgcc gtttgcttat
     1921 agaacagaat ttcatttctt ttgaaatcta tatctccagg ctgtagagca agcgcctcgc
     1981 tgactcggca gccagtgtaa aatagaacat ggaaaagtac agacatcggt aattcctcat
     2041 gaacaatgcc ataattcaaa aaagttctca attcatcgac agtaagaaac ttgtcctcaa
     2101 tattatttat ggcttctttt aaatcagtta ccgttaactg atattttggc attctcacag
     2161 acttcattgg attttcactt atcaaattct ttctttgaca atatttaaaa atcatattta
     2221 gaacttgaat aagtgattgt aaactatttt tggcatagcc aacatttgtt ccattaacgc
     2281 cacattcctt taaggtaaaa agcaattctt gaaggcactc tggtgtcaaa tcagacaact
     2341 ttttatcacc aatccatcga acaataattt ttaattgttc ctctcgctta tcataggtta
     2401 tttccttttg ttcatttaac gaacggtaat attggaacca ttgctcacct acaacactaa
     2461 acttgtcatt gcttgcaaca atttttttct taagcctttg ttgtttctcc atttccagat
     2521 aagcgagttg cgcctcacgt tttgaattaa agccacgtct tgttgttcga tatgcttgaa
     2581 agtaccatgt gatcctacca gtagaactga tttgcttctt gaatcgatct gttttttcca
     2641 tttattgatt cctcctattt tgaaggaacc caacaagcag aaaccctttg gaaatctttc
     2701 tatctatgtc atcattgtac cgcatcttag aaagaaaatg taacttataa agatcgaagt
     2761 agtcgcaatt tttctcgata gtcattcgaa tcactctttc tcatccattc taaaacatca
     2821 tcacgaaaat agtgtcctcg aatcccgtac caacagggag gaaactcacc aagttttact
     2881 aaacggttca gtgtactatc acttatctga aatagtcgct ttaaatcagt atttgttaat
     2941 accagaggta aatgactgac tgcaagcaat ttttccgtgt aatcttccag ttttttttgt
     3001 aaaattgaat agaactctat ttccagagaa ttaacatttg ttttaacttc caactccatc
     3061 acccttctct ctcaaaatca aaacgaccac cacggtaccc cttaaaattg taaaaaaaca
     3121 ctccagtact ccatcaaaac ggactagaaa agaggaattt ttaatatgaa acaaggaatt
     3181 gcagacataa aaataattaa agaaattctt gaaaaatcta cggctaatgc aatagcattc
     3241 ggaacaggaa tcaacttgag tacagtaaaa aaacttaaaa gtggagagag agctgaagaa
     3301 aaattaaatc ttgccgatgc tattaaaatc acagaatttg gcatgaaaaa tatgccaacc
     3361 aaaatcgaaa tatggaaata gtagtttttc tgatctatca gctgttttgc caaagcacat
     3421 aggaatctca cctcccccca gtaatggcgg gccgatttgg ttacggaagt atcattatcg
     3481 tagtcgcgat tctcatatta aaatttgtcg cttaaaagct aaccttgtca tggcgtaact
     3541 agagaatcaa aatctacatg tactgataga tttgctattc atttgtcaaa gagcgctagg
     3601 ctattttcag cctatggaaa tcctttattt tgacccgata aaaaacttcc atagaatgaa
     3661 aaggaaaggg aaaaaatact ttaacgagtt gtatcaaatt caagtatctt ctcaatcaat
     3721 tttgtttcca gttgtctacg catatattca tctacataca taaccattcc tttttcttct
     3781 tcataaactg gtttcatggc taaccgtgaa atataatttt gatattttga cagaacgatg
     3841 gacatggcaa ttgaatcacc agaagctgcg gctttgattg tcgttagtgg tatgaatggt
     3901 atatatcgtt tattttgctt catatttgac acccaaatac tttcctagct cttccaaagc
     3961 catttgtcgt tggtaccaaa cactgccgag acttttccca ataatatcgc caatttcacg
     4021 atcattaaga ccgatgaaga aggacataaa aatgatttct tgtctttgac taggtaatgc
     4081 tgcaatcgca tttgccaact cctcattgga aataaagatt ttcataccaa ggacaataaa
     4141 agtaccacta ctattcaaat ttggttccaa ataatagaac tctgtaccta accctttttg
     4201 ttccaatatt tccattgata tttgccgttg gcgaaatcta gcgtacttct tttgaatatt
     4261 taacgcctca ttgcagacta ctttcttaca aaaactgtca aacgtatgtt gaatgtgggt
     4321 ttcctctgag tcgcccataa tattctcccc ctcaaaaaat tcattcaact cgttttactt
     4381 ctatcccttt ccattattac tacaatctag gaggtaagcg ccccatagaa cagtacctgt
     4441 acgtcaaata acccgaccta tagaatagaa tcgggaatgt ttggcgtttt tagttacgag
     4501 aaaaatgttg ttgtacatcc ttgttccaag gcaacagttg atctaaagtt tctgctgaaa
     4561 gtacgggaag atttggtatc ttatcaaata gaaactctag atactttaca ggattcaatc
     4621 cgttaagttc tgctgtttta tatacactca gaatgattcc tgaagttcgt gctccttcaa
     4681 gacttgtgga atgcaaccaa tttttccgtc ctatcaccag ctctttgata tttcgctccg
     4741 cacgattatt tgtgagttct aaacgaccat cttcaaaaat aggttcaaaa ccacttcgta
     4801 gtttacacgc atattgaacg gctgcatcca acttggattt tgcaacagga tctatcgttg
     4861 ccatccactc ataaaaagca ataaataatg gacgcagttc ttgttgccga cagcttaatc
     4921 gcttctcagg agaaaagtct ttccatttct tttctaggac aaaccattta tccaattgtt
     4981 tgactgcttg agctgcggga atatccgtgt ttttctttcc ttttggaatt gcttcgaaaa
     5041 atttccgccg aacatgcgcg aaacaataga caagtcgtac actttccacg gcattatacc
     5101 cgctatagcc gtcacagtgt aaataccctg taaactcctt gagaaaagtt ttcggaacat
     5161 ctccagcacg actttcgtcg tggtgataat aaacgatggg atgctgactg tcttttcctg
     5221 tacaaaactg ccagtaatag gtatctgttt tggcactttc aatcacacga taacttgttt
     5281 catccccgtg tagaatctct tcttgattaa gatagactct taatcgttct cctaaagggt
     5341 ctaacgcgtt ttgcactgct ttaatatgcc aattggtgat attatctctc gaaatatcca
     5401 acccatgtgt ttgtttccaa tagttctctt gccgataggc aggaactttc tgaccaaatt
     5461 tcatccggat cgtttccgcc actaaactat tggaacctaa gctattagaa atcaaaggtt
     5521 taggaacgat cgctttttta aaagaagaaa gtccttcttt ttcgcagctt ttacaacaat
     5581 aggtatgttg ataatgggtg agacaatcca acatagcctg atggaaacga atttcttctc
     5641 gtacttttgt ccgtccgata tctttcatct gtgaaccgca atgttcacaa aattgttctt
     5701 ccaaaggaag gagatgttct ttctctgtta taggtaagtg tttgattttg tctgttttcg
     5761 ttcctacaac tcgttgatgt ccttttactg gaataatggt ggcagctgct tctggaacac
     5821 ttacagaaat ttcttcatca ctaaaaagat tcaattgccc attcgtttct tcaaagagag
     5881 tctctttaga gcgtccaaat aatttgttgg ttaaataatt gaccttttca ttcaatagtt
     5941 ggatttcagt tgtaagattc tgaatggttt gttgttgtgt ttgattggtt ttatgggctt
     6001 cttgaagcaa ctgaagcaac gtatctgtag tttccaaaat ttttcaccac cttgaatcga
     6061 actgattttg ttacagaata ccacaagaaa cgcacaagaa ataggggttc aacacatttt
     6121 ttgtgttaac cccccagctt aaaaaactcc tttttttcct ggcaaaatag ctcgaggttg
     6181 atcgatactt aatccttcta acaaccatcg aacctgttgg ttcgttaagg ttcgaacttc
     6241 gtctttcgtc cgaggccatt ttaacttgcc gttttctatc cgcttataac agagaagaaa
     6301 accatcaccg tcccaataga gaagtttgaa acggtcttga cggttcccgc aaaataaaaa
     6361 aacggcatct tcgtaaagct cgagttgata ctcttgttga ataagactgg caagaccatc
     6421 aattcctttt cgcaaatctg tttttccgca cacaatataa atatgtttgg tttgaagtaa
     6481 atcataaagc attggacaac agttctttca aaatagcttg aagaatataa ttgttaatac
     6541 cgttatagat caaaacttca gaaccattct ctcttttgat gcgcatcgct aacttggcag
     6601 ccgcgggttt acctttttct ggtgcactct tttcgtttcc taattggaca ggaacaatct
     6661 catttaattt atacatagaa aacctccaga cttcttgata gctcaagtat atcttgaggt
     6721 gcatttgtta gtaaggtact gttctatggg gcgctta
//
LOCUS       DBNICI010000140         6671 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.141, whole genome shotgun sequence.
ACCESSION   DBNICI010000140 DBNICI010000000
VERSION     DBNICI010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.141"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..614
                     /locus_tag="AC1ZCC_002303"
     CDS             <1..614
                     /locus_tag="AC1ZCC_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HIQ1630530.1"
                     /translation="VFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVGK
                     YMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGTG
                     VFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPFI
                     LALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            611..1384
                     /locus_tag="AC1ZCC_002304"
     CDS             611..1384
                     /locus_tag="AC1ZCC_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HIQ1630531.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1381..1779
                     /locus_tag="AC1ZCC_002305"
     CDS             1381..1779
                     /locus_tag="AC1ZCC_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIQ1630532.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1964..2635
                     /locus_tag="AC1ZCC_002306"
     CDS             1964..2635
                     /locus_tag="AC1ZCC_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630533.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2694..3134
                     /locus_tag="AC1ZCC_002307"
     CDS             2694..3134
                     /locus_tag="AC1ZCC_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630534.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3146..3814
                     /locus_tag="AC1ZCC_002308"
     CDS             3146..3814
                     /locus_tag="AC1ZCC_002308"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HIQ1630535.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3878..4501
                     /locus_tag="AC1ZCC_002309"
     CDS             3878..4501
                     /locus_tag="AC1ZCC_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIQ1630536.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4557..4943
                     /locus_tag="AC1ZCC_002310"
     CDS             4557..4943
                     /locus_tag="AC1ZCC_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HIQ1630537.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5156..6547
                     /locus_tag="AC1ZCC_002311"
     CDS             5156..6547
                     /locus_tag="AC1ZCC_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HIQ1630538.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         2123 a         1081 c         1484 g         1983 t
ORIGIN      
        1 ttgtttttgc accgttagaa ggagaagacc tcgcttcatt tctggttggt gcggcaatca
       61 tgatgatcgg aatgacgctc tttttgtttg gagcagatta ttctatgatg gaagtcggag
      121 atctagtagg gaaatatatg atcaaaaaga aaagtttgac cgttttggtc agtttgggat
      181 ttgctatcgg gatcgtgatc acgattgctg aaccttccgt gcaagttttg ggacaacaag
      241 tatatgatat atctgacgga gagattggca gagtgttgct gattgggatc gtcagcgtcg
      301 gcactggagt atttctagcc tttgcgctgc ttcgggtcgt attcaagcta tcctattatc
      361 aattgatggc tataggctac ataggcgttt tgatcgcctc atttttcacc agttccgaat
      421 ttatgccgat tgcttttgat tcggggggag tgactactgg tccagtaact gttccattta
      481 tcttggccct tgccggaggg atgactagta tgatcaaaca aaagaaaaat gaaaatgaca
      541 gttttggaat ggtcggtatt gcttcgttag ggccgattct tgccgtgatg attttggggg
      601 tgatcttcca atgagtttct ggcagcaagt gatagatgga atcgaaatcg ttaccatgga
      661 agtcattttg gcagtggcac ctatcgtaat cctatttgtt ttaatgaata tcattagttt
      721 tcgtatgcgc aaaaagcgtt tctttgacat tatgaaaggt ttcttgataa cgactattgg
      781 gttgatcctt ttcctgcacg gagtcaatat ggcttatgta ccggtaggac agcatttagg
      841 cagtgctatt gcgggattgc cttacaatta tatactggtt ccccttggct ttttaatggg
      901 gttcttagta gggtttgctg agccggcgat tcatgtaatg atcaaacagg ttgaagaatt
      961 gagcgagggg agaatccgat cgaaaatcat gctgatggta atttcaattg ggatcggcgc
     1021 agcagtagga ttgtctatgt ggcgtttgct cgtcggattc tctctttatt atttcttgat
     1081 cccaggatac gggctagcat ttattttagg acgaaaagtg gacaagatct ttttagcaat
     1141 ggcttttgat aacggtggag tagcaacggg cccgatgtgt tcgacattta ttttatcaat
     1201 gagcgtggca atcgctgcac aaattgaagg aagagatccg atcattgacg ggtttggtgt
     1261 ggttgcaatg attgcattga caccaatatt atccacttta gtactaggat atatttataa
     1321 acgaaaagat gaaaaagaca gaatcgaaaa agaaagattg caaaaattgg gggaaagcaa
     1381 atgaaagatc ttgtagcatt gaatcttcac atgattgtga cgatcgttga cggaggaatg
     1441 ggcgaagatg ttatcgactt ttcaaaagaa gcaggtgcag caggcggaac gatcttacat
     1501 ggaagaggtt ctggtgtaca tgattcaggg aaatttttag gtttggacat ttcgccagaa
     1561 aaagatattg tgttgacatt agtacccgat agtttgacga ataaagtaat ggatgcgatt
     1621 ggtcaaggca tcaacatcga tattccaggt aatggtattt gttttagtat tgatgtagac
     1681 aaagtcattg gtatcactaa gattcatcaa tatcgagaaa ttatcggaat gcaagaactt
     1741 ttagatgaaa ataacgatag agaaaagtct gaatcataga caagcgtttg aagaggaaat
     1801 aaacacaaac aagaaggcgg atgagcgtta tttccgcttt tttgtttgta tttttctcgt
     1861 gaatacttat gaaatagtaa atttttatga aattctactc taagcatcag actttctttt
     1921 caaaatatgc tattttaaaa atgatgaaaa aagggggaga aaaatgaatt ttttagttgt
     1981 tttaaaagat actctgattg agcgatttac atttctatat gttcaattgc aggaaaataa
     2041 aacattgctg catttttcac cagagttcca tctagagggg ctaacattag gagaaatcta
     2101 tcaagcagaa gggctgtcgg ctgttttgca cttttttaag gtagaattgg aattaccagt
     2161 aaaagattat atcgagcttt ctcttgaatc ttgggatcga gcagtggttg aactgtttcc
     2221 agaaggttta atgatcgata caaacgatgg gaagatccat ctgactgcag cagaactgca
     2281 aaaacaaatg cgttatcaaa tagatgacga aaattctttt tctattttta gacgtcagca
     2341 aaagattcta aaaagctttt taaaagtgat cagtaaaaaa cggaatctga tcaaaacgac
     2401 acagctgttg aaaaaatatc caggtctgct tcacacgtct attcaatttc ctcaattgat
     2461 tacgatgatc catcgtttta tacgtatgca acagctacca tctaagaagc tgtttcttcc
     2521 attgacggac acatttcgag tagtcaacag agaagataaa aagaaagtga ttgtgattga
     2581 ctttaccaga aatcggaatg tccttcacca agttgcaatg gaaaaggcca attaaggctt
     2641 tttctttttg gatttttttg tcataataaa tattagatga tggaggagat aatatgatgc
     2701 aaacatggtt agaaaggcta atacgatttc agcagaacta tcacaaactt atgcagggaa
     2761 gatatgcgaa atttgatcag ttgaatcgcg gtctattgat cgctgcgctt gtctttagtt
     2821 tgctaagtcg ttggttgccc tatagaatcg gacaattgtt gttcatcgtc tttttcggat
     2881 ggatgatatt tcgtttccta tcaaaaaaaa tctaccccag actaaatgag aatcaacgtt
     2941 atttgaaata tcttgataga gtcaaagaaa aatatcatca gataaaaaac aaaaagcaag
     3001 tcaaaatgga tcgaaaaagt tatacttttt ttgagtgccc gaattgtcat caaaagcagc
     3061 gtgcacctaa aggaaaagga cgaatacgtg tgacatgcaa aacatgtggt ataaaattcg
     3121 aaacgaatgt ttaagggaga aaagaatgaa attagtatta ttacgtcatg gcgaaagcga
     3181 agcaaatttt gaaaattact ggacaggatg gctagatgtc gccttaacag aaaaaggaaa
     3241 agaacaagca gcagttgccg gtcaaaaaat gcttgaagcc ggacttcaat tcgatgccgt
     3301 ttatacctct gttttgaaac gtgcgattct taccggacaa gtcgcactag aagaaatggg
     3361 tcagttatgg cttcctataa taaaaacatg gcgtctaaat gaacggcatt atggggatct
     3421 tgtgggtaaa aacaaagata agatgaaaga aatatacgga aaagatcaag taaaaacatg
     3481 gcgcagagga tatacggaag ttccgccgtt aaccgcagaa aatagatttg accgcagata
     3541 tgatcacttg gatccgcatt tgatcccata cggagaaagt ctggaaatga cagtgaatcg
     3601 gatcattccc ttatggcaag atcatctagc accaaaacta aaagatcggc aagatttact
     3661 agttgtgggt catggtaata gcatccgagc actgacaaaa tatttagaag atgttcctga
     3721 agatcaaatg gatacgatcg atatcccaaa tgcacaacca atccagtata cattagcaga
     3781 tgatttgaca attctggaca aaaaaatact ttaaagaaca cctttatcta aaagtgagag
     3841 atagaaaaaa gaagcaaagg aatggaacaa aaggaacatg aaaaattata tatgggactt
     3901 tgatggtaca ttgtttgata cgtatcccgc aatggtagat ggtgcatggc aagcactaaa
     3961 agattttggt atcagtatgg acaaaaaaga aatctacttc aaaatgaaaa agtattctac
     4021 tagttatttg atcaatgaat caaacttgaa tgccagagag tttaatgaat tatttcaccg
     4081 atatgaaaaa gaaagtacgg aagtttcccg tccatttcca gaaacgaaac aagtactgga
     4141 aatgctaaaa gacaacggcg gtcgtcattt catcctgacc caccgtttga cagaatcaac
     4201 atggggcctg ttaaaagaac atcgattggc acatctgatt gaggaagtag tagggatcga
     4261 tcaggacttt cctcgtaaac ctgatccagc atctttaaat tatttgatcg atacttttca
     4321 tttagaaaga acagacacga tgatgatcgg cgatcgtcgt ttagatatcg aagcagggaa
     4381 aaatgcagga gtcgctactt gtctttacga catcgatcat tttctgggtg agatcccggc
     4441 agattatgtg gtaggaaatc tgaatgaaat ccttaccttg cagagcatca ttaaaaagta
     4501 gtatcccctt tcaaaggaga tatgttataa ttttcataaa ggagtgataa aattatatgg
     4561 aaatcagaag acaaaaagaa gttgtagaag catttcatta tgatatgaga ttgccgaacc
     4621 aagaagtaga aacagatttg aaagtaggct tctcgccttt aaaagaaacg atcgaaaact
     4681 atccgaaaga acatagcatc gtaggagcac gcttagagtt ccgtttagtg tttgatacat
     4741 atgttttatc tggaagtgtg agtcagatca atcatatcct caatcggaaa attgaaggtc
     4801 aaaatgatgt cacacaagaa gaagtggacg agctagtagc accgttattc gatatcgtac
     4861 gccgaatgtc ttatgaggtg acagagatcg ccttagatca gcctggggta aaattgaatt
     4921 ttcaatcaga aaaacaagaa tagacagtaa aaacatacct gcgatagatt aaagctatca
     4981 caggtatgtt tttttattac taattgtaag caaaaagaat gtcatttcga taaaaatcgt
     5041 ctaaaatata aaagtcactg aaaaaaattt tttcaggagg atggttgaag aatcattctc
     5101 gggaaccttt acttccttcg atggacttgt cattaataat atgaggagga gaaaaatgac
     5161 aacactcatc acagttttag tggttctagt ttttgcggca ttattatatg tgttctatcg
     5221 tatgcaaaag cgccatacaa aattttcaac acgcgtcttt acagcattag gagttggtat
     5281 cgtctttggc ggtctgcttc aattgatctt tggtgtagac gacaaagtcg ttactcaatc
     5341 aatggaatgg atcgggattg taggtcaagg atatgtatca ttcttgcaaa tgctagttat
     5401 gcctttagtg tttatttcga tcattggtgc attcacaaaa atgaaagttt ctgaaaagct
     5461 agggaaaatt agtgcgactg tattgacttc tttacttgga acaacagcta ttgcagcatt
     5521 gatcggaatt ggttcaacaa tgctgttcgg actccaagga gctaaattca cacaaggagc
     5581 tgcagaaaca gcacgaatct ctgaactggc aacacgtcag gaaacagttg aaaacttatc
     5641 gattccacag cagattttat cctttatccc taaaaacgtt tttgctgatt tagcaggtag
     5701 tcgtgcaaca agtacgattg gagtcgttat tttcgcggca tttgtcggaa tcgcttatct
     5761 aggcgtgaaa agaaaagcgc caaaagaagc agcattcttt tcagatttag tcgacagtct
     5821 gtataaaatc attatgcgga tcgtgacgct tgttttgcgt ttgacacctt atggagtatt
     5881 tgctttgatg acaaacgttt tggcaacaag tgattttgca gctatcgtca atttaggtaa
     5941 atttgtcgtt gcttcttacg tagcattgct tgctgttttg attgtccact cactgatttt
     6001 gattggtgca aaagtcaatc caatcaacta tttcaaaaaa gcctttccgg tattgagctt
     6061 tgcgtttact tcacgttcaa gtgctggagc tttgccattg aatatcgaga ctcagacaaa
     6121 agctttagga gtagatgatg caacagctaa tttctcagca agttttggtt tgtctattgg
     6181 acaaaacggc tgtgctggcg tttatccagc tatgttggca acgatcgtcg caccagcagc
     6241 tggagtgaat gtctttagtt tggaatttat tttgatgctt gtggcaattg taacaatcag
     6301 ttcttttggt gtagctggag tcggtggggg cgcaacattt gcttcactga tcgttttagg
     6361 ggcaatgaac ttgccagtag caatcgttgg gttagttatc tctgttgaac cattaatcga
     6421 tatggcacgt acagcagtaa acgtcagcgg tagcatggta gctggtatcg tgacaagtgc
     6481 acgcatcaaa gatttagatc gcgatgtcat caatgatgat tctaaagtga tcgatgtgaa
     6541 tgcatagtca aaaagaggtt gtgatagaag tggtcagctt taagaaataa gaagaagttt
     6601 cctaaaaata gtttctcaaa tttttgtgaa atttcggctt atttccgaga agctacttct
     6661 gaaacaccgt g
//
LOCUS       DBNICI010000141         6635 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.142, whole genome shotgun sequence.
ACCESSION   DBNICI010000141 DBNICI010000000
VERSION     DBNICI010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.142"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            99..746
                     /locus_tag="AC1ZCC_002312"
                     /pseudo
     CDS             99..746
                     /locus_tag="AC1ZCC_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="AC1ZCC_002313"
     CDS             1139..3784
                     /locus_tag="AC1ZCC_002313"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIQ1630539.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="AC1ZCC_002314"
     CDS             4094..5407
                     /locus_tag="AC1ZCC_002314"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIQ1630540.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="AC1ZCC_002315"
     CDS             5397..6050
                     /locus_tag="AC1ZCC_002315"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIQ1630541.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..>6635
                     /gene="radC"
                     /locus_tag="AC1ZCC_002316"
     CDS             6105..>6635
                     /gene="radC"
                     /locus_tag="AC1ZCC_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HIQ1630542.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHN"
BASE COUNT         2183 a         1131 c         1455 g         1866 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tagcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataat
//
LOCUS       DBNICI010000142         6621 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.143, whole genome shotgun sequence.
ACCESSION   DBNICI010000142 DBNICI010000000
VERSION     DBNICI010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.143"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..1116)
                     /locus_tag="AC1ZCC_002317"
     CDS             complement(<1..1116)
                     /locus_tag="AC1ZCC_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630543.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1349..1471
                     /locus_tag="AC1ZCC_002318"
     CDS             1349..1471
                     /locus_tag="AC1ZCC_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630544.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            1587..2894
                     /locus_tag="AC1ZCC_002319"
     CDS             1587..2894
                     /locus_tag="AC1ZCC_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIQ1630545.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3015..4214
                     /locus_tag="AC1ZCC_002320"
     CDS             3015..4214
                     /locus_tag="AC1ZCC_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIQ1630546.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4241..5509)
                     /locus_tag="AC1ZCC_002321"
     CDS             complement(4241..5509)
                     /locus_tag="AC1ZCC_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HIQ1630547.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     regulatory      complement(5565..5631)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(5679..6320)
                     /locus_tag="AC1ZCC_002322"
     CDS             complement(5679..6320)
                     /locus_tag="AC1ZCC_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
                     /protein_id="HIQ1630548.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(6392..>6621)
                     /locus_tag="AC1ZCC_002323"
     CDS             complement(6392..>6621)
                     /locus_tag="AC1ZCC_002323"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630549.1"
                     /translation="AYFLKKLLLEHRLFVERDFDTTFVTVLFLFAVDYLLKIRFTFIY
                     RMNHVHRRMNIKGGDDKMQNVSYLKESGIFR"
BASE COUNT         2030 a         1395 c         1174 g         2022 t
ORIGIN      
        1 ataacgaaga atcgctgtca gactcttttc atctggcgca tctttttgat ccaaaataaa
       61 cacttttccg ccattaatga tcacatcatc tgctaatgca ttcaacactt gacgccgatc
      121 atattctgtt tcaggatctt caccgaaacc atcagctaag tttgacgtag cgataaataa
      181 atgtgacact tttccttctt tcgcagctgg aacgatatca actaactgat cgatgaattt
      241 cttatctaag agtttattgt attcttttga ttcttgagct gcgagttcac ttgacagctt
      301 cttcgcccct tcttcgatat ctctaaagga tagctgcgca ggagaagcag aaatcgcaac
      361 gtctgcacaa taatagggat ttttagcaat ttttttgaac atcgtctgat tttctggtag
      421 agcaaataaa taaatcggcc attgttctgg attttcgaaa gaatccttca agtacgtatc
      481 aactgcttgg taatagttga cccaatcgat ctctacttct tcatcttttg gatttacgcc
      541 atggaaagtt actccatctc gggctccgcc tcctccatta gaagagaagt tcaaaccgcc
      601 acctgtcagt tcagatccca atgcagtaac gacatcaacc ggtgcccctt ctggtagaga
      661 tacttctttt acttgtttgt tgctgacttc atacaatttc atggagtcgc ggttcaatcc
      721 caataaataa taagaatagt tgaactgccc atttttgata atagccagta aataaggaag
      781 atcacttaca tagtattgat cgtctaccgg gatactcaaa cgatggatat aggtttggtc
      841 agcagttata atgaccgcta cactcgtagt tgcactacgc caaaacgaag gatctcctaa
      901 caaattttca attttttctt ggaacgggga ccagtcatgt tcggaatatt ttttctcaaa
      961 acgtttcttt gcttctttcg caaaattttt gaatttgatc tgatcttttt ccacatcttg
     1021 atgggcgaca tgcgtattca gcatgatcgt gacgaatggt cctttagcgc cttctgaagt
     1081 caattgtgac aataattctt tatccttttt tgccataaac gtacccctcc taaaaaaatc
     1141 attcctttgt ttctatctaa agtctaccac aacactattt ttccatacaa taaaacgtat
     1201 ctaaaaaaat aaaacggttt ctacggtttt aaatttagct atttttttaa gttatcttca
     1261 taatttcctc aattatttag ccccagtctt caactattct tcatgaaatg ctgataaact
     1321 aagaaaaaga ataaaagagg tggatcttat gcatttgatt atgattttca gtttattaag
     1381 tattatcagt ttatttgctt tcagcgcttc ccgcttatta gatgcggaaa cacagcctat
     1441 tcctgtaaaa gtacgtaaaa acgaccgcta accttccata acgtgcgttc ttatcctaaa
     1501 gttttttcag aaacattgaa actctctccc ctctctgata aaattttaat ttgtagcgaa
     1561 acaaattaat tacaaggaga tcggatatgt ccataaaaaa aacacgtcta gtcctattat
     1621 tggctagtct cattctttgc cttgcttctt ttctaccagc tgttgctgtg caagctgaag
     1681 atactttcaa agtaaatgca aaagcagcct ttgctgtaga tgcgcaaagc ggtaagattc
     1741 tttatgatca agatggagaa aaaaccatgg ggatcgcttc aatcacaaaa atcatcggtc
     1801 tttacatcgt ccttgatcaa gtaaaagaag ggaaactatc atgggatgat aaagtttcaa
     1861 tttcagatta tgcagaaaat ttgagtgtca ccccagattt atcaaatgtt cctttgcata
     1921 aagaaaacac ctacaccgtt aaagaattat tcgactctgc aatcatccag tcagccaacg
     1981 cttcaatggt tgccttggca gaaaaaatct ctggcagcga agcaaaattc actgaacgaa
     2041 tgaaagaaca attgaaagat tggggtatca aagatgcaac gatcgttaat gcatcaggac
     2101 tgaataactc ttatcttggc gaaaaccgac cagaaggtac tggagaaaac gacgaaaatc
     2161 aaatgtctgc tcaagatgtg gctatcgttg cccgtcattt gatcttggat tttccagaaa
     2221 tactggatgt atctagcact acgacacaaa tgtttggcga aaacacgcaa tcaccagtag
     2281 aaatggtcaa ctggaactgg atgctcccag gattcgtgaa ttataaagaa ggcgtggacg
     2341 gattaaaaac tggtacgact gaacttgcag gagcctgttt cgtcggtacg atcactaaaa
     2401 acgatcaacg aatcatcact gtcgtattga atgcagacgg ccatgcggaa aacccaagtg
     2461 cccgtttcaa tgaaacagct cgcttaatgg attattgcta cgacaattgg tcagaaaaag
     2521 agcttggaaa agccaatgca agtatcccta gtctaaaaac cattgatgta aaagacggaa
     2581 aagaatcaac agtgcccgtt gtgcttaaaa gtcctgtaaa agtatgggta cggaacgata
     2641 tggatactgg aaaattaaca atcacaccaa ctcttgacaa aaaacaagtc actgacaatg
     2701 agatccaagc acctatggat aaaggaacaa aaatcggtac cgcaacgatc accttggcag
     2761 aagataaatt aggttatctt gaagacaaca acgcgccatc cacagaaatc atcacggata
     2821 aaaccgtaga aaaagcaaat attttcgtta ttggctggcg acgcgtcgca gaatttttcg
     2881 gtaacttgtt ttaattgaac aaaaagaaaa aagcatcttt ccatgtggaa gatgtttttt
     2941 tgctttttct agtttaaata acagcttttt tcatctattt tcgttatact gtataagaaa
     3001 aaggagttga atttatgaat ctaaccagag atctgatttt tttggttctc tatcttattg
     3061 gggtattctc catcgtttat tggcgaaaac agaaaaagga tccttccttg ttcttttaca
     3121 gcagtacatg gacgtttgcg gtaatcttga cttattttgt cgtgttggaa attttttatt
     3181 cgacctttta ttttttgatt cccttagtgt ttttcagtat ctttgccttt tcctattttt
     3241 cagaaaagcg aaggctgttg aatggtcttt tattcaatat tttcctcatt agttttggta
     3301 tctacttgtt tgtcttgcta tacgaaacac aaaatatttt tcttggcgga ctgattgcct
     3361 tgatcacgat cccgttactg cttgtctttc tattcggcat ttatggattg atcgtttttc
     3421 ttttttggaa cggtgtcaca gttttacgta gagagtcgca ttcattagct aatcttttaa
     3481 cgttgatact agccattttt cttacattgt ttcttgtttt tgactttttc ttgttaaaat
     3541 atttaccaca atggataaat gctttgtttt tctgcatccc attgatcttg atctatctat
     3601 ttatcgtatt ctacaacttt ttgactgtct cattcttgta tcagttcaac cgccctcgat
     3661 acaaccaaga cttcatcgtt gtgttaggcg ccgggttgat caatggagaa acagtttctc
     3721 ctcttcttgc caagcgaatc aataaggcga ttgcttttta ttgggcacaa agccgtgcga
     3781 cattaaatcc gcctatccta ttaatgtcgg gtggacaggg agcagacgaa aaagtaccag
     3841 aagcaatggc gatgaaacaa tatgccatgg aacaaggaat tcctgaaaga gatattcttg
     3901 tcgaaaccaa ttcgacaacc actttggaga atatgcgcta ttcaaaggaa atcatggatc
     3961 agcaaatgaa aggtccctat cgcgcgatct tcagttccaa caattaccac attttcagag
     4021 ctggtctcta tgcacgacaa gcgaaactga aggcaaatgg cattggggca aaaacagctt
     4081 tctattactt gcctaacgcg tttttaagag aatacatcgc catcttgatg atgaacaaaa
     4141 aaagacatat gatcatttgc ggattgcttt tcgcaggttc cgcattctta tcactgatca
     4201 ctttactttt ctaaaaaaac aaggtgtgac aaaacatatg tcacaccttg tttttttctt
     4261 ttattctctt gacgattggc aagaatagta ttcctttttt gacggctact tgctgtgcag
     4321 aataaatacc gctgtttcct gaacacataa agctgatcaa gcagatcata aagaagtaag
     4381 cagcagcttg tgaaccaaat aattcgattc ccatcacaaa acaggcaatc ggtgtatttg
     4441 tcgcaccaga aaataccccg ataaaaccta atccagcaag aaaaggaaca gaaacatgga
     4501 gcaaaggcgc tagtactgcg ccaagcgttg caccaatctc aaatagtggt gtcacttctc
     4561 ccccttggta ccccgcacct aaagacaaga ccgtgaagaa taatttccca ataaaatcat
     4621 aagcatgtgc ctgtccatta aatgattcct gcagtaacgg aagactcaac ccaaggtaac
     4681 gttgtgtttg caaaatcaac gctgcggcta caactactgc tccacctatc gtatttcgcc
     4741 aaacaggatg aggaatccat tgcgcatacc gcgcttttaa aaagacgatc gaacggctga
     4801 atacccaacc aatcaaacca aaagcaatcc cagcaacaaa aactttgaag aacagcaaca
     4861 tactccattc aggaatcaca cccatttggt aatgtagatg agaaacacca tagctttctg
     4921 tcatgaaatt tgcaaaaagc gcagcaaaga agctaggaaa tattgcttct gttcggactt
     4981 taccgattgc caacacttct aatccaaata atgttccagc taaaggtgtg ccaaatacag
     5041 aactaaaacc cgcactgatt ccactgataa ccaaaatctc ccgttctgct ttatccaatc
     5101 ggaataaatg cgctatatta tcagctaatg ctccccccat ttgaactgct gttccttcac
     5161 gaccaacaga tcccccaaat aaatgggtag tgatggtccc aaacaaggtc aatggaatga
     5221 gccgcaaagg gatcttctct tctcctccat tcccttgatc gatcactaag ttattcccac
     5281 gactagaaag accgccatga tacgcataaa gataagcaaa aagcgcgccg ctgactggca
     5341 atagataaag tagccaagga tgtgctagtc ggatatctgt cacgaacgtc aggctattta
     5401 agaaaaaagc agacaagctc cccatgaata ctccgagaat cgctgtgacc accagccact
     5461 tcaacatgta catcaaaata cgcactggac tcggtaattg tttttccata aaaaattcct
     5521 cctcaaataa aaaaacgcct actaaagaaa ggggctggcc ccagacttta gtaggcgtca
     5581 ttagctatct tacgttagca tttaaaggcg aacaccatca cctattcact tccattaaaa
     5641 gatagcatat ttttctgtaa aaaacaataa aattatcgtt atttctttac acgttcagca
     5701 aattcataga aaggagccaa tttcgttaac tgttgattac attgatcact atcatgacaa
     5761 atataattcc cctttttcgt atacgtgcca tcagaaccgg ctttcgttgt agaaagaaag
     5821 agcgagacat tcgatgtttt ctgacagatc gcacagattc ccttattaat cgttggtgat
     5881 aaagtaccgc tgatcccttt taattttcca tcttcgtaat aaaccatgaa ttttttttgc
     5941 gtccctggat cattccaacc gatgtacgta tgtgcttgca agtcgatttc attccaagcg
     6001 ggttgtttga gttttttcac ttttcggaat aatttctcga tttgtggttt gctcggctgc
     6061 ataaacggct ggacgatttg cttcagctcc tccaatgctc gttctgcttg cgtttttgtc
     6121 agttttacat ctaaaactgt cgttaaaaac gtatcgattt ctgatggttc gtaacctaat
     6181 acatcgcgaa tcttttcagc tgctagagcc tgaaccgttt gtaccgtttt tgagtcattg
     6241 acagatgagt acgcatttaa taactgctct acttctcttt ttataaaaaa atattgatat
     6301 ggtttcattg tttgttccat gattttaaag tcctttcttc taaccaaagt gcatactttg
     6361 tagaaaaaag tgatcttctg caaagtatgt gttacctgaa gatcccggac tcttttaaat
     6421 aagatacgtt ttgcatctta tcatcacccc ctttaatgtt cattcttcta tgtacatgat
     6481 tcattcgata aataaaagta aaccgtatct tgagcagata gtcaactgca aacagaaaaa
     6541 ggactgtgac aaaagtcgtg tcaaagtccc tttctacaaa caaacggtgt tccagaagta
     6601 gcttcttcaa aaaataagcc g
//
LOCUS       DBNICI010000143         6434 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.144, whole genome shotgun sequence.
ACCESSION   DBNICI010000143 DBNICI010000000
VERSION     DBNICI010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.144"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(434..976)
                     /locus_tag="AC1ZCC_002324"
     CDS             complement(434..976)
                     /locus_tag="AC1ZCC_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR4 domain-containing protein"
                     /protein_id="HIQ1630550.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
     gene            complement(1289..1840)
                     /locus_tag="AC1ZCC_002325"
     CDS             complement(1289..1840)
                     /locus_tag="AC1ZCC_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIQ1630551.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
     gene            complement(2133..4064)
                     /locus_tag="AC1ZCC_002326"
     CDS             complement(2133..4064)
                     /locus_tag="AC1ZCC_002326"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIQ1630552.1"
                     /translation="METIQEQQSQEKHNHNHNHDHDHDHGKMPVVLYFVGLALAVIAL
                     FLNEDYQLVQNVLFSIATISAGYHVILLEGLGETIENSKAQKKFIPNSHILMGLAAIG
                     ASLMGNFWEGTLLILIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLILPDGSTKNV
                     DVSELKVGDQLQVLNGDQVPIDGVILSGSTSIDESSITGESIPKEKSKGDGVFGSTIN
                     GTGTFTMKVTKENKDTVFSKILQLVNQNQDNQTKAASIIQKFEPKYVTVVLIAIPLFM
                     LLAPFLLDWTWSQSVYRGLVLLVAASPCALAAATVSATLSTTSNLAKKGVLSKGSSYL
                     SQLADIKAIAFDKTGTLTKGKPEVTNYYFADSVNEENMIDIVVALEKESNHPLADAIL
                     RKFEQKNKLTIEVENQIGKGLTGDYKGRNYRIGKPTSFDDVSDEYSRLNNEWASEGKT
                     VVYVAVDEEVIGLIALMDVPSEYAKETIEYFRKQGIHTTLITGDSEMTGKAVAKQLGI
                     DEVIANVMPEDKSRIIDEQKEKYGVTAMVGDGVNDAPALVNADVGIAMGDGTDVAVEV
                     SDLVLMQNNLSKLVQSHNISSKMNRVIWQNIIFSMAVVAFLIVVSFLGLTDIAISVII
                     HEGSTLVVILNGLRLLKAV"
     gene            4363..4677
                     /locus_tag="AC1ZCC_002327"
     CDS             4363..4677
                     /locus_tag="AC1ZCC_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIQ1630553.1"
                     /translation="MDIKTTSPIDKGSILSISKLFKVISDPTRLSVLFLLQKKECSVG
                     NIAISLDMEQSAISHQLKTLKDARLVKSRREGKSMLYSLDDLHVFSILEQVLTHVNEL
                     EK"
     gene            4719..5627
                     /locus_tag="AC1ZCC_002328"
     CDS             4719..5627
                     /locus_tag="AC1ZCC_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIQ1630554.1"
                     /translation="MSKSDKDHNHSNQNQPKKNIKIAFFINLIFSISEFFFGFLFNSV
                     SIMSDAVHDLGDSISIGFAWFFQGYSEKKQDERFTFGYNRFSLLGALITGVVLISGSF
                     FMIYRSVPRLIDPQPVNYSGMFWLSLVAIALNGYAAWILSKGTSKNESMLNLHMLEDV
                     LGWIGVLIVSILMNFTEAYRLDPILSILIALYILYKTVPEFFTTMKILLNGSPENVNV
                     KSLADSIKNVPAVKDLSHFHIWSLDGEENALIITVLIDPSDIDKAREIKEEIAEVAHK
                     SSVENHITIEITTSKEELEKGYSHNT"
     gene            complement(5729..6340)
                     /locus_tag="AC1ZCC_002329"
     CDS             complement(5729..6340)
                     /locus_tag="AC1ZCC_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HIQ1630555.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGIRHIFWGQY
                     LGTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVAFVGEEEEEEEEVVEKLE
                     SRGTNRLFWTVALITIASGGDNLGIYIPYFTSLAFSEIIIALIVFAISVAVLCYISYK
                     LAKISFVSETLEKYERIIVPIMFIALGIFIMIENGTIQTLLGF"
     gene            complement(6357..>6434)
                     /locus_tag="AC1ZCC_002330"
     CDS             complement(6357..>6434)
                     /locus_tag="AC1ZCC_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIQ1630556.1"
                     /translation="SLANEDILSAIRVFLKLSENLSMKS"
BASE COUNT         2020 a         1243 c         1095 g         2076 t
ORIGIN      
        1 ttaagattaa tgacactaaa ttaacagact atacgtagta tctatttgct atgaccatat
       61 cttatatcca taagtcaact cagccggact aaaagggcta atcttactag aacacaagct
      121 gcacttgata tgcaaattaa ccctgaatcg gaaaggtgct taaattggat attaacggag
      181 ggataaagta aaatttatct ctagggaatg tttagaaagg atccaagttc cttaacaagt
      241 gacaattagt aatgtagtaa caacgatagg ttgtatgccg aacatttcaa tttgatacgt
      301 ataaaagacg atttcaagct catccgtgat aaataaccca catgaatgat ttgaaatcgt
      361 cttaaaatgc tttattttat aacccaaaat agaataatta cttcaaaatg acgtcgcata
      421 tcactattaa tgattaatcg atgacttcca taatcatttt aagttccccg gatgttaaat
      481 cctcgctaaa aatgccagta actttttgtc caggtccaat ttcctttgat gataggttta
      541 acaaagagat agggatatag attaatccgt tttgtttcaa agacagagcc aatatttttt
      601 tccttgttcg aatagctgca tcatagcgaa gagaagagta aacacctctg ccaacaatag
      661 aaatatgctt ccccaataat aaatcaaggc tacctattac ttgtaatttc tgaaaggtcg
      721 tcccatccgt tttgatttga atatcctgta aatttatttt cctgtctaag gcatcttgaa
      781 aaaacaagtg tgtgcctctg cgataatcaa tgccacagag gtgcataaaa ttagttgtcg
      841 aaaaaataat cgctaccgtt gagacttctc cgacaaagga tactttttta tctacaaaat
      901 gttcttgtaa gagatgagca gccttataaa tcgcgtttaa ctgtctggta atttgggttg
      961 tctcttttat agacatgcgt ttgtattctt tttttgccgc gtgcgaagaa tgctcctttt
     1021 tcccaataaa aaaagctgct caaaagagca gccttttgta caggtttagt tttggtgctt
     1081 gccacccgcc ggacccttac gttccggatt tgtatcgagt ttagttttgg tgctcgccac
     1141 ccgccggacc cttatgttcc ggatttatat caagttgact atgagcctgc tctcatttgt
     1201 atatataata taccaaaaaa aggttgattt gtccaatact gtgggtgatt ataaccgtct
     1261 tactgtgctt atctattata taatacaatc aaaaacctaa ccgaaaattt aataacgttt
     1321 cactgatttc atcttcatta attccgatgt agcgtttggt aatcttctcg ctgctgtgac
     1381 caaagatttc cattaaggtg gccacgtctt tggttttctt gtaatagtgg taaccaaagg
     1441 tttttcgtag ggtgtgggtg ccgatatcgt cccttcctaa cagcttcgcg accttttgaa
     1501 acatctggta gaccgtattg acttctaaat ggccgccctt agtactggga aacaagtaag
     1561 cctccggatc taagggtgcg gtatagccgt gaatcaagtc ctgtaagctg cttaagtata
     1621 aaatgcgtgt tttccctgtc ttttgctcca cgatgcgcgg gttttttgag gtaatcacgt
     1681 cttttttctt gagtttaacg atgtcggaca tgcgcagacc gctgttaatt ccaattagaa
     1741 acaagaaaac gtcacgttcc gcatttttat tccgtcgcag gcaaaataaa aaatcattaa
     1801 tctcctgttg cgaccgtagt ggctgaacat tatagcccat gacaatccct cctttttagt
     1861 ctgttcgaat acatgattat agttattaca acacgaaatc gtgtgtttag ggggagtaaa
     1921 aaaaaagaac cctataagga taggattctt gatacatgat tttatccaat ttcatgtatc
     1981 ttatattcaa cccgcgccac aagagtatgg ttgatatatc attttgactt tgaacatcta
     2041 gataaatata aacaagtaat agagataaaa aatgtagtgt aatccttaat tatctctatt
     2101 actcgattaa tttgaagttg tgcaatcatt ctttagactg cctttagcaa tcgaagtcca
     2161 ttcaaaataa caaccaaagt acttccttca tgaataatga cactgattgc aatatccgtt
     2221 aagcctaaga aactaacgac aattaaaaaa gcaacaacgg ccattgaaaa aataatattc
     2281 tgccaaataa cacggttcat tttcgaagaa atattatgag attgtaccaa tttagataaa
     2341 ttgttttgca ttaaaactaa atcagatacc tctactgcca catcagtccc atctcccata
     2401 gcgattccca catctgcatt aacaagagca ggagcatcat ttacaccgtc tccaaccatg
     2461 gcggtcacgc cgtatttttc tttttgttcg tctataattc tggatttgtc ttccggcatg
     2521 acattcgcaa tcacttcatc tattcctaat tgtttagcaa ccgcttttcc cgtcatttct
     2581 gagtcaccag taattaatgt ggtgtgtata ccttgtttcc taaaatattc aatggtttct
     2641 ttagcatatt cactcggaac gtccataagg gcaataaggc ctataacctc ttcatctact
     2701 gctacgtata cgactgtctt accttctgac gcccattcat tatttaaacg actatattca
     2761 tctgaaacat catcaaacga agtcggtttt cctatacgat aatttctccc tttgtaatct
     2821 cccgtcaatc ctttaccaat ttggttttct acttcaatag ttagtttgtt tttttgctca
     2881 aactttctta gaatagcatc tgctaagggg tgattggatt ctttttcaag agctactacg
     2941 atatcaatca tgttttcttc gttcacggaa tcagcaaaat aataattggt tacttcaggt
     3001 tttcctttgg tcaaggtgcc cgtcttatca aatgcaattg ctttaatatc ggctaattga
     3061 gacaggtaag aactaccttt tgaaaggact ccttttttgg ctaaattaga agtcgtcgat
     3121 agcgttgccg agacagtagc tgctgctaaa gcacacggtg aagctgcaac taaaaggacc
     3181 aatcctctat agacactttg tgaccatgtc caatcaagta gaaaaggagc caataacatg
     3241 aatagaggaa tggcaattaa aacaactgtg acatatttcg gttcaaattt ttggataata
     3301 ctcgcagctt ttgtttggtt atcttggttc tgattcacta attgtaaaat ttttgaaaat
     3361 actgtatctt tgttttcttt ggtgactttc atcgtgaagg tacctgttcc attgattgta
     3421 ctcccgaaga cgccatctcc tttggatttc tctttcggga tactttctcc agtaatagac
     3481 gactcatcaa tggatgtaga tccagacaaa atgacgccat caataggtac ttgatcgcca
     3541 ttcaacactt gaagttgatc tcccactttt aattcactga catccacatt ttttgtacta
     3601 ccatcaggca ggattaaccg ggcagtcgtt ggattcattt cgagtaattt cgttatttct
     3661 cgtttgcttc ttccttctgc ataatcttcc aaaaaatgtg cgccagaaaa aataagaatc
     3721 aataatgttc cttcccaaaa attccccatc aaagaagctc caattgccgc taatcccatt
     3781 aaaatatgag aattaggtat gaactttttt tgtgcttttg aattctcaat tgtttctcca
     3841 aggccttcaa gaagaataac gtgatacccg gcactgattg tagcgattga gaacaaaaca
     3901 ttttgtacca actgataatc ttcattcaaa aatagagcaa tcactgctaa tgctaaacca
     3961 acaaagtaca aaacgactgg cattttccca tgatcatggt cgtgatcgtg gttgtgatta
     4021 tgattatgct tttcttgaga ctgttgttct tggatagttt ccacttctat tgcctcccta
     4081 tataatttat aattcctttg ttttccatca tttacttctt gaatcacata aatttctgct
     4141 atttctgact cctttcgttt attccaaaag taaatcgtaa tgaatccacc ttacatatga
     4201 ctaattaatc atatgtttgt atactcattt tatatcgcta tatacatttt gtcaagataa
     4261 agagaacgcc acttctcttt tataaacaaa agattagcat tgtttcgctc atatggtaat
     4321 atagatatga acggaaggtc atatgaaagg aataaaaaca ctatggatat aaaaacaact
     4381 tctccaatcg ataaaggatc tattctgtca ataagcaaac tatttaaggt gattagtgat
     4441 cctacacgat tgtcggttct ctttctttta cagaaaaaag agtgtagtgt tggaaatatc
     4501 gctatttctc tggacatgga acagtccgct atttctcatc aattaaaaac gttgaaagac
     4561 gcacgcttag tgaaatcaag aagagaagga aaaagtatgc tttacagttt agatgacctt
     4621 catgttttta gtattctcga acaagtctta actcatgtta atgaattaga aaaataaaac
     4681 caattagtat gtagcataca aacttaggag gtttttaaat gtcaaaatca gataaagacc
     4741 ataatcactc gaatcagaat caaccgaaaa agaatattaa aattgctttc ttcataaatc
     4801 ttattttctc tataagcgaa tttttctttg gctttctttt caatagtgtg tccattatgt
     4861 ccgacgcagt tcatgattta ggggactcta tttcaattgg attcgcttgg ttttttcaag
     4921 gttattcaga gaaaaaacaa gatgaacggt tcacttttgg ctataaccgc ttttctttac
     4981 tgggcgcgct gataactggc gttgtgctaa ttagtggctc gttttttatg atctatcgta
     5041 gtgtgccacg tttgattgac ccacaacccg taaattatag tggcatgttc tggctttctc
     5101 tcgtagctat tgcattgaat ggatatgctg cgtggatttt aagcaaaggc acatccaaaa
     5161 acgagagcat gttgaacctc catatgctgg aagacgtatt agggtggatt ggagtcttga
     5221 ttgtgagtat tctaatgaac tttaccgagg catatcgtct ggatcctatc ttatcgattt
     5281 tgattgcact ttatatcctc tacaaaaccg ttcctgaatt tttcaccaca atgaaaattt
     5341 tattgaatgg ttcgcctgaa aatgtaaacg taaagagctt ggcggactcc attaaaaatg
     5401 ttcctgctgt aaaagacctc tcacattttc acatttggtc actggacggg gaagaaaacg
     5461 cccttatcat cacggttctt atagaccctt cagacataga caaggctagg gaaataaaag
     5521 aagaaattgc agaagtcgca cacaaatcaa gcgtagagaa tcatatcaca atagaaataa
     5581 ctacaagcaa agaagagctt gaaaagggat actcccacaa cacgtaagat agttctacgc
     5641 taaaaaatac ttcattattt gaaaagcaaa aaaggcagcg attttgatta gtcgctgcct
     5701 ctctttttat ctttcgtgat tatgattttt aaaaacctag tagcgtttgt atcgtcccgt
     5761 tctctatcat aataaaaatt cctaacgcaa taaacattat agggacaatg attcgttcgt
     5821 atttttctaa cgtttcggaa acaaaagaaa tcttggctaa tttgtaactg atatagcaga
     5881 gaaccgcaac agagatagcg aatacgatta aagcaataat gatttctgaa aaagctaacg
     5941 aggtaaagta tggaatataa atacctaaat tatcgcctcc agaagcaatc gttattaagg
     6001 caactgtcca gaataagcga ttagttcctc tagattcgag tttttcaacg acttcctctt
     6061 cttcttcctc ctcttcgcca acaaaagcga ctctgattcc taagaaaatc ggaattagac
     6121 cgagaagacc aatgatccaa tcttgcggaa taaaatttag cacataagca gcaaacaaac
     6181 ttacggctac caaaatacct gttccaagat attgacccca aaaaatgtga cgaatacctt
     6241 tttttgtgtg gctttgagaa aagataatca gcaagataaa caaataatca atactggtag
     6301 aaatataaac agcgatagct gaaaaaatac tttgtaacaa aataacttcc tccaaactaa
     6361 gatttcatag atagattttc tgataacttt aaaaaaacac gaatagcgga aaggatgtct
     6421 tcattcgcta aaga
//
LOCUS       DBNICI010000144         6401 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.145, whole genome shotgun sequence.
ACCESSION   DBNICI010000144 DBNICI010000000
VERSION     DBNICI010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.145"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            330..1175
                     /locus_tag="AC1ZCC_002331"
     CDS             330..1175
                     /locus_tag="AC1ZCC_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIQ1630557.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="AC1ZCC_002332"
     CDS             1456..2676
                     /locus_tag="AC1ZCC_002332"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIQ1630558.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="AC1ZCC_002333"
     CDS             2673..5393
                     /locus_tag="AC1ZCC_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIQ1630559.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="AC1ZCC_002334"
     CDS             5377..6321
                     /locus_tag="AC1ZCC_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HIQ1630560.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2380 a          989 c         1233 g         1799 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattggctct t
//
LOCUS       DBNICI010000145         6365 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.146, whole genome shotgun sequence.
ACCESSION   DBNICI010000145 DBNICI010000000
VERSION     DBNICI010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.146"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(26..1006)
                     /gene="pta"
                     /locus_tag="AC1ZCC_002335"
     CDS             complement(26..1006)
                     /gene="pta"
                     /locus_tag="AC1ZCC_002335"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HIQ1630561.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(1159..1839)
                     /locus_tag="AC1ZCC_002336"
     CDS             complement(1159..1839)
                     /locus_tag="AC1ZCC_002336"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIQ1630562.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            1965..2834
                     /locus_tag="AC1ZCC_002337"
     CDS             1965..2834
                     /locus_tag="AC1ZCC_002337"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIQ1630563.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            2860..3375
                     /locus_tag="AC1ZCC_002338"
     CDS             2860..3375
                     /locus_tag="AC1ZCC_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HIQ1630564.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(3422..3985)
                     /locus_tag="AC1ZCC_002339"
     CDS             complement(3422..3985)
                     /locus_tag="AC1ZCC_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1630565.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(4040..4219)
                     /locus_tag="AC1ZCC_002340"
     CDS             complement(4040..4219)
                     /locus_tag="AC1ZCC_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630566.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(4296..4805)
                     /locus_tag="AC1ZCC_002341"
     CDS             complement(4296..4805)
                     /locus_tag="AC1ZCC_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630567.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(4901..5280)
                     /gene="gloA2"
                     /locus_tag="AC1ZCC_002342"
                     /pseudo
     CDS             complement(4901..5280)
                     /gene="gloA2"
                     /locus_tag="AC1ZCC_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644319.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SMU1112c/YaeR family gloxylase I-like
                     metalloprotein"
     gene            5610..6359
                     /gene="pnuC"
                     /locus_tag="AC1ZCC_002343"
     CDS             5610..6359
                     /gene="pnuC"
                     /locus_tag="AC1ZCC_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HIQ1630568.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         1832 a         1266 c         1166 g         2101 t
ORIGIN      
        1 tttttggcta gttcgcgtgt atttgttaat caatcaatgt ttgtgctgct gtgataatcg
       61 ataatttata tacatcttct tcatttgctc cacgagacaa atcagaaacc ggtttgttca
      121 agccttgtag aattggtccg attgcttcaa agttaccgaa acgctgagca attttatagc
      181 cgatatttcc tgactgcaat tctggaaata caaagactgt tgcttgacca gctacttctg
      241 aatctggcgc tttcagttgt gcaactgaag gaacataaga agcatcaaat tgcaattcgc
      301 cgtcgatcat gtattgtggc gctagttttt tagcgatttt agttgcttca actactttgt
      361 ctacttctgg cgatttcgct gagccttttg tcgagaagct catcattgct acttttggtt
      421 cgatatcgaa caattctgct gtttttgcag aatctacggc gatttcagcc aattcttgcg
      481 cagttggatt cacgttgatc gcacaatccg aaaagacata tttttcttga tctcttccac
      541 gtaccatgat catcgctccg cttgtccgac tgatgccagg ctttgttttg atgatctgaa
      601 gtgctgggcg aacagtgtcg ccagtcgaat ggatcgctcc agagaccatt ccgtctgcaa
      661 tccccatata cgtaagcatc gtaccaaagt aattcacatc ttttaaaatc ttttctgctt
      721 gttctttgga tgcctttcca tttctacgtt caacaaaagt atccaccatt tcatcccact
      781 tatcgtaatt atcagggctg atgatcgtaa agctagatgt tttgattcct ctggcatgcg
      841 ctgcatttct tatttcttct tcgttcccta ctaaaatggg ttccacaagt tcttctgctt
      901 tcaaccgggc agctgcgcct aaaatacgag catcagtagc ttccgggaaa acgattttga
      961 tcccacggcg aacaatttta aatcttaaac tatcaaacaa ttccacaaga tgacctccag
     1021 acgtttttat atgtattatc atacactatt ccagaaaaaa agaacagttt attttatctg
     1081 tatcagtcaa aaatgctgga aaaattaagt ttttcacata caaagcgaac tgcactataa
     1141 caggaaagcg ctgttttatt acactgtttc aggcagttgc caatcaatcg gttcttctcc
     1201 taacgcaagg agcgcatcat tcgttttcga gaagggtttg gaaccaaaaa agccacgact
     1261 tgcagataac ggactgggat gtggagatgt tatgatgata tggcgactct tatcgatcat
     1321 tttgattttt tcttgtgccg gttttcccca caagataaac acgattggtt tttcccgttc
     1381 atttaatttt tcaatgatcg tgtctgttaa tctttcccaa cctttcccgc gatgagaata
     1441 cgcttgccct tcccgaacgg taagaacggt gttgagtaaa aggactccct gtttagccca
     1501 tgacactaga tttccatgtt gaacaggtgc gattccaaga tcatcataaa gctctttata
     1561 gatatttctt aacgatggcg ggatctttac gccaggttgc acagaaaaag ataagccatg
     1621 agcttgattt tctccatgat aaggatcttg tcctaaaatc accactttaa cttcttcata
     1681 aggagtcaat tccaatgctt catagatatg atacatatct ggatggatct tttgggacgc
     1741 atattctttt tttaaaaatt ctcttagatg cagataataa tcttttgaaa attctgagga
     1801 caaaacttct tgccaactgt tatgaatgat ttctttcaaa aactccacct cctaataaaa
     1861 agaataactc acattttctt caatcacaag ggacaatctt caccctcagc acttgattaa
     1921 tatggtaaac taggaacaag aagcagaaag gaacgataat cattatgatc aaattaattg
     1981 catcagatat ggacggcaca ttattagatg cccatatgag tgtttctcct gaaaatgccg
     2041 aggcgatacg ttttgcaaat gatgccggtg ttgagttcat ggtcgctact ggtcgaaact
     2101 atcaagaagc tcgcgcagct ttagatgagg taggaatcga ttgtgcgatg atcactttga
     2161 acggggcaca ggtatttgat aaagaaggaa attcattgtt taccgtgtcg attccaaata
     2221 atcaagcaat ctctgtttta gatattctag atgctaacgg tatttactat gaagtagcaa
     2281 ccaacgatgg tctttattct gaaaatcaag cgaaacgaat tgaaagtttt gcttccatgg
     2341 tggctaccca tttacctcac cttacgtata aaatggcgat tgccatggcc tcagctaagc
     2401 tggagttatt gcacatcaca tacgtggaca gtattcgtgc tattttggaa gaaaagaatc
     2461 tggaagtttt aaaaattatt tgttttcata cagaagggcc agctgtatta ggtccagttg
     2521 gaaagcagat ttctctttta ggcgatctag ccgttacttc ctctggtcaa aacaatatcg
     2581 aagtgaacca taaaaacgct caaaaaggaa ttgctgttgc ccatgtcgct cacgagcgtg
     2641 ggatttcttt agaagaagtc atgacgattg gcgataactt caatgatgtc agcatgctgc
     2701 aagtagcagg agtcagtttc gcaatgggaa atgcagaaat cgaagtaaaa gattatgcca
     2761 aatatacaac ggacacaaac cttgaatctg gcgtaggaaa agccatattg cgcgcgattg
     2821 cagaagactt gtagttttat ctagaaaaga gggatataga tgtcagaatt tttcattcag
     2881 gaaaagcagc ttggacgtgc aactcggaca attgtcaaaa acgaaaaagg acattccctg
     2941 tttttgatgg ttggtcgctg gggaacaaag ggcgatgcat tgtctcttta tgccatgaat
     3001 ggcgatctcg ttgctcacat caagcagata agcctgacat tcggtacgcg ttttgagctg
     3061 tataaagagt tcgaaaaggt gggaaccatg cagaaaatat tcaattggcc cggtgacttt
     3121 tattatattc gtagacttca ctggacagtc caaggtgata tctataatca tcaatatcaa
     3181 atccatcatt tcaatcagca aatcatgaaa atggataaag caacactttt ttcaggagat
     3241 tactatgtat tggatgtctc aaatgatgaa gatgctccag tttgtatttg tattgctgcg
     3301 gttttagatt actggctcta ccgtaaagac aaaagtcgcg ataagcctgt ttcttaccga
     3361 cttaacttaa gttaaaaaga tgtacctgaa atgcggaaat acgcatttca ggtacatctt
     3421 tttagatatt cttttgcata tgatcataaa gatgaccact gatcatccgc tgaccgacaa
     3481 ctttgaagtc atggcgttcg tataattttt tagcctttgg atttttttca tccacactta
     3541 gaccgatgac ggagcgatta gctttcttcg ccaatttagg taatgcttct aataatcgcg
     3601 aaccgatgcc ttgtcctcta aaatcttctg atacactgat agagtccaaa taccattcgt
     3661 tgggaaacgt ttctttatca atgaacaaac gtgtagacgc atctagctga tatttctcta
     3721 agataggtac cagtggctga tcgatgattg attcttcttc atctgtataa ccgtaagcaa
     3781 ctcctgcaac tttaccgtct atttcttcta cgatacctcg tgaataactg taacgataat
     3841 ttggatcagt gattgcttct tctaaaatct ctaaagtctt ttgttcccct gtctgcttca
     3901 aaaatggcag ttccatatct tttagaatca ctaagatcaa tggggcgatt tctggagcat
     3961 cttcttttct cgcaaaacga atcatagttt cctctccttt tctccattct aacatgcaga
     4021 aaaagaaatc gcaaaatcgt tattttaaaa aatcttcaat cttttcagta atgaaataac
     4081 catgagtaac gattcttgcc gcttcagttc ggcaataagg gcagacgact ggcatttctt
     4141 tatcattatg ttcgactaca ttattgactt cttcgtatgc aaattttttt ccgcaattag
     4201 ggcatttcat tttttccatg ttatctcaac ttcctttcat ctttactctc ttagcttact
     4261 cttatattgt aaaaaagaga agcaatatcc ttcttctatg cagatatcca tacctgacgg
     4321 tcctgttctc catctattaa tcggacttta taataaggac gttcatcttt gactagaagt
     4381 gtccactctt tcacttttgc ttttttcccg tagtagctga ctgcttcctt catcgctgct
     4441 tgcggttgtt tgaactcttc cgttttttct atatcaaaaa gatgttctgc ggccttttcc
     4501 gtgtcatttc gtacggttgt tttcccagtg gatggattga ctgctatgac ttgtccttca
     4561 caaaaaaact ggtattcata tgttgcattc gtaggattct ccaaatcttt ttcaactgca
     4621 aaatgaatag cagatatgtt atgttctttt gtttcgtttt ttacgattgc tgctgctttt
     4681 ttcaatgaga tccgatattc ctggtcacta atatcatcca tgtgaaaagc ggcattgagc
     4741 agtcccgtca taccggtaat ccctattacg atgagggcag caacaccaaa catcattttt
     4801 ttcattcttc attctcctgt ttcttttttt taaagaatat caagaaatga acgaatgaaa
     4861 aatcgaatga aacgaaaaaa aagtgacaat tttgttatct ttattcgtgc agttctaatg
     4921 gcaatccgtc tggatcaaag aaaaacgtca tttttttcct gtaaaggtat cttctctaat
     4981 cggttctgat tcgatcccta aggactgcaa ttctgcaatt gtttcttcaa tatcgtctac
     5041 tttgaaagca agatgacgca agccacaagc ttctggatag tttggtcgtt gtggcgcatt
     5101 agcaattcca aaaatctcca attcagcact tcctagtttc agatctaatt tatagtcatc
     5161 tcgttctttg cggtaatttt ctctgattac ttcaaatcct aatttattaa cataaaaatc
     5221 acgtgatttc tgataatctg aaacaatgat ggctatatga tggatcgttg taaggttcat
     5281 atgagcactc ctattcttcg tttaagctcg gatctatatt aatctccagt tccattgtaa
     5341 atgtgtcaga ttgaaaaaaa tagctgtttt tcctttttat aagaaaaaag gcaaaaaaaa
     5401 cagaaagtag gtcctccaag acactcttcc tgttcccaat ataataaatg aaagtaagaa
     5461 attatatttt caagaaaaca aacagaaaat tatcataaca caaattattt ataattgaaa
     5521 gactagtttt ttttcaagaa aagcatacaa tagattgtgt tagtaaatac agaacacact
     5581 acatttagtg tcttaaggag tcctcataca tgaagtcata ttttcagttt ttagctcatc
     5641 agctaaaagg ctggccgcaa caaaattatt atcttttctt tttcagtctt ggctgccagg
     5701 tcatgactct tgtcaatagt cccatcactt ggctgtccgt tattacattt atcggtacca
     5761 cgcttggtgt attgtgtatt ttatccatca atgcggcaaa atcagtcaac ggtattttag
     5821 gtattttgtc tgcgatttgt tttattatcg ttgggttttc agcaaagaac tatttaagta
     5881 tcggtgaaca gattgcttat atgatcacat tggatatccc cgtattgtta agtaaagaat
     5941 ggaatcataa tatggcttca aaaatccgaa aattcactgg aaaaagctgg tttatcgcaa
     6001 tcatcagtac tttccttgtc tatcttgttt caggattcct gattggtcgt tatacagatg
     6061 atcctcggcc gtgggtagat gccattgctt tttctattag cttgactgct ggtatcatca
     6121 gtttcttgcg ttacaacaac caatattact ggtggttagc ttcagggctt gctcaaatgg
     6181 tcctttggta tatttcattt acgcatggat ctgcaagttt ggcgatgttc gtgaacagct
     6241 ctgtttactt gctgaatgat gtccttgcat ttaccgtttc accttggtat aaccaaaaag
     6301 aacgtgaacg tatgcaaaaa atggaagaag aatactacaa cgcacaacaa cacgcataaa
     6361 aaaac
//
LOCUS       DBNICI010000146         6218 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.147, whole genome shotgun sequence.
ACCESSION   DBNICI010000146 DBNICI010000000
VERSION     DBNICI010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.147"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            133..2922
                     /locus_tag="AC1ZCC_002344"
     CDS             133..2922
                     /locus_tag="AC1ZCC_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HIQ1630569.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            2969..3496
                     /locus_tag="AC1ZCC_002345"
     CDS             2969..3496
                     /locus_tag="AC1ZCC_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HIQ1630570.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            3517..4707
                     /locus_tag="AC1ZCC_002346"
     CDS             3517..4707
                     /locus_tag="AC1ZCC_002346"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIQ1630571.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            4747..6045
                     /gene="asnS"
                     /locus_tag="AC1ZCC_002347"
     CDS             4747..6045
                     /gene="asnS"
                     /locus_tag="AC1ZCC_002347"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HIQ1630572.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
BASE COUNT         2060 a         1153 c         1363 g         1642 t
ORIGIN      
        1 aaaataaacg cccagacatt tgcgtgtctg ggcgtttttg tctattttct ttaaaaagcg
       61 atgagagtat ttttctgcac tttgtagtat aatttcaaaa gaacattaga ggcaggagaa
      121 gaggaagata ttttgaaacc aagaacttat gcagtcgttg atatcgaaac gactgggacg
      181 aatccaaaag aagatcggat catccaattt ggctgcgtca tgattgaatc aggtcggatc
      241 acttcacgtt tttcgatcga tattcatcca gggcggaaga tttcaaaaca aatccagcat
      301 ttaacaggga tcacgaatca acgcgtccaa aaagcgcctt atttcgaaga tgtcgcccaa
      361 acgatttaca atcttttggc agacaccata tttgtcgcgc ataatattta ttttgattat
      421 aattttttga accatgaact catgcgatgc ggacttccta gtttgaagat accaggcatc
      481 gatacagtag aacttgcaca aattttcttg ccgacggaac caagctttcg cttagctgat
      541 ttgtcagaaa gccttggatt tattcatgag aatcctcatc aggcagatag tgatgccgaa
      601 gtgacaggtc aacttctttt gctgatcgaa gaacggatga gaaaattgcc aatcatcacg
      661 ttagagcaga ttgcgcgatt aagtagacac acaggtatgg atacgagtcg tttcatttat
      721 catgtgattg aagaaatgaa agaaaaacca gagccacttg atccctcatt agaaatcgtc
      781 gatgggctag ctttgcagaa aaaagaagtg gaactattca catccgtcca ttatggcgag
      841 cggacatatc caagaaaaaa acaagcaaaa gaaaaaatgt ttggtaaaac actgatgtat
      901 cgaaaagaac agaacagact gatgaatgcg gtctacgacc atttcacaaa agatgaatca
      961 aaagatttga tgatcgaagc agctacagga atgggtaaaa caatcggtta tctgctgcca
     1021 ttgagttatt tggctacacc tgaaaaaccc gctgtcatca gtaccgtttc gctagttttg
     1081 cagcagcaga tcatagaaaa agacattcca ttgctgaatc aattgctaga tcagcccata
     1141 caacctgtaa ttatcaaaag ctatcgccac tatattgatt tgcagcgttt taaaggcaca
     1201 cttgtggaac cgccagaaca aaaacaatat gctttgtatc aaatggcggt cttagtttgg
     1261 ctgacgcaaa cgaagactgg tgatttggat gaattacatt taacaaattt gaaccattca
     1321 ttctttgcag atatcgcgca cagaggtatt ggatttttag caagaaatca gtcattttac
     1381 gaacaagact ttgttcgttt cttgcaaaag aaaatcaggc aaagtaattt tttgattgtc
     1441 aatcatgcgt ttttagtaca agaaacacag cgaaaagaac cattgcttcc agctagtcca
     1501 tttttgttga tcgatgaagt acatcatttg ccggaaatcg ctgaaaaagt aaatgacaaa
     1561 cgattaagta ttaactattt ccataaacaa atccagcaat tagaagaacc aaatcagctg
     1621 tttgaccgaa tacaaagcta tttaccgaaa gaacatgaag ccttacgctg gttatcgatt
     1681 tatcgagaag aactttctgc gctatgcgaa gcacagattt gtatggcaga aggattaatg
     1741 agtttagtga gcggcaagca acctaacgga cagtatccag aagaagtcat gatgacaaaa
     1801 gagatgttgg atcagctgcc actggaatct gggacagcca ttgatcacct gcagcttttt
     1861 tatcaggaga tccagcagct acagtcacaa atgcgtctga cttttgagat tatccaagaa
     1921 agctggacaa accagatgcg gatcgattta gcaagtttat atagcctatt tgatcaatta
     1981 gacaagcaat acttgctgat ggatcaatgg ctggaagact ggcgagatca tttgatccat
     2041 tggttcgttc ctgggaacac agcgcagcag atgatttttc atctccatga ttttcaagcg
     2101 gctatgttgc cgtcgactgt ttggtatcct cgatatgatc gtattcttta tattggagga
     2161 acgctgaaga tcggccctaa tcgacactat ctctctaaga ggcttggcat tgaagcagca
     2221 cctcttaaag tgattgcctc tccttatgat tatgagaaac aggcaaggat ctatgtgcca
     2281 aaagaggcac ctgccgtcca tcaagtcagc aatcaagcat atatcgatta tttagagaca
     2341 gcacttacag gtctgatcga acaagaagat cggccaatac ttgtgttatt cacttctcat
     2401 gatgttttgc agaaggtcta tcagaaaatg catctgtcta tgctagaaaa aggacgagaa
     2461 atactagctc aagggtttgg tggaagccgg gagaagcttt tgaaacgctt cattttgtca
     2521 gaaaaaagcg tattgtttgg cgctgacagc ttttgggaag gtgtagattt gcctggagat
     2581 gctttgcaga tcgttgtagt cacacgttta cccttcgata atccgcttcg cccgatggtc
     2641 aaagctagga acgcttattt agaatcagag gggatcaatc ctttttacca ggaatccgta
     2701 cctaaagcag ctttgaagtt acgccaagca ttaggtcgct tgataaggac ggagacagat
     2761 cgtggcgtat tgcttatttt ggatcgaaga ctagtcactg caaaatatgg cgcacggatc
     2821 atccatgcat taccgaaaaa agtaccgatc aaagagctag ctatggagga aatctatcag
     2881 gatatccatg aattcttaaa gaaaaatgaa gaaggaagtt agaggagctg tcttttttca
     2941 taattaaaat ctgctataat gaaaaggaat gaagataaag gagcgacgac ccttgaagaa
     3001 aaaacagaca aaggaaaaaa cgattaatcg cgtgttactt ggcttgatcg tatttttatt
     3061 agcggtcatc gtgatcagct cgatttttta cttgcgttcc agtcgtccgg cggtgcaggc
     3121 gaagaaagaa gcagtagaaa tcgcaaagaa atatgcaaat cttgaggaaa ccgatcattt
     3181 ttattggttt actcgaaaga agacatactt ttcactgaca ggtacaaatg ataaaggaaa
     3241 agaaatcgcc gttatcattc ctaaatctgg tgaaaaagta aaagtattgg atcaaacaaa
     3301 gggactgact gaaaacgaag caaaacagac aattgctgcg gcccatcctg aaatccaaat
     3361 cgaaaaagct gctttaggga tgtatgatga tcagcctgtg tgggaagtag taggaaaaga
     3421 aacaaatggg aaactgaatt attacctgat tggctttgaa tctggagatg aagtcaagac
     3481 aatcaaggga atttaaaagg taaaggaatg aatgggatgg agttatcaaa aagaacaaaa
     3541 cgattagaac cgtctgtcac tttggcagct gcggcaaagg ccaaggcatt aaaagcaaaa
     3601 ggcagagatg tgctgagttt gactgtggga gaaccggatt ttgcgacacc agaaaacatc
     3661 caagaagcag caatcgaagc aatccgcaat gggaaagcta gctattatac accaacagca
     3721 ggaatcccag agcttcgaca ggcaatcgtt gattatctga agaaaaacta tggacttgtc
     3781 tatgaacctt cacaaacagt cgtaacagac ggagcgaaat ttgctttgta tacccttttc
     3841 caaacgatcc ttgacccgca agatgaagtc atcattcctg tgccttactg ggtcagttac
     3901 ggtgagcaag taaaactggc agaaggactt cctgtctttg tgaaagggga agaatccaat
     3961 agctggaaag ttactgttga acagctagaa caagcacgtt cagaaaaaac aaaagccgta
     4021 atcatcaatt ctccatcgaa tccgactgga atgatttata gcaagaatga actgcaggca
     4081 atcggtgaat gggcagtaaa aaatgatatt ttgatagttg ctgatgatat ttatggccgt
     4141 ttagtttata atggcaatga gtttactcca attgcaacga tttcagaagc aatcaaaaat
     4201 cagaccatca tcatcaacgg cgtgtctaaa acatatgcga tgacaggctg gcgcattggt
     4261 tatgcggtgg gaaatcctgt gatcatcaat ggaatgatcg ctgttgcttc ccaatcaact
     4321 agcaatccga cagcagtcag tcaatatgca gctgttgaag cattgaaagg tgagcaagat
     4381 acagtagaag aaatgcggaa cgcctttgaa gaacgcctga atacgttata tccgcttgtg
     4441 gcagaactgc cgggtgtttc tcttgaaaag ccgcaaggag ctttctatct ttttcctaat
     4501 gtaaaagaaa cgctacgcat gtgtaagtat gaaaatgtca cacaatgggt agaagatctt
     4561 ttagaagaaa caggggtagc gcttgtgact ggcgaaggct ttggggcacc agaaaatgtc
     4621 cgcatgagtt atgccactga tcgcgctacg ttagaagaag cggtacgcag aatcaagcaa
     4681 tttattgaga gtaaaagtca aaattaatcc tgaagaaatt caaggaatat aaaaggagag
     4741 tcatacgtgg aacaaattca aatcatcgat tcgaaaaacc atgtgggaga aacagtaaca
     4801 atcggcgctt gggtcgcaaa taagcgatca agcggaaaaa tcgcattttt acaattaaga
     4861 gacggaacag cctttttcca aggtgtagtt gtaaaaagcg aagtgtcgga agaagttttc
     4921 caaacagcta aaaatctaca ccaagaaaca tccgtgttag taactggaga aatccgtgaa
     4981 gacagtcgtt caaaatttgg ttatgaaatc ggaattacaa atgtggaagt ggtaagcgag
     5041 agccacgatt atccgatcac accaaaagaa cacggaacag atttcttgat ggatcatcgt
     5101 catttatggc tgcgttcttc aagacaacat gcgattatgc agatcagaaa tgaaatcatt
     5161 cgtgcaacgt atgaattctt taatcaacat cattttgtca aaatcgatcc gccgatcttg
     5221 actggttctg caccagaagg cactacggat ctattcgaaa caaattactt tgatcaaaaa
     5281 gcttatttat ctcaaagtgg ccaattatac atggaagctg ctgctatggc tttcggaaaa
     5341 gtcttttcat tcggtccgac tttccgtgct gaaaaatcaa aaacacgacg tcatttgatc
     5401 gaattctgga tgatcgaacc agaaatggct ttcactcatc aagaagaaag tttagaagta
     5461 caggaacaat atgtagcatt cttagtacaa aatgttttag atcattgcga atatgcatta
     5521 gatgtactag gacgagacaa agaagtctta aaaacttata caaaattgcc attccctcgt
     5581 attacttacg atgatgcaat cgaattactg aagaaaaatg gatttgatga catcgaatgg
     5641 ggcgaagact ttggttcacc tcatgaaacg ttcattgcga attcattcga acagcctgtc
     5701 tttatcctaa actatccaaa agccatcaag ccattctaca tgaaaccaca tccaacaaga
     5761 gaagacgtgg taatttgtgc agatatgatc gcaccagaag gttacggtga aattattggc
     5821 ggaagcgaac gggcaacaga ctatgatttc ttgctagaac aaatccgtaa tcacggtttg
     5881 gatgaaaaag aatattcatg gtatcttgat ctacgtaaat acggttctgt tccgcactct
     5941 ggttttggtc taggccttga acgaacagtc acttggattg ctggtatcga ccatgtacgt
     6001 gaagctattc cattcccacg tttgttgaac cgaatttatc cataagaggt gatgaaaaag
     6061 ctgttctgca tcaagtaatt agaacagcca agcaaaaaca gctgttcatg tttttgaatt
     6121 ggcgggactt attaccgcag atgtaagctt ttgaataccg tttagtggag aggttgtgaa
     6181 aaagctgttt agtttcgagt attaagaaaa attttgtg
//
LOCUS       DBNICI010000147         6127 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.149, whole genome shotgun sequence.
ACCESSION   DBNICI010000147 DBNICI010000000
VERSION     DBNICI010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.149"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(70..1212)
                     /locus_tag="AC1ZCC_002348"
     CDS             complement(70..1212)
                     /locus_tag="AC1ZCC_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIQ1630573.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYLQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
     gene            complement(1263..2060)
                     /locus_tag="AC1ZCC_002349"
     CDS             complement(1263..2060)
                     /locus_tag="AC1ZCC_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1630574.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYKDISKYSSVED
                     FFYKITKNQFETEKFISDLSKKYNFPYEKNSIIAKFLTDNHDKFKNN"
     gene            2262..2561
                     /locus_tag="AC1ZCC_002350"
     CDS             2262..2561
                     /locus_tag="AC1ZCC_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1630575.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            2826..3701
                     /locus_tag="AC1ZCC_002351"
     CDS             2826..3701
                     /locus_tag="AC1ZCC_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630576.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            3698..3916
                     /locus_tag="AC1ZCC_002352"
     CDS             3698..3916
                     /locus_tag="AC1ZCC_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630577.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            3903..>6127
                     /locus_tag="AC1ZCC_002353"
     CDS             3903..>6127
                     /locus_tag="AC1ZCC_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HIQ1630578.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     R"
BASE COUNT         2113 a          828 c         1145 g         2041 t
ORIGIN      
        1 attaaagcgt ttcgagcgtt tttttatttg tattttaatg gttggctacg tttttggcta
       61 cgtttggaat tattcgccca taaattttgc aaatcggtca ccagttttct taacagattg
      121 tggtgtgaca tgagcataga tattcatggt agttttaata tctttatgac ctaatcgttc
      181 ctgtacttct tttatcgacg caccactttc aaacaataag ctacagtggg tatgcctaaa
      241 tccatgtgga gtaatttgag gctccatcgg tgtctttttg taaatccagt ctaaccaatc
      301 gtttactact tggaggtagt acaacttgtt tgtgtttgta gtgaaaagaa actgttttgg
      361 tgaattcgtg ttatagccat ataagatcat tattttcatt tgttcttgtt gccaaatacg
      421 cagttgttta agtgtaaaat catcaagctg aatcgttctt gatgaatttt tagttttagg
      481 tacttgtaga actacttgat taaactcgtc ttgagcgata gttttaccaa ttgttattgt
      541 tttattgaaa tagtctatat cttcccattg aagcgccaat gcttcgcttt tacgcattcc
      601 tgtataagct aataaacgga aaaaagtgta cagtttaact gaattatttt ctttagtatt
      661 ctgtaaaaac agttgtaatt gatctttcgt gtaaaacttc aagggttgtt cttcttcaat
      721 ttttcttgga agcaatgttt tagacatagg attatcctta cagacttcca ttgctactcc
      781 aaatttaaat atttgagcag ttgtttttct caagaaacca tattgtttta atgttttgtg
      841 ccattcattc acctgttttt ggcaatagct aacacttatt ttatccaatc gtaattcccc
      901 aaaagcaggt aatatatggt ttttgcaaaa acgttgagca acggcaacag aagacggttt
      961 tactcttgtt ctgtagttat caatccataa ttcatacaac tcttgaaaag ttgtaacatt
     1021 gattttaaac tcttgttttt ctttaaagtc tacaagtagt ctattaagtg ctgtttgcgc
     1081 ctcagctttt gatttgaatc ttctacgctt tgtacgtata tcttttccag tcttgggatc
     1141 tttccctaaa taagtttcaa atccccaata tttttcgcca ttttgtaatt tataactaaa
     1201 aaatgttgcc atttgattct ctccttactt cattattgca ggtagagaag ttatttgaag
     1261 aatcaattat ttttaaattt atcgtgatta tcagttaaaa attttgcgat tatactattt
     1321 ttctcatatg ggaaattgta ttttttacta agatctgaaa taaatttttc tgtttcaaat
     1381 tggtttttag taattttata aaagaaatcc tcgacagatg aatactttga aatatcttta
     1441 taggctaagt ccatttcgtc taatagctgt tttaaagaat caatcattct ttctttttca
     1501 ccgtatttga aatcaccatt tcttatcttg tcttgaagtt tactgttggc tcttttgaaa
     1561 aatagctctt tagagataga gtgtctttgg ttagcgtact cttcaatcag aggattgata
     1621 ttattagcaa gtatctcatt tagataaatt tcaaaatctt ttgagtaata ttttgtaaat
     1681 atatttttta ttataggatt caataactca tagtctttat ctagcgaaaa cgtatcacta
     1741 tatctttctt gaatgtcttt aaagactttg tgaggtattt ctgggaagtc cttaatatat
     1801 agctcatacc ctatatcgat aaggtatgaa gttatatatt cttctaaagt tccccattta
     1861 atccagtcaa ttgttgaatt agtgatagtt gataataatt tcagcttttg tggatttggt
     1921 aaattggtcc cacgttccca gttggatatg gtcgcacgtg gtgaattatc tattttcctt
     1981 ccaagttctt ccatagtaaa accacatgaa aggcgtaatt gtttgattcg ttttcctgct
     2041 tctgaaggat ttggcttcat cttttttctc ctttttattt ttaaagatag tatatcatat
     2101 ttataaaata aaagtattta aaagtattta ctttttatct ggagtatgat actttattct
     2161 tataaataca aataaataca tttttttacg caatttgtat ttatttgtac ttataaaggt
     2221 agaggagtta tagaataatt aattaaaaag gagaggtttt tgtggaagca ataaaattaa
     2281 tgatttcaga agaacaggag gaaatgtttc taaagcagtt ttatcaaatt gctacaaaag
     2341 ctattgaaaa ggctcaagaa aatgcgggga tgacaaggga atatctcaat caaaaagaaa
     2401 tggcggaata cttaaatgtg agtgaaaact ttttaaaaaa tgctgtgaga aatgagggat
     2461 tgcccgtagt tgaattaggg gcaagaaaat tttattcgaa aaaggcagtt aatgagttta
     2521 tgtttgctcg aatgaaaaga ggggaaaata ttggaagatg atgaagtaga aattataaaa
     2581 aaatgcttta aaaaaagaaa aaaagtgttc ccaaaagttt gcagactggg ggaacactca
     2641 ataataaagt gctgagaaaa ctttatttat agctattata tcaaaaaaga aggtgaaaag
     2701 gtgttttctt ctggaaatgt tgtagttgac aaacttagag aaataaattt tgacggaaat
     2761 gtaatccaca cacatggtac gatcaattaa gaaaaaaaac gaaaaaaggt gttgagaagc
     2821 cctatttgaa tgcaatagtt atcttagcag aaattaccta ttggtataga cctaaaaaaa
     2881 tttttaacga tgatggtcag ctacttggtt atcaaaaaaa gtttcttgaa gatattttgc
     2941 aaaaatcata taagcaatta agcaaaaaaa caggattatc agaacgagct ataaaagatg
     3001 caattgtgta cttagagaaa aaggggatta tcaaaagggt ttttagaaat atttattatg
     3061 agaaaagagc agttacatta tcaaatgtta tgtacattgt tttaaatgta cataaattga
     3121 tagaaattac ttttcctact ttggattgcg aaaaaaagga gtatatgccg aaaattcgtc
     3181 agaggggtat cgaaaaaaag agatatgtct ctttaaaaaa aggtagaggt atgtacaaat
     3241 ttgtatatac gtatacaaag actacttcac agaacatatc aaagactact ctcgttatgg
     3301 aagaagagga tattaatata aagaggattt acagtaagat tagtgttctt tatgacgcaa
     3361 ataacgaaga tggtcgaaaa ttatttcaag tgtatcacta tttgagaaac gctaatgtag
     3421 atacagaaga tatcagaaaa atcattgaat atttatctga aaatactcag tatcttattg
     3481 ctgagtgtat tataaaacaa catgaagcat gcttttttca aagaaaaact gaagaagggt
     3541 tatacgatta tgtaacatat tttttgaatg gacttcagaa aagatataaa agtcaatttt
     3601 tagaaaatgt agagcctata gatcagttca aaaaagcttt cgaaatcaaa gagttacctg
     3661 aaattactat gcataattgg ttgaaaggag aagaatcatg attaaaaatc gcttaataga
     3721 tcaaatgtgg tgtcttgatc aaaatacaga tactctcgaa ttaatcgaga gtatctgctt
     3781 caacacagta gttctcgatt taagagctga aaatgacaat tgtgtaactc atatcataat
     3841 taatcaaaaa atggctcagc agctctctga gagcctaaga aaatgggctg aaggagaaaa
     3901 ctatgaaagc aattaaagaa aaaatcgtta gtagaaaaat aaactggtta gcactcagtg
     3961 tacttgtttt aatgattagc gttgttttta caagtaatat tgttgaagct agcattttca
     4021 attggtttga tgacacaaaa agtcaaacag agtttttgaa taattctctt tatagacctt
     4081 acctaaaaaa atacaatgga gatttcctta gtcagttcgg gatttggctt ggatgggctg
     4141 tggtaaaagg catgtttact gtgactgatt cgattcaaaa tatgattcca gatgtgttgg
     4201 atttgttcaa tttcattgaa agtacagggc taaataatgt ttatcaatca gtcataaata
     4261 caattgtcgt tggattaatg atcctttcat tgatgtttgt tggctacaag atgattactg
     4321 gaaaaggaac aatagattta agatctgttg gaatgaatat agtgatgtct gtagcgttaa
     4381 ttcttttaat gccaacaatg atttctagcg gtattcaatt tgccaaaata ttttacaatg
     4441 atgctactac gattacaaat tcagatgacg gagtcgcttg gtcattgata aaacaaggag
     4501 taacagatct ggcatatatt aataaaacag atcaatactc aagcattgat aaaacggagg
     4561 atcgaaataa gttaacacga aaaaattttc aacagacaga tttaacacaa gtgttgacgg
     4621 ataaagtgat tgataaattg gaaaaagaaa atccagctgc ggataatctt cgctatgaat
     4681 tagttgaaaa ttctaataat gaatttgttg ccacaaagtt ttctgataac ttcttatcaa
     4741 cattttctga tagcttgaaa tcaggttatt atcgatatca agcaaatctt tgggggattt
     4801 cgattgggtt gacagcgttg gcaatcgcct atgtgttcag cgcgtttgtg atcattacag
     4861 caatattaga acttgcattt aaacgtgtgt taggtgttct tgtttttgcg acagatattg
     4921 aaacaggaca acgttcaaaa gttgttttat ccgatatttt acaatgttac ttaacagttg
     4981 gatttcaagg gtttggacta tctatgtttg ctatgtttat caatttttta aatgctggac
     5041 aaggtatctc cacaaatatt tttataaaaa caattgctta tatctgtgct gtgtttgtgc
     5101 tgataaaagg cagtggaaca gtcatgagat actttggtgt tgatattgga ctcaaagaag
     5161 gctatgggca acttgcttct gcttttggta tgggcgcaat gctgtttaga aaaggatcaa
     5221 ctggatttaa tcgtgctaaa ggaagcggaa atggaaatgg atcagacagt ggagaaggag
     5281 aagatcgaaa accagaaaag aatttcggcg aaactctatc aaaaaaagct ggcaaaactg
     5341 gaagagcttt gggatatgcc catgaacgtg gattatcagg tttagcttca gatggagcta
     5401 caatggctag tgaaagagca ataaaaccat ttaagagtat gcgtgatatg gctaatgata
     5461 ccaagaataa gttcaaagaa ggtttagatg atggtacagt gtccgctatt aataaaaata
     5521 gcaaacctat gttagctaaa aataaagaag atgaaactgg aaaatatgct gattccatgc
     5581 caacacgtct ttcggataga aaagatggtg ctaaggttga aaatgccgat agaattatgt
     5641 ctagttctga acgtatgcgt gaagcaatga aaaataatgc tgaatcgaat aataatcctg
     5701 taagtgcaat tcagcaaaaa gttcaacaag atattgaaga acgtaaaaat gctatgcacg
     5761 gtcaagctaa gtctgctgaa gaattaatta atcagaagcg acaagaagct aaatacacgc
     5821 ctgaagcaat gaatcgtgag gaaatgttaa ggaaacgtgt agaagggcgt acaggagcta
     5881 atacggacga aaaagaagcg ttgaccaaag aaagaatcca agaagctaaa aactcaaata
     5941 ccggattaga aaaattggtc aaagaaaatc tacaaaattc tacaagtagt caaggtggcc
     6001 gatcagtaga tgtccgagaa aatattcaag gttcattgaa tggtcatgtc ggacggaatg
     6061 ttgatgtaag agagaattta caaagctctt caaatggtca aggtactaag acaattgatg
     6121 ttcgaga
//
LOCUS       DBNICI010000148         6123 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.150, whole genome shotgun sequence.
ACCESSION   DBNICI010000148 DBNICI010000000
VERSION     DBNICI010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.150"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(177..1139)
                     /locus_tag="AC1ZCC_002354"
     CDS             complement(177..1139)
                     /locus_tag="AC1ZCC_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIQ1630579.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1141..2580)
                     /locus_tag="AC1ZCC_002355"
     CDS             complement(1141..2580)
                     /locus_tag="AC1ZCC_002355"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HIQ1630580.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2765..4711
                     /locus_tag="AC1ZCC_002356"
     CDS             2765..4711
                     /locus_tag="AC1ZCC_002356"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HIQ1630581.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4975..5847
                     /gene="scrK"
                     /locus_tag="AC1ZCC_002357"
     CDS             4975..5847
                     /gene="scrK"
                     /locus_tag="AC1ZCC_002357"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HIQ1630582.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1944 a         1047 c         1084 g         2048 t
ORIGIN      
        1 tgtgtaaaaa tatcatttta gagcatgaaa aaaggagtgg atttctccac tccaaaaaag
       61 tctaactttt gggggtcact acaagttgac aactcctttt tgtttacaaa tcaattggat
      121 atctacaaat tgtattttct aacgaatttt atagacatta aaaaattaat ttaacgttaa
      181 atacttcctc cagaaagtat tgagattggt aaaatataat ccttatttgt cttttcgcct
      241 ttaatttttt tcagaagaat atcaacaata agacttgcaa tttcatcaat tggttgtctt
      301 attgttgtca attgtgggaa aaatttctca ataaaagagg taccatcgta tccaataatt
      361 tttaaatctt ctgggatatt aagtttaagt tgatgagcaa ttttcatagt gagaatggct
      421 gttaaatcat cagaaacaaa aataccatct ggtttgtgat ttataatagt tgactttatt
      481 tccatttcac gacggatagt agacagatta ttaggaattt tgaaaatttt tccatttgga
      541 ttttgaaaag aaaaacctag tgcacgtaat tctgtgggag aatccgtgtt atcatttcct
      601 gtaatcataa tggtattttg acaaccattt ttttgtaaag tcttagctgc aagttttccc
      661 ccttcaaagt tatctgaaga aacaatggaa acattaggag ctaaatttct atcaaaagca
      721 acaataggtg ctctcacacg ctcataatca gcaattccaa gattatggct tgatgagatt
      781 atgccatcaa cttggttggc ttcaagcatt tcaatgtatt ctctctcttt agcaggatca
      841 ttttgactat tgcaaataat cgttttatat ccatgtttaa ataactctat ttcgagatac
      901 tcaatgagtt cagagtaaaa gatattacta atattaggaa aaataagacc aataagctga
      961 ggtgactttc cttgaagtcc acgtgctaaa ttatttggtt tataaccaag agttttcata
     1021 gcttcatgaa ctttttgttt tgttttttct gataaatatc ccttattatt aatgacgcgt
     1081 gaaacggttg ttgggctaac tccagctaat tcagcgacat cagttaattt tgctaccatt
     1141 ttaatatttt aattcaaagt attttccagt aggttcgcct gatttgattt gaataccatt
     1201 ttggtgattt tcagggaata cacgactagt aaacactttt tctcctttat taataaaaat
     1261 ctcaatgatt gagttatcta caaaaatgtt agcagttgtt tctttagtat caattgagca
     1321 ttcacgaatc gtaccaaagt cagtggcata ttgaatgcct gctttgctgc gatctaaaat
     1381 aatttttcct tctttggtat ctacaatcaa gctaagtcca tttcctttac tatcagagaa
     1441 aagaagtaac tccgatttct gatttggagg aaaagttagt tcaagctcat aagtattatt
     1501 tgtttctgct tttgctgtaa attgttcaga actagcacgt aatgatgtaa tagcttctac
     1561 tggatattgg taaagtttac catcttttat tgaaagttct tttacaagac tcattgctcc
     1621 ctgataatca tacttatcag ttggataatc gatatcaggt agtccaatcc aactgacaat
     1681 cagagcacga ccatcaggag tattgaatcc ttgagtcgca tacgcttcaa atccataatc
     1741 aagattttga atttctgaag cgcccacaag ttttactttt tctatgtcaa gcgattgaca
     1801 gatcttaaaa gtattcggat agatattccc ataatttagc tcagatttat ccaacccttg
     1861 aggacaataa atgagaacag gttttttgtc tacaaatact agatttggac actcaatcat
     1921 gtattctgaa ccagtgccgc caaaatcaag gttgccaact tcctcccatg tttccacgtt
     1981 attgtcatcc gctttgtaaa gtttgataaa gcctgatttt tctaaatttt gtgctccaac
     2041 aatcgcatac aattgtcctt cataattaaa catttgtgga tcacgaaaat gatcagttac
     2101 atcagcaggt ttatgaataa gaacattctc acctttcgta atgttgtagt ctttatccat
     2161 ccatgctccg atttgaagtg tatcacgttc ccaattttca tttcgaacgt ttcctgtata
     2221 gagtaagaat agattcccct ctatttcata tgcagaaccg gaataagcgc cgtggctatc
     2281 tcgttttgtg tctggaagaa gttctgctcc tgttacatga aaatgaacta aatcatctga
     2341 ttcattgtgt acccattctt tcaacccatg agcagctcca aatggccaat tttgataaaa
     2401 aagcgtatat tttccgttaa aaaaagaaaa accattagga tcgtttaaca atcctgtttt
     2461 aggttcgata tgatagctag cacgccaagg tgatcgtttt acattatcct taattttatc
     2521 catctcatct ttagtccaat cagaataagc acgatatcgt atttctcgag gtaagttcat
     2581 attttactcc ttcaatttat ctttttacaa ctagtatggt aaacgttttc cgttgaaaaa
     2641 tcaatcattt catttaaaaa aataaactat atgacaaacg tttgacatat ttttaagata
     2701 tgctaaaata aatttgcatt tcagtgaacc gttttcacgt aaaaataaaa aaggagtgat
     2761 tcgaatgaat tacagtaaag tagcttccga cgtaatacaa gcggtcggaa aagataacct
     2821 tattgcagct gcacactgcg ctacacgtct tcgtctcgta ctaaaagatg acacgaaagt
     2881 gaatcaaaaa gctttagacg aaaatccaga tgtgaaaagc acatttaaaa ttgatggaca
     2941 atatcaagtg attatcggtg caggtgatgt taatttcgtt tatgacgaac ttatcaaaaa
     3001 aactggactt tcggaaattt caacagatga tttaaaacaa attgctagta aagataaaaa
     3061 attcaacccc attatggcat taattaaatt attatcagat atttttgttc caattatacc
     3121 tgcccttgtt gcaggtggtt tattaatggc attacaaaac tttttaacag ctgctggact
     3181 ttttggacca aaatcaattg aagaaatgta tcctgcgatt gaaggtattt cctcaatgat
     3241 ccaactgatg tccgctgcac cattcatgtt cttaccaatt ttagtaggta tttcagctgc
     3301 taaacgtttt ggagcaaacc aatttcttgg tgctgcaatt ggtatgatca tgacaacacc
     3361 agatcttggt ggctcaactg aatattggaa tatttttggt tatcatgttt cacaaacaaa
     3421 ctatgcttat caagttattc cagttttagc agctgtttgg atattatcta tattagaaaa
     3481 attctttcat aaaaaattgc catcatctat tgactttaca ttcacgccat tattatcagt
     3541 aatgattact ggatttttga ctttcacagt tattggccct gtaatgttat tgttgtctaa
     3601 tggaattaca gacattattg tttggctata taacactact ggatttattg gaatgggtat
     3661 ttttggagga acatattcac ttatagtcat gactggttta catcaatcat ttccagctat
     3721 tgaaactcaa ttactttcag cgtggacaaa tggtatcgga catggagact ttatctttgt
     3781 agttgcatct atggcaaacg ttgctcaagg tgctgcaaca tttgccattt ggttcttaac
     3841 taaaaattct aagacaaaaa gtcttgcttc ctctgcgggt gtctcagcac ttcttggaat
     3901 tacagagcct gccttgttcg gagttaactt aaaataccgc tttccattct tttgtgcttt
     3961 aattggttca ggaattgcta cagcagtagc aggattactt aaagtagttg ctgtttcact
     4021 tggttcagca ggtttcttgg gatttctttc aattaatgca acatctattc cattttacgt
     4081 attgtgtgaa ttgattagct ttgtagttgc ttttgcaatt acttacttct acggtaaaac
     4141 aaaagctgct ggtatcttta aagcagaaga aattgctgaa caaacaccag ttaattcttc
     4201 aaaagtagca acaacagatc caatatctac tgatgttgaa tcaacacaaa caaaagtttc
     4261 atcagaaaca attgttagtc ccatttctgg taaagtgatt agtctttctt cagtaaatga
     4321 tccagtcttt tcatcagaag ctatgggaaa agggattgcc attaaaccaa atggaaacac
     4381 ggtccattct ccagtagatg gtactattca actcacgttt gaaactggac atgcttatgg
     4441 tattaaatca aataatggtg ctgaagttct aattcatatt ggtattgata ctgtatcaat
     4501 gggaggaaaa ggatttacac aaaaagttac tactaatcaa aaagttagaa aaggcgatat
     4561 tcttggagca tttgaccgtt caatgattga gaaagaaggt cttgatgata caacaatgat
     4621 tattgtgacc aatacttcgg attattcaga agtaaatata gtagctgaaa atacagtcac
     4681 tgagggtact gatttacttg aaatcaaata aatgtatttc cctgagtaaa gatggaattt
     4741 tctttccaaa atcaaataat atagattaat caaatatttg agaataacat tttatttaat
     4801 cttttcatat atcaattgta tgaaaaatta ttagaatttt aaaaaatgat aaaaattcaa
     4861 aaagaaaaaa tagtagttat ttacttgtca tgttactaaa taaaaaaata atttaaatat
     4921 gatatatagt catttattta gtgatttcat tctaaaataa agggagaaat gtctatgcta
     4981 tacggtgcaa ttgaagcagg cggcacaaaa tttgtgtgtg ccattggcga tgaagaaatg
     5041 acgatcaagg aaagagtgag ctttccgaca acgacacctg aggaaacaat gcctttagtc
     5101 attgacttct tcaaacaata ccaagcagat ttagcaggaa ttgggattgg ctcatttggg
     5161 cctatcgata tccatcgcga ttccgccact tatgggtaca ttacctcgac cccgaaattg
     5221 gcatggcaaa attttgattt catcggtacc atgaaaaaag aatttccgat cccgatttct
     5281 tggacaacag atgtcaatgc ggctgcctat ggtgagtatg tcttcggcag tggcaaagga
     5341 ttgagcagtg tcgtttacta tacgatcggt acgggtgttg gcggcggtgc cttacaagaa
     5401 gggcgcttta tcgaagggtt tagtcatcca gaaatggggc atatgctcgt cgtccctcat
     5461 ccaaaagatg actttgctgg cagctgtcca tttcatggca actgtttaga agggatggcc
     5521 gctggacccg ccatcgaaaa acgcttaggg aaaaaaggac aagaggttgc agaagatgat
     5581 ccttattggg aaatcgaagc ctcctatatt gctcaatgtg cctacaatac aaccttgatg
     5641 ttttctccag atgtgatcat ttttggcggt ggtgtgatga aacaacgcca tatgctgcaa
     5701 aacgtccacg acgcatttgc aaaattggtc aatgggtatg tcgaaacacc tgagttaagc
     5761 aaatatatcg tgaccccagc attagaagac aacgctggaa cgctcggttg tttggcattg
     5821 gctcgagaag cggtcttaca ttcataaacg aaaaaaggct cttcgacatt tagtgttggt
     5881 agagaaagat acaactgaaa atttagtcat aaccagttag gcagagtgct taactggttt
     5941 ttgttttact tggaagatca agccttgaat gagataaatt cttgctcgaa agttatggaa
     6001 atttcgatag ccgtaggcca cttgtattgt gcaatagttt tttacaaaaa aaagggggtg
     6061 cggatatttt tttggacctt tttaacaaat aggttctgtt gcaaagtttt aaatctacta
     6121 tca
//
LOCUS       DBNICI010000149         6081 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.151, whole genome shotgun sequence.
ACCESSION   DBNICI010000149 DBNICI010000000
VERSION     DBNICI010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.151"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            481..567
                     /locus_tag="AC1ZCC_002358"
     CDS             481..567
                     /locus_tag="AC1ZCC_002358"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HIQ1630583.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(688..1377)
                     /locus_tag="AC1ZCC_002359"
     CDS             complement(688..1377)
                     /locus_tag="AC1ZCC_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630584.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1384..1662)
                     /locus_tag="AC1ZCC_002360"
     CDS             complement(1384..1662)
                     /locus_tag="AC1ZCC_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630585.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1622..2536)
                     /locus_tag="AC1ZCC_002361"
     CDS             complement(1622..2536)
                     /locus_tag="AC1ZCC_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HIQ1630586.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2518..2898)
                     /locus_tag="AC1ZCC_002362"
     CDS             complement(2518..2898)
                     /locus_tag="AC1ZCC_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HIQ1630587.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3348..3635)
                     /locus_tag="AC1ZCC_002363"
     CDS             complement(3348..3635)
                     /locus_tag="AC1ZCC_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HIQ1630588.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3655..3879)
                     /locus_tag="AC1ZCC_002364"
     CDS             complement(3655..3879)
                     /locus_tag="AC1ZCC_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HIQ1630589.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4334..4504)
                     /locus_tag="AC1ZCC_002365"
     CDS             complement(4334..4504)
                     /locus_tag="AC1ZCC_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630590.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4519..5052)
                     /locus_tag="AC1ZCC_002366"
     CDS             complement(4519..5052)
                     /locus_tag="AC1ZCC_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HIQ1630591.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5073..6017)
                     /locus_tag="AC1ZCC_002367"
     CDS             complement(5073..6017)
                     /locus_tag="AC1ZCC_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HIQ1630592.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
BASE COUNT         1591 a         1129 c         1034 g         2327 t
ORIGIN      
        1 attctgtatc cattggtaga gtaaggacac agcttacggt aaagaggttt aaaaagaggt
       61 ttaaaaagag atataaaaag aggggggcta attttcccgc ctaaaaacca cttctttttt
      121 ctttaaacag gttcgcgcat ttttcaaaac tgccaatcca aaaccttgct tcctacggtc
      181 gccaaaaagg tgtgatcgct acgctctcac attgtactgc gcagtcgcct aaaggctcct
      241 ttgctaaaaa catgttggta aagaggcctt tgagcggttc tgaatgctta tctaagccgt
      301 tttaagacag tcttggataa aaatccttat ctagctttaa agcgccttac aggcggtcag
      361 ggcaagccct aacaacccta aaataagcga tacttaaaaa caccacacaa gtttaatttt
      421 ttaagatata ataacttgtc gcctatccga cagtcttgcg actggaagga ggtgtatcta
      481 atgtttgaat tgttcatttg cccccttctt atagaagttt gtgtctcttt attcagttat
      541 tggttgaata accgaaacaa gaagtaaaat caataaaggc gacatcaacc tgaaaaaaag
      601 ccccagagaa tccgacctct ctggggcttt cgtgtatcta atgtttgatc atttgcatgt
      661 ttagtttaac atgattgccg atcaaattca attttgctgt tgctgatcta accattcctt
      721 gacacttttt agataattta atgccgtttc ttttttcgtt ggattttgta tgttcgttgc
      781 ttgctgaagc agtgctttat aacgttcgta ggtaactgat tgttgccgtt cttttcgttc
      841 ctgttcacta gttgaaaaag ggattgtatc actgaataaa ttataacaag cttcttctgt
      901 ctgcgtatct ttaaaatatt gcacgtcttt tttggttcgc tcatagttgt cttgaacctc
      961 tttttgctgt ttttctggca agttttgata aagacgagtg agtttttcca tgtttttatc
     1021 cgtcaaacgt ttttcttgat tcacaaaaat atcgttcatt tcctcataac aagtggtaaa
     1081 ctcttccatt ttttgtgtta acacttgctc ctttttcttt ttatcacatg ctgcttcata
     1141 cttttgagtg atatccccta ataaaatact tcttgaaata cgtttatttc cctctaaaac
     1201 gccgaataaa ccacttaaaa cgattgataa tgtaaatata acccctggca ataaaaacct
     1261 cttaaatcga attttagagc cttttaatga atatcttgtt tgataagcct tatagctaag
     1321 ccaaataatt cctaaaatac tggcaaaaat gagtagatag ggtaaataga atgccatttc
     1381 tatttattct ccccatacca gccaaaaggc taacagtgtc atgattaagc aacataaggg
     1441 ggcaagaata tccattgatc gctgcataat tacttttttc tgttctttat ttcgctggtt
     1501 taatagcgca tggtggaatt ttttgacttg tgaatcaatt gtagtttgta ttctttcttt
     1561 ggtataaggt gcaagaaaag cacgttgttt ctttatttca ttttctatgt cttgtaacgt
     1621 tttagaacga taattcttgt tggtagtctg ctcctctttc aacgtgttgg tcatggtttc
     1681 ttcgagtcgg tctaatttcc ggttgttcag tcgtaagagt tcgttgttct cttcgtactt
     1741 cttttgcagt ttcgcgaatg ctagctcgag ctcttcgtag ctcgttggtt cgttgttgat
     1801 tgtaggctcg ttctcgtctg tccagctcat gtaaaatcgt ctccttttcg taatcctttc
     1861 ctagtttgtt ggatcgtact tttttctttt tgtggatcaa ttcgtaagta aaatttttcc
     1921 cacgattcaa gcaatgaatg ccgaatgttt taagagaatc acaaaattgg ttatacgttc
     1981 gagttttttt gtttttcatt gtttgatcaa tcgcaaaccg aatttcgtct ttccaaacag
     2041 gttcattttt ttcttttaat ttgatttcag acatggttcg tttttcgggt ttcttccctc
     2101 gttcagtgat tgttaagtca tgttcaagac aaatctcgtc atttaggtca taacatttgt
     2161 ttaaaaatgc ttgaccatgg gcttgaaatt tgttcccagt ttccagattg acggagttaa
     2221 tgacaatgtg gttatgaata tggtctttgt ctgtatgtgt gtaaatcgcg atttggtggt
     2281 caggggctat ttttttcgca tactctgccc ctagttgatt ggctttttca gcggtaattt
     2341 ctcctggtcg aaaagactgg atcaataagt gtgcttgtac acgatcgtct tttccataca
     2401 gcatgcgaat ttgtttcatt tcgcttttgg cataatcaat gtcacagttt atccctgttt
     2461 tgacggttgc tcgtggttcg gcgtaattga tcgctcggct acaagaagtg gttcggctta
     2521 ctttaactgt tgccatatct tttgtagctc cttttgcatg atttttaggt tttcggataa
     2581 gcgttgggcg tcgccaggat caacgtcgtg ttggtgtgtg ttgcaccact tggcaatttg
     2641 attgatattc ccgccagctt tggctaaatc gcgcgcgatt gcctgagcaa tgtctggcgc
     2701 cactttcggt gtcaccagac gcgcgttttg tgccttggct ttcacgaaag ccggcacact
     2761 catttgcaaa gcttcagctg actgcttcag ctttgcaaat tcggattcgc tcactcgaaa
     2821 actgatttgt tttggctcgg atcgataggt atatttttta gaatgattgc tagccaaata
     2881 ttgattttgt tcgctcataa gtcccaccct ttcagtacgt cctacggacg tatcgttcgc
     2941 aataccattg tacctaaaaa ggtacaataa gtaaagctca ctaaagctca ctaaagggct
     3001 tggcaagctc tatatttgct agccaaatac ctacgtattt ggtgcaaata accgcttgtc
     3061 aaaggggcgc aagctccccc ttgaatcccc ctccccccca ctctccacaa tacaaaaaag
     3121 actagcaaat gctagccact ttttgtaaag tttcgcttgt ttcaccttcg gtgaaattgt
     3181 tgcaaatcaa taattaggta gagctacatt tttttcgttg ttcaaagatt cttatgccat
     3241 ttactattaa taaatctata aatatgataa tttttcggct ctataattta tgggactgat
     3301 gaatcagaat tgattcttca gtcccatttt cattttattc caaagaatta actccaaata
     3361 ccaatagaag cccatcctct atatacttga gattccttac taacaatttc ttgtaattct
     3421 attaaagatt taggtaatcc aaatttatgt tttaaaatat atcttccaag aaaactactt
     3481 aatttcatac taacttcacc aacacttttt ccctcactga tttctttaga ataaagaaag
     3541 agcttttctt ttaagtcagg atagtcatct atagttgctt cattgtatac tttactaatt
     3601 gcatttaaaa gtttttcttc tttagtaaaa tccattataa taacctccct accactacct
     3661 tctaggtgcc catggaccat gattaaccca accattaaca ataatttttc caatttctcc
     3721 tttagcttga ttccaatcta cccaacattt ttctttgtta caataaagac catttccata
     3781 gtaagtagct gcatcaactt ttattcctgt accgatgcca gctagacaag ttcctaatat
     3841 tcctagaata acacaatgtt ttaatacttt ctttttcatg attgtttttc cttttcgttt
     3901 ttttaaaatt gcttgttata ttaaataatc ttagtaattt tattatataa cattttatta
     3961 tataacaatg attaacgtca taaaaagtag ttttgaattc ataatcgttt aaataaaatt
     4021 ataagatcag ttatttaaac agctagtaga cataatgtgt atactaccaa gtaacgcctg
     4081 taccaaggga tccaggcgct gattttgtga atttttattg gtctaggtta aaagctcttt
     4141 ttaaggcttg gttttctgcg gttgtttctt tctaattctt tgtgaatatt gatcggtcat
     4201 tttttaaata cttgttattt ttgtttgtgt ttgttatgct ttttacatat ctcatattca
     4261 tttattattc ttttctaagg ttaataatag gataatctct ttcagaaaga ctgctcgtac
     4321 gagcagtctt ttcttattta tcaagttttt ctacaaatac ttcctcattt tgactgttgt
     4381 agtaattagg attgagtttt agttcccctt ttttatttcc tttgttggcc actgataata
     4441 acattgggct aaagcttaat attcctagta ggatagaacc gaatagcaag tgtttttttg
     4501 tcataagtat ttccttcttc atttccatag ctgccaccat tttttccaat tctttgtttg
     4561 ttcgtccatt gtttctgttg cttcagaaag agtcgttgaa ctattctctg aagactcttc
     4621 cgtataagtc aactgcagtt gctcctgaag tttttcgatt tgttggttgg cttgcagggt
     4681 gagttgttgt tgctggtcga gtagtttttg tatatgagaa ttattttgct ctttgagggc
     4741 aagttgttct cgtaaaaatt cattttcttc acgcaactga tctagcaact tgtttgcaac
     4801 cacgtttttg tttggtatat aagcattttt atcgttgttt tgttcatgtt tatgcaactt
     4861 atctagcaac ctttgattgt ctaagttgcg agcaagttgc aatactccag aacgcaatac
     4921 aaccttataa ccacgctcat ttttagctat catggaagaa tctaattttt ggatatgttt
     4981 cgatattgct tgcctactca tcttaaaaag atctgacaat tcctttattg taaaggtttc
     5041 ttgtggttgc atactggttg cctcctaggt tgctactgga ataaattatc taaatcagat
     5101 aataaacttt ttttctcttt ttccgaaagt gcggactctg tttgtttttc aagagctttt
     5161 ttctcttttt ctagttgttt gatttgttgg aggagttcaa gttctcgttt cttctgtttg
     5221 attttttcgt taattgttgt agcgggttct tcgtattctt tttgtttatc agctaaagcc
     5281 aatgctttat tttggtcttc tttggttggt gcatgtttgt taaagtcgtc tgcttttttg
     5341 atctcaggtt tgaacgtaaa ttgatagcca ataacaggtt ttccccgtcc ttttccgtat
     5401 ttctttttga ttgttagtcc tttgaagatt gcagttagtt cttgacgtat aggttttata
     5461 actcgtttat ctatatcagg ttgcttataa cttttaggaa tatctaataa ttcaagaaag
     5521 tcttctttgg agaaatatgc gaaacctttg gttctgtatt gctttaacag acgaaacata
     5581 gtctttgcat agccactttg tagctcgttg aattgttgta aagaatagcg tacccactcg
     5641 tctaaattat ttaacaaagg cagtgctttt tcgtaaattc gtatatctac gtaaggattt
     5701 tctacgtccc cgttgatttt aaattctgta aacattacaa acatatcgta agaaagccca
     5761 gactcacttc tacgaccaaa gcgtaaagat aagatttttt cataagttgc ttttaaatct
     5821 tctacaaatc gtacatttgc agttgctttg tagttactta aatcttttag ttggtcaaac
     5881 gtaaaacgaa ctgtcttatc ccctttttca cgcattctag acacgatgga aaaaaacaaa
     5941 ttcatttcaa ccggtgtaaa ttttcttaaa ggaattgtat ttaattcatg gtggtatttt
     6001 acaatctcat tagccatata atcacctcaa tccaattatg aatgacattt tttaaatgtc
     6061 aagacattca tatttgacgt t
//
LOCUS       DBNICI010000150         6042 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.152, whole genome shotgun sequence.
ACCESSION   DBNICI010000150 DBNICI010000000
VERSION     DBNICI010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.152"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(406..780)
                     /locus_tag="AC1ZCC_002368"
     CDS             complement(406..780)
                     /locus_tag="AC1ZCC_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIQ1630593.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(803..2203)
                     /locus_tag="AC1ZCC_002369"
     CDS             complement(803..2203)
                     /locus_tag="AC1ZCC_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HIQ1630594.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2207..2617)
                     /locus_tag="AC1ZCC_002370"
     CDS             complement(2207..2617)
                     /locus_tag="AC1ZCC_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIQ1630595.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2635..3126)
                     /locus_tag="AC1ZCC_002371"
     CDS             complement(2635..3126)
                     /locus_tag="AC1ZCC_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HIQ1630596.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3136..3927)
                     /locus_tag="AC1ZCC_002372"
     CDS             complement(3136..3927)
                     /locus_tag="AC1ZCC_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HIQ1630597.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3920..4693)
                     /locus_tag="AC1ZCC_002373"
     CDS             complement(3920..4693)
                     /locus_tag="AC1ZCC_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HIQ1630598.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4849..>6042)
                     /locus_tag="AC1ZCC_002374"
     CDS             complement(4849..>6042)
                     /locus_tag="AC1ZCC_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HIQ1630599.1"
                     /translation="KFRMKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVK
                     IPETHCLIVMHGKITASAIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSID
                     TTNGLILLVDMGSLEQMYEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQ
                     MDFDSFQVKVQYYKGLSQKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNAL
                     KKIAKEKDTMIFKNTICVLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEF
                     CLNELVKLFTIEGASTKLRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLS
                     GMIERIMIGDGITNEHLKEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIF
                     EQTIF"
BASE COUNT         1936 a         1206 c          780 g         2120 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tcttcatttg
      421 gcttatctct ataatctcat gatttggtcc gtagaaatta aagtaacgga ttccattttc
      481 ccagaacggt aattccataa tatttttatc taataattca tatcctaaat cttgaattga
      541 attgaatacc tcatctatgt catccgtatc taatgctaga tgatcaatgg ctcctctaca
      601 ctcagtagtt ttatcttccc atatttctat tatcgttcca ttcaactcga aaaagccgac
      661 tttacttccc acattcattt cttcattaat cagactaaac cctaaggatt cataaaagat
      721 ttttgaagct ccaaaatctt ttgttggaat tccaatatgt tgtaaaccat ttaatttcac
      781 ttacatcatc ccttcaattg ttttaaaatc cacattcata atttattgaa tagtctagtt
      841 tatctacctt gattgctccc tccgtaacag tcatacttaa tgctgttccg actttatcat
      901 acattctagt acttaaagca acattatcaa tatataaaac agctatcgta tcatcaacaa
      961 ttaactttag ttcatattct ttgcctattt ccaaaaatag tggtcgttgc agccccttat
     1021 caaacatttg aggccaagga tagttcggag tttttccgaa agtaagctga ttattaatca
     1081 atgaaatacg aaattcgtat ccttcttgag tatcactatc tacaaataat tttaaagcaa
     1141 actcctttgt taacgatgta atagtcaaag ttgtttcaaa tagaaaataa tcttcgattt
     1201 taggaacaat tactctttct ttttttccaa attctttctc taacttaaaa cttgaaaatg
     1261 gagtgatttc tttaaaattc tcaattattt tttcaggcag tttaacacca agagttttgt
     1321 ctgtcttttg aataatttga tgtggaacaa gtgcaccgcc ccaatcccag ttattttgat
     1381 cgttctcccc ttcttttgtt gccacccagc cagttagaaa acggccagtt tcattagttc
     1441 cgactgttct tgctgcataa taggcttttc cgtcgaaagc ttcgtctact ggtgatttcc
     1501 aatctgaaaa taatcctgct ccgactcgat atttagtctt tttatcttca caatactctg
     1561 aaaaaagtaa ataccacgtt ccatcaattt caaatatatc aggcatttca atcatgttaa
     1621 attcattatt agcccagtag tctcctttga aattccaatc ttttaaatct tcggattcaa
     1681 aatagaccaa tctccccgtc ggctttttct tacttgctgg ttttctagct cctagtatca
     1741 aaacgtattt tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa tcattcttat
     1801 catatccttc ttgaggaaca agttttgctg cttcgccatc ctttttccaa tgaattaaat
     1861 ctttagaact tgcaatcatt aacacttctg aagtttttcc atccaactta gctttgcgat
     1921 tatgtccagt atacaaagct aaatactcat tgattccttg gacaatactt ccagcgtaaa
     1981 tgtattgatc aatgtcatcg ttgccaccac gatgaattac ttccccgtat tcttcaaatt
     2041 tcacaaaatc agtggttgta actagtgacc acccaaaagg atcagttaaa ggtttaggat
     2101 ttctcgtatc tcgttgatga aatagataaa aagttccatc tttataaaaa ggcatgcaat
     2161 ccccaaacca aaaatcttta ggcttgtaaa aaattttttg catttatcac tcatcctctt
     2221 cgttagtatg tctttgcata tctgcaagac acgcaattgt attcatattt cttagatttt
     2281 cgatgatatc atcgcttttt tttccttgta atctcaaatt gacagcctca atcaaaattc
     2341 ctaaattcat gccaccaaga agttgaaacc cattttgaaa tacttcactt cttttagcaa
     2401 attcgttgaa aggtgttcca ccaatcaagt cagcaaatac cataacttct tgatttttta
     2461 cttgagaaac tattttttca atgttttcac ggaattcttc aatgcctgca tctccaagtg
     2521 ttaccacatg acaatttaaa ttatcgccaa aaaacatttt taatgtatct acaaatccaa
     2581 tggcaatatt tccatgagat gtaattataa actccatcca ctcatcctct gttcttaata
     2641 aataaaatcc tttaaaaatt cttttgaatc tctaggagtt tgtatcactg tcacttcaac
     2701 tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg
     2761 acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg
     2821 taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt
     2881 ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg taattattga
     2941 agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc
     3001 tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc
     3061 tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc
     3121 atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa
     3181 gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc
     3241 tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat
     3301 tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag
     3361 tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt
     3421 ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc
     3481 agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc
     3541 caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact
     3601 atctccaatt cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa
     3661 agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt
     3721 atacggcgta gtgttaaaaa attgtaagtg agctaaaacg gattgtttca ttccttcagg
     3781 atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc
     3841 cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt
     3901 gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta
     3961 cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg
     4021 aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg
     4081 caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata
     4141 gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc
     4201 ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg
     4261 tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga
     4321 atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta
     4381 caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg
     4441 agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac
     4501 caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt
     4561 gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga
     4621 ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt
     4681 gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg
     4741 ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct
     4801 aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt agaaaatagt
     4861 ctgctcaaaa attgtataga ttaactcata ctctcctttg ggtatttcaa tacagtaaat
     4921 atctttgatt tctgaaaaaa aatcttttga tacatttaaa aaagtatcaa atagttgcgc
     4981 tccctctttc aaatgttcat ttgttattcc atcaccaatc ataattcgtt caatcattcc
     5041 agaaagatgt agaaatagat taattctcag aaaacttggg attttgacat cgtaatattt
     5101 ttcatacaaa tatagtactt tttctatctc attgaatact tttttggaat ctaaaaagcg
     5161 aagcttagta ctagcgcctt caatagtaaa gagtttaact aattcattta agcagaactc
     5221 acagctatcc gagtccatat acttatttaa tttttctaag gtttgattcc cattaattag
     5281 attttctaaa tttaaacaat cgattccttg aatgtaaaat tcagtggttg aaagaacaca
     5341 aattgtattc ttaaaaatca tagtatcttt ttcctttgct attttcttta atgcgttaaa
     5401 atcaaaaatc aaaatctcca cttgattatc gagataccgt tttaaaatat ccttaacttt
     5461 ttcagaaatc ccctcaccag agagacaaga tactattaca tttttctttt gtgataatcc
     5521 tttataatac tgaactttaa cttgaaacga atcaaaatcc atctggtaaa aatgactaat
     5581 tggctttttt tggcaaactt gaatcccaca ttcaatcgct aaagctgtag aaacattgtt
     5641 taaaattacc aaatcaccta ttacattgga ttcaattttt tcatacattt gttccaatga
     5701 tcccatatca actagcagaa tgagtccatt cgtcgtatct atacttttac aaaaattgtt
     5761 gattaggtta attaagtcac tagtctcacc ttcaattggc atatcgaaag cctcataaac
     5821 atatgtattc agcaacttat ttgtttccct tgcgatcgca gaagctgtta ttttaccgtg
     5881 cataactatc agacaatggg tttctggtat tttcacatag aacttcttta caagaaaaag
     5941 tatccatttc aacaaaagtt cttgtttact ttcttcgact tctaaactac tccaaatctc
     6001 atttgccaat aatttttgtt tatcattttt catacgaaat tt
//
LOCUS       DBNICI010000151         6001 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.153, whole genome shotgun sequence.
ACCESSION   DBNICI010000151 DBNICI010000000
VERSION     DBNICI010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.153"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(997..1272)
                     /gene="prgO"
                     /locus_tag="AC1ZCC_002375"
     CDS             complement(997..1272)
                     /gene="prgO"
                     /locus_tag="AC1ZCC_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HIQ1630600.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1244..2197)
                     /locus_tag="AC1ZCC_002376"
     CDS             complement(1244..2197)
                     /locus_tag="AC1ZCC_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIQ1630601.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2809..4302
                     /locus_tag="AC1ZCC_002377"
     CDS             2809..4302
                     /locus_tag="AC1ZCC_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HIQ1630602.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4437..4733
                     /locus_tag="AC1ZCC_002378"
     CDS             4437..4733
                     /locus_tag="AC1ZCC_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HIQ1630603.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            4834..5685
                     /locus_tag="AC1ZCC_002379"
     CDS             4834..5685
                     /locus_tag="AC1ZCC_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630604.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            5706..5918
                     /locus_tag="AC1ZCC_002380"
     CDS             5706..5918
                     /locus_tag="AC1ZCC_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630605.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         2052 a          953 c         1074 g         1922 t
ORIGIN      
        1 cgtttgataa atgaggttgg tgttgaacgc ttctctttct atgtagaagc tgcagcagga
       61 taccataaat acgaatacta tattggctat taaagtgcaa taaaattata tttcttttat
      121 gttttacatt ttgtatcaat tctgataaaa ttacctatag gtaattaaaa aatataaaaa
      181 aactagttac aaccttagga cgttgtaact agcttgaagc atactagagc taactaaaat
      241 agcactagaa agcaatgtta aacccgtcca acaaacttga tttaacattg agaccagctt
      301 attcagctaa atatagttga ataagcttct tttgttttaa aaaaacaaaa ggtgtactac
      361 gaataacgca agtaaaagaa atactattac aaccagtagg ctacaagtaa gtattactaa
      421 acattgcgta gttagaacct ttgctctatt gtctaaagcc acaaaattag agatcagctt
      481 tatgatgcta agagcgagct gcatccatgc ggacataggt tcttttgaca ctattaattc
      541 tcctttttag gataagggaa attgctagta gttttttcat tagcaccatc ccccttcatt
      601 tgattgagtc tagtgctgct gtgatagttt gctgtttaat atgcttacat tcttattata
      661 acataaatga aacaaacaca tggttagagg tcgtatcaat caaaatagtg tctcacaaac
      721 gatttattac ttactcctgc tgactaacgg actgttcttc agtaactgta actatatgtt
      781 tgttaaaacc accattacct gtagataaaa accaatatat ttactatact cactatatta
      841 aatatattta gtatagtaaa aactagaaac aataaaattt gtgaatgttg tataccccca
      901 aacaatacac atattttgaa ggattaggat taataaagct aaaaatatag taagtatact
      961 aaatatattt actatatcta gtatacttac tatattttaa tcttttgatc gtctattata
     1021 tataatccgt tcctcgtcgt ttaaagtatc aacataggcg tccattgcaa gtttgactat
     1081 ttcatacata ggaatatctt ttgcataact aagatcccga atggttttta gtacgggagg
     1141 atctacttga actggttttc tttctttcgc agtgtacttt cgttccccta actcgacatt
     1201 aatatcatct ttaattgaaa tatcttcagc accaattttc ttcttatctt tgttcgttgg
     1261 taataatgcc atttagttta atctcctttc caagaggtag gatatgattg gccattgagt
     1321 cagcatattt aggtgtataa tcaaatcctt caatatcacc atgttgttca tagtattcaa
     1381 ttctttcttc taattctgta aagatgtctg caaaaatagc ccaccatctt cgatctacat
     1441 aatcatttaa ttggatacca ttctcaccat acatttctaa ccgatccgag cctttaacaa
     1501 tgcttttaaa aatgtttgat tcaccatact tatcaattgt tttttgatag tttgcgtgtt
     1561 gagaggaacg tcgcactgag aaaagaacag gcaaaattcc aatgatttga agatgagact
     1621 cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat aaaatcttct gttccttcca
     1681 ttgcgtaccg ttttaattct tgcattgcaa taatataatc tgcagcagtt aagaatgcac
     1741 gaaccacatt atctgttgat ggtgggcagt cgataaagat ataatcgaaa ttttttctca
     1801 attcttctaa aggtttaatg aaggccaatg atctcttacg acgatcagga tatttttcgg
     1861 acaagtattc attaagttct tcagatccag gagagccagc cataatatat aaatgtggac
     1921 ttacttgagt aatacctgaa acccagtccc cttttttgat tgctgcagac atggatcgat
     1981 caaagctagt aatttgatac gttgatccaa tgttggaagt agtatttctt tgttggtcat
     2041 aatcaattag caacacgtct ttttcaaaaa gttgactaga aacaatcgct aataaatcat
     2101 tgtctgtagt cttaccaatg ccgccttttc tacctgtaag cattacaact tttcctagtt
     2161 tatctgatgt tttaaatgca gtccgtaagt tttccatagt tccctccaat ttcctatact
     2221 aaatatattt agtatacttc atatacttaa tatattcgat atatctaata tactccaaat
     2281 tgataagaat tcagacttta taaggaatat agtggatata ctaaatatat ttgctatatt
     2341 cagtatatca gatatattca aaatgtcaaa atatatttgt gcgttttgcg ttgacaattt
     2401 agagtgaaaa gaggtaaact taattctata gaaaccacag ataattcact atttacaaag
     2461 tagcaaaagc agtggtactc tttaaacaga aaaattacat aaaaaaaaga gacggaaata
     2521 attgatttta gcagaatcaa ttatttctga atcacatggt taggacatgt gttcacgctc
     2581 gtctcaagaa tatgtaagta gctaccaact acttatatta aaagttgatc ggataccttt
     2641 tccgattgcc tttattctac caaaaaaagg cgagcaactc aacagaagaa cgtttattta
     2701 gtgtacttaa attaaatatt cttaaacact gaagagagaa cacatactat ggctgagatt
     2761 aaagctaaac tgtgttctct ttttgtttga gaaaggacgt tacaatcgat gttgagcgaa
     2821 atttattcag ctattcttaa aaacggctta cgtacataca aatatcgaaa cagcaccatg
     2881 aaacctgcag gtcatattga ttccagtagg agtggtgcta tctttgcgta ccgtagcaag
     2941 caattaatga atgttgggcg tggtatggtc attacttcgg aagaagctat tttagagaac
     3001 gaaaagaaac taacgcattg gacacctaat acctatcgct ttggtactta tgcggatgat
     3061 tatcgaacag tggttaaagg tcattctgag aaaaacttaa gtcaaatcaa cacatttgta
     3121 gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta ttttagcctg tttagatcaa
     3181 gtgggctaca tgccaacgct aattttagaa tcagatcatg gttatcaagt ttattttgta
     3241 ctaaagacac caggctatgt cacaaagaaa agcaatttca aggtgatcga tgtagcaaaa
     3301 aaaatttcaa aaacaattcg tctacagttg gctgaaaaat tgtccggagt ggatctagga
     3361 tgtaaccact tcggaattgc tcgattccca caagaaaata atattgtttt taaagaaatg
     3421 aactatcagt attcttttgc tgactggatt caatggtcga tgaagcttga atcagatcaa
     3481 aaaacaaaag aagatcgaga acgaaaacta attgtctttc ctgagaaaaa agaaattcgt
     3541 caagtagatg aaccgtggtt cgatatgtta cttcataaag caaacatcgt cggcggagaa
     3601 ggacgtcttg gtcgaaataa tgtgattttc acgctttctt tagcttatta ctcttcaggt
     3661 tatgcattgg atacttgcga atacaatatg ttcacattca acgaacggtt acaggaaccg
     3721 ctatctgaag cagaattagt aaagattgta cgtagtgctt attctggtaa ttatcaagca
     3781 gcaagcagag aattcattat tgaattgtgc cagcaatggg tttcaccaac cttaaaaaat
     3841 aatgatctgt tcattcaacg acgtggctgg tggaaattta aaaagccacg agatcaaaga
     3901 aaatatagtc ataaaaatga atggaaagaa gatctattgg cttatctaag cgaaaaaagt
     3961 cttgctagtg agccttattt gaacctgtca aaaaaagaat tggcttcaca actaacgatg
     4021 cccaagcgaa cacttgataa gatcttaaat gagttacgag aggagaataa agtcttctac
     4081 aaggtaacac gaggaagaaa tggcggaatg atcattgctt cagtcaaaac gttgttcgcg
     4141 agggttattc aactaagtaa agaaaagaga ctagattatt caacaaggat cacagaggta
     4201 tttggactca acgagagctt agtagaagag acatttagac aattatcaac ccccttgaac
     4261 cctcttaaag aagtgaatct ttttgaagta gatacaggtt gaaaaataaa aggtgcaaat
     4321 tgccttatac aatatatcta tgatacttct gtaggtgttg ctccctttgc ctagctacgt
     4381 gtaggtggta tacttataac tgcgtatgca gtcttatgta tcatggagga agttgtatgt
     4441 cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt tatcattggt cgtttgggat
     4501 tatctgtgga agtgttctgt gagctttatg gttttaagca ggggactttg tcttcttggg
     4561 ttaccaggga aaaaacagtt gcttctttgc cgattgagtt tcttcatggg ttgtccttag
     4621 cttctgggga gactatggat caggtatatg attgtttgtg tgtgttagag caggagtata
     4681 ttgagtataa aatagctaac gagttaagaa aaaggaagaa gtatatccag tagtagtttt
     4741 tatttactta aaaaattggt tcattgagca acctatcgct gctaaagtag caaaggaaaa
     4801 cgttaaagga aagaatttag ggaagaaatc gaaatgaaat cggacgactg gattgaacac
     4861 acctggcttc ctctgaaaag aaatagcaaa cgaaagaaat tttgggtatt gcaattatgg
     4921 ccattaggaa aactttgtag ttacggggca cgacttattc ttgatttaac cgtttactcg
     4981 attggagtat ttggaacggc ttattacttg ataaatgatc ccaataggat tgcagcatca
     5041 gacattttta cagtgctaat gacgttgatt tttgtcatgc tattgtctat ttttactcaa
     5101 ttagtcatgc aatttaccag gcaaaaatta acagcaggaa aacttttcaa aagcctattc
     5161 gatttaattg ttgtaaatgc aattctagcg actttaataa aacaaattga acaatctgga
     5221 agaacaacaa aaggagtgaa ggatttttta ttgacgttta ttcactttgt aaatggacac
     5281 atgtatttgt ttgaagtggg tattattctc ttttttgtcg ggtatgtatc cacgaaactt
     5341 attaaacgtt caattgaaaa agaaattgaa attgacctga ttccgacact tcttggttat
     5401 gagtacaatg ttgattttgg aagatatgac gaattgatca atacggatat cgttcacacg
     5461 agtatccatg aatctactgt taagggcatc agatctattt taaaaaatgg ccaggaaatg
     5521 aaagcaatag atcgaagaaa agagatctac aatttttatg aagtcgaagc aagcctacga
     5581 tatggcatcg gaaaattgtg gttagatgga agtaatattc gtcaaaacaa accatacatt
     5641 ttttggaaca gtagtgattt agaacttcca atgaaagata aataattatg gagaatcgtt
     5701 tgaaaatgat tcctctatac aggattgtgc ttatagttgg catggagtta aaaaccttaa
     5761 agaaggagca gttattcaaa gtgaaagaac gtcataatca gttactatgt agtaaaacgg
     5821 gtacgatgaa cattgaatac cgaacagaat atttagaatt aacgaatcaa actgattttt
     5881 tgttgattcg aaagcaacaa gagcaagaaa aaaattaatg gctacttata gccaattgat
     5941 tgatgaatgg gaacaattca aggaatctaa aattccagaa cgtctctaaa agcctttatt
     6001 t
//
LOCUS       DBNICI010000152         5962 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.154, whole genome shotgun sequence.
ACCESSION   DBNICI010000152 DBNICI010000000
VERSION     DBNICI010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.154"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            729..1463
                     /locus_tag="AC1ZCC_002381"
     CDS             729..1463
                     /locus_tag="AC1ZCC_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIQ1630606.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1479..2648
                     /locus_tag="AC1ZCC_002382"
     CDS             1479..2648
                     /locus_tag="AC1ZCC_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIQ1630607.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2664..3659
                     /gene="lacD"
                     /locus_tag="AC1ZCC_002383"
     CDS             2664..3659
                     /gene="lacD"
                     /locus_tag="AC1ZCC_002383"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HIQ1630608.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3738..4664
                     /locus_tag="AC1ZCC_002384"
     CDS             3738..4664
                     /locus_tag="AC1ZCC_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HIQ1630609.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4683..5933
                     /locus_tag="AC1ZCC_002385"
     CDS             4683..5933
                     /locus_tag="AC1ZCC_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIQ1630610.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1999 a          972 c         1219 g         1772 t
ORIGIN      
        1 ggctcattgt caaggacaat cggaatagca tacggaaaga agttctgcac cttataaaat
       61 ttgtcaaaaa aactgttgcc attccaaagt ttgaaaaatg ctatttcgag gtagaaggat
      121 attatataag cacggttgac taaacgaagt gataataaaa aaagttattc aagaataaga
      181 aaaattatga tacgaattca gataaactgg agaatacaga gacctttaaa agggataaga
      241 aacaacataa attcctctta agagagaacg aaaggacaaa gccagcaact tgatacacga
      301 gctagtatta gaggaattta ttctcataga ctcccttttg aatacgtttc aactttaatt
      361 ttcatgttca taaatgattt gaaaccaaag caaatgcgtt gcacttttcc tccgtcttcc
      421 gaaaatcgtt caattcactg gtaagtagtc atttgatagt ttgaggcaaa ttgttgattg
      481 atgaagggta ggagctaaaa aaagatacca gttgaagaga gaaaaagaaa aaaatagagg
      541 gtgttcttga atgaaaaaag ttggaaaatt atttttcatt tccttttatt cctattaaag
      601 tcagtgatgt caatcatatt aacaagacta aaaatgaaaa taaaaaaaag attgttctta
      661 gtattgactc ataaaaaata ccactactat acttacattg tggttatagc cagttaggag
      721 agacatcgat gcaaaatacc tttcaatcaa aatcacttta ttatcagctg gtagaaatat
      781 tgaaacaacg aatcgaaaac agtatggtcg ctcatgacaa gctgccatca gaaagagagt
      841 tgaccagttt ttatggcgtt agtcgcacga cggtgcgact agcattgcaa gaactggaaa
      901 aaatgggcta catttatcga caacatggaa aaggaacctt tgtttcggaa ttaaggacaa
      961 atgcgactga tctagctggc gcatatagtt ttaccgaaca aatgcgggca atgggcaaac
     1021 atcctgaaac caagatattg gccttcaaga agattgaagc aactaaatat ttagcgagtc
     1081 atctgaatgt tcaactaggt gagcctttat ttcaattaga ccgattgcgc ttagctgatg
     1141 atttgccaat gatgatcgag aaatcctatt tgccagtgaa gctatttatg tccctttctc
     1201 aagaaattct atttctgaaa cctttatata atttgttttc agagaatttc aatcaaatcg
     1261 tacggttggc tgacgaggaa ttttatgcca gcattgtcca gaaagaagat agtgagttgc
     1321 taaatattcc agaaggatca ccagtgctcc atttagttcg ctcaacctat aatctcgaga
     1381 acgaagtgat tgaatttaca ttaagtgtag cgcgggctga taaatttcat tataagatca
     1441 ggcatctgcg ttccactaac tgaattggag gaaagaaaat gtttatgttc tcaaaagaag
     1501 aactacgaaa gctaggagca gaaaatacta cccgagaaat tcgacagcaa ccggaactgt
     1561 ggcatgagac gtttgaccgt tatcaagaaa cagttgcaga tttagatcgt ttgtttacgg
     1621 atgcgaaaca acatgctgga gagcgaccaa tccgagtcat ttttacaggg gccggaacct
     1681 cgcaatatgt gggagataca ctaattccgt ttcttactag tcaaggaaat acgaaccagt
     1741 ttatttttca aagtattggt tcgaccgata tcgttgctac gccaaaagaa tacctgattc
     1801 ctgatgagac aacgatcttg gtctcatttg cccgtagtgg aaatagtcca gaaagtctag
     1861 ccgcagttaa gatggccaat cagctggtca acaagatcta tcatatcgtg atcacctgtg
     1921 caccagacgg attattggct acacaaatgc ctacaaaaaa agaaaatcta gttttactaa
     1981 tgccaaacaa agccaatgac gctggttttg ctatgacaag cagtttcagc tgtatgacgc
     2041 tgactgcgct acttattttt gatcaagcaa gcaatcagca aaaagaaaga tatgttcatc
     2101 aattaagccg actaggagaa gaagtcatcc aacgagaatc agagatccaa gggtatctgg
     2161 catcagactt tcatcgcatc atttacttag gttcgggaag cttaggcgga ttgactagag
     2221 aagcgcaatt aaaagttctc gagttaactg ctggaaaaat cgcaaccgta tttgatacat
     2281 ctttaggatt cagacatggt cccaaatctt ttgtcaatga gcaaacattg gttttcctgt
     2341 ttgtcagcaa tgactcatat acgcagaaat acgatgtaga catgctggaa gaaatcaaaa
     2401 atgacggtat tgcccaacgc gttgttggaa ttggtcaaaa gggttcattg tttagtggtg
     2461 attcctttga attagttgca gattcgttac tgccggatgg ctatcttgct ttagcagata
     2521 tcatggtagc ccaaacgatc gcattacttt gttcggtgaa ggttggtaat acaccagata
     2581 caccatcgcc aagtggaaca gtcaatcgtg tggtcaaagg cgtaacgatt tatccatatg
     2641 aaaaatagga aagagtgtga agcatgttag aatggtcgga aaataaaaga caagcaatgg
     2701 aacgtctatc gacgccagaa ggcattatta ccgctttggc gatcgatcag cgaggcgcgc
     2761 tgaagaaaat gatgtcggct ttagggatgg aagcgacaaa ggaaaaaatt gagcagttta
     2821 aaacactaat ttcccaagaa ttgactcctt atgcctcagc aattttattg gatcctgaat
     2881 acggactgcc tgctgcacag aagcgtgcag cagcgagcgg tctgctgttg gcatatgaaa
     2941 aaactggtta tgatcaaacc gtacccggcc gcttgccgga tctgctgtcg gactgttcag
     3001 ttctgcgatt gaaagagtta ggtgcggatg cggtgaaatt tttactgtat tacgattatg
     3061 aagaagcaga ggaaatcaat cggcaaaagc aagtttttgt tgaacgaatt ggaagcgaat
     3121 gcatggctga aggactgcca ttttatttag agttattgtc atatgatgct aaaaatgtag
     3181 atacgaaatc gctttcctat gccaaagcaa aaccgcgcaa agtgatcgaa atgatgaaag
     3241 aattctcgaa aacccagtat aaagtagatg tcttaaaggt tgaagtacca gtaaacatgt
     3301 catttgttga aggaaatgca acaggggaga tcgcctatac acgtcaagaa gctgccgctt
     3361 attttaaaga acaaagcgat gcaacagatt tgccgtatat cttcttaagt gctggggtat
     3421 cagcggcatt attcatggag acattgcgtt ttgccaaaca tgctggggca cgattcaacg
     3481 gtgtactctg tggcagagca acttggaaag atgcagtcgc tcctttcgtt gaaaaaggag
     3541 aagcggcaac tttagaatgg ctgacagaac aagggacaca aaacattcgt caattgaatg
     3601 aagtgataag agaaactgcc atcccatggt atgagaaagt aagtgaaagc acttgttaat
     3661 gtttctaata tattagtagt gataagagat atgtgtgatt ataatttatc attattgaaa
     3721 ctgggaggat attttatatg aataaaagat tgttagatta ttcagcttca gattttttga
     3781 aagctaagcc gatggaatta aagcaggcaa ttttggcttc tgaaggtaga acaattatgg
     3841 cagaaaatgt gccaagtagc tgtccttaca tgggaaaatc agtgacaaat gcagaattgg
     3901 agagagctgc tggagctgat ttaattttgt ttaatgcact agatcttttt aatcctcaaa
     3961 tttcaggtgt acctttaact ctaaaagaga atccaataat ctggattaaa aaagctttag
     4021 gaagagctat tggtgtaaat ttagaacctg tagacaaaga agttttgatg agtgaaaatc
     4081 gaactgaaat ttcaagtggt agaatcgttt ctcctgagac tttaaaaaag gcaaatgaat
     4141 taggctttaa ttttatttgt ttaactggaa atccaggaac aggagtatct aatagaacta
     4201 ttgagaaagc aataacatta tccaaatcaa tttttaatgg attaattatc gctggaaaaa
     4261 tgcatggtgc tggtgttgat gaaccaatta tgaacttaga tatagcaaaa agatttatta
     4321 aaacaggtgt agatatttta ttagttcctg ctccatacac tgttccgtat ttcatggaaa
     4381 gtgatttgcg aggaatcgtt gattatataa aagattttaa tagaaataaa tctattgagg
     4441 aaaaagttct agtactaacg gctaatggaa caagccaaga ctcttctgac caagaaacta
     4501 ttaaaaaaat tgcattagca tcaaaagcat gtggagctga tattcaacac ataggggatt
     4561 ctttaaatgg tatttgtctt ccagaaaata tttttgcttt gggtcaagca attcggggat
     4621 atcgtcatca aattattatg ttaggaaaat ctaattatcg ttaattaagg aggaaatgaa
     4681 agatgatcga caaagttcaa aaaatagctg caaaaattag taatcaaaga catctactag
     4741 ctttaagaga cagcatggct gcagttatgc cattaatcat tattggttcc ttttttatgt
     4801 tattagcaaa tttcccgata gaaggattta caaaatggtt atctgaaatt ggagtacaat
     4861 cctttttgaa caaagcatca gattcaacat ttggtattat tggattagct gttacctact
     4921 ctttgggaca caatctagca aagacttatg gtgtagactc attatcagca ggattattat
     4981 ctttgagtgg atatgtttta cttacacctt tattggatgc agatggttct ataggatttc
     5041 caacaaaata ctttggtacc tcgggtcttt ttgttggtat tattgtagca cttgtatcta
     5101 cagaaatttt ccggtggttt gtacttaaaa atattacaat taaaatgccg gcaagcgttc
     5161 caccaaatgt aagccgagct ttcgttgcta ttattcctgg cttttttgtc gttctactat
     5221 ggtttattgt tgttgtaatt tgttataaac ttggaattga aaatgttcat gctcttattg
     5281 cagatacact cgcaaaacca ctatctttac tgacgaaaac tttaccagga attatattgg
     5341 ttatacttat tcaatgtttc ttttggatgt ttggtattca tggtgcacag gtaacggggc
     5401 caataattga accgttatta ttgcaaaact ctgatgtaaa tagaattgct tatcaagctg
     5461 gaaaagaact tcctaacatt ataacttatg aattcttata taactttgtt ttttctggtg
     5521 gtgcaggttg tttatttgca cttgccattt taacattttt cttttcaaag agtcaggaaa
     5581 ataaaacttt aggtaagtta tcaatcgctc cagtaagttt tcaagtagca gagccccttc
     5641 tatttgggtt tccaactata ttaaatataa aaatgttaat tccatttgta actgcaccag
     5701 tagtaacaac attaattact tatttttcaa tgagtatggg attagttgca aaacctgtag
     5761 gagcaacaat cccatggaca actccaccga taattgccgg atttttagct tctggtggac
     5821 gaataagtgg tgcagtgatt caagtaataa caattgcaat aaatgttttg atatactatc
     5881 cattctttaa attagatgat aatgccaagc taaaaagcga aaaaaatgat tagaactacc
     5941 tatatgtttc taactgtagt ta
//
LOCUS       DBNICI010000153         5924 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.155, whole genome shotgun sequence.
ACCESSION   DBNICI010000153 DBNICI010000000
VERSION     DBNICI010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.155"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(75..1169)
                     /locus_tag="AC1ZCC_002386"
     CDS             complement(75..1169)
                     /locus_tag="AC1ZCC_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HIQ1630611.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1358..2119
                     /locus_tag="AC1ZCC_002387"
     CDS             1358..2119
                     /locus_tag="AC1ZCC_002387"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIQ1630612.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2234..2674)
                     /gene="fabZ"
                     /locus_tag="AC1ZCC_002388"
     CDS             complement(2234..2674)
                     /gene="fabZ"
                     /locus_tag="AC1ZCC_002388"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HIQ1630613.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2894..3649
                     /gene="fabI"
                     /locus_tag="AC1ZCC_002389"
     CDS             2894..3649
                     /gene="fabI"
                     /locus_tag="AC1ZCC_002389"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HIQ1630614.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3759..4811)
                     /locus_tag="AC1ZCC_002390"
     CDS             complement(3759..4811)
                     /locus_tag="AC1ZCC_002390"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HIQ1630615.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            4937..5857
                     /locus_tag="AC1ZCC_002391"
     CDS             4937..5857
                     /locus_tag="AC1ZCC_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIQ1630616.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1806 a         1180 c         1063 g         1875 t
ORIGIN      
        1 ctataaaaaa acaaaataaa aaagcagaaa taactggcta tttctagcgg attatttctg
       61 caataactaa ttatctattt tttattttca attaatttaa agcttgacca tggtttcaaa
      121 aagtcaagga ggaataattc gtcatcggag attcttccta caacattgac acgtccgtcg
      181 tttttcatat ccttcaaggc gatttgtgtt tctcctttgt attgaccgta cccttcattg
      241 tcaatcagta catccccatg atgcatatct cgcgtattat gaggcgggaa atctttatct
      301 ttgtagtata cacgagtcat cgttgaacga aggatatatt ctgagcgatc ccctcgataa
      361 ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg taataccttc ttccgtatct
      421 actttcaatg ttggataatc cgcgttaaat gcttctgcca tttctttcaa ttcttcttca
      481 cttgcgtacg catttccaat agaaatatca tcaatcaatc ctgtaaggat taaatgcttc
      541 acttgtgtcg caatttcaag atctcggtgg tcttccagcg tgcacaagcc atcttgcgta
      601 ggccaagggc caaactcagc agattgcgaa ttgacgaatg ccatcgtatt caaattatat
      661 tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc ctaaaccaga gtatcgatga
      721 ggataaaaat tatgagaacc taataaatta tccgtgtttg gcgaataact catgatgttg
      781 tcaacgtaat tcgtgcccga actcatattg atctcgattt tgataccaaa aggattgcgc
      841 gtcatttttg cttcttctgc tcctgtgaat ccgatatcta agcggacccc gtaggcaccc
      901 atcttatgga aaaaagataa gtcatcgtaa gaaatctcca actgttcaaa taacgctgga
      961 ttgatatcaa ccattacttc catgcctagg ctattcgcgt aatcaactac ttctttaaat
     1021 tctgaaagaa ctttttcttt atcatcattg atctgcagca aactggtaaa aacccgttta
     1081 tacccatact tatgagctaa atccaagtac gctttgtcct tttcaaatgt tgaccgttct
     1141 ggataaatcg agattcctaa ttttcccata ttaccatttc ctcctgcatc ttttcgtttt
     1201 tgtatccact tacattatag caaacacaaa gtaaatggtc tacacctttt ctttttcttt
     1261 taaaataaat gataaattcc aataatttaa gtaggtattt tccctctatt ttgttatcat
     1321 taaagataac aaaatagtaa attggaaagg aacagttatg aagaaaaata aagttatttt
     1381 aagtattagt ttagcagcag cattgctgtt tgctgctggt tgttctgcaa acaacacgac
     1441 ttcttctagt tcgtctacag aaagttcaaa aagtacatct actgcggaaa gcacgactgc
     1501 cagtagcgtc gatttgaacg cattggaatt accacaattg acgaaagatg tcgccgaaaa
     1561 tgaagatctg gtcgaaatgg tgacgaataa aggcacaatc aaaatcaaat tatttcccga
     1621 acacgcacca aaagcggttg aaaactttat gacacatgca aaagacggtt actacaacgg
     1681 actgactttc catcgcgtca tcaaagattt catgatccaa ggtggcgatc ctaaaggcga
     1741 tggcactgga ggggaaagca tttggggaga aggatttgaa acagagatca acaaccacct
     1801 ttataatata agaggtgcgt tatctatggc tcgctcacaa gatcctaaca gtaacggcag
     1861 ccagttcttt atcgttcaaa atacagatga tatgcatgat ggtcttttaa aagacgacta
     1921 tcctcaagca atcatcgacg cttataaaaa cggcggttat ccttcactag atggccatta
     1981 tactgtcttc ggccaagtga ttgaaggaat ggatgtcgtg gatgctattg caaatatcga
     2041 aacagatagt tcagacaaac caaaagaaga agtaaaaatc gataaaatca atatactgca
     2101 agaagcaaaa caaaaataaa aaaataacgt ctgaatcctg catagatgac aacgtatgct
     2161 aggattcagg cgtcttcttt ttacataaaa agcaagtaag cttccttttg ttttgctata
     2221 tcaggtttta tagtcaagca ttttggttga cgatgaacgt gaactcgcac tcacagactt
     2281 ttttatcatc tacatatgct gcagataacg ctgtaccgac tgatcccttc attttggtga
     2341 tttcaaaatg acatttcagc acatctcctg gaacaacttt ttgacggaat ttggccttat
     2401 tgatcccacc gatataagct ctttttccta gaaactcttc tgattttagg ataagtatcg
     2461 aacctgcttg tgccagtgtt tctagaatca acgcacccgg catcactgga ttgtcaggaa
     2521 agtgaccttg gaaaaaatct tcattgattg ttacattttt tgtcgcaacg atcttttttc
     2581 ctggttctaa agtatctaca taatcaatat aacaaatagg ataacgatta ggaatcaatt
     2641 ccattacttc agtagcagat aatacgtaag acataattaa actccttctt tcaggcaatt
     2701 gccattttca ttactttgac tatcaaagta tcatattcct aaatatttag caatcttttt
     2761 ttaatttaag aaaaaatagt ttgacaatca aaacattttg actaataatt aagaaatcaa
     2821 gaagttattt tagctattat tctctatttt tttacgacta tcaaattttt atcagaaaca
     2881 aaggagagtc attatgtcat ttctagaagg aaaaaaaatc gtcattatgg gcgtcgctaa
     2941 caaaaaaagc attgcttggg gttgtgctga agcaatgttg aaacaagggg cagaaattat
     3001 ctatacttac caaaatgaac gaatgaaaaa atcattagtc aaattggtcg gcgaagaagc
     3061 attcactgta gaatgtgatg tttctagcga cgaaagcatc gaaaaagcaa tgaatgccat
     3121 cggtgaatat gctggggaga tccacggcct tgtccatgca gttgcttacg ccaacaaaga
     3181 agaattgtcc ggaaatgtgt ctgatatttc cagagacggc tatcaattag ctcaagatat
     3241 cagcagctac tctttaattg ctgtatcaaa atacgcacaa ccgtttcttg caccaaacag
     3301 cgggatcgtt agcatgactt atctaggttc agaacgcgca atcccgaatt ataacatgat
     3361 gggtattgcg aaagcttctc ttgaagctgc tatccgttat ttggcagctg agttctcgcc
     3421 taaaggaatt cgggtcaatg gaatctcagc tggggcaatc aaaacattag ctgtcacagg
     3481 tgttaaagac tatcaaaaac tgatccagtt atctgaatct cgtacgcctg atcagcaagg
     3541 tgtgacgatc gaagaagtcg gtaatgcttg tgctttctta atcagcccac tttccagcgg
     3601 aatcatcggt gatattatct acgttgacaa aggtgtccat ctttcataaa cttttgttgc
     3661 attctttaaa gtaaaacata taaaaatcag gaaaaaactt tttaaagtct cttcctgatt
     3721 ttttcttttt acgttttttt caactcatta ctttgaaatt attccacgac ttcaatccag
     3781 ccttctggtg cttctatgtc tccaaattga atacctgtca attcatcata caaccgtttt
     3841 gttactggtc ctacttccgt ttcactataa aatacatgaa gattgttatt gtgataaatc
     3901 ccaccgattg gagaaataac agccgctgta ccacatgcac cagcttctgc aaattgatct
     3961 agatcatcaa tgaaaatatc tccttcaatt gcttgcagtc ctagtcgttc ttcagctaac
     4021 tgcaacaagg aatatttcgt gatacttggt aagatagagg gcgattgcgg agtgatgaat
     4081 tttccgtctt ttgtaattcc aaagaaattt gctgcaccta cttcttcgat tttcgtatgt
     4141 gtggctggat caagataaac acaatctgag taatccagtt cttttgcttc tgttcctgga
     4201 agtaaactcg ctgcgtaatt tccgccgact tttgctgcac cagtcccctt aggagcagca
     4261 cgatcgtaat cagatacaat aaaattcgtt ggttttagtc cacctttgaa atacgctcct
     4321 actggcgtac agaagacgac aaataaatat tctggagctg gatggacacc aatgttttca
     4381 ccgaccccaa tcagtaaagg gcgcaagtat aatgtagcgc ctgttccata gggcggcaca
     4441 aaatcttcat ttgcttttac cacttgcttg acagcatcaa cgaacatctt ttcagggacc
     4501 attggcatca gcaatcgtct agcactttga ttcatccgtc gactattttg ttcaggtcga
     4561 aagagattga ccttgccgtc ttttcgacga taagctttta gaccttcaaa acattgctga
     4621 ccataatgaa gcgctggaga gccttcatga atcgtcaggt aattatcttc tgtcaattct
     4681 ccttcttccc attgtccgtc tttccatttg gcaataaaac gccaaggagt tttgatataa
     4741 gaaaaaccta gattgttcca atcgatttgc atatacttac caccttcgtt attttctcca
     4801 cattttatca ttatccatat tatttgtcat tttattttca gcttttttcc ttcattatag
     4861 ttatttttgc tatttccttt tttgaaaact tcttggtaca atacagaaag aatcaaattt
     4921 tagaaaagag gattttatgt tttcattatt agcaactgcc ggtacgacag actcttctgg
     4981 cgagacattg acagatcaag ctgtcagcaa agtaaatgcg tttcagcggt tttgggacgg
     5041 gattgattgg gacgccattt tggctgcctt tatccaaaaa agtttatatc tgattttttt
     5101 gatcgtcctt ttttggattc ttcaccgtgt gggaaaatat ctgattgata agtcttttaa
     5161 tcagtattct aaaaaagcca ttctaaacga gagccgctta aagactttac atagtctttt
     5221 aaatactatt tttcattata cccttggttt cttttttatc tatgcaattt tatctgctat
     5281 cggtgtgccg atcggatcac ttttggctgg tgccggaatt gcaggtgtgg caatcggtct
     5341 tggtgcccaa gggttcatga gtgatgttat taccggattt tttattatca tggaacaaca
     5401 gatggatgtt ggggactaca tcaagctagc aaacttaacg attgaaggaa ccgtcgtatc
     5461 tgttggtatc cgtacccttc agctgaaagc aacggatggt actgtccatt tcatcccgaa
     5521 tcgaaacatc accacgatca gcaatctctc tcgtgcaaat atgcaagtac ttttagatat
     5581 tcggattgtt cctgaagaag gatatgacaa aatctatgag atcattgatc gagtaaatcg
     5641 tacattagct gaaaaatacc aagaagagct ccaaactgaa ccttctatct ttggattagt
     5701 ggatcttgga aacagcaatt ttgcgattcg tacggtctgc tacgcattga acggaaaaca
     5761 atatgcctta aaagaagagt tcttgagctc ctatgtcaaa gaattgacag cttctggttt
     5821 cacgattccg aacagtccaa ttgctgtcgg aaaataattt ggacaaacga tgcaacgaat
     5881 ctcactaaaa agagcgtggg acaaaagtaa agaacacttt tgtc
//
LOCUS       DBNICI010000154         5834 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.157, whole genome shotgun sequence.
ACCESSION   DBNICI010000154 DBNICI010000000
VERSION     DBNICI010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.157"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            158..730
                     /locus_tag="AC1ZCC_002392"
     CDS             158..730
                     /locus_tag="AC1ZCC_002392"
                     /EC_number="1.16.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HIQ1630617.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            875..1579
                     /locus_tag="AC1ZCC_002393"
     CDS             875..1579
                     /locus_tag="AC1ZCC_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIQ1630618.1"
                     /translation="MTKHTHHEIGDHAACIRLVPIFNHLEDSAMNYIAEKAHTKQYQK
                     GEFLFRSQEKEDTLYIINRGKIRIYRLADSGKEQLVRILNPGDFTGEWTLFNPDAVHE
                     EYAEATRDTVVCMIQQKDVQDFLEQYPAISLKLLAEMSKRLESSERQTTQVAVESVIT
                     RLVLFLAENVEPEMGNSPTITLPMAKKDIASYLGTTPETISRKFGELEDEGLIQQLSG
                     KRIKIQDLDDLLLYSE"
     gene            1807..2145
                     /locus_tag="AC1ZCC_002394"
     CDS             1807..2145
                     /locus_tag="AC1ZCC_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630619.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            2130..2984
                     /gene="lgt"
                     /locus_tag="AC1ZCC_002395"
     CDS             2130..2984
                     /gene="lgt"
                     /locus_tag="AC1ZCC_002395"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIQ1630620.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3131..3364
                     /locus_tag="AC1ZCC_002396"
     CDS             3131..3364
                     /locus_tag="AC1ZCC_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIQ1630621.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            3562..5466
                     /locus_tag="AC1ZCC_002397"
     CDS             3562..5466
                     /locus_tag="AC1ZCC_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIQ1630622.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISIGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
BASE COUNT         1893 a          967 c         1326 g         1648 t
ORIGIN      
        1 aatttaatag agaagcctgg cgtctattaa gaaataattt tcttgatgag cgtcaatttt
       61 tttaacggaa atctatccta tactaaattc agtaaagaaa aaggaggaat caaagaaagc
      121 tattttaaac aaaccaaaat tataggagga ataaatcatg actgaaaata aagctactca
      181 agaaagaaca ccccaagaaa gattgcaaga agaacaagaa cacaaggaac atgttcatca
      241 tacgaaaatt aatgcagcgg ccattgcgga tcatctttta ggcaacatgc atacattaca
      301 tgtgaaactg caccaatatc actggtatgt aaaaggagcc aatttcttta cgttgcatga
      361 aaaatttgaa gagttgtata acgaaaatga aaaatggttc gacaaacttg cggagcgtct
      421 aattacttct gggcataaac ctgcttcgac aacggttgaa tttgaaaaat attcgatgct
      481 ttcggaagat gcagttaata aatacgctaa agcagaagac atggttgaaa atattatcga
      541 ggacttcaga agcactcgtg acctaactat tcgcgcgatt cgtttagctc aagacgaggg
      601 tgacgatgct ttagaagata cattgattgc ttttaaaaat gacttagaca agaacatttg
      661 gatgctgcaa gcgttcattg gtaaagaagc attagaagat gatgacgctc ttgatgagga
      721 tgaagattaa ggtaaaagta gaagctgatt tagggattgg aattctttcc agtccttttt
      781 ccattttctt gatgtgcatc aatttttttg tagtaagaga cttatataat aaaagcagcg
      841 aatataaaca aaataaataa agggggagaa tgaattgact aaacataccc atcatgaaat
      901 tggcgaccac gcagcctgta tccgcttggt tccaatattc aatcacttag aggatagcgc
      961 gatgaattac attgctgaaa aagcccacac gaaacagtat caaaaaggag agtttctgtt
     1021 tcgttcacaa gaaaaagagg atactttata tattatcaat cgtgggaaaa tccgtattta
     1081 tcgattggca gactctggca aagagcagct agtgcggatc ttgaaccctg gtgatttcac
     1141 cggagaatgg accttgttca acccggatgc agtacacgag gaatatgccg aagcaacccg
     1201 agataccgtt gtctgtatga ttcagcaaaa agatgtccaa gattttctgg aacaataccc
     1261 agctatttct ttaaaactat tggcggaaat gtctaaacgg ttagaaagtt cagaacgtca
     1321 aacgacacaa gtagccgtgg agagtgtcat tacccgttta gttttgtttt tggcagaaaa
     1381 tgtggaacct gaaatgggca actctcccac catcaccctg ccgatggcaa aaaaggacat
     1441 tgcttcctat ttaggaacga cacctgagac catcagtcgc aaatttggag aattggagga
     1501 cgaaggattg attcaacagc tgtctgggaa aaggatcaag attcaggatt tagatgattt
     1561 attgctttac agcgaataat cggacacact tttttctaca cgttggtgtt ctgtcgctct
     1621 tcattaacaa cgggacagaa ctcagtgctt ttgcttccat ctgtccgttt ttggcttttt
     1681 taagacagaa cgaccttcat tcattggaaa aataaaagca caaacatgat aaccctcaag
     1741 tttttttgtt ttatttttct ttaagatagg gttgtcgaac aaaacgtaaa aataagtagg
     1801 gtgaatgtgc attgtttttg ggtgatagtt gccatcacgt tgtcagcagc gggggcattc
     1861 tttgtcgtcc ctttcaccaa gaacacgatt ggcggcgaag caaaaacgtc taacgttgtt
     1921 gaggcagata ggttagctat ggaaaaagaa gaggaagttg taaaaggagc cggcgtaatc
     1981 ggacacggaa aagtggcgta ttacgaagcg agtgaccagg gagataaaag tgtattcact
     2041 gtttggtttc catggagctt agccggaatc ttgttcagca tcaccatgac tagaatgacg
     2101 cgaaaaattg aagaaatgga gcaaatagaa tgggattcgt attaggcgtt ttgaatcccc
     2161 atgtggtgga gattggttct gttgtgattc attggtatgg agtcattatt gcagcgggca
     2221 tattagcggc tctccagttg agcaccaagg aagcaaagaa aaaaggactg gaagaggata
     2281 ccattatcga tatggcctta tgggcaattc cgattggact cctcggcgct cggctttatt
     2341 atgtgttatt tgaacttggt tattatctgc aaaatccagg acaaatcctt gcgatttgga
     2401 atggcggcat tgcgatttat gggggattga ttgcaggagg gggtacgcta tattggtatg
     2461 caaagaaaaa aggtacttct ttggcactcg ttcttgatat tttagcgccc aatgtattgt
     2521 tagcccagtc cattggtcgt tggggcaatt ttatgaatca ggaagctcat ggtgggccgg
     2581 ttactcgtca attcttggaa aatctttatt tacctgaatt tatcattgaa caaatgaata
     2641 tgaatggtac ctactaccat ccgacttttt tatacgagtc attgtggagt ttgttgggct
     2701 ttgtcctcat cgtcactata cggaaccgaa aacagctttt gcgtcaagga gaagtcgctt
     2761 tgagttacgt tttgtggtac tcagtgggtc ggttcttcat tgaaggtatg cgaacggaca
     2821 gtttatggat tggcgagtgg atccgcgttt cgcaagcctt gtctctggcc ctgtttattg
     2881 gcgcgattgt agtatggttt atacgcagac aagattatcc accaatttct tattattctg
     2941 atggcaataa ggggcagaaa aagactatta agatggtgaa ttgagcaaaa agataaaaag
     3001 aagtgcaaaa cttgatatgc gtcaattttc gtttgctaaa agtgagctat actacagttg
     3061 taaagaggaa aaggcagtgt tcattaatct gctgccgata aaataaaaac acataaagga
     3121 gataggaatt atggaaaaag ctatattgca attagaaacg ttgtcttgtc caagttgtat
     3181 gcaaaaaatt gaaggtgccg tgaaatcagt taacggtatt gataaagaca gcattaaagt
     3241 attattcaac tccagcaaag tcaaaaccaa ttttgattca gctgtcactt cgattgaaga
     3301 gattcaaaaa gctatcgaaa acgtaggcta tccggttctt aaagcaaaag tcaaggccgt
     3361 ttaacagatt aaatgaacta ggcaattctg gaagaaaagc tatttttcca ggattgcttt
     3421 ttttgttgca atccgattcg aggtgcttag aaacgataaa agagaacttt ctaaacttga
     3481 tgtcagtcaa ttaattactt aggcaattat cttatactgt gtttatcaat aaaagagaaa
     3541 ttaaatttgg agggaaaaag catgcaacaa tatatattaa gcaagaaaaa tgttatcacc
     3601 gtcatcagtg gattgttaat cgtactcggt ttcttcagtc actttgtttt agaaaacgta
     3661 ggactttcag agtggtcttt gatcatcgcc tctgtctttg ggattacgcc aattgccatt
     3721 caagcgtttc aagcaatgaa agtcaaagtc atcagtattg atgtcttagt cagtattgca
     3781 gcgattggtg cgctttttat tcaaaattat gaagaatcgg ctattgtcac gttcttattc
     3841 ttattcggac actacttgga acaacgaaca ttgaaccaaa cgcgatctgc tatcaaagaa
     3901 ttgactgaaa tggcacccga aagcgccttg aaacaaatgg ataatggcga atttgaagaa
     3961 gtagaagtgg atgatataga tgaaggggat atcttgctcg ttaaaacggg tgcgaaagtt
     4021 ccagtagatg gcatagtcct tacgggtgaa gggcatatca atgaagctag cattacaggt
     4081 gaagctattc cggtcaacaa gctagctgat gcagaagttt ttgcaggaac cattttagaa
     4141 aacggaacga ttcaaatcag agctgaccga gttggtgagg acaccacatt tggaaaaatt
     4201 attgaactcg tggaagaagc acaagattct aaatccgaag cggaacggtt cattgatcgc
     4261 ttttctaaat actacacacc agctgtcttg gttctggcaa ttgttgtatg ggcgttcagt
     4321 caaaatattg agttagcaat caccatcttg gttctaggtt gcccaggcgc attagttatc
     4381 ggagtgcctg tctcaaacgt tgccggtatt gggaatggtg ctcgtaacgg tgttctttta
     4441 aaagggagtg aagtcattca agacttcagt aatgtggata caattgtatt tgataagaca
     4501 ggtactttaa ctgtcggaaa cccaaccgtt gcagcgactg aactgtatga aaaagatcct
     4561 gctgaaacgc ttggctatct ggccagtgtg gaacgggaat cagatcatcc attagcaaaa
     4621 gcggtcttaa atcaaattgg agaaacaaac ttttatcctg ttgaaggaac tgaagttgtg
     4681 aaaggcggcg gaatcgtttc aagtgtagct ggacatagag tggctgttgg gaatgtggcc
     4741 ttgatggaaa aagaaaatgt cactctcagc aaaaaagtgc aaaaagatgt caaacggttt
     4801 gaacaacaag ggaactctct cgttttgaca gcggtcgacg gtgaattaaa agtactgatg
     4861 ggcattcgcg atcaagtccg tccgggtgtg aaagctaatt tgcaggaatt aaaagacttg
     4921 ggcgtgaaaa atctagtcgt tctttcagga gataaccaag gaaccgttga tgtggtcgcc
     4981 ggcgaacttg gtttgaccga agctcacggc tacatgttgc cggaagacaa atcggcttat
     5041 atcggaaaac ttcaacaacg tggtcaaatt gtagcctttg ttggagatgg cgttaacgat
     5101 agtccgtcct tagctttagc agacattgga atcgcaatgg gaagcggaac ggacgtagcg
     5161 attgaaacat ccgatgtcgt tttaatgaac tcgaacttca gcaacttgcc tcacgcatta
     5221 ggattagtaa aagccaccgc aaataatatg aaacaaaata tcgtgatttc aattggagta
     5281 gtgttggtct tgttgaccag cgtcttcttc agcgaatgga tgaatatgtc tatcggaatg
     5341 ttggttcatg aaggtagtat cttggtggta attttcaatg gcatgagatt aatgaagtat
     5401 aagttgaaag gtcgaaaaga acaaaaagaa gtatcagcac ctgcagagaa agtaaaaata
     5461 tcttaagaat aaattagaag aggctgagac aatagtccat ccttgaaatg acaaaagcgg
     5521 aaactcagat ttttaaatct gggattttcc gcttttttga gttcttgaaa aaaaataaga
     5581 gtatagagta tgttctttat aaaattaatt ttcgtgtaaa tgattaaaaa atttaataat
     5641 aaaatttaaa ttcctattaa gttgtaacac atgaaattgc ttataatagt gtattgaaat
     5701 taaggtagat tgacaaacga gtgatcaaat agatatacta ccttcaaacg accgtttgaa
     5761 tgtgaggtga gatgaatgga aaaagtttgc caagtgacta tcgtacataa agaaaaagta
     5821 aatgtaataa aaaa
//
LOCUS       DBNICI010000155         5821 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.158, whole genome shotgun sequence.
ACCESSION   DBNICI010000155 DBNICI010000000
VERSION     DBNICI010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.158"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(6..1208)
                     /locus_tag="AC1ZCC_002398"
     CDS             complement(6..1208)
                     /locus_tag="AC1ZCC_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIQ1630623.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            1325..1840
                     /locus_tag="AC1ZCC_002399"
     CDS             1325..1840
                     /locus_tag="AC1ZCC_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIQ1630624.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(2039..2485)
                     /locus_tag="AC1ZCC_002400"
     CDS             complement(2039..2485)
                     /locus_tag="AC1ZCC_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HIQ1630625.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2532..4010)
                     /gene="msr(C)"
                     /locus_tag="AC1ZCC_002401"
     CDS             complement(2532..4010)
                     /gene="msr(C)"
                     /locus_tag="AC1ZCC_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HIQ1630626.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(4364..4993)
                     /locus_tag="AC1ZCC_002402"
     CDS             complement(4364..4993)
                     /locus_tag="AC1ZCC_002402"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIQ1630627.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(4977..5441)
                     /locus_tag="AC1ZCC_002403"
     CDS             complement(4977..5441)
                     /locus_tag="AC1ZCC_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIQ1630628.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
BASE COUNT         1824 a         1227 c          970 g         1800 t
ORIGIN      
        1 atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat acaacataca
       61 ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat atgttcacca
      121 atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa actatagctg
      181 gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt caacagtgta
      241 ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag gactggcata
      301 gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc tgctaaaatt
      361 gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga agcaatagcc
      421 agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata gccaatcaat
      481 aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa tgcgattagt
      541 ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag gcctactttt
      601 ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc gcaaaacatc
      661 aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac cccaccgact
      721 agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat tgctttttct
      781 gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa taaagcattc
      841 ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga taatccgcca
      901 agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt caaaccgatc
      961 cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat gtaactagta
     1021 aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt ttttaagatt
     1081 gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac aactagcgct
     1141 gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg attctccaat
     1201 atttccatcc ctttctctct attagcatca tacatgattt attttacatg tcaatatgca
     1261 catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga gaggtaggat
     1321 ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac cacgttctgg
     1381 gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc agatctatac
     1441 aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag acgagcactc
     1501 tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg aatggatcaa
     1561 agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct atgctttttc
     1621 gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac agctgaacaa
     1681 aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga aacaagaatt
     1741 tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac tccaatggtg
     1801 tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca gaacctttct
     1861 agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt tggaaaagaa
     1921 aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt ctagaactgc
     1981 attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca gtgttccatc
     2041 attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg atgatcaact
     2101 gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca gagaataatt
     2161 gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc accattccta
     2221 aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga tttactggta
     2281 gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa atgaaagcca
     2341 ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct tgatgcagcc
     2401 ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt gtaaaaatta
     2461 ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc acgaaaatca
     2521 tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc ttttatttca
     2581 taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa caaaatcgtt
     2641 cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc aataaaatta
     2701 gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc aatcgtcaga
     2761 cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc ctgagcaaac
     2821 cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg tctcatgaca
     2881 aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata tacttgaaag
     2941 cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc taataaggtt
     3001 gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga aaactgaaaa
     3061 tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg tcccatgatg
     3121 ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg tttcatctgc
     3181 tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt cgcattcttt
     3241 tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc cagtctgatt
     3301 tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt tttcttttct
     3361 tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc tcgtagctga
     3421 gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga aaactctcgg
     3481 atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa atagcgatca
     3541 tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag tcgatcaata
     3601 ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc ataagaagaa
     3661 agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg ctctgaagtt
     3721 tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt tgcttcagcg
     3781 agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt cccatgatcg
     3841 ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc attttctcct
     3901 atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag ttctagctgg
     3961 ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat atgttttaca
     4021 ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa aatctgtttg
     4081 caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac taaatcacca
     4141 aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat actccatcca
     4201 tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa aagttcgaaa
     4261 cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt accacacacg
     4321 attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg aacatagtga
     4381 tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt cctattgata
     4441 aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc aactgatctg
     4501 cttcgaatcc accaattgga tttgtttcat agccatgatc acgcgcaact aacatgaatt
     4561 gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct cttgaaaaac
     4621 tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt ggcatcttcc
     4681 ctgattcgta agcttggttg tagatttctt cgccatattc gtaacattgc atatcgccaa
     4741 aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg atcaaaggac
     4801 gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt ttcatattga
     4861 cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg tgtgggattt
     4921 tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt gaaaaatcat
     4981 ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca aagttgggta
     5041 ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa agccggggat
     5101 agcacggaac ctgtatcttt atggtccatt tcacaatgct tgattttctt ctgggctttt
     5161 tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg cgaaacatag
     5221 ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat ctgcaacttt
     5281 tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat gatctcgtat
     5341 ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg atcggctaat
     5401 ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc ctttctaata
     5461 attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa agtcaaagaa
     5521 tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag aaattttttg
     5581 tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat tattgattct
     5641 tatcaaacta tagaagatag ctcttctgca gtagataatt ttacaccgcc aaaaatttcc
     5701 gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta tgcttaaaca
     5761 gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac ggtgttcaaa
     5821 a
//
LOCUS       DBNICI010000156         5816 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.159, whole genome shotgun sequence.
ACCESSION   DBNICI010000156 DBNICI010000000
VERSION     DBNICI010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.159"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(13..837)
                     /gene="nadE"
                     /locus_tag="AC1ZCC_002404"
     CDS             complement(13..837)
                     /gene="nadE"
                     /locus_tag="AC1ZCC_002404"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HIQ1630629.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(849..2321)
                     /locus_tag="AC1ZCC_002405"
     CDS             complement(849..2321)
                     /locus_tag="AC1ZCC_002405"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HIQ1630630.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(2958..3320)
                     /locus_tag="AC1ZCC_002406"
     CDS             complement(2958..3320)
                     /locus_tag="AC1ZCC_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HIQ1630631.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            3543..4295
                     /locus_tag="AC1ZCC_002407"
     CDS             3543..4295
                     /locus_tag="AC1ZCC_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIQ1630632.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(4435..5358)
                     /locus_tag="AC1ZCC_002408"
     CDS             complement(4435..5358)
                     /locus_tag="AC1ZCC_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIQ1630633.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(5373..5645)
                     /locus_tag="AC1ZCC_002409"
     CDS             complement(5373..5645)
                     /locus_tag="AC1ZCC_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630634.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         1738 a         1175 c          964 g         1939 t
ORIGIN      
        1 tttgctttat tgttatttcc agaaatcatc caaaacggaa attggcaaat gtcgtttatg
       61 ttgtgtcttg ttccaccatg cttcgatttt ttcttgtgct tgagcgggaa cttcttttcc
      121 ttctagataa tcatcaattt cattatatgt gacacctagt gcgacttcat ctgcaatcat
      181 tggtttccca tcttcaagat ctgcagtcgg aatttttgtg tataattttt ccggtgcatt
      241 caatgcttga agcaattgtt tcccttgtcg tttgtccaaa cggaataatg gcaaaatgtc
      301 ggctccacca tcaccaaatt tcgtgaagaa tccagtaatg ttttctgcag catgatctgt
      361 ccctaatacg gctcctgctc tttctccagc tacggcatat tgcgtaatca tacgttgacg
      421 tgctttgata tttcctttgt taaaatcgct gattcccaca cctgcttcgc ttaatacttg
      481 tacttgtgca tcaacagctg gtttgatatt tactcgcaga ctaacatcgg gctggataaa
      541 gtcaagagca gcttttgcat cttcttcatc tgcttgttca ccataaggaa gacgaacagc
      601 gataaattga tagttttcat cttttgtttc ttcacgcatt tcttccatcg ttagttgcgc
      661 caaacggcct gctaaagtag aatcttggcc gccgctgata cctagtacaa aagttttaag
      721 aaacggatat tttttcaaat atgctttcat aaagtcaata cttttacgta tttcttcttt
      781 tggatcaatt gttggtttga cacctaattc attgataatt tctgtctgca ggctcattta
      841 ttctcctcct atgcttctgt atgaagttct gctacgcttt ttttcattcg ttccatgata
      901 gccattttat ggttccagca ttcagtagac agatcgactg ggtatttttg cggattcaag
      961 tctcgtttat actcttccca tagtgaatct agattttctt ttgtatattc tttgatttcg
     1021 tttagtgcag gtaattcgta aactcttttt ccttcgataa agatatcttg taaaactggg
     1081 cgagctgtaa agtctcgaac agttttattg ataaacgtat gcactggatg gaacatgaag
     1141 atagcttctt cttctcgagg gtcttcatcc cataatgtca cgtagtctcc ttcagatttc
     1201 ccatcaaaat ttcgagtgat gcgccacact tgttttttcc ctggagtagt tactttttct
     1261 gcattgcttg ataacttgat cgtatccatc atttttcctt catcatcttc gatcgatact
     1321 agcttgaata ctgcaccaag ggctggctga tcataggctg tgatcaattt tgtcccaaca
     1381 ccccatacat caattttagc tttttgcatc tttaaattca aaatcgttgc ttcatccaag
     1441 tcattcgaag catagatttt tgcttccgta aatccggctg catctaattg ttcccgaaca
     1501 cgcttagata tataggccat atctccactg tcgatgcgta cgccttggaa gttgattttg
     1561 tcacctaatt ctttcgccac gcgaatcgca cttggcacac ctgatttcaa cgtatcataa
     1621 gtgtcgacta agaacacgca gtctttatgg gttttagcgt aggccataaa tgcttcataa
     1681 tcatttccat aagattggac taacgaatga gcatgggtcc cgctagctgg tatcccaaat
     1741 atcttcccag cacgtacatt gcttgttgca tctgctccgc cgatatatgc cgctctagtt
     1801 ccccagactg ctgcatccag ttcttgtgcc cgtctagttc caaattccaa tagtggatct
     1861 tctccgatga cagatttgat acgagctgct tttgtcgcga tcaacgtttg gaaattgacc
     1921 atattcaaca gtgctgtttc tactaattgg cagtgcgcta aaggcccttc cacttggatt
     1981 aatggttcat ttgcaaaaac tagttctcct tcacgagcag aacgaactgt tgctttaaat
     2041 tcaaaatttt gtaaatactc caaaaaacct tcaggataga cttctaatcc acgcaagtaa
     2101 gcaatatcgg aatcattgaa cgtcagattc tctaaataat tgactaaccg ttccaatcct
     2161 gcaaaaaccg cgtatccgtg gttaaaaggc atttctcgaa agtaacattc aaaaacagca
     2221 tgtaaatcag ctcgacctaa ttcccagtat gtctgcatca tattgatttg atatagatcc
     2281 gtatgcagcg tcaaactgtc gtctgggtaa gtattattca tcttgtatta ctcctcttgg
     2341 caagttttca tttcctaccc attataacaa aaaaaattaa taggaaaaga aatcttccat
     2401 tttttctgaa aaagcacatg ttttacggat atttcttgct aaaaatgaac tccatctgct
     2461 aaaactatcc tttcttattg gtatttaacc taaacgagaa agaatactta ttttattcaa
     2521 tgtatccgag acacgaagaa attagcgttt tctatttttc ttacttgata aaataagtag
     2581 attttcctac tacaatcgta gccgttaaga taccgatgat tgaacaaata taaccaaatg
     2641 tctctacaac ataatcaatg gcaaaagttt ttgaagcaat aatacgtctt tccttttttg
     2701 cttcttatcc ccaaagaaac ggatataact tattatgtat tgaatttttt tggaaaggcg
     2761 aattatgaca agatatacct tgcgttgaaa aaactcgcgg aaaaaatatc acacgttttt
     2821 aaaaagatac aaagtaagaa agcagctgtc aatagaatgg aaacaaaaat ggcgagacat
     2881 caatcagtct cgccttgttt tttattgtat acattagata tggagcaggt taccataaag
     2941 atgattatta gtcgttatta aatagaatcc ttcgtatttt tttgttctat cactacaata
     3001 tctttatttg gacgtttgaa ttgtattccg gcacgttcta gctcctgttt cactcctaca
     3061 tggactttga catcttctcg tgtcattttt aaaacgtgat gaatcgcata gtcgatttct
     3121 tgccttgtga tttttttctt cgtattgatc atcacgactt gctttttatc atatgtgtct
     3181 accaatagca gttgtgttcg atctaacgaa aataatttgt aataattaat agccgtattc
     3241 ccaaaaaaga attccactac tttaggataa aatgtcttaa tatttaaatt actattcaca
     3301 ggtgtttcga ttagcttcat ctcattcctc ttttctatat agtcgattta gatccaatct
     3361 taagttatgt taccatcttt cctagcatta aggaatcggt tacaattgaa cctttaaaga
     3421 caaaaggatt taccttttat cacttaatag tataacataa tttcgtgaga aaaaaataca
     3481 attttttctt gaccatctcg ttacgtaaag gattatgctt acatttgtca ggaggaaaaa
     3541 taatggagta cacaataaag aaaatgtcag agatatcagg agtcagtccg agaaccttgc
     3601 gcttttatga tgagattgga ctgctaaaac cagctaggat caattcttca ggttatcgga
     3661 tttatggcaa aaaagaagtc gatcgtctgc aacacatctt attttatcga acaatggcat
     3721 tcaagttgga tgatatccaa gaggtcttag acaatccatc ttttgaccac cagaaagctt
     3781 taatcaagca tcagcaaatg ttgctggaaa aacgggcaca aatcgatacc ctcttgacaa
     3841 ctgtccaaca gacacttgat atgtacgaag gaggaagaaa aatgagtgac aaagaaaaat
     3901 ttgaagggtt taaacaacaa aagttgaaag aaaatgaaga atcctatgga caagagattc
     3961 gacaaaaata cggaaaaaaa aaggttacag catccaatga aaaatggatg aatatggata
     4021 aagaaacgta tgaagaaatg caaactgttg aaaagcagat gttatttgat ctcactcttt
     4081 acttaaaaga accagaagat gaaagcttag cagaccgtat cttccaagct cataagaagt
     4141 ggttctctta cagctggcct gaatatcagc caaaagcgca taaaggacta ggaatgatgt
     4201 acgaagccga tgaacggttc actgcatatt atgacgagcg aagtggtcat ggtgcagcaa
     4261 aagctttaaa tgaaattatc caaaaaaagg cgtaatcttt tacatgatcc aacaaaaaag
     4321 tacacgtaaa atgacctaat aacaaaacag cagacaccca agttagattt ttatgctaac
     4381 ttttggtgtc tgctccattc tttcacaagt tttttcatcc tactttaaat atattcatcg
     4441 ttgaataaac gtataaggta ttttcacttt atcctttttt tctgtaagaa acaattgaaa
     4501 tgccatttct ccgcaagctt ccagatggtg gtcgatggtt gggaaaccaa gaagctcgct
     4561 gatcagcaaa ttatcgcgtc caatcaccac tggtgtctta tcttgcagat agctttgcaa
     4621 gatgcctgct gcaaccatat ccgaatttgt aaagacacca tcaactccaa gctctttaaa
     4681 aaagaagcct gcttgttttc ctttctctgc atctatacaa tcccaaaaaa caagtgattc
     4741 atcaaaagat ggaaaaagtt gatgacatag ctgcaaagtg atttttgagt tataacttag
     4801 cctgccactt cgccccaacg tgatgcccaa ttgctttata ccttgctgct tcaaataagt
     4861 aagaccttct gtaattgatt gttctcgatc aataaagaca cagcttgcct gttttccctt
     4921 gatttcttca caaaaaacta gaggtccata tttttgatat gccaataaat catctagtgt
     4981 attggcacga gacgtaatga tcaatccatc gaaagcttta gctgcaaatt cttcaagata
     5041 ccttcgctct ttttctttat gatagcccgt cggtaaaagt gtcactttgt aatcataatc
     5101 aaacgctgct tcaatgatcc cactgatcaa ctgatcaaaa tacggatgat tggtataagg
     5161 cagaatcact ccaatgcttt tcgtttttcc ataacttaac tgtctggcat ttgcattagg
     5221 gatataatcg acttctttca tgaccgcaag tatttctttc cttttttcct catctacata
     5281 tggatgtcca ttgattacac gtgaaatagt agaaactgaa taacctgtga gctttgcgat
     5341 atctcgaatg tttgccactg ctgcttcgcc acctatacaa aaagattttc ttgtaaatag
     5401 tctaccacag ctggaccgca ttcaaaaaac tgatacattt ttccttcatg cgaaaaactg
     5461 atccgctgcc cgccccaata tatatctttt gtcaaatttt cgatttcatt cattggtatt
     5521 tcttttacta tattcctatt caacccttga aaactgtaca acgttaatac ggatcccttg
     5581 aactcgacaa acgcataaaa ataattgttc gttgtgccat ctgttatctc tacataagta
     5641 ttcattgcaa gccctccttt tgatactttg agtatagtgt atgaaacgcg tctcatagca
     5701 aggagtttgt tttgccaaag aaaagtttta ttcctctttc attattacca aagtctctta
     5761 ttttaaattg aatacattat aatgatactg taaatatatc tttattatta gtgaat
//
LOCUS       DBNICI010000157         5771 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.160, whole genome shotgun sequence.
ACCESSION   DBNICI010000157 DBNICI010000000
VERSION     DBNICI010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.160"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..302)
                     /locus_tag="AC1ZCC_002410"
     CDS             complement(<1..302)
                     /locus_tag="AC1ZCC_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HIQ1630635.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYV"
     gene            395..744
                     /locus_tag="AC1ZCC_002411"
                     /pseudo
     CDS             395..744
                     /locus_tag="AC1ZCC_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1016..1306)
                     /locus_tag="AC1ZCC_002412"
     CDS             complement(1016..1306)
                     /locus_tag="AC1ZCC_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630636.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1648..1944
                     /locus_tag="AC1ZCC_002413"
     CDS             1648..1944
                     /locus_tag="AC1ZCC_002413"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630637.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1951..3903
                     /locus_tag="AC1ZCC_002414"
     CDS             1951..3903
                     /locus_tag="AC1ZCC_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIQ1630638.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3975..4472)
                     /gene="dfrG"
                     /locus_tag="AC1ZCC_002415"
     CDS             complement(3975..4472)
                     /gene="dfrG"
                     /locus_tag="AC1ZCC_002415"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HIQ1630639.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4799..5491)
                     /locus_tag="AC1ZCC_002416"
     CDS             complement(4799..5491)
                     /locus_tag="AC1ZCC_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIQ1630640.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1895 a          997 c          917 g         1962 t
ORIGIN      
        1 acatacttgc aaagtaatac tacaattcct aacataatcg tagaatttaa taatttccat
       61 agcataaagt gtttagaaaa gaacattaag aatagttcta taataattct tgaactccat
      121 gtcttataat gttctatcga agcacccaaa tagccaagtt catctaatct acttgcataa
      181 actacgtcat ccgctctcat agtcggcata tacatattaa agagtaaaaa gataccccaa
      241 atccctatta gtaacaagat cagttctttg ttattcgtta tttttctatt atgtttgttc
      301 atttttatac tccttcttat atttcctaat agagtataac atagctttaa gcattactta
      361 aagataactt aaaagttatc ttttaaatac ttaattgtta aataccttat ttgatgcaaa
      421 agaaatctac gatagtcaac aaaaaaattt tcattatatc cagaaaaatt tttcttagtt
      481 aaggacctat ggattgcagt aatgcagaat tcgtcgaata aactgccaaa gacatacaat
      541 catattgctt ttaaaattga cgaacaagat atagattcgt ttgtctcaaa aatccaaatg
      601 ttaggtttaa ctgtcgaacc aggacgttct cgtgtgaaag gcgaggcttc ttcaatttac
      661 ttttacgact atgataatca cctcttcgag ttacataccg gtactctcca ggaacgttta
      721 aaatcctaca attccactac ctgagactag taatgactag tctcttttta tggcctctga
      781 tccccccatt gagcgaatac taatagggct tcgaattgat ctgttcaatc tttaagcaat
      841 atccctcaaa tgagtttgga aagtttagtg attcatcaca aatacttaaa tacatgtagt
      901 tatatataga ttgaggttct tgtgagcgta tgctcgtgtg ggttcaaatc ccatcgtctg
      961 catccatctt tccaaaacaa gcggctagga catttttgtc tcagccgctt gttttctagt
     1021 ttattctttc atcttgtcgg accttttttc ttgggtatag acttttctca tattctacca
     1081 caattttggg tttccctaac acatgatagg atctcacaac aaattgcttt ctattattat
     1141 aataacctgt aacgactaac ttcatccctt cgtctacttc agctaggaag ttcaaactat
     1201 gggaagcaat caaacaattg accccattta aactgaaacg gatcaacggt ctggcactaa
     1261 cttgcaaagt acggatctta tctactgttc catatttttt ctccataacc ttcacctcaa
     1321 taccattata cgaacataca ttccctttgt aaagaagaaa ctctgtttta tctttttgac
     1381 tttttgtcta ttacctttct taaaatcata ttggcacttc ctgcgaagat gaaggacatg
     1441 tactaaaaaa aagaggcagc agtatagatt taagtagttc agttttggag tacaaaaagt
     1501 gaaaatgtaa acctaaatga gtatcaaatt gcaactgaat ttgatactcg ttttttattg
     1561 ttaaatcaac gtttgttgtt aatttacacc acaaaatgta agttaaaatg tatataaaaa
     1621 tgcgaacgtg aaataattat tgtaggaatg caaattggtg tgttaaaatg gttacaattt
     1681 actaagcaaa aaggggtgca ttttcctatg caatttcttg agccaaaaaa taagaacgct
     1741 aagtctgtgg attgggaaat atcagagcaa gtaagggtga tagttaagca atacgctgaa
     1801 tatgcagagc gtactgaaag cgaagcagtg gatgaattct tattgaacat tttagacgat
     1861 aagaagttta ttgaatggat tgccaacaag cgaagtaata agcggattgt tgaaaaaatg
     1921 ggtatcaaag atagagtggg ttgatagaca atgaccaagc ttaaaagatt agccacgaag
     1981 gaagatgaaa ttgtagatgt taaaattccg atttcaaacg aagaattaaa agatagggct
     2041 aaacaatatg atttattaac tccaaaacga tttgcaacaa ggtataacaa aatgttgttc
     2101 ttgccaacgt cttttaaatg gaacggttct gaatatccca ttcaatataa ttactgcatc
     2161 aatccgtttt gttgtaattt tgggaaagaa cagcacaaat tcaaagatgt aaaagggaaa
     2221 ccaagtcgat ataaaatgac tggtagttcc aaagataaag gtcataaagg tatgtactgc
     2281 aatgacaatc ctattggcag aggtgtatcg caaaactgta cagtaacccc tttatctaat
     2341 tggtctgttg ttgaagaaat taaacgcctt atagaaatca atagcatcca agatgttgag
     2401 ccagattatc aatttcataa agaaggctgc tcggaagaag aatcaacccc attcaatgag
     2461 ccgaagcaat tttacaaaag ggggaaaagt aggggcaaat ctcaacgcta tcaatgtaag
     2521 gcgtgtaaga agttcactaa tgttctacca aaaagagaag aaaccactac ttatcaccaa
     2581 caaaagaaca ccattcttcc aatgttcgct aagatggttg ttggaagggt ttcagtcagt
     2641 cgtacttgtg atattttagg aattggagtt ggaacctact atcataagct ggagtggctg
     2701 tataggcgtt gtttggagtt tttagaacga tatgagacac aaccccttca aacaaagaaa
     2761 tttaacgaga tgtggcttaa tacggacaag atgcactact acctaaataa cgtccgtaag
     2821 aaaggacaag gttcaaagaa atatacgggg tttgaagacc ttaatatgca aacgtatata
     2881 gttgtatctg ctgaggtctt atcaagatat gtgttccgtt ctgatgttgc ctatgattgg
     2941 aatatttcaa tggatgaatt aaatgaagat acaagaaaat tcaaagaaga ccatctcaat
     3001 acttttagtc gtaaaaatga taggttagat tggtcttatt atccacaaga accatccgct
     3061 aacgatagcg aaaataggaa tgcttatctt cacgaattag ggaaaattac taataggagt
     3121 agatttgtcg atggtttaaa tgttgatgct ccttatacta caactgctca ctattggtta
     3181 attaaacaaa tggttaatgc cgacgaatgg cgaatgatta gtgatgatga cttctctatt
     3241 agaaacgcct tttatagagt attcacgaaa gaattaaggt tgagcgatgc acatcatttt
     3301 atttgccaag ttaacaagac gaaaagcaga aaacaatgct taaaagaatt cggacaagca
     3361 aaagcagaac tgctcgattg gggagatatc agaggattca aaacaaagtt tctacggact
     3421 ttggcatctc attatctaac tgaattacta acaagtcatc aattccacga agaggctatt
     3481 agtaaagatg gagaaagata tcgcaaatat gccgataacc ctatcaaaca tccattagcc
     3541 actaaggata aaggatttta ttcagttgat tgtagaacgg atttatctgc tctggaacca
     3601 aacgaaatcg caaagatgct attaaatgtt aatgaccatt cgaccaatag ctttattcaa
     3661 cagataagac gttatatatc atctcttgaa agaccattaa caacagctcg tggtgataag
     3721 aagagctata tttacgctaa cttcaatccg aagtatgctc aatttgcgat tacaattcta
     3781 agaacttact acaacttttg tcgccctttt aaatcagcgg ataaaaaggt tttgacacct
     3841 gctcaaagat tagggattac agataaacaa tttgattgga aagatataat ttatttcaaa
     3901 tgacaaaaag agcttggagt aatcccaagc tcttgtgctt tatctttact gttcttcttc
     3961 aattaacgca cccgttaact caataagttt tttctttcat atacatgaaa atagtagtta
     4021 tacggatttt tatcattctt tatccctttt tgggcaaata cctcattcca ttcctcataa
     4081 ttcacttctg gaaaaaaagt atctccttcg aattcatgat gtatttttgt gatgtacatt
     4141 ttctcaacat aagggaaaaa caaattataa atctgttctc ctccgaaaat aaaaatttct
     4201 tcttcgtttt tacataactc aaaaacatct tctattgaat gaacaatttc acaaccatta
     4261 aaggtaaacc ccttatctct cgtcagaata atatttcttc tgtcaggtaa ggctcttccg
     4321 attgattcaa ggttcttcct acctaatatt atcggatgtc cctttgtagt atttttaaca
     4381 tattcccagt ccttgggaat cctccaagga atgtcattct ctttgccaat cactctattc
     4441 ttatccatcg cagcaatcaa agaaactttc atttataaca cagccttttt aactaattta
     4501 ccatctaatt tcttatttct atcaaaaaac cttttctcat tatcctgccc gttagttgaa
     4561 taagcaattt tttctataag ttatattatc ataataccac aaaatcacaa attttcattc
     4621 aattatcatt ttcaattgca ttttataaat cattttgtat gtaatttggt atgtaagtta
     4681 aaatgaaaaa agtagtccat tttgaagtac attttgtcat tttgtactcc aaaactgaac
     4741 tacttagtat agattactgt tgcttcttta ctaatttagg tttacttgtt aagaaagatt
     4801 atttacgcaa atgttcttca acaatttgct gagatttatc agctgtatct tccacttcat
     4861 cttttgcttt ttctaacttt tgaatatatt gtttcaagct tgagttttca gcagttgctt
     4921 cgtctaattt cttttgtgta tctgttaact gagtttctac atttgttttt tcttgtgtca
     4981 gtgttgcgat ttgttgattc aaaccattta cagtattttg taattctgaa agttttgctt
     5041 gtgcttcttg tagtcttgct gtccaatctg cattcagatg attgatttca tcattttttt
     5101 ggttgatctc atctagtttg acattgattt cattttgttt gctttgtagt tgtgcatcct
     5161 tgctgactaa ttgactttct aagttttgtt tttctgctgc cgattgattt ttttggtttt
     5221 ctaattcatt tacttttgtt tggtattctt ggacatctgc ctttgaatct tgcaatgtct
     5281 gttctttttg tgctagtcga gcggtaagat ctgtaatcgc ttgctcttta tctgtcaatg
     5341 tatgatcaag ctgtgtcaga ttctcattga ttgtatcaat atctgtctct ccaccccaca
     5401 aaataatttc atttgcgtac cgcgtgcctg tgtagccaat tagacataga aatgcaacga
     5461 caatcccacc agtaataaat ttctttttca tttgcatagc tccttaatcc aatactttta
     5521 catgctaaaa gtaagtgatc tcaaacacaa tgtcaagcgt ttttaaatta catatgtaat
     5581 tttaatataa atactatcaa atactcattt aatcctcctt ttttatctat ttaaaaaatc
     5641 ttttttatag agaaaaatgc aggttaatac tttcttaaat aaatatttat ggtgaacaaa
     5701 aacaaaaaag caagttttat taaacgtaac ccttttcatt tcatagatta ctgtatttat
     5761 tacaaaaaac g
//
LOCUS       DBNICI010000158         5701 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.162, whole genome shotgun sequence.
ACCESSION   DBNICI010000158 DBNICI010000000
VERSION     DBNICI010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.162"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..1233
                     /locus_tag="AC1ZCC_002417"
     CDS             <1..1233
                     /locus_tag="AC1ZCC_002417"
                     /inference="COORDINATES: protein motif:HMM:NF023433.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R,
                     EcoR124 family"
                     /protein_id="HIQ1630641.1"
                     /translation="WQHCVLFFAILCTKLLQNTAHKNPYKTVDEATQLDLVIVVNQLL
                     TGYDSKWVNTLYLDKSLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEA
                     IELYSGKQAKGLFVEKLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVN
                     LYNQLIHCYESAMIQGFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDG
                     NDSIAYDIDYQLPEGSRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQL
                     PVELQPYAEMILNDVNNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALL
                     REMLDLRLTEVNINEYSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRA
                     FLLEDVFDVTEYIKNYFN"
     gene            1966..2934
                     /locus_tag="AC1ZCC_002418"
     CDS             1966..2934
                     /locus_tag="AC1ZCC_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIQ1630642.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            2945..3760
                     /locus_tag="AC1ZCC_002419"
     CDS             2945..3760
                     /locus_tag="AC1ZCC_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HIQ1630643.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKKIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            3828..4538
                     /locus_tag="AC1ZCC_002420"
     CDS             3828..4538
                     /locus_tag="AC1ZCC_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIQ1630644.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            4574..>5701
                     /locus_tag="AC1ZCC_002421"
     CDS             4574..>5701
                     /locus_tag="AC1ZCC_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIQ1630645.1"
                     /translation="MKQVLRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1963 a          849 c         1148 g         1741 t
ORIGIN      
        1 tggcaacatt gtgtactttt ctttgcaatc ttgtgtacaa aactattgca aaacacagcg
       61 cataagaatc cttacaaaac agtagatgaa gcaacccaat tagatcttgt tatagttgtt
      121 aatcagctct taacgggtta cgattcaaaa tgggtcaaca ccctatactt agataagtca
      181 cttgaatatc agaacataat tcaggctttt tcacgcacaa atcgtcttaa tggtcctgaa
      241 aaaccgttcg gaaccattcg ttattatcgt aaagcacata cgatggaaca gaacattgaa
      301 gaggctattg agttatactc aggcaaacaa gcaaaagggt tatttgttga aaaattatat
      361 aataacttag aaaagttgaa cgatatattc gaagaaatta agtccgtatt taatatttct
      421 ggcatagaaa acttcagtca tttaccggag gttccagggg aaattaagca atttgtaaat
      481 ctttataatc aactaatcca ttgttatgaa tcagcgatga tacagggatt cacatgggca
      541 aaatcgactt atgaaatcca ggattatgaa cctataacag tagtaatcac agaatatgaa
      601 tttaatatct tggcacaacg ttacagcgag atagagagag aacctagaga cggaaatgat
      661 agtatagcgt atgacattga ttaccaatta cctgaaggta gtcgtagtcg tattaattat
      721 gagtatttaa acacaaactt caaaaaatac atgaatataa aagaagaacg tgattcgaat
      781 ttaattcaat cattgttaaa cgacttacat cgcacatttg cgcaattacc agtggagtta
      841 cagccatatg ctgagatgat tctaaatgat gtaaataatg gggaattagt tatcaaagaa
      901 ggatgggaat ttactgatta cctcaatgaa tatcagctta gtgaagagga cgattttatc
      961 caagatttag tggattcaac caatattaat gaggcattat tacgtgagat gttggattta
     1021 cgcttaaccg aggtaaacat taatgaatat agtcgatttg ataaattaaa atcaagtgtt
     1081 aacgtaggtc atttcagtgg atatatcgag aaaaatttag gtaaaatggt aataccaatc
     1141 aaggtaaata tgctaattga cagattattg cgtgcatttt tattagaaga tgtattcgat
     1201 gttacagagt acataaagaa ttactttaac tagagtacac ctacatctta tagtacataa
     1261 aaaaattcat cttttttagt ttaataattc accaaaaagg tgagtaatgc catttttgaa
     1321 aattgagctt gttttatgtc tacatctgta tccgtatata atccaaagga ttataaactc
     1381 agaagagtct ttattttttc tgaatataac tactgtatca aaaacaatcg attatgagac
     1441 aaaacaaaat gacctctttt atttaagaaa ggaggtcatt ttttgtatca taaattgtat
     1501 taaaatcaat gtatcacttt ttaatcaatt ttaagaccat aaattgcgta ctaaaagaaa
     1561 ttctgcaatt caaaataaag cattttaagc cattttcaaa tttaagtgca cccatctacc
     1621 cgtcaaatca ctttattttc cactccagta gtttcctaat tcaactgtgt gcaaaacgat
     1681 ttcttctaaa caaatcaaga caaacttata aaccttcatc ccagcaccat aattatttaa
     1741 atcataggca tttttcgaaa ttagtatatg tactcaagtt tcactgcaga ttatttatat
     1801 tttttagaga aataaatttt tcctattgac aaacacaaag aaagcggata caatacaatt
     1861 atgtcacatg acgttatatg acgttatgta atatctttac atttaatcag ttacttttat
     1921 tttggataaa attaacattt ttaaaatttt aaggaggaaa tgttgatgga tgtagcaaaa
     1981 acggttaacg caattgtaga aaaaaatgat ggagagattt ctggaattta tttggtggct
     2041 tgtggcggtt cgctggtaga tatgtatgtc tcagattatt ttcttaaagc cgaagcatcg
     2101 aagtgtatta caggcttata tacagcgaac gaatttaacc atgtaacccc aaagcaatta
     2161 gatgataaat ctgttgtcat tctatgttct catggaggaa atacagcaga aacggtaaaa
     2221 gcaggagaag tcgctcaagc tagaggagct gtgactgttg gtctgactca taataaaact
     2281 gcggatttat taaaggtttc tgatcatagt ttcctttacg aatggggaga tgatgcaaaa
     2341 atagtcaata atcctatggc catcattctt gatttaactg tatctttggt caatgttacc
     2401 gaaacatttg aacattacgg taaattcaga gaaggcatga aggttatcga taccgtaatt
     2461 gaaaatggca ttaagcaggt gcaggatcgt attaaagtgt ttgctgataa gtacaaagat
     2521 gaaacaatgt tttatatctt aggtagtgga ccatcatatg gacatgctta tggtttttca
     2581 atttgctctt tgatggaaat gcaatggctt gattcaactt ctgttcattc tggagaattc
     2641 ttccatggac cttttgaagt aacagacaaa gatacgaatt ttattctctt gatgagcatt
     2701 ggacgcactc gtgctttaga tgaacgggca aaacgattcc ttgataaata tgcagaaaga
     2761 gtagaaatcg ttgatgcaaa ggaactagga ttggatatga tatcagatga agtttctgaa
     2821 ttcttcaatc ccattttatt ctacagtatt ctttcagaat atcgttcagc tttagcagat
     2881 gcacgcaatc atgatttaga tgttcgtcgc tatatgggga aagtggagta ctaggaggat
     2941 taaaatgaag gtattaggct taggtgataa cgtcgtcgat aaatatgaaa atctgggaat
     3001 tatgtatcct ggaggaaatt cattgaattt tgcagttttt gcaaaaaaac tgggacatga
     3061 aagtagcttt atgggagttt ttggctcaga tgaggaggca gctcatgttt tatcagcaat
     3121 aaaaaaaata ggactggaca acagtcactc tcgctttgaa aaaggcgaga atggctgtgc
     3181 cagagtgaag attgatgagg gcgaccggat tttccttggc agcaattcgg gaggtgttac
     3241 tcgtgaatac ccgattcagc ttactgaaga agacagagaa tatttaaacc agtttgaatt
     3301 gattcatatg ggattatata gccatgtcaa tcatctatta gaagagttac aaaatgtttc
     3361 tggaaagatt tcttatgatt tttctgacga ctttacggaa gaagaggttc agcgggcaat
     3421 tgataaagtt gattttggct tcttttctat agaagacttt tccgatgaag aagtaaagca
     3481 gtttttgatg aagcattttt gtgagcgaaa tgatgtattg attgcaaccc gaggagaaaa
     3541 aacggctatt ggttatgatg gagcaaagtt ttatgaagtg aaccccgtgc tagaagaacc
     3601 agttgataca atggcagcgg gagattcttt cttaacagct ttcctacttt attttctcga
     3661 aggggacgat atagtcaccg cgatgaaaaa ggggaatgaa tttgctggga agtcttgtct
     3721 ggttgacggt tcgttcggct ttggtattca ttacgattga gcttgtttta aattcctgca
     3781 tttgataacc taaagaaaga aatcccggta aagtggaggt taaaaaaatg ctggatacaa
     3841 atgcacaatt accattgtac ttacaattaa aagaaatatt aaaaaataaa atcaatacag
     3901 gggagtatcc cgagaatacg aaaattcccg ctgaatcaga gctgatagaa agttatgagg
     3961 tcagccgaat cacagtgaga aaagcagtag aagaactggt agaagaaggg tacctactta
     4021 aaaaacaagg gaaaggaact tttgtcaacc ggcataaagt ttttcgtaaa attgaatatg
     4081 ttatgggttt cagtgagagt tgtaaatcta atggttttac accgactagc attgttttgg
     4141 aacgcaagat cattccagca actgaaggga tggcaaaaaa actcgatatc gctcaaggtg
     4201 atgatgtttt atatatccag agaaagcgaa tggcggataa catccccatt atgttagaaa
     4261 acaattattt ttcttatgaa agatatagtt tcttaatgga ggaagagctg actgattctt
     4321 tgtatgaagt acttaaagtc caaggtacct tagccatcaa tcctggggaa actgcacttg
     4381 aaattgtcac agctgatgac caaattgcca aaataatgga agtagctgtt gggacgccct
     4441 ttttctttat ggatacttta atcaacgatc aaaacaacat accaattcat gtaggtaatc
     4501 aatattacct gggagaagta tatcgttttt acttatgatt tcctgttttg atttcaagga
     4561 gtagatttta tttatgaagc aagtattgcg gtcaaaaatg atttttgatg gagagcagcc
     4621 acctttcaaa ggttatattg taatcaatga agggatcatt gaaaaggtgg aaaacaactg
     4681 gaattacagt gatctattaa atgaaaacac ggaattaatt gcttatgatg atcaattcgt
     4741 tatgccagca attcatgata atcatgtatt tttcagtggt tatttggcta tgaatgctgg
     4801 catagattta agcgatacat cttcagctaa cgaggctgta gagcgtatta aagctgtaat
     4861 gaaccataaa aaagcagatg aaccaattta tgctcatggc tgggatgctg ccctgtggaa
     4921 aactgaccca tcagaagagc tattgaatga aataaaccac tttggtccaa tcacagcgat
     4981 cgacaaaaat cgcagccatt gttggatgaa tgctattgct aagcagactt atggttttac
     5041 agaaaaagaa accagtgctg aaagtcgagt attattaatt aaggaaatgc tagcaaacaa
     5101 aaagctttta gcagaggttt acgctgaatt tgaagcatta ctgctttcaa aaggtgttat
     5161 atcaatgaaa gaaatcgttt ttgatgacgc agaatttctt tctaatatac ctcagaggaa
     5221 aatacttagt aacctatatg tgcaggcagt ccaagaacct ctacagcatg aacttctata
     5281 tgagtataag gaaaaagttt tccctgaaaa ggtccgcttt ggtggagtga agatcatggt
     5341 agatggtgtt gttgcggata gaagtggaga tatttacggt gtttatcagt caggagtagc
     5401 ttcacccgag attgactatg aggcaattaa agcagaagtt gctgcattca atgaagtaga
     5461 gattccttgc tgtttgacaa cggaaggaga taaagcgggt tataaagctg cgtcaattct
     5521 tgtcgaattt ggaaagcggt tacctgaagg cgtttttaat tcgattagtg atttggaaat
     5581 ggttactcaa aagacagcag aaaaaatgaa cgaaggacaa gtagtagcag aggtatatcc
     5641 acaaattctc ggcttgaatc aaacgttaga agaggcttac atgtcaactg ttataaaaaa
     5701 t
//
LOCUS       DBNICI010000159         5683 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.163, whole genome shotgun sequence.
ACCESSION   DBNICI010000159 DBNICI010000000
VERSION     DBNICI010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.163"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..192)
                     /locus_tag="AC1ZCC_002422"
     CDS             complement(<1..192)
                     /locus_tag="AC1ZCC_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630646.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFE"
     gene            complement(726..2885)
                     /locus_tag="AC1ZCC_002423"
     CDS             complement(726..2885)
                     /locus_tag="AC1ZCC_002423"
                     /inference="COORDINATES: protein motif:HMM:NF033527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HIQ1630647.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSGK"
     gene            3031..3606
                     /locus_tag="AC1ZCC_002424"
     CDS             3031..3606
                     /locus_tag="AC1ZCC_002424"
                     /inference="COORDINATES: protein motif:HMM:NF012464.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIQ1630648.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3820..4515
                     /gene="vanR-A"
                     /locus_tag="AC1ZCC_002425"
     CDS             3820..4515
                     /gene="vanR-A"
                     /locus_tag="AC1ZCC_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HIQ1630649.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4493..5647
                     /locus_tag="AC1ZCC_002426"
     CDS             4493..5647
                     /locus_tag="AC1ZCC_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HIQ1630650.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1774 a         1096 c         1077 g         1736 t
ORIGIN      
        1 ttcaaaatct aaaatcaaca catcatgggg agtgtgataa taaatatttt cccttaatgt
       61 accaacaatg gtaattcggt caattgacca ttgcattttt aagggggtgc acatagtttg
      121 tgcatttaac cgcctgttag aaatacggtt aagtttttcc cactcattta cgattatgcc
      181 ttttttactc aaaaatagct cctcctcaac gaaaaaagag attcttggaa agaatttcca
      241 gagttcacgt aactctaaaa atccacatcc aaaaatctct agcttgaaga aacctaaaaa
      301 tattcgtata atgaacttga gttacggaac taggtgaagt agtttccgta aaggtcgaga
      361 gtgcgtcaac actgctcgac tattttttta ctcttttttc tcattttctt tgattatagc
      421 actcctctag aaaaaataaa aagacctcag ggaatttcat tccccgaacc ccttgagcct
      481 cctcctgccg aaatagaatg gcgctgccag catttatttc gttcagctat cggctacaca
      541 atctaacacg accgttccgc taccagtcgc cttacaggct ccattttcgc tcctctaacg
      601 tgtttcaccg attgtgccga taactaaacg aaataaacgc taaaaccgct cagaacgcaa
      661 aatatgccat tttagagcta ttgtagaaaa tatagtttga aatgcaattt tcttctttca
      721 gctttttatt tcccacttgt aaatcggtag atggtggctc cttttccagt tccataatgt
      781 ggatttgcat ctgcatgtag tgatgaaaca cctagctgca ttctcatacc atctgacgaa
      841 gatgttgtac cgtcgcccca atagaaaggc aattgtaatt tatgatgaaa gtttactaat
      901 atggcttggg ctttattcat ggcatcttca tacatgcgcc attgagatac attggctagt
      961 tgcttatatg taagtccggg tgtggcttcg gccatcttgc tcaagccaat attcattccc
     1021 attcctaaaa gggcagccat gataatgatt gtttcttcct tatctggttt tcgattattg
     1081 gaagcatgag tgaattgctc atgaaatcct gttatatggg ccacatccat gagtaaatca
     1141 gttaatttta ttcttggtag catctgataa aggcttgcac taaatttttt tgcttcttct
     1201 ggaacatctt tttctaagcg tgcaagtgat agctttcctt tttcaagaga aaccccatct
     1261 aacttattgg aattggcagc taaccacttt aacctttcat taaagctgct ggttctctcc
     1321 gttatataat cttcgaatga taaactaact gataatctcg tattcccctt cgattgattc
     1381 catgtatctt ccgaaaacaa atattcctca aaatccctat attgtctgct gccaacaatg
     1441 gaaacatctc ctgcccgaac atgctcccga agttctgtta aaacagccat ttcatagtaa
     1501 tgacgattaa ttgttgtacc atcatcctcg tataaatgtc ttttccatcg ttttgaaata
     1561 aaatccacag gtgagtcatc aggcactttt cgctttccag attcgttcat tcctcggata
     1621 atctcaacag cttgtaaaag tggctcattt gcctttgtag aatgaaattc caatactctt
     1681 aatagcgttg gcgtatattt tcttagtgaa taaaaccgtt tttgcagtaa gtctaaataa
     1741 tcatagtcgg caggacgtgc aagttcctga gcctcttcta ctgaagagac aaaggtattc
     1801 cattcaataa ccgattctaa aaccttaaaa acgtctaatt tttcctctct tgctttaatt
     1861 aatgcttgtc cgatgttcgt aaagtgtata actttctcat ttagcttttt accgttttgt
     1921 ttctggattt cctcttgagc cttacgacct tttgataaca aactaagtat ttgcctatca
     1981 tgaatttcaa acgctttatc cgttagctcc tgagtaagtt gtaataaata gatggttaat
     2041 atcgaataac gtttattttc ttgaaagtca cggaatgcat acggctcgta tcttgagcct
     2101 aagcgagaca gctgcaacag gcggttacgg tgcaaatgac taatttgcac tgtttctaaa
     2161 tccattcctc gtatgtattc gagtcgttct attattttta gaaaagtttc gggtgaagga
     2221 tgacccggtg gctcttttaa ccaacccaat atcgttttat tggattcgga tggatgctgc
     2281 gaggtaataa tcccttcaag cttttctttt tgctcatttg ttagagattt actaaccgta
     2341 ttaaatagct tcttttcagc cattgccctt gcttcccaca ccattctttc aagtgtagtg
     2401 atagcaggca gtataatttt gttttttctt agaaaatcta tgcattcatg cagtagatga
     2461 atggcatcac cattttccaa agctaattga tgaaggtact taaatgtcat tcgatattca
     2521 ctcagggtaa aagttacaaa gtcgtattca cttcgaattt ctttcaaatg atcccaaagt
     2581 gtattttccc tttgaggata atgatcaagc gaggatggac taacaccaat ctgtttcgat
     2641 atatattgta tgaccgaatc tgggatgctt ttgatatgag tgtatggcca accgggatac
     2701 cgaagaacag ctaattgaac agcaaatcct aaacggtttt cttccctcct tcgcttatta
     2761 actatttcta aatcccgttt ggaaaaagtg aagtaggtcc ccagtatcca ttcatcttca
     2821 gggatttgca taaaagcctg tctctgttcc ggtgtaagca attctctacc tctcgcaatt
     2881 ttcattcagt atcatcccat ttctgtattt tcaatttatt agttcaatta tatatcaata
     2941 gagtgtactc tattgataca aatgtagtag actgataaaa tcatagttaa gagcgtctca
     3001 taagacttgt ctcaaaaatg aggtgatatt ttgcggaaaa tcggttatat tcgtgtcagt
     3061 tcgactaacc agaatccttc aagacaattt cagcagttga acgagatcgg aatggatatt
     3121 atatatgaag agaaagtttc aggagcaaca aaggatcgcg agcaacttca aaaagtgtta
     3181 gacgatttac aggaagatga catcatttat gttacagact taactcgaat cactcgtagt
     3241 acacaagatc tatttgaatt aatcgataac atacgagata aaaaggcaag tttaaaatca
     3301 ctaaaagata catggcttga tttatcagaa gataatccat acagccaatt cttaattact
     3361 gtaatggctg gtgttaacca attagagcga gatcttattc ggatgagaca acgtgaaggg
     3421 attgaattgg ctaagaaaga aggaaagttt aaaggtcgat taaagaagta tcataaaaat
     3481 cacgcaggaa tgaattatgc ggtaaagcta tataaagaag gaaatatgac tgtaaatcaa
     3541 atttgtgaaa ttactaatgt atctagggct tcattataca ggaaattatc agaagtgaat
     3601 aattagccat tctgtattcc gctaatgggc aatattttta aagaagaaaa ggaaactata
     3661 aaatattaac agcctcctag cgatgccgaa aagccctttg ataaaaaaag aatcatcatc
     3721 ttaagaaatt cttagtcatt tattatgtaa atgcttataa attcggccct ataatctgat
     3781 aaattattaa gggcaaactt atgtgaaagg gtgataacta tgagcgataa aatacttatt
     3841 gtggatgatg aacatgaaat tgccgatttg gttgaattat acttaaaaaa cgagaattat
     3901 acggttttca aatactatac cgccaaagaa gcattggaat gtatagacaa gtctgagatt
     3961 gaccttgcca tattggacat catgcttccc ggcacaagcg gccttactat ctgtcaaaaa
     4021 ataagggaca agcacaccta tccgattatc atgctgaccg ggaaagatac agaggtagat
     4081 aaaattacag ggttaacaat cggcgcggat gattatataa cgaagccctt tcgcccactg
     4141 gagttaattg ctcgggtaaa ggcccagttg cgccgataca aaaaattcag tggagtaaag
     4201 gagcagaacg aaaatgttat cgtccactcc ggccttgtca ttaatgttaa cacccatgag
     4261 tgttatctga acgagaagca gttatccctt actcccaccg agttttcaat actgcgaatc
     4321 ctctgtgaaa acaaggggaa tgtggttagc tccgagctgc tatttcatga gatatggggc
     4381 gacgaatatt tcagcaagag caacaacacc atcaccgtgc atatccggca tttgcgcgaa
     4441 aaaatgaacg acaccattga taatccgaaa tatataaaaa cggtatgggg ggttggttat
     4501 aaaattgaaa aataaaaaaa acgactattc caaactagaa cgaaaacttt acatgtatat
     4561 cgttgcaatt gttgtggtag caattgtatt cgtgttgtat attcgttcaa tgatccgagg
     4621 gaaacttggg gattggatct taagtatttt ggaaaacaaa tatgacttaa atcacctgga
     4681 cgcgatgaaa ttatatcaat attccatacg gaacaatata gatatcttta tttatgtggc
     4741 gattgtcatt agtattctta ttctatgtcg cgtcatgctt tcaaaattcg caaaatactt
     4801 tgacgagata aataccggca ttgatgtact tattcagaac gaagataaac aaattgagct
     4861 ttctgcggaa atggatgtta tggaacaaaa gctcaacaca ttaaaacgga ctctggaaaa
     4921 gcgagagcag gatgcaaagc tggccgaaca aagaaaaaat gacgttgtta tgtacttggc
     4981 gcacgatatt aaaacgcccc ttacatccat tatcggttat ttgagcctgc ttgacgaggc
     5041 tccagacatg ccggtagatc aaaaggcaaa gtatgtgcat atcacgttgg acaaagcgta
     5101 tcgactcgaa cagctaatcg acgagttttt tgagattaca cggtataacc tacaaacgat
     5161 aacgctaaca aaaacgcaca tagacctata ctatatgctg gtgcagatga ccgatgaatt
     5221 ttatcctcag ctttccgcac atggaaaaca ggcggttatt cacgcccccg aggatctgac
     5281 cgtgtccggc gaccctgata aactcgcgag agtctttaac aacattttga aaaacgccgc
     5341 tgcatacagt gaggataaca gcatcattga cattaccgcg ggcctctccg gggatgtggt
     5401 gtcaatcgaa ttcaagaaca ctggaagcat cccaaaagat aagctagctg ccatatttga
     5461 aaagttctat aggctggaca atgctcgttc ttccgatacg ggtggcgcgg gacttggatt
     5521 ggcgattgca aaagaaatta ttgttcagca tggagggcag atttacgcgg aaagcaatga
     5581 taactatacg acgtttaggg tagagcttcc agcgatgcca gacttggttg ataaaaggag
     5641 gtcctaagag atgtatataa ttttggctct ttgtcaaatg atg
//
LOCUS       DBNICI010000160         5614 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.164, whole genome shotgun sequence.
ACCESSION   DBNICI010000160 DBNICI010000000
VERSION     DBNICI010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.164"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(60..611)
                     /gene="hpf"
                     /locus_tag="AC1ZCC_002427"
     CDS             complement(60..611)
                     /gene="hpf"
                     /locus_tag="AC1ZCC_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIQ1630651.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(739..1458)
                     /locus_tag="AC1ZCC_002428"
     CDS             complement(739..1458)
                     /locus_tag="AC1ZCC_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIQ1630652.1"
                     /translation="MRCSCCGKDIIQNLTFSEIIGVSKSRLPQTCSVCRAQFHLLEAK
                     GCAGCQKPIHLKYEETKKKANTSFVYCNDCQEWKKKYPAYDFQHRAFFTYDEKMQAWI
                     NRYKFMGDIRLAGTFAYKWTQIKKDYPAYIYCPIPLSEKRWLERGFNQVSEMLKAAKI
                     PFQMLLKREQHLLAQAKNTRKERLEMPQPFELAVPLSKIQHKKILIIDDVYTTGQTLF
                     HAADCLQNAQPKIIRTISLAR"
     gene            complement(1455..2768)
                     /locus_tag="AC1ZCC_002429"
                     /pseudo
     CDS             complement(1455..2768)
                     /locus_tag="AC1ZCC_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            complement(2849..3169)
                     /locus_tag="AC1ZCC_002430"
     CDS             complement(2849..3169)
                     /locus_tag="AC1ZCC_002430"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIQ1630653.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
     gene            complement(3277..3564)
                     /locus_tag="AC1ZCC_002431"
     CDS             complement(3277..3564)
                     /locus_tag="AC1ZCC_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7662 domain-containing protein"
                     /protein_id="HIQ1630654.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            3691..4335
                     /locus_tag="AC1ZCC_002432"
     CDS             3691..4335
                     /locus_tag="AC1ZCC_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HIQ1630655.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(4394..5101)
                     /locus_tag="AC1ZCC_002433"
     CDS             complement(4394..5101)
                     /locus_tag="AC1ZCC_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIQ1630656.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5321..5491)
                     /locus_tag="AC1ZCC_002434"
     CDS             complement(5321..5491)
                     /locus_tag="AC1ZCC_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630657.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         1615 a         1176 c          977 g         1846 t
ORIGIN      
        1 aacttgcggc agctgtcagt agctgctgca agttttcgtt gttatttaag taaggctgat
       61 cagtctgttt caatcagtcc gtattttcca tctttacggc ggtaaacgat acttgtacca
      121 tttgtttctg catcttcaaa aataaagaag ttatgtccca gcatgttcat ttgtaagact
      181 gcttcttcac tatccattgg tttcaatgaa aggcgtttcg tacgtacgat atctaattca
      241 gaatcatttt cgttttcgtc ctcttcattg aataaagcag cagcatctgc agtattcatg
      301 cccgtttcac gggatttacg attaattttt gttttgaatt tgcggatttg gcgttccaat
      361 ttgtcaacaa ccaaatcgat acttgcgtat aaatctggag aagtttcttc ggcacgtaat
      421 acaagataag gtaatgggat cgttacttcc acttttgctg ttttttctgt ataaactttt
      481 aagttcacat gcgccgttgc atcgggtgcg tcactgaaat agcgttctag tttgcctact
      541 tttttctcaa cgtagtcgcg aatcgcttca gttacttcga tattttcgcc gcgtacatta
      601 tatctaaaca taaaaattac ccctttcgtc taccacaatc aagaaggaga aggaccactc
      661 tttccctctt cttaattcta ttatatcgaa ctctaggtta tttgcaaatt aaatcgctat
      721 caaatttcca catctgattc atcttgccaa agaaatggtt cggatgattt tcggctgtgc
      781 gttctgcaag caatctgctg catgaaaaag cgtctgtcct gttgtataga catcgtcaat
      841 aatcaatatt tttttatgtt gtatcttaga aagaggtacg gcaagctcaa aaggctgagg
      901 catctccaat ctttcctttc gcgtgttttt tgcttgagca agcagatgtt gctctctttt
      961 gagcagcatc tgaaaaggaa tttttgctgc tttcaacatc tcgcttactt gattgaatcc
     1021 tctttctagc cacctttttt cagataaagg tattggacaa taaatatatg ccgggtaatc
     1081 tttttttatc tgagtccatt tatatgcaaa agtccctgct aatcggatat ctcccataaa
     1141 cttgtaccga ttgatccaag cctgcatctt ttcatcgtac gtaaaaaagg cgcggtgttg
     1201 aaaatcatat gcaggatatt tcttcttcca ttcttgacag tcgttgcagt aaacgaaact
     1261 agtatttgct ttctttttcg tctcctcgta tttcaaatgg attggttttt gacaacctgc
     1321 acaacctttt gcttctaata aatggaattg tgcgcggcaa actgaacatg tctgaggaag
     1381 tctagatttc gacactccga tgatctcgct gaatgttaaa ttttgaatga tgtctttacc
     1441 gcagcaacta catctcacag catctgcttc ccttcttcgt tcatttcttt gatttcctga
     1501 caggcttttc gaatagctga agttttttct tcatagaaaa aatagactcg actgttcgta
     1561 taaggacctt tacgatcagc tcttcctgcg atttgcacca aagaggattt agtaaaaact
     1621 ggatgatctg ctcctaaaac gatgacagag attctttcaa atgtgactcc tctttctagg
     1681 atcattgtgg ttaacagtat ctcgtatatt gcttctcgca ttttctgaac tttctttgcc
     1741 cgttgatcgt cttgtgaaga aacatctgtt atctcatatt ctggaaaagc ttcttgcagt
     1801 gtgattttta aacgatgcat catcgtaata cttggacaaa ataaaaggat acggttttct
     1861 ttgattagtt gctgaatgat ccgcaccaaa gagttgattt tctttttgtt tccacatttt
     1921 tgtcgccaat tattcatgaa aacaagcacg ggttcaggaa gcaagcgccg atgaaagcgc
     1981 aaggcaagtt tgtggagctc aaatgtctgc ttgatttcct ttagtaactt ctcatcaggc
     2041 gtggcactta agtagatgaa tcggccgttt gatctgattg ctgtttgcac agcgtgagct
     2101 agcccagcat ccccgttata aggaaacgca tcgatctcat cgatcactat taaatcaaag
     2161 gaccgataaa aacggtatag ctgatgaatc gtacagataa caaaagaagt aaaggtataa
     2221 gccgttgctg tattcccatg gagcaacgtg attttttctt ctggaaaaac ataacgatag
     2281 cgtaaaaaca attcattaca aacatcgact cgcggtgtac aaatcgctac tcttcctcca
     2341 gcttttaaag tttccactaa aataggataa agcatctctg ttttccctgc acctgttaca
     2401 gcccaaataa gatgatgtct tttttctgaa tggcaaagta ttctctttgc gatttccgtt
     2461 tgtttttcag taagctgtcc tgtccaaaaa aagacgactt ctcgaggttt tgcttcaggc
     2521 tgatcaaaca aatataaatc ttgttgagtg tcgcatcacc ctaaatatag acaatgtgta
     2581 caaaaataac cgtaatctgc aagctgtcca acaatgacgg acgtcctcat tccacagcga
     2641 tgacaaagaa tccagttgcc caaacgttcc actgctgcga tcttctcata atttggtaaa
     2701 tttacctcct cacagtctat tgctttaata tctctcgtca gaagccttct accccaaaaa
     2761 tgattcatat tattcgccct aaaaaaatat acgaaaaaac aagcgatcta ttttttggat
     2821 cgcttgttcg atagttatac tgttctattt attcacctat gagcgcgatt gcttgatcta
     2881 acactttttc gccattcatc ataccataat cagccatatt gatgacatct aaaggaatgc
     2941 cttttggttc cagacgtttc tcaaaatcag ctttcataaa gcgaacttgt gggcctaata
     3001 acaacacatc cacgtcttta tttgcaagat tgttatctgc gtcagaagcg gacacagcga
     3061 aaatatcaga gtcgattcct tttgcttcag cagctttttg catttttgtt actagtaaac
     3121 ttgtgctcat accagccgaa catacaagca tgattgtttt tttagccata tttttctctc
     3181 ctttttaaaa caaactacgc aaacgttttc ttcgcgtctg ttactttatt atataaaaag
     3241 aaagaaattt gtcaacgttt tctaaacgcg gtttatttaa tcaaatagcc aactagattt
     3301 cactaattta ttgaacacga catattcttt gtccaaattg atgacaacta cttcatagcc
     3361 tgcatttatc cagccatagg cacctttttc agggtgttta taatcatttg cccaccaatc
     3421 tgtactttcc cttgctgttt tgggaagtcc gttactggga aacagcagtt cgtcaaattg
     3481 ggtaaaggtc aacgttactt gagagccgcc attgttcttc aaataatgcg taattgcatc
     3541 gtactttttc attcgttcct gcatccccag atccacctgc ctttgtcttt attatcgcat
     3601 actacagcaa ttgcgcaacc gttacagtgt tgaaacaacc tagaacttta tttatactga
     3661 ataaagaaaa tgaaaaaaag aggtgacaag atgatcgagc gctatcgtac aataaaagaa
     3721 aatggacaaa gtgagattga gatcaaaaaa tcacgcttta tctgttctct tagacgaata
     3781 gaatcagagg accaagcaaa aaatttcatt caagaaataa aaaaagaaca ttggaaagcg
     3841 aaccataact gcagcgcctt catcattgga gaaaaaaatg aaatccagcg aagcagcgat
     3901 gatggagaac caagtggaac ggcgggcgta ccgatgttgg aagtcctcaa aaaaaatcaa
     3961 ctgatcaatg tagctgcagt agtcactcga tattttggag gaacgaaact cggcacaggc
     4021 ggattaatac gagcatacac tcatgcggtc tctcaagcat tgtctgacat tggaatcgta
     4081 gaaggtacct tgcaacagga gatctctgtg atgatcacgt atccgcaact aggaaaatta
     4141 cagaattatc ttgaactcca acattttttt ataaaagaaa tccaatatac tgatcaagtt
     4201 agcgtcatct gtgctgtaga tgaaaaaagt gctaaccact ttaaagaatc catcattgag
     4261 ctgctgaacg gacaagtcca atttgaagag ggttctgttt cttatcacga gcaactgata
     4321 actgatcata tataaaaaca ggtgattctg attctctttt atttctaata aggaatcagg
     4381 gatcacctgt tttttatttt tccaaataaa actcaaaacg atttcctacg tattgcgtac
     4441 gcacatactc aaacggcgta ccgtcttcaa aataagaaat ctggcgcaaa cgaagaactg
     4501 catctcttcg ttttacctct aaatagtcag caattttctc agaagccaac acagcagtaa
     4561 tcgtttgatt cgctggaccg atctttttcc cgcctttttc ttctagcgtg cgatacaacg
     4621 attcagtaat ctctgctttg ctgtagccat cgatcagctt ttgtggaaca ctggcaactt
     4681 caaaacaaat agggacatca tctgcgaaac gaatacgttc cattcgcaag atcgtgtcat
     4741 ctttactcaa gtttagtttt tccatttcac ttgagcttgg cgaagtcaag aaataagaga
     4801 ttgtccgact agacggtact cgattttgtg attccataat ttctgtaaaa cttgttgcac
     4861 cagacatttt ttcttgtact ttttctcgcg caacatacgt acccgaaccg atctttcttt
     4921 ctaagattcc ttcatctgcc aagttttgaa ttgcttgtct caacgtcata cggctgacat
     4981 tgaatttcac tgctaattct ctttctgaag gaagacggtc tccgactttc catatacctt
     5041 gttcgatttc attttttaat tggtcatgaa tctgtatata tacaggtaat tgattcgaca
     5101 taacgtacac ccgtcctttc gtttcttcta ttataactgg tatatacctg attcacaact
     5161 gaaaaagata aaggtttcta tccatcaaca gaggatttag acacacatcg ttctccctac
     5221 tcctattgtc agaaacaatt ttttagatat gttcaaccta gaaaataaca aaagacccta
     5281 aaatcgatga aaatgccgac ttatggatct tttgttatta ttagcggttt ttagccatcc
     5341 atttttttgt atgattcgtt gggcgttttg attttacctg gaccgctttt ccgccttttt
     5401 tcggaatatt cacttcaata ttgtattttt ctgatttgac caaatcatat aacttttcag
     5461 ctgcttcgtc tactttttct ttccatttca tgtggatccc tccaattctt gtagttaact
     5521 taactctatc ttaattcagc agaatcttca aggaacaagg tagcaaagca aaaaaaaaga
     5581 gctctcgaac taacgagagc ttacgattgt ggta
//
LOCUS       DBNICI010000161         5493 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.165, whole genome shotgun sequence.
ACCESSION   DBNICI010000161 DBNICI010000000
VERSION     DBNICI010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.165"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(57..1010)
                     /gene="menA"
                     /locus_tag="AC1ZCC_002435"
     CDS             complement(57..1010)
                     /gene="menA"
                     /locus_tag="AC1ZCC_002435"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HIQ1630658.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1023..2126)
                     /locus_tag="AC1ZCC_002436"
     CDS             complement(1023..2126)
                     /locus_tag="AC1ZCC_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIQ1630659.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2216..3151)
                     /gene="pplA"
                     /locus_tag="AC1ZCC_002437"
     CDS             complement(2216..3151)
                     /gene="pplA"
                     /locus_tag="AC1ZCC_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HIQ1630660.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3453..5387
                     /locus_tag="AC1ZCC_002438"
     CDS             3453..5387
                     /locus_tag="AC1ZCC_002438"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIQ1630661.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGKSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
BASE COUNT         1593 a         1073 c         1099 g         1728 t
ORIGIN      
        1 tttggctgtt cttattactt gatgcagggc agttttttca caacctcttc atgctgttaa
       61 atggcttgca taatgattgt tagtaatagc cccaatacat aactggcatt gaataagacc
      121 atatttttga tggaatagct gaaactgata ggctggggta acgtttgctt gaattgatcc
      181 aaatgcttct tcacaattgg caaggtcaag aaggtgacca agatcggcca ttcatagata
      241 ccgctcaaca accccattaa aatcacgaaa tagcaagcat acatcaatag ctggaataag
      301 actgttcccg caggacgtcc gatataaaaa actagtgtat agcgatgatt ttcaatatca
      361 cggtcaagat cccgcaaatt attagccaac atgatattgg caatcgtaaa caccaagggt
      421 aaggaagcta atacgattgc tgctatagcc caaagatttc ctaccaaagc aaattgtcca
      481 gatgaaaagt tgactgtcag atcatagaca tctgaaccga atgtattgac ataaacgacc
      541 atggcaaaaa ttccaaggcc catagtcacc ccactgaaaa tttcccctaa aggcattcga
      601 gataaaggaa tcggtccgaa tgtatagaat actccaataa aacaacagat aaatcccatc
      661 aaaagtaaaa gccaacccgt attgatcgtt agaataacgc cgataacaaa aacaaatgca
      721 atcatagcta aaatcatgtt tcgaatcaat acaggagata gatttctgcg gccaatgaca
      781 ttttcttcat acttatatac ttcagaatga gccttttcat aatccatgta attattgata
      841 gcggtagtcg ctaaatcaaa aactagcatt ccaataaaaa ataaaaaagt gtttagccaa
      901 tgtatctcat gaaaaaaagc gatagagaat aataccccta ccgcaaaagg aaatacacta
      961 gccacctttg ttcgtatttc aactacttct aaaaagattt ttagtgtcat ggttcgtact
     1021 ccttatttta gttcagaacg gtcacccatc gtgtaatggg agtcctccga taatttgaag
     1081 gtgtctttga ttggatcggt cacatatact ttatcgtctt ttgtcacgaa gatcgcttcc
     1141 gtcccgttat tcaattcgct ttcgacgtat tccagtcctt ttttcacacc cattgaaaag
     1201 actgctgtag acaacccgtc accatcaatt gatttatctg ttatgatcgt gacacttgcg
     1261 atatcattgt cgaacggata acccgttttt ggatcaaaca aatgatggta ttttttgcca
     1321 tctacttcta aataacgctc gtaaattcca gaggtaacta atgttttgtt ccgttcttta
     1381 atagaaccta aaacagatcc gcgagccatg ttcggatctt ggatgccgac tgtccagtct
     1441 tggttttcgc ctcttgggct gtggcctaag acatagacat taccgcccaa gtcaacgatg
     1501 gcagtggtca ctccttgttt tttcaagact ttcactactt cgtccgtaat gtaacctttg
     1561 gcgattgcac ctaaatcgat gatcattcct ttttctttta gataaacagt tttttctttg
     1621 tcatttagtt cgatcttatg ataatccact aatttcaatg cctgatcgat ttcttcttgg
     1681 gaaggttttc tggcgtcatc aaaaccgatt cgccataatt gtgtgattgc accaatcgcc
     1741 atatcgaatc cgccttgaga atcttggcta tattcgtagg cccgcttgac taacgtgtag
     1801 acgtcatcag aaacttttac tggtttgatc ccagcttgct cattcacttc atcgatctca
     1861 gaaccttttt gattgatcgt gattttatca ccaagttctt ttactcgatc gaaggcaggt
     1921 ttcaatgctg attctttgcc ttcatcatac acacggatac gaacgtaagt ccctaacaag
     1981 aactgttcat cagaatatgg atctttcaat atcttggtag ctgctgaact ttcagtggtt
     2041 gttttatttg ctgagttgct gcagccagtt agcaccataa aagagatgcc cagtgtgatt
     2101 aaggaaaata gtagtttttt ccgcatcttt ttcctctctt cattttgaaa ggtgagacga
     2161 ctgcctaatc tggctatcgt cccaccctcc aacttttatt agtatgatta tgtgattatt
     2221 tttctgttac gatattatct acttcgattg tttgagtgtc gcctttttca gctgcattca
     2281 ctaattgttg tgcgtacatg atgaatgaat gtgagctgtg tgtagcacct gtaacaactt
     2341 ctacattagc aggtgagcca tcttcttcgc ccattgcttt gacgaattcg tcgttcagag
     2401 tagggatgta agtttttggt tctgtaccgc tttttgcttt catattttca ttatattctg
     2461 tatcatcttg ttttgattta ccgtctttgt cgatataatc aaagtttgat tcagttactt
     2521 tgccgccagc aactgtcatt tcgaattgca cacggtagcc gttagagtag ttcttttctt
     2581 caagcttgta agtgccgtct ttcaaatcag caccattgtc gatttcgatt gtatctgtgt
     2641 taccagcttg agcagcttgg atcaattgtt gtgcgtagtt ttggaagctt tcagatgagt
     2701 gagtagctcc agttacgact tccacaccgc ttgcgctttg tgcttttaag aattggtcat
     2761 tcaactcagg aatgtatttt tctgggtttg tgccagattt tgcttccatg tttttgttgt
     2821 attctgcatc ttttgttttt gattcgccgt tttcattgac gttatcatat tttgattcag
     2881 tgattttgcc gtcttttaca accatttcaa agactgcacg gtaaccattt gaataatttt
     2941 tttcttctaa tttatatgtg ccgtcttgca aatctccgcc agctacaact tttgcagatg
     3001 attctgttgt gctttttgca gtcgaagatt gtgctgtaga tgattcagaa gaagagtttg
     3061 agcttccgtt gtcatctgcg ccgcaagcgc ctaacactag agctgaaaat gctaatacgg
     3121 caaatccagt tacaaaacgt tttttcttca ttttaatacc ccaccattcc atttctagtc
     3181 cacaattttt ctttgtgatt cattatacat tctaatcttt ttagcctata agtcaactat
     3241 ttttgataaa taacccatgt aatatttcaa acgttttctt tcagcaaatt tcgtgaaaaa
     3301 acgctttatt ttaaaatttg aaaaaaatcc aaaaggagtt agacaaaaga acagaacatc
     3361 tatataatat atggagattg tgtaataagt atcaaactaa aagaattatc atatattcat
     3421 tatatagata gtctataagg agcggatgat caatggcaaa gcaaaatatt gtcgttgtag
     3481 gagcaggata tgccggtgtt tcagctacaa aatacttggc aaagaaactg aaaaaagaag
     3541 atgtagcaat tactctgatc gatcgtcatt cgtaccatac tatgatgacc gagttgcatg
     3601 aagtagctgg cggacgtgta gaaccagaag caatccaata cgatctgcag catttgtttg
     3661 ctcgtcaaaa gaatgtacaa cttgtcacag acactgtaat cggtatcgat aaagaacgaa
     3721 aaatcgtcaa gaccaaatta ggttcctatc cttttgatca gctgatcctt ggaatgggcg
     3781 gagagccgaa tgactttggt acacctggtg tcaaagcaca cggattcacc ctttggtcat
     3841 ttgaagattc tgtaaggatt cgggaacata ttgagaagac agtagcaaaa gctgcattgg
     3901 aaccagatga tgaactgcgt aaagccatgc tgactttcgt tgtctgtggt tctggattta
     3961 ccggtattga aatgattggg gaactgatcg actggaaaaa tcgcctagcc aaagattata
     4021 aattagatcc aaacgaaatc acactgatgg tcgtagaggc aatgccgacg atcttgaata
     4081 tgttggatcg aaatgatgct gcaaaagctg aacgctattt gcgtaagcac aacgtggaat
     4141 tattattgaa tgcaccaatc gtagaagtgg ctgctgatca tatcaaattg aaagacggac
     4201 gcaacattcc gactcacaca ctgatttgga cagctggtgt caaaggaact tctgacgctg
     4261 ctgactttgg attagaatca gcacgcggac agcggttgat cgccaatgaa tatatgcagg
     4321 caaaaggcta cgaagataaa aacatctata tcattggtga tttagtcttt tatgaagaat
     4381 tcccaggaac accgacaccg cagatcgttc aagctgctga acaaacagga cacacagccg
     4441 cagcgaatat cgtggcttcg atcaaaggcg gagaaaagca taaatttaaa ggaaactatc
     4501 aaggatttat ggtttctatt ggtgccaaat ggggggtagc taacctattt gataagatcc
     4561 atttaagcgg atttttagct atcgtaatga aacatatcgt gaatttgaaa tacttctttg
     4621 atattcgttc tggttattat atgttccaat atttaatgca tgagtttttc catatcaaag
     4681 atgatcgcaa cgtgactcga ggacattctt ctcgttacgg caatgtctta tggagtgtgc
     4741 cacttcgtat tttctatggt ttagtttggt tcgttgaagc ttccaaaaaa atcattggga
     4801 atggtgaagt gttcaaacca agtacttggt ttggtgaagg atcatggttc actggaaaaa
     4861 gtgtgttccc ttggacatgg gaatacaatg taacagatgt tgccagtggt gcttctgctg
     4921 ctggtgatgc aaccacagct gccagcggag caggggcaag tgccggagaa gcagcgactc
     4981 aagctgcaca ttttggtttg agttatgcat acggtgaaga accaatggct gtgattgaga
     5041 agtcaccaga ctggttcaca aagatcatgc aagtgatcat gccaaacaat gatgtagcaa
     5101 tgttcttcca aaaattcatg gtattctttg aaattgcctt ggcattagca ttgattgctg
     5161 gattattcac atggctatgc agtgcgacaa cgattgtatt ggtgatcacg ttctgcttat
     5221 caggcatgtt ttactgggta aacatttggt tcatttttgt tgctttcgca ttgatgaacg
     5281 gttccggacg tgcgattggg ttggatcgct gggtaattcc atggctacaa cgtacactag
     5341 gtaattggtg gtacggtaga ccaagttccc agtacggcaa gaaataaata gataaagaac
     5401 gttatttgaa tgcaagattt caatagaaag gggctgagac aaaccgtcac agcctccttt
     5461 ttatgtaaaa ttaaaggaat aagatgcccg aga
//
LOCUS       DBNICI010000162         5448 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.166, whole genome shotgun sequence.
ACCESSION   DBNICI010000162 DBNICI010000000
VERSION     DBNICI010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.166"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            131..505
                     /locus_tag="AC1ZCC_002439"
     CDS             131..505
                     /locus_tag="AC1ZCC_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HIQ1630662.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="AC1ZCC_002440"
     CDS             complement(619..1248)
                     /locus_tag="AC1ZCC_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIQ1630663.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="AC1ZCC_002441"
     tRNA            complement(1382..1454)
                     /locus_tag="AC1ZCC_002441"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="AC1ZCC_002442"
     tRNA            1668..1741
                     /locus_tag="AC1ZCC_002442"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="AC1ZCC_002443"
     CDS             complement(1879..2043)
                     /locus_tag="AC1ZCC_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630664.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="AC1ZCC_002444"
     CDS             2338..3666
                     /locus_tag="AC1ZCC_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HIQ1630665.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="AC1ZCC_002445"
     CDS             3868..4701
                     /locus_tag="AC1ZCC_002445"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HIQ1630666.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..>5448
                     /locus_tag="AC1ZCC_002446"
     CDS             4717..>5448
                     /locus_tag="AC1ZCC_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HIQ1630667.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKN"
BASE COUNT         1837 a          839 c         1075 g         1697 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaat
//
LOCUS       DBNICI010000163         5395 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.167, whole genome shotgun sequence.
ACCESSION   DBNICI010000163 DBNICI010000000
VERSION     DBNICI010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.167"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(54..182)
                     /locus_tag="AC1ZCC_002447"
     CDS             complement(54..182)
                     /locus_tag="AC1ZCC_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630668.1"
                     /translation="MTLQEHQHLMRKLNQEYHEEMKPRLLGEQWRERQKKWLEIKK"
     gene            260..484
                     /locus_tag="AC1ZCC_002448"
     CDS             260..484
                     /locus_tag="AC1ZCC_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HIQ1630669.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            complement(481..636)
                     /locus_tag="AC1ZCC_002449"
     CDS             complement(481..636)
                     /locus_tag="AC1ZCC_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630670.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGEKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(793..948)
                     /locus_tag="AC1ZCC_002450"
     CDS             complement(793..948)
                     /locus_tag="AC1ZCC_002450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630671.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            1020..1250
                     /locus_tag="AC1ZCC_002451"
     CDS             1020..1250
                     /locus_tag="AC1ZCC_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HIQ1630672.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            complement(1247..1405)
                     /locus_tag="AC1ZCC_002452"
     CDS             complement(1247..1405)
                     /locus_tag="AC1ZCC_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630673.1"
                     /translation="MEEKNIKVPTLEEYTKIVVETDEQNPKTIAVITANDIESCEGFR
                     VRMTPRYD"
     gene            complement(1418..1684)
                     /locus_tag="AC1ZCC_002453"
     CDS             complement(1418..1684)
                     /locus_tag="AC1ZCC_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1630674.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            1884..2588
                     /locus_tag="AC1ZCC_002454"
     CDS             1884..2588
                     /locus_tag="AC1ZCC_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HIQ1630675.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            2675..3964
                     /locus_tag="AC1ZCC_002455"
     CDS             2675..3964
                     /locus_tag="AC1ZCC_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HIQ1630676.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            4030..5382
                     /locus_tag="AC1ZCC_002456"
     CDS             4030..5382
                     /locus_tag="AC1ZCC_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIQ1630677.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALMKELNE
                     IEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNITVNFLI
                     "
BASE COUNT         2000 a          816 c          909 g         1670 t
ORIGIN      
        1 aatctcctta ggtataattt ttggtttgtg actcattgct ttggacggct gagtcacttt
       61 tttatttcta accacttctt ctggcgttca cgccattgtt cccctagcaa tctaggtttc
      121 atttcctcgt ggtattcctg atttagtttt cgcatcaagt gttgatgctc ttgtaatgtc
      181 atttgatccc tcctttcgtt tattgaattt ctccatttct atttgataaa atatttctat
      241 agaaatggag gtgaaaacta tgggaaaaaa tcaacatgtt gtacctaatt ccaatggtgg
      301 ttggaatgtc aaaggtgctg gaaattctaa agcgactgca catactaata caaaatcaga
      361 agcagtaaaa atcgctagag agatttctaa aaatcaagga tctgagctat ttattcatgg
      421 cagagatgga aaaattcaaa gccgtgatag tcacggaaac gatccacacc caccaaaagg
      481 ttaatcatat ttaggagtta atcgaatacg ataaccgtca gcaggagttg catcggtcaa
      541 tgtaacttct gctacttttt ccccttcttc tgtttcaata attaatcttg tgtaattgtt
      601 ctcattcaaa atccctattt ttgattcttc cttcatgtca tccctccttc gtctctcgcc
      661 gtcaccacct ccaagaagag gagttgccaa aatgaagaaa cgtggacttt ggcaaacttg
      721 aaaggagagc agcaactcct cttttcggaa gtggcgagtg tgtgatataa tattattgtt
      781 ctatggtgcc cactacttgc ttgccggctg tgtgggcttt tctttttgtt caattatggt
      841 tgccaccaca gtaactcccc aatgtctaga acgtattcta gccatttcat ctgctaatct
      901 ttgaagatta ggtgtgccat tcaaagtgat ttttggcttg ttactcacaa tctcacctcc
      961 atcagtttgt tttcttttca cctcttaata gaattaactt gaaagcgagg tgaattgtta
     1021 tgggtaaaaa tcaatgggta tctcctagaa atggcaaatg ggctgtacgt ggtgaaggta
     1081 attccgaaga tacaaaacta tttgataata aatcagatgc tgttgagtat ggtaaaaaga
     1141 tttctaaaaa acagcaatca gagtttattg tccaaaaaaa gaatggacaa atacaatcaa
     1201 aagatagttt tggaaacgat cctatacctc cacgtgataa agaacattaa tcatatctag
     1261 gggtcattcg aactcgaaaa ccttcgcatg attcaatatc attcgccgta ataactgcga
     1321 ttgttttcgg gttctgctcg tctgtttcga caacaatttt tgtatattct tcaagagtcg
     1381 gtacttttat attcttctct tccactattc acacctccta gctgacttgt tttgtttcac
     1441 ctgttattga tttcggtttt tccgaatcag ggtctaaaaa aatatcggaa actctaacac
     1501 ccaatgcctt agcaattttt tctagagtgg cgtactttcc gttcctcatt ttatctacat
     1561 cgttttcgta aataaatatt gttcttgatg tcactttgct tttccttgac agttcttctt
     1621 ttgaataacc ctttaaagct cttaattgtc tcaatgtata tttttctttc ttcgcggtat
     1681 ccatatttta ttcgcctcct ctctttaact ttctgactaa attatatact tcggtttttc
     1741 cgaagtcaag tgcatatttc agttttcctg aaatcgattt ctattttgcg taaatacttc
     1801 tttaaaaaca ctaaaatatt ttctaagaaa cctgttacat tttttccgaa acggtgttac
     1861 aattaactca aggaggtgtt actatgttcg ctgaaaatct aaagtttctt agagaaaagt
     1921 ataatattga tcaacaagaa ctagccgaaa aactaggaag aaaaagttca tcctcaataa
     1981 gcgaatggga aaaaggaaaa tatacaccaa aattaaaaac cttaaatgag atagctaaga
     2041 tattcaatgt tgatatagat gatttgatga atattgattt atctatatct aatcagatat
     2101 ataatatcct tcctatatat aatcagctta acgacgaaaa taaagttgct acctatgaat
     2161 ttgcaagtaa tcgattagaa gaacaaaaac aagaaaatgt cctagatttt cctaaaaaag
     2221 aaaaaatccc aactgttcac aattcggctt cggctgccaa tccaacagaa cttacttacg
     2281 gtgatacggt tgtcgaagag gaagaatttg agagagttcc aagtgctgct gacttcgcag
     2341 tacctattat aggcgattca atggaacctg ttatcaggaa tggacaattc gtattcgtta
     2401 aagaacaacc ggacgtagaa gatggggaaa ttgctattgt ggaattaggt ggtgacggag
     2461 ttacatgtaa agagatatat aaagattacg aaaatcaaac aatcatttta cgttctataa
     2521 acgacttata cgaagataga attgtatcgc cagaacagat tagaattatc ggaaaagttg
     2581 tattttaaaa aaattcccat gccggagccg tcaacttcgg taagggttta tatgattttg
     2641 acaattattt tataaaagaa gcgaggcgtt ttgaatggga gtactattgt ttttagttgg
     2701 aatagtttta ataattatgg gtagtagcaa attatctgaa gctaaaaaat taaaagataa
     2761 aacttctgcc ttagagagta aggtaaagga atattctcaa gaattaaatg ctagggaaac
     2821 gtttatatct gaaaaagaga aaaacataag taaattgaat aaaaacttgt caacgctaga
     2881 aaacaaaatt gatattctta atgctgaaag ccagcggtta gacgatgaaa ttaatcaaaa
     2941 atatgacaca tctctggtca ctatctataa atcagtaatt attccgaata tagatgaaac
     3001 ttcttcatcc gaaataaaaa acaaactttc tttattaaag attaaagaaa aggattttat
     3061 tttagacggt gcaattaata ttccttctta tttgaataat agagaagcta ataatattaa
     3121 aaagaaaatg cttataccat tagaatcaga aataactgga cttctcaata aacttactat
     3181 ttctaacgta gattcaacta gagaaaaaat tataagaact tttgataaaa taaacaagct
     3241 ttttaaaaac gatgaagcag aattcaaaaa agaattactt gaattaaaac ttcaagaatt
     3301 agaactcaac tattcataca tagttaaagt taacgatgaa aaagaacagc aaaaagcaat
     3361 aaaagaacaa atgattgaag aagaaaaggt aagacgtgaa atagaacgtg aaaagaaacg
     3421 cattgataaa gaagaacgac agtttaactc agagataact aagttaatgg catacatgca
     3481 aaaagctagt gctgatgttg aaaaagaact ctatgctgat aaaattaaag aactggaaga
     3541 gaaattaaga gaactagaaa ctgtcaaaga agatgtattg caaagagaac ttaatactag
     3601 agctggctat gtttatgtca tcagtaatat cggatctttc ggtgaagatg tctataaaat
     3661 aggaatgact agacggttag aacctatgga tagaattaag gaacttagta gcgcttctgt
     3721 accttttgaa tttgatgttc atgcaatgat attttcagaa gatgcaccaa cattagaaaa
     3781 tcaactacat tcgcatttta gaaaaaacga agtcaataaa gttaatcaac gaaaagaatt
     3841 ctttaaagta tctcttgatg aaattgaaaa agttgtttta gaaaattata acggaactgt
     3901 atcttttact aaactagctc aagctgaaca atacagaaga tcattagaat taacagatgt
     3961 gtagtaaaaa gattagcctt cgggcttttc ttttacaacg ataagaacat acattcgaaa
     4021 ggagataaca tgaaacgagt agcattatat atgagagtat caactgaaca acaagccaaa
     4081 catggagata gtcttagaga acaaaaagaa accctctatg aatatatcga acaacacaag
     4141 gatttgaaag tagttaatga atatgtagac ggtggtattt ctggtcaaaa aataaatcgt
     4201 gatgaatttc aaaaactatt acaagatgta aaagaaaata aaatagatct aattttattt
     4261 acaaaattag atagatggtt tagaaatcta cgacattatt taaacactca agaaatttta
     4321 gaaaaacaca atgtttcttg gaatgctgtt tctcaacaat attatgatac aactacagcg
     4381 tatggcagaa ctttcattgc acaagtaatg agttttgctg aattggaagc ccaaattgat
     4441 tcagaacgca tcaaagctgt tatggcaaat aaaattgcac aaggtgaagt agtaagcggg
     4501 aaaactccgt taggctactc tatagaaaat aagaaattag taataaatga tgatgctcct
     4561 atcgtgatag atatattcaa ctactttctt tctagtggta gtttaagaaa aactgtttat
     4621 tatttaggct ctcagtatgg aatcgttaga gattatcaaa gtgttaaaaa tatgttgacg
     4681 aacaaaaaat acattggtga attaagaaat aataaaaatt actgtcctcc tattattgat
     4741 aaaaaacttt tttatgctgt tcaaaaagcc ttacctaaaa atttaaaaac aaatgcaaaa
     4801 cgtgattaca tttttaaagg tttattaaaa tgttctgact gtcagggatc agtagctggt
     4861 caaactatta aagcaagata taagaaaaaa gacggtacag aatctatata cgaaaggact
     4921 tgttatagat gtgtaaaacg aagaaataat aaattacgtt gcacaaataa gcgcgctttt
     4981 tacgaaaaaa atttagaacg ttatattttt gaagctacta aacaaaaatt tgaacaaata
     5041 caaataaact attcaaaaaa acaaccaaag attattaaaa agaaaaatag taagaaaaat
     5101 attgagaata aattagatag attgaagaaa gcctacttga atgaagttat agacttagag
     5161 gaatacaaaa aagatcgaga agctttaatg aaagaattaa atgaaattga agtagagcca
     5221 gctaaaatag atataaaaaa tgtcgaattt attctatcaa aagaattcga tgaaatttat
     5281 aaagaatctt cggaagaaga aaaaaatgca ttatggcgtt caattatcga taacataata
     5341 gtatttccag atggcaatat aacagtaaat ttccttatat aatttatgtg tacta
//
LOCUS       DBNICI010000164         5339 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.168, whole genome shotgun sequence.
ACCESSION   DBNICI010000164 DBNICI010000000
VERSION     DBNICI010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.168"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(19..414)
                     /locus_tag="AC1ZCC_002457"
     CDS             complement(19..414)
                     /locus_tag="AC1ZCC_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630678.1"
                     /translation="MKNKGQIWYIGYIVSLFLIVTILVTDFPKMVDIGLAILFSAVFG
                     VSHVQILHDKMIKNDADYKINVMDERNIIIKEKAGNITNMFNTILLGVATVIFICLDY
                     IIPAIIVGSIIAIQPIILIIISSVLEKKM"
     gene            complement(404..616)
                     /locus_tag="AC1ZCC_002458"
     CDS             complement(404..616)
                     /locus_tag="AC1ZCC_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIQ1630679.1"
                     /translation="MKNRLEELRKQRGIKQEDLASALEVSRQTIGSLENGRYNPSIIL
                     AFKIARYFQMSIEEIFIYEEESEDEK"
     gene            1688..2290
                     /locus_tag="AC1ZCC_002459"
     CDS             1688..2290
                     /locus_tag="AC1ZCC_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiJ-NTD4 domain-containing protein"
                     /protein_id="HIQ1630680.1"
                     /translation="MEDIILRDRLTDPLKNSLANVIYDILREADKVYSNYNGKVSWVI
                     YSFLIKEFFKEQINNSYELGYHFTVYYEDIIKRLEDLEWYAYYDLLEVFASAKILDKK
                     KREEVNKILESENSGYRLSESNEFIPITDDISIKSINAVSESPFNYAKNHIHKSLTYI
                     SEKENQDYNTVVREAISAVESCLIEFSGLPANKKILWELL"
     gene            2645..2881
                     /locus_tag="AC1ZCC_002460"
     CDS             2645..2881
                     /locus_tag="AC1ZCC_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIQ1630681.1"
                     /translation="MNIKKRKIREVGNSIVVTLSKESLLQKGLKPGDTIFIDEDKMME
                     AIVKEESKIDLEIDMYVNQALSEYDAAFKELVEK"
     gene            2878..3291
                     /locus_tag="AC1ZCC_002461"
     CDS             2878..3291
                     /locus_tag="AC1ZCC_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HIQ1630682.1"
                     /translation="MIYLSEKDIVKLNVIQIKKYSPRETIGVIDKNALSMAVNQPKQH
                     IFGKDLYPDKFSKAAVLLINLIKKHPFHNANKRTAFLAAYVFLKLNGYSLKMKNQEVV
                     EFVVEIATYQGDFDDLKEATTDILKKNSTKTDSKK"
     gene            3834..4760
                     /locus_tag="AC1ZCC_002462"
     CDS             3834..4760
                     /locus_tag="AC1ZCC_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HIQ1630683.1"
                     /translation="MTGRNPKVFISYSWENKENKEWARELADKLLENGIDAYIDQYDL
                     SLGDRLPQFMEQQISDSDYVLIICTPEYKKKANNRTSGVGYEGHIISAELLSGKNERK
                     FIPIIKEGRASEVLPTFLAGKLGIDLSDSQHYDENLDDLITTIFGVKRKPALGDIPRH
                     IKGSPLLPIEHNENKDIEILGIITDKVTVPKMDGSRGSALYKIPFRLNRVPSQLWKEL
                     FVRNWNSPPRYTTMHRPGIASVVDDEILLNGTTIEEVREYHRETLKLCIEKSNTQEKK
                     ILLEEEQKKERENERVRSHKESVQKLADELEF"
     gene            complement(4757..>5339)
                     /locus_tag="AC1ZCC_002463"
     CDS             complement(4757..>5339)
                     /locus_tag="AC1ZCC_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIQ1630684.1"
                     /translation="SIDQNEQRQIEEMRKFGAEKIFIEKQSGATITHRPIFQEVLDFV
                     RDQDIFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITNLPIMAEAIGNPLLDKFIKD
                     LIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANTKDPQRRLVYRSIV
                     QDQENGVAISKIATDYNVTRQTVYRIKKELTIN"
BASE COUNT         1908 a          760 c          902 g         1769 t
ORIGIN      
        1 ttgtatgaac ctttatttct acattttttt ctcaagcaca ctactgataa tgattaaaat
       61 aatcggctgg attgcaatga tacttccgac aataatagct ggaatgatgt agtctaaaca
      121 aataaatata actgttgcca cccctaacag tattgtgtta aacatatttg ttatatttcc
      181 cgctttttct ttgattatga tattccgttc gtccataaca ttgattttgt agtctgcatc
      241 atttttaatc attttatcat gcaaaatttg aacatgagaa acgccaaaaa cggcactgaa
      301 taaaatagca agtccaatat ctaccatttt aggaaaatct gtaaccaaaa ttgtcacaat
      361 caaaaacaac gaaacaatat aaccgatata ccaaatctga cccttatttt tcatcttcac
      421 tttcctcctc atagataaaa atttcttcaa tgctcatttg aaagtacctt gcaattttaa
      481 atgccaatat aatagaggga ttataacgtc cattttctaa agaacctatt gtttgacgag
      541 atacttcaag agcagaagct aaatcttctt gtttaattcc tctttgtttt cgtaattctt
      601 ccaaacgatt tttcacaaca tgctcctttc taaaaaatgg aaagtttact ttcctttcgt
      661 gcttttataa tactcctgcg agcacaaaat gtcaaggaag ctttccatat aaattttgat
      721 tttaatgttt tagttactgt ttttatgaca catgaacaca cttattctta tttcctctta
      781 tctgctatca acagaaaaga aaaagccctg tcaagagccg attagatcaa gccagtcgag
      841 aaacgatgtt agaccaaatt gattttgggt aatgaatgtt tagaacttag tagtcatcca
      901 aaaacgacta ctttttttgt tgaatcgtcc aaggacgatt cgatgctttt gacttgtaag
      961 aacaggattg aatgaaacca tactgttcgc atatttttag tggatgaaca aacaaaatac
     1021 gagagatttt ttgttcgttc atccatggtt ttagaaaaaa gagggacgat tttggaagaa
     1081 gaaaatcgtc tctttttttt cttctttttg tatgacaaaa agaaagatct tttgcccatt
     1141 ttatttttat aaaatgggta ggtggcgttc gcgtaaagca aatcgataca atccaatggt
     1201 tttggcgaat cagatttacc attctaggct tgtttaatac agacatcgat tgttattttg
     1261 agaatttata tccttattgg caatgaaatg gatattctcc ataccaatag aaatcttcaa
     1321 gataattttc ggaaatatat aagagttaat caattatgat catatagtag gtaaaaatta
     1381 ggacagcctt caattatatg ggaaaccaat ataatcaagt aattattcgg gtaacacaaa
     1441 tactagagta ctcatttctt ataactgttt tacacctatt agggatcagt attattaata
     1501 gatctagata aattaatacg taattatata ataatttatg tttgtgccat taaacaatct
     1561 taacagatat ttcattttat ttatgtaaaa caacacgaat aattattgct tcgtaaagtt
     1621 atatgagtat aagttaattt actggaggta gtgcaatgct tttttctgaa agatttggtt
     1681 ttaaaaaatg gaggatatca tactgagaga tagattgaca gaccctctta aaaatagttt
     1741 agcaaatgtg atttatgata tattacgaga ggctgataaa gtttatagta attataatgg
     1801 aaaagtctca tgggtaatat attctttttt aattaaagag ttttttaaag aacagataaa
     1861 taatagctat gagttgggtt atcattttac tgtttattat gaagatatta tcaagaggtt
     1921 agaagacttg gagtggtatg cttattatga tttattagag gtttttgcaa gtgctaaaat
     1981 cttggataaa aagaaaagag aggaagtaaa taaaattctg gagtcggaaa attctggtta
     2041 cagattaagc gaaagcaatg aatttattcc aatcacagat gatatttcta ttaagtctat
     2101 aaatgctgta tctgaatcac catttaatta cgcaaaaaat catatccata aatctcttac
     2161 atatataagc gagaaggaaa atcaagatta taatactgta gtgagagaag caattagtgc
     2221 tgtcgaaagt tgtcttatcg aattttcagg attaccagct aataaaaaaa tactttggga
     2281 actgctataa aagcaattaa agacaaatcg aataacaatg tcgatcttac atttttaaaa
     2341 ccatttgaga caatgtatgg tttggcgagt aataatggaa tacgacatgc agaaaatgaa
     2401 aaaacaattg tctcagattt acctgatgca gttttggtgt taacaacatg ttcagccgta
     2461 attaattatt tgggagtaaa aatagcatat gactccaata aatcatgaaa agttaaaatt
     2521 catccaagag ctacattata actattaatt tccaactctt taaaagttta gtaattgaag
     2581 gaagtaataa gggtgattga acaatacgga tatacggtgt atacttatcg taggagttga
     2641 tgatatgaat attaaaaaac gcaaaatcag agaagtaggt aattcaatag ttgtaacttt
     2701 gtcaaaagag agtttattgc aaaaaggttt gaaacctgga gatactattt ttattgatga
     2761 agataaaatg atggaagcta ttgtaaaaga ggaaagcaag atagatcttg aaattgatat
     2821 gtatgttaac caagctttat ctgagtatga tgcagctttc aaggaattag ttgaaaaatg
     2881 atctatctaa gtgaaaaaga tattgtaaaa ttgaatgtta ttcaaataaa gaaatattca
     2941 ccgcgagaaa caatcggagt cattgacaaa aatgcgctga gtatggctgt aaatcaacct
     3001 aaacagcata tttttggaaa agatctgtat cctgataaat ttagcaaagc agctgttctg
     3061 ctaataaatc tgatcaaaaa gcatccattt cataatgcta acaaacgtac agcattttta
     3121 gccgcgtatg tttttctcaa gctaaatggc tattcactaa aaatgaaaaa tcaggaagta
     3181 gtagaatttg tagttgaaat tgctacgtat cagggtgatt ttgatgactt aaaagaggca
     3241 acaacagata tactaaaaaa gaattcaaca aagaccgatt caaaaaagta gaagcaaact
     3301 agggattcag ttaaatctga agatttagtt tgttctttat tttagtttac ataagataca
     3361 ttatgcgaag ttcattattt taaaagctta aataataaac ttttttataa taccaattta
     3421 tttatgtaaa ccaatgacct taatcgctta agcaagcatg ttatgtaata ctctactctc
     3481 tccaaaacta atgactatct attttcaaag aagctagaat gattatctga tcaaatgata
     3541 atttatattt aattcctggt ctactgctct tcttttaatc aattaatgtc taacgtactg
     3601 tacacgataa agtaggaaga cttattttca acttcggatt cattttttta attcggtgaa
     3661 aatcactaaa ttaagagttt atccaagttc attttagctg agaatacgct tgaaaataat
     3721 tttgatgaat tttcctgaat taaaatttat taacttgttg agtgatataa ttgtaatgta
     3781 ttaccaaata gttagcactt agattagtga tggaaaggaa tagtgagaac aagatgactg
     3841 gtagaaatcc caaagtattt atatcatatt catgggagaa taaggaaaat aaagaatggg
     3901 ctagagaact agcagacaaa ttacttgaaa acggtattga tgcatatata gatcagtatg
     3961 atttaagttt aggtgataga ttacctcagt ttatggagca gcaaatatcc gattcagatt
     4021 atgttctaat tatatgtaca ccagagtata aaaaaaaggc aaataatcgt acaagtggtg
     4081 ttgggtatga aggtcatatt atctcagcag aattgctttc tggaaaaaat gaacgtaaat
     4141 ttatcccaat aattaaagaa ggacgtgcaa gcgaagtact tcctactttt cttgctggaa
     4201 agcttggaat tgatttatca gatagccagc attatgatga aaacttagat gatttaatta
     4261 caactatatt tggtgttaag agaaaaccag cattaggtga tatacctagg catattaaag
     4321 gcagtccttt actacctata gaacataatg aaaataaaga tattgaaatc ttaggaatta
     4381 tcactgataa agtaactgta cctaaaatgg atggctcaag gggttctgca ttatataaaa
     4441 ttccatttag attaaataga gtaccaagtc agctttggaa agagttgttt gttagaaatt
     4501 ggaattcacc accacgatat acaacaatgc atagaccagg aattgcttct gttgtagacg
     4561 atgaaatttt acttaatgga acaactattg aggaagtccg ggaatatcat cgggaaactt
     4621 taaaactttg cattgaaaaa agtaatactc aagaaaaaaa aatattgcta gaggaggaac
     4681 aaaaaaaaga gagagagaat gagcgagtca gatcacataa agaaagtgtt cagaaattag
     4741 cagatgaatt agagttctag ttgatagtca gttctttctt aattcgatac acagtttgtc
     4801 tagttacatt atagtctgtt gctatttttg aaatagcaac accattctcc tggtcctgaa
     4861 cgatgcttct ataaacaagg cgacgttgag gatcttttgt gttcgcactg tataacttag
     4921 gacgtccttt atagacgccg tttgctttag caatctttat cccttgtgcc tggcgtcgtt
     4981 ttgattcagt acgctcttgt tcagcaatca tggccaagat ttgaatgatt aggtctttga
     5041 taaatttatc caggagagga tttccgattg cttcagccat gatcggtaaa ttagtgataa
     5101 taagtcgcac atttttcttc tttagataat ttactgaatc gataatctcg tcataattac
     5161 gtccaagtcg atcgatagcc tctacaataa aaatatcttg gtctctaaca aaatctagaa
     5221 cttcctgaaa aatcggtcga tgagtaatag tcgctccaga ttgtttctcg atgaaaattt
     5281 tttcagcacc aaattttctc atttcctcga tttgacgttg ttcattttga tcgatcgat
//
LOCUS       DBNICI010000165         5311 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.169, whole genome shotgun sequence.
ACCESSION   DBNICI010000165 DBNICI010000000
VERSION     DBNICI010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.169"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(114..791)
                     /gene="phoU"
                     /locus_tag="AC1ZCC_002464"
     CDS             complement(114..791)
                     /gene="phoU"
                     /locus_tag="AC1ZCC_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIQ1630685.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            complement(804..1562)
                     /gene="pstB"
                     /locus_tag="AC1ZCC_002465"
     CDS             complement(804..1562)
                     /gene="pstB"
                     /locus_tag="AC1ZCC_002465"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIQ1630686.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            complement(1578..2384)
                     /gene="pstB"
                     /locus_tag="AC1ZCC_002466"
     CDS             complement(1578..2384)
                     /gene="pstB"
                     /locus_tag="AC1ZCC_002466"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIQ1630687.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            complement(2399..3283)
                     /gene="pstA"
                     /locus_tag="AC1ZCC_002467"
     CDS             complement(2399..3283)
                     /gene="pstA"
                     /locus_tag="AC1ZCC_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIQ1630688.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            complement(3283..4203)
                     /gene="pstC"
                     /locus_tag="AC1ZCC_002468"
     CDS             complement(3283..4203)
                     /gene="pstC"
                     /locus_tag="AC1ZCC_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIQ1630689.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            complement(4318..5175)
                     /locus_tag="AC1ZCC_002469"
     CDS             complement(4318..5175)
                     /locus_tag="AC1ZCC_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HIQ1630690.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
BASE COUNT         1517 a         1186 c          935 g         1673 t
ORIGIN      
        1 gaggagctat tttttgtttg actgttctta ttacttgatg cagaacagct ttttcacaac
       61 cgctgatttc acaacctctt ttcctaaaca aacggcataa tcacagcttc agtttagaaa
      121 atctcattgt ggttcgtatt taactcagtg attttccctg tagccaagta gacgatccat
      181 tcacagatgt ttgtcacata gtctccgatc cgttctagat aactggcaac attcaagtag
      241 tctgttccac tgatgactgt ctcaggattt tcctgcatat ttttgattgt tgcattatag
      301 attcgattga aatagtcatt gactctttga tccatattgg cgatcgtacg tgcgtctttt
      361 tcgtctgttt tgacataagc aactaggacg ttgtccacca tttttttcac ataatcggac
      421 atatcggaga tctctttttc gatttccggt attctcgtct gcccttttac gcgaatcgtg
      481 gatttagcaa cagacaccgc atgatcggcc atacgttcta aatccgagct agctttcatg
      541 accgtaatga tcatacgcaa atcagttgta actggctgtt gcaatgcgat catttcaaaa
      601 cttttcttct ccaagcgtgt ttccatgtta ttgatattta tatcgttttc gataacttct
      661 tgggctaaga ttttatcgtg tttgatgtat gcacgaactg acttgtgcac ggcgctgctc
      721 accatcatcc ccatttcgta gaactgatta tggagattca gcaattcttc ttcaaattgt
      781 gtacgtagca ttccgtttcc tccttatcca aatcgacctg aaatataatc ttctgtctct
      841 tgtttttctg gattcatgaa tatcttcttc gtatcgttgt attcgatcaa gtgaccatct
      901 aaaaagaatg ccgtcttatc tgaaatacgt gaagcttgag acatattatg ggtcacgatg
      961 atcatcgtat accgctctct taattctaac agcatacttt cgattttccc agtagaaaca
     1021 ggatcaagcg cacttgttgg ttcatccaat aagatgattt ccggattaac tgccaaaaca
     1081 cgtgcgatac atacacgctg ctgctgccca ccggataaag ataaagcact tttatgcaat
     1141 ttgtctttca catcatccca aacagacgct gccttcaggc tttcttcaac tacttgatca
     1201 aggacttttt tatctttttc cccttttaat ttcaatccat agatgacatt ttcatagata
     1261 gaaaaaggaa atgggtttgg ctgctggaat accatgccga tttcttttct tagttcaaca
     1321 acatctgttt ttggaccata gatgtctttg tctttatata aaacacttcc ggtgatcgtt
     1381 acattcggaa tcaggtcatt catccggttc aaagaacgca gataagtgga tttcccgcaa
     1441 ccagaaggac cgatcatcgc agtgatttcc ccttgattga aacttagatt gatccctttt
     1501 agtgcttctt tctttccata atacagatga agatctttag atgaaataat ttcttttgtc
     1561 atgttgaggc ctccgtctta accaaaatga ccggacacat aatcttcggt tgcctgaatt
     1621 tttggtctgg taaatatctt gttagttttg tcatattcca ttacttttcc tagatagaaa
     1681 aaggcagtat agtcgctgat acgagctgct tgttgcatgt tatgcgtcac gatgatgatc
     1741 gtatagtctt ctttcaagtt tacaagtgtt tcttcaaccg ttcctgtcga aatagggtcc
     1801 aaagcacttg ccggctcgtc tagcagcaag atatcaggtt tcatcgcgat cgcacgagca
     1861 atacataaac gttgctgttg cccgccagat aaagccaagg cacttttgtc caagctgtct
     1921 tttacttggt cccacaaggc agcttgtttc aagcttgttt caacgatttc atctaatttc
     1981 tttttgtttt tttcgccgtg ctgtttcaaa gcaaatgtaa tattgtcata aatcgacttg
     2041 ctgaacggat taggacgttg aaaaaccatg ccaatccgtt tgcgcatctc ataaacatcc
     2101 acttgaggcg cattaatatc tacgccttga tacatgattt tcccagtgat tttcgtatta
     2161 gcgatcccat cattcattcg attcaacgaa cgaagatacg tagattttcc gcagccggaa
     2221 ggaccaatca gtgcagtgat cttatttttt tcaaattcca gatccactcc tttgattgct
     2281 tcattgtcgc cataccagac atgtagatca tccgtatata gtgctacttc tttctctttt
     2341 ggcatggtga tgacatttgt ttcattccag ttatattttt tcattttgct tcttctcctt
     2401 aggctgaagt catttttcgg tataatcgtt tgccaatcgc acgagcgccg aagttgaaca
     2461 gtaaaacagc aatgatcaac acggcagaag cgccagccga aacagaaaca ccatcaggca
     2521 tcgtgccttc tgtattgatc ttccaaatat ggacagctaa tgtttcagct tgacggaaaa
     2581 tgctgatcgg gcttgaaaca cttaatggat tccagttcgt aaaatctaac gcaggtgcac
     2641 tttgtccagc cgtatagatc aatgcagctg cttcaccaaa aattcttcca gaacttagga
     2701 tcacaccagt caaaatccct ggtgtcgctt ctggaaccac cacttttatc actgtttccc
     2761 agcgtgacaa acctaaggcc aatcctgctt ctcgttgtgt gtaatggacc gcttgtaaag
     2821 actcctcaac tgttctagtc aaaagaggca agttgaatac agtcaaagcc aatgcaccag
     2881 aaataatcga gaaaccatac tggaattgaa taacgaaaac caagaaaccg aataaaccga
     2941 caacgacaga aggcaacgag ctcaagattt caatcgacgt gcgaatcaca tctgttagcc
     3001 agttttttcc agcatattct gaaagataga tacccgcacc taaagaaata ggaaaactga
     3061 tgatcatcgt gatcaaaagc aaataaatcg agttaaatag ctggatcccg attccaccgc
     3121 cttcttggta ggctttggaa ggttcggtca agaaggacca tgaaatatgc ggtattcccc
     3181 gaactaagat ataaagtagt aaagctgcta aaatcaatac gatgatcccc gaaactatat
     3241 ataaaacacc tgtagcaatt ttatctgagc gttttgcgtt cattatttca gctctccttt
     3301 cttcccaatc atacggatca cgatattaaa gaataatgac atcaatagca agatcagcgc
     3361 taatgaccat aacacattat tttcaactgt tcccatgatt gtatttccga ttcccattgt
     3421 taagatggac gtcaatgtag aagcaggagt agtaagtcct gttggcatca atgctgcatt
     3481 accgatgacc atttggatag ctaaggcttc accaaacgca cgagccatac caaaaacgac
     3541 tgctgttaaa atcccgggca cggctgctcg tagcacgact ttgtagattg tttgccatct
     3601 tgtggcacca agcgctaatg aagcttcccg atagtgccgt ggcactgcct ttaatgcatc
     3661 aacagtcatc gttgtgacag tcggcaagat cataacgaac aacacaaatg taccagctaa
     3721 gataccaaac cctgtcccgc caaaaatact tctgacaaaa ggcacgataa ctgataaacc
     3781 gataaaacca taaacgacag aaggaatacc tacaagcagc tcgatgaccg gctgtaagat
     3841 cttcgttccg tttttaggag agatctccgt catgaatact gctgcaccaa tggcaaatgg
     3901 cgtggcaacg atagcagata aaaaggtcac aataaacgaa cctgtgatca taggcaatgc
     3961 ccctaccatt ggtttcccat ttgctccgat ttggcttgga ttccaatctg taccaaacag
     4021 aaaatcaaat acattgatct tatcagtaaa aaaggtggcc aatcctttac tagctacaaa
     4081 ataaaaaata gataatacaa ccaacacgat cagcgcgata cataaaaaac tgatgaattt
     4141 tccacgctgt tccatttttg aacgttttga ttttgttaat aatacttttt tcacatcttc
     4201 cacagatgac tcccccttaa tcaggtttaa catactcact cagtgtacaa attgaacatc
     4261 aatttggttt taataacatg taaaagttta gctaagtttg taaattttgt gtaaagatta
     4321 ctctttaacg atatttcctt gccaatcacg ttcgactttc atatgagaaa cagggatata
     4381 acctagttct ccaacgatgt tatcttgtac ttcatcagat aagatatagt ctaaaaattc
     4441 ttttgtcagt ttttttggat gatctcttgt gtacatatgc tcatatgccc aaatcgtcca
     4501 tttgttcgtt gtcacatttt catctgtcgg ttctacaccg ttaacattca acgtatctac
     4561 atcatcggaa acataagaaa aagcaacata gctgattgca ccgggtgtgt cggcaacgat
     4621 ctggcgcacc attccgcttg aatcttgttc ttgtgcctga acagctgttt ctccgtccaa
     4681 tacccatttt tcaaacgtgc tacgggttcc gcttcctgaa gcgcggttca atataactac
     4741 tggctgatct tttccaccga cttcactcca gtttttgatc tcgcctgtaa atagtttttt
     4801 caggttttcc atactgatgt cttttacgcc aatatccttg ttcacaatag gagtgatccc
     4861 aacaactgct actcgatgat cgactaatgc tgaggcatcg atccccgatt tttcttccgc
     4921 aaacaaatca gagtttccga tgtcgactgc tcctgactgt acttgagaca aaccagttcc
     4981 gcttccgccc ccttgtacat tgatgaattt tccaggattt tctgtctggt aagtttcagc
     5041 aactgtttct actaaaggct gtaaagcaga agagccaaca gccgtaatgg attctcctcg
     5101 atcaatccac tttgaacagc ctgataaaag aatggctaat cctattattg gtaagaataa
     5161 tttaagtttt ttcattagaa atctccttta atcctaaagt atgtttattt tatcattttc
     5221 attacttgat gaaaacgaat caaaatcatt aaataacaaa ataaattctc ataattgtaa
     5281 aaaaaactat ctcattaaaa gtttctatgc t
//
LOCUS       DBNICI010000166         5309 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.170, whole genome shotgun sequence.
ACCESSION   DBNICI010000166 DBNICI010000000
VERSION     DBNICI010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.170"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(71..1219)
                     /gene="nagA"
                     /locus_tag="AC1ZCC_002470"
     CDS             complement(71..1219)
                     /gene="nagA"
                     /locus_tag="AC1ZCC_002470"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HIQ1630691.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1295..2131)
                     /locus_tag="AC1ZCC_002471"
     CDS             complement(1295..2131)
                     /locus_tag="AC1ZCC_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1630692.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2315..3532)
                     /locus_tag="AC1ZCC_002472"
     CDS             complement(2315..3532)
                     /locus_tag="AC1ZCC_002472"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIQ1630693.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3681..3860)
                     /locus_tag="AC1ZCC_002473"
     CDS             complement(3681..3860)
                     /locus_tag="AC1ZCC_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630694.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3915..3998
                     /locus_tag="AC1ZCC_002474"
     tRNA            3915..3998
                     /locus_tag="AC1ZCC_002474"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3949..3951,aa:Leu,seq:gag)
     gene            complement(4238..5309)
                     /locus_tag="AC1ZCC_002475"
                     /pseudo
     CDS             complement(4238..5309)
                     /locus_tag="AC1ZCC_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1653 a         1082 c          838 g         1736 t
ORIGIN      
        1 ttactagaac acaaaaaaaa ccttaaaacc aaacttaaga agtttagttt taaggttaac
       61 ttaaattata ttatgcgtgg aaacgttcca cgccatctag ataagttgct tccaatgtca
      121 tatcagggtt caatacgata aagtctgcat cgcgtccttg agcgattttt ccacatgtat
      181 catcgatttt acagctgatt gctggaatca gactagccat catgactgct tgttctggtg
      241 ttgctaatcc ccagtctaca acatttttaa ttgcttcttt caatttcaat atacttccag
      301 ctaagttgcc agattctaga cgtgctgttc cttcttttac tactactgga aattcgccta
      361 aattgtaatt tccatcaggc atcccaccgg ccatcataca gtctgtaatt aatgcgacat
      421 gatcatgtcc tgctttttcc attaggattc ccgcagcatt tggatgtaca tgatggccat
      481 cacaaatcaa ttcagaaaag acatgttcta aagataataa cgcacccacc atacctggtt
      541 cgcgatgatt caatccacgc ataccattga aagcatggac aaatacactt gcgcctgctt
      601 caacagcttt ttgcgcttct tctagtgtag cattactgtg tcctagagaa acaactactc
      661 cttcttcagt cacttgtttg acaaactctt ctacaccttg acgttccggt gctaaagcaa
      721 tttttttgat taaaccacct gaagcttctt gccattcatg gaaagtatcc aaatcaggat
      781 ccccgaaata acttgggttt tgtgcccctt tgtgttcttc tgtgaagaaa ggaccttcaa
      841 aatagattcc tttgatttta gctcctggtg cttcctgata cattttgccg attgtctcag
      901 ctacatcttt taaacgttct ttgcttgacg tcaatgttgt tggtaaaaat gaagtcactc
      961 cacaagataa cagaccttca gacatcactt tgatgccttc tgcatcatta tccattacat
     1021 catgattcat atagccgtga atatgtgtat ctaccaaacc aggagcaatc catttgccgc
     1081 tttggtcaat gatgttgact tctccctcag gtagttcttt ggtgtattca ccaaaaagtc
     1141 catcggtaat ttcaagatag cctgcatgtt tgacacctga cgctaaaaag aatttgtcag
     1201 catagataaa agttctcatt ctattctctc cctttgtttt tctacctcta aagtactctt
     1261 atttagagaa gaagtcaaga atggtctaca ccatttacaa attttttgaa atttgtttca
     1321 cagcttgtgc taagccttca tagaatgctg ctctttctgg ttcttcttta cgcttgtata
     1381 ttaagctttt aagcacaaat aaatcattga ggtaatagta gctattcttc tcttgagccc
     1441 aatagattcc ttgattcacc tgttctaacg cttcgtcgat ttcattttga tggaataaga
     1501 aatttccata attataaaaa atttgcgaaa attcactttt tgatcgttca tcagctgatt
     1561 gatgcagcag ttggatgctt cgactcaaat agtagcgtgc ttcagccgtt tttcctgcca
     1621 caccatatat ttttccgata caactaatca gttgtatttc tgtgtcagaa acatggattc
     1681 tttctttttg gctagagtag cttaatgctt ctttcaaata ataaagggac tgagagatat
     1741 tttgacgcaa cgtgaattcg caaacaccga gataataata atagcgttga aagtcagttg
     1801 cttgatacag attttcagaa atatcagaag attttagtac ttgttctaac ttccgataat
     1861 cacgtttttg gaaacaacta tccaacagtt caaataattt atcgtttgtc cgagtagttt
     1921 ttcgagagag attcatcaca tgatcaatcg ttacccctag ccgttgacaa atctgcatca
     1981 tcaccaatac attcggcagt tcctcattat tttcgatccg acttaataca ctttgtgaac
     2041 aaatatcatg cgaaagcatt ttttgcgtca ttttttgttc ttttcgaatc tctttaatga
     2101 tcgttccaaa ggactcgatt aacgtttcca taagtatcct ccccaaaata tgcgtattcg
     2161 cattctattc gtattttact ttaggaagtc gcaaacttca agaaaagact tgcaactaaa
     2221 taatatactt tagttattta agaaaactcc actagaaaga gagcctcagt gtgtttgtct
     2281 gtggccctcc tactgaaaaa tttggttttt aaaatcattt ttggcgatag gttgctagga
     2341 attctctcat tttcttagct gtttgtttca aatcatccat tttaggaaga taaacgattc
     2401 gaaaatgatc tggctggttc caattaaatc caccaccatg tacaagtaga atatgatgtt
     2461 catgtaaaaa gtccaaaacg aatttttcat catccaaaat attgaatcgc tttgtatcaa
     2521 tttttgggaa tatataaaaa gcagcttttg gttttacggc tgaaagacca ggaatatcat
     2581 tgattgcatt atagatatat tctctttgtt catagatcct tccgcctggc aggagcagct
     2641 catcgacact ttgataaccg cctagcgcgg tttggataat ttgctgagac aatacattgg
     2701 aacacaaacg catggaggac aacatattca gcccttctat atagcctttg acgtgtgact
     2761 tatctccact taggaccatc cagcctactc gaaatcctgc tacacgatga gatttggata
     2821 gaccgttcag cgtaacaacg aacaaatcag gcgctaatgt agcaatcgga atatgttcta
     2881 gaccatccat cactaagcga tcatagattt cgtcggaata aataatcagg ttattttgtc
     2941 gagctacttc aacgatttct tctaataatt cttttggata aagtgcccca gtaggattat
     3001 taggattaat gataacaata gcttttgtcc gactcgttat ttttgatttg atatcatcaa
     3061 tatcaggata ccattctgcc tcttcatcgc aaatataatg aacaggtgtg cctccagcta
     3121 atgcaataga agccgtccac aacggatagt ccggcattgg aaccagtact tcatctccat
     3181 tgtccagcaa cccctgcata cacatcgtga tcaattcgct cactccgttg ccggtgtaga
     3241 tatcattgat cgtcacattc ggaaaatgtt tcagttggca atattgctca attgcttttc
     3301 tagcagaaaa gatccccttg gaatctgagt agccttctga gtcacgggca ttcatgatca
     3361 aatcacgaat gacttcgttt ggtgcttcaa atccaaaagg tgcgggattt cccgtgttca
     3421 attttaaaat actaatgccc tcttcttgca tccgctccgc ttcttctaga acgggaccgc
     3481 gaacatcgta actgacacct tctaatttgt tggatttttc aaattctctc atcacttagc
     3541 caatccctca tttcaaaaag ttagaacttt ccaaattttt tgttatttac tatttcaata
     3601 tcatactcca tcctagaaaa ttttgccact tatttaggaa aaacgtaagt attaaaaaaa
     3661 cacaaacaag cgttgagaac ttaattgtca tgatagtcat tccatagatt cgctgcccaa
     3721 tccattgcaa ttccacaaat gattcctagc ataaaaccaa gcaaaaaacg tccgaatgca
     3781 agtccaatca atacacctaa gatcattccc agtcctgaaa attctccgtc atatttctcc
     3841 atcttaataa gtcctcccat ttttgagctt gacaactatg taaaacctcg ctatcattag
     3901 tgtgttatta ttacgcagat gtgttggaat tggcagacaa gcatgattga gggtcatgtg
     3961 agcgttagct cgtgtgggtt cgagtcccat catctgcact acaactcgtg aagttaattt
     4021 tgcttcacga gtttttttgt ttgaacgttt ctttatgcca ttatatacat atcactgtat
     4081 cctttacaaa aagaacgtac aatcatgtaa acagcctgag gtgagtatat gaaggtcgga
     4141 aacaagctac tcgtagaaac ttgcaagtgt taaaacaatt aatcgaaaaa cgagtaaaat
     4201 ccctcaccta atactaaagc gagggatttt ttgtatatta tctgtttatc accgttatta
     4261 taaaggtaca aataccaagc acggttaatc caaattgcat aaattcattt ttttgtacac
     4321 tcatgctttt cattagctct ttaactgcca ttagtactaa gatggcgata acaacataaa
     4381 gtatatagta cgtccgcatt ccggaagccc atactgtggg agaaaatccc ataattaacc
     4441 ttgaacaaaa tcccattatc atcaaaataa ctaaaaaaat agaagttttc ttattgtcaa
     4501 aacttaaata aattccaaca attaaacata gtaataatgc caagactact aaagtagcta
     4561 tccaagtacc tggataaaaa agtgacagtt tcgttcctaa ctctgtaaag agattattca
     4621 ggttactact actccaaatc attgcccttt tatttccatt gacgtaagta aagctttgac
     4681 ccattgtatt accaaaataa ataatcaggt ttaagaaaaa cggtaatgca gtcaatatac
     4741 gcacataata gttttgacat ttcctatatg ttagaaggaa aattaagaaa aatagcaata
     4801 aaaataagat attcatgtct aaaaaaacgg ctttccaaat gatgaaatac ctaaatctaa
     4861 cttattaaga aagttgaaat ttttatattc aggaaaccat ttattaattt catgtgcagc
     4921 acgtaatgca ttccctggag ttgtcaaaga gaaaattaat tctgctaagc ttattattga
     4981 aaatactgat aacttataat tatatcgtcc attgacaatc aaatataaac taactatact
     5041 tgttagaaca aaaaaacagg cgttgacttg ttcttgatta gttaaaaata tcaataaagg
     5101 aatacttacc caatatattt ttctatttat ttcttctcct cttaagagtt gaaaaaacgg
     5161 ataaaaagtt agtaaactaa atgctactgg ccactgatag ttcaaggtgg ttgctctcca
     5221 accggtttcc cccattactg tcaatggtat taaacagtat atagagcaaa ccaataataa
     5281 atttttaaca tttattttct caaaaacat
//
LOCUS       DBNICI010000167         5206 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.171, whole genome shotgun sequence.
ACCESSION   DBNICI010000167 DBNICI010000000
VERSION     DBNICI010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.171"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..1367
                     /locus_tag="AC1ZCC_002476"
     CDS             <1..1367
                     /locus_tag="AC1ZCC_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630695.1"
                     /translation="FYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALT
                     IIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQ
                     IKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSI
                     IQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLF
                     QKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAA
                     KENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGES
                     WEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDV
                     NAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKK
                     NENK"
     gene            1354..2988
                     /locus_tag="AC1ZCC_002477"
     CDS             1354..2988
                     /locus_tag="AC1ZCC_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HIQ1630696.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            3163..3996
                     /gene="msrA"
                     /locus_tag="AC1ZCC_002478"
     CDS             3163..3996
                     /gene="msrA"
                     /locus_tag="AC1ZCC_002478"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIQ1630697.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            4109..5065
                     /locus_tag="AC1ZCC_002479"
     CDS             4109..5065
                     /locus_tag="AC1ZCC_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIQ1630698.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT         1824 a          806 c          951 g         1625 t
ORIGIN      
        1 gtttttatta tggagaaaag ttttttaaaa agatagatgc tgctttttta tttagttgtt
       61 tatatacttt tgcaacttat cgaacagttg atatctatta tcgatccgca attgcagaag
      121 caatcgctct tacgattatc ccagctgttt tatattatgc ttatggattg atattcaata
      181 gagaaaaaaa agtcatcgct ttatctgtca gtataagtct acttgtgtat actcatgtct
      241 taactacact attgtgtagt gcattgattg cttgtatgat tatagttcgt ttgtttttaa
      301 gcaagcaaat aaaaaacgat ataaaatttg tggtcgtgaa tttagtaaag gctgtttttt
      361 tgacttgtgc attaactagc tatttttggt atcctatgtt gcagcagatg aattatcaag
      421 aaatacacag tcccttcaaa acagatttgc agctagaagc actttctatt tcagatagta
      481 tcattcaagc gttaaataac gatattacaa catacactat ggggttatta ggattggttg
      541 cgttgatttt tcctttattc atactgaaag aaatggagaa aaaagaaaaa atcatctatg
      601 ttggcgtagt ttctacatgg gtatgcgtaa ctaatttatt tccatggttt ttgtttcaaa
      661 aaacaccgat aagcttgctc cagtttcctt ggcgtatctt aggtatccaa atcttttttt
      721 cttcaatgat cataacgctt gtttttatga aaaagactat gaataaaaaa tattcatggt
      781 taatcattgg cgctactgtg gtgtttctac tcgtcacgtc tatagcagca aaagaaaatt
      841 acgcaaaagt tatccaatca aaacacggtc atattgttat caatgaagaa acactcccac
      901 gttatacaac atcagatatg ggaggattat acgattacgc cccaacagaa gcgataaaag
      961 aaaggcctca cttagaaaaa catgaagtaa aagtaggaga atcgtgggaa aaagggaagt
     1021 ataaagctgc agataataac attacttaca cagtggcaag caacaaaaag caaaaagtga
     1081 cagtaccagt atatgcttat ttaggtacac aggtaaaagt aaacggtaaa gtcgtaaatg
     1141 actcttataa aaaacatggc ttagtgaatg tggatgtaaa tgctggagaa aataagatag
     1201 aaattacaac aaaatatact ccaactgctc tcatggcttt atggatcagt attctgactt
     1261 attgtttagt ggtgtattca agttttaaac gatttgcacc taataagttg aaagattctc
     1321 gatatgtgac taaaaaggag ataaacaaga agaatgaaaa taaataaaaa agtagtaata
     1381 ttgagtggat taattatagt tagtagtata tattctttca tctttttcaa aaatacacag
     1441 agtatttata ccactggaga tctttctttt cacttatctc gaatcaaggg gttatcgact
     1501 atttttacca gtccgattaa ttatgagaca ttcaattata ctggatatgg tgtaaattat
     1561 ttttatccct ttctgacttt ttttccagct gtaatgttat attggatgac aaaaaatttg
     1621 attgtaagct atattattta cgtttggtta ttgaacttgt gtacaacact cgtagcttat
     1681 cattatggcg agcggttttt aaaacaaaaa aaagcggcat ttttatttag ttgtctctat
     1741 attttctctg cttatcggac agtagacatc tactatcgtt ccgcaattgc cgaagctatc
     1801 gcaataacac ttgttatccc tgtattattt tatgcttatc aaatcatctc tggaaaagaa
     1861 gaaaagtatc cgtctgtaaa gctagcattg agtatgagtt tgctagtgta cagccatgta
     1921 ctatcaacat taatgagtac agcattaatt atcattttta tttttattcg tttggtttct
     1981 aaaggtttca agaatgcaga ttttattgct atatttaaaa aattatttag tgcggcagga
     2041 atgacattgg ttctgacgag tgctttttgg tatccgatgt ttgaacaaat gctctaccaa
     2101 aaaatcaaca aacctagcgt gacaaattta tatgctcatg catctaatgt gttcgatagt
     2161 ctgactgaag ctatgaacaa cgacttaact acttactcaa tgggactagt tggtctgtta
     2221 tctttatgta ttcccctgat tttatttaaa aaattaacaa acatagaaaa aaagatttat
     2281 tatggtacgt gtttgacttg gctagctaca actagtttgg tgccatggta tcttctgcaa
     2341 aatacgccag ccaaattgtt acaatttcct tggcggatct taagcttgca aataatattc
     2401 tcttctctga tattaacgat gatatttttc aaaaatagaa gatacaataa gacaagagaa
     2461 ctattttatt tatttatgac tgttttgtta ttaatcactt taactgtttc tagtaaaaaa
     2521 aatttcaatc aaaaaataat ttctcagccg ggacatattg ttgtaaacaa agaaacagta
     2581 gaggcgtata ccactaaagg aatggattat tttttatatg attattctcc tatcgcttca
     2641 ttggagaata aggagcatat aataaaacac gaagcaaaga ttggcagtca atgggtaaac
     2701 cttccttata aaacaactgg tagttctatc atatatagag taactaatga gaagagccaa
     2761 gtaatcacac ttcctgtata tgcttattta ggaacgaatg tagaaataaa tcatcaacct
     2821 gtaaaggagc aggtgaatgc taatggattg gtacaaataa gcggagtcaa aggagttgct
     2881 gatattaaag taagcacaag ttatacagta acgactatta tttcttatct tgttacatta
     2941 actgcatatt tatgtttatt ttttaaaata gttttccata aaaggtaaat taacttttca
     3001 tttataaaat aaattgatta aaaaggagaa tctgtcgata aggattctct cttttttata
     3061 cgaaaaatct gtagggggat gaagtaatcg cttatttcct cactttatga taaattacag
     3121 ctaaagtcac tagcttgtct tcttcggaaa ggaggagaat ctatggatga ggaacaaata
     3181 ttcattcaaa cactttataa tctgattttg aatccgaata cacgagattg ggaacgaaaa
     3241 gtattgattc agacaaagaa tgacataaga gaaaatatat ccgttaaaga acagttatca
     3301 aaactagaag caacactacg tccattagct atccgaatga atctgacgcc tgatgtaatg
     3361 gacttttatt tattgattac ggaaggattt gataaggagc aaaaatatga cttttccaag
     3421 catgccatgc aagatgctga ctatcaagaa cgtgcagtat ttgcaggggg atgtttctgg
     3481 tgtatggtcg agccgtttga atcgaaaaaa gggatcctat ctgttttgtc aggatacacg
     3541 ggcggacatg ttgaaaaacc aaattatgat caagtaagtg gtggttacac aggtcatgtg
     3601 gaagctgtgg agatcattta tgacacaaga gaaataagtt atagcgagtt gctgacaatc
     3661 tattggcaaa ttactgatcc cacagataca tttggacaat ttcaagatag agggaagcaa
     3721 tatcgtccag tgatttttta tcaaggcgaa cgacaaaaag aattagcgga acaatcaaaa
     3781 cagaaactag acagttcagg aactttccac caaccaatcg tcacaaaaat cgaaccagcc
     3841 ggtacgtttt ggccagcaga aaactatcat cagcaatttt acaaaaaaca accgaaacgt
     3901 tataaaaaaa tccagcaagc acgtaatcaa tttctgatct atcaacgagt aaaaaacaaa
     3961 tggcaaaaaa atatacgaaa aaatcatttt gattaactcc ttttctttaa aatgtagtaa
     4021 actaaaaata gaaaaaagaa agcgttttaa aaagatttga tgaggaggag aaaaattgta
     4081 tattgcagat agtgcacgtt atgaaaaaat ggaatatcgt aaatccggtc attcaggttt
     4141 gaaattaccg attttatctc tcggattatg gcaaaatttt ggtgattatg atcctatcca
     4201 taatcaaaga gagattttaa gaggcgcatt cgacatggga attacccatt tcgaccttgc
     4261 gaataattat ggaggccctg ctggagctgc agagaaaaac tttgggcgta tttttagaga
     4321 agattttcaa gcctaccgtg atgaactgat tatttctagc aaagcaggtt atcatatgtg
     4381 ggaagggcca tatggggaat ggggctctcg taaatccatc atttcaagtt gtgaccaaag
     4441 tctccaaaga atggggctag attatgttga catcttctat catcatcgcc cagaccctga
     4501 tacacctttg gaagaaacag cagaagcttt gatgcaactt gtaagacaag gaaaagcatt
     4561 atatattggt atatccaact ataatggaga agatacaaaa aaaatgactg aaattttaaa
     4621 aagaaaggaa gcaccgttca ttattcatca gatgcgctac aatatgtttt ctcgtgcact
     4681 actagaagac gatctatctc cagtattaga agaagaaggg ctaggggcaa tcacgttcag
     4741 tccgcttgct caagggttgc tgacaaatcg ttatctacat ggtattcctg aagattctag
     4801 agcacatcgt aaggaaatac cattcctttc agaagaacaa gttggatcaa ctctggaaaa
     4861 aatcaaagca ctgcaaacaa tcgccgtatc acgaggacaa tcattagcgc aaatggccct
     4921 tgcatggaat ttgcgacaaa aatctgttac aagtgtactg gtcggagcaa gtcggttaag
     4981 tcagttacaa gaaagtgttc gaatgatgga taatttagat ttctcaccag aagaagaatt
     5041 gagaatcgac caaattctag aatagaaacg atgtaatcaa actttttatc catatattaa
     5101 aaaaaacaaa agatgacatc aagaatcgaa tgaagatcgt ctgttattga acatcatatt
     5161 ttttgtcgtt caaaataaaa atgatcgaag gctaggagtg aatttt
//
LOCUS       DBNICI010000168         5045 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.172, whole genome shotgun sequence.
ACCESSION   DBNICI010000168 DBNICI010000000
VERSION     DBNICI010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.172"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(63..1376)
                     /locus_tag="AC1ZCC_002480"
     CDS             complement(63..1376)
                     /locus_tag="AC1ZCC_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HIQ1630699.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(1621..1845)
                     /locus_tag="AC1ZCC_002481"
     CDS             complement(1621..1845)
                     /locus_tag="AC1ZCC_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630700.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(1865..2287)
                     /locus_tag="AC1ZCC_002482"
     CDS             complement(1865..2287)
                     /locus_tag="AC1ZCC_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIQ1630701.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(2429..3139)
                     /locus_tag="AC1ZCC_002483"
     CDS             complement(2429..3139)
                     /locus_tag="AC1ZCC_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B3/B4 domain-containing protein"
                     /protein_id="HIQ1630702.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(3157..3654)
                     /locus_tag="AC1ZCC_002484"
     CDS             complement(3157..3654)
                     /locus_tag="AC1ZCC_002484"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1630703.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(3691..4143)
                     /locus_tag="AC1ZCC_002485"
     CDS             complement(3691..4143)
                     /locus_tag="AC1ZCC_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIQ1630704.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(4278..4883)
                     /locus_tag="AC1ZCC_002486"
     CDS             complement(4278..4883)
                     /locus_tag="AC1ZCC_002486"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIQ1630705.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
BASE COUNT         1455 a         1051 c          814 g         1725 t
ORIGIN      
        1 agaaactatt ttttcgtttt tttctctaat actcaggcca gaacgctgat ttcacaacct
       61 ctttatttca cattcaatat ccacatgaat cccacaaata caaagaacag accatacatg
      121 attggagata cttcttttcc tcttttagct gctaacatgg taattggata gaagataaaa
      181 cctaatgcaa tcccatctga aatactataa gtcaaaggca tgccgatcaa gatcaagaat
      241 gaagggatcg caatctctaa gtttccccat tcgatttgtc gcaaagattg tgccatcaat
      301 actcctacca taatcaatgc tggtgcagtt acttgtgaag taacaactga tagtaatgga
      361 gaaaagaaca agcctaacaa gaagaaaatc cctgtagtaa tagctgtgat acctgaacgt
      421 cctccaacgg cgataccagc tgatgattct acaaatgcac caacaggaga agtaccaaat
      481 aaggaacccg caagcatagc tgttgaatca gaagctaaag ctttccctac acgaggcatc
      541 ttgttgtctt tcatgaaacc tgcttggttt gctaatccaa ctaaagttcc tgctgtatca
      601 aagaaagtca caagcaagaa tgtcagcact acgacccaca tttgaagaga attgatgtcc
      661 cctacatgtt ttaatgcaac taaaaatgtt ggtttcaaac ttggtgctgc ggcaacaatt
      721 tcagaaggca ttggaatcaa acctgtgacc agacctaaaa tcgttgtagc tgccatcccg
      781 atgaagatgc cacctggtac acgccgaaca agcaagatag cagttacgac taatccaaaa
      841 atcgtcaacc aagtagaacc aacgttgagt gatcctaatc ctactaatgt agattcattc
      901 gaaacgatga tcccaccttc gctcagacca aggaaagcga taaatagacc gataccgcca
      961 gaaatcgcat atttcaaatc tgctgggatc gcatcaatga tcatttcccg cagcttgaag
     1021 attgtgatca aaataaaaat caaagaagca acgaatacgc cggctaatgc tgtttcccat
     1081 ggaatcccca ttcctaaaca aacagaataa gcaaaaaaag cattgatccc aagagcaggt
     1141 gccgttccaa ttggataacg ggctaatacc cccattaaaa cacagccgat cgcactggct
     1201 aatgcagtcg cagtaaacac tgctccttca tccattccag aagcacctaa tacagttgga
     1261 tttacaaata agatataagc cattgagata aatgttgtaa atcctgccag tacctcacgt
     1321 tttaccgtgg tgttcaattt ttctatttcg aagtaggaaa gtattttttc cttcatcttc
     1381 catcccccta aatccgaaca ttataaataa agtccgttaa aatttcgatt tattaataaa
     1441 acaacgtgtt ttatcatata ctaaaacaaa acataagtaa acaaaaaatt cggattttaa
     1501 gcaaaatccg aacgttccat ctgattaaat ccctttatct caaagttcat gaacattcac
     1561 tttggattac aagaatttct ttattgtgtt aataaagttt cttcattcta gtctcaaaaa
     1621 tcaatagtgt gatttgattt taccatttcg tttctgttct tctttgatga attcttttgt
     1681 atcgacagga ttcagattat ttagtccgcc aaaacctgta tcgaccattt ttccccagcc
     1741 ttttgtcttc ttttttcctg atttgagatt ctttgctcga agcttctcac tatgttttgc
     1801 tcgccgatac ttcaaataac cttctaatct tcccattaga tccactgttt ttcctccttt
     1861 ttattcaatc agacgactca gcttttccta taacaaaata attgccttca gggtcagaaa
     1921 aattgaaagt caagatgcca ttgttatctg tgatttctcc tggttcttcc atgttctcgt
     1981 agatagtttc aaattcatcc gtaaaaaata ctaaagaagg cggtggtcct aatacctctg
     2041 gtgaaaactt ctgcacaaat acttttggga atacgcctaa ttctaattct tttgagatgc
     2101 tgagtacaat actcttaaat tcttctggca attcgattgt ttcagaaatt tctgcaccaa
     2161 ttttttctac aaagaatcgg caaatcatct ctacgtcatt gacataaagc atcacacgtg
     2221 ttttttgtat catcatttga cccttctttc tatttgattt tctttcctgt ctataaactg
     2281 attccattat atcacgaaag cgcaaacacc ttgtgtttga tactattcaa gaaaaataaa
     2341 gcaatagttt ctaggattgc tttcctccac aaactttttt tacaaaaaaa aaacggcaat
     2401 gaatgccgct cttcttattt cgattctttt ataattctgc ttctgattca tctttggtta
     2461 gtgtgtgtct aacactagtt acgccaaatg tttgatcgat tttttccttc aacgcttcga
     2521 ttgcctgtgc gccacgtgcc gcttgttcag cattgatgga ttcaatgacc agcaccgctg
     2581 cttgtgtttc ttccgtcagc atcgttcttt gggcttcacg ccagttgaga cagcgacaga
     2641 tcgctccttg ttcatcatag tagatgattt cttccggaag tgccggagca tcggaatctg
     2701 cacctaaagg caggaatgat tcgccacctt gggcttttcc taaatgcaga tttcctgcga
     2761 tcttttcgat atcttctccg ccgcaaggta cgccaaaaga caaggaaata ctattataga
     2821 tatctacaag cggattgatt gggaaaaatt cccgttcttg atttgcacgt ttcagtaaag
     2881 cttcaataga agaacgtgca ccttttttag tcttgaatgt ggtaaatgcc tgacgccact
     2941 gagcaatgac ttcgttttgg ctgaatgttt cttctgtaag gtaatttctt gcttcttttg
     3001 ctgcctcatt caataattga tgatacgttt cttcactttc ttttgttacg tggttatcga
     3061 taccatttac ggataagatt tcgatggttg cttcaggaaa tacttcccag aaattggtat
     3121 ccactattac ttttttcatc tgttcacacc tcttgattag aataattgct tgatgaatag
     3181 atagtctgtt tggggatcat ctcccatata aaaaacatgt tcgctgtatt tgataaatcc
     3241 ttttttacga taaaaagcga gcgcaacatc gttgtgttcc caaacaccga gccaaatggc
     3301 tgttttttgc ttttcttttg ctttttcaat agctgtatct agtaaaaatg taccgatacc
     3361 ttgatgttta aatttttttc gtacatagat tcgctcgatt tctaaaaaat gatcagccat
     3421 ttcctcagat tgactcgttc ctgtatttag tttcaggtaa cccgcaggct cttgatcgat
     3481 actcactagg aaaaaatcgg tttctttatc ttttagctct tctgtaagtt tttctagtgc
     3541 ataagcttgt gtcaagtaat cagctaaatg ctccggtgaa ttatcttttc caaatgtatc
     3601 tgtaaatgtt tcgatactaa tttgttgtag taagtctaca tctgaaggcg tcatttttcg
     3661 aatttcgtat ttattcacgg cttcttcctt tcaatagatc cttttattgc cttttttgac
     3721 aaaattccaa tcttcgctaa cattcttgca tactttattt aaaagatatt ccaattgttt
     3781 tgcttcttcg tcagaaagac catttaatgc gactgcattg gagtagttgt tttctcgaat
     3841 gatgaaagga tagatttctg ctcctttttt agtcggatag agtttcttga tttttttatt
     3901 ttctttatct tccaaacgtt cgatcatccc attggattct aatttcttga ttgctcgggc
     3961 agtcgttgta cgatctactt tgatcatttc tgctagtttt tcttggatga ttccaggatt
     4021 ttcgcaaata cgtactaaat aaagatactg tcctcttgtt aaatcgactt ctttgaattc
     4081 gatattgcta atagaatcta gcgctcgggc aatcattccg atgtctcgaa ggatttcagc
     4141 catttcatcc cacctttatt ttgtttgtaa gactagttca cctctttttg ttgcaaatgc
     4201 aactattttc aataaaaaaa tcaagttctt ttttaataac tagtaaagta tattcttttc
     4261 ttcttttacg aaaaagctca atcacaatag atctggtttt tgttttctaa aataagtaac
     4321 tgtttcttcg ttgttcgctt ttctgatagc tttggcaact cttctatact aaaaaatccc
     4381 atgttggaag tttcattatt ctcaataaac ttggcctctt caccgtggat cgcacaagca
     4441 aaaacaagtt tataatactg aaaaagctgt ggaatatctt tttgtttatt cgtatcaaaa
     4501 actgctcgca actgagtagt ttctactgtt aatcctgttt cttcatagac ttctttacgc
     4561 acattttctt caggagacaa tcctatttca gcaaatccac caggaagtga ccattctttc
     4621 gtccgtttat cttcgattag taggaccttg ccatcttttt tgatataggc acgaacatct
     4681 acttttggtg ttggatgtcc ttctccaatc gttgtgagcg cttttatttc ttcgaaagtt
     4741 tcttttccta tttcactgat cagttccaat gaaatcgtcc ggatctcctc aaaacgctct
     4801 ttatcaaata catcttttcc ataatataga ccagcttgag aaagtgcgag tagtcttttg
     4861 tattgagcca acttcttgtt cattctcttc ccttcttttt tagcaaatgt atgactatgt
     4921 aatcaagtat agaagactgg tttaattacg gcaaataaat ataatgaaat ctgcaataaa
     4981 taaaagaatg ttaaaaaact cgagaacaaa agacattcga cttctctggg gctgtgaata
     5041 aatgg
//
LOCUS       DBNICI010000169         5045 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.173, whole genome shotgun sequence.
ACCESSION   DBNICI010000169 DBNICI010000000
VERSION     DBNICI010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.173"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(41..1180)
                     /locus_tag="AC1ZCC_002487"
     CDS             complement(41..1180)
                     /locus_tag="AC1ZCC_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HIQ1630706.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1579..2877)
                     /gene="eno"
                     /locus_tag="AC1ZCC_002488"
     CDS             complement(1579..2877)
                     /gene="eno"
                     /locus_tag="AC1ZCC_002488"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIQ1630707.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2993..3748)
                     /gene="tpiA"
                     /locus_tag="AC1ZCC_002489"
     CDS             complement(2993..3748)
                     /gene="tpiA"
                     /locus_tag="AC1ZCC_002489"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIQ1630708.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3852..5042)
                     /locus_tag="AC1ZCC_002490"
     CDS             complement(3852..5042)
                     /locus_tag="AC1ZCC_002490"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIQ1630709.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
BASE COUNT         1283 a         1068 c          943 g         1751 t
ORIGIN      
        1 atgctaatct ggctctgttt tttcatttat taaaaaaatc ttacgctaag taggataaaa
       61 taattccttg atatttttcg atcatatcca aataattatc gatttcaaca tactcatcta
      121 cttgatgagg caaagtaacg acaccaggcc caaagacgac aaagtcgaag gaatggttag
      181 ctttcgtaaa ttcggctgca tccgttgttg ctgccgctcc aactaaaggt aatggctggc
      241 tgaattgttg ctgaatgcaa tgaatcaaag gagaatctgg atcagctttg acaggtattt
      301 tgttgtaatc gatcatcaat tccaaatgat agtctgtctc ttgattcaat tcattgacga
      361 ttgattgcag caatgcaatg attttatcat ttgggtattc tggaatcgat cgaatgttcc
      421 cttggagctg cgcatggctt gggatgctgt tgacttgatt cccgccactg atcaatgtaa
      481 cattatggat tgtttttcct aggacaggat tctcgattgt ttcagcgagg tgattcattt
      541 cagcatttgc tttcgtaatg aattcattta ggtgattgat tgcattataa ccttggtctg
      601 gcatagagct atgcgcttct tttccgtgcg aggtgactgt atagttgatt gatcccatgt
      661 gcgtatacat caaactgtaa ttggttggct ctccgatgat caaggcatct aagtcatcta
      721 catagcctgc tttcgtgagc tgttccccac ctagttcacc cacttcttct ccgacagtgg
      781 ctaataattt gactgttccg ttaaatggtt ttcctgactc tttcagttcg atcatggcaa
      841 tcaccattgc tgccaagcca ctcttcatat ctgtagcacc acgtccgtac aaacggttcc
      901 catggatttc tgctgcaaag ggtgcatagg tccaagacga ttcatcacca gccgccacga
      961 catccatatg cccagatagg cctaacactt ttcctgattg gcccttttgg taacgagcaa
     1021 tcaaattatc tctaccatct ctgtaagaaa caatctctcc tgttatgtca tgctttgcca
     1081 aaagcttatt caaataggct gctacttcac cttcgtttcc attcactgat ttaattcgga
     1141 tgatctcttg taaaatcgcg attttttctt cttttttcat ctaatcctct cccttgccat
     1201 ttctttttat tgtatttgag tgtgctcgaa ttttcaaata ggagcaacca aagtaaaaat
     1261 gaagaaagag agccacttta tgaaaaatat gctgctactt ccctcctatt ttatttatac
     1321 gagaggactt gctcatagat tcctattcta ctgttctatc cttaaaagct cttatatgat
     1381 gctagttatt gttagctttc ctttttcaag gatgatcttt tcttgtacca ttctttgtaa
     1441 acatcatata cagaagctag ttgcaaaaaa actctctacc tgctgatatt tcagcaagta
     1501 gagagttcga ttacttaact aagtgatcat actcgactat ctttcaatag tcgatcatgt
     1561 atcttcatta gaaaagaatt atttgttttt taagttgtag aaagatttca aacctttgta
     1621 ttctgcaact tcaccaagtt ggtcttcgat acgtagtaat tggttgtatt tagcaatacg
     1681 gtcagtacgt gatagagaac cagttttgat ttggccagcg tttgttgcaa cagcgatatc
     1741 agagattgtt gaatcttctg tttcaccaga acggtgagat acaactgctg tgtagccagc
     1801 ttctttagcc atttcgatag cttcgaaagt ttctgttaat gtaccgattt ggttcacttt
     1861 gataaggatt gagttagcaa ttcctttttc gataccttca gataattttt gagtgtttgt
     1921 tacgaacaag tcgtcaccaa ctaattgaac tttgtcgcct aatacgtcag ttaatttctt
     1981 gaatccgtcc cagtcgtttt cgtctaatcc gtcttcgatt gagatgattg gatatttaga
     2041 aactaattct tcgtagaatt tgatcatttc gtcagttgtt ttttcgcctt cgcctgaatc
     2101 agctagtacg taaacacctt tttctttgtc gtagaattct gaagaagcag catccatagc
     2161 aagaacaacg tctttaccag gtacatagcc agctttttcg attgcttcga tgattacttc
     2221 aaaaccttct tcgtttgaac caaggtttgg agcaaatcca ccttcgtcac caacagaagt
     2281 tgctaatccg cgagctttca agattgaagc taatgcgtgg aatacttcag cacccatacg
     2341 tagagcttct ttgaatgttg gagcgccaac aggcatgatc atgaattctt ggaagtcgat
     2401 tgagttatca gcatgtgatc cgccgttgat gatgttcatc attggtgttg gcaatacttt
     2461 tgtgttgaat ccgcctaggt agtggtataa aggtacttct aggtagtcag cagctgcacg
     2521 tgcaacagcg atagaaacac ctaaaatagc gttcgcacct aatttacctt tgttaggagt
     2581 tccgtctaaa gcgatcatag ctttgtcgat agccatttgg tcacgaacat cgtagccaat
     2641 gattgcttca gcaatgatgt tgtttacgtt gtcgacagct ttagttacac ctttaccgcc
     2701 ataacgagct ttgtctccgt cacgtaattc aactgcttcg tattcaccag ttgaagcacc
     2761 tgaaggaacc attccgcggc caaaagctcc gctttctgtg tatacttcta cttcgattgt
     2821 tgggttacca cgtgagtcta agatctcgcg agcgtaaaca tcagtaataa ttgacatgtt
     2881 ttgtctctcc tttgagtttg ttttttccta agggatttcc ctcatatacg attttagtta
     2941 attttgctca ttagtgcaaa catttcttgc atttttcgca aattttcaac aattatttta
     3001 cagcgtctaa caatgacaag aatgaatctg cttgcaagct tgcgccaccg actaaagcac
     3061 cgtcaacatt ttcttttgcc atgtattcag caatattttc tggttttaca gaaccgccgt
     3121 attggatacg aactgcttct gatacttctt ttccataaag tttttcaact gtttgacgta
     3181 caactccaca aatttcgtca gcgatttgtg cgtcagctga tttaccagta ccgattgccc
     3241 agattggttc ataagcaata accatgtttg acacctgttc agctgaaaga tcagctaaac
     3301 cagctgtaat ttgtccttcg atccattcag cagttttgcc tgcttcatag gtttctaaag
     3361 attcgccaca gcataggatt ggtgtcatac catttgcaaa gatagctttc gcttttttat
     3421 tgatgtcttc gtctgtttca tggaaatatt cacgacgttc tgaatgtccg atgatcacat
     3481 aatgaacacc tagatcagca agagctgctg gtgaagtttc accagtgaac gcacctgagt
     3541 tttcccagta gcagttttgt gctgagattt ttagttctgt accttcagct gatgtcatta
     3601 attcttgtaa gaataatgca ggagatccga tgactgaatc aactttgtca tttgaaggga
     3661 ttttgttttt tactgcttca gcaaattctt ttgcttctga tgcagttttg ttcattttcc
     3721 agttacctgc gataataggt ttacgcattg aggaacacac tccttaattt attgaaaaat
     3781 ggctagggag tcatgaaaac tcccttcacc ttttgttcga ttactcgtca gaattgagca
     3841 aacttggtcg tttatttatc gttgattgct gctagtcctg gtaattcttt accttcaagc
     3901 aattccaagc ttgcgccgcc acctgttgag atgtgtgtga atttgtcagc aaatcctaat
     3961 tgttctgctg ctgcggctga gtctccgcca ccgatgattg ttgttgcgcc ttctaagtta
     4021 gcgattgctt cacaaacacc gattgtacct ttagcaaagt tactcatttc aaatacaccc
     4081 ataggtccgt tccaaacaac tgtttttgca ccttgtaatg tatcagcaaa taattttact
     4141 gatgctgggc cgatgtctaa agccatcaaa ccatcaggaa tgtctccttc gattacttgt
     4201 gtttcaacat catttgaaaa ttctggtgca cagacattgt cgattggcaa gacaagtttg
     4261 tcgcctgctt tttcgattaa ttcttttgct agttccactt tatcagcttc tactagagag
     4321 ttaccgatct tgattccttt tgcttcgtag aatgtgtaag tcattccgcc accgatcaag
     4381 attttatctg ctttaggaat caagttttcg attacgccga ttttatcaga aacttttgcg
     4441 ccaccaagga ttgcaatcat tggtcgtttt ggttcttcta ctgcttcacc gatgaacttg
     4501 atttcttttt ccatcaagaa tccagcaact gttgggatac cagttgaagc gatacctacg
     4561 ttagaagcat gtgcacggtg agctgtaccg aaagcatcgt taacaaatac atcgcctaat
     4621 gaagcccagt atttacctag ttctgtatca tttttgcttt cttttttacc gtcaatatct
     4681 tcaaatcgtg tattttcgaa tacgacaacg tcgccgtcat tcatgttgcg gattgcttct
     4741 tctaattgtt ctccgcgagt ttcaggtacg aatgttactt ctttacctaa taattcgcct
     4801 agacgttttg caactggagc taaagatttt ccttctttat cttcttctgt ttttacacgt
     4861 ccaaggtgag agaaaaggat tgcttttccg ccttgttcta gaacgtagtt gattgttggc
     4921 aatgctgctt tgatacgagt gtcgtcagtg atcacgccgt ctttcaaagg aacgttgaag
     4981 tcaacacgga caagaacttt tttgtctttt aaatcgatat ctttcactgt tttcttagcc
     5041 atttt
//
LOCUS       DBNICI010000170         5024 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.174, whole genome shotgun sequence.
ACCESSION   DBNICI010000170 DBNICI010000000
VERSION     DBNICI010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.174"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            282..521
                     /locus_tag="AC1ZCC_002491"
     CDS             282..521
                     /locus_tag="AC1ZCC_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630710.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(883..1143)
                     /locus_tag="AC1ZCC_002492"
     CDS             complement(883..1143)
                     /locus_tag="AC1ZCC_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630711.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1328..1825)
                     /locus_tag="AC1ZCC_002493"
     CDS             complement(1328..1825)
                     /locus_tag="AC1ZCC_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIQ1630712.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1955..2665)
                     /gene="budA"
                     /locus_tag="AC1ZCC_002494"
     CDS             complement(1955..2665)
                     /gene="budA"
                     /locus_tag="AC1ZCC_002494"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HIQ1630713.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="AC1ZCC_002495"
     CDS             complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="AC1ZCC_002495"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HIQ1630714.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4547..>5024)
                     /locus_tag="AC1ZCC_002496"
     CDS             complement(4547..>5024)
                     /locus_tag="AC1ZCC_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HIQ1630715.1"
                     /translation="CIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLFAWIV
                     SVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVLRIAF
                     YYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTMDL"
BASE COUNT         1529 a         1042 c          771 g         1682 t
ORIGIN      
        1 tatcctccta gcaactattt aaatacccaa aaaaattacc tctagataat atcataccta
       61 cgttttcact tataaatttt ttcataaaaa aagcgtttag aggcataatc gttaatacag
      121 atggtaacca atgtatctgg tttgaactgg gatatcaccc tctccatata tgtattacta
      181 agactaatag atactataaa agacttgata gttttgcatt gattttgcct aatactgagc
      241 ataggtgtat gctaaacgat actataagga gtgtgtacat aatgaaaaga attctgttta
      301 tcagtttatc aaatttattg ttacttactg cttgtgggac ttctgcatca aagaacatac
      361 cttcagatac gcaaatttcg aatagtatcg attcaacaat ttctacaaat gagaaaaatt
      421 ctccggtaga aacttccgat ataaaagttt ctacctctgc aaaaaacagg gtaaagaaac
      481 tgtcagcacg tctcctttat ttcaaacaaa ctctgaatta gcttcttcat cttcttcagc
      541 aaataatgat gatagtttcg cgtttactcc taactaaatg caacctttac aaatgcttta
      601 cgataacaag cctgagctaa atgatcctga tatcagtttt gcattttttg aaatgattgg
      661 atcggatgat ctttttaaaa ctactagttt gtctatccga aaataggaca gttctggcag
      721 tataggtttt tatcgagttt ctccaccagg cctcgttaca gagacagatt ctctaggcga
      781 accttttgaa taaatcaaaa atcatgaaca atcactgcca actaaataaa gtgccttcaa
      841 aagcttggtc ttctcgcttt tgaaggcact ttatttaaat ctttatgaaa ctaaagtatg
      901 ttcatatttg ttgctatagt tatatagata ttctgttgtt tccaactcat aaacgtgagt
      961 atattttgcc actacttttc cgcatttttc taatatttca ttgcgatcga cgtcatgata
     1021 ttcgtctacg cagatcacaa cacaattttc taatttactg attacttctc caaccacttt
     1081 ttgtttcaag ccaataggtt ggcaaatata tttttttcct aaaatgatag tttcgttatt
     1141 catatttcat ccatccttaa tgtttttcca ttatttaaaa tgataatacc attaaaatat
     1201 gattatgaaa atcaataaca cttgtgattt tataaaaaaa taaaaaaacc gaaaaacaga
     1261 aatgcccact gtctttccgg ctctttttat tcttgatttc tcttcttttt tgtttcaata
     1321 gacgatttta gttattaata aatgaaaaag catttatatt aaaatttgct aataaaagat
     1381 ctacatattt ttctagaaat gcttgatcga tttcatcata ccctttcgtt actccgcaat
     1441 cgatatctaa aacaccgatt aaattacctt cttttaccat tggcactacg atttctgatt
     1501 gtgcggctga atcgcaggaa atgtaatttg catgggtttt gacatcatca acgatcaatg
     1561 tttcttgttt ttcagctgat tctccacaaa ctccttttcc catcttgata cgtgtgcaag
     1621 aaactcgccc ttggaacggt cctaaaatca attcattacc atcgaataaa taatatcctg
     1681 caaagacagt ctcaggcaaa gtctcattca acaatgcaga tgaatttgct aaatttgcta
     1741 accagttatc ttctgcttct aataatcctt gttgctgtaa taataaaatt tcgtatgcat
     1801 cttttttctg tttttctgtc cacatgataa aaaccgctcc tttttgatct atggagaaga
     1861 ttatagctca gtttttttgt aaacgctagg tttcctcctc aaaaaaacga gatacgtcag
     1921 gttaatcacc ttgaacgcat ctcgctgtct ttttctattc tgcttgttgg atttctgctg
     1981 ctaaatcagc ataatcaatc gtttcgtgaa caaaagtcgg atcatcaact ggaaaatgct
     2041 gtacaaggga cgagatgcgg ctaatctcaa cttttccttc agccacttca aaatccatta
     2101 catgtccgcc aaatgtatgt gtatcatcga taaaatgcaa gtgaaatccc gctgcagcaa
     2161 ctccttggaa taattcaggc gtaaagaacc cgactaatgt tccttggaca ttgctttgtt
     2221 ggaattctgg ttgagactct gaaatttttg ctagtcttct gtaaggtttt tcttgcttcg
     2281 gcatgatacg gatgtgcata tttttgaata atccagttat cttcactgcc aaaaataggt
     2341 tttccccggc ttctcttact aatatgtctt ttttcaattg ttctccatct gcagtttttg
     2401 agacagaaaa actagtatct ggcgtaaaat ctgtaattgc tgcatagggg gtaagttcac
     2461 ttccatctaa ttgggcaact gagccatctg atcttccttg ataagctacg ccatctaaaa
     2521 agatgacttc accatcaagc ccatgcaatg ttcccaatcc cagagttcct ttttctaata
     2581 tatgagcaat cgtttctgtt ccatccatca agccagccat caaagctcct aatgtcccgt
     2641 gctggtataa gattttttca gtcatgtcga cacttcctta atagaattga tctggtaaga
     2701 ttgtttctcc caatttctca ttatcacggt aatcaatcgg aatatcaata attacgggtc
     2761 cttctgtcgc aaatccttct tctaaagctt tttctaattc ctctacactt gtagctcgga
     2821 ttcctttagc gccaaaagct tctgcatatt tcacaaaatc aactggacca aagtctacac
     2881 ctgacgcccg ctgatatttc attttttctt ggaactctac catattgtag tgtccatcat
     2941 tccaaatcaa atgaacgata ttcaattttt tacgtaccgc cgtttctaaa tcctgggcag
     3001 agaataaaaa tcccccatcg ccggaaactg atacgatttg tgtatttgga cgcaccaatg
     3061 cagcagaaat cgcccatggc aatgcaacac caagcgtttg cataccatta ctgaataaca
     3121 aatgacgagg ttcataactt ctaaagtggc gtgccatcca aatataatga ctacctacat
     3181 ctacggtaac agtcatatca tctgttactt ttgactgaag cgtgttaata acttctaaag
     3241 gatgcaagat acctgcttct ccttttgatt gaacaatgtc ccgctccgtt aattctgctt
     3301 gaagagatgc taaatattct tttgcatctt ctgacacttg ttgaggctct aatgaacctg
     3361 tcaataaatc aagagtggct gagatatcac cgatcaattc tctttcaggc tgcataaaag
     3421 gatcgatttc agctggcgct tcatcgatca caatgatccg cgcatctttt tccgcattcc
     3481 agtttcttgc ttcatattcg attggatcat agccaatagc aataactaga tcacttcgtt
     3541 tcaaaagcat atctcctggt tggtttctaa ataaacccac acgaccaaag aaatgcgctt
     3601 ctaattcacg tgagatcact ccagctgctt ggaatgtctc tacaacaggc aatgctgtct
     3661 ttccaactaa ttgcctgatg gctaacgttt ctttttcact tgatcctcgc atacctacta
     3721 atagtaccgg tagttttgct tctctaatgc gttcagctag gtaacggata tcatctgcag
     3781 aagcagaacc taatttagga tcgctcaatg gcttgatgac attcccttgg acaggtgcat
     3841 ccactacatc ttgcgggata ctgataaagc tcgcgccttt ttttgaactc ttagccatac
     3901 gataagcatt ggcaatcact tctgaaattg tttctggatc ttggatttct gcactgtatt
     3961 ttgtgattgg ctggaacagt gctgcattat ccatgctttg atgggtcaac ttcaatagat
     4021 cgcttcgttt tacttgtccg gcaattgcta aaactgggtc accttctgct gtcgctgtta
     4081 ccaaacctgt agctaagtta ctagcaccag gaccacttgt tgcgataact actcctggct
     4141 ctcctgtaat acgtccgatt gcttgtgcca taaatgcagc attttgttcg tgacgagtca
     4201 cgattaattc aggaccttga tcctctaatt cattaaatac accatcgatt tttgcacctg
     4261 gtataccgaa tacataatca acatgatgat taatcaaact ttctactaca gatttcgaac
     4321 cttgtttcat tttttcgtcc attgaacatc gtccttttta tgctttattt ttaattatta
     4381 gttagaaata atcacaaata attgatacag tttctaaacc tgtttcattc aaaagtatag
     4441 cacgttttat ttcatttgtt tagccatatg tttcaaaaaa tgaaaaagaa tgacagtttt
     4501 tacataaaaa ctgtcattct taattgaaag tttcaattta taaagtctac agatccatag
     4561 ttctcatcaa tttacgattg ttgaagtaaa tcaaaccaaa tacgatagaa ggacctagta
     4621 gaatacacca ttggggcttt acgaaaaaga agagaaacaa acttgtcaat agtaaaaaat
     4681 ctagtaaact cacataccat tttttcaacg tatagtagaa cgctatccgt agaacttgat
     4741 ttggtttcga cgctggattt ctcacctgaa aataccagta gttgatgcta aaggaaactg
     4801 ataaacaagc aagtaaaaca aacaacggaa tcaaccattt tccataagaa aaacgtataa
     4861 aaaagaaaca atcaaaaatc attatcacac taactatcca agcaaatagc caataaaaaa
     4921 atccctttag ccaaaattgg cgaaacgtct ttatgaacaa acgtactggt gaaaaacttt
     4981 tttcttttgc taattcttct gtcataccta tcatcacgat gcaa
//
LOCUS       DBNICI010000171         4974 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.175, whole genome shotgun sequence.
ACCESSION   DBNICI010000171 DBNICI010000000
VERSION     DBNICI010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.175"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(240..606)
                     /gene="ssrA"
                     /locus_tag="AC1ZCC_002497"
     tmRNA           complement(240..606)
                     /gene="ssrA"
                     /locus_tag="AC1ZCC_002497"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(777..1550)
                     /locus_tag="AC1ZCC_002498"
     CDS             complement(777..1550)
                     /locus_tag="AC1ZCC_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIQ1630716.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1694..2044
                     /locus_tag="AC1ZCC_002499"
     CDS             1694..2044
                     /locus_tag="AC1ZCC_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HIQ1630717.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2025..2741
                     /locus_tag="AC1ZCC_002500"
     CDS             2025..2741
                     /locus_tag="AC1ZCC_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIQ1630718.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2741..4189
                     /locus_tag="AC1ZCC_002501"
     CDS             2741..4189
                     /locus_tag="AC1ZCC_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIQ1630719.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4260..4769)
                     /locus_tag="AC1ZCC_002502"
     CDS             complement(4260..4769)
                     /locus_tag="AC1ZCC_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630720.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4759..>4974)
                     /locus_tag="AC1ZCC_002503"
     CDS             complement(4759..>4974)
                     /locus_tag="AC1ZCC_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630721.1"
                     /translation="ILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSG
                     CCLILSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1652 a          880 c          984 g         1458 t
ORIGIN      
        1 gtttttcctt ccatcaacat aatgttcacc tcaaaaagag tatacgaact aatgttcttt
       61 ttgtaaagta ggaatatcat tgataatatg gatggaacat atatattaaa aagtgttctt
      121 tttgaatgct attttgaatg ccattttcat tcatttaacg aaaatctgcg aaaataaaaa
      181 atgctcaaac ccttataaaa taaggattta agcatctagc gaaatctgct gaaatctcac
      241 aatggagacg gcgggagtcg aacccgcgtc caaacacatc gccacttaaa aatctacgtt
      301 catagtcaac tcatttaagg attcacttta cagcttgccg agtgacaggc attctgtatc
      361 gctagtctga tgttctcttt ttcaacttac agacggaaag ttgaaacgta tcccactaaa
      421 gttaggaccc ttactcgagc acatgggcga tgccgagagg atctacgcta actgttttta
      481 ggcagctaaa gcgtaagagt tgttttcgtt tttagcagtt atatttaact gtaacgtttt
      541 tacgtagacg taacctacga aacgcaattc aagctcgact gtgcctgtcg aatccgtaac
      601 gtccccgtta gctaacaagg gttacgtgta tttttcatac aaaattagta tagcatatat
      661 tgggataaat tcaataaaaa agcgaatcat atcccattat ttatataata actgcccctt
      721 tcttaagaaa aaataagcga ttttcttaag aaaagaggta gcttcaaaaa aagatcttaa
      781 atcagatcaa aatacttcaa tgcgtggaca attccgcctt ctgtattttt ctttgtgata
      841 aaggtagaaa gttcttttaa tccgtcatag gcgttgccca tagcgatttt gttgtcgcaa
      901 gcttccaaca aggcaaaatc attcggacca tcgccaaatc caaaagtagg aactttttcc
      961 agctgaagtt ctttttgcag acgtttgaca cctgcccctt ttgagattcc tttcttcact
     1021 gtatcgatcg agtaaggacc attccgataa aaagttagct caggaaaatg ttcgtgatag
     1081 tattcgtcac ctgattgaga taagatcagc aacatattta ctgtgttttc tttaaagcct
     1141 aaaggatcaa tttccggaac atctgaatga atgaagtcgt aagcattgat cattgtttgg
     1201 ctatgaccgg tacaccagat tttctgatcg ttatagtaag aaagttcatg accgttcaac
     1261 tgtacatgat catacatttt ttgacattct tctgaagaaa atgtttgaga atagatttct
     1321 tcgccgtcta ctcgaatgaa agaaccattc atggtgaccg cagaagtaat gcccgcatct
     1381 tccatgatag agtggatttc tgcttctgta cgtcctgtag cgatgacggg gagaacattg
     1441 ttttctttta atgcgttcat agcttttgcc acttcgggtg tgatttttga tttcgcatct
     1501 agtaatgttc catcaagatc aaagaaagtg attgcacgat aatttgacac aaataaaact
     1561 ccttttttat tggaataagt ttatcttaac aaagaatgaa caaaagacaa acctttcgtg
     1621 aagaattcct gaacatattg gttcagtcaa caatttagag tatactataa gaagagaatt
     1681 taggagggaa agaatgattt ttttcagtat tggtatctta ttcttaataa ttggcagtct
     1741 ttttcggatt ctgccctcaa aaggaaatct gcctttttat gggtatcatt cgcctcttgc
     1801 cgcaaaaaca gacgctcatt ggcggctggc tcaaaagaca agcggaaact ggttttttct
     1861 tatgggctta ctgatggcat tgatcggcta ttatttaaag acatctgggc atacgaatta
     1921 ttttctgatt gaaatgcttc tgctagtatt tccaattatg cctattttta ttatgacaga
     1981 aaagaaatta cagaaatatg acctggaaac tggagggaac gataatgaat attttaatga
     2041 ttgaagataa tcaatctgtt tctgaaatga tgcagatgtt tttcttaaat gaaggttggg
     2101 aagcaacttt caagtatgat ggaaaagaag ggctcgatgc ctttttagaa aacccacaac
     2161 attgggatat gattacacta gacctgaatt tgcccacact ggacggtatg ctagtaggaa
     2221 gagagatccg caaagtttca agtactgtgc cgattatcat gctgacagct agagattctg
     2281 aaagcgatca ggtgatcggc ttagagatgg gggcagatga ttatgtcaca aaacctttca
     2341 gtcctctgac gcttattgct cgaatgaaag ctttgcatcg ccgtagtgag ttggcagaag
     2401 caagagaaga agcagtagtt tcagatgatc attttgatat cgagactagt catttcaaga
     2461 tgaatacgaa aacacgagaa gcgtatctgg acaaccggcc aattgaagga ctgacgccta
     2521 aagagttcga tctattgttt actttagcaa aaaaaccacg tcaagtattc tctcgggagc
     2581 agttgcttga attagtctgg gattatcagt actttggtga tgaacggaca gtggacgccc
     2641 atatcaagaa attacgacaa aaaatcgaaa aggtcggacc acaagtcatc caaactgttt
     2701 ggggagtcgg ctacaaattc gatgattctg gtgttgccta atgcgttatt tgtatcagca
     2761 actcttagct ttctgcggaa tggtcgcgtt gatcttattg atcgttggag tttcttttac
     2821 acaactgaca aaacaaacga ttgaagaaaa caattaccag caactgtttg gatatgctga
     2881 atcagttgaa aagacaacgc aaacctatgc cgatacgctc ccgcagttta gtcaagatca
     2941 ggcgtttcaa aatgcattgc tgctgacaga gcaagtactg acagaacaaa atgtgaactt
     3001 tatatttatc gataaaaacg aacgtgtcat ttatccgact aatacagctt tactgaattt
     3061 taccttaaca gctgatcaat ggaaaaatct gaggaatgga cgtcaagaaa aattcacatc
     3121 gaataaaaat attttaggac aagcacaggc gacctcttac gcacttgtgc catttaattt
     3181 gaatcatgaa ttctatggag cacttgtagt tagtcaacct gcgcgaaata tcgataacag
     3241 cgttcgtcca attacactga acctgttcaa aggatttatt ttttccagta tcgttgcagt
     3301 tattgccagc tatgcttttg cagcattcca agttaagcgt atcaatcggt taaggaatgc
     3361 gacgaaagaa gtgacgaatg gtaattttga tgtacagctc cccgtacatg acaaagatga
     3421 atttgatgag ttagcagatg atttcaataa aatgacgaat tcattgaaag aatctcaagc
     3481 agaaattgaa gaacaagaaa atcgtcgaag acaatttatg gcagatgctt ctcacgagat
     3541 gagaacacct cttacgacaa tcaacggact tctggaaggg ttagaataca atgcaatccc
     3601 tgaaaatcag cgagaaaacg cgattaaatt gatgaaaaat gaaacagagc gtctgatccg
     3661 tttggtgaat gaaaacttgg attatgaaaa aatccgtacg aatcaaatca gtatggtcat
     3721 taaaaaattc aatggaacag aaacattgaa aaatattgtt gcacagttag aagctaaagc
     3781 agaagcagca ggagatacat tgacattgaa agccgttgat gatattgatg tttacgctga
     3841 ttatgaccga tttgtccaaa tcatggtcaa tattattcaa aacgcgatac agtttactga
     3901 aaacggccaa atcacgatta ctttagaaaa aggctatctt gaaacgatca tcacgattga
     3961 agatacggga attggaatgt ctgaacaaca aatgaaaagt atctgggatc gttattataa
     4021 agtagatcct tcaagaaaaa atacaaaata tggtgaatca ggattaggtt taccaatcgt
     4081 gcagcaactt gtacgcttgc ataaaggaaa attagaagta gaaagcgaac taggaaaagg
     4141 aacaaaattc gctgttcgtt tgcctgacgt agaaatcaaa aaagagtaaa aaacaagcgg
     4201 tataaaagat catgaatcga tcttctatac cgcttgtttt ttactataaa atgattgatt
     4261 tagcccgcgg gctctaaacg atcaatataa ggactttgag caatagtagc atcatgagta
     4321 aagagtaaga taccttgatt ttcttcatga gctaaataag aaagcaagtg gagtagctct
     4381 tggatctcca atacactaag ttcatcaaat gtatcttcaa taacaatatt gtttcgcttg
     4441 gaaagcaagg catgaagtaa ttgcagcttt actttttcaa aagacgtcaa ctcagctgcc
     4501 gcacaattaa gcaaggaagg agagatatca agtttttctt gataggccaa acgatcttgt
     4561 ttgatttctt tttccggtac atccaacagg acatggtctt taagattcag ataaggaaac
     4621 atcggccaat ttttttggat gatcgcaaaa ttatccagtt ttgcaacaag aacctctttt
     4681 ttcaagtgct ccaatatttt atgctgtttt tcgagaggac acaaaataac ggtcaagtgt
     4741 tcagcatgaa tcaaatattc aggtttcatt acgtccaggc ctcctacttt gcaaatgatg
     4801 gataaaaaaa gtgaaaggga atagactaag aatgagacag catccactga gcccaataag
     4861 agtttgggca aatcccttaa gtatatctgt gaaaaaaaga tcattttgga aatcgatggt
     4921 aaaaaaagtc tgttgatcca tcggaattgt aagtccgctt accgaagtca agat
//
LOCUS       DBNICI010000172         4925 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.176, whole genome shotgun sequence.
ACCESSION   DBNICI010000172 DBNICI010000000
VERSION     DBNICI010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.176"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            84..971
                     /locus_tag="AC1ZCC_002504"
     CDS             84..971
                     /locus_tag="AC1ZCC_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HIQ1630722.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1111..2037
                     /gene="cysK"
                     /locus_tag="AC1ZCC_002505"
     CDS             1111..2037
                     /gene="cysK"
                     /locus_tag="AC1ZCC_002505"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HIQ1630723.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2273..2719
                     /locus_tag="AC1ZCC_002506"
     CDS             2273..2719
                     /locus_tag="AC1ZCC_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIQ1630724.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2992..4344
                     /locus_tag="AC1ZCC_002507"
     CDS             2992..4344
                     /locus_tag="AC1ZCC_002507"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HIQ1630725.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4515..4802
                     /locus_tag="AC1ZCC_002508"
     CDS             4515..4802
                     /locus_tag="AC1ZCC_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HIQ1630726.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1694 a          813 c         1030 g         1388 t
ORIGIN      
        1 attaaaagta taatttaatt aattaagttt gttttaagaa gagaactttt tattaagttt
       61 ttttattttg aggaggaatt tgaatgcaag agctgaagac ccgcagtgcc cggcaccata
      121 gacctaaaag aaaaatggtg atcaataaaa aagcgattgc tcctttaatt ggaacgagtc
      181 ttttagttgg gccgatgatg gttacagcgg taccgcaaca tgttcatgca gatgaacaag
      241 gatctcaaac agtcgctgat cctcaagcgt ttatcgatca aatcggctgg tctgcgtcag
      301 aagttgctgc aagtaatgac ttgtatgctt cagtcatgat cgctcaagcg ttgttggaaa
      361 gtgcatatgg cacttccgga ttagctagtg cgccaaatta taatctattc ggtgtgaaag
      421 gaagctataa cggacaggtc gtttatatgg caaccaaaga atatgtaaac ggacaatggg
      481 taacgatgaa tgagccattc cgaaaatatt cttcttattg ggaatccttc caagaccatg
      541 cgaatgttct tcgttcaacc agtttttcca cgggcgatta tcattatgct ggcgtgtgga
      601 aaagcaatac aacaagtttt tatgatgcaa cagcgtactt gacaggtcgt tatgctaccg
      661 atccaaatta tggggccaaa ttgaactcat tgattaatac ttataattta acccgttttg
      721 atacgccatc aacaggttcg gctacaacaa ccacaagtac aacaacaacg actactacga
      781 cgacttcaac gtcatcatct tcaacaacag atgtaaatac aaataatggt cagacatata
      841 cagtcgtgtc aggtgatacg ctatgggata ttgcacaaaa atttggcatg tctgttgatg
      901 atctaatggc gaaaaatggc atgtctatgt ccagttattc tttattagtt ggtcaagaac
      961 taaacgtttg atttagataa aaaggcaaag aaattaccat atatggtata tctttgtctt
     1021 ttttttgcta gtcaaagacg aatcactcgc cataaatgaa cagattgtat tatgataagt
     1081 aagaagagca aagacaagga ggaattgaag atgacagcta tctataactc agtcaccgaa
     1141 ttgattggca aaacgccaat cgtcaaatta aataaaatcg taccagaaga ttcagcagat
     1201 gtttttgtaa agcttgagtt ttttaatcca ggaggaagtg taaaagatcg tattgcttta
     1261 agtatgatag aaaaagcaga acacgatggt ctactgaaac caggtgatac tatcattgaa
     1321 cctacttctg gaaatacagg aatcggattg tcgatggtag gagttgcaaa aggatataaa
     1381 gtaatcatcg tgatgcctga gacgatgagt atcgaaagac gtttattgat gaaagggtac
     1441 ggcgcagagt tgattttgac tcctggtgcg gatggtattt ctggatcaat cagagaagca
     1501 gaacgtttgg caaaagaaaa cggctacttt ttaccattac aatttgaaaa tgaagcaaat
     1561 cctcttgtgc atgagaagac gactggtcct gagatacatc aagcatttgg agtaaatggc
     1621 ttggacgcgt ttgttgcagg tatcggtaca ggaggaacga tcactggcgc tggccgagaa
     1681 ttaaaacgtg tgtatccgaa aatcgaatta atcggggtag aaccagcaga atctgctatt
     1741 ttagaaggga aagaagcggg acctcacaaa atccaaggaa tcggtacagg ttttgttcct
     1801 aaaacactgg atacttctgt gtatgataaa gtcctttcta ttagtggaga cgaagcgatg
     1861 gaaacagcta gagaagttgg gcgaaaagaa gggattttag taggcatctc atctggcgct
     1921 gcaattgctg ccgcgctaaa agtcgcaaaa gaattaggaa aaggcaagaa ggtattagct
     1981 gtggttcccg acaatggtga acgttattta tctacagccc tttaccaaga gatataaggc
     2041 acttggtttt tctccagata ggtttatgta aaaaactttt aaagatgaaa agaacacaca
     2101 aaaatttatg atgagaaaaa ttatttgaat agagacgtag aatagtgaac gcgactgttt
     2161 tacgtctttt ttcattaaaa tacaataaaa ttgaattttc ttttcatgtt aagtggattt
     2221 taaaatgtaa aaatagtatg ataagacgag aataacaacg tggggtgttt acatggaaaa
     2281 taagttagtc gaagaggtta tcgaaaagtt aaaaaaagag aacattcgaa tcacacctca
     2341 acggtacgcg gttttagaat atttgattga acatcattcc catccaacgg ctgatgagat
     2401 ttataaagca cttgaacatc ggtttccaaa tatgagtgtt gcgactgttt acaataacct
     2461 tcgcttattt acttcgattg gatttgttca agaaatgaag tacggcgatg cttctagccg
     2521 atttgatttc agttcaaagc gtcattatca tgtaatttgt gaaaactgcg gtaagattgt
     2581 tgattttcat tacccagggc ttgaagatat agaaatggct gcaagcagac tgactgattt
     2641 tgagattaat gaacatcggt tggaattgta tggactctgt ccggattgta aagagtcagc
     2701 aaaaaaggcg caaatgtaaa aacagtttct acaagtgtta tacctaaaaa aagctagctg
     2761 tgtaaaaaaa acgctagctt ttttattttt ttgtctttta ttcacaatct tagatataaa
     2821 agtgaatatt attaaaataa gaatcttgta gatattgggt tcttatgctt gaaaaggaat
     2881 gaaactacaa acaaaccttt gtgaattcac aagcattaaa ctgttgtatc cgttttattt
     2941 ctgttatagt agttttgtcg aaggacaaaa tgttaatcgg agggaccata aatgaaagta
     3001 gtagtaattg gctgtactca tgcaggtact gcagctgtaa aaagtatttt aaaaaatcat
     3061 ccagaagcag aagtgacagt atatgaaaga aatgacaata tttctttctt atcttgcggt
     3121 atcgcacttt atgtcggcgg agtagtaaag gacccagcag gcttgttcta ctcaaatcct
     3181 gaagaattat cttcattagg agcaacagtt aaaatggaac acgatgtaac agacgtcgat
     3241 acagaaaata aaacagtaac ggcaaaagat ctgaaaacag gagaaacaga aacggtttct
     3301 tatgataaat tagtgatgac aactggttca tggcctatta tcccaccgat tagtggaatc
     3361 gatgcgaaaa atgtactcct atgcaaaaac tacaatcaag caaatgaaat tattgcacaa
     3421 gcgaaagaag caaaacgtgt agtaattgtt ggtggtggat atatcggtat tgaattagta
     3481 gaagcttttg tcgaatctgg aaaacaagtg acattgattg atggattaga tcgcatcttg
     3541 aataaatatc tagataaacc attcacagat atcttagaaa aagaattgac agaccgcggt
     3601 gttactcttg cgctaggcga aaatgttact gaatttatca cagatgaaga aggaaaagtg
     3661 aaacaagtag ctacaccaac agatacattt gatacagata tggtcatttt atgtgtgggc
     3721 ttccgtccaa atacaaaact gatcgaagga aaagtagata cactgccaaa cggggcaatc
     3781 aaagtgaatg aatacatgca gacaagtaat ccagacattt ttgcagctgg agactctgca
     3841 gtagtcaact acaatccaag tggcacgcaa aactatattc ctttggcaac aaatgccgtt
     3901 cgtcaaggtt tgcttgtggg aaataacttg acagatcata aaatggcgta tcgtggtact
     3961 caaggaactt caggacttta cctatttggc tggacgatcg gttcgacagg tgtgaccaaa
     4021 gaaagcgcaa ctttgaacga tctagaagtc caagcgactg tatttgaaga taattatcgt
     4081 ccagagttca tgccaactac tgaaaaagta atgatggagc tagtctatga aaaaggaacg
     4141 aatcgtatcg taggtgctca gttcatgtcc aaatacgaca ttacacaatc agcaaatacc
     4201 atgtctttag ctgttcaaaa taaaatgact gtagaagatc tcgcactttc agatttcttc
     4261 ttccaaccac attttgaccg cccatggaat tacttgaacc tacttgcaca agcagcgcta
     4321 ggcgaaatcg aaactgcaca ataaagaaaa agggttgtga cacaattttg tcacgaccct
     4381 ctgtcttttt ttgtgaaaag tacaattagt tgtctttctt cgaatttttt gtcacaataa
     4441 gcagcaaaga taaagctgtt acataaaaga acaagcagct ttatttgatt ttgaaaagaa
     4501 aggtgagaaa gaagatgaat aaagaatatt ttgtccaaca agatgcattt ttagaagatt
     4561 ataaaagtgc ttcgaagaaa ttaacgatca atattgatga gttggcagat caagtactct
     4621 ctcagttgaa tgaaacgcaa gaggaatatt acacgatgcc agcaaatgac gccaaagata
     4681 atgtttctca tcgttttcct ttcagtagac gcatctactc agatgaaaat gacggcacat
     4741 tgaatacaga gtacacatat gaaggtcagc catatgtgct agaaactgat gatgatcttt
     4801 aaagacagtt tttgaaaagg ccgctcatcc ttaagtgatg acaaaaaatc atcacaatta
     4861 ccgatgactg tctctttact atcttttaat cagaaaaagg ggttatgaca agctttgtca
     4921 acccc
//
LOCUS       DBNICI010000173         4920 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.177, whole genome shotgun sequence.
ACCESSION   DBNICI010000173 DBNICI010000000
VERSION     DBNICI010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.177"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            871..1287
                     /locus_tag="AC1ZCC_002509"
                     /pseudo
     CDS             871..1287
                     /locus_tag="AC1ZCC_002509"
                     /inference="COORDINATES: protein motif:HMM:NF012385.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            complement(1328..1639)
                     /locus_tag="AC1ZCC_002510"
     CDS             complement(1328..1639)
                     /locus_tag="AC1ZCC_002510"
                     /inference="COORDINATES: protein motif:HMM:NF012311.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIQ1630727.1"
                     /translation="MPSAWEETKGSKDVKVAVIDSGIDYQHEDLSGNVDESLGYDFFY
                     FFQENNNSEYSDYIYATLVTLGETSIALLKFLLSLKRVFSIICFFLKYLVPQYLGYGY
                     V"
     gene            complement(1683..2144)
                     /locus_tag="AC1ZCC_002511"
     CDS             complement(1683..2144)
                     /locus_tag="AC1ZCC_002511"
                     /inference="COORDINATES: protein motif:HMM:NF047111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIQ1630728.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNR"
     gene            complement(2404..2952)
                     /locus_tag="AC1ZCC_002512"
     CDS             complement(2404..2952)
                     /locus_tag="AC1ZCC_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630729.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(2953..3810)
                     /locus_tag="AC1ZCC_002513"
     CDS             complement(2953..3810)
                     /locus_tag="AC1ZCC_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIQ1630730.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYVEVE"
     gene            complement(4096..4383)
                     /locus_tag="AC1ZCC_002514"
     CDS             complement(4096..4383)
                     /locus_tag="AC1ZCC_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIQ1630731.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(4373..4702)
                     /locus_tag="AC1ZCC_002515"
     CDS             complement(4373..4702)
                     /locus_tag="AC1ZCC_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIQ1630732.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(4779..>4920)
                     /locus_tag="AC1ZCC_002516"
     CDS             complement(4779..>4920)
                     /locus_tag="AC1ZCC_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family
                     toxin"
                     /protein_id="HIQ1630733.1"
                     /translation="SFEKLKPTHEGRYFRRLNRQHRVVYKVDEDEHVVEIYSARTHYE
                     SQ"
BASE COUNT         1500 a          929 c          702 g         1789 t
ORIGIN      
        1 aaattgacaa caaggcaaac gtgtattgaa acgcatacat tgttattcaa cttatttagg
       61 tcctgcctgc attaccagtc acatgcctgt ttaagttata ttcaatttaa catattagaa
      121 tgtaaacgta aataataaaa tgatttttta atcatttttt cattttattc gttcaatata
      181 gttttcgtgt tctaaaagtt ttgtcttttt tctaagaata tcttccaaca tatataactc
      241 ttattctctt ctatcttcta gcagatgttc tgtcttttta agacctagta taaaaagaat
      301 gggttcattt tttctttctt gcaggatgag gttttttata taaactttat ttgatataaa
      361 gaaaaggcat tcataattgt gctatactac taaaaaatga taatacagtg aattagatta
      421 gcgctttatt aaattaagag gacagcatat cgatattgct tccttttttt tgctatttat
      481 ttaagggata gaagtttaga aaggactgtt tataagatga aaaatgatat agtgacttat
      541 gcaaacacaa aatgtgaaga aattggcatc agtaatttag aagagtgctt ttttgacaca
      601 aatgacctgt cgcaattttt aagaacggga agaacaaatg ccagtaaact tgccaatgac
      661 ttagttgcaa agaacgttct atttaaaatc aatactcgtc cagtgttatt tgccatcaaa
      721 gagcgctttg aagagcactt tttgattccg gtaaaagatg tatacgaaag tatatctgaa
      781 ttaaaaacag acttggagcc caattctaca gattccattc tacaaaagat gattgggtat
      841 gatatgagct tatccgatgc ggtggaacaa atgaaaactg ctgtcttcta tcctggaaga
      901 gggcttccta tgatgatcac tggagatact ggagttggaa aaagtttttt tgttcatact
      961 gcttatcagt ttatgaaaaa agtgaacgtt attcatgaga atgctccttt taaagtactt
     1021 aattgtgcgc aataccataa taatcctgaa ttattgtcgg ggatactatt tggctatgtt
     1081 aaaggtgctt ttaccggcgc agatgaagat cgaaatggat tgttgcaaga tgcagacggt
     1141 ggagtactgt ttttagacga agtacacaga ttgcctcctg aagggcaaga gaaactattt
     1201 gtttatatgg atggcggaac ttattctaga gtcggtgaaa ctatccatca cgcctccaat
     1261 gtacgtttag tttttgcgac tacagaaggc aaagaggatt ttctggaaac ctttatgcga
     1321 agaatcccta tacatatcca tatcccaaat attgaggaac gaggtatttt agaaaaaaac
     1381 aaattatcga aaatactctc tttagcgaaa gcaaaaactt tagcaaggct attgatgttt
     1441 ctcctaaagt cactagtgtt gcataaatat agtcagaata ttctgaatta ttattttctt
     1501 gaaaaaagta aaaaaaatcg tatcccaaac tttcatctac atttccagaa agatcttcat
     1561 gttgataatc aattcctgaa tcaatgaccg ctaccttcac atccttagaa cccttggttt
     1621 cttcccaagc ggatggcatg ctaatctctc ttaaattagc ttgtttactc caattaggat
     1681 cgttatcgat ttcctaaaga atcagttgta tttggctcct caacatcatt caacggctct
     1741 agttcaacca catagtttgg ctctgcataa ttgaccatat cagaagcatt aaatgttctt
     1801 actgcctctt ccacactaat ttcttcgttc aatttcacta ataatacttg atctcgctta
     1861 ttataatcgc tttcatttgt cctaagagtc aaatcttttt ccaccataac ttttacaccc
     1921 aataaatttt gcggtttatt tttctctgtt gaaattcttt gtgtagcctt taaactgaca
     1981 attagctcat tgggtacata ctctatttga gagtcatcta attggctcat aatcctttct
     2041 ttttggttag tatctgcagt tacttgttta gtatgagcca tgaacccaaa catactcacc
     2101 ccaacaacca atcctaaata tattttcttt ttaaactttt tcatctacat ctcctcctaa
     2161 tagttttatt ttagagttag ctctattact tatttactaa ctcaacaaca gattatcata
     2221 cacttattaa tgaaatcaca atatctcatt aaaacacacc ctaaaatcat aaccgttcct
     2281 atgctcattt tttggatttt ttaatcacgt aaccagaaaa ataaatcccc tctatttcac
     2341 gcaaatcaaa gaagaactaa gtaactaccg tttaaaggta gttacttagt tcttctttga
     2401 ttattatcta actttattat tcattcccca ccctttgcgc agtgagtctt cggtatcttg
     2461 ttttatctga ctggtagttc ttattatttc tccacgatca tctaattctg gaacaattgg
     2521 atatttttct ctgaacaagt ctagttcttt caccacgtca ttaaacaaat cttgtttatc
     2581 ttcttgcgta agattctgtt taatatactc atctaaagct tttctaagca attcatctct
     2641 tgtcatatta gtctcatact tttctactaa aactctggaa gtcgcattta acaaagtcga
     2701 cagttttaaa ggatgcttgg tggaataaac cttatcaaac tttctgattg gtgcgccaac
     2761 tttttttgtt cttcctctac tctgtctttc aacattggag gaagttttta tttcttgaac
     2821 ttggtgcact tctaattgct tattttcaga tggtgtaatt gcttgctgct ttttctttgc
     2881 ttctgcttct ttttttattt tggcgagtcc tttgttgttc ccaaaaatat caagtccttt
     2941 gatgtcagcc aattactcca cctccacata ccaattctcg tgttcttcca attctttcaa
     3001 aatactgata aataattcgt ggcttttgcg atcccattgt tttaagagac ctttctttgt
     3061 cttctccatc gtaatacctg atccgtcgta tcgttttaag cgcgcatttt tgagtacaac
     3121 atttttaatc aaatgttccc cataaatctc ttgggcttgt ttatacattt cctgatctac
     3181 tgcgtcaccg ctttccaaca tgaaaggaat aacaccagca acatataaat ttgtatcaaa
     3241 ttctttcaca aaaaagtcca tatactcaag atattgcatc gcatttctca tcgattttac
     3301 ttgggtctgt aagacgacga ttgcccaatc cgcaatgtac atcgcggatt tactatatac
     3361 agaaatagaa ggtgggacat ctaaatagat gacgtcaaac tcatcaaata atggttctag
     3421 cttttctttc atatatttga attgggataa tgaatcatta tattggtctt tgatccaatc
     3481 atcatacatt tcaaaggaca tgtctgctgg aagtatatag aggttattga ttactttata
     3541 gacagcctct ttaggtggaa gattgtttat caacacgtca gtcacagtat tttcgtaaac
     3601 aatcggttca ccttctccgc ctccttcttc gttagtgaag ttcatatgcg ttaagttcat
     3661 cacatcagaa gtatttcctt gcatatccaa gtcaattatc aaggttcttt ttccttcatt
     3721 aactgctgat tcaaacccta acatttgcgc gcacgtactt ttacctacgc cacctttaaa
     3781 attaccgatt acatatttct tcgctaccac gttcctacct cttttctttt agcaatattc
     3841 atttgatact attataacat atttattttt agttactacc aaaaaaaaga tatatatgta
     3901 ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg aaatatattg
     3961 atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca gagaatactc
     4021 gcgactttag tcatgtagac gaatcagatt ttcgataaaa aagaagccgt ccttcttaag
     4081 aacgacaaca aatcactaga ttaatttttt caactgttca taaaagtttt catgcgttcc
     4141 acataacaat actggaataa ccttgttttc tataatctcg taagctactc tatatgttgt
     4201 gcccgcatat ttgaacccac tagaccaaat acctgataaa tccccagttt tttgttcacc
     4261 cttatatgga tcaatcgcaa tttcatcata aattaaattc aaaaattttt ctttcaatgg
     4321 cttctctttt agctttttta aatacttaat gacatgtttt gaaggaacga tttcataagc
     4381 caatttctgt tgccaactcc tctttagtca ttactttaga attagtaagt gtatcttcag
     4441 caatatctct aaaggaagaa tgcattttgt tttttctatt cttaaattct acaagaatct
     4501 catctttatc gtagccttct gcaattatat cagacaaaat atcttcgcta aaatcataaa
     4561 attctttttg tggttcaaca aattcataaa gaataccatt ctctaccaac tttgcttcaa
     4621 cttcgactcc attaggaaca ttaaaatctt taggtactgt caacatgatt gaattgcctt
     4681 gttttcttgt ttttgtaatc atttaaatcc ctcatttcta acagtatgta attacttagt
     4741 aaatacatta taccacgaga taatgtattt acaagtaatt actgtgactc gtaatgcgtc
     4801 cttgctgaat aaatttcaac cacgtgttca tcttcatcta ccttataaac aacccggtgt
     4861 tgtctattca atcttctaaa atacctacct tcatgtgtag gttttaactt ctcaaatgat
//
LOCUS       DBNICI010000174         4891 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.178, whole genome shotgun sequence.
ACCESSION   DBNICI010000174 DBNICI010000000
VERSION     DBNICI010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.178"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            35..2053
                     /locus_tag="AC1ZCC_002517"
     CDS             35..2053
                     /locus_tag="AC1ZCC_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIQ1630734.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    2123..2329
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2480..>4891
                     /gene="leuS"
                     /locus_tag="AC1ZCC_002518"
     CDS             2480..>4891
                     /gene="leuS"
                     /locus_tag="AC1ZCC_002518"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIQ1630735.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
BASE COUNT         1679 a          917 c         1057 g         1238 t
ORIGIN      
        1 taataataga taaattgaag ttggaggata gaagatggaa aacattgcaa gaaaagaacg
       61 gagacgttta gaacagacca aacgttttcg aaaagcaaaa cgaggggcag caatcgtggg
      121 aactgcgatg gtaggctgtt ctgtcgctgc cccattagta cagccggttc ctgttcaagc
      181 ggatgaaaca cctgttcagt tcggcgcaag aatcaataca gcggcattta tcacagaaat
      241 tgctacttat gctcaaccaa tcgctcaagc caacgatcta tatgcatcag tgatgatcgc
      301 acaagcagtt gtagaaagcg gttggggcag tagtgcgtta tctcaagcac cttattataa
      361 tctttttggg ataaaaggca gctatcaagg acaaaccgtc tacatggata cgcttgaatt
      421 tttaaataat caatgggtaa ataaaaagga gcctttccga caatatcctt cttttgctga
      481 atctttcaat gacaatgcct atgtacttag aaatacttca tttggcaacg gttattacta
      541 tgccggtact tggaagagca acacgaaatc ctatacagat gcgacagctt gtttgacagg
      601 gcgttatgcg actgatcctg gttacgcagg caaattaaat aatatcatcg caacttacgg
      661 attgacccaa tatgatacac cagctagcgg aaacgcaggc ggtggagcga ctacaggtgg
      721 caataatact ggaagtgcag gtagtactgg cggttcaagc aattctggta atacaaactc
      781 aggttcagca acttcgacca cttatactgt ccaatctggt gattctgttt ggggaatctc
      841 aaataaattc ggtatcacaa tggatcaatt gatccaatgg aacaacatcc aaaacaattt
      901 catttatcca ggacaaaaac tgacaatcaa aggtagccag gaaaatggct cttcgacgaa
      961 taattccggt aacaatacca attcttcagg aaatgctggt acatcaaacg gaggacaaac
     1021 aactggtgcg aaatacactg ttcaatctgg tgattctgtt tggaagatct caaatgatca
     1081 cggaattacg atggctcaat tgattgaatg gaacaatatc aagaacaatt tcgtttatcc
     1141 aggacaacag ctaatcgtat caaatggcgg ttcttctaat gctggagcaa atactggctc
     1201 aaatactggg aatacaagtg gctctagttc gtccagctca agttcttcta atacagcttc
     1261 aggaacgaaa tatacagtta aagccggaga ctctgtttgg agcgtctcaa ataaatacgg
     1321 aattacgatg gctcagctaa tccaatggaa caatatccaa aataacttta tctacccagg
     1381 acaacaactg actgtttcaa acagtggatc aacaaacagc acgtcaggta atacaggttc
     1441 gtcaaccaat acaaataaca gcagttcagt taatactggc agcagttcgt ctcaaggcac
     1501 ttataccgtg aaagcaggtg aatctgtttg gagcgtggca aataaaaacg gcatcacgat
     1561 ggatcagtta atcgaatgga ataatatcaa aaacaacttc atttatccag gacagcaatt
     1621 gatcgttaaa ggtggaactt cggcaaatac aaatacaggt tcaactactt ctgcagcgaa
     1681 accaaataca ccgaatacgt ctgctacgac aagtacatca tccactggta atacgatgta
     1741 taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac aaacatcata tcacgatgga
     1801 tcagttaatc gaatggaata atatcaaaaa taatttcatc tatccaggac agcaagtgat
     1861 tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca gttactggtt cgaaaaccta
     1921 tactgtgaaa tctggagaat ctgtttggag tgttgctgac agtcatggaa ttacaatggc
     1981 tcaactaatc gaatggaaca atattaagaa caactttatt tatccaggtc aaacgttgat
     2041 tgtagctaaa taaaatagct gttcgaagaa ataacgattg aattccacaa aaatatttgt
     2101 tactataagg ctacttaaat atgacaacac ttgaaaagga agtagtaaaa agactgaagt
     2161 ttcagagagc gcatggctgg tgagaatgtg cacggatgct tttgaactcg cctttgagtt
     2221 gcccttgctg aatacccgag taagcaagcg gcggtgacgg ccgttacaac gttcgaggcc
     2281 ttttcactaa ggtaaaatta ggtggtaccg cgtagattac gtcctataaa aagagcaatc
     2341 tttttatagg atttttttat ttcttttgtg aagtttgaga aaacaaagca aaaggaaata
     2401 aaaataaaca gattctgccg gttttttcgt tatatttata atagaaacaa gtgttttcaa
     2461 tagaacagga ggacagcaaa tgagttacaa tcataaagaa attgagaaaa aatggcaaaa
     2521 atactgggca aaaacgaata ctttcaacac ccatgatgat cctgaaaagc caaaatttta
     2581 cgcactggat atgtttccct atccatccgg tcaagggcta catgtagggc atcctgaagg
     2641 ttatacggca acggatatcc tttcacgtgt gaaaagaagt caaggataca acgtgctgca
     2701 tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa tatgcgctag atacaggaaa
     2761 cgatccagct gagtttaccc aaaaaaatat cgaaacattc cgacgccaaa tcaattcttt
     2821 aggatttagt tatgactgga atcgcgaagt gaatacaact gatccggagt attataaatg
     2881 gacacaatgg atctttacga agctttatga aaaaggtcta gcttatgaag cagaagtagc
     2941 ggtaaactgg gtacctgaat taggaacagt tatttcaaat gaagaagtga tcgatgggaa
     3001 aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg cgccaatgga tgctgaaaat
     3061 cactgcttat gctgatcgct tattggatga tctagaatta gttgattggc cagaaagcat
     3121 taaagaaatg caaagaaatt ggatcggtcg ttcagtagga gcaaatattg aatttaaagt
     3181 ggctggtacg gataaatcct ataccgtatt tacgacacga ccagatactc tattcggtgc
     3241 aacttactcg gttcttgcac ctgaactaga tcttgttaga gaaattacga cccctgaaca
     3301 aaaagctgct gtagaagctt atatcgaaga gacagctaaa aaatcagatc taaagagaac
     3361 ggatcttgca aaagaaaaaa caggagtatt tactggtgct tatgcaatca atccagttaa
     3421 tggcaaggaa atcccaatct ggatcgccga ttatgtatta tcttcatacg gcacaggagc
     3481 catcatggct gttccagcac atgatgaacg tgactttgaa ttcgctcaaa ctttcggact
     3541 agaaattctt ccagttatcg aaggtggaga tgttgaaaaa gctgcttaca cagaagatgg
     3601 tacgcacatc aactctgaat ttctagacgg catgaacaaa caagaagcaa tcgataaaat
     3661 gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa gtcagctatc gcttacgtga
     3721 ctggctattc tcccgtcaac gttactgggg tgaaccaatc ccaatcatcc attgggagga
     3781 cggaacagtc acagctgtcc cagaagaaga gctaccatta cggttgccta agacaaaaaa
     3841 catcaagcca agcggtactg gagaatctcc attagcaaat atcgaggagt gggtcaatgt
     3901 cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg aatacaatgc cacaatgggc
     3961 aggaagctca tggtattacc tgcgttatat cgaccctcat aacaaaaaag aactagcaga
     4021 ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac attggtggag cagaacatgc
     4081 ggtacttcat ttactatatg cacgattctg gcataaattc ttatacgata tcggtgttgt
     4141 tcctacaaaa gaaccattcc aaaaactata caatcaagga atgatcttag gcgaaaacaa
     4201 cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct gatgatgtgg tcgaaactta
     4261 cggtgcagat acactgcgca tgtacgaaat gttcatgggg ccattagatg cttcgattgc
     4321 ttggagcgaa aatggcttag aaggaagccg taaattcttg gatcgtgtat ggcgtttgat
     4381 cgttgatgag aacaataaaa tgcgtgaccg tatcactaca ttgaatgatg gcaagcttga
     4441 taaagtgtat catcaaacag tgaaaaaagt gacagaagac tatgaaaatc ttcatttcaa
     4501 tacagcaatc tcccaattga tggtctttat caatgaagcc tataaagtgg acgctctgcc
     4561 ttatgaatat atcgaaggat ttgttcaact actcgcaccg attgctcctc acatgggtga
     4621 agaactatgg gcaatccttg gaaatgatgg tggtatttct tacgcaccat ggccaacata
     4681 cgatgaagca gcacttgttg aagacgaagt agaagttgtt ttccaagtaa acggtaaagt
     4741 tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat gagctagaaa aagcagcatt
     4801 agccaatgaa atagtgcaag aatatattga aggcaaaaca gtcagaaaag ttattgcggt
     4861 accaaacaag ttagtcaata tcgttgctaa t
//
LOCUS       DBNICI010000175         4886 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.179, whole genome shotgun sequence.
ACCESSION   DBNICI010000175 DBNICI010000000
VERSION     DBNICI010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.179"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(20..904)
                     /gene="rsmA"
                     /locus_tag="AC1ZCC_002519"
     CDS             complement(20..904)
                     /gene="rsmA"
                     /locus_tag="AC1ZCC_002519"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIQ1630736.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            complement(897..1466)
                     /gene="rnmV"
                     /locus_tag="AC1ZCC_002520"
     CDS             complement(897..1466)
                     /gene="rnmV"
                     /locus_tag="AC1ZCC_002520"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HIQ1630737.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            complement(1459..2235)
                     /locus_tag="AC1ZCC_002521"
     CDS             complement(1459..2235)
                     /locus_tag="AC1ZCC_002521"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIQ1630738.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            complement(2390..3712)
                     /locus_tag="AC1ZCC_002522"
     CDS             complement(2390..3712)
                     /locus_tag="AC1ZCC_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIQ1630739.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            3887..4474
                     /locus_tag="AC1ZCC_002523"
     CDS             3887..4474
                     /locus_tag="AC1ZCC_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630740.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
BASE COUNT         1387 a         1029 c          839 g         1631 t
ORIGIN      
        1 tctgtcacaa cctctttttt tactgcatat tttctgacat cgcattgctt aatgcggcga
       61 attcttgtaa tgacaaagtc tccccacgac gttttggatc gattccagct gtttccaagc
      121 tttttgctaa ccatgctttt gtctggtcat ctttgccata cgaatgctgc aagttattcc
      181 aaagcgtttt tcttctttgc tgaaatgccg cttttgtcaa tcggaaaaat gctttttcgt
      241 ctgtcacttc gactgccggt atatctcttc ttgtcagttt caaaatcgct gaatctacat
      301 ttggctgagg gacaaatact gtttttggta cgatgaatgc taaactggct tccatataat
      361 attgaacagc aatcgataat gaaccataag ctttcgttcc tggttcagca gagatacgat
      421 cagctacttc tttttgcatc atgacgacta gttcatccac aaccaaatct gattcaagaa
      481 aatgcatcat gatcggtgtc gtaatatagt aaggtaaatt cgctacgact tttaacggaa
      541 gctcctcatg aaatgtttct ctaacagctg ttgacaaatc ggtctttaaa atatcttgat
      601 gtacaatagt cacattgtca tagggttgca tcgtatcagc caatacaggg atcaaacgat
      661 catcgatctc aaatgctacg acttgttttg catgcttggc taattgttcc gtcaatgcac
      721 cgattcctgg tccaacttcg atcacgttcg tatgttcatc tatttgcgca gtttctacga
      781 ttttccttaa aatatttggt tcagtcagaa aattttgtcc taaacttttt ttaaatgaaa
      841 aaccatgttt caataaaatc tcttttgttc tagagggtgt cgcaatttca cgatattcac
      901 tcatgttgtt cctccactat tttcattgct tgggcgagtt ctttcggcgt aatacgaaac
      961 atcttcaaac gtttttccaa ttgtttgccg ttcgtgtaac cgatgcgcaa ctcgtcacct
     1021 agtttttctc gtttttcttt tgcttgtgcc ccagctatta aaccatagtc taaaaggtct
     1081 tgtctagtga tttcagaaac agccgtttgg gaaatgtctg gcgtcacgac ctttttcaat
     1141 gcttctaaaa tagcagcatc acttgcatgc tcgacaccaa ggcttccctt tttctttggt
     1201 ttagcttgtg tgcgcggcaa aaaagcatgt tttgcctgag gaacagcttc cataatgatt
     1261 tttcttattt tttctcctga aaaatcagga tcggtaaaaa cgatcactcc acgagtctct
     1321 tgggcatgtt caatttgcat caggatctct tcattgattg cagaccctat tgtttctatc
     1381 gtatctgcat caactacttc ccgcagcctt cttgtatcgt cttttccttc aacgactata
     1441 atctcttgga tcttcatctt atccatgttc gcttagacca aataatcggt gcgcatttgt
     1501 ccatgtttgg gcagccactt cttctgtcgg aagattccga agttcagcaa tcttttctac
     1561 tgtataacga gtatagcctg gttcatttcg ctttcctcta aaaggaactg gtgccagata
     1621 aggggcatct gtttccacaa gtaatcgttc gattggtaca tttgctgcag cctcttggac
     1681 gtctaatgct tttttgaaag tgacgacacc gctaaatgaa atatgcatcc ctaagtcaag
     1741 aaatcgtttt gcccattcat aatcaccact gaaactatgc atgattccac cgatatctcg
     1801 aacatcttct tcttttagga tttgatacgt gtcttcaagt gcttctctag tatgaatact
     1861 gattggtagg ttcatttctt ttgcgatagc aatctgtctg cgaaatactt tatcttggac
     1921 ttctttcggg tcttccatcc aatgataatc caagccaatt tcccctaatg ctacaacctt
     1981 tggtaaagtc aattgttctt gtaatttatt ttcgatttct ttcgtataac tgccagcctc
     2041 tgtcggatgc cagccgataa tactgtatat atttggatag tcatggctta attgcaaaga
     2101 cttttcaatc gttggcgtat cgaagcccac gaccgccatt ttcgttaccc ccatttcttg
     2161 tgctcgctga atcgtttctg gaatatcttc attgaactgt tcagcattta aatgggtatg
     2221 agaatcaaat atcatgttta tctctccttt tcattcttac tttagcacat aagcactact
     2281 tgcagaagta gcaaaaatca aaatagaaga acttgtttta ttgaaataga aaaagcacga
     2341 agatctagag gacctagttc gtactttttc tgtctgttgt tgaaatattt tactcttctg
     2401 agcgtttttt ctttttttga taatagactg ggatacctat caaggtaaca cctataccga
     2461 tcaatgctaa tttcgtttgc gtcatcatcg tcatacctaa aatgaaaata gcaccgagaa
     2521 tcgcaataat cggaacgatt ggataaagtg gtactttata tggacgtggc aattccggcg
     2581 ctttttttct taaaataaag acaccgataa aaatcagtaa actgaatagc cacatcacaa
     2641 aaatcaacat gtctgttaaa aagtcgaaag aaccaaggct catcataata caagctactg
     2701 ctaactggaa aaccgcagga acataaggaa ccgtaaactt ctttgaaagc tttgtcagct
     2761 gtttactgaa aggtagctcg tcttctaaag ccatcgcata aggcacgcgg atcccagtta
     2821 gtgtataacc gttcaatgca ccgtataccg agatcaagat cccaatagtg accagctttc
     2881 caccgattcc tccaaaaatg acgtctgatg cttcagaagc tgcattcaaa ttaccggcaa
     2941 gatgatcaat cggcaaagtt ttcaaaaaaa caaaattgat caatagataa acgactgtta
     3001 caaaactcaa accaagaata attgctttag gcaaatcttt ttccggccgt ttcatttccc
     3061 ctgccattgc accgacacct aaccagccgt cataagcaaa caacgttgct aacaacgcac
     3121 tgctcaatcc ttcgacaaaa gtaacatctt ttccagcttc gattggaaat aactggacag
     3181 ttccttgtcc tggggtcaat aatccccata tgacaatcac agcaattggt attaatttaa
     3241 caatcaacgt cactgattgt acagaagcac cgacttttgt ccctaacaaa ttcaaccctg
     3301 tcacagaaac agcggtgatc actgcaatca acaaaagata atcggtagat aattgtaata
     3361 agttcgtcaa ttgtgtcgca aagataatcg ataatgcagc aatattagca ggaaagtaaa
     3421 tcaagctttg cgcccatcct aataaaaagc tgggcaattt tccatacgca gcttcaatat
     3481 attttacagc tccaccagtt tccgggattg ctgtagctaa ttcagccact gtcaatccag
     3541 cacagatcgt taagaaacca cccaaaagcc aagctagcaa tgtcaaactt gtagactgag
     3601 tactagcagt aacagcagca gttttgaaga agacgcctgc accgatcacg gttcccataa
     3661 cggtagctaa tgcgccaaac agattgattt ctcgttttag tttgcctttc attcccatcc
     3721 tctttcttct ttgaaatttg gtataatttt cagtataact gagaatattt taatttacca
     3781 ttcttttttc tccactttca gtgaaaaact ccctcttaag acggatgtat ttctttttta
     3841 tttttcgtat actaaaccta ccttttccac gaaggaggaa cagtttatgg attttaagcg
     3901 ttcttatccg aaattagcag attatttatc caaccgggtc gatttttccc gtaaagaaca
     3961 acagcagtcc cattggcatt ggttcacatt aattttattt ggattaatga ctgttgcaac
     4021 gttatcacaa aaactaatat tgctgacttt gttcatcgca atcgttgcat tgatcaaagg
     4081 tcctgtgatg atcttgtggg ggatgttgta ttcctttttg gttgggcttt ttccgcctat
     4141 cggtattttg ctttcagcga ttttcttttt attgaatatc ggaacattta caaagaactg
     4201 gcggatgacc ttagtcggga tctattttta tttctatcct tttggagtga tggcacttag
     4261 tgaagtcatg cattgggata atcattggtt tatagctggc tccttgcttt tagggttgat
     4321 cttgttacac gtgatgttga caaagctcta tcaacattat ggtatcggac gtacgatttt
     4381 ctggtatgtc ttttcgatcc catttgcact gcttactgct ctcttaccaa gccgtttgaa
     4441 gacaaaaata aaaggttacc ataaaatcaa gtgataggat ttttacaaaa taaagaaaac
     4501 aatcatcgaa gtcaaagccg atgattgttt ttttatctat attttcaatc acacatataa
     4561 gagcacaaac taattgaaca taaagtgata gaacttataa ttaacacatc aacgctactg
     4621 ttagatataa atatagatgt aataaagtca ttgcattcaa actttatgaa tgtaaggaca
     4681 ataaaaaaga gtaccagatg aatcaattaa aatctgcccg accctttatc attatgcttt
     4741 ttttctgttt ttacgattcc tattagtatt attctaaatt aacaaaaatc agcgaagctc
     4801 gtacttcttt ttctaggatt tattttggat agttctctgt ttctgtatta tatgattggc
     4861 ttcaagtaat aagaaggaat ttacga
//
LOCUS       DBNICI010000176         4867 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.180, whole genome shotgun sequence.
ACCESSION   DBNICI010000176 DBNICI010000000
VERSION     DBNICI010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.180"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(227..1114)
                     /locus_tag="AC1ZCC_002524"
     CDS             complement(227..1114)
                     /locus_tag="AC1ZCC_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1630741.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1111..1980)
                     /locus_tag="AC1ZCC_002525"
     CDS             complement(1111..1980)
                     /locus_tag="AC1ZCC_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1630742.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2117..3403)
                     /locus_tag="AC1ZCC_002526"
     CDS             complement(2117..3403)
                     /locus_tag="AC1ZCC_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIQ1630743.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3676..4254
                     /locus_tag="AC1ZCC_002527"
     CDS             3676..4254
                     /locus_tag="AC1ZCC_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIQ1630744.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4254..>4867
                     /locus_tag="AC1ZCC_002528"
     CDS             4254..>4867
                     /locus_tag="AC1ZCC_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630745.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFD"
BASE COUNT         1509 a         1001 c          829 g         1528 t
ORIGIN      
        1 gacaatcagg acagtcatcg gcaatatagc agcaatcact tctttaaaat tttttagcat
       61 gtatcctcct cctttactct ttaagtttac cttaaaatga aagagttttc gagcacaaac
      121 ttaaaacgtt atcatttaat ttttctcaac ataaaataac acacgtccca aaagtatctt
      181 tttacttgga aaaatgactt tgaaggaacg cgcttattgt gtttttttat tttacagagc
      241 cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa tacgatggca gttggtaaag
      301 tagcgatgac taatccagcc atgatcattc catagtctgg tgtataggaa gcacccatag
      361 cagataaaac aagcggaacc gtccgtttat caggagattg caaagctact aagggccata
      421 aataattgtt ccagctactc atgaatgtga tgattgcagc cgcggcatag gtatttttag
      481 ccgtcggcat atacactcta aaaaaaatac ttagttcttt tagaccgtct aatcgagcgg
      541 cttctactag atcttttgga aaagatttaa cattttgtcg aaagaagaag atcaaaaaag
      601 cagtgctaac agatggcagg atgacaacca agaaactgtt gattcccaaa accgttccgt
      661 tcaattgtga aaacattcga tataaaggga tcatcaatgc cgcaaaagga accatcatcg
      721 atagcagtag aatattgaaa attaggtctt ttcgtttact ttgataaatc tcaaatccat
      781 atcctgccat tgacgaaatc agcaatgcaa acacagtggt caccaaagcg atcatcgctg
      841 aattccaaag ggaacgagta aaattcagct cattggaaaa caaattttga aaattgatga
      901 tcaagttact gccaatcttt atttttcccg atgtgatatc cacgggtgta ttcgtcattc
      961 ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga taggatgctt aatgctgtat
     1021 actttaatac tgacccaatt gtcgaacgaa gttctaatgc ttgttttttg acgttggggc
     1081 gttcgattgt ttctttttct accatatttt tcatcgactt tctcctccta ctttcgtttg
     1141 gattatcgac aatatcacaa taaaggaaac aataacaaag gaaactgctg ccgcatatcc
     1201 aaaattaggc gtgaatttat aactcaaatt atagatgtat tgagataggg tggtcgttgc
     1261 atttgctggt cccccgccag taatattttg tacttcatca aaaagctgca atgtcccgat
     1321 cgtcgaagta atggaagtaa acaaaatgat tggttttaaa tttgggatcg tcactttgat
     1381 aaactgctgc catttagaag caccgtcaat agatgctgct tcataaatct ccgaatcaat
     1441 attttgcatg cctgaaagga aaaaaatcat attgtatcca gtccaacgcc aagtgatcga
     1501 aagaatgatc agaactctcg cccaaattgg atgagtcaac cagccaatcg gtgtgtcaat
     1561 cataccaatg cccaacaaca agttgtttac aaggccagat tgagaaaata gacttttcat
     1621 gatcagagaa taactgacca tcgaagtgat acatggaaga aagatagcgg ttcgaaacaa
     1681 ccctttatat ttcaactttt tatcatttaa aaggttggaa ataaaaagtg ctagaaacaa
     1741 catgatcggt acttgtacaa gtaaatacag gaatgtatta aaaaaagctt ttttaaaagt
     1801 tgcatctgtc aacatccgct gataattacc taacccgtta aatgtcatat tgacaggggg
     1861 accactttga aaagacgtga tcaaagccaa aaccatcgga ataaaaacca tgattgatat
     1921 caaaatgact ggcaagatta aaaaagcatg cccgtttttg aataaatctt tctgcttcat
     1981 tcttttccct ccaattctat cattgattat ctacatgaaa agacaagaaa gacaatatct
     2041 gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg atgaaaaagc gaagaaattc
     2101 acttttttac caccgatcaa ttagccaatt gtgcttctac ttgtttttgt gtatctgcta
     2161 gtaccttgtc tgtctcttct ccattgatga ttcgatgaag agcttcagct acgactgctt
     2221 caattgcata ggtctcatgt ccgtaattca cttctggtat ttcagatgtc catgttgaaa
     2281 aatcgtcaaa gatcttttga ccactgtaaa attcagaagc ttcttgataa gcttcttgtt
     2341 cattcgcgct aagcatcgta gatactagtc cgatttcttt cgcaagagtg cccatcagtt
     2401 cttcattcga agcaaacgtt gcttctagga aatctttcgc tgaatcttct ccagctactc
     2461 ctttgatcac ataccagcca gcgccgccaa ggttagaagc ttgcgctttt tgcagatttt
     2521 ctggaagtgc aggtattggt gcaatcttcc attttccaga ttggttttct gctccttgga
     2581 tcgttgaaga ataccaagcg ccagttgggc tagaagctac tgctcctgat tgtatcgcag
     2641 ttactccagc attccagtca gaggtttgtt cgactagatc ttctttcaaa agagtggcaa
     2701 acaattcaag accatacttt aaagatgcat tgtcttggat cgtgactgtc tccccatctt
     2761 cagatgtata ccattctcct gcctgttgca taatcatcct tactcgacca aggtcagaag
     2821 gattgacttc agcgattttc tttcccgtct tttctttgac atctcgcgct acttgaatat
     2881 actcgtccca cttcagattc tccatatcct cttctgtata acctgcttct gccatcagat
     2941 ccgtacgata gaagtttgct gttacgcccg aatcgaaagg aacaccgtat attttatctc
     3001 ctactttgtt taccgcaaat ttatacggtg caaaatgatc ttcctttaca atagcagaca
     3061 aattagcgaa tgcatctgga taagaagtca aatagccttg tatcctataa tcttcaatca
     3121 agacgatatt gggcaatcct tctgtattcc cgctcgctaa agcggtgttc agtttctgaa
     3181 cgatatcatc ctgagacatc gtaacaactt ctatctctgt ttcttcgttc tcataaactt
     3241 tttttgcttc attcactgct ttgatattga atgtctcatc ccacgcccag acagtgactt
     3301 tttcttcttt gtctgctgta ctactagcac ttccgcatcc tgttaagaaa cctgatgctg
     3361 ccgctaaaca aacgactcca gcaattaact ttctgatgtt catttttatc cgctcctttt
     3421 ccatccatta gttgtttacg tttgctacat tcatcatacc aaaatttgaa aacggtttct
     3481 ttattattca gatacttcaa aaatttctcc taaaataata aaaaaataca aatcaactat
     3541 gacaaaagga ataatcttag catctagacc aaagatcgaa atggtgaggt ttctttaaaa
     3601 taaaaatatc cttgcatttt ctcctagaaa taagtatttt taaattacaa gactattctt
     3661 aaggcgggta aaaaaatgcc aatacgcgta attcttatcg atgatgaaca gacgatttta
     3721 gatggaatga aacagttgat caattggtct gagcacgggt ttcaattaat agggactttt
     3781 tccagtccgc tgaaagcgct tgatttttct ttggtacatg caatcgatat cgtggtcaca
     3841 gatgtaatga tgcctgaatt aaatggaatc gaattatcgc ggctattaaa acagcaacaa
     3901 cctgaaacac agatccttat cctctctagc tatgatgaat ttgatatggt taaagattct
     3961 tttaaagaag gcgtcgctga ttatctttta aagcctcgat tgagtcctga tttttttcta
     4021 aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc ccaacaaaag cccaatcacc
     4081 aatcgttatg agcagatctg cgagaatctt tcacagcaga ttgctggaca aactcgtcca
     4141 ttgcttgacc ctcttctttt tgaacacacc ttgtttttct gttatatacc gaaaaacggc
     4201 cattcagttc aaaacaaggc tctaaacaaa aacagtcgat cacccattca ttgatggata
     4261 gcgacgattc gtttactatt tatcctgttt ctaccttttc ggaagaatcc ggctatgtaa
     4321 tcaatactga ttccaaagat acacttagga agtttgttca aaagtttcgt caagaagttt
     4381 ttccaggtga tttttttgtt gtttctgatt cttttgcaat ccaagatatc aatgaaatat
     4441 tccaagagat cagaaattgc accaaaggac aacgtttcta tcataaggga aagaaccttg
     4501 cttatcagca tgaacttctt ccgttattaa ccaaccacga gcagcaagca agtagttatt
     4561 taacaaaaat cgttcataaa gactacgctt tagctgtcaa agaacttgag aatttcttca
     4621 cgacgatatt ggaacagcct attgatccag cttatttgaa acatgaggca atcaatcgtt
     4681 tctatgcatt actcaacgct ttggctgacg agcatgttgg gaatgaagaa ctttctttgc
     4741 tcaagatcgc ccttccttct ttattagccg atgctgagta tttcgaagat ttttctattc
     4801 ttgtcaaaga aacacttgag aaactcaccc acttttctgc aaatgcaaaa aaagacaatt
     4861 tcgatat
//
LOCUS       DBNICI010000177         4841 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.181, whole genome shotgun sequence.
ACCESSION   DBNICI010000177 DBNICI010000000
VERSION     DBNICI010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.181"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..308
                     /locus_tag="AC1ZCC_002529"
     CDS             <1..308
                     /locus_tag="AC1ZCC_002529"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630746.1"
                     /translation="GKNAFTFDISQIEHSVYFMSVLLIYSCFEGVRYIIEYSGFVYVH
                     YTEKLLVSKKSEDKHGKLPWKSEICEKSLYIKGFFFFLYHSFKPFLAVLDHFSIIFP"
     gene            548..1210
                     /locus_tag="AC1ZCC_002530"
     CDS             548..1210
                     /locus_tag="AC1ZCC_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630747.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            1289..4516
                     /gene="fss3"
                     /locus_tag="AC1ZCC_002531"
     CDS             1289..4516
                     /gene="fss3"
                     /locus_tag="AC1ZCC_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HIQ1630748.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            4630..>4841
                     /locus_tag="AC1ZCC_002532"
     CDS             4630..>4841
                     /locus_tag="AC1ZCC_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF961 family protein"
                     /protein_id="HIQ1630749.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQP"
BASE COUNT         1739 a          830 c         1005 g         1267 t
ORIGIN      
        1 ggggcaaaaa tgcgtttacc tttgatatat ctcagattga acatagtgtt tattttatgt
       61 cggtattgtt gatttatagc tgttttgagg gtgttagata cataatagaa tatagtggtt
      121 ttgtttacgt gcattacacg gaaaagctgc tcgtatcaaa gaaatcagaa gataaacacg
      181 gaaaacttcc ttggaaatct gaaatttgtg aaaagtcctt gtatatcaag ggctttttct
      241 tttttctcta tcacagtttt aagccatttt tggctgtttt ggaccatttt tcaatcatat
      301 tcccttaaaa attgtcctag attgtccgac attgactatc agtgtcgtca aaatagggca
      361 aaatttagta gtcagcttga cgacaaatat aatacgaaaa ataccaaaac atttaccgaa
      421 tttagcaact tttgaaccca agtttctcaa agggtcagct attagcaaaa aagacaaaac
      481 atgtactttt ctactaaaaa gttgatgtga taggcttcta tctaaataac tattaggagt
      541 ttttatcgtg aagcaaaatg atggacgtat catttggaaa aatcaaagtg agttaaaact
      601 gattttaaca attaatgaat tcatcgaaaa acacggaatt acatctagcc gtcaatacca
      661 gaaaaagcta tcagaaaatc caaattctgc accaagtatg tggttcatta acaaaaaata
      721 tggatcatgg gagaatttat tgattagtat aggacgagaa aatactgggt atggaaaatg
      781 ggctagaatg tcagaacaag aattattgga aatagtagaa gcctttatta agtgcgagaa
      841 gataacctct caacgaatgt acgaacaaaa atcagtaggg aagaatattc cttcacttag
      901 taccattaaa aagatgttag gagatatacg tccacttttt aaagaaaaaa atgatggatc
      961 tcgatttact gatttcgaac tgctgttaga actaaaaaat gaaataatcc gattaaaact
     1021 acaggatgat ttatcaatga caaagtttcg aaaactagtt caatccccaa aactaccatc
     1081 agttgataca attatgaaaa gaacgaacaa aaattgggag gaattgatgg cagagattgg
     1141 atttgattat cgaagaatca aaatttataa gcagagaaac aacctatcca agacaaagaa
     1201 aactaaatag tatcactcta aatcagacgt tcaaaattaa aatttggacg tcttttttat
     1261 tacccaaaaa cgaaaggaga tttattttat gaaaaagcca agatttaaga attggcgttt
     1321 gctcgcaaca ctgagtctat tatgccaaac aattggtgga tcgcttggcc caacgattgc
     1381 ctttgctgat gaaattactc atccacaaac ggtaactgtg gaattggatt tggctcacca
     1441 gtatgctgtc gaaggtacat ttagcgatgg tcgtcccatg tcagaagtta ccgttccgca
     1501 ctatgcagtt tacaatggtg tgaagcaaga tattttctgt attgagccag gggtgccgat
     1561 ttacaatgag ttcactcctg gttatgagaa aaatccattg cctgatatgt cggagaaagc
     1621 gaaattagtt tccgttttat ggaaggaagc tggtacggat gtggatacac atattgtggc
     1681 ccagaagatg atttggcaag aagttaatgg ctacacgctt cactcaatta aacgttctga
     1741 tggtagtgca gtaaatatcg cagccattga gtcaaaaatc aatcaagcca ttgcggatta
     1801 tcaaaagaaa ccaagcttcc ataactccac agtgaaaacc gtgttaggtc aatcgactac
     1861 tgtgacagat acgaacggct tgaatttatc ggagtttgat gaagttgtcg agaatatcgc
     1921 aaatattgat tatcgtgtga acggcaatca actagtgatt actccaaatg ccaattcaaa
     1981 agagagtggt gaattaaccc taaagaagtc agctaataca ggaacaccgg tagcttataa
     2041 aatggctggt caacaaactt tgatggcggg ggcaattgat aagcccaata cctacacaat
     2101 caaaattgat gtggaaactg agggttcttt gaagattaaa aaagtcgata aagaaacagg
     2161 aaatgttgta ccgggaaccg ttttccattt agactttgga aaaacattac ctgcaaaaga
     2221 cgtgaccacc gataaagaag gcattgcgac attggatgag attcctcatg gcgcgaaagt
     2281 aaccattact gaaaaatcag tgccagctcc ttatacgatt gacactacac caatcaccgc
     2341 aaccattaaa tctggtgaga ctatttctgt gacttcaaaa aatactcgcg aaaaaggtca
     2401 aatcattttg aataaaatgg gggtggaaac aggaactgat ctttggaacg acaaatactc
     2461 attagccgga aatacgtttg ccattcgcaa agacagtcca accggtgaaa ttgtccaaga
     2521 aatgaccacg gatgaaaaag gtcatgcgga aacaccaaaa gagattgcca atgcgttgga
     2581 attgggaacc tattatgtga ccgaaaccaa agctagtaat ggctttgtga atacctttaa
     2641 accagtaaaa gtcgaattga actatgtcaa tcaaaccgtg gctcttgtta ccagtaacgt
     2701 aaaagggcaa aaccaagaaa tcactggtga aaccactttg acaaaagaag acaaagatac
     2761 cggtaatgaa actcaaggta aagctgagtt taaaggagct gaatatactc tctttactgc
     2821 aaaagatggt caagcggtca aatggagtga ggcttttaaa ccagagttag tgaagggaac
     2881 gaaagcttct gatgaaacgg tgactttgac tttagatgaa aagaatcaag ttgctgttaa
     2941 acacttagcg attaatgagt attactggca agaaaccaaa gcacctgaag gatatacctt
     3001 ggatgaaaca aagtaccctg tttccatcaa aaaggtggat gataacgaaa aaaatgccgt
     3061 gattacccga gatgttacgg caaaagaaca agtcattcgc tttggctttg atttctttaa
     3121 atttgctgga tcagctgctg ggactgccga aactggcttc aatgatctga cctttaaagt
     3181 atcgccattg gaaggaacca gtgagattac aggtgccgaa gatgaagcaa ttacagctta
     3241 taacgagcaa ctaggttttg atggctatgg caagtttgaa aatcttcctt atggggatta
     3301 tttacttgaa gaagtagagg ctccagaagg atttcaaaaa attacgccat tagaaatccg
     3361 ttctactttt aaagaaaata aagcggatta cactaagagt gaatacattt ttacgattac
     3421 cgaaaaagga caaaaacaac caattaaaac ggtgaccgtt ccttacgaga aactgacgaa
     3481 taaagcattt tctgttagct tgaatcgttt gatgctctat gatttacctg aagaagaaga
     3541 tagcttaact tctcttggga cttggaaaga cggaggcaaa gaattgacag accttgattc
     3601 taccgagcta gttgataaat tgagctataa cttgcatgaa atcaaagaag actggtatgt
     3661 ggtagcgcaa gccattgatg tggaagcgac aaaagctgcc caagaaaaag atgaaaaagc
     3721 caaaccagtg gtaattgccg aaacaaccgc aacgttggcg aataaagaga aaacaggaac
     3781 ttggaaaatt ttgcataaat taaccgcaga acaagttttg gataaaacca tcgtcttatt
     3841 caactatgtg tatgaaaaca agaaacccta tgaagcaggt gatgatccag tagcgaaaga
     3901 tgctagcttg aacaatcaag cccaaactgt caattgtacg gtggaacgcc atgtttccat
     3961 ccaaacaaaa gcccacctag aagatggttc gcaaaccttt actcatgggg atgtggtgga
     4021 tatgttcgat gatgtgtcga ttacccatga tgtactggat ggctcaaaag aagcttttga
     4081 aacaattctg tatgcattac ttccggataa tacgaccaaa gaaatttgga aatctggcaa
     4141 aattgattat gaagtgaatg acaaagaatt cactaaaacc gtacttgcgg aaaaagtgga
     4201 cacaagtaaa tatccagaag gtacaagttt taccttcaaa gaaattaact atggcaaaga
     4261 cggaaacatc aatgggaaac acaatgagga tctaaaagaa aaaactcaaa ctttaacacc
     4321 taaaaaagtt ccgacaacac caagtgcacc agaacaaccg gaaataccaa ctgttccaag
     4381 tgactctcaa gaatctagtc ctacagtaaa gacattccca caaacagggg agaaaaattc
     4441 taatgttctg ctgtttattg ggtttacctt gatctttgcg actgctgggt attatttctg
     4501 gaatcgccga aactaaggtg atgtgatgaa acaactaaat aggaaaaacc aacgtgcgcg
     4561 accgcctcct aaaaaaagtg gtcgcgctat ttttgtatcc aaaactaaaa atgaaaagag
     4621 gaaaagaaga tggaattgaa atttgttgtg ccagatatgg ccgaaacctt tggaaagata
     4681 aggtatgccg gcgaaggcga agttttaact gaaggatatg ggcggaatac tacagtaatt
     4741 ggtcgtagtt atcatttgta ttccagtaag caacgagccg atgatattga agtggtggta
     4801 gctgcggaag ccggcgaaaa ggattttgat caagatcaac c
//
LOCUS       DBNICI010000178         4810 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.182, whole genome shotgun sequence.
ACCESSION   DBNICI010000178 DBNICI010000000
VERSION     DBNICI010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.182"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(92..1177)
                     /gene="xerS"
                     /locus_tag="AC1ZCC_002533"
     CDS             complement(92..1177)
                     /gene="xerS"
                     /locus_tag="AC1ZCC_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HIQ1630750.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            1526..1721
                     /locus_tag="AC1ZCC_002534"
                     /pseudo
     CDS             1526..1721
                     /locus_tag="AC1ZCC_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
     gene            complement(3253..3771)
                     /locus_tag="AC1ZCC_002535"
     CDS             complement(3253..3771)
                     /locus_tag="AC1ZCC_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HIQ1630751.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            4138..4386
                     /locus_tag="AC1ZCC_002536"
     CDS             4138..4386
                     /locus_tag="AC1ZCC_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630752.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         1581 a          786 c          729 g         1714 t
ORIGIN      
        1 atataaaaat gataatgaag cttgccttta aagttgggct tttatctaat tttagagaca
       61 ggcttcatta ttatactatt ttttactaca tttacaaagc gttcaaagca tcaattgtag
      121 ctgcattgtc tatatgagta tataaagttg ttgtatttgt acttgtatgt cccaattgtt
      181 gggcagttaa taccaatgaa tttgtctgtt gatagagtct agtagccaaa gtatgacgta
      241 atttatgtgg actgacttgg actctgaatc cttttgaata ttttgctacc atccgttcga
      301 ttgcaatacc ggatatacgc ttggcttctc cttgataaat tgctagaaat aaagcttttt
      361 cgttttctgt tactttgtat cgttctttcc gaatagctag ataattagct aaatattcca
      421 ttgcaaatgc tgctatattt actgcgtctc tcttatttcc ttttctagtt actaccacat
      481 tattcttttc taaagatagg tcctctaaat taatatttac aacctcggag agtcttaatc
      541 cagatcctaa aatcaatgca ttgatcgcca ggtctctttc tacatctcgt tgataataga
      601 tcaaagctcg tttgctcaaa gtttttttat attcattggc aatataatct agataatttt
      661 gtgtatcttc gtctagaaat aattttttct cgattgcgtt tgcacgggca gctaacgtag
      721 ttttatctgt gatagttgct acttgatcca taggattatt ttctaagtat ggttttccta
      781 aaggattatt ggaacttttc gaaagatatt taaatagaac ctttaatgct gtgatatttc
      841 tattgatagt agaagtagat agacctaagt tattattatc tttgagctta gtttttgttg
      901 tctctttttt tacattttcc tttgtgcgac gtttcaaatg agatttgtac aagtttagat
      961 cataggcagt taactgtctt atcgaggtga ttgaagtatc gggaacgata gactcactca
     1021 gaagccattc ataaaagagc ttataatttt ttagatattc taaagtagtt cgaggtgatt
     1081 gatctttatc ctcacgatct tgaacaaaat cttttatata atcgggcata tgaagtatga
     1141 ttgtatctat tttttgatat atttctgtat ttttcatgtt attcatcctt tcgataagta
     1201 ggggagagga gtatctctat aattggacta taccaattat agagtgtttt gtcaattggt
     1261 atactctgaa atttattaat ttatttcaaa aaagcagata aataggtata gatttaataa
     1321 tttaaaataa atggtataca tgaataggaa aggaaacaga aggaggcaat cttaattttt
     1381 taaattgtct aaattatata gaaagaacaa tctttctata taattagaca taagagagac
     1441 tacggttgat ttgatattaa caagcgtata tgaatgtata tacaaataaa aaaggggagg
     1501 gagaagttaa agaaaattca gagaagtggg gcactcagta gtattaacaa tttaaaagga
     1561 atttcttaaa gaaaatgggt tgcatcctgg agatacaatt tttttagata aagaaaaaat
     1621 tcaagcaaca attcaaaaaa atcttaactg ctgagattga tgtagcaata tctcaatcat
     1681 caacaaagta cgatcctgcc tttcaaaagc ttattgataa atgtttattc attataaaaa
     1741 taaaagaaaa atttatgtca gcttttttca tcaaagtggt aatgataatt cgtctattaa
     1801 ttgtattttt taggaaatat ggtataattg ttttaacgaa aaggtaattc atgatacacc
     1861 aaaaaccact agctggcggg ctagtggttt tcttttacgt tctattcaga ttaacgggta
     1921 ggcttactgt cttttttggc aataattttg caatcaagcc atttacagat gtaatgcgcg
     1981 gatacttctg ctaagattgc aaaaaacata tcgaaaaggt aattcataat acaacctcct
     2041 ccccttgcca taataggccg gggtagcaga cagcataaat attataccat aactgaaaat
     2101 ttcattttag tacatttcaa tatattgatg aaccagcaca ataaaacatg ccatgactaa
     2161 cagaaaaaaa cttattaaac cagatataac ttgtaaacat ccaactatat agaaaacgta
     2221 aattttctat atagttggat taagataacg actatttatt ttatcatatt tttactatac
     2281 cctatacttt tacttggtat atttatgaat aatcgcaaat ttacccctat actactagtt
     2341 tgtaagttag attaataaaa atactaaagt tatttgttct tttattaaat acacctacaa
     2401 cttataaatt gatggtattt agataacacg aaagaataaa aataacagct gatagtaaag
     2461 tttgttgacg attgctactc aatcgaaagt gagttagtgc gtatgaactt gcttctgaaa
     2521 acttatgaaa tgaaagacct tttacatctt gcagaaatcg ttactgggat taaactaagc
     2581 aataaaaact aatgttcccc tcattctttg aacttctact atttgattta tttattctga
     2641 taaatgacga tttcaccttc aggatcttca aatcccgtat agaatttatt taagatctct
     2701 tcagtatcag taataggata ggcgataggc cacataccct ttgatggtta gaataaactg
     2761 ctcaaaatct gtatcgtatg ttggctttag catattaaca aaggctatct ccaaattata
     2821 aaccataagc gttgaatcgt tcttaacaat ttcaattctt cctaacccaa agtcagaaga
     2881 tataattacc ggacttgcct tacccttcat gtgggttgtt gagattccat atttaaaggg
     2941 aattgtcggt actatcggcg gtctgaaaaa catcccaaaa ataaaaaaat ctttcccctg
     3001 ctagtctcct aagtcccttt gtagcggata tggcaccata atctcatttt atcgaagtga
     3061 gtcatggttt atctgttggc caaccaaaat tgatacaatc taacgatgca ccctctacgg
     3121 cattctaggg gagtgcactt gcattcaccc aataaaaacc ggcagttagc aaagaactta
     3181 tgtagtcgta tgttcttact gttaacatac aagaggtctc ctttccacaa ttgcttagca
     3241 gtaaacggga atttacaaag ctaatctctt ccattttctt atctttgttc taaaagtccc
     3301 aaatggagca actgtattta catgaataaa tttatatact tcccatacag ccgttttggt
     3361 tgcctcgtct gcccattttc ttttgtgtgc agtgaataga tcttcatcac tcattgaatc
     3421 aatcatcatg ttaatatcat caatattacg attgagaatt tcctctaatt ctttcaatgg
     3481 tagatgtgca taggtatttg taaaccactt atataactct cctaactgat tccatttaaa
     3541 ttgatcagaa ggcgttttaa cagataatcc atttctttca tcattttccc attttagaat
     3601 taaattcgtc caaccaactt gatatgctaa attttcagca ggtgttctat ccacttcctc
     3661 aactctttta tccttcaatt cttcaggaat agtttcaaat tctaaaatat atttagtata
     3721 agtcctattt atttcttttt ttaattcttc tttattttta tatgttctca ttatattacc
     3781 tccataaatt cctaattgtc tcctttaatt catctataat ttcatccacg atattttaat
     3841 ccgttctaca atgcatatct gatatgcatt gactgtctaa ctcatatgtg aatgatttat
     3901 aaaatatccc aacgtctcat ctaaagaaat gttgatcgtc agcaaggagc taaaaaatac
     3961 gaatatattt cttttgtagt aaaatcggac tggtttgcat atcattttct tcgtaaatat
     4021 caaaatcatg ttaactacta tcaattacta ataaaaacta ccactttaag cgttaagaac
     4081 tgttaagttt agttaagtga ggatagttca caaaatccat tgttaaactt cacgaaagtg
     4141 gtaaaactca aaatgcttta tataggatta cagcatcgct ctttcttcga ttgcgcgttg
     4201 ggtaaaaaat attatgaagt aaaaattgac gatgacacgt tccttacggc tcatcagatg
     4261 aaacagctac aaaaacgaaa tgatcaactc gaagaaaatt taattctaaa aaagcgattg
     4321 ccatattcac gcctcgctcg acgaactatt aaaggtcaag atttacctga agatcatcct
     4381 ttttaggaaa ttgaagcttt ttatgttgca caatgcattt ataatactac aatgatgttt
     4441 tcaccagata ttcttctagg gtgcggtgta atgaaacaga tacacttgca aaagaaaatt
     4501 caccgcgaat ttactaaaat tatgaataat tatgttaatc ttccgaaatt tttttccaaa
     4561 attaagggat caagctggtg ttatagattg tttaaggttg gcaaaaaaat aacttctttt
     4621 taaacaatta tagaaaaccg atactctgtt aagttggttg acacggtgtc gatttttatg
     4681 actttcttat tctgaaactt cactaacgga cagtacctaa aaagtctata gtatggacgt
     4741 ttacgagtac tattttatca aaaatgctgc tcatttcaac ttatatgttt tagtgaacta
     4801 cccccactta
//
LOCUS       DBNICI010000179         4803 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.183, whole genome shotgun sequence.
ACCESSION   DBNICI010000179 DBNICI010000000
VERSION     DBNICI010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.183"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            159..2006
                     /locus_tag="AC1ZCC_002537"
     CDS             159..2006
                     /locus_tag="AC1ZCC_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIQ1630753.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2009..2905
                     /locus_tag="AC1ZCC_002538"
     CDS             2009..2905
                     /locus_tag="AC1ZCC_002538"
                     /inference="COORDINATES: protein motif:HMM:NF024700.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIQ1630754.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            2955..3344
                     /locus_tag="AC1ZCC_002539"
     CDS             2955..3344
                     /locus_tag="AC1ZCC_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HIQ1630755.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3331..>4803
                     /locus_tag="AC1ZCC_002540"
     CDS             3331..>4803
                     /locus_tag="AC1ZCC_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIQ1630756.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITSRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFG"
BASE COUNT         1690 a          696 c          984 g         1433 t
ORIGIN      
        1 gagacttgga gattggcggg aattatttgg ttacgagtcg aggggttttt gaactaatgt
       61 aaatcatttg ttagaattga ccagaaattt ctatcgtact tttacttttc tatttgatat
      121 actgaaatag aaaatatttg ttgatagggg tgttactaat ggttggcaat aagtggtaca
      181 aatgtgatct tcatattcat tcaactgcga gtcagtgctt tcaggataaa aaggttactg
      241 ctaaggaatg ggtagaagaa tgtttaaaca aaggactcga ttgtgttgca ttaactgatc
      301 acaatacagg ggaaaatatt gatgaatata agaaagtggc tatcgaaaat ggcttagatt
      361 tttttcctgg agtggaatta acgtgtggag aaaatggaac tcatttacta gtcctatttg
      421 ataaaagtgc atgtacaaat gatatctctg atttcttaac caagatgggt ataaaaagag
      481 aggattgggc cagcgctgca gcttgctcaa tgaaatcagt tttagaagtt attgattttg
      541 ctgagcacga gggcttgctc gttattcctg cacatataga tgagtttaat ggaatttctt
      601 cagcaaaatt tggaaatcta ggaaagatat ttgaatcaga aaatatatat gcagtacaga
      661 cggttcaaaa agaatttttt gaaaatcgct cacaaatgat tccctcatct aagcgtgaca
      721 ctatctatga tagtgtgaat gccagatatg atggtagagt tggaaaagat actttggaat
      781 cttggtataa atcagttgtt gaggcagaaa aaaataatat gacatttctt agcttttctg
      841 ataacccaca ctcgaaagga aattcaaaac atggactttg gggaataggc acaagatatt
      901 cctatataaa aatgaaggac gaacctgact tgggatcact tcgcgatgct ttaatgctag
      961 gcgaaactcg agttcattct gatttttcta atttctccat taatgaaaat gaagttttat
     1021 tggaaaaatt aagcttttct gggaccactc ttagcacaaa agaagttgta gtcgaattca
     1081 gcgataattt aacttcaatt attggaggaa gaggaacagg caaatcttgt attacaagat
     1141 tcttggtcta tgtccttgga aaggaagcag aacttgatca attttctgaa atacaaagtg
     1201 attatcagaa ctttgcacaa attgaacata atggctctgg aatatttttg aaggatacaa
     1261 ttgtgaaact gaatattttc tataagggga caaaatatca aataataaga acacaggata
     1321 gacattctat ttatgaattt acacaagaga atggtctggt tgaaaaagaa acagaacggt
     1381 tgagaatgat ttccgataag gtgagtatat actctcaaaa acaaatttat gaaatttcaa
     1441 aaaatcaaac tagtatacta gaattactag atggatataa ctctgatttg atttcagagt
     1501 ataaaaatga gattgaaaca tgtgtaaacg aaataagaaa attaaattgg gatattgttt
     1561 ctgtgaaaaa agaaatacaa gataaggcga aggtagagct tgaaattgaa gatttaaaaa
     1621 aacaagtaga aaagctatct cataagagtt ataaagatgt ttatgatgag tattcaaaag
     1681 agacagatat ttatagggaa ctgaaacgtg atgtggaggc acttaaggaa attcccaaaa
     1741 atctaactga tagtgttgac aaaattgact ttggcaatgg tataaaagtt acagacgaaa
     1801 ttgatgaaca ccgcggagaa ttgattaaga atttagtgac caatcgggct aagattcaaa
     1861 caataattaa tgaaatgtca gatgaaattg atagttatag agcagagctg aataaatcag
     1921 attggaaagt taactataat gaggtttcaa agaggtatca aagaggtatc aaaaacttag
     1981 tgaagagtta tctcaagatg aattaaaaat gttacaaaac cttgattctt tatatcaatc
     2041 tttatcaaaa actgaagagc gattggaaga aattattcaa caagaaataa agcttaaaga
     2101 ttaccaagtt gaggttgact cagcaatgtc aagaatgaat aaatcatatc aaaatctcta
     2161 ttctgagaga agtagttcct cgcgcgaaat tttttctgat attacaggaa tagaggctac
     2221 tattcaacca aatagggatt tcaataatta tgttaaagag ctcagagctt gtattaataa
     2281 ggaggacact tttgacagtg agtttggatc tcttattgat aagctgacaa aaaattccat
     2341 tactaatcaa gatatatata atgacatact tgatataaga gttagtgggg aaagtagtat
     2401 tttttcagat aaaaaattaa tcaattctat aagaaaattg aatgatgcac aagttcttga
     2461 attacaaacc cttaagccaa atgatttagt agttatccga cttgaactaa atggaaaaaa
     2521 agttagttta tcgaatgcta gtgctggtca aaagacatct gcaattcttt ctatgatttt
     2581 ggcgtatgga aattcaccac tcatattgga tcagcctgaa gatgatttgg atagtcaatt
     2641 aattaataac ttaatcgtaa gaagtatcat tacaaaaaag gaaaacagac aaattttaat
     2701 tgtaactcat aatgcaaata tcccagttaa tggtgattca gaatggttga ttagcatggg
     2761 agattcaaaa gaaatatcgg ctgatcagtg cgggtcggta gatgaaccca atatcaagcc
     2821 aagaatatgt actgtgatgg aaggtggcga ggatgctttt acaaatagag cgaaacgcta
     2881 tggttttaaa caaattgtag aataacacta gagggtgaga catattatta tgtccgccct
     2941 ttttaggagg aaaaatgaaa aaaataaaaa gctataccag tatctgggca gttgaaaaag
     3001 tcatttacgc aatcaatgat tttcagttgc cttttcctgt gacttttaac caaatggctt
     3061 ggttcgtttt atctttactg tttgtcatcg tatttgccca tgtgcctcca ctatctatga
     3121 tagaggaggt ctttttaaaa tacttaggca taccggtggc tgtcacttgg tttatgtcgc
     3181 aaaagacgtt tgatggcaag aaaccactgg gatttttacg atcctttatc agctatcact
     3241 tgcgatttaa ggtgactttt gcaggaaaga aagtcaaaga acagaagaaa cgatgggatg
     3301 aacccagtac tcttgtaagg agtgtgaact atgtatccga ttaagtatat tgaaaataat
     3361 ctcgtcttta accaagaagg cgagtgtttt gcctactatg aattagtgcc ttacaattac
     3421 tcctttttgt cacccgaaca gaaatttcaa gtgcatgata actttcgcca actaattgcg
     3481 caaaatcgtg aaggcaagat ccatgcgtta caaattgcca cagaaagtag tattcgcgcc
     3541 acacaagaac gatcaaaaaa agaaattacc agtagactca aagaagtagc gaaacaacga
     3601 attgatatac agacagatgc attagtatcc atgattggcg atagtcagat tgattatcgc
     3661 ttttttattg gctttaaact aattgcgaca gatgaagaag tcaatctaaa gagtctcaag
     3721 aaatctttct tttctgggct tcaagaattt gtctatggag tgaatcatca tttgatgggc
     3781 gattttgttt ccttatccaa tgaagaaatt cgctgctatt ctaagcttga aaaattaatg
     3841 gaaagcaaat tggctcgaag atttaaagtc aggcgagtca caccgagtga cctgacgtat
     3901 ttgattgaac atatttatgg cgaaaaaggg ataccgtttg aagaatacga atttcagcta
     3961 ccaaagaaaa agctaaaatc agaaacactg gtgaaacgtt atgacttatt acgtccaaat
     4021 cgttgtttaa ttgaagaaaa accaaggtgc ttacggatgg aacatgagaa tcatgagtcg
     4081 tacgtagcgt atttaacaat taatacaatt gtgggggaga tggaatttcc ttcctcagaa
     4141 cttttctatt accagcaaca acaatttaca ttcccaatcg acacaagtat gaatgtagag
     4201 attgtgacga ataaaaaggc tttagctacc gttcgtaata agaaaaaaga actgaaagat
     4261 ttagacaatc atgcttacca atctgacaat gaaacgaatt ccaatgtctt agatgctttg
     4321 gattccgtgg atgaattgga aaccaccttg gatcagtcaa aagaatctat gtacaaactg
     4381 agttatgtgg ttcgagtgag tgccgaatca gtggatgaac taaagcgacg ctgtgatgaa
     4441 gtgctcgatt tttatgatga taccaatgta aaactagtcc ggccctttgg cgatatgatg
     4501 gggctacatg aagaattctt accctcaagt aaacgatata tgaacgacta tattcagtat
     4561 gttacgtctg attttttggc ggggcttggt tttggggcta ctcagatgct aggagaatta
     4621 gaagggattt actttggcta caatgtggat acaggtcgta atgtttacct gaaaccggca
     4681 ttggcttcac aaggagtcaa gggttcagta accaatgcgc tggccgccgc tttcctgggt
     4741 tctcttggtg gaggtaaatc ctttagtaac aatctcttag tctactatgc tgttttgttc
     4801 ggt
//
LOCUS       DBNICI010000180         4685 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.184, whole genome shotgun sequence.
ACCESSION   DBNICI010000180 DBNICI010000000
VERSION     DBNICI010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.184"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            394..1527
                     /locus_tag="AC1ZCC_002541"
     CDS             394..1527
                     /locus_tag="AC1ZCC_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIQ1630757.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            1558..1878
                     /locus_tag="AC1ZCC_002542"
     CDS             1558..1878
                     /locus_tag="AC1ZCC_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HIQ1630758.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(1907..2371)
                     /locus_tag="AC1ZCC_002543"
     CDS             complement(1907..2371)
                     /locus_tag="AC1ZCC_002543"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1630759.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(2502..3759)
                     /locus_tag="AC1ZCC_002544"
                     /pseudo
     CDS             complement(2502..3759)
                     /locus_tag="AC1ZCC_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(3788..4105)
                     /locus_tag="AC1ZCC_002545"
     CDS             complement(3788..4105)
                     /locus_tag="AC1ZCC_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HIQ1630760.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(4131..4451)
                     /locus_tag="AC1ZCC_002546"
     CDS             complement(4131..4451)
                     /locus_tag="AC1ZCC_002546"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIQ1630761.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
BASE COUNT         1491 a          945 c          839 g         1410 t
ORIGIN      
        1 tgaaaaaggc gtttagtttc gagtatgaag aaaaattttg tgaaaatggt tcctcacgtt
       61 tttgcataaa tttgacttaa taccgagaaa ctgatccctt ctgtcataac ctctttttat
      121 ttatgtctgt ttctttttga aaacaactat tttttcaacg tttactctct tgccaatcca
      181 acatcatcta acggatacaa ctatttcaat gattaccatt acactaaaaa atcggaaaca
      241 cacctctccg aacacaaata cataagacta tagaatgttt catttaagaa aaaaagcgtt
      301 tcattatcaa aaaggtaaga tctcctttaa aatgaaatta tctgtagtac taataatgca
      361 gacaatttta tgaatctgaa aggagcatct ccaatgaaag cagtcacttg gcaaggaaaa
      421 cagaaaatgg aaatcagaaa cgtagatgat ccaaaaatat tagaaccaac agatgcaatc
      481 atccgtatca cagcaacagc catttgcggc tccgatctgc atctttacca tcatggtgaa
      541 gcagttttgg aaaaagggta tgttgtagga cacgaaccaa tgggaatcgt tgaagaagtg
      601 ggatctcaag ttcaaacatt gaaaaaaggg caacgtgtgg ttatcccttt taatattagt
      661 tgtggtcatt gtcatttctg cctgaatcac atggaaagcc aatgtgatga atcaaatcca
      721 gatcagttat atggcgggct tttcggattt ggcaagttga acggtaatca ttggggtggt
      781 caagcggaat atctaagagt tccttatgca gactctactt catttgttgt accagataat
      841 gatcttcctg atgaaaaagt tttatttcta tcagatattt tgcccacagc ttattggagc
      901 gttgaaaacg caggagtaaa aaaaggcgat acagttgtga ttctaggttc tggtccagtc
      961 ggtctattcg ctcaaaaatt cgctgttatg gctggagcag agcgcgtaat cgcagtagat
     1021 ccagtacagc accgtctaga taaagcggct aattacaatg gggttgaaac ttatttctta
     1081 gaagacacgg cacaagctgg aacagaattg tatgaactgg ctaaaggtgg cgcagatgtc
     1141 attatcgatt gtgtaggaat ggacggatta gagcctgtca aagaaaaagc gaaaaattta
     1201 gtaagcttac aaagcggcac aatctctcca ttacagatgg catcgcaagc tgtcaaaaaa
     1261 tttggaacag tccaagtaac tggtgtttat atgactccag cttcttctta cccgctgcaa
     1321 gaatttttta tgcgggatat cgctgtcaag catggacagg caccaattat ccatctaatg
     1381 ccaaaaattt acgcaatgat tgcagaagga ctattcgatc ctacacaaat cattactcat
     1441 tcgctgccac tatcagaagc agcacaagca tatgagatct ttgacaaaaa agaagataaa
     1501 aatataaaag ttgttttgaa accttgaatt cagtaaaaaa tacaggtgat tttctttatg
     1561 cccaaaatca aacgaaatac ccatgagatc gaaataggaa aagaagaaga tatcgaaata
     1621 cgaggacgtc gcttccgctt ctatttccaa aatcgttaca cattgatctc actggctgtc
     1681 gatttattaa ccgggatttt ctatattttt ggaagccttg cttccttaac taaaatcccc
     1741 gatcgatatg gcacctattt ttatttggct ggtgctattt tcttgacgat tcgtcctatt
     1801 ctcagaatcg taaggaacat ttttatttac gacgaaaaaa aagcaatgaa gagaacaagc
     1861 aaaaaaaaag aggcatgaga atttttcaaa tctcatgcct cttttttcac cctatcgtat
     1921 cactttgata cagacgagcg gtcgtcccac tttcttcctt gaccattgtc aaaaatgtcc
     1981 actcgtatcg ttcataaaac gtggtatgat ctgtaattaa gtaaagtttt tcgtacccaa
     2041 gttgatgcat ttcttttctg atttccgcta atatccgctt ggctatcccc tgcttacgct
     2101 tttccggttc aacaaacagg gcacaaagat ttggtgtcag atcagggcgg ttatggaagt
     2161 cgttttcaat gacgcccgct cctccgacga tggtttcttt ttcatctaag accacatacc
     2221 attgaggaat actttcctta ttagcgattc cagtacgaat actttcctca tacacaacag
     2281 caggaactcc ccattttgat gaaaaccaat tcgcgctttg ttttactaat tctgggtgtt
     2341 ctcttaattt gatccagtta tatgtaacca atgtttaaac ctccgactaa aataaaacgt
     2401 ttacaaaaat tatacaagtc tttatgaaaa ttagcaatga caacaatagt gatgtaaaaa
     2461 aaaaacggaa aacaatcact tgttctccgt tttttgatta atcaagcatc taaagcttct
     2521 tttcttcttt cttggatcgt agctaccttg acgaacggta gatagatcaa aatagaaaga
     2581 accaaattga cagcggctag tacaccacct gcaatacttt ttgttgccaa tacaccgcca
     2641 ataattggtg gagttaccca tggaggcata acggtagctg ctggcactaa acctgtagaa
     2701 gtcgcaaagt aagcaacact aactaagacc attggtgtca aaataaatgg aatgaacatg
     2761 atcgggttca acacgatagg taacccaaac atcgttggtt cattaatatt gaagacacct
     2821 ggcgcaatcg acaagtttgt tacaaccata tatggtttgt ttttacgtcc tactaaataa
     2881 atagctaaca gtaaacctaa agttgcacct gttcctccta agttgacaaa gctgtcaaag
     2941 aaaggtttgt tcacgataaa cggtgcgcta tgcccagctg cgattgcatt aacgtttgct
     3001 tcaatggctg gcgcattgat tgtttgcatc aaaggatcga ccatattcgc tccgtgtaaa
     3061 ccaaagaacc ataagaatgg tgtgataaat gcaagcagta aggcagatgg ataagagttt
     3121 gcaagtccca taaatggttc ttggaccact gtatagaaag aacctacgat atcatcaaca
     3181 ccgaatgcag caaaaattgc agcaactaaa gcaaacgcag agatagtgat cattgctggt
     3241 agtaatgaag caaatgcttt tgcaactgcc ggtggcacac catcaggcat tttgatgatc
     3301 aatttttcat ttccgcttaa acgtacaaag atttctgttg aaataatccc aacaataata
     3361 gcaataaaca aaccgtttga gctcatacct aatgctccgc ctaaagcgaa gaaagagcct
     3421 aaagaaatca cacctgcgga aacaccgtct tttcggtagc cgttagctaa atgataagca
     3481 agcaagcaag aaagcaatca agacagaaag aatggcaaat gtaccgttcc aagcagcagc
     3541 cccgaatcct ttccatgatt caccaccaaa aattgagttc atcaattctt ggaacccggg
     3601 aatcggtaag ttattgatca ttactgctaa tgctcctaag atcattaacg gcatactcac
     3661 cataaatgag tcccgaatcg caactaaatg ccgttgtgca ccaatttttg atgctacagg
     3721 tataaaatgt ttctccataa atgcaacaaa tttatccata aatcttccct cttttcttta
     3781 ctgtagatta cgcgttcaat cgacgataca gatcaatgat ctcttttgct aaatctgtaa
     3841 aagctataga tgtcatcaga tgatcttgac tgtggaccgt caaaagcgtg acttcaacgt
     3901 gatttccttg tgcttcttgt gtcagcatct ctgtttgaga gtgatgtgct tggactaaag
     3961 attcctctgc atctttgatt ttttcatctg ctaaagtgaa atcacctttt ttcgcagcag
     4021 caatcgcttc cattgcatcg ctttttgcat ttccgccaaa catgatcaga cccatgatgg
     4081 cttctagatt tttttgatct tccatttatt gtatcctccg ataattttta ttatttgttt
     4141 attagttttt ctgcttggtc gagtactttt tcaccattca tcatgccata gtcagccata
     4201 ttgattacat ctaaaggaat gcctttaggt gctagctttt cagcaaattg attttgcata
     4261 aagcgtactt gcggtcctaa aagtaaaacg tctacatttt tagattctag ttgattatcc
     4321 gcatctgaag cagatacagc aaagatgtct gcttccattc cttttgcttc tgctgccttt
     4381 tgcatttttg tgactaggag acttgtactc attccagctg aacatacaag catgattgtt
     4441 ttcttttcca ttttaaacac gttcctttct atacttacac ttgtatataa gcaagtagcg
     4501 tgccaacttt aaaagcgctt taaaaagttg atatcatcac atttaaggtc tataccaagt
     4561 tgcatacaca ctaaaaaaga aagcgcacac taaacacaca aattaatttt tattaccttt
     4621 tttcttttaa cgtgcacaaa aaaagttgtg acacaaaact tgtcacaact tttttcaaat
     4681 aaacg
//
LOCUS       DBNICI010000181         4625 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.185, whole genome shotgun sequence.
ACCESSION   DBNICI010000181 DBNICI010000000
VERSION     DBNICI010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.185"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(586..1404)
                     /locus_tag="AC1ZCC_002547"
     CDS             complement(586..1404)
                     /locus_tag="AC1ZCC_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIQ1630762.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1565..2254
                     /locus_tag="AC1ZCC_002548"
     CDS             1565..2254
                     /locus_tag="AC1ZCC_002548"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047465 - N-acylglucosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HIQ1630763.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2268..3770
                     /locus_tag="AC1ZCC_002549"
     CDS             2268..3770
                     /locus_tag="AC1ZCC_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIQ1630764.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            3783..4259
                     /locus_tag="AC1ZCC_002550"
     CDS             3783..4259
                     /locus_tag="AC1ZCC_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIQ1630765.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
BASE COUNT         1546 a          665 c          817 g         1597 t
ORIGIN      
        1 ttaatataaa aatcaaaagg atcataaact tttaacaagt gatgaaatgg gtggaaattg
       61 attcttatag acagtttgtc tcataacatt gaagtatttt gcaattttag cattgaaaca
      121 cttttttaat cttcaacgtt gttcttataa acaagatgat tgggaatctt ttgaattaac
      181 actgtatagc ttagaacacc ctttatagaa aacattcgct tagcaatctt aataattcct
      241 tgtgcccgat gtcgtttaga ttcagtaggt tcctgctcat cgatcatgga aaaaatttga
      301 aggatcagat ccttaataaa tttatttaag agaggatgat ccattgcttc atccatactc
      361 gataaactag tgataatgag ctacacgttt ttctttcaag ttgatcaata gcttccacga
      421 taaaaatatc tttagataaa atcaagaacc gtaggaacta cagggatatt tggtcaataa
      481 gagccaccgc tattagtaaa aaataagcta tcaagtatgc tctcatccca agaattgcct
      541 acttcaatcg ttaaaaaccg ttaggtttag ctaagtgggg gtagttcact caatactttt
      601 atcaataata gagcgcaaaa ctttttctct taattcaact gaatcaatat tattgttttg
      661 ttcataacca tgaactaata gatcacaaat ataaagttgt gatatttttc cagctaaaga
      721 tccaccatta agaaattctt caatggctgt ttgtaaaact aagtctgcag actttcctat
      781 aggtgaatga atatagttag ttatagcaat aatttttgcg ccattttttt tagctatttt
      841 caatgaatca taagtatctt tcgttttccc agataaagaa aaaatgatta ctaaatcatt
      901 aactgttaat aaagaagcga cttgggcttg aaaatgaggg tctagtacag cttttgcttg
      961 aactcctacg cgaagaaaca tattttctaa atcaagacta gtatttccac tagatcccac
     1021 tccaaatata tatatgtttt ttgattgagt gatcatttta atggcttcat tcaatttttc
     1081 ttcgtctaaa agcctaatag tggaatagag agcttctgtt aaactattag caatttcatc
     1141 atagtacttt acactagtcg gatgttcttt tttttgacta aagtcttcct ttgcaatttc
     1201 aatttttaaa tcagaaaaac cagaaaaacc aattttttga caaaagcgaa taatagtagc
     1261 atcgccaact tttgtgtttt tcttaatatc actcatcgta ctataaataa tagttttccc
     1321 agaatttaaa ataaaatcag cgactttttt ttcagacttc gttaaaaatg gataattttc
     1381 tttaattaaa tagatataac tcatacaaac tcctctacaa tttcaacttt tctccattat
     1441 actccaaaat aataaaaaaa actcctaaat actccatttt tatctttact tttatgaaag
     1501 cggatgctat attgtttttg gagttgttat ccaaaaataa taaaaaattg gagtgtttag
     1561 gataatggat aaaaagaggt ttttagatct tatttcaaaa ggtttgattg tttcctgtca
     1621 agcattacct ggtgaaccat tttacaaaga ggaagaaagt ttaatgcctt attttgcttt
     1681 ggctgctcaa agggctggag cagttggtat acgtgcaaat tctgttcgag atattattga
     1741 aattagaaag tgtgtatcat taccaatgat tggaattatt aaaagagatt atccaccaga
     1801 atctccattc attactgcca ctatgagaga agtagatgaa ttagtagaaa ccggtgttga
     1861 ggtaatcgct cttgattgca ctcttagaaa tcgacatgat ggactatcta taaattcttt
     1921 tattactaaa ataaaagaaa aatatcctca tcagttattt atggcagata tttctacatt
     1981 tgaagaaggc aaaaatgcat ataaagcagg aatagatttt gtaggaacta ctttaagtgg
     2041 atatactgag gaaagtaaaa aacaagatgg gccagatata gatttgatta aaaaattggt
     2101 agaagaaggg atttgtgtaa ttgctgaggg aaggattcat actccagaac aagcaaaaga
     2161 gataaaaaaa ataggagttg caggtattgt tgtgggtggt gctattactc gaccacagga
     2221 aattgcatca agatttgtta aagcattaag ttagggggag aaataaaatg tttaaaaaat
     2281 tttcacaatt aggacgcgct ttcatgttac caattgctat attaccagta gccggattac
     2341 tattgggact gggaggagcg ttaacaaatg agagtgctat aaatgcttat ccatttttag
     2401 atcaaccgtg gttgcataca attttaagta ttatgagtta tgctggaaat gcagtttttg
     2461 caaacctagc actaattttt gcaattggta ttgctgtagg acttgcaaat ggagataaag
     2521 gaacagctgg tttagctggt ggagtagctt atctagtata tactgcaact attagtgggt
     2581 ttcttgaact tttttcagca aaagatgcaa cactagatac aggtgtagtg ggatcaattg
     2641 ttgttggtgg tactgttgca tttttacaca atcgttatag aaaaattgaa ttaccacaat
     2701 tcttaggttt ttttggagga tcaagattta ttccgattgt ttctagttta gctgcaatca
     2761 ttattggttc tttcttttat ttaatttggc caccaattca aaatggttta gtagttgctg
     2821 gtgaacaaat tgcccaaatg ggtagtttag gaactttttt atatggtttt ttattgcgtt
     2881 taacaggtgc tgttggacta caccacacca tttacccttt gttctggtat acatctttag
     2941 gtggtaccga aactgtagct ggatcaacaa ttgctggagc ccaaaatatt ttctttgccc
     3001 aactagctga tccaaaccac actggtctgt ttacttatgg tactagattt tttgcaggaa
     3061 gatttgcaac aatgatgttt ggattaccag cagcatgtta tgcaatgtat cgtgcaattc
     3121 ctaagaaaaa tcgtaaaaag aatggtgggc tttattttag cggggccctt acatcatttt
     3181 taactggaat tactgaacca gttgaatata tgtttttatt tgttgctcct tggctttatg
     3241 tgattcatgc attcttagat ggattatctt tttattttgc tgatatctta aatattcgtg
     3301 tagggaattc cttttcaggt ggtttgatcg actttttatt attcggtgta cttcaaggga
     3361 atgacaaaac taattggtta aaattaattc cttttggtat tgcatgggca tttatatatt
     3421 tctttgtttt tacattcttt atcaaaaaat ttaaggtagc tattcctgga atggaagaag
     3481 aatcagaagt tcattctgaa aaagtaataa acaaaaatac atcattacat gaagagtctc
     3541 ttatcattat tgaagctctt ggtggaaaag aaaatattga gtctgttgaa gcttgtgcaa
     3601 ctcgtttgcg tgttgctgtt acgaatgggg agattattca aaaacaaaca ataaaagaca
     3661 ctggtgcaac tgctgtcttc gaagtgaagg gtggcatcca agctgttttt ggagggaaag
     3721 ctgacttatt aagtcaagaa atcaatcaaa tattaggaaa agataattag gaggttttta
     3781 atatgtttga aattttcaaa aagaaaaaac aagaaacttt ttatgcacct tgttccggaa
     3841 aaatcatttc catcacagaa gtaaatgatc ctgtcttttc acaaaaatta ctaggtgatg
     3901 gttttgccgt catacctgat ggttcaaaaa tttatgctcc attggctggt cgagtaacaa
     3961 atatatttcc tactaaacat gctattaata taaaaagcaa ctcaggcatt gattatttga
     4021 ttcatattgg aattgataca gttgaattac aaggagagcc tttttcgatt tgtgttcaag
     4081 aaaatgatga ggttactcct gaaacctcgt tggttgatgt ggattttgac aaaataataa
     4141 aatctggtaa agatacttct gttatagttg tttttccgga gaaaaatcag ttcaatgatt
     4201 taataataga taatggtcga attgctcatg ggaattcatg tggaaaaatc actttttaaa
     4261 aaagtgtata taaaaagcta taaaataagc taaataggat atttgtcaaa tgaagttggt
     4321 gaacagaaag aactgttttc tttctgttca tttttttatt ttattagtaa tgattcccca
     4381 tgccaaatca tgatccgtaa tttaaagtta taaaagcttc gaaatccatt ggcatttcct
     4441 tggaatacct tgatatgatt attcaaatat aaaatataga gagaagagag agagggaaga
     4501 tatttataaa gggcaaagtt atgtttactc aacttaggaa atagaaatag atcaatttat
     4561 attctcttgt caatcgaata tgaaccttag cactatatac caaatatagt aaaatgctga
     4621 aagat
//
LOCUS       DBNICI010000182         4619 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.186, whole genome shotgun sequence.
ACCESSION   DBNICI010000182 DBNICI010000000
VERSION     DBNICI010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.186"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(29..1261)
                     /locus_tag="AC1ZCC_002551"
     CDS             complement(29..1261)
                     /locus_tag="AC1ZCC_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIQ1630766.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1516..2085
                     /locus_tag="AC1ZCC_002552"
     CDS             1516..2085
                     /locus_tag="AC1ZCC_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIQ1630767.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2263..2703)
                     /locus_tag="AC1ZCC_002553"
     CDS             complement(2263..2703)
                     /locus_tag="AC1ZCC_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HIQ1630768.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2861..3625
                     /gene="map"
                     /locus_tag="AC1ZCC_002554"
     CDS             2861..3625
                     /gene="map"
                     /locus_tag="AC1ZCC_002554"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIQ1630769.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3657..4580
                     /locus_tag="AC1ZCC_002555"
     CDS             3657..4580
                     /locus_tag="AC1ZCC_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIQ1630770.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
BASE COUNT         1397 a          857 c          912 g         1453 t
ORIGIN      
        1 tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta
       61 ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat
      121 ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg
      181 taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt
      241 catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta
      301 caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga
      361 catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca
      421 tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta
      481 ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt
      541 ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa
      601 tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt
      661 ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat
      721 agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca
      781 gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga
      841 ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat
      901 aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc
      961 tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta
     1021 cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag
     1081 tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag
     1141 caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta
     1201 cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca
     1261 taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta
     1321 tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc
     1381 atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac taattttgca
     1441 tatcaaaaaa aacatgttac aatgaaatta atcaatggtg gaagcgttat aaaaaaatta
     1501 aggagattaa aaaatatggc aagaaaaaag acgattacta gagaacagat tttggcagct
     1561 gcttatgaag tagctgccac cgatggattt acacgattta ctgcgagaaa catcgcgaac
     1621 aaaatgaatt gttccacgca accgatttat cttgagttta aaaatatgga tgacttgaga
     1681 caagctcttt ttgagcagat ctatgatcat ttacaatatg aagtgttcac tgttgagcat
     1741 acagggaata cgattatcga tcttgctttt aattatatcc attttgcaag aagagagaag
     1801 aagttgtatc gttcactgta cttagaagaa tatggtggag gaaaagagat gcagaatttc
     1861 tcttatactt actttgtgaa tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa
     1921 gagcagatcg aatctttgta taatgggaca tggatcatag ctactgggat cgcagctttg
     1981 atgtcttcaa gcatcatcca tccaactgat gaacagattg aaaagatgat ccaagacagt
     2041 atagatgcta tcttgaaatt caaagaacca atcgaaatcg attgaatata ctcaaaagtt
     2101 ccacaaatca gtaggtaaca agagatgtgt caaaataagg aaaagttcgt ttttcttaca
     2161 gcgtatgcat ttttaactcc cgaagaactc ttccatgaat gcaatgaata aaagagccat
     2221 acagattgaa taaatctgga tggctctttt attgcaagta tattaaattt ttgcgactag
     2281 ttctttagcg aaagcttcta aattttcgat atcttcttct tcagcattca gatccacttt
     2341 aacattttcg gctccttttg atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac
     2401 cgatttgcag aattcatcgt aaaaagtatc accagatccg cagacaccgt aaatttttcc
     2461 caatagatcc aattcttgca agtcttcata aaagtctacg atctcatcag gcagatcgcc
     2521 atcatcgtat gtataagtag ctactacgca aatatctgcc tcctcgaatt catctgcatc
     2581 tacttgggta cattcgttga tttcgacttc gatatccaga ttttcaaatg cttctgcaac
     2641 aatatcggcg atttcttcag tgtttcctgt catgctagca tatacgattt ttgctaaagc
     2701 catgatgtgc ctcctcgttt ataattattt aacaattatg aattatacca aaacatcatt
     2761 gaaaagaaaa taaaacctag agacacctgg ggaatgtcat ttggctaaat gtatgccgct
     2821 atgttaaaat gagagggatt tgtaaaaagg agacgaaaat atgattactt taaaatcagc
     2881 acgtgaaatt gaaatgatgg cagaatctgg agctttatta gcagatgtac ataaaaatct
     2941 gcgtgatttt ataaaaccag gaatcacaag ttgggatatt gaggtatttg tcagaaacta
     3001 tatcgagagt catggcggaa ttgctgcgca aattggcttt gaagggtatg aatatgcgac
     3061 ttgtatcagt ataaatgatg aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa
     3121 cggtgatttg gtaaaagtcg atatgtgtat cgatttgaaa ggcggcattt ctgattcttg
     3181 ttgggcctat gtagttggag aatctacacc tgagatcgac cgattgatgg aagtaacgaa
     3241 gaaagctttg tacatcggta ttgagcaagc acaagtaggg aatcggattg gtgacatcgg
     3301 tcatgctatc caaacttatg tagaaggcga aaatctagcg atcgttcgtg attttattgg
     3361 tcatggcgtt ggtcctacga ttcatgaaga accagcagtt cctcattacg gagaagctgg
     3421 aaaagggttg cggttgaaag aaggcatggt tatcacgatc gaacctatgg ttaataccgg
     3481 aacttggaaa atgaaaatgg atcctaacgg ctggactgcg tatacaagag acggcggact
     3541 ttcttgccaa tatgaacata cattggctat tactaaagat ggtccaagaa ttttgacatc
     3601 acaaggagaa gaaggcactt attgatctat ccagtcaata ggtacgggag aggtacatga
     3661 aagtttggaa caaaataaaa caaaatgaaa atctgatgcg ctttattgag acgactcaaa
     3721 agcgcatcac tgattcagaa atgagtacga cctctgtcgt cgttgcttat tatttactct
     3781 tgtctttatt tccattgatt attgcttttg gtaatatttt accgtttctc cacatcgatc
     3841 aagagactgt actgacctat ttacgagaag tgattcctga gaccgtttac cagtttatcg
     3901 gtcctgcaat caaagacctt ttgacacaaa gttcaggagg cttgttatcc gtttctgcat
     3961 tagcggcttt atggtctgca agtcaaagta tcaatgcttt gcaaattgcg atgaataaag
     4021 cgtatggcgt agaaaataga aaaaattttt tgatcgtccg tttcttctca ctaattgtca
     4081 ttgtcttgtt catgattgct attagtgggg taactatcgt tcttgggttg ggacagctga
     4141 ttttggatgc tattcagcca atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa
     4201 ctttgaagtg gccaatcaca ttggtagctt tatttgtcat catgttcttg atttatttaa
     4261 ttgtccctaa tgctcagttg aagttgaaga cgatcattcc gggcacgatc ttcgctactg
     4321 tcggctggat gctgttatca caagtgttta gtatctatgc taggtatttt gcaacgagga
     4381 tcagcggtta tcagatcata ggaagtttta ttgtactcat gctttggctt aattttgctg
     4441 ctacgatcat cattttgggc ggaatcatca atgcagttgt tcaagaatac gtgacgggtc
     4501 atcaaatcaa aaaacgtcga gatccaagcc gaaaatggtt caataaattg aaaactaaat
     4561 tttctcataa aaaaaagtag aaacaagagt tcttattgta aatttatatc agtaattta
//
LOCUS       DBNICI010000183         4609 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.187, whole genome shotgun sequence.
ACCESSION   DBNICI010000183 DBNICI010000000
VERSION     DBNICI010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.187"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(61..189)
                     /locus_tag="AC1ZCC_002556"
     CDS             complement(61..189)
                     /locus_tag="AC1ZCC_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630771.1"
                     /translation="MNRAEALKIGVIIANRWWRHNKPNILSQQHIDKQKAWQQIKK"
     gene            complement(182..889)
                     /locus_tag="AC1ZCC_002557"
     CDS             complement(182..889)
                     /locus_tag="AC1ZCC_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HIQ1630772.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFDKKHRHVLTAIDELKEGVAEN
                     WADLFWEDTYVHPQNKQSYRIIYMNRDGFSLLAMGFTGKKALSFKLQYIEAFNKMEKE
                     LKDQLPSIPATKREMALLALAANEETNERVDAIESDLNDLKNNQLLAEPDYRTISNMV
                     RNKIRVICNQQHLNSKAKAELFKDLNGGIKRITGAVARNRIKAKQFDDVIDFINNWMP
                     STATMTIIKQMELLEDE"
     gene            934..1413
                     /locus_tag="AC1ZCC_002558"
     CDS             934..1413
                     /locus_tag="AC1ZCC_002558"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630773.1"
                     /translation="MSRKIDNIILLTTIFYLVIGATLSLLSFLSNSSLIVLNQKSILT
                     EFAHIDYFSFFKTGLICLFLSSFILYIGTTFCLMVLNLKMFIIEKWIVIIAKIFSSIS
                     IIIPMTLTFSKEQFNVATTFVSFIALFSFAVPKTFQSKSSNAKVNVENTDRKNDESY"
     gene            complement(1472..1642)
                     /locus_tag="AC1ZCC_002559"
     CDS             complement(1472..1642)
                     /locus_tag="AC1ZCC_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630774.1"
                     /translation="MGKFNRTLVFSAPLIIYALGLWGSRQALIGTIVYMLWIFIGLDE
                     AEYRAKKPVGRD"
     gene            complement(1654..1920)
                     /locus_tag="AC1ZCC_002560"
     CDS             complement(1654..1920)
                     /locus_tag="AC1ZCC_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630775.1"
                     /translation="MNILSEEFLTRLRIAIVEVVKDALSQLSKKNLSETRYLKKIEAR
                     KYVGGVNDQGFEKLIAHGLKEIRIDGFLRYDKKDIDELMAKYKI"
     gene            complement(1931..2122)
                     /locus_tag="AC1ZCC_002561"
     CDS             complement(1931..2122)
                     /locus_tag="AC1ZCC_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630776.1"
                     /translation="MSNLDNGRAAIKEFMKENGITMDDLATAYGHTRVRMQQILDGHW
                     SGPKANRTILEIIRDYRIR"
     gene            2413..2835
                     /locus_tag="AC1ZCC_002562"
     CDS             2413..2835
                     /locus_tag="AC1ZCC_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1630777.1"
                     /translation="MTTFERIKELAKNKNKNLQQVAKDLKYGENYFYSLNAGKQPTAE
                     KLQEIADYFDVSVDYLLGRTEKKRYYDLTEKDERDIQKELQSLIDDLSNADGMAFSKK
                     DNEMSEATREALIISLENALRIAKIEAKKKYTPKKYRD"
     gene            2852..3274
                     /locus_tag="AC1ZCC_002563"
     CDS             2852..3274
                     /locus_tag="AC1ZCC_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HIQ1630778.1"
                     /translation="MYLPQIDNKINKLVKLYQTRNPFRIARELKILVLEEDLGEIYGY
                     YSKFKQIKMIHINSSLDEMGKWITCSHELGHCVLHPNENTPMLSKKTLVSELKIEKEA
                     NYFATHLTIDPTLDGFDHMTKYEKLVCFGLPDEFDRFL"
     gene            3303..3503
                     /locus_tag="AC1ZCC_002564"
     CDS             3303..3503
                     /locus_tag="AC1ZCC_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630779.1"
                     /translation="MKIGMRKPSIKKSISARTTGKAKRKVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
     gene            3590..4039
                     /locus_tag="AC1ZCC_002565"
     CDS             3590..4039
                     /locus_tag="AC1ZCC_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HIQ1630780.1"
                     /translation="MKKVITSLLVFASLLLFTACGKKVTTEDLKANDWLIEATKDDEP
                     NMIVSFSDHVVSFSVDTSSMKSTSSNDWEAMGEEFAKSLVDQMNYKFEYTLEGDVMTW
                     KDDKDKDNDAKYTVSKEDKNIILTPEKPNKSDDNEKLVLKPYKKEKN"
     gene            4341..4478
                     /locus_tag="AC1ZCC_002566"
     CDS             4341..4478
                     /locus_tag="AC1ZCC_002566"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIQ1630781.1"
                     /translation="MTLQPGEGTKQIAERAGITVDQLFALNGMDPNNYMLYPGQELRV
                     K"
BASE COUNT         1560 a          818 c          746 g         1485 t
ORIGIN      
        1 catgtggtaa catctcctta gatgtatttt ctttgtgact cattgctttg gtcggctgag
       61 tcactttttt atttgttgcc atgctttttg cttatcaata tgttgttggc ttaggatgtt
      121 tggtttattg tgtctccacc agcgattagc aattattacg cctattttta gcgcttcagc
      181 tctattcatc ctctaacaac tccatttgct taataattgt catcgtcgct gtagaaggca
      241 tccaattgtt aataaaatcg ataacatcat cgaactgttt tgccttgata cgatttctag
      301 ctactgctcc agtgattcgc ttaattccgc cattcaagtc tttaaacaat tctgcttttg
      361 ctttgctatt taaatgctgc tgattacaaa taactctaat tttgttgcga accatattag
      421 aaattgttcg gtaatctggt tcagctagaa gttgattgtt cttcaaatcg tttaaatcag
      481 actcgatagc gtctactctt tcattcgttt cttcgtttgc agctagagca agtaatgcca
      541 tttctcgttt agttgctgga attgatggca attggtcttt taattctttt tccatcttgt
      601 tgaaggcctc aatatattga agtttaaaac ttaaagcttt cttaccagtg aagcccattg
      661 ctaatagaga gaagccgtct ctattcatat aaataattcg gtatgattgt ttattttgag
      721 gatgaacata agtatcttcc caaaataggt ctgcccaatt ttcggcaacc ccctctttta
      781 gttcatcaat agccgtcaaa acatgtctgt gctttttatc gaagctttct gcaacttgta
      841 agctcgtagt cacagcttct ttatttttca aaattactaa ttcttgcatt atttcttctc
      901 tcctttttga tataattctg gtggggtgaa aacatgagca gaaaaattga taatataata
      961 ttgttaacaa ctatttttta tttagtaatc ggagcaactc tttccttact gtcatttttg
     1021 tccaacagta gtttgattgt gttaaatcaa aaaagcatat taacagaatt tgcccacata
     1081 gattacttct ctttttttaa aactggacta atatgtttat ttctatcttc atttatcctc
     1141 tatataggaa ctacattttg tctaatggtg cttaatttaa aaatgtttat aattgaaaaa
     1201 tggatagtta ttatcgcaaa gattttctca tcgatatcaa ttatcattcc aatgacattg
     1261 acgttttcta aagagcaatt taacgtagct actacattcg tatcttttat agctctattt
     1321 tcgtttgctg ttcctaaaac ttttcaaagc aaatctagca acgcaaaggt taatgtagaa
     1381 aacactgata gaaaaaatga tgaaagttat taaaaaaaat aattctttca tttattgtca
     1441 aacctttctt atagacatta gactttttta gtcagtccct cccgactggc tttttcgctc
     1501 tgtactcagc ttcatcaagc ccaatgaaaa tccaaagcat gtaaacgatc gtgccgatta
     1561 atgcttgtct actaccccat agacctaaag cgtagataat tagtggcgcg ctgaatacta
     1621 atgttcgatt aaactttccc atgttgacct cctttaaatt ttgtatttag ccatcagttc
     1681 atcgatgtcc tttttgtcat atcttaaaaa gccatctata cgaatttctt ttaaaccgtg
     1741 agctattaac ttctcaaaac cttgatcgtt tacacctccc acatattttc tagcttcgat
     1801 cttttttaaa tatcgtgttt ctgacaaatt cttttttgaa agttgactaa gtgcgtcctt
     1861 tacaacttca acaattgcaa ttcttaaacg agtcaaaaac tcttcgctta gaatattcat
     1921 gacttgcctc ctatcgaatt ctgtaatcac ggatgatctc tagaatagtt ctgtttgcct
     1981 ttggtccact ccagtgtcca tcaagaattt gttgcattcg tacacgcgtg tgaccataag
     2041 cagtagccaa atcatccata gttattccat tctctttcat aaactctttg atcgccgcac
     2101 gaccgttatc caagtttgac attaacccac ttcctttttt tataaatttg taagttaaaa
     2161 tgatagaaaa aatataattt ccattgacat ttttacacta tagagtaaaa tgaatacata
     2221 gagaaataag cctataacaa aaccttttat ttgcacttgg tcgccaaact ttatgctata
     2281 aggtgtgttt ttagtttgct tttttctatc aaattaactt acggataaat aataactcta
     2341 tatcgtaaaa ttgtaaagct atttttactc tttttcgtaa attatttttt cgtgttggaa
     2401 aggaacgttg gaatgacaac atttgaaaga ataaaagaac tagcgaaaaa caagaataaa
     2461 aaccttcaac aagtcgctaa agatttgaag tacggagaga attactttta tagtttaaac
     2521 gcaggaaaac agccaactgc cgaaaagtta caagaaattg ctgactactt cgacgtatct
     2581 gtagattatt tgctaggacg tactgagaaa aaaagatact atgatttaac agaaaaagac
     2641 gagcgtgata tccaaaaaga attgcagtca ttaatcgacg atttatctaa tgctgatgga
     2701 atggcttttt cgaagaaaga taatgaaatg agtgaagcga cacgtgaagc tctcattatt
     2761 tctttagaga atgctttgcg aatagctaaa atagaagcta agaaaaaata cacacctaaa
     2821 aaatatcgcg attaaaagga gtacgttgtt tatgtattta cctcaaattg ataacaaaat
     2881 taataagctg gtaaaactat atcaaacacg caatcctttt agaattgcac gagaattgaa
     2941 gatactagtt ttagaagaag atttgggaga aatttatggt tactatagta aattcaaaca
     3001 gataaaaatg atccacataa attctagttt agatgagatg ggaaaatgga taacttgtag
     3061 tcatgaatta ggccactgtg ttttgcatcc gaatgaaaac acacctatgt tgtctaaaaa
     3121 aacgctagta tctgaattaa aaattgaaaa agaagcaaat tatttcgcaa ctcatctgac
     3181 tattgatcca actttagacg gctttgatca tatgacaaaa tatgaaaagc tcgtctgctt
     3241 tggattacca gatgaattcg atcgtttctt gtgaattata atacaacgta aggaggatat
     3301 agatgaaaat aggaatgaga aaaccaagta taaaaaaatc cataagcgct agaaccactg
     3361 gaaaagcaaa aagaaaagta aaaaaagcag ttattcctgg ttatgggaaa aaaggtagcg
     3421 gctggattaa agacccaaaa aaggctgctt acaataaagt atacaagaaa actacattca
     3481 gtttttggga tttatttaaa taaaaaaagc ccgtgcagca acacggactc gaaaccttat
     3541 ttctaagatt ccccaataaa aatcatatca tagaaatgag gaataaaaaa tgaaaaaagt
     3601 aataacaagt ttattagttt ttgcttctct gttacttttc actgcttgtg gtaaaaaagt
     3661 aactacagaa gatttaaaag ctaatgattg gttgattgaa gcaaccaaag atgacgaacc
     3721 taatatgatc gtatcatttt ctgatcacgt cgtatctttt agtgttgaca caagttctat
     3781 gaaatcaact tcaagtaacg attgggaggc tatgggagaa gaatttgcta aaagcttagt
     3841 cgaccaaatg aattacaaat ttgagtatac acttgaaggc gatgttatga cttggaaaga
     3901 tgataaagac aaagataatg atgccaaata tactgtcagc aaagaagata aaaatattat
     3961 tttaactcca gaaaagccta acaaatctga tgataatgag aaattagttt tgaaacccta
     4021 caaaaaggaa aaaaactaaa gaatctagaa caactactag ctcaacaagt agcaacgaac
     4081 aatcatcttc gactacaaca gaaagtacta ttgcatcttc tagtactgta gaacaagctg
     4141 ctattgcgca gaatacgcct cctgaaacta gtagtactta ctctgctgaa agtgtagaaa
     4201 actatgcaga agctactcct agttcaagtg ttgaacaagt ttcacccact cccagttcaa
     4261 cggagcaatc agtaccacaa gattatgtac cagctaatca agctcaacca acggcacctt
     4321 cttcatctga aaccgaaacc ttgactttgc aacctggtga agggaccaaa caaatagctg
     4381 aacgagctgg aattacggta gatcaactgt ttgcattaaa cggtatggat ccaaacaact
     4441 acatgcttta tccaggacaa gaactaagag tgaaataaaa aaagcacatt tgccgcccga
     4501 ccaagaacgc aaatgtgcaa agagctacgg taaagtaggt ctatttgttg taccctattt
     4561 taccatctcg aaatccgctg tacaaccgaa caaacgtacg aaaggaaag
//
LOCUS       DBNICI010000184         4594 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.188, whole genome shotgun sequence.
ACCESSION   DBNICI010000184 DBNICI010000000
VERSION     DBNICI010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.188"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            190..2859
                     /locus_tag="AC1ZCC_002567"
     CDS             190..2859
                     /locus_tag="AC1ZCC_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HIQ1630782.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2950..3570)
                     /locus_tag="AC1ZCC_002568"
     CDS             complement(2950..3570)
                     /locus_tag="AC1ZCC_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIQ1630783.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3684..4223
                     /locus_tag="AC1ZCC_002569"
     CDS             3684..4223
                     /locus_tag="AC1ZCC_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630784.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4220..4558
                     /locus_tag="AC1ZCC_002570"
     CDS             4220..4558
                     /locus_tag="AC1ZCC_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HIQ1630785.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1424 a          790 c          860 g         1520 t
ORIGIN      
        1 aaaaattttt aagggtggga cttttgattc ctaccctttt tttgcgttta tttttgttat
       61 acgaacaaat tatgaagtta tttattctaa attttatttt tttgtgaaaa ttagactctt
      121 tttaagcaga gaaaaacaag gatatgataa actgaataca acttcataga aatttggtgg
      181 aaaataaata tgaaaaaaaa tttaaagtca ttctttagag aaaacggacg ctacgcagca
      241 gctagtttcc tgattccgtt tttgatttta gcacttgttt atctaagtat cgggatctat
      301 cctggaagca gcagaagtat tttggcaagt gatgcctttt cacagttttc taattttcat
      361 gccagcttcc gaaatgttct tcttggaaag caaagtattt tttatacatg gaatgcttct
      421 ctaggattga attatttatc tttaatttct tactatctag gcgggatctt cacgccactg
      481 gtgttctttt ttccaaacca gatgatgcca gatgcactgt atgttctgac tttgcttaag
      541 gtcggctgtg ccggattggc tttttggttt cttgcacgta cgttcaagat tcctcgctgg
      601 gggcatgttg cacttagcgt cagttacgca ttgatctcat ttatcactgc acattcggag
      661 atcatcatgt ggctggatgc atttgtttat cttccattag tgattttggg gattcatcgt
      721 gttatggatc ttaaacggcc gaaactttta tttatcagtt atttactctt gtttttatcc
      781 agtttttata tgggttttat gattggcgta ttttctttct tatattttat cgcccgtcta
      841 ttgacaaact ggtcagaata taaaaaaacg attgttcctt atggtatcac ttctttactg
      901 gcaggcggcg cttcaatgat cattgtattg ccagcagtgc tggatcttcg gtcaaatggc
      961 gaggcactga gtcagatcac tacgttgaaa acagaagcga cttcttatct ggatctaatc
     1021 atgaaaaata tgattggtgt ttatgacacg acaaaatacg gatcgattcc gttcgtttac
     1081 gcaggtctgc taccattagt cctctgtctg ttttattttg ccagtcggaa agtaccgttg
     1141 aaaaataaat ttttgtacgg cagtcttttc gccatactta tcgcaagttt ctatcttgtt
     1201 ccattgaatt tgttctggca tggtatgcat gcaccgaata tgtttttatt ccgttatgcc
     1261 tatctgttct ctttcttagt gctcctgctt gcaggaaaag gatgggaaaa gatagaacaa
     1321 gacgatcgtg ggctgttgtt aggaatcatt ttggtcgtag ctgctctctt tgcggtggca
     1381 tggggcatca aacctgaagg aagctacacc tatgtgacga tgacttcctt tgtattgaca
     1441 gtggttttct tagggattta tgcttttgct gtgggatttt atcagcataa aaaactttca
     1501 ttcaatcatc tgagtatatt gttattattg atcatgtctg ctgaagcact ggtcaatacg
     1561 aacagtatga tccatggcat tcttgatgat tggaattatg cttcccgcag tttatatact
     1621 gatccttatc ctgacttgaa agctcttgta aatgaaacaa aaaaagattc cgactcgttt
     1681 taccgcttgg aaaatctgga tccagtatct gcaaacgatt cgatcaacta tggttacagt
     1741 ggtatcagtt tgttttcttc tataagaaat cgtaattctt cctcttacct ggataaatta
     1801 ggtttccgtt caagagggac aaatttgaat atccgttata acaataatac tttgctaatg
     1861 gatgggttta caggaatcaa atataacatt tctaaaaacg aaagcacgtt cagtaaatat
     1921 ggcttccgcc aagctggtca aagtggcgac tatacactgt atcagaatgt caatgcattg
     1981 ccactagcat ttactgcacc gttgtccgtt aatgatgttg agcagccagt gacagataat
     2041 ttgaccagtc agacaaactt gatcaataca ttgtctggtt taaatgaaca gtattttaca
     2101 ttctatacac ctactttgaa gaatcagttg aatacgacga tcacaaagac aacgactggc
     2161 gtcacttact cagaaacagc agataaccag ccaaaagagc tcacttggga agtccatgtg
     2221 cctgcaaata cacaagctta cttaagtttg ttcccaacca attttgcaca attagaaagt
     2281 tctagtgcga cgatcactgt caatggtaca tcgcgaaaaa cgcaaatcaa catttctggt
     2341 caatactatg atcttggcta ttatcctcaa gagacaacag tgacatttac tgcaagtttt
     2401 tatggaacga aagaagtaag tttcatggag cctaaagtcg ttggtctaga tgttgtagcc
     2461 tatcaagccg caatgaatca gatcaaagaa aaaggtgtcg aaatggaagc cactggcaga
     2521 aaagcttctg ggactgtaaa taccgctgaa gattccatgt tggtgacaac tattccttat
     2581 gacaaaggat ggaaagctaa aattgatggg aaaccagtca aggtcagcaa tttcaaagac
     2641 gcattcttga tgataaaagt tccagcaggt aaacatactg tcactttcag ttacttgcct
     2701 gaaggattta cgataggtgc aatccttttt gtcctatgta ttgcattgtt tattctttat
     2761 gtctggtacc ttgaccgcaa tcgccccttt tatggcacga ttgatccagc aatagaagaa
     2821 aaaccgcgaa atacacacag aagaagacga agaaaataaa gaaacgatgg aatctgtgtc
     2881 actcttctat ttaaatggca aaaatttcaa tctttaaatg acaaaaacca cctgattttt
     2941 ggtggttttt tatataccta tgactaaact atctcttgct gcttcgacaa cttctacagt
     3001 gatcttatcc atttgattga ttcttaaaat ccgatcaatc tgcgcaaata aacgatgaat
     3061 aagcctaaaa tttccttttg tgatcttaat gatagttgtg atagcctcat aatcagaaaa
     3121 atcttctagc ttgatatcaa ttcctaaatc tgcccatttg tattccaaaa tatgatgtgt
     3181 ttcgtcctta ctaaggttat caaattcatg tgcaaatcct atgcgagagt aaagctgcgg
     3241 ataacgagac aatctttttt caataccagg cataccgata agaaccattg ccagatcatt
     3301 tctatcgtaa atatctctta gctgttctaa atgctgaagc tttaaacgat caatttcatc
     3361 gacaataatt aagtcaattg cttcataggt tttaggcaca ggaggatctt cttgattgta
     3421 ttttagtacc atatgcattg ctttagtcag acctattcta ctagttattg tgttaatatc
     3481 cgaactgaca cgagacatct tttcagctgg tacagtataa aaaactttat ttacacttaa
     3541 tatttcatct gttgcattta tcaagaacaa gaaaaagtct gaatattcaa aaaaaataac
     3601 ggtgtacatt tttaaaagga gatgtataat cgagatttag aaagcgtttt cctcaaaatt
     3661 taatgaatta ggaagtgatt actatgaaaa aattatttag cgcacttttt attctgttat
     3721 ttggctttat tggtattggg acttctggaa atatagttaa agctagcacg atggttgaac
     3781 aaacaagtga aatggcaaaa acttttagtc aattaacacc agaagaacaa agttacttca
     3841 aatccaaagg atttagtgat aaagatactt tttacactca aaagatagag cctcaagatc
     3901 cgaaaacacg tttttctgca aatgtattgc ggttaaccgc atctactaaa aaaattacta
     3961 atacgcaggg gcggacagaa tatattataa caagtcccaa ttcggcgatg tatcgtattt
     4021 ctacacgctt gagtttaggt agtgcaaaat catatactca aacagtaaat ctagctaatt
     4081 cttatggtta tggtggagga atgtatttta catttactgg gaaaagacaa tattacagtg
     4141 taaaattagc tatgcaagtt tataatacat ttggtgtagg gtcagtctct tgcactgcgg
     4201 gaggattgac aataggaaaa tgataatttc ttactatgtc ttaaatagaa gtgatgatga
     4261 aggactcttc gtttttcaat cattacatga ggatacgatg ctaaaagagt tttataatcg
     4321 gattaagaat aaacagatag aaacaaattt atatttttta agtgactcta tcttgccttt
     4381 tattagcaac cctaaaattc ttaacttgtt gcagacaaaa gatttgaaaa gtttaccgat
     4441 tattgtcatt gatgaggaaa ttgtaaaata tggcgcttat ctaagcgtag aagaattaga
     4501 aaaattaact aacattggaa tgagtattca ggaggataat ggggtacatg gaacatagaa
     4561 tgaatatgca cagttgagaa cggaatttga catt
//
LOCUS       DBNICI010000185         4559 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.189, whole genome shotgun sequence.
ACCESSION   DBNICI010000185 DBNICI010000000
VERSION     DBNICI010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.189"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            333..1961
                     /gene="treC"
                     /locus_tag="AC1ZCC_002571"
     CDS             333..1961
                     /gene="treC"
                     /locus_tag="AC1ZCC_002571"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HIQ1630786.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(2021..3295)
                     /locus_tag="AC1ZCC_002572"
     CDS             complement(2021..3295)
                     /locus_tag="AC1ZCC_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIQ1630787.1"
                     /translation="MIDDIFAINLYHFPFIPAFVYNEFIDKGGKRVKKETDTTNRLIR
                     FLGGKTSYYVLGIVILLALIIYFFHQISFIFKPVGIIIATILPPLLFALILYYLLEPV
                     IKRLSKHLSRNLSVIIVYVIILFLLGLGSIWLIPFLEEQAKTLISSLPDLFQDFQESL
                     RQFLEKTPFADSFNQFFASIDDVTSDIAGFIGNYWESGAQRISNIFSTVTAIFITLFT
                     GPIVAFFLLRDPQKFYQSVLAIVPPAFRTDFKNLTKIANQQLGSFLKGQIIASFILGA
                     VYWVCFLLIGLEFASVLAISAGLLCIIPYIGPFIAFFPGLIIAFQDSTFMAVKFVIVW
                     FAVQLLHGDLVIPRVMGDRLQIHPITILIVLLVMGDLMGIVGVIFGIPIYTLVKLLVT
                     FIFRKFKQRYNKFYGDKGEYENSSFSEEDYFK"
     gene            3454..>4559
                     /locus_tag="AC1ZCC_002573"
     CDS             3454..>4559
                     /locus_tag="AC1ZCC_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter solute-binding protein,
                     Zn/Mn family"
                     /protein_id="HIQ1630788.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVF"
BASE COUNT         1662 a          800 c          899 g         1198 t
ORIGIN      
        1 tgaatcagag gttgtaaaaa agccgtcctg catcgagcaa aaagaatact caaataaaat
       61 tagctcctca tgttttacat ttgagaagac tgattgccgt agataaaagc ttttgaacac
      121 catttataga agaaacaaga atattttgct attttaaaca agaggataga caaatacatg
      181 tctgatctct tgttttttat tgggcaacag acctcttaga atgtataaag ataaatttta
      241 caaacgcttt cggttcagtt ataataataa atgataactt gtatatataa gttgtggtag
      301 gtaataaatc agttgaatgg aggtaaatat ccatgacatt taaagacaaa gtcatttacc
      361 aaatctatcc caaatcttac aaagacagta atggtgatgg aattggagat ttaaaaggaa
      421 tcaaagaaaa actcccgtat ctgaatgatc taggcatcga catgatttgg ctaaacccta
      481 tttatccaag ccctcaaaaa gacaacggtt atgatatctc tgattacaca gccatcgatc
      541 ctatctttgg tacgatggag gaattcgaac agctaatagc cgaagcaaag aaataccgga
      601 tagaaatcat gctcgatatg gtgctgaatc acgtttcgac agaacatgaa tggttcaaaa
      661 aagcactagc aggggaccag cattatcagg acttcttcat tttaagagaa gagccaacgg
      721 attgggtatc aaaatttggt ggaagtgctt ggtcgccatt tggagatacg gggaaatatt
      781 atttgcactt atatgatcgg acacaagctg acttgaactg gcggaaccaa gcagtaagag
      841 aggaactatt caaagttgtt cgtttttgga tggaaaaagg ggtaaaagga ttccgtttcg
      901 atgttatcaa tgttatcggt aaagacgaac aattgaaaaa taataaagaa aatgatggca
      961 aacctgaata tacggacaaa ccaatcaccc atacgtatct aaaagaattg aaccaagcaa
     1021 cgtttgggac ggattccact atcatcacag tcggtgaaat gagctctacg acgatcgaaa
     1081 attgtatctt gtatacagaa cctagtagag aagaattatc aatggtcttt aatttccatc
     1141 atttgaaagt cgattatgaa aatgggaata aatggtctac tcctccattt gattttgcag
     1201 aattaaagcg actatttcat acatggggag aaaagatgag tgagggtaac ggctggaatg
     1261 ctctattttt taataatcat gatcagccac gtgcgctcaa tcgatttgta gctatcgatc
     1321 agtatcgaaa acaaggagca gagatgctag caaccatgat ccatttgaac cgtgggacgc
     1381 cctatattta tatgggagaa gaaatcggca tgatcgaccc agactattca tcgatttccg
     1441 actatattga cgtagaaagt ttgaatgctt ataagttact ccttgaacaa ggattcacag
     1501 aaaaagaagc gtttcgtcgg atcaaagcaa aatctcgcga taactctcga acacccatgc
     1561 aatggtcaga cacagaacaa gcaggattta ctacagggaa accttggtta aaagttggag
     1621 gcaagctaga agagattaat gtagaaaaag aactatcttc tgaagattct attttgtcct
     1681 attacaagaa attgattcgg ctgagaaaaa catatccaat cgttgcacaa ggagattatc
     1741 atgcttatga agcaaatcat ccggaagttt atggctttct tcgccaattt gaaggacagc
     1801 agctgcttgt attaacaaat ttctatgata aagatacaag gatcgccatc ccagaaggat
     1861 tcacagaagc caatattctt atcgataatt attccgatac tgttcttcat caaagtctgg
     1921 agctaaaacc ataccaggca attgctttgc tgaaaaatta aaacaaaaac acaaaacgtc
     1981 gttcctacga gctattctcg taagatcgac gttttgtgtt ttatttaaaa taatcttctt
     2041 cagaaaatga agagttttca tactctcctt tatcgccata aaatttattg tagcgttgtt
     2101 tgaatttacg gaaaataaaa gtaacgagta atttgactaa cgtatagata ggaataccaa
     2161 aaataacgcc cacaattccc attagatcac ccatgacaag taaaacgatt aagatcgtaa
     2221 tcggatggat ctgtaaacga tcacccatta ctcgaggaat caccaagtca ccgtggagaa
     2281 gttgtacggc aaaccagaca atgacaaatt tgacagccat aaaagtagag tcttggaatg
     2341 caatgatcaa acctggaaag aaggcgataa acggcccaat atatggaatg atacataaca
     2401 acccagcaga aatagctaaa acactagcaa attctaatcc gatcagtaag aaacaaaccc
     2461 agtagactgc tccaaggata aatgaagcaa tgatttgccc ctttagaaat gaacccaatt
     2521 gttgattagc aatctttgtc aaatttttaa aatctgtacg aaatgctggt gggacaatcg
     2581 ctaaaactga ctgataaaat ttttgaggat ctctcaaaag gaaaaaagcg acaattggac
     2641 cagtaaataa agtaataaaa atagctgtaa cagttgaaaa aatgttgctg atccgttgtg
     2701 cgccactttc ccaataattt ccaataaatc cagctatatc agacgtcacg tcgtcaatag
     2761 aagcgaaaaa ttgattaaat gaatcggcaa aaggtgtttt ctccaaaaat tgtcggagac
     2821 tctcttgaaa atcttgaaac aaatctggca aactactgat caatgtcttt gcctgttctt
     2881 caagaaaagg aataagccat atgcttccaa gccccaataa aaataaaata atcacataaa
     2941 cgataatgac tgacaagttc ctcgaaaggt gcttgctcaa acgtttgatg actggttcca
     3001 aaagataata caagatcagt gcaaacaata atgggggcaa gatagtagcg ataatgattc
     3061 cgacaggctt gaatataaac gagatctgat ggaaaaaata aatgatcagc gcaagtaaga
     3121 taactattcc taaaacatag taagaggttt ttcctcctaa aaagcggatc aaacgattcg
     3181 ttgtatctgt ttcttttttc actctttttc ctcctttatc tataaattca ttatagacaa
     3241 aagcgggtat aaaaggaaaa tgatagaggt tgattgcaaa aatatcgtca atcattcata
     3301 taaactttct catcaattaa aataagaaaa cgcttgacag ttcttttaac catatgataa
     3361 attaaatcct gttgctaaaa cgtaactatt acggtttgcg aaaagcgaat cggaacattc
     3421 aaatgaaaga agatgagaag aggagtgtca taaatgaaac gattgatagg tatattggcc
     3481 atgctggttt tagcaggtat gttaactgct tgcggggcaa gtgggaaagc cgaggacagt
     3541 aaagaaaaat tgagcgtgat gacgaccttt tatcccatgt atgattttac aaaagcaatc
     3601 gtcggggatg aaggagaagt agagttattg attccagcag ggactgattc ccacgattat
     3661 gaaccttctg ctaaagacat ggcaaagatc caagatacag atatctttgt ttataatgat
     3721 gaaaacatgg aaacatgggt ccccgcaatc caaaaaacat tacaagaagg caatgtccat
     3781 acgataaaag cgacagaagg tatgctttta cttccgggaa gtgaagaagg acatgatcat
     3841 gaccatgaac acggggaaga aggccataca catgagctag acccacatgt atggctagct
     3901 ccaagtttag caatcaagca ggtagcaaac atacgtgatc agctgattga agcttaccca
     3961 gaaaaacagg aagtatggac aaaaaatgca gcagcctata ccgagaaact tcaagcactc
     4021 catcagcttt atcaagaaac ctttaagcaa gcaaaacaaa gatcttttgt cacgcaacat
     4081 gctgcattca actatctagc gttagaatat ggattgaatc aagtatcgat tgctggtctt
     4141 tcttctagtg aagaaccgtc tgcggcgaga attgctgaac tgaaacactt tgttaaagaa
     4201 catggaatca actatattta ttttgaagaa aacgccaaag atagtattgc cagaacgtta
     4261 gcaaacgaag caggtgtatc tttagaagta ttgaatcctt tggaaggatt gacgaatgaa
     4321 caaatagaaa atggagaaaa ctatctttct atcatggaag caaacctcga agcgttgaaa
     4381 aaaacgacag agacagaaaa cccgctagaa gatactttga cccccaaaaa agaaaagagc
     4441 gtctacaacg gttattttga agacgaggat gtacaagatc gctctctttc agattgggca
     4501 ggaacttggc aaacagtaga tacgtatgta gaagatggga cttttgatcc tgtttttga
//
LOCUS       DBNICI010000186         4429 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.190, whole genome shotgun sequence.
ACCESSION   DBNICI010000186 DBNICI010000000
VERSION     DBNICI010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.190"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(543..2048)
                     /locus_tag="AC1ZCC_002574"
     CDS             complement(543..2048)
                     /locus_tag="AC1ZCC_002574"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HIQ1630789.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="AC1ZCC_002575"
     CDS             complement(2045..2731)
                     /locus_tag="AC1ZCC_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIQ1630790.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="AC1ZCC_002576"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="AC1ZCC_002576"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HIQ1630791.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1238 a          919 c          729 g         1543 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaaca
//
LOCUS       DBNICI010000187         4389 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.191, whole genome shotgun sequence.
ACCESSION   DBNICI010000187 DBNICI010000000
VERSION     DBNICI010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.191"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            205..435
                     /locus_tag="AC1ZCC_002577"
     CDS             205..435
                     /locus_tag="AC1ZCC_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HIQ1630792.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            532..1833
                     /gene="murA"
                     /locus_tag="AC1ZCC_002578"
     CDS             532..1833
                     /gene="murA"
                     /locus_tag="AC1ZCC_002578"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIQ1630793.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1846..2022
                     /locus_tag="AC1ZCC_002579"
     CDS             1846..2022
                     /locus_tag="AC1ZCC_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIQ1630794.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2157..2453
                     /gene="yidD"
                     /locus_tag="AC1ZCC_002580"
     CDS             2157..2453
                     /gene="yidD"
                     /locus_tag="AC1ZCC_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIQ1630795.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2517..2984
                     /locus_tag="AC1ZCC_002581"
     CDS             2517..2984
                     /locus_tag="AC1ZCC_002581"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1630796.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3069..3953
                     /locus_tag="AC1ZCC_002582"
     CDS             3069..3953
                     /locus_tag="AC1ZCC_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIQ1630797.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            4067..4303
                     /locus_tag="AC1ZCC_002583"
     CDS             4067..4303
                     /locus_tag="AC1ZCC_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HIQ1630798.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
BASE COUNT         1492 a          671 c          958 g         1268 t
ORIGIN      
        1 tgattgaaca ccgtttattt ggaataaggg actgcgacaa gacttttgtc tcagtccctt
       61 tttttgcata tttttagcat ttatttccta aaaaatgtaa ggggattcct atatttttaa
      121 gtatacttgt ttttcctttt atacatttta tggtattatc attttattag ttattataaa
      181 acaatcgtta gaaaggacgt cattatgcaa gtatacggaa ttgatgcggt gcttcgaatc
      241 gtgagtcatt tggcgtttat ctacttagcc ttctggtctt tacgttcgtt acgcattgaa
      301 acttttttca gaccaatgca taatacgcaa attagaatgg caatcgtact tttttcaatt
      361 tttttagggt acactgcaag taattttttc ttagaggtta tcgctttatg ccgaaatctt
      421 ttcgttagcc tgtaaatttt aaaaaaaggt actattttaa aagcaaggat gttataatag
      481 aaaagatttt gagtaagtgt atgctcattg gaattttttg gagggaacaa catggaagag
      541 atcatcgtaa gaggtggcaa tcaattaaat ggaacagtaa gaattgaagg agctaaaaat
      601 gctgttttgc ctattttagc tgctagtttg ttagcagaag aaggtattac gacattagac
      661 aacgttccga ttctttctga tgtcttcact atgaatcaag tgattcgtca cttaaacgta
      721 gatgtagatt ttgatgaaca aaagaatcaa gtaacaatcg acgcttcaag acaattagaa
      781 atcgaagcgc cttatgagta tgttagtcaa atgcgtgctt caatcgttgt gatgggtccg
      841 ctacttgcca gaaatggaca tgcaaaagta gctatgcctg gtggatgtgc gattggtaaa
      901 cgaccaatcg atttgcattt aaaaggattt caagctttag gagcaaaaat tattcaaaaa
      961 aacggctata tcgaagcaat tgcggatgaa ttgatcggta acacgattta tttagatttc
     1021 cctagtgtag gtgctactca aaatatcatg atggctgccg taaaagcaaa aggaacaacc
     1081 atcattgaaa acgtagctcg cgaaccagaa atcgttgatt tagcaaacat tttgaataaa
     1141 atgggcgctc aagtctatgg agctggtact gaaacaatgc gtattgaagg cgtggatcat
     1201 ttacatgcag tcaaccactc aatcgtacaa gatcgtatcg aagcaggtac ctttatggtt
     1261 gctgctgcaa tgacacaagg caatgtgctg attgcagatg ctatctctga acataaccgc
     1321 ccattgattt ctaaattgat cgaaatgggt gcagaaatca ttgaagaaga aggcggagtc
     1381 cgtgtcatcg gaccaaaaca tatcttacct acagatgtaa aaacgatgcc tcatcctggt
     1441 ttccctacag atatgcaagc tcaaatgaca gctatccaat tagttgcaga aggtacaagt
     1501 gtggtgactg aaacagtgtt tgaaaatcgt ttccaacatt tagaagagat gcgccgtatg
     1561 aacgctcatg tcaaaatcga cggaaatgta gcaatcatgg atggaaacca tgaattgcaa
     1621 ggtgcagaag tatatgcaac agacttgcgg gcagcagcag cattagtttt agctggcttg
     1681 aaagcaaatg gaatcacacg tgtgcgtaac ttgaattatc tagaccgtgg atattataat
     1741 ttccatatca aactacagca attaggtgca gacgtcgaac gtgtagacat ggatcaaact
     1801 tctgctgaaa aaacagcaca aacgattgct taaggaagtg gctgaatgag taaccaacga
     1861 tatatcttat taactttgat aaagatttta gtggtgatct tattgcttat ccttcttttt
     1921 gtggccggaa cgatgatcgg atatggtgtc atcggtggcg gcaatccatt taaagtattc
     1981 cagccaagtt tgtggattca tattcgagac ttttttcatt gataaataag aatcacagtg
     2041 gttctagtcg atatgaagtg agccatgaag cagaacatga taaaagaaaa cagaagggaa
     2101 cgttgacgtt agtcgcgatc ccttttttta gtaaaagaaa agaaggaatc taattgatga
     2161 agaagatatt gatttattta gttagaggat atcagcgctt tatttcgcct gcatttccac
     2221 ctagctgccg ttattaccca acctgttcca attatatgat aaaagcaatt caaacccatg
     2281 gtgcgctaaa agggacggta atggggactg cacggatctt acgctgtcat ccttttgtca
     2341 aaggcggtat cgattatgta ccattgacat tccgattaac gcgaaataag aaagataaaa
     2401 aagaaccaga ttataaaaag gttgtgaaaa atacgggtag cactgagcga taaagaaaaa
     2461 gaggaaattt attgtcaaag taacaactcc gcaactactt atatggggga caaaaaatga
     2521 tcgaagaact aaacaagaaa gatttaccat ggtccttgtt attagatgca gatcctgata
     2581 aagaaaaagt tcaagtatat gtttccagag ggagtggctg gatatggaaa gaaaaagaaa
     2641 aaacaatagg tgtactgatc tatgtggcaa gggaaaaaga atacgaaatc gtcaatgtag
     2701 cagtaacccc aacgtatcaa ggaaagggga ttggcggaaa attacttgag acagcttttc
     2761 agaagctttc acagctagct tcaaaccaaa ctcggatcat tatacgtact ggcactacta
     2821 gtagcgctgc gttacacctc tatcaaaaaa tgggctatgt ggagattggt cgagtcaaag
     2881 actattttat ccatcattac tctgagccca tttttgaaaa tggagatcaa ttaagggatc
     2941 aagtgatttt agcaattcac ttacctaaaa atagctttaa ctaataaact ttcaattcga
     3001 gaaaatgctt tcaaatgcta aatagataca ttacaatagc tttagcaaac aaaaaggaga
     3061 atatttttat gggaatatta gtttttgatt ttggtggatc ggctgtcaaa tatggttgtt
     3121 gggatggcaa agagattaaa ggtaagggac aatttgcaac gcctgaatct tgggaagaaa
     3181 tgaaaacgca attatttcaa gtttataaaa aaatactcgt ttcttttgaa gtcgaaggaa
     3241 ttggaatcag ctctcctgga gtagtgaata atgagaaaca ggtgattgag ggaatcagtg
     3301 cgattcctta tattcatgga tttaatattt tccgagattt ggaaactctg tttcaactcc
     3361 cagtaacgat tgaaaatgac gccaactgtg ccggaatggc tgaattttat gaaggtgctg
     3421 cccgtgatta tcaagatgtt gcttttgttg tggcaggaac tgggattggc ggggcgttgt
     3481 tcacgaaagg acaaataaat aaaggggcac atctttacgg tggagaattt gggttgatgt
     3541 tcttagatgg cgataagaca ttcagtaaac ttggtacagc ggtacagatg gcttggcgtt
     3601 attgtgaacg caaaggtctc gataaaaatg cttataccgg aaaagatgtc tttgaattag
     3661 cagaaactgg agatgcaata gcaaaagaag aagttgagag tttttatacg tatcttacaa
     3721 agggattatt cagtattcaa ttttcgtttg atccagaagt gatcgtatta ggcggcgggg
     3781 tttctgcaaa agaagggctc attgatgaga tcaaaagccg aatgaagaag ctgacagaac
     3841 aattcgatct gcatgatttc gaaccgcaga ttttattatg tgagtacaga aacgatgcta
     3901 atcttatagg tgcagcagca aactatctcg ctgtacgtca aggagagaat taagagcttt
     3961 agagaaggtg cagcttgcat aaaagaagag aatgaaatga ataggagatt tttacctaag
     4021 gaatgtttta tttgctgcag tatgataaaa tatctgagag gtgaaaatga tgtcaaaaaa
     4081 aaataaagat attgaagtgc gtatagaaga agtgaaaaaa gatatcaaag gtaagaatta
     4141 tgaagtcact cagttattta tagggaaaaa aatgattggt gagatcttag cctatgggcc
     4201 aaaagaatat gaaattttct ttggtgaaga agattttgga aaagaaaaaa gtcttgaaaa
     4261 tgcgatagaa acagtgattc gacactggaa tctgcacgaa taaaaaaata tcaaaaaaac
     4321 tttcaataaa gggttgcaaa aatctggaaa attaggtatt ataactaagt caggttgata
     4381 caaaccttt
//
LOCUS       DBNICI010000188         4319 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.192, whole genome shotgun sequence.
ACCESSION   DBNICI010000188 DBNICI010000000
VERSION     DBNICI010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.192"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..538)
                     /gene="sgrA"
                     /locus_tag="AC1ZCC_002584"
     CDS             complement(<1..538)
                     /gene="sgrA"
                     /locus_tag="AC1ZCC_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HIQ1630799.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTE"
     gene            complement(734..1573)
                     /locus_tag="AC1ZCC_002585"
     CDS             complement(734..1573)
                     /locus_tag="AC1ZCC_002585"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIQ1630800.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            1758..2645
                     /locus_tag="AC1ZCC_002586"
     CDS             1758..2645
                     /locus_tag="AC1ZCC_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIQ1630801.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(3239..3933)
                     /locus_tag="AC1ZCC_002587"
                     /pseudo
     CDS             complement(3239..3933)
                     /locus_tag="AC1ZCC_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(3917..4315)
                     /locus_tag="AC1ZCC_002588"
     CDS             complement(3917..4315)
                     /locus_tag="AC1ZCC_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HIQ1630802.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1430 a          853 c          641 g         1395 t
ORIGIN      
        1 tttctgtgct aatttcactg ctcgtactct ctgtactgct ttcaccgctc gtgttttctg
       61 tactgctttc actacttgtg ctatccgtac tgcttggcgg tccaacaact gtccaactgc
      121 ttggtttcaa attctccgtg atcgtcatct gtccatcata aaaaacaatc aaatcaactt
      181 tatctttact cttgatctca tcaatcgata gttctgcaga aatcattatt gtatactcaa
      241 taccagcggt ccattgtacg tcttttaaat aaacttcccc aataactttt tctgaacttc
      301 cattaccatc ccaaccatat gttagaacag catgtacatc actagcattc acgcttttgt
      361 ttggtttgac tggaatagta atgacttgtt gtgtcatgtc ataaacaatc cgatcagttt
      421 tcacagtaaa tgaaagattc tcatttgccc gttcatttgc agaagtactg attccaatca
      481 ttgctgcaat tatactcagc ccaagaataa caaataatac ggtcgctgtt ttcttcattt
      541 ctatcactct ccatataatt accttctatt tgataaagga attatataaa atgaaaatga
      601 tgagaacacg acagtcagtt gtgaaaatct aacaaaagat gtcgtgtatt cgcttgaatt
      661 tgtttacttt ttcgtttaat caatgacaga cttaaaaaag caagaccgat atcagccttg
      721 ctttcttcat cttttattga taaacagcag tgtatttctt catccgcttt gttttatatg
      781 catagagtaa caaaccaaca atcaagacta acaagaataa tgtagttgat atagatgctt
      841 cagccccaaa gtttccccca gtaattattg tattttgcga ttggatcaga gtggagaaaa
      901 aaccttgttc ttgcgttccg ctgactggta tacccaaaat agctccttgt gtccaattcc
      961 aagtaatatg agcaccaata gccgtaaata aacttcctgt actgtagata agtgcgctga
     1021 aaaagcaacc tgctaaaaag atattgaaaa ctgccagaaa tgtcatgtta ggattcaatc
     1081 cgtgcaaaag agcaaataat gcagcagatc caatcattgc tattaacgga ccaaatctaa
     1141 gcatctgatg ctgaaaaaag ccacggaata aaagctcttc tgatgccccc actgcaataa
     1201 ataaaataaa actcgttatc agcggcacaa tgtttgcgtt tcctgaatct aaccgatacg
     1261 cccccgtcaa atataatgga ctagtaataa gcagaaacac tccaattcca acaacgagac
     1321 ctcccgctaa atacccccag tcttttcctc gaacagaaac accataatct tctattcttt
     1381 ttccttcgac cttactagca agaaataaag ctagtaaaaa aacagctaaa tgtccgaccg
     1441 taccggcaat tgctccaatc cattcactat ttccaataaa catatccgca agtgcattat
     1501 tgacaagaat tcctaaaaca aaaccaacaa agactattaa aacagatcca atcaaccgaa
     1561 taatcctgat catctttcat ccctccttta tattaagttt agcaaccctt ttctttcttg
     1621 tctcgtgaaa actatgtaaa tgttctccat catagagaga atagattttt acgaactaag
     1681 aatatctaca gcaaccttac gaaaatgaaa gactgtctcc ctaattcttc tgttagactg
     1741 tttttaataa ggaaaggatg ggaaattttt tgaaaaaaca actactaatt atgtgccttt
     1801 cagcatgtgc aattttacta ttctcaggtt gtagcaagtc caatgaaacg attgcaacgt
     1861 ttaaaggtgg aacacttaca gttcaggatt tttatgataa cccaaaagtt aaatcaaata
     1921 atcaaacact attaaaaaaa atgattgtct tcaaagcatt tgatgatgta tacggaaaag
     1981 agatttcttc aaaggaagta acaaaagaat ataatgaaca aatcaaaaaa ctcggtccaa
     2041 actacaagga acaattaaaa gcagttggcc aaacagaaga gacatataag aaactcttta
     2101 aacagatgct ggctttccaa tacggtttga aagccaatgt aaaattaaca gataaagatc
     2161 tagacactgc ttggaaagaa ttctatccag aagttagtac acagatcatt ctattttcga
     2221 ctaaagaaga agcagataaa gcaaaaaaag aagcaaatga aggagaaaat ttctccaagc
     2281 ttgttcaagc ttatggaaaa aataaaacat taaaggaaac tgatggaaaa atgaattttg
     2341 attcaaccaa tccagagatt cctactgaag taaaaaaagc agcatttaaa ctaaaaaacg
     2401 gggaagtttc tgatatcatt cctgtaacag atccaacgac ttatcagcaa agctattatc
     2461 tagtaaaaat ggtaaaaaaa caggataaag gttctaataa agacaaatac aaatctgagc
     2521 tagaaaaaat tgctaccgaa gcaaaaactc ttgatacgga attcatggat aaaacaatcc
     2581 gtaaagtgat gaaaaaagac aatgtaacta ttaaagatcc ttatgtaaaa aatatattca
     2641 aataaacaaa tttgacccaa ttggggatgt ggcaaaattt gagaagtgat ttttggaaat
     2701 tccttcttat ttttgaggca aaccactttt gccacaactt catcggggcc tttttttatt
     2761 tgtaccaaaa caaatcactg gatttgttac aatcatgagg atataattta aaaaagtttt
     2821 ttatttctat cacatgaatc aatgcgccat taagtagatt ctttcatgct tccctatttt
     2881 ttcacttgcc gtatcgcttc aaaaacaact atcataaact caaactcgac tgaaaagtta
     2941 ttttaaaaaa gactaaaaat atcccttctg gacttgttct tcttattctg ttatactaga
     3001 gaaacacgaa agatattttt atgcgacact tttcatttgt ttttatgaaa tttattcaaa
     3061 actggcgtaa cttcagagtt acaaggatgt attagaggtt agggaataca tcgtttaaat
     3121 gagaaataag taatagttag taatagtcac ccaaacttta aattaatgaa tattgaatcg
     3181 tcagatagcc tcctcgagtt ttcgagaagg ctatcgttta ttttatggag aaattgattc
     3241 agaagtaatc agcgaccttt tttagttcac caatcgtttc tgcttggaaa gtttgcaccc
     3301 aatcttcttg gaagcccctc actgctttat caatcgaggg catctctaat acttgatcta
     3361 gtagctcttt acctaacgtt atcgctgagc tccagatcga atggctgtag tgacttgttc
     3421 acaatttttg aaactagctg ctaatatttt tgttggtctt tgcgtacgtt cgatctcagt
     3481 ccgcaacgaa tcaatcactt tcatagcatc aatgccttga ttttgcatcc tgttataata
     3541 aggtgcaata taatctgctc cattattaat agctaaatac ccttgtaatt ctgtgtaaat
     3601 agctgtaccg gtaatcaaat aaccttcttt tttcaacttt ttgatcgctt gaaggcctgc
     3661 ttgattcacc ggcaccttga taaacgtatc ttttcccagt tcttctatga taactcgagc
     3721 atcctctatc atttcttctt ccgttcttcc tattgtctga atatggatag gaatctgtcc
     3781 gattattttt ttgatttctt tcatatgcga gaagaagttt gacgctcctt cttttttcag
     3841 aattgtagga tttgttgtta cgcctgttaa attcaaaaac tgttgacaag aataaatact
     3901 ttttgtattt gctgtatcaa gcatgatttc catatagctt tcccccattc tgatcctctt
     3961 gatcgatttt aatgattgtc ccctttctta aatgcggcat ttccttatct tctacataca
     4021 ttgttcctgg aagttccggc tcatgagatc catcaaattt tatcgtgata tgtcctaatt
     4081 cattcaaatt agtttggaca acatctccta ctgctgtgat tacatacatt tgtttatcaa
     4141 aacagagctt catatcacaa gttatttctt ttcttacctg attcaattca atcttataac
     4201 aataatctgt taatgtttcc ggtgcttcat ctccaaataa aatcattatt ttttctttcc
     4261 tgaatacttc tgcttctata ccaacatctg ttacaaccgt ttcaaagatc gtcatctct
//
LOCUS       DBNICI010000189         4244 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.193, whole genome shotgun sequence.
ACCESSION   DBNICI010000189 DBNICI010000000
VERSION     DBNICI010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.193"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..481)
                     /locus_tag="AC1ZCC_002589"
     CDS             complement(<1..481)
                     /locus_tag="AC1ZCC_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630803.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMKVLLRSLKIKRALSEL
                     "
     gene            complement(1040..1357)
                     /locus_tag="AC1ZCC_002590"
     CDS             complement(1040..1357)
                     /locus_tag="AC1ZCC_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIQ1630804.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(1358..1612)
                     /locus_tag="AC1ZCC_002591"
     CDS             complement(1358..1612)
                     /locus_tag="AC1ZCC_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIQ1630805.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            complement(1872..2096)
                     /locus_tag="AC1ZCC_002592"
                     /pseudo
     CDS             complement(1872..2096)
                     /locus_tag="AC1ZCC_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2650..2736)
                     /locus_tag="AC1ZCC_002593"
                     /pseudo
     CDS             complement(2650..2736)
                     /locus_tag="AC1ZCC_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            complement(3315..>4244)
                     /locus_tag="AC1ZCC_002594"
     CDS             complement(3315..>4244)
                     /locus_tag="AC1ZCC_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIQ1630806.1"
                     /translation="AKQNEQRANVKKSTMKQREQLMRLLKEDKLGARSIDTIKPSDAK
                     EWALRMKEKGFSYNTINNHKRSLKASFYIAIQDDCVRKNPFDFKLSEVLENDTKEKVA
                     LTEEQEQALLSFIKTDNVYHKYYDDVLILLKTGLRISELCGLTVADIDFKNEVVIIDH
                     QLLKSKEQGYYIETPKTKNGTRQVPLSRETIQAFQRVMKKRPKAEPFVIDGRSNFLFV
                     NHKGKPKVAIDYNALFVRMVKKYNKQHKDNPLPHITPHTLRHTFCTRLASKNMNPKDL
                     QYIMGHSNISITMNWYAHASIDTAKSEVQRLIA"
BASE COUNT         1300 a          784 c          648 g         1512 t
ORIGIN      
        1 ggagttcaga gagggcgcgt ttgattttca aacttcgcaa cagaaccttc atatttcctt
       61 gattacctga ggcaatgaaa gacttatcta cttgaattag ggtgaaattc tcattcaaat
      121 atggtagtaa aattaatata tgtgaaaatc gaatcttgag atagctattc cccttgagat
      181 attcactgat acttctactc aaatcattta aatttttata attatttgta cggaacgtat
      241 atatatcaac atcagttttc tttacatgag aacttattct aaaatgaaaa agcctactct
      301 cagaaagaat tgaaaaatag atactttcag tagatactga agattgaaca aaattaaact
      361 tgtatccttc aggcatagca cttttaacag tctgctgcat taactcggat aatcttttga
      421 aagtagtaat atatttttta ccagcaaacc atacatatct tttttcatta agtatgttca
      481 ctatctctcc tccattaatt atatttagca tcaaatagtt attgtaccta tattttatcc
      541 cattcctttt aaaacaagaa tacgtacaaa gtaaaatgtt gtaaatagtc tagcagggat
      601 gtatattttc atgtaccaac ataatattta agaagtttcc catagatata ttggacttcc
      661 tcttttaaaa cacaaaaata aatactacct tatttaggta gtatttatta aaagttacgt
      721 caattttgat taaaaatata ctacttgaac ctctcatgag taaagtcacg aaatttttgg
      781 gaatagagcg tacttgatat cttatttttt cactaatagc agtgtctctt attcaccaag
      841 cgatccccgt agttcctacg gttcttaatt tttctctaga ctaatgattg tatctttaga
      901 ccttcggcca taacatggag caaaataaaa aggtactaag tcttatatat caatcttcga
      961 gtaaccgacg gttaacaacc atatcgagcg gcattaatca cccacctgta gaggatgagc
     1021 gacttttgcc taattattgt taaaagccta gtttcatttg tttagattgg aaaatacgtc
     1081 ctacaagtat ctcttttttt tcatggtcga tccgataaaa taaggcatag cttttgccga
     1141 tcacgatccg atatgttggg atatccattc catagatttc ggaaacttcc tcatgcatag
     1201 ttggaaaatt tttggttagt tctaaagcct tctggatcgc acctacaaat ctttttatgc
     1261 tggctggaga aagagcaagc tcattttccc attcttctat tatcgtttca aggctggtct
     1321 ggaaactttt agaataaact agcgtgtact ctttcatcta ttcactccag ccgtattttc
     1381 cgaaagcttt taccatttca atttctgaaa ctgttaaacc tttctcaaag tcttggaaac
     1441 tttcttcaat caatttgcga tcgtgttctt ttgctatttg agtagtatct aatatgaata
     1501 agttgccttc ttgtttggct tggatagtgt ctccgctttt taatccaaga gagcttggaa
     1561 ggatcactcc cacgctattg ccgatctttc taacctttag ttcttttccc atgttatcac
     1621 acttctttta gtttttgata aataaattat aacatgttat aacgacaatt gccaacggtg
     1681 tttcttaagg gattccactc catattatta ttggacaaaa taagccactt agaacgctga
     1741 aaacatatac aaacgatatc aaaatacgag tgaactactc accatacact tccgttaaca
     1801 tccatttaag taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga
     1861 tatagctaaa gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg
     1921 ttctcggcta attcataaaa attgttatgt gttccatata gcaatactgg aataacttta
     1981 ttttcttgaa tctcataagc aactctatac acggtgaccg catacttaaa tccattagac
     2041 caattacctg ataaacctcc agttttttgc tcttacttaa atggatcaat agcaatttca
     2101 tcacaaacct atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta
     2161 actctatgta gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc
     2221 taaaaatata tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa
     2281 aatcaataca taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa
     2341 tagttttgct gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa
     2401 tacctttttg attttgtttt tgcaatcaag ccatttgcag aggtaactgc acactacatc
     2461 tgcaagtggc ttgattgcaa aattgccaaa aaagacagta agcctaccag ttaattctat
     2521 aatatagaaa cgtaaaaaaa accccatcag cctacacgct gatggggttt gatgatataa
     2581 tgaaatacct ctttgttact cataattata ccataattag tcacgaatgc aaccaataga
     2641 aaaaactact tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt
     2701 ttgtgaagca ttaaatacat tgttattaat tgtcgctgtt tttaactagg cctctcccct
     2761 tctattataa aagaagatat atattatttc ttaattacct actaccttaa accaaaattt
     2821 aagtactacc taatttaagt agtacttaaa taatctaaaa aatagttatc ccaattgtta
     2881 tgcttttata atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata
     2941 tttaggtagt gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca
     3001 actaagttat gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta
     3061 caagcatgta gttttatctg ttaattatgt actacctaaa taaagtagtt tataattaac
     3121 aggtatatat atttaaaaat ttattataaa ttatatatgc ctttttgact atctttttat
     3181 atcttctcaa atgtccgcaa agccttattt tatcggctct acgggcattt tgcttttgtg
     3241 gtaaacctca catatctagg tctatctttt tatattttcg ctatcaaacg tggttaaaat
     3301 cgtggtaaat acttctatgc gattagacgc tgaacctctg attttgcggt atctatggac
     3361 gcatgagcat accagttcat tgtgatactg atgtttgaat gtcccatgat atactgtaaa
     3421 tctttcgggt tcatgttctt gcttgccagc cttgtgcaga acgtatggcg tagcgtatgc
     3481 ggtgtgatat gtggcaaggg attgtccttg tgttgcttgt tgtatttctt taccatacgg
     3541 acaaataagg cattgtaatc aatcgcaact ttgggcttgc ctttatgatt gacaaacaga
     3601 aaattgctcc gtccgtctat cacaaatggt tctgcctttg ggcgtttttt cataacccgt
     3661 tgaaatgcct gtattgtttc tctgcttaat ggcacttgcc ttgttccgtt ctttgtttta
     3721 ggcgtttcaa tataatagcc ctgttccttg ctctttaata actggtggtc gataatcaca
     3781 acttcattct tgaaatcaat atcggctact gtcagtccgc acagttccga gatacgaagt
     3841 ccagtcttta acagtatcag cacatcatca taatacttat gatacacgtt gtccgtctta
     3901 ataaatgaca gtagggcttg ttcttgttcc tctgtcaatg ccactttctc tttggtatca
     3961 ttttctagga cttcacttaa cttgaaatca aaagggttct tccttacaca atcgtcttgt
     4021 atggcgatat agaatgacgc ttttaacgag cgtttatggt tgttaatagt attgtaggaa
     4081 aagcctttct ctttcatgcg taacgcccat tccttagcgt cagagggttt tatcgtatca
     4141 atgctcctag cacccaactt gtcctctttc aataaccgca tgagctgttc ccgttgtttc
     4201 attgtgcttt tcttcacgtt tgccctctgt tcgttctgtt tggc
//
LOCUS       DBNICI010000190         4224 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.194, whole genome shotgun sequence.
ACCESSION   DBNICI010000190 DBNICI010000000
VERSION     DBNICI010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.194"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(77..607)
                     /locus_tag="AC1ZCC_002595"
     CDS             complement(77..607)
                     /locus_tag="AC1ZCC_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HIQ1630807.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            712..1263
                     /locus_tag="AC1ZCC_002596"
     CDS             712..1263
                     /locus_tag="AC1ZCC_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIQ1630808.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(1416..1889)
                     /locus_tag="AC1ZCC_002597"
     CDS             complement(1416..1889)
                     /locus_tag="AC1ZCC_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIQ1630809.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            2027..3499
                     /locus_tag="AC1ZCC_002598"
     CDS             2027..3499
                     /locus_tag="AC1ZCC_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HIQ1630810.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            3496..4206
                     /locus_tag="AC1ZCC_002599"
     CDS             3496..4206
                     /locus_tag="AC1ZCC_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HIQ1630811.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         1323 a          758 c          819 g         1324 t
ORIGIN      
        1 atgaggaact atttttccgt ttctttctct aatactcagg tcagaacgct gatttcacaa
       61 cctctctttc acaaccttat tttactcgtt tataattttc atcgaagtat tttccgctac
      121 ggatatcatc tggcattcca tcgtatggtt cttcgctaat aacatcttca ttgtctatac
      181 ctgcatcacc catataagtg atcgtagcaa attcaggaac aagtaatttt ggataagttt
      241 catatttttc acgagctgct tccatttctg cgatatgttc gttttggaaa gtaacagtga
      301 tttctggatg ttcttcaacc catttaggga aaaagatcat tccgtgaagt ttcttttcat
      361 ttggcataaa gtccaatgcc acgtctgtcc ctgttggctc tgtccaagtg attttgaata
      421 cgccgtccgt caatttaacg atatcagctt cttggtcttt tacccaacgg cctttgacca
      481 ttccgccatg gatacggtaa tcaacagtat ggtcattttt ggcataccat tcatattccc
      541 aaccattgtc atacgtatag ataaagtgag ttccaataaa atcttccaat tctttaaatt
      601 ctttcatgtt taagcatctc cttataaatt aaaacatgta ataaactaca tgttttcgat
      661 tactcattta tgatagaatg gatttaaaga tttgtcaagg agggaaaact gatggcaaga
      721 aaaaatacac ttcgatatat tttgttaggg ttattaagta agaagaagat gacaggatat
      781 gaattgaatc aatcgttcaa aaatgaaata ggagaatttt ggcaagcaaa acatagtcag
      841 atttatcctg aacttgcaaa aatggaagag caaggaatca ttcagcatca agtagagatc
      901 acaggtgaaa aattggaaaa gaaagtttat gaattaacag aagaagggaa ggaacaattg
      961 gcagagtgga ttcatacacc caccaatgaa ctaccggtta atcgagacga atttgttctg
     1021 aaattgtatt tcgtcaaaga cgtgaatgat ccggcattag tagaaattat tcagcagcag
     1081 cgtgaccttc atgaagaaaa acgacttcat ctattagagc gaaaaaaaac gatctttcct
     1141 actgaaaaag aaaaacatga aaattacgga cactacctga ttttagcgca tgcgatcaat
     1201 cgggaaacag aatatgcaaa ttggctaaca gatgtattag aagaaagtaa ccgaaaaaaa
     1261 taaaaaaata tccgaacagt gatggagaat tcctttctat atgaaagaaa cgcagatcac
     1321 cgttcggata ttcttatgtt caaacaggcc ttttttcaag tatttatgta gcatctactt
     1381 attaaaaagc ttggtactta ggagattagt ggcgtttaga gttcttttaa caactgttcg
     1441 tagtcagctt tttcatcgac aggaaggagc ttgtaagtta attcgtctgt ctcgttgtca
     1501 tgaatgatgt acatcttagc attctcttct ttgaagttac cgatttgtaa aatcgtatag
     1561 cgttcgtcga tcaattcgat atctttgatt gcttttgaaa aaactttacg gacatggtgc
     1621 attttctttt ggcggatttc tgccttttct gcagcttttg acaaagtaaa tccgtcatct
     1681 aaatattttt tgatcagttc tacttttatg actgtaggta aacgatattt tctaggacaa
     1741 ttcgcatcag cttcgatcga acgaatgaat cccttttgtt cccaatacct taattgacgt
     1801 ggagacacac ctacgatatc acttaattca ctgatgccaa caaccaattg atcattttcc
     1861 aacaaacgtt taagggaagc cccaaccatt tttgtcactc cttttgtcct cttgcttccc
     1921 ttaattatat ccgtattaac ttatgtgtca agtaggttga caaatagcga atttaggact
     1981 aaagtattct agtagtttca gaaaaaattc agaaagaagg agaaatatga gtaacaacca
     2041 acccgtagat atttacggaa aaccatataa tcggacactt ttagttgttg tcttattaat
     2101 tggtacattt tgtaccgttt tgaatcagac cttattgacg acagcttttc ctacattgat
     2161 gaaagctttc gatatttcag cgtctgatgt gcaatggtta acgactggtt tcttgctagt
     2221 caacgggatc atgatcccaa taacagcatg gctgatcaat aaattcagtt caagaaattt
     2281 atatctttct gccatgacga tctttttgat cggaacgatt acttgcttta cagcacctaa
     2341 ttttagtaca ttactaatcg gtcgtttgat ccaagcttgc ggtgtgggag tatcgatgcc
     2401 attattgcaa aatatcatgc tatctatttt cccgccagaa aaaagaggat cagcaatggg
     2461 gatgtcaggg atcgttattg gacttgctcc tgctttaggc cctacattat ctggttatat
     2521 cattgataac taccattggc gtgacttgtt tggtatggta ctgccaatcg ttgttcttgt
     2581 cttggtttta gcattcttcc taatgaaaag tgttattcca ttgtcaaatc ctagcattga
     2641 tattttatcc gctattttat caacgattgg attcggtagt ttactttacg gattttctag
     2701 tgtaggagat gacggctggg gaagcttgaa agtaattggt ttcttagttg ttggtgtgat
     2761 tttcatcgcc ttgttctcat ggcgtcaatt acatttggaa catccattct tggaacttcg
     2821 tgtattcaaa tcatctacat tcacgattgc agctgtttta gctggtgtaa caaatatggc
     2881 gatggtaggg gcagaaatgg tcgtaccatt gtatattcaa aatattcgtg gagaatcagc
     2941 attccattct ggcttgatgt tgttgccggg tgcattgatc atgggggcta tgatgcctgt
     3001 gacgggtgcg attttcgata aatacggcgc gaaacgttta gccatcacag ggatgttcat
     3061 tttgacagcg gcaactgcac catttgcttt cttgacaaaa acgactccag ttttatatat
     3121 cgtgatcctt tatgcgatcc gtatgtttgg gatctccatg gtcatgatgc cggtaacgac
     3181 ttctggtatg aatgcattgc caaataactt gatcagtcat gggacagcgg taaataatac
     3241 attccgtcaa atcgcaagct cgattggtac agctattttg atcagtgtgt tgacgaatgt
     3301 gacaaaagca aatatcccaa gcaaatctgt attggataat acgccattag cttataaaga
     3361 tcaggcaatc aatgccacac tgtcaggtta ccatgccgcg tttatcgtag cgatcatctt
     3421 tgggattctt ggctttatta ttgccttttt attgaagaaa aaagaacctg ttctaatgaa
     3481 agcgggggat aatgcatgat tattggagtt ttgattgtca gcttgcttgc ttttgcttta
     3541 acgaacgtgt tcgctaaaaa aacatggcaa acctttttat ctttgatttt cggactaatc
     3601 ttcgtcgctt cactaagttt gatcgtcgct aatttgtcca atcactttgg aatggaaaaa
     3661 gtaacggaaa caaaaacaga aaaaattgtt tccagtgctg attcccaagg agcagatatg
     3721 cttttgtata aagctttagg gaatggaaaa gaaaaagtgt atctttaccg aacaaatgaa
     3781 aaacaagaaa aaccaaaagc aactggtacg gataacgaaa caaataaagt agaaaagaca
     3841 gacggcgatg ctgaaaaagt gacgaaaact acttattggg aatacaaaaa cgacatgtat
     3901 aaattctggt tcaatattgc tgataacaat cacgaatatg acagccgagt gaatacattc
     3961 aaaatcccag agacatgggt agaattaagt acggaccaag cagccaagtt agctgaatta
     4021 gtgaagaaac agcaaagtac gatggaaagt gaagcaaaag cttatgttca agacggtatg
     4081 gtaaaagcaa tgactgaaaa tccaaaaatg agcaaagcag agcaagaaca acgtacgaaa
     4141 gccttagctg ctgaattcca acaacaagca ttcgcaaaat tagtgaaaga agcaaaaggc
     4201 gaataatatt ttgaacacca tgta
//
LOCUS       DBNICI010000191         4184 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.195, whole genome shotgun sequence.
ACCESSION   DBNICI010000191 DBNICI010000000
VERSION     DBNICI010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.195"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            725..1324
                     /locus_tag="AC1ZCC_002600"
     CDS             725..1324
                     /locus_tag="AC1ZCC_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIQ1630812.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLRDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFPEQ
                     LQAQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTAVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            1373..2089
                     /locus_tag="AC1ZCC_002601"
     CDS             1373..2089
                     /locus_tag="AC1ZCC_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HIQ1630813.1"
                     /translation="MNEEKQPNFPDKYHLSRKESVYLLKKNIVELVYNAGKFEGLNTT
                     LLQTEGIIKYNRANNVAVDDVLTVVNLKRGFEMLLNDVQEPLLETSKRINRIVAAEDA
                     LFPGEIRTGGVELSTIQGRYVPPMLTEDEVNNQYGEIMNQEISDTEKALRLFLFISKN
                     QIFWNGNVGTALLIANKIMFSNGLGLLSIPENVFMKFNELLSKYCNSNLLVNESNIIS
                     FLYEQCIFGINYLKIIQHTI"
     gene            2103..2882
                     /locus_tag="AC1ZCC_002602"
     CDS             2103..2882
                     /locus_tag="AC1ZCC_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002396205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HIQ1630814.1"
                     /translation="MMTIDEAKQLATYFSVGSAGYILDFTNVTWNIFTKKIVGIEIQN
                     HYGGSKGVSFQKFLLDDQFDDSLKNRLIQSLKGIMDRDKKYESTEMNPIVQLLFEEVF
                     KEDTSTALRKMKFSSEIDLLYIRQLPIRIQTDIKNKDFDSVLTKANTLIDEVLKFIIE
                     NTEGPSVNINLFKSKELRKEVFGRLNIKTEKDMDNRIKALVGALNTLSDKILEMRNSQ
                     GDAHAHGSNRIVIKEEEAILVANSSMVLCEYVFTKYKGLNR"
     gene            2965..3321
                     /locus_tag="AC1ZCC_002603"
     CDS             2965..3321
                     /locus_tag="AC1ZCC_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIQ1630815.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVDNGRYVITHGFTKKTEKTPITE
                     LKHAKELREEWFTNDD"
     gene            3311..3577
                     /locus_tag="AC1ZCC_002604"
     CDS             3311..3577
                     /locus_tag="AC1ZCC_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1630816.1"
                     /translation="MMIKKLIEEKKKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            3736..4014
                     /locus_tag="AC1ZCC_002605"
     CDS             3736..4014
                     /locus_tag="AC1ZCC_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630817.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYVNNSSGQSVINKQIVENRVLLQSFKNKISFCIISSF"
     gene            complement(4011..>4184)
                     /locus_tag="AC1ZCC_002606"
     CDS             complement(4011..>4184)
                     /locus_tag="AC1ZCC_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HIQ1630818.1"
                     /translation="HRSIKRRHKLYQSLRTASTTIKGMETIRGIYKKSRKEGTLFGFS
                     VCTEIRILLGIPA"
BASE COUNT         1543 a          596 c          785 g         1260 t
ORIGIN      
        1 gcatttatta ttcttttgat attggctgct tgcccttcaa ttttatttgg tttcttaatc
       61 aactcttcat ataaccccaa atcatatatt aatgaggtgc ctcctattcc tataaatcta
      121 tctcttcttt aatagtatga gaaaagtcta ctcttgtaga tttaaattgt caacaaaaaa
      181 tttacagatg taaatgataa gttgaataga gaaagataaa ttattttttt ggtattcata
      241 taaggttaat tatgtattat cacaaaatga ttgttgtcga ataactaaaa gtgtcaaaac
      301 aatgatgaaa tcatttagtt cttataaatt agacaagaga aaataaatat tccatatcta
      361 ctttattgga tccataacat gactaaattc tgaatacttt ttaaagctta tcatttggta
      421 aaactgaata taagttaatt atatagactg aaaattttga attttcagaa tagaataact
      481 taaaagtact gacaaatttt ttaaaggggc aatattaatg ttctgagata atcaaatctt
      541 accgaaaata tcaaaagtcc aatgtgacac aattggattt tgtcacattc gttaaaatcg
      601 ctccatcctt agaggctcta ataggaaatc acttttcttt tagcgccttt tttctatagc
      661 ttgtttaaat gtgacttatt atctgttata atcgtattaa gtaacatttg agaggtgtct
      721 aaaaatgaaa agcgtggaac cgattcgcga caaaaaaaag atcgatgcca tgaaggccat
      781 tttggcttcc ggaaaatatg ggcaacgaaa tttggtgctt ttttcgatcg ggatcaatac
      841 agcctatcga atctccgatt taagacagtt aaaattaagg gatgtgctag aaatttcccg
      901 tgggcgggtg atcgtcaaag aacgtctggc catgaaggaa caaaaaacgg ccaaacacaa
      961 ttcggtcttc atttcgaaca aattgcgaaa agtgattctt gattatgttc aatccgaatt
     1021 tcctgagcag ttgcaagcac aagacttcag caagtatttg tttccaagtc gaaaaggagc
     1081 agatacgcct ttgacccgac aaagcctttg gcggatcatt catgaagcag gaactgcagt
     1141 tgggttaaaa gaaattggtc cccattcgat gcgtaagacc tttggctatt ttttatacaa
     1201 gcaaggaacc aaaacagaga tcattcagtc gctgctaaac cattcttcac aacgggagac
     1261 cttacgctat attggaatca cccaggaaga caaagatact gcagtaaaga gtttggattt
     1321 atgacagaat aatgccctat ataatagaag gaaaaattca agaggtgaga aaatgaacga
     1381 agaaaagcag cctaactttc ctgataaata tcacttatct cgcaaagagt ctgtgtattt
     1441 attgaagaaa aacattgtag aactggtgta taatgcgggg aaatttgaag ggttaaatac
     1501 aacgttgtta caaacagaag ggattattaa gtataatcgg gccaacaacg tggctgtaga
     1561 tgatgtcctg accgtcgtta acttaaaaag aggctttgaa atgcttttaa atgacgtaca
     1621 ggagcccctg ttagagacaa gtaagcggat taatcggatc gttgcagcag aggatgcgct
     1681 ttttcccggt gaaatacgaa ccggtggggt agaattatcg acgattcaag gaagatatgt
     1741 gccaccaatg ttaactgaag acgaggtaaa caatcaatat ggtgagataa tgaatcaaga
     1801 gatatcagat acggaaaaag ccctgcgact gtttctattc atttcgaaaa atcaaatatt
     1861 ctggaatggt aatgtgggta cagccttgtt gattgcgaat aagattatgt ttagcaacgg
     1921 tttaggacta ttatcaatac ccgaaaatgt atttatgaaa tttaacgaac ttttatcaaa
     1981 gtattgcaac agtaatctat tagtcaatga gtcaaatatt atatcatttt tatatgaaca
     2041 gtgtatcttt ggtattaatt atttgaaaat aatccaacat acaatttaag aggtataagt
     2101 aaatgatgac tatagacgaa gcaaaacaat tagcaactta tttttcagtt ggttcagcgg
     2161 gatatatttt agactttact aatgtaacgt ggaacatatt tacaaagaaa atcgtaggga
     2221 ttgaaataca aaatcattat gggggctcaa agggagtatc ctttcaaaag tttctgcttg
     2281 acgatcaatt tgatgattcg ttaaagaatc ggttaatcca atctttaaaa ggtattatgg
     2341 atcgagataa aaaatatgaa tctactgaaa tgaatcccat agttcagttg ctttttgagg
     2401 aagtatttaa agaagatact tcaactgcat taagaaaaat gaaattttca agcgagattg
     2461 atttacttta tattcggcag ctacccatta ggattcaaac agacattaaa aacaaggatt
     2521 ttgactctgt tttgacaaaa gcaaatactt taatagatga agttctaaaa tttattattg
     2581 aaaataccga aggaccgagt gttaatatta atttatttaa gtcaaaagaa ttaagaaaag
     2641 aagtatttgg ccgcctcaat attaaaactg aaaaagatat ggataataga atcaaagccc
     2701 ttgttggagc actcaataca ttatcagata aaatactcga aatgagaaat agtcaaggag
     2761 atgctcatgc acatggctca aatagaatag taattaagga agaagaagca attcttgttg
     2821 caaattcttc tatggttttg tgcgagtatg tgtttactaa gtataaagga ttaaatagat
     2881 aataaaatgc atattttata gtatacaatt cgaattttta tgttatatac tataatataa
     2941 aatcaatata tgatataatt gggtatggaa aaaatatctt ttgaaacatt taaaagaccc
     3001 aatggtcatg atgaattttt agaatggttg gaaacattac caaaaaaaga tagtgcgaag
     3061 cttttacgta caatagaaga aacagaaaag aatgggatgc tagtagctca acggttgaaa
     3121 tgggtgaaaa aattagacac taatttgtac gaattaagaa gtaaggtagg atcaaacatc
     3181 caaagagcca tctatttcca tgtggacaat ggaaggtatg tcattactca cggatttact
     3241 aaaaaaacgg agaaaacacc aataactgaa ctcaagcatg caaaagaact tagagaggag
     3301 tggttcacaa atgatgatta aaaaattgat tgaagaaaag aaaaaagata aagattttgc
     3361 agaagcatat gaccgtgaag gaagaaaact cgaaactgcg gttgctcttt atcatgctcg
     3421 ggaggaagca gggctaactc aagcggagct tgcacaaaaa gcacatacaa ctcaatctac
     3481 tattgctcga attgagagag gggataacgt atctttcgat aaacttgatg atatcgccag
     3541 ggcattaggt aaaaagctgg tagtagaatt tgcttaaaag cgagttgaaa atatatctat
     3601 aataggctcc taactaatca attagtttag tgggcctttt tttactagat agataaaggt
     3661 tgtactatag tgtaaaagac aataaatttt atactaaaat tactttaaag agaactagag
     3721 agaaaggtac aataaatgaa aaccataaat cttataaaag tatttatagc atcaccaggt
     3781 gatatagcag caaaaaggaa tgaagtagaa gaaaccatat atagatggaa tatagaaaat
     3841 acagataata caaaagtgat tttgatgcct atacgatggg aaactaatgc gattcctact
     3901 tatgtaaata atagctctgg gcaaagtgtt attaataaac aaattgtaga aaatagggtt
     3961 ctgctgcaaa gttttaaaaa taaaatcagt ttttgtatta tttcttcatt ttaagctgga
     4021 attcccaata atatcctgat ttcagtgcag actgaaaagc cgaagagagt gccttctttt
     4081 cggcttttct tgtagattcc tcgaatggtt tccatccctt taattgtggt ggaagctgta
     4141 cgtagacttt gatacaattt atgtcgacgt tttatcgatc gatg
//
LOCUS       DBNICI010000192         4030 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.196, whole genome shotgun sequence.
ACCESSION   DBNICI010000192 DBNICI010000000
VERSION     DBNICI010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.196"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(238..387)
                     /locus_tag="AC1ZCC_002607"
     CDS             complement(238..387)
                     /locus_tag="AC1ZCC_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630819.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            849..1061
                     /locus_tag="AC1ZCC_002608"
     CDS             849..1061
                     /locus_tag="AC1ZCC_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630820.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1339..3138)
                     /locus_tag="AC1ZCC_002609"
     CDS             complement(1339..3138)
                     /locus_tag="AC1ZCC_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HIQ1630821.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3262..3858)
                     /locus_tag="AC1ZCC_002610"
     CDS             complement(3262..3858)
                     /locus_tag="AC1ZCC_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIQ1630822.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1447 a          711 c          689 g         1183 t
ORIGIN      
        1 taaatattat acagataata acggaatatt atcatcgaaa cagctaacaa aattgtcata
       61 aaaaacgaga tggttgatat tagaacttgt gtttttgttt aggtctagag ataaagagag
      121 acaatttcca aatatatgta atgaatacaa aaagacgttt gtcgcggatt accgttacaa
      181 acgtctttta ttgattttcc gagatctcat cagtcgccat ttggaatggt tcactcctta
      241 tctagattcg tttaatgctc ggactgcttg ttcgattgta ataccaaacg caacgttatt
      301 ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt gtatcaaaag ctacgcggta
      361 ttgcatattt gtttttgtaa acgtcataaa attcatctcc tttgtaatgt aatttagttc
      421 ttgaaagttt cctttaaact ttcgaactta taccaagttt agcatcgtac gttttttgat
      481 gcaattaaaa agcatttatc attttcctta tttatttttt cttaaatggt ttagctattt
      541 tcttttaatc cgataagtag cccaatcatc aaggttacaa atactattgc ttgaatataa
      601 atcatatgct cacctcttta taaataactt agcaatataa agaaaaaaac gcacgaacaa
      661 cataggaata taaacaaacg tttttgtcac aacgtctaag atcgtgttaa aaaatagtta
      721 tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa atggataaaa acgttttcaa
      781 atacaaggta atttgttatt ataaaataaa gaaaaaacaa aataaaaata aagaaaagag
      841 ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat atgcaaaccg atcggagtat
      901 ctcatgaact aatcggctgt attgaacgta catatatcaa cacggctgtc gtgtatgttg
      961 aaagttatga ccaacgagac tatcaactta tcaatgaaag caagtatcgc atgctcgtaa
     1021 aattctgtga tattagcgca cctgctgaat tggtttctta aacagtattt ttgagaattc
     1081 gcaagaactc ccttataata taaataaaga cagaagggag cgagaagata tgccaaaaag
     1141 aaaaagttca gggaaaatgg gaattattct tgcagtgatc tctgcaattc ctgctatttt
     1201 agctattgtt cgtgcgatga aggaatcaag agaaagttaa gcacacatta tgagcgtcaa
     1261 acaaaaaacg catatacctg cggtggcagg ttatgcgttt tttgttgtat aaagactttt
     1321 ttcattaacg atttttgatt agccgactcc caaaacaaaa tttagcagtt tatctgaata
     1381 agtgatttct tcatctgttt gtttcacact ttcatttagt agtcccatct catcggttat
     1441 gataccatcc acaccataaa acatcatgcg actcatagca tctgaatcgt tgattgtcca
     1501 tacatatact tcttttccgt cttggtgggc agaattaatg aagttacggt tgatcgtact
     1561 atattccatt gtcagaaaat cagcaggcgt cacagggggc ccaacaatat tgaaaggtaa
     1621 gatgtagccc acataaaagt ttggttctgc ttcttttaaa tctgaaacgg tttggtacgt
     1681 caaactttgc aaaatatgat ggttctctaa gataactttg cggtatttat ggacaaatcg
     1741 ttcgacaagg tcatccgaat cttttttaga ggtcttgatc tcaataagta atttttggtt
     1801 tagcttatgt gctttttcca aataagaatc aaaagaacaa acaggggctt gcactccgtt
     1861 ttcagtaact gtaagatttg tcaattcttt caatgtaaga tcctgtggtg ttccatttac
     1921 ccctgttaag tgacgaagat ttgcatcatg catcactacg aaatgaccat cttttgtttc
     1981 ctgaacatcc atctctacat aatcaggatg atagtctctg cttgtttttt caagtgcttc
     2041 taaagtattt tgcgccccat tttgggtact cacacctctg tgagagacag tcactggttt
     2101 tgtctgaaca gctgaagtca gataatccat attgtaaaga cagacgccaa taccgaaaaa
     2161 aactgcaaaa agagtcaaac cagtgttttt cagcgcagag aaacgcaagt tgggggcttg
     2221 tgggataaac cacttaggga tctcaggcaa gaagccttcg tcatccatga aatcaacaat
     2281 gatataaaaa atccctacgg tagacatcac gatattgaat aataaaatcc cttgtaaaag
     2341 cgtcatagca aacacagcgc tgatcaatgc atagtctggg aaaaattgtt caaccatact
     2401 ttgtgccaaa ataacaacga tatatcctaa agaagaaagg agtaaaatcg taccgccaat
     2461 cacaataaat tggcccgtga tggccaaaag acgcgatttg gtcaatgacc agctttcccg
     2521 aatggctgct cgaaaaggac gatctcgtag aatcatttcc ggtaaagcaa aaatcagtcg
     2581 aatcccaata tagccaagga aaatataaac gagtaaaaag gaactgacga tgatccaacg
     2641 attagcaaaa ataaaatcca taatgaaagc ggggattttt atttttgaca gtagatcaga
     2701 attaaaactt aatccgctga tcggtagaat caataaaaaa taagcaagga aaaacaaaaa
     2761 agtaatgggt ctcactttct tcagctgaag tatcgtcaag tgtaagagtt gcttcaaaga
     2821 gattggttct ttctttttaa taaaaaaaac actcatcaaa agaaaagtaa attcgaaata
     2881 aactaacagt aaaatcgtca tcaaaactac gattaacgag agtagaaccc caggatgttg
     2941 agaaaaaatt gaaggaatat tgtcataaga caaatagtca attgcccctc ggtgtaaaat
     3001 aaaacgggtc gtactgctca ataagggtaa acaaataaat aaaataaagc catgcatcag
     3061 caagacatca cggaaataag cttgtgttcc ttttaaaaag tcccaagcat tacctaagct
     3121 acgtttcaaa taattcatcc tatcacctgc tcttcacatt gtagcgcaaa tctaatgaaa
     3181 aaggaaaggg tgcagtgtgt tttgcagacc ctttcccaga tttaacattg tcaatcaatg
     3241 actaatttta caaaataaat attatccaag aagaccactt agatattgac ctccataaat
     3301 caaggccata ctatataaga aaatcgacag tggtttagca atcaaaatga ttagtgtata
     3361 tcgtttcact gacatgtttg tcaatccagc catcaaacat aacgcatcgt caggagcaac
     3421 tggaagaaaa atagctagag caaacagctt ctcaaaacgg ttttgattat ccagccagcc
     3481 aatatatttg ttatatgtct tatcgctaat catggaaagt atgaatggct ttccataacg
     3541 acgccctagc agaaaaatga taatcgaacc aatgcagatt ccaatatagt tatagataaa
     3601 tccagcataa gggccaaata tcaggacacc agctgcagta ctgatccctc caggaatgat
     3661 tggaataact acttgcagga tttggatgaa gacaaaaata atcggtccta aaatgatgga
     3721 atcacctact aatcctctta acgcattcaa atctttgaaa acacctaaat taataaaata
     3781 aatcgtcagc gcgattgata ggcctaatcc gataatggat atgaaattta tcagttttcg
     3841 agaggcagca atattcatta ttcatcaact ccttagaggt tctttgttaa ctatacttta
     3901 ctagaaaagc aactaactgc gccatttttt gatatgacag aaaacaaaca attttgtaag
     3961 ttcatcataa gtataaaaga ttgatctcaa aaaaacatcc aactaaagac ttatttaata
     4021 acttgttttt
//
LOCUS       DBNICI010000193         3978 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.197, whole genome shotgun sequence.
ACCESSION   DBNICI010000193 DBNICI010000000
VERSION     DBNICI010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.197"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(18..1484)
                     /locus_tag="AC1ZCC_002611"
     CDS             complement(18..1484)
                     /locus_tag="AC1ZCC_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIQ1630823.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(1775..3859)
                     /locus_tag="AC1ZCC_002612"
     CDS             complement(1775..3859)
                     /locus_tag="AC1ZCC_002612"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140358 - P-type transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIQ1630824.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1202 a          847 c          634 g         1295 t
ORIGIN      
        1 taacaagtat ttctatttta aggctgaacc actgtttctg gttcagtgat ttctttgttt
       61 ttgtctcgac gagcaattgc tggcaagatc atacctaata agatcaagac gattggtgtg
      121 atgatattcg taaataattg gaaccaccat gctttaggat cagcagtata atctactttt
      181 ggcaccattc ctaggataca tgcaaacgca gtgaaggcaa agcaccaagc accaaagata
      241 aaaccgattt ttggattttt aacaaattta tattcagaat tgaatttctt ataagcttta
      301 ttcaacatca tatatgccaa gaacacccat aggtaacgca ttggcatgac aactgagttc
      361 aagttcgtca accattttac taattcattc atatctttga taccaaaaat cggcaacaaa
      421 ataatgattc ctactagtat acctgtcaga agataaccat tcaccaatgt acctttacga
      481 ttacgtttac gtagccagtt tggtacaaat tctggatccg catctgctaa caagattttt
      541 agtggtgcat caattgaaaa ggctaatgcg gagatttgtc caatcatgtt tgtcaatgcg
      601 taaatgatca tcaatgagtt accgatacca tagtaattac ctaaaatttg aaaagcactg
      661 tatgccccat ttgccatcaa gtctttagga atattatcac tagcaaacag catacccatt
      721 gcgactgagc ctaatacagc acaaatccct accattgctg ctaagaagat catcccttta
      781 ggaaattcct ttgcaggatt tcttgtagag ttgacataag gggatatttt ttccgcccct
      841 ccaactgcaa atactagcat cgaaacggtt gtgaaataac tgaaatcaaa tgttggaata
      901 tatgttttca cttgattcat atgtggtgtg gcaaattcca tcgttggatt gatgaacggt
      961 gccgttacag ctaaaagaat aaataagatc gacatgacga acatcgctgt tccggccagt
     1021 ccgccaatca ctttcaaagt agaaagtcct tttgttgata aatataaaaa ggcaagaaat
     1081 ataataagtg aaataactgc taccatttgt actgacatcg tatttacgat atttccattt
     1141 ccttggactg cccaaccacc agcaatcaga attgcttgag gcttttgtgc caaataagga
     1201 atatggacga cccagtaagt ccaagcggca tagtatgcca gtcgtttatt agatgtcgta
     1261 ttttcgaccc atgaactgac gcctccttta ctatccttga aggctgatcc taattgtcca
     1321 acgatcaatg cgtatggaat aaaataaatg gccaaaatca atagccaaga ggtgacaact
     1381 gatactcctt gctgagcata gttattaact acattcccca tgccccaaac catgttaaat
     1441 gcaattaacg caacagtaaa ccagcgtaat tgtttgttgt tcattcgttc tctcctttat
     1501 gataaaaatt tgttatccag atgtaatcat attcaatttt taacaaactt acaagacagt
     1561 gttattttat gtgtgtattc ttttatttaa tacacatcgt tttttcatat tttttccatc
     1621 aaaaaaataa gttattttct tttatttcat tgaccttatt gaatttcaag ctattttgaa
     1681 ttttctgcaa aattaaaaac attagttatt ttataatcct atctattttc tttacatttt
     1741 tcattaagaa aaggatataa aaaaaagagg acgttcattc gtcctctttt tttacatgca
     1801 agcttcgcat ggcattcaaa acagctaata ctgtcacccc aacatctgca aatactgcat
     1861 ctcccattga tgcaaaacca aaagcaccta aggcaagtac caaaattttt atcccaatag
     1921 caaagacaat gttttgtttg acgattttca atgttttctt tgctaaatgg attgcttctg
     1981 caatcttcga tggttgatca ttcatgatga ctacatctgc tgcttcaatt gcagcatcgc
     2041 ttcccaatcc acccattgcg attcctacat ctgctcttgc tagcaccggt gcatcgttga
     2101 ttccatctcc gacaaatgcg gtctttttat tgtttctttg taagatttcc tctaaccgct
     2161 gcactttatc ttcaggaagc aattcgctat atatcttgtc cacgcctact tgcttcccga
     2221 tatgatcagc aatttttttc gagtcgcccg tcaacatgac tgtgttcttc acaccagcct
     2281 gttttaattg aaccaatgcg tctacggcat cttctttcaa ggtatctgca ataacaagat
     2341 aaccactgta actttgatcg atcgctagat aaagcaatgt accgatttct tgacttaaag
     2401 gaaaagagat ttcaaatctt tccatcaatt tgtgattacc tgctaatact tgtttgccat
     2461 caacttcagc taagatccct tcacctgcaa attcctggat attagttgtt ggtaaaagcg
     2521 gacctgcgta gccttctaaa acagattgag caatcggatg tgtagaaaac tgttctgcac
     2581 tcgcaatata ttgcatgaat ttctttggat cgatttttgt atcgatcgtc tggatcgaaa
     2641 agtcaccttt cgttaatgtg ccggttttat cgaataccac tgtctcggtt tgcgcaagca
     2701 actccaagta atttgaacct tttactaata caccgatttt acttgcgcct ccgataccgc
     2761 caaagaaact aagtggcacg gaaatcacta atgcacatgg acatgagatc actaaaaatg
     2821 tcaatgctcg atagatccat tcagaaaacg ccgctccaga aacaacaagt ggaggtaaaa
     2881 cagctaataa aacggccaat cctacaacga ctggtgtgta atagcgcgcg aatttagtaa
     2941 tgaaattttc agctggtgct tttttactgc tggcattttc tactaattct aataatttgc
     3001 tgatagtaga attttcataa gatttttcta cttgaactaa aaatggttta tttttattga
     3061 taaatccgct taatactgtt tcaccaacat atacagattt agggactgat tctcctgtaa
     3121 gtgctgacgt atcgactaat gattctcctt cgatgatcgt tccatctaac ggtacacgtt
     3181 caccaggctt cacaagtaca aactgaccga tctgcacgtc ttcagggttc acggtcatca
     3241 atccttccgt cgtctgcacg tgagctgttt ctggacggat cgccatcaat tcgcgaatcg
     3301 attttcttga atgactgact gccaatcctt ggaaatactc gccgatttga taaaatagca
     3361 tcactgctac ggcttctgga tattcaccga tcaacatagc gccaatcgta gcaacagaca
     3421 taagaaaatt ctcatcaaag atttttcctt tgacgatatt catcactgcc ttcttcacta
     3481 catcatatcc tatcagcaga taaatcaata aataccctat caagcgaatt ggttcattaa
     3541 aatcaaataa tagcaataaa gcgatacctg caagggaaaa taaaatccgg aacagcgtat
     3601 ttctctcttt ttcatttcca tgttcagata ctttttcctt atctatttct gttactttga
     3661 catctggttc taattccttc actacttttt ttgtttcttg ttcaatcgta gccaaatgat
     3721 cttcctctgc gtcaatcgtt agttttccag aagtgaagtt taccgttgct tccttgactc
     3781 cattgatctt ctgaactcct ttttcgattt tcattgcaca gttcgcacaa tctaaacctt
     3841 ctaatctgta acttttcatt ttaatgacct cctcgcctca acatatgaac atctcttcat
     3901 aactaattat aaatgaatgg ttattcatat gtcaaggtat ccgaaagaaa aaatgaaaaa
     3961 catctgcatc ggaaaatt
//
LOCUS       DBNICI010000194         3956 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.198, whole genome shotgun sequence.
ACCESSION   DBNICI010000194 DBNICI010000000
VERSION     DBNICI010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.198"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..156
                     /locus_tag="AC1ZCC_002613"
     CDS             <1..156
                     /locus_tag="AC1ZCC_002613"
                     /inference="COORDINATES: protein motif:HMM:NF012267.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIQ1630825.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="AC1ZCC_002614"
     CDS             175..1452
                     /locus_tag="AC1ZCC_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1630826.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="AC1ZCC_002615"
     CDS             1462..2118
                     /locus_tag="AC1ZCC_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1630827.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2118..3494
                     /locus_tag="AC1ZCC_002616"
     CDS             2118..3494
                     /locus_tag="AC1ZCC_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1630828.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3624..>3956
                     /locus_tag="AC1ZCC_002617"
     CDS             3624..>3956
                     /locus_tag="AC1ZCC_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_137665535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HIQ1630829.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1594 a          425 c          694 g         1243 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagaaag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa acaattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt tgggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
     2161 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
     2221 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
     2281 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
     2341 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
     2401 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     2461 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
     2521 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     2581 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     2641 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     2701 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
     2761 gagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
     2821 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
     2881 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
     2941 gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa tctatatcag
     3001 gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca ggagttgtag
     3061 tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca gtattactat
     3121 cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg gtatttatat
     3181 caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac aagcttggga
     3241 ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa caatctgcat
     3301 ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta tcaagtttag
     3361 atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt ttagtacttc
     3421 ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa gaattattaa
     3481 ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aatgagagtg taatataaac
     3541 cgtgtaagta accatcaatc ctaggcgggt ggttgcttca cggtttttat ttttctaaaa
     3601 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gctgagaagt
     3661 tagctgaatc cattttgaat acttatcaac ccgaatctgt agaggatatg caagatgttt
     3721 taaaagatgt gtttggtccc ttatttgaaa aaatgcttca aggagaattg aataaccatt
     3781 tagggtatga cgcccattct aaagagccaa agaaacatga caatcgacga aacggttatg
     3841 gcaataagac gcttaaaacg agctttggta aagtaaacat tgatgttccc agagaccggg
     3901 aggcttcttt tgaacctgag ttgattccta atagaaaacg agatgtctcc gacatc
//
LOCUS       DBNICI010000195         3946 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.199, whole genome shotgun sequence.
ACCESSION   DBNICI010000195 DBNICI010000000
VERSION     DBNICI010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.199"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(561..1601)
                     /locus_tag="AC1ZCC_002618"
     CDS             complement(561..1601)
                     /locus_tag="AC1ZCC_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HIQ1630830.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1950..2276)
                     /locus_tag="AC1ZCC_002619"
     CDS             complement(1950..2276)
                     /locus_tag="AC1ZCC_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630831.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2263..3051)
                     /locus_tag="AC1ZCC_002620"
     CDS             complement(2263..3051)
                     /locus_tag="AC1ZCC_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIQ1630832.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3419..3709)
                     /locus_tag="AC1ZCC_002621"
     CDS             complement(3419..3709)
                     /locus_tag="AC1ZCC_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HIQ1630833.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
BASE COUNT         1223 a          655 c          554 g         1514 t
ORIGIN      
        1 tccgttgcgg gtaataccgt aacactgaag ggcacgcatt aaggcgtcat cttaatgctt
       61 tcctgtagga cactgacgga gattaccagt ctcccgagcc ttacaggggc ctttcttttt
      121 tttgtatttt caattatcac aatttatcgt ccaaaagtgc agaacgaaaa tcaattcatc
      181 acaagcatat cagacaatta aatttaaaac aactaaaaat atacaaaaaa gtgtaattga
      241 atattagcag cactgcctga aaagaagtaa tatcaggctt ttcagcttgg ggaaataagt
      301 gaattacctc tcttcatgaa ttattataac tcaaaaaagc acatatgcaa acactagttc
      361 cctatttttt tgaatgaaaa ataactaaaa tatctaacga ttatgacttt aaaacctacg
      421 tttgtgattg ttttcataaa cttttaatct aaaaactccg gaataatagc tatttatttg
      481 taacatttgt aataaaatta aaatcataaa aaagttctaa tttaaaattg atcattttta
      541 tagtgatcgc tttttatcaa ctactctagc caattttccg tactaactgt tggtaattcg
      601 ttaaatgttt ctctttgttc gtttgcaagc tttgccgcaa tagctttttc gacaaaccaa
      661 ttttttacat aaacgaaaat tagattctgc aaattgttta ctttttcagt tttttgtttt
      721 tgataagctt tcagcaaagt catatataat cctttgtcag catcaattcc ataagattcc
      781 agttcttcat aaacaacaac tgtttgctct aattcttcag cttttttctt tgcattatga
      841 atagtttgga cggttttttg agcgtcttcg taagtactag aaaatgtttt tatcaagttt
      901 aatacatttt ctggtacaaa tgtatttata ctgtcctctt tcatgatgtc gacgaagtta
      961 tctagtaaaa cttgattttg taactgatca gtttcagtgt ctattatgtg tctattagtg
     1021 tctatagaat tagaatagaa tagattgtct gcgattttcg caattacggt tgagtccttg
     1081 atatgactgg attttgccgt atttgcgatt ttcgcaatta cggtggtatc attgatttct
     1141 cgcggttttg ccgtatttgc gattttcgca attacggtgg tatcagtatt gtcttcctct
     1201 tccatgattt taaagacgtc ttgattcgtt acttctggct tgcctaagta caataaatta
     1261 ggcatggatt caatcttacc gtcacgttta tttactcttc ctctcttctg tttcagaaga
     1321 cctgcttttt ctaattcctt tttgatttta gatactttac cttcgcggca ttgtaacaat
     1381 ttcattaatt cttcaactgt gaatatgaaa aatatattat catcagaatc tacccagtta
     1441 tttttgactg aataatcaaa tcgatcttta agtagcatat aagcaatttt tgcatcattt
     1501 gatagatcca tgtatttttc gttagtaaaa aataccttag gcatttgata atatttttca
     1561 cgataaattt cttttgattt gtaataatta aaatcattca tagccaatcc tctttctgta
     1621 ggatagcgtt ttgttcaaaa ctatgttaca atgaattaaa caaatatatc tatccactat
     1681 atatttttct actgctgata agtcgccaaa ctttgttaaa gcagtagtct ttttttatct
     1741 ttttttagtt taccttaaaa aataaattaa agcaaaccgc tctatttcaa ggtttaagct
     1801 catagtaact atatcacgat attacaataa tattatatca cgatatcgta atattacaat
     1861 atgaagataa gcgacttatc agctttgata ctactgattt ttataaaaaa aatatcacga
     1921 tattatgata tcgtgatatt gaatgtaaat taaattttat tttgtttctt tgctagttta
     1981 tatccttcaa tcaagtattc ttcaggtatg gactttaata gcgcatcttc tataacgtct
     2041 ggtttacttt tgtctgattc agcaatagtc gtccaatatt tgagtgctga cattagaatt
     2101 ggatttatac gaatgctatc tgctgttctt acactacttc cttgatcttt tatgacttta
     2161 cttatatttt tcttggttaa ttcgatttct tctacttctt ttgatttaaa attcttttta
     2221 tttttatttc ctagtaatct acttactgca gcttgactgc gattatcttc tgccatcaaa
     2281 atcaccaact tcttttaaat attcttctgt aaatgcttta tagtcatctt cagctgttga
     2341 gggtttatta aaattgatta gatctgatcc agcagcttgc gcttcgtcaa cggttattgt
     2401 cctacgaata taagttttga aaatttttgc accaatgact ctagaaattt cggttgctgc
     2461 agacgtcatt tctttagaaa tgttataacg cttattatac ttagtaaata gaactcctcg
     2521 tacttcaagc ttgtcgttcg tatattcttt taccgtttca atagtttcac ttaactttat
     2581 tagtcctttt aatgagaaat acgcagcttc aactggaatg ataatttcat cagaaacagt
     2641 aaatgcgttg actgtaagca ttcctaaact tggtggacaa tcaattataa tataatcata
     2701 gttgtccttg attggttgaa gtactctttt aagcctgtgt tcacttccta cttgagtaaa
     2761 ttccctctct gcagaagaaa gggctatgtc ggctggcaaa acatcaattc ctttatagtc
     2821 cagaatagca tctgtaatgt cagcctcccc ttttaatact tcataaattg taggttggtt
     2881 tgttccatct atatcgaaac ctacattatc tgtagcattt ccctgaggat caatgtccac
     2941 taaaagtaca tttttaccgt aaacaaattt caaacatgct gctaaattaa cgctagtcgt
     3001 agttttagaa actccacctt tttgattggc tactgacaat attcttgcca ttaaaatatc
     3061 ccccattcat cttatattat aatataatta tatcataata ttgtgatatt gcaatatcgt
     3121 atatgcatta tattgtaata ttgtgtgatt attttgtttt aaagatatat atcggttcgg
     3181 atataaagat tattcatatg ttattcgaaa ttaaaataat cactttttat tactttaaaa
     3241 ttttttatta atgataagaa atatgttttg cgagcagcaa ctagaataat ttaaacatta
     3301 ctaaactgtc tatattgtta ctaataattc ccttcttata gtgcattttc ttagaaaaaa
     3361 acaatattac gatatcatga tattaagata tcgtaatatt atacaattgt gattgatatt
     3421 atatatgttt ttttattttt ttatcatgtt taatcttgaa ggaatcatat tctttttcta
     3481 aagacaggag cttttcataa acatcagaca tgtttaaact agaggccagg cttaaatcat
     3541 aaacgaatga agcaggtaat gattttattc tacgttgtcg ggttatccag cttgcaaccg
     3601 ttccttgtgg gtaggcatat aactcacaaa attgttctac agtgattcct aaattcttga
     3661 taatgtaaac attgatcggg tgcgagtaaa caaatgtatt gatagacatg gtgcgtaagc
     3721 ctactttctt ttattattgc gtttgcaata atagtttaac acacgcatta ttctagtatc
     3781 tactcctatt tgtaaaaaca aaaaaggatt catagagata actctataga atccgtaaaa
     3841 taccttatat atataacggg aagatataaa acctaaaatt atgataacct gctgcagctt
     3901 ctacatagaa agagaagcgc tcaacaccaa cctcatttat caaacg
//
LOCUS       DBNICI010000196         3877 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.200, whole genome shotgun sequence.
ACCESSION   DBNICI010000196 DBNICI010000000
VERSION     DBNICI010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.200"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(135..1550)
                     /locus_tag="AC1ZCC_002622"
     CDS             complement(135..1550)
                     /locus_tag="AC1ZCC_002622"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HIQ1630834.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(1567..2883)
                     /locus_tag="AC1ZCC_002623"
     CDS             complement(1567..2883)
                     /locus_tag="AC1ZCC_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIQ1630835.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(3033..3731)
                     /locus_tag="AC1ZCC_002624"
     CDS             complement(3033..3731)
                     /locus_tag="AC1ZCC_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIQ1630836.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT         1173 a          858 c          607 g         1239 t
ORIGIN      
        1 cagctttttc acaacctctc aataaacggt gttcaatcag ctgaccctgg gtattagctc
       61 aaaacgctgg attcacaacc tcttttattt caaataacca gtaaatacaa tgaacatcta
      121 caaaaaactt ctatttattt atagatttcg tcaggcaaat ccaattctct cttatcaatc
      181 aaagtctgat accacgaagc agattttttc aatgttctgc ttcgttcatc gttcaattcg
      241 attcgaacta atccatatcg attcttaaat gcattcattg gtgacacaca gtcagtgaat
      301 gcccataaca tatagccttc gcaatttgcg ccttcttctg ttgctttgat caagtaatgc
      361 aaatgctgtc cgataaattc gatacggtaa tcatcttgca cgattccttt ctcatccata
      421 tactgttctt catattctct tcccataccg ttttctgtta tcagccaagg gatattatga
      481 taattttctt ttagatacat ggctatatca tacatgattt tcggatagat ttcccatccc
      541 ctggaattat tcattttctt cccaggtaaa tcaaagactt cataatatcg ttcaggatgg
      601 aaaggtgtct cttcattcca ttgataacga ggggcgcgaa cgcgtttagg ataatattga
      661 ttgattccaa ggaaatctac tgtatgatgc tgaatcgttt ctaaatcctc tgttgcagga
      721 tcgaaataaa tcgattgttt ttgacacaac tccaggaact cttctgtgta gtatcctttt
      781 accatcggat caaaaaatac tcgattataa aacaaatcat acatataagc agctttttgg
      841 tcttctggag aattggatcg agcatagacc atttcaggat ttaaaataca gccaatcctt
      901 ccaccataat ttccgctgcg gtaaatctct actgctttag ccgttgctaa aactttatga
      961 taattccaca tcatccactt ttttgtgttt tgttcatgcg gccagcgaac agcatccaaa
     1021 tagcaacgcg tttggggaac gattggttcg ttgaatgtaa accagttttt taccctatca
     1081 ccgtaacgat caaaggcaat ttgagcatac ttagcataaa aatcaactac ttttcttgaa
     1141 gaccagccat catatttttc caaaagaaaa gctgggactt cataatgttc caaacaaatc
     1201 atcggttcta cgtctgcttc gatcaaagag tcgatcattt cacttatatg ctgagcatat
     1261 tcttcatcta ctatacagtt ctcataatca gtaaaaaaac gagcccaatt gatagaagtc
     1321 cgataatgtg tcagatggat ggatttcatc agatccacat cctgctggta ttgttccata
     1381 aaattagttg ctacggctgg gccataccca ttatgccaaa caaaatgttc atttttatac
     1441 cagctatcta taaacgaatc ctgtccttct ttttttcctt tccaaccttc cgtttgccaa
     1501 gcagaagatg cagcaccaag aataaaattt tttggaatag taattttcat gatttcctcc
     1561 tgttttttag atcccatcat tttccacacg atttgctact tttacaaatg gcagatagat
     1621 caaaatagaa acaataatac aaacaagttg cgtaataact gcaccgatat ttcctgctgt
     1681 tgccagataa gcattgacga tcggcggcat tggccaaggg accattacaa ctgctttcgc
     1741 cgcaaatcct aaagcggttg caaaataacc aatcgttcca gtgataagag gcgttaagat
     1801 aaacggaata gctaaaattg gattcagcat gattggcata ccaaaaataa ctggttcatt
     1861 gatgttgaag atcccaggag caagggataa cttggtgatt tgtttcatat cttcccgttt
     1921 tccaactaac aatactgcaa tcagtaaacc gatcgtgact ccagacccgc ccatactcat
     1981 atacatatcc caaaatggca tagtaataat gttcggtact tcttttccag cttcaaaagc
     2041 ttccgtattt acagcaattg ccgctaaaag caacggttcc cgtatcggct tcaccatttg
     2101 atttccatgg ataccaatca cccaaaagac ttgcgcaata aacatcaata gcaaaatacc
     2161 cggtaaccct tgtacgacac cttctaatgg gcgttgtacg acattgtaaa tgatatcata
     2221 aaggtacatt cctgtaagtt ctttgatgat aaatcccgca gccgcgatac ccgatacagt
     2281 cagaatcgtc gggaataaag cagaaaaact agcagaaaca ttcgttggca cggtatcagg
     2341 cattctcagc tgtagtttct tctgtttcct caaccagcaa taaagttcaa tagaaaggat
     2401 ggagaccaac attcccaaaa ataacccttt cgtatctgta tattgtttag ccaatacgtt
     2461 ttctactata acagtctgat tttcattcac taattcataa acagttggat taacagataa
     2521 ataagacatc actgcgatga taccagggaa tacaccatcc actttattca gcttggctaa
     2581 ttcgatccct atcagaaaaa cagcatagat ggtcaagaaa cttagtgtga catagctgat
     2641 ggcagatgcg ataggcttta attcttctaa aaaacgcaag aaacttatct gtgctaagcc
     2701 attttccgag tcaaagacca ttgctgaaaa caatgtgcca aaagcacccg taataattac
     2761 cggaatcaat gcagcaaatg cgttttttat caccatgata taccttaaat tattgattct
     2821 cgttgccacg ttccctaaaa cattggctaa tttatccata aaactcattc cagatcccct
     2881 cattcctgct tcttctcctt gacgtcatct ctttttgtct attagtcagc gcttacattt
     2941 cgatgaacag aaactattta ctcctctttt ttaccaaata taaaacaaaa attcatctga
     3001 tgtatttatc ataaaataca tcagatgaac tgtcaattag tttctttttc tcctattatt
     3061 ttctgtaatt ctctccgatt tattgccata tggacagtca ttgcatcaaa cgcagcaagt
     3121 gcattccgcc tttcgatggc ctgaacgatc tctgtatgaa tatcgatcgt ctcgattttc
     3181 agatgctgga tatcataatt tttattaaac aaagcaattg attcattaat gattgggata
     3241 atatgcagca aggctacgtt tccgctagct tctgcaatca tcgtatgaaa atcgatgtct
     3301 aattgaacat gttcttgtgt gccttctcga aaagaagttt caatcttttg cgttaatttt
     3361 ttcatttgga cgatttgatt cttcgttgca tattccgctg ccaaacctgc catcttaggc
     3421 tccaataaat atctcagttc aaataagtct cgtatcatcc gatcctgatc ttcaatcaaa
     3481 gaaaaaccga gtgggtcatt ggcaatccct cgcttatcac tgacaaatgt ccccgctcct
     3541 tgccgaactt caaggatatt cctggaagct agagcccgca cagcttctcg caccgtgctt
     3601 cgccccacct ctaaataatc ggacaattca tattcattcg gcagtttatc tcctacttgc
     3661 aactgcattt cctttaaata tctaaggatc ttgtcagtag ttacttctac tagggtttta
     3721 ttttcttcca aaccacatac ctccttttca tctgatgtat tttttcgtaa ttattataac
     3781 acgtttgaat catctgatga ttttttgtat acaaaattga ttaatccgtt catagacata
     3841 caaacgaaga agatatttac cataaaaaag actagga
//
LOCUS       DBNICI010000197         3817 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.201, whole genome shotgun sequence.
ACCESSION   DBNICI010000197 DBNICI010000000
VERSION     DBNICI010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.201"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            352..1041
                     /locus_tag="AC1ZCC_002625"
     CDS             352..1041
                     /locus_tag="AC1ZCC_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIQ1630837.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="AC1ZCC_002626"
     CDS             1028..2236
                     /locus_tag="AC1ZCC_002626"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIQ1630838.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     misc_binding    2275..2480
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="AC1ZCC_002627"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="AC1ZCC_002627"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIQ1630839.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
BASE COUNT         1258 a          658 c          771 g         1130 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaag
//
LOCUS       DBNICI010000198         3762 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.202, whole genome shotgun sequence.
ACCESSION   DBNICI010000198 DBNICI010000000
VERSION     DBNICI010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.202"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..218)
                     /locus_tag="AC1ZCC_002628"
     CDS             complement(<1..218)
                     /locus_tag="AC1ZCC_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HIQ1630840.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYYQKLTDNPIGFGLLPEDDEGNE"
     gene            complement(837..1169)
                     /gene="traE"
                     /locus_tag="AC1ZCC_002629"
     CDS             complement(837..1169)
                     /gene="traE"
                     /locus_tag="AC1ZCC_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HIQ1630841.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYAGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            complement(2016..3056)
                     /locus_tag="AC1ZCC_002630"
     CDS             complement(2016..3056)
                     /locus_tag="AC1ZCC_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HIQ1630842.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFIPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SFPKTITKDWLE"
BASE COUNT         1231 a          662 c          496 g         1373 t
ORIGIN      
        1 cattcattac cttcatcatc ttcaggtagc aagccgaatc caattggatt atcagtcaat
       61 ttttgatagt ataaatcatc ctcgtctaat gctaaaacat ctaatccttt acaatttccg
      121 cctcttctaa acgtcacaaa tcctttgtaa tcccaacgtt ggaaagaaac cttaatagta
      181 tgtgtgccca atcgtatatt cggatcataa tttttcattt gtcattcctc cattacgtaa
      241 atttcatttt ctctatgttt tatatcaatt ttttcacttt attcaggact cgttcatatt
      301 ttatcgtttt tcgtttactt aaaaatacat aaaaaatatc gactcgttta tggcgtccga
      361 aacgagaaac cctagtagaa atcggtaaat gctcgattga tccaatgacc aacgtcctcg
      421 caagtatcta catgtactgc agcacacgac acaatttctt gtgcgtcctc cccattacga
      481 gaagacctac tccttattcc atgagtttgc gtcaaagcct agatccctta gtagctgctc
      541 atgattaatc taggcgctat tctaacttgt ctaacttcta aattccctct tgtttactct
      601 gtgcatatta tttacacaaa ttcatttcac cactatccaa acaagggcat ttactcgatt
      661 gaccaatttt ttacgttacc aatcgtaaaa acctaccccg tcaccccccg aaaggaattg
      721 ttgatagatc aagtctactc cttatttttc tagtaattct aagttctttt agaagtctcg
      781 acaatatttt caagcaagcg aaaaacgata aaatacaagt tatgagaatg atcaattcag
      841 ctgattaaac gccccatccc ttttgcggta caacgcaaga ctccattttt tcttcgacca
      901 atcagtacac cgttttggtc ataaacatgt ttgattgttg gatatagttc ttttaaaggg
      961 ataggatctt ttaaaattat ttgcttttgt ttaccagcgt aataaatcat ttcctctgag
     1021 actttgtctt tctggattat atatttctta cactgcacaa cggttttact ttggccagtt
     1081 gatagtttct tcgtgatttt ctctgtagcg gtacaccgtt taccgtagtg acaaatatct
     1141 tcgagactca aaattcctgt atatgacata aaataatcca cccacttact ttaaatattt
     1201 ttaataagtt tttcttgtta tttttcaaaa atagagaatt gattttcgtt catgacaaac
     1261 aaaaaagagg gaacgcatcc aaacatgcgt tccctctaat ttcagcgttc gtcatacggc
     1321 aaagagccac acggacattt ttataacatt atgcttaggc atagattcat cgtaatatca
     1381 cgtattttta accttaacac agccaaaaaa agtggtatca cttttgaata tctgttatac
     1441 taaatccgtt gcgggtaata ccgtaacact gaaaggtaaa cgcattaagg gattaaaggt
     1501 tgtcttaatg ccacctgtgg acgtcgacga aggttaccag ccttccgagt ctcacagggc
     1561 ctttcttttt tttgtatttt caattgtcca aatttatcgt ccaaaagtgc agaacgaaaa
     1621 ttaattcatc acaagcgtag cagacaatta aatttaaaac aactaaattt atacaaaaaa
     1681 atgtaagcga ataatattcg tattgcctga aaagaagtaa tatcaaggtt tttagctttt
     1741 ttaaaaaatg aaattacctc tcttcgcgta ttattataac taaaaaacag caacatgcaa
     1801 acgtttgttc tcttttttgt gttggaaatt aattattaac tattaacact tatgacgtaa
     1861 aaagctacgt ttgtgatttt tatcataata ttttaactca taaattctat taaaatagct
     1921 atttctttgt aacatttgta atataattag aataataaaa aatttttatt tttaatgcta
     1981 ctccatgaag caacaatcaa tttgtcagtg aaatttcact ctaaccagtc tttcgtaata
     2041 gtttttggga agctttcaga attcaaacgt ttttcacttg atactttagc agctattggt
     2101 ttttcgataa accaattttt tacatagaca aaaatcaggt tctggatatt ttcaactttt
     2161 tcggttttta atttttgata ggcttttaac agtgtcgtgt ataattcttg ttctgcattg
     2221 acataataac tatcgagttc ttcaaaaatt attgaaatac cactttctgt ttcggctttt
     2281 ttctttgcat tatgaatagt ctggacagtt ttttgagctt cactgtaact tgaagaaaat
     2341 gtttttatta aatttaatac tcgttcaggg ataaacgtag caatgctgtc gtttgtcata
     2401 attgtctcaa aattgtctaa caacaattga tcttgtaggc ggtctttttc agtgtctata
     2461 ttgtgtctat tagtgtctaa tatattatta ttaagttctg gtatgatttt cataccctct
     2521 gatttatcaa cgttttggca cggtatgatt ttcataccct cttgagcccc aagggattca
     2581 gcgtctgatt tttcgtcgag ggtgggtatg attttcatac cctctgattt atcaacgttt
     2641 tccctattct ctctatcaaa tttttcgagt aagtagatat cattttctga gacttttaat
     2701 tctgctagat atagtctatt aggattattt ttccctaact gtttgttaaa tcccatctgc
     2761 ttttgtaata ataaaccaaa ggattccaac tcttttttga ttgctatcgc tttttgtttt
     2821 tggcagttta atacgctaca tagttcattt gtcgtataag taaaataaac attaccttct
     2881 tcatcgattt gccgtttgct gattgcgatc tctaatcttg cttttaaaag catataggca
     2941 atcttagttg aatctttcat ttttttgtat tcgtcagaaa caataaagac ttttggaaat
     3001 tgataaaata attcgttgta aactctatcc gccttaaaaa aattaaaatc agtcattgta
     3061 gatcctctac tttcacgttg ttttatttat gtaaaattac ctactctttt cgaaaatagc
     3121 aaagataaat aaaaaatgca aacgcattaa aactgttttt taattaaata ctacctaaaa
     3181 tcagaaataa aatgaattat gtactacctt taaagtcatt aagaactact ttaaataaat
     3241 attatttata attaaatact accagaagaa cgtatgaata aaatttagtt ttaaaattaa
     3301 agataattaa gtactaccag tgtcaaaaca aaaaataaat taagaactac cttatgcata
     3361 taaaacatac taccattact agtaatatat actaccttat ttcagtagta tatattatat
     3421 tcttcttata agacaaggct ttaggctaga aaacaataga ttatatacta ctaaaaaata
     3481 caataagtac taccaatgtc agatatatat aataattatg tactaccatt aaaattaact
     3541 tatttaataa gatactactg tgttaatctg aaaaaaatta ctttaaagta gttacatact
     3601 acctgttaaa ttagatacta ccattaatac ttaaattaga tactacctat atttatatta
     3661 agtactaccg ttctaggaat aagataataa aaaaaaacta ccaaattgca attaaatact
     3721 acttaaaatg taaaaaataa gaactaccca aattaggtag tt
//
LOCUS       DBNICI010000199         3701 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.203, whole genome shotgun sequence.
ACCESSION   DBNICI010000199 DBNICI010000000
VERSION     DBNICI010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.203"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            82..1431
                     /locus_tag="AC1ZCC_002631"
     CDS             82..1431
                     /locus_tag="AC1ZCC_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIQ1630843.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1559..2149)
                     /locus_tag="AC1ZCC_002632"
     CDS             complement(1559..2149)
                     /locus_tag="AC1ZCC_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HIQ1630844.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2296..3648
                     /gene="brnQ"
                     /locus_tag="AC1ZCC_002633"
     CDS             2296..3648
                     /gene="brnQ"
                     /locus_tag="AC1ZCC_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HIQ1630845.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1041 a          688 c          731 g         1241 t
ORIGIN      
        1 ttttggaatc ttagttgtgt ttttcagaaa actctcttaa tatggaagta attaaacttt
       61 tttagttagg atgatttaaa aatgaatgaa gagcaagaac tgcagcgtgg actgaaaaat
      121 agacatgtcc aactgatttc tattggcgga gcgattggaa cggggttgtt tttaggttcc
      181 ggaaagtcta tccagctagc tggcccttcg atattgctcg cctatcttat tactggatgc
      241 atctgttttt tcattatgcg tgcgttaggc gaacttctgt tatcaaacac caataaccat
      301 tcttttctgg attttgttgc agaatatctt ggcaaaaaag cggctttcat cactggctgg
      361 acgtattggt tctgctgggt atcgattgca atggccgatt taacagccat cggaatgtat
      421 gtacgctact gggcgcctgg tgttcctcag tggcttcctg aactgattgc attaggtctt
      481 cttctttgct tgaatatggt ggctgtctca ttgtttggtg agttagagtt ttggtttgca
      541 ttgatcaaag ttgtagcgat cattgccttt atcctcgtag gagcttatat gatcctcacc
      601 cactatacga cagatattgg cacagccagt atcacgaatc tatggtctca tggcggattc
      661 tttccaactg gtgcaaaagg atttatctta gctttccaaa tggtaacctt tgcttttgct
      721 gggatagaat tagttggatt agtcgctgga gaaacagaaa atcctgaaaa agtgttgccg
      781 gaagctatca ataatattcc tatcaggatc atcttatttt acttaggatc tctttttgtc
      841 attatggcaa tctatccatg gaacagcttg aatccagata acagtccatt tgtagaagta
      901 ttttcggaaa ttgggatcac gattgctgct tccttgatca atttagtcgt attaagtgca
      961 gcagcttctg cctgcaacag tgcaatatat agtacaggac gtatgcttcg ttctttagcg
     1021 caagaaggca gtgcccctaa aaaattcaaa aagttaacaa cacatcatgt gccagggaat
     1081 gctttgacat tttctactat cgtcattttc atttctgtta tcttgaatta cgtgatgcct
     1141 agtgaagtat tcacactagt aagcagtatt gccactactt gtttcttgtt tatctggagt
     1201 atgcttgtct acacgcacat gaaatatcgt aaaagtatcc tcggcaaaaa agctcattca
     1261 tttaaaatgc cgttgtaccc atttagtaat tatctggtat ttctttacat ggcctttgtg
     1321 tgtgtcgtct tgttcctagg aaaagatacg agaatcgctt tattactaac acctgtgtgg
     1381 ttcttgcttc tattactaat ctatcatatg aaatatgaac gtaagaagta agaaagtagt
     1441 tgtgacagaa gtggtcagct tcgagaaata agaaggaatt tacgaaaatt gcttcttaaa
     1501 tttttgtgaa atttcggctt atttcttgga gataaaagct tctgtcccag cctcttttct
     1561 atttccccgg aaacaaccgc tgataatagg catctgcttt tttataggaa aaaatccaaa
     1621 taccagatac aaaaaaacgt ccgaataacc cgatcaacgg tacaaaaaag acaccggcaa
     1681 tcaagcataa gttaatcatc atggagataa gacttcgacg gtcctttgac aaaactctca
     1741 cgacatggga atctggttta tttgctttca tgatttgcct catcaagata ccaaaagaaa
     1801 aactccacat cgtgtatact aagacataag ccatctcagg ccaaaaagct tcagggttac
     1861 gcccgaccca actagtaaca gaaggacata aagtcagcca aaagagcaaa aagatattcg
     1921 accagaccac tcggtaatta attcgttctg ccatctgaaa tatacgatga tggttcaccc
     1981 agatcaccgc aacaaatata aagctgatgg tataagctaa taatgtattt cggatgaccc
     2041 ataaagaatg aaagctagat gtattaggga tcttgatctc taatacaaga actctcaaaa
     2101 caatggctac cactccatca gtaaatgctt ctaatctcga ctttgtcacg atgattcctc
     2161 ctcagatgat tcagcgcttt tattttatca cttacgttat taaataacca tcgttttata
     2221 tagggaattt tctgaaaatt gttgcatatt tttcgaaatt ttgttacttt taaaaaaacc
     2281 gtaaaggagt tatgtatgac aaaaaaatta tcatttaaag actatctttt tataggctcc
     2341 atgcttttcg gtctattttt tggtgctgga aatcttatct ttcccgttca tttgggtcaa
     2401 gaagcaggcg ctgctacctt ttgggcgaat ctaggtttct tggttaccgg tatcggtctt
     2461 cccttcttag gggtgattgc cattggtgta tcgaaaagct caggtgtata tgaattagca
     2521 aaacggataa atcaaacata tgcgatggtt tttaccatct tgctttactt agttatcgga
     2581 ccgttttttg ctttacctcg ccttgcaacc acatctttcg agattggttt agcaccattt
     2641 atcgatccta gcagacagac gctatatcta gcaattttca gtattgtatt ttttgtgctg
     2701 gcttggtggt tttcaagaaa accgacaaag atattagatt atgttggaaa gttcctgaat
     2761 cctgcatttt tggttttatt aggtgctttg cttctccttg cttttttgaa tccactaggt
     2821 tctgtaaatc aagcgcctat tcagccaaat tatcaagagc atgctttttt cacagggttt
     2881 actcaaggat ataacacatt agatgcgctt gctgctttag catttgggat catcattgtc
     2941 acaacaatcc aaaacatggg tgtaacaaaa cctgctgaaa tcgctaaaga cacgatcaaa
     3001 tctggtgcga tcagcattat attgatggga atcatttata ctttgctcgc ttatttaggt
     3061 gctatgagct taggtagctt tgctctaagt gaaaatggcg ggatcacgct ggctcagatt
     3121 gctgatcatt atttaggtac ttatggcagt atcttattag catttatcgt tatattagct
     3181 tgcttgaaga ccggtattgg tttgattaca gcttttgcag aaacattcac agatttgttt
     3241 ccgcaaagaa attacgcatt ctttgttgca cttgctagta tccttccttg tttagcggca
     3301 aacgtaggct tgacgaatat cattcaattt tcgttgcctg ttttgatgtt catctatcca
     3361 ttagcaatga cactgatttt actagtgtta gttggtcctt tgtttaagca gcgtacggct
     3421 gtttatcgaa tgactactta ttttacattg attgcttcga tttttgatgg gttgaacgct
     3481 tgcccagaaa ctatcaaaca aacacctatt gttcaaaatc ttttatatgt tgcggaaagc
     3541 tacttgccgt ttttcaaatt ggggatgggg tggatcgtac cagcagttat cggttttgtt
     3601 atcggactga tttggagttt cgctaagaaa gaagaagtag cagattgata aaagaggttg
     3661 tgaaagagac gttcagcctt gagcaataaa aagtaatttt g
//
LOCUS       DBNICI010000200         3693 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.204, whole genome shotgun sequence.
ACCESSION   DBNICI010000200 DBNICI010000000
VERSION     DBNICI010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.204"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            172..516
                     /locus_tag="AC1ZCC_002634"
     CDS             172..516
                     /locus_tag="AC1ZCC_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIQ1630846.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="AC1ZCC_002635"
     CDS             517..936
                     /locus_tag="AC1ZCC_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIQ1630847.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1028..1378)
                     /locus_tag="AC1ZCC_002636"
     CDS             complement(1028..1378)
                     /locus_tag="AC1ZCC_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630848.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="AC1ZCC_002637"
     CDS             complement(1344..2675)
                     /locus_tag="AC1ZCC_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HIQ1630849.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2931..3497
                     /locus_tag="AC1ZCC_002638"
     CDS             2931..3497
                     /locus_tag="AC1ZCC_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIQ1630850.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
BASE COUNT         1370 a          627 c          716 g          980 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggcaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaatttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat agtagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttctt
     2701 taactataac ttgaaatgaa agatttggac aactatcatt taggtcaaaa ctaaaaatgt
     2761 aagggaaccc attgaattta accaaatttt tttaattttg caaaaaaaag ttaaagaaga
     2821 ctgaaaaaaa atttaaaata taacgttttg agtaaaatgt ttgcaaaaaa tgctaaaaat
     2881 agggtacaat aaaacaacat atactgcagt aaagaagtgg gggagtttta atgagaagaa
     2941 aagtctatac caaggatcaa attctaaaag ctgcttatga tgttgttgca aaggaagggt
     3001 ttaaaggatt tactgcgaga aatattgcta aaaagatggg gatttcgaca cagccgattt
     3061 atctggaatt caaaaacatg gaagatttaa aaaatacgtt gctggattct atctttgaac
     3121 aactaaaaga agatgttttc cctattgaac gtactggtga taaaatcatt gatctcggtt
     3181 tgaactatat ctactttgca aaaaagcatc atagtcttta catggcattg tacattgatg
     3241 aacatgggga cggaaagaaa atgcatgatt tttcatacaa ttatttccgt gaaatagtta
     3301 aagaagatga aagatacaat caattatcgg atgaatatgt cgatgctctg catactggta
     3361 catgggtagc agtgactggc ttagcgtcat tgatgtcatc aaacatcatg catccaacag
     3421 aagaacaaat cattgcactg atcaaacgga caattgactc gattttagca ttagataatc
     3481 ctgaaataat cagatagtgt tctatcagta tgaatttgat cggtaagtcc gttggctgca
     3541 aactgtcaat ggactttttt gctaaaaaaa gcgatttctg ttatttatct attagaaata
     3601 ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta cggaaggttg gcagagtggt
     3661 aatgcaccgg actcgaaatc cggcgaaccg gct
//
LOCUS       DBNICI010000201         3584 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.205, whole genome shotgun sequence.
ACCESSION   DBNICI010000201 DBNICI010000000
VERSION     DBNICI010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.205"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(43..1962)
                     /gene="tet(M)"
                     /locus_tag="AC1ZCC_002639"
     CDS             complement(43..1962)
                     /gene="tet(M)"
                     /locus_tag="AC1ZCC_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HIQ1630851.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            complement(1978..2025)
                     /locus_tag="AC1ZCC_002640"
                     /pseudo
     CDS             complement(1978..2025)
                     /locus_tag="AC1ZCC_002640"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2339..2416)
                     /locus_tag="AC1ZCC_002641"
                     /pseudo
     CDS             complement(2339..2416)
                     /locus_tag="AC1ZCC_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(2570..3190)
                     /locus_tag="AC1ZCC_002642"
     CDS             complement(2570..3190)
                     /locus_tag="AC1ZCC_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1630852.1"
                     /translation="MKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIERGLNY
                     PAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGHYDNFF
                     DSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDDLYSMD
                     MFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
BASE COUNT         1067 a          744 c          559 g         1214 t
ORIGIN      
        1 tatttaacaa gaaaccatat ttatataaca acataaaata cactaagtta ttttattgaa
       61 catatatcgt actttatcta tccgactatt tggacgacgg ggctggcaaa caggttcacc
      121 ggtagtaaca tggtaccctt ttaactctgt taaacaaaca ctacgtccat ttgtaaagaa
      181 agttaaatca ctacgatatt cttgaataca ccgagcaggg atttctccac taagaatgac
      241 ctcattattt ttcaattgag tgtctacgat gttcgcacaa tatttaggag catcgttgta
      301 tgctcgtgaa agatattcct gtggcgcata aattttaaaa ctaagatatg gctctaacaa
      361 ttctgttcca gcttttttta agacttgttc caatacaata ggagcaagca tccgaaaatc
      421 tgctggggta ctaacagggc tatagtataa gccatactta aaacagattt tacagtccgt
      481 cacattccaa ccatacaatc cttgttcaca gccatagcgt atcccctcca taactgcatt
      541 ttgaaacgat tgatttaagt atccaagaga aaccgagctc tcatactgta ctccgctccc
      601 taatggaagc tgtgctacag atagaccaat ggaagcccag aaaggattcg gtggaacttc
      661 gatgtgaatg gtatactctg ctttttttaa cggtctttcc atataaatga ctgtaggctc
      721 ttttatttct atctccacat gatacttttc ttgcaacagt gcactaatca cttccatttg
      781 tactttccct aagaaagaaa gtataatttc atgtgtcgta gaatccacgt aatatcgtag
      841 aagcggatca ctatctgaga tttccaaaag ggcatcaagc aacatttctc tctgttcagg
      901 tttactcggt tcaacagttg tttgtagtag agggtgcgga ttttcaatct tttttctctg
      961 tggcaatagt tttgtatctc caagaacact atttaacttc aaaaactcat tttgcaaaat
     1021 aacaatttcc ccggaataag ccttatcgat tttacataat tcaccattta ttgaagtata
     1081 catttctgta atttttattt tttccttttc cgatattcta accgaatctc gcaaatgcag
     1141 tacgccacta taaagacgta tatatgcaag acgctgtctt ttttccgaat actcaatttt
     1201 gaaaactttt ccgcaaagtt cagactgacc tcgatgtgtt gatgaataaa atttattcgt
     1261 aatcacttct ataaggttat caatccctat attgtttttt gcacttccgt gataaacagg
     1321 gaacagggaa caattatgaa atcttatgct ttcctcttgt tcgagttcta atgcttccaa
     1381 taatttccca gacgtatatt tctctaaaag gtcatcgttt ccctctatta ccgtatccca
     1441 ttgttcagat tcggtaaagt tcgtcacaca catattagga tacagttcta ccttctgttt
     1501 gattacaatt tcggcagaaa gtttctcttt aatatcctga taaaccgttg ataaatcaat
     1561 tccattttgg tcaatcttat tgataaaaaa gattgtggga atccccattt tcctaagtgc
     1621 atgaaataat atacgagttt gtgcttgtac gccatctttt gcagaaatca gtagaattgc
     1681 cccatctaaa actgataatg aacgatatac ttctgctaag aaatccatat gtcctggcgt
     1741 gtctatgatg ttcaccttcg tattttccca ctgaaaagag gttattcctg tctgaattgt
     1801 aattcctctc tgacgttcta aaagcgtatt atccgtcctc gttgtacctt tgtccacgct
     1861 tcctaattct gtaatcgctc cactgttata taataagctt tctgttaagg tagtttttcc
     1921 cgcatcaaca tgagctaaaa ctccaatatt aataattttc atgtgatttt cctccattca
     1981 aaagcccaaa agggcataaa aatcccagtg ataaatactc ttatcactgg gatttttatg
     2041 cataaccata ggcatacaaa gcatacagat attctccgga tactttagaa tcacatgata
     2101 aaggtattct taaactgggt acaaaaaact aagccctcct aaaaaaggac atccaattat
     2161 ttgttcccac tatcaaattg acagtttatt taagaatacc ttgccgcata tttattaact
     2221 ccttttaaat agatacttaa ataatagcac gtaagagcat atttgtaaag gaatctccaa
     2281 ttttttatca aagagagtac gtgattacaa aatagctgta ataatgtacc aatatttgtt
     2341 attctataat cttccaatta ctcccgttct tttcaagtac caaatcaaat tgagatacct
     2401 gcgttgcctt tgcctgattt accacaaatc agattttatc cctttcttcc tcttgattgc
     2461 ccacaacaga aaagaaaaag ccctgtcaag agctttgcca ccattggtgg tgctttgcac
     2521 tcttgatcgg gctttttgtt tgctgtatct tctcgcaaga ggaaaaaagt tactcgtcct
     2581 catcatcaaa attgttattc cttaatactt ctcgtaacag ttccatatct tcttttgagt
     2641 tcgggtttat cattttcacg acttgataag gtgtcttata cttatgtccc tctatgcttt
     2701 ccccaaacat atccatactg tataaatcat catagcggtc taataatttt tgaaacttca
     2761 aatgctggat aaattctttg attgggtaaa ggtcggacgc ttcaatgctt ttcccgttga
     2821 tatagtctgt aatatattcc cgtaactttt ctaactcata ttccagccat tcttcctcgc
     2881 tgtcaaagaa gttatcatag tgtccatgtt tcagttctgc atttagacgt tcttcggctc
     2941 ttaaaaactg acagacttcc ttttcagatt gattgacata cttccattct cccgacaaca
     3001 attcattggg cgtaacgtcc agcacttcca ttatcttttc cagcgtttca taggcaggat
     3061 agttcaaccc tctttcaatc ttggaaaggc tctgcatatt gataccgatt ttgtccgcaa
     3121 gttcctgttg tttcattccc ctaaattttc ttatggtctg tatattattt cctaaatggc
     3181 ttattttcat tgcataaccc tccatatctc gttatatctt cgctattata agtctatcat
     3241 gcttaatatg atttgtaaag gcaaaaaaag atattgacaa ataagcatat aagaattact
     3301 atgttcttgt aatcgaataa gcacaataga atgaaatata aaaacattca aaaagaaata
     3361 cctatctgat taaatccgta gatatgaata tcggttgaaa tggaacttgt tagaaaaggc
     3421 aaaacagaac ttgctgacaa agtatttctg acacggtggc ggaagcctct caggcttacc
     3481 gccatgacac cttgtgtcaa ggggaagaaa tgctttgtcg gctggcgagc ctgcgagcct
     3541 tgtctgtgcc cccgttatct aacagggggg cactatttca caaa
//
LOCUS       DBNICI010000202         3568 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.206, whole genome shotgun sequence.
ACCESSION   DBNICI010000202 DBNICI010000000
VERSION     DBNICI010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.206"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..1579)
                     /gene="mdcA"
                     /locus_tag="AC1ZCC_002643"
     CDS             complement(<1..1579)
                     /gene="mdcA"
                     /locus_tag="AC1ZCC_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HIQ1630853.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQT"
     gene            complement(1586..2497)
                     /locus_tag="AC1ZCC_002644"
     CDS             complement(1586..2497)
                     /locus_tag="AC1ZCC_002644"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIQ1630854.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            2617..3474
                     /locus_tag="AC1ZCC_002645"
     CDS             2617..3474
                     /locus_tag="AC1ZCC_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIQ1630855.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
BASE COUNT         1003 a          775 c          648 g         1142 t
ORIGIN      
        1 tggtttgtgc agctaataat gaacgtttcg cttcactacg tttgatgcct agatcttctg
       61 gtaatttcac gatgccggct gcacgcaagc tatctagtga ttctttcgtg ctagtcattc
      121 caagaggtgt aacaccgcca attgctgcaa ttgctgcttg tcgttcttct tttgtttttg
      181 ctttgtacaa ataagcaatt ccttcttctg tcacgatatg tgtagtatct tcgctataaa
      241 tcatcactgg tgcattggct aagcctgctt gtttttgaac agctagtgca tctaattctt
      301 cgacaaagac tggtttttta tttgaaccat atgtttcaac catttgaaca actaattttt
      361 ggcctttccc taatggatca tcattgtcgc gaagtgattg ccatgctggt gtagaatgac
      421 gacgaccgcc aggattatgt cccatgttag gcgcaccgcc aaaaccagat aaacgtcctt
      481 ttgttacggt agaagagttt ccttcgtaat ctaattgcaa ggttgaaccg ataaacatat
      541 cgatggcata ttgtcctgca gcttgtgata atgttcggtt actgcgcatc gtgccgtctt
      601 taccgacaaa aaagacatct gggcgagcag caatatattt ttccatacca acttcgccac
      661 caaaactatg gatgctttca acccaacctg attcgatggc tggaatcatc gttggatgtg
      721 ggttcaatac ccagttcttc gcgattttcc cttttaaccc tagtgattca ccataagttg
      781 gcagtaacaa ttcgatagct gctgtattga atccaatacc gtgattcaaa gattgtacgc
      841 cgtgttctgc gtagattcct ttgattgcca tcattgccat tagaatttgc aattctgtga
      901 tgttttgagg atctcgtgtg aataaagctt ctaattgata aggtttatct gctttcacga
      961 caacgtctac ccagtcacca gggatatcta cacgtggtaa agaatcgaca acttcgttaa
     1021 cttgaacgat cacgatacca tctttgaaag ctgcagcttc gatgattgtt ggtgtttctt
     1081 ccgtattgaa acctgtgtac aagttgccgt ctttatctgc tttatctgca gctactaaaa
     1141 caacatttgg aatcaaatcg atgaataagc gtccataaag ttccaaataa gtatggatat
     1201 cacctaattt cattgtgccg tcttcaatca tttgggcgac acgcaagctt tgtgcaccag
     1261 cataagagaa atcgatcttg cttgcgattc ctttttcaaa aatattcaaa tgttctggtc
     1321 gtgagatact agacatgatc aaatgcagat cattcacttt ttctgcatct actttttcaa
     1381 gactttctga aagaaaactt gcttgttttt gattgtcgcc ttctaataca actttgtcgc
     1441 cgggagcaat cagtttttcc attacttcta cgattttttc agtttctaca tacttgccat
     1501 tgagcaagtt gctgattttt tccaatcgct gcgctttttt ttcgcgttta gaagcccagt
     1561 tgcggtttgg tttttccata attagtcatc tccatttttg atatagaaac gtggcgtcat
     1621 caataccatg ttggtctaga ttgatcgtac ggatcccttt atctacacca aattttgtat
     1681 gaatcagctt tgtcaaagta ttgccggggg gaaattcaat cgtcagatcc attcctaatt
     1741 cttttgccaa gtccatcatt tggttccact gcacagggaa agctaggttt tccaacaaat
     1801 ctttacgaat ctcttctgcg tcctgagtgg ctcttccatc cgtatttttt atatacaaac
     1861 atctagcatc agatagatgg acagactgca tatacggctt gaatttcttg accgtatcat
     1921 tcatcaagag acagtgagat ggcacaggga ctttcagcag cttcgctgtt tgcgcttggt
     1981 gccgtttggc ttctgccaac gtaacggata aaccttcgat actcccagat aacgtatgtt
     2041 gcatcggaca attttcattt gacagataaa ctggatggtc aggatcaaat gtttttgcta
     2101 caatttcctc agcttcgctt ctcgtcacac ctactactac gcccattgca taaccgcttg
     2161 gataagcttc tttcattaag actgcgcggt gatagactag ttccgctgca ttctcaaatc
     2221 tcaaagatcg acaagcaaca gctgcacaaa aagcaccaat cgaatgtcct gctgcaatat
     2281 caggctgaat accgtttttt tccatttcat ctaaataaaa tactgctttt gtcagtaatg
     2341 ctagctggat aaaaaccgta tcttgatagt tttcttcttt atcaactaac cgataccccg
     2401 tcacattttc taaacgttcc atgtacattt cagggatatc cgctaacatc cctaacttct
     2461 gactgccttg tccaggaaat aagaaacctg tactcatgag cctcgcctcc tcagcactta
     2521 ctttttccac tgctatctta gtcaatttct gctataataa aaaatgccaa aaaaagaaaa
     2581 aaccataaca aaaaggtatg gatgggatgg ataattatga aattgcttca tctgcagtat
     2641 tttaaaaaag tattcgaaac aaaaaatgtt acccaagcag ccaaagaatt atttatttct
     2701 caacctgcct tgagccgtgc gatcaaacat ttggaaaacg aattaggtgt ccctttgttt
     2761 taccatagcg gcagaaacat tgagacgacc gtttatgcag aagaatttta tccatatgca
     2821 gtcaagacat tagacacatt agaagaagga atcaaattat tgtcttcggt caatgagaag
     2881 atcgttactt ccgtcatatt gtatttagag gtagcctctg tttctattcc gaacttggtc
     2941 cgaattttta tggaaaagca tcctgatatc caattaacga tcatgcaaca tcatttacca
     3001 gatgatatta ccgatcctgt actgtacatc acttcggaga tgaagcctgg catgaccaat
     3061 attcctatca tgaaagaacc tgtttatgtc gccattccta agaaacatcc tcttgctaaa
     3121 aaagaacatc tgcaattaga agatatccaa cgcaccccca tcttgatgct gtcaaagagc
     3181 aacgccttcc gtcatacact ggatgcagca cttgaagcga aaagtatcga attgacgatc
     3241 agttcgacga cagatgatcc tgcgacatta cgttctattg tcaagcaagg attagctgtt
     3301 agctttttcc caaaagtttc ttggtcttac gataaaaatg atcctttcgt cttgcgggaa
     3361 atcaaagatt tgcccttgac acgaacaatt tatttatctt ctagcttcac agaagatcat
     3421 cctttgatca aaatgattgc aaaaacgatg cgggaatttt atttaggaaa ataaaaaaaa
     3481 aagagctggg acaaaattct tgtcgcagct cttttttcga acaaacggtg ttccagaagt
     3541 aatttcttag aaaataagcc gaaatttc
//
LOCUS       DBNICI010000203         3554 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.207, whole genome shotgun sequence.
ACCESSION   DBNICI010000203 DBNICI010000000
VERSION     DBNICI010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.207"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..524)
                     /locus_tag="AC1ZCC_002646"
     CDS             complement(<1..524)
                     /locus_tag="AC1ZCC_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIQ1630856.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIITSVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNG"
     gene            838..1260
                     /locus_tag="AC1ZCC_002647"
     CDS             838..1260
                     /locus_tag="AC1ZCC_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630857.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1270..1473
                     /locus_tag="AC1ZCC_002648"
     CDS             1270..1473
                     /locus_tag="AC1ZCC_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630858.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            1684..2268
                     /locus_tag="AC1ZCC_002649"
     CDS             1684..2268
                     /locus_tag="AC1ZCC_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIQ1630859.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            2437..>3554
                     /locus_tag="AC1ZCC_002650"
     CDS             2437..>3554
                     /locus_tag="AC1ZCC_002650"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HIQ1630860.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYT"
BASE COUNT         1203 a          561 c          676 g         1114 t
ORIGIN      
        1 ccatttttaa gatcttcaac gatgcttttg taaacaaggc gacgttgggg atcttttgcg
       61 tctgcactgt ataatttggg ccgccctttg tagacgccgt ttgcttttgc gatcttaatt
      121 ccttgtgcct ggcgacgttt cgattcagtc cgttcttgtt cagcgatcat ggccaaaatt
      181 tggatgatca gatccttaat gaatttatcc aggagaggat tgccaattgc ttcagccatg
      241 atcggtaaac tagtaatgat cagcttcaca tttttcttct tcaagtaatt gactgaagtg
      301 atgatctcgt cataatttct tccaagtcga tcaatagctt ccacaataaa aatatcttga
      361 tctcggacgt aatctaatgc ttcttggaaa acaggtcgat gagtaatcgt tgcgccagat
      421 tgtttctcga tgaaaattct ctctgcaccg aattttttca tttcttcaat ctggcgttgt
      481 tcattttgat cgatcgatga gacacgtatg tatgcaactc tcaaaacttt tacctacttt
      541 ctgcaactat ttaaaataca ccctaatcat acacgaggaa gccatatttt tcaatgttat
      601 accagtgtct atttagggta aaccttatat agacaaaaac taatgatgga ggaaatagta
      661 tgtgtcactt ttctttttaa atggcaatat tttttgtaaa taaatgtcaa tcaagaaaat
      721 ttaaacgatt atgacattaa aaatatattt tatgaatttt ctagtatata acttgattta
      781 tctgttaaga tttattaaga aaacgttttc ttatttttga tttcgaggtg atagacaatg
      841 aaaaataagt ttattttatc tttaggtatt ttaactattg gaattagtgt aactggaact
      901 acaattccta catttgctga tagtacaaat gaaccaacta ctgtagtaga taataaccaa
      961 gaattaactc ctttagatag gaacggaata gccattggta acccaactaa cgacttaact
     1021 gttactgaaa attatttaat agtttctcca ttacttgctc gagcagcttc taaaacgata
     1081 cgagtgacag gaacgatgat ttttgataaa gttggttggc cttctagacc tcctcaatca
     1141 cggaattgga aagaaaaaag aaatggttat tggtactatg gaaaattgta tttacaaagt
     1201 tataaatcag ctggaaacaa ttggattgcg acttattcag gaaccttaac tagaaagtag
     1261 ggatttttta tgccagaatt tgtgaacaca ccaaattttt atcaagatag cgatattcta
     1321 agtaaaatta atttaccgaa agaagaatca gctttacaag ttcagcgaac tttacaggag
     1381 acacaaaaaa ttcaaaaatc aatttctaat ctagaagatt ctgaagaaaa aagaaaaaaa
     1441 agagaccaaa aaacatatca aaaagaaaaa taatgatttt aaaaaaagag tgtctagtcg
     1501 acattctttt tttctaattg aaagtgttca taaaatcacc catttctgga cactttcttt
     1561 ttttgcttta tttttcaaaa aaacaattca tttcatgttc ataaacccat aacaatatct
     1621 atgttcagtt aaactatatg gataaagtta tgttacaata aaattaatga aggagtgatt
     1681 tttttgaaaa taggatacgc ccgggtatct actggtttac aaaatttgga tttacaagaa
     1741 gacagcctaa ataaatttgg ttgtgaaaaa atttttactg atcatatgag tggggcaaaa
     1801 agtaatcgac caggattaga aatggccatt gattttgttc gctctggcga tacacttgtc
     1861 gtttggcgct tagatcgttt aggtagaaat atggaagatt tgatttcaat cgtcaatcga
     1921 ctgaatgagc gtggtgtgag ttttcatagt attcaagaaa atattacgat ggacaaatca
     1981 agttctacag gacagctcat gtttcatttg tttgcggcat ttgcagaatt tgagcgtaat
     2041 ttgatcctgg aacgatccgc agcaggacga gcagctgctc gtgcaagagg acggtttggt
     2101 ggtcgtcctg aaaaattgtc tgaacaagat ttggaattac tgaagacttt agtggatagc
     2161 ggtacaccta ttaagactat tgcggaacgg tggaacgtct caagaaccac catttatcgt
     2221 tatttggcaa aaatggagga gaaaaatgga aagacaaatt ctcattagtt tttgtcattt
     2281 aatgctgaaa atttttgcca tttatctaga aaagtgacaa tagtatgaga attttttgta
     2341 agtgtctact gataacttat tctcaaatat ttagctattt cttctttaca acatcgaaca
     2401 tatgtttgta taatgtggaa gaatggagtg ttatttatga tggattattc aaaagagcca
     2461 gtaaatgatt actttttaat cgatatgaaa agcttttacg caagtgtcga atgtatagaa
     2521 agaaatttag atccattaac aacagaactt gttgttatga gtcgatctga taatactggt
     2581 tcaggattga tattagcttc ttctcctgaa gcaaaaaagc ggtatggtat tacaaatgtg
     2641 agtagaccac gtgacttacc acaaccattt cctaaaacac tacacgttgt tccaccacgt
     2701 atgaacctat atatcaagcg aaatatgcag gtaaataata ttttcagaag atatgtggct
     2761 gatgaagatc tactgattta ctcgatcgat gaatcgattt taaaagtgac aaaatcactg
     2821 aatcttttta cgactgaaga aacacgaagt cagcgaagga agaagctcgc tcaaatgatc
     2881 caagaacgta ttaaagaaga gttaggtttg attgctacgg taggcgtcgg cgataatccc
     2941 cttttggcta aattagcctt agacaatgaa gcaaagcata atgaaggatt tattgcggaa
     3001 tggacgtacg aaaacgttcc tgagaaagta tggaatattc ccgaaatgac ggacttttgg
     3061 ggaattggtt cgcgaatgaa aaaaaggctc aaccaaatgg gaattttgag cattagagac
     3121 cttgctaact ggaatcctta tacgattaag aatagattag gtgtgattgg attacagctg
     3181 tacttccatg ctaatgggat cgatcgaaca gatatagcaa tcccaccaga accgacaaag
     3241 gaaaaatcat atggaaattc acaagtgtta ccacgagatt atactagaag aaatgaaatc
     3301 gagctggtgg taaaagaaat ggcggaacaa gtagcgatac gcattaggca gcataactgt
     3361 aaaacaggtt gtgtccattt acacattggc acgtcgatct tagaaacgag accagggttt
     3421 tcacaccaga tgaagatccc tatcacagat aatacaaagg aattgcagaa ttattgtctt
     3481 ttcttgtttg ataagtatta tgaaggacaa gaagtaaggc atgttggaat aacctatagc
     3541 aagcttgtgt acac
//
LOCUS       DBNICI010000204         3441 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.208, whole genome shotgun sequence.
ACCESSION   DBNICI010000204 DBNICI010000000
VERSION     DBNICI010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.208"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(6..1220)
                     /locus_tag="AC1ZCC_002651"
     CDS             complement(6..1220)
                     /locus_tag="AC1ZCC_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIQ1630861.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(1306..1533)
                     /locus_tag="AC1ZCC_002652"
     CDS             complement(1306..1533)
                     /locus_tag="AC1ZCC_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630862.1"
                     /translation="MKNKFENRSDTRCGSFLYILYEVFFIPYEKISLKIKKDENKRFS
                     IKRRPFIFCLYLCEKRKRAQHIKLLLIVCLL"
     gene            complement(1561..1803)
                     /locus_tag="AC1ZCC_002653"
     CDS             complement(1561..1803)
                     /locus_tag="AC1ZCC_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIQ1630863.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(1835..2392)
                     /locus_tag="AC1ZCC_002654"
     CDS             complement(1835..2392)
                     /locus_tag="AC1ZCC_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIQ1630864.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(2405..2593)
                     /locus_tag="AC1ZCC_002655"
     CDS             complement(2405..2593)
                     /locus_tag="AC1ZCC_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HIQ1630865.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(2606..3172)
                     /gene="amaP"
                     /locus_tag="AC1ZCC_002656"
     CDS             complement(2606..3172)
                     /gene="amaP"
                     /locus_tag="AC1ZCC_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HIQ1630866.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         1016 a          727 c          558 g         1140 t
ORIGIN      
        1 ttgtttcaat tgagtttgtt gccaaagaag aaaaagtcca atccttcttc ttgttcttct
       61 tttgtagtag tgatcgttgt aaagtgcatc gccgatttta aaatcactgt tgtcagcaca
      121 taggcataca cggcagtaat gatgacacca atcacttgaa tccctaagaa gtaccagctg
      181 cttggattcg ttagtaaatg gctgttagat gcaaagatcc ctatcagaat cgtacctgtg
      241 aatccgccca tgccatgaac accccacaca tcaagtgcat catcccattg tttcttttta
      301 cgaaaatcaa cagcagcatg acagacgata cccgcaatga tcccaatcag catggcactt
      361 tttgcttcta cataaccggc acaaggcgta atggctgcca atccagcaac ggatcctgtt
      421 agtacgtcga caaaatcaaa gccatttcct tttacttttg caatgatcag ccaagtaatc
      481 atcgcaaatg caagagcgat gaatgttgaa ccaaatgcat tgactgcttg ttcatctgct
      541 cgaagtgcac caccggaatt aaaaccgaac cagccaaacc aaagcagtcc tgtaccgata
      601 gcagctgcca taagactgct atgctgactt ctttctccta aaggaatctt tctttgacca
      661 agtgtgagga tacatgctaa agagccgaat ccagctgtcg tgtggatgac tgctccacca
      721 gcgaaatctc taaatcccat ctgatccaaa aatccgccac cccatatcca gtggcaaaca
      781 gggaaataaa tcagcagatt ccaaaaaata acaagtataa tatagccttt taaattcaat
      841 ctccctgcaa aagctcctgt catcaaagga acggtaatca cgataaacat cagttggtaa
      901 agaaagaaca ttaaaaatgg aatcgttgca ccatgaaaca tgttggggaa gcttgtaaca
      961 tgtctcatca aaaagaaatc tgccgggttt ccaataacac cgccgatatc ccgtccaaag
     1021 gccaatccga atccaccaaa tacccacata atcgttgtta tcccaacgga gagaaaagat
     1081 tgagacatga tcgttagtat attattcttc cgtgctaatc cgccgtaaaa aaaagctaga
     1141 ccaggagtca tcaggcaaac cattgctgta cataaaatca tgaatgctgt atctcctgta
     1201 ttgataggta atgtattcat ttttttcgcc tcctttctac gattacgagt ataaaagaag
     1261 gtgtagttgt ttttttcttc tgtttttttg aattaatact tgcatttata aaagacagac
     1321 aattaataaa agcttgatgt gttgtgctct ttttcgtttt tcacacaaat aaagacaaaa
     1381 aataaacggt cttcttttta tagaaaaccg tttattttcg tcttttttta tttttaaaga
     1441 tattttttca taaggaataa agaatacttc atataaaata tataaaaaag aaccgcagcg
     1501 agtgtcgcta cggttctcaa atttgttctt catttcaaca taagtttttc ttcaatttgc
     1561 ttataattta cgtgcaaaga atgaaacgac taatactaaa atgattgcac cgatcaatga
     1621 tggaatgata gccattcctg caattgttgg tccccaagtt cctagtaaag cttgtccaat
     1681 tgctgaacct actaaaccag caatgatgtt tgcgatgatc cccattgatg atcctttatt
     1741 cgtgattgct cctgcaattg ctccaatgat accgccgacg attaatgccc aaataaatcc
     1801 cattttaaat tcctcctaac tgacttctta cttcttattg tacacgtggt tcgttatttt
     1861 ctttgacttt ttgtgcacct ttgcctacag cagattttgc tttatctgtt tggcgtgaag
     1921 caaattgtcc tgtggatttt gctgcttctg ttactttgtc ttgtactgtt tcttgatctt
     1981 tttggtattc ttcttgtgtt ttgatgtctg caacattcac attcacttcg ataacatcta
     2041 aatgtgtcat attgtttact tcagtactga tcaattcttt gatttggttg taaatatctt
     2101 cgatgtcttt accgtattct acgatcacat ctagatccac agctacttgt ttcttaccta
     2161 cttctgtatt gatacctgct gttacgttat ctgtgttgac taatttttca gccatatttg
     2221 agaaaaatcc gccatctaat gtcaatagac catctacatc tttcaatgca taaccgatga
     2281 ttttttggat gactttgtct tcataagtca attcaccatt catcatgcgg ttttctcctt
     2341 tttcatttgc caaaactgat ccatttgttg tattcatgtt tttgttttcc atctttgttt
     2401 cctcctatga tttaaaaaat tgatcaacta ttcctgactg tttcaaataa aaaccgattc
     2461 ctgcgccaat tgctgtcatg attatgatca acaatgtctt gaaaaatcca atcgttaacc
     2521 ataaaacagc tagcagtagt ccaagacccc caaagataat agggagcttg tatgctgaaa
     2581 atagttcttt cattatttgg ccctcctatt ccactcgaga gtgcttgtag tctcttgtat
     2641 cttcttgttc ttcataaccg ctgtatgtga ctgccacttt gaccgtttct tttgttccga
     2701 gtactttagt tacttcttct tcgatatctt gcatcaatac acctgttttc cctactaatg
     2761 aagaagttcg tttcaagtct ccgttgacat ggatatggat tcgatttttc gttgaacgta
     2821 tccgtacttt aggtgaatga atgaactctt cttcgtgtaa atgggaacgt accaaccctt
     2881 cgatcgcttt tttgtcgatt gttagtttgc catcttcccg tttcataacg aaagttcctc
     2941 ttgactttgg atagaaaaga acgactagca agatgataat agcaagaacg gcaaatacta
     3001 cagctgccca gaaaatatat tgttgcatgt agtaattcgt taaagtaaag aagcgaaatc
     3061 gttccaattg ttcaggcata atatacggcg cattgatcaa cgcaatcgct agaaatactg
     3121 aaagtagcag taaactcatg ataacaccga ttatttttaa ccccttattc attttccacc
     3181 tcctataaac ttatttttac cctgtaaata actctaccga caatcacagt atagatagcg
     3241 aacagttagc atagcatcag taaataaagc gctaccaaac attgctggat cataacggcg
     3301 ttttattgcc agtctgatac tgaaataaca attgcttact tattctctat ttacttttga
     3361 ttgctggcta tacttaaagg atattattca agattaaaaa ttgcaaagat tatgctcagt
     3421 tattattaca agattctatt t
//
LOCUS       DBNICI010000205         3417 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.209, whole genome shotgun sequence.
ACCESSION   DBNICI010000205 DBNICI010000000
VERSION     DBNICI010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.209"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..203
                     /locus_tag="AC1ZCC_002657"
     CDS             <1..203
                     /locus_tag="AC1ZCC_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HIQ1630867.1"
                     /translation="DKRTENQVRGMIEWSQHNVFWASNILSAKKLREKYDTMAAQANR
                     DYKTKQTKTLEYEKFSTDELPI"
     gene            218..1069
                     /locus_tag="AC1ZCC_002658"
     CDS             218..1069
                     /locus_tag="AC1ZCC_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIQ1630868.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            1270..1731
                     /locus_tag="AC1ZCC_002659"
     CDS             1270..1731
                     /locus_tag="AC1ZCC_002659"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIQ1630869.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            1757..2209
                     /locus_tag="AC1ZCC_002660"
     CDS             1757..2209
                     /locus_tag="AC1ZCC_002660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630870.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            2315..2509
                     /locus_tag="AC1ZCC_002661"
     CDS             2315..2509
                     /locus_tag="AC1ZCC_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630871.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            2540..2833
                     /locus_tag="AC1ZCC_002662"
     CDS             2540..2833
                     /locus_tag="AC1ZCC_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630872.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            2764..3303
                     /locus_tag="AC1ZCC_002663"
     CDS             2764..3303
                     /locus_tag="AC1ZCC_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HIQ1630873.1"
                     /translation="MVSEKQREFSSGEIGGHGSYKILILLFYNYPTTMSVIPQLEGEE
                     AIVNILENIDIKQTRKNARRLLKRYRNLERLVGPVKIDFSVMTVTKNLKFTIDSQNEE
                     IIEAISTRDSVIEALTRLSRIHFQVLYYSYCFPNKMSMYQIGEKLGYSDRTIERMKAV
                     ALVEFAEAYKSGELISRTK"
BASE COUNT         1210 a          533 c          784 g          890 t
ORIGIN      
        1 tcgacaagcg gaccgaaaac caggtacggg gaatgattga atggagtcag cacaatgtgt
       61 tttgggcatc gaatatttta tctgctaaaa aattgcgaga aaaatacgac acaatggcag
      121 cgcaagcaaa tcgtgattat aaaacaaaac aaactaaaac gcttgaatac gagaaattta
      181 gcacagatga gttgcctatt tgagaggagg cgtaggcatg gaaactgttg gggaaatcat
      241 ggaaaagctg atacagaaag tgcttgtcca acgtggcgaa tgtcctgaat gcggacagcc
      301 tttgtatgga tggcgtacga agaaccctga tggctcagaa cgttgtaagc caacctgtat
      361 gcaatgtggg tataaagcat tgcgagttca ggaagactta caaacagaac gaatttacaa
      421 cgagagcctg aaagcaagag caatcaattt tttcaaaggt ggttctgttg tgcctaatca
      481 agcgttgttt gattgcacat tgcagaatta tcaaattgtc gatcaagaaa caagacaagc
      541 ggttgaagta accaaacgct ttgttaattc agtcttgtta ggaaatccaa gtcaccttgt
      601 tttaactgga aaacaaggaa caggtaaaag tcacctagcg atggcagcgg cttgggaagt
      661 cttgaagcga tcaaactacg ataaaaaaat cttatttatc gggttacagg aaatgctgga
      721 tcaaatcaaa ttttcgtaca acaatcctga actcagaaaa acgattgagg gatcgttgat
      781 tgcagatatc aaaacagcgg atttagtcat cattgatgat atcggttcag aactaggaaa
      841 agatgcatca gatagtcgag cgtttggcat aaacacgcta aattcgttct tggatgcacg
      901 acagaaccta gcaacgatta tcacaacgaa cttgcttggt gaagaactga aaaaagctta
      961 cggtacgaga acaatatcaa gaatgtttgt caactctgat ggatttacga tggtattttc
     1021 tcaaacagca gacaagcgca taaaaccagt caaaggagcg atcgcatgaa tttgactaaa
     1081 caagaaaaaa gaatctgtcg tcagataatg ttgcaaatta taggtgatcg tgacatggac
     1141 gaattatctc aacaagagag tcgaatggtt cggaagataa gtaagctaat tatttatcgg
     1201 gatacagaaa gatttctcac tgttttaggt aacgtctacg agaatccaga gttattggag
     1261 gtagagtaat tgccaaaaat attagatgca tgttgcggta gtagattgtt ctggtttgat
     1321 aagaacaatt ctgacgtaac atttatggat tgtcgtcagc agtatgaaga attatcaaca
     1381 ggtcatgtga tcaatgttga tccagatgtg gtcgcagact ttcgcgatat gccttttgaa
     1441 gatgatgtgt tcgatatggt tgtatttgac ccgccacact taagacatgt cggcgaaaac
     1501 tcatggcttg ccaaaaaata tggaaaatta gacgaacttt ggccagaaga catcaggcaa
     1561 ggttttgcag agtgtatgcg agttttacga ccttcaggaa cattaatttt taagtggaac
     1621 gaggaacaga taccactttc tgatgtgtta gaggctattg gagaacagcc attgtttggt
     1681 aataaacgaa gcaaaacaca ttggcttgtg tttatgaaaa aattcagcta acggaggaaa
     1741 caaaggagga aaagtaatga agattgtatt aaataaatgt tttggtggct ttgggttgag
     1801 ccatgaagca aaaatggaaa tttttaaaag aaaaaatatt gaagtattcc catacatcaa
     1861 taactttagc tatgactcag atgatgaata cacgagatac acaggccaaa agctaggttc
     1921 gatggatttt atctactatt tcaaaaaaga cccgaaaatc gacaaagtta caggaattta
     1981 tagcgaaatt gaccgactgt acggaattgc tgatgattcc agtttttcaa gtgattcaaa
     2041 cagaggtgat aaggacctcg ttgctgttgt cgaaaaacta ggaagtgagg ccagtggacc
     2101 atatgcaagt cttaaagtcg ttgatatacc cgatggggct gagtgggaaa taagtgatta
     2161 tgacggcgtt gaaactgcac attatggttt tcaaacaggt agtatttagt ccactaatga
     2221 caactaaaga ggaggcagaa ggatgagtga agatgaatac tttgaacagt agtctattaa
     2281 cgacagagat ttttaactgg aaaggggtgt agtcatggac gcatcggaag tagtaaataa
     2341 aattaatagt ctcaaagagg ttttcggaga cgtcgaagta gtgataaacg cacaaggcta
     2401 tgacaacgaa ttttttaagc agattaccga cattagcatt cagcaaggaa tggaagacga
     2461 agacggaaac tttatcgatg aagtagccat tttagtgact tgcgagtaag taattgacag
     2521 ctaaagagga ggaagcggaa tgaaactaaa agacggattt tactccagcg gccacggcat
     2581 cggcggttta atgctagata tgccgacaaa gaatcctaaa acacgtaaga aaccaaaatt
     2641 caaagtcggt gacatggttc gctgcgaagc agaaggattc atctatccat ttcgtggata
     2701 tgtagaacac gtctataatc actcagcaat cattcgcatt gaaaacacga tggaatgtga
     2761 taagtggtta gcgaaaagca aagagaattt agcagtggtg agattggtgg acatggaagt
     2821 tataaaatct taattttgct tttttacaac tatcctacaa caatgagcgt tattccacaa
     2881 ctggaaggag aagaggcaat tgtgaacatt ttagagaata tagatattaa gcaaacaagg
     2941 aaaaatgcta gacgattact aaaaagatac agaaatttag aacgtttggt cggaccagtg
     3001 aaaatagact tttccgtgat gactgttacc aaaaatttga aattcacaat tgacagtcaa
     3061 aacgaagaaa tcattgaagc gataagtact cgagattcgg ttatcgaagc attaacacgg
     3121 ctaagtagaa tccatttcca ggtactttat tatagttatt gttttcctaa taagatgtca
     3181 atgtatcaaa taggagaaaa attaggatat tccgatagaa ctatcgagag aatgaaggca
     3241 gtagctttgg ttgaatttgc tgaggcgtat aaatcaggag aactcatttc acgtacaaaa
     3301 taaaaaaagt cggattcctc cgactatgaa taatatttcc gacataagta ttatatcata
     3361 attgggggaa tcagaggatg gtacttttcg atgtaaagaa atatgagaca ccaagcg
//
LOCUS       DBNICI010000206         3406 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.210, whole genome shotgun sequence.
ACCESSION   DBNICI010000206 DBNICI010000000
VERSION     DBNICI010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.210"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            284..610
                     /locus_tag="AC1ZCC_002664"
     CDS             284..610
                     /locus_tag="AC1ZCC_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630874.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(727..1071)
                     /locus_tag="AC1ZCC_002665"
     CDS             complement(727..1071)
                     /locus_tag="AC1ZCC_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630875.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(1147..2394)
                     /locus_tag="AC1ZCC_002666"
     CDS             complement(1147..2394)
                     /locus_tag="AC1ZCC_002666"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HIQ1630876.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            complement(2391..3197)
                     /gene="proB"
                     /locus_tag="AC1ZCC_002667"
     CDS             complement(2391..3197)
                     /gene="proB"
                     /locus_tag="AC1ZCC_002667"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIQ1630877.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
BASE COUNT          997 a          672 c          679 g         1058 t
ORIGIN      
        1 agctaatacc gagggtcagc tgattgaacg ccgtttacca agaggttgtg agactgtcgt
       61 ttaactctga atcacaagga acaatttcaa aaaacagttt ctcatgtttg ttgaatattg
      121 tgacttatga ttgaggagtt ggcagaagaa caccatttat tcggaaaaag gggatgtgac
      181 acgatttttg tcacattccc tttttttctc aaaataatga aactggaaaa ttttcagtag
      241 gtaagctaaa attcatttaa gaaaaagaat ggggggataa tagatgctgg aagaatattt
      301 tcaggaagtc gggttgacac tcgttgaatt acccattgaa ttttcagatg atcggttgat
      361 ttatcgtggc tatatcaaag aattgccaat aatcaatgcg gaaggtgaaa ctagaaaagc
      421 gatgcagcaa aaaatagctg gtatgtatca ggtgtatcgg gaacagctac tggctgaatc
      481 agcggaagaa gaaaaagaaa aaacgatgaa cttgtccatg gatgagctgc ttcgttatta
      541 tgatggcgaa acatttgacg gattttcgct tcttaacgaa gaggaaacag atttaccagg
      601 cagctggtag gcgaacgccg tttatatgga aaaagaggct gtgacaaaat ctttgtcaca
      661 gcctctaaaa agtccggcag ttttttgccg atcttcaagg cactctactt atcgtacgga
      721 tattttttag cggaaagtcc gattcaagtg gtttttattc agctgatatt tataagataa
      781 gtgaaaaatc ccagcaatca gtagcgtaat cgtataagcg attgaatgat cgaaaaaaga
      841 atagtaattg attgtcgcta ataagcataa tgcaaccatg taactaatgt gcaagcgtgt
      901 aaccagccct ttttgcccga ttttgtcaaa atactgcgac catttttccc gttgactccc
      961 tatgcttcgt tttttgaatt gaaagaaaag ggataaaata ccgactccca gaaaagcagc
     1021 gatattcaga gaaataaggt aaataaaaaa aatcattaaa atttcaaaca tattgacacc
     1081 tttctagttg gatagaacct ttacagaaaa aaggcaaaag gcgggaataa tgtttcacac
     1141 gaaacattat tcgcggattt gtccatcgcc ataaatgata tattttgtgg aagttaattc
     1201 atttaacccc attggaccac gggcatgcaa cttttgagta gagataccaa tctctgcacc
     1261 aaaaccaaat accgatccgt ctgtaaaacg agtggaggca ttgacataga cagccgccgc
     1321 gtccacttca tttaagaaac gttgactagc cgcataacta tctgtcacga tcgtttctga
     1381 atgtttagta ttatgccgat tgatatgtgc aattgcttca tccaaagaat cgaccacttt
     1441 gacagcaagg atataatcta gatattcagt atcccaatct gtgtcttctg ctaaagcagc
     1501 cgtctctaga tattctaacg cacgagcatc cgcacgtaaa gagaccccgt gttctaccag
     1561 ttctttttca atagctggca agaaaaaagg tgcgatctca gaatggatca gcaatgtttc
     1621 tgccgcgtta cagacagatg gacgggaagc ttttgcattg acgatgatgt tcactgccat
     1681 agataactgt gcttctttat cgatatatac gtgattattt cctgtacctg tttcaatgat
     1741 aggtaccgtt gcctgttctt tgactcgttg gattaatttt gctccgcctc gaggaatcag
     1801 cacatctaag tattctgtca atcgcatcaa ctgtcctgca gtttcatgag aagtatcatc
     1861 gacaaactgg atggcataag gagattcgcc agcactgacc agtacttgct gcaataattg
     1921 gactaataat tgattcgaga agaatgcttc tttgccaccg cgaaggataa cggcattccc
     1981 tgatttgaat gctagactag ctgcatctgt cgtgacattt ggacgagatt cgtaaatgat
     2041 gccgatcacg cctagtggca cgcgcttttt gccgattctc aatccgtcgg tgttcgtcca
     2101 catctcatct acttttccaa taggatcgtc taacgaagca attttttcga tctctgtagc
     2161 catggcattg attcgctcag gtgtcagctg caagcggtct tgcatcgttt cactgatatt
     2221 gttttcgact gctttttcta agtctttatg atttgcttca atgatcgctt gttgattctt
     2281 tttgatggtt tgtgccattt gaacaagtag ctcatttttg cggtttgtag gcataagtgc
     2341 tagttgttgc gcgctatctt ttgcctgttg tcctaattgc atcaagtcag tcatttcaag
     2401 tcctccttaa ataaagtacc tacttctttt cctgcaagaa tatcaaaaat aattgccgga
     2461 ttttttccat ttgctaaaat cattgcgctg tttgcttcaa atatcctaga agcggctttt
     2521 aatttactct gcataccgcc tgttccaaat gtgctaccag caccacctgc ttgtgccaga
     2581 atcttttcat cgattttatc gatatagcga tacatctgag cctcgggatt tgtagtcgga
     2641 ttatctgaat aaaacccgtc gatatcagaa aggacgatca aaaggtctgc cgcgacaagt
     2701 tttgccatga tggctgacag ctgatcatta tcgccaaatt ttgtcagatg atctaattca
     2761 tccactgcta cagtatcgtt ttcgttcacg atagggatcg tgtccataat caaaagctgt
     2821 tccaatgtat tcctcacatt tttacgactt tcaggataat ctaccacgtc tcttgtcacg
     2881 aggatctgtg ccgtctgttg attatataca ctgaatcttt gattatagat attcatcagt
     2941 tcggcttgac caacagccgc cgcagcttgc tgttcaggaa tcaaagtcgg acgttctgac
     3001 aaatgtaatt tatgcatacc aacgcctaca gctcctgatg agactaagat cacttccttt
     3061 ccccgatttc taagatcaga aaggacaaaa gctaactgat cgattgccgc aagattgata
     3121 tttccattgg gataaatcaa tgtacttgtt cctactttga caataattcg ttttgcattt
     3181 ttaatttctt tacgcatgtg acacctcaat caaatatcca ctcattaatt tcgttctatt
     3241 ctacttgttt tgacgagtaa agtcatgtaa aaaatagttt ttttgtacaa cttgcagtcg
     3301 tttggaaaaa gaacgaatta atggtttact ttaagatagc agaatttctt gaagaatttg
     3361 taaagaaagt cttttacagc ctcggatatg ttagaatagg agtagt
//
LOCUS       DBNICI010000207         3385 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.211, whole genome shotgun sequence.
ACCESSION   DBNICI010000207 DBNICI010000000
VERSION     DBNICI010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.211"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(206..691)
                     /locus_tag="AC1ZCC_002668"
     CDS             complement(206..691)
                     /locus_tag="AC1ZCC_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HIQ1630878.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    781..1015
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1171..1812
                     /locus_tag="AC1ZCC_002669"
     CDS             1171..1812
                     /locus_tag="AC1ZCC_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIQ1630879.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1831..2466
                     /locus_tag="AC1ZCC_002670"
     CDS             1831..2466
                     /locus_tag="AC1ZCC_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIQ1630880.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2484..3299
                     /locus_tag="AC1ZCC_002671"
     CDS             2484..3299
                     /locus_tag="AC1ZCC_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIQ1630881.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT         1103 a          554 c          725 g         1003 t
ORIGIN      
        1 aaaaaagaag catttttata tatgcttctt ttttcgtaga atcaataaat atggatttgg
       61 ctaactatgg aagaatactc gtattagttt tcagcgttca tactagttgt tacattgttt
      121 gattggccaa aagaatttct gatatatttt gtgctaaata aagaaaaaga cgcttgaatt
      181 gggaaataag aaaactactc tatcattaca aattttcata aactggcaga tgttctaagt
      241 aaacaagtat cttttcaaag tcggaaaaat ccataatcaa cgtagtggta ttgatatttg
      301 gatgaaagcc tacggtatct tgacgatcaa tagcagcatc gatcacgact tggatttttt
      361 tctctttgtc gtgcatcaat gctaatggag tcactgtacc tgctggaacg cctaggaggt
      421 caggaagctg ttcttcagaa acaaaagata agcgcttttc ttttaattgt tcagctagtt
      481 gttttaaatc ggcgtgcttt tcatcaggca agatgacaag atagatttgt tttccttttc
      541 ttgatttcaa taatagattt tttacttgcg gaccaggtag ggaaaaaggg acatttttga
      601 ctgacgtaat cgcaggatga tcgacttttt gaaaagagat acccagtttt tttagtagtt
      661 cataagcttc ttgttcggtt gcacgactca tcgcgcatca ccctcctttt tttattagtg
      721 tatcataaaa aagagttgac aaatgaaaaa taatcatgta aattacacgt aaagttaaaa
      781 caaatgacat cgaaaagaag agtagccgtt aggtgttttt agagagtctc tggttggtgg
      841 aaagaggcag cagttaaccg tgaaacacat cttggagtcg attttctgaa gcgtatagta
      901 ggaaaatccg gggagcccgt tacagctgcg gtcgttgcta gatgaccaga taaggctggc
      961 ttttccgtga ggagtcggta aaaaaaggtg gaaccacgtg attaacgtcc tttgatcttc
     1021 ggatcaaagg actttttgtt ttcatctgaa cggcaacgac tgtgtgaggt tttttctgca
     1081 aggaaaaaat aaacagaggt ggaaccacgt ataatacgtc ctcttggcat ttgccaagag
     1141 gattttttta ttttaaagga ggaaaagaag atggagtatt tgattgaagt tatgccgcaa
     1201 ttattagtag gagcactcac aacattgaaa ttatttttat ttaccttgat tggctcatta
     1261 ccattaggga ttgttttagc attaggattg tctttgcatt ttaagccgct acgctacttg
     1321 cttcaaacat atgtatgggt tatgcgggga acaccgctgt tgttgcaact gatttttatt
     1381 ttttatgggt tgccgacgat tgggatcgtt tttgatcgtt tcgaagcagc actcattgcc
     1441 tttatcttaa actatgcagc ttattttgca gagatctttc gtggagggat acaatcgatt
     1501 cctcaaggtc aatatgaagc agcgcaagtt ttgcaattaa gtccctttca aacggtgacg
     1561 aaaatcgtca tcccacaagt gacaaagatt gttttacctt ctgtagggaa tgaagttatc
     1621 aacttggtca aagattcttc cttgatctat atattaggtt taggtgacgt aatgcgtgct
     1681 gggaaaatcg cgatggaacg cgacgcgact ttactgcctt tgctaggcgt ggcaatcatc
     1741 tatcttacat tgactggtat tctaacaatc gtaatgaaac aagttgaagg aaaattaaat
     1801 tattaccgct aactgatagg aggaagaaat atgattgaat tgaagaaagt gaacaaagca
     1861 tttggtgaaa aaaaaataat caaagatttg aatctaatca ttccagatgg gaaaacatta
     1921 gctatcgtag gtccttccgg tggcggtaaa accacgcttt tacgtatctt ggctggctta
     1981 gaaagttctg acagcggaga attttattta gacggtgtac cgttcaaccc attcgagctt
     2041 aaaatgaaag aacaagttgt aggagtcgta ttccaagact tccagctatt tccacatctg
     2101 aatatttttg aaaacattac cttagcacca aaaatggtat tgaaacagac aaaagaagaa
     2161 tacaccaaaa aagcagaaga attagcggct tcattaggga ttagtgattt attgaatatc
     2221 tatccttatc agctttctgg cggacaaaaa cagcgggtag cgattgccag agcattggct
     2281 atgaatccac gggtattggc ttatgatgaa ccaacaagtg ctttagatcc tgaattacgc
     2341 cagcaagtcg agcgtttgat cctatcattg aaagaagatg gggtaaccca aattattgtc
     2401 acccatgatt tagtatttgc agaaaatgtc ggtgatcaat tactaaaagt ttccccaatt
     2461 cattaagaaa ggagaagata gagatgaaaa aaaccagttt tctattcgct ttgatcgcag
     2521 gtcttcttct ttttgcaggc tgttcgaaca aaaaaacttc agcagataat tgggcgacta
     2581 tcaacgaaga gaaaaaaatc gttataggtt tagacgatac atttgttcct atgggattta
     2641 gagataaaga cgggaagctc acaggatttg atattgattt agccaaagca gtctttgaac
     2701 aatatgggat tacggtagac tttcagccca ttgactggtc aatgaaggaa tttgaactga
     2761 acaatggaac gatcgatttg atatggaacg gctattccaa aacgccggca agagaaaaga
     2821 aagtgcaatt tacaaaacct tatatggaaa acgatcaagt gctgatcaca cctaaagaat
     2881 caggaatcac aaactttgga caaatgaata ataagagatt aggtgcgcaa aatgggtcat
     2941 ctggttatga tgtatttacg aaacaaccag aagtattaaa agagatagtc aaaaaccaag
     3001 atgctgtttt gtatgatagt tttaatgaag cattgatcga tctgaaaagc agacgggtcg
     3061 atggtctgct gatggataaa gtatacgcag actattattt gaaacaaaga aacgaactag
     3121 aagacttcaa cataacaaaa ggttcttatg agagtgagga ttttgcagtt ggtgcacgca
     3181 agtcagatag tacattagtg aaaaaaatca atgaagccct agatcaacta gagaaaacag
     3241 gtgaattcca aaaaatatcc gataaatggt ttggcgaaga tgttacgccg agaaattaac
     3301 aaatagggga ctgtgacaaa agacgtgtca cagtcccttt ttccgaataa acagtgttct
     3361 cctgccaact ccttgtgact cggag
//
LOCUS       DBNICI010000208         3355 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.212, whole genome shotgun sequence.
ACCESSION   DBNICI010000208 DBNICI010000000
VERSION     DBNICI010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.212"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..190
                     /locus_tag="AC1ZCC_002672"
     CDS             <1..190
                     /locus_tag="AC1ZCC_002672"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630882.1"
                     /translation="DLEKLTKQRDMKNFNNCVVLIIITELTLFTNIYYIHEAQSSLIE
                     DWAIFMAKEGKEMFQNKG"
     gene            203..478
                     /locus_tag="AC1ZCC_002673"
                     /pseudo
     CDS             203..478
                     /locus_tag="AC1ZCC_002673"
                     /inference="COORDINATES: protein motif:HMM:NF017894.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
     gene            505..804
                     /locus_tag="AC1ZCC_002674"
     CDS             505..804
                     /locus_tag="AC1ZCC_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HIQ1630883.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            978..1529
                     /locus_tag="AC1ZCC_002675"
     CDS             978..1529
                     /locus_tag="AC1ZCC_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HIQ1630884.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1675..1968
                     /locus_tag="AC1ZCC_002676"
     CDS             1675..1968
                     /locus_tag="AC1ZCC_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HIQ1630885.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            1961..2257
                     /locus_tag="AC1ZCC_002677"
     CDS             1961..2257
                     /locus_tag="AC1ZCC_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630886.1"
                     /translation="MAKLIVLGGLIYGGYWLYQYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            2332..3330
                     /locus_tag="AC1ZCC_002678"
     CDS             2332..3330
                     /locus_tag="AC1ZCC_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HIQ1630887.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
BASE COUNT         1263 a          459 c          664 g          969 t
ORIGIN      
        1 agatttagaa aaacttacta aacaaagaga tatgaaaaac tttaataatt gtgtagtttt
       61 gataattata actgaattaa ctttatttac aaatatatat tatatccatg aagcccaatc
      121 ttctttaata gaagattggg ctatttttat ggcaaaggag ggaaaagaaa tgtttcaaaa
      181 taaaggataa cgttatgtca caaagggagt aatggacagt ttgccggtgg aattacaagc
      241 tttatgttgg aatctaatcg atcaaaacgt tcaaaagcaa tttcctttag actatcttca
      301 aatttttgaa ttttctacgg aaaaaggaaa tcaaaagttg gttcatcgtc aagaagaacc
      361 ggaagaaaga aaagagtatc tgatttcacc taagcttcgt ttgaaatcag tgtctcaaaa
      421 aatatgggcc attgattcgg gtgagtatca aacgatgtta ttaccagaag aatactagta
      481 tctaaaagaa aggaacgaaa ggacttgtta gaaacggcta tcgaagtact ggaaaagtgt
      541 gcacagctgg tcactgcttc tgaagagtgg ggctatgaat ccgttacgat ggaaaaagag
      601 gaaattgaaa tgggtatgct tcctgaagat gttcatctac cacgtttagt gatgacacat
      661 ctttatattt attgcgcacc agaagatgga aaagactatg tagtttattt cattacagat
      721 attaccagcc agcgagaatt tgttcgtggg ttactggtag aaggacgatt ggtttggagt
      781 cagattggag gaacggatga ataagatcga ttatttgaga atgattgcga aatgtttaga
      841 agatatcctt accgcgattc atcaaattct gaagaaaaaa ggagaaagaa tatgaaatat
      901 tttcaactag tattgacact tataacggca atggtaattg aagtgcttga acaaaaaata
      961 gcataggaga aaaagagatg attgaaaaag aattagtgac cacacaaaca gaagtttggc
     1021 gagatcctca tcatacccat cgctatgttt tcaaacgaca atgggcgatc aaaggaaaag
     1081 aagcggaaga taaattagca gtagtcatta ctattcgtcc aacagataca gaaccattta
     1141 cgaatgattt gagtatgtta ttaatcgaaa agaatatccg acaattagga tttactggtt
     1201 ttattgctgt gaatcttttt tcgtacacaa aagcgaaaag tcctcgggta tttcttcgag
     1261 gaaatgatga acaatctttg gaaatattaa cgactgtttt tacagaaaag aaaatcaata
     1321 ccattatttt tgcttgtggt tcgattacag cgacgagtca agtggcttat gaacaagcaa
     1381 agactattta cgatcaattg agtgcgaaac aaaagaagat gaccaaagta ttgataaatg
     1441 cagaaggtaa gttatcccac ccactaagta ttcatgttcg taatgaatgg caattggcag
     1501 atagtcaatt attatttcaa gaaaaataac attttatttt ctcatgttac ttataagtag
     1561 ttttacatgc tttttgactt taaaaataca tgtgatgata catgttatat gaattatcaa
     1621 tgtacccagt aatatgctat tttcattatg taaaaaataa cagggggtgt gtagatggag
     1681 attgatgaaa cttgtttgag taactcttat aaaagtatga atgacttttt tgaggaaatg
     1741 gaactggatt cagaattgct ctatcaattt tatcttgctt ttcgagggca acagatttct
     1801 tttccaatga aaatgtacga tcgtgagtta gttagaaaaa gaatcgaaaa catgatagaa
     1861 caggaaaaaa ctgtagatat ccgtcaattg acagaagttt atggtttctc tgcaagatgg
     1921 attcaaaaag taattaaaca aaaagaaagg agacgagtac atggctaagc tgattgtatt
     1981 aggtggttta atttacggtg gatattggtt atatcaatat atgcttgcgc atccgaatat
     2041 agataaagga aaagtaatta agacagttgg ggtaatcata gttggactca tagctctaac
     2101 taagaaaaat aatactaata gcccaaaaat aatgagaact tatcgctgta gaggatgtgg
     2161 aagggaaatt tctactagtt ttaatccatc tgagagtcct caaggaagag accgctgcca
     2221 tacgggctat cgacattatt gggaaagaat cgattaaaag attaccattt agatcaatta
     2281 attgtctgat agaataagta cgtgagggag aaattgaaag ggggaaatat aatgaaaaaa
     2341 tggctgttat tactaatagg aatgggaatt ttattcactg gttgtacatc tgagaatacg
     2401 aggactaaag cgactataga aactgataca acaattgaga ctaaaaaaag taaaacgcaa
     2461 tcaactgaac aaactacaac agaaatgaag caatctgtgg aaacaagtgc aagttcaatc
     2521 actgaatctt cttctgaaga ggttaagaat actttgtggg atacgaataa agcttccaaa
     2581 ttagaaactt ttgtcacaca atggggaaag acattaggtc aagaatataa aagctacaat
     2641 cttcagaata atgtaagtct gtatggcact cctttaccac aagccgtgat taatggcgat
     2701 tggaaaatgg caatcaatga agcaccagtt accgtacaat ggtcagaaga tggaactggt
     2761 catgcagatt tcaatttagt agcagtttat tctgatgtag aaactggaga atatttaggg
     2821 cagcatgttt actttttcgg ttttcaaaat ggacaaccta aagtttattt aacgcaacaa
     2881 aatcaaggta atgaaaataa ttatctgtac tttaacgaaa ctcaaaatca acagttaaaa
     2941 caaggtttca gtgatattgt gaatggacaa actcctcaaa caccagtagt agaagaatct
     3001 actcaacaag caacaactac aacgacacaa tctacaagta gttcaggaat ttcaagttgg
     3061 gaagctgcta aagaatctgt gctaaataat aaagctttat ggttgtatga ttctcctgat
     3121 aaaaatgtat caaatactaa tttaattagc acaaatgaat atatgaaaga tctaatgcaa
     3181 gatgaaaatg gaagctacta ttcaattaat attgtaacgg atgatgatgg aaacacgacc
     3241 atggctggtg gaaaacaatt tcgtgtttat acagatggaa gaatttctca acgtattgga
     3301 atgactaaca attttcaaat gattaaataa ttgaaaatat tatttatcaa agaaa
//
LOCUS       DBNICI010000209         3231 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.213, whole genome shotgun sequence.
ACCESSION   DBNICI010000209 DBNICI010000000
VERSION     DBNICI010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.213"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..1438
                     /locus_tag="AC1ZCC_002679"
     CDS             <1..1438
                     /locus_tag="AC1ZCC_002679"
                     /inference="COORDINATES: protein motif:HMM:NF012307.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HIQ1630888.1"
                     /translation="SKKNQPKDIKDIVDTRMYKERDDLNYTLIINKDGNYSWRPLVLL
                     HPILYVDLVNCLTKEDHWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILN
                     WWEYFEQKSLAQNIKYQYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLG
                     DKIDKKISGMQHNQTNGIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRY
                     RDDYRIFSNNRNDLEQIMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEI
                     KASIRMNATNLDGDREIRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKI
                     TSKPNDLLPLIGILVDIMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALK
                     PNTEFMMIWFQRLTTIFGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEG
                     TIKDEEKYIKMSKKIYITEFDDFNDYPF"
     gene            1776..>3231
                     /locus_tag="AC1ZCC_002680"
     CDS             1776..>3231
                     /locus_tag="AC1ZCC_002680"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HIQ1630889.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMMARMNMILH
                     GVPIANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVL
                     APKSKADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGL
                     PANIFFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKT
                     RTIKDKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
BASE COUNT         1185 a          507 c          588 g          951 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaaagcactt tctgagttta ataaagaacg cattattaaa atcacttcaa agccgaacga
     1021 tcttttacca cttattggta tcctggtaga tatcatgata aataacccgg gttctttgcc
     1081 gcagtgtgtc ttaattatag ggcagttatt aaatttcgaa aatgagagta aggctagaga
     1141 aatagttgaa atgattataa acaaatatgc tttaaagcca aatactgaat ttatgatgat
     1201 atggtttcaa agattgacca ccatttttgg agaaatgttt gatagtgttt tatcatccga
     1261 atttgtatct aaaatcaaag atcctgtctc taatcagatg ttttctgtag actggctaaa
     1321 tgaagatgct aagaaatctt tttatgaagg gaccattaaa gacgaagaga aatatattaa
     1381 aatgagtaag aaaatatata ttactgaatt tgatgatttt aatgattatc ctttttaagt
     1441 gaaaatttag aatgaatata aaaaacatta caatctaatt tttttaaaaa aatgttatat
     1501 cggaagtaat ctcttgattt ctatactata agataagaaa accactattt aacaaggtta
     1561 ttcgaacctt attttaagtt acttattatt taatgtatcg aaaaaggtga aataggtttg
     1621 ttataataag aattaagtaa gacatgaatt gtctaagcaa atgtagctta tccatttcat
     1681 taaaatacat atggtgaaaa aacaagttca aatagtaact aattttttaa aaaactgatt
     1741 tataaaaaat acaacacgaa tgaggaaaat aaccaatgac agaacaaatc gaaaaaaatt
     1801 caaaaagctt atatcaggca ctgtggaatt ccgcagatat cctgcgttca aaaatggacg
     1861 ctaatgaata caagagctat ttactaggct tggtctttta taaatactta tccgacaaca
     1921 tgctgcggta tgtctccgtt ctattggagg aagaaacgga cgacctaaat aaagcacaag
     1981 acctttacgt tgaagcattt aaagatgcag atataaagga tgatctcttg tatgaactaa
     2041 aagatgagtt ttcatatacc atcgcacccg cattgacctt taccgcgcaa gtggcagcca
     2101 ttcatgatgg atcttttcaa ttagaagacc tggtacaagg tttccgtgac attgaacaat
     2161 ccagcgaaaa ctttgagaac ttatttgaag acattgatct gtattctaaa aaactggggt
     2221 caaccccaca aaaacaaaat aaaaccatcg cagatgttat gaaagaatta gaagggttaa
     2281 acatggcggg gcatgctggc gatgtcttag gcgatgccta tgagtactta attggccaat
     2341 tcgcatctga atccggtaaa aaagccggtg aattctatac cccacaaccg gtcgcaaaat
     2401 tgatgacaca aattgtttta caaggaaaag aagataaaaa aggcttttct gtttacgacg
     2461 cgaccatggg ttcgggctcg ttacttttga acgctaaaaa atattcccat caaccgggaa
     2521 ccatttctta tttcggacaa gagttgaata cgtctaccta taatttggcg cgtatgatgg
     2581 cgcgtatgaa catgattttg catggggttc ccattgccaa ccaacacttg cataatgcgg
     2641 ataccttgga tcaagactgg ccaacagaag agccaacgaa cttcgatggg gtgctcatga
     2701 acccaccgta ttctgcaaaa tggtcggctg ataaaggctt cttagatgac ccacgcttct
     2761 ctgcctatgg cgtattagcc cctaaatcaa aagcggactt tgctttctta ttacatggct
     2821 actatcattt gaaagatacg ggcgtcatgg ccattgtttt gccacatggg gtgctcttta
     2881 gagggggcgc agaagggaag atacgcaaag ccttgttaga aaatggtgcc attgacaccg
     2941 tcattggctt acctgctaac atcttcttta atacgtccat tccaacgacc gtaattatct
     3001 tgaagaaaga ccgtgcgaaa aaagatgtgc tctttattga tgcgtcacaa gattttgaaa
     3061 aaataaaaac gcaaaatacg ctacgagatt accatattga cgctatttta gaggcctata
     3121 aaacccgtac aattaaagac aagtatgctt atgtcgcaga atttgatgaa attgtggaga
     3181 atgattacaa cttaaacatt ccccgttacg tggatacctt tgaagaagaa g
//
LOCUS       DBNICI010000210         3210 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.214, whole genome shotgun sequence.
ACCESSION   DBNICI010000210 DBNICI010000000
VERSION     DBNICI010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.214"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            34..666
                     /locus_tag="AC1ZCC_002681"
     CDS             34..666
                     /locus_tag="AC1ZCC_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HIQ1630890.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLTGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            1254..2693
                     /locus_tag="AC1ZCC_002682"
     CDS             1254..2693
                     /locus_tag="AC1ZCC_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIQ1630891.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2988..3206
                     /locus_tag="AC1ZCC_002683"
                     /pseudo
     CDS             2988..3206
                     /locus_tag="AC1ZCC_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
BASE COUNT          979 a          520 c          716 g          995 t
ORIGIN      
        1 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
       61 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
      121 aatgaaacga ttgtatatcg atcaaactta ctaacaggta gaaccaaaag ttgtggatgt
      181 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
      241 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
      301 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
      361 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
      421 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
      481 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
      541 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
      601 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
      661 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
      721 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
      781 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
      841 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
      901 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
      961 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     1021 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     1081 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     1141 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     1201 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     1261 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     1321 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     1381 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     1441 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     1501 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     1561 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     1621 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     1681 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     1741 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     1801 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     1861 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     1921 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     1981 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     2041 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     2101 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     2161 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     2221 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     2281 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     2341 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     2401 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     2461 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     2521 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     2581 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     2641 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     2701 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     2761 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     2821 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     2881 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     2941 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     3001 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     3061 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     3121 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     3181 gccttagaaa acatcaagaa ccttaggaac
//
LOCUS       DBNICI010000211         3102 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.215, whole genome shotgun sequence.
ACCESSION   DBNICI010000211 DBNICI010000000
VERSION     DBNICI010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.215"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            483..569
                     /locus_tag="AC1ZCC_002684"
     CDS             483..569
                     /locus_tag="AC1ZCC_002684"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HIQ1630892.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(871..1095)
                     /locus_tag="AC1ZCC_002685"
     CDS             complement(871..1095)
                     /locus_tag="AC1ZCC_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630893.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRS
                     LEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1461..1745
                     /locus_tag="AC1ZCC_002686"
     CDS             1461..1745
                     /locus_tag="AC1ZCC_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630894.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1748..2164
                     /locus_tag="AC1ZCC_002687"
     CDS             1748..2164
                     /locus_tag="AC1ZCC_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630895.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2301..3020)
                     /locus_tag="AC1ZCC_002688"
     CDS             complement(2301..3020)
                     /locus_tag="AC1ZCC_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HIQ1630896.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
BASE COUNT         1026 a          587 c          482 g         1007 t
ORIGIN      
        1 cttctgtatc cgttgggaga gtaaggctca actgccccct aaaaagggtt ctaaaaaatc
       61 tttaaaaaat aaataaaata aacgcgcgcg cgcgagcaaa atttcccgcc aaaaaatcac
      121 ccacttttta aaataaacag gttcgcgcat gatattaaac tgccaatcta gctccctatg
      181 gtcgctgtaa gaaggagtga tcgctacgct ctcacatttt acttgcagca gtcgccttta
      241 ggctccttcg ccaaaaataa ttcattaggg actcctttgt ggcgtcctga gagctcgtct
      301 aagccgttct aagacaagat tcgataaaaa ctatgtcctt cgttctaaag cgccttacag
      361 gcggtcaggg caagccctaa caaccccaag gcaataaaaa acttaggctt atttttgctt
      421 ttttattgta taattgattg tcgcctatcc gacagtcttg cgactggaag gaggtgtgtt
      481 tcgtgttcac taactttatt tgctcccttc ttgttgggct tttaagcgct ttgttcagtt
      541 actggttgaa taaccgaaac aagaagtaaa acttaattaa ggcgactcag cccacaaaaa
      601 aagccccaga gaatccgacc tctctggggc tttcgtgtgt ttcgtgttca cttatttgca
      661 atcaaattat aacagaaata aatatttatt tcaaaaatat cctacaaaag tctaaaatcc
      721 gtaattattt cttatatgca acatttacaa ataaagtcgc tctgagagct cgtatttttc
      781 gatttaagac gttttattta gtttttggtg tatttcattg gtccaagcta aaaaactctt
      841 agaagcgatt ttagagccat ttaaacccac ttaattatat tctccatgaa attcgctcca
      901 tgcgtcaaaa gctgcgtctg aaattccgcc gtcttcgttc actagttgca ttaatttttc
      961 caacgatcgt tttctttcgg gtgtcatatt tttatattcc aacaattttt cgtctgttaa
     1021 atcgaacttg attgcttgaa ttgcttgctc taatttttct aagttttgaa aatattcgta
     1081 aggaaatttt tccatttttt ctttctccag tctcacaatt tttctagaaa taaaatcact
     1141 tctttgccgt ctatactctc aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc
     1201 gtgcattttt aaatttggtt atcgattaca cgtgcagcac tcccccccga attttaaaga
     1261 gcataaggaa acgtgtaccc actttctaaa aaaagtgggt agataggctg tgcctatcac
     1321 gtttctcaga aaaaaatttg atatcacttt ttcgccatcg tgggtagctt atgtatagca
     1381 ttttggcaaa aaacagctat acaaatatag tatttgtatg gtatacataa attatacata
     1441 attgcaaaag gagatttttt atggacattt tagttcggaa tattgatcca aagtttgttg
     1501 caacaataga caaacgttgc caagaactca cgaataaaac aggagaaaaa tggagtcgaa
     1561 ataaatatct aaaagtttta atcgaaagtg attttgaccg tcaacttacg acttacaaaa
     1621 aagaccaatt cgatttattg ctcgataaat ttattgctat ccaaacagaa aatactcaaa
     1681 tattaagcga gtatgtctac acaaataatc aaatcattga aatgcttatc ggaggaatga
     1741 tataaccatg agaaaaactt ctgtccaaaa acatgttact ttgtacccta gtcatgttga
     1801 cgttttagat tccatcatcg aaaaaaatcc agatatcaat aactactcgg aagccattcg
     1861 ctttttatgt atgagttatg aacccgatac caacacgacc actaccagca atcaggcaaa
     1921 actaaatgct atgggaaaag aaatttccat catgactgaa atcatcgcag aatttgctga
     1981 tttatatcta aaagatacag gcgttcttat tggcacaaat tctaaagtat atcaagaagt
     2041 gaaagaaaaa atcgaagcta aactaaatcc atcaaatcga aatcgaccaa agaatcatca
     2101 aaaagaaacc agtcaaacag tcacttctag aaatttcgac ccaacaaatt ttctaaaacg
     2161 ataaaaaaca gataggagtt attcctatct gttttttatt tagaagcccc atacaatatg
     2221 ctagaatgta ctagcaatct tagtcggttg taagggactt gtctacttgc ttaatgtaag
     2281 gggggcgagt tcttttttat ttattcttct aaccagttac gcagagaaat ctttggcaaa
     2341 tctgacgtta ggcttgaaaa agtaaattct aatttggcga ttttattgcc cttttttgct
     2401 tttatttttg tgactttaag atcattgaag tattttttta gttctcgtaa tgcaggattt
     2461 aatacttttt gatcaatgtt acccatttga tagtattctg ggatatccat taagctccta
     2521 aactcttcaa tacgaactac ataataacca gtagaacggt actgcataag cagtcggaat
     2581 agagtttttg catacgtaga acgcaacgac gtgaaagcag ataattcaaa tctactaaat
     2641 tcagctgtta aaccattaat aatatattct aggtccggat taacactgac ttcaacaaac
     2701 ttttttgttt tattgatttc aaaaccagta aacaaaacaa agtagcgaat agaatcctca
     2761 tcttcagtac ggtaagtaag attaagcata tctttataga gcttttctaa atcaccgaca
     2821 aaggcttttg tagcagtcat cttataatca cttaactctt ttaattcctc gaaagaaaat
     2881 ctaacttttt tcaaaccacg atctttcata cgagcgcata atgcaaaaaa taaatccatc
     2941 tgcttagcat tgaaattttt cagcggtact aaattcaatt cattttgata tttgactgtt
     3001 aattccgttt tttcatccat tcctttcttc cccccttaca aataaataat aacctttaac
     3061 aaccaactcg taaataggtt ggttgttatt atacaaaagg at
//
LOCUS       DBNICI010000212         3101 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.216, whole genome shotgun sequence.
ACCESSION   DBNICI010000212 DBNICI010000000
VERSION     DBNICI010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.216"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            199..1032
                     /locus_tag="AC1ZCC_002689"
     CDS             199..1032
                     /locus_tag="AC1ZCC_002689"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIQ1630897.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(1134..2834)
                     /locus_tag="AC1ZCC_002690"
     CDS             complement(1134..2834)
                     /locus_tag="AC1ZCC_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630898.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
BASE COUNT         1097 a          512 c          618 g          874 t
ORIGIN      
        1 gtttgaatac tgtttagtgg agaggtcgtg aaaaagctgc cctgcaccaa gtgataaaaa
       61 cagccaaaca aaaaatagct cctggtccat tgagcttatt actgaggagt tagcaggaga
      121 acactatttg ctggagaggt tgtgacaaaa attttgtcac aacccttttg tttcgaaatg
      181 aagacaagga gaaaaccaat gaaattcatg acactcaaca cgcatagctg gcttgagcca
      241 gaaccagaga aaaaattgca agaattagca gataagatcc tgttagaaga ttatgaagtg
      301 attgctttac aagaaatcaa ccaattactg gaatctgaag aagtagaacc tgcgaagcta
      361 atgaagttct gtccggtaaa gaatcaagta cctatccgta aagataattt tgcctaccgt
      421 ttagttcagc ttttaaagga acacggaaaa gagtattttt ggagttggga aatgagccac
      481 atcggttatg ataaatatga agaaggaaat gcactgctta cgaaaaaaac attggaaagt
      541 caggtcctta ccgtctccca atttcaaaag aaagaagatt atcagacacg caagatcttg
      601 attgggaaaa caaagataga tgatcaggac atttttgtct cgagctgcca cttttcttgg
      661 tggacagata aaaaaagtgg cttttatttt gagtggaaaa acttggaaaa ctactttttg
      721 gaaactcgcg taccactttt tttcctaggt gatttcaata atccagtaga ctctcaaggt
      781 tattatactg taagagaaag ttgccttctt cttcaagatt cgtatgttgt cgcgaatgaa
      841 aaaggaaaag cagctactgt ggaaaagaaa atagatggtt gggaacaaaa tacagaaaaa
      901 ttgcgtatag attttatttt cgtccctgaa ggaatgcagg ttaaaaaata tcaacggatt
      961 tttgatggga tcgatagtcc aatcatcagt gatcactacg gggtggaaat cgaattagat
     1021 gtgaacgaat aaaaaatata acatataaca taaaaaaact gattaaaata aaactctcca
     1081 aacgattata ccgtttggag agttttttga tcggatgtat ttatcccttt tgcctaagca
     1141 cgtaaaagct tgtatctatg ataaataaat aaacatagca aggtaaacca gccaatcaaa
     1201 gtaagagaaa gaatgaaata gaaatagatt ggcgtttgat aatgaagcgt gagctcgttg
     1261 attccttttt gctgcttcac gattggggtc ccgatatctg ttacttcata atcagtcaat
     1321 gtttttccat tttgctgtag cacagtccga tcgtagacga taacagggat atgaacagtt
     1381 ttgtccccag tgtttttcca cttgattgtc aaaagaccgc ctggatgtgt ttttataaaa
     1441 gttgtttcgt tttctagtat ttcttcgtta taccggtcgt atttattgtc ttttgtctcc
     1501 ttatagattg gcagatagtc aggtgtggtt ttttggaaaa cgaacaagct ggtagacata
     1561 tctttgctga agaatgtttt ccttgcttct tccggcgtat cgaatagata agtatgtcga
     1621 ctgacaaatg tttcattttc ccacttttcc agatgctggt ataagttctg gatcgtctgt
     1681 gctgaaaaaa taagaatgcc aaatatactt aaaagacgga accacttttg ttcagaccac
     1741 tggttcagtg aaagggcaag gtaaagtaga agcaatagcg taaatggcac gaaaaatcga
     1801 aatggaaact ggatcaaact aataaaaggc acattttttt cgatcaatgt cgtccatgga
     1861 atcaagctag tagaaagaaa aaagaaaaca agacttgtgt acaacacgat tttttggaat
     1921 aaggtgttct tttgttgaaa gaaaataaaa agcaaaaaac ctgataatat caatatagga
     1981 aaggccttag ggtaatggag ccaataactg ccatccagat ctatcgcctt ttgactcatt
     2041 tctctgttaa caaagggagg aagaatctta ttcccactgt aaacaatagc taatcctgcc
     2101 caaatatttg ctgtcagaac aaaataaagc aagaccgact taatcagttg aagcagtgct
     2161 tttccttttt cagtctggtt aaaaaagtaa taaagataaa aagggatata catcaaagct
     2221 aacatgatcg aagacagtaa atggatctgt gtcataagtc ccactgagac agcgactttg
     2281 ataacaggga actttttatg aaggacgaaa tcagcaatag ggatcataca taaaggataa
     2341 aaacaagtcc cccagctagt gaatccttgg ttgatagacc agtattgaat agaatacgta
     2401 ctcatgaata aaacggctac tggtacactt acccaatttc gaacattgac atatctcagt
     2461 aaaatcagca tagaaaatcc tgaaataaga taaagaatga aatttgaaag aatctgataa
     2521 tgaaaccagc tacctgcaag caacacgatc aatccttgta aataagcgaa aaatgggccg
     2581 taaacggcat tcacgatcct gccactttgc tggaatccat aaagggaagg aaaataatgg
     2641 aaatccccat atttaatttg ttgcgcggca tcatagaacc gattgtaatg gaatggggca
     2701 tcagcaccca aaattagttt atgtgtgaat aaatgaggca tgagaaatgc caaagcaatg
     2761 aaaaaaattg ctaaaaatgg gccatatttt ttcaattgtc gaactccttt gcaaacttta
     2821 gataaattca tcatatatcc tatgcgagag gtaggcaaga aattagaaaa ttcatgaaaa
     2881 tatttatgaa ttttacaaga aaaacataag tgaaagttct aaggagagag gatgcgacat
     2941 aagttttgtt gcatctcctt gttttgaaac agaatttttc ttaatactcg aaacaaagta
     3001 gctttttcat gacctctcgg taaacggtgt tcaatcagct gcttctcggt aataagctca
     3061 ctagcctaga aaatatgaga agctattttc tgactagcgc t
//
LOCUS       DBNICI010000213         3023 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.217, whole genome shotgun sequence.
ACCESSION   DBNICI010000213 DBNICI010000000
VERSION     DBNICI010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.217"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(287..649)
                     /locus_tag="AC1ZCC_002691"
     CDS             complement(287..649)
                     /locus_tag="AC1ZCC_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630899.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="AC1ZCC_002692"
     CDS             complement(723..1103)
                     /locus_tag="AC1ZCC_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HIQ1630900.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="AC1ZCC_002693"
     CDS             1185..2360
                     /locus_tag="AC1ZCC_002693"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HIQ1630901.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="AC1ZCC_002694"
     CDS             complement(2437..3021)
                     /locus_tag="AC1ZCC_002694"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIQ1630902.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          942 a          578 c          498 g         1005 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tat
//
LOCUS       DBNICI010000214         2998 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.218, whole genome shotgun sequence.
ACCESSION   DBNICI010000214 DBNICI010000000
VERSION     DBNICI010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.218"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..285
                     /locus_tag="AC1ZCC_002695"
     CDS             <1..285
                     /locus_tag="AC1ZCC_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630903.1"
                     /translation="DEKIVREIDRRCDELSNRTGNKWSRNDYLKLLVENDFDRPLMEY
                     KQDKFDQLLNQLIDKFSFVQSHNTKVLEEYICQNNQIIELLIEQSRGGEV"
     gene            287..709
                     /locus_tag="AC1ZCC_002696"
     CDS             287..709
                     /locus_tag="AC1ZCC_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630904.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     SEVEKKVNSNQAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            729..2318
                     /gene="mobP2"
                     /locus_tag="AC1ZCC_002697"
     CDS             729..2318
                     /gene="mobP2"
                     /locus_tag="AC1ZCC_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HIQ1630905.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTGKEFSETSNEAQSLMKHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKVLTHSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQTFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLTEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            2330..2665
                     /locus_tag="AC1ZCC_002698"
     CDS             2330..2665
                     /locus_tag="AC1ZCC_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630906.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYSATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            2811..>2998
                     /locus_tag="AC1ZCC_002699"
     CDS             2811..>2998
                     /locus_tag="AC1ZCC_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630907.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         1195 a          438 c          608 g          757 t
ORIGIN      
        1 gatgagaaaa tcgtaagaga aattgatcga agatgtgacg aactaagtaa ccgaacgggt
       61 aataaatgga gtcgtaacga ttatttaaag ctactcgttg aaaatgattt tgatcgtcct
      121 ttgatggaat ataaacaaga taaatttgat caattattaa atcagttaat agataaattt
      181 tcttttgttc aaagtcataa tacaaaagta ttagaagaat atatttgtca gaataatcaa
      241 attatcgaac ttttaattga acaaagtaga ggaggagaag tttaacatgg ccaaagttat
      301 agacaagcat atttattttt atgaaacaca tatagatgtt ctagatgaaa ttattgcaac
      361 tcatgcagag gttaaaaact atagtgaagc cgttcggttt ctatgtatga attatgattc
      421 agaagtggag aaaaaagtaa attccaatca agcaaagttg aatgcaatgg gaaaagaaat
      481 ttctatttta accgaaatgg tttcagagtt tgcggatctc catttgaaag atacagggat
      541 tttaattggt acaaattcaa ctgtttacca agaagcaaaa gagcgtgtgg aaggcaagat
      601 aaaagcaaat caaacgaaga aatattctcc taagataaag aaaacaataa gcgaatcagt
      661 gactacaaaa agtgaagtat ttgatccaac aaggttctta aaacgataaa aattagaagg
      721 agaatgtgat gagtaaatca cctgcagtca ttctcacttg tcaatttacc ttgcccaata
      781 aaaaatcttt ttcaacatac attgattata tgacaagaga aaaagcgctg gaagaaaagg
      841 aacgacgtac gcccgaggaa gaacaggaac tgcgtctaat caagaatgct ttagaggatt
      901 tttatattcc tgaaggagaa actttttcgg agagtaagac gggtaaagaa ttcagcgaaa
      961 cgtcgaatga agcgcaatcg ttgatgaaac atggaatcaa ttttgacaag ttagaagaac
     1021 aagattttac caaatatctt tcttatatga cacgtcacta tgcgttagaa caaaagaaag
     1081 tactgacgca tagtgaacaa aaagaatacc aacgcttgaa ccaagcaata aaaaaatatc
     1141 aaacagagcc acacaaaaaa gcttcaggaa atcttcctgg agttttttct atggcttcag
     1201 acgaagtgaa aggaagtgat ctgaaggaga tcaagcaaac cttccaaaga ggacaacaaa
     1261 aaggctcaat catttatcaa gatgttgtct ctcatgatca ttcgtatctt gaaaaaatag
     1321 gattgtacga tcccaaaaca gatactttag atgaagaagg tttaaaagca gctggaagaa
     1381 aaatgatgga gacattgttc gagaaagagc aactgaatga aacaggctac tggatggcaa
     1441 cgattcaccg aaataccaaa catatccata ttcattttgc agttgttgaa aaaaaaaata
     1501 cgcgtaaacc atacactgag attgaaaatg gtgtgagtta tatcgtaccg aaagggaaga
     1561 gaaggcaagc aacattagac gaaatgaaga ctaattatgt acatgagtta gaacgttttt
     1621 cttatgaaaa aaatcgaatg ggtatggaga aacgaaattt attgacacgg aaaagtgatt
     1681 tgcgtaacac cttaaccaaa gtagtgaaag agccagtacg ttatgatcga cacgcaatga
     1741 gcttattaac tgaagtatat aaaaacttac ctaaaaagcg ttcggaatgg cattacgggg
     1801 atgaaaatcg aaccaaactt tcaccataca caagagagaa actcgatgag ttaacccaat
     1861 atgtgttaac aaaagaacct ggatatcaag aatatataca gatttcaaaa gcattgaaag
     1921 aagaagcaaa aagtctttat ggggaaagca agagagaatc aaaggatgca gaaaaaaatg
     1981 ctctctttga tttaaaaaag cgcacaggaa atgccattct aacagaactg aagaaacagg
     2041 atagtcaaat aaaaccttta atagaaagga tggatcagat gcaatctatt tacaatgaac
     2101 gatcctttcg aacacttaaa cagttgccaa cgaatgaata caaatatagt tacgaagaat
     2161 tcatgaagcg caggaaacaa ttccaacaaa gagttgtcac gcaacggagt ttgcgtcaat
     2221 taaatcgaac gattaatgcc catgaagaaa aataccacgc aagtaaagca tacgaagagc
     2281 aacaacgaag aattcaaatg gaacaagaaa gacagtagga ggaatagcaa tgaaacaata
     2341 tttagatcaa tggaaagtta tagaaggatc attaagagaa gaacgaatcg aacaacttcc
     2401 cgattgttta gaaaaagagc atttgtttca gataagagaa atgttgagaa acgaacagtt
     2461 tgatccaaat caatttttag tcgttgagta ctcagctact ggtgtgtact gttgtaatca
     2521 tgtgaagggt gaaaaatatt ttatcattca agaatatgag ggcaagttag cgccttatta
     2581 tactacttgg gaaatgaatg aagaggggat caataacttc ccgtgtaaaa gcattgaaga
     2641 gtctatttca ctcacagaat gttagttgtt aagtgaataa aagaagtaaa agagtcctga
     2701 acgtaattgt ccaggactct ttttttcaat gaattgtttt ccgccaattc aatgagtggt
     2761 gcgatttgcg actacattat atcaaaagaa agagaaggga ggaagaagaa atggcttatt
     2821 caacgattgg agttgttgtt gaaaaaagca gagacaatct cgtttttgtg acagaaattc
     2881 aaacgggcag agcttttgtc gtcacagaca aagcggcaaa agcgtatcaa agtggcgata
     2941 ttttaacgtt aaatctgact acgaagacgt tcgtcgatgc ggcagaagat tatccatt
//
LOCUS       DBNICI010000215         2966 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.219, whole genome shotgun sequence.
ACCESSION   DBNICI010000215 DBNICI010000000
VERSION     DBNICI010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.219"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            240..551
                     /locus_tag="AC1ZCC_002700"
     CDS             240..551
                     /locus_tag="AC1ZCC_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HIQ1630908.1"
                     /translation="MDELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     K"
     gene            548..766
                     /locus_tag="AC1ZCC_002701"
     CDS             548..766
                     /locus_tag="AC1ZCC_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HIQ1630909.1"
                     /translation="MISLTIFWCVIGGLLAMLGAYLIYESAKGKKYTAVGIILFVVGI
                     LVAIGAEINLYLNGTTEELMWFLLWTRR"
     gene            779..1060
                     /locus_tag="AC1ZCC_002702"
     CDS             779..1060
                     /locus_tag="AC1ZCC_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630910.1"
                     /translation="MGDMGEYWKDIKPHLKERRKQHVRKMGNSATKNIETLGFEFTHY
                     PNNHQFAINTHNGIIDYWGTTGTWIERKTKKRGKGIRSLRKYINQEDSK"
     gene            1057..1383
                     /locus_tag="AC1ZCC_002703"
     CDS             1057..1383
                     /locus_tag="AC1ZCC_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630911.1"
                     /translation="MNYTQQELTDLCPKHVAEFINNEVLPKYADGLNTAENVTDFMIN
                     DAIDRLIFLEIDCIAYYRLHAEVALIDPYIALSQNRKILVAYIDTKQILEGRDVEIEI
                     DEEEVG"
     gene            1380..1577
                     /locus_tag="AC1ZCC_002704"
     CDS             1380..1577
                     /locus_tag="AC1ZCC_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630912.1"
                     /translation="MKAYTIKFYGNKTYVYILSGLRLDFRGYSKEDLLESLYGYGYLN
                     EDELISLEKFTNAWTIEEEAE"
     gene            1574..1783
                     /locus_tag="AC1ZCC_002705"
     CDS             1574..1783
                     /locus_tag="AC1ZCC_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630913.1"
                     /translation="MKIYVVKFGNKFYRSDERSIGANTLFIVDILQSARWFDSLEEAN
                     QVSRRLGGLTQVYELVTVDHEEVER"
     gene            1780..2067
                     /locus_tag="AC1ZCC_002706"
     CDS             1780..2067
                     /locus_tag="AC1ZCC_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630914.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKKRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMKAI"
     gene            complement(2100..2351)
                     /locus_tag="AC1ZCC_002707"
     CDS             complement(2100..2351)
                     /locus_tag="AC1ZCC_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630915.1"
                     /translation="MTMWSMLLFWIPVCIGIVAFCYFVKHSRTNKLLMLSFLPIVFFI
                     VQIVKYTYIESQEIFIFYVVGLFISVVFFIMILSYFYKK"
     gene            2659..>2966
                     /locus_tag="AC1ZCC_002708"
     CDS             2659..>2966
                     /locus_tag="AC1ZCC_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HIQ1630916.1"
                     /translation="MVLFDVKKYETPEAKDVDMDRTKHNVGVFLSAYLSARCRIGQPR
                     EPKVTASYSLVPPSTANNTFEAERMMIEREEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     T"
BASE COUNT         1088 a          436 c          622 g          820 t
ORIGIN      
        1 gatcgtttca aatttgtaga aaagtataga gagatactga atgagtattt cgaggaggac
       61 tagatgttta attcaacgtt agcgtcaatt cactttttat tctatataac gatatttgta
      121 gctatattaa ttaatgcgat aaaaagcggt tttttaacac caatcggcac aattttaaca
      181 ttagcttcat ttgttacaag ttgtattatc caacttaaat atataaagga gaatgaataa
      241 tggacgaact aatcacaaaa gtagagcagt gggctaaaga taagggattg gatcaagctg
      301 attccagcaa gcaaatgtta aaaacgatcg aagagatcgg ggaagttgcc gcttctctag
      361 ctagaaaaga tgaacatggt ttaagagacg gaattggaga tgtagtagta accttgatta
      421 ttttagctat gcaaaatgat atggatttgt acgagtgtct gaaccaagca tacaacgaaa
      481 tcaaaggacg cacaggaaaa atggtagatg gtgtattcgt gaagtcgagt gatttggagg
      541 acagcaaatg atcagcctaa caatattttg gtgtgtaatt ggcggcttac tagcgatgtt
      601 aggagcatac ctcatttacg agagtgcaaa aggcaaaaaa tatacagcag tagggattat
      661 tttgtttgtt gtcggaattt tagttgcgat tggtgcagaa ataaatcttt atttaaacgg
      721 tacgacagaa gagttgatgt ggtttttatt gtggacaagg agataggagg acagcgaaat
      781 gggcgatatg ggcgaatact ggaaagatat taaaccgcat ctaaaagaac gtagaaaaca
      841 acatgtcaga aagatgggca attcagcaac taaaaatatt gaaaccttgg gatttgaatt
      901 tactcattat ccaaacaatc atcaatttgc aattaatacc cataatggaa ttattgacta
      961 ctggggaact acaggaacgt ggattgagcg caaaacaaaa aaacgtggta aagggatacg
     1021 tagtttgagg aaatatatta atcaggagga cagcaaatga actatacaca acaagaactg
     1081 actgatttat gtccaaaaca tgtagctgag ttcatcaata atgaagtcct gcctaaatat
     1141 gcagatggtt taaatacagc agaaaacgtt acagatttta tgattaatga tgctattgat
     1201 cgtttgatat ttttagaaat agattgtatt gcttattatc gtctacacgc agaagttgct
     1261 ttgattgatc cgtatattgc tttaagccaa aatcgaaaaa tactcgtagc ctatattgat
     1321 accaaacaga ttttagaagg cagagatgtc gagatagaga tcgatgagga ggaagtggga
     1381 tgaaagcata cacaatcaag ttttatggaa ataaaaccta tgtatacatc ctaagcggtc
     1441 tcagacttga ttttagaggc tattcaaaag aagacctttt agaatcctta tatggttacg
     1501 gatatttaaa tgaagatgaa ctaataagct tagagaaatt tacaaatgct tggacgattg
     1561 aggaggaagc agaatgaaga tttatgtagt aaagttcggg aataaatttt atagaagtga
     1621 tgaacgctct ataggggcta acacattatt cattgtagac atactccaaa gtgcgagatg
     1681 gtttgatagc ctcgaagaag ctaaccaagt ttcacgacga cttggtggat taacacaagt
     1741 atatgaactg gtcactgtcg atcatgagga ggtagaaaga tgaaattaaa agacggattt
     1801 tacgctagta gtcatggtat cggcggttta atgctagata tgccgacaaa gaatcctaaa
     1861 aaacgtaaga aaccaaaatt caaagtcggt gacatggttc gctgcgaagc agaagggttc
     1921 atctatccat ttcgtggata tgtagaacac gtctataatc actcagcgat cattcgtatt
     1981 gaaaacacga tggaatgtga taagtggtta gcgaaaagca aagagaattt agctgtagcg
     2041 agattggtgg atatgaaggc tatataaaca gcgaaaaaat gtacttctaa gagaaaaatt
     2101 tattttttat aaaaatagga aagtatcatt atgaaaaaga ccacagagat aaataaacct
     2161 accacataaa aaatgaatat ttcttgcgat tcaatatagg tatatttaac aatttgtaca
     2221 ataaaaaata ctataggtaa aaaagataac atgaggagct tatttgttct agagtgtttg
     2281 acaaagtaac aaaatgcgac gataccaata caaacgggaa tccaaaataa cagcatgctc
     2341 cacatagtca ctcaaccatc tcctatacgc tttattatca atagataata tcatttttcg
     2401 taataaagat aaaaaaataa gtaaggcttc tcattaaaat ataaaaaaga cagccgacca
     2461 ctggctgtcc ttagcagaat attgaaatag tgagccatct gttttccgcc agatagctca
     2521 acatgtatat gggtaacgag tgcaacctcg ctacttgaaa agcttagcat aagcatgggt
     2581 ataaaaaaag ccggattcct ccgaccttaa ttaataattc tgacacaatt attatatcat
     2641 aaatggagga atcaatggat ggtacttttc gatgtaaaga agtatgagac accagaggca
     2701 aaagatgtgg atatggatcg cacaaaacat aatgttggtg tttttctttc agcatatctg
     2761 tcagctagat gtaggatagg tcaacctcgg gagcctaaag taacggcatc ttactccttg
     2821 gttccacctt ctacagcaaa taatacattt gaagcagaac gaatgatgat tgagagagag
     2881 gaagcgcaag aagaatttga gtacttgcac aaattatttg ttcgtggcta ttcagcaatt
     2941 cagcatccgc ataaacctga tgtaac
//
LOCUS       DBNICI010000216         2963 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.220, whole genome shotgun sequence.
ACCESSION   DBNICI010000216 DBNICI010000000
VERSION     DBNICI010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.220"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(84..1031)
                     /locus_tag="AC1ZCC_002709"
     CDS             complement(84..1031)
                     /locus_tag="AC1ZCC_002709"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIQ1630917.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1277..1741
                     /locus_tag="AC1ZCC_002710"
     CDS             1277..1741
                     /locus_tag="AC1ZCC_002710"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIQ1630918.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1803..2846)
                     /locus_tag="AC1ZCC_002711"
     CDS             complement(1803..2846)
                     /locus_tag="AC1ZCC_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HIQ1630919.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          979 a          586 c          566 g          832 t
ORIGIN      
        1 tttattcgga aaaaaggact gtgacaagac ttttgtcaca gtcctttttc atttaaaaaa
       61 atgtttactt catttgacgt atattattgg tgaggcttca attcatttgg cttcactttt
      121 tctgtttctc ttatgatata aacaggacgc tgtttcgttt ctaagaagat tttcccaata
      181 tatttcccga tgattccaag acataacaat tggattccac caatgaacag gaagatcgag
      241 accatggaag gccatcctga tgtaggatca ccaaacaaca atgcccgcaa aataatgaac
      301 aaaattgcaa taccagaacc gacacaagac aaagcgccga taaatgaagc aatcgttaac
      361 ggtgcatccg agaagttcac gataccatca atcgagtagt tgaataattt ccagaaggac
      421 caagatgttt cgccagccac acgttcgcgg ttttcataag aaatatattc tgttttaaat
      481 ccgacccagc taaataatcc ttttgaaaat cggttgtatt ctgttaattc taggacggca
      541 tccaccattt gacgagtcat caagcggaaa tcacgagcgc catcgaccat ttctgtatcg
      601 ctgattttat tgatcaactg gtagaatttt ttcgcaaaga aactgcgtat cgcaggctcg
      661 ccttcacgag tcgaacgccg ggtgccgaca caatcgatat cagaagtagt gatccggttg
      721 atcatctcag gaagcagttc gggcggatct tgcagatcaa catccatcac ggtcactaaa
      781 tctcctgtag aagcttgcaa tccagcgtac agagccgctt ctttcccaaa atttctggaa
      841 aatgagacaa agcggacata aggataagct gaggctagct tcttgattgc tgctaacgta
      901 ccgtcttttg atccgtcgtc aataaacaaa tattcgaact catgggcacc tttgaatttt
      961 tcaacctcat caaaaaaaag aggaacagtt ttttcttcat taaaacatgg gacaacaatt
     1021 gatactttca tcgtttctat cctttcaatc gtatgtttat atatggttta cattcaaaat
     1081 ttaataaacc ttaaataaca cctttaaaat ttgattttac gtaaaagaac aggtattctt
     1141 tttgtaaact aacgtattta gtttaccatg tttcctcggt ttatcaaact tttaagaaca
     1201 atttttatta gttggcaagt agttttggat agagaaaaag gaggaagttc cagtctattg
     1261 actaggagca aacaaaatga aaatacacac acctatcggc tttttatggt tagatgcaga
     1321 tgaatccgga ttgactgctg tatcttttca gccaatttca gaaagcgaat cagcgaatca
     1381 gcgaatttta acgcgcacac aagaacagtt tgaagaatat tttgcgggaa aaagaaaaga
     1441 atttgatgtt cctttatcta taaagagagg tacgcctttt cagcaaaatg tctggaaagc
     1501 tttgtcgacc attccttatg gagaaatccg aacgtataaa gaagtagccg ttgctgttga
     1561 tagtccgaaa gctgtgcgag cgattgggca ggcaaatcga accaatccat tgccaatcgt
     1621 tattccttgt catcgagtga tcgggcaaaa tggaaaactg acaggatata tgggaaacgc
     1681 aatagatggg gttgaaatca aacgccaatt gttgttgtta gaaggatatc tgcaaaatta
     1741 aaaacagttg tgattcggac attgttccat gcgagaatca caactgtttt ttcatgttca
     1801 ttctaaactt tgatcagcca aataaatagt aaagccatca ctgtttggag aagcccgaca
     1861 gctaatccat aagataagaa gcgtgtacct tctttcatga attttgcaaa gtctaaacgc
     1921 agaccaattg ccgctaaagc agtgatctca aaccagctgc tgataaaatg tgccgattgg
     1981 tcaataacta caggtaaaga aaacaagctg ttgaccaaac aaaagatcac aaaccctaat
     2041 acataccaag gaagcggtaa ttttcgtttt ttctgcgtat cagttggcgt ttcaaacaga
     2101 gctggttgtt tcttgtgcat gaatttttca aacagataga caacaacaac gagcatcata
     2161 atacgtagaa tcttaaatag catggcgaat tcgacaacag aattgtcaag taagcttgcc
     2221 ccagcgacta cttgaccaac agattggagt gtaccaccca aaagtgcact tttttcaatg
     2281 agatttccac caaacaactg taatccaaga aaagggagag tcaacatcat cacagtaccc
     2341 agtagattga ctagtgtgat gatctgtcct ttttcttcct ctttcgcatg gatggaaggg
     2401 gcgattgaag caatagcaga agaaccacaa acagcgtttc ctccagccat catcaaagaa
     2461 gaagtatcgc tgaattgcag ttttttacca atcaagtaag caccgatgat agtgactgac
     2521 atttgcagta acacaaaaac aattcctttt acaccaattt gagcaatcgt ttgagctgtg
     2581 acagttgcac ctagtaaaaa aacagagaat tctaatagtt tgctttcagc aaattttgtg
     2641 ccgattgcta gaacaggctg tttaaaaaaa gtatttccta ataaaatacc caataaaata
     2701 gcaatagtcg ctccacctaa ctgtggcaga taaatagcaa gaagcctgct gaaggcagcg
     2761 atggcaaaag aaagcagtaa accgggtaag atttcgcgga ccgctgaatc ctttacagat
     2821 tttattggat gtgacaatga tttcatttga tcggttcctt tcgtcaataa atactaaaag
     2881 catactcgaa catagtagca ctaaaccagt attttgacaa aagaacaaga ggtcgtgaca
     2941 aaagtcttgt acgacctctt gtt
//
LOCUS       DBNICI010000217         2918 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.221, whole genome shotgun sequence.
ACCESSION   DBNICI010000217 DBNICI010000000
VERSION     DBNICI010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.221"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            205..1173
                     /locus_tag="AC1ZCC_002712"
     CDS             205..1173
                     /locus_tag="AC1ZCC_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HIQ1630920.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1166..2197
                     /gene="vanA"
                     /locus_tag="AC1ZCC_002713"
     CDS             1166..2197
                     /gene="vanA"
                     /locus_tag="AC1ZCC_002713"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HIQ1630921.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2203..2811
                     /locus_tag="AC1ZCC_002714"
     CDS             2203..2811
                     /locus_tag="AC1ZCC_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HIQ1630922.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          863 a          559 c          708 g          788 t
ORIGIN      
        1 taatttttta ggaaaatctc aaggttatct ttactttttc ttaggaaatt aacaatttaa
       61 tattaagaaa cggctcgttc ttacacggta gacttaatac cgtaagaacg agccgttttc
      121 gttcttcaga gaaagatttg acaagattac cattggcatc cccgttttat ttggtgcctt
      181 tcacagaaag ggttggtctt aattatgaat aacatcggca ttactgttta tggatgtgag
      241 caggatgagg cagatgcatt ccatgctctt tcgcctcgct ttggcgttat ggcaacgata
      301 attaacgcca acgtgtcgga atccaacgcc aaatccgcgc ctttcaatca atgtatcagt
      361 gtgggacata aatcagagat ttccgcctct attcttcttg cgctgaagag agccggtgtg
      421 aaatatattt ctacccgaag catcggctgc aatcatatag atacaactgc tgctaagaga
      481 atgggcatca ctgtcgacaa tgtggcgtac tcgccggata gcgttgccga ttatactatg
      541 atgctaattc ttatggcagt acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat
      601 gatttcaggt tggacagcga ccgtggcaag gtactcagcg acatgacagt tggtgtggtg
      661 ggaacgggcc agataggcaa agcggttatt gagcggctgc gaggatttgg atgtaaagtg
      721 ttggcttata gtcgcagccg aagtatagag gtaaactatg taccgtttga tgagttgctg
      781 caaaatagcg atatcgttac gcttcatgtg ccgctcaata cggatacgca ctatattatc
      841 agccacgaac aaatacagag aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt
      901 ccacttgtag atacctatga gttggttaaa gcattagaaa acgggaaact gggcggtgcc
      961 gcattggatg tattggaagg agaggaagag tttttctact ctgattgcac ccaaaaacca
     1021 attgataatc aatttttact taaacttcaa agaatgccta acgtgataat cacaccgcat
     1081 acggcctatt ataccgagca agcgttgcgt gataccgttg aaaaaaccat taaaaactgt
     1141 ttggattttg aaaggagaca ggagcatgaa tagaataaaa gttgcaatac tgtttggggg
     1201 ttgctcagag gagcatgacg tatcggtaaa atctgcaata gagatagccg ctaacattaa
     1261 taaagaaaaa tacgagccgt tatacattgg aattacgaaa tctggtgtat ggaaaatgtg
     1321 cgaaaaacct tgcgcggaat gggaaaacga caattgctat tcagctgtac tctcgccgga
     1381 taaaaaaatg cacggattac ttgttaaaaa gaaccatgaa tatgaaatca accatgttga
     1441 tgtagcattt tcagctttgc atggcaagtc aggtgaagat ggatccatac aaggtctgtt
     1501 tgaattgtcc ggtatccctt ttgtaggctg cgatattcaa agctcagcaa tttgtatgga
     1561 caaatcgttg acatacatcg ttgcgaaaaa tgctgggata gctactcccg ccttttgggt
     1621 tattaataaa gatgataggc cggtggcagc tacgtttacc tatcctgttt ttgttaagcc
     1681 ggcgcgttca ggctcatcct tcggtgtgaa aaaagtcaat agcgcggacg aattggacta
     1741 cgcaattgaa tcggcaagac aatatgacag caaaatctta attgagcagg ctgtttcggg
     1801 ctgtgaggtc ggttgtgcgg tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga
     1861 ccaaatcagg ctgcagtacg gaatctttcg tattcatcag gaagtcgagc cggaaaaagg
     1921 ctctgaaaac gcagttataa ccgttcccgc agacctttca gcagaggagc gaggacggat
     1981 acaggaaacg gcaaaaaaaa tatataaagc gctcggctgt agaggtctag cccgtgtgga
     2041 tatgttttta caagataacg gccgcattgt actgaacgaa gtcaatactc tgcccggttt
     2101 cacgtcatac agtcgttatc cccgtatgat ggccgctgca ggtattgcac ttcccgaact
     2161 gattgaccgc ttgatcgtat tagcgttaaa ggggtgataa gcatggaaat aggatttact
     2221 tttttagatg aaatagtaca cggtgttcgt tgggacgcta aatatgccac ttgggataat
     2281 ttcaccggaa aaccggttga cggttatgaa gtaaatcgca ttgtagggac atacgagttg
     2341 gctgaatcgc ttttgaaggc aaaagaactg gctgctaccc aagggtacgg attgcttcta
     2401 tgggacggtt accgtcctaa tcgtgctgta aactgtttta tgcaatgggc tgcacagccg
     2461 gaaaataacc tgacaaagga aagttattat cccaatattg accgaactga gatgatttca
     2521 aaaggatacg tggcttcaaa atcaagccat agccgcggca gtgccattga tcttacgctt
     2581 tatcgattag acacgggtga gcttgtacca atggggagcc gatttgattt tatggatgaa
     2641 cgctctcatc atgcggcaaa tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg
     2701 cgctccatca tggaaaacag tgggtttgaa gcatatagcc tcgaatggtg gcactatgta
     2761 ttaagagacg aaccataccc caatagctat tttgatttcc ccgttaaata aacttttaac
     2821 cgttgcacgg acaaactata taagctaact ctttcggcag gaaacccgac gtatgtaact
     2881 ggttcttagg gaatttatat atagtagata gtattgaa
//
LOCUS       DBNICI010000218         2847 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.223, whole genome shotgun sequence.
ACCESSION   DBNICI010000218 DBNICI010000000
VERSION     DBNICI010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.223"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..572
                     /locus_tag="AC1ZCC_002715"
     CDS             <1..572
                     /locus_tag="AC1ZCC_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HIQ1630923.1"
                     /translation="IRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIK
                     GRKIYLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQY
                     QMPQYQKKLKENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLN
                     EYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
     gene            594..1838
                     /locus_tag="AC1ZCC_002716"
                     /pseudo
     CDS             594..1838
                     /locus_tag="AC1ZCC_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620473.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            1875..2717
                     /locus_tag="AC1ZCC_002717"
     CDS             1875..2717
                     /locus_tag="AC1ZCC_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HIQ1630924.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
BASE COUNT         1014 a          434 c          585 g          814 t
ORIGIN      
        1 aaatccgaat aaagcgatat aaatcttata aaggaacagt aggaaaaatt gccaagaatg
       61 tgttaaaacg aaatttttca gtagatacac ccaataaaaa atgggtgaca gacgtcactg
      121 aattcaagat aaaaggaaga aaaatctatc tatctcctat acttgattta tttaacggag
      181 aaataattag ttatagtata tcaactagtc caacatataa actgattgaa gaaatgcttc
      241 aacaagcaat taaaaaaaag ggaactgagg gttcactgat tctacattca gatcaaggat
      301 ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga gaataacatt attcaaagca
      361 tgtctagaaa aggaaattgc ttagataatt ctgtaataga aaattttttt ggtgtgctta
      421 aatcagagtt tttttatcga gaaaaattcc gatcaattga gatatttcaa agtaaattaa
      481 atgagtatat taggtggtat aataacaaaa ggataaaact aaagttaaat ggactatctc
      541 cagtagaata tcggaaacag tccattaaat agttcaactt ttggggttca gttcaaagaa
      601 ggggcgttga tttttatcat cactataaag aagatttagc tttatttgcc gaaatgggct
      661 ttaaaacctt gcgcctctct attgcttgga ctcgcttgtt ccctacagga gaagagctgg
      721 aaccgaatga aaaaggggtt gcattttatg aaaacgtctt taaagaaatg caacgtttag
      781 gaattgagcc gattgtgaca ctatctcatt atgaaatgcc attagcttta gcagttaaat
      841 ataatggctg ggtggagcgt cgagtaattg atgattttgt tcgcttcgct aacgtttgtt
      901 ttgatcgctt cggcaaatac gtaaaatatt ggctgacatt taatgagata gacagcatcc
      961 accgtcatcc atttacaaca gctggaatta tccaagagaa aagcgcagaa ggtcaagaag
     1021 ctcaagatat ctatcaagca ttgcatcatc aatttgttgc gagtgcgtta gtgacaaaag
     1081 atgctcatga aaaaattcca ggaagccaag ttggttgtat gttaacgaaa ttgatgactt
     1141 atccattaac ttgcgcacca gaagacgttg agctgacctt gaaaaagaac ttagaaaata
     1201 atttttatgc agatgttcaa gttaagggag aatatcctgc tctaatcgaa aaggatttgg
     1261 aaaatcgggg aattcagatt aaaatggaag atgaagatcg agaaatttta aagaaacata
     1321 ccgtagattt tgtttctttc agctattaca tgtccatggc agaatcagct gatccaaacg
     1381 cagaacgcac accgggaaat actgttttag gggttaagaa tcctcatttg ccttcaacag
     1441 attggggctg gcaaatcgat ccaaaaggtt tgaaaatttc gttgatcgaa ctttatgatc
     1501 gttaccaagt accgttgatg attgtggaaa atggtatggg agcagtcgat caggtagtag
     1561 atggtaaaat ccatgatccg taccgcatcg actactttaa agcacacttc caacaaatga
     1621 aagaagcaat cgatgaaggt gtagatttga tagcttatac tagctgggga ccaattgatt
     1681 tagttagtgc aggaacttct caaatgtcta aacgatatgg atttatttat gtggatgcag
     1741 acgatgaagg aaatgggaca tataaccgct tgaaaaagga ttcgttcaac tggtacaaag
     1801 aagtgatcga aacaaacggt gcatccttat tcgagtagtc tcgatctttt taagcagcga
     1861 tagggggata atgagtgatt tcaataaaaa aagtattgaa ttcaagtgtg gtacttgttg
     1921 aagaaaatgg caaagaaatg attgcattag gtaaagggat aggctttgga cagaaagctg
     1981 gggatgaaat atcaggagat aaagtagata aaatatttct gccttttgaa gaaaagaaat
     2041 cttcacaact attagagtta gttgaagaaa tacctattga attttttgaa atcactcgta
     2101 ctatcgtttt tgaagcagaa aaaggattag ataaaaaact gaatcctgga gtctatttaa
     2161 ccttaacaga ccacctgcat tttgcagttg aacgcgcaca aaaaggggta aatattgcaa
     2221 accgtttgta ctgggaaata aaaatctact atcccaaaga atttcaaatt gggaagtacg
     2281 cactcaccct tttgaacgag caatatggaa ttgatcttcc taaagaggaa gcctcaaaca
     2341 ttgctttcca tttgatcaat gcacaatcgg aagatcctga tagcggcgct ggattcaagt
     2401 atgcaaaaat gatcggcggg atcgtcaatt tggtccggta ttcgttgcag aaagatatcg
     2461 atactaattc gatccattat gcccgcttca ttacacatgt acgtttcttt gtagaaagat
     2521 attattctga tgctttgttg gatgatggtg aggaagaatt ctacaatcaa atgtggatct
     2581 tatacccgtc tgccatggaa agcgcagcta agatcaaaga ctacattgaa aaagtttatc
     2641 ataaaacaat tccgaacgat gaagttgtat acctaggtgt ccacatcaat cggttgatga
     2701 aacatgctaa tgggtgagcg ctcagtaata ctattgatgc ttttaaaatc tttagagatg
     2761 gattatagta aaaacaaggt gatttccagc tgtttgttat ctataagaga catttaaaaa
     2821 ggattaacgt aagataataa aaaatct
//
LOCUS       DBNICI010000219         2823 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.224, whole genome shotgun sequence.
ACCESSION   DBNICI010000219 DBNICI010000000
VERSION     DBNICI010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.224"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            136..309
                     /locus_tag="AC1ZCC_002718"
     CDS             136..309
                     /locus_tag="AC1ZCC_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630925.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            949..1422
                     /locus_tag="AC1ZCC_002719"
     CDS             949..1422
                     /locus_tag="AC1ZCC_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HIQ1630926.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="AC1ZCC_002720"
     CDS             1431..1658
                     /locus_tag="AC1ZCC_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1630927.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="AC1ZCC_002721"
     CDS             2294..2665
                     /locus_tag="AC1ZCC_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630928.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT          904 a          456 c          417 g         1046 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atg
//
LOCUS       DBNICI010000220         2819 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.225, whole genome shotgun sequence.
ACCESSION   DBNICI010000220 DBNICI010000000
VERSION     DBNICI010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.225"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            240..656
                     /locus_tag="AC1ZCC_002722"
     CDS             240..656
                     /locus_tag="AC1ZCC_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HIQ1630929.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            670..1236
                     /locus_tag="AC1ZCC_002723"
     CDS             670..1236
                     /locus_tag="AC1ZCC_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HIQ1630930.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            1259..2245
                     /locus_tag="AC1ZCC_002724"
     CDS             1259..2245
                     /locus_tag="AC1ZCC_002724"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIQ1630931.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(2400..>2819)
                     /gene="scm"
                     /locus_tag="AC1ZCC_002725"
     CDS             complement(2400..>2819)
                     /gene="scm"
                     /locus_tag="AC1ZCC_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HIQ1630932.1"
                     /translation="STDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT          848 a          523 c          591 g          857 t
ORIGIN      
        1 ttgaaaagta attttcgtaa attccttctt atttcttgaa gttgcccact tctgtcacaa
       61 cctcttgtta taaacggtgt tccacctgct gcttctcggt aataagccca aagaattaga
      121 aaacatggaa agctgttttc tattcttcgt tcttattact caaagcaaga cacaggtgtc
      181 acaacctctt gtttttatgc taaaattggg tgagttaatc tggaaaaagg agaacaaaaa
      241 tgagccttaa agaatttgct gaaaaaagtc tgctcaaacc ttgggatgtt tttattattt
      301 tgcttttgac tgttctttct tttttaccag tcatgatttt tggtcttcag caaaacaccg
      361 caacagcaga aaaagaagcg gtactgcgtg tggatggcac agaaatcaaa acattccctt
      421 tgatggctgg agaagagagc aacacatata cttacaaaga tgaacacgga gattataact
      481 tgatcgagat agatggcgat cgagtacgga taaaagaagc agattgcgat gaccagatct
      541 gcgtacgtcg aggctgggca acgaaaaatg gcgaaaccat cgtctgtttg ccacataaat
      601 tagtgatcga agtccgttca acagatggag gtgacgaaga cagtctgatt tattagacct
      661 ttacacacca tgagtaaatt acaaaaaatg atttatattt cgctacttgt tgcacaagga
      721 gtgatcattg gtttattgga aaatatgatc ccttatcctt tttcttttgc gcctggtgca
      781 aaactaggct tagccaatct aatcacgatc gttgccattt ttaccatgaa aaaacaagac
      841 agcttcttac tactttggct acgactattc ctgactactt tgctaggcgg aacgatttcc
      901 acattccttt atagtatgag tggtgctttg cttagttatt ttggaatgtt attggtcaag
      961 caattaggac caaaacgtgt cagtatcatc ggaattagtg cggccggcgg tttcatgcat
     1021 aatgtcggac agcttttgat ggcttctttc attgcacaat catggacagt catgttgtac
     1081 ttgccagtcc tttcagtttt agggatttta tcagggattg caatcgggat cgcagcgaat
     1141 tatcttttac gacatgtgca gacacttcaa cgattccaaa aagattatga gcagggaacc
     1201 aaacaaaaat ggagcccaat gtttaccaaa aaataatcgt cacacaagga gtttttttat
     1261 gaagctacat ccaatgtggg aaaactatcc tgctttaagt aaagaattga cagaaacact
     1321 aaaacggatg gaagaaactg tccgcctgaa aaacaaacct gtcgaacaag caatcaaaga
     1381 aacgatccat gccggcggaa agctgttgcg tcctgcctat cagctattgt tctcacaatt
     1441 tggaccagag caagatcgag acaaagccat cgctttggca gcttctatcg aaatgctcca
     1501 tacggccaca ctgatccatg atgatatcgt tgatgaagcg gatctgcgac gtggtcagcc
     1561 taatatccgt agtcagtttg gtaatgtcgt ttctgtctat gcaggagact atttatttgt
     1621 ctgctgtttt aaactattgt ctgattactc cacttccttg aaaagtctcc aattgaattc
     1681 tcgcagtatg gaaaaagtat tgaatggcga attagggcag atggatgatc gctataatta
     1741 tgaactttct gtaaaagaat atttggaaaa tatttcaggt aagacagctg aactgttcca
     1801 attaagttgt tctgttggcg cttttgaaag tggaacaagc gagcgcttcg caaaaaaagc
     1861 aggagatatc ggactttcta tcgggatggc ctttcagatt atcgatgata ttttagatta
     1921 tacaaaagag agccaggaaa tcggcaaacc tgtactagaa gacatgcgtc aaggggtcta
     1981 ttctttaccg ttgatctact ctttgcaaaa aaacaaacca aagctactac cttatttaga
     2041 gaagaaagcg gcaatgacag aagaagatgt cgacgccgta cgcaaaatcg ttgaacatac
     2101 aggaggcgtg gaagaagcta ggaagttagc ggcctcttac acagaaaagg ctttgaagga
     2161 aataaagaaa ttacctgcca cctctcttcg aacaaaagag aatctctttt cccttaccca
     2221 actgattttg gatcgcaaag actgaatagg gaaaggttcg atacgtaaat aaatttctca
     2281 gaaggaaatc tacgtgtttt ttgacaaatt gaattgtagt tcttgtattt atgataaaaa
     2341 gcaaaaataa agagcaacgt ccgttagcga cgttgctctt ttttatcttg aatatttttt
     2401 taccttttcg atttcttata actcataaaa gtgattctta aaccgattac aagtaatata
     2461 acgcctgcga gtgttatcca attattcgat tgacttccag ttttaggaag tgctcttttt
     2521 gatttgtttc cattgtcatt gcttgtttta ttagttaagt taaagttcgt cctgttatcc
     2581 gaacttgttt ttgagaagga gacacttgca tgacgtgtat cgcttgtggt agacgacgtg
     2641 tctgtactat cgcttgaagt tgatgaatct gagctatcac ttgtggtaga ggatgtatct
     2701 gtactgtcac ttgaagctga cgtgtccgtg ctatcacttg tagtggtaga tgaatctgag
     2761 ctatcacttg tggtagaggt agtatctgta ctgtcacttg aagctgacgt gtccgtgct
//
LOCUS       DBNICI010000221         2804 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.226, whole genome shotgun sequence.
ACCESSION   DBNICI010000221 DBNICI010000000
VERSION     DBNICI010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.226"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..1148)
                     /gene="ltrA"
                     /locus_tag="AC1ZCC_002726"
     CDS             complement(<1..1148)
                     /gene="ltrA"
                     /locus_tag="AC1ZCC_002726"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HIQ1630933.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQK"
     gene            complement(1894..2031)
                     /locus_tag="AC1ZCC_002727"
     CDS             complement(1894..2031)
                     /locus_tag="AC1ZCC_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HIQ1630934.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(2028..>2804)
                     /locus_tag="AC1ZCC_002728"
     CDS             complement(2028..>2804)
                     /locus_tag="AC1ZCC_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HIQ1630935.1"
                     /translation="GCRQFETFLLAQKRSWYDFFEDCLKAGGVMKRLDLAINDRVGLL
                     DIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGMGNTLYIGSLKSEVYFCLYEK
                     DYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLLKGRSIESTTFSIINRYLRFA
                     DKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYTIERTLNWLGRQVAPTWKMAK
                     ELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENLII"
BASE COUNT          716 a          621 c          435 g         1032 t
ORIGIN      
        1 tctttttggt ttttgaattg tttatcactt atatgtgatt taacaactaa cttctttctt
       61 ctcggtatca ttttaatctt gaaaccaaga aagtcggagt atctttttct aacatttaca
      121 attcttgttt tttcttgtga tacttccaat tttaatcgtt gttcaatcca ttgagtgatt
      181 gctattttgg ctttttcagc actttccttg tagcggcaga aaactctaaa atcatctgca
      241 taacgaacca tatacatttc ctttaacttt gttttcttca tttctttgta agcgttgctt
      301 ttacatgggc taccgctttt attgatatga accacatagt tttttgtaac aggattttcc
      361 tgccattggc tttctaccca atggtctaac tcattaagaa caatgtttgc tagtagtggg
      421 gatattattc ccccttgcgg agttcctttc tctggatatg ttattgtacc gttagtcatt
      481 ttgattggag cttttaatat cctttttaaa atgaatatca agtgcttgtc ccgtattccc
      541 atagcccata tctgttttat cagcttggaa tggttcacgt tgtcaaagaa acccttaata
      601 tcaaattcaa tcacataatg tagctggctg atttgtaggc ggttataggt tgcttttatt
      661 gcattttcaa ctgacctatt aggtctaaaa ccatagctgt tttcactaaa tttagcttcg
      721 cagattggtt ctaatacttg ttttatacat tgttgtataa gcctgtccca catacaagga
      781 atacctagag gtctggtttt accatttggt tttggtattt cttttcgcct cactggctta
      841 ggtcgatacc catgtggact gcctgcaaca atgtatctta cttttttgac aactgtttct
      901 gctggcaatt tgccaatatc tttaattatt gtaccgtctg ttcctgctgt tttactgcct
      961 gcatttgctt tgatatttcg atatgccagt aatatatttt ctctcgatag gataatatcc
     1021 ataagatgag tgaaaatatc tccattctta cttttggcat ataaatcatc aaaggtgttt
     1081 tgcatttcgt aatattcagc gtgtcttaaa tcttctacac acaatgtttc atttttattt
     1141 ttaggcataa ggcttcactc ctttacttga aattgagtga gcttttttat cttactcgta
     1201 atccttattg aaacaacata atacttttct aatatataca cgacttgagg gcattcctcc
     1261 attctcatta caagaatttc ttaggttcgc cctctgcttt tcagcattag ttcaactgct
     1321 tattgttctt cgtcagttta ttctcttgat gagaacccaa tacctttcct cgttccgata
     1381 atcttatcta tacatatata tccttaggtc actagctata agcctgctgg cttgataccg
     1441 cctgtaacgt tatatggcat ttcataacct atattcttac tttgccacac cagctacgat
     1501 ttctcgtacc tttgcatttc tacaaagttc aaatttagac ccgtacattc gctgtttcgt
     1561 cagttcctta tagaacattc taaccataga tattttatag acccccgacc tgttggatac
     1621 gctacccacc tctggatagt tttcgataga agatatatgt tcatcaacta ttacggtatc
     1681 tttcagccgt ctttaccgag cttcaaacat tctgagtttc ctcatacaat gcttgtcgga
     1741 ggctcagggc aggcgtttca gagcgttact tcatcattcc acctgtcaga ttatcagttc
     1801 tcctaataac caaacttttt cgttctttta ttagtgcctt agcttttcac taaggaacga
     1861 gtcgcacata aattttgtcc gaaattcatc ttgtcacttt cctttctttt ccattgctcg
     1921 atccgctgct gcacttgcat tcatcaagca catcacagta acaccaagca tactgccacc
     1981 aaataccaaa aaaatatctt ttacaattcc catgacgatt cctcttttca tataatcaaa
     2041 ttttcaattg ccatactttg ttgctccaaa atctttttat gtcggtctga taagcgtgca
     2101 ttctgtacca tatcctggat ataggttgtc cgattcaatc gatccaactc cttagccatt
     2161 ttccacgtag gggcgacttg tcggcctagc caattcaagg ttcgttcaat ggtatacggt
     2221 tttggctcgg aagttaattg aatctctttc cgattccgtc caataaaacg cccccattgt
     2281 tcattcaagg gccagttggt tcgcctttta ccttctactt tatccgcaaa tcgcaagtaa
     2341 cgattgataa tggaaaacgt ggtgctctca atgctacgac cttttaacaa atcttgaatc
     2401 gcatgaaaag cacggtcatt tttcagtcga atctcaaaac gatttttcgt ctcagtttgg
     2461 tctaatggaa tccctaactt gatatattgt tcatagtctt tttcatacaa acagaaataa
     2521 acctcactct tgagactgcc gatatataag gtattgccca tgccgtcctt ttcatcagcc
     2581 tttaaaagtt ccccggaacg ataactttta aaggtacgaa acaaggagat acattcttcc
     2641 ttctgacatt ttttcgttag ctcaggtata tccaatagcc ccactcgatc attgattgcc
     2701 aagtccaagc gtttcatcac gccaccggcc tttagacaat cctcgaaaaa gtcgtaccag
     2761 ctacgctttt gagcgagtaa aaaagtttca aattgtcggc aacc
//
LOCUS       DBNICI010000222         2724 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.229, whole genome shotgun sequence.
ACCESSION   DBNICI010000222 DBNICI010000000
VERSION     DBNICI010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.229"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            89..406
                     /locus_tag="AC1ZCC_002729"
     CDS             89..406
                     /locus_tag="AC1ZCC_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HIQ1630936.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(497..862)
                     /locus_tag="AC1ZCC_002730"
     CDS             complement(497..862)
                     /locus_tag="AC1ZCC_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630937.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1070..1552
                     /locus_tag="AC1ZCC_002731"
     CDS             1070..1552
                     /locus_tag="AC1ZCC_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630938.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1749..2639
                     /locus_tag="AC1ZCC_002732"
     CDS             1749..2639
                     /locus_tag="AC1ZCC_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HIQ1630939.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          945 a          419 c          519 g          841 t
ORIGIN      
        1 ttttaaaaca tcacaaggaa aaacttaact atggtattat ctaaacataa ttttttcggt
       61 atttaatatt taattaggag gtaatatttt gcttttttat agtaatttta tcttaatagt
      121 agcgatatta ttacttctga atatatggat ttttgatagg agtagaaatt caagtatagg
      181 ttttagaact aaacgaagtt tgtcttctaa aaaaaattgg gtatactcac aaactatttt
      241 ttatggagga attgttttaa ttagtttact ttcttcaaca ctttattctt tgaatattat
      301 agatgtttcc acctcaaatt caatatctat tattggaatc attattgctg caattattac
      361 acaactgttt ttagtgtttg gagagaagaa aagaagtaaa aaatagaaaa actagtgata
      421 gttttggtga tataagaaaa acatatatgt ataaaaagaa gaaagtagct tctctttgta
      481 catatatggt ttgtttttat ggatgccgag cttttttata agataaggct accgcccata
      541 acgacccaag gatttgtgga actaagtaaa tacctgcaag aagcaagatc agctgggtcg
      601 gttttaaagg atgatagaag taacgccatt cagtaaaatt ggcgcaaaat aaataggaag
      661 tgacagtaga tataatattg gcaatgaata aagatataat acgcctgttc ctttgaaaat
      721 aatatccagc aaaaagagat aaaaatatca atgtgatgaa cccgcttacg gaatgataac
      781 gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa agataaaagc aacggaaggt
      841 ttttccagca agtttttatc atctttcatc accacctaac aagtgttatt attttgttag
      901 ttttattgta aattgaatcc tgcaataatc aattactttt tcattatttc tagattaaat
      961 gaataaactc tcatgccatt atctctaaaa aaaaagtttt tccccctaat ttttttagat
     1021 agaaaattgt atagtatgat gagagaaaaa gtagtttggg ggaacgttaa tgagaagaaa
     1081 agcagtaatc gatcggcttt atttattttt ttggtttttt cctatcgttt tgctaccttt
     1141 tacatgggta aatgctgttg tacaaggtgc ggagatatta ggcctgatcc aaggaagcgg
     1201 atttgtggtt tctaggaatt ttccagcttt gattgttttc tatttagtga taagtgtagg
     1261 tctcttatta ccaaaatctc tgatcaatca ctacaattgg cagctgcttt acttttcttg
     1321 cttattagta tttacatcta cgttttcttt gctgcctcgc ttgattatcg gccctaatgg
     1381 tttgagcgag agtggattga aggatttagc ttattcctcc atgtatttct tgacgatcaa
     1441 accatggttc tatttagtaa ttgcctcatt tattcttgca acattttttt acttctggac
     1501 agaaatcagg ataaaaagtg aacgatctgt atctataaaa aataactatt gatggtggat
     1561 catcagtaaa aaaacatatg agctgcgtaa aatgcatgtt tctcatatgc tttttttatt
     1621 attatgtaaa gaatgcgaaa aacgtttaca tgatttttac ataacttaac tatattttta
     1681 cagcaaccat acgagggatt tacagtcgac tgataaactt tagttgtaaa taagaaagga
     1741 gcaataaaat gaaaaaagta ttgtttactc taggtatgat gggaattctt ttagccggat
     1801 gcggctcagg atcaggaaat acagatggct ctgccacaca aaataattca agtgactcaa
     1861 atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt acagccgtta gtcgatgcag
     1921 cacaagaaag ctttgtacaa gaaaatccaa attatcaaat ctctgtacaa ggaggaggaa
     1981 gcggaactgg tttgagccaa gtcgaagctg gagcagttac tatcggaaac tctgatgtat
     2041 ttgcagaaga aagagacgga gttgatgctt caaaattagt ggatcataaa gtagcagtgg
     2101 tcgggatggc accaatcgtc aataaagata cagatgtcaa agatatcacc aaacaggaat
     2161 tgattgatat ctttacagga aaaatcacta actggaagga agttggcggg aaagatcaaa
     2221 aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg cgcgacattc gaaaaatggg
     2281 gactggatgg tgctacccct gtgcagtccc aagaacaaga ttcatcagga acagttcggc
     2341 agctggtaag tcaaacacca ggagcaatca gctatttagc tttctcatat ctcgatgatt
     2401 ccacacaagc actaagtatc gacggtgtag aaccaaaaga agagaatgta gcagataaca
     2461 gctggggaat ctggtcatat gaacatatgt acacgaacgg aaaaccttcc cctgaagtcc
     2521 aaaaattctt agactacatg atgacagaag aaatccaaga aggacctgtc aaagaattgg
     2581 gctatttacc aatcacaatg atggaagtag aacgtgatca cgaaggaaat atcaaataac
     2641 tagcagataa acatgccacg gtagtcgtta ctattaagga aaaatcaact atgtttcccc
     2701 acatatacat ttttccttta attg
//
LOCUS       DBNICI010000223         2655 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.230, whole genome shotgun sequence.
ACCESSION   DBNICI010000223 DBNICI010000000
VERSION     DBNICI010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.230"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            848..1522
                     /locus_tag="AC1ZCC_002733"
     CDS             848..1522
                     /locus_tag="AC1ZCC_002733"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIQ1630940.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            1519..1860
                     /locus_tag="AC1ZCC_002734"
     CDS             1519..1860
                     /locus_tag="AC1ZCC_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630941.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            1890..2249
                     /locus_tag="AC1ZCC_002735"
     CDS             1890..2249
                     /locus_tag="AC1ZCC_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HIQ1630942.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
BASE COUNT          902 a          445 c          570 g          738 t
ORIGIN      
        1 agctaatatc gagggtcagc tgattgaaca ccgtttatat caagaggttg tgcgcctgcc
       61 gttcagctcc gagtcacaag gagtaatttc aaaaaacagt ttttcatgtt tgatgactat
      121 tgtgacttgt gaccgaggag ttggccggag agcaacgttt atttaaaaaa aagaactgcg
      181 aactctcgca gttctttttt taaaatacgg gataaaggaa gtttccagca gacaagagaa
      241 gaaagcaaaa gaaattgcta tttgattcac aaggttctat aacgaaatcg caatcaatca
      301 aacatataca aagaaggtta ttcaaatgga aatctgattt gatgatgctt ctgctgttga
      361 aataaagcag catctagtac ctgaaaacaa actaatagta acattcgaag atggagtagg
      421 tccgtatttt cagcatgcaa tgatccatat gtaagtttaa ttcatccttg aaatcatcgg
      481 accagataaa gttatgacca atctatccca agcaatctag aagagatatt agtaaaagaa
      541 catttactgg aagtcttaga tgagcctata aatatatgtt ctgatggcgt catcagtggt
      601 ctgattgacg gaagcttagg tgtgatcgat ttctctggac caaacaacga aggaatcaag
      661 gggaatccgg cacgttaaga aaaaaagtat aaaaaactag acacttgatg agcgttcatt
      721 caattgattg aagggactag gctggtgtca ttttttatac atatattttc tctttcgtct
      781 tgatagggct tatgggataa tataaggaag aaaatcatcg ttttattcaa aaaggagttt
      841 ttgacaaatg aaaaaacgta tattatttat cggtgacagc attacagacg caggaagaga
      901 ccgagcagaa gcaactgatc taggtaaagg ttatccactt ttgattgccg gaaaactaat
      961 ctcccaatat ccaacgatga aatttgaatg catgaaccgt gggataggag gagacaaaat
     1021 tgcagatctg caagtacgct ggcaagaaga ttgtttagct ttacaacctg actatgtatc
     1081 gattttgatc ggaatcaatg atacctggca taatactagc gatcaggcta tattcggaac
     1141 tgagaaatct gcgcagcaat ttgaatccgt ttatcgaaaa ttactaaatg aacttcaaac
     1201 agcggaaatc tgcaacatca tcttgatgga gccgtttgta cttcctataa ccgacgaccg
     1261 tcatggctgg cgtaaagact tagaccctaa aatccaaatt gttcgtaaac ttgctaagga
     1321 atatggcgca ttgctggttc ctttagatgg catattcaat gctttaggga ttgcttatgg
     1381 tttttcgaaa tatacaagag aagatggggt ccacccaacg attggcggac atgagatcat
     1441 tgcacaaagc tggatagatg caattgccgc agtcgatttt tttagtggag ataaaaatga
     1501 gcgaaagaaa agtgagaaat gatggaaata cggtgggcag taaaagaaga tttacctcaa
     1561 atcaatcagc tgattactga tcatccagaa tcattcgtaa aagcaaaaac ggcagctgac
     1621 ctaaatgata agaataaact attgctcgtt gctgtaacaa aagaaaaacg gattatcgga
     1681 tttgcgcaag cggacgtgca acagtcagaa gaacgggtgg atcagcgacc atcaggaaaa
     1741 ctaatccaac acacattagt aacaaaagaa aatacggaag aaatccaaaa aaaatttgca
     1801 gaatacttga tgctggaagc taaaaaaaga agaataaaat caatttttgc ccagttttaa
     1861 tcaaaaacag taatagaagg agaaatacaa tgagttgttt aggaaatgtc atctggttta
     1921 tattcggcgg actaatcggt ggtcttagct ggttagcagc ggggatactt tggtgtatca
     1981 cgatcatagg tatcccaatc ggtatgcaat gttttaagat cgcctccctt tcttttgcgc
     2041 cttttggcaa agaagtaatt tatcaccagt ctggcatgtc attgatcgtt aatatcatct
     2101 ggctgatcgt ttcaggccta ccaattgctc tcgcacatgt cgtcagcggc atccttctat
     2161 gtatcacgat catagggata ccttttgcca agcaatcttt caaacttgct cgattggcgt
     2221 tgatgccatt cggtgcagaa attgtataaa aagagaataa taaaggttag aaaacgcata
     2281 gagtcaggtg ggatacatgt atttcacctg actctatgtg ttttcctgta taagttctcc
     2341 catgtgactt ttacgtcttt tatttatatg ggattactgt ttatggaaag ttgaaataat
     2401 tttcattaaa gaactctttc tatatttttg aaagcgtagt ggagcttcga atatctggga
     2461 aggacatttt gcagggattg aaaaaagata ttgtcctatc gaaaaattaa tgaatttaga
     2521 caaattgcca gcaacaggct tcaaacttag ttgctatcct gtcaagatca agaaggcaag
     2581 tgcgggttgg ataagagcag gggcaatgat cgaagagaaa aagaaagaat aagccaaaac
     2641 atctgtatgg aagaa
//
LOCUS       DBNICI010000224         2640 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.231, whole genome shotgun sequence.
ACCESSION   DBNICI010000224 DBNICI010000000
VERSION     DBNICI010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.231"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(131..697)
                     /locus_tag="AC1ZCC_002736"
     CDS             complement(131..697)
                     /locus_tag="AC1ZCC_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIQ1630943.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(697..1653)
                     /locus_tag="AC1ZCC_002737"
     CDS             complement(697..1653)
                     /locus_tag="AC1ZCC_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HIQ1630944.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1653..2309)
                     /locus_tag="AC1ZCC_002738"
     CDS             complement(1653..2309)
                     /locus_tag="AC1ZCC_002738"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIQ1630945.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT          824 a          533 c          484 g          799 t
ORIGIN      
        1 ttggttggac ttattaccgc agatataagc ttttgagcac cgtgaataaa gaggttatga
       61 aatcaacgct ctaacctgag tattagagaa aaaaacgcaa aacatatcat ttatgttttg
      121 cgttttttat ttatcttttt ttctcaatac aatataaaat aggcggctgg ttcttttggt
      181 tgataaaccg ataattcagc acattatatg tttcctgtgg cagtttggaa caaaaagaat
      241 caaccatatc cagttctttt tctccacctt catgaccata gtagacgaca aggatcaatc
      301 gtccgcgagg cacaagtcgt ttcaatattt cttccatagc tgtgcgtgta gtctctggca
      361 tagtgatgat tgctttatca ctttttggga gataccctag attgaaaatc cctgctttga
      421 gattttgttg ttcagctatc acttctccta acgtttcgtg gccttgtaga aacaatgaga
      481 cacgttcttc taactgacgc tgttctagcc tttcacgagt agcatcgatt gcttgttgct
      541 ggatatcaaa actatagaca tgaccagttt ttccgatgtg ttctgctaga aaaaccgtat
      601 cgtggccatt gcccatcgtt gcatctacga cgtgatcccc tggttgcaag atttcttgca
      661 gtaatgtatg gctgaaatgc aaagcagttt gcaacatcag acgctcacct ttttccgtac
      721 atcatatttt ccttggaagc tatctctgcg aagaagttct tcatcaattg cattcaagac
      781 ttcccatttt ttcaagctcc acattggtcc aatcaaggaa tcccacggcg catcccctgt
      841 caaacgatgg atgatgatct cttttggtat catttccagc tggtcgcaaa tgacattgac
      901 atagtcagga cggctcatta attgaaggcg accttcatga tagtcccgca acattctcgt
      961 tttgcgcatc agatgcatca aatggagctt gattccttgg atatctgaat ctaaaatggt
     1021 gcgtctgaca ttttctttca tcatttctaa actttcgcct ggtaatccgt tgatcaaatg
     1081 cgtacaaaca cggatattat gtttgcgtaa tttagccacg ccatctaaat aagtttgata
     1141 atcatgtgct cggttgatca gttttgaagt ttcttcaaaa gttgtttgca atccaagttc
     1201 tacccataaa tacatccgct cgttcagttc agcaaggtaa tcaactactt catctggcag
     1261 acaatccggt cgtgtaccga ctgataatcc tacgacacct ggtaaattga ctacttgctc
     1321 aaatcgttct ttgattattt caactggcgc atgggtgttt gtaaaatttt ggaaataaac
     1381 gatgtattgt tggacatgag gccatttttg atgcatcatg tcgatttctt tctggaattg
     1441 gattggaaga gggtcttcag gtgcaacgat catgtctccc gaaccagata cactgcaaaa
     1501 agtacaaccg ccatgggcaa cagtcccatc tcgattcgga cagtcaaatc ctccatcgat
     1561 cggcaccttg aaaattttcc cgccaaactc atttcgtaaa gcgtaattcc aagaatgata
     1621 tcttttattt ccttctgaat aaggaaaagt cattaaaagt ccttctttct gtaagagtta
     1681 tctatttgtt ttcttttttc tctatacact ggataagtca ggcacagcca agaaagactg
     1741 agtaagtaac cgccgatgat atcagtagga aaatgaacgc ccagataaat gcggcttatc
     1801 ccgacactta ctataataaa catcatcagc aattgaagca atgtgcgtaa ggttcttttt
     1861 tgaacaaaag ctggaagcag aaatagtaaa gaaccgtaaa gaatcatact gcctgctgca
     1921 tgtccactag ggaaactgta acttgtttca actactaagt gcgccaatga aggacgctct
     1981 cgcatgaaaa atagtttgat caccgggttg atgattccag caattacaat aaatccagaa
     2041 acgaaccaaa atgtttctgc cctttttttc ttataccaca tccataaagc cgcacaagca
     2101 aataaaatga tgacggtaag aggattaccg aatttagtca taaagagttg gtagctgttc
     2161 agatcaggat aagaagctct gacaatgcct gtaaaccact gatcgaaacc agaaagccac
     2221 gacagatgga actttacgat ccagcccaaa atgaaaaaca ggacaagaaa gaagctacca
     2281 gccacctgaa atgatgtttt atatttcata caattcctcc tttgaagcaa tgacttatta
     2341 tagcattttt ggtccagcga aaaaaacggt aaagagataa aaccaatgta tgattcctct
     2401 ttgaataacc aatattctta taaaaaaaat aactgtaatc ggaaagacat tttttggtaa
     2461 tgcaaatgaa atgttaggga aataaaatca gtgtttttcg aaatcctttt aaacaagaac
     2521 gtagacgtag gaagaacttg aaaatgaaca gaagttgttc attacggtaa attacaatat
     2581 gattacaaaa aacaacaaag ctcttaaata acttttaata aatattaaga gaagttataa
//
LOCUS       DBNICI010000225         2628 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.232, whole genome shotgun sequence.
ACCESSION   DBNICI010000225 DBNICI010000000
VERSION     DBNICI010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.232"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..227
                     /locus_tag="AC1ZCC_002739"
     CDS             <1..227
                     /locus_tag="AC1ZCC_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIQ1630946.1"
                     /translation="KTTEFDILHALMSTPNKVFSRSDLIEKVKGIEFDGLDRSIDSHI
                     KNLRHKIENDAKSPMYILTVHGTGYRFGGH"
     gene            227..1620
                     /locus_tag="AC1ZCC_002740"
                     /pseudo
     CDS             227..1620
                     /locus_tag="AC1ZCC_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294791.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1635..1841
                     /locus_tag="AC1ZCC_002741"
     CDS             1635..1841
                     /locus_tag="AC1ZCC_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630947.1"
                     /translation="MFQKQIKIAKLVCECWAKKIFFQLISIDSVEKVHLDILSNSVVI
                     FSKKDLSIATIKKEVFGLQIYEMT"
     gene            1862..2458
                     /locus_tag="AC1ZCC_002742"
     CDS             1862..2458
                     /locus_tag="AC1ZCC_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HIQ1630948.1"
                     /translation="MKTVKKMTKLVFASLGIIVFLGACSNQSESNNSNSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
BASE COUNT          967 a          353 c          536 g          772 t
ORIGIN      
        1 ttaaaacaac tgaatttgat attctacacg ctctaatgtc cacacctaat aaagtctttt
       61 ctcgtagtga tttgatagag aaagttaagg gaatagaatt tgatggacta gataggagca
      121 tcgattctca tataaaaaac ttaagacata aaattgagaa tgatgctaaa tcacctatgt
      181 atattttgac agtccatggt acaggataca gatttggtgg ccactaatga gaaaaagaac
      241 gataaaaact caattagctg tttccttttt agcaatcgca acattaataa taggatcgat
      301 tagtttagtc gcattaagtt tgatgaacaa tcattttagt aaatacgtgg aagagcggca
      361 agaagatttg ttgaatcagt atgtatatac aattgattta ttgtggttaa atagtgggga
      421 aacatggaat agtgaagaac ttgcagcact atctgagaaa gtattagaga ataatattta
      481 tttttctata gaagacgaac aaggcaacat ggtttgggaa cttactggaa aagatttgaa
      541 gtcagcacag gagaaactga aaaaaatgca ttgaaagttt ctgagaaaaa cagtgtgaaa
      601 ctagacgaaa caatagaagt gaaaaagaag ttaattaatg atggaaacga gtttggaaaa
      661 gtaacgtttt attatttcgg accttttgct tatacagagc acgatgcctt gtttatctcc
      721 agtatgaaac aaagtttaat gtatgttgca attgcagctc ttcttgtatc attcatactt
      781 gcttcgtgga tctctgctag attaggattg ccactaaagc atgtaagcga ttttacacat
      841 aaattaactc gtggggaata tgctgataaa atcccacaag aaacttcaat tattgaaata
      901 aattccttaa ttgattccct aaatgattta agtaatcagt tggagaaaca gcatgggctt
      961 cgaaaaagat taactaccga tatttcacat gaattaagaa ctccattagc tacgcttaaa
     1021 ggcaatgtgg aaggaatgat tgacggagtc tggaaaataa ctcccgagcg cctacaatct
     1081 tgttatgacg aaatagatcg tctaactcgt ttaattggaa atatagaaat aatcaacaaa
     1141 attgaagcaa aatatgatca tttaaataaa actgagttta acatatataa gttgattgaa
     1201 tccgttattg aaaatttcgc cagcaaaatt gagtcaaaaa accttcatgt tgaaatccaa
     1261 ggggataaca tcaatatatc tgcagataaa gataaaatga accaagttgt tatcaatctt
     1321 ttgaataatg ctattaaatt tacgcaaaag gagggaacca ttaagttttc tataagtaaa
     1381 aataaagacc atgttctttt aattgttgaa gacaacggca tcggtataga aaaagatcag
     1441 caacttcata tttttgatag attttatatg gctgacccct ccagaagtag agctttaggg
     1501 gggcaaggta tcggattagc tattgtaaag agcgttattg aagcgcataa aggtagtata
     1561 actgtaaaaa gtaaattagg tctaggaact aaatttgtta taaagttacc atttcaataa
     1621 aggaggagag ttatttgttt caaaaacaga taaaaattgc taaactagta tgtgaatgtt
     1681 gggcaaaaaa aatatttttt caactgattt cgatagatag tgtcgaaaaa gttcatttag
     1741 atatcttatc aaattctgta gtaatctttt ctaagaagga cttatcaata gctacaatta
     1801 aaaaagaagt ttttggatta caaatttatg agatgaccta aatgtgatta attaaaggag
     1861 gatgaagaca gtgaaaaaga tgacaaaatt agtatttgca agtcttggaa ttattgtatt
     1921 tttaggagcg tgtagcaatc aaagtgaatc gaataattct aatagcacaa acgaggaatc
     1981 gacttctaca gcgtctagtg aaatgaatag tatggaggga atgaatcacg aaggtatggt
     2041 tccttcttca atgaaagatg ctgcgaaccc taaattcccg gtgggaagta atgttatact
     2101 gctaggcgat catatggaag ggatgaaagg tgcaaatgct caagttgtag gggcctttga
     2161 tactacaatg tatgaagtaa gctatgagcc aaaaactggg ggaccaatgg ttaacaatca
     2221 tcgatgggta gtacaagaag agttaaaaga tactgagact gtagcaaatg agggcgatac
     2281 tgttatcttg aatgccgatc acatggatgg gatgatggga gctgaagcaa aagtagataa
     2341 atctattaca ggaactgttt acgttgttaa ttacactcca accgatggtc aagaagaagt
     2401 taagaatcat atgtgggtaa ctgaagatga gatggaatat gacgaaaata atgagtaatt
     2461 ttatatacta aaccaacaag ctggtacatg gtcttcgtat tctcaacatg aggaatatga
     2521 gctctttgtt aataaggact gatgagttgt gcaaaatcaa atctgggtca gaacgaacct
     2581 cattctggct cagatttttg gctcttcgac atttagtgct ggtagaga
//
LOCUS       DBNICI010000226         2559 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.233, whole genome shotgun sequence.
ACCESSION   DBNICI010000226 DBNICI010000000
VERSION     DBNICI010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.233"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            544..1107
                     /locus_tag="AC1ZCC_002743"
     CDS             544..1107
                     /locus_tag="AC1ZCC_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIQ1630949.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1386..1673)
                     /locus_tag="AC1ZCC_002744"
     CDS             complement(1386..1673)
                     /locus_tag="AC1ZCC_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIQ1630950.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(1663..1992)
                     /locus_tag="AC1ZCC_002745"
     CDS             complement(1663..1992)
                     /locus_tag="AC1ZCC_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIQ1630951.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2297..>2559
                     /locus_tag="AC1ZCC_002746"
     CDS             2297..>2559
                     /locus_tag="AC1ZCC_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIQ1630952.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSR"
BASE COUNT          875 a          389 c          397 g          898 t
ORIGIN      
        1 ttaaaacttt tttagcagat tttaaagttt cgtcaaaact aatatattct ggtagctgag
       61 cattcaaata gctttcagac ttcagaagaa aattacgagc atttttatag tccatctcta
      121 ataaagttat tcttttttta tccaatacat tcagctcaat tccttataat attctattcc
      181 ttagtatctc aacattattt actttataaa acgaataaat tttgtagttc taattcaaat
      241 attgcatgtt atatctaata tgggagcatt ttataatttt cattgtgata aactcgttat
      301 taactttagg atacactagt taggattaaa ttaggtatct agaaatttac ttaagatttt
      361 tttgtttatt tcgtatacac actcgtatca aaaacaatcg attatgagac aaaatgaaat
      421 gacctctttt attaaataaa agaggtcata ttttgtatca taacttgttg cattaactat
      481 gtatctataa ctccctttta tgatacaatc aagccagaat taaagtacga aaggaagaaa
      541 agcatgagtg gatacattta tggttatgcc cgagtgagta cacgttcaca agaactaaat
      601 cgtcaattag acttactcaa tgaacaaaat tgtaatgaaa tcctaactga gaaaatgacg
      661 ggaaccaaag cggttcgtcc ccaactaaat cgtttaaaag ataaaattcg tccgggagat
      721 acggtaattg tagaaagttt ttctcgattg ggtcgtagta caaaggattt gattgacctt
      781 gttacctatt ttgaagaaca ccatgttaag ctggtgagct tgaaagaaaa cttcgatacc
      841 agcacgcctc acggacgctt aatgatgact gtcttccaag catttagtca gttcgaacgt
      901 gatttaattg ttgaacgtac caaagaaggt ttgaaaagtg cacgtgcgag aggtcgtaaa
      961 ggtggccgtc cacgagtgaa ccagaaagat gttgatcgag cagtaaaatt gtataaaagt
     1021 caggtttata gcgtaaaaga aattacggaa atgactggta ttagcaaggc aacactctac
     1081 cgctacttaa aagacaatag ggaataatct attttaatct ttaggaatct ataatattta
     1141 tgggagaatt agagtgattt ttttattatt actaaaaaac aatataaaat aacgcatttt
     1201 aagccctctt tgtgcataag gatgaagctt ttaatttgaa agaggcttaa aatgcgtttt
     1261 aattttttct atttttcata aatatatagg ttagtggttt aaaattatag ggtattacaa
     1321 ctctcagctg ttaaaaatta tcttttaaag tgtcgtctat cctctcaagt tgaaacattg
     1381 ggttcttacc taatcttttt taattgttca taaaaatttt catgcgttcc acatagtaag
     1441 ataggtatca ctgcattatc tttaatttca tatgcaacgc gatatgtcgt acctgcatac
     1501 ttgaatccaa tagcccaaat tcctgataaa tcacccgttt tttgttctcc cgaatgagga
     1561 cgaactgcta tctcatcata aattatcgtt aaaaattgtt cttttaatgt tttctctttt
     1621 aattttttta gatactttat tacgtgttta gaaggtagaa tctcataagc caatttctgc
     1681 tgccaactcc tcttttgtca tcggtttaga atttgcaaca gtatcttcag caatagattt
     1741 aaatgcagaa gttaacgcat tttttctatt tctaaattct tttaagatct cttgtttgct
     1801 ataaccttct gaaagaatat cagccaaaat atcttcacta aaatcaaaaa attctttttt
     1861 aggctctaca aactcataaa ggataccatt ctctacaagc ttagcttcta cttcaacacc
     1921 attgggcaca tcgaattctt tagggaccgt taacataata gaatttcctt gttttcttgt
     1981 ttttgtaatc atattatttc ctccattatt ttacatttcg taattactta gtaattacat
     2041 tgtacgctat ttttagtaga aatacaaaat aataaaactc ctttagaaca attttcaaag
     2101 gagttttatt tgattaggaa attatgtaga ataataatat gtttttaact tggaatcaaa
     2161 aacgcacttg caagtaagtt tacttcaata catgaaattg cttataatag tgtattggaa
     2221 ttaaggtaga ttgacaaaag agtgatcaaa taggtatact tcattcaaat aatcgtttga
     2281 atgtgaggtg agatgaatga aaaaagtttg ccaagtgact gtcgtacata aagaaaaagt
     2341 aaatggaata aaaaataaat tggatcaaaa agatttttca aacttacttg tgttagggaa
     2401 atgtttcagc gatttttcta gaattaaaat tttctatgct ttagaaacct ataaggaaat
     2461 gtgcgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
     2521 acgtttttta aaaaaacatg gaatggcaaa atcacgtca
//
LOCUS       DBNICI010000227         2407 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.234, whole genome shotgun sequence.
ACCESSION   DBNICI010000227 DBNICI010000000
VERSION     DBNICI010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.234"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..1022)
                     /locus_tag="AC1ZCC_002747"
     CDS             complement(<1..1022)
                     /locus_tag="AC1ZCC_002747"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HIQ1630953.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNT"
     gene            1539..2354
                     /locus_tag="AC1ZCC_002748"
     CDS             1539..2354
                     /locus_tag="AC1ZCC_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1630954.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
BASE COUNT          802 a          406 c          388 g          811 t
ORIGIN      
        1 tttgtattat ctgttatagg gatcttcatc tggtgtgaaa aacctggtct cgtttctaag
       61 atcgacgtgc caatgtataa atggacacaa cctgttttac agttatgttg cctgatacga
      121 atagctactt gttccgccat ttcttttacc accagttcga tttcatttct cctggtataa
      181 tctcgtggta acacttgtga attaccatat gatttttcct ttgtcggttc tggtgggatc
      241 gctatatctg ttcgatcgat cccgttcgca tgaaaataca gctgtaagcc aatcacacct
      301 aaatggctct taatagtgta tggattccaa ttagctaagt ctttaatact catgatacca
      361 atttggttga gtcttttttt cattcgagat ccaatacccc aaaggtctgt catttcagga
      421 atattccata ctttatcagg aacattttga tacgtccatt cagcaaggaa tccatcatta
      481 tgttttgctt cattgtctaa agcaagttta gctaataagg gattatctcc gacacctact
      541 gtagcaagca atcctagctc ttctttaata ttctcctgga tcatttgagc cagcttcttt
      601 ctccgttgac ttcttgttcc ttcagtcgta aaaagattca ttgatctggt cacttttaag
      661 atcgattcat cgatcgagta aatcaataaa tcttcgtctg ctacatacct tctgaaaata
      721 ttgtttacct gcatatttcg cttaatatag agattcatgc gtggtggaac gacatgtaac
      781 gttttaggaa aaggttgcgg tagatctcgt ggtcgactga cattagaaat accgtacttc
      841 ttctttgcag ctggtgaaga agctaaaatc aaccctgatc ctgtattatc cgctcgactc
      901 atgacgacta attcagtcgt taatggatct aaattccttt ctatacattc gacacttgcg
      961 taaaagcttt tcatatcgat caaaaagtaa tcatttactg gctcttttga ataatccatc
     1021 ataaataaca ctccattctt ccacattata caaacatacg ttcgtttttg taaagaagaa
     1081 tcgattaaat gtacaaaaaa agaagcatag gtaagttatt tccctacttc ttctgttgat
     1141 ttgtaaaatc aacacattgt atcactttat taaatggata tttactatta gtcttgtgaa
     1201 aatttgaaaa taagtgtcgt attatccagt tttgcttagt tatattagaa aaaaataaga
     1261 aattcaaaaa agaaaaaact ttccttaaaa acaaaaaata ggagtattca gtttcttaat
     1321 agttttgtaa tagttgtatg tagaagtttt cttaatagtt tttgtaataa tttattaatc
     1381 gtagtttata taacttattt gtaagtgact agagaattaa ataaaaaata attactatct
     1441 agtacgaaat taattacata ggataatata ttaaggtgct ggactacaag taaaaaatac
     1501 tgtatgatca tgacagattc aagaaaggag caataagatt ggttaagata gcaggacttt
     1561 ctaaaggtgg aaaaaccgtt tcacaacttt ataaacaaaa ctacagagac tggagacagt
     1621 tcagaaaaaa cattaaggat tcttacttta ttctaccaac agaacttgaa aaatatttac
     1681 cgaataacaa agctattaat ttatatctgt attactgttt taaggcaaag aatgaaggcg
     1741 gggattcctg gcattcggtc aataccatag cagagtctct aggagtgaca actaaaacta
     1801 taaacacctg gaataaaacc ttagttgatt taggtatgat tgcacgtata gacgataacc
     1861 atctcagtaa atcgacaatt ttacttcctc tctctagtta ctataaatta gcccttgata
     1921 gttctttaaa ggatgttacc gagagcactg tccatgaaat tgatggcaac ttggttgcag
     1981 tttatcattt gtttgaatgg agaaaaaatg aggctgatga aaattataac gttccttata
     2041 acacactctg ttgtgttttt aaaagaaaaa cacaaaaaga tactatctat aaatttatct
     2101 tggtccaaga tgagaaaata agtaatttcg aattaagcac tccttctagt aagatttatg
     2161 acgatgcata tagatttgaa tgtcaagatc tagaagccct cctttccgca aataatctaa
     2221 acaatataga acatgaaaac tttgttgtaa acactagatt taatttagtt tctgaaaagg
     2281 actcagattt gttagatttg ctgatagagc ttactaaaaa tctagataag aaagaaaaca
     2341 ttactgtact ataactagca tcggtccatc attaagtttg gactattaga acacttgcta
     2401 ttacaga
//
LOCUS       DBNICI010000228         2398 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.235, whole genome shotgun sequence.
ACCESSION   DBNICI010000228 DBNICI010000000
VERSION     DBNICI010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.235"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            207..2069
                     /locus_tag="AC1ZCC_002749"
     CDS             207..2069
                     /locus_tag="AC1ZCC_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HIQ1630955.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="AC1ZCC_002750"
     CDS             2069..>2398
                     /locus_tag="AC1ZCC_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HIQ1630956.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DBNICI010000229         2375 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.236, whole genome shotgun sequence.
ACCESSION   DBNICI010000229 DBNICI010000000
VERSION     DBNICI010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.236"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(4..249)
                     /locus_tag="AC1ZCC_002751"
     CDS             complement(4..249)
                     /locus_tag="AC1ZCC_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630957.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(266..1117)
                     /locus_tag="AC1ZCC_002752"
     CDS             complement(266..1117)
                     /locus_tag="AC1ZCC_002752"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIQ1630958.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            1283..2287
                     /locus_tag="AC1ZCC_002753"
     CDS             1283..2287
                     /locus_tag="AC1ZCC_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HIQ1630959.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT          756 a          402 c          478 g          739 t
ORIGIN      
        1 tttctatttt ttcggtatca aggcaaaaac aggtagttca ggtgaataaa tgacttcatg
       61 ttcgtgccag ggtttgacca ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat
      121 ttgatcatgt gtccagtttc ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa
      181 gacaaaatcg gaatgaaaag tctgggtaac ttcttccgag agagaattca agcgttcgat
      241 tggatccata aaatcacctt tcgacttaaa attgggtagt atctgggtct tttgctttgc
      301 cgactacacc atctaactga tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa
      361 ataattcagt attttctttg attctgcttg gtgtcactga cttaggaaga ggaaggaatt
      421 cgtgttgcag cgaccaacgc aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga
      481 tttgtttcat ttcaggcaca tcgaagattt taccagtgcc taatggacta tatgcttcga
      541 ggatcatatc atgttttttt gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa
      601 ggaaaacttg attgaccatc ggcataattt ttgctgtttt tgctagtgta tctagatggt
      661 ggggtaagaa attgctcaca ccgattgctt tgattttacc tgcttcatac agttcttcaa
      721 atgctcgcca tgtatctgca tttgcttgtt cccaattatc acgaaaagca acagggtttg
      781 gccagtggat caaaaacaga tctaaataat ctgtttggag cttcttcaat gattcttcaa
      841 aagaagacat cacaagttca tagctgtggt ttgcattcca taattttgtt gtcaagaata
      901 tctcttctct aggaattcca ctttccttaa tcgcttggcc gacgctttct tcattcttgt
      961 atccttgggc agtatcgata tggcgataac ctgcagccag cgcttcttta acagaggaaa
     1021 cagcgacatc cccatcaggt gtttgccatg tgccgaatcc tactaccggg atttcgtaac
     1081 catttgatag acgataagtg cttgttaatg aattcatact ttcagctcct tttttattcg
     1141 ttcaactata agtgtagcgc atttttcgaa aaaaagaatc cattttgttt ctactcttga
     1201 aatttttgtt taaaagaaaa tgattttgat tctggtattt ttgatttttt ttccgtaaac
     1261 taagacagaa gggaatgaga aaatggatat ctatttaaaa aaagcaattc tgcatattat
     1321 tgacagagaa actggttctc cagtcttttc acaaaaagag ctggatctga caaaagaata
     1381 tattcgtgat tttcttcaaa agaagatcca aaaaatctct tctgctcaaa caaaaactgg
     1441 acaactagca gaagaaagca tgatcgttga atcacttaaa atcgctcaag aaaattttgt
     1501 ggaagcaagt gaagcaatcg ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc
     1561 acctagtgca gatgtatttg ttgtcttgta tgaattagat acagtgatgt acttatcttt
     1621 attgaaagtc aattatcgtg aggcttacac tcactttgtt gaagcagacg aagcagggat
     1681 cgacaaccgc ttgattttga atcgagcaat tctaggggcg aaaacacaaa aagcagatga
     1741 agcattcgct gtgaatttga ctgattggac ctacgaatta attgaaaaaa aatatgagtt
     1801 ttctggcaaa aaagagcttt atttttctgg aagagtgatc gaaagtgttc ctgcaccttc
     1861 tttagaagaa aatgtaaaag tgatccaaaa agtagccaag catcttggaa aaaaatttga
     1921 gacagaagaa tttgatatca tggctgattt gaaagaagct gtctatgata cgatagaaga
     1981 aaaaggtcgg ttggatcatg agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc
     2041 aaaaatggct tttaaagaag aagtacaaga gcaaggattt gtcccagaag cacctcctgt
     2101 aaaagaagta caagaaattt ctgagaaaaa atttggaaaa caaaaattca agctatcaaa
     2161 cgggatcgaa ctgatcgtac cagtagatgt atatcgcaac ccagatttga ttgaatttgt
     2221 gaataatccg gatggtacga tatccgtgat gatcaaaaat gtggatgaag tattgaatcg
     2281 tttataaaaa tgactagaat attaggtgtt caagtaccag tgatcagtta aatgaattgt
     2341 gtcaagagaa ggatcggaaa agacgaatat aacaa
//
LOCUS       DBNICI010000230         2333 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.237, whole genome shotgun sequence.
ACCESSION   DBNICI010000230 DBNICI010000000
VERSION     DBNICI010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.237"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(365..1657)
                     /locus_tag="AC1ZCC_002754"
     CDS             complement(365..1657)
                     /locus_tag="AC1ZCC_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HIQ1630960.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(1931..2191)
                     /locus_tag="AC1ZCC_002755"
     CDS             complement(1931..2191)
                     /locus_tag="AC1ZCC_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1630961.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          779 a          440 c          352 g          762 t
ORIGIN      
        1 tatgggctac tctgtgatac ttattagcca ttaaactatt ttacccacat agcatatatt
       61 ttgaaatcgt tcattccgta aattcaacta aatatgccta aaaaaataaa aaatctaaga
      121 tagaataaga tttattcagt cttagatgga tttatagcaa ctttttcgta acaactttaa
      181 aaaatacgaa aagaaagagc tgttttactc tgactacgtt gctaaattgt cgtttaatca
      241 ttcatttaaa ccaaatcttt ttttatagaa ttcgttttaa tcctcctgaa tccaaaaact
      301 atgaaaaccg taattaaata gttaagagtt tttaaccacc ataattctct cggctatttt
      361 gaaatcagat aaattttgat cggacacata gttgaataaa cctaccgttg ttacggaagg
      421 taaatcgcat acttttcatt ctagaggaga aggtctcgtt gatcattaac tgattaagca
      481 ccatagccca gttttgaatt cgaccacctg accacttcgc gtgtaattct ttcgtgcgta
      541 aataaagtag ttttaggagt gcattctcat tagagaacgc tccttttttt gtgacttttc
      601 tgaagctgga gtggacactt tcaaccgcat tggtagtgta cataattttt cgaatggcac
      661 taccataatc aaatagttgt tcaacatgtg caaagttccg tttccagaca tctacagcac
      721 cagaataatg agaccaccga ttttgaaaac tgtcaaaagc agcatgtgca gcgtttagag
      781 aagaagcacc gtagaacttt ttcatatctc ggcagacttc cttatagtcc ttacttggaa
      841 tatagcgcaa tgcatttcga acaagatgaa caatacagcg ctgaacaatt accgatggaa
      901 agatcgcttt tgcgccttct tcgaggccag aaacaccgtc catcgaaatg aaaaagacat
      961 cttcgacacc acgtgctttc agttcatcaa atacttgcat ccagcgattt ttagattctg
     1021 tttgatttaa ccataatcct aaaatctcct tatttccttt gagatcatag ccaagaatgg
     1081 tgtatacagc atattctttg gcttcataat tttctcgtaa agtaacatac atacaatcaa
     1141 cgaatagaaa ggcataacac tttgctaggg ggcgggcttg ccattcttcc aattcaggaa
     1201 ggacagcgtc agtgatatct gaaatcattt catgggaaat atcaaagcca taaatagctt
     1261 cgacggttgc ggcaatatct cgttgactca ttcctcgtgc atacatggaa agaaccttcc
     1321 cttcgatgtc ggagacatct cgttttctct taggaattaa ctctggttca aaggaagctt
     1381 cccggtctct aggaacatca atagctactt caccaaaact ggttttaagc gttttagttc
     1441 catagccatt tcgacggtta tcgtgttcct taggctcttt agaatgggca tcataaccta
     1501 aatgattatt caattctcct tgaagcattt tttcaaaaag gggcccaaac acatctttca
     1561 aagcatcttg catgtcatcg acagattcag gttgataggc attcagaatg gattcagcta
     1621 acttttcggc atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatctta
     1681 ttgtagaaaa agaaaaaccg cgaagcaacc acctgcctag gattaatggt tacttacacg
     1741 gtttatatta cactctcatc attaaaaaag tatctttcga tattactaaa caatgaaatt
     1801 atctatttga aatttaaatc tgataatata ttgagtgttc caatgagggc tttgatccta
     1861 ttatccatat ctttttcagt tttaatattg atacacatta taagaaaaag aaacttttgg
     1921 ttaatttatt tcatcccagt gcaaaatata aaaaagtaaa atagaccata ggcaatagat
     1981 taacaattaa tataatacaa gcaaatattc tattcattgt atttctgctt tcaaaaagtt
     2041 ctaatattga aagtataatt ccacaaatgc ctataataag tatgggaata agattagaaa
     2101 ataacacccc gagccaatct gtgaaaatta ctatatttac taatatccct attaacatac
     2161 ctatcatatt tattaaatca atttttttca ttagatattt ctcctttatt aaatctgaag
     2221 tacttatatt aaaataattc taaactattt acctcaaaaa gtgcaataaa ttatttctga
     2281 acaataaaat gatagtcaaa acaaaaaaat attgttaatc gttcagagcc atc
//
LOCUS       DBNICI010000231         2304 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.238, whole genome shotgun sequence.
ACCESSION   DBNICI010000231 DBNICI010000000
VERSION     DBNICI010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.238"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(146..718)
                     /locus_tag="AC1ZCC_002756"
     CDS             complement(146..718)
                     /locus_tag="AC1ZCC_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIQ1630962.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(734..1339)
                     /gene="fic"
                     /locus_tag="AC1ZCC_002757"
     CDS             complement(734..1339)
                     /gene="fic"
                     /locus_tag="AC1ZCC_002757"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HIQ1630963.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            complement(1740..>2304)
                     /locus_tag="AC1ZCC_002758"
     CDS             complement(1740..>2304)
                     /locus_tag="AC1ZCC_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1630964.1"
                     /translation="YFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEY
                     EVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRA
                     LGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVG
                     VSSVIGLVFSVMPASEAAKKDLIDILR"
BASE COUNT          688 a          498 c          318 g          800 t
ORIGIN      
        1 attactttac cttttacagt taccatacac attttttgag cagaaatcaa gtaaataaaa
       61 aaatattata tttcacaaca tgctaataga ctcatatcta gaacgcatta agtatttacc
      121 tgtctggttc gctgagactt cttttttaac tttcttcaat tttcttcttt attctaaaaa
      181 gcgtactgcg actgaaaccg gttatagctt caacttcctt atacgattta cctgaagtaa
      241 gcagttcata tgcgtgcctc ttcttcggtg tgattgttgc tttcggacgt ccttctctga
      301 aattaggatt attctttctt gcaaacattt ttccttcttg ggtacgttcc acaatcatat
      361 cacgttccat ttctgctaca ctcaacaacg tccgtgtcac cattcgcccc atggacgtat
      421 tttcaatcgt tcctagattc aaaaccttga tcgttacgtt atcatcgagt aaaggttcaa
      481 tgatctctaa tgcttctctc gtgttacgag caaatcgatc aagcttcgta acaactattt
      541 catctccagg cttcagacca gccaacagct gattgaattt tggccgatcc cgttttgtac
      601 ccgtaaattt ttctgaaaaa atattcttct tctctacgcc gttctcaagc aactgttgaa
      661 tttgttcttt tgtactttga gaagcggttg agacacgtgc atatccatat ttcgtcattt
      721 ggtccaccta cttttacaaa tcatctatcg tatactcaga atacccttca tagaaatagc
      781 tgacatcgat tcctttcata tataactcgc gatcgtcaat tttatcagtc agagcattgg
      841 aaattagata gcgtatttcc aaatcgttta tgggacttcg ctccattgca gaaagatagt
      901 ctgctttatc gattaagttc caatctacaa ctttttgcaa ttcttccttt aaaatgagat
      961 ccagccaaat tctagtactt cggccatttc cttctcgaaa tgggtgagcg atattcatct
     1021 ccacatattt cttgataatc tcttcaaaag aagtttgtgg catctgatca atatgctgta
     1081 acgaatgttc taaatacata actggtgcaa atcgaaaatt tccttttgca atattgaccg
     1141 atcggatttt tccggcaaaa tcgtaaatat ccgaaaatag aaattcatga atttcagata
     1201 aacctttaaa tgtaccaact tcgatttcat ttatttttcc acaatcgtac aattctttcg
     1261 ctttttgttt actgagtttt tcttcaacct tagccaactc aacttgattg gttaatccca
     1321 atttattctc taaaatcatg cgaaatctcc ttaactttat gacataagtt tatgaaacac
     1381 tctcaacccc attatatcgc gattttgttt atgtgtcaaa aactattaag tttctgaccc
     1441 ccgatagatt tttcaagtgt ctctattttg cacagtctag ttgacctttg atacacctct
     1501 atatttatag agaatctaaa acatcaacat ggcatactca aaattctaaa tattagggat
     1561 aaagaattct cctatacaat caaaaatttc ctttatttcg aactccggtc acctaactct
     1621 attgatctct tacaagccaa agaatatctc tatactacaa agaaacaata gggtgcctgg
     1681 ttggtctctt tgtgttttat tttagaatct agaacgtaaa tattcttgtc attctgattt
     1741 tatctaagaa tatcaattaa atcttttttt gcagcctctg aagcgggcat tacagaaaag
     1801 accagtccaa taaccgaaga taccccaacc gctagtaaaa tagtaaatag atcaagtgac
     1861 acgtttacct ttatcaaaga accaacacta taagcaagag ccattcctaa taaatagcca
     1921 attaaccctc ctgaaagagt taaaatcaaa ccttctaaaa ggaactgcag cataatcgat
     1981 tttcgagtag ctcctagggc tcttcttata ccgatctctt ttgttcgttc agatacagat
     2041 atatacatca tgttcattac acctacccct gcgataaata aagatatacc tgctacagct
     2101 gttataaaat aagtgatcgt actgaggatt tgaccaattc ctttcgttaa catagccgta
     2161 tctaacactt catattcccc caaatgtttc aaactacctt tttctttcaa ttggtttatc
     2221 actttagagg tcaccttgtc aggttcaact ccttcctcta aagttaacgt tagagacgat
     2281 gtatcctttt cagacttaaa ataa
//
LOCUS       DBNICI010000232         2297 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.239, whole genome shotgun sequence.
ACCESSION   DBNICI010000232 DBNICI010000000
VERSION     DBNICI010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.239"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            37..222
                     /locus_tag="AC1ZCC_002759"
     CDS             37..222
                     /locus_tag="AC1ZCC_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HIQ1630965.1"
                     /translation="MNYKWILCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIK
                     QFKVTVITEPDAKTQSR"
     gene            complement(282..635)
                     /locus_tag="AC1ZCC_002760"
     CDS             complement(282..635)
                     /locus_tag="AC1ZCC_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1630966.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            1140..1448
                     /locus_tag="AC1ZCC_002761"
                     /pseudo
     CDS             1140..1448
                     /locus_tag="AC1ZCC_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            1424..1771
                     /gene="mef(H)"
                     /locus_tag="AC1ZCC_002762"
                     /gene_synonym="mefH"
                     /pseudo
     CDS             1424..1771
                     /gene="mef(H)"
                     /locus_tag="AC1ZCC_002762"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
     gene            1998..>2297
                     /locus_tag="AC1ZCC_002763"
     CDS             1998..>2297
                     /locus_tag="AC1ZCC_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HIQ1630967.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRY"
BASE COUNT          751 a          350 c          435 g          761 t
ORIGIN      
        1 gatttgaatt aaagtgtaaa ggaggagata gttaaaataa actacaagtg gatattgtgt
       61 cctgtatgtg gaaataaaac acgattaaag ataagggaag atactgaatt aaaaaaattc
      121 cccctctatt gtccgaaatg cagacaagaa aatttaattg aaataaagca gttcaaagta
      181 actgtgatta cagagccaga cgcaaagacg cagagccgat aaaatgagat taatacaatc
      241 tcattttatc ggctctttcc gttatgtatg gattctttta attagtcttc gatgtttctt
      301 gcttcgttga taccgctggc taaagattcc attaaggata gttctttgtc tgtaaagcta
      361 tccatgtatt tctctatctg taatcgtcgg gtgcttttta ccaagttatt agcaggtaag
      421 aaaaattcat caacggaaac atgaagtaac gatacaaggt cataaagaac ttgtatgctg
      481 gggtgttgcc ctttattttc aatattagtt aagtaccgtg ggtcaatttc aatcaatgct
      541 cccacttgtt cacgagttaa acctcgtttc aatcgagctt ctttaatggc taaaccaaag
      601 gctctaaaat catatttatc ttctttttta cgcatagtag accacctcta tacattttat
      661 tgttcctact gaattaaaaa caggtataga aaaacgtgtt atatggttta taggtttata
      721 tttaataaaa agcactacta aacgccaata aaaaaaaccg ttatatggta gtgctattta
      781 cgctgttaaa atattgtata ttacttccaa atggcggttt gttggaggtc aacgtcgcca
      841 tgaagtacat catatacaat aaatttcctt acattgggtt cttgtcaaaa aaagtcgtct
      901 atctgcaata gataagtacg tccaccaatg tggttttata aatcatatag atagaataac
      961 agaagcatgt aaacagagaa ataaatctgt ttatatgctt ttttggctat tcagaacttt
     1021 tttacaaagt ttatttatca gtaatgcaac aaatccccct ttcacattgg gactaagagt
     1081 gaaaggagat aaacgagcaa ggctcacttc ctttcctaga cagaaagggg gtgagaaaca
     1141 tgaaaccatc ttcttttcag accacaatag aaaatcagtt tgactatatc tgtaaacgtg
     1201 ctatggaaga cgagcgaaag aattatatgc tttatctttc aaggattgca aagcgtgagg
     1261 tgtccttttc ggatgttggc gattatcttg ttagccagtt tgcgacaaca gataactatt
     1321 caactgactt tcagattttt acactcaatg ggttatcagt aggcgttgaa aatgatttgt
     1381 tgagtgaagc attacgtgag ttgccagaca agaaacgtga aattctactg ctgttttact
     1441 tatgggaatt tcgctatttt tagaattgcc tgcaatagtt tcctactggg tagttacagt
     1501 gatgtgcttt gtggctatgg gtgtatctac tatgtttgtt gttcaaattt acactcttgt
     1561 tcagacacaa acaccaccgc aattagtagg aaagataatg gctacgctgg tagcagttgc
     1621 tatgtgcggt caacctattg gacaaatggt ttacggtatt ttattcgatg tatttataca
     1681 agaaacatgg gttgttatgt ttgtagcttc tattgcttca atcatgattt caatatattc
     1741 aaaaagaatt tttcaaaagt tagagatata atacaagggg ttaaatagta tgttaggcta
     1801 aaaacaatat ataaaagaaa atttgaaatc tgctaaaagg atagtgttat aaaagaaaga
     1861 gtaatattgc agtatttaag gctcgcacaa atatatgttt tgtgtgggct tttttcttac
     1921 catttttctg tatttctaaa gggtataaac ggtattccgt ttatatattt cagcattagc
     1981 agaaaggggg tgagattatg aggacatctt ctttcgagca tatcgtcaga ttacagttca
     2041 attctttaat gctgattgtc attaagaaca aagtgaaaag tcgaaacaga cagcttgcaa
     2101 gacattctaa acgtgaagta ttgttttgtg aaatagccga aacgaaacaa gttgaacgtg
     2161 gaacgattga tacctgtttt tatgattgtg tatcatttaa agtcttgcac tttacaattt
     2221 atgtatctga tgaaacactt ggtacagttt tacatagact gtcagaaaaa cagcgtagtg
     2281 cgattttatt acgttat
//
LOCUS       DBNICI010000233         2216 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.241, whole genome shotgun sequence.
ACCESSION   DBNICI010000233 DBNICI010000000
VERSION     DBNICI010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.241"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            91..1935
                     /locus_tag="AC1ZCC_002764"
     CDS             91..1935
                     /locus_tag="AC1ZCC_002764"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HIQ1630968.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            1991..>2216
                     /locus_tag="AC1ZCC_002765"
     CDS             1991..>2216
                     /locus_tag="AC1ZCC_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HIQ1630969.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPK"
BASE COUNT          676 a          371 c          483 g          686 t
ORIGIN      
        1 aatttattta gtttctgcct gttttttgtg ttgaggatgg ccacttcgac tcaaaagttc
       61 atcactcaaa aaagaaaaga gggaaacacc atgaattaca aacaaacagc agaagaagta
      121 ttgcagtaca tcggtgggga agaaaacata tcccatttgg agcattgctc aactcgttta
      181 cgttttacgt taaaagacaa cagcaaggta aatatgagcc agttagaaaa agtggatggc
      241 gttatgggcg ttcgccaaaa tgtgcaatgc caagtaatca ttggaaacga tgttgttgaa
      301 gtgtatgatg aagtgaaagc gttattaggt gacaatgttt caaatactga agaagcacca
      361 aaacaaaaac aaaaattagg ttcagttcta ttagatttta ttatttctat tttccaacct
      421 ttaattcctg cgattgcagg tggaggagta ttgaagtcct tgttgctact agcttctatc
      481 ttagggctga ttagtgacca atctcaaacc tatcagattt taaatttggt cggcggggca
      541 ccgctttatt tcttgcctat tctggtggca atgactacgg ctaataaatt gaaagtcaat
      601 caattggttg cagtatctgc tgtcggtgca ttgatccttc ctgaaatgac agctatgttg
      661 gcggaaggtg ctacattctt atcttttgga ttggataata tagcgtatgc atctcaagta
      721 ttcccagcca tcttaactgt tttattttat gctcaacttg aaaaatggtt tacaaaagtt
      781 tcaccaaaac caattcgtat tttctttgta cctatgatga gcttattgat tacagttcct
      841 attgctttgt tgcttctagg accattaggt tataatgtag gaacagcttt ctcttctatc
      901 attgtgtctt tgtacaatca atttggatgg gttgcaaccg gagtcttagc agctgtactg
      961 ccattaatgg ttgtaacggg tatgcataaa gcaatgatcc cttacgctgt ttcatccatg
     1021 agtgaattgg gtgcagaact tctttatctc ccggcttcat tggcacacaa tatttcagaa
     1081 gctggcgcat gctttgctgt cagtatcaaa acaaaagaca aaaaattgaa atctacggct
     1141 gtttctgcgg gtatatctgc attattcgga attactgaac cagctcttta cggggtaacg
     1201 attctgcaca aacgtgtttt atatgccgtt atgttttcta gcctgatcgg gggagcattt
     1261 gcaggaatcg ttgcacttca agcctttgct ttagtgggtc ctgggattgc aagtatcacg
     1321 atgttcgctg acaaagctga cccaatgaat ctggtttggg catttgcagt atcggggatt
     1381 tcatttgtgg ttgcttttgc tgcagcgttg gttctttata aagataaaga agttgtcaat
     1441 gaagttgaaa ctaagatgga aacggccgtt gtttcagatg ctgttctttc aagtcccgtg
     1501 aatggaaaag taatctcact atcagaagta aaagacactg ttttttctag tggtatggta
     1561 ggtgatggag tagcaatcat tcctgaagaa gggatattag tagctcctga agatggagaa
     1621 atcacaatgg tatttgaaac gaagcatgct attggtttaa aaacggctaa tggtgcagaa
     1681 cttctttttc atattggtat tgatactgtt cagttagaag gaaaagggtt tgaggcattt
     1741 gttcgttctg gtgaaaaagt agccaaagga caaaagctga ttgcatttga tattgattta
     1801 atcaaagaag caggattcga ccccacagtt atctgtgtgg taaccaataa ggataattac
     1861 actatccgtt catttaacct agaaaaattg gtaaccaatg atagtgatct cttagctatc
     1921 agtccgatag cttaaacaca agatagtgaa aaaaaatata ttgaatgaag gaatagagga
     1981 gaatagaata atgacaaaca caacaagttt tccaaaagga tttttatggg gcggcgcgat
     2041 agcagcaaat caagtagaag gtgcttggaa tgtagacggt aaaggtcttt cagtagcgga
     2101 tatggctact tataaaccaa atttagacaa caaagatttt gctgctcata tgcatgtcac
     2161 cctgcagtct ataaaagaag ctagtgaaga caaatctgat aaatggtacc caaaaa
//
LOCUS       DBNICI010000234         2179 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.242, whole genome shotgun sequence.
ACCESSION   DBNICI010000234 DBNICI010000000
VERSION     DBNICI010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.242"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(43..1362)
                     /locus_tag="AC1ZCC_002766"
     CDS             complement(43..1362)
                     /locus_tag="AC1ZCC_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIQ1630970.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1359..2012)
                     /locus_tag="AC1ZCC_002767"
     CDS             complement(1359..2012)
                     /locus_tag="AC1ZCC_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIQ1630971.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          721 a          332 c          177 g          949 t
ORIGIN      
        1 aagtctctat ttttcaataa ctattgcaaa attatatctt aattaagaaa atatttttat
       61 tttaaagata aaaaattctt catcctgcaa tactttatat tctatattgt aattattcag
      121 aatattactt acaatatata aacctaatcc attactattt tccttattta aatcaaattt
      181 aacatcaaat attttatcca tattcgaaat tttattgtta ccgtatgaat tctctatata
      241 taaccaatca ttaactatcc caatattaat taccccattt acatcagtat attttaccgc
      301 attactaatc aaattagata gaataatctt taaagctgtt tttcctatat aaacattctc
      361 gtctaatata taattattga cagttatttt cttttgattt gctaatattt catacttttt
      421 taatatgtct tctaatgtat catttatttt caaatattct tcatcattat tcaaattttc
      481 tatagaatga actgataata tttgagaaat attttttgtt aaactatcaa cgatttcaat
      541 acattcatta atataaatat ctctttcttt atattttcca atattgtatt tcatgttttc
      601 cagtattatt ttaagactag caagaggggt tttaagttca tgggatgcac ctttaaaaaa
      661 gtcatatttt aacttttcta attttaaaat ttctttattt ttaaattcca aatcgtcaat
      721 tgttcttaat aaagtagaat acaaatcatt aatttgttgt tttaattctc caacttcatc
      781 attagagcta acttttaaac tcattttttt atcaagtttc atcattttat cagtaacaat
      841 ttttatttct tgtatattat tttttattga ttttgcataa attaaagaaa taatagcaga
      901 aaataaaatt gatattaata aagaatatgg taaaaattta aggcttaaat ctttcgcatc
      961 tttttgcata tcagctgtag aaacaaattg tagatttgtt tttttaccat cattaagctt
     1021 tatttctctt tcttcaatta tcaaagaatt actatcactt tctaaattaa catttatatt
     1081 atctttgatt tgtatttcat tcttattatt tttttctttt atgaatactt ttatttcact
     1141 actcttggaa taaaactcca aagtttgttc tatatatttt atttcttttc cattcatatt
     1201 actagaaatt tcatttgcca tattatgaat cttttctttt ctcgtctcca aatatgtttt
     1261 aggaaagata aaaaaaatta atgaatgaac taatataatt attattccaa gaacagaaaa
     1321 tatttgtatg aacatttttg gaaatatctt taattttttc attttctctc caatttatat
     1381 cctacatttc ttatagtagt tatacagtct aattgtaatt tttttctaag ttcttttata
     1441 tatacatcta ttactcgatc ataaggagtt tcttcgctat ctttccatac ataatcaata
     1501 atttgcattc ttgttaacac ttgtccatta ttatccaata aacattttaa tacttctagt
     1561 tcttttgcat ttacatctat ttcttcacca tttattttag ctgtatagct attaaaatta
     1621 accgatagat ccttatattc aaacttctct aaatgtccat aattcttctt aattaaagaa
     1681 tctattctag cttttaagac cggcaatgaa aatggctttt ctacatatcc gtctactaaa
     1741 ttagtaaatg catcaatttt atattcttca tcactaaatg cagtcaagat tagaattggt
     1801 aaattgcttt tctttctaat ttctttcaac acttctaaac catttataaa aggtatctga
     1861 atgtctaaga taaccaaatt tatatcgcta ttaaattttg acaaggcttc tcttccatct
     1921 ttagcttgaa taacagtata tccaaattct gaaagatatt cacttattcc ctctcttatc
     1981 atattatcat cttcaacagt taatattttc atatatacct ccacttaagc ctatacacta
     2041 gatattatac agcatattat ggctctataa tatctagtgt tggtgacgcc ttctgttgcc
     2101 aatacttttt ctttttttta ataaatgcaa acagacacct tgaaatcctt tagaaaaaag
     2161 gcttctataa tattttgtg
//
LOCUS       DBNICI010000235         2110 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.243, whole genome shotgun sequence.
ACCESSION   DBNICI010000235 DBNICI010000000
VERSION     DBNICI010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.243"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..605
                     /locus_tag="AC1ZCC_002768"
     CDS             <1..605
                     /locus_tag="AC1ZCC_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIQ1630972.1"
                     /translation="PKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVD
                     KKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEA
                     GTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQS
                     SKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            739..2070
                     /locus_tag="AC1ZCC_002769"
     CDS             739..2070
                     /locus_tag="AC1ZCC_002769"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIQ1630973.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFIFSFVKLS"
BASE COUNT          819 a          265 c          382 g          644 t
ORIGIN      
        1 taccaaaagg aactacttat acttggaaag aaaaagtaga tgttagtaca gctggaaaca
       61 aaaaaggtac tgtagtagta acatatccag atggatctaa agaagaagta gaagttacta
      121 tttctgtagt agataaaaaa gcaccgaata aacctcaagt tgatcctatt acagagggtg
      181 accaaattgt tactggtaaa actgaaccaa atgcagaggt gacagttaca ttaccagacg
      241 gaagtcaaca ccatgggaca gctgataaaa atggtaattt tacagttaaa gttcctaaat
      301 tagaggcagg gacaaaagtg atagtaactg caactgatga atctggtaat actagtgaac
      361 ctactaatgt agttgtttct tccaacaaaa aagacagtgg aaaaaacggt agtaaaggta
      421 gtaagactga caatcaaggt agcaacagca atcaagacaa aaatagaggc aaaagccagt
      481 cctcaaaagt ttttcctaag acgggagaga gtgacagtaa tatctttact attagcggtg
      541 gtttaatact gttaggaacg ttaggattat tagggtataa aaatcgaaaa aaagagaatg
      601 aatagaaaat caatttctta agtcttctct gagaagagga agaatctttt tcttcctctt
      661 tttgcttttt tatgatggga aataagtgtt agttaaagat gaatatactt tttatagaaa
      721 ggaaaagaat gtaattaagt ggaaaaaata gtaattattg gttcaaacca ttcagggcta
      781 gccgcagctc gatttctaaa cttaagcgat tcaaattatg aagttattct aatcgataaa
      841 aaatcagaaa ttagttactt ggaatgtggg acatcgttat ttataaaagg agaagtaagt
      901 aatttagaag atctttttta tcaaaataga atgacactaa attctgagat aaaaaagtta
      961 tttctagaga cagaagttga caaaattgat attcaaaata aaaagattag ttgtgtaaaa
     1021 aaagatggta caagattctc tcaagaatat gacaaagtta tattagctac aggttcaaag
     1081 caaattatgc tagatatacc ggggaataat ttagctaata ttttttcgat aaagaatttt
     1141 ccaaatgcaa aagagatttt taaagctttg gatgatagta ctattaaaaa agtaggaatt
     1201 attggagcag gatatattgg agttgaaatt gctgaagcca taagaaaaag agggaaagaa
     1261 gtatatcttt tcgatgttgc agatagagtg ttgtcgactt attacgaccg tagtttttca
     1321 gataaagttg aagaaatact atcaaaaaat ggaatacatc tttgtctatc agaaagtcct
     1381 aaaaaatatt taggaagaaa aaagattgaa aaaatagtca ctacaagtgg tacccaatac
     1441 tctatggata tggtcgtaca agctatagga tttctatcta atagtccaat tggggaaaag
     1501 gaactcttga gagatgttag tggagcatat ctaactaatg aataccagca aacaaatata
     1561 aaagatatat atgctattgg ggattgtgct acaacttttt ttacgtccat taatagaagt
     1621 agtgttgatt tttctatttc taatgctcta agaacagctt atattgcaac tcaacatatt
     1681 aacaatcaag attttacacc ttctggttcc caatttacaa atggcttttc tatatttaat
     1741 tacaattttt acgcagcagg tttgaattta aaaacagcac gattattaag tgagtctata
     1801 gattatatag aaatagaaga tttcattaga cctgcttttt tgagaaaaaa tagtcgtgta
     1861 aaattaagaa ttagctttga aaaaaataca aagagaatag taggtgtaca actttgtagt
     1921 caagaagatt tatctggact attatcaatg ttttctcttg ctatagaaga acaaattaca
     1981 ttagacaaat tgaaaatgtt agattattta tttcatccta gttttagcca accctataat
     2041 tttatttttt cgtttgtcaa attaagttag acaaatcatc ttttgtgacg taactcaaaa
     2101 atacttccat
//
LOCUS       DBNICI010000236         2056 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.244, whole genome shotgun sequence.
ACCESSION   DBNICI010000236 DBNICI010000000
VERSION     DBNICI010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.244"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            1..305
                     /locus_tag="AC1ZCC_002770"
                     /pseudo
     CDS             1..305
                     /locus_tag="AC1ZCC_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            389..1558
                     /locus_tag="AC1ZCC_002771"
     CDS             389..1558
                     /locus_tag="AC1ZCC_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HIQ1630974.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
     gene            complement(1898..>2056)
                     /locus_tag="AC1ZCC_002772"
     CDS             complement(1898..>2056)
                     /locus_tag="AC1ZCC_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HIQ1630975.1"
                     /translation="RRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEI
                     KVLLGIPA"
BASE COUNT          736 a          319 c          348 g          653 t
ORIGIN      
        1 caagatatat taaattgatt tttgaatttt tgaaaaaaga aagaaatgct aatgcagtta
       61 ttttagaccc tcataaaaat aatccaagag caataagggc ataccaaaaa tctggtttta
      121 gaattattga agatttgcca gaacatgaat tacacgaggg caaaaaagaa gattgttatt
      181 taatggaata tagatatgat gataatgcca caaatgttaa ggcaatgaaa tatttaattg
      241 agcattactt tgataatttc aaagtagata gtattgaaat aatcgatagc gtaaaatatt
      301 ttgtgtaaat agaaatttag ggtagaaaaa aaagtccatt ctgtaaaatt aaagttacca
      361 cacagaaatt ttataggagg actttctcat gactaaattt actatagaaa ttatgcaaac
      421 actactcaat aaaggggatt tagatgaatt atttcaggat catttagagc gagcgattaa
      481 ttcacttcta caagcagagc taactgcttt tttagattac gaaaaatatg atcgaaatgg
      541 attcaattca ggaaattccc gtaacgggaa ttattcacgt acatttaaaa cagaatatgg
      601 cgagttgaat ttaactattc ctagagatcg gaatggcgaa ttttctcaac aaacactacc
      661 agcatataag cgaaccaatg attcattaga aacgaccatt atccaattat ttcaaaaagg
      721 cattactatg gctgaaatct ccaaattaat tgaaaaaatg tatggtcacc actatacgcc
      781 acaaacaatc tccaacatga gtaaattggt ggctgaagat gttttagctt ttaaagaaag
      841 aacgttagaa gccaattatt ccgttatatt tatggatgcg acgcatatcc ctgtgaagcg
      901 acagactgtt tcgaaggaag cagtgtatat tacgattggt attcgtttgg atggaaccaa
      961 agaagttctt gggtttacta ttgccccaac tgagtctgct tatatttgga aagaagttct
     1021 tcaagatctt agaaaacgtg ggttagaaga agttttatta gtagtgacag acggattaag
     1081 ctgtattgaa gaaagtatcc atagtgtgta tccgaatgcc caatttcaac aatgttgtgt
     1141 gcatgtatct agaaatatcg ctcataaagt tcgtgttcga ggtcgaaaag aaatttgtga
     1201 ggatttcaaa ttggtttacc aagcgaattc aaaagaagag gcattggatc acatcgactt
     1261 tatgactagg aaatggaaaa agcagtatcc aagagtcgtc aatttactct tgaatcctgc
     1321 cctattaacc ttttataatt tccctcacgc catcagacga acaatttatt cgacgaacct
     1381 gattgaaggc tttaataagc agctaaaacg atatactcga agaaaagaac aattccctaa
     1441 tgaagaatct ctagagagat tcttcatttc tcaatttaat caatataacc aaaaattttt
     1501 aggtagaatt cacaagggat ttaaagaaat tcaggataca ttagagtcga tgatttaact
     1561 gtaattaaca gaatggattt tccatttaca tataattctt gacgctacca taatctaaat
     1621 aaccatcata ttcatctact acttatataa ttgagtttta ttaaactttt taagtttcta
     1681 ttctatcaaa tatttaaaca atctctgcat ccgaaaagac tacctcgtat agttttacta
     1741 acaatatttt tattagcgtg ttcaaatcct gatttcttgt atcaattatt ttctgttcac
     1801 tttcaataag aaaaaagtct ttacttccat ggataataat aagggttctg ttgcaaagtt
     1861 ttaaataaag aataaaatcc cttacggtat ctatgattta agctgggatt cccaataata
     1921 ccttgatttc agtacagacc gaaaacccga agagagtgcc ttcttttcgg gttttcttat
     1981 ataatcctcg gatggcttcc atgcctttaa tcgttgtaga ggcagtgcgt aaacttcgat
     2041 agaatttatt gcgtct
//
LOCUS       DBNICI010000237         2041 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.246, whole genome shotgun sequence.
ACCESSION   DBNICI010000237 DBNICI010000000
VERSION     DBNICI010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.246"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(332..817)
                     /gene="vanZ-A"
                     /locus_tag="AC1ZCC_002773"
     CDS             complement(332..817)
                     /gene="vanZ-A"
                     /locus_tag="AC1ZCC_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HIQ1630976.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(970..1881)
                     /locus_tag="AC1ZCC_002774"
     CDS             complement(970..1881)
                     /locus_tag="AC1ZCC_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HIQ1630977.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT          639 a          392 c          287 g          723 t
ORIGIN      
        1 tctttagcga atgaagacat cctttccgct attcgtgttt tttaaagtta tcagaaaatc
       61 tatctatgaa atcttagttt gggggtaccg tcaggaaaat gcggatttac aacgttaagg
      121 aaatttatta aagagaccaa ttcctcagca ctaaaattga taattgggct ttctttgtta
      181 ctatagtgtt aggtaagctt tttactttat caccggtttt cccctcactt cacacctacg
      241 ggcgagtttc cccacatccc gttttccatc taatccaatt cattcaatat ctttatgtgt
      301 atgaaagatc agattgcttg atttagacat cttacacgta atttattctt aaatgggtac
      361 ggtaaacgag caataatacg agcaaaagta tacctacaaa aataataact ctgtctaatt
      421 ttttttgatt catatgctta ttgcttaaac catataattt cagtccaaga aagcctccaa
      481 cagtatttgt aattacatct gttatgtctg tcgctccaat agcgaagata aattgaatta
      541 tttcaaaagt aagacttaaa accagtacaa aagcaaactt aggtaaaaat ccgatttctt
      601 taaaattgac attcaaaagc aagccaaatg gaataaagat tataacatta tctatcatct
      661 ctctgaaatt cccagtagca gtaaatggag tcaagttaag acttctttga tgataattaa
      721 atactgataa aatattgtat tgtaatttga ataacactaa ccagattagt gtcactaaat
      781 ataaagctag caatcctcta gataatattt ttcccaatat aatttcctct aattcttttg
      841 tttaagttgt atcattagta tagcaatgtc ctagttaacg tgggttttaa attaaaaagc
      901 taacgttacc ccaaaagtgc tagtacaata atgctgttca ttaatttcgt tggtttcatt
      961 ccgccatcct tacctccttg aattagtatg tgttgatccg ggaaacactg tcacaattac
     1021 accgtctata ttgtttcctg atatctcata acgaagatta gtcggcacat gaatggtggt
     1081 atttttagta acaggataat aaaagatctc atatttttcc ccatttacac taacagaaat
     1141 ggttttttct tcttttaggt aatccatata ttcctcgaga acgaaattct tttctttcat
     1201 aatcgcacta tgtggtaaac caacatagcg aatatgccat ggttcatatt gaattcctgt
     1261 taactctgtt ttgtcctctg gataacgtaa aatgaacccg tatttccaag cattttcttc
     1321 tatccacttt ccttcagggg ctcgttccat tttcgtcaag cttgatccta catctagtga
     1381 taaacctgaa ttatgctcac tataacctgc tggtaaggca tactcagccc ccatttcttg
     1441 gtaaagcaca ctttgctcat caaagtctcg atagccacta ttaataataa aatgactaac
     1501 gccacccttt acagcatcat tgaccatctc tgaaaatttt tgtgctattt cttttgacat
     1561 ataaatatta ctatcaagca acccgtatcc atttattaat tcgtcatgtt tagataaatt
     1621 cacgatatct gacttcacac tttcttggcg aacaggatat ttactattga ttaatagcag
     1681 atttccttga taaacctgtt cttctgtaat tgttttctct tgggtatttt cagaagtccc
     1741 actattttct aaatgttctt tgggattttg atcataattt tgaaattcga ctttttcctg
     1801 agaaaacagt gcttcattaa cgtagtcata acctaagtat attaagaata acaataacaa
     1861 taaaaaaaac aacttcttca ttttcagtct cctccttaac tatatagttg gaagtatgaa
     1921 atatttctag aatcttcccc ttaaacggca caatctcgtt tgagattgtg ccgttttgcc
     1981 ccgtaaatta cagtcaatta tttcgcccta ctggatttac tattatcacc aatgtagatg
     2041 a
//
LOCUS       DBNICI010000238         2024 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.248, whole genome shotgun sequence.
ACCESSION   DBNICI010000238 DBNICI010000000
VERSION     DBNICI010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.248"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(53..1393)
                     /gene="glnA"
                     /locus_tag="AC1ZCC_002775"
     CDS             complement(53..1393)
                     /gene="glnA"
                     /locus_tag="AC1ZCC_002775"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIQ1630978.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1421..1813)
                     /locus_tag="AC1ZCC_002776"
     CDS             complement(1421..1813)
                     /locus_tag="AC1ZCC_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIQ1630979.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          574 a          449 c          341 g          660 t
ORIGIN      
        1 gtttctcgtc aaaacgctga tttcacagcc tctaattcac ggtattcctg ttttaataaa
       61 gttctagata ttgttctctt tcccactcgg aaacagtttg tctgaatgcc gcccattcga
      121 gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca atcattacat
      181 tatccttacg caattctttg atagcattat ggatagttga aggtaagtcc tggatatgtg
      241 cagcttctct ttcttcctca tccattacat aaatgttacg gtcaacagct gctggcggtg
      301 tgatctgatt acggataccg tctagtcctg cttgcaacaa tactgccatt gccaagtatg
      361 ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg cgagattctg
      421 gtacacgtac taatggcgaa cgattacggc cactccatgc gacataaaca ggcgcttcat
      481 aacctggaac gagacgttta taagaattga ctgttggatt gcaaacggct gtgtaggcac
      541 gagcatgttt cagcaaacca cctaagaaat gataagctgt ttcacttagt tccaattcac
      601 cgtctttatc atagaaaaca tttccttcat cattgaacaa agacatattg caatgcatac
      661 cagaaccatt gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt ccatgcttgc
      721 gtgcaatcgt tttgacaacg agcttgaagg tttggatatt atcacaagca tctaccacat
      781 ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt gaagcttcca
      841 cttcaaagcc tagactttct aattccaaca cgatgtcacg gcggcaattt tcacctaaat
      901 ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg atcccaccgt
      961 cttcgtctaa tttaaataag aagaattctg gttcaggtcc taaattgaaa gaggtaaaac
     1021 caaggtcgcg catatctttc aatgctcgtt tcaaattccc gcgaggatcg ccggcaaatg
     1081 gtgtcccatc cggattatag atatcacaaa ttaaacgagc aacttttcca tgagcacttt
     1141 cccacgggaa gatcatccaa gtggagagat ctggatacaa atacatatcg ctttcttcaa
     1201 ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat tttattttct aatactttgt
     1261 cgatctggct cactggaact tctacatttt tgatcgtacc taagatgtct gtgaacatca
     1321 atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt gatttcttca cctgttatct
     1381 gtttctttac catggtatcc ctcttccttt tttaatttat tcagtcaaag aattgtcttt
     1441 gcaatccttg cgactgataa tgacctgttt tgcttaaccc accttgcgcc aaaatctcat
     1501 cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt gcttcttgtt
     1561 ttttcgcctg tgccatttcg tatacacgtt tgatcccagc catattcaat ccctctgaaa
     1621 gatagtcttt aatctcaagc aacatatcaa tatcattcag cgaatacata cgtcggtttc
     1681 cttcgcttcg ttccggatga actaaatctt gttcttcata atagcgaatt tgccgagcag
     1741 acaaatcagt cagcttcata actgtaccaa ttggaaatac ggacatagat cggcgcaatt
     1801 ccttttctcc catcagacgt cccctttcgt tttataagtt ggctaaagtg taccaattaa
     1861 aaaaaatatt gtcaactgca tatgttaggt tttctaacat gagatctcta ttttttataa
     1921 aataacaaaa aaataagaac acaaaaaagg tatctagttg attttcgtac cagatacctt
     1981 ttatttttta ttatactgta ctatccttta ttagtgtttt tatt
//
LOCUS       DBNICI010000239         2003 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.249, whole genome shotgun sequence.
ACCESSION   DBNICI010000239 DBNICI010000000
VERSION     DBNICI010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.249"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..378
                     /locus_tag="AC1ZCC_002777"
     CDS             <1..378
                     /locus_tag="AC1ZCC_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIQ1630980.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="AC1ZCC_002778"
     CDS             426..1826
                     /locus_tag="AC1ZCC_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HIQ1630981.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DBNICI010000240         2003 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.250, whole genome shotgun sequence.
ACCESSION   DBNICI010000240 DBNICI010000000
VERSION     DBNICI010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.250"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(67..1254)
                     /gene="tuf"
                     /locus_tag="AC1ZCC_002779"
     CDS             complement(67..1254)
                     /gene="tuf"
                     /locus_tag="AC1ZCC_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HIQ1630982.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            1622..1978
                     /locus_tag="AC1ZCC_002780"
     CDS             1622..1978
                     /locus_tag="AC1ZCC_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HIQ1630983.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT          589 a          415 c          369 g          630 t
ORIGIN      
        1 aatcctgttt ggaccgctct tttattttaa acatttttca ttaccaaaat attcgagtcg
       61 cgtttcttat gcttcgatct ctgtaacgat acctgaacct actgtccgtc cgccttcacg
      121 aattgagaat gtcgttccgt tttcaacagc gattggatgg atcaagtcaa catcaatcgt
      181 cacgttgtca ccaggcatga ccatttctgt atcttctggc aatgtgattg ttcctgtaac
      241 atcagttgtg cggaagtaga actgtggacg atagttgttg aagaatggag tatgacgtcc
      301 accttcttct tttgtcaaca cgtacacttc tgctttgaat tttgtatgtg gagtaatgga
      361 acctggttta gcaagtactt gtccacgttc gatatcgtca cgttggatcc cccgtaacaa
      421 cacgcctacg ttatccccag cttccccgta atctaacgtt ttacggaaca tttctacacc
      481 tgttacaact gctttttgtg tttcaggttt gatccctacg atctctacct catcaccgac
      541 acgaacagca ccacgatcaa tacgacctga ggcaacagtt cctcgacctg taattgagaa
      601 gacgtcttcc actggcaata gtaatggttt atctgtatca cgttctggtg ttgggatata
      661 ttcatctact gtatccatca attccataat agcagcttca gcatctggat cgccttgcaa
      721 tgcttttaat gcggaacctt tgatcacagg agtgtcatcg cctgggaaac catattcgct
      781 caataactcg cgaacttcca tttctaccaa atcgatcaat tcttcatcat cgactaaatc
      841 aactttgttc aagaaaacaa tcaggtattt tacaccaact tggcgtgaca ataaaatgtg
      901 ttcacgtgtt tgaggcatcg gaccgtcagt tgcagaaaca actaagattg caccatccat
      961 ttgtgctgcc cctgtaatca tgtttttcac atagtccgcg tgtcctggtg catcaatatg
     1021 cgcgtagtgg cgtttttctg tttcatattc tacgtgtgcg gtattgatcg taatcccacg
     1081 ttcacgttct tctggagctg catcaatact agcatagtct tgaggatttg ctaggccttt
     1141 tttgcctaat actgtagtga tcgcagcagt taaagtagtt ttcccatggt caacgtgtcc
     1201 aatcgtacca atgttaacgt gtggtttact tctgtcataa tgttcttttg ccataattta
     1261 caaacctcct aaataatata gatcactgaa aaatttcagc tctcaccttt ttattatagt
     1321 ctttttttga ctaaatcaaa aagaatacgt ccgacttact ttttgtaaga ttcgaactaa
     1381 gcttattata cgtcaatatt ggtcaaagtc aactaagagc actcgtctgt cttatacgcg
     1441 aaaaaagtac cttttataaa taaaaaaatg tgttttttga gaaaaacttc aagatagtcc
     1501 ttaactttct tttgaagcaa taagaacaaa atgaggaaaa gtttaaaatt cagctgttgc
     1561 caacaacttt caatagacga aatcttctat ttcgttaata ataaggaaag aggtgaagta
     1621 catgaaaaaa ttaattggcg tacttgtcgt tttaggcata tttctcggcg gaagttttgc
     1681 agcttatcat tatttttacg gcggcgaagc ttattataca aaaatcacga cgaatggcga
     1741 aatgtcagct acccaagcag ataacggaga gaaattcacg acttatacct acaagcaagc
     1801 ggcttataat aaagatggtg aaaaaaaaca ggtgacgctt cgcgaagaaa gagaacatcc
     1861 attaaagatg aatgcgtatc ttaaactcaa agtcaatcct cgtaaaggtg tattaagctg
     1921 ggaagaaata aaggcttctg aagtgcctaa aaatgcggca gaaaaaatcg ataattagta
     1981 aatggtctta aaattttatt aga
//
LOCUS       DBNICI010000241         1999 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.251, whole genome shotgun sequence.
ACCESSION   DBNICI010000241 DBNICI010000000
VERSION     DBNICI010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.251"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..339
                     /locus_tag="AC1ZCC_002781"
     CDS             <1..339
                     /locus_tag="AC1ZCC_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HIQ1630984.1"
                     /translation="IKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKAD
                     WYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTE
                     GLRLHSMGLA"
     gene            499..993
                     /locus_tag="AC1ZCC_002782"
                     /pseudo
     CDS             499..993
                     /locus_tag="AC1ZCC_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
     gene            1026..1409
                     /locus_tag="AC1ZCC_002783"
     CDS             1026..1409
                     /locus_tag="AC1ZCC_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HIQ1630985.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            1485..1616
                     /locus_tag="AC1ZCC_002784"
     CDS             1485..1616
                     /locus_tag="AC1ZCC_002784"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIQ1630986.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            1585..1821
                     /locus_tag="AC1ZCC_002785"
     CDS             1585..1821
                     /locus_tag="AC1ZCC_002785"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIQ1630987.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            1833..1919
                     /locus_tag="AC1ZCC_002786"
                     /pseudo
     CDS             1833..1919
                     /locus_tag="AC1ZCC_002786"
                     /inference="COORDINATES: protein motif:HMM:NF012387.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(1899..>1999)
                     /locus_tag="AC1ZCC_002787"
     CDS             complement(1899..>1999)
                     /locus_tag="AC1ZCC_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIQ1630988.1"
                     /translation="RCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT          569 a          399 c          472 g          559 t
ORIGIN      
        1 atcaaaaatc acgatattat ctgtattgaa gacttaaaca caaaaggtat gttgcgaaat
       61 cataaattag ctaaaagtat ttctgatgtt tcttggtcaa agtttgtgac taaattacaa
      121 tacaaggctg actggtatgg acgaaaaatc atcaaagtag ataaatggtt tccgtctagt
      181 cagatttgtt cagagtgcgg acataaagat ggcaagaaat ctcttgaaat tcgagaatgg
      241 acttgtcctg tttgtcatac tcatcatgat cgtgatatca atgctagtat caatattctg
      301 accgaaggtc taagactaca ttctatggga ttggcttaga tccaatcatc aagaaccgta
      361 ggaactacgg ggatagcttg gtaaataaga gacaccgctg ttagtaaaga aatacgctat
      421 caagtatgct ctattcccaa gaagctccca cttcaagcgt taagaaccgt caggtttagc
      481 taagtggtga gtagttcaca gctggtaagt attcatcaaa atggccgctt tgattatatg
      541 gacgatgtgg ttttaaccaa agatcccatt caaactacca aggaatattt tgatcagatg
      601 ttgcaggtct tgacggattt tccagaagga gatattttga ctcactttga ttatggatta
      661 cgccgcttca ccttcacagc ggaagaattc gcggagcatt ttgaggctca gctgctaacg
      721 attttcaaaa aggttgttga acgagaattg gcgatggagt taaatgccaa gagcttcttg
      781 aaatacggca acgaagacct ttaccgttat gccgttcctt tgtatcaaag tgtcggcgga
      841 aagctcttta ccttaggctc ggatgctcat gttgccgaag attatcagct gggctttaaa
      901 aaaatgagtc agctgttggc agactgtcag gtagaaaagt tgctagtcat tcaaggaaaa
      961 gaacgccgcc tgacgccgct gccgattttg tgattcctat ataatagaag aaagtggtga
     1021 aaaaaatgag caacctgtgg gactacaatc aagaggcacc catccattat ttaattgcgc
     1081 gacattggga tgcgttgaaa attgaggcgg tttgccgatc cttgttagca gccgttccaa
     1141 aacaacaact ggaaaatttt ttggtggctg acagcttgca gcgggaaaaa gttcaggctt
     1201 attttgcagc ctttaaagat cagcctttgg aatatttgca tgcgcagttt catctttttt
     1261 atcaagtagc cgccccagat gactacaacg atttacgagg acagctgcaa ttgacctttc
     1321 aagcggatga aaccgcctat accgttttat tgggaatggc tcgccttggt gatcaggcga
     1381 aggtcgagtg gcggattttc gatatttgat ggcaggatca aggcgtttca gttgcaaacc
     1441 ttcctctctt tcaattagaa aaaccgcagc aggaagaagt ttgtgtgctg gtcaccgatg
     1501 ctggtgaaat tcgtctccgc ttatttcctg accaagctcc cctggctgta gccaactggc
     1561 gctccctagc tgagcaaggc gtttatgaca acacaccttt cggctcgtgt cattaaggaa
     1621 tttgttattc aaggtggcgc tttggatggt tctggtgatg aagctgtttc cagctatgag
     1681 ggctttttcg ctgatgaagt gcatcgcgga ctttatcatt ttaatggcgc cttggcctta
     1741 ggggaccacg gcccccacac caacggcaac cagtttttta ttgtgcaaaa cacaaaggcg
     1801 caggctgacc tgctaatgta aagcaagcat acttggagct tggaggacta ccggagctgg
     1861 atggtcgtta tactgttttt ggacaggtat accaagggtt agaaatcgta gcagcgatcg
     1921 cagctcagcc gacaaacagt gaagatgtac cgattgagcc agtgttcatt cgacaaatta
     1981 acttcgaaaa aacatcgta
//
LOCUS       DBNICI010000242         1966 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.252, whole genome shotgun sequence.
ACCESSION   DBNICI010000242 DBNICI010000000
VERSION     DBNICI010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.252"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(310..1713)
                     /locus_tag="AC1ZCC_002788"
     CDS             complement(310..1713)
                     /locus_tag="AC1ZCC_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HIQ1630989.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTISS
                     TESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPI
                     LSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGP
                     QMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGD"
BASE COUNT          570 a          352 c          308 g          736 t
ORIGIN      
        1 aattaacttt taataaatca gaaaaaaata tttccaattt ttcttagtct gattttatga
       61 ctaatttaca atacaagact gaacgatacg tacacgaaat cctcaagata gataaattgt
      121 tttcatctag tgaaacctgc tcaaaatatg agcataaaga cggcaataat acactcgata
      181 ttcgataatg gacttgtttt atttgtcaaa cccatcgtaa tcgagatatc aattccagtc
      241 tcaatatcct gatcaaaggt ctaagaatcc aaccattttc ttgttacttt gtcagattga
      301 ttaccttttt tagtctccga ataataaggt tttcggatca actaacatgc tctgagaaag
      361 actactatct ttgcaaattt ccaaatgaag gtgtgggcca gtactattcc cagtacttcc
      421 tacatagcca attatttgtc cttgcaaaac attatctcca gtttttacta ggtattgttg
      481 ctggtgagca tataacgcgg ttgatccatc ttcgtgttgt atcaccacgt aatttcccca
      541 agagtagtga aactctgctt taacaacctt accagcttta actgcatgaa tcggctcacc
      601 ctgtgtagct gccatatcaa gtcctcggtg aaattcattt ccacgatttc caaacaaact
      661 ggttattgaa taattatgaa taggcacttg gtatccagtc ttactcattt gtggaccttt
      721 aacttcttta tcatagttgg ttaaatcgta ggttttaatt aattcattta gcttcaaata
      781 gtattgtgta tctgttgcat accgtcctgt aaggaattta gttgcttctc gatatgtttt
      841 actattagct tttaatgctc ctgcataaaa atctttattt ccactaattc ccttagttaa
      901 taatttggca taatcttcaa agctttcctc atagttttca tacactctaa attttgattg
      961 ggtcgtataa agcgttccat tacccaaatc ttcgtttgtt accataatga caaagtttcc
     1021 attataagtt ccttttatcc caaataaatt gtaattcggt gcctgagcta actggctttg
     1081 accactggca gattctaaga tcgcttgtgc aatcataaca gaagcatata aatcatattt
     1141 ctgaccaatt tttctcgcag actcaccaat tttcataata aaattttcta cagattcatt
     1201 tttgtcaaaa tgaatttctt cattaccaga tagtattggt tgtgattgga aagattctgt
     1261 tgattctatt tgctgtggtg tcgaaaaagt ttttgtttgt ccttccgttt ttggcgcttc
     1321 cttttttgaa gattctatat ggtttggttt tgtcactttc gaagaagtta tttcacttgt
     1381 actacttgaa gttgttgaag attcggtact tgaaatcgta ctactttcac ttgacgaagt
     1441 tgttgaagac tcggcatttg aagtcgtact actttcactt gatgaagttg ttgaagactc
     1501 ggcatttgaa gtcgtactac tttcacttga tgaagttgtc aaaagttcac tactctctgc
     1561 tgttacatcg gaattactca tttcagtcgt ctcggttctt tcttttgcaa ttttgtatgt
     1621 tgaattttcg cttgccaaca catggaaagg tgttgctata aggagttggg acattaatat
     1681 aactgctgat gcaagtttta aagatttttt catttgattt acctctcttt tttctcttta
     1741 atagtgtttt ctattaaaaa ttatgataaa tattttcaat gaattgagta atacagtata
     1801 tttcttgatt tcatttagta acgattatgt ttcaatgttt aatcctgtta ctgcctttaa
     1861 aacgccgatt gtaaaattaa gctagacaac taaaaaaagt catttgtgct acactcaaaa
     1921 tagttccgac gaaagaatta taaggagtga gtacaaatga cctata
//
LOCUS       DBNICI010000243         1902 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.253, whole genome shotgun sequence.
ACCESSION   DBNICI010000243 DBNICI010000000
VERSION     DBNICI010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.253"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..770)
                     /locus_tag="AC1ZCC_002789"
     CDS             complement(<1..770)
                     /locus_tag="AC1ZCC_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1630990.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMIFGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(763..1698)
                     /locus_tag="AC1ZCC_002790"
     CDS             complement(763..1698)
                     /locus_tag="AC1ZCC_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1630991.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
BASE COUNT          591 a          431 c          345 g          535 t
ORIGIN      
        1 taacttctca atcctcttcc tttattgatc aaataccaag taacggcgta caaaacaacg
       61 atgaatagca caagtacgat catcgatgtt gctactggca catcgattgt tccaaggaat
      121 ccgtaacgga aaccgaagat catataaacg atcggattga ctttcgaaac agcttgccaa
      181 aatggcggca acatagagat ggcatagaaa acgccaccta gataagttaa cggctgcaag
      241 acaaatgtag gcacgatcga tacatcgtca taagactgag caaaaatccc gttgagcagt
      301 cctgctaacg aaaacaagat tgccgtcatc aataatgtaa tgatgaccat gaaccatgaa
      361 tacacatgaa gtggtacaaa gaacaaggaa atgatcgtta cgagagagcc gacgagaata
      421 ctgcgaccca ctccgccaat cacaaagccc caaatgatca catgcgtagg aacaggcgct
      481 accaagattt cttcgatgtt tttctggaat ttttgagaga agaaggacga agaaacgttg
      541 gcatacgaac tagtgatagc agacatcata atcaaaccag gcacgatgaa ttccatatat
      601 gaaaaaccac ccatgtcacc gatccgacca ccgatcattt ttccgaaaat aacaaagtac
      661 aaggatgtcg tgatcacagg cggaaccagt gtttggaccc agatacgcaa atagcggttc
      721 gtttccttga tagctaaact ttttaatgca gtaaaataaa gattaaacat ttttctcctc
      781 cgtatgatta ttttcttctg tgattttcaa aaacagttct tctaaacgat tcgacttatt
      841 cctcatagaa agaactttga ttccttgtct tgtcagctga tcgaagattc cgttcacccc
      901 ttgatttcgt tcaacttcga cagcgattgt ctgttcatct tcaaatgtac aagcgtatcc
      961 ttcaatcaca ggtttggtct catatggcgc taaatcaaag ataaatgttt caaattgcag
     1021 tttggataga agctctttca tactagtatt ctcgatcagt tcacctgatt ggatgatccc
     1081 gatgttgcga caaagcattt cagcttcttc taaataatgc gtcgtcaaaa tgatcgttgt
     1141 accattttcg ttcaattctc ggagaaactc ccacatctcg cgtctcaatt caatatcgac
     1201 tcctgctgtt ggttcatcca agatcagcaa acgtggttca tgcatcaatg ccctagcaat
     1261 catcagtctt cgcttcattc ccccggaaag catacgtgca cggacatttc tcttttccca
     1321 aagattggac tgcttcaaat atttttcgct gcgtttcatc gcttctttgc gagagacacc
     1381 gtagtagcct gcttgattca cgacgatttg ctggaccgtt tcaaagggat tgaaattgaa
     1441 ctcttgcggg actaatccaa tctgttgctt tgctcggact agatccgtat ccagatcata
     1501 accgaagaca ttcacttttc cagatgtttt attgactaaa gaagtaatga ttccgatcgt
     1561 tgtagacttc ccagctccat ttggtcctaa tagcgcatag aaatctcctt ctttcacgga
     1621 aagatcgatt cctcgcaatg cttcgacccc acttccataa acctttttca aattatttat
     1681 ttcaagcgca tttgccattt atttcctatc tccttataga catgattctt tattgtatca
     1741 tggaatacag cagatgaaaa aacaacttgg ttccagcgaa aaatcaggtt gctgggctca
     1801 ggaaaatctc ctaatgaaag acatcaatcg gacgaagtca gacaaccttt tcagaaaaaa
     1861 caaatcgaag ataaaggatt actattcata tgtgcaacaa aa
//
LOCUS       DBNICI010000244         1812 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.254, whole genome shotgun sequence.
ACCESSION   DBNICI010000244 DBNICI010000000
VERSION     DBNICI010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.254"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            77..1237
                     /locus_tag="AC1ZCC_002791"
     CDS             77..1237
                     /locus_tag="AC1ZCC_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HIQ1630992.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            1253..1696
                     /locus_tag="AC1ZCC_002792"
     CDS             1253..1696
                     /locus_tag="AC1ZCC_002792"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1630993.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
BASE COUNT          633 a          287 c          365 g          527 t
ORIGIN      
        1 ggctttcatt ttctattttt ttcctgaacc catataataa aagaaagaac aaaaaaactt
       61 gcaagggagg acacatgtga agcagagtaa gcaaaaacag tctcgtcgac ttttgtttcg
      121 tttgttttta ttaatatgta tcgtcgtagg gttactgatc gctttatatc cattttatgt
      181 ggattcttta aatagtttta ttgatcaaaa acgtatcgaa caagtacaaa aacggaccgc
      241 agctgaaatt gaagtgcagc gaaagaaaat ggaggagaaa aaccaacgat taactgacca
      301 aggattcaat cctggtactg atcctttcaa tgaaaagaac agaaatgaaa gtacaacatc
      361 cagccaatta gaggaatggc tgatcggaag tatcaatata cctaagatcc aattaaatat
      421 ttcactttat gatcggctca atggtaggat ccttgaaaat ggagcaggcg tcttgcaagg
      481 aacctctttc ccgcttggtg gaaattcgac gcattcagtg atctctgctc acagcggttt
      541 acctaatcga cggttattta ccgaattaga tcgtttagaa aacggagaca cgtttatttt
      601 gactgttttg ggggagaaat tggcttatca agttaaaaat atacaagtgg tactgccaga
      661 tgacacgtct gttctaacga tttcagaagg agaagactta gttaccttgc ttacatgtac
      721 gccgtatatg atcaacacgc atcgtttgct agtgacagga catcggattc cttataacga
      781 atcggtaaaa aaagaagaag gaaaaggaaa tcaggaaaga accatgcggc aatggtttat
      841 tcttgcagga acgataatag ctgtagtcat actgttagtg tttatcggac gactaatcta
      901 tcagtataga ttaagtaaaa aagtgcttga tttttcattt attattagtg attcgtcagg
      961 gaaaccggta aatggtagtt cattcatttt gaaacataag aaaaaaacac ttactagaaa
     1021 tggtgtgccg ttttcagtac aaagcgacca tcatgggaaa gtcaagctgg atcaattgcc
     1081 tggtggaact tatcgaatcg tttcagctga tgatcctaaa gttgctgttt cttttggtat
     1141 tagaaaagta aaacaagaga agatatactt ttttgagggg cgaaaacttg taaaagagct
     1201 tcaaaaaaat ggtttttatt ttaaaatcaa tgaataagta aggagagaaa aaatgatccg
     1261 cacaacaaca aaattgtctg ccaaagaact ggaatctgtt ctatcgattt ggctgcatgc
     1321 taacttggat tctcatgaat tcattccatc aacttattgg gagaatcaag tagaatctgt
     1381 taggaaagct ttaccagatg cagtgattta cttagcagaa atagatcaag aaattattgg
     1441 ttttgctggg gtgattgatc agtttatcgc aggaatcttt gtgaaaaggg aataccggca
     1501 acaaagaatc ggtacgcaac taatgaaaaa aataaaagaa accaataaaa cactgacttt
     1561 gacagtctat caaaaaaatc aatcagctat tcatttctat ctttcacaag gttttacaat
     1621 tcaaacacag caaaaagatg aagcagtaaa tgaggaggaa cttgtgatga aatggaatca
     1681 aaacgctgat ttttgatgaa cagaataacg aaataatgct cccagcctaa ttttttgctg
     1741 ggagcattat ttcattttga agtcactaat ttaactgtgt gaatatgcag ttgttacaat
     1801 ctttgataaa cg
//
LOCUS       DBNICI010000245         1803 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.255, whole genome shotgun sequence.
ACCESSION   DBNICI010000245 DBNICI010000000
VERSION     DBNICI010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.255"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            165..800
                     /locus_tag="AC1ZCC_002793"
     CDS             165..800
                     /locus_tag="AC1ZCC_002793"
                     /inference="COORDINATES: protein motif:HMM:NF012655.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIQ1630994.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            907..>1803
                     /gene="mef(H)"
                     /locus_tag="AC1ZCC_002794"
                     /gene_synonym="mefH"
     CDS             907..>1803
                     /gene="mef(H)"
                     /locus_tag="AC1ZCC_002794"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HIQ1630995.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSV"
BASE COUNT          612 a          251 c          300 g          640 t
ORIGIN      
        1 cagtatgaat tgacaccgca taaggaaagc aactggaaga ttgtagaata aatgaaaaag
       61 gcttgcattc tgcattttta taaaattgca agtctttata ttgacaagca taaaatacta
      121 agatatactt taatcagacc aacggtttgt attggaggta agttatggca cgaaataaat
      181 atccagaaga aacagtaaaa ctcattttag atgtttctac aaaaatgttt tgtgaaaaag
      241 gatatgataa cacatcattg caagatatta tcaatgaaac aaaactttct aaaggtgcta
      301 tttatcatca tttcgattca aaggaagata ttttaaaagc aatttttcaa agaattggaa
      361 atgaaaatgc agtaatattt gctaaaatta gagataacga tagtttaaac ggtttagaaa
      421 agttaagaga aatatttaag acagcggtat tcaattctaa ccaaagcgtt ttattgacag
      481 tttctcctca actattaaac aatcctcgtt ttcttgctat gcaaatagaa caaatgtatg
      541 aaattgtagt acctgaattt gttgaacccg ttttagaaca aggtatcaag gataagtctt
      601 tggagattga aaattcacat gaaattgcag aagttatcat ggtattaagt aatgtatggc
      661 tcaacccatt agttgaaatg acggataaag agggtatgga aaaacgttgc aatacattca
      721 atgccctttt aaatggaata ggcatagaca cattattaga cgaagaaaca atttctgcat
      781 ttatacagtt ttgtaaataa ctgtatttgt tatccctata cgatagactg ctctataaaa
      841 gagcagttta tttaaaccat tatataaacc gacggtctgt ttgattaaat aaaaaggaga
      901 gattttatgc aagcagaaaa aaaacttttt aatcgtgatt ttatacttgt tgttatagga
      961 cagataattt ctttatttgg aaacgctatc ctacgatttg cattaccgta ctatttgtta
     1021 aaagaaactg gttcatcagc tttgtttggt attgctacag cctgctcttt tttacctatg
     1081 gttattttgt cgctggtagg tggtattatt gctgacagga caaataaaag aaacattatg
     1141 gtcatactag attttgtaac agcaatcata attttagtat tttatttgtc attgggaaaa
     1201 attcctatca tacctttgtt tattgttgct ttgatgttac tttatggtat ttctggtgca
     1261 tatcaaccag cggtacaagc aagtattcct ctattggtat catcagataa acttaccgtc
     1321 ggtaatgcta taataaatca agtaaatact ttagcaaact ttatcggacc agctataagc
     1381 ggagtagttt ttcaatttta tggtatcaat cccattttaa ttgttagtat tatatgtttt
     1441 actttttcag ccattatgga aatttttatc catattccac accaaaaaag acctcgtgaa
     1501 gtaggtgtaa ttgcagttgc taaaaacgat ttaaaagata gttatacctt tttgaaaaag
     1561 gagaaaccca tttttatatc cgtaatcttt ttggtttgca ttttcaattt ggttttaagt
     1621 gctgttttaa ttgttggtct gcctatctta atttctgaag ttttaaaaat gccagataca
     1681 ctatatggtt tttcacaatc agcattatca ttaggtggtt tatgtggtgg tattttaaca
     1741 gctcttgttg cttcaaagtt aaaactaaaa aacctttata ccttgttact tatttgttct
     1801 gtt
//
LOCUS       DBNICI010000246         1797 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.256, whole genome shotgun sequence.
ACCESSION   DBNICI010000246 DBNICI010000000
VERSION     DBNICI010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.256"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            98..1732
                     /locus_tag="AC1ZCC_002795"
     CDS             98..1732
                     /locus_tag="AC1ZCC_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HIQ1630996.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNM
                     VKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPN
                     KDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTI
                     RYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT          633 a          230 c          236 g          698 t
ORIGIN      
        1 aaaatatttt attaataata aaattaatca cattttatat agtgactaat ctagtgttta
       61 aaaaaaataa atgatactat atgtaatata aaaaaacatg aggtggtata tgaatttgaa
      121 aaaaattttt tctgtaaaaa tatttatagt ttatgcattt gttagtttat tatatttatt
      181 tccagcattt aaaaacatgg aaatatttgg cttttatgat acggcatttc acattaatcg
      241 tgcgttaagt ttagaatcta tttttagctc ccctattaat tttgaaacgt ttagatcata
      301 tggcatgcaa gtaaataatt tttacccatg gttaactcta tatcctttat ttttattgat
      361 caagtttact aatttagcaa taggctataa cctttttctt tatattgtta cgctcattac
      421 tctatttata tgtcattatg taatgtatga aattaccaaa aaacatgtta ctagctcctt
      481 tttcgctatt atatatacaa catcttcttt taggagtgta gaaatatttc ttcgaggggc
      541 aatgggggaa ttactcgcaa tgtcaatact tcctcttatt ttactcgggt ttataaagct
      601 ttacgactca aaaaaagaat cttgggttat gttggcgatt agcatgactt tattaattta
      661 cactcatgtt ctttctgtag ctatgactat ggtaatgatt ataattgctt taattattca
      721 attttatcgg aaaaagaaaa tagaaatctt cttaattatt aaattaataa aagctgctat
      781 agttacttta cttctttcac tagcgtttat aggtcctatg attgagcaga cattgtatca
      841 agatctgaat agaccttatg tggatatatt acaaaaaaga gcaatttatc tgggtaaaga
      901 gtttttgatt ggaagtatag attcagaatt actatcattt ggtattggct taactttgct
      961 tatttgttta attatactaa catttaactt taaaaaatta ggaatcttat ctaaaatcac
     1021 ttatataatg ggtataatat ctattctact ttgtacaaaa atatttccat ggtttgcctt
     1081 acaaaatact cctttaaata ttattcaagc tccttggcgt tttatgatat tttcaacatt
     1141 gttcatatct ttttcagtat ctctatttat ttctccacag ttggaaaaat tttcttatac
     1201 aaggagaaga aattttatac tattattaat attagctatt tcaattttaa attattcatc
     1261 agtagttaac atggtgaaag aaatgaattt tcaaggaact tattctaatg aacaaatgga
     1321 aaataaactt gaagaatttg agaattttga ttatgtacct ataggctata aaaaagatca
     1381 attgcttttg aaaaaccatc aagttacaga agatggtcaa gttatttata gtagcttttc
     1441 tccaaataaa gatgaaatta catttacgtt taaatcttca aaaggcaaaa caatagtttt
     1501 acctgtattc cggtataaag gattaacggt tactgttaat tcaaaaaatg ctacagtact
     1561 aaaacaagga ggtccttttg ttacagtaaa aagtgaaagt ggaataaata aagtaacaat
     1621 caggtattca tacacatttt tttctaaaat ctcattattt atttctataa tcacgctaat
     1681 tgttcttctt ttttattgca taaaattaaa aataagaaca agccatgaat gatttgctag
     1741 cacatatttt agcaatctaa agatactatt ttagatttaa taggcccttt gtcaaat
//
LOCUS       DBNICI010000247         1773 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.257, whole genome shotgun sequence.
ACCESSION   DBNICI010000247 DBNICI010000000
VERSION     DBNICI010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.257"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..803)
                     /locus_tag="AC1ZCC_002796"
     CDS             complement(<1..803)
                     /locus_tag="AC1ZCC_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIQ1630997.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSFVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKF"
     gene            complement(820..1653)
                     /locus_tag="AC1ZCC_002797"
     CDS             complement(820..1653)
                     /locus_tag="AC1ZCC_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HIQ1630998.1"
                     /translation="MSDNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIDVLDNGAIYMSDI
                     QSFIGKSSTEADRKREYRKKIEEAKRNLITGGQVSDKCPDKTTPELEIEIEKDIDIDK
                     EEKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEY
                     VINWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLP
                     I"
     gene            complement(1660..>1773)
                     /locus_tag="AC1ZCC_002798"
     CDS             complement(1660..>1773)
                     /locus_tag="AC1ZCC_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HIQ1630999.1"
                     /translation="IHHTEKHNIGVINFKNKYQIKGIKLNQETIKKLRIGG"
BASE COUNT          446 a          361 c          256 g          710 t
ORIGIN      
        1 tggaatttca gcgcataacc ttctgaattc ttaaatattc tagaaacaat tctttcgccg
       61 taggcttctc ttagttcagg accggataag tttgttgtga tgatcgttgc cttgttctgt
      121 ctggcttcta agagcgtgtt taacgtgttg tttgtaaact gcctactatt tgataccccg
      181 ctacctaatt cagctccaat atcgtcaaaa accaccaaat cagttgtttt gatatcggct
      241 ataagcgatc cttcaatttc ttttctcagt tcagcattgt tataagaaaa ctttatttgc
      301 tctaataact cttgatagct tataaaaagt attttcttgt cataatttga gcgctcaagt
      361 atttcccaag ctgtcgccat tgacaagtgg ctttttccgc ttcctgattt ccctgataga
      421 atgaaatgtg caggatggtt cagtaagaca tcatttacaa agcttttagc tctttctaaa
      481 gcaattttcg tttcttggtc cactacgtga taattctcca ttttgcattt aaacaaagtt
      541 ttatctgtta ataccgaacc attttgaaaa aaactcaacg ctcgtgcttt taagctgtcg
      601 ttatatatcc gttcggtctg tatatcctct ttcacacgta acgctttata accacaactc
      661 atgcatgttg gtttacaacg ttctgaacca tccttatttt tagctcgcca actatacaaa
      721 ggttcgctac attctggaca tttcccgctt tgcactaata ctcttcttat tagtttctcc
      781 atagcatttg ctaggctttc catgtgatgc atctcctttt taaattggca agtcgtcata
      841 ttcactagga ttgctgtact gtagtttttg actttgcttt ttatgattat tcttgtctgc
      901 tttgatttcg aatttgagct tctcaaattt ttctctcaat ttcttagcac ttctaatatt
      961 tccaaaccaa aattcatttg taggtagcca attgatcaca tactcaatcg cttctataga
     1021 cgctttatct ctttcttcca tcaacctgat tgtgtctgcc catttttcga tatctacttt
     1081 gttcatttct tttggaaaat cttcagttaa attactttgc aattttttag caaggcgtaa
     1141 gtgttcgtca gaatacttac ctttcttttc ttctttatct atatctatat ctttctctat
     1201 ctctatctct aactctggtg tagttttgtc tggacatttg tccgacactt gtcctccagt
     1261 tattaaattc cgttttgcct cttctatttt ctttctgtat tctctttttc tatctgcttc
     1321 agttgaggat tttccaatga aactttgtat atcagacata taaattgctc cgttatctaa
     1381 tacgtcaata agctgcaaat cacggaaaat ttgtaccgct ttttctacga ctcctacaga
     1441 atgtcttgta atagttgcga gcattgtaga gttaaatgga atcctgtcat taaacatcaa
     1501 cttaccttcg tgttttagac ttcttaaata aagtttgaga agaatattag aataaatata
     1561 gccatctggc atactttcta agagaaccat ctcgtcacta tcgaaaaaat tctcttttag
     1621 ttttaaatag tagtagcgtt tgttgtcaga caatattttt taccctccta ttctaagttt
     1681 cttaattgtt tcctggttta acttgatccc tttgatttga tatttatttt tgaaattgat
     1741 cacacctata ttgtgtttct ccgtgtgatg aat
//
LOCUS       DBNICI010000248         1736 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.258, whole genome shotgun sequence.
ACCESSION   DBNICI010000248 DBNICI010000000
VERSION     DBNICI010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.258"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(192..1484)
                     /locus_tag="AC1ZCC_002799"
     CDS             complement(192..1484)
                     /locus_tag="AC1ZCC_002799"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIQ1631000.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          498 a          366 c          303 g          569 t
ORIGIN      
        1 ttcggaaaaa gggactgtga cacggttttt gtcacagtcc cttattctga ataaacagtg
       61 ttcaagagac agcctatcgg caataaggct aaacgtctct ttcactacct cttcaagtta
      121 ttattatcgt atagataaac acttcgactc gctctggtaa gaacgtcctg attcagtcgt
      181 cgtttttatc cttagatttg cgcccagacg ctttctaaga tgtttgtttg tgttctatca
      241 ggtccgacag aaaatgttga gatacgtaca ccaacaagct ctgagatacg acgtacatag
      301 ttacgtgcgt tttctggcag ttctgcaagt gttttgcatc ctgtaatgtc ttctgaccag
      361 cctggcaatt cttcatagat tggtttgcag cggttcaact ctttcaagct tgctggatag
      421 tggtagatta gttcgccatc taattcataa gccgtacaaa tttttactgt ttccaatcca
      481 cttaatacat cgatcgagtt taaagatagg ttggtaatac ctgaaactcg tttagaatgg
      541 cgcataacta cagaatcaaa ccagcctaca cgacgcggac gaccagttgt cgtaccgtat
      601 tctcgtccta cttcgcggat ttgttctcct gtttcatcaa ataattcagt tggaaatggt
      661 ccatcaccga cacgtgacgt ataggctttg cagacgccga caactttatt gatttttgaa
      721 ggccctacac cgcttccgat tgttacccca ccggctactg ggttagaaga agtaacaaaa
      781 ggataagttc cctggtcgat atccagcatt acaccttgtg cgccttcaaa caacacgcgt
      841 ttgcctgcat ccaaagcatc attcaagata acggaagtat ctgtgacgta ttgtttgatt
      901 tgttgtccgt attcataata ttcttcaaaa atatcgtcaa aatcaagtgg ttctgaatca
      961 aacattttta caaattgacg gtttttgtca tctagattca aacgaagacg ttcttcaaaa
     1021 atttctttat ctaataagtc tgcaatacgg atacctacac gagctgcttt atccatatag
     1081 gcaggtccga ttcctttaat cgttgtacca atcttatttt cgcccttagc atcttcttgt
     1141 aattggtcca atttgatatg ataaggcaag attacgtgcg cgcggtcaga aatacgcaaa
     1201 ttacttgttt ctacaccttt ttcatgtaaa taagctaatt ctttaaccaa tgatttagga
     1261 ttgactacaa cgccgtttcc gatcacactg atcttgtctt tataaaaaat cccagaagga
     1321 atcaaatgca gtttataagt cacaccgtta aacttaattg tgtgccctgc attgtcgcca
     1381 ccttggtacc gtgcaatcac ttcagcattt tcactgagaa agtctgtgat ttttcctttc
     1441 ccttcatcgc cccattgcgt acctactact actacagatg acatgtgaac acctcgttct
     1501 atattattta atcctgaacc attgtatcaa taatagatac atatttcaaa taaaatcgaa
     1561 tgatttttat ttttatttta taaaaacgct taaaaaacga actttatctt tttagaatgg
     1621 ttattaaaaa agaggttgta acagaagtgg tcagcttcaa gaaataagaa ggaatgtacg
     1681 aaaattgctt ctcaaatttt tgtgaaattt cgacttattt ttcgaagaag ctactt
//
LOCUS       DBNICI010000249         1699 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.259, whole genome shotgun sequence.
ACCESSION   DBNICI010000249 DBNICI010000000
VERSION     DBNICI010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.259"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..779
                     /gene="dcm"
                     /locus_tag="AC1ZCC_002800"
     CDS             <1..779
                     /gene="dcm"
                     /locus_tag="AC1ZCC_002800"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HIQ1631001.1"
                     /translation="EGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERIFLIGHS
                     RDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGDEGIKIA
                     LPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQEVKPGN
                     SVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQGFPDWAF
                     DRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            804..1058
                     /locus_tag="AC1ZCC_002801"
     CDS             804..1058
                     /locus_tag="AC1ZCC_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631002.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            1314..>1699
                     /locus_tag="AC1ZCC_002802"
     CDS             1314..>1699
                     /locus_tag="AC1ZCC_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1631003.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGV"
BASE COUNT          532 a          317 c          420 g          430 t
ORIGIN      
        1 acgaaggagg ggctacgttc gagacgatcc tccgaacgct ggatggattg gggtatgatg
       61 tggaatggca agtgcttaac tcaaaggcct acgttcccca aaaccgtgag cggatcttcc
      121 ttatcggaca ttctcgagac gcatgtaccg aacaagtatt tcctatcatt ggatcgtctc
      181 caacatctga tcaaaacatc agaaacttat taaatatcaa cccttccaat cgaggaatgg
      241 ggggacaagt ctatgggtca gatgacgtag cgcctacctt gaccggtgat gaaggaatca
      301 aaatcgcatt gccggtagat gatggaattt ctgtagcggg tatgttactg ggaaattttg
      361 aacaaggcaa tcgggtttat gagataactg gaacagcgcc tactttgtca acgaaacaag
      421 gcggaacgaa gatcatgctt cggaaaaacg aaacagaata tcaagaggtg aagccaggga
      481 acagtgtgaa cctggctttt cctaattcca caagtcgtcg aggccgtctg ggaaaacaaa
      541 gtgtgcatac cttattaaca ggagaccaac aagcagtcgt cacggatcag tatcaaattc
      601 gaaagttaac acctagggag tgctggcggc ttcagggctt tccggactgg gcttttgatc
      661 gagcttctca agtcaattca gatagtcagt tgtataagca ggctggaaat tctgtaacag
      721 ttccggtcat ttttgatatt gctagaagat taaaggaggt aaaaacagat gatttatgat
      781 gagttgattg gggaatctac tgggtgattg gcaagattca gtcagatccg gaattggaag
      841 aagaactgta ccggctcaat tttgatattc gcaaaaacgg ggtgaaagtc cctgatgatc
      901 cgtatgtaat tgatgaggaa acggatgctc gaatggagct gaatcaagtc atcagtgaat
      961 tggaacgaat tgcggacttt gctaaagaac ctgatatccg ccaatacttg tttgagataa
     1021 aagcagagtt ggaaattgag ggtattaccg cggagtaacg gccaaataaa ccactgatag
     1081 gcactgaccg cgggggattt gcgaagcaga aatcctccgt ggtgctgtgc ctgtttatgg
     1141 acggcgttag ccggccatga tgtctcaccc cccgtatcta acaggggggt acaaaaacac
     1201 aaaaaataag caagcaacga taaggaatgg ctgtcctatc aaggataata agcaatttgt
     1261 gtagatgtta actgatttga aaaagttaac accttaaatt tggaggtgag aacttggcac
     1321 aaaggaattt agattatcgg ctattaaaag accggcgtaa tgaatatggc gtttcacaaa
     1381 ataaattagc tacgacttgt ggacttagtc gcccgtactt aaatcagata gaaaatggtg
     1441 gcgtaaccgc atcgacaaaa accatgagga aaatttttga ccaactggaa agtttcaatc
     1501 ccgagttacc tttaacgtta ctgtttgact atgtaaggat tcgctttccc acaacggatg
     1561 cgaggaaaat cattcaagag attctccatt tgaaatttga ttatatgctc cacgaagatt
     1621 acgcctttta ctcctatcaa gaacaatatg ttatgggaga tattgtggtg atgttgtcac
     1681 atgaagaaga taaaggcgt
//
LOCUS       DBNICI010000250         1698 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.260, whole genome shotgun sequence.
ACCESSION   DBNICI010000250 DBNICI010000000
VERSION     DBNICI010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.260"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            31..1056
                     /locus_tag="AC1ZCC_002803"
     CDS             31..1056
                     /locus_tag="AC1ZCC_002803"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0016019 - peptidoglycan immune receptor
                     activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045087 - innate immune response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein family
                     protein"
                     /protein_id="HIQ1631004.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            1246..1319
                     /locus_tag="AC1ZCC_002804"
     tRNA            1246..1319
                     /locus_tag="AC1ZCC_002804"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1280..1282,aa:Ile,seq:cat)
BASE COUNT          557 a          286 c          340 g          515 t
ORIGIN      
        1 ctcggctgaa agcgaagaag gaggaaacaa atgaaaaaga aaattactat tactgcgatg
       61 agcctgttaa cggctctttt tttattaccc attaatacgt ttgcttatac tattaatgac
      121 gagtataatt tagcgccgaa tcaaggagac tccagattag caattcctaa caaaattatt
      181 ttgcacgaaa ctggaataga tgcaccagca agaaacgtag ccgccaacat gaaaaataat
      241 tataacggaa gcaattctta tactacagat gttattggtg acggtgggat tgtttaccgt
      301 gtgggtgagc aaggatatgt ttcgtgggga gctggtaacg ctaatcctta tgcgcctgta
      361 cagattgaat tacagcgcac atatgataaa gcattgtttg aaaaaaacta tcgagcttat
      421 attgaatata caagagatag tgcaaaaaaa tatggaattc cattgactct tgatcaagga
      481 acttctttat ttacaaaagg aatcatttct catttgtggg tgacaaatta tgtttggggg
      541 aaccacacag atccatatgg ttacttatcg caaatgggag ttagcaaaga aaagcttgct
      601 tatgatttag ctcatggatt taccgatgaa aatccaacaa cttctgaaaa caagcctgtc
      661 attgatccaa cacgagctgg tgcagctaat cctacgctga cagatggaac aaatcacgcc
      721 cacattgatc agtttgggga aatcgaaaac gcaaacttgc atgtggctgg atggcacatt
      781 gctaactata aatacgagta tattttcatt atggactaca atactgggaa agaattagct
      841 cgagtaagag ctgatggaat ttatagacca gatgtaaacc aagcttataa tacttctgga
      901 aatgttggtt atcatgtatc tttcaatatg cgtaattttc ctagtaagaa agtctatgta
      961 atgatgcggg caacgaatga tccagaggga aacactaaag gcggtgcgca agatttccat
     1021 gacaaacgtt ggtatttaaa tattcctaaa cgataaaaat agctcctcgt tgaggagcag
     1081 tacataacta tattgacaac tataaaaatt attcgataaa atagtgatgt tatcgcatat
     1141 cttcactatc acccataaat agtcacactc caagctatgc gataacaggt ttgttgccac
     1201 acattctact ggttgattgt ttatggcttt atgtggcaac aacctgtact cttagctcag
     1261 ttggtcagag cagacggctc ataaccgtcc ggtcgtaggt tcgagtccta cagggtacat
     1321 aatttaatct atttgataaa aaataaaatt caataatggt ataataaaat gtcggctcgt
     1381 cgcttccaca gagattttcg tgggaggagg tgtatgattt gtttgaattc actttggagc
     1441 acgtgctctt gccattgtta attggtttaa ctttggcatt atttagtcat tggctagata
     1501 aacattagag ccgacaatgg cgcttactgt ttcgcagcgg taagcgtctt tttttgaaaa
     1561 agaaaaaaac acaacgtatt cgcaggtacg ttgtgtttat ggtgtatatt tgtttaaatt
     1621 cactttcaat tattattata cttgttaaat aaattgtgtc aatataaaag atatttttat
     1681 acgtgtacta acctatat
//
LOCUS       DBNICI010000251         1667 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.261, whole genome shotgun sequence.
ACCESSION   DBNICI010000251 DBNICI010000000
VERSION     DBNICI010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.261"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            11..205
                     /locus_tag="AC1ZCC_002805"
                     /pseudo
     CDS             11..205
                     /locus_tag="AC1ZCC_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(393..1169)
                     /locus_tag="AC1ZCC_002806"
     CDS             complement(393..1169)
                     /locus_tag="AC1ZCC_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIQ1631005.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            1292..>1667
                     /locus_tag="AC1ZCC_002807"
     CDS             1292..>1667
                     /locus_tag="AC1ZCC_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIQ1631006.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEKFLIRNYKISGGGC
                     YLLECRFTSKKSMNSFLEQLNEY"
BASE COUNT          563 a          277 c          253 g          574 t
ORIGIN      
        1 aactacttaa gatgaagcag aatatttacg ccatacggca tacaaccgcg aaaaatataa
       61 aaaaaagaaa acgattgaac gcgtttttgc agtcgcaaag gaaaagcatg gaatgcgcta
      121 catcaagtat cgagacttgg aaaacaatca ggtgtatacg ctgattgttt ttgctgcaat
      181 gaatctgaaa aagttgtcca cttggcagtt ggaacatatg tcttcatatc ttttattata
      241 caaaaatagc aaaagcacca gtctacactc ttagtaaaga ggaaactgat ccttttgtct
      301 acattcttaa agtacttaaa ctaagtactt tattttactc tttaggattg atacagtagc
      361 atctatccct ttattaataa tcttttattt ttttaaataa agatgttgtc tccatcttca
      421 ggcactaaaa ctttttcgga aattcctttt tctttattga aagactttaa ttcttctctt
      481 gacagtcccc aatggtttac agcttccata tgaacggcaa taattgtgga atctggtatt
      541 gctttatgca attcatataa atcgtctttc cccataataa gagattctcc aactaaaaaa
      601 tgattatctc cagcatttgc tactatgatt tctggattat actgatcaat agtttgttta
      661 acagcttcat accaaactgt atctcctgta aagtatagtg tcttctcagt cgaatgtttg
      721 aaaacaatac cgcaaacatt acctacataa ttcaagattt ctcctctgcc atgtttgcct
      781 tttgttttaa ccaattgtat agattcaaaa actgtgtctt ctcgtagtac ctccacattt
      841 ttaaaaccat atagtcgaat ttcattcgca tcattttcat cttgcacaaa taattttatt
      901 tctttaggta ggacctcttt cgctatatcg tcccaatgat ctagatgtaa atgtgttaca
      961 ataacagcat caataccgtt gataatatct tcgactggca ttggcaaatc aactagagga
     1021 ttcatttgat cttttcttgg tgatggaaaa ggacctttcg aaataaatgg tggaagtgtt
     1081 cctttttccg ctaacattgg atcaattaaa aattttttac ctccatagtc taagatagct
     1141 gttgcattac gaatgtgatg tatattcata aacgttgtct cctttttgtt gatatgaaat
     1201 atttttagta taattcaatt ccatacaata aataaaccga acaaaaaaag tcttttacta
     1261 tttttactta atttatgaaa ggttgtttac catgttagac caaacagata taaatatcat
     1321 tgaggaacta tcaaaaaata gtcgaatcac aatgaaagaa cttggagaaa aagtacattt
     1381 gactggaccc gctgtctcgg cgagagtgac aaaactagag gaaagtggag ttattgaaaa
     1441 ttatacagta aaattaaata tggaaaagct agggttttcg attcacgctt ttttaattat
     1501 aattacgcaa aatttaaatc ataaacccta tttatcattt attaaaaatc atgaaaaatt
     1561 cttgattaga aattataaga ttagtggagg gggctgttat cttttggaat gtagatttac
     1621 ttcaaaaaaa tcaatgaaca gctttttgga acagcttaat gaatatg
//
LOCUS       DBNICI010000252         1653 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.262, whole genome shotgun sequence.
ACCESSION   DBNICI010000252 DBNICI010000000
VERSION     DBNICI010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.262"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..1238
                     /locus_tag="AC1ZCC_002808"
     CDS             <1..1238
                     /locus_tag="AC1ZCC_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HIQ1631007.1"
                     /translation="RQFDISKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQ
                     DHWRSLWHRQKLARMILENHWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDG
                     LLSIRVQISLGKYQDQLLKLEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGID
                     EVVAESGALRLMKNQVWAYDSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDP
                     KNADLADLGTVMPHVYSQKEEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLP
                     PNFLIFDEYVVYMEMLTTKESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGI
                     RDQFNFRVALGRMSELGYSMMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKG
                     YDFLREISDIGGLNVNTEG"
     gene            1298..>1653
                     /locus_tag="AC1ZCC_002809"
     CDS             1298..>1653
                     /locus_tag="AC1ZCC_002809"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HIQ1631008.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRG"
BASE COUNT          482 a          271 c          383 g          517 t
ORIGIN      
        1 ttagacaatt tgatatttct aagctatcac tttctactat agaaatcgtt cgttctataa
       61 aagatttaat ctatttgcta ggaagtctcg ttctttcagg aagcacgatg ttgctttata
      121 tacacttttt tcaggatcat tggcgcagtt tgtggcatcg acaaaagcta gcccggatga
      181 ttctagaaaa tcattggtac gaagtaaaac aaactcagag tgaaggattt ttcaaagacc
      241 tcaatagtag tcgaaccaaa gagacaatca gttacttccc caaaatctat taccgtatga
      301 aggatggttt actttctatt cgcgttcaaa tttcactggg aaaatatcaa gatcagcttt
      361 taaagctgga aaagaagtta gaaagtggtc tatattgtga actagtagaa aaggaactca
      421 aagactccta tgtggagtac actttactgt atgatataat tgccaatcga attgggatag
      481 acgaagtagt ggcagaaagt ggggctttgc gattgatgaa aaatcaagta tgggcttatg
      541 attctttacc tcatatgtta atcgctggcg gcacaggtgg tgggaagact tactttttac
      601 tcactatcat cgaggcttta ttaaaatctg atgccgagct ttttgttctt gatcccaaaa
      661 atgcggactt agccgattta ggtacggtaa tgcctcatgt ctattcccaa aaggaggaaa
      721 tttctgcttg tgtggaagat ttctatgagc gcatgatgac tcgtagcaag gcaatgaaag
      781 aaatgcccaa ctacaaaaca ggagagaatt atgcgtttct tggacttcca ccaaactttt
      841 taatctttga tgaatacgtg gtttatatgg aaatgttaac gacgaaggaa agtgcggtga
      901 ttttgaataa gctcaaacaa atcgtgatgt taggtcgtca atctggtttc tttctgattc
      961 tagcttgtca aagaccggat gcgaaatatt taggagacgg gattcgcgat caatttaatt
     1021 tccgggtggc tttgggtcgt atgagtgaac tcggatattc catgatgttt ggggaggtcg
     1081 aaaaaaactt ctttatgaag cacatcaaag gtcgaggtta tgtggataca ggtggcagtg
     1141 tgattagtga gttttatacg ccacttgtac caaaaggata tgatttctta agggaaatta
     1201 gcgacattgg tggattaaac gttaatacgg aaggataaaa taatagtatg tagcctagga
     1261 gtgtcaagaa tcctgggctt tttttacgag gtgatttatg acatttttag atttattcgc
     1321 tgggattggt ggcttttgct taggtatgga acaagcgggt caccagtgta tcggcttttg
     1381 tgaaattgat gattttgctc gccagagtta taaggcaatc catgatacaa gtaaggaggt
     1441 ggaaatgcat gacatcacaa gtgtatcaga cgagtttatt caatctctcg gaccagtgga
     1501 tatcctttgc ggtggatttc cgtgccaagc tttttcaatt gcgggaaagc ggcaaggatt
     1561 ttccgatact cgaggtactt tattctttga aatcgctcgg ttcgccgcta ttctccaacc
     1621 taagttttta ttccttgaga acgtccgggg att
//
LOCUS       DBNICI010000253         1633 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.263, whole genome shotgun sequence.
ACCESSION   DBNICI010000253 DBNICI010000000
VERSION     DBNICI010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.263"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            146..1630
                     /gene="guaB"
                     /locus_tag="AC1ZCC_002810"
     CDS             146..1630
                     /gene="guaB"
                     /locus_tag="AC1ZCC_002810"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIQ1631009.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT          561 a          284 c          379 g          409 t
ORIGIN      
        1 acaatctctt ttaatacaca cagatgtaaa cgctttatat tggtattcta tgagaaagct
       61 aacttgattc caagtagatc attgacttaa atgaaaaaat caattatttt aatagataaa
      121 ctaaattaga ggagtggtac taaaaatgtc caactgggaa acaaagttca cgaaaaaagg
      181 atttactttt gatgatgtct tattgatacc agcagaaagt cacgtgttac caaacgaagt
      241 ggatatgagc gtacaattag ctaaaaatat taagttgaat attccaatca tcagtgcaag
      301 tatggatact gttaccgaca gcaagatggc cattgctatg gcaagacaag gcggactagg
      361 tgtgatccat aaaaacatga cgatcagcca gcaagcggat gaagtaagaa aagtcaaacg
      421 ttctgaaagt ggcgtaatca ttgatccgtt cttcttaacg ccgcagcatc ttgtagcaga
      481 cgcagaagaa ttgatgagca aatatcgtat cagtggtgtg ccaatcgttg aaacacttga
      541 aaacagaaaa ttggtaggga taattacaaa ccgcgacatg cgttttgtaa cagattatca
      601 catgccgatt gctgatgtga tgacaaaaga caacttagtg actgccccag taggtacctc
      661 tctaaaagat gcagaaaaga ttttgcaaaa acataaaatc gaaaaattac caatcgtaga
      721 taacgaaggt cgtttaagcg gattgatcac gatcaaagac atcgagaaag tgatcgaatt
      781 tccaaatgca gcaaaagatg aacatggtcg attgcttgta gcagcagccg ttggtgtaac
      841 aagcgataca tttgagcgtg cgaatgcttt attagaagca ggggcagatg caatcatcat
      901 cgatacagct catggacata gtgcaggtgt gatccgaaaa attcaagaaa tccgttcaac
      961 ttttgcagat gcaacattga ttgcaggaaa cgttgcgacg gctgaagcga caaaagcttt
     1021 atatgatgca ggtgtagatg tcgtaaaagt tggtatcgga ccaggatcga tttgtacgac
     1081 acgtgtggta gccggtgtag gtgtacctca gctcacagct atttatgatg cagcatcagt
     1141 tgctcgtcaa tatggcaaag cgattattgc agacggaggg atcaaatatt ctggtgacat
     1201 cgtcaaagca ttagctgccg gcggacatgc agtgatgcta ggaagtatgt tggcagggac
     1261 agatgaatca ccaggtgaat tcgaaatctt ccaaggacgt cgattcaaaa cataccgagg
     1321 tatgggctca ttaggcgcga tggaaaaagg ttcaaaagac cgttacttcc aaggtagtgt
     1381 caatgaagca aataaattag taccagaagg aatcgaaggt cgggttgctt ataaaggaag
     1441 tgttgcagat attattttcc aaatgatcgg cggtttgcgt tcaggaatgg gttatgttgg
     1501 ggcagccaat ctacagcaat tacgtgatga agcgcaattt atccaaatga gcggcagtgg
     1561 attaaaagaa tcacaccctc atgatgtaca aatcacaaaa gaagcaccaa actattcagt
     1621 tgaatcttaa aaa
//
LOCUS       DBNICI010000254         1583 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.264, whole genome shotgun sequence.
ACCESSION   DBNICI010000254 DBNICI010000000
VERSION     DBNICI010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.264"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            28..645
                     /locus_tag="AC1ZCC_002811"
     CDS             28..645
                     /locus_tag="AC1ZCC_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631010.1"
                     /translation="MWIFKQEIFAKLFSLDGLGWGILFFIFLFLGSMWIWFVDKKNSY
                     EKVIVKKMNRNEFDDFSKEHRVELYDSHEINSTSPSYYWVFGCEEKPLEIKLLKMLEN
                     PPVIEDFSAKNVEKQMFEQVQIRGQNKISLEPDEYAAFFLETNSGTSMPVFKVKLKMN
                     GRLCTYDLVANMRYGSTGDRRTILKFEQTFRSFLKNRVSQIFNLD"
     gene            809..1141
                     /locus_tag="AC1ZCC_002812"
     CDS             809..1141
                     /locus_tag="AC1ZCC_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631011.1"
                     /translation="MNNREILAKEMADLKAKEAKIRAKRKQIRQKEMISLAKLYCKKE
                     GFKTPAEALDFLESNKRNHSVNDEVLVSLRKFADGLVWQENGAYWRTNDVKGLTQFMA
                     QFRSENVN"
     gene            1474..>1583
                     /locus_tag="AC1ZCC_002813"
     CDS             1474..>1583
                     /locus_tag="AC1ZCC_002813"
                     /inference="COORDINATES: protein motif:HMM:NF013258.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HIQ1631012.1"
                     /translation="MVARTQKHKVGSLNGLQNHVDRKTKNHTNKDIDITK"
BASE COUNT          570 a          186 c          313 g          514 t
ORIGIN      
        1 ttattttaaa gtgggaggaa aattgttttg tggatattta aacaagaaat ctttgctaaa
       61 ttatttagtt tagatggatt aggttgggga atcttatttt ttattttttt atttttagga
      121 tctatgtgga tctggtttgt tgataaaaaa aattcttatg agaaagtaat agtgaaaaaa
      181 atgaatcgaa atgaattcga tgatttttca aaggaacata gagttgaatt gtacgatagt
      241 catgaaatta acagtacttc tccatcttat tattgggtat ttggttgcga agaaaaacca
      301 ttggagatta aattgttgaa aatgcttgaa aatcctccag tcattgaaga tttttctgca
      361 aaaaacgttg aaaaacagat gtttgaacag gtacaaataa gaggtcaaaa caaaatttca
      421 ttagaaccag atgaatatgc agcctttttt ttagaaacaa attcaggtac ttcaatgcct
      481 gttttcaaag ttaaattaaa aatgaatggt cgtttatgca cttatgatct tgttgcaaat
      541 atgcgctatg gttctactgg tgatagacgt actattttaa aatttgagca aacctttaga
      601 agttttttga aaaatagagt atcccaaatt tttaatttgg attgaaaaaa ttaaaaattt
      661 ggccaaaaat actacagttt tttagagcaa tcatcttatg atgattgctc tttttttgtt
      721 gtttttgtgt cattcgttgt tcaaatcctc tattcgtaaa ataaatattt taaactagaa
      781 atatgcctta tttttaggag gaatcaaaat gaacaataga gaaatattgg caaaagaaat
      841 ggcggatttg aaagcaaaag aagcgaagat tagagcgaaa cgaaaacaga ttagacagaa
      901 agaaatgatt tctttagcga aattatattg taaaaaggaa ggctttaaga cacctgctga
      961 agcattagat tttttggaat caaataaaag aaatcattca gtaaatgatg aggttttagt
     1021 atcgctaaga aaatttgcag atggattagt ttggcaagaa aatggtgcat attggcgtac
     1081 gaatgatgtt aagggtttga ctcaatttat ggcacaattt cgttcagaaa atgtgaacta
     1141 aattgaggag gggttgaagt gaaaagcaat acttttcact tttggttttt tgcggaataa
     1201 aaattgtgaa aacaatgacg caaaaagcca atggttttga acgaaaaaag gacgtaaaaa
     1261 aggaatgcga aggcagtcgt ttttcgttcg ctttttcgtt tgagagaaac ggagtttctc
     1321 atcggctgtt aagccgatat agccctcggc agagcttaca gactttacgc agtataagtc
     1381 tagtaagtta taccttacat ggaggttttt ccgaatttag cttgtgattc tcgcaataat
     1441 tttaacgaaa ggaagtacac agttatggcc tatatggtgg cacgaacaca aaagcataaa
     1501 gttgggtcgt taaatggact tcaaaatcat gtagatcgta aaacgaaaaa tcatacgaat
     1561 aaagacattg atattacgaa aag
//
LOCUS       DBNICI010000255         1581 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.265, whole genome shotgun sequence.
ACCESSION   DBNICI010000255 DBNICI010000000
VERSION     DBNICI010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.265"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..132)
                     /locus_tag="AC1ZCC_002814"
     CDS             complement(<1..132)
                     /locus_tag="AC1ZCC_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HIQ1631013.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..1516
                     /gene="bsh"
                     /locus_tag="AC1ZCC_002815"
     CDS             542..1516
                     /gene="bsh"
                     /locus_tag="AC1ZCC_002815"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HIQ1631014.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          570 a          227 c          280 g          504 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaataaac
      541 tatgtgtacg tctattactt atgtaacaag tgatcattat tttggaagga attttgatta
      601 tgaaatatct tacaatgaag tagttactgt tactccaaga aattataagt tgaattttcg
      661 aaaggtaaat gatttggata ctcattatgc aatgattggt attgccgctg gtatagctga
      721 ctaccctctt tattacgatg cgacaaatga aaaaggattg agtatggctg ggctaaattt
      781 ttctgggtat gctgattata aagaaataca agaagggaaa gacaatgtat ctccttttga
      841 atttattcct tggattttag gacaatgctc aacagtagga gaagctaaaa aattgttaaa
      901 aaatatcaat ttagcaaata taaattatag tgacgaactt cctttatccc ctttacattg
      961 gctattagct gataaagaaa aatcaattgt cattgaaagt atgaaagatg gacttcatat
     1021 atatgataac cctgtgggcg ttcttaccaa taatccttca tttgactatc aattatttaa
     1081 tttaaacaat tatcgtgtct tatcgagtga aactcctaaa aataattttt caaatcaaat
     1141 aagtttgaat gcctatagcc gcggtatggg agggataggc ttgcctggag atttatcctc
     1201 agtatctcgt tttgttaaag cgacttttac gaagctgaat tctgtatctg gagattcaga
     1261 gtcagaaagt attagtcaat ttttccatat cttaggttca gtagaacaac aaaaaggttt
     1321 gtgtgatgta ggtgatggaa aatatgaata tacaatttat tcttcttgtt gcaatgttga
     1381 caaaggaatc tattattatc gaacatatga agacagtcaa attactgcaa ttgatatgaa
     1441 taaagaagac ttagatagtc ataagttaat tagttatcca attatagaaa aacaacaaat
     1501 taaatatata aattagttaa tgtgttgtga ttgattattt aatatagata taatacaaaa
     1561 aggcaaagat attttcaaaa c
//
LOCUS       DBNICI010000256         1538 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.266, whole genome shotgun sequence.
ACCESSION   DBNICI010000256 DBNICI010000000
VERSION     DBNICI010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.266"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..543
                     /locus_tag="AC1ZCC_002816"
     CDS             <1..543
                     /locus_tag="AC1ZCC_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HIQ1631015.1"
                     /translation="LCQVQHYMNVLNKDYCYIAVLIGGQKFIWKRIERDQELIDTITE
                     QLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTTLPIHFDELVEQKNELKR
                     TKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVRTRRMNSKKLAEKYPDVA
                     NDEEIYNVTESRRLTEREIK"
     gene            545..1435
                     /locus_tag="AC1ZCC_002817"
     CDS             545..1435
                     /locus_tag="AC1ZCC_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HIQ1631016.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
BASE COUNT          568 a          248 c          333 g          389 t
ORIGIN      
        1 ctctgtcaag ttcagcatta catgaacgtt ctaaacaaag actattgtta catcgctgtc
       61 ttaatcggtg gtcaaaaatt tatctggaag cggattgaac gagatcaaga gctgatcgat
      121 acaatcactg aacaattagt agagttttgg gaaacaaacg ttcttggagg tatcgagcca
      181 attattgatg gtagtcaagc gacagctgat ttcttgaaag agaagtatgc agatgttgaa
      241 gacgttcaaa cgacgttacc aattcatttc gatgaactag ttgaacagaa aaacgaactc
      301 aaacggacta agaaagaaat tgaatcagct atccgtcaag tagacaacga gattatcagc
      361 gagctaggta aacgtgaagc cagtatcggt atcactcaaa aaaacatcat cagttggaaa
      421 tttgtccgta cgagacgtat gaactcgaag aaactagcag agaaatatcc agatgtcgca
      481 aatgatgaag agatttataa cgttactgaa tcaagaaggc taaccgaaag ggagatcaaa
      541 taatatggca acaaatgaat cgttaaaaaa tcaattggca gcaaagccac agaaacaagt
      601 tgcaccaggt caattaggac ttaaagcttt gatgaataca ccaacaatga gaaagaagtt
      661 cgaagaggtg cttcatgaca atgctaatgc ttttatgtcg aatgttatga ctcttgtatc
      721 taatgacagt tatcttgcag atagtgaacc gatgtctatc atgagtggtg cgttaactgc
      781 tgcaacatta aatcttgggc tagataagaa tttaggttat gcatatttag ttccattcaa
      841 tagtaaaaac aagcaaacag gaaaatggga aaagaaagct caattcatgc ttggctataa
      901 aggatatatc caattagccc aacgatcagg taaatacaaa gcattaaatg tgatcgaagt
      961 ttacgaagga gaactaaaaa gctggaaccg actgacagaa gagtttgagt ttgatccaaa
     1021 tggtagaacg tctgatgaag tcattggata tgttggctat ttcgagttac tgaatggatt
     1081 caaaaaaact gtctattgga ccaaacaaga aatcgaagct catcggattg ctaacaataa
     1141 agatcgcgat aagacaaagt taagtggtgt gtgggcatct gattacaatg caatggcacg
     1201 aaaaactgtt ttgagaaatc ttctttctaa atggggaatc ttgtccatcg aaatgcaaga
     1261 agccaccaca tcggatgaga gagtccaaag ggttcaagaa gacggcagca ttattgctga
     1321 aacagaagtt gaagaagata ttcctgaaag aaaagaagca gaggttatat ctgaagaaaa
     1381 tgaagatgta caaactggat tatttgatgc atctaatccg ccgttaaaca aataatgagg
     1441 gagctttctc ccttgctttt ctagaggaga aatacgaatg aacacaggat atgtcaaatt
     1501 acatcgaaaa gtgatgaatt cattcgtttg gaccaatc
//
LOCUS       DBNICI010000257         1521 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.267, whole genome shotgun sequence.
ACCESSION   DBNICI010000257 DBNICI010000000
VERSION     DBNICI010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.267"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(55..399)
                     /locus_tag="AC1ZCC_002818"
     CDS             complement(55..399)
                     /locus_tag="AC1ZCC_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIQ1631017.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            complement(425..592)
                     /locus_tag="AC1ZCC_002819"
     CDS             complement(425..592)
                     /locus_tag="AC1ZCC_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631018.1"
                     /translation="MYRPQYLEQKHEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKY"
     gene            complement(659..1306)
                     /locus_tag="AC1ZCC_002820"
     CDS             complement(659..1306)
                     /locus_tag="AC1ZCC_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631019.1"
                     /translation="MVGVVKFLGFTFDGYGTLGEWVGIILIVITIQQTSKYYWYSNSS
                     KIVSRITEGITKKDITVVGKYGFTITLLNEGKSETAIKFCGIIGKYNIFQRFIRKHWY
                     KYIYKYPVVKKLIEKYVDHEISYLNPTDLINQEFQNIGYLEKGLKIPIVNESLVTAMV
                     ANIKNSKKLEKRYKKCKKLKFSFLFLEYNGKEYYQDIYVLRGSNLYKDIKNKISV"
BASE COUNT          445 a          304 c          188 g          584 t
ORIGIN      
        1 tcttcaatct tttctgtatc atactctctg ccattgtcat tgatgatcat gcacttacca
       61 cctttcactt gcatcgaagt tagcgaagct ttctatcttc ttctcttgtt tatctaacgc
      121 tgtaagatat ctgccatgaa cttcattatg atgttcaaca cataaacaaa taagattatc
      181 taaatctaaa gctaagtcag gtctatcctt gacttccttt atatgatgaa cgttctctac
      241 tctatgatac ttacctagtc ttctacactc ttggcattca tagtgatctc gtttcatcgc
      301 tttctctcta agcctgcgcc atttaggaga ctgatagaac ttaaccaaac gatcttctct
      361 tatcaactgt aataaccatc tatagaattc ctcggtcatg tcccatctcc tttcgcaatc
      421 ttatttaata cttagctatt cttttgccat acaatggaat aacttcattg ctttccttcc
      481 gtttatatgt atcgctcttt attggtcttc tatactttcg tactatctca ccgttaccgt
      541 tttgcacagt gattacttca tgcttctgtt ctaagtattg tggtctatac atcgtcgtgc
      601 ctcctttacg caaaataaaa agaccactca aagagtgatc tacgaatttt aaaaaatctc
      661 atactgaaat cttattttta atgtctttat ataagttcga gcctcgcaaa acataaatat
      721 cttgataata ttctttgcca ttatattcta agaataaaaa ggaaaatttc aattttttac
      781 attttttata tctcttttct aatttcttgc tgttctttat gttagctacc attgcagtaa
      841 ctaaactttc atttacaatt ggtattttta gacctttttc taagtatcca atattctgga
      901 attcttgatt aataaggtct gttggattga gataagatat ttcgtggtcc acatattttt
      961 caataagctt tttaactaca gggtatttgt aaatatattt gtaccaatgc tttctgataa
     1021 aacgttggaa aatgttatat ttacctataa tcccgcaaaa ctttattgcg gtttcagatt
     1081 tcccttcatt taataaagtt atagtaaacc catatttacc gacgacagta atatcttttt
     1141 tggttattcc ttcagttatt cttgatacaa ttttagaact attagaatac cagtagtact
     1201 ttgatgtttg ttgaattgta atcactatca aaataatacc tacccattcg cctaacgtac
     1261 catatccatc aaatgtaaac ccaagaaatt taactacgcc caccattttc tatcacctca
     1321 gaaataatat tatcaaataa tcttagataa ctaaacaggg gacttgtaaa tttcagataa
     1381 ttactcattg acttttccca ttcatccttt attgcgtcta ccctttccgc cacagcgaca
     1441 aattgatatt gtgaaatttt atacatagct tataatttta attatcagcg agtggtccgc
     1501 tgaaataaat tataggtggt g
//
LOCUS       DBNICI010000258         1476 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.268, whole genome shotgun sequence.
ACCESSION   DBNICI010000258 DBNICI010000000
VERSION     DBNICI010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.268"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            100..1392
                     /locus_tag="AC1ZCC_002821"
     CDS             100..1392
                     /locus_tag="AC1ZCC_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1251 family transposase"
                     /protein_id="HIQ1631020.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          478 a          299 c          267 g          432 t
ORIGIN      
        1 tgacaccagc atctttttta ttttccaaaa catatagaaa agacaccttg aaatccttta
       61 gaattaattt ggcgaaccaa cccaaaagga ggaatcaaga tgtcttacac tcaccttatc
      121 aaagaaacat tagatattct agacttatca gttactttta acgaaaattg tctcacaaaa
      181 gaaaaataca aagggcaaat ctgtcatatt tatcgtggaa acttgattta tacagcccaa
      241 gaatgcatcc actgtaaaca ccagattgct tcagatattg tgcgttgggg tacaacaacc
      301 gtccggttgt taatgaacga tgtttctgaa taccgtacct acctcgaatt gaaaaagcaa
      361 cgcttcaaat gcaaagcttg tcagcggacc tttgtcgcag atacttctgt tgcgaaaaaa
      421 cattgtttca tcagtgagaa agtccgctgg tctgtcgtga ctcgtctgaa aaaaaatacc
      481 tcaatgacag agatcgccgc tcagaaaaat ctatcggtct cttctgttta ccgtattatg
      541 aagcgatttt atcgtccgtt gaatcctttt cggacaccgt tacctaaggt actttgtttt
      601 gatgaattca aatcagttcg gggtgtttca ggtgctatga gtttcatcat gatggatgga
      661 caaacacagc gcttattgga cattgtcgag aaccgacaac tgcctttttt aaaacgttat
      721 tttagtcatt tttcacgaga aattcgtgaa gcggtcgaat ggatcgtgat tgatatgtac
      781 gctccttatg tatcgctcgt taaaaagctg tttccaaaag cacagctgat tattgaccgg
      841 tttcacatcg tccaacatat tggtcgtacc tttcgcaatc atcgaatcaa agaaacgaac
      901 caactattaa aaagcaaaga acaaaaacac tatcaattag gtaagcaatt aaaaagatac
      961 tggaaacttc ttcaaaaaga tgaacgcaag cttgattata ctcgacgcct ttggcgacct
     1021 ggcttcaaag ctcatttaac agagaccgat attgtggatc gcttactgaa agggagccct
     1081 gctttgcgtg taggctatca gttatatcaa gactttcttt acgctgtaaa ggaacgagac
     1141 tacgtatcgt ttgaagaact acttaccaat aatatcatgc ttcccgaagg ctatcagacg
     1201 accttgagaa cgttccagaa atttctgcca caaatcaaaa atgctttaca acaaagttat
     1261 tcaaatggcc cacttgaatg tctgaacaac catatcaaag tgttaaaacg aaatgcatat
     1321 ggctttcgta gcttttataa tttcaaattg cgcatcatga ttcgtcatgg caacgcattg
     1381 attttcaact aaaaaagatc aagaggaact gatccacttg atctccaatt attctggctc
     1441 tataatttat gggactgatg aatcagaatt gattct
//
LOCUS       DBNICI010000259         1465 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.269, whole genome shotgun sequence.
ACCESSION   DBNICI010000259 DBNICI010000000
VERSION     DBNICI010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.269"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
BASE COUNT          556 a          165 c          299 g          445 t
ORIGIN      
        1 ttaatttgac aattaagaaa gtattaactt gttttgtgaa aaagagaatt tgaaaaacga
       61 ttggcgaaca aatgctcggt aggtatggta taaatacaga gctaattggt gtgatgaaat
      121 cagatattcg cgtgagattc agtaaagtag gagttcgaaa acagctaggt gacaaacagt
      181 attgctttgg tacttcggaa tggtttatca aattcttatc aacagttcac ttctctttat
      241 gtttgagtga tgattcgtta ggttattgct tggaaagacc ggatgagagt agaagagttt
      301 aattttataa agtgaaaaga ggtaatgtac gatatacgta gggctgaacc aatagaggat
      361 aagcaaacga tgttacaggc gcaaaattca tagcttactg ttttggagat aacagaagag
      421 gaaataatgc agaagtaagg cgaaaaaagg acggaagcct aacagcatat gaaataaaga
      481 aaaatattat aactactgat aaatcggtat tgagcaaagt actaatagga gtcaaaaaca
      541 ttcataagct atgagtgttt cttggctact tttttatttt tatgtatcta caaaagtttc
      601 caggggcaga acctgtgatt attaatggaa gaacctcaag tattgtctta aatcattgtt
      661 atgaaaatgg attaaaagag ttcatcaagg tgtgattaac aagttacttt tcattacatc
      721 gaatttttca gaagttgctt atattggtga tgatattaat gatttatcat gtatgacatc
      781 tgtgaagaag cgggaggaat agtagaatgt ccaaaaaatg ccacaagaaa tgagattgaa
      841 ttatctgatt ttattgtaac tcacagtggt ggtgacgaat ctgtgataga gtttattgag
      901 tggatattgg aatcgatata gtgtagtagt acagaaaaat tatcatacaa ttaatactag
      961 gcataagaat attggcatat tctagagtta aaagtaacta ttcgaaaagg ataaggttag
     1021 aattttttgt tttgccagtg gaagaatata aaaagggcat tggaaattaa tatattagaa
     1081 tgctaagcgg agaaaaaaat atatgaaaag tacgtccttg tgatgggatg gaaaaataca
     1141 cttatttcaa acaacgataa tataattatg tgaaccgaag tatactcgct tctaattcaa
     1201 tcctcaaatc ccgacagtcg cgacttcaaa aaagcgtata gttttaatgt atccccatat
     1261 gatccgaaaa atggtaattt acaattaaca gtttgatgat gttgaagatg tggaaatgct
     1321 gaaattacca tgatgagaaa cacattaatt aaagggaatt aattaactag aatataatct
     1381 gattcaattt gaattaagca aaaaaaggct gagttttaaa taatcgcgaa ttgtaaaatt
     1441 aagttagaaa aataaaaagg cattt
//
LOCUS       DBNICI010000260         1447 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.270, whole genome shotgun sequence.
ACCESSION   DBNICI010000260 DBNICI010000000
VERSION     DBNICI010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.270"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            96..1391
                     /locus_tag="AC1ZCC_002822"
     CDS             96..1391
                     /locus_tag="AC1ZCC_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HIQ1631021.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          457 a          286 c          268 g          436 t
ORIGIN      
        1 cttcaccaac atcttttttg tataaaaaaa tatagaaaag acaccctgaa atcctttaga
       61 attaatttgg ctaccaattc ataggaggat tcaagatgtc tcatactcag cttatcaaag
      121 ataccctaaa tattctagac ctaaatatct atttcgagga aaattgttta actaaagaaa
      181 aatacaaagg gcaaatttgc ttgatttaca aaggaactct tctgtacaag ccagaagagt
      241 gttcccattg tctttgtgtc gtcccctcta ggatcattcg ttgggggacg acaaccgttc
      301 ggttgttact gaatgatgtg tctgagtatc gcacttatct ggagctgaag aaacaacgat
      361 ttaaatgtaa gtcttgccaa agaacattcg tagcggatac ctctgttgct gaaaaacact
      421 gtttcattag tcaaaaagtt cgttggtctg tcattgctcg cttaaaagag aatacttcca
      481 tgacggaaat cgctcgtcag aaaaatattt ccacttcttc ggtctatcgt gtcatgaagc
      541 gattctaccg cccattgaat cctttcaaac agactttgcc aaaggtctta tgctttgatg
      601 aattcaaatc ggttcgaagt gttgccagtg caatgagttt tatcatgatg gacggacaaa
      661 gccatgcatt attggatatt gtcgaaaatc gacgcttgcc ttatttagaa cgctattttt
      721 ctcggttttc attagcgact cgtgaagccg ttcaattgat tgttattgat atgtatgcgc
      781 cttatgtttc gttggtgaaa aagcttttcc cgaatgctca gttgatcatc gatcgtttcc
      841 acatcgtcca acatattggc agaactttct tgaatcatcg agtgaaagaa accacggtac
      901 gacttaaagg aaattcccac tctcaacgag ggttagggaa aaaattgaag cgctattgga
      961 aactcctaca aaaagaagaa gcaaagttaa actatgaaaa acgggtttgg cgtgccagtt
     1021 ttaaagatta tttgactgaa agtgagattg ccgatcgact gttagctgct tgtcccaact
     1081 tacgccaagg ctatcagtta taccaagaca ttctttatgc ggtaaagaaa agagatcagt
     1141 cactatttga ggattgttta actagagaca ttagcggtct gccagaaacc tatacaacca
     1201 ctttacgaac gtttaaaaag tttctgccac agatccaaaa tgcgcttcac tacagctatt
     1261 caaacggacc attggaatgc ttaaacaacc acatcaaagt attaaaacgc aatgcgtacg
     1321 gctttcgcaa cttttacaat ttcaaactac gaattatgat tcgtcatggc aaaacatttt
     1381 taaccaaata aacagaacag aaagaggttt tcttctttct gttctgtcca aattattcta
     1441 agatttc
//
LOCUS       DBNICI010000261         1381 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.271, whole genome shotgun sequence.
ACCESSION   DBNICI010000261 DBNICI010000000
VERSION     DBNICI010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.271"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(20..1207)
                     /locus_tag="AC1ZCC_002823"
     CDS             complement(20..1207)
                     /locus_tag="AC1ZCC_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HIQ1631022.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          410 a          292 c          224 g          455 t
ORIGIN      
        1 ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat tttcgtggag
       61 aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga gtgatggaat
      121 tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg ttcaaccgtt
      181 ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac aaaatttgaa
      241 tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt gaatcacaac
      301 catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca gaggctttaa
      361 agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc ttatcaaaaa
      421 tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag ttcttgcgaa
      481 cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg aacaaacctc
      541 gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg tcttgaacat
      601 caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata tatacacctt
      661 ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac atgaaaaatg
      721 gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga tccaactgtt
      781 tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc agttcaactg
      841 ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg gcagcgatca
      901 aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta ccgtctctgg
      961 ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga tcgtagtaac
     1021 catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga atatattgat
     1081 ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt gcggtatcat
     1141 tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta aaatgtactt
     1201 gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg tactcctaac
     1261 tgagatcaat ggcttttttg atgcttcact tttacacaat tatatggacg taatccaata
     1321 aaaaatcatc aaataatcct ttacacgtta ttctaaattt aatttttatt attttattga
     1381 t
//
LOCUS       DBNICI010000262         1355 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.272, whole genome shotgun sequence.
ACCESSION   DBNICI010000262 DBNICI010000000
VERSION     DBNICI010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.272"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..1355
                     /locus_tag="AC1ZCC_002824"
     rRNA            <1..1355
                     /locus_tag="AC1ZCC_002824"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DBNICI010000263         1307 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.274, whole genome shotgun sequence.
ACCESSION   DBNICI010000263 DBNICI010000000
VERSION     DBNICI010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.274"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..878)
                     /locus_tag="AC1ZCC_002825"
     CDS             complement(<1..878)
                     /locus_tag="AC1ZCC_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HIQ1631023.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKN"
     gene            complement(884..>1307)
                     /locus_tag="AC1ZCC_002826"
     CDS             complement(884..>1307)
                     /locus_tag="AC1ZCC_002826"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIQ1631024.1"
                     /translation="IGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLML
                     PSLFEGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDI
                     SHRDSERSILIKADYNIQTAYKILESKYYELFELIQRG"
BASE COUNT          402 a          243 c          151 g          511 t
ORIGIN      
        1 aaatttttaa tcaattcgtc acataatgga ttgttactag gtattttatc aaatgtctgt
       61 gctatttcat gatttattct ctgtgctaaa acaatcatat aatctatcat caaataatcc
      121 accatcatat cattattttt ccaataattt aaaaaaaagt ctctaattat agcaaacatc
      181 catctagact cttgattaca tgctaatgaa taacccgcaa aatatcctga tgctacggat
      241 gcgtgagcat aatcaggcct cctaatagac caaatatcga tatcaaaata ctcatctggt
      301 attggttttg tacaataaaa tgtggcgtct aaccacaaac ctccatgttt tgctagcaaa
      361 gataatctta ataaatcaga ataatttgtt ctcgtaatga ttcctttgtt tttcttttct
      421 tccacccatt ttggtatatc aacatattgt ctataattat cttctgttaa aattataaca
      481 gtatgtttcc ctgcatttcg ttttattgat tttacacaag cttttacaat ttccggcgat
      541 tgatccaatc cctgccacca acaaacccaa atgcagttag aatatggtga ttttgattga
      601 ttaattattt gattttgact ataatcatat gttttcaaaa aatctgaaaa agtcttttca
      661 taatacttca gcattacctc atgttttttt aataaatgtt ccttctgttt tttactttca
      721 ttatatccat tacgattcat gacttgaata tcaattttag cttgtaacgt ggcaaatgct
      781 tccttccaag atgttatctt tgcaattcgt cttgttgcat tcaattcttc ggatatgcga
      841 tgtataattc tttttatact tgaatgtttc tgtgtcatat tctttaacct ctttgtatta
      901 attcaaataa ctcataatat tttgattcta agattttgta tgctgtttga atattataat
      961 ctgcctttat taaaatcgaa cgttctgaat ctcgatgaga gatatctaca tttgcaatct
     1021 ctctcgccca ttcttttgcc gattgattta aacttatgaa attggtctta tcactgaaag
     1081 caacttctgt aggaactttc tccgaaaaga aacacggtaa atctgcaaat tgagcttcaa
     1141 tacccactaa cggaacccct tcaaataatg atggcaacat taatacgtca aatgcttgat
     1201 aaaatttttc tatatcattt ctatttccta aaaatttaat atcatcatct atgccacttt
     1261 tctttgcata attttttatt tcttcttcca tatcaccagt tccaata
//
LOCUS       DBNICI010000264         1299 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.275, whole genome shotgun sequence.
ACCESSION   DBNICI010000264 DBNICI010000000
VERSION     DBNICI010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.275"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            143..400
                     /locus_tag="AC1ZCC_002827"
     CDS             143..400
                     /locus_tag="AC1ZCC_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631025.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(632..1063)
                     /locus_tag="AC1ZCC_002828"
     CDS             complement(632..1063)
                     /locus_tag="AC1ZCC_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HIQ1631026.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          490 a          165 c          162 g          482 t
ORIGIN      
        1 ttttctgtcc acgttcattt tactccaaag ttttcctcat ttcaaaagaa aatcacagaa
       61 tacacgaaga agcgtttcta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa tttagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaattaaa aaagttcttc atgaagctga taaatatgaa aaatatgatg aaaaagaact
      361 tgataaagaa tttaaagaaa tcaatgactt attactataa ttgatttata cgactcattt
      421 ttgagtcgtc agactgcaaa caacactcta aatttaggag tttgtctaca gtctgaatag
      481 attttaaatt tattgtccat aatgttatta tgaatttata gcattaagaa aagagataag
      541 actttacatt tttttaggaa tgtaaagtct tatctctttt cttaattgta caattagtat
      601 tatctgcact actttctaat taaataaatt tttagttatt aacttgatta aaatcagaga
      661 taatatcatt taattcagtt tgaagggttg taacgattaa atggaaattt tcttcatctg
      721 ttttattata tggggatgtt ttcgttatat ataacaactt ttctttttca attttcatta
      781 ataactctgt cgaagtctgt actttatctt gataattaaa tgcagacata ccactttgta
      841 taattgtaat tattgctgaa gcaattgaaa caatcaatag taaagacctg tgttcggaag
      901 cctgatcaat taatattggg atactagctg ataagactat ttttataata tttccgatac
      961 caattactct attataaaat cgaatttctt tttttaatct atcgattgta gaatcaatag
     1021 attcgataaa cgctttttga tccatcattt tctctccaat cattttatta aaatcattaa
     1081 caaaaatatt ataataggat ttaagtaagt tctaactaat cacatataaa aataaaaaaa
     1141 catttgtatt ttatcaaagt tttattatac ttgtcaattt gtgtacatcc taaaatataa
     1201 gctatttgtt tacttctgta gaatatatac tttgttgtta gataagttgc acatgtttat
     1261 agaatctaaa taataaattt ttatgtaccg cccctcaaa
//
LOCUS       DBNICI010000265         1276 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.276, whole genome shotgun sequence.
ACCESSION   DBNICI010000265 DBNICI010000000
VERSION     DBNICI010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.276"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            35..532
                     /locus_tag="AC1ZCC_002829"
     CDS             35..532
                     /locus_tag="AC1ZCC_002829"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1631027.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(569..1252)
                     /locus_tag="AC1ZCC_002830"
     CDS             complement(569..1252)
                     /locus_tag="AC1ZCC_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIQ1631028.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
BASE COUNT          444 a          216 c          224 g          392 t
ORIGIN      
        1 atttatagta tactatgtca aaaggaggag ttagatgaac aatcaaatca cactagcgta
       61 tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt tttatgaatc
      121 gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac aattgacaac
      181 tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta ttaaggatca
      241 tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag agattcaacg
      301 gctctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac tcgatgaagc
      361 gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt atgaaaaaaa
      421 tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga tcaaacattt
      481 tcctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct aaaaaaacgt
      541 cctcactttt gatgctgagg acgttttttt agactggtaa tggaaccata attcgctttc
      601 ctttttgata aatcgctaat tccgtcacac catttgcttt taacagagca aaaagatcat
      661 cgaaatggaa acggaaatgt tcaaggtaat gcccatctga tccaacacta aacaaggtac
      721 caccacgtga aagatataaa tcgatgacat attcatataa cgctaaattg tcatacaagt
      781 aagcgctctt agcatttaat tcgaatgcca atttattttt gattacttga tctagaatcg
      841 gtaaaaactg cgcttcatat tgtttaaatt cagcaacgga tagaccaaat acccgaaaac
      901 catagtcaaa atgagcaaag acatctgctt ctataacaac tagtgcttct gaaagttgtg
      961 caaaatactg aggaatcaca atagcaggat ccatatcttt gacttcgtca tctaaataat
     1021 caaattgccc attatgatgc acactgagta attttaaatc gtaatcttta tcggctaaat
     1081 agtcgatgat ccgatccttt tcagaagcga tgtaaccgat tttgatgcct tttaaaacac
     1141 gattgccaaa acgcttattt aagctatcga ttgtttgact gtacatttca taatcgggga
     1201 tatcatcgac tttagtaaca ggattggcta agtcaaaatg ttcagttgtc acgacgtaac
     1261 catcgtagtt tgttaa
//
LOCUS       DBNICI010000266         1255 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.277, whole genome shotgun sequence.
ACCESSION   DBNICI010000266 DBNICI010000000
VERSION     DBNICI010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.277"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(22..900)
                     /locus_tag="AC1ZCC_002831"
     CDS             complement(22..900)
                     /locus_tag="AC1ZCC_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HIQ1631029.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT          393 a          233 c          196 g          433 t
ORIGIN      
        1 aactatttcg aagtactaaa cttatgaagt aagaatacct aagccagagc caatgtgctc
       61 gcctgcaagc aattgctctt ttgcttgtcg ctgtaaactc gtactttctg gataatcatg
      121 tatttgggca tctactcgtc caaagatcca agggactcca ctatcgtcct gttcatcaac
      181 tgataataat cgataatcgg ctgcaaaaag acagattttt gactcttttt tcaaccattt
      241 agtaagattg ggtgatagta accaagtatc tgtgtacatc acttggtaat ggtaatcagg
      301 aaaataaagt tcaaaaaaac gaatggcact ttcgtaacta tctcttatct tattctgaga
      361 taaatctgca tcggaaggaa tatgaagaga aaggcactgc tttccttcta atcttagatc
      421 cgtgtaccct ttcggaaaat atgtcatttc gtactccaat tcaccaatcc gaaagatcaa
      481 gccactagtg taatgccaaa tccaaaaacc acggtcaaag cgatagcttc ctgttacttg
      541 gaaatgttca tgaataaatc tagtaaaagc attcattgtt gccaaataga tagttcttgg
      601 aatcttcaat ggttgataaa tttttttcca actttgagaa gctgcatata gataaatagc
      661 taatgctgca aaaggttcct cttcatagac tttgtctatt ttctttgcag tcgcctttgc
      721 tttattagga tccattagtt catgactata ttgctcaaac acctcatcaa ggatgttttt
      781 tgcttgtaca atcatttgct ctataacgac tttgggaaga gttaaagttt ctgccagaat
      841 ttggatttca ttgcttattt tcttcatcaa atttcccctt ttcatatctt tgaacctcat
      901 tgtttaaatg actgattatc cggacaccat atggtataaa catactttta ttgtacacta
      961 actataaaac ttaaactttc tatgaagaaa gaatatctgt aagactttca aggaaagcat
     1021 tgaccatctc aggaaagttt attcagttac catcataaac aaaatttttt gatagaataa
     1081 atataaaatt tttttgaaag ttcctttcca catttttgtg tgaatttgac ttaatgctga
     1141 gaatctggct tttgaacacc ttgaattaga ggttgtgaca aaaatcttgt cataacctct
     1201 tttggcacat atatttgtcc ttctattcta atttttgacg aacaaccgtt aaata
//
LOCUS       DBNICI010000267         1247 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.278, whole genome shotgun sequence.
ACCESSION   DBNICI010000267 DBNICI010000000
VERSION     DBNICI010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.278"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            200..742
                     /locus_tag="AC1ZCC_002832"
     CDS             200..742
                     /locus_tag="AC1ZCC_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631030.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1247
                     /locus_tag="AC1ZCC_002833"
     CDS             898..>1247
                     /locus_tag="AC1ZCC_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HIQ1631031.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          515 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccaga
//
LOCUS       DBNICI010000268         1220 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.279, whole genome shotgun sequence.
ACCESSION   DBNICI010000268 DBNICI010000000
VERSION     DBNICI010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.279"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..1175
                     /locus_tag="AC1ZCC_002834"
     CDS             <1..1175
                     /locus_tag="AC1ZCC_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIQ1631032.1"
                     /translation="KHLTKKGEEKYQFSLYLGVDPLTGKKRKTTRRGFSTPLAAERAL
                     KRLEADIQEKGLQASTAQKSKKFEYVYGLWFENYKKTVKESTWSSTKQIFDTHILKVF
                     GDKFIDKIDVFFCQEAVNTWSDSHPKIFKKIKNYTSNVFDYAASLQIITSNPMKLVSI
                     PRGEALDIEDKNIEFYTKEELIKFLEAIRNDDDERYLFFSLLAFTGIRKGEAFALTWS
                     DIDFKSKTLNINKTVTRGYQGRLIVNTPKSKSGKRKIYLDNDLISLLRKYYTKNKTIV
                     TIQRENLIFHHDGLLYNPTVSRSWLNVIYKHHPELTKRITTHGFRHTHASLLFESGAS
                     LKDVQERLGHADIQTTSNIYTHVTETQNKKVINNFVAFMKNSTQGESKGESKNILG"
BASE COUNT          476 a          196 c          204 g          344 t
ORIGIN      
        1 aaaagcactt aacaaaaaaa ggcgaagaaa aatatcagtt tagtctgtac ttaggagttg
       61 atcctctaac aggaaaaaaa agaaaaacaa cgagacgagg ttttagtacg ccattagccg
      121 ctgagagagc tttaaaaaga ttggaggcag atatacagga aaaaggactg caagcttcta
      181 cagctcaaaa gagcaagaaa ttcgaatatg tttacggctt atggtttgaa aattataaaa
      241 agacagttaa agaaagcact tggtcctcca ccaaacaaat ttttgacaca cacatcttaa
      301 aagtgtttgg agataagttc attgacaaaa tagacgtttt tttctgtcaa gaagcagtaa
      361 acacttggtc cgatagccat cctaaaattt ttaagaaaat aaaaaattac acatctaatg
      421 tttttgatta cgctgcttct ttacaaatta taactagcaa tcctatgaaa ttagtttcta
      481 ttccacgtgg agaagctcta gatatcgaag ataaaaatat cgaattttat actaaagaag
      541 aattaatcaa atttttagaa gcaataagaa acgatgatga tgaaagatat ttattctttt
      601 ccctattggc ttttacaggt ataagaaaag gagaagcttt tgctctgacc tggtcggata
      661 ttgactttaa gagcaaaaca ctcaatatca ataaaactgt aacaagaggt tatcaaggaa
      721 gattgatcgt aaatactcct aaatcaaaat ctggaaaaag aaaaatttat ctggacaatg
      781 atttgatcag tttattgaga aagtattata caaaaaacaa aacgatcgtt acaatccaaa
      841 gggaaaactt aatttttcac catgatggtc ttctctataa ccctacagtt tcacgttcgt
      901 ggcttaatgt catatataag catcatccag aattgactaa aagaatcact actcatggtt
      961 ttcgacatac tcatgcctct ctcctttttg aatcaggagc ttctttaaaa gatgttcagg
     1021 aacgtttagg gcatgcagac attcaaacta cttcaaatat ttacactcat gtaactgaaa
     1081 ctcaaaacaa aaaagtcatc aataattttg tagcatttat gaagaacagt actcaggggg
     1141 agtcaaaagg ggagtcaaaa aacattttag gctaaatata caacaaaaaa gacacgatcg
     1201 aaatcgtgtc taatcgttga
//
LOCUS       DBNICI010000269         1214 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.280, whole genome shotgun sequence.
ACCESSION   DBNICI010000269 DBNICI010000000
VERSION     DBNICI010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.280"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..797)
                     /locus_tag="AC1ZCC_002835"
     CDS             complement(<1..797)
                     /locus_tag="AC1ZCC_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIQ1631033.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFIL"
BASE COUNT          393 a          255 c          213 g          353 t
ORIGIN      
        1 cataagataa agcttggatt caataaataa ataaatgttt tggttaaagc tgtgaccagc
       61 aaatgaggaa ccgtgcgagc cagttgatct gccaatgcaa acatttcttt aggtgaaggc
      121 gctggcgtgt ctgccaacgg atactccagt cccaatgctt ggtaaccgtg cataccatag
      181 cagtggtacg gtagtaattc taacggtacg aaccgatcaa gatctgccac aaaacaagca
      241 atcgtcagca gctcggtcag gttcatattg atacctggaa ccacaggaac tcgaattcgc
      301 acttcgcagt taggatactc aattaaaaag cgacgaatat tcttcaaaat ttgttggtta
      361 tctaagccgg tccattgttt gtggagcttg ctggtaaaca tttttacatc cacaaacaat
      421 gtatccacat agggagctac cttttggatt tcattataag caccacaaaa gctggtttcc
      481 agtacggtat tgattccaat gtatttcgat ttttgcaaaa tttctttagc aaaatccgcc
      541 tgaaccagtg cctcgcctcc gctaatggtc actccgccat ccgagtgaaa gtaaaaaaca
      601 gcatccttct caatctcatc cattacctct tcagcggtca tggtctggcc aaatcctttt
      661 tcaatctgct tgctttgtga ttcgggaatc gaacaccagg cacagcgtaa tgggcagccc
      721 tttaaataaa caacggtccg taaaccagca ccatcatgta gagaaccctg ttctattctt
      781 aatactgttc cttccatata gatttttgaa ttacacacta gtttaaacta aaacacacaa
      841 ggaaaaactt accatttaat cataaaatta gctcatataa atatactagg atgtaaaata
      901 gactggtcaa aatctggtca aaaaagagga ctaatacagg tagtcctctt ttttgttatt
      961 ttaatattct aaatcatttt ttattggttt attcccttat tcgatgtaga ctcaaatcac
     1021 tatatcacta gatatcaaac ggcgtcatac ggaatcaaaa acatcaaatt tgacctgtta
     1081 aatggtcatt gaataacatt caaaatcaat aaaacggagt cagacacgga gtcaaaaacg
     1141 ctgagagtaa aaaatatata cagccttgta ggtaaagtac cacagggctg tatatttttt
     1201 atttcagatt acct
//
LOCUS       DBNICI010000270         1193 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.281, whole genome shotgun sequence.
ACCESSION   DBNICI010000270 DBNICI010000000
VERSION     DBNICI010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.281"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(102..626)
                     /locus_tag="AC1ZCC_002836"
     CDS             complement(102..626)
                     /locus_tag="AC1ZCC_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIQ1631034.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(619..1092)
                     /gene="tsaE"
                     /locus_tag="AC1ZCC_002837"
     CDS             complement(619..1092)
                     /gene="tsaE"
                     /locus_tag="AC1ZCC_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIQ1631035.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
BASE COUNT          312 a          301 c          173 g          407 t
ORIGIN      
        1 tcaaaaaaaa tggactgcga cacgactttt gtcacagtcc cttcgtgtgt ttttctgcct
       61 tctcaaaagg atgacagttt ctcactcttt tcctatttct tctattcaat caaataactc
      121 attttatata catctaaaaa ttcacctaga tctgttcttg cccctctagg catgaccgct
      181 tcgatttgga agcccatttt tcggtaaagg tgtacggctc tttcattacg aacttgaacg
      241 tcaagctcca atcgaaaaag tacacccatt tccttcgccc aactgatgat ttcttctagc
      301 ataagagtgc ctagtcccat tccccaatat tcctgcagaa tactgatacc gacttctccc
      361 acgtgagaca aacgaaattg actgtctgcc ttgacagatg ctgttccaat aatcgtccct
      421 tcatagattg caagcagcaa taaattatta ttgctttcgt atatataatc caactcttct
      481 tctaaggttt ctggtggtaa caggagttct gcttcatcca aaactaaaaa ttctgtctcc
      541 ttattaactt gtcccatcat caaaagaatg tcttttgcat ctcctggcac tgcttcccgc
      601 aagatgatcc caacttcttc actcatgatt ttcagcccat ttttctagaa ttcgctgttt
      661 gaaggtttca ctttgctccc cgtatgagat tagtttcaca taccgctttt ctaaatctgt
      721 atcacttttt tctaaaatca gttccaaata atcctccggc agtgcttctt ctaatagatt
      781 tccccattca acaacggaaa gcccatcacc ttcaaaatat tcatctaacc ctaaatctgc
      841 tccgctagct gctacccgat aaatatccat atgatacaac ggaatccgac cttgactata
      901 ttctctaata atcgtgtatg tcggactttt gatcatttgc gttatcccta atccttgggc
      961 aattcctttc gtcaacgttg ttttacccgc acctagatca ccagtcaata tcagattgtc
     1021 acctggcatt gcggaatgac caattgcctc tgcgaattgt tctgtcattg ttaatcctga
     1081 tagttcgatc atttttcatc ttctcccgct cttctttctt tttgccagta tatcgcgtaa
     1141 tgaagttttt ttaaagaaaa aatgaactag tcaagaacag cagctgctgt ttt
//
LOCUS       DBNICI010000271         1192 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.282, whole genome shotgun sequence.
ACCESSION   DBNICI010000271 DBNICI010000000
VERSION     DBNICI010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.282"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..1076
                     /locus_tag="AC1ZCC_002838"
     CDS             <1..1076
                     /locus_tag="AC1ZCC_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HIQ1631036.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          330 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tc
//
LOCUS       DBNICI010000272         1182 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.283, whole genome shotgun sequence.
ACCESSION   DBNICI010000272 DBNICI010000000
VERSION     DBNICI010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.283"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(31..1014)
                     /locus_tag="AC1ZCC_002839"
     CDS             complement(31..1014)
                     /locus_tag="AC1ZCC_002839"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HIQ1631037.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
BASE COUNT          323 a          263 c          227 g          369 t
ORIGIN      
        1 acctctaatt cacggtgttc aagcaagaaa tcattgttca ctgaaaagcg ttggatgttc
       61 tttttcaaag gtgtctaaga tctctcgtac ttcatcagta gatttcgtat tcatcaactg
      121 aactcgcaag tcatttgctc ctggaaatcc tttgatatag attttaaaga aacgatgtag
      181 tcctgtgatg gaacgcggga tgatttctga ataatgatct tgcagatcta gttgcagacg
      241 aagtagtccg atcagttctt tttctgtatg ttccctcggt tctttttcaa atgcataagg
      301 attcttgaaa atcccccggc caatcatgat gccatctacg ccatattttt cggccaattc
      361 ttctcctgtc tggcgatctg ggatatctcc gttgatcgtg atcagtgttt gcggagcgat
      421 ttggtcacgt aaagcaatga ttttagggat gacttcccaa tgtgcatcta ctttactcat
      481 ctctttacgc gtacgcaaat ggacagaaag attggctata tcttgatgaa gtaggtgact
      541 gatccattct tccatctcag acatttccgt atagccgata cgcgtcttga cgctgacagg
      601 caaaccgcca gctttagccg cttcaatcaa ttgtgccgct acttctgggc gaaggatcaa
      661 gccactgcct tttccacgtt ctgctacatt agggactggg cagcccatgt tgatatcgat
      721 tccttggaag cccatttctg ccatagcaat gctcatttct ctaaaaaatt ctggcttgtc
      781 tccccaaata tgggcaacca tcggctgttc atcttctgta aaggtcaatc ttcctcgtac
      841 actgtctttt ccatctggat gacagaaact gtctgagttt gtaaactcag taaagaagac
      901 atccggtgct gctgcatgct ttacgacatg gcgaaataca acatctgtta catcttccat
      961 tggtgctaaa acaaaaaaag gctttggcag ctctttccaa aaattagtag tcatttattt
     1021 gttactctcc attctatcaa gcagtgaagt cgcaatcttt ctcaaaaaca ttgtcattat
     1081 actaggaaac aaaaaaagaa gcaaagtgaa cagcttaaaa gatgtttctg attgagcaaa
     1141 aaaagactgt gacaaaaatc ttatcacaat tccttttccg ga
//
LOCUS       DBNICI010000273         1181 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.284, whole genome shotgun sequence.
ACCESSION   DBNICI010000273 DBNICI010000000
VERSION     DBNICI010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.284"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..186
                     /locus_tag="AC1ZCC_002840"
     CDS             <1..186
                     /locus_tag="AC1ZCC_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631038.1"
                     /translation="DQLEEAGIEGIKNDRSSWSKLRYYFALYIDTTQLRNLAYTKLLF
                     VDCVKGLQKHLNELEQV"
     gene            191..355
                     /locus_tag="AC1ZCC_002841"
     CDS             191..355
                     /locus_tag="AC1ZCC_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631039.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            348..650
                     /locus_tag="AC1ZCC_002842"
     CDS             348..650
                     /locus_tag="AC1ZCC_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631040.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            647..808
                     /locus_tag="AC1ZCC_002843"
     CDS             647..808
                     /locus_tag="AC1ZCC_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HIQ1631041.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            805..1110
                     /locus_tag="AC1ZCC_002844"
     CDS             805..1110
                     /locus_tag="AC1ZCC_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631042.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            1110..>1181
                     /locus_tag="AC1ZCC_002845"
     CDS             1110..>1181
                     /locus_tag="AC1ZCC_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HIQ1631043.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKK"
BASE COUNT          447 a          189 c          290 g          255 t
ORIGIN      
        1 gatcaactcg aagaagctgg aatcgaagga attaagaatg atcgttcgag ttggagtaag
       61 ctcagatact atttcgcact ttatatcgat acaacacaat taagaaattt agcatatacc
      121 aaattactat ttgttgattg tgttaaaggc ttgcaaaaac atcttaatga acttgagcag
      181 gtgtaatcag atggacctaa agacatttac agcacagatt gaactaatgc atcaagaagc
      241 tttaagacaa agtgtatcgt acgaagacaa gtggctcaac acgttccacg gcggacgtga
      301 gagcgcactt gatcaagtac tcaaattact gaaaggagaa tgtcgggatg gataagaaag
      361 cggcaatgca gcgaattatc gaattgactt attcagaaga ttggcaaaat gacaaagaag
      421 ctgcttcaga agtgatgagg cttggaagag cgatgtgggc agacaagagc aacaagccaa
      481 gaccacgaaa aatcgcaatt tggcacggtg acaaacttct agtgataggg acagctgaac
      541 agttagcaag tctcacaggc ttgcacgaga aaatcgtgag gaaaagagca aggtgtggct
      601 acacagacgc taagaagaga acgtttagat acgtggagga atcatcatga caacagaaga
      661 agtgattcaa atgcgtattc gaagccttca gcgtgagatt gacgatctgg aacgaacaaa
      721 ggcagtgatg gtcaatgaaa cggcgagaaa ggcaatcgat ttgcacatag agaatttaag
      781 aagggaaatc catcgattgg aggaatgagc gtggataaga aagcagcaat gaaaagaatt
      841 gctgaattaa ccaagtcaga atcttggcaa gaagacaaag aaatagttgc agaagtccaa
      901 aagctcggta aatcaatgtg gactgaaaag cccaaacgga gaacgccgag aaaaattgca
      961 atctggcatg atgaccgaat tttagtaaca ggtactgctg aacagttatc tgaaattact
     1021 ggattaagca aaaacattat ctgggataga gctaggagct tatggattga ttcaaaagga
     1081 cgacagttta ggtatgtgga ggagagataa tggatctcat tacacaatac agtgacatca
     1141 tcctcaagaa aatcatgatg aagattcaga aagataaaaa a
//
LOCUS       DBNICI010000274         1172 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.285, whole genome shotgun sequence.
ACCESSION   DBNICI010000274 DBNICI010000000
VERSION     DBNICI010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.285"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            238..885
                     /locus_tag="AC1ZCC_002846"
     CDS             238..885
                     /locus_tag="AC1ZCC_002846"
                     /inference="COORDINATES: protein motif:HMM:NF014505.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIQ1631044.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
BASE COUNT          447 a          130 c          213 g          382 t
ORIGIN      
        1 aggggaagta cataatgcaa atttgtccat aacttattga ttatcttgta aaaatgcggt
       61 aatttaatag ttagtgagaa gaggtgctta attatagtta aaaaagagga aatagaagca
      121 gcatgtaacc aagcaaatgt taggtttatt ggagaagctt tatctggata tcctaagtat
      181 aaatacttac acaactggtt acaaagtaaa agtttttatc ttaataaaga aagatgcatg
      241 cctaataaaa attatagggt ttataaaaga ggaactattg tatatgttga ttttggggtg
      301 aacgtgggtt atgagttatc tggtaatcat tttgctattg ttttaaataa taaagataat
      361 tcgaaaaatg gtgttgtatg tgtagtacca atctcttcaa aaaagaaaag taattatgta
      421 tctgtaggaa aaatattaga agttgctagt attaaacaat ttataagcca agctgataaa
      481 ttaaaagaga aacttaggat aattatttta ctttcattaa atagaggcat cattgatgaa
      541 agcgatttga atagttttat taaagatatt gaagctaaag gtgaaaagct ttcagtggaa
      601 gaagtagaaa aacgagctgt aaaacataat ttggtttgcg agacaaggga acaagtaatt
      661 aaagaaataa aatcattatc tgaagatatg gtaaaatatc agaaggtgtt taattgctac
      721 aagaaatata cacaagaatc ctatgtaatg ccacttaata tccaaacgat tagtaaaaat
      781 agaattagaa gaattaacaa gtttgatcct tctggaaata tgaaggcacc tggaaatgtc
      841 ctcaatgaag tagatgaaag tttaaaaaaa tattttacaa agtaaacgat atttttgttg
      901 acatttttct gttcgttagt taccatgtag ttatagaagg acgtaagcgc ctatttttaa
      961 tgtacacatc gggacgtatg cgcccacata gaaacatagt tgagagacat ctatatggtg
     1021 tctctttttt tattaatatg caaaaaagtc tagttttttc aataatattt atgtaataat
     1081 aaattagcca ccacaacaaa gaatgaaacc cattattatc tagtctatgt ccattctttt
     1141 tgtttgcagt agttgtgatg gctttccgta ct
//
LOCUS       DBNICI010000275         1160 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.286, whole genome shotgun sequence.
ACCESSION   DBNICI010000275 DBNICI010000000
VERSION     DBNICI010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.286"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..257
                     /locus_tag="AC1ZCC_002847"
     CDS             <1..257
                     /locus_tag="AC1ZCC_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HIQ1631045.1"
                     /translation="EMAETGAGVRSSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNW
                     SKKLHQDRFKFVEKYKEILEEYLRRTANDKKTRSIQHGFI"
     gene            217..462
                     /locus_tag="AC1ZCC_002848"
     CDS             217..462
                     /locus_tag="AC1ZCC_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631046.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            647..1114
                     /locus_tag="AC1ZCC_002849"
     CDS             647..1114
                     /locus_tag="AC1ZCC_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HIQ1631047.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
BASE COUNT          409 a          176 c          258 g          317 t
ORIGIN      
        1 tagaaatggc tgaaacagga gcaggagtgc gaagtagcag gcattggaaa gcagcagcaa
       61 acattgaatt ttattacaac gaaattcaaa aagggttcga tcagatgcgt gagctggatc
      121 ggcaaacaaa ttggagcaag aaacttcatc aagatcgttt caaatttgta gagaagtata
      181 aagaaatatt agaagagtat ttgaggagga cagcaaatga taaaaaaact cgttcaattc
      241 agcatggatt tatatgatat cgaatcagga gcaacactat ctgtggaatc ggaccatcta
      301 atcataaatt ttggtggaaa gcgccagatt attttgtggg tagttgatga tgtactgttt
      361 ccagaaattg ttcatgattt cgaagaatca aaagcggttg agtttgaaat agtgaaaaaa
      421 gtaatggaat tgattgaaaa atacgaggag gacagcgaat gataccgaag tttagagcgt
      481 gggatagaga aaaagacaga atgaaggcag tagctttggt tgaatttgct gaggcgtata
      541 aatcaggaga actcatttca cgtacaaaat aaaaaaagtc ggattcctcc gactatgaat
      601 aatatttccg acataagtat tatatcataa ttgggggaat cagaggatgg tacttttcga
      661 tgtaaagaaa tatgagacac caagcgcgaa agatgttgat atggaacgca caaaacataa
      721 tgtcgctgtg tttctttcag catatctatc agctagatgt agagtagggc aacctcgaga
      781 accaaaagtg acagcatctt attccttggt tccaccttct acagctgatc atatatttga
      841 agcagaaaga atgctgatcg ataaagaaga agcacaggaa gaatttgagt atctgcataa
      901 attgtttgtt cgaggctatt cagcgataca gcatccgcat aagcctgatg tgactgaaag
      961 gcgcaagaag atattctatg atcgttatat caatggtctg cccatttatg taactgctca
     1021 aaggaataat actagcgaag aatcggttaa agtagaatca aacagaatta tcatccaatt
     1081 tgcttcatcg ttagaactgg ttgctttcaa gtagccagtt tttacacttt ttataccctt
     1141 ttattaccaa tttggtttcc
//
LOCUS       DBNICI010000276         1155 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.287, whole genome shotgun sequence.
ACCESSION   DBNICI010000276 DBNICI010000000
VERSION     DBNICI010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.287"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..430)
                     /locus_tag="AC1ZCC_002850"
     CDS             complement(<1..430)
                     /locus_tag="AC1ZCC_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HIQ1631048.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
     gene            774..>1155
                     /locus_tag="AC1ZCC_002851"
     CDS             774..>1155
                     /locus_tag="AC1ZCC_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6941 family protein"
                     /protein_id="HIQ1631049.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIK"
BASE COUNT          388 a          174 c          180 g          413 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tctaactttt ggggtgcaca
      481 tcatttttga ggggaagtac atatagttgt tgatatagtt gctaatattg ttagctaatt
      541 tgttagtaaa actgtatacg aatttagata gtaaaaaaac agtttgttcc ttatatagag
      601 gcacttttac tactgattaa atgtcagata tgcttacaaa tatacttata aatatgctta
      661 caaaattagc taatatttcc gttgacaaag ctttgttact attgttaaac taaattgact
      721 agcaaaagtg aaacataaat ttattaaata aatgtaaaaa ttaggagaaa tcaatgtctt
      781 atagagtaca attaataatt tcagaagatg tagaaagtca gcaatttggc actaatgtta
      841 ttaataaagt aattaatcca gctctttcta ttaatgcgcc tttaattcct actgctttat
      901 cattttctgt aacagctgtg gtgtctgaga tagaggatac agaaaagata aaaatagtag
      961 aaattgaagt tttaaacaag aatgaaaaac aaattttttc tacgggtgaa gtatcggtaa
     1021 gcttgcctcc tcaagttaac gatataaact ttaatataaa cgccagaaat gtcttggttg
     1081 aagaagcagg agagcattat gctgttgtta aatttaatgg aactgagatt ggaaggcaaa
     1141 tctttgatat caagg
//
LOCUS       DBNICI010000277         1147 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.288, whole genome shotgun sequence.
ACCESSION   DBNICI010000277 DBNICI010000000
VERSION     DBNICI010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.288"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..921
                     /locus_tag="AC1ZCC_002852"
     CDS             <1..921
                     /locus_tag="AC1ZCC_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HIQ1631050.1"
                     /translation="DDPYWLGKAPLGENIANKSVTFPIKSSAYSLYQANMEEEFVLGQ
                     TYTITMKATKPPIQTFIVYNEDSGDYRYGNLEPVEGLVDTWGLTFTPQKVGVNYPKRL
                     TIIQYPQSTTGACQIDWLKIEKGDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYT
                     EKGLNDAVNVYDPQRVEGLKNFADGIQIAGDKVISENDCTVYTLTKDNSQSFIDGYAT
                     FIKHGKEVIVNGTVKFKKAYAFGVPLDDEVPDEFVAKIVHGMLTGPSGTSSVSKAMYV
                     RKDLGTIITNSEFAANEWFTFHGNYWVGEE"
     gene            924..>1147
                     /locus_tag="AC1ZCC_002853"
     CDS             924..>1147
                     /locus_tag="AC1ZCC_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631051.1"
                     /translation="MKNIWKYGRTGGEYAGQVLDDMVMTVPFTDVPPLEGIRADGEPL
                     TIADQMFDPKLNQWIVLVNVLDHNDLNNLKA"
BASE COUNT          389 a          193 c          251 g          314 t
ORIGIN      
        1 gatgaccctt actggctagg taaagcgcct ttgggtgaga atattgctaa taagtctgtt
       61 acgtttccaa tcaaatctag cgcctacagc ctataccaag ctaacatgga agaagaattt
      121 gttttaggtc aaacctatac aattaccatg aaggcaacca aaccacctat ccaaacgttc
      181 attgtttaca acgaggatag tggagattat agatatggta acttagagcc agtagaaggg
      241 ttagttgata catggggtct gacttttaca ccgcagaagg ttggagttaa ctaccccaaa
      301 cggttaacta ttatccagta tccccaatca acaacaggtg catgtcaaat tgactggctc
      361 aagatcgaaa aaggcgacac acgaaccccg aatattagtc agtttaaata ctttggtgaa
      421 ggcttgaaag acagtaacaa tcccaatgat tacagttggg atgtcacacc tgaatatact
      481 gaaaaaggct tgaatgatgc tgttaatgtg tatgatcctc agagagttga aggtttgaaa
      541 aactttgccg atggtattca aatagcagga gataaagtga tcagtgaaaa tgattgcact
      601 gtttatacat taactaaaga caacagtcaa tcgtttatcg atgggtatgc aacatttatt
      661 aaacacggaa aagaagttat tgtaaatggt acagtaaagt tcaaaaaagc ttatgctttt
      721 ggtgtaccac ttgatgatga agtcccagat gaatttgtcg caaaaattgt tcatggcatg
      781 cttacaggtc cgtcaggaac aagtagtgta tcaaaagcga tgtacgtgcg aaaagactta
      841 ggaacaatta ttacgaatag cgaatttgct gcaaatgaat ggtttacatt ccatggtaat
      901 tattgggtag gggaggaata aaaatgaaaa atatttggaa atacggacga acaggtggag
      961 agtatgctgg acaagtgctt gatgatatgg ttatgactgt tccatttacc gatgttccac
     1021 cactcgaagg aattcgtgct gatggggaac cactaacgat tgctgatcag atgtttgatc
     1081 ctaaactgaa ccaatggatt gttttagtga atgtactaga tcacaacgat ttaaacaatc
     1141 tcaaagc
//
LOCUS       DBNICI010000278         1132 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.289, whole genome shotgun sequence.
ACCESSION   DBNICI010000278 DBNICI010000000
VERSION     DBNICI010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.289"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            139..459
                     /locus_tag="AC1ZCC_002854"
     CDS             139..459
                     /locus_tag="AC1ZCC_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631052.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="AC1ZCC_002855"
     CDS             912..1112
                     /locus_tag="AC1ZCC_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIQ1631053.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
BASE COUNT          387 a          190 c          210 g          345 t
ORIGIN      
        1 aaaagaggtt gtgacgtggt cacgacctct ttttgacgat tatgatacaa aaaacatcat
       61 ttatgaacaa aatgtgaata acgggcgttc ttgagatatg attttaattg ggaaaaactt
      121 atcgataagg aggtctttat gaaaaaaaga ctgttatggt gtttgctgtc tctatcccta
      181 agtttaacaa gtcagccagt ttttgctgaa gaaacacagc ggtttcctat tacaggcaca
      241 cgtctgtaca aaaatgttta tgtacagaca ccagatcttg attctgcgat tcctgaaaca
      301 gaagaacggt ctttgcagcc aagatttggt tacttttggt actgtccgac ctgtgattat
      361 gaaagttgct ggcatttctt acattctaca gctgcagctt ttgcttttac tcatactcag
      421 aaatatgatg gacatcatcc tgttatttat gaggtataga tatgagcaaa aaagaaaaac
      481 taatctcgtt ggtcgtattg gtttgccttg ttcttctggt aactgtcata ggctatcaga
      541 aataccatga gaacatcaga cctactaaag aatcttcccc agcctcgacc gttgaaacag
      601 aaccgataag tagtcagaaa gaataatttt tttagtttaa aaaagaacat cagagcaaaa
      661 aaacaccaac tgaacaatat gtcgtttatt ttaaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa ta
//
LOCUS       DBNICI010000279         1119 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.290, whole genome shotgun sequence.
ACCESSION   DBNICI010000279 DBNICI010000000
VERSION     DBNICI010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.290"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..1022
                     /locus_tag="AC1ZCC_002856"
     CDS             <1..1022
                     /locus_tag="AC1ZCC_002856"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HIQ1631054.1"
                     /translation="LRAIEDQEIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNG
                     IEGYVPETGYNQTFHGKAAPAYNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAK
                     ATIPSPTMILRQEILSNDGSSKIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQF
                     DDTNWAFLADQTKREALLQKGIDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHA
                     SSWLFSGGYEPIAEELFSTNYDGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKF
                     PDLEDTEKIKERIKEAQQFVPLENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVA
                     KEVWL"
BASE COUNT          393 a          186 c          219 g          321 t
ORIGIN      
        1 aacttcgtgc aatcgaagac caggaaataa aaaaattagt ggaagaccaa aaagcagtcg
       61 gcatcaaagg aattacagat ggtgaatttc ggcgtggatt ttggcatctt gattttttag
      121 aagaactaaa tggtatcgaa ggctatgttc cagaaactgg atataatcaa acattccacg
      181 gaaaagcagc accagcttat aatattcggg tagtagataa attatctttc aatccaaatc
      241 acccattttt aaaagatttt gcttttctga aagaagtagt ggaaacagat gatacttttg
      301 ttgcaaaagc aacgatccct agtcccacga tgattttacg acaagagatc ctatctaatg
      361 atggttcttc aaaaatcaag gagatttatc ctgatctatc tgatttttat gaggacttgt
      421 cctctactta caaagcagcg attcatgctt tttatgaaaa aggttgccgt tacctgcaat
      481 tcgatgatac aaattgggcg tttctagctg atcaaactaa aagagaagca cttcttcaaa
      541 aaggaattga tccgaaagaa atggcccata tttgtacaga gattatcaat aacgcactgg
      601 aagacaaacc agaagatttg acgatcacaa cacatatctg ccgcgggaat cacgcttctt
      661 cttggctgtt ttccggtgga tatgaaccca ttgcagaaga gttgttttca acaaattatg
      721 atggtttctt tttggaatac gattctgacc gagcaggaga ttttactcct ttaagacatt
      781 ggagtaacaa agaaagtaaa gttgttttag gattagttac ttctaagttt ccagatttgg
      841 aagatacgga gaaaataaaa gaacgaataa aagaagcaca acaatttgta ccattggaaa
      901 atctaagtat cagtcctcaa tgtgggtttg cttcaacaga agaaggaaac catttaacag
      961 aagaagaaca atggaagaaa atccaattat tgcagcaagt cgcaaaagaa gtttggctat
     1021 aaaaatgtga ggcttggcaa aaaaaagcgt cccaaaaagt cggcttttca tggagtggag
     1081 aaatccactc ttttttattt accttttata ttatttatt
//
LOCUS       DBNICI010000280         1112 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.291, whole genome shotgun sequence.
ACCESSION   DBNICI010000280 DBNICI010000000
VERSION     DBNICI010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.291"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(267..971)
                     /locus_tag="AC1ZCC_002857"
     CDS             complement(267..971)
                     /locus_tag="AC1ZCC_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631055.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT          321 a          194 c          199 g          398 t
ORIGIN      
        1 cttttttctc tatcagaaaa tatgtttatt attagaattt tttatagtta aggttaggca
       61 tcttaagaaa tcaggcaact ccaagatatc ttcaggtaga agagaagtcg cctaacattg
      121 taggatcata ggtgatttgt tggacaggtc aagctgatat ttgagcctga tgttagaagg
      181 aatttctttt tacttgctgt agaaattatt atatgactaa aaaggctggg attttaaatc
      241 ccagcctttt atgacaaata gaatttttat cgatcagtaa tcgctgtcgc tgcaaattgt
      301 tcgatcaaag gaatcaaagt caatttgact ttgtatttcc gttcgagtgc tttttcgata
      361 acgaactcat tttcagtagt catagaaata gttgctaatt tacgaggttc atcagcattt
      421 ttttcattaa taactacttc atgttcattt tgttctgtgt aaaatttagg atactcagca
      481 acggcttttg aaaaatcagc aaaacttaaa gcggcattcg tatcagttga gcgtgtattt
      541 tcagttgtgt ccgcttttgg tttttccgtt ttttcttctg gcacactgac tgtttcttct
      601 gtaactggtg tcgcaacagc ttgcgcagtt ggttcatcgt ttttcaatcg ttgatcattc
      661 cagtaagaca cgatttcaat aagaagattg tcacggaatc gttcgaaaat cccgttttct
      721 cctgaacgaa ttctctttaa tacagtattg aagttgtcat cgattggcac gttgatccgt
      781 gtatcttcat ttgttttccc agctaatccg atcgttaaga tttgacggtt tttgggatca
      841 agcatgactt gtaacggcaa accataacgt cgcaagctct cttcttctag agaacggaat
      901 aattgtcctc ttacaccatc caatgaagga cggaattgat ttaataattt ttgtttctgt
      961 tttttcttca tatgttctct ccttcgctca taccctttcg attataagaa aaaaacagcc
     1021 aaatgaaaag gagtaatgca aaaaaacttg cgtatacgca aattacaaca gaaaaatttg
     1081 cgtattttcc cgttttttga aagaaaaata aa
//
LOCUS       DBNICI010000281         1103 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.292, whole genome shotgun sequence.
ACCESSION   DBNICI010000281 DBNICI010000000
VERSION     DBNICI010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.292"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            2..1084
                     /locus_tag="AC1ZCC_002858"
     CDS             2..1084
                     /locus_tag="AC1ZCC_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIQ1631056.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          401 a          192 c          213 g          297 t
ORIGIN      
        1 tatcaatgat gatggctcaa gaaaaaaagt ggagaaagtt ggcggagaca cccgaccaga
       61 ggccgaagct gctttacgaa aagttttatc agatattgac gaaacaggac aatacttttt
      121 aggtacggat actcgagtaa aacaatacct tgatttttgg atggaggaat acgttaaact
      181 aaatctaaaa tacaatacct atgaaaacta ccgatttacc atcaaaaatc atataaacgg
      241 ttatttagga aagaaaaaac ttacggatct ctcccctgct cttttacaaa atttcatcaa
      301 tgctgaattt aaaaagggtt actcgaagaa aacaatgact attactcact ctgtccttaa
      361 gaatgcgctg aatatggcgg tttatccttg gggattaatc aagcaaaatc ctatgctgta
      421 tgtaaaaata ccaaaatatg aagcacgacc aacgactaaa aaagatctaa aaatcatttc
      481 tcttgaggac tttgatcata tgctagaaat tactcctgaa ggacatcctt tctatattcc
      541 tttgaatatt ggattttata cgggaatgcg cgttggcgaa gtttgtggtc tgacgtggga
      601 tgatgtcgat ttttcaaatg gaacaattac tgtagagaaa caaatggtaa agaatgacgg
      661 cgcatgggta tatggtacgc caaagacaag cagttccaat cgaacgattt ttattggaca
      721 aaccttgcta gcaattctga aaaaacataa gaaacaacaa ttagaaaatc gaatgaagta
      781 tggaaagctc tacattgatt caaatgcagt atgtacgaag gaagacggtg agctagttac
      841 gccaagtgta gtgaaatgga atactaggag gatatcgaat gcactctccc tctcttttaa
      901 cttccattct ctcagacata ctcatgctac acttcttctc gaaaatggcg caaaaatgaa
      961 agaaatctct gaacgattgg gccacagcag aatttcaatt acgatggata cttactcgca
     1021 tgtaacagat aagatgagaa atgaaacggt cgatatcatg gaaaatctta gaaagaattc
     1081 ttgatttttg ccaccgaaaa aaa
//
LOCUS       DBNICI010000282         1085 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.293, whole genome shotgun sequence.
ACCESSION   DBNICI010000282 DBNICI010000000
VERSION     DBNICI010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.293"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            2..>1085
                     /locus_tag="AC1ZCC_002859"
     rRNA            2..>1085
                     /locus_tag="AC1ZCC_002859"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          332 a          215 c          318 g          220 t
ORIGIN      
        1 ataagggcgc acggtggatg ccttggcact aggagccgat gaaggacggg actaacaccg
       61 atatgctttg gggagctgta cgtaagctat gatccagaga tttccgaatg ggggaaccca
      121 acatctttaa taggatgtta cgattgtgtg aatacatagc acattcgagg tagacgcaga
      181 gaactgaaac atctaagtac ctgcaggaag agaaagaaaa ttcgattccc tgagtagcgg
      241 cgagcgaaac gggaaaagcc caaaccagca agcttgcttg ttggggttgt aggactccaa
      301 tatggtagtt ctttcagata gtcgaatgac ttggaaaagt cagtcaaaga gggtaaaaac
      361 cccgtagacg aaatgtggaa gacacctagg aggatcctga gtacggcgga acacgagaaa
      421 ttccgtcgga atccgggagg accatctccc aaggctaaat actccctagt gaccgatagt
      481 gaaccagtac cgtgagggaa aggtgaaaag caccccggaa ggggagtgaa atagaacctg
      541 aaaccgtgtg cctacaacaa gtcaaagccc gttaatgggt gatggcgtgc cttttgtaga
      601 atgaaccggc gagttacgat tgcatgcgag gttaagttga agagacggag ccgcagcgaa
      661 agcgagtctg aatagggcgt ttgagtatgt agtcgtagac ccgaaaccat gtgatctacc
      721 catgtccagg ttgaaggtgc ggtaaaacgc actggaggac cgaacccacg tacgttgaaa
      781 agtgcgggga tgaggtgtgg gtagcggaga aattccaaac gaacttggag atagctggtt
      841 ctctccgaaa tagctttagg gctagcctcg gaattgagaa tgatggaggt agagcactgt
      901 ttggactagg ggcccatctc gggttaccga attcagataa actccgaatg ccattcattc
      961 atatccggga gtcagactgt gagtgataag atccatagtc gaaagggaaa cagcccagac
     1021 caccagctaa ggtcccaaaa tatatgttaa gtggaaaagg atgtggggtt gcacagacaa
     1081 ctagg
//
LOCUS       DBNICI010000283         1083 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.294, whole genome shotgun sequence.
ACCESSION   DBNICI010000283 DBNICI010000000
VERSION     DBNICI010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.294"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(277..732)
                     /gene="tnpA"
                     /locus_tag="AC1ZCC_002860"
     CDS             complement(277..732)
                     /gene="tnpA"
                     /locus_tag="AC1ZCC_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HIQ1631057.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
BASE COUNT          305 a          213 c          184 g          381 t
ORIGIN      
        1 attatttctg cgccagcatt ttctaatgtt tgaatttgtg ttttgtaatc ttggttgctt
       61 gtgctaattc ttgaatagcc gtaaattgcc aaaatttcat caccctttct ggacataagt
      121 ttctgacacc agttaaaatc aagtctatca gtaacccata cacccgtgag gacgggtgta
      181 tttttaatcc ccctagaagg ggtcgacact cgctgcccct taaaagggga tttttaaaag
      241 accgccaact gctcatcttc actcacttac ttctctttat tttttaaatg gttctacgta
      301 ctctctttta cttataatat atcgaatgcg atcatcagct tcttgttcac ggatgtattt
      361 ttcatttgtc ttttgattca atccgactgt actcacatag tatctcttcg accaaaagga
      421 tttgtttccg tatttatatt tgagatttgc gtgctgctcg tggattagta ccgcactacg
      481 acctttcaga taccccataa aactggatat acatattttt ggtggtactc gaacaagcat
      541 gtgaatatga tctagcattg catgtgcttc tatgatttcc acatctttca tttcgcacaa
      601 cttcctaagt atttttccaa gatcttttcg taattgccca tagattactt ttcgttgata
      661 ttttggtgta aaaataatat gatacttaca attccaacga gtatatgcta aacttttatc
      721 gtcatttgac atgaaaaaac tcctttcgat ctgatgagcg gttggtagcc actcactagt
      781 atatcgaaag gagtttttat cgctaaagat acttcttcca cgcgtagaaa cgcgcggttt
      841 tcttgtttcg cacgctttac gcacaaaact gactgaagtc attaagaaaa taaaagggtc
      901 taaaactttt aagttttgta ccgaccccca aaagttagac caaaaaatct aacttttgga
      961 ggtcggtatt ttttatggtt aaatatactt ttgaacttaa attaaagatc gttcatgatt
     1021 atttggatgg caaaggcgat tggaccgtaa aaatccagat caagagattg aagcggaaat
     1081 gct
//
LOCUS       DBNICI010000284         1058 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.296, whole genome shotgun sequence.
ACCESSION   DBNICI010000284 DBNICI010000000
VERSION     DBNICI010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.296"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(36..992)
                     /locus_tag="AC1ZCC_002861"
     CDS             complement(36..992)
                     /locus_tag="AC1ZCC_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631058.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgt
//
LOCUS       DBNICI010000285         1050 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.297, whole genome shotgun sequence.
ACCESSION   DBNICI010000285 DBNICI010000000
VERSION     DBNICI010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.297"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            89..1024
                     /locus_tag="AC1ZCC_002862"
     CDS             89..1024
                     /locus_tag="AC1ZCC_002862"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIQ1631059.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT          366 a          166 c          238 g          280 t
ORIGIN      
        1 gttataaaaa tgaacattgc atagtagaga ttctttgatt atactaattc aaggttatat
       61 gaaaaaagag aaatgagatg gatgatttat gaaactttgg atgaaaatcc ttttaggcgt
      121 cgctttagct ctgattgttg cgttagtgtt tgcagggaat tatttttata attatgctgt
      181 tgtgccttct gagaaagact ttttagacga agatacacct ggtacaacac agacatccaa
      241 agaaacggat gcacataaat ggtttagttc aaaagaaaac cgaagcgatt ggaccatcac
      301 ttcaaaagac ggtctgaaat tatcagcgat ttatcttcct gctgaaacga aaagtgaaaa
      361 aacagctctt gtggctcatg gctatatggg aaacgctgaa acgatgacca attacgcaaa
      421 gatgtttcat gatatgggtt acaatgtcct tgttcctgat gcaagaggac atggaaaaag
      481 cgaaggggat tacattggat ttggatggcc agagcgtaaa gactatgtac agtggataaa
      541 caaagtgcta gaaaacaatg gaaaatcgca agaaatcgtt ctatacggtg tcagcatggg
      601 agcagcaaca gttatgatga ctagcggaga aaaactacca gataatgtga aagcaatcat
      661 tgaagattgc ggttattctt ccgttcatga tgagcttgct tatcagctag acgatatgtt
      721 tagtcttcca gcatttccgc ttatgcaagt aacgagtcta gtgacaaaag tccgtgctgg
      781 ctactttttc ggtgaagcaa atgcagtgga acaattgaag aaaaaccagc gcccaatgct
      841 ctttatacat ggagacgaag atacatttgt accatttgaa atgttagata aggtctatcg
      901 tgcaacgaag ggaccaaaag aaaagtatgt tgtaaaagga gccgagcatg cggaagcata
      961 caagacagat cctgaaaaat atcagcaagt agtccaacaa tttttaagcc aatatgttca
     1021 ttgaacgagt ttaaaagtag gggatgaaaa
//
LOCUS       DBNICI010000286         1013 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.298, whole genome shotgun sequence.
ACCESSION   DBNICI010000286 DBNICI010000000
VERSION     DBNICI010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.298"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..823
                     /locus_tag="AC1ZCC_002863"
                     /note="aph(2'')-Ia"
     CDS             <1..823
                     /locus_tag="AC1ZCC_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HIQ1631060.1"
                     /translation="IIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTN
                     LETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQ
                     MHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEG
                     KKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGED
                     ILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          432 a           78 c          160 g          343 t
ORIGIN      
        1 aataatcggt agtggttatg atagtgtggc atatttagtt aataatgaat acatttttaa
       61 aacaaaattt agtactaata agaaaaaagg ttatgcaaaa gaaaaagcaa tatataattt
      121 tttaaataca aatttagaaa ctaatgtaaa aattcctaat attgaatatt cgtatattag
      181 tgatgaatta tctatactag gttataaaga aattaaagga acttttttaa caccagaaat
      241 ttattctact atgtcagaag aagaacaaaa tttgttaaaa cgagatattg ccagtttttt
      301 aagacaaatg cacggtttag attatacaga tattagtgaa tgtactattg ataataaaca
      361 aaatgtatta gaagagtata tattgttgcg tgaaactatt tataatgatt taactgatat
      421 agaaaaagat tatatagaaa gttttatgga aagactaaat gcaacaacag tttttgaggg
      481 taaaaagtgt ttatgccata atgattttag ttgtaatcat ctattgttag atggcaataa
      541 tagattaact ggaataattg attttggaga ttctggaatt atagatgaat attgtgattt
      601 tatatactta cttgaagata gtgaagaaga aataggaaca aattttggag aagatatatt
      661 aagaatgtat ggaaatatag atattgagaa agcaaaagaa tatcaagata tagttgaaga
      721 atattatcct attgaaacta ttgtttatgg aattaaaaat attaaacagg aatttatcga
      781 aaatggtaga aaagaaattt ataaaaggac ttataaagat tgattatata atatatgaaa
      841 agctattata aaagacatta gtattaaata gtttaaaaaa atgaaaaata ataaaggaag
      901 tgagtcaatt ccagactcct gtgtaaaatg ctatacaatg tttttaccat ttctacttat
      961 caaaattgat gtattttctt gaagaataaa tccattcatc atgtaggtcc ata
//
LOCUS       DBNICI010000287         1006 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.299, whole genome shotgun sequence.
ACCESSION   DBNICI010000287 DBNICI010000000
VERSION     DBNICI010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.299"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..796
                     /locus_tag="AC1ZCC_002864"
     CDS             <1..796
                     /locus_tag="AC1ZCC_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HIQ1631061.1"
                     /translation="KLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNGGIKDEAL
                     TQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTKAAKMYKE
                     FGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFAKSVESKG
                     FEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYKKTKAAEA
                     EAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            935..>1006
                     /locus_tag="AC1ZCC_002865"
     CDS             935..>1006
                     /locus_tag="AC1ZCC_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HIQ1631062.1"
                     /translation="MDLITQYSDIILKKIMAKIQKDKK"
BASE COUNT          376 a          157 c          225 g          248 t
ORIGIN      
        1 caaattgggt gtagcaggag cagtaatcgt aggtattgga gttatcggag gatttaagtt
       61 cttcgaaaaa atcgataatg gatatgtggg tgtgcgctat tcaatgaacg gcggtatcaa
      121 agatgaagca ctgacgcaag gtgtgaaatt tgtagggatt gacaaagtga tccaatatcc
      181 aattcgcttg caaactatcc aatcaaaaaa tatttcagta tctacaagcg acggcaaaaa
      241 gacaacgatt gatatcaaat atgactacaa agttgattca actaaagcag caaaaatgta
      301 caaggaattt gggaatatca cttcggaaga tatcgaaagt ggatggttaa aatctaagct
      361 tcaaaaggtc gctcgtgaag tttatgcgaa atatagtctg cttgatgtcc tttcaggaga
      421 ttcctctaaa gttgaagctg aggtattaac gaactttgct aaatcagttg aatctaaagg
      481 gtttgaagtc gaagacgtaa cacttggtgt tccagatgtc gataaagaaa cacaaaaatc
      541 aatcgatgcg atcattcgag ctggtcaaga aaatgaaaaa gcgaagctag atgcagaaac
      601 tgcaaaaact caagctgata gtgaagctta caagaaaaca aaagctgcag aagcagaggc
      661 agaatctaat cgcaaagtcg ccgaatcagt aacagacaat ttgattcgtt atgaagaagc
      721 tcaagctcgc aaaaagcatg gatgggtaac agtaaatgga gcagatactg tagttacgga
      781 tgaagcaggc aaataatatg ggattcttta tggctaaaat tctcttgttc ttaggtttag
      841 taggagcagc atatctcgtg tatgcgctcc tttcccaaac tgatgacaaa gaagatgaca
      901 acaacgatga aatgaaattt taggaggaga aataatggat ctcattacac aatacagtga
      961 tatcatcctc aagaaaatca tggcaaagat tcagaaagat aaaaaa
//
LOCUS       DBNICI010000288         1004 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.300, whole genome shotgun sequence.
ACCESSION   DBNICI010000288 DBNICI010000000
VERSION     DBNICI010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.300"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..>1004
                     /locus_tag="AC1ZCC_002866"
     CDS             <1..>1004
                     /locus_tag="AC1ZCC_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HIQ1631063.1"
                     /translation="GKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLVTP
                     FCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPRCL
                     LFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHYIV
                     TDMNAPYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQYR
                     QLKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQDL
                     LTAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLENLH
                     "
BASE COUNT          301 a          209 c          191 g          303 t
ORIGIN      
        1 tggcaagaag atctctatga ttctcctgaa tcgtagcggt aataaacgga cctatcttcg
       61 gttgaagaaa cagcgatacc tctgccgagc atgtaaaaaa tattttaccg ctcggacgta
      121 tctcgttacc ccattctgct ttatttctaa gcagattcat tacaagatct tagaagaatt
      181 gacagagcgt caatctataa aagcaattgg taaacattgc gatgtttctg tgacaactgt
      241 tcagcggaca ttgtccgcct tgtcatcaga tatccgtcag aatcttgagt atttacctcg
      301 gtgtctatta ttcgatgagt ttcgttctct ttctacatgg cacggtaaat tttctttttc
      361 ttgtatggac ggtgagaccg ggaaactgtt tgagatctta cctagccgcc gtaaaaaaga
      421 tctcgttgct tttttcatgc gtttttctct caaagcgagg ttgggtgtac attacatcgt
      481 aacggatatg aacgctcctt atttctcact agttaaagag tgttttccaa atgcacaagt
      541 gatcgtcgat cggtttcaca ttgttcaaca tctgaaccgc ttcttcgatg cggttcgaaa
      601 acgtgtgatg aaaacacttg atcaaaaaga cccaatacaa gctaaacagt atcgccaatt
      661 gaaatcctta ttcaaattac ttttaaaatc agaggatcgt ttagattaca atacactaac
      721 gaaaagacgc aatttcaatt ggagactatt gactgaaaca gaagtagttg accgccttct
      781 aaagatttct cctgaactta aaacagctta tcgttactac caagacctgc ttactgcgta
      841 tcaggaaaaa gatccagata cttttttcca gctgcttcga gcaatgcctg gagaagttcc
      901 attggaactg caccatatca aaaaagcctt tttcaaatac gagaaaggga ttcggctggc
      961 attactgaag aagtattcga atgcacgact ggaaaatctc cata
//
LOCUS       DBNICI010000289         1003 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.301, whole genome shotgun sequence.
ACCESSION   DBNICI010000289 DBNICI010000000
VERSION     DBNICI010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.301"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(37..900)
                     /locus_tag="AC1ZCC_002867"
     CDS             complement(37..900)
                     /locus_tag="AC1ZCC_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIQ1631064.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
BASE COUNT          267 a          241 c          156 g          339 t
ORIGIN      
        1 aatttctggg cttttttaca gaatgagtta ctgcttttat cgactgattc ccatcgaaaa
       61 aatcgcgata cagccatagc ctgtgtgact ggcgatcgtt ggtcctaagg gataaagctt
      121 cacatctttc atctttatct gctcttccaa aagccgtttt gccttctccg cgctctctag
      181 atccccagcg taagccacat aaacgatttg atcaagtggt gcaacgattg tttttaccgt
      241 ctcattgacg attgtctgga tcgacttgtt tcttcctcga actttgtcaa cattcgtcag
      301 ccttccttct ttatccaccg taataatagg ctttacactc aatagactgc caagagtagc
      361 acttgctttg gaaatcctac cgcctcgttc tagatatttc agatcgtcta ctgttaccca
      421 tgaatggact ttcaaataat tactttccag ccactctagg gtttcttcga tgcttttacc
      481 ttcttttttc aatcttacag cttccaatac taaaagccct tccccaatac ttgcagcttt
      541 tgtatcaaaa acatagatct cagggtcttc atattcttcc ttcagcaatt caaccgcttg
      601 aagcgcacta ctgtaagagc cgcttaatcc actggaaaaa gccacatata aaacaggaat
      661 ctgtttttcc gcataagaac ggaaaaattc taaatatcgg ccaacattga tttgtgatgt
      721 cgtaggcatc gcaccatttt tcagctgatt catgtagcgg tcataattga atgtctttcc
      781 gaggtcatct actaactctt gatcattcaa ttggagattc attgataaaa aatcaacttt
      841 ttcttttgct aatacatggt atggcagatc acaacaagaa tctgttacaa tttgatacac
      901 atttttcaac cccttctcgt tacattttac ccttatcgtc tattatagca aatctcttgc
      961 gctagacaca ctttaaccgg tttattttcg caagttctaa tca
//
LOCUS       DBNICI010000290          998 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.302, whole genome shotgun sequence.
ACCESSION   DBNICI010000290 DBNICI010000000
VERSION     DBNICI010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.302"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(80..634)
                     /locus_tag="AC1ZCC_002868"
     CDS             complement(80..634)
                     /locus_tag="AC1ZCC_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIQ1631065.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADVAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          323 a          185 c          107 g          383 t
ORIGIN      
        1 ttccgaaaat tcagttatct tcattaatct caccttctaa gttttcatta tacacaaaaa
       61 atatatccta atatctcaat tagcctccca atttaaaacc acgtaacgat gcccctattt
      121 catcttcagt tataccaatg tacctttttg taattgcttg cgagctatga ttaaaaataa
      181 acattaaggt agcaatgtct ctagttttct tgtagtagtg atacccaaaa gtttttctta
      241 atgtatgtgt tcctatatca tctcttccta atttttctgc tacatctgca tatatctgat
      301 agacacgatc ccttgttata tgtcgacctt gttgccgact tggaaataac caatcatttt
      361 cttctttacc ttcagtatat aataaaatct cttctctcaa attatctata aaaatcggtt
      421 ttcgcttacc tgttttatgt tcaacaataa taggcttact tttacctcgt atgtctccaa
      481 cttttaatcc tataatatct gacatacgta acccattatt gattccaaac aaaaataaaa
      541 aactatctct ttctggatta cttgttcttc ttagccaaaa ctgaaaatct tgtatctctt
      601 catttgtcct taacggttgt acattatagc tcactattgt ttcccttctt aaaaatattc
      661 tgagttcatg attatatata tattttacca taatcatgaa cttatatata tactctgtta
      721 aaagtgaaga aaatcaaggg gtttagttta tgattttata tcatttcagt aactcagaat
      781 attccgactt ttttgtacaa aaaaattaac cttacttata tgaagattct aagctcttat
      841 ttaaatttta gacgggcagt tatccgtaga agttcttaga atagctaatt tacataatta
      901 aattatatga aatttaagtt tgaacaatat actaagaact ccccttacta taaagtgaaa
      961 taaattagca tatgaataac cgtgctacca aacttttc
//
LOCUS       DBNICI010000291          996 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.303, whole genome shotgun sequence.
ACCESSION   DBNICI010000291 DBNICI010000000
VERSION     DBNICI010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.303"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            10..735
                     /locus_tag="AC1ZCC_002869"
     CDS             10..735
                     /locus_tag="AC1ZCC_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HIQ1631066.1"
                     /translation="MKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAWAEVSKVC
                     EAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKAMKDEPYT
                     YEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLGLYIFAGE
                     DMPEDVSMLEPATQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITADDSKWTKR
                     DLGILKRGVNWIEDQYREETKEK"
     gene            741..>996
                     /locus_tag="AC1ZCC_002870"
     CDS             741..>996
                     /locus_tag="AC1ZCC_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631067.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RIIEVRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKE"
BASE COUNT          355 a          157 c          227 g          257 t
ORIGIN      
        1 gaacaacctt tgaaaaatag aagtgataat acgcttttta ataccttata caaaataaat
       61 gtgaaagatg ttactgaaaa acgaaataac ctcacttatc tttcgtgggc atgggcttgg
      121 gcagaagtta gcaaagtgtg tgaggcagta gattatgaaa tctatcatga tccagaaaca
      181 tatctgccgt atgtctttga taagaaaaca ggctatatgg tttttaccag tatcacagtc
      241 aacggagtaa agcgtgacat gtggttacca gtcatggatg gtgcaaacaa ggcaatgaaa
      301 gatgagccat atacctacga agtcaatgat tatcagtgga ataacgaaac gaagaaaaaa
      361 gagattgttg gaaaaatcga aaagcgagtt gaagcagcaa ctatgtttga tatcaacaaa
      421 acgatcatgc gctgtcttgt aaaaaatcta gcaatgtttg ggctagggct atatatattt
      481 gctggcgaag atatgccaga agatgtctcg atgcttgaac cagctactca aagaagcaaa
      541 aagctattct tggatgcttt acaactggtt gctaacaagt acgaaaaatc aattgatgaa
      601 gcaattgttg cattgactga tgcggcttct ataaccgctg atgacagtaa atggaccaag
      661 agagacttgg gcattctaaa acgaggcgtt aactggattg aagatcagta cagagaagaa
      721 acaaaagaga agtgatatga gtgtttaaac cattgatcga ttcatattca gcggttctga
      781 aaaagttcaa aggaaaagac ataggtgcaa cgatcaatga agaagtgaac attgatcgac
      841 taaagacgat gtatgacgga tatgatggcg atcggattat tgaagttcga tttattgatc
      901 cacgtcgatt tactgtgcag caacgaaact tcatctatgc gctgataggc gatattttca
      961 tcgatacagg catgccaacg gacttctgga aggaat
//
LOCUS       DBNICI010000292          949 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.304, whole genome shotgun sequence.
ACCESSION   DBNICI010000292 DBNICI010000000
VERSION     DBNICI010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.304"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            28..>949
                     /locus_tag="AC1ZCC_002871"
     CDS             28..>949
                     /locus_tag="AC1ZCC_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HIQ1631068.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFL"
BASE COUNT          335 a          175 c          180 g          259 t
ORIGIN      
        1 cgaaagaatt ataaggagtg agtacaaatg acctataccc atcttacacc aactgaactc
       61 gtaatgatag aagcttattt caatcaatca cactctgttt caaaagtagc ccatctggta
      121 caacgttctc gacaaaccat ttataatgtc tatcgttttc ttaaatctgg tggaagcgca
      181 ataagctact atgaacaata taagaaaaac aaacgtaact gcggaagacg tccgattgtg
      241 ttaccagatg ggcaacaaga atacattcaa aaaaaggtgg ctcaagggtg gactccagat
      301 gtccttattg gtcgagcaga gtttcctatt gcttgttcgg tacgtacact atatcggatg
      361 tttaaagata aaacctttga ccctcacgat ttaccaatga aagggaaaag aaaaccaaac
      421 ggacataaag aaaaaagagg aaaacaagct tttcgccgtt cgattcagga tagaacaact
      481 gattatatac agttcaatca tgagtttggt cacctagaag gtgacaccat tgtcggtgga
      541 aaacataaaa gtgcggtgat cacgttagtt gagcgattat ctaaagtgat catcaccttg
      601 aaacccgctg gtaggcaagc tatagatatt gagaatagac tgaatcaatg gtttcaatct
      661 gtaccgaaac acttgtttaa atccatcaca tttgattgtg gaaaagagtt ctcgaattgg
      721 aaaaagatta gtaataccaa tgatatttcg atttatttcg ctgatccagg aacaccttct
      781 caacgaggac taaatgaaca ttcgaatggc ttattacgta aagatggctt atataaagct
      841 atggatttta ataaggtatc cgaaaccttt atccaatcgg tggcatcaaa acgaaatcat
      901 attcctagaa aatcattaca ctatcgcaca ccattggaag tatttttga
//
LOCUS       DBNICI010000293          945 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.305, whole genome shotgun sequence.
ACCESSION   DBNICI010000293 DBNICI010000000
VERSION     DBNICI010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.305"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(17..835)
                     /gene="purR"
                     /locus_tag="AC1ZCC_002872"
     CDS             complement(17..835)
                     /gene="purR"
                     /locus_tag="AC1ZCC_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HIQ1631069.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
BASE COUNT          276 a          201 c          157 g          311 t
ORIGIN      
        1 gctttttcac aacctcttat tctgaaaaat aatttcctgg cacaaccgta atatttttcg
       61 tttgagtatc tacatctttg acataaagca gtgacgtata atcatcgatg gcgcgatgac
      121 cgttgaattt tgattcggca aatacagtga tgccaacaag ttctgattcg aattcttcaa
      181 tcaagctctt cattccgttg actgttccgc cgcctttcat gaaatcatcc actacaagga
      241 cacgtgaacc acgtttcaaa cttctcttag acagttccat cttttcaatt ctttcagaag
      301 atccagacac atagttgaca cttactgttg atccttctgt tatttttgag tcccggcgta
      361 caataacgaa tggcgcattc agataatagg acacagcttg ggcaataggt acgccttttg
      421 tggctacagt cattacagca tcgatttttt caccaaggta tttagaagca atgattcttc
      481 caacttgacg cagcagttca ggttctccta acaaatcaga gagataaaca tatcctcctg
      541 gaagcagacg gtcttgttct gatagacgtt ctgctagaga ttcaatatat tttttcgcct
      601 cttcatatga aatttcaggg atgaacaata caccacctgc agcacccggg attgtttcca
      661 aggttccata ccctcgttct ttaaatgttt tcttgatgat tcccaaatct tcactgatag
      721 aagatttagc agattcataa cgttcggcaa agcttgttaa ggaaatcaat tcatgcgggt
      781 gattcaataa atactgtgtc atgtcaacta gacgttcact gcgacgtact ttcacctatt
      841 ccaactcctt ttaagaaatt ttatcgtttt acattatata agtttttctt caaaaattcg
      901 agcattttat gaaaaaataa gaaaatagtt cggtatttat taaaa
//
LOCUS       DBNICI010000294          923 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.306, whole genome shotgun sequence.
ACCESSION   DBNICI010000294 DBNICI010000000
VERSION     DBNICI010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.306"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            248..781
                     /locus_tag="AC1ZCC_002873"
     CDS             248..781
                     /locus_tag="AC1ZCC_002873"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIQ1631070.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          300 a          174 c          164 g          285 t
ORIGIN      
        1 gttgtgaaaa agtcgcctac tttcgagtcg taagaaattt ttttgaaaat ggttcctcac
       61 aactttgcat aaatttgact taataccgag aagtcatgat ccccatctaa ctccaaatca
      121 caaggaatga gttcaaaaac tagcttttct caacaaatat tacataaaaa attctatgtt
      181 ttttgttaaa tttatttgct tttttcaaaa gtatgttaca gtaatttccc aaggaggttt
      241 tacagaaatg aattttgttg ccatggattt tgaaactgcc aatcatcagc cttatagtgc
      301 ttgttctctt gcgctagtca tggtaaaaaa tagccagatc gttgatgaat tctatacatt
      361 gatacagcct gagactcctt ttttctggcg aaatgtccaa attcacggaa tccatcaaga
      421 agacgtacgc aatgcgccga aattcccaga agtctggcaa actatccaac catattttca
      481 aaaaaatcgt ttagtcgttg cacataatgc tgcttttgat acaaaggtct tagcgggatg
      541 cttagattat tatcatcttg aacagcctaa ttatctttct ttatgtacag tgaaaagtag
      601 ccgtcgcctg tttcctgaat ttccaaatca tcgattgaac actgtgtgcg aaaatttgaa
      661 catccaatta gatcatcacc atgatgcatt ggaagatagt cgtgcatgtg cggaaatcct
      721 gctttatcaa gaaaaaaaat tcgggacaga tccattgaaa aaacttgtga ctataaagta
      781 aaagaatact tatgaaacac acaaagaggt cgtgagcctg ccgtttcact ccgagtcaca
      841 aggagcaatt tcaaaaaata gcttctcatg tttattaaat attgtgactg gtgaccgagg
      901 agctggcagg agaacaccgt tta
//
LOCUS       DBNICI010000295          880 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.307, whole genome shotgun sequence.
ACCESSION   DBNICI010000295 DBNICI010000000
VERSION     DBNICI010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.307"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            369..880
                     /locus_tag="AC1ZCC_002874"
                     /pseudo
     CDS             369..880
                     /locus_tag="AC1ZCC_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          308 a          156 c          160 g          256 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact
//
LOCUS       DBNICI010000296          876 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.308, whole genome shotgun sequence.
ACCESSION   DBNICI010000296 DBNICI010000000
VERSION     DBNICI010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.308"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(555..869)
                     /locus_tag="AC1ZCC_002875"
     CDS             complement(555..869)
                     /locus_tag="AC1ZCC_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631071.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          333 a          127 c          129 g          287 t
ORIGIN      
        1 tggttgttgc cacataaagc cataaacaat caaccagtag aatgcgtggc aacaaacctg
       61 ttatcgcata tcttggagtg tgactattta tgagtgatag tgaagatatg cgtctccata
      121 tatgtatggt tgagacagat aaacatgtat gaaaaacgtt gttaaatcaa cgttttttta
      181 ttttggtcaa ggagagaaaa gtagagaaat acaaaatttt ttgcattgat tttgcattca
      241 aaaaaacatt caaacattta gaaatcccca atacgtattc aggaaaatct attttaaggt
      301 ttacaaaaag aacttttgtt cgtacactct ttttgaggtg aacttttatg ctgatggaag
      361 gaaaaacaca attatggttt aagcatgttc cttcaaataa gttttttaat tataaactct
      421 agagaaatta attcatccta acataaaata attgttcact caaaagtttt tctagaacaa
      481 ttaaaacagc aggcttcaca ttcgctatac atttgtaaga agtaataatt cctgtactaa
      541 aaggcataat tgctttaatt attttttgac ttctcttcaa acacaagaag tagttgtgta
      601 ataattgcag aaataaggat tccaataata gatataaaat ttgacatgga aacgtctata
      661 acattaaaag aataaagtgt tgaagaaagt aaactgattg aaataacccc tccgtaaaaa
      721 atagtctgtg aatataccca ctttttttca gaagacgtac ttcgtttggt tctaaaacct
      781 atccctgcat ttctactctt atcaaaaaac caaatattta gaagtaataa tattgctatt
      841 attaagataa aattactgta aaaatccaaa agctat
//
LOCUS       DBNICI010000297          876 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.309, whole genome shotgun sequence.
ACCESSION   DBNICI010000297 DBNICI010000000
VERSION     DBNICI010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.309"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..434)
                     /locus_tag="AC1ZCC_002876"
     CDS             complement(<1..434)
                     /locus_tag="AC1ZCC_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1631072.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
     gene            complement(451..855)
                     /gene="tnpA"
                     /locus_tag="AC1ZCC_002877"
     CDS             complement(451..855)
                     /gene="tnpA"
                     /locus_tag="AC1ZCC_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HIQ1631073.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          263 a          143 c          137 g          333 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc accagttgtc agaagacaaa aacttttaga
      541 ccagaacatt tctttccaaa gaaattgttt gactcttgga aagtcacgtt ttatcaatct
      601 tgagctggca cttttatagg cattgatgaa ttttgtcaat tctgttttag gttgagcttt
      661 gaacataata tgaacgtggt ctttatcatg attccactcc actaaagtaa tgtgatacga
      721 ttctgaaatt ctttcaaaag tagttttggc ataatcagat atttcatcat caatcacttg
      781 tctacgatat ttcgttacta aaacaaggtg gtaataaaga agaaatactg aatgattatt
      841 tgtatctaat tccatgttat atcaactcat ttcttg
//
LOCUS       DBNICI010000298          850 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.310, whole genome shotgun sequence.
ACCESSION   DBNICI010000298 DBNICI010000000
VERSION     DBNICI010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.310"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..117
                     /locus_tag="AC1ZCC_002878"
     CDS             <1..117
                     /locus_tag="AC1ZCC_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HIQ1631074.1"
                     /translation="RIHHTEKHKIGVTNFKNKYQIKGIKLNQETIKKLRIGG"
     gene            120..347
                     /locus_tag="AC1ZCC_002879"
     CDS             120..347
                     /locus_tag="AC1ZCC_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631075.1"
                     /translation="MINLNAVAFEQTWRTKYKKMSPRDKLFLEIMTFAFIGTQAEQSD
                     ISVEKIKTNRLVNGITETCYQYTIIVVDEEE"
     gene            365..>850
                     /locus_tag="AC1ZCC_002880"
     CDS             365..>850
                     /locus_tag="AC1ZCC_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replisome organizer"
                     /protein_id="HIQ1631076.1"
                     /translation="MFAKTIIDSDAFLDMPLSTQALYFHLSMRADDDGFINNPKKIQR
                     MVGCGDDDLKLLMAKRFILVFESGVIVIKHWKIHNYIRNDRYKPTLYQDEKALLADKD
                     NKAYTFAEELSKHDEKLGIPDDNQAVYQMDTQVRLGKDRLGKDSKEIKDLTPSKKSKA
                     KP"
BASE COUNT          328 a          125 c          173 g          224 t
ORIGIN      
        1 agaattcatc acacggagaa acacaaaata ggtgtgacca atttcaaaaa taagtatcaa
       61 atcaaaggga ttaagttaaa ccaggagaca atcaagaaat taagaatcgg gggctaatga
      121 tgattaattt aaacgcagta gcgtttgaac aaacatggcg cactaagtat aaaaaaatga
      181 gtccaagaga caaattgttc ttagaaatca tgacatttgc tttcattggt acacaagctg
      241 aacaaagcga tattagtgtt gaaaaaatta agacgaatag attagttaat ggaattacag
      301 agacttgtta ccagtacacg attatcgtcg tggatgagga ggaataattt tggcagaaag
      361 aagaatgttc gcaaagacaa ttattgattc tgatgcattc ctagatatgc ctttatcgac
      421 acaggcatta tatttccatc tatcaatgcg cgcagatgat gatggattta ttaataatcc
      481 caaaaaaata caacgtatgg ttggttgtgg tgatgatgat ttaaaactgc ttatggcaaa
      541 aagattcatc ttagttttcg agagcggtgt gatcgtcatt aagcattgga aaatacacaa
      601 ctatattcgt aatgatcgtt acaaacccac gttatatcag gatgaaaaag ctttactagc
      661 agacaaagat aacaaagctt atacatttgc agaagagctt tctaaacatg atgaaaaact
      721 tggtatacca gatgacaacc aagcggtata ccaaatggat acacaggtta ggttaggtaa
      781 ggataggtta ggtaaggata gtaaagagat aaaagattta acgccttcga aaaaatcgaa
      841 ggctaagccc
//
LOCUS       DBNICI010000299          849 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.311, whole genome shotgun sequence.
ACCESSION   DBNICI010000299 DBNICI010000000
VERSION     DBNICI010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.311"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..720
                     /locus_tag="AC1ZCC_002881"
     CDS             <1..720
                     /locus_tag="AC1ZCC_002881"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HIQ1631077.1"
                     /translation="GKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFT
                     NRLGTNKRGRLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVY
                     LANCYTLEGRKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITL
                     EEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNK
                     VQLIRLNKYRVLAGNQELNLI"
BASE COUNT          326 a          133 c          167 g          223 t
ORIGIN      
        1 gggaaaacgg aattagaaga aacaagactt tataattcaa tggtattagg tatgcaaaat
       61 tattatcaga ttgcaacgaa tgtaaatctg gattgtatga aactgaatag agctgtaatg
      121 gctatcttca ctaacagatt aggtacaaac aaaaggggca gattgagaaa aacaggcaga
      181 caacttacga caaaggaggc aacaagatat ggtaaatcta aaatgcttcg gtatgttgct
      241 ggcgagggcg aacctatttt ccctgttgga tatatccaac acagaaaacc aatggcaaag
      301 gtgtatttag cgaattgcta cactctagag ggaagaaaat ttatccacac caatctaagt
      361 gttgataaat tactgatgta ccagttaatg aaattatctt tggaaaatcg cacaatagaa
      421 tacgcagaca atcgaatttc gctattttct gctcaacatg gaaaatgtgc tataactctg
      481 gaagaatttc aacaagctaa tgaaatacat tgtcatcata ttgtaccaac tggagctggt
      541 ggaaatgacg attataaaaa tcttattttg gtaaaagagg cggtacaccg tctaatacac
      601 gctaaaacag aagaaacaat acaaaaatat atagctctat tgaagctaaa taaagtccag
      661 ctcataagac taaataaata tagagtatta gctggcaatc aagaattaaa cttgatatag
      721 gtctactaca taaatacttt tcaatataaa cacgatggaa cgcggagtgc ggtgaaagtc
      781 gcacgctccg tgtgaatggg gggaaaagtt tgagatattt ataatatcag gaacttacct
      841 atccatata
//
LOCUS       DBNICI010000300          824 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.312, whole genome shotgun sequence.
ACCESSION   DBNICI010000300 DBNICI010000000
VERSION     DBNICI010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.312"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(48..419)
                     /locus_tag="AC1ZCC_002882"
     CDS             complement(48..419)
                     /locus_tag="AC1ZCC_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631078.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(421..822)
                     /locus_tag="AC1ZCC_002883"
     CDS             complement(421..822)
                     /locus_tag="AC1ZCC_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631079.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
BASE COUNT          271 a          142 c          102 g          309 t
ORIGIN      
        1 tacaaagatt aaataaaaaa ataacaaaca aactcgcttg aaattcatta ggtgagtttg
       61 tttattattt tttctgcttc tcgattcatc agcgccatat ctgttttcgt aatggtttca
      121 ttaggaatat agtcagcaca ttttcttttc tctaacattc tatacccact attatgtatt
      181 cctaaggtat tcaattcctt aactgttgaa aacaaattcc aaacggtttg gtggcttcct
      241 tttccgctac ttccattata aataatatca taattttctt tcaaatactc atcagatttt
      301 ttaaaagcac tgtagtatgc tcttgaaaca gcagttcttc tataagattc tttagaatta
      361 tatttatcat ctgattcaat ttgtttggaa atagataaat attcattaaa gtcaaacatt
      421 ttattccccc aaagttgcta ctacagattt ccccatatat ttatagtcag ataatacatt
      481 atcgagagta tccaaaacat cgtaatctga ggaaaacaat tcagatgttg ttttgataac
      541 aaagaaatac aagtttaaat cttcatcata ttttatattg atttcagctt ttctttttaa
      601 aaccgtattt atcacatcaa aaattttgcc atcattactt gaaattgtgt taatctcatt
      661 ttttgtagta attacaagtt gattatcatt tttagttttc ggagtagaac ttgcatccac
      721 aacaactgga gatgctagaa ttaccgatgc gcaagcaaaa atcgcagcaa aatttattaa
      781 gctaccactc cgatgtgtat ttgatggtac tatatcaagc atcc
//
LOCUS       DBNICI010000301          809 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.313, whole genome shotgun sequence.
ACCESSION   DBNICI010000301 DBNICI010000000
VERSION     DBNICI010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.313"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
BASE COUNT          309 a           95 c          105 g          300 t
ORIGIN      
        1 agcaatatat aaaagaaggt atcaagagtc acagtgaatt tattccgaaa agtgaaaaga
       61 gcaaatataa aatttaataa aaataagaac taaataggaa aacgtagacg taattttcta
      121 cgttttcctt ttcatatata ttaaatatta cggaaaatta tataaactaa tttagtgttt
      181 tgtttgtaat tcctaatttt gctccacttt tagataattt ttaggtctat ttaactcatt
      241 agacattcta tgctcatttt tctccaaatt tgtttcaact ctttcgtact taattttgcg
      301 tatttgaaaa ttcttctttt agagtacttt tattacgata ctagtatgat atatatctac
      361 agcaaacagt taaaatcttc ctaaaataag cgaatagcat tcctaaaacc tataaaattt
      421 ttagatgaag cgacaagtgg acaattaata aactacttga acatgaaatt atggatcacc
      481 tttcaaattt agaaaatgga attcttatac ttattgcgca acatttattg attgcaaaat
      541 tatgtgacaa aaagaactag tagaagaagg aataatggct tgataataaa gtactaccta
      601 ttaaaggtag tactttattt aatttagtat aaattaacat actactatat tttaggtttc
      661 atattatata aattaataat atactaccat ttgttggtag tataatttaa aggtaataag
      721 ttattctttt attactatgt ttgggtttta tagcaaagaa agtttaataa gagtttggac
      781 ctagcaaaca atattaatga ggtcattaa
//
LOCUS       DBNICI010000302          808 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.314, whole genome shotgun sequence.
ACCESSION   DBNICI010000302 DBNICI010000000
VERSION     DBNICI010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.314"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            69..266
                     /locus_tag="AC1ZCC_002884"
     CDS             69..266
                     /locus_tag="AC1ZCC_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HIQ1631080.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            268..579
                     /locus_tag="AC1ZCC_002885"
     CDS             268..579
                     /locus_tag="AC1ZCC_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HIQ1631081.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            683..>808
                     /locus_tag="AC1ZCC_002886"
     CDS             683..>808
                     /locus_tag="AC1ZCC_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HIQ1631082.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
BASE COUNT          323 a           77 c          148 g          260 t
ORIGIN      
        1 ttgttttttg aaatttaatg aatgttagaa taatagtcta aaaacattaa tttaggggtg
       61 attagattat gaaacattta aaaattttgt ctattaaaga gacacaactt atctatgggg
      121 gtaccactca tagtggaaaa tattatggaa atggagtgta ttgcactaaa aataaatgta
      181 cggtcgattg ggccaaggca actacttgta ttgcaggaat gtctataggt ggttttttag
      241 gtggagcaat tccagggaag tgctaaaatg aaaaaaaatg ctaagcaaat tgttcatgaa
      301 ttatataatg atatatctat aagtaaagat cctaaatatt ctgatattct tgaggtttta
      361 caaaaggtat atttaaaatt agaaaaacaa aaatatgaat tagatcccag tcctttaata
      421 aatagattgg tgaattatct atattttact gcttatacta ataaaataag attcactgaa
      481 tatcaagagg aattaataag aaatttgagt gaaattggaa gaactgctgg aataaatggt
      541 ttatatcgag cagattatgg agataaatct caattttaat gatacttata atattaaata
      601 tgtctcaaaa ttttttgatt agaaaaatag gtatattgaa tcagtgacat aaaagtattt
      661 atagtttgta gggggaatcg taatggaaga aaaaaataga ctaaatgcta aacaatgttc
      721 agatcaagag cttaaaaaaa ttaaaggagg tgctggaaca aaacctcaag gaaaaccggc
      781 atcaaattta gtggaatgtg ttttttca
//
LOCUS       DBNICI010000303          790 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.315, whole genome shotgun sequence.
ACCESSION   DBNICI010000303 DBNICI010000000
VERSION     DBNICI010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.315"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..716
                     /locus_tag="AC1ZCC_002887"
     CDS             <1..716
                     /locus_tag="AC1ZCC_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HIQ1631083.1"
                     /translation="KKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTST
                     ESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADK
                     SAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWS
                     ESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNE
                     TANNELKNGFNQIVYGQAN"
BASE COUNT          282 a          151 c          159 g          198 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 cttttatttt
//
LOCUS       DBNICI010000304          789 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.316, whole genome shotgun sequence.
ACCESSION   DBNICI010000304 DBNICI010000000
VERSION     DBNICI010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.316"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..326)
                     /locus_tag="AC1ZCC_002888"
     CDS             complement(<1..326)
                     /locus_tag="AC1ZCC_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631084.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNI
                     CTILIS"
     gene            472..>789
                     /locus_tag="AC1ZCC_002889"
     CDS             472..>789
                     /locus_tag="AC1ZCC_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631085.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLT"
BASE COUNT          288 a          104 c           95 g          302 t
ORIGIN      
        1 aaactaatca gaatcgtaca aatatttaat aaccaaatat atagaacata acttaatatt
       61 aagttatttg aaattccata aaaaacgact gcaggaatta ttgtaagaaa aggataaaaa
      121 tagttcagtc catctccata attgttaaat gttgtatagt tgattggccc ttcaaaaata
      181 gaagataacc ctttgattcg tgaaagatga aaagataaat cacctgttgt ataaatttct
      241 tgagcatttc tcataaaatt tactgaatat aaagtactaa gtaagataag aactactaaa
      301 agaaatgctt ttttacttat tttcatagat ttgttgttct ccttttttaa ttctattttc
      361 agtatactag aattgtcttt ttttaataag ttttgcgtta aatttttcat agcactagta
      421 tgatatacta gaaaagaatc aatttttatt acttttaaat aggagaaaaa aatgaaaaaa
      481 aacaaaaacc aaataataga tatcattttt atgttatttc taagttttgc ttacgtcatc
      541 cctctattca tgtctatggg aatctatcat agtgtcaatc aggatactta ctttcattta
      601 tctcgaatca taggactaga taatgtatgg agttctccag taaacttcaa taacttcgat
      661 catcatggaa caatgatgaa tattttttat ccatggctta ctctttatcc agctttttta
      721 ttttataaaa tgatgggaaa tcttgtttta agttataata tctactattt ttttatcact
      781 tttttaaca
//
LOCUS       DBNICI010000305          784 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.317, whole genome shotgun sequence.
ACCESSION   DBNICI010000305 DBNICI010000000
VERSION     DBNICI010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.317"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..>784
                     /locus_tag="AC1ZCC_002890"
     CDS             <1..>784
                     /locus_tag="AC1ZCC_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIQ1631086.1"
                     /translation="EISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGIS
                     SRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHL
                     LFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDF
                     IHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERL
                     KNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT          240 a          136 c          171 g          237 t
ORIGIN      
        1 tgaaatcagt atcctcaact tgacgagtaa gtcagaaaat caaggtctgc ttgatcctta
       61 tgtgattatg aagaaaaaga aagactcgga aagtttagca attgatattc tgactttttt
      121 aacaggaatt tcaagtcgag acggggagaa gtttcctgtg ttacgtaaag ccattcgaac
      181 ggtgactcaa agtgatcaac gggggttatt acaagttatt aacgaattgc gaaaagacgg
      241 gtcaccaatc gcggaaaata tcgccgatca tatcgagtcc tttaccgatt atgattttgc
      301 tcatttgctt ttctcagacg gctcgattac ccaatcgatt agtttagata aacagctaaa
      361 tatcattcaa gtggcggatt tggttttacc ggatgcagaa acgacttttg aagaatatac
      421 caccatggaa ttgcttagtg tttcgatgct aatcgtcatt tccacttttg ccttggactt
      481 tatccatagt aaccgagaaa tcttcaaaat tgtcgatttg gatgaggctt ggagttttct
      541 tcaagtggct caaggaaaaa cgttatcgaa taagcttgtt cgtgctggtc gggccatgaa
      601 tgctggggtc tatttcgtca ctcaaaatag cgacgattta ttagatgaac gtttaaaaaa
      661 taatatcggg ttgaagtttg cttttcgcag taccgatatt catgaaatca agaaaacctt
      721 ggaattcttt ggtttggatc aggaggatca aagcaatcaa aaacgcctgc gagatttgga
      781 aaac
//
LOCUS       DBNICI010000306          782 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.318, whole genome shotgun sequence.
ACCESSION   DBNICI010000306 DBNICI010000000
VERSION     DBNICI010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.318"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..>782
                     /locus_tag="AC1ZCC_002891"
     CDS             <1..>782
                     /locus_tag="AC1ZCC_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIQ1631087.1"
                     /translation="AKQNAQRTNVKKSTQKQREQLMRLLKEDKLGARSIDTIKPSDAK
                     EWALRMKDKGFSYNTINNHKRSLKASFYIAIQDDYVRKNPFDFKLSEVIENDTKEKVA
                     LTEEQEQALLSFIKTDNVYHKHYDDVLILLKTGLRISELCGLTVADIDFKNEVVIIDH
                     QLLKSKEQGYYIETPKTKSGIRQVPLSKETIQAFQRVINSRPKIKSIEVDGHSGFVFI
                     NPKGNPKVAIDYNALFIRMVKKYNKHHKDNPLPHITPHTLRHT"
BASE COUNT          290 a          154 c          167 g          171 t
ORIGIN      
        1 gccaaacaga acgcacagag gacaaatgtg aagaaaagca cacagaaaca acgggaacaa
       61 ctcatgcggt tattaaaaga ggacaagtta ggtgctagga gcattgatac gataaaaccc
      121 tctgacgcta aagaatgggc gttacgcatg aaagacaaag gcttttccta caataccatt
      181 aacaaccata aacgctcgtt aaaagcgtca ttctacatag ccatacaaga tgattatgtg
      241 aggaaaaacc cttttgattt caagctaagt gaagtcatag aaaatgatac caaagagaaa
      301 gtcgcattga cagaggaaca ggagcaagcc ttattgtcat ttattaagac ggacaacgtg
      361 tatcacaagc attatgatga tgtgctgata ctgttaaaga caggacttcg tatctcggaa
      421 ctgtgcggac tgacagtagc cgacattgat ttcaagaatg aagttgtgat tatcgaccac
      481 cagttactaa agagtaagga acagggctat tatattgaaa cgcctaagac gaaaagcggg
      541 ataaggcaag tgccattaag caaagaaacg atacaggcat ttcaacgggt tatcaacagc
      601 cgaccaaaga taaagtcgat agaggtagac ggacacagcg gttttgtgtt tatcaatccg
      661 aaaggtaatc caaaagttgc cattgattat aacgccttat ttatccgtat ggtaaagaaa
      721 tacaacaagc accacaagga caatcccttg ccacatatta caccgcatac gctacgccat
      781 ac
//
LOCUS       DBNICI010000307          768 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.319, whole genome shotgun sequence.
ACCESSION   DBNICI010000307 DBNICI010000000
VERSION     DBNICI010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.319"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..582)
                     /locus_tag="AC1ZCC_002892"
     CDS             complement(<1..582)
                     /locus_tag="AC1ZCC_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631088.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNS
                     LDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYK"
BASE COUNT          247 a          127 c           91 g          303 t
ORIGIN      
        1 tttgtaaaaa tattcgattg aatattctat ttggcacgac tcacttggca gtatatgtat
       61 gtcaatattt ttcatctgtt tcataggtat ttcaaaatct aaactattaa aaacatataa
      121 ttccctgtaa ttatttacat ctaactctac taaatttatc tttggaacaa tagatcctcc
      181 acccattcca ccataataaa aaaaatcttg ttctctatca aaatagcaca tattaagctc
      241 agtttgttta tcatcctcaa taattttata aatcaaacct atataatcat gaatgctggc
      301 aaaaattttt tttaagtcaa gttctaaatc ttcttcgaaa acacatttaa tttctcctac
      361 tgataattga tactctatat tacatgactt aattagtttt ttagatcccc cctccagata
      421 ggcatatgtt tcaataatta tgttcttttt attgcttggc aaatttccgt tatttattgc
      481 ataaattaaa aatgactgtc tatttagccc ataatctaat acaacttctt catactcata
      541 tttctttaaa tatttaatat gaaaatatgc tgaattaatc aaaactcgag tgttactgtt
      601 gttatttatt gttgttctat tgtgtataaa attgtatagg ttgtcatatt ttcttggttt
      661 agtgaactac tcaccactta gctaaaccta acggtttctt acgtttgaag tgggagcttc
      721 ttgggaagag agcatacttg atagcgtatt actttactaa cagaggtg
//
LOCUS       DBNICI010000308          759 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.320, whole genome shotgun sequence.
ACCESSION   DBNICI010000308 DBNICI010000000
VERSION     DBNICI010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.320"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            192..>759
                     /locus_tag="AC1ZCC_002893"
     CDS             192..>759
                     /locus_tag="AC1ZCC_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HIQ1631089.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSN"
BASE COUNT          261 a          114 c          138 g          246 t
ORIGIN      
        1 aaggggctgt gacaagactt ttgtcacagc cccttattgt tgtactttta acctacaata
       61 ttttttgatt ccccctgagt actatttttt ataaatgcta gtaaatcaat cttctttggc
      121 ctttttttga taaaaaaaat actagatgta agataaaagt gtaaaaacga tttgttttag
      181 gaggggaaac gatgtgtacc gcaatcactt atgcaacgaa agaccattat tttggaagga
      241 attttgatta tgagatgtct tataatgaag tggtcactat tactccaaga aattatcgtt
      301 tcgattttag aaaggtaaaa aatttagata aacattatgc aatgatagga atagcagctg
      361 gggtatccaa ctatccactc tattatgagg ctacaaatga aaaagggttg agtatggcag
      421 gactgaattt tcctggcaat gctgattata aagaattaca agaaggaaaa gataacgttg
      481 caccatttga gtttattcca tggatactag ggcaatgctc aaccatagac gaagctaaag
      541 aattattggc tactatcaat ctagtcaata tcgattttag tgaaaaacta cccttgtcac
      601 ctttacactg gttattagct gataaagaaa aatcgatagt aattgaaagt atgaaagatg
      661 gacttcattt atatgataat cctgtcggtg tgttgacaaa taatccgcca tttgattatc
      721 aattatttaa tctgaacaat tatcgctcgc tatcaaatg
//
LOCUS       DBNICI010000309          742 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.321, whole genome shotgun sequence.
ACCESSION   DBNICI010000309 DBNICI010000000
VERSION     DBNICI010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.321"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..464)
                     /locus_tag="AC1ZCC_002894"
     CDS             complement(<1..464)
                     /locus_tag="AC1ZCC_002894"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0016019 - peptidoglycan immune receptor
                     activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045087 - innate immune response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein family
                     protein"
                     /protein_id="HIQ1631090.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGPNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPG"
     gene            complement(475..672)
                     /locus_tag="AC1ZCC_002895"
     CDS             complement(475..672)
                     /locus_tag="AC1ZCC_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HIQ1631091.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
BASE COUNT          198 a          151 c          127 g          266 t
ORIGIN      
        1 cctggttgat tatacggtgt gtctagtgta agtggaatac catatttcat tgctgaatct
       61 ctagctaatt caacgtatgc cttgtagttt ttctcaaacg ttgctttatc atgtgtgtgt
      121 tgtaactcaa tctgcacagg actatttgca ttagcatacg aaccagcacc gtactgtaca
      181 taaccaggtt gaccgacttg ataaacaatt ccgccgtctc ccacaatata agcagtataa
      241 gcgctagtcc atgaacgttg catatactgc gcttcattgc gtcctgttgc tgtttcatta
      301 gccgtttcat gcagtaaaat gtactgatta tttgctactt gtgagctacc ttcgtttggg
      361 cctaaattaa attcattgtt aatcgtatag gcaaacccat taattggcaa taaaaaaaga
      421 gccgttaata ggctcatcgc agtaatagta attttctttt tcatttgttt cctcctattt
      481 tttcaaatta taagccgaca cgccagtgat aacgcctaaa aatgttgcta ctgcattgat
      541 agtgagtact gtcatatctg ttccattcca tccatacgct ttgcctagtg ttgcaactaa
      601 tacagatgca gctggcaata ctgttaaaac tgtccattta atgacttgat aatacttatc
      661 gggtaaaatc atttcttctt aactcctttc ttttttaccg agatctttct ctaaataaag
      721 ttttgatttg ttgtgtgtgt tc
//
LOCUS       DBNICI010000310          697 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.323, whole genome shotgun sequence.
ACCESSION   DBNICI010000310 DBNICI010000000
VERSION     DBNICI010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.323"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..>697
                     /locus_tag="AC1ZCC_002896"
     CDS             <1..>697
                     /locus_tag="AC1ZCC_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HIQ1631092.1"
                     /translation="QKKKTLVVDAVLSPTPRACRSCGSTVVDGNGKAIIVKNGKKETI
                     VRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKV
                     SLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSKKILPRVLMVDEFRSHASIEDKMSFIC
                     ADGETGKLIDVLPTRKLPRLTSYFLGCTNPEEVEFLVTDMNAAYFQLTKRVLPNAKVV
                     IDRFHIVKHMNQA"
BASE COUNT          218 a          135 c          140 g          204 t
ORIGIN      
        1 ttcagaagaa aaagacgttg gtcgtcgatg ctgttctctc gcctactcct cgtgcttgta
       61 gaagttgtgg ttctactgtg gtagatggaa acgggaaagc aattatagtg aaaaatggaa
      121 aaaaggaaac gattgtccgt tttgaacaat acaatcatat gcctttggtt atgcgcctaa
      181 aaaagcaacg ctatacctgt aaaaactgtc gcacccattg gacggctcaa agttattttg
      241 tccaacccag acattcaatc gcaaatcatg ttagatataa aattgcttct ttactgactg
      301 aaaaagtatc tttatctttt attgcgaaaa gctgtcaggt atctttgacc accgttattc
      361 gtacattgaa agagtttaaa agctatttac caaagcaatc taagaagatc ctccccagag
      421 tattgatggt tgacgaattt cgttcgcatg cttccataga agataagatg agctttattt
      481 gcgcggatgg cgaaacagga aaattaatag atgttttgcc tacgcgtaaa ttacctcgat
      541 taacaagcta tttcttaggt tgtaccaatc cagaagaagt agaattcttg gtgacagaca
      601 tgaacgccgc ctacttccag ctcaccaaac gtgttctgcc aaatgcgaaa gtcgtgattg
      661 atcggtttca tattgtcaaa cacatgaatc aagcgtt
//
LOCUS       DBNICI010000311          684 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.324, whole genome shotgun sequence.
ACCESSION   DBNICI010000311 DBNICI010000000
VERSION     DBNICI010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.324"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            1..684
                     /locus_tag="AC1ZCC_002897"
                     /pseudo
     CDS             1..684
                     /locus_tag="AC1ZCC_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
BASE COUNT          251 a          123 c          145 g          165 t
ORIGIN      
        1 taagtatact tttgaactta aattaaagat cgttcatgat tacttggatg gtaaaggcgg
       61 aagtgattat ctggcaaaga aatattccat taaggctcca tcacaagtga agcgctggat
      121 caatgcctat caagaatttg gtgaagaggg tctagtaaga aagcgacaaa agaaaaaata
      181 ttctgttcaa tttaagctgg atgcgataga gttatatcta acaagtgagc tgtcctatcg
      241 tgaagttgcc aacacactta acatgaacaa ccctaatttg atcgctagtt ggatgcgtca
      301 attccgtgag ggtgggatcg atggactctc aaaaacgaaa ggatgtccac ctatcttgtc
      361 taagaaaaat gaacccaaaa ataagaaaaa atctacaact aaagcaacgt ctaaagaacg
      421 tgaacgcatc gaggagttgg agaaacgagt ccgttccctt caaattgaaa atgccttttt
      481 aaaagaattg aggaagctgc gaaaacagga agcccaacaa cgacgaatga agcaatcgca
      541 cgaatcatcg caagcctccg aggaccgttt aaattagttg agcttctaga aactttaaag
      601 ttccctaagg caatctttat gtactggcaa aaacgattgg accgtaaaaa tccagatcaa
      661 gagattgaag cggaaatgct taaa
//
LOCUS       DBNICI010000312          667 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.325, whole genome shotgun sequence.
ACCESSION   DBNICI010000312 DBNICI010000000
VERSION     DBNICI010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.325"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..196
                     /locus_tag="AC1ZCC_002898"
     CDS             <1..196
                     /locus_tag="AC1ZCC_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HIQ1631093.1"
                     /translation="SKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETA
                     IRMEIDQLNRQQRLFGDDNE"
     gene            374..>667
                     /locus_tag="AC1ZCC_002899"
     CDS             374..>667
                     /locus_tag="AC1ZCC_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631094.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYG"
BASE COUNT          242 a          113 c          137 g          175 t
ORIGIN      
        1 cagcaaaaaa gtattctcaa aattttggtc tgaatttaaa cgtacctttt caatcccaag
       61 atatggcgag ttacctcgta agagattcga tgatgctgtt tcatttattg aaatgtggtt
      121 gccagaaact gcgatccgta tggaaattga tcaactgaac agacaacaga gactttttgg
      181 tgatgacaat gaatagagct gaagcgctaa gaatagggac ggcaattgct aattgctggt
      241 ggaaatacta taaaccaatc atcctaagcc aacaacatat tgacaagcaa aaagcatggc
      301 aacaaataaa aaagtgactc cgccggcaag caaagagtca caaagaaaac acatcataag
      361 gagattttag catatggaaa aagaactttc cactctagat caatatttga ttgatcctga
      421 ttggggcaag ccgaaaattg aggaaacaag tggtcgaaaa atcagacgaa atcttttgac
      481 gaatgaagaa ctagcttggg atcaagatga tttaggcaac catgtaacta tttgggatca
      541 tgtttatctt atccatctat cgaagcattc gaataaacct gaatatattt acgtcatcga
      601 agatggcttg attgatgcgc tagaagagta cgacagagat aacttgattg atatctctta
      661 ttacgga
//
LOCUS       DBNICI010000313          651 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.326, whole genome shotgun sequence.
ACCESSION   DBNICI010000313 DBNICI010000000
VERSION     DBNICI010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.326"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..462)
                     /locus_tag="AC1ZCC_002900"
     CDS             complement(<1..462)
                     /locus_tag="AC1ZCC_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631095.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQR"
BASE COUNT          265 a           97 c          126 g          163 t
ORIGIN      
        1 cctttgaaga agcgcttcat aaactgactg gaagcttacg atccacaata aaaccaagat
       61 acaacaaaga aagagaggaa agaacagctg ccagaaaata aaaaggaata atttggtttt
      121 agaaagacca gtcaggtgcc aattgatcaa ttcctgcttg ttttgcctga ctgaatagta
      181 aaagaaaaag ataaaaagga aggcaaatag aaaaaataaa ctgaatatgc cttttatata
      241 aaatcggcta gctaattcta aagccggaag aagctctgta gatgcagaag cgccaataaa
      301 tgaaagccga tcttggactt gattggctaa agccgattgc aaatggtaaa gagtcaggca
      361 tgctatcaat aaaaaagcag aaaagcttga aaataaacaa tagagtattg tctgcttttt
      421 gtaataaatt aatcgagcag tagcatagcg gattattttc atacttttca cctctataaa
      481 aagtatagaa aatgaatatg aataaagtat gaaattcctt tagtgaaatc acgaattaaa
      541 accagaaaat attttgtaaa aagcaagagg ttgtgaagaa gctgccctat tctattaccg
      601 tagatgcaag cttttgaaca ctgtttattc gaaaagagga gcagcgacaa t
//
LOCUS       DBNICI010000314          630 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.327, whole genome shotgun sequence.
ACCESSION   DBNICI010000314 DBNICI010000000
VERSION     DBNICI010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.327"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..>630
                     /locus_tag="AC1ZCC_002901"
     rRNA            <1..>630
                     /locus_tag="AC1ZCC_002901"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          156 a          144 c          196 g          134 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga gatagagctt
      421 ccccttcggg ggcaaagtga caggtggtgc atggttgtcg tcagctcgtg tcgtgagatg
      481 ttgggttaag tcccgcaacg agcgcaaccc ttattgttag ttgccatcat tcagttgggc
      541 actctagcaa gactgccggt gacaaaccgg aggaaggtgg ggatgacgtc aaatcatcat
      601 gccccttatg acctgggcta cacacgtgct
//
LOCUS       DBNICI010000315          617 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.328, whole genome shotgun sequence.
ACCESSION   DBNICI010000315 DBNICI010000000
VERSION     DBNICI010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.328"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            46..>617
                     /locus_tag="AC1ZCC_002902"
     CDS             46..>617
                     /locus_tag="AC1ZCC_002902"
                     /inference="COORDINATES: protein motif:HMM:NF014537.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HIQ1631096.1"
                     /translation="MASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLDCG
                     GVIRRFDLAINDMCGLLDIPVLSEKYKNGGADCRCKNYENVQGGKLSGKNRNLASTLY
                     IGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHGLV
                     FYLITDFVQFPDYPLWEIFISHDSLPFEMNP"
BASE COUNT          191 a          113 c          139 g          174 t
ORIGIN      
        1 tattatggct ataaagagca ttacgcctac ggagaaatca aggttatggc ttctgatgat
       61 gaacacatgg gcgtattttt ggaattaaaa ggggcaggct cacgcaatat ggaatatgtt
      121 ttacaggcac agaacaggga ttggtattca ttcttgaacc gctgtcttga ctgtggcggt
      181 gttatccgac gttttgactt ggcaatcaac gatatgtgcg gtctgctgga tattcccgtt
      241 ttatctgaaa aatacaaaaa tggcggtgcg gattgtcgct gtaaaaacta tgaaaatgtg
      301 caaggcggta aactaagcgg aaaaaaccgc aatttagcaa gcacccttta tatcggctca
      361 aaagcaagca caaaatattt ctgcctgtat gaaaaacaaa aggaacaggc aactaaaaag
      421 aaacatacgg atattatcaa ccgttttgaa attcgtctgc gtgacaaaaa ggcagtacag
      481 gcagttgagg aactgttatt gacgtataac cctcatgggc tggtgtttta ccttattact
      541 gattttgtgc aatttcccga ctatccgctg tgggagatat tcatttccca tgatagttta
      601 ccttttgaaa tgaaccc
//
LOCUS       DBNICI010000316          599 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.329, whole genome shotgun sequence.
ACCESSION   DBNICI010000316 DBNICI010000000
VERSION     DBNICI010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.329"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     misc_binding    complement(131..358)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          192 a          118 c           78 g          211 t
ORIGIN      
        1 gcttctttga tcgattgcgg gcgtaagaaa cttccgacat gatctactgt aaatggtcta
       61 tttttgctca tttcactcac tcttttcttt gttttttgta aaaaaaaatc cttttactag
      121 caaatactag taaaaggacg attatactcg tggtaccacc ttttttcaga aaaaacattc
      181 tttctctcag cctgacaata aaatcagtgg tcttggtaac gaaagactgt tcgtttttgc
      241 ctacctttcg acaaaaaatc ctctaaggcc attttcgaca gtcaacttct agctttttac
      301 accaaccaaa gcctctcttg aaccattttc tgtctacttt ccttctcatc ggatactatt
      361 agaataaaat tagaatagca tgtttgaaag aaaagtcaat agggaataag aacaaacaaa
      421 aactaatttg ttattcttag taatttgttt gtggaataca ataaagcttt tctaatatga
      481 taattgttca gtcgtccttt catcttagat cacttcctta aaatgatcat acccgtttca
      541 accacataaa aaaggactta attttgaaaa atttcccatc aaaactaagt cctttttat
//
LOCUS       DBNICI010000317          585 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.330, whole genome shotgun sequence.
ACCESSION   DBNICI010000317 DBNICI010000000
VERSION     DBNICI010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.330"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..>585
                     /locus_tag="AC1ZCC_002903"
     CDS             <1..>585
                     /locus_tag="AC1ZCC_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HIQ1631097.1"
                     /translation="ALSKRGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKT
                     TNVLNRKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLV
                     KKAFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAEST
                     YKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHL"
BASE COUNT          204 a           97 c          108 g          176 t
ORIGIN      
        1 aagccttatc aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa
       61 atagaggatt aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca
      121 atgaagccaa aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag
      181 cgatcgtaac agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca
      241 ttttggatct gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg
      301 ttctagtaaa aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc
      361 atactgatcg tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg
      421 acatcaatcg atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa
      481 cttataagtc gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat
      541 tggatttgga gttatttgac tatgtcaatt ggtggaacca ccttc
//
LOCUS       DBNICI010000318          578 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.331, whole genome shotgun sequence.
ACCESSION   DBNICI010000318 DBNICI010000000
VERSION     DBNICI010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.331"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..389)
                     /locus_tag="AC1ZCC_002904"
     CDS             complement(<1..389)
                     /locus_tag="AC1ZCC_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HIQ1631098.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLG"
BASE COUNT          191 a          152 c           82 g          153 t
ORIGIN      
        1 cctaaatcac cagcttctac aatcaagtaa tacgctacat cactcatgtc attacaatcg
       61 ggataataga tgatatcatc cacatgctct accatttcct cgaaagaatt gaagaaggca
      121 tgttgaatct ctgaagcgac ctcaccaatt ggcgttcctt taagctcttc cgctaaacca
      181 cacaaccgat taatttcctc aattggtgta tactcatcaa tctcaaaagg cagttcataa
      241 tcatgaattg cgtactcttc atattcgtca tttaatccaa tccgttcttt cacttcatca
      301 aaatcgacgg gtggggtaaa ccatgccccc accaactcac cttcattgta ttttccaagg
      361 ttcgcaatat aaacacgtat ctgttccatt gtgattcccc ctcaggcacc gacaattcgg
      421 ttaaacaaat tgagtaagac atccttcaca ccagaagcat taaagaccag acctaccgca
      481 atcaaagcaa tcaccaagaa gccaatcagt ttggaaaact cgcgtttaaa tcctaaaaac
      541 aaaccaatga ccacaatcgc tagtaagact aaagattg
//
LOCUS       DBNICI010000319          576 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.332, whole genome shotgun sequence.
ACCESSION   DBNICI010000319 DBNICI010000000
VERSION     DBNICI010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.332"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            80..>576
                     /locus_tag="AC1ZCC_002905"
     CDS             80..>576
                     /locus_tag="AC1ZCC_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HIQ1631099.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          217 a          106 c           93 g          160 t
ORIGIN      
        1 aaaaatccat acaaaaaagg aagtcccttc tgtagaataa agttaacgac aaccaattca
       61 cagaaaagag gacttcccta tgaatgattt tactacagaa attgtgcaaa ctctagtcac
      121 taaaggcgat ttaaatgaat tattccgttc gcacttagaa aaagcgataa acacactcct
      181 acggactgaa ttaacggctt ttttagatta cgaaaaatat gatcgcactg gttttaattc
      241 aggtaattcg agaaacggtt cttactttcg atcaatcaaa accgaatatg gtgaattaac
      301 attggaaata cctagagatc gtaatggtga gtttaaacaa caaactttac cagcctacaa
      361 aagaacaaac gatacattgg aaaccactat tatccattta ttcgaaaaag gtgttacgat
      421 gtctgaaatt gctgatttga tcgaaaaaat gtacggtcat cactatactc cacaaaccat
      481 gtccaacatg actaaagttc tgactgaaga agtaaatgcc tttaaatcca gagccttaaa
      541 tgataagtat gtcgctattt ttatggacgc tactta
//
LOCUS       DBNICI010000320          565 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.333, whole genome shotgun sequence.
ACCESSION   DBNICI010000320 DBNICI010000000
VERSION     DBNICI010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.333"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..>565
                     /locus_tag="AC1ZCC_002906"
     CDS             <1..>565
                     /locus_tag="AC1ZCC_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HIQ1631100.1"
                     /translation="ALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIV
                     GRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKD
                     YSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFE
                     SVPKNLFKSITFDCGKEFSNWKQISNV"
BASE COUNT          195 a           97 c          112 g          161 t
ORIGIN      
        1 cagccttaga atattatcaa cagtataaga aaaacaaatc aaactgtggt agacgtccgc
       61 ttgttttacc cgaggaacaa tcagaatata ttcaaagaaa ggttgttcaa ggatggacac
      121 ccgatgtgat tgttggtcgt gcagcgtttc ctattagttg ttctgctcgt accatttatc
      181 gtatgttcaa aaaggggcta tttgattctt ctgacttacc gatgaaaggc aagcgtaaac
      241 cgaatggaca tcaagaaaga cgtggaaaac aaactttccg ccgctctatt catgaacgtg
      301 aaaaggatta tagccaattc tcaaatgagt ttggtcacct tgaaggtgac actatcgtag
      361 gtctgaaaca taaaagtgct gtaattacct tagttgaacg attatcaaaa gttatcatca
      421 cattgaaacc gtgtggtaga caagcgattg atattgaaaa aaaattaaat caatggtttg
      481 aatctgtacc gaaaaaccta ttcaaatcca tcacttttga ttgtggaaag gaattttcaa
      541 attggaaaca gatcagtaat gtcaa
//
LOCUS       DBNICI010000321          542 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.334, whole genome shotgun sequence.
ACCESSION   DBNICI010000321 DBNICI010000000
VERSION     DBNICI010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.334"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..>542
                     /locus_tag="AC1ZCC_002907"
     CDS             <1..>542
                     /locus_tag="AC1ZCC_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HIQ1631101.1"
                     /translation="TLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRK
                     TDYTQFDHEFGHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQW
                     FRCVPKNLFKSITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKD
                     GLYKAMDFNGVSETFIQSVAS"
BASE COUNT          198 a           94 c          108 g          142 t
ORIGIN      
        1 acactgtatc gaatgtttaa agataaaacc tttgacactc acgatttacc aatgaaggga
       61 aaaagaaaac caaacggtca taaagaaaaa agaggaaaac aagggtttcg ccggtcaatc
      121 cgagacagaa agacagacta tacccagttt gatcatgaat ttggtcacct ggaaggtgac
      181 actatcgtgg gtggaaaaca taaaagcgcg gtcatcacat tagttgaacg gctatctaaa
      241 gtcatcatta ccttgaaacc cgaaggcaga caggctaaag acattgaaaa taggctgaat
      301 caatggtttc gttgtgtccc aaaaaactta tttaaatcca tcacatttga ttgtggaaaa
      361 gaattctcaa attggaaaaa gattagtaat accaacgata tttcaattta ttttgcggat
      421 ccaggaacac cttctcaacg aggattgaat gaacattcaa atggcttatt acgtaaagat
      481 ggtttgtata aagctatgga ttttaatggt gtatctgaaa cctttatcca atcggtggca
      541 tc
//
LOCUS       DBNICI010000322          539 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.335, whole genome shotgun sequence.
ACCESSION   DBNICI010000322 DBNICI010000000
VERSION     DBNICI010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.335"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..114
                     /locus_tag="AC1ZCC_002908"
     CDS             <1..114
                     /locus_tag="AC1ZCC_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HIQ1631102.1"
                     /translation="IHHTEKHKIGVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            117..>539
                     /locus_tag="AC1ZCC_002909"
     CDS             117..>539
                     /locus_tag="AC1ZCC_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1631103.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKP"
BASE COUNT          227 a           81 c           98 g          133 t
ORIGIN      
        1 atccatcaca cagagaagca caaaataggt gtgattaatt tcaaaaataa atatcaaatc
       61 aaagggatca aattaaacca gaaaacaatc aaaaagttaa atattggagg gtaaaaatga
      121 cagaacatcg aagttattat gcgattatac cagccaacgt aaggtacgac aaaagactta
      181 aaccaaatac taagttgtta tacggagaga taacggcctt gtgtaatgaa agaggctttt
      241 gttgggcagg caatgagtac tttgcagatt tatatggtgt gaataaagag accatatcgc
      301 gatgggtaag tgatttgatt aagtttggat acttgaatcg ggaaatcatt tacaaagagg
      361 gtaccaatca aataatcaat aggtacctac gaattaatca ataccctatt gacgaaaaac
      421 gcaatacccc tattgacgaa aaagtcaaag ataataatac atctattaat aatacattta
      481 ataatacaaa agaatatata agagagttac cgccttcgaa aaaatcgaag gctaagccc
//
LOCUS       DBNICI010000323          531 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.336, whole genome shotgun sequence.
ACCESSION   DBNICI010000323 DBNICI010000000
VERSION     DBNICI010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.336"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            316..429
                     /locus_tag="AC1ZCC_002910"
     CDS             316..429
                     /locus_tag="AC1ZCC_002910"
                     /inference="COORDINATES: protein motif:HMM:NF020874.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HIQ1631104.1"
                     /translation="MGLNAISAKYCKFKAFVEKFTERQTDKIIQYHITYTD"
BASE COUNT          225 a           50 c           69 g          187 t
ORIGIN      
        1 ataataaaga attgaacttc tgagaaaaca aagaatcttt tttctttttt tgaattgact
       61 catggattgg cgttaaaatt ttattttgaa aaagttattt ctggtttaac tcaaaaaaag
      121 agatatgaat ctaatagtga tagaaaggat gttatttttc atgaaatggt tgttttagat
      181 ttaatacata taactaataa atgctattga atgtatttaa actaattaac aaaagaattt
      241 gaattgttaa taaaatccaa taaaatatat taataaagta gatagaaata aaaaaacaaa
      301 gactaaacat atataattgg attaaacgca atatctgcaa aatattgtaa atttaaagcc
      361 tttgtagaaa aatttactga aagacaaact gataagatta ttcagtatca tattacctat
      421 actgattaaa attttttgaa acgggatact aaataaaaaa tttatttata acagaactaa
      481 ttatttaatg cggatgcgcc aatacttggt cctgcaagag ctatttattt t
//
LOCUS       DBNICI010000324          515 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.337, whole genome shotgun sequence.
ACCESSION   DBNICI010000324 DBNICI010000000
VERSION     DBNICI010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.337"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(65..150)
                     /locus_tag="AC1ZCC_002911"
     tRNA            complement(65..150)
                     /locus_tag="AC1ZCC_002911"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="AC1ZCC_002912"
     tRNA            complement(167..238)
                     /locus_tag="AC1ZCC_002912"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="AC1ZCC_002913"
     tRNA            complement(250..322)
                     /locus_tag="AC1ZCC_002913"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="AC1ZCC_002914"
     tRNA            complement(340..421)
                     /locus_tag="AC1ZCC_002914"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="AC1ZCC_002915"
     tRNA            complement(429..501)
                     /locus_tag="AC1ZCC_002915"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
BASE COUNT          132 a          147 c          121 g          115 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaac
//
LOCUS       DBNICI010000325          514 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.338, whole genome shotgun sequence.
ACCESSION   DBNICI010000325 DBNICI010000000
VERSION     DBNICI010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.338"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            149..448
                     /locus_tag="AC1ZCC_002916"
     CDS             149..448
                     /locus_tag="AC1ZCC_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HIQ1631105.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
BASE COUNT          182 a           85 c          117 g          130 t
ORIGIN      
        1 cagatggcct gtatgaacca ccaatgaaat tcagaacttg gtcataaaat tggcgagaaa
       61 aaccttttgt cgccatttac ccagatggaa gaagaaagac gaataagcag aacgtgtatg
      121 ctctgtttta caaaaagggg agagatccat ggaacaatta gcttataaat atccaacgaa
      181 acagccgatc aataagaaaa tccatgtagg agtggtcggt tcaggagatt tggaaatctt
      241 gatgtttcca acagaaaaaa cagaatctac tgtaaaaatt tgtacaggaa gcgatggctt
      301 cggcgaagta tggaacaatg tattaacgag attttttgat cgttatccaa tttcagcaga
      361 tatcgtgatc aatgacttcg gtgctacacc aggagttgtg tacctacgat tgacgcaagc
      421 aatggaggaa ctttcagatg aaaaatagtt ttgtagaatt aaacgccaga gaacgagtga
      481 acgctctact tgacaatgga cgtgaattga ttga
//
LOCUS       DBNICI010000326          513 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.339, whole genome shotgun sequence.
ACCESSION   DBNICI010000326 DBNICI010000000
VERSION     DBNICI010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.339"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            7..195
                     /locus_tag="AC1ZCC_002917"
     CDS             7..195
                     /locus_tag="AC1ZCC_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631106.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            192..365
                     /locus_tag="AC1ZCC_002918"
     CDS             192..365
                     /locus_tag="AC1ZCC_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631107.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            334..462
                     /locus_tag="AC1ZCC_002919"
     CDS             334..462
                     /locus_tag="AC1ZCC_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631108.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
BASE COUNT          193 a           81 c          114 g          125 t
ORIGIN      
        1 agtgatatgg catatacctt gcaacaagaa catcaaattc tcggtttgat taaacaacgc
       61 aggaaacaat tacaagatga ccgtgcagcg cttagaaaag ccgatgagct atcagatagg
      121 caagctgaac taattgcttc tgaacttgag gatttgagaa tgctagaaat aaaaaatagg
      181 gagattagat tatgaagaag acagacacac tttttatagg attcattttg gggttattag
      241 tgattgtagc gcaccaaagt attatcgggg gaagcttgtt cgcagcattg atggttttaa
      301 tcaatctgct tgattcaaaa gaaaggagca actatggcac gagaagaagc gctaaaaatc
      361 ggtaaagtga ttgctgataa tcggtgggga aatagccgcc ctattatttt aagcaagcaa
      421 catatcgaaa agcaaaaagc atggcaacaa ataaaaaagt gactctgcca gccagcaaag
      481 agtcacatac aaatttataa aactaggaga att
//
LOCUS       DBNICI010000327          509 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.340, whole genome shotgun sequence.
ACCESSION   DBNICI010000327 DBNICI010000000
VERSION     DBNICI010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.340"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..321
                     /locus_tag="AC1ZCC_002920"
     CDS             <1..321
                     /locus_tag="AC1ZCC_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631109.1"
                     /translation="ENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEILLCLA
                     MQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVMTRILK
                     GEFQ"
     gene            318..485
                     /locus_tag="AC1ZCC_002921"
     CDS             318..485
                     /locus_tag="AC1ZCC_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631110.1"
                     /translation="MMNEKTIDLLIILFFTLVIVAKSYVIFGLLFAITMLVLCVLASR
                     KEVKHERVSKS"
BASE COUNT          178 a           74 c           98 g          159 t
ORIGIN      
        1 gaaaatctta tcgaagaatt gaaacgtgaa tgccaaaagc aaggtgtttc tattatttgt
       61 acagcccaaa aagaagggga acttaaaagt ctcgtttatg gtgagacaac agaaatttta
      121 ctttgtcttg ctatgcaaga agaacattta gatgaaaatc tcccattgtc agcacatatt
      181 atgcgtagaa ttgctgtgga tgcttacgaa caagcgaaaa atgaagaaga aaatcaacct
      241 tcaaatcata cttttgttat taataacaag gaagatttag ctgatgtgat gactcgtatt
      301 ctcaaggggg aatttcaatg atgaatgaaa aaacaattga tctactcatt attttatttt
      361 tcacgttagt gattgtagcg aaaagttatg taatctttgg cttgttattc gcaattacca
      421 tgcttgtgct ttgcgtgtta gcaagcagaa aagaggttaa acatgaacga gttagcaaat
      481 cttgacaact atttaactga tcctgatta
//
LOCUS       DBNICI010000328          487 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.341, whole genome shotgun sequence.
ACCESSION   DBNICI010000328 DBNICI010000000
VERSION     DBNICI010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.341"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(<1..>487)
                     /locus_tag="AC1ZCC_002922"
     CDS             complement(<1..>487)
                     /locus_tag="AC1ZCC_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIQ1631111.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DBNICI010000329          485 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.342, whole genome shotgun sequence.
ACCESSION   DBNICI010000329 DBNICI010000000
VERSION     DBNICI010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.342"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..249
                     /locus_tag="AC1ZCC_002923"
     CDS             <1..249
                     /locus_tag="AC1ZCC_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631112.1"
                     /translation="GKKIKKCDKCFESAVVKFPQFKLNPAASIQFQGHVFDVLAKDNI
                     KKYKKGNIFHIDIQISSNLEDIKKAFKIKTIGTAPNFK"
BASE COUNT          211 a           62 c           50 g          162 t
ORIGIN      
        1 ggtaaaaaaa taaaaaaatg tgacaagtgt tttgaatcag ctgtggtaaa gtttcctcaa
       61 tttaaactaa atccagctgc ctcgatacaa tttcaaggac atgtatttga tgttttagct
      121 aaagataaca taaaaaaata caaaaaaggt aatatattcc atattgatat acaaatatct
      181 tctaatttag aagatataaa aaaagccttt aaaattaaaa caattggcac tgctcctaat
      241 tttaaataac aaaaaaatta ctagataatc taaaaacagg atggcattat gagaacaata
      301 actaaacttc ctatatcaca tattttgtaa ttagtgaaaa gcaaattgac tactatcata
      361 agttaatgac tctcatctta atatttaaaa tatattcata tttttctatt ttatctaaat
      421 gcgattttca tatctaataa tagaaaaata aacaattaac aataaaaaag tcttcattta
      481 ttgtt
//
LOCUS       DBNICI010000330          477 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.343, whole genome shotgun sequence.
ACCESSION   DBNICI010000330 DBNICI010000000
VERSION     DBNICI010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.343"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(71..>477)
                     /locus_tag="AC1ZCC_002924"
     CDS             complement(71..>477)
                     /locus_tag="AC1ZCC_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HIQ1631113.1"
                     /translation="TPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMNA
                     ASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYEDS
                     QITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          150 a           95 c           72 g          160 t
ORIGIN      
        1 cacgacctaa agatcgtgtc taattatgac tgtatcagaa aatatgatac caaacggatc
       61 tattcattaa ttaattgaca tagtttatct gttgctcttt tatgactgga taactgacca
      121 attcatgacg atccaagtct tctttattca tatttacagc agtaatttga ctgtcttcat
      181 acgtacgata ataataaatc cctttatcaa cgttacagca agaagaataa atcgtatatt
      241 cgtattttcc ttctccagta tcgcatactc ctttttgttg ctcgactgag cctagtatat
      301 gaaaaaattg actgatactt tcagattctg aatcacctga tgcagcattc attttagtaa
      361 atgtggcttt tacaaatcga gaaacagaag agagatctcc aggtaagccc aatcctccca
      421 tcccacgact ataaacattc aagctgatct ggtttgaaaa atgattttct ggtgttc
//
LOCUS       DBNICI010000331          477 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.344, whole genome shotgun sequence.
ACCESSION   DBNICI010000331 DBNICI010000000
VERSION     DBNICI010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.344"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            192..407
                     /locus_tag="AC1ZCC_002925"
     CDS             192..407
                     /locus_tag="AC1ZCC_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631114.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
BASE COUNT          170 a           67 c           86 g          154 t
ORIGIN      
        1 ctcttttatc aagaattccg tggctttagt catgagaggc ttaatactat gaaaattttc
       61 tttagcattt ttgatttagg ttccttttct aattataata agatgaccac tacagtaacc
      121 tactgaaata tataaacagc cactgtagta ccattttcaa agtagtctat gagaggagaa
      181 tcttcattct tatgaacgct ggaattgcgg atatgaattt aataaaaaaa acccttaatg
      241 attttacttc caattcaata tcaaaaggta ctggtatcaa tttaagcaca ataaaaaagc
      301 tgaaaagtgg tgaaagatca gtggaaaagc tgaatctttt agatgctata aaaataacgg
      361 aatttgctat gaaaaatgga aaagctgaga ttgaaatatg gagataatta tcgtctagtg
      421 aattacttgc cacttagctg aatttaacga tccttggtgc ttgaaggggg agattct
//
LOCUS       DBNICI010000332          474 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.345, whole genome shotgun sequence.
ACCESSION   DBNICI010000332 DBNICI010000000
VERSION     DBNICI010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.345"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
BASE COUNT          153 a           64 c           67 g          190 t
ORIGIN      
        1 tcttgatgtg atatccttgt cttttttgta atgtttttaa cttattgctt ttgcaaaatt
       61 tatttacctg ttttctggat caacgatagt gtacattatg ctatccttgt ttggatttta
      121 ggacgtttaa agaggcactt ttttacataa cttaatccat agacctttta agcatgctgt
      181 gagataataa taaagaagag tttaaagcgc accccaaacc acttccccat aagtgtatta
      241 cgctttaaac tcttttatat ttgaattcac taagaagcat accatatttt tgaaaaaaag
      301 tgagaaaaaa ggcttataat taggatttag agtaattaat tagatgttta ttttatagga
      361 ctgatactat agattacaaa tagtatcgta tttcgcaatc ttaaaattat cttcttactg
      421 tgaataagat aataaaattt gtatttgtcc tttttaggta acttatttta atct
//
LOCUS       DBNICI010000333          464 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.346, whole genome shotgun sequence.
ACCESSION   DBNICI010000333 DBNICI010000000
VERSION     DBNICI010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.346"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            155..319
                     /locus_tag="AC1ZCC_002926"
     CDS             155..319
                     /locus_tag="AC1ZCC_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631115.1"
                     /translation="MDLKTFTAQIELMHQEALRQSASYEDKWLNTFHGGRESALDQVL
                     KLLKGERRDG"
BASE COUNT          174 a           78 c          106 g          106 t
ORIGIN      
        1 aatgatcgtt cgagttggag taaactcaga tactatttcg cactctatat cgatacaaca
       61 caattaagaa atttagctta tacaaaatta ctatttgttg attgcgttaa aggattgcaa
      121 aaacatctta gtgaacttga gcaggtgtaa tcagatggat ctaaaaacat ttacagcaca
      181 gatcgaacta atgcatcaag aagctttaag acaaagcgcc tcgtacgaag acaagtggct
      241 caacacgttc catggtggac gtgagagcgc acttgatcaa gtactcaaat tattgaaagg
      301 ggaacgtcgg gatggataag aaagcggcaa tgaaacgaat cattgaactg acgcattctg
      361 aaagttggca ggaagatgaa gaagtggttg ctgaagttca gcgactagga aagatcgcta
      421 atggacgtat acgaaaaaag ccagacaaaa gaaaacagcg caaa
//
LOCUS       DBNICI010000334          451 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.347, whole genome shotgun sequence.
ACCESSION   DBNICI010000334 DBNICI010000000
VERSION     DBNICI010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.347"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..419
                     /locus_tag="AC1ZCC_002927"
     CDS             <1..419
                     /locus_tag="AC1ZCC_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIQ1631116.1"
                     /translation="RNIAHKVRVKDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKK
                     NYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESL
                     ERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          171 a           83 c           70 g          127 t
ORIGIN      
        1 ctcgtaacat cgctcataaa gtacgtgtca aagaccgaaa agaaatctgt gatgacttta
       61 aggctgttta tcaagctaac tcaaaagaag aagcgaatac cttcttatcc ggcatgattg
      121 agaaatggaa gaaaaactat cctaaagtga cgcagtcact catagaaaac caagacttat
      181 taacttttta tgattttcca cctagcattc gtagaaccat ttactcaacc aatctaatcg
      241 agtctttcaa taagcaaatt aaaagataca gccgtagaaa agagcagttt caaaatgaag
      301 aatcactaga acgctttcta gtcagcattt ttgatacata caatcaaaaa tttctaaaca
      361 gaagccataa aggttttcaa caggtaaccg atacattagt ttcaatgttt actgagtaac
      421 taattatttt gcaggaggac aatttattta c
//
LOCUS       DBNICI010000335          451 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.348, whole genome shotgun sequence.
ACCESSION   DBNICI010000335 DBNICI010000000
VERSION     DBNICI010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.348"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..>451
                     /locus_tag="AC1ZCC_002928"
     CDS             <1..>451
                     /locus_tag="AC1ZCC_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HIQ1631117.1"
                     /translation="HENRKILFNGKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSG
                     SVWRWLKQFEKNGMLNIKSTTKYSVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNE
                     KNYKNEKNNKTSSSQPRKKRVYDTDSVYYILAEELFKQICQNQEIKKP"
BASE COUNT          189 a           82 c           90 g           90 t
ORIGIN      
        1 tcatgagaat cgcaagatac tttttaatgg aaaagagata caagtgaaca gcggagaatt
       61 cgtcacaggg cgcgatgcta tcacatctga gatgaacgaa ggcgtgaaac gtgaacatca
      121 agtgaacagc ggttctgtat ggagatggct aaaacaattt gaaaaaaacg gaatgttgaa
      181 catcaaatca actacgaaat acagcgttat atcaataaaa aactggtctt tgtaccaaag
      241 cagtgaacaa caaatgaaca tcaaacgcac aacaagtgaa caacaagtgc acacaaacaa
      301 gaatgaaaag aattataaga atgaaaagaa taataaaaca tcatcgtcgc aaccacgcaa
      361 aaagcgtgtt tacgacaccg actcagttta ctacattctc gcggaggagt tattcaaaca
      421 gatttgtcag aatcaggaaa tcaaaaagcc g
//
LOCUS       DBNICI010000336          449 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.349, whole genome shotgun sequence.
ACCESSION   DBNICI010000336 DBNICI010000000
VERSION     DBNICI010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.349"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            129..>449
                     /locus_tag="AC1ZCC_002929"
     CDS             129..>449
                     /locus_tag="AC1ZCC_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1631118.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEE"
BASE COUNT          160 a           69 c          129 g           91 t
ORIGIN      
        1 attcgaagcc ttcggcgtga gattgacgat ctggaacgaa caaaggcagt gatggtcaat
       61 gaaacggcta gaaaggcaat cgatttgcac atagagaatt taagaaggga aatccgtaga
      121 ttggaggaat gagcgtggat aaggaagcgg cttggcgaaa attaatgttg ctgattcaag
      181 atgagaactg gcaagaagat gaagcagtgg ttgctgaagt tcagcgtcta ggaaagattg
      241 ctaacggacg tatacgaaaa aagccagaca aaagaaaaca gcgcaaaggg aaaatcgtcg
      301 ttgttttaca cgaaggcaaa attttgatgc aaggaacagc tagtgagctg tctgcagaaa
      361 ctggatatac gcgtgggact attcgaacgt acgcttggcg aaatcacacc gatcgaaaag
      421 ggcacgaata taagtatttg gaggaagaa
//
LOCUS       DBNICI010000337          438 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.denovo.350, whole genome shotgun sequence.
ACCESSION   DBNICI010000337 DBNICI010000000
VERSION     DBNICI010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.denovo.350"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            77..427
                     /locus_tag="AC1ZCC_002930"
     CDS             77..427
                     /locus_tag="AC1ZCC_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HIQ1631119.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          180 a           47 c           71 g          140 t
ORIGIN      
        1 attggggaca atcatggtca gaagggttaa aacgttgggg agataatctt tttggtagtt
       61 ttagtggagg acgataatga cttcaataaa cattgatcac acaaaaaaag tcatacatga
      121 attatacaat agtttaatga cacgacctaa tcagacgaaa gcattattag atattactga
      181 tgttttattt caggtatata aaaaaattga tacagcaaaa tatcctgaaa ttcttgtaaa
      241 taaattagtt aattatattt acatcgtggg ttttgataat aaaatacgct ttattgataa
      301 tgatgaaaaa ttattaatag aattagggga tatttctaaa aaagctggaa ttaatagtaa
      361 atacaaagca aactttacag ataaatcgca attttatagt tattcagaaa caatgcctca
      421 acgataaaat atagtata
//
LOCUS       DBNICI010000338        10885 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.guided.097, whole genome shotgun sequence.
ACCESSION   DBNICI010000338 DBNICI010000000
VERSION     DBNICI010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.guided.097"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            12..1133
                     /locus_tag="AC1ZCC_002931"
     CDS             12..1133
                     /locus_tag="AC1ZCC_002931"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HIQ1631120.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            1391..2893
                     /gene="eat(A)"
                     /locus_tag="AC1ZCC_002932"
     CDS             1391..2893
                     /gene="eat(A)"
                     /locus_tag="AC1ZCC_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HIQ1631121.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2960..3487
                     /locus_tag="AC1ZCC_002933"
     CDS             2960..3487
                     /locus_tag="AC1ZCC_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIQ1631122.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            3698..4759
                     /locus_tag="AC1ZCC_002934"
     CDS             3698..4759
                     /locus_tag="AC1ZCC_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HIQ1631123.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            4762..5658
                     /gene="murQ"
                     /locus_tag="AC1ZCC_002935"
     CDS             4762..5658
                     /gene="murQ"
                     /locus_tag="AC1ZCC_002935"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HIQ1631124.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            5672..7654
                     /locus_tag="AC1ZCC_002936"
     CDS             5672..7654
                     /locus_tag="AC1ZCC_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HIQ1631125.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(7719..8165)
                     /gene="nrdI"
                     /locus_tag="AC1ZCC_002937"
     CDS             complement(7719..8165)
                     /gene="nrdI"
                     /locus_tag="AC1ZCC_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HIQ1631126.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            8376..9800
                     /locus_tag="AC1ZCC_002938"
     CDS             8376..9800
                     /locus_tag="AC1ZCC_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HIQ1631127.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(9981..10754)
                     /locus_tag="AC1ZCC_002939"
     CDS             complement(9981..10754)
                     /locus_tag="AC1ZCC_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIQ1631128.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(10751..>10885)
                     /locus_tag="AC1ZCC_002940"
     CDS             complement(10751..>10885)
                     /locus_tag="AC1ZCC_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1631129.1"
                     /translation="ELLGTVIQLLSAQNIHVTDVQHLTNDLESSLLEILDSVKGEEEK
                     "
BASE COUNT         3514 a         1900 c         2409 g         3062 t
ORIGIN      
        1 gagggataca gatggatatt ttggcggcaa ttgattcatt taaaggaagt gcttcttcat
       61 ttgaactaaa tcaggcagtt ctaaatggct gggaagatcg taaaggaaga aaaatcaatg
      121 tgccgattgc agatggcgga gaaaatactg cagaagcaat ctattacgcg ctcaggggaa
      181 catggcgtac cattgatggg atggatctct tattccgtca gcgaaaaatc agctatttat
      241 tgacaacttg gcaaggaaaa aacaccgcag tcatcgaatc tgcacaagca ttggggttag
      301 atctacttga taaaccaacg gatcaaacta tccgacttgc ttccagttat gccttaggag
      361 aacttattaa agatgcactg cttcaagaag tagaacaaat tattgtcact ttaggaggaa
      421 gcgggtgtag cgatggtggt ctaggattac tccaatcctt gggtgcttct ttagaagggt
      481 acacagaagg aaatccttta ttatctacta aaaaacttca ttttgatcga agaaacgttc
      541 ttgtttctaa agtaaaattg attatcgctg cagatgttac caatccatat acaggtcaac
      601 aaggagctgc ttggcaattt ggaagacaaa aaggaggaat agatacgacg ctagctttct
      661 tagataaaca agcaggaaag attgctcagc aaataaaaga tttctgggag gtggatttag
      721 atgatattcc aggaagtgga gcagcaggcg gtattggtgg agcccttttt ttgttgggag
      781 cgaagatggt ttctggtttc gatttagtca gccaaatcac tggattggaa aaaaagatcg
      841 aacaagcaga tgtcatcatt acaggagaag ggcggatcga tcagcaaacg atccacggaa
      901 aagttccata cggtgtagca gtgcttgcaa aaaaatatcg aaaaccaata atcgctattt
      961 gcggaagtag agaccaagag ctagaaggac tttctgattt tctcccaatt gtcttaagta
     1021 ttcatttggg tccaataggt ttagaagaag caatggaaca caggcaaaca ttagaaaaaa
     1081 caaaaatctt aggacagaca cttagcagat ttttcaccat cctcccgaag tagttgacaa
     1141 aatcattttc tttatgctat cgtaatttca aatataggac aaagtgaggg atccgtgcat
     1201 tgaaaaatta agtcgattga tgaaagctgc attcagactg taccttgtgg agacaaggaa
     1261 gcagtgtgca gattttcggg attggcttgg ttttttcgat gtatgggtct ttttgtgtgt
     1321 atccaaccct tgcatcctct ttagccagtt ctgaaaatag gactggtctt tttttacgag
     1381 gtgatggaaa atgtctaaaa tcgaaataaa aaatctgaca ttcggctacg acagccaagg
     1441 cacattatta tttgaacaag caaatctaaa ttttgacaca caatggaaac taggacttat
     1501 cggacgaaac ggtcgaggaa agacaacttt actgaatatt ctacaaaaca aactacctta
     1561 tcaagggcaa gtaatccatc agcaagaatt tgcctatttc ccgcaacaga caaaagataa
     1621 agaacgttta acctattacg tgttaaatga tattacggat tttgagatat gggaaatcga
     1681 aagagagctc caattgatgc aaacagatcc tgaaatctta tggagagaat tcagcacact
     1741 atcgggggga gagaagacaa aagtcctact ggcactttta tttgtggatg acactcattt
     1801 cccgttaatc gatgaaccaa cgaatcattt ggatatctct ggtagaaaac aagtagcggc
     1861 ttatttgaaa aagaaaaaac aaggcttcat cgtggtcagc catgaccggg gatttatcga
     1921 tgaagtagtg gaccatgttt tagcaatcga aaaaagtcaa ctggaacttt atcaagggaa
     1981 tttctctatc tatgaagaac agaaaaaact tcgtgatgaa tttgaaatgg ctcaaaatga
     2041 aaaattgaaa aaagaagtca gtaggctaaa gaaaacagca gctgaaaaag ccgaatggtc
     2101 tcgttcccga gaaggagata aaacaaagaa acaagtcgga ttcatcgata ctgaatctag
     2161 acgagtgaat aaaggagcag tgggtgctga tgctgcacgg acgatgaaac gatccaaagc
     2221 aatcgtgaat cggatggaga cccagatcag cgagaaagaa aaactattaa aagatatcga
     2281 atatatcgat tctttgacga tgaatagcca agcgtctcac cataagcgac ttttaagcgt
     2341 agaagatctt caattagggt atgaaaatct gttattcgag ccaattcatt ttacaatcga
     2401 gcctcatcag cgggtggcga tttcaggtcc taacggtgca ggaaagtcat ccattatcca
     2461 ttatcttctg ggggcattca acggcaaggt tataggagaa aaataccagc caaaacatct
     2521 gagcattagt tatgcaagcc aaaattatga agacaatcga ggaacgttgg cggaatttgc
     2581 agagaaaaac caagtagact accaagcatt tttgaacaac ctccgaaagc ttgggatgga
     2641 aagagatgtt tttcataaca agatcgagca gatgagtatg ggccaacgga aaaaagtgga
     2701 attggctaaa tctttatcac agccagctga actatataca tgggatgaac cattgaatta
     2761 tttggatgtc ttcaatcaag aacaattaga acaactgatc ttgaacgtga aacctgccat
     2821 gttactagtg gaacatgatc aaaccttcct ggataaagta tctactgaga ttatttctct
     2881 tgagagaatc taacccttgt aatctcaaga acaacaaggt atggttaagg ggaactttgc
     2941 caacgaagga gagaatcaca tgctttatgt aacgagacac ggagaaacaa cttggaacgc
     3001 acaaggatta gtttgcggac gggcagatgt gccgctgact gaaaaaggac agatgcaggc
     3061 acagaaacta gcagaaaagg tagtagattt gcctgttcca atcacgaaaa tcatccattc
     3121 ccctcttcaa cgagcaagag atacagcaca agcagttgct gatcgtctca gcttgccgct
     3181 gaccgttgat gaacgattgg tagaaatgga ttttggggat tatgatggac tgccaagcaa
     3241 agatgagaac tttcaaaaag cacgtttagc gttcgctgta cgattcccaa atggggagtc
     3301 agtcttagat gtgtatgcac ggatcgttcc tttattgaaa gaatgcatcg aagacgaaga
     3361 aaatgtctat ctgttagttt gccataatgc cttgataaga gtgatcaatg cttattttca
     3421 tccgatgcct aatgaggggt tctttacatt catggtagat aatacagaat tggtttctta
     3481 tgagtaatta tgtttttgtg ttatttaaat aaaatatttc aaggcactgc ttttgcttag
     3541 cagtgccttt tttaggttat gacaattctt ttgataatat aaactcaacc tttagaaatg
     3601 aaatttaatt attttaataa aagtattgaa attttgtttc tttagatata tactctagtc
     3661 atagaaatga agacggatac agaaaaagag ggataatatg tttggctttt caatatttat
     3721 gaacgattct ttgtcagaag agaagaaaca gtatatccgc caaatggcat ccaatggctt
     3781 tgtcggtatt ttcacttcca tgcatatacc agaagatgat gtttcggcat atcaaaagcg
     3841 attgatcgaa ctaggaagcg aagcgaaaaa gaataaacta gaattgatgg tcgatatttc
     3901 aggagaggca ttggaacgag cgggtttttc attagaagat ctaacgcctt tgaaagcaat
     3961 tggtgtaaca ggtttaagaa tggattatca tatttccaat cagcagattg ccgactggtc
     4021 acatcaattg aaaatcagtt tgaatgctag cacgatcacg cctaaagata tcgatgaatt
     4081 gaaagaagca gaagctgatt tctcacagat tgaagcttgg cataattatt atccacgtcc
     4141 cgaaacaggg ctggacaaag aatggtatca gaaaaaaaat caatggctca aaattcaagg
     4201 tctccttgtc caaggttttg taccaggaga tacagagttg agaggcccac tatatcaagg
     4261 attgccaact ttggaagaac atcgagggat ccatcctctt gcaggcgctc tagatctatt
     4321 agcaagtaac acggatattg tttacatcgg agatgcaggt ctgtcagaaa acgttcagga
     4381 gcaatttgct tctttccaaa aagaacagac agtcttgctc catacggaac ctgtagatga
     4441 agaattttac gaatatatat tagggaagca tacgaatcgg caagacgatg caagagatgt
     4501 catacgaagc gtagatgcac gttttcgaga gattccgcct atcccagcaa gaaatacagc
     4561 tacgcgtatg aaagggtcta ttactttaga taacgaaaag tatttgcgct atatgggtga
     4621 gatccaatta accaaatatg atttgccagc ggatgaaaaa gtgaatgtag tagctaaagt
     4681 tatcaaagaa gagctaccat tgatcaacca aatcaaagct ggtatgaact atcaatttat
     4741 aagaaaagaa ggaagataaa aatgagcaaa atcaatttag agaacctaac aacggaacgc
     4801 agaaaccaag aaacttttgg attagatgag atgagtattt ctcaagccgt tcaactaatg
     4861 aacaaagaag atcagaaagt ggccgctgcg gttgaaaaag aactgacaag tatcgaacca
     4921 gtgattgcta acacgattga gtcctttaaa aaaggtggac gtctgattta tatgggagcc
     4981 ggaacaagtg gaagactagg tgtgttagat gctgctgaat gcgtaccgac attcggtgta
     5041 gaaccggaaa tggtcatagg tctaattgct ggtggagaag aagcaatgac tgtagctgtc
     5101 gaaggggccg aagattctta tgagctaggg caagaagatc taatcaagct gaatttgaca
     5161 aaaaatgata tagttattgg tatcgcagct agcggtcgta caccatatgt gatcggtgga
     5221 ttagattacg caaaatcgat cggtgctgtc acaggaacga tttcttgcaa taaacaagcc
     5281 gaaatcagta aacacgcgga tttcccaatc gaagtcgatg caggtccaga atttttgaca
     5341 ggctctactc gattgaagtc aggaacagca caaaaactta tcttgaatat gatttctact
     5401 atcagtatga ttggtatagg taaagtattc aataacttga tggtcgatgt caaaccaaca
     5461 aatgaaaaac taatcgaacg ctcaaaacga atcatcatgc aagcgacaga agctgattac
     5521 gaaacagctg aaaagtactt tgaagaagca gatcaaaatg ttaagctggc tatcgtcatg
     5581 attttgacaa atagtgataa agaggaagct caagcaaaat tagctgaagc aaacggattt
     5641 gtaaaaggga ccttatgagg gggaaaagac catggcaaaa aacgaactga ctttggaaga
     5701 actggcaaaa caaatttata aaggggcagg cggcatgggc aatgtagaaa gcattgtcca
     5761 ctgcatgact cgtgtgcgta tgaagatcat caatgatgat cttgtaaatg tctctgaatt
     5821 aaaagcgatt ccaggagtgc ttggtgtagt agaagatgaa cagcttcaag tcatcatcgg
     5881 acctgggaaa gtgaacaaag tagcacagca aatggttgat ttggcaggcg tgaaattagg
     5941 agaaaaactt tcttctgtaa gtacagctac agcaaattca tcagcatctg gaaaagaccg
     6001 tgtaaacgaa cgggcacaag aaatgaaagc ggcacaaaaa tcgaaacaaa aaccatctaa
     6061 aatcaaatcg gtattaaaag acatttctaa tatctttgtg ccaatgattc ctgcatttgt
     6121 cggtactggg atcgtggcag ggattgccgc agttttagca aacttagtca cagccggaac
     6181 acttgatgca gcaacatggc aacaatatat cgatgtgatg aacatcttga aaaacgggat
     6241 gtttagctac ctagttatct atacaggtat caatgctgca caagtttttg gtgcaacacc
     6301 aactcttggt ggagttatcg gggccgttgt gatgctgaca gggatgaatc cggaagcacc
     6361 actgaaaaac ttatttactg gtgaagctct tgcagctgga caaggtggga tcctaggtgt
     6421 catcttcgct gtttggctat tgtcaatcgt tgagaaaaaa ctgcataaag tagtacctga
     6481 tgcggtagat atcatcgtta caccaactct ttcattgatt gccatcggtc tgattgaaat
     6541 tttcttcatc atgcctttag caggtttcat ttctgatggc atggtcggtg ggatcaactg
     6601 ggtacttggc gttggtggag catttgccgg cttcgttcta ggcacattat tcttgccgat
     6661 ggttatgttc ggactgcatc aaattttgac tccaatccat gtgcaaatga ttgatgaaac
     6721 tggtcgtacg ctattgcttc caattcttgc gatggctggt gcaggacaag tgggtgcagc
     6781 attggcatta tggattcgct gcaaaaaaga taaagaatta acagaaatga tcaaaggagc
     6841 tttaccagtc gggatcctag gaatcggcga accattgatt tacggagtaa cattacctct
     6901 aggacgccca tttattactg cttgtatcgg tggaggtatc ggcggggcag tgatcggaat
     6961 gatcggaaat gtcggggcta tcgctattgg accttcagga gccgctttga tcccattgat
     7021 ctctgacggt aaatggttag gctacatcct tggattattg gcagcttatg ctggtggctt
     7081 cgttgctaca ttcttcttcg gtattcctaa agaccgttta gaaaaagaag aattagcgac
     7141 tgaaacagcc acagtaaatg aagtgcctga aaaagtagta cctcaaactt cagctgaaag
     7201 tgtgactatc ctctcatcag ttgctgacgg aacagttgtt tctttagata aagcaagtga
     7261 tccagttttt gcacaaaaga tgatgggtga aggctacttt gtagaaccag aaaacggaca
     7321 tatctattct ccagttactg gggtagtcag ctctgtcttc ccaacgaaac acgctattgg
     7381 gatcacaacg ccaaatggtt tagaaatctt gcttcatatg ggaatcaaca cagttgactt
     7441 aggcggtacg ccatttgatg tgaaagtaac agaagggcag caagtgacac ctgatacgct
     7501 tgtagcagaa gctgacttag aagcgatcaa agcagcaggc aaagaaacat ccatgatcgt
     7561 cttggtaacc aatatggatc gtgtaaaaaa ctttgtcttg gaaaaaacag gtaaagcaaa
     7621 agcaaaagca cctgtgatgg atgtcaaagc ttaatatgat agaaacaata gaaacgaaag
     7681 aagaaactga aatctatttt tcagcttctt cttttttatt atttattgtt ttctgctaaa
     7741 atctgataaa ttcgtgtcac atcagatggt gttccccgta attcataatc tgccaagaaa
     7801 ggaaagccaa atgtttgtgc atattgtttg gctgttagac aatactgatt attgaagttt
     7861 ttatttccgc taccgactac accaagacaa agtttatgat tgtcttcatg ttccagatat
     7921 tcacgcatcg tttcggtcaa gatttctgta tcaccgttat ctaaaccatt tccgccgtct
     7981 aaataagtag gaacgaaagt aaaaaatggt tctgtttctt tcgtaaaatc actattttca
     8041 tggatttctt ttaaagtaac ttcaggattg acaggatctt ctgcatgcat tttttcagca
     8101 tattctgcta gatgtttggc aaaggcacgc gtatttcctg aaatcgatat atataaaatg
     8161 ttcatggatc atcctccaat tctttccttt tagtatagca tcttttttaa gttttttggt
     8221 ggtgtaattt ggaaagggac taggcgcttt gcttgtataa tgagaaaaaa gccggtagaa
     8281 ttatatttct attaaagttt ttattaatta agtaaaaata aaaaaggttt gttatacttt
     8341 ttgataaaag cggttttatt ttggaggatg cttacatgga tacagagcaa cagaaaaaag
     8401 gaattggttt aattccactt gctgccttag taattggttc agcgattgga ggtggcgtat
     8461 ttggtattat gacggacatc tccggatcgg ctggcggccc agctattttc agttgggtac
     8521 tcgtaggaac agcaatgttg atgctctcct tgtcgatcaa taatatcaat aaaaaacgtc
     8581 cagatttaaa aggcgggatc tttagttacg cagaagaagg ttttggccgt ttcgctggtt
     8641 ttatcagcgg ttggggttac tggctcacat gctggttagg caacgtagct tttgcaacat
     8701 tgttgatgag cgcattagga tatttcttcc cagtatttga aggtgggcaa aatctggcat
     8761 cgatcgtgat gagtacagtc tttttatggg gatatgcatg gctagtgaat cggggagtcg
     8821 aaaatgctag tttcgtcaat gctatcgtca cattctgtaa attagtgcca ttatttttat
     8881 ttattgtagt agctatcagc acgttttcga tccaacaatt cattgataac ttttctagcg
     8941 acattatatt gaatgcgaat ggacagccga tcccgttttc cagtcaagta ttgagttgca
     9001 tcatggtgat gctttgggta tttgttggta ttgaaggagc ctctgtcgta ggttcacgag
     9061 caaaaaaaca atcagatgtt gggaaagcaa cgattctagg gatattcggc ttgattatta
     9121 tttacgtctt ggtttccatg cttccatatg ggacaatgac gatgaatcag ctgcaaacga
     9181 tcaaggcgca gcctgctatg ggctatgtgt ttgaaatgat ggttggtaaa tggggagcag
     9241 tcgtgatcaa cggcggtttg atcatttctt tagttggtgt ttggctctca tggacgatct
     9301 taccgattga aacaatggga aacatggctg aagacggact tttgcctaaa gcatggggaa
     9361 aactcaacaa aaatggggca ccaacttatg ctatcgtatt gacgacgtta tgtacgaatg
     9421 tctttttact ttcattatta ttcactgaca gtgcttataa ctttgcttac acacttggta
     9481 cagcagctat tttcttcacg tggcttttct taggattgta ccaaatgaaa ctttcttatc
     9541 agcggaaaga atgggtccaa tttttagtag ggcttttagc aagtgccttt caagtatggg
     9601 ctatgctagt cgtagccttt aatgaagtaa tgcttgttct tgttcttttc ttgccaggca
     9661 tttattttta cctcaaagca cgtaaggagc aaaacgagcc tgtacaacat atccacttag
     9721 ataagatttt acttataggt gtgacgatgg tcggagtgat tgcaatggtt ttatttgcca
     9781 ctgggatgct tcacgtatag aagtataaaa gaagtataaa gagaggttgt gagcctgtcg
     9841 tttattcaaa aacatactag gaaaggagct gtgacacaaa tgttgtcaca gctccttttt
     9901 cagaataaac ggtgtttaag agacagccaa tcggcaataa ggctgaacgt ctctttcaca
     9961 gcctcttttt tgatgagtca tcatacattt tttcgcttaa atacgagata tcctaatgct
    10021 aagaacaaaa cgatgtaagc taaattaccg aatagcattt gattagtaga aagatgagtc
    10081 aattgcttca tgatctcttc gtttccaatt tggttttgca gattcaacat actgatcgga
    10141 ttccatttga tccactccca tttttggatg agtgcaataa ggataccaga tatgattgac
    10201 gaagcaaaat aaaagacgat tcctgctgag atggatgcgc cggaactatt gataaagcaa
    10261 gcaatcatca acactaaact taaaatcagc cataaaccga tataactacc caaagtataa
    10321 gtgaacaagc tttggatcag tgtttgacca gtagaaacct tctccgtgaa ggaaatcgaa
    10381 gggaaaagaa tcagtttcat caacacagtg acaataatag ctaaaaggta taaataaaca
    10441 gaataaataa ctaatgtgat ccatttgctt actaatattt ctcctcgaga atactttcga
    10501 taaaggagat ttttcaatgt accatattgt gcttccatcg aaatgatcgt gcttgcagct
    10561 gcaatcatga taaagacaat ccaagatgtg gcactaaaaa gctgagcagt catttcaact
    10621 ggatcaatga tcgtcgtgta ttttttggct agaagtgctg ttcctatgag caaaacaact
    10681 aatagaacag ataaaacagc agttgacttt ttcttgatca atttaaataa ttcttgtttt
    10741 acaagcaatg tcatttttct tcttccccct ttactgaatc cagaatttcc aatagagaag
    10801 attccaaatc attcgtcaga tgctggacgt ctgtcacatg gatattttgt gcagacaata
    10861 gctggatcac ggttcctaat aattc
//
LOCUS       DBNICI010000339         6156 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.guided.148, whole genome shotgun sequence.
ACCESSION   DBNICI010000339 DBNICI010000000
VERSION     DBNICI010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.guided.148"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..1506
                     /locus_tag="AC1ZCC_002941"
     CDS             <1..1506
                     /locus_tag="AC1ZCC_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIQ1631130.1"
                     /translation="AKEKIKKLRVAAYCRVSTETEEQNSSYEVQVSHYTEFIKKNNEW
                     EFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRFARNTLDCLQYIRQ
                     LKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVKLGLQYRYQQGKVQ
                     VNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGRALEKDGILTAAGK
                     PRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQYYVENSHEAIIPK
                     ELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDIYRRVYWNIHGRKE
                     FVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMIKTLEENIHAVIGE
                     TTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQILLVEDASLSGEN
                     ERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKSGIEMEI"
     gene            1649..1873
                     /locus_tag="AC1ZCC_002942"
     CDS             1649..1873
                     /locus_tag="AC1ZCC_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1631131.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            1888..2757
                     /locus_tag="AC1ZCC_002943"
     CDS             1888..2757
                     /locus_tag="AC1ZCC_002943"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIQ1631132.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            2738..3472
                     /locus_tag="AC1ZCC_002944"
     CDS             2738..3472
                     /locus_tag="AC1ZCC_002944"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIQ1631133.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            3505..4413
                     /locus_tag="AC1ZCC_002945"
     CDS             3505..4413
                     /locus_tag="AC1ZCC_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HIQ1631134.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            4410..4952
                     /gene="sat4"
                     /locus_tag="AC1ZCC_002946"
     CDS             4410..4952
                     /gene="sat4"
                     /locus_tag="AC1ZCC_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HIQ1631135.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5045..5839
                     /locus_tag="AC1ZCC_002947"
                     /note="aph(3')-IIIa"
     CDS             5045..5839
                     /locus_tag="AC1ZCC_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HIQ1631136.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT         2115 a          964 c         1403 g         1674 t
ORIGIN      
        1 gcaaaagaaa agataaagaa acttcgtgtt gctgcctatt gccgtgtttc tacagaaaca
       61 gaagaacaaa attctagcta tgaggttcag gtctcacact atacagagtt tataaagaaa
      121 aataatgaat gggagtttgc tggcatcttt gcagatgatg gtatctctgg tacgaacact
      181 aaaaagcgtg acgaatttaa tcgcatgatt gcagagtgta tggacggtaa catcgacatg
      241 gttattacaa aatccatcag ccgatttgcg cgtaacaccc tagactgcct tcaatacatt
      301 agacagctca aggataagaa catatcagtt tattttgaga aagagaacat caacaccacg
      361 gatgccaaag gtgaggtttt gctgactatt atggcatctt tggcacagca ggaaagccag
      421 agcctttcac aaaacgttaa gcttgggcta cagtaccgat accaacaagg aaaggtgcag
      481 gtcaaccata agcgatttat gggctacacg aaagatgaag atggaaattt aactattgtc
      541 cccgaagaag ctgagattat caaacgcatc taccgagaat accttgaggg tcagagtcta
      601 gtgggcattg gtcgagccct tgaaaaggat ggtattttaa cagcagcggg aaaaccaaga
      661 tggcgaccag aatcagttaa gaagatactt caaaacgaaa aatacatcgg agatgccctt
      721 ctgcaaaaga ctgtcacagt agattttctg accaagaaac gagtgaaaaa tgaagggcat
      781 gttccccaat attatgttga aaatagccat gaagcgatta ttcctaaaga gttatttttg
      841 caggcgcaag aagaaattca tcggagaagc aatatctaca caggagaagg caagaacaaa
      901 agaatttaca gtagcaagta cgctttaagt gccatcacct tctgtggaga ttgtggcgat
      961 atttatagac gggtctattg gaatatccac ggaagaaaag aatttgtctg gcgatgcgtg
     1021 actagaatcg agcaaggtcc tgaagtttgt aagaaccgaa ccgtaaaaga agatgaactt
     1081 tatggtgcag taatgactgc gattaataag ctacttgcag gtgggaacaa tatgataaag
     1141 acactggaag agaatattca tgctgtgatt ggcgaaacca cagaatacca aatttcagag
     1201 attaacaact cactagagga aaaacaaaaa gaactaatca agctggcgaa caagggtcaa
     1261 gactatgacc acttagcaga tgagattgat gagctgagag acaagcgaca gatcctttta
     1321 gtagaagatg cctccctcag tggcgagaac gagcgaatca atgagctgat tgaatttatc
     1381 cgcaagaaca aattccgcac cttagaatat gatgataagc ttgtaaggaa gataatccag
     1441 agcgttacag tctatgaagg ccacttcgtc atagccttca aatctggcat cgaaatggaa
     1501 atatgaaaac cagaagtaaa tagcccatga ctctacagta gagttgtggg ttttcttttg
     1561 cttgctgatt ataataatat tttaataact attgtgttta tcaaccaaat ggtttataat
     1621 attcttagcg taaacttgga ggtgctgtat gactacggca gaaatgatta aagaactgtg
     1681 tgagcaaatg aatataagtg tttccgaact tgctagacgt attggccaga ctccacagaa
     1741 tttcaataaa aaattacaac gagaaacagt aaccttggat gagttgaagg ccattgctga
     1801 tgtgctaggt gtcaagtttg tgcaggcatt tattttacca gatggagaag aaataaaaat
     1861 atctaacgaa taaaggagga tgtttatatg gtaggtaatg ttattcaaat agtaacagag
     1921 aaattatcat ctttgccttt tatagaaggc atcgtattag ggggctcacg tgcaagaggc
     1981 acccatacag aaaattctga tatagatatc ggaatctatt acaattcaga ctcatttgac
     2041 ctgacagcga ttaatcaaat tgctacagag ttggatgatg agaatagaaa caaccttgtt
     2101 gtacctcccg gagcgtgggg tgattgggtt aatggcggtg gatggttagt tataaacggg
     2161 tgtcatgttg acttgatatt acgtgatata aaacgggtgg aacaaataat caaagatacg
     2221 gagcaaggaa ttgttactgc caattatcag accgggcatc cccatggtta tataagtgct
     2281 atgtatcgtg gagaattagc gattagtaaa atactatatg ctaagaacga aagcttatgc
     2341 gaattaaaaa agcaggcaga aatttaccct actgctctaa agaaaagctt gatgaacttt
     2401 tttatatttg aagctgaatt ctctttaatg tttgtaaaag caaatgcggg ggcagaggat
     2461 aaatattata ttgcaggcca tgtttttcgt attatttcat gcttaaatca agtactattt
     2521 gcatgtaata atgcttattg cattaacgaa aagaaagcta taaaactgct tgaaactttt
     2581 gaatataaac ctgaaaaata tgccgaaaga gttaatcata tttttgaagt actcggtctt
     2641 tctctttttg aatgctacga catgaccgag aaactttata aagaagtgaa tgaaattgta
     2701 tcggagataa ataacttttt aaacgaggag agttcagatg aaagaaaaca aatatgatga
     2761 taatatattt tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac tggctggtgc
     2821 gggagaatgg gagactttga aaaagatgct acctgatttt aagggtaagc gtgtgcttga
     2881 tttaggatgc ggctatggat ggcactgtat atatgcgatg gaaaacggtg cttcctctgt
     2941 agtaggtgtt gatatttctc ataaaatgct cgaagtagca aaaggaaaaa cccattttcc
     3001 acagattgaa tatgaatgct gtgccataga agatgtggat ttcccagagg agagctttga
     3061 tgtaatacta agttcgcttg cgtttcatta tgtagcagac tatgagaatt taataaaaaa
     3121 gatatatagg atgctgaagg ctggtggcaa tttagttttt acagttgaac atcctgtttt
     3181 tactgctcat ggaacacaag actggtatta taacgaaaaa ggagaaatac tgcatttccc
     3241 ggtggacaat tattattatg agggcaaacg gacagctatg tttttggaag aaaaggttac
     3301 aaaatatcat agaacactga ccacatatct aaatacactg ctttcaaata gttttataat
     3361 aaatcagatt gtggagccac agccgccaga gaacatgatg gatattccgg ggatggcgga
     3421 tgaaatgcga cgcccaatga tgctgattgt atcggcaaaa aagaagatgt aataatatag
     3481 aaaaaataaa cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga tttagtactt
     3541 tctttagcag aacaggatga acgtattcga attgtgaccc ttgaggggtc acgcgcaaat
     3601 attaatatac ctaaagatga atttcaggat tatgatatta catattttgt aagtgatata
     3661 gaaccgttta tatctaatga tgactggctt aatcaatttg ggaatataat aatgatgcaa
     3721 aagccggagg atatggaatt attcccacct gaagaaaagg gattttccta tcttatgcta
     3781 tttgatgatt acaataaaat tgatcttacc ttattgccct tggaagagtt agataattac
     3841 ctaaagggcg ataaattaat aaaggttcta attgataaag attgtagaat taaaagggac
     3901 atagttccga ctgatataga ttatcatgta agaaagccaa gcgcaaggga gtatgatgat
     3961 tgctgcaatg aattttggaa tgtaacacct tatgttatta aaggattgtg ccgtaaggaa
     4021 attttatttg ctattgatca ttttaatcag attgttcgcc atgagctgct gagaatgata
     4081 tcatggaagg tcggcatcga aacaggcttt aaattaagtg taggcaagaa ctataagttt
     4141 attgaaaggt atatatccga ggatttgtgg gagaaacttt tgtccaccta ccggatggat
     4201 tcctatgaaa acatatggga agcattattt ctatgccatc aattgttcag ggcggtatcc
     4261 ggtgaggtgg cggaaaggct tcattatgcc tatccggagt atgataggaa tataacaaaa
     4321 tataccaggg acatgtataa aaaatacact ggtaaaaccg gctgcctgga tagcacatat
     4381 gccgctgata tagaagagag gcgggaacag tgattacaga aatgaaagca gggcacctga
     4441 aagatatcga taaacccagc gaaccatttg aggtgatagg taagattata ccgaggtatg
     4501 aaaacgagaa ttggaccttt acagaattac tctatgaagc gccatattta aaaagctacc
     4561 aagacgaaga ggatgaagag gatgaggagg cagattgcct tgaatatatt gacaatactg
     4621 ataagataat atatctttac taccaagacg ataaatgcgt cggaaaagtt aaactgcgaa
     4681 aaaattggaa ccggtacgct tatatagaag atatcgccgt atgtaaggat ttcagggggc
     4741 aaggcatagg cagcgcgctt atcaatatat ctatagaatg ggcaaagcat aaaaacttgc
     4801 atggactaat gcttgaaacc caggacaata accttatagc ttgtaaattc tatcataatt
     4861 gtggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg
     4921 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg
     4981 tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata
     5041 ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg
     5101 cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga
     5161 aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg
     5221 ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt
     5281 tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc
     5341 ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat
     5401 caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg
     5461 cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg
     5521 ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa
     5581 gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa
     5641 agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta
     5701 tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga
     5761 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt
     5821 actggatgaa ttgttttagt acctagattt agatgtctaa aaagctttaa ctacaagctt
     5881 tttagacatc taatcttttc tgaagtacat ccgcaactgt ccatactctg atgttttata
     5941 tcttttctaa aagttcgcta gatagagttc tatattagag atgtaaagag ttttggtgaa
     6001 gaggtttgtg agtatggctt ttataaccag ggtgcaatga gaagcactaa cagcagctag
     6061 cttaagaacg cataggatat actttttatg gaagtcacag aggaggaata aaatttgaac
     6121 aggattaaca gagtaacctc tattcttatt cagctg
//
LOCUS       DBNICI010000340         5895 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.guided.156, whole genome shotgun sequence.
ACCESSION   DBNICI010000340 DBNICI010000000
VERSION     DBNICI010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.guided.156"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            422..886
                     /locus_tag="AC1ZCC_002948"
     CDS             422..886
                     /locus_tag="AC1ZCC_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIQ1631137.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            870..1499
                     /locus_tag="AC1ZCC_002949"
     CDS             870..1499
                     /locus_tag="AC1ZCC_002949"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIQ1631138.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1853..3331
                     /gene="msr(C)"
                     /locus_tag="AC1ZCC_002950"
     CDS             1853..3331
                     /gene="msr(C)"
                     /locus_tag="AC1ZCC_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HIQ1631139.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3378..3824
                     /locus_tag="AC1ZCC_002951"
     CDS             3378..3824
                     /locus_tag="AC1ZCC_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HIQ1631140.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(4023..4538)
                     /locus_tag="AC1ZCC_002952"
     CDS             complement(4023..4538)
                     /locus_tag="AC1ZCC_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIQ1631141.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4655..5857
                     /locus_tag="AC1ZCC_002953"
     CDS             4655..5857
                     /locus_tag="AC1ZCC_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIQ1631142.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1819 a          981 c         1245 g         1850 t
ORIGIN      
        1 atgttttttg tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc
       61 aaaaaaggtt gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc
      121 caatttctaa tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt
      181 aaaattatct actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt
      241 tttagcgttg tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc
      301 tatacttttc tattgagagc gtttttttta ttacacagct gattctttga cttttttctt
      361 tttttattct attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat
      421 tatgtcttta gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat
      481 cgtccagatc tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag
      541 actgaaagaa gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca
      601 aggagcagtt actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag
      661 aaatcctcag aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat
      721 caagcattgt gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga
      781 tgtagaaatg aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat
      841 aacaacagaa acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta
      901 tgactggtag aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa
      961 tgctggaaat gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc
     1021 gctttgtggt cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca
     1081 atacgagaca aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt
     1141 acgaatatgg cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag
     1201 tcagagatca acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa
     1261 tgaacgatgt agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc
     1321 gtgatcatgg ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg
     1381 catttggttt ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg
     1441 tagaagaagg ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa
     1501 aggttttcag ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat
     1561 aaaaaaggag gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa
     1621 caactaaata aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat
     1681 tttttcgaac gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt
     1741 tatttcgttc agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct
     1801 cttttttagt atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa
     1861 tttagcagta aatataacaa atctacaagt cagctttggc aaccagctag aactatctat
     1921 tgattctctt cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg
     1981 taaatcgact ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca
     2041 aacagagatc accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct
     2101 aaatttggaa ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg
     2161 gagcggaggg gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg
     2221 gatgcttcta gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga
     2281 agagcttcgt tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga
     2341 tgagctagca tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa
     2401 ttatagtgcc tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga
     2461 gtcgatcatg aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc
     2521 ggaaaagttg gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt
     2581 gtcttcctct aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt
     2641 agagagaaga ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg
     2701 ttttccagta ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt
     2761 ccaattggaa agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg
     2821 taaaaaaatc gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat
     2881 ccgcaaacaa ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca
     2941 aaagggttat caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga
     3001 gttttcagaa tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt
     3061 gacgaaaccg ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct
     3121 ttttactaag ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc
     3181 aacgatcgaa gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc
     3241 tcatgattct tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa
     3301 aataaaaaaa gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt
     3361 taaaggagtg gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga
     3421 tgtgtttttt gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc
     3481 atttatcaag tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg
     3541 tcttcatcat cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa
     3601 gcaaggtcca aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta
     3661 tgtctttatt cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata
     3721 tgaatgcttg gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc
     3781 cgctttcaag aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt
     3841 gaaaaaaggc cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag
     3901 tgatccgaac gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt
     3961 gctcttttat tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc
     4021 ccttactcaa aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc
     4081 tgacaaagca taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct
     4141 agcgagaggt cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct
     4201 ctagtgaata gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt
     4261 gctagaaatt catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca
     4321 agagcacgtc cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc
     4381 aaaacggtca cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga
     4441 tgggcttccc aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat
     4501 aaaagcacgc ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta
     4561 ttttctttat tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa
     4621 ataaatcatg tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga
     4681 tatacaagaa caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc
     4741 tacctgtatg atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc
     4801 tgcacagtta aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt
     4861 cactggtgtg gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac
     4921 attgatgaca ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa
     4981 tatcggaatg ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc
     5041 tactgcttta gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat
     5101 gtatgaagca gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg
     5161 cagtggttca tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat
     5221 agccctagcg gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga
     5281 aggtgtcaaa gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata
     5341 taatttcgga ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga
     5401 attagaagtc ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt
     5461 cgttgcaccg gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt
     5521 cttattcatt atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc
     5581 gatcagcatt gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat
     5641 tgctatggaa ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg
     5701 ctttttcggt ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga
     5761 aagctatgcg ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta
     5821 tcaccgtaat tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc
     5881 tgtcctgcat caagt
//
LOCUS       DBNICI010000341         5743 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.guided.161, whole genome shotgun sequence.
ACCESSION   DBNICI010000341 DBNICI010000000
VERSION     DBNICI010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.guided.161"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(39..233)
                     /locus_tag="AC1ZCC_002954"
                     /pseudo
     CDS             complement(39..233)
                     /locus_tag="AC1ZCC_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(767..2926)
                     /locus_tag="AC1ZCC_002955"
     CDS             complement(767..2926)
                     /locus_tag="AC1ZCC_002955"
                     /inference="COORDINATES: protein motif:HMM:NF033527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HIQ1631143.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSGK"
     gene            3072..3647
                     /locus_tag="AC1ZCC_002956"
     CDS             3072..3647
                     /locus_tag="AC1ZCC_002956"
                     /inference="COORDINATES: protein motif:HMM:NF012464.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIQ1631144.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3861..4556
                     /gene="vanR-A"
                     /locus_tag="AC1ZCC_002957"
     CDS             3861..4556
                     /gene="vanR-A"
                     /locus_tag="AC1ZCC_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HIQ1631145.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4534..5688
                     /locus_tag="AC1ZCC_002958"
     CDS             4534..5688
                     /locus_tag="AC1ZCC_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HIQ1631146.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1796 a         1104 c         1087 g         1756 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccct gttcaaaatc taaaatcaac
       61 acatcatggg gagtgtgata ataaatattt tcccttaatg taccaacaat ggtaattcgg
      121 tcaattgacc attgcatttt taagggggtg cacatagttt gtgcatttaa ccgcctgtta
      181 gaaatacggt taagtttttc ccactcattt acgattatgc cttttttact caaaaatagc
      241 tcctcctcaa cgaaaaaaga gattcttgga aagaatttcc agagttcacg taactctaaa
      301 aatccacatc caaaaatctc tagcttgaag aaacctaaaa atattcgtat aatgaacttg
      361 agttacggaa ctaggtgaag tagtttccgt aaaggtcgag agtgcgtcaa cactgctcga
      421 ctattttttt actctttttt ctcattttct ttgattatag cactcctcta gaaaaaataa
      481 aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc gaaatagaat
      541 ggcgctgcca gcatttattt cgttcagcta tcggctacac aatctaacac gaccgttccg
      601 ctaccagtcg ccttacaggc tccattttcg ctcctctaac gtgtttcacc gattgtgccg
      661 ataactaaac gaaataaacg ctaaaaccgc tcagaacgca aaatatgcca ttttagagct
      721 attgtagaaa atatagtttg aaatgcaatt ttcttctttc agctttttat ttcccacttg
      781 taaatcggta gatggtggct ccttttccag ttccataatg tggatttgca tctgcatgta
      841 gtgatgaaac acctagctgc attctcatac catctgacga agatgttgta ccgtcgcccc
      901 aatagaaagg caattgtaat ttatgatgaa agtttactaa tatggcttgg gctttattca
      961 tggcatcttc atacatgcgc cattgagata cattggctag ttgcttatat gtaagtccgg
     1021 gtgtggcttc ggccatcttg ctcaagccaa tattcattcc cattcctaaa agggcagcca
     1081 tgataatgat tgtttcttcc ttatctggtt ttcgattatt ggaagcatga gtgaattgct
     1141 catgaaatcc tgttatatgg gccacatcca tgagtaaatc agttaatttt attcttggta
     1201 gcatctgata aaggcttgca ctaaattttt ttgcttcttc tggaacatct ttttctaagc
     1261 gtgcaagtga tagctttcct ttttcaagag aaaccccatc taacttattg gaattggcag
     1321 ctaaccactt taacctttca ttaaagctgc tggttctctc cgttatataa tcttcgaatg
     1381 ataaactaac tgataatctc gtattcccct tcgattgatt ccatgtatct tccgaaaaca
     1441 aatattcctc aaaatcccta tattgtctgc tgccaacaat ggaaacatct cctgcccgaa
     1501 catgctcccg aagttctgtt aaaacagcca tttcatagta atgacgatta attgttgtac
     1561 catcatcctc gtataaatgt cttttccatc gttttgaaat aaaatccaca ggtgagtcat
     1621 caggcacttt tcgctttcca gattcgttca ttcctcggat aatctcaaca gcttgtaaaa
     1681 gtggctcatt tgcctttgta gaatgaaatt ccaatactct taatagcgtt ggcgtatatt
     1741 ttcttagtga ataaaaccgt ttttgcagta agtctaaata atcatagtcg gcaggacgtg
     1801 caagttcctg agcctcttct actgaagaga caaaggtatt ccattcaata accgattcta
     1861 aaaccttaaa aacgtctaat ttttcctctc ttgctttaat taatgcttgt ccgatgttcg
     1921 taaagtgtat aactttctca tttagctttt taccgttttg tttctggatt tcctcttgag
     1981 ccttacgacc ttttgataac aaactaagta tttgcctatc atgaatttca aacgctttat
     2041 ccgttagctc ctgagtaagt tgtaataaat agatggttaa tatcgaataa cgtttatttt
     2101 cttgaaagtc acggaatgca tacggctcgt atcttgagcc taagcgagac agctgcaaca
     2161 ggcggttacg gtgcaaatga ctaatttgca ctgtttctaa atccattcct cgtatgtatt
     2221 cgagtcgttc tattattttt agaaaagttt cgggtgaagg atgacccggt ggctctttta
     2281 accaacccaa tatcgtttta ttggattcgg atggatgctg cgaggtaata atcccttcaa
     2341 gcttttcttt ttgctcattt gttagagatt tactaaccgt attaaatagc ttcttttcag
     2401 ccattgccct tgcttcccac accattcttt caagtgtagt gatagcaggc agtataattt
     2461 tgttttttct tagaaaatct atgcattcat gcagtagatg aatggcatca ccattttcca
     2521 aagctaattg atgaaggtac ttaaatgtca ttcgatattc actcagggta aaagttacaa
     2581 agtcgtattc acttcgaatt tctttcaaat gatcccaaag tgtattttcc ctttgaggat
     2641 aatgatcaag cgaggatgga ctaacaccaa tctgtttcga tatatattgt atgaccgaat
     2701 ctgggatgct tttgatatga gtgtatggcc aaccgggata ccgaagaaca gctaattgaa
     2761 cagcaaatcc taaacggttt tcttccctcc ttcgcttatt aactatttct aaatcccgtt
     2821 tggaaaaagt gaagtaggtc cccagtatcc attcatcttc agggatttgc ataaaagcct
     2881 gtctctgttc cggtgtaagc aattctctac ctctcgcaat tttcattcag tatcatccca
     2941 tttctgtatt ttcaatttat tagttcaatt atatatcaat agagtgtact ctattgatac
     3001 aaatgtagta gactgataaa atcatagtta agagcgtctc ataagacttg tctcaaaaat
     3061 gaggtgatat tttgcggaaa atcggttata ttcgtgtcag ttcgactaac cagaatcctt
     3121 caagacaatt tcagcagttg aacgagatcg gaatggatat tatatatgaa gagaaagttt
     3181 caggagcaac aaaggatcgc gagcaacttc aaaaagtgtt agacgattta caggaagatg
     3241 acatcattta tgttacagac ttaactcgaa tcactcgtag tacacaagat ctatttgaat
     3301 taatcgataa catacgagat aaaaaggcaa gtttaaaatc actaaaagat acatggcttg
     3361 atttatcaga agataatcca tacagccaat tcttaattac tgtaatggct ggtgttaacc
     3421 aattagagcg agatcttatt cggatgagac aacgtgaagg gattgaattg gctaagaaag
     3481 aaggaaagtt taaaggtcga ttaaagaagt atcataaaaa tcacgcagga atgaattatg
     3541 cggtaaagct atataaagaa ggaaatatga ctgtaaatca aatttgtgaa attactaatg
     3601 tatctagggc ttcattatac aggaaattat cagaagtgaa taattagcca ttctgtattc
     3661 cgctaatggg caatattttt aaagaagaaa aggaaactat aaaatattaa cagcctccta
     3721 gcgatgccga aaagcccttt gataaaaaaa gaatcatcat cttaagaaat tcttagtcat
     3781 ttattatgta aatgcttata aattcggccc tataatctga taaattatta agggcaaact
     3841 tatgtgaaag ggtgataact atgagcgata aaatacttat tgtggatgat gaacatgaaa
     3901 ttgccgattt ggttgaatta tacttaaaaa acgagaatta tacggttttc aaatactata
     3961 ccgccaaaga agcattggaa tgtatagaca agtctgagat tgaccttgcc atattggaca
     4021 tcatgcttcc cggcacaagc ggccttacta tctgtcaaaa aataagggac aagcacacct
     4081 atccgattat catgctgacc gggaaagata cagaggtaga taaaattaca gggttaacaa
     4141 tcggcgcgga tgattatata acgaagccct ttcgcccact ggagttaatt gctcgggtaa
     4201 aggcccagtt gcgccgatac aaaaaattca gtggagtaaa ggagcagaac gaaaatgtta
     4261 tcgtccactc cggccttgtc attaatgtta acacccatga gtgttatctg aacgagaagc
     4321 agttatccct tactcccacc gagttttcaa tactgcgaat cctctgtgaa aacaagggga
     4381 atgtggttag ctccgagctg ctatttcatg agatatgggg cgacgaatat ttcagcaaga
     4441 gcaacaacac catcaccgtg catatccggc atttgcgcga aaaaatgaac gacaccattg
     4501 ataatccgaa atatataaaa acggtatggg gggttggtta taaaattgaa aaataaaaaa
     4561 aacgactatt ccaaactaga acgaaaactt tacatgtata tcgttgcaat tgttgtggta
     4621 gcaattgtat tcgtgttgta tattcgttca atgatccgag ggaaacttgg ggattggatc
     4681 ttaagtattt tggaaaacaa atatgactta aatcacctgg acgcgatgaa attatatcaa
     4741 tattccatac ggaacaatat agatatcttt atttatgtgg cgattgtcat tagtattctt
     4801 attctatgtc gcgtcatgct ttcaaaattc gcaaaatact ttgacgagat aaataccggc
     4861 attgatgtac ttattcagaa cgaagataaa caaattgagc tttctgcgga aatggatgtt
     4921 atggaacaaa agctcaacac attaaaacgg actctggaaa agcgagagca ggatgcaaag
     4981 ctggccgaac aaagaaaaaa tgacgttgtt atgtacttgg cgcacgatat taaaacgccc
     5041 cttacatcca ttatcggtta tttgagcctg cttgacgagg ctccagacat gccggtagat
     5101 caaaaggcaa agtatgtgca tatcacgttg gacaaagcgt atcgactcga acagctaatc
     5161 gacgagtttt ttgagattac acggtataac ctacaaacga taacgctaac aaaaacgcac
     5221 atagacctat actatatgct ggtgcagatg accgatgaat tttatcctca gctttccgca
     5281 catggaaaac aggcggttat tcacgccccc gaggatctga ccgtgtccgg cgaccctgat
     5341 aaactcgcga gagtctttaa caacattttg aaaaacgccg ctgcatacag tgaggataac
     5401 agcatcattg acattaccgc gggcctctcc ggggatgtgg tgtcaatcga attcaagaac
     5461 actggaagca tcccaaaaga taagctagct gccatatttg aaaagttcta taggctggac
     5521 aatgctcgtt cttccgatac gggtggcgcg ggacttggat tggcgattgc aaaagaaatt
     5581 attgttcagc atggagggca gatttacgcg gaaagcaatg ataactatac gacgtttagg
     5641 gtagagcttc cagcgatgcc agacttggtt gataaaagga ggtcctaaga gatgtatata
     5701 attttggctc tttgtcaaat gatgttggta aagagaaatc tta
//
LOCUS       DBNICI010000342         2858 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.guided.222, whole genome shotgun sequence.
ACCESSION   DBNICI010000342 DBNICI010000000
VERSION     DBNICI010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.guided.222"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            104..1072
                     /locus_tag="AC1ZCC_002959"
     CDS             104..1072
                     /locus_tag="AC1ZCC_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HIQ1631147.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1065..2096
                     /gene="vanA"
                     /locus_tag="AC1ZCC_002960"
     CDS             1065..2096
                     /gene="vanA"
                     /locus_tag="AC1ZCC_002960"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HIQ1631148.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2102..2710
                     /locus_tag="AC1ZCC_002961"
     CDS             2102..2710
                     /locus_tag="AC1ZCC_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HIQ1631149.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          845 a          549 c          702 g          762 t
ORIGIN      
        1 gtaagaacga gccgttttcg ttcttcagag aaagatttga caagattacc attggcatcc
       61 ccgttttatt tggtgccttt cacagaaagg gttggtctta attatgaata acatcggcat
      121 tactgtttat ggatgtgagc aggatgaggc agatgcattc catgctcttt cgcctcgctt
      181 tggcgttatg gcaacgataa ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc
      241 tttcaatcaa tgtatcagtg tgggacataa atcagagatt tccgcctcta ttcttcttgc
      301 gctgaagaga gccggtgtga aatatatttc tacccgaagc atcggctgca atcatataga
      361 tacaactgct gctaagagaa tgggcatcac tgtcgacaat gtggcgtact cgccggatag
      421 cgttgccgat tatactatga tgctaattct tatggcagta cgcaacgtaa aatcgattgt
      481 gcgctctgtg gaaaaacatg atttcaggtt ggacagcgac cgtggcaagg tactcagcga
      541 catgacagtt ggtgtggtgg gaacgggcca gataggcaaa gcggttattg agcggctgcg
      601 aggatttgga tgtaaagtgt tggcttatag tcgcagccga agtatagagg taaactatgt
      661 accgtttgat gagttgctgc aaaatagcga tatcgttacg cttcatgtgc cgctcaatac
      721 ggatacgcac tatattatca gccacgaaca aatacagaga atgaagcaag gagcatttct
      781 tatcaatact gggcgcggtc cacttgtaga tacctatgag ttggttaaag cattagaaaa
      841 cgggaaactg ggcggtgccg cattggatgt attggaagga gaggaagagt ttttctactc
      901 tgattgcacc caaaaaccaa ttgataatca atttttactt aaacttcaaa gaatgcctaa
      961 cgtgataatc acaccgcata cggcctatta taccgagcaa gcgttgcgtg ataccgttga
     1021 aaaaaccatt aaaaactgtt tggattttga aaggagacag gagcatgaat agaataaaag
     1081 ttgcaatact gtttgggggt tgctcagagg agcatgacgt atcggtaaaa tctgcaatag
     1141 agatagccgc taacattaat aaagaaaaat acgagccgtt atacattgga attacgaaat
     1201 ctggtgtatg gaaaatgtgc gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt
     1261 cagctgtact ctcgccggat aaaaaaatgc acggattact tgttaaaaag aaccatgaat
     1321 atgaaatcaa ccatgttgat gtagcatttt cagctttgca tggcaagtca ggtgaagatg
     1381 gatccataca aggtctgttt gaattgtccg gtatcccttt tgtaggctgc gatattcaaa
     1441 gctcagcaat ttgtatggac aaatcgttga catacatcgt tgcgaaaaat gctgggatag
     1501 ctactcccgc cttttgggtt attaataaag atgataggcc ggtggcagct acgtttacct
     1561 atcctgtttt tgttaagccg gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata
     1621 gcgcggacga attggactac gcaattgaat cggcaagaca atatgacagc aaaatcttaa
     1681 ttgagcaggc tgtttcgggc tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt
     1741 tagctgttgg cgaggtggac caaatcaggc tgcagtacgg aatctttcgt attcatcagg
     1801 aagtcgagcc ggaaaaaggc tctgaaaacg cagttataac cgttcccgca gacctttcag
     1861 cagaggagcg aggacggata caggaaacgg caaaaaaaat atataaagcg ctcggctgta
     1921 gaggtctagc ccgtgtggat atgtttttac aagataacgg ccgcattgta ctgaacgaag
     1981 tcaatactct gcccggtttc acgtcataca gtcgttatcc ccgtatgatg gccgctgcag
     2041 gtattgcact tcccgaactg attgaccgct tgatcgtatt agcgttaaag gggtgataag
     2101 catggaaata ggatttactt ttttagatga aatagtacac ggtgttcgtt gggacgctaa
     2161 atatgccact tgggataatt tcaccggaaa accggttgac ggttatgaag taaatcgcat
     2221 tgtagggaca tacgagttgg ctgaatcgct tttgaaggca aaagaactgg ctgctaccca
     2281 agggtacgga ttgcttctat gggacggtta ccgtcctaat cgtgctgtaa actgttttat
     2341 gcaatgggct gcacagccgg aaaataacct gacaaaggaa agttattatc ccaatattga
     2401 ccgaactgag atgatttcaa aaggatacgt ggcttcaaaa tcaagccata gccgcggcag
     2461 tgccattgat cttacgcttt atcgattaga cacgggtgag cttgtaccaa tggggagccg
     2521 atttgatttt atggatgaac gctctcatca tgcggcaaat ggaatatcat gcaatgaagc
     2581 gcaaaatcgc agacgtttgc gctccatcat ggaaaacagt gggtttgaag catatagcct
     2641 cgaatggtgg cactatgtat taagagacga accatacccc aatagctatt ttgatttccc
     2701 cgttaaataa acttttaacc gttgcacgga caaactatat aagctaactc tttcggcagg
     2761 aaacccgacg tatgtaactg gttcttaggg aatttatata tagtagatag tattgaagag
     2821 gttctgttgc aaagttttaa ataaagaata aaatccct
//
LOCUS       DBNICI010000343         2771 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.guided.227, whole genome shotgun sequence.
ACCESSION   DBNICI010000343 DBNICI010000000
VERSION     DBNICI010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.guided.227"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            110..193
                     /locus_tag="AC1ZCC_002962"
     CDS             110..193
                     /locus_tag="AC1ZCC_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HIQ1631150.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            318..1055
                     /gene="erm(B)"
                     /locus_tag="AC1ZCC_002963"
     CDS             318..1055
                     /gene="erm(B)"
                     /locus_tag="AC1ZCC_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HIQ1631151.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1318..2181)
                     /locus_tag="AC1ZCC_002964"
     CDS             complement(1318..2181)
                     /locus_tag="AC1ZCC_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HIQ1631152.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2183..2455)
                     /locus_tag="AC1ZCC_002965"
     CDS             complement(2183..2455)
                     /locus_tag="AC1ZCC_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HIQ1631153.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2472..2681)
                     /locus_tag="AC1ZCC_002966"
     CDS             complement(2472..2681)
                     /locus_tag="AC1ZCC_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HIQ1631154.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT          851 a          487 c          443 g          990 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa attttgtata ataggaattg
       61 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      121 ccaaatgcgt aatgtagata aaacatctac cgttttgaaa cagactaaaa acagtgatta
      181 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      241 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttat agatgtaatc
      301 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      361 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      421 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      481 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      541 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      601 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      661 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      721 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      781 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      841 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      901 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      961 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1021 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt
     1081 ggaaagttac acgttactaa agggaatgta gataaattat taggtatagg gcacctctaa
     1141 taactaccaa ttaattcacc tcaaaatgaa attagaaaaa aacagcgaaa tttttaaatc
     1201 tatttcttat cgatacaaat tcctcgtagg cgctcgggac cccaacatga ggaaatgttt
     1261 ttataaaaaa agagtagtta ccgttttttg gtaactactc ttttcctttt ttaaggttta
     1321 aatacctgga agtttaggtg ttttgggtat tggtggtgta ggtagctgca agctttccat
     1381 tttttgttga attgctttaa attcaggggt ttcttggtgt tgattttgaa ccattttttg
     1441 ctctattctc tccaaagtcg gttgaatttc tttccctgat actttacgat ttaattctct
     1501 ttctaaggtc tctttcggac taatggaagg cgtttctaag cttgaataga gtttcactcc
     1561 ttctctattg taaagcctta tatcgctaaa taagcccgtt ttatgaaggg tttctaaatt
     1621 ggtcggtaag tttttgacaa caatatcatg cgcttgtttt ggtgttgccc tagctgtcat
     1681 tggatcatct gcatacatgg tttcatatcg ttcaatcgtt cctaagtatg attcaatttt
     1741 cggtactgcc atgacgtata tttttgtttc ataaccttta gattgaagca ttgtggctgt
     1801 ttgaatggga acgtctgttg ttcgtcctgt tccttcaatc actaaattat agccttgatc
     1861 gcttaaacgg ctaataagtg cttctgtcat gcgattcgaa taaggggtaa cgtgttttac
     1921 tacgtctttt tcatagagtt tcactaattc gtcaaaattt gggtgctgtt gtttaaaggt
     1981 atcattatca atgacaataa cattcccttg tgtttcttca aaaattgctg atcgcaaact
     2041 ggttttccct gaccctggtt gcccaccaag taaaaaagcg gttggcgatt caaccgctag
     2101 tctattttta gttagtcgtt caacattctt ttctaaacga tcttcaaatt gcttttcggt
     2161 gaagtcagta atattcgcca tgttaagcca ctttctcttt attcaaaatt tgttttgaat
     2221 attgattcat tgaccgccaa tactcatgaa tagcttgtaa ttcttcaaat ggttgttgaa
     2281 ataaatcaac ttcttgtgcc acttctaatt gatttaaaag attcacttct agtaaacgag
     2341 tatttttagt atctagttca tgattcaaaa cataatttaa tacccgatta taaacacttg
     2401 ccgataactc attaatgatt tcaatttcaa atgttttttc atacgtaact gccatttcat
     2461 tttcactcct tttaaagttt gtcaggtaaa tatttccgta tatattcttc taaggcttga
     2521 tccataactt cttttacgtt tccgccattc tttgctgttt cgatttttat gatatggtgc
     2581 aagtcggcac gaacacgaac cgtcttatcc cccattatat ctttttttgc gctgattggc
     2641 gtatcatttc gttttgcttt ctgtgcgcct aaatttccca cgatcgctca cttctttcta
     2701 tttcttctta ctatttattt tatcatcacc tatcacaaat cacaagtgat ttgtgattaa
     2761 tcacttgtga t
//
LOCUS       DBNICI010000344         2733 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.guided.228, whole genome shotgun sequence.
ACCESSION   DBNICI010000344 DBNICI010000000
VERSION     DBNICI010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.guided.228"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            206..841
                     /locus_tag="AC1ZCC_002967"
     CDS             206..841
                     /locus_tag="AC1ZCC_002967"
                     /inference="COORDINATES: protein motif:HMM:NF012655.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIQ1631155.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            948..2186
                     /gene="mef(H)"
                     /locus_tag="AC1ZCC_002968"
                     /gene_synonym="mefH"
     CDS             948..2186
                     /gene="mef(H)"
                     /locus_tag="AC1ZCC_002968"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HIQ1631156.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            2413..>2733
                     /locus_tag="AC1ZCC_002969"
     CDS             2413..>2733
                     /locus_tag="AC1ZCC_002969"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HIQ1631157.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDR"
BASE COUNT          908 a          378 c          487 g          960 t
ORIGIN      
        1 tctgatttgt ggtaaatcag gcaaaggcaa cgcaggtatc acagtatgaa ttgacaccgc
       61 ataaggaaag caactggaag attgtagaat aaatgaaaaa ggcttgcatt ctgcattttt
      121 ataaaattgc aagtctttat attgacaagc ataaaatact aagatatact ttaatcagac
      181 caacggtttg tattggaggt aagttatggc acgaaataaa tatccagaag aaacagtaaa
      241 actcatttta gatgtttcta caaaaatgtt ttgtgaaaaa ggatatgata acacatcatt
      301 gcaagatatt atcaatgaaa caaaactttc taaaggtgct atttatcatc atttcgattc
      361 aaaggaagat attttaaaag caatttttca aagaattgga aatgaaaatg cagtaatatt
      421 tgctaaaatt agagataacg atagtttaaa cggtttagaa aagttaagag aaatatttaa
      481 gacagcggta ttcaattcta accaaagcgt tttattgaca gtttctcctc aactattaaa
      541 caatcctcgt tttcttgcta tgcaaataga acaaatgtat gaaattgtag tacctgaatt
      601 tgttgaaccc gttttagaac aaggtatcaa ggataagtct ttggagattg aaaattcaca
      661 tgaaattgca gaagttatca tggtattaag taatgtatgg ctcaacccat tagttgaaat
      721 gacggataaa gagggtatgg aaaaacgttg caatacattc aatgcccttt taaatggaat
      781 aggcatagac acattattag acgaagaaac aatttctgca tttatacagt tttgtaaata
      841 actgtatttg ttatccctat acgatagact gctctataaa agagcagttt atttaaacca
      901 ttatataaac cgacggtctg tttgattaaa taaaaaggag agattttatg caagcagaaa
      961 aaaaactttt taatcgtgat tttatacttg ttgttatagg acagataatt tctttatttg
     1021 gaaacgctat cctacgattt gcattaccgt actatttgtt aaaagaaact ggttcatcag
     1081 ctttgtttgg tattgctaca gcctgctctt ttttacctat ggttattttg tcgctggtag
     1141 gtggtattat tgctgacagg acaaataaaa gaaacattat ggtcatacta gattttgtaa
     1201 cagcaatcat aattttagta ttttatttgt cattgggaaa aattcctatc atacctttgt
     1261 ttattgttgc tttgatgtta ctttatggta tttctggtgc atatcaacca gcggtacaag
     1321 caagtattcc tctattggta tcatcagata aacttaccgt cggtaatgct ataataaatc
     1381 aagtaaatac tttagcaaac tttatcggac cagctataag cggagtagtt tttcaatttt
     1441 atggtatcaa tcccatttta attgttagta ttatatgttt tactttttca gccattatgg
     1501 aaatttttat ccatattcca caccaaaaaa gacctcgtga agtaggtgta attgcagttg
     1561 ctaaaaacga tttaaaagat agttatacct ttttgaaaaa ggagaaaccc atttttatat
     1621 ccgtaatctt tttggtttgc attttcaatt tggttttaag tgctgtttta attgttggtc
     1681 tgcctatctt aatttctgaa gttttaaaaa tgccagatac actatatggt ttttcacaat
     1741 cagcattatc attaggtggt ttatgtggtg gtattttaac agctcttgtt gcttcaaagt
     1801 taaaactaaa aaacctttat accttgttac ttatttgttc tgtttctgtt ttacttatgg
     1861 gaatttcgct atttttagaa ttgcctgcaa tagtttccta ctgggtagtt acagtgatgt
     1921 gctttgtggc tatgggtgta tctactatgt ttgttgttca aatttacact cttgttcaga
     1981 cacaaacacc accgcaatta gtaggaaaga taatggctac gctggtagca gttgctatgt
     2041 gcggtcaacc tattggacaa atggtttacg gtattttatt cgatgtattt atacaagaaa
     2101 catgggttgt tatgtttgta gcttctattg cttcaatcat gatttcaata tattcaaaaa
     2161 gaatttttca aaagttagag atataataca aggggttaaa tagtatgtta ggctaaaaac
     2221 aatatataaa agaaaatttg aaatctgcta aaaggatagt gttataaaag aaagagtaat
     2281 attgcagtat ttaaggctcg cacaaatata tgttttgtgt gggctttttt cttaccattt
     2341 ttctgtattt ctaaagggta taaacggtat tccgtttata tatttcagca ttagcagaaa
     2401 gggggtgaga ttatgaggac atcttctttc gagcatatcg tcagattaca gttcaattct
     2461 ttaatgctga ttgtcattaa gaacaaagtg aaaagtcgaa acagacagct tgcaagacat
     2521 tctaaacgtg aagtattgtt ttgtgaaata gccgaaacga aacaagttga acgtggaacg
     2581 attgatacct gtttttatga ttgtgtatca tttaaagtct tgcactttac aatttatgta
     2641 tctgatgaaa cacttggtac agttttacat agactgtcag aaaaacagcg tagtgcgatt
     2701 ttattacgtt attttcaagg aatgaacgac aga
//
LOCUS       DBNICI010000345         2295 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.guided.240, whole genome shotgun sequence.
ACCESSION   DBNICI010000345 DBNICI010000000
VERSION     DBNICI010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.guided.240"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            complement(27..1130)
                     /locus_tag="AC1ZCC_002970"
     CDS             complement(27..1130)
                     /locus_tag="AC1ZCC_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HIQ1631158.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            1682..2230
                     /gene="aac(6')"
                     /locus_tag="AC1ZCC_002971"
     CDS             1682..2230
                     /gene="aac(6')"
                     /locus_tag="AC1ZCC_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HIQ1631159.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          727 a          404 c          450 g          714 t
ORIGIN      
        1 tgcttgatgg gggttttagc aattatttaa ctgattttct tatatccttg ctggctttct
       61 tgtgtagcat gataagtttc tacgtcaaga atggcctgtt taaatttttc tcgggtgatt
      121 tcataaggca catgtttaat atcatttgtt gccatggctt tatctaagat tgcttctaaa
      181 tcggtcgtga cattgagatt catgtcggtt aacgatactg gaagttggtt ctttttcata
      241 aacacataga ttttatcaaa caaatcgaac tgctgatcca tcatcaataa ggtcagtaca
      301 ccataactga cagatgctcc gtgaaggtat ccctccattt tttctagaac agtcgtaccg
      361 taatagaatg agtgtgccat agcagaatta tagttattgt taactaaaac agaaaccaat
      421 ccggtagtaa caatgatatc cgaaacgact tgttcaattg catatgaggg acgatcgtcg
      481 gcactatctt tcaatgcttg cgtaccataa gtcaagagac gatcaatgca catttttccg
      541 atttggattc ctaatctatc cgtgtagcct agatcatcgc cacgagcaga aaaagtagat
      601 tcgacttctt tggataaagc atcgccaata cctgcccata agtatttatc aggtgcttcg
      661 gcaataattg ctgtatcaat gaaggtgtgt acaggtggtt ttgaaggaaa gaagagtccg
      721 tccattacgc cttcttcgtt atagatgaca cataccgcag tgacgggcga acagttcgaa
      781 ccaatagtgg gaaaggtgaa gactggttta gctagttttt caccaatgac ttttacagta
      841 tcgcaagcac ggccaccacc gactgcaaac aggacgtctg ctttttggac actttcatgc
      901 tggatcaact gttcaatatt tccataagtt gaattacccc cgtaccagag actggcgaca
      961 acctctattt cctgtgtcaa agctactttt attttgtttt ctgcctttga aagtgcagtc
     1021 ttccctccaa tcagcactac ttttgtccca tattctttga cgattccatt tgctttttca
     1081 tacgcatctg ttccaatggt ataatttggt aagaaaacac tttgactcat attcatttcc
     1141 ttctttcttc gttgaaacgg gttaaaattt taaagagtga tcgatctgac tgcggctgta
     1201 gatggagtag aggtgaacct aagacaggat aagtgatgaa taaggtggca tgaacacctt
     1261 gaaatcatca ataagtatcc taaaaacgaa caagtacaaa aacattgaaa acgacaataa
     1321 tgaatttttg ttcctcttag tatttctacc gattcccttt gcttttttgt aattatctaa
     1381 aaattctaaa aattaagatt attatatgaa aacaggtttt atttgtcaat tcataattgg
     1441 ttttttttaa agcaaaagat aatctatatc atgatgaaca aacagagttt tttcctccaa
     1501 ttgatggctt tcctgatctt tcctattttg aataaagtgt ttctgtataa ttttttattt
     1561 tataagtaag ataaataatg aaagaataga gatactgatt ggtagctaaa gatttttgag
     1621 cgagaacatt tcgttttttc aaaaggactg ttagaataag caagaacaag ggggaagacg
     1681 tatgataatc agtgaatttg accgtaataa tccagtattg aaagatcagc tttctgattt
     1741 actgagactg acttggccgg aagaatatgg agacagctcg gcagaagaag tagaagaaat
     1801 gatgaatcca gaacgaatcg cggtagcagc ggtagaccaa gatgagttag taggatttat
     1861 tggtgcaatc cctcaatacg gtatcacagg ttgggaattg catccattag ttgtagaaag
     1921 ctcccgacga aagaaccaaa taggtactcg attagtcaat tacttagaaa aagaagtagc
     1981 ttccagagga ggaatcacga tttatttagg tacggatgat ttagaccatg gaacaacgtt
     2041 aagtcaaacg gacctgtatg agcatacatt tgataaagtg gcttccatcc agaaccttcg
     2101 tgaacatccg tatgaattct atgaaaaatt aggttataaa atcgtaggtg tcttaccaaa
     2161 tgcaaatggc tgggacaaac cggatatttg gatggcaaaa acgattattc ctcgaccaaa
     2221 ttctcaataa taaaaagagg ttgtgacaga agtaatcagc ttcaagaaat aagaaggaat
     2281 ttacgaaaat tactt
//
LOCUS       DBNICI010000346         2046 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.guided.245, whole genome shotgun sequence.
ACCESSION   DBNICI010000346 DBNICI010000000
VERSION     DBNICI010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.guided.245"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            76..1995
                     /gene="tet(M)"
                     /locus_tag="AC1ZCC_002972"
     CDS             76..1995
                     /gene="tet(M)"
                     /locus_tag="AC1ZCC_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HIQ1631160.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT          699 a          316 c          423 g          608 t
ORIGIN      
        1 aaaaatccca gtgataagag tatttatcac tgggattttt atgccctttt gggcttttga
       61 atggaggaaa atcacatgaa aattattaat attggagttt tagctcatgt tgatgcggga
      121 aaaactacct taacagaaag cttattatat aacagtggag cgattacaga attaggaagc
      181 gtggacaaag gtacaacgag gacggataat acgcttttag aacgtcagag aggaattaca
      241 attcagacag gaataacctc ttttcagtgg gaaaatacga aggtgaacat catagacacg
      301 ccaggacata tggatttctt agcagaagta tatcgttcat tatcagtttt agatggggca
      361 attctactga tttctgcaaa agatggcgta caagcacaaa ctcgtatatt atttcatgca
      421 cttaggaaaa tggggattcc cacaatcttt tttatcaata agattgacca aaatggaatt
      481 gatttatcaa cggtttatca ggatattaaa gagaaacttt ctgccgaaat tgtaatcaaa
      541 cagaaggtag aactgtatcc taatatgtgt gtgacgaact ttaccgaatc tgaacaatgg
      601 gatacggtaa tagagggaaa cgatgacctt ttagagaaat atacgtctgg gaaattattg
      661 gaagcattag aactcgaaca agaggaaagc ataagatttc ataattgttc cctgttccct
      721 gtttatcacg gaagtgcaaa aaacaatata gggattgata accttataga agtgattacg
      781 aataaatttt attcatcaac acatcgaggt cagtctgaac tttgcggaaa agttttcaaa
      841 attgagtatt cggaaaaaag acagcgtctt gcatatatac gtctttatag tggcgtactg
      901 catttgcgag attcggttag aatatcggaa aaggaaaaaa taaaaattac agaaatgtat
      961 acttcaataa atggtgaatt atgtaaaatc gataaggctt attccgggga aattgttatt
     1021 ttgcaaaatg agtttttgaa gttaaatagt gttcttggag atacaaaact attgccacag
     1081 agaaaaaaga ttgaaaatcc gcaccctcta ctacaaacaa ctgttgaacc gagtaaacct
     1141 gaacagagag aaatgttgct tgatgccctt ttggaaatct cagatagtga tccgcttcta
     1201 cgatattacg tggattctac gacacatgaa attatacttt ctttcttagg gaaagtacaa
     1261 atggaagtga ttagtgcact gttgcaagaa aagtatcatg tggagataga aataaaagag
     1321 cctacagtca tttatatgga aagaccgtta aaaaaagcag agtataccat tcacatcgaa
     1381 gttccaccga atcctttctg ggcttccatt ggtctatctg tagcacagct tccattaggg
     1441 agcggagtac agtatgagag ctcggtttct cttggatact taaatcaatc gtttcaaaat
     1501 gcagttatgg aggggatacg ctatggctgt gaacaaggat tgtatggttg gaatgtgacg
     1561 gactgtaaaa tctgttttaa gtatggctta tactatagcc ctgttagtac cccagcagat
     1621 tttcggatgc ttgctcctat tgtattggaa caagtcttaa aaaaagctgg aacagaattg
     1681 ttagagccat atcttagttt taaaatttat gcgccacagg aatatctttc acgagcatac
     1741 aacgatgctc ctaaatattg tgcgaacatc gtagacactc aattgaaaaa taatgaggtc
     1801 attcttagtg gagaaatccc tgctcggtgt attcaagaat atcgtagtga tttaactttc
     1861 tttacaaatg gacgtagtgt ttgtttaaca gagttaaaag ggtaccatgt tactaccggt
     1921 gaacctgttt gccagccccg tcgtccaaat agtcggatag ataaagtacg atatatgttc
     1981 aataaaataa cttagtgtat tttatgttgt tatataaata tggtttcttg ttaaataaga
     2041 tgaaat
//
LOCUS       DBNICI010000347         2040 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.guided.247, whole genome shotgun sequence.
ACCESSION   DBNICI010000347 DBNICI010000000
VERSION     DBNICI010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.guided.247"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            202..1113
                     /locus_tag="AC1ZCC_002973"
     CDS             202..1113
                     /locus_tag="AC1ZCC_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HIQ1631161.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1266..1751
                     /gene="vanZ-A"
                     /locus_tag="AC1ZCC_002974"
     CDS             1266..1751
                     /gene="vanZ-A"
                     /locus_tag="AC1ZCC_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HIQ1631162.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
BASE COUNT          722 a          285 c          388 g          645 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa ttcatctaca ttggtgataa
       61 tagtaaatcc agtagggcga aataattgac tgtaatttac ggggcaaaac ggcacaatct
      121 caaacgagat tgtgccgttt aaggggaaga ttctagaaat atttcatact tccaactata
      181 tagttaagga ggagactgaa aatgaagaag ttgttttttt tattgttatt gttattctta
      241 atatacttag gttatgacta cgttaatgaa gcactgtttt ctcaggaaaa agtcgaattt
      301 caaaattatg atcaaaatcc caaagaacat ttagaaaata gtgggacttc tgaaaatacc
      361 caagagaaaa caattacaga agaacaggtt tatcaaggaa atctgctatt aatcaatagt
      421 aaatatcctg ttcgccaaga aagtgtgaag tcagatatcg tgaatttatc taaacatgac
      481 gaattaataa atggatacgg gttgcttgat agtaatattt atatgtcaaa agaaatagca
      541 caaaaatttt cagagatggt caatgatgct gtaaagggtg gcgttagtca ttttattatt
      601 aatagtggct atcgagactt tgatgagcaa agtgtgcttt accaagaaat gggggctgag
      661 tatgccttac cagcaggtta tagtgagcat aattcaggtt tatcactaga tgtaggatca
      721 agcttgacga aaatggaacg agcccctgaa ggaaagtgga tagaagaaaa tgcttggaaa
      781 tacgggttca ttttacgtta tccagaggac aaaacagagt taacaggaat tcaatatgaa
      841 ccatggcata ttcgctatgt tggtttacca catagtgcga ttatgaaaga aaagaatttc
      901 gttctcgagg aatatatgga ttacctaaaa gaagaaaaaa ccatttctgt tagtgtaaat
      961 ggggaaaaat atgagatctt ttattatcct gttactaaaa ataccaccat tcatgtgccg
     1021 actaatcttc gttatgagat atcaggaaac aatatagacg gtgtaattgt gacagtgttt
     1081 cccggatcaa cacatactaa ttcaaggagg taaggatggc ggaatgaaac caacgaaatt
     1141 aatgaacagc attattgtac tagcactttt ggggtaacgt tagcttttta atttaaaacc
     1201 cacgttaact aggacattgc tatactaatg atacaactta aacaaaagaa ttagaggaaa
     1261 ttatattggg aaaaatatta tctagaggat tgctagcttt atatttagtg acactaatct
     1321 ggttagtgtt attcaaatta caatacaata ttttatcagt atttaattat catcaaagaa
     1381 gtcttaactt gactccattt actgctactg ggaatttcag agagatgata gataatgtta
     1441 taatctttat tccatttggc ttgcttttga atgtcaattt taaagaaatc ggatttttac
     1501 ctaagtttgc ttttgtactg gttttaagtc ttacttttga aataattcaa tttatcttcg
     1561 ctattggagc gacagacata acagatgtaa ttacaaatac tgttggaggc tttcttggac
     1621 tgaaattata tggtttaagc aataagcata tgaatcaaaa aaaattagac agagttatta
     1681 tttttgtagg tatacttttg ctcgtattat tgctcgttta ccgtacccat ttaagaataa
     1741 attacgtgta agatgtctaa atcaagcaat ctgatctttc atacacataa agatattgaa
     1801 tgaattggat tagatggaaa acgggatgtg gggaaactcg cccgtaggtg tgaagtgagg
     1861 ggaaaaccgg tgataaagta aaaagcttac ctaacactat agtaacaaag aaagcccaat
     1921 tatcaatttt agtgctgagg aattggtctc tttaataaat ttccttaacg ttgtaaatcc
     1981 gcattttcct gacggtaccc ccaaactaag atttcataga tagattttct gataacttta
//
LOCUS       DBNICI010000348         1344 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.guided.273, whole genome shotgun sequence.
ACCESSION   DBNICI010000348 DBNICI010000000
VERSION     DBNICI010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.guided.273"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            87..584
                     /gene="dfrG"
                     /locus_tag="AC1ZCC_002975"
     CDS             87..584
                     /gene="dfrG"
                     /locus_tag="AC1ZCC_002975"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HIQ1631163.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(656..>1344)
                     /locus_tag="AC1ZCC_002976"
     CDS             complement(656..>1344)
                     /locus_tag="AC1ZCC_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HIQ1631164.1"
                     /translation="ISDDDFSIRNAFYRVFTKELRLSDAHHFICQVNKTKSRKQCLKE
                     FGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEEAISKDGERYRKYADNP
                     IKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTNSFIQQIRRYISSLERP
                     LTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSADKKVLTPAQRLGITDKQ
                     FDWKDIIYFK"
BASE COUNT          444 a          192 c          261 g          447 t
ORIGIN      
        1 caactaacgg gcaggataat gagaaaaggt tttttgatag aaataagaaa ttagatggta
       61 aattagttaa aaaggctgtg ttataaatga aagtttcttt gattgctgcg atggataaga
      121 atagagtgat tggcaaagag aatgacattc cttggaggat tcccaaggac tgggaatatg
      181 ttaaaaatac tacaaaggga catccgataa tattaggtag gaagaacctt gaatcaatcg
      241 gaagagcctt acctgacaga agaaatatta ttctgacgag agataagggg tttaccttta
      301 atggttgtga aattgttcat tcaatagaag atgtttttga gttatgtaaa aacgaagaag
      361 aaatttttat tttcggagga gaacagattt ataatttgtt tttcccttat gttgagaaaa
      421 tgtacatcac aaaaatacat catgaattcg aaggagatac tttttttcca gaagtgaatt
      481 atgaggaatg gaatgaggta tttgcccaaa aagggataaa gaatgataaa aatccgtata
      541 actactattt tcatgtatat gaaagaaaaa acttattgag ttaacgggtg cgttaattga
      601 agaagaacag taaagataaa gcacaagagc ttgggattac tccaagctct ttttgtcatt
      661 tgaaataaat tatatctttc caatcaaatt gtttatctgt aatccctaat ctttgagcag
      721 gtgtcaaaac ctttttatcc gctgatttaa aagggcgaca aaagttgtag taagttctta
      781 gaattgtaat cgcaaattga gcatacttcg gattgaagtt agcgtaaata tagctcttct
      841 tatcaccacg agctgttgtt aatggtcttt caagagatga tatataacgt cttatctgtt
      901 gaataaagct attggtcgaa tggtcattaa catttaatag catctttgcg atttcgtttg
      961 gttccagagc agataaatcc gttctacaat caactgaata aaatccttta tccttagtgg
     1021 ctaatggatg tttgataggg ttatcggcat atttgcgata tctttctcca tctttactaa
     1081 tagcctcttc gtggaattga tgacttgtta gtaattcagt tagataatga gatgccaaag
     1141 tccgtagaaa ctttgttttg aatcctctga tatctcccca atcgagcagt tctgcttttg
     1201 cttgtccgaa ttcttttaag cattgttttc tgcttttcgt cttgttaact tggcaaataa
     1261 aatgatgtgc atcgctcaac cttaattctt tcgtgaatac tctataaaag gcgtttctaa
     1321 tagagaagtc atcatcacta atca
//
LOCUS       DBNICI010000349         1068 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.guided.295, whole genome shotgun sequence.
ACCESSION   DBNICI010000349 DBNICI010000000
VERSION     DBNICI010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.guided.295"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            <1..850
                     /locus_tag="AC1ZCC_002977"
                     /note="aph(2'')-Ia"
     CDS             <1..850
                     /locus_tag="AC1ZCC_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HIQ1631165.1"
                     /translation="FTQNILHSQIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEK
                     AIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLK
                     RDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMER
                     LNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEE
                     EIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIY
                     KRTYKD"
BASE COUNT          452 a           87 c          166 g          363 t
ORIGIN      
        1 atttacacaa aatattttac actctcaaat aatcggtagt ggttatgata gtgtggcata
       61 tttagttaat aatgaataca tttttaaaac aaaatttagt actaataaga aaaaaggtta
      121 tgcaaaagaa aaagcaatat ataatttttt aaatacaaat ttagaaacta atgtaaaaat
      181 tcctaatatt gaatattcgt atattagtga tgaattatct atactaggtt ataaagaaat
      241 taaaggaact tttttaacac cagaaattta ttctactatg tcagaagaag aacaaaattt
      301 gttaaaacga gatattgcca gttttttaag acaaatgcac ggtttagatt atacagatat
      361 tagtgaatgt actattgata ataaacaaaa tgtattagaa gagtatatat tgttgcgtga
      421 aactatttat aatgatttaa ctgatataga aaaagattat atagaaagtt ttatggaaag
      481 actaaatgca acaacagttt ttgagggtaa aaagtgttta tgccataatg attttagttg
      541 taatcatcta ttgttagatg gcaataatag attaactgga ataattgatt ttggagattc
      601 tggaattata gatgaatatt gtgattttat atacttactt gaagatagtg aagaagaaat
      661 aggaacaaat tttggagaag atatattaag aatgtatgga aatatagata ttgagaaagc
      721 aaaagaatat caagatatag ttgaagaata ttatcctatt gaaactattg tttatggaat
      781 taaaaatatt aaacaggaat ttatcgaaaa tggtagaaaa gaaatttata aaaggactta
      841 taaagattga ttatataata tatgaaaagc tattataaaa gacattagta ttaaatagtt
      901 taaaaaaatg aaaaataata aaggaagtga gtcaattcca gactcctgtg taaaatgcta
      961 tacaatgttt ttaccatttc tacttatcaa aattgatgta ttttcttgaa gaataaatcc
     1021 attcatcatg taggtccata agaacggttc tgttgcaaag ttttaaat
//
LOCUS       DBNICI010000350          721 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22960
            SAMN57369376-rid29498433.guided.322, whole genome shotgun sequence.
ACCESSION   DBNICI010000350 DBNICI010000000
VERSION     DBNICI010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369376
            Sequence Read Archive: SRR38189139
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183849.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/22/2026 00:25:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,873
            CDSs (with protein)               :: 2,873
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369376-rid29498433.guided.322"
                     /strain="EF22960"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-09-24"
     gene            182..531
                     /locus_tag="AC1ZCC_002978"
                     /pseudo
     CDS             182..531
                     /locus_tag="AC1ZCC_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
BASE COUNT          239 a          125 c          104 g          253 t
ORIGIN      
        1 atattaaaga gtaaaaagat accccaaatc cctattagta acaagatcag ttctttgtta
       61 ttcgttattt ttctattatg tttgttcatt tttatactcc ttcttatatt tcctaataga
      121 gtataacata gctttaagca ttacttaaag ataacttaaa agttatcttt taaatactta
      181 attgttaaat accttatttg atgcaaaaga aatctacgat agtcaacaaa aaaattttca
      241 ttatatccag aaaaattttt cttagttaag gacctatgga ttgcagtaat gcagaattcg
      301 tcgaataaac tgccaaagac atacaatcat attgctttta aaattgacga acaagatata
      361 gattcgtttg tctcaaaaat ccaaatgtta ggtttaactg tcgaaccagg acgttctcgt
      421 gtgaaaggcg aggcttcttc aatttacttt tacgactatg ataatcacct cttcgagtta
      481 cataccggta ctctccagga acgtttaaaa tcctacaatt ccactacctg agactagtaa
      541 tgactagtct ctttttatgg cctctgatcc ccccattgag cgaatactaa tagggcttcg
      601 aattgatctg ttcaatcttt aagcaatatc cctcaaatga gtttggaaag tttagtgatt
      661 catcacaaat acttaaatac atgtagttat atatagattg aggttcttgt gagcgtatgc
      721 t
//
